LOCUS       DACCKU010000001        87232 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.001, whole genome shotgun sequence.
ACCESSION   DACCKU010000001 DACCKU010000000
VERSION     DACCKU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 87232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.001"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(29..376)
                     /gene="rbfA"
                     /locus_tag="IVN13_000001"
     CDS             complement(29..376)
                     /gene="rbfA"
                     /locus_tag="IVN13_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP7010530.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(403..2709)
                     /gene="infB"
                     /locus_tag="IVN13_000002"
     CDS             complement(403..2709)
                     /gene="infB"
                     /locus_tag="IVN13_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP7010531.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2722..3033)
                     /locus_tag="IVN13_000003"
     CDS             complement(2722..3033)
                     /locus_tag="IVN13_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP7010532.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3030..3323)
                     /locus_tag="IVN13_000004"
     CDS             complement(3030..3323)
                     /locus_tag="IVN13_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP7010533.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3345..4520)
                     /gene="nusA"
                     /locus_tag="IVN13_000005"
     CDS             complement(3345..4520)
                     /gene="nusA"
                     /locus_tag="IVN13_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP7010534.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4543..5016)
                     /gene="rimP"
                     /locus_tag="IVN13_000006"
     CDS             complement(4543..5016)
                     /gene="rimP"
                     /locus_tag="IVN13_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP7010535.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5161..9513)
                     /locus_tag="IVN13_000007"
     CDS             complement(5161..9513)
                     /locus_tag="IVN13_000007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP7010536.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            complement(9725..11434)
                     /locus_tag="IVN13_000008"
     CDS             complement(9725..11434)
                     /locus_tag="IVN13_000008"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP7010537.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            complement(11502..12770)
                     /gene="rseP"
                     /locus_tag="IVN13_000009"
     CDS             complement(11502..12770)
                     /gene="rseP"
                     /locus_tag="IVN13_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP7010538.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12931..13731)
                     /locus_tag="IVN13_000010"
     CDS             complement(12931..13731)
                     /locus_tag="IVN13_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP7010539.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13728..14540)
                     /locus_tag="IVN13_000011"
     CDS             complement(13728..14540)
                     /locus_tag="IVN13_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP7010540.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14735..15292)
                     /gene="frr"
                     /locus_tag="IVN13_000012"
     CDS             complement(14735..15292)
                     /gene="frr"
                     /locus_tag="IVN13_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP7010541.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15295..16017)
                     /locus_tag="IVN13_000013"
     CDS             complement(15295..16017)
                     /locus_tag="IVN13_000013"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP7010542.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16153..17034)
                     /locus_tag="IVN13_000014"
     CDS             complement(16153..17034)
                     /locus_tag="IVN13_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP7010543.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17133..17915)
                     /gene="rpsB"
                     /locus_tag="IVN13_000015"
     CDS             complement(17133..17915)
                     /gene="rpsB"
                     /locus_tag="IVN13_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP7010544.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18274..18753
                     /locus_tag="IVN13_000016"
     CDS             18274..18753
                     /locus_tag="IVN13_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP7010545.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18970..20061
                     /gene="phnW"
                     /locus_tag="IVN13_000017"
     CDS             18970..20061
                     /gene="phnW"
                     /locus_tag="IVN13_000017"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP7010546.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20054..20707
                     /locus_tag="IVN13_000018"
     CDS             20054..20707
                     /locus_tag="IVN13_000018"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP7010547.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQN"
     gene            complement(21184..22875)
                     /locus_tag="IVN13_000019"
     CDS             complement(21184..22875)
                     /locus_tag="IVN13_000019"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP7010548.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23297..24244)
                     /gene="arcC"
                     /locus_tag="IVN13_000020"
     CDS             complement(23297..24244)
                     /gene="arcC"
                     /locus_tag="IVN13_000020"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP7010549.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEAHVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24359..25378)
                     /gene="argF"
                     /locus_tag="IVN13_000021"
     CDS             complement(24359..25378)
                     /gene="argF"
                     /locus_tag="IVN13_000021"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP7010550.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25469..26698)
                     /gene="arcA"
                     /locus_tag="IVN13_000022"
     CDS             complement(25469..26698)
                     /gene="arcA"
                     /locus_tag="IVN13_000022"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP7010551.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27159..27860
                     /locus_tag="IVN13_000023"
     CDS             27159..27860
                     /locus_tag="IVN13_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7010552.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(28432..29448)
                     /gene="tsaD"
                     /locus_tag="IVN13_000024"
     CDS             complement(28432..29448)
                     /gene="tsaD"
                     /locus_tag="IVN13_000024"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP7010553.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(29445..29909)
                     /gene="rimI"
                     /locus_tag="IVN13_000025"
     CDS             complement(29445..29909)
                     /gene="rimI"
                     /locus_tag="IVN13_000025"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7010554.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(29916..30458)
                     /gene="rimI"
                     /locus_tag="IVN13_000026"
     CDS             complement(29916..30458)
                     /gene="rimI"
                     /locus_tag="IVN13_000026"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP7010555.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(30442..31266)
                     /gene="tsaB"
                     /locus_tag="IVN13_000027"
     CDS             complement(30442..31266)
                     /gene="tsaB"
                     /locus_tag="IVN13_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP7010556.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(31355..32335)
                     /locus_tag="IVN13_000028"
     CDS             complement(31355..32335)
                     /locus_tag="IVN13_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7010557.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(32359..33843)
                     /gene="galT"
                     /locus_tag="IVN13_000029"
     CDS             complement(32359..33843)
                     /gene="galT"
                     /locus_tag="IVN13_000029"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP7010558.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(33855..34844)
                     /gene="galE"
                     /locus_tag="IVN13_000030"
     CDS             complement(33855..34844)
                     /gene="galE"
                     /locus_tag="IVN13_000030"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP7010559.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            35093..35260
                     /locus_tag="IVN13_000031"
     CDS             35093..35260
                     /locus_tag="IVN13_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP7010560.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(35322..37133)
                     /locus_tag="IVN13_000032"
     CDS             complement(35322..37133)
                     /locus_tag="IVN13_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP7010561.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(37130..37495)
                     /locus_tag="IVN13_000033"
     CDS             complement(37130..37495)
                     /locus_tag="IVN13_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP7010562.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(37658..38053)
                     /locus_tag="IVN13_000034"
     CDS             complement(37658..38053)
                     /locus_tag="IVN13_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7010563.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(38071..39033)
                     /locus_tag="IVN13_000035"
     CDS             complement(38071..39033)
                     /locus_tag="IVN13_000035"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP7010564.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(39033..39245)
                     /locus_tag="IVN13_000036"
     CDS             complement(39033..39245)
                     /locus_tag="IVN13_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP7010565.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(39266..39964)
                     /locus_tag="IVN13_000037"
     CDS             complement(39266..39964)
                     /locus_tag="IVN13_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7010566.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(39984..40526)
                     /locus_tag="IVN13_000038"
     CDS             complement(39984..40526)
                     /locus_tag="IVN13_000038"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP7010567.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTFSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(40658..41662)
                     /locus_tag="IVN13_000039"
     CDS             complement(40658..41662)
                     /locus_tag="IVN13_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7010568.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFVLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(41659..42648)
                     /locus_tag="IVN13_000040"
     CDS             complement(41659..42648)
                     /locus_tag="IVN13_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP7010569.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(42645..43451)
                     /locus_tag="IVN13_000041"
     CDS             complement(42645..43451)
                     /locus_tag="IVN13_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7010570.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            43617..44573
                     /locus_tag="IVN13_000042"
     CDS             43617..44573
                     /locus_tag="IVN13_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7010571.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACFPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(44650..45168)
                     /locus_tag="IVN13_000043"
     CDS             complement(44650..45168)
                     /locus_tag="IVN13_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7010572.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(45256..45405)
                     /gene="rpmG"
                     /locus_tag="IVN13_000044"
     CDS             complement(45256..45405)
                     /gene="rpmG"
                     /locus_tag="IVN13_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7010573.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            45635..46081
                     /locus_tag="IVN13_000045"
     CDS             45635..46081
                     /locus_tag="IVN13_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP7010574.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDSRAIPIA
                     IDQAKEWYENASESERRTFEKEKNPSKSDKHDTNPRAGKLLDSDVIVEYAEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLVIYKKDGTKEKEIDY"
     gene            complement(46275..48170)
                     /gene="asnB"
                     /locus_tag="IVN13_000046"
     CDS             complement(46275..48170)
                     /gene="asnB"
                     /locus_tag="IVN13_000046"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP7010575.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(48495..49469)
                     /locus_tag="IVN13_000047"
     CDS             complement(48495..49469)
                     /locus_tag="IVN13_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7010576.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVANYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNVDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLDVYSRGMGGLGLPGVL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(49808..49895)
                     /locus_tag="IVN13_000048"
     tRNA            complement(49808..49895)
                     /locus_tag="IVN13_000048"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(49861..49863),aa:Ser,seq:cga)
     gene            complement(50040..50606)
                     /locus_tag="IVN13_000049"
     CDS             complement(50040..50606)
                     /locus_tag="IVN13_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7010577.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFCEIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            50862..52193
                     /locus_tag="IVN13_000050"
     CDS             50862..52193
                     /locus_tag="IVN13_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP7010578.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGAFFLFIGLLSGTPT
                     DNWSGLLTILTSPSNLLTDYFDLGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVSNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            52159..52512
                     /locus_tag="IVN13_000051"
     CDS             52159..52512
                     /locus_tag="IVN13_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010579.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASPRSYY"
     gene            complement(52604..53023)
                     /locus_tag="IVN13_000052"
     CDS             complement(52604..53023)
                     /locus_tag="IVN13_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP7010580.1"
                     /translation="MMTKYEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLRAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITSGNSEFLEPVTDEVYEGLENQ"
     gene            complement(53024..53368)
                     /locus_tag="IVN13_000053"
     CDS             complement(53024..53368)
                     /locus_tag="IVN13_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP7010581.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISLLISGGNFEQLPAHLKIGKQNGITGEEIAEIITHLAFYVGWPKAWSAFNLAKEI
                     YLEGNSVNKKEA"
     gene            53718..54314
                     /locus_tag="IVN13_000054"
     CDS             53718..54314
                     /locus_tag="IVN13_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7010582.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            54438..56237
                     /locus_tag="IVN13_000055"
     CDS             54438..56237
                     /locus_tag="IVN13_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7010583.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMMILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSFFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSIVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(56515..56727)
                     /locus_tag="IVN13_000056"
     CDS             complement(56515..56727)
                     /locus_tag="IVN13_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010584.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            57605..57991
                     /locus_tag="IVN13_000057"
     CDS             57605..57991
                     /locus_tag="IVN13_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7010585.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            58035..58247
                     /locus_tag="IVN13_000058"
     CDS             58035..58247
                     /locus_tag="IVN13_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7010586.1"
                     /translation="MLILENKKIEWVFEVTFLFFQSLLEDIKDFLGHKDISTTQVYAH
                     ISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(58352..59698)
                     /locus_tag="IVN13_000059"
     CDS             complement(58352..59698)
                     /locus_tag="IVN13_000059"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP7010587.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(59810..61159)
                     /gene="gdhA"
                     /locus_tag="IVN13_000060"
     CDS             complement(59810..61159)
                     /gene="gdhA"
                     /locus_tag="IVN13_000060"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP7010588.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKGVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHACRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            61276..62529
                     /locus_tag="IVN13_000061"
     CDS             61276..62529
                     /locus_tag="IVN13_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7010589.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAV
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHLLTEKSKTTKKNLVNAWHGLLPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIHPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            complement(62600..63085)
                     /gene="ybaK"
                     /locus_tag="IVN13_000062"
     CDS             complement(62600..63085)
                     /gene="ybaK"
                     /locus_tag="IVN13_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP7010590.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(63108..63866)
                     /gene="aroD"
                     /locus_tag="IVN13_000063"
     CDS             complement(63108..63866)
                     /gene="aroD"
                     /locus_tag="IVN13_000063"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP7010591.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            complement(63882..65060)
                     /locus_tag="IVN13_000064"
     CDS             complement(63882..65060)
                     /locus_tag="IVN13_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP7010592.1"
                     /translation="MKVQVKKRASSRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEELFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIIDYYAGYAVFSWYNETLYAHRGLLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            65290..67410
                     /locus_tag="IVN13_000065"
     CDS             65290..67410
                     /locus_tag="IVN13_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7010593.1"
                     /translation="MKYIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     VQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQIQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSQHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            67633..68358
                     /locus_tag="IVN13_000066"
     CDS             67633..68358
                     /locus_tag="IVN13_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010594.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            68348..68857
                     /locus_tag="IVN13_000067"
     CDS             68348..68857
                     /locus_tag="IVN13_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010595.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKEDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(68927..70375)
                     /locus_tag="IVN13_000068"
     CDS             complement(68927..70375)
                     /locus_tag="IVN13_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7010596.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFTVRLPDVEIKEE"
     gene            complement(70375..71091)
                     /locus_tag="IVN13_000069"
     CDS             complement(70375..71091)
                     /locus_tag="IVN13_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7010597.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(71072..71422)
                     /locus_tag="IVN13_000070"
     CDS             complement(71072..71422)
                     /locus_tag="IVN13_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP7010598.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            71566..72339
                     /locus_tag="IVN13_000071"
     CDS             71566..72339
                     /locus_tag="IVN13_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7010599.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            72517..72883
                     /gene="ssrA"
                     /locus_tag="IVN13_000072"
     tmRNA           72517..72883
                     /gene="ssrA"
                     /locus_tag="IVN13_000072"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            73103..73405
                     /locus_tag="IVN13_000073"
     CDS             73103..73405
                     /locus_tag="IVN13_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010600.1"
                     /translation="MLMEGKTQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDNLLINL
                     YYSNKNYFKIPAAKTRMKKDVYFLFDVVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            73630..73941
                     /locus_tag="IVN13_000074"
     CDS             73630..73941
                     /locus_tag="IVN13_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein"
                     /protein_id="HAP7010601.1"
                     /translation="MVKENLELEDIHQKSKVIANEVMVTASKASVPLTSNEKADIEQV
                     FSEKTIELSERADRILKDQPSLSENELAIKLIKEDLKNASMFSPMKRILKKAIKHLEE
                     N"
     gene            complement(74130..74936)
                     /locus_tag="IVN13_000075"
     CDS             complement(74130..74936)
                     /locus_tag="IVN13_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7010602.1"
                     /translation="MARHHSATISGDVWAFQNHWRGSLGWGTGGYHEIILRDGTVQLC
                     YRDNDVTNGVGNHNTVTYHICLVGNGSFTEAQERAFDVRARDAMRRFGLTSANVLGHN
                     EFSGHGSNQCPGVNMSNVRRRLADTAQTHDEIIAASAPKTVGNYVGKLEKFNELKLGV
                     FRIGAWLVPQNGAPSLGYGFVFWMDADNPNVEVGRCRSAGIVRDDVNAAYGLPSGLRF
                     GLDGTLDIRRFAGRKVFPMLRRTNDPNGDTINGQTVDIRFPEYVLTIPNR"
     gene            complement(75073..75330)
                     /locus_tag="IVN13_000076"
     CDS             complement(75073..75330)
                     /locus_tag="IVN13_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010603.1"
                     /translation="MEQILTAASIITPLVIAVTGLIKTQVNNYKILPVINVIAGIFLG
                     VLYALSFLQDAVVLYAWAGAVSGLAAGGLFDLGVSVIKKEK"
     gene            complement(75333..75566)
                     /locus_tag="IVN13_000077"
     CDS             complement(75333..75566)
                     /locus_tag="IVN13_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010604.1"
                     /translation="MSAVSITIAEWIAILTLASTVIFGVAKFYAVFTDLRKTLDRLNG
                     TIQGLQESQSEYGNRLVAIEEQIKTLFKQIGGE"
     gene            complement(75612..76421)
                     /locus_tag="IVN13_000078"
     CDS             complement(75612..76421)
                     /locus_tag="IVN13_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HAP7010605.1"
                     /translation="MKKFVKLASLSISIILFSACNLTTGFNWLNNKETILSAHRGAHT
                     AAPENTIEALEAAIDMNYGAIEIDPRMSSDGNMFLMHDDTVDRTTDGAGPLEKMTSEQ
                     IENLKIDTTDYPEFSNKTVKVPTFDEAVKVISKSDLILNVDGSKGDWLNKEFVDNIVH
                     TVKSHDMFEKTFFVLSDKKEREFFNKLYPEACVSWLFDSNSTIEKEIEKAKNYKHAML
                     SVSNDFATKEVIEKLNKSKIYYQVYSVNTQERFEELKSWYAKMIETDTLKP"
     gene            complement(77023..77337)
                     /locus_tag="IVN13_000079"
     CDS             complement(77023..77337)
                     /locus_tag="IVN13_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010606.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VFSQTIFYGGIISISLLSSTLYSLNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NK"
     gene            77623..77862
                     /locus_tag="IVN13_000080"
     CDS             77623..77862
                     /locus_tag="IVN13_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010607.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(78225..78485)
                     /locus_tag="IVN13_000081"
     CDS             complement(78225..78485)
                     /locus_tag="IVN13_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010608.1"
                     /translation="MNNETIILGRKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(78671..79168)
                     /locus_tag="IVN13_000082"
     CDS             complement(78671..79168)
                     /locus_tag="IVN13_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP7010609.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(79298..80008)
                     /gene="budA"
                     /locus_tag="IVN13_000083"
     CDS             complement(79298..80008)
                     /gene="budA"
                     /locus_tag="IVN13_000083"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP7010610.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(80021..81685)
                     /gene="alsS"
                     /locus_tag="IVN13_000084"
     CDS             complement(80021..81685)
                     /gene="alsS"
                     /locus_tag="IVN13_000084"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP7010611.1"
                     /translation="MAKKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(81897..82493)
                     /locus_tag="IVN13_000085"
     CDS             complement(81897..82493)
                     /locus_tag="IVN13_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010612.1"
                     /translation="MKIKLYLLSAFFLVFSAGLFFLPDILASSITAKEQRTVDISSLS
                     SASSPSIETAMSQAQSFSIMEESDQSQLDMVNRLREELNQLFQKLSMPKEPVIVQDYE
                     VLISEDSVFWQMEFLTQTGYGTAIIDETNKKVVRLSYFDEDQNNRGKKQIIDDYLNYL
                     PLKADKIEEDGEMITVTLKNGQKLNFLIDETRLLLNYI"
     gene            complement(82483..83865)
                     /locus_tag="IVN13_000086"
     CDS             complement(82483..83865)
                     /locus_tag="IVN13_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7010613.1"
                     /translation="MKYTSKVFFSLLFFFLLIYLVVSGFTLHAIYQRTVSQEKEVLVN
                     QQKNLLLLLSTKTDGDETTDEMRKNGEFVAAAKIGEFEILDKKGKTVFSTKPTLFEKY
                     DSPKELSNHIVEISDMVTVKNKKILYSTSKIPDTTYRLATYHQAKNYEEIAKDSLVYF
                     RLILLFLLLAIIPVTYFLTSMILKPINQLISVAQHITEGKTKERAIVNTQDEIGKLGN
                     SLNNMADKLNYDLETLRQAKENQDLFVSSFSHEIRTPLTSIIGYAQMLKWEKLDQSAE
                     ESVDYILEESNRLKHLSNDILKLIELERTNVSLEEISTSMISNDLNKFLKGMKQGFTY
                     TIELEPANILLDINLFKLLAMNILANSLEAIAEDGKLVVKGIKKEKRYILQFSDNGRG
                     IPAENLSKVTDEFFTGNPSRTTKHLGLGLALVKKITELHNSSFDIESTLDSGTMITID
                     FALGGETDEN"
     gene            complement(83868..84524)
                     /locus_tag="IVN13_000087"
     CDS             complement(83868..84524)
                     /locus_tag="IVN13_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7010614.1"
                     /translation="MKILVVEDNLAINKLLVRTLEKNGYTVYSVFTGKEAADIIEQQE
                     FDLALLDIMLPEIDGYEVFAYMREYDLPVIFLTAKDQISDRVSGLRSGAEDYITKPFD
                     LEELLARVEVVLRRNGKSSSNKLLKWKYIEINEKTRAVTARNQAVSLTPKEYELLLYL
                     IRNKAIILPREKIYQKIWGREEEPNSRTLDLHIQRIRNKLQLGQDLRTIYGVGYLLEG
                     "
     gene            complement(84499..85197)
                     /locus_tag="IVN13_000088"
     CDS             complement(84499..85197)
                     /locus_tag="IVN13_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HAP7010615.1"
                     /translation="MIQKQMYFIRGYYILLLLVLYLSFYEKSVFYLFFTNTILAYIPI
                     ELAMWIKSSRSKLVSLVLILFWLLFFPNNFYLFTDLFHLSLLSPYDPSTMMISMEMEI
                     WYKLLLVVLSVFPTTFLGCESLWIVTDIIVNKIGIGVYQWLVVSIILFINAIGIYFGR
                     FLRLNSTDVFTDSKETLMRLIEAFTRIDSPSFLLIVLIFFFSLIFVYIYKLGIKFALL
                     AEGEDLYEDTSSRR"
     gene            complement(85220..86073)
                     /locus_tag="IVN13_000089"
                     /pseudo
     CDS             complement(85220..86073)
                     /locus_tag="IVN13_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(86090..86980)
                     /locus_tag="IVN13_000090"
     CDS             complement(86090..86980)
                     /locus_tag="IVN13_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7010616.1"
                     /translation="MYKTLKIISIFLLMFIIAAGAYGVSEYMKLQNLSEVSKKTAANI
                     NVKYSADTDTQLPDRFNVLLLGVDTGEFGRTERGRSDSMIAAHVDLKNEAIKLISLER
                     DTYVQIAGQNRFDKLNAAYAYGGEQTAIQTTESMLDTTIPYYMAMNMKGLESILSIVG
                     DIEVVNDFAFSFDHYDFPKGSLTLSPEEVLAWSRMRYDDPNGDYGRQIRQQKVIKAIM
                     QKLLTLESIPKVDKIINVLGNNMKTNLPLGELLKLYITKNNKFKMSTDQLKGEEEIID
                     GISYQKMTDAEIKRISQQLQ"
BASE COUNT        25035 a        17991 c        15087 g        29119 t
ORIGIN      
        1 atttcacagg atttgcggga gtttttgatt agtctttgtt caagtcgcga agcaattcat
       61 cgatatggtt gccatagaca actgattcat caagttcaaa gaacaattca ggtgtcttat
      121 aaatacttag attgtgccct aactctcgac gaatcaatcc tgatgcttta ttcaatccat
      181 cttgtgctgt tttcttatct gatgctaagt cagataaaat gctgtaatag atcgttgctt
      241 gctgtaagtc ccccgtaact ttcacatctg tgatcgttac gttctccaca cgagggtcac
      301 ggaccttttt gcgcaaaata tcattgactt cttttaagat ttcttgaccg actcggcggt
      361 cacggtagtt agccataatg attggcctcc ttttatcagt ttttagtcat ttttgacttc
      421 ttccatgata aatccttcaa ttacgtcatc tactttgatg tcattgaatt tttcaatcat
      481 tgcaccacat tcataaccca ttttgacttc tttcacgtca tctttgaatc gtttcagact
      541 tgcaagttgg ccttcataga ttacgattcc gtcacggatt acacggacac cgctatctct
      601 acgaatatat ccttctgtta cataggcacc tgcaatcgta ccaactttag aaactttgaa
      661 ggtttcacga acgatcattt gaccggtgat tttttcttca aattcagggt ctagcatccc
      721 tttcatcgca gtttcgattt cttcgatggc tttatagatg atacggtgaa gacgaatatc
      781 gacttcttca gtatcagctt gcacttttgc ctgcggtgta ggacgaacgt tgaaaccgat
      841 gataatagcg ttgctggctg ctgctaaggt tacatcactt tcattgattg ctccgacagc
      901 tgaatggacg atttttacac ggacaccttc cacatcaatc ttcttcaatg atgcagccag
      961 tgcttctgct gaaccttgta catctgcttt gatgattacg ttaacttctt tcaattcgcc
     1021 ttctttcagg ctttcaaata agttatccaa agtcacacgg cttgtggcag cacggttttc
     1081 taataatgca cgtttcgcgc gttcttcacc cgctgcacgt gctgtctttt cgtcttcgaa
     1141 gactacgaaa cggtcacctg cttgaggtac atcattcaag ccagtgattt ctactggcgt
     1201 tgcaggtcct gcttctttat cgcgacggcc aagatcgttt gtcattacac ggacacgtcc
     1261 gtaagtatta ccgacaacga ttgggtcacc aacatgaagt gttccttctt ggacaagtaa
     1321 ggttgacaca ggacctttac ctttatcaag acgtgcttcg atcactgttc cgattgcgcg
     1381 ttgtgttgga tctgctttta aatcttctac ttctgcgact aacaggatca tttccagcaa
     1441 ttcttcgatg ttttggccga atttcgctga gatctcaaca aagatcgttt ctccacccca
     1501 tgcttcagga attaactcat actcacttaa ttcttgcata acatgttgag ggtttgcacc
     1561 tggtttatca attttattta cagcgacgat gattggaacg cctgcagctt tggcatggtt
     1621 gattgcttca actgtttgtg gcatgacgcc atcatctgca gcaacaacta agatcgtgat
     1681 atctgtgata cttgctccac gtgcacgcat acttgtaaag gccgcatgtc ctggtgtatc
     1741 caagaaagtg attggtttac catcaatatc gatctggtag gcaccgatat gctgtgtgat
     1801 tccgccggct tcgccgcttg ttacacgtga atggcgtaat gtatccaata aagttgtttt
     1861 cccatggtct acgtgtccca taatcgttac gactggcgga cgtgagacta atttatctgg
     1921 atcgacttct tctgcctcga agaatttatc gatatcagca atgtcaactt ggactttttc
     1981 ttgtggttcc atgccatagt ccgtcgctaa cagttcaatc gtatctttat ccagtgcttg
     2041 gttttgattg accatgacac caagcataaa taatttttta atgatttctg ctggctcacg
     2101 atgaattttt ttcgcaatat ctgctacgtt cataccttca gtatactcta atacatctgg
     2161 caattcacgg aatttgcgtg gtggtacagc tggtttagtt gattgttgct gtttgccttt
     2221 tttacctttt ttattataac gattacggtt attattatta aatttgccgc gattgttatt
     2281 gtttggtttg ttgttattgt tagtacggtt ttgattgccg ccttggctcg ttccttgacg
     2341 attttggctg ctggtatttc ccgtattatt ttggtttgtt gttggttttt ttccttgatt
     2401 cacttgaccg ctcccttgac cgcgatcttg ataactgcgg ttatttttat ttttttgttg
     2461 attgttcgtt gtttcttttt tacgttgatc gttcggctgt tgattggctg gttttctgct
     2521 tgcttgttgt gcagcttgtt gtttcgtgct tgggtttttg aacgcttgtt gcaatttctt
     2581 ctcatcattg ctagtaatcg cacccatatg gtttttcaca tcgatgccaa gttgttgtgc
     2641 tttttccacg acgtctttac tagatttttt gagttctttt gctaattcat agattctttt
     2701 gttgcccatg caatcacctt cctaaccttc aattagctcc ttggtcttct tcgcaaatcc
     2761 ggcatccatt attccgaata ccttgcgagg ttttccgatc atctgcgtga tttctgcttc
     2821 agaaaatagc tcaaagcaag gaacttcata atatgaactc ttatctttta tcttttttct
     2881 tgtattctcg cttgcatcgg ttgccacaaa caccaatttt gctttttgtt tgcggatttc
     2941 agcaatggtc agttcttcac ccgtgattag ttttcccgcg cgcatcgcta gaccgatgag
     3001 attcatggct ttttgccgat taactgtatt cattttccaa ataactcttt acgtgctttt
     3061 tggtgagtga catagtcaag aagttcttga taaaactcat ctgttaatgt tgtttcgagt
     3121 actcgatcga gaattttttt atcccaagct tgttgtactt cttccggctc aagtgagaca
     3181 tacgctcctc gtccaggcat ttttccagtt ggatcgatcg atacttcccc ttcttttgaa
     3241 cgggtgattc ggaccatctc ttttttaggt ttcatttcac ctgaaaccac ggatttgcgt
     3301 aaaggaattt tcctttgttt catctctttg cctccttact aaagttaagc ttctggtttt
     3361 tcttcgaccg tttcattgtt gaatgcaatt gtttcatatt catcgtctgt taaatctgac
     3421 tgaacgattg cttcatcatg cagttcttct gtttcttttt gttgttcttg attttcataa
     3481 aattccgtca tgtcagattc tgatttgata tcgattttat ggttagtcaa tttagcagct
     3541 aaacgtgcgt tttgtccgcg tttaccgatt gctagcgata attggtagtc tggaacgaca
     3601 actgtgcagg cctttggatt tgtctcatcg aagatcacat ccatgacttg tgcaggattc
     3661 aatgcatttg caatgaatac agctggatct tcattccatt cgacgatatc catgttttcg
     3721 ccttttaatt cattgacgat tgcttgaact cgttgtccct ttggcccgac acatgtaccg
     3781 actgcatcga tatttggatc atttgagcga actgccactt ttgaacgatc gccagcttca
     3841 cgggcgatgc tgacaatttc aacgattcca tcatagactt ctggtacttc ttgctcaaat
     3901 aagcggcgca ataggtctgg atggctgcgg ctaacaaata cttgcggacc tttagacgta
     3961 ttttctactc gggaaacata aactttgatt cgatcatgtg gttgataaaa ttcatttggc
     4021 atttgatctt gtttagaaag aactgcctca attttaccaa gattgacata aatataacgt
     4081 ttatcctggc gttcgacgat tccttgcatg atgtcttttt cataagcact gaattcgtta
     4141 taaataatcg ttctttctgc ttcacgtaca cgctgtaaaa taacttgttt agccgtttgc
     4201 gcagcgatcc gcccaaaatc ttttggtgtt acttcaaaac ggatcgtatc accgatctca
     4261 tatgctggat tgattaaaag tgcatctttc aatgagactt ccaactgtga atccatcact
     4321 tcttccgtta cttcttttac cgcatagaca tggatctttc cttttttctg atcaaattcc
     4381 acttcgacgt tttgtgcttg gccatagtga cgtttgtatg cagaaaccaa tgctgcttct
     4441 aaagcatcga tcacgatttc ttttgagatc cctttttcag cttctaaagc gtctaacgca
     4501 tttaacattt ctttactcat tttttctgat tcctctccgt gattaaaatt ggatagctaa
     4561 gcgagctttt gcaatatttt ttcggtcaaa agtcaattct ttttctcgtg tttttatgcg
     4621 tattttcaat gtaagctgtt cagcatcaaa tgattgaaga aatccttcat attgtttttc
     4681 gccatctact ggttggtata aggagacatg gacataatca ccaagtgcct tttcatagtc
     4741 gctttctttt ttcaatggcc gctctgctcc cggggaggat acctccagga aataagcttg
     4801 aggaatcgga tctggatctg ctgcatcaag tttttcactc agtttctcac tgacaaaggc
     4861 gcattcttcg atatcgatac cgccttcttt gtcaataaat actcgtaaga accagctttt
     4921 tccttctttt acaaattcga cttcgacaag ttcaaaattt tgttcatcta aaatcggtgt
     4981 caccatttct gtgactgttt caacgacgct actcaaaagt ttcgcctcct taaaaaatta
     5041 agtgcgtaat tccattaaaa agagtgagcg aaaaatccgc tcactcacag ttagtatcat
     5101 tacctataag aactatacca tactctgctt aaaattacaa gcagacagga aaacagatgc
     5161 ttatagcata tcgaataacg ataattgatt ttcatctggt agatctttca atacgccatt
     5221 gtcgttcata tattcgatca atgtttttga aactttaccg cgggtggcaa gatcttcttt
     5281 ggataaaaat ggaccgtctt ttcttgcttc aacgatttgt tttgccacgt tggttcccaa
     5341 acttgggaca gctctaaatg gtgcgatcaa cgtatcacct tcaataacaa agttaacggc
     5401 atccgattta tacaaatcaa tcattccaaa tttgaatcct cgttccagca tttcattggc
     5461 taactccagc actgtcagtt gattcttttc ttttacggaa gcatctaatc ctttgtccgt
     5521 gatttctttc attgcttgtt tcacagcatc ttttcctttg cacatggaga ctagatcaaa
     5581 gtcgtctgca cgtacagaaa agtatgcaca gtaatacaaa atagggaaat aaaccttgaa
     5641 ataagcgaca cgaagagcca tcaatacgta agctgcagca tgggctttcg gaaacatata
     5701 tttgattttt gaacaagaat cgatatacca gtcgggcaca ttattttctt tcatcgccga
     5761 tagataggtt tctcgcaatt catcagggat cttgttccat tgtcctttac gaacagtttc
     5821 catgatttta aatgccatcc cgctatctaa tccagcatgg atcaaataaa ccataatatc
     5881 atcacgacag ccgattactt ccgccagtgt cgcatctcct cgtcgaatca attcttccgc
     5941 atttcctaac catacgtctg taccatgaga tagtccggaa atttgcagta gttctgcaaa
     6001 agttgtagga tgcgtttctt ctaacatccc tcgaacaaaa cgagtgccga attctggaat
     6061 acctaatgtc cctgttttgg aataaatttg ttcctgactt actccaagta cttcaggacc
     6121 ggcaaatatc cgcatcactt ccgggtcatc cgtagggatc gtttgtgggt cgatccctga
     6181 taaatcttga agcatccgaa taacagttgg gtcatcatgc cctagaatat ctaatttcaa
     6241 tacattatcg tgaatcgagt ggaaatcaaa atgagtcgtt ttccattctg aattttgatc
     6301 gtctgctgga tattggatcg gcgtaaaatc atagacatcc atataatcgg gaataacgat
     6361 gatcccccct ggatgctggc cggttgtccg tttgactcca gtagcgcctt ttgctaatct
     6421 gtcgacttct gcacttcgaa actgcagatt attgtccctt tcgtatcctt ttacatagcc
     6481 atatgctgtt ttgtcggcga ctgtaccaat cgttccggca cggtaaacat agtcttcgcc
     6541 aaacaatact ttcgtatagt tatgggcttc tgcttgataa tcacctgaga agttcaaatc
     6601 aatatcgggc actttgtctc catggaatcc taaaaatgtc tcaaatggaa tatcatgacc
     6661 gtctttgttt aagcgtgcac cacattttgg acattgtttt tctggcatgt caaatccaga
     6721 accatacgta ccatcttcaa aaaactcgga atattggcac tctggacaat aataatgagg
     6781 ggctaatgga ttaacttcag tgattcctgt catcgtcgca acaaagctag agccaacaga
     6841 gccacgtgaa cctactagat agccatcttc attactttta tgcactagtt tttgggcgat
     6901 cagataaata accgagaagc cattaccatt aatagaattc aactcttttt ctaatcgttt
     6961 ttgaatgatc tcaggtaacg gatctccgta catttgtttt gcttttgtgt aactcaattc
     7021 tgatatttca tcttctgaac ctggaatctt cggtgtataa agttcatctt tgactggaat
     7081 cacttcttcg cagatatctg cgattttatt cgtattttct actacgatct ctttcgctgt
     7141 ttcttcccct aaaaagtgaa atgcagttag catctcatcg gttgttcgga aatggacatc
     7201 cggcaaacta tgacgattca acggattcgc tccgcccata gaattgatca aaatttttcg
     7261 gtaaatggca tcttcttcgt tcagataatg tacattacct gttgcaacaa cgattttatc
     7321 caaacttttt ccgatttcta ccaaattttg gataatttct tccaaatcat gttcattttt
     7381 taccaattct tgctcgatca aaggcgcata gaccgctttt ggcataactt caatataatc
     7441 atagaatttt gctcggtttc ttgcctcttc tacccctttt tgcatcatgg cttcgaatat
     7501 ttctccctta tcacaagctg agccgatcaa tagattttca cgcatctttt tcaattggga
     7561 gcgtggtatt cgtgggacgc gttcgaagta ttcgacattc gacattgaaa tcagtttgaa
     7621 caaatctttc agtcctgcct gattttttgc taaaatcgtt acatggaaag gtcgagccct
     7681 tttgtaagaa tctccttctc cgatatgctc atttagctga tcatgataaa gcatgttatg
     7741 attgtccatt gcctctttca caaagatcca tgctaagtga ccagttgctt cagcatcata
     7801 gattgctcgg tgatgctgtt ccaagctaac accaaacttt ttagataaca cacctaatcc
     7861 gaaacgtttg aattgaggat acagatatct agctaattcc aacgtatcaa tgactggatt
     7921 ggccgcttca ggaataccat accttgcgta gctagtattc aagaacccca tatcaaaagc
     7981 cgcgttgtgg gctaccaaaa ttgtatcttt tgaaaattca aggaacatcc gcaatacttc
     8041 ttcttccgac tttgaccctc gaaccatttc atccgtgata ccagtcagat ctaccgttgt
     8101 tcgtgacaat ggatgtcctg gatcgataaa ttcatcaaag ctttcgatca catttccttt
     8161 atacattttg actgcggcta gctcgatgat cgtgtcatat actgcagaca atcctgtcgt
     8221 ttccacgtca aatacgacgt atgtggcttc actcaatgct tcatgtgcat cattataagc
     8281 aattgggaca ccatcatcta cgacattcgc ttccacgccg tataagattt tcacacctgc
     8341 tttttttcca gcactatgtg cttccggaaa ggcttgcgca ccaccatggt ctgtaatggc
     8401 gatagctcgg tgtccccatt ttcctgcttg tgccacaaga tcagagatgc tgtttgttgc
     8461 atccatcgta ctcatattgc tatgaacatg aagctctact cgtttttccc cttcaggagc
     8521 ataatctttg cgaggtgtat gtttgacttc tatgatatct tgcgcattca tgaccaaatc
     8581 acggacaaac gtatcttctt gaatacttcc tcgtaccttt aaccagctac cgacactgat
     8641 cgcatcaaat acttgttcat ctttttcgcc gttcgagaat tttttgacga taaatgatga
     8701 agtataatct gtgattttca aagtcaagat cttgcgtttt gaacgtagtt ctctgacttc
     8761 tttgtcgaat acgtacccct caagtgtgac tcttcgttct tcttcaacga tactgatcat
     8821 tggtgtcgtt ggttcatcgg ctggaatatt tcgtcctagc tggatgggtc cttcaagagc
     8881 tggcgattgc tcttttcttt cctttttctt ttgttcgtgg acaactagac tttcagctgc
     8941 ttgcttcata aaggcttctg cctgttcttg tttgcgttct tcaaaaagtt tcaatacacg
     9001 ttccgcttgc tgctcatcca cttctggttc gatacgaaat tttgggaatc cgtaactgac
     9061 gaacaactct tctactaatg gcaagtattg ttgcttcaaa taagaaatag ctcctgtact
     9121 gtcgattggc aagatgactt tacgatcttt tatcacaggt acctgcgttt tcaatacttg
     9181 ctgtgctagt ggtgtatcgc attgctgatt ttccaaagct tgggcccagt aatcttgcaa
     9241 gagctgttca tcgaatgtct ggtcatctgc taagatattc agtttgactt gtgcaatatc
     9301 tttaaaagcg atagtcagat gctgagtcaa tgaccgatat agcataatag gcaaaatttt
     9361 ggaaaaatgt agcgtaaatt cccataaacg cgatttacga tggaccacta cactatccat
     9421 ttcaccagca gtaagcagtg gatgattttt ttctgcttcc tctagttgga tttgttccaa
     9481 cagtttttca aataaatctc gtttttctga caaagatata cgccctttct gatgagaaac
     9541 aatgcaattg atcgcatctt atcctcttct ttttcagtac cttttagtat actcgttttt
     9601 tctgagtttt acattaaaaa attggaactg cgattcaaat cgcagttcca attttaaaag
     9661 cgaatcatca gattgtcaaa aacagctgat tcagctattt ttattttctg tgttttaacg
     9721 cttattattc gccttggttc aagaggattg gtaaagtatt cgccaattct tctttacgta
     9781 cttcaaccat ttcgccagtt ttcttgattt tcacttctac gattccatcc actgcttttt
     9841 taccgacagt gattctgatt ggacagccaa tcaaatcaga atccgcgaat ttcactcctg
     9901 cgcgttcatt tctgtcatca accagtactt catagccagc ttttgtcatc gcttcttcta
     9961 gttcattcgt caggcttgtt tggtattcgt cttttagatt catttggacg atatgcagat
    10021 caaatggcgc aattcctttt ggccaattga ttccttgttc atctgaatgt tgttctacga
    10081 ttgctgataa taaacgactt acaccgatac cataacagcc cataatcaca aatttttctc
    10141 gtccattttc gtctagtaca gttgctccca tagattcact gtaacgagtg ccgattttga
    10201 atatatgtcc gatttcgatt cctcgagtaa aggcaagaac accgttgttg tctggtgaag
    10261 gatctccttc ttgtacaaaa cgaagatctt cataagaaag cacagtgaaa tcgcgttctg
    10321 gattcacatt tttatagtgg aatcctgttt cgttcgctcc agtaatggca ttcgctaaat
    10381 cttggacata acggtctgca tatactttta cgtcatctga aacaccgact ggtccgacag
    10441 aaccaaactc agcacctaga tatttttgtg cttcttcttc tgttgcttct tctaaaaagt
    10501 cagcagataa aaagtttttc agttttacgt cattgacttc atggtctcca cgtaccaaaa
    10561 caaggacagg ttgttcatcc gcaatgaaca atacagactt gatgattttt tgaggttcaa
    10621 cagaaaagaa atctgctact tcgtcaattg attttacgtt aggtgtagct actttctcca
    10681 attcaagctg tgtttcatgc gatttttttg atacatagta gcttgtagcc atctccaagt
    10741 ttgcagcata atcactttca gtcgaataac agatcgtatc ctccccgatt tctgagatcg
    10801 ccatgaattc ctttgagtct ttcccgccca ttgcgccgcc gtctccgatg atcgagcgaa
    10861 attctaagcc acaacgcttg aatacttcgg tataggcttt ttcgtaatct ttatatgtct
    10921 cgtccaaact ttcttccgtt gcatggaaag aatatgcatc tttcatgata aactctcttc
    10981 cgcgaagcaa accgaagcga ggtcgttttt cgtcacgata tttaggttgg atttgataaa
    11041 gatttaaagg taatttctta tatgaattga tttcattacg catcaaatca gtaaaagttt
    11101 cttcgtgagt tggtccaagg atcatttttc gatcattgcg gtctttgaaa cgatacaagt
    11161 ttggtccata tgtttcgtaa cgcccggatt cttcccaaag ttctgctggc aagatggcag
    11221 gcatcagcat ttctaccgct ccgatttttt caaattcttc ccgcataatg cgtttcagtt
    11281 tttccaatac acgatttgct aatggcagat atgaataaat acccgctgat acttggcgga
    11341 tatatccagc tcttagcaag atttgatggc ttaatacttc tgcatcattt ggaacttcgc
    11401 gtaatgttgg aatcaacatt tttgattgtc tcattctata tactccttta tttcctcaaa
    11461 atagttatcg tttgttatta taccctattt gtcaactttt tctagaaaaa gaatcgttga
    11521 atatcgttcc atgtaactag caccatcaac aacattaaaa atccaaagcc agctaatgtc
    11581 aatattcctt ctttttcttg acttaatggt ttcccgcgaa tcccttcaaa aatattcaag
    11641 actaatttcc cgccatctaa ggcaggaatc ggaagcagat tgacgattcc gagattcatt
    11701 gaaagaagcg ccatcaaact aataacagtt gttacacctt gatttgctgc ttctgaagac
    11761 aattggtaca tcatgacagg tccgcctaat ttatctaaac tgaaacctgt aaatagtgat
    11821 cctaaggctt tgaaaatttc caatgaaccg ctgaaagctt gacgcgtccc tccaatgatc
    11881 ttatccataa aacctgtatt cattggggct tttataccaa gttggccaac tttttcgcca
    11941 tttgactcta cagatttcgg tgtcacgtcg acagattgca cctgaccttc tcgttcgatc
    12001 ttaaagtcta atgttttacc tggatttttc gtaatgactg ttgtcagatc attccatgaa
    12061 tggatttctt ttccgtccac actgactacc tcatcatttt cttttaagcc tgcttctgcc
    12121 gcagctccat taggcatgat ctctcctacg cgattagtgt tcgtcacttg aacaccacct
    12181 tgcataaatg ccagaatgat gaataacacg attgccaaaa taaagttatt catgggtcca
    12241 gcaaagttgg tcagcatacg ctgccacaat ttagccgatt ggaactgtac gtcttttggc
    12301 gcaattctga tttctgttcc gtcgttttca ataatcgtcg catcgtgatc aacagggtag
    12361 gttactactt ctgtttcgtc tccattcaca taaccagtga tagtcagctc atcttctaaa
    12421 tcataacggc tcaattccat cgggatagca tttgtcaatt ggattttctt acttaaattt
    12481 atcttctcta cgatgccgtc agaatttagt aaaagagaaa gaggcattcc tggtgccatt
    12541 tctgtttcat catccccatt gcctgccatt cgaacataac cgccaatagg taataggcga
    12601 agcgtatatg ttgtcccgtc ttttgcttga tgcccataaa ttttcggacc cataccgata
    12661 gcaaattcgc ggacgaggat tcctgatcgt ttcgcaaaga agaaatgacc aaactcatga
    12721 acaatcacta atataccaaa aacgatgata aatgtcagaa tcgttttcat agcttttttc
    12781 tcctttactt gttttttgtg ttttttataa aacaacgata aaacactata attttaccat
    12841 agtataccaa agacaccaat agtatcaata taatgaaaaa taaaaacctg cgtcttttat
    12901 gaaattcaca aagaaacgca ggtatactta ctaaataatg ccaaataaat gcatgatagg
    12961 aaatacaaac agcaaactgt cgaaacggtc taagattcca ccatggcctg gtaagatatt
    13021 tcccgagtct ttcacatcat aatgacgttt gattgcagat tcgaccaaat caccaaattg
    13081 tccgactgct gaaaaaataa tcgtcatcag aagcatgaca aacataccat gattgaatag
    13141 ttcttttgct gggtatagca ataaataaag aaatgcgact acaacagcac taccgatacc
    13201 accgagcgca ccttcaatcg ttttgttggg cgaaatatca ggacttaact tatgctgacc
    13261 ataacgtcga ccaaccatat atgctccgat atcagttgcc caaacaataa atagaccaaa
    13321 tatcaaaaca gccaagcttt ctgttcgtgc attgacaaaa ttctggaaac ctattcccac
    13381 atacaagctg acaagcacag gaaaacctgc ttcatcaatc gtatacgtat ttttagaaat
    13441 aactgatgtt cctaaaagaa tcataactat aagataaaat aacataaatg tacttacatt
    13501 ctctggaaga aagaaaaacc atctttcttt tggcaaaaca agtaacactg caccaacggc
    13561 ggataaaact ccttcaaaac ttaagagtgt caatcctttc atccggaaca gctcgtaaac
    13621 tcctactacc gctaagattg ctgctgtaag ctcaaccgct atgcctccga tccatataat
    13681 cggaataaac aagactagcg ccacagctgc tgtgatcaca cgttgtttca tgactgatcc
    13741 cctttctcat cttctgtttt ttttacaccg ccgaatcgac ggtctcgatt ttgataagag
    13801 gcgattgctt cttcaagatg cgcaccatca aaatcaggcc aaagcgcttt tgtaaaatac
    13861 agctcactat atgcgatctg ccataacaga aaattactaa ttcgctcttc tccacttgta
    13921 cggatcatca gctcaggatc ttgtaactct ttaggcaaga aacctgtcat taaatgctta
    13981 gcaatcaatt catcatcgat ttcttctgcg tgtatttctc cttttgctac ttcttcggca
    14041 atttcttgga cagcagtcac gatctctgct ctacttccat aattcaaagc aaaattcaat
    14101 accattccag tattgttttt tgtttgctca attgcacgtc ttacagcatc ttgcgtatgt
    14161 tctggcaata cgttttcata acccatgaca tgaactttga cattttcttt aatcaattct
    14221 ggtacgaacg tatcaaagaa gtcgacaggt agttgcatga gaaaacttac ttcatctttt
    14281 ggtcgcttcc agttttcagt cgaaaaggca taaagcgtca acactttcac tccaagcctt
    14341 gatgcttttt ttgtgacttt ctttactgtt tccatgcctt ctttatggcc agcaactcgt
    14401 ggcaaacgac ggttttgcgc ccaacgacca ttgccgtcca tgatgatggc aatgtgctga
    14461 ggaattggtc catcggtatt aaattgaaat tcgctttttt cttgaatata tttgtttttt
    14521 tgcggaaaaa aacgtaacat tctttttcct cctatactta aaagattccc actatcttaa
    14581 gtattatagc aatatctaac tgtttttcct atgaaatcat tgttttttta tttaaaaact
    14641 gaataatttt ttcttttttt aatttttaaa taaaagaaat ataaataaaa acagaggaag
    14701 cggaatgatc ctcccctctg tttttatgat tttattaaac ttccaataat tctttttctt
    14761 tatcagcagc gatcttatct acttctttaa tgctgttatc tgtcaatgtt tgtacgtctt
    14821 tttctaatcc gcgcaaatca tcttcggtaa tatcaccatt tttttgttgt tttttatatt
    14881 catcgatcgc atcacgacgg atgttacgaa tcgcgatttt tgcattttct gcttcttttt
    14941 tcacatcttt agctaattct tttctgcgtt cttctgtcag ttgcgggata actaagcgaa
    15001 tgacatttcc atcattggtt ggactgattc cgatatcact tgccataata gctttttcaa
    15061 catcttgaag aatgctcttg tcaaaaggtg tgatcattaa aacacgtgct tctggaattt
    15121 ggattgatgc catttggttc aatggtgttg gcgcaccgta gtagttcaca gtaatacggt
    15181 ccaaaaggct agcgttcgca cggcctgcgc ggatttgccc aagttcacgt tgaagatttt
    15241 gtgctgcttt tttcattttt tcttttgctt ctgtcataat tgcatctgcc atttttattt
    15301 cccccttaca gttgttccga tattttcgcc taagattgca cggcgaatat ttccgtgttc
    15361 atttaaattg aagacaagca gtggaatatc attatccatg cttagtgaac ttgcagttga
    15421 atccatgact tgtaatcctt tggcaataac ttccagatga gtcagttctt caaattttac
    15481 tgcatttgca tctactttcg gatcagcaga ataaacaccg tctacattgt ttttagccat
    15541 caggataaca tctgcgccga tttctgccgc tcgtaatgct gctgttgtgt ctgtcgagaa
    15601 gtacgggttg ccggtaccgc ctgcaaagat aacgatccgt cctttttcta aatgacgttc
    15661 tgctttacgg cgaatatatg gctctgcgat ttgacgcatt tcaatcgaag tttgcacgcg
    15721 tgtcggtaca cccacgtttt ccaacgtatc ctgtaaagct aaggcattca taactgtagc
    15781 taacataccc atgtaatcag cttgagcacg ttccatgccc atttgtgctc cgatttgtcc
    15841 gcgccagata tttccgccac ccacaacgat tgccatttca acgcccagct cgtgaacttc
    15901 tttgatttct tggatgattt ctttgattac tggtggtttg attccaaatc ctgcatcccc
    15961 agctaaagct tctccactca attttaagac tacacgctga tatttcggtg tgaccatgtt
    16021 cattcctcca atattttcta ttgccatttt accatactta gatgatttcg acactctaag
    16081 actggttttt cttgttttgc ttaaaaaaag ggagcgcaca aaccgtgcgt tcccgtctgt
    16141 tattcaacag aattattttt tcacttggct cataacttct tctacaaagt tatcttcgcg
    16201 tttttcgata ccttcgccaa cttcgaaacg tacgaatgtt ttaacagttg cgccttttga
    16261 tgcaacgtat ttttcaacag tcatatcagg atctttaacg aatggttggt ccaccaaagc
    16321 gatttctgct ttgaatttct tcaagcgtcc ttctaccatt ttttcaacga tattcgctgg
    16381 tttgccttcg ttcaatgctt gttctgtaag aacagttttt tcatgttcta attcagcttc
    16441 tggaatttgt gattcgttta cataacgagg attgatagct gctacgtgca tagctacgtc
    16501 tcttgcaact gtttcgtcag ttgttccatc tagaacagtc aatacggcaa tacgtccgcc
    16561 catatgcaag tatccaccaa atgcagcatt gtcgtctttt tctacaactt caaagcgacg
    16621 gaagctgatt ttttctccga taacttgagt tgcttcgatc aagtcagatt caattgtacc
    16681 tttatctgtt ttgattttca ttgcagcttc catgtctgct ggtttgtttt cagcaacaag
    16741 ttctgcaatt tcttttacta aatcttggaa catttcgttt ttagaaacaa agtctgtttc
    16801 tgagtttact tcaacgattg ctgcagtgtt acctttcaca gcaactgaag ccaatccttc
    16861 agctgcaatg cggtcatttt tcttcgcagc ctttgccatt cctttttcac gtaaaagatc
    16921 aactgctttt tccatgtcgc cttctacttc aactaatgct tttttcgcat ccatcattcc
    16981 tacgccagtc atttcgcgta gttcttttac cattttagct gtaacgtctg ccatgtttaa
    17041 ctcctcctgt gtgtttttct ttttaaaaaa agctgcttca aaggagagtg aggcttgtcg
    17101 cccagccttt gaaacagctc catcattcaa aattattctg ctgaagaatt gtcgccttca
    17161 acaacatcta cgatttcttc gattgatgtt gctgtgtttt cttctgtgaa atcttcttca
    17221 actacttgat cttcaccttg gtttccttcg atgaaagcat cagccatttt tgaagtaatc
    17281 aatttaacgg cacgaattgc atcgtcgttt gaagggatta ctacgtcgat ttcatctgga
    17341 tcgcagtttg tatcaaccat agctacgatt gggatattta atttgtgtgc ttcttgaaca
    17401 gcaatacgtt ctttacgtgg gtcaacgatg tacattacat ctgggattct tggcatatca
    17461 gcgataccgc ctaagaattt ttcaagacgt tcacgttctt tgttcaaacc agcaacttct
    17521 tttttaggta gaacttcaaa agttccatct tcttccattg cattgatttg tttcaaacgg
    17581 ctgatacgtt tttggatagt atcccagttt gtcaatgttc cgcctaacca acgatggtta
    17641 acgaagtatt gtcctgcacg agttgcttct tctttgattg cttcttgtgc ttgttttttt
    17701 gtacctacga ataatgctac gccgccttct tcagcaacgc tcttcatgta atcataagct
    17761 gcatctacta atttaactgt tttttgcaag tcaatgatgt agattccgtt tctttctgtg
    17821 aagatatatt tcttcatttt tggattccag cgacgagttt ggtgaccaaa gtgtacgccg
    17881 gcttctagta attgtttcat agaaattact gccatgtttg tttcctccga tttggtttta
    17941 tttttcctct tcttatcttc taccttgccg gcaaactttt tcaagcacct accgacaatc
    18001 caataagaat gtggatttgc tgatgcataa agcaccctca gctattatac agacaaaaga
    18061 tccgtatcgc aagagcaaat atacttttct gctcaacatt caatagttta cctgaaagat
    18121 aaaaagaatt caagaaaaac agtagatttc tctttgtttc tttattatcc tttttccata
    18181 aaataatttt aggtataaaa gacgaatatt attgggcaat ccttcttttt tttgttatga
    18241 tacatgtgaa atctttcgta aaggagaaag aaaatgcgca aagctcaaag acatttgctg
    18301 attaaacaag tcattgaaga attcccgatc cgtacgcaag aagaactatt aaacaaactt
    18361 gggcattatg gcgttaccgc cacgcaggca acgatttctc gagatatccg tgatctaaaa
    18421 atcgtgaagg cacctgatga aaatggcgtt tcccgcttcg tattgtttcg cgaaaaagga
    18481 actcctgaga ctaggactga ggaagaggat cggctggttc aaatgattga agacattgta
    18541 ttgaaagtcg aacgagtcca ctttttgacg atcgtcaata ccttgccaga caatgctcat
    18601 ttatttgcat ctgttttaga tgagatcaaa cctccctcta ttgtcagtac aattgctgga
    18661 ttcgatacga tcatggtcat ctcggaaaat gaggaggacg caaaacgagt agaagaattt
    18721 cttcaccatc caactgaagt atcacttttt taatcagagc acgtattcct aatatggaat
    18781 gcgtgttttt ttattttcaa tattttcctt attaaataaa cctttacact ctttacaaaa
    18841 atacatcatt ttttattcaa ttttttcaac accttgattg aatcacaata atcatcattt
    18901 atattggatg ttaacaagag cagcttcttg ctctttgtgt atttcaaaaa agaaatgaaa
    18961 ggaaatcata tgtataaatt attaactcct ggaccattga cgacttctga aacagtgaaa
    19021 aatgctatgc tgatcgatca ttgtacttgg gatcaagaat acaaagaaat tacacaatgg
    19081 ataagaaaaa aattattgga tcttgctcaa gtatcggaag gaatatacac aacggtctta
    19141 atgcaaggta gcggctcctt tggggtagaa tcggtattaa gttctgtgat caggccagaa
    19201 gatactgtac tgatttgttc aaacggcgca tatggacaac gaatgatcaa catgtgcgaa
    19261 tgtctgaaac tgaactatac cgtttatgag gtatcggaac atcaaatcct ccaagctcat
    19321 gtgattacac agctgattga agagaatccc tctatctccg cactagcaat ggtccatagc
    19381 gagactactt ccggtatttt gaataatttg gaagagattg ctgctgtcac acaagaaaaa
    19441 gatcttttgt ttatagtgga cgcgatgtca agctttggtg ggataccgat tcgtgtagcc
    19501 gaactaggga ttgatttttt gatttccagc tcaaacaaat gtattcaagg tgttccaggc
    19561 ttttcttttg tacttgctaa taaagcggaa ctagaaaagc gaaaaaatgt gagtcgaagt
    19621 ttgtcacttg atctatatga ccagtaccgc gtgatggaaa acgagaatgg caaatggcgt
    19681 ttcacttccc ctacccatac ggttttggct tttcaacaag cgctgaagga actagaggca
    19741 gaaggaggaa tcgaaactcg atattcacgt tatctcgcca ataatcatgt actacgacaa
    19801 aaaatgacag agatggggtt ccagcctttc attcaagaat atcaggggcc attcatcacc
    19861 tcttttcttt accctaagga ctggcgattt gattttaatc atttttatca gtatttaaag
    19921 caaaacggat ttgtgatcta tcccgggaaa atcagcgatc atgaatgttt tcgtatcgga
    19981 aatattggcg agatctatcc ggaagatatt ttggaactga cagcaattat caaagactat
    20041 aaggagaacg ttaatgatta aaactgtaat ttttgattgg gcaggaacaa ctgtggactt
    20101 tggctgtatg gctcctgtcc acgcttttag aaatgctttt ctagaaaaag gaatccaact
    20161 gactgacaaa gaaatccgcg aaccgatggg aaaattaaaa tgggatcata tccaacagct
    20221 tctttccctc ccttctgtca aaaaacaatg gatagctatt tatggacaac tacctcagga
    20281 aaaagaggtg gaggaattgt atcaacaatt tgaacattat ctgtttaaag atttggtcaa
    20341 gcattctacc ttgaaacccg acacattgga aacgatcgaa cggttgagaa acgctgggat
    20401 caatattggt tccacgactg gatttactgc tcctatgatg acaatcgtag cagaaaccgc
    20461 aaaaaaagaa gggtattcac cagacttcat ggcaacttct gaagacgtcg aagggtatgg
    20521 tcgaccgttt ccttatatga tccataaaaa catgcagtac ttcaaaaaca agtcggttga
    20581 tgaagtaatc aaagtcggag atacggtctc agatatccag gaagggaaaa atgccggagt
    20641 gttgacagta ggtgtaattg aaggtagttc gctaatgggg atttcttatg atgagtacca
    20701 aaattagaga tggggaaaaa agatcgttta aaacagtctg ttgctcttag attccaacaa
    20761 gctggcgctg atatttgtgt aaacaattta aaagagttaa cagaatacat tttgtacgct
    20821 gacaagaatc cagaatgact tttatacaaa atcagaggtc gtgaaaaagc tgtactgcat
    20881 caagtaataa gaacagccaa acaaaaatag ctcctcatat ttttcgtttg gttaggctta
    20941 ttaccgcaga tgcaagcttt tgaacaccgt ttattcgaaa aaaaaaggga gctgtgacac
    21001 gacttttgtc acagtcccct tatttacaaa caaacagtgt ttaagagaca acctctccac
    21061 taaacggtgt tcattcagct gactctcggt atttgctcaa aaaacgataa aatttgaaaa
    21121 gctattttct cttttgtttc tctaatactc ggatcagaac gctgatttca cattctcttt
    21181 ttcttacatc ttatctggtg catgaagtcc tagcaaacga aggtcttctt tcaataatac
    21241 agtcactgca taaactagag ctaagcgtgc ttctttttgt tcatcatcag ctaaaatctt
    21301 cgtatgagca tagtatttgt tgaatgcttg tgctaatttg attgcatgtt tcgcaatcac
    21361 tgacggttcg tatttttccc cagcagacaa gaccgtttca gggtattttt gaactaattt
    21421 aaccacttcc cagctggtat cgtcattcaa tgcatagtct gctgtttctg aagggacaaa
    21481 tcctgctttt tccaacaggc tgactgcacg agcatgtgta tattgcacat aaggtccagt
    21541 ttctccttca aatcgaacaa cttcttccag gttaaagtca aatgtattca gtcgatcgtt
    21601 tttcaaatcg tggaaaatga ctgccccaac cccaacttgt ttagctacag catctttgtt
    21661 ttccaaatca gggtttttct cgctgatttg ttctttagca gattggattg cttcatttaa
    21721 tacttcttct aataagacga tctttccttt acgagttgat aattttttac caccttgcgt
    21781 gatcaaacca aatggaatat ggcgcatatc gtcagaccag tcaaagccca tttccttcaa
    21841 gacagctttc aactgtttaa aatgatagct ttgttcattc cctactacat atagagattg
    21901 tttgaagtcg taagtacgtt tacggtacaa tgcagcagct aagtcacgtg taatgtatag
    21961 tgtcgcaccg tctgacttct taatcaacgc tggattcagg tcataagcag ataggtcaac
    22021 gatctctgca cctttatcct cgttcagcaa atgtttttct tctaaaagtt tgacgatttc
    22081 atccatcttg tcattgtaga aagcttcacc gttcaacgaa tcgaaacgta cttccagcag
    22141 atcatagatt ttattgaatt ctttcatcga ttcatcacgg aaccattgcc ataattgaat
    22201 agcctcttca tcgccttctt ccagacgttt gaaccaagca cgagcttctt catttaattc
    22261 tggttctgtt tcagctactt catggaattg gacatataaa cgaagcaatt cattgattgg
    22321 ttctgcttta acagcttctt ctgtacccca ttttttgtaa gcgacgatca gttttccaaa
    22381 ctgtgttccc cagtcaccta aatggttgat gcggattggc tgataaccga ttttttccat
    22441 aatgaaaccg atcgaattac cgataactgt cgaacgcaaa tgtcccattg agattggttt
    22501 ggcaatatta ggagaagaca tgtcgattgg tactgttcct tgattcccga tattgctatc
    22561 cccataatgt tctttttctt tcactactgt ttgcaaaact tttttgctga taagttcttt
    22621 attcatgaaa aagttcaagt aaggtccgac aacttcgatt ttttcaaagt tggcagaatc
    22681 gattttttct gctaaatctg cagcgatttg ctgtggcgct tttcggtaaa cttttgctaa
    22741 tgaaaaggct ggaaatgcaa catctccgtg ttcagctgat tttggatttt ctagtaattg
    22801 ttcgacttgt tccagtgtta aatcatcttt gacagcgtca tagacagctt ttgcgacaag
    22861 atctttatta ttcatcttta ttcctcctaa agtgctccct tgattgttaa acaaaaaaaa
    22921 tctctagcga cggcatactt agtatgccat cactagagac gagatttccc gcggtaccac
    22981 tccaattgtc ctaaaaaagg accagctcca tctgatatcg gtagacgacc gtctgtttaa
    23041 aacagaattc tccaagtgcg cttcgttgta tcttcttcat tcggcttcca ccacttccga
    23101 attcgctagc caagaatgat acaagtactc tcttgttcaa caaatcttag tcttattaaa
    23161 ctttcttgaa ttatatcaga aaaaaaaacg aaaggctagt cgtttctgat aaaaaaacaa
    23221 tccagatggc ctaaaggaga agaaatacca cctggatagc tataaatcat ttcgtaaata
    23281 cgaaagactt aacaaattaa tcagctacga taatcgttcc ttcttcagaa gctaaaagat
    23341 tctcgatatt ttctaaagaa gtgatgattg ccttagcgtt tggttttgct tcaacaaatg
    23401 caatagctgc ctctactttc ggaagcatgc ttcctggagc aaattgcttt tcatcaatgt
    23461 attgtttcat ttctgaaaca gtaacagttt ctaatttctt ttgatctggt ttttggtaat
    23521 tgacatacac attgtctact cctgtcaata cgatcagcaa atccgcatcg atgatttcgg
    23581 ctaatttttc tgaagcaaaa tctttatcga ttacggcttc tcgtccttct aatccagtag
    23641 ctgtttctac aacaggaatc ccaccgccac caacagagac tgtgataact ccttgatcga
    23701 ccagcgtttc gatgacatgt gcttctttaa tcgagatggg tttaggggaa gcgactactt
    23761 ttctccatcc acgtccagca tcttctttga atgtcacagt ggagtcagca ttcatttgtt
    23821 ctttggcttc ttcttccgtg tagaatggac cgactggttt acttggattt ttgaatgaag
    23881 gatcattttc atccacaata acttgagtga ctaaagaaac cacatcttta tcgatccctt
    23941 tttccttcag tacttcgccc atggcatttt gtaaccagta accgatagat ccttctgtca
    24001 ttgcaacaca tgtatctaaa ggcattgccg gtgttttctc agaatcagca gcttgctgct
    24061 gaatcaatag gttaccgact tgcggaccat ttccgtggga gatgatcaat tcatctcctt
    24121 gttcgataaa tttcactaaa tactttgctg tttccattaa cgcttcttgt tgggcttttg
    24181 cacttgcatc cgtagacaag attgcattac cccctaaagc gacaactact tttcgtttgg
    24241 ccatttcatt cgtcctttct tcagttctac cgaattttct agataacaaa acgagccagc
    24301 gttttgtgat tttccctcaa tgctgactcg ttttttcgtg agatgaatgt ggttatgctt
    24361 aaacgcgagg gataaacaag ttacctaatg ttgctgccat gatcgctttg attgagtgca
    24421 tacgattttc tgcttggtca aattgacgag catatttgct gcggaagact tcatctgtta
    24481 cttccatttc agtgatgcca aagcgttctt tcatttgttc accgtaaaca gtgtttgtat
    24541 catggaaagc tggcaagcag tgcaagaaga tcaaacgatc tgtgttgtgt gttttttcta
    24601 ccatttccat attgatttgg taaggtttca acaatttgat acgttcttcg aatttatctt
    24661 cttcacccat tgatacccat acatcagaat aaaggacgtc tgctccgtct acacctttat
    24721 caacatcgtc ggtaatcatc aattgtgcac cggatttttc agcaaagcct tctgccattt
    24781 tgacgatttc ttcttcaggt tgtaattctt ttggtgcaac gatacgcata ttcgctccta
    24841 aaattgcacc tgtgactaat aaagagttag ccatattgtt tcgaccatct ccgcagtaag
    24901 caacagtgat accttctact gttccaaagt tttcttggat agttaaaaag tcagcgatca
    24961 tttgtgttgg atgccattca tcagtcaagc cattccaaac tggaacacca gagaattctg
    25021 ctagttcctc taccattttt tgactgaatc cacggaattc gattccatca aacatacgac
    25081 ctagtacttt agctgtatct tctgtggatt cttttttacc taattggata tcatttgcac
    25141 ctagatattc aggatgtgca cctagatcga tcgctgcagt agtaaatgca gaacgagtac
    25201 gtgtagatgt tttttcaaat aaaagcgcaa tattttttcc ttctagatag tgatgtggga
    25261 taccacgttt tttcaagtct ttcaagtgtt ctgaaaaatc gatcaaatat tgtaattctt
    25321 cttttgtaaa gtctttttct gctaataagc ttcttccttg gaatactgat tctttcatat
    25381 taaacattct cctttagatt gtttttattt aagattaaag aggaaatccc gacggcagct
    25441 gacagtaaga ccgccgtcag gaaacaaatt acttcaagtc ttcacggaca agtggttggc
    25501 tcatgcagcg tggaccacca cggcctcgtg acaattcact tgaattgatt tctaacactt
    25561 tgatgccata gctacgaagc aattcgttcg atacataatt tcggttataa gtgactacaa
    25621 cacctggagc gattgccaat gtgtttgatc catcattcca ttgttctcga ggtgcaacga
    25681 tctcatctcc attaccagtt gggatcagta caagatcatc taatccaaga gcagctttca
    25741 tcgtttcgtg taagttatct gaatgagtaa tcttgatatc atcaccatct ggttcgattg
    25801 taaagacatc catcttaccg tctttacttt gaattgctgg atggatagtg aatttatcgt
    25861 gatctaccat tgtaaatacg gtatccaaat gcatcattgc acggttgttt ggaattttga
    25921 ttgctaacac tttttcaaat cctgaatttt ttgcgaatag gtggcgtgct aatttttcta
    25981 aagctttcgc attagttcgt tgagaaatcc caacagctac tactttatcg cttaagatca
    26041 attcgtctcc gccttcgata tgatctggat tttcacgatc caaccatact tcgattcctt
    26101 tatttgcaaa tcttggatga tgtttcaaaa tatattctgt aaacatcgat tcacgacgac
    26161 gtgcttcaaa agtcatctta ttgactgtca tcccatttcc cattgatgcg gaagggtcgc
    26221 gtgtaaagta caagtttggc attgggtcca tgtaaaatgg atagtcatcg tctgctgaca
    26281 gatcgtatag actgctagaa cgtacttcaa tatcttttgt acgaacccca gccatgattt
    26341 tatctaccat ttcttgcgtt tccattgata gaaggaactc atgaagaccc tctcttacag
    26401 cattcgatgc aatatgagat tcatccaaca ttttattcaa gaattgttct ttgacattgc
    26461 ctgcgtcaat cgcttctgcc gctaattttt ccaagtaaag agtctcgacg ccttcatttt
    26521 gcaatgtctt tgcaaagttg tcatgttctt cttgagcaat cggaagatat ggaatatcat
    26581 cgaacaacag acgatccata atatctggtg tcaaattttc cacttcttgt ccaggccttt
    26641 tcagtaaaac tgtctttaat ttccctattt cagagaaaac gtgaataggt ttatccatgt
    26701 tgtgttgcct ccttcttttt gattacatgt ataggatatc ctccttttgt aatcactttc
    26761 ataattttgt ctcattcaaa atgagacatt cctcacatta atattcgata attaaataat
    26821 taaacgtttt tctattttta agcgtaattt attcattttt tattctgtat acactttcaa
    26881 aaaaatattt attcattgaa aaaaattttt tgaataaatt aaaaaaataa attatattag
    26941 cgttgttagc gtttactata tgtattattg ttgtttttta tacacaaatc attcgcttca
    27001 aaacaaatta ttcaatacca ttgtatattt tttacataat aacaaattat atttgtaaag
    27061 tattaaaact tgtttctaat gttggtatgt taaaacttta caaattcttt aaaaaaagtg
    27121 acaataaata tagtgaacaa taaggagagg actttttaat gcgtgttaat aaaatcgatt
    27181 ttcacttttc aaaactcaga tatcaacctg attttgaaca tttctcggat gaagagttcc
    27241 aacagatcaa ggaacatact gttcttcgtt cttataaaaa aggacagatc cttttcgacg
    27301 atggtgatcc cagacaacgg ttgtatatct tgaccaaagg actcgttcgt ttagaacgtt
    27361 atgatgaaag tgcaacttat tactactatg actatgtgaa tacaaataac ctttttccta
    27421 tgtcaggaat gtttggagaa gagacctaca cttatacagc tcaagcgatg actgatattg
    27481 aaacattcta ccttcctgtt tcctactatg aaaaattagc acgaaaaaat cccgaacagt
    27541 tgatctatat gatacaaaaa atttctaaag tagttgaaat acatgaattg cgtatccaaa
    27601 tcggattgac ctctagcgcc tttgaccggg taaaacaaaa tttattcatt ttgaaagaag
    27661 aattgggcgt accaacaaaa gatggtcata tcaccattcc ttatccgatc accttaaaag
    27721 aattagcagt gaatagcggc acgacacgtg aaactgctgg acaagtaatc aaacaactac
    27781 gtgattccgg actgttagat tatgacaaaa agcgttttac tttctgtaag acagctacta
    27841 ctccagctat gagagaataa cgcgaacaaa aataagcagt gaccctgata tttatcaggg
    27901 tcactgctta ttttcctccc taaaacttct gaaagatccg gcagttttag atcactgggc
    27961 aagactcaaa gattttctat gtgtgtttca gttatgtaat attcactgtt tgcaaaaaat
    28021 actgatagat taactagagg aagcgtcttg tcctcatccg acctagatta ttcgatattc
    28081 ctcgacaata aacgattaat cagtgagggc aatcagagga acggataatg atgaataagc
    28141 tgtaaaaaat agggctttga caaaagcgtg tcacagccct tatttcgatt aaacggtgtg
    28201 tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg
    28261 aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg
    28321 ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt
    28381 ttcctgggct gttcttatta cttgatgcag ggcaaatttt tcacaacctc tttattcaga
    28441 taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt tttgcatttc
    28501 cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa gagggggaat
    28561 cactagttct acctcaggga gtttagttgc caaagcatcc tgcaatcctt cacgcaaccc
    28621 ttgattggcc gctacacctc cagctacaat cagttgcttg actggataat tttgacaagc
    28681 tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag ctgctaaatc
    28741 atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga taaatgcact
    28801 ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc gtggaaaatg
    28861 gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg ggtaagaaag
    28921 acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc
    28981 aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc ctccgctaac
    29041 aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat agatatgccc
    29101 tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc
    29161 agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag cgactgccga
    29221 taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata aggtgatttg
    29281 ttctacgtga tggcggctag ctacttcagg aactacgcca ccaaaacgtt tatgactgtt
    29341 tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga ccgctacact
    29401 cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt catcgtctcc
    29461 gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata atatgatttc
    29521 cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc ttcattagat
    29581 agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat cagtaattgt
    29641 aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat atttgtgatc
    29701 tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt atcttcaact
    29761 aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat tttcgtccaa
    29821 ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc caagtacgct
    29881 tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa aagttttatc
    29941 atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt tgttgcttga
    30001 aaacctaact tccgataaag tctttgtgca tcttgattac tcatacgcac ttctaatgac
    30061 atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag cagcgaaccg
    30121 attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca atctaaaccg
    30181 aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc tagatataga
    30241 tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg caattcgcca
    30301 ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt caattctctt
    30361 agtacgtaac gacgatcgat ttgttcgatt gttttttctg tataaatcat tttatcttga
    30421 aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg
    30481 cgtctccaac catttttctt ctgcttctac ccgttttaaa tagcgaggaa gaaaagcttg
    30541 tttctctgca actggttcag ctaaagcacc taattgagcc acagttacac cacttggaat
    30601 atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta tttcatagaa
    30661 gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt gtagttgtgt
    30721 gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca atttctaatc tattttcttt
    30781 ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg gcacaataat
    30841 tttatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc ctactagctc
    30901 tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa gccctgtata
    30961 agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta tttgaagatc
    31021 tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt tgatatttgt
    31081 ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag tttggtttgc
    31141 agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat tctcgccttc
    31201 cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac tagtacaatg
    31261 atacatgatt atctgctggc gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg
    31321 gtatcacttc atttccccac agcttttcca tcttttattt cgttgattct cgaataatca
    31381 actctgtttc cacaataatc tttcttgaaa ccggtgcttt ggaattactt aattgaagaa
    31441 tcgtctccac tgctagctct cccatccatt cggtataaac ttttacagta gaaagagagg
    31501 gcgttacata tttcgctaca ctgacatcat tgaaacccat cacagaaata tcttctggta
    31561 ccactaaacc tttttcttga atcgctttca gtgcgccaat cgctaatgca tcattagctg
    31621 caaaaagcgc atcaggaaca tgcggattcg tttttaaata ctgcttcact gcttgatatc
    31681 ctgactcgac agaaaaatct gcttcgataa ttggaccttc gtcgtagcct ttttctgcca
    31741 aaacttgttg aaatgccata attctgggat cttttagccg ctgatggttt ttcttcgtat
    31801 attctgttcc agcaagcata gcgatatgtt gcgctttatg ggagacaaaa tgttcaatga
    31861 ctttttgaac acttgactta aagtcgacaa ctagagagtc gattccttga gaagtgccgt
    31921 cagaatctac caaaagcaag gcattttggg atcctttcat ttctaataac tgagcttcgt
    31981 cgaattttcc tagtgcgatc gttccatctg tcggaagatc actaatggaa tcccacgttt
    32041 ctttcaataa agtaacgttt gcttcttctg cctttttttc gattcctaga cgaatagata
    32101 aataataaag atcttcaagc tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt
    32161 gcttagtatt tgctgcattt ttacgatgtt tcgtataatt taagcgttct gctgcttcaa
    32221 aaattttttg ctttgtggct aagccaacgg aaagatcagg atcatagttg agaactcttg
    32281 aaacagtagc aggagaaact cctgccaact gcgcaatgtc tttaattgtt gccatttgtc
    32341 tctcctcctt ttttacgttt ataaaattgc agtaaatcgt tcaaacgctg cctgtccttt
    32401 ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt gcaaaaacct gacctacaga
    32461 tttttgtacg acatcggtga ccgtatcttt cgtcaaatca ggatgttctt tttttaattg
    32521 ttctgcccac ggacgatgat attcagccat ctggttttct tctcctaata agaaatgctc
    32581 tacttctact agttctggtt tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat
    32641 caacccaatg ttctcctttt tgatatgttg gacatcttta tgtggatgaa aaatcccatc
    32701 aggatgttct tctgaaacat tgttgtctcg aaggacaagg tccaactcaa agtaattgtc
    32761 ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta ccatctgctg aaaaagctct
    32821 gatagatact gtttcatccg aatatgcttt ccacttttcc aatactgagt tagctgcttc
    32881 aatcaacaat tttttctctg ttgattgcaa acggatcaca gacatcggcc atttcacgac
    32941 tcctgcgatg acttgcggat aatctgctag tttgataagt ttttctatcg gtgcttttgc
    33001 cataggaaat tcatgacgtc ctgcctgata atgatcatga gaaagaatcg atcctccaac
    33061 aatcggcaag tcagcattcg aaccaacaaa gtaatgaggc aagacttcag taatccgaag
    33121 cagtctagca aatgtttcct ttgtgatttt catcgggcgg tgttgttctg ataaaatgat
    33181 cgaatgttca ttgtaataag cataaggaga atattggaat ccccagcttt caccgtctag
    33241 attcattcga ataatccggt gattggtgcg ggctggatag ttcaagcgac ctctatatcc
    33301 ttcgttttcc atgcaaagca tgcaggtagg atagtctact tttaccgcct cgcgttctgc
    33361 tgcgatttgt tttggatctt tttctggctt agataaattg attgtgattt ccagttcacc
    33421 atactccgac tctgccggaa atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc
    33481 atttttctta cttaaattaa agaaatactc agttgcttcc tctgggcttt tcgaatagtg
    33541 ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc
    33601 ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca ccattttctc ttgccttagc
    33661 gacaagctga tccaataagt ctacagattc ttccttaact ggtcttactt caatgctttc
    33721 tagagcatct tctcctacca tccctaaaag acgattctgt aaatataagc ggtccatttc
    33781 catccatccg ccagcttcga tcgcaagtgt ggcaaaatca caaatcgttt gactgattga
    33841 catatctcat tcccctattc ttcataacca tttggatggc tgacatgcca atcccatgct
    33901 gttttgataa ttgctttgat atctgtgtat tcaggtttcc aacccagaac ctctctcgct
    33961 ttttcacttg aagcgactaa tcggctagga tctccagctc ttcttggtgc tatttctgct
    34021 ggaatttctt ttcctgtcac ttctctagct gcttctagca tttccttgac tgagtatcct
    34081 ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt ctttcaagta ttccaaagct
    34141 aaaatatgtg cagcaatcaa atcttctact tgaacataat ctctgataca tgttccatct
    34201 ggtgtatcat aatcgtctcc ataaacagcc aatgcttttc tttgaccaag agctacttgt
    34261 aaaatgattg ggacaagatg cgtttctggt gtatgatctt ctccgataga tgcatctgct
    34321 tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc gcatgccata tgcttggtca
    34381 caccacttca tcattttttc catcatcaat ttactttcac catacggatt tttaggatta
    34441 gctggtgtat cttctgtgat tggagactct tttggttctc cgtaagttgc tgcagtagaa
    34501 gagaacacga ttttattcac attgaactcg tgcatgactt caagtaagat ttgcataccg
    34561 tatacgttgt tgttgaaata catcaatggt ttttcgacag attctcctac taaagagctt
    34621 gcagcaaaat ggattactcc ttcaatcggt tccttttcaa atacggaacg tagaaattct
    34681 ttatcccgga tgtctccttc ataaaaatga gcatctggat gtactgcttg tttgtgacct
    34741 gtgagcaaat tatctaccac agctacttga tacccctttt gaactagctg gtctacagcg
    34801 tgcgaaccga tataaccggc tccgcctagt actaaaattg tcataaaaaa cactcctttg
    34861 attagtaaaa ttgtgtttgt tgattatata taatttaaaa aaacgaagtt tacttttatg
    34921 gtgaaaacaa acaaagtaaa atttcgtaaa ttcatatggt aaatttataa taaaacattt
    34981 agtaaacatt ttcaaggatt ttttgcaaag gtttacaaac aaacgtaaaa ctagcctttt
    35041 tttctctttt gttccataat agatataaca aataataaat gaggtggatt caatgaaaaa
    35101 atttgtatct ggaatattag taggaagtct ggcaacagcc gctgccgttg ctggtttggt
    35161 cgcttctgtg aaaaaaacag tgatcgatcc aattgacgaa aaagaagcaa tgatcgaaga
    35221 aaaccgtaaa aaagcaatgc gcaaacgcgt ttctcgttaa taattattta aaaacacgca
    35281 tgaagacttg tgaaagaaca gataagtcat catgcgtatt tttatttgtc agtcaatata
    35341 agttgtgcta ttttatctgc ctgtctcaga ttccatggat ctgtataagg tctagctact
    35401 gctgcagtta gccaatccac accttcaaca ggtattccat agcaataaaa gtcagactgg
    35461 atttcccctg aagcatcaat cacgcgattc gtacctcgct caacatctat agcttctgaa
    35521 tgataacttc ctgtattatc tgaaaatgaa aaagggcgaa caagtttgtt ttccttcaaa
    35581 ttttttatga caggattact tgaacgagaa aagcttgttt gtggcaatcg agcttcaatc
    35641 aaatagcgac tgctgatttc tttatcttgt agagaaataa catagcggtt gtgcttttgc
    35701 tgaatatcaa atggcggttc gaacaaatga aaaatacccg catctatcag tgctgctaat
    35761 tctttcgtcc gaatgactgg cggaccgatc gttaaaaagc tatttagatg agtaaaccat
    35821 ccccatagta tttctttgta ttcctttgca gaaaaaacgt gccattcaat agcctgccgg
    35881 attggatcac gccagtcttt taatgcatca aatgttgaag caaatgctcc tgtgcaattg
    35941 cctttttccg cttcttttat ctgcccttca ataaatgctc ggctagcttc tgcaaaggac
    36001 tcatcctgat aggtaagagg aatttctaaa tacgaccaat cccatctata agggattagt
    36061 gctggatgct ttccgagaca ctcctctgaa gagagggaga gaaaatcatg ctcaaaattt
    36121 ttctttggaa tcggcaaatg atattcttcg atgattttct tgtaataaaa caactcaacg
    36181 tcttttttca gatagtcaaa aaatgtttcg ccagtcaaaa atcccgactg atgccattgc
    36241 tccaggtttt cttttgttag taaccgtggc catgccattg atccgccttg cttttggttt
    36301 cttcctcgag ggtaataagg gagccccctt cttgatccac tgtatacaat cggttccttg
    36361 cctgatggat gatagacaag ttttcctttt tcgttctcga agcgcccttc tcttgatact
    36421 gtaaacaatc cgacataatc aaaaaaactt aagcctagac ctcttaaaaa gacattttct
    36481 ttaggcggta tcttcgaaag atcgacatct gcaggattag aaggaagctg atacagtaaa
    36541 tcatttttct ttgcgtagtc tacccacact ctttcttctt ctgtctcatc attttcccag
    36601 tgaccgcttg ctaaaacaag ctgatcgata gaatagcttt gctgatctgt ctgtaaaaga
    36661 taaccatctt ttcttttgct gacttttttt actaaagaat aaacaaactg aatagaagct
    36721 ggaaattctt cttgcaactt tgagaaaaac cagcgttgat agagtccgta taaacatcga
    36781 gtagattgct gatctttttc cagacgattg atttcttcta taattagttg cttatttttg
    36841 gttaccgtct gttcgatata atctgtagca ggtcctttac tccattgaaa caagttgggt
    36901 ccctgtgtta ccaatcctcc actggacaat gtttcatctg taaaaagcgt cacttgttga
    36961 gaaacagaat tcattaataa ctccggtggc tgattcaacc gccaaactcg acctccagga
    37021 ccattgggat cgaaaagaaa aagttcaaga gaatgtctgt cctgattatt atggagaatc
    37081 ctttctgcta tacttaatcc tctaggtccg gcaccaatga tccctatttt catttgcatg
    37141 cctccatttc gataaatttt ttcaataaaa cagcatggtt ctgatgttca ttttttgcac
    37201 cataaacaaa agtaatgact ggatgttctt tcataagttg actgagttta aaaatagctt
    37261 gcttcttttc ttcttcgaaa atcaattctt gctggtattt ctttgcaaat gaagcaaatc
    37321 gttctggtac atggtcaaaa gttttgcgtg cttcattact aggtgcaatt tctttcaacc
    37381 acagatctaa ctgcgctttt tcttttgaaa ttcctcgtgg ccagaaccta tccacgagta
    37441 ttctatagcc atcctctttt tcagcatcaa ggtaaacacg ttttaatttg atcatagttg
    37501 tttccttctt tctgcctttt cgcttagctt atatatgtag ataatagcac aaaataaaaa
    37561 aaatgaacgg cacaactatc cattgcgccg ttcatgtgat cttggtcaaa atctaatgat
    37621 tgattcggtt tttaaagctg tcatacaatc atctgtttta tactttttta cttttgattt
    37681 ttccagacca tccgtcatat ccacccttaa gtaaataaat ctcttgataa cctgctttac
    37741 gtaatttatt tgctgtacgg atgctcaagc ttttcttttg atcgtaaata tagacaggct
    37801 ggtcttttcg aatagatcct aatgaatctt tcaaaacagt gtaagggatg tttcttgcac
    37861 ctaaaatatg cccagcatca aacacatctt tttcgcgaac atcgattact tgtgcttttc
    37921 tcattccttc tcgaaattct tcttctgtca aagtcgttgc tgaacgcttg gccattactc
    37981 gtagatagac ttcccaaaag atgattgcaa gcacaatcaa tagcaaaatg atattaatta
    38041 cccataaaac catgtaatgc ctccgttttt ttaagcaaat ttcaaggcta atgaggcagc
    38101 accaatgaca ccagcttcat taccaagctc tgctaatttg atttttgttg attctcttac
    38161 ttgcgggaaa gtaaattctt taaaatattt ctctacacgg ctacgtaaaa attctcctgc
    38221 tgccgatact cctccaccta acacaatact tgacggattc aatgtattcc ccaagttggc
    38281 gcaagctaaa cctaagtaga agcatacgcg atcaacaacc atcaatgcaa acggatcgtc
    38341 ttcttgagcc atttcaaaga tatctttact tgttacttct tcaccgttgt ctaacattgc
    38401 tttcagtttt gaatctcctg cgtattcttc tgccaagtaa cgacctagac gtaccacacc
    38461 agtagcgcta gacacagttt ctaaacatcc gcgtttaccg caagtacatt cgaacccttc
    38521 aggatccaca gtgatatggc cgatctctcc gcctgctccg ctcacgccgt gaatcaaatt
    38581 tccgccagca acgataccac cgccaacacc tgttcctaga gtaacgaata caacgtctgg
    38641 atcgttttct ccagcacctt tccatcgttc acctaaagca gcaacattcg catcattatc
    38701 gatagcgaat ttgattcctg ttcctgcctc gatcagtttt ttcacaaatt gaacttctgt
    38761 ccagtttaag ttatacgcac caataaccgt tccagcttcg ctgtccacgc ttcctggggt
    38821 acccatgccg attccgataa agtcttcggc tttcatatta tataaattca aacgatggtt
    38881 gattgattca atgatttcag gaacgatatg cttaccatca tccaaaatat tggtatcgat
    38941 gctccatttt tgttgaatct ctccttctgg tgttaaaatt gcaaattttg ctgttgttcc
    39001 acctaaatca atcccaataa tcttcttgtc cattacaaat ttcctttctt tcggctttct
    39061 tcaattcggt gctctctttt taatatgcgc cacgctgtca tgtatgtatc atgatcaatc
    39121 aaacggctat catacagatt acgcaattcg atagtcatta attcaatgtc atagatgcgt
    39181 gcacccacat agataaagat gccaaatttt ttgaacagct gctgtacatc atataagctc
    39241 tccatcttta agcacttcct tcatattata caagtaaacc atattttttt aaacccagta
    39301 aaatacatat tacaaaaaga aatacaaaaa ctagtgtaga aagaatcctt tggtgtatcg
    39361 aatagcttgt ctgttgtttc ggtagaccca aaacattccc taacactaat ccgccaatta
    39421 gcccaccgac atgtccccaa acatctacag aggtatccag aagaccaaaa acaaatgtca
    39481 acacaataaa taaaagaaaa tgtctgacta gctgctggat cgctgggtta tgtttaaaat
    39541 gaaaccctaa gataaacaac gcaccgaaca aaccgaatat cgaagtactc gcaccaccag
    39601 acagaacatt cgcttcgtta aaagcaaagc ttgctgtatt tcccattatc ccgctgaaca
    39661 tataaatcaa gaaaaaacgc caatggccat agattgcctc gatttgctgc cccataaaat
    39721 acagtatcag tgagttcatt acgaaatgca tcagtccaaa atggatgaag attggtgtca
    39781 ctaaacgcca ccattcattg aagtgaacaa gataaggacc atacattccg gcataataag
    39841 ggatctcaag tcgcggaaat aattccatca tgaggaagac cgctgtctgt atccctaaaa
    39901 aagcataggt ccataacggt cgtcttaacc atgcacgcaa ttttatttgc tgttgataat
    39961 tcatgaaaac ctcccatttc attttagtca ataaacaatt gttggatctt ctgatcaaag
    40021 ctctccggtt gccattgttc attcaattgt tcacgaaaaa caaggctaca tgttttttta
    40081 tgaaaattat ttaaataccg gtcgtaataa ccaccgccaa aaccgattcg ataaccggct
    40141 ctattgtaga taatacctgg tacaatgagg agatcgatca tctccggcaa atagagcttc
    40201 tctgattgtg gctcaagaac accaaaactc gaacgtacaa gaacggactg ttcatccatt
    40261 tcataaaacg ccatctgacg tttcggcaaa gttttaggta caactatttt cttttgttct
    40321 ttttctgctc gttggataac cggctgtgta tccagttcaa attcattcga gaaagtcgtc
    40381 cctatcactt ttgctgactt ccagtactcg ctggcgaata gctgtttata gattcgctgt
    40441 tctttttcct ttttcttgcc acttttggcc agatttttca gcttttcgat tcctttccgg
    40501 cgtaatctta ctttcaattg gtccaaaaac tcacccttcc ttctctcata aaatggtata
    40561 ggaaaaaaag aaaaatagca atcgaacttt caaataaatg cataaataga ataaaaagaa
    40621 gacctagaaa tgattgacct tcctttttta atgaatttca actgttcagc gcgtgttttt
    40681 gtctgataaa aagtgcatac agaaaatatg gcgcaccaaa aagggcaatc aaaattcctg
    40741 taggtacttc cccacttggt agaatcactc tagcaaccat atctgcacta agcacaaaaa
    40801 tggaaccgaa caaaccgctc agaacaaagg tccagttatt ttctttacgt acgactagct
    40861 tcgcagcatg aggagctagt agtcctacaa aagataaact acctgtcacc gctacactac
    40921 cgcttgccaa agcaacagct aacaacaaaa ggattttttg acttttagcc acgttaaggc
    40981 ctaatccttg cgcgcgttct tgaccaaatg atagcaatcc aatcctctgt gcataaagtc
    41041 ctcctagcgg aattaagata accgcccatg gaagcaatgc ccaaacataa ggccagctcg
    41101 caccccagat tgatcccgct aaccatgcat tcacaaatcc ataattttcc ttagataatt
    41161 ggatcgtgca tagcaatgtt aatgcagaaa ggccggcatt aacggcaata ccggacaata
    41221 agattcgatt catacccaaa aagccatttc gatcataggc cgcaagatag acacagcaag
    41281 cagctaaaac accacctaga aaggctacaa ctagcaatag aaacggcagc gaatagtgcg
    41341 aaaagaaacc taaataaagc atcactaaaa atcctgctcc ggcattaatg cccaatatac
    41401 tagaatctgc caattcattt cttgttactc cttgcaagat ataaccagaa acgccaagac
    41461 aagctcctgc taataaactg atcagtaatc gaggaactcg aaattctcta acaatcagcg
    41521 ccaaagaaga tgattctttc cctatgaacg tggcaaaaag ctgatccagg taaataaaag
    41581 gcgtaccaat cattaaacta cctatagaca ggatgacaag tacaaaaagc aagaaataaa
    41641 acgtccaata tatttttttc accgttgttc cctccttact tgtatcagta aaaacggaac
    41701 gccaatcaat gcaatgacta atccaaaagg tgtttcaaaa gggggattga tcatcctcgc
    41761 tgtaaaatcc gccaacataa ccaatccacc gccaactaaa aaagagacag gtagaacata
    41821 acgatagtcc gttccaacca ttccacgaac aactggcgga accaacagtc caataaagga
    41881 gactggaccg actaacgaaa ctgctatggc tgccaacact gcgactagac agaaaacaat
    41941 caaccgcgtt cgtttaggat gacttccaag agctaatgca tgtgtttcac tcaaatgaat
    42001 caaagatatt gcttttccta atgaaaaaga aaccaaaaca gcaagtatat ataaaggaaa
    42061 caatactgct aattgtttcc atgtgatatt ggcagctccg cctacaaacc aaaaagcaag
    42121 atcctgctgc agattaaaga attgggtcag tgcttggctg atagcagtaa aaaaagaact
    42181 aatagctacg ccagctaaaa tcagctgtaa aggattcatg cccattttcg aaaactgcaa
    42241 aaaaccaaat acgagcaaag ttacagcaaa agaacctgcc aatgacatta gaaatacaga
    42301 aaaatcagaa gctttaggcc aaaaagcaaa actaagggcc attgccaacg aagcacccgc
    42361 gttgattcct agtaagcctg aatctgctaa aggatttctt gtcactcctt gcatcaatgc
    42421 accgctcaat gcaaaacaac tccctacaag aaatgctccc gccacacgag gcaaacgaat
    42481 atttcgtatg atttggtgac tttgttttga tctatcaaaa tcaaacaacg cctgccaaaa
    42541 atctgctttt cccacagtcg ctgcgccatt tgaaagagca acaaaaaaaa taatcaaaag
    42601 aacaaaacag accaaaagca ggagtctatt tttcttcata gtcctcataa ttgatcctcc
    42661 tgctcttttg gcaacaaatc ataggttaga aaaactggtt tatttgttct tggaagcgtg
    42721 acaatcgttg catcaatctc aaatattttt ttcatacact cttttgtgat cacttcttca
    42781 ggtgtacctt ctgtgatcaa cttaccgttt ttcattgcta ataaataatc tgaaaaacgt
    42841 gaagcatgat taatgtcatg aatcgacatc aagatcgttg tttgtttcat ttgattgatt
    42901 tcagtcaata actgtaggat ctctaattgg tgtgccggat ctaaaaatgt tgtcggttca
    42961 tccaagatca agatatctgt ttcctgagca agcgccatag cgatccagac acgctgggtt
    43021 tgtccaccag acagcgttgc gatatcccgc atacgaaatc cagctagacc tgtagcatcc
    43081 aacgcccact cgattgcttc atgatcttgc gtgtccatgc cactgaacag tttttgataa
    43141 gggtatcgtc cgtaagatac tacttcttct actgtcatac ctgtagggtg ttcactagtt
    43201 tgtgctaata atgcaatttt ttttgcaagt tcttttgaac tgtatgtaga gatatcagag
    43261 ttattgacaa tgatctttcc tgatttcaca ggtagtatac gggtaaaact tttcaataaa
    43321 gtagattttc cacttccatt tggcccaatc aaactagtga ttttcccttc aggaatcgta
    43381 acagacatgt tttcaatgat ttttttgttt tcgtagccgg ctgcaatgtc agctgctctt
    43441 aatctcgtca tttcacccat cctttcgctg aaaatcattc tcaattatga cgctgtttca
    43501 acatttcgtc aagcgctact ttcatatcta tagccaaatg aagcggttga cagcgttcga
    43561 aatatcagga ataataacca atgagaaaga ttctcaatta tgtcaggagg aaagaaatga
    43621 aattaaaaac aaaagtattc acattatcta gcatattgct gctggctgga gtactgagcg
    43681 cttgcttccc ctcttcagat aaagaaacag ctacaactgc caattcgacg acttcatcca
    43741 atagtactca tacagtcaca gatacacttg gacataaagt agagattcca aacgcgccca
    43801 aacggatcat cggcagttac ttagaagact atctgattgc actaggggaa aaacctatcg
    43861 cccagtggac tgttggcagt ggttccattc aagattatct gcaagatgat ttaaaggatg
    43921 tcccaacgat ttcttatgac cttccttatg agaaagtgtt aagctttgaa cctgatttgc
    43981 tgttgatttc ttcttccgct actgtcgaag gcgggaaata cgaacaatat agcaaaatcg
    44041 cgccaactta tgttgtaaaa aacggaacag atgtcacatg ggaagaacaa ctaaaagatg
    44101 tcggcaaagc tttggcaaaa gaaaaagagg cagaaaaaat catttccgat tatcaaaaat
    44161 ccatcaagaa aactcgagaa gaactagcag ataaaatcga gaacaagaca gctgcagtgt
    44221 tgtgggtgac caacaactca gcctttatgg tcagcaaaaa tcggtcaagt ggaagaatcg
    44281 tatacgacga tctggaattt ggtgttcctg atctagtgga agaggtctcc aaaaaagcca
    44341 cttctgactg gtctgctgtt tcctctgaaa agctagctga attagatgct gactacatca
    44401 tccttgtgaa cagtgacaaa gatgctgcca tgttcaatga accaagctgg aagaatctta
    44461 aagcagtgac agacaatcat gtactgcaat tcggacctga atctagctgg ctatataacg
    44521 gacctattgc cagtcaggaa atggtcaatg atatcaaaga aaaactaaat tgattttaac
    44581 taaaaaaagc atcttcacga ttagtcgtga gatgcttttg ttcttattaa caaactagac
    44641 gcatagattt tattcttctt ttgaagcaac cctatttttt actctttccc atttttgaaa
    44701 acgatgggca tatagatttt cttgatccgt tatcccctca tactcctcta taagggcgaa
    44761 gtctgaaaaa tcaatattcc caatatacgt atcgccttca aacgtgctat cgatcaatgt
    44821 tcgccagatc cgaccagtgt aaggaagaag ttcttgaaaa atccgactac cacccgaaac
    44881 atagatatct tctgagatcg tttgactata cgacaaaagt tcatcaatcg aatgcattac
    44941 ttccgcattt tctttcgtac ggaaatttgg ttgcgttgtc aacacgatga tatgacgata
    45001 aggaagtgaa agatccccca ttccttcata ggtctttctt cccatcacta aaattcgatc
    45061 cattgtatgt tcgcgaaaga aacgcatatc atttggcaat cgccatggta acaaaccatt
    45121 tttgccgatc agaccatttt tatcttgtgc ccacatggaa ataaacatat tatccgctcc
    45181 ttatgatttt agatgttttg agataaaaaa agtctcaatt ctttgaccag caaggaattg
    45241 agaactttaa aaacattatt ttgtttcacg gtgcaaagta actttgcgtt cgcgtgggca
    45301 atatttttgt ttttccaaac gatcaggatt gttacgtttg tttttgcttg ttagatagtt
    45361 acgttcttta caagaagtac actctaaagt gatgtttacg cgcatgattt tccctcccat
    45421 ttttacaaga atcctctaag atgactctca gacttgatta tcttaccatg attttttcca
    45481 gattgctagt gtttttcacg attttgttaa gtaaaactac ttgacgataa atacaacggt
    45541 tttacttctc agacaaaatc tttttctttt tgtataaata cccttacact tgtttataga
    45601 gactatgttt ttttaaataa agggggatac taatatgcca tggaacatga aggactttcc
    45661 tgcatcaatg aaaaacttag ataaactgac aagaaaaaag gcgatcgaca tcgcaaatgc
    45721 tctattagac gagggttacc ctgatagccg tgcgatccca attgccattg atcaagcaaa
    45781 agagtggtat gaaaatgcta gtgaatcaga aagacgaaca ttcgaaaaag aaaaaaatcc
    45841 aagtaagtcg gataaacatg atacgaatcc tcgtgccgga aaattgcttg attcagatgt
    45901 gatcgtagaa tatgccgaag aacaatggat cgtgaaatct aaaggagcaa aaaaggctag
    45961 caaccatttt gatacaaaga aagaagcgat cgaaaaagga aagcaagtag cacaaaacaa
    46021 agaatcaact ttggtcattt ataaaaaaga tgggacaaaa gaaaaagaaa tcgattattg
    46081 aacatatgta aaggggttgt gacgaaaatc gtgtcacaac cccttatgtt cgaaacaatg
    46141 gtgttcctct gccaaatccg aaccacaagt cataatatct ataaaacggg aagagctgtt
    46201 ttttttaatt gtttcttatc actcggtgtt aaacggtagg agcaaaccta ttttttattg
    46261 atatttgatt ttcatcaaac agtcacatag tcgattgctt ctgcttgcgg aatcgtttca
    46321 tctacaaagt agactttata ccatgtcaag aaactgaaga ttgtccagat ttttctttgc
    46381 tcttctttac gtccttcgtg atggtcatct agcaacttca tgattttttc ttgatcaaaa
    46441 aattcttttg caaaatctgc ttcgaataat gctttgactt gttcgtagcc gtgttcttct
    46501 ttcaaccaag ctttgattgg cacagggaat cctaatttca cacgattcga ccattcttcc
    46561 ggaagatgtt tgttcgcagc ttgtctgaaa atatcttttg tgttgttttg attcaataag
    46621 aacttacttg gtatttgttg agccaatttc atgacttcga tatccaataa aggtactcgg
    46681 acttccaaag aacttgccat agaaagttta tctgccttca acaagatatc ttttggcatc
    46741 cattgatgaa gatcgacata ttgcatctta ttgacttcat ctttgattcc ttctgttttc
    46801 tcgtaatgaa cagtcatgat ttcatccaca gatggtgctg tttgatattt tggtgtcaac
    46861 acttctgatg cttctgattc ttcgaacact cgagcatgac cgataaaata ttcttttgca
    46921 ggagcaagtg attcatataa atggatccgt ccatggaaat ttttcatttt tccgatttta
    46981 cgtcctaaat tatagcgtgt tccttttggt aattttttca atccttgagc aatcacacgg
    47041 atgaattttg aattcgtatg gaatccatac gtttggtatc ccgcaaacag ttcatccgcc
    47101 ccttctccag attggactac ccgcacgctt tgtgaagcaa gatttgctaa aaagtacaaa
    47161 ggtacgcaag aaggatttga atcaggttca tctaagtgat actggatcaa agggaatgct
    47221 ttgaacgaca tgtctccgtc gatcactgct gctgtattat ctaaatccaa tagttcggtt
    47281 agttttctgg cttcaatcgc ttcattatag gttttatcat caaacccgat tgagaaagaa
    47341 tgatctggtt tcaatacaga ggttacataa ctggaatcaa ccccgctgga taagaaagaa
    47401 ccaacttcta catcactgac cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt
    47461 ttttcgatcc attcttgtcg ggaatgcgtt tcttttgttt caaaatctgc atcccaatat
    47521 tgctgaatgt ctagttttcc atctttatac gtataataat ggccttcagg caaacgatag
    47581 acatctttga agaatgtttc gccgtttaat ggagaatatt ggaaagtcat ataaggtttc
    47641 aacgcttctt tgttcaattc tttgttaaaa tcaggatgag gcagaaaact tttgatttct
    47701 gaaccaaaca taaatgttcc gttcatttct gcataataat atggtttgat cccaaagtgg
    47761 tctcttgcac cgaataactc attcttttca ttatcccaaa tagcaaaagc aaacattccg
    47821 cggatctttt gcaataattc tgttccccat tcttcgtaac catgcaaaag gacttctgta
    47881 tctgcatgtg tttgaaaaac gtggccagct gcaatcaatt cttctctcaa aggctggtaa
    47941 ttatagattt cgccattaaa aataatgatc tttgtccgat cttcattgta aataggctga
    48001 gtaccgccct ctaagtcaat aatacttaat cttctgaatc ctaaagcaac atgtttatcg
    48061 atatattctc ctgaactatt aggtccacgg tgaacgattc gatccatcat tgtattaata
    48121 attgtttttt tgttgtcctt gtcgttaaca aaacctacaa taccgcacat ttaactttca
    48181 catcctaata taaaattact gtttatttca ttcatatgca tcttatcata gaataaatag
    48241 cggacgaata ttttctctct tacagtttcg tcacgtgaaa acaaaagatg aaagtttctg
    48301 tttttacaga aaataaagtc ccaatatcta atttaccatg ttttcttttt tgaggctatc
    48361 ttattctctt cgtgttaaaa aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa
    48421 aagacacgac ctaaagatcg tgtctaatta tgactgtatc agaaaatatg ataccaaacg
    48481 gatctattca ttaattaatt gacatagttt atctgttgct cttttatgac tggataactg
    48541 accaattcat gacgatccaa gtcttcttta ttcatattta cagcagtaat ttgactgtct
    48601 tcatacgtac gataataata aatcccttta tcaacgttac agcaagaaga ataaatcgta
    48661 tattcgtatt ttccttctcc agtatcgcat actccttttt gttgctcgac tgagcctagt
    48721 atatgaaaaa attgactgat actttcagat tctgaatcac ctgatgcagc attcatttta
    48781 gtaaatgtgg cttttacaaa tcgagaaaca gaagagagaa ctccaggtaa gcccaatcct
    48841 cccatcccac gactataaac atccaagctg atctggtttg aaaaatgatt ttctggtgtt
    48901 ccatttgata gcgagcgata attgttcaga ttaaataatt gataatcaaa tggtggatta
    48961 tttgtcaaca caccgacagg attatcatat aaatgaagtc catctttcat actttcaatt
    49021 actatcgatt tttctttatc agctaataac cagtgtaaag gtgacaaggg tagtttttca
    49081 ctaaaatcga cattgactag attgatagta gccaataatt ctttagcttc gtctatggtt
    49141 gagcattgcc ctagtatcca tggaataaac tcaaatggtg caacgttatc ttttccttct
    49201 tgtaattctt tataatcagc attgccagga aaattcagtc ctgccatact caatcctttt
    49261 tcatttgtag cctcataata gagtggatag ttggctaccc cagctgctat cccaatcatt
    49321 gcataatgtt tatctaaatt ttttaccttt ctaaaatcga aacgataatt tcttggagta
    49381 atagtgacta cttcattata agacatctca taatcaaaat tccttccaaa ataatggtct
    49441 ttcgtcgcat aagtgattgc ggtacacatc gttttccctc ctaaaacaaa tcgtttttac
    49501 acttttatct tacatctagt attttttttt atcaaaaaaa aggccaaaga agattgattt
    49561 actagcattt ataaaaaata gtactcaggg ggaatcaaaa aatattgtag gctaaaagta
    49621 caacaaaaat aaggggctgt gacaaaagcc ttgtcacagc cccttattcc gaacaaacgg
    49681 tgttcaaaag cttgcatctg cggtaataag tccaaccaaa cgaaaaaata ggaaaagcta
    49741 tttttgtttg gccgttctta ttacttgatg caggacagct tctttacaac ctctaatcgt
    49801 tgatataagg aaggtacagg atttgaacct gcgcgccggt attagccggt tcgccggatt
    49861 tcgagtccgg tgcattacca ctctgccaac cttccgtaag tactattttt aaactttttc
    49921 cacgaaatgt caagcctatt tctaatagat aaataacaga aatcgctttt tttagcaaaa
    49981 aagtccattg acagtttgca gccaacggac ttaccgatca aattcatact gatagaacac
    50041 tatctgatta tttcaggatt atctaatgct aaaatcgagt caattgtccg tttgatcagt
    50101 gcaatgattt gttcttctgt tggatgcatg atatttgatg acatcaatga cgctaagcca
    50161 gtcactgcta cccacgtacc agtatgcaga gcatcgacat attcatccga taattgattg
    50221 tatctttcat cttctttaac tatttcacag aaataattgt atgaaaaatc atgcattttc
    50281 tttccgtccc catgttcatc aatgtacaat gccatgtaaa gactatgatg cttttttgca
    50341 aagtagatat agttcaaacc gagatcaatg attttatcac cagtacgttc aatagggaaa
    50401 acatcttctt ttagttgttc aaagatagaa tccagcaacg tattttttaa atcttccatg
    50461 tttttgaatt ccagataaat cggctgtgtc gaaatcccca tctttttagc aatatttctc
    50521 gcagtaaatc ctttaaaccc ttcctttgca acaacatcat aagcagcttt tagaatttga
    50581 tccttggtat agacttttct tctcattaaa actcccccac ttctttactg cagtatatgt
    50641 tgttttattg taccctattt ttagcatttt ttgcaaacat tttactcaaa acgttatatt
    50701 ttaaattttt ttctcagtct tctttaactt tttttgcaaa attaaaaaaa tttggttaaa
    50761 ttcaatgggt tcccttacat ttttagtttt gacctaaatg atagttgacc aaatctttca
    50821 tttcaagtta tagttaaaga aaatgaaaaa gaaggtgtgt aatggaaaac gaaactgacg
    50881 ttatttatat tcatcctcaa aaacgaatcg tttcacagaa aagaaagtat ttttatcttg
    50941 gttttacagg tgcttttttt ctttttattg gcttgctttc tggtactcct actgataatt
    51001 ggagtggatt gctaactatt cttacctcgc caagcaatct tttgacagat tattttgatt
    51061 taggcggttt cggaagcgca ttcataaatg tagggatttt gaccttactt tctgtgcttt
    51121 tagcttatcg gcacaaggtg attttaaacg gtcccttatt tgcttcgatt ctaacagtta
    51181 ccggtttctc tttttttggc aaaaaccttt ataattctat ctcaatcatt ttaggtgtgt
    51241 atctatacgc tgtatttgtc aataaacctt tttctcagta catcatgatc ggactgtttg
    51301 gtagcgcatt atcaccggtc gttagttata tcacttttgg tatgcgtttt cctttactga
    51361 taggtatttt attggggaat ttagctggca tagcaattgg tctacttctt ccgcccttag
    51421 ctgctcaaac tttagttttc catcgtggat tcacacttta taatattgga ttcacctctg
    51481 ggttgattgc gatgactttt actgctgttt tgaggttatt cagctattca atcgtcgaaa
    51541 atacattggt ttccaatgaa tatcactttc ctctcatttg gattattttt ggcttttttt
    51601 cgctgacagt cggaattggt ttctattata attcctttcg gctttcgggg attcgagaga
    51661 ttttcgattc atctggaaaa ttgacaactg atttcattgc taattctggt attggtgcta
    51721 ctttgatcaa catgggctta gttggtctga tgctgtcttc ttatgtttta ctggttggcg
    51781 gtcagttaaa tggacctgtc atcggtgcta ttttatctgc ggttggtttc agtgcttttg
    51841 gttgtcattt aaaaaacagt tttccgatac tagtcggaat atttattgct tctttgtttg
    51901 gaacttttca tgagatcacc agtactggca tgcttgtggc agctgtgttt gggacaggtc
    51961 tcgcgcccat aagcggtttt tacggcagtt tttacggtgt aatagctggt gtgctacata
    52021 ttgctcttgt ccataacgtc agcactttac acggaggact aaatctatat aacagtggtt
    52081 tttcaactgg tttcgttgca ggtatccttg taccgatact agataacttt accgctgtca
    52141 gaaaggaaaa aaagacactt ggaaaaagaa ttatcaaaaa gaatcatcga tgagcttctt
    52201 acttatttat attcccatga aataactgaa atacgtattg gagtagaatt tgctgaggaa
    52261 gggcttttca tagagataca gggtacggca gaaactcagc cagaagattt agatgtactt
    52321 gctgatttgc tgaacaatcc tcgggatcct agtatggaaa tctactatca ggaactatta
    52381 ggtaatcccc gacatgatcg ggaagattat catctgttag gtagtttgat tgatgacgtg
    52441 gaaatcatgt acgcccagcc gattatcaat attaagatct ttcgacgtgc ttctcctcgt
    52501 tcctattatt aaccctattt tctatttata aaaggctgaa tcaaaaaagc agctcctgaa
    52561 agttcgatta aaatctactt tcaggagctg ttcaagcttg acattattga ttttccaatc
    52621 cttcatacac ttcatctgtt acaggttcta aaaactctga gtttccggaa gtgatcgcta
    52681 aatgttcaaa ccaactgtct tttgtagcac catgccagtg tttgattcct ttgtgcgtca
    52741 ccacaacgtc tccagctctt aatcgtcttg ctggctttcc ttcttcctgg taccatcctt
    52801 ctccgccagt cacaagtaag atttgatatc catctgtatg gacatgccag tgattgcgac
    52861 aacctggttc aaatgttaca ttccctacac caacagaaac ttcgggatca ttcactaaca
    52921 tattcagata gctttgccca gtaaatacct gagcgtaagc ttcgtttttt tcccctgctg
    52981 gaaaaatcac gccattgtgt atttcttcgt attttgtcat catttacgcc tctttcttgt
    53041 ttacgctgtt tccttctaga tagatttctt ttgctaagtt aaaagctgac caagcttttg
    53101 gccatcctac ataaaaagct aaatgggtaa tgatttctgc tatttcttca ccagtaatgc
    53161 cattttgttt gccaatcttc agatgagctg gcaattgttc aaaatttcct cctgaaatta
    53221 ataaagatat ggtgatgata ctacgctgat gagcagataa ttcttctctg gaccatactt
    53281 gtccaaataa aacatcatca ttcaattctg caaattttgg tgcaaaatct cctaattgat
    53341 ctcgtcctgc ggtttgtttt tctgccattt ctcttattct cccttcacga ctgttaggtg
    53401 attcaactat ggttctaagg caactttatt ttctattgtt tcttcgcatt tcattttaaa
    53461 tgtagtcttt cagcctgtcc aatgcttagt ttattcagtc gatatgcttt ttaagcatag
    53521 ttgctaacgc tttttttatt taaaaaaaaa caagttatta aataagtctt tagttggatg
    53581 tttttttgag atcaatcttt tatacttatg atgaacttac aaaattgttt gttttctgtc
    53641 atatcaaaaa atggcgcagt tagttgcttt tctagtaaag tatagttaac aaagaacctc
    53701 taaggagttg atgaataatg aatattgctg cctctcgaaa actgataaat ttcatatcca
    53761 ttatcggatt aggcctatca atcgcgctga cgatttattt tattaattta ggtgttttca
    53821 aagatttgaa tgcgttaaga ggattagtag gtgattccat cattttagga ccgattattt
    53881 ttgtcttcat ccaaatcctg caagtagtta ttccaatcat tcctggaggg atcagtactg
    53941 cagctggtgt cctgatattt ggcccttatg ctggatttat ctataactat attggaatct
    54001 gcattggttc gattatcatt tttctgctag ggcgtcgtta tggaaagcca ttcatacttt
    54061 ccatgattag cgataagaca tataacaaat atattggctg gctggataat caaaaccgct
    54121 ttgagaagct gtttgctcta gctatttttc ttccagttgc tcctgacgat gcgttatgtt
    54181 tgatggctgg attgacaaac atgtcagtga aacgatatac actaatcatt ttgattgcta
    54241 aaccactgtc gattttctta tatagtatgg ccttgattta tggaggccaa tatctaagtg
    54301 gtcttcttgg ataatattta ttttgcaaaa ttagtcattg attgacaatg ttaaatctgg
    54361 gaaaggggct gcaaaacaca ctgcaccctt tcctttttca ttagatttgc gctacaatgt
    54421 gaagagcagg tgataggatg aattatttaa aacgtagctt aggtaatgct tgggactttt
    54481 taaaaggaac acaagcttat ttccgtgatg tcttgctgat gcatggcttt attttattta
    54541 tttgtttacc cttattgagc agtacgaccc gttttatttt acaccgaggg gcaattgact
    54601 atttgtctta tgacaatatt ccttcaattt tttctcaaca tcctggagtt ctactctcgt
    54661 taatcgtagt tttgatgatg attttactgt tagtttattt cgaatttact tttcttttga
    54721 tgagtgtctt ttttattaaa aagaaagaac caatctcttt gaagcaactc ttacacttga
    54781 cgatacttca gctgaagaaa gtgagaccca ttactttttt gtttttcctt gcttattttt
    54841 tattgattct accgatcagc ggattaagtt ttaattctga tctactgtca aaaataaaaa
    54901 tccccgcttt cattatggat tttatttttg ctaaccgttg gatcatcgtc agtttctttt
    54961 tactcgttta tattttcctt ggctatattg ggattcgact gatttttgct ttaccggaaa
    55021 tgattctacg agatcgtcct tttcgagcag ccattcggga aagctggtca ttgaccaaat
    55081 cgcgtctttt ggccatcacg ggccaattta ttgtgattgg cggtacgatt ttactccttt
    55141 cttctttagg atatatcgtt gttattttgg cacaaagtat agttgaacaa tttttcccag
    55201 actatgcatt gatcagcgct gtgtttgcta tgacgctttt acaagggatt ttattattca
    55261 atatcgtgat gtctaccgta gggatttttt atatcattgt tgatttcatg gatgacgaag
    55321 gcttcttgcc tgagatccct aagtggttta tcccacaagc ccccaacttg cgtttctctg
    55381 cgctgaaaaa cactggtttg actctttttg cagttttttt cggtattggc gtctgtcttt
    55441 acaatatgga ttatctgact tcagctgttc agacaaaacc agtgactgtc tctcacagag
    55501 gtgtgagtac ccaaaatggg gcgcaaaata ctttagaagc acttgaaaaa acaagcagag
    55561 actatcatcc tgattatgta gagatggatg ttcaggaaac aaaagatggt catttcgtag
    55621 tgatgcatga tgcaaatctt cgtcacttaa caggggtaaa tggaacacca caggatctta
    55681 cattgaaaga attgacaaat cttacagtta ctgaaaacgg agtgcaagcc cctgtttgtt
    55741 cttttgattc ttatttggaa aaagcacata agctaaacca aaaattactt attgagatca
    55801 agacctctaa aaaagattcg gatgaccttg tcgaacgatt tgtccataaa taccgcaaag
    55861 ttatcttaga gaaccatcat attttgcaaa gtttgacgta ccaaaccgtt tcagatttaa
    55921 aagaagcaga accaaacttt tatgtgggct acatcttacc tttcaatatt gttgggcccc
    55981 ctgtgacgcc tgctgatttt ctgacaatgg aatatagtac gatcaaccgt aacttcatta
    56041 attctgccca ccaagacgga aaagaagtat atgtatggac aatcaacgat tcagatgcca
    56101 tgagtcgcat gatgttttat ggtgtggatg gtatcataac cgatgagatg ggactactaa
    56161 atgaaagtgt gaaacaaaca gatgaagaaa tcacttattc agataaactg ctaaattttg
    56221 ttttgggagt cggctaatca aaaatcgtta atgaaaaaag tctttataca acaaaaaacg
    56281 cataacctgc caccgcaggt atatgcgttt tttgtttgac gctcataatg tgtgcttaac
    56341 tttctcttga ttccttcatc gcacgaacaa tagctaaaat agcaggaatt gcagagatca
    56401 ctgcaagaat aattcccatt ttccctgaac tttttctttt tggcatatct tctcgctccc
    56461 ttctgtcttt atttatatta taagggagtt cttgcgaatt ctcaaaaata ctgtttaaga
    56521 aaccaattca gtaggtgcgc taatatcaca gaattttacg agcatgcgat acttgctttc
    56581 attgataagt tgatagtctc gttggtcata actttcaaca tacacgacag ccgtgttgat
    56641 atatgtacgt tcaatacagc cgattagttc atgagatact ccgatcggtt tgcatatata
    56701 agatcttcct acttctgctt ttaccatttc attccctctt ttctttattt ttattttgtt
    56761 tttctttatt ttataataac aaattacctt atatttgaaa acgtttttat tcattttttt
    56821 attttttatt ttgagtatct ttctgctttt tagataacta ttttttaaca cgatcttaga
    56881 cgttgtgaca aaaacgtttg tttatattcc tatgttgttc gtgcgttttt ttctttatat
    56941 tgctaagtta tttataaaga ggtgagcata tgatttatat tcaagcaata gtatttgtaa
    57001 ccttgatgat tgggctactt atcggattaa aagaaaatag ctaaaccatt taagaaaaaa
    57061 taaataagga aaatgataaa tgctttttaa ttgcatcaaa aaacgtacga tgctaaactt
    57121 ggtataagtt cgaaagttta aaggaaactt tcaagaacta aattacatta caaaggagat
    57181 gaattttatg acgtttacaa aaacaaatat gcaataccgc gtagcttttg atacaacttc
    57241 taaccgtttt atggcgattg atgcacaaaa cgaaaataac gttgcgtttg gtattacaat
    57301 cgaacaagca gtccgagcat taaacgaatc tagataagga gtgaaccatt ccaaatggcg
    57361 actgatgaga tctcggaaaa tcaataaaag acgtttgtaa cggtaatccg cgacaaacgt
    57421 ctttttgtat tcattacata tatttggaaa ttgtctctct ttatctctag acctaaacaa
    57481 aaacacaagt tctaatatca accatctcgt tttttatgac aattttgtta gctgtttcga
    57541 tgataatatt ccgttattat ctgtataata tttaatataa taaaaataaa aaaggagatc
    57601 tttcatgaaa aaaattttag ggttgatcgt tatcatattg attgctttta taggatggaa
    57661 aggttgggac tactatcagt ctacttatgt aggtaaagat tattatgcgg tagtcaaagc
    57721 tccaatgcct gcagaaactg atatcaaagc agataatggg gaagtaattg gaagaggttt
    57781 taaatacaac gtagatgctt atgctgaaaa cggtgaaaaa agacagttag atttcgatgt
    57841 cattacttct ggtgactctg ccaatggttc tgcttacccc gaaggaacga tcttgcagtt
    57901 gaaagcaagc gaaaagcgga tcatagagaa aaaagtaatt accagcgata aggtaccctc
    57961 atctgtaaaa gaaaaattag gcctatctta agctaagaat atatcgtaaa aagcactcga
    58021 agtgagtgct ttttttgttg attttagaaa acaaaaaaat agaatgggtc ttcgaagtca
    58081 ctttcctgtt cttccagtcc ttattagaag atatcaaaga ttttttaggt cataaagata
    58141 tttctactac acaagtctat gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc
    58201 aactagaaaa ctatatagaa gagcaaataa aaaagcactc aaattgagtg ctttttttta
    58261 agttcgctat cactaaaact atcactaatt ttatttagaa gcgaacatgt ttctatcact
    58321 gattttttaa atctagcgaa aaaaccttat cttacaggcg ttcgttcaat tctttggcaa
    58381 ggtcttcaaa gcctggtttg cctagaagtg cgaacatgtt tttcttgtaa gcttctacac
    58441 ctggttggtc gaatgggttg attccattca agtaacctga aatacctaca gcaatttcaa
    58501 agaagtacat tgtataaccc aatgtataag catccatttc tggaattttg actagcaagt
    58561 taggcacgtc accgtctgta tgagcaagca atgttccttc aaatgctttt gtatttacga
    58621 agtccacttc ttttccttgc aagtagccta gaccgtctaa atcttctgct tgttcaggaa
    58681 ttgtgattga tttacgaggt ttttcaacct tcacaactgt ttcaaaaata ttgcgacgtc
    58741 cttcttggat gaattgtcct aaagagtgca aatcagttga gaagtttgca cttgaaggat
    58801 agatgccttt ttggtctttt ccttcagatt caccatacaa ttgtttccac cattctgaga
    58861 agtattgcat tcctggttca tagttgatca acaactcagt tactttgcct ttacggtaaa
    58921 gaatgttacg cattgctgcg tattggtaag cttcgttttc ttctagtttg tcgcttgagt
    58981 atgctttgct tgcatcagcg gcaccttgca tcaatgcatc gatatctgca ccactaacag
    59041 cgattggtaa caaaccaaca gcagttaata cagagaaacg tccgccgatg tcatcaggaa
    59101 tcacgaaagt ttcccaacct tctgcatctg cttctacttt aactgcacct tttgctttat
    59161 ctgttgtagc gtagatacgt ttgtttgctt cttcttgtcc atatttctta acaagaagct
    59221 ctttgaatac acggaacgca atcgctggtt cagtagttgt tccagatttt gaaataacgt
    59281 ttactgaaaa gtcgcgatca ccgattactt cgatcaagtc agcaatatat gttgagctga
    59341 ttgaatttcc ggcaaagaag atttgtggtg cttttcttgt gttgctatct aataaattga
    59401 agaatgtgtg attcaagaag tcaatcgctg cacgagcgcc taaataagaa ccaccgatac
    59461 cgataacaac taatacttca gaatcagatt ggattttttt agctgcttct ttgatacgtg
    59521 caaattcttc tttgtcatag ttcgttggta aatcgatcca gccgcggaag tcgcttcctg
    59581 cgccagtacc ttcccgtaat tcgttgtggg ctgctgttac ttggctttgc atgtacccta
    59641 actcttgttc gttgacaaac ggtgctactt ttgaatagtc aaaatgaatg tgtgccattt
    59701 gttctcctcc ttataggttt actctacagg taatacttta cgtttttttt tattattttt
    59761 caagcaaata aacaaaatgc tgacaatatt tccaagaaag tttcattttt caaactaaac
    59821 cttctgctgc cattgctttg gcaacttttt caaatccagc aacatttgct ccaagaagca
    59881 gatttttttc gtcgccgtac tcggctgctg tttggcgaca cgcatggtaa atcgtttcca
    59941 tgatttgatc cagctgctca tccacttgct ctctttccca agtcaatcgc tgggcgttct
    60001 gactcatttc taaagcagat actgccactc ctccagcatt agcagctttt cctggacaat
    60061 accacacgcc tgcttcatga agaacagaaa cagcttttac tgtacatggc atattagcgc
    60121 cttctgctac gattttgatc ccattttcta ctaagatttc ggccaagctt tcgtctattt
    60181 cattttgagt agcacatggc aaagcgatat ccgctttttc tttcaatgtc caaacagatt
    60241 ctccagcatg ataaatagca gaaggacgtt ggtttgcgta ttccgtcaaa cgttgtctat
    60301 tgatttcttt gactcctttc aatagtttca catctattcc ttctggatca tagatgtacc
    60361 cgctagaatc tgaacatgta acaactttcc cgcctagctc ttgcactttt tcgattgcat
    60421 aaatggctac attcccgctt ccggaaacaa aaactgtttt ttgattaaat gaatcttttt
    60481 cttcgttcaa tagatgtttg acataataca ctaatccata accagtagct tccgtacgta
    60541 tcttacttcc ccaaaaatcc aaaggtttac ctgtcaacac accagtatca tattttttca
    60601 atcgtttata ttcgccgaac aaatagccga tttctctagc tcctacaccg atatctcccg
    60661 caggaacatc aatcgatggg ccgatatgtt ttgacaattc cagcatgaaa ctttgacaaa
    60721 aacgcatgat ctcattatca gattttcctt taggatcgaa gtcacttccg ccttttcctc
    60781 caccgattgg cagaccggtt aaactattct tgaaaatctg ttcaaaagcc aaaaatttta
    60841 agatacttag attgacacta ggatggaagc gcaatccacc tttataaggg ccgatagctg
    60901 aattgtattg gatacggtag cctctattta ctcgccagtt tccttcatca tcttgccaag
    60961 gcactcgaaa ttgaataact ctttctggtt ccgttaaaag ttctaagata ttcttttcga
    61021 tatattcggg atgagtgtcc aaaaaagaca taaccgtggg catgaattca tcgattgctt
    61081 gtaaaaattc ttcttgccct tgatcctttt gatgtagctt ttcttgaatt gcttgaacat
    61141 attctgttgc gtttgtcatc gttccacgct cctttgctta tcttccccca ttttagctca
    61201 ttttctcttt tggctcaaaa catttttttc tgttttcttt cttcagatat gctacacttt
    61261 ccttgaggtg agggaatgga aaaactgtat gatgtgatcg tagtcggcgc tggaacaagc
    61321 gggatgatgg cagcgatcag tgcagctgaa caaggcgcac gcgtactctt gatcgaaaaa
    61381 aacaaaaaag caggaaaaaa actactgatg acaggtggtg gtcggtgcaa tgttacgaac
    61441 aatcgtcctg ttgatgattt gattgcacat attcctggaa atggaaagtt tttatatagt
    61501 acgtttgcgc aatggaataa tttcgatatc atgaacttct ttgaatcaca aggcgtacat
    61561 ttaaaagaag aggatcatgg acggatgttt cctgtaacaa ataaatcaaa aacgattatc
    61621 gaagcattgc tcaatcgtct gaaagaacta gatgtgacgc ttctattctc tactcgagta
    61681 gagaaattga tccacaaaga acataaaatt tacggtatac gcacggaatt cgaagagttt
    61741 catgctcctg ccgttatctt aacaactggt ggacggactt atccatctac tggttctact
    61801 ggagatgggt acaagatcgt gaagcgagtg ggtcacacgg tgacgcctct ttatgcgaca
    61861 gagtccccct tgatatcgga tgaaccgtat attcaggaga aaacacttca agggctttct
    61921 ttacaagaca tcactttacg cgttttaaat aaaaaaggtc gtgtattgac tgaacatacg
    61981 atggatttac ttttcaccca tttcggtatt tctggtccgg ctgcactgcg ttgttcaagt
    62041 tttgtcaata aagaattaga gaaaacggga gaaccagtga cactttctct ggattgtttc
    62101 cctacacaaa ccaaacaaga gcttattcat ttattgacag aaaaaagtaa aaccaccaaa
    62161 aagaacttgg taaatgcctg gcacggttta ttacctgagc ggttgttagt tttctttctt
    62221 gagcggcttg aaatggatca tctgacagga caacaagcct ctcaaaaaca aattcaagat
    62281 ttcgttcaat tatgcaaaga gtttaagcta tcaatcaaca agacttttcc tattgagaaa
    62341 tcatttgtaa ctggcggagg ggtttctcta aaggaaattc atcctaaaac gttggagagt
    62401 aaaatcattg atggtctgta ttttgcggga gaattactgg atgtcaatgg ttatactggc
    62461 ggctttaata tcacagcggc tttcgctaca gggcatgtag caggaatgaa tgctgggcaa
    62521 agagcataat gtataaaaaa gaaagaagaa gactgggcaa acaacatccc ggcctccttc
    62581 tttcttttat acgtatgatt tattgtttta tatctgcaaa ttgtccgctc gttaatgcac
    62641 agatatcttg aggtgctaac tccatctgta gccctcttct gccagccgag acaatgattt
    62701 gttcatagct ttctgcaatt tgatctaaat aagtaggaaa tagttttttc atacctatag
    62761 gcgagcagcc accacgtatg tatccggtcg ttgcttccaa atcttttaaa tggagcattt
    62821 ctactttttt attccctgaa acttttgcta atttttttag attcaattcg gcttctcccg
    62881 gtatcacagc aacaagaggc cccgtcacat tgcccacagc aaccagtgtt ttaaatatac
    62941 gagattggct ttctggaagc tgttcagcga ccgatgacgc acttaggtgg ctttcgtccc
    63001 actcatattc gtgctctgta tagggaatct ttttttgttc gaccatacgg attgcattcg
    63061 tttttactat ttttttcttt gccaaaaaat atcacctcaa acttgttcta caacggttca
    63121 tttaataatt gaataatttg cttcaatgct ttgaccggta attgtcctgg agcagaagta
    63181 gaatcgctgc ttaaggaacc aaatgtcata acggaacccg tcgttccgcc agccagacga
    63241 gaaattttac caagatttcc catggacatc gtaataatag gcatggagac aaaagcattt
    63301 gcccgtctag tcaactccat caaccgcaaa acatctcgtt cattattggg agttactgcc
    63361 agttttccga tatcggctcc aaaatgttcc ataacattca atcggtaaag caagacaggg
    63421 tctgctggtg tttctttaaa atcatgacta ctgataatta aaggaatgtt taaagacttg
    63481 atttcctcta tcagtctttt tcctagactt tctactttga acaactcgat atcgaccata
    63541 tctactaatc ctgtgtgtat cacatgttca taaaggtcgc gatactgacg gatcgatatg
    63601 tcttgttctc cgccttcagc taaggtacga aacgtaaata ataacggcag tccatcaagc
    63661 atctcatgga taagaaccaa tgtttctgct acttcatcaa aagaaaaaac ttcacgataa
    63721 tgatcgactc gccattcgac taaatcggcc cctgcttcac gtgcaagaac tgcttcacgt
    63781 aatacttcct ctcggacggc cccgataaga ggcgcacaaa tctttggtct accgcttcct
    63841 atattcaatc cctttacccg aaccattctg catcgctcct tctaatggtt atttttaatt
    63901 tgataaacaa acactttcag ataattgcct tctaaaaagg tatccgtcgt ctggaaatct
    63961 tttggcagac gatataaagc aagttgttca aaagaaacat ttttggcatg gaattcctct
    64021 tcaatcattg tttggaattg ttctatcgag acattcgcag catttgtaga cgcaataagt
    64081 atcccgtctt ctgctaaaat atccagacta tccttgacca gttccccata atcttttgct
    64141 acgcgaaaga cttttttctt atttctggca aaacttggtg gatccaatac aatcatgtca
    64201 tataaaaatt gttttctttt ggcgtatttg aaatactcga atacgtccat aacaatgatt
    64261 ttttgctgat cgacatccaa accattcact tcaaactgtt cctgtgtttt ttttaaactg
    64321 cgttttgcta gatcgacact tgttgtttcg attgcccctc ccattgcggc agcaacagaa
    64381 aaggcgccgg tataactaaa catgttcaaa actgttttcc cagaagcgaa accttctgct
    64441 aatgatcccc gtacttcttt ttggtctaaa aagatcccag tcattaatcc ttcatttaaa
    64501 taaacggcaa atttgactcc gttttctttt acgatcaagg gttccggtgc ctcttttccg
    64561 taaatgaact ggctctcagg cagatttact gattcaaacc gaattttctc atagcctcct
    64621 atgatttccg ggtagatttc acgaaaagct tgaatcaata atccacgatg tgcatataac
    64681 gtttcgttat accaagaaaa aacagcatag cctgcataat aatcaataat cagaccgccg
    64741 atcccgtctc cttctccatt gaatatacga aaagcggtag tagaatcatc tgaaaaaaag
    64801 aaagttcttt tttcttttga cttcacaaaa agttcttcaa aaaaagattg atcaaatggg
    64861 atgttctgct ggctcaatac ccaaccgatt cccttattct gctttcctag atatcctttt
    64921 gcgataaatt ggttttgcga atcaactaat tccacccatt gcgtggtttc tttttgggga
    64981 tgagcaaggt cttctttttg aatcagtgga tatccttttt tcaatcgact gcttgctctt
    65041 ttttttactt gtactttcat tccttcacct gctttagctt tctcttccca agtataacaa
    65101 aaaacatagg aattgtctta ctgctcattt tttcatgaag cttaaagaaa accttaatga
    65161 aacagcataa acttctggtt tcaattgaca ctatattgtc atactagtaa aataaatgag
    65221 aataagtgag ctgtgtaaca tggtttcatg acacttctgt gattaagaag tgcagaaagg
    65281 aagattatct tgaaatatat aaaaacacct gcctttttga ataagaggct cggatttttt
    65341 tctttactag tcgttttact ttggttgaaa acgatttttg cctatttagt tgatttccat
    65401 ctcggaatcg aaagtggcat tcaatacttt atcttgttta tcaaccctat tgcaactaca
    65461 ttgttattac ttggaattgc tctttatatc agacgcccta gagttgctta tatcacgatg
    65521 atggtcattt attttctttt gacattgctt gttttttcga atgtcactta ttatcgagaa
    65581 ttcacggact tcattacgat caacacgatt ttaggtgctg ggaaagtagc gagtggtctg
    65641 ggagaaagtg ccattcgact gtttcgccca tatgatgtga tctattggat cgattttatt
    65701 atcttgactg ttctgctgat tgccaaaaaa atcaagttgg accctcgccc gattcgagca
    65761 agaatggctt ttgcgatctc tacattagca gttatgattt tctcaggaaa tctttttatg
    65821 gcggaagctg atcgtccaga attgctgact cgaacattct ctcgtgatta tttggtcaaa
    65881 tatcttggct tgaatgcttt catggtttat gacggtgttc aaacttacca gacaaaccaa
    65941 gtgcgggcag aagcaagtcc caatgatatg aaagaagtag aaaattatgt aaaagaacat
    66001 tatgcagcac caaatgacga tttgttcggg ctcgctaaag gaaaaaatgt gatttatatc
    66061 catttagaaa gcttccagca attcctgatc gattataaat tgacagatga gaatggtgtc
    66121 cagcatgaag ttacaccttt catcaatagt ctttaccaca gcaacagcac gttcagtttt
    66181 gacaactttt tccatcaagt aaaagcagga aaaacaagcg atgcggaaac gctgatggaa
    66241 aattcattgt ttggcttgaa tcaaggctcc ttgttcacac aattaggcgg aaaaaatact
    66301 ttccaagctg cacctgatat tttaagtcaa acacaaggtt acactagtgc ggtattccat
    66361 gggaacgctg gtacattctg gaatcgaaac gagacctata agaatttagg atatgattat
    66421 ttctttgatt cttcttatta tgatgttaat gatgacaatt cgttccaata cgggttgcat
    66481 gataaaccat ttttccaaca atccgtacaa tacttggaac gtctgcaaca accattttat
    66541 tcaaaattca ttgctgtatc taaccattat ccttattcga aattcaccaa tgacgaagct
    66601 ggattcccat tagccaatac aaacgatgaa acgatcaacg gttacttcgc gacagccaat
    66661 tatttagaca ctgccgtaca agaattcttc aactatttga aagaaagcgg cttgtatgat
    66721 aattcagtta tcgtccttta cggtgaccat tatggtgtct cgaattctag aaataaaaat
    66781 ctagctgaac tagttggaaa aacaagttct acttggacaa attatgacaa tgcacaaata
    66841 cagcgtgtcc cttatatgat ccatattcct ggacaaacac aaggcgggat caatcacact
    66901 tatggcggcc aagttgatgc tttgcctaca ctgctccatt tactaggtgt agatacaaaa
    66961 aattatattc agttaggaca agatctattc tcaaaagatc atgaacagct tgtttctttc
    67021 cgtgatggcg attttgttac tccaaaatat acttactacg gtggtaccat ctacagtaat
    67081 gaaactggtg aagccattac tgaacctact gaggaagtac aaaatgaaat tgattcactc
    67141 aaagaaaaag ttgataaaca gttagctgtt tctgatcaaa tcaataacgg tgatcttctt
    67201 cggttctata cgaatagtgg attagaccca attgatacca gtgattatga ctacaagaat
    67261 ggcttagaga aattaaaaga agctgaaaaa tctctaggtg acaaatcaac tagtgtcttt
    67321 agtcaacaca acggacaatc aactgttgat ctttataaaa cacaaacgta tcaagaactt
    67381 cacggaacga ccacaagttc ttccgaatga taaaataaat ttaaaaggag cagcgacaaa
    67441 attcttgtcg ctgctccttt tttcgaataa acagtgttca aaagcttgca tctacggtaa
    67501 tagaataggg cagcttcttc acaacctctt gctttttaca aaatattttc tggttttaat
    67561 tcgtgatttc actaaaggaa tttcatactt tattcatatt cattttctat actttttata
    67621 gaggtgaaaa gtatgaaaat aatccgctat gctactgctc gattaattta ttacaaaaag
    67681 cagacaatac tctattgttt attttcaagc ttttctgctt ttttattgat agcatgcctg
    67741 actctttacc atttgcaatc ggctttagcc aatcaagtcc aagatcggct ttcatttatt
    67801 ggcgcttctg catctacaga gcttcttccg gctttagaat tagctagccg attttatata
    67861 aaaggcatat tcagtttatt ttttctattt gccttccttt ttatcttttt cttttactat
    67921 tcagtcaggc aaaacaagca ggaattgatc aattggcgcc tgactggtct ttctaaaacc
    67981 aaattattcc tttttatttt ctggcagctg ttctttcctc tctttctttg ttgtatcttg
    68041 gttttattgt ggatcgtcag cttccagtca gtttatgaag cgcttcttca aaggatcaat
    68101 tttcttgtct taaagactac tgaactgcca gatatccgtg atatcttgac ttcggtaagc
    68161 ggactgacaa ttccgatgga tcaacagact ttttttacca tcgatttcca aaatgatctt
    68221 tttttcacag atatacttaa gggatttgcc caaactctta ttggactcag tggatgctgt
    68281 ctcattctta gtctattccc tttcactttt tttatccatc atttgcaaag taggaggcct
    68341 ggacgtaatg aaacctgaat atttgattca tgctgaacac ttgaccgtta ttttgtgtcc
    68401 tctcgaaaaa cagcataaaa tattggagca cttgaaaaaa gaggttcttg ttgcaaaact
    68461 ggataatttt gcgatcatcc aaaaaaattg gccgatgttt ccttatctga atcttaaaga
    68521 ccatgtcctg ttggatgtac cggaaaaaga aatcaaagaa gatcgtttgg cctatcaaga
    68581 aaaacttgat atctctcctt ccttgcttaa ttgtgcggca gctgagttga cgtcttttga
    68641 aaaagtaaag ctgcaattac ttcatgcctt gctttccaag cgaaacaata ttgttattga
    68701 agatacattt gatgaactta gtgtattgga gatccaagag ctactccact tgctttctta
    68761 tttagctcat gaagaaaatc aaggtatctt actctttact catgatgcta ctattgccca
    68821 aagtccttat attgatcgtt tagagcccgc gggctaaatc aatcatttta tagtaaaaac
    68881 aagcggtata gaagatcgat tcatgatctt ttataccgct tgttttttac tcttctttga
    68941 tttctacgtc aggcaaacga acagtgaatt ttgttccttt tcctagttcg ctttctactt
    69001 ctaattttcc tttatgcaag cgtacaagtt gctgcacgat cggtaaacct aatcctgatt
    69061 caccatattt tgtatttttt cttgaaggat ctactttata ataacgatcc cagatacttt
    69121 tcatttgttg ttcagacatt ccaattcccg tatcttcaat cgtgatgatc gtttcaagat
    69181 agcctttttc tagagtaatc gtgatttggc cgttttcagt aaactgtatc gcgttttgaa
    69241 taatattgac catgatttgg acaaatcggt cataatcagc gtaaacatca atatcatcaa
    69301 cggctttcaa tgtcaatgta tctcctgctg cttctgcttt agcttctaac tgtgcaacaa
    69361 tatttttcaa tgtttctgtt ccattgaatt ttttaatgac catactgatt tgattcgtac
    69421 ggattttttc ataatccaag ttttcattca ccaaacggat cagacgctct gtttcatttt
    69481 tcatcaattt aatcgcgttt tctcgctgat tttcagggat tgcattgtat tctaaccctt
    69541 ccagaagtcc gttgattgtc gtaagaggtg ttctcatctc gtgagaagca tctgccataa
    69601 attgtcttcg acgattttct tgttcttcaa tttctgcttg agattctttc aatgaattcg
    69661 tcattttatt gaaatcatct gctaactcat caaattcatc tttgtcatgt acggggagct
    69721 gtacatcaaa attaccattc gtcacttctt tcgtcgcatt ccttaaccga ttgatacgct
    69781 taacttggaa tgctgcaaaa gcatagctgg caatgactgc aacgatactg gaaaaaataa
    69841 atcctttgaa caggttcagt gtaattggac gaacgctgtt atcgatattt cgcgcaggtt
    69901 gactaactac aagtgctcca tagaattcat gattcaaatt aaatggcaca agtgcgtaag
    69961 aggtcgcctg tgcttgtcct aaaatatttt tattcgatgt gaatttttct tgacgtccat
    70021 tcctcagatt tttccattga tcagctgtta aggtaaaatt cagtaaagct gtattagtcg
    70081 gataaatgac acgttcgttt ttatcgataa atataaagtt cacattttgt tctgtcagta
    70141 cttgctctgt cagcagcagt gcattttgaa acgcctgatc ttgactaaac tgcgggagcg
    70201 tatcggcata ggtttgcgtt gtcttttcaa ctgattcagc atatccaaac agttgctggt
    70261 aattgttttc ttcaatcgtt tgttttgtca gttgtgtaaa agaaactcca acaatcaata
    70321 agatcaacgc gaccattccg caaaaagcta agagttgctg atacaaataa cgcattaggc
    70381 aacaccagaa tcatcgaatt tgtagccgac tccccaaaca gtttggatga cttgtggtcc
    70441 gaccttttcg attttttgtc gtaatttctt gatatgggcg tccactgtcc gttcgtcacc
    70501 aaagtactga taatcccaga ctaattcaag cagctgctcc cgagagaata cttgacgtgg
    70561 tttttttgct aaagtaaaca atagatcgaa ctctttaggc gtcagtcctt caattggccg
    70621 gttgtccaga tacgcttctc gtgttttcgt attcatcttg aaatgactag tctcgatatc
    70681 aaaatgatca tctgaaactg ctgcttcttc tcttgcttct gccaactcac tacggcgatg
    70741 caaagctttc attcgagcaa taagcgtcag aggactgaaa ggttttgtga cataatcatc
    70801 tgcccccatc tctaagccga tcacctgatc gctttcagaa tctctagctg tcagcatgat
    70861 aatcggcaca gtacttgaaa ctttgcggat ctctcttcct actagcatac cgtccagtgt
    70921 gggcaaattc aggtctagtg taatcatatc ccaatgttgt gggttttcta aaaaggcatc
    70981 gagcccttct tttccatcat acttgaaagt tgcttcccaa ccttcattta agaaaaacat
    71041 ctgcatcatt tcagaaacag attgattatc ttcaatcatt aaaatattca ttatcgttcc
    71101 ctccagtttc caggtcatat ttctgtaatt tcttttctgt cataataaaa ataggcataa
    71161 ttggaaatac tagcaaaagc atttcaatca gaaaataatt cgtatgccca gatgtcttta
    71221 aataatagcc gatcaatgcc atcagtaagc ccataagaaa aaaccagttt ccgcttgtct
    71281 tttgagccag ccgccaatga gcgtctgttt ttgcggcaag aggcgaatgg tacccataaa
    71341 aaggcagatt tccttttgag ggcagaatcc gaaaaagact gccaattatt aagaataaga
    71401 taccaatact gaaaaaaatc attctttccc tcctaaattc tcttcttata gtatactcta
    71461 aattgttgac tgaaccaata tgttcaggaa ttcttcacga aaggtttgtc ttttgttcat
    71521 tctttgttaa gataaactta ttccaataaa aaaggagttt tatttgtgtc aaattatcgt
    71581 gcaatcactt tctttgatct tgatggaaca ttactagatg cgaaatcaaa aatcacaccc
    71641 gaagtggcaa aagctatgaa cgcattaaaa gaaaacaatg ttctccccgt catcgctaca
    71701 ggacgtacag aagcagaaat ccactctatc atggaagatg cgggcattac ttctgcggtc
    71761 accatgaatg gatctttcat tcgagtagac ggcgaagaaa tctattctca aacattttct
    71821 tcagaagaat gtcaaaaaat gtatgatcat gtacagttga acggtcatga actttcttac
    71881 tataacgatc agaaaatctg gtgtaccggt catagccaaa caatgatcaa tgcttacgac
    71941 ttcattcatt cagatgttcc ggaaattgat cctttaggct ttaaagaaaa cacagtaaat
    72001 atgttgctga tcttatctca atcaggtgac gaatactatc acgaacattt tcctgagcta
    72061 actttttatc ggaatggtcc ttactcgatc gatacagtga agaaaggaat ctcaaaaggg
    72121 gcaggtgtca aacgtctgca aaaagaactt cagctggaaa aagttcctac ttttggattt
    72181 ggcgatggtc cgaatgattt tgccttgttg gaagcttgcg acaacaaaat cgctatgggc
    72241 aacgcctatg acggattaaa agaactttct acctttatca caaagaaaaa tacagaaggc
    72301 ggaattgtcc acgcattgaa gtattttgat ctgatttaag attaagatct tttttttgaa
    72361 gctacctctt ttcttaagaa aatcgcttat tttttcttaa gaaaggggca gttattatat
    72421 aaataatggg atatgatccg cttttttatt gaatttatcc caatatatgc tatactaatt
    72481 ttgtatgaaa aatacacgta acccttgtta gctaacgggg acgttacgga ttcgacaggc
    72541 acagtcgagc ttgaattgcg tttcgtaggt tacgtctacg taaaaacgtt acagttaaat
    72601 ataactgcta aaaacgaaaa caactcttac gctttagctg cctaaaaaca gttagcgtag
    72661 atcctctcgg catcgcccat gtgctcgagt aagggtccta actttagtgg gatacgtttc
    72721 aactttccgt ctgtaagttg aaaaagagaa catcagacta gcgatacaga atgcctgtca
    72781 ctcggcaagc tgtaaagcga atccttaaat gagttgacta tgaacgtaga tttttaagtg
    72841 gcgatgtgtt tggacgcggg ttcgactccc gccgtctcca ttgtgagatt tcaccagatt
    72901 tcgctagatg cttaaatcct tattttataa gggtttgagc attttttatt ttcgcagatt
    72961 ttcgttaaat gaatgaaaat ggcattcaaa atagcattca aaaaaacatt ttttaatata
    73021 tatgttccat ccatattgtc aacgctattc ctactttaca aaaagaacat tagttcgtat
    73081 gctcttctcg aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaaatt
    73141 cgatcctacg aatagattta tcaaagattt ttacaaagta tggaattcag aagttttctt
    73201 tttagcaatc gaagacaatt tattaatcaa tctctactat tctaataaaa actactttaa
    73261 gatccctgct gcgaaaacga gaatgaagaa ggatgtatac tttttgtttg atgtcgtgac
    73321 tgacgtgccg gacgcacgga gcgatcatcg gcgttatgat tatataaagt atactttcgt
    73381 tgatccagaa agatataaag attaagtatg taccgcccct catcgaggag ctatttttat
    73441 ttatagcgtc aaatagatat cagaaacccg cacatcattc accgcacctt catcattgca
    73501 gtgattcatt atatccttaa aactttttta tgatgaaata atattggaat atattcaaat
    73561 ttatagtatt ttagatatag aaaatctatg cgccaaattt attcatagaa aatattgggg
    73621 tgaactttgt tggtaaaaga aaatttagag ttagaagata ttcatcaaaa gagtaaagtt
    73681 attgcaaatg aagttatggt tactgcatct aaagcatctg tacctttaac ttctaatgaa
    73741 aaagcagata tagaacaagt attttcagaa aaaactattg aattaagtga acgagctgat
    73801 cgcattctaa aagaccagcc ttctcttagt gaaaacgaat tagctataaa actgattaaa
    73861 gaagatttaa aaaatgcctc tatgttttct cctatgaaac gcatattaaa aaaagcaatt
    73921 aaacatttgg aggaaaatta atgatttcca atttaaaaca tatcttacct ttactaaaaa
    73981 aaattattat ctttatctat ggacatcgtg aagaactaga gaaatatgca agttatactg
    74041 ctgaggtata cgtcgccgtc gcaagagctg ccaaacaacg acaaaaactc aaacaaaaat
    74101 agtaccgtcc ctcaatgagg ggctattttt tatcgattag gaatcgttaa cacgtattct
    74161 ggaaaacgaa tatcgactgt ttgaccattg atcgtatcgc cgttcggatc gttggttctt
    74221 cgcaacattg ggaatacttt tcttccagca aacctccgaa tgtccaatgt tccatctaat
    74281 ccaaatcgaa gtccactagg taatccgtaa gctgcattca cgtcatcgcg aacaatccct
    74341 gccgatctgc aacgccctac ttcgacattt ggattatctg catccatcca gaaaacgaat
    74401 ccgtacccta agctgggcgc tccgttctgt ggaacgagcc atgcgccaat ccgaaaaaca
    74461 cctaatttta attcattgaa tttttccaat ttaccaacgt aatttccgac ggttttcggc
    74521 gcacttgctg caatgatctc atcatgtgtt tgagctgtgt cagctagacg tcttcgaaca
    74581 ttgctcatat tcacccccgg gcattgatta gatccatgac cgctaaattc gttatgacct
    74641 aggacgtttg ccgaagttaa tccaaaacgg cgcattgcat ctcttgctct cacatcaaaa
    74701 gctcgttcct gcgcttcggt gaaagatccg ttccctacta aacaaatgtg gtaagttact
    74761 gtgttgtgat tacctacacc gttcgtgaca tcgttatctc tataacacaa ctgaactgtt
    74821 ccatctcgta aaataatctc atggtaccca cctgtacccc accctagcga accccgccag
    74881 tgattttgaa atgcccagac atcgccgcta attgtagctg aatgatggcg agcgatattt
    74941 cttacctacg agacacttcg ttttgcattg gttggtccta aaattctaga atctcctctt
    75001 aaatcttgaa tcgtagtcat aaatagccct cctaatttta tttatagaaa aagagcagtc
    75061 aagcgactgc tctcactttt ctttcttgat gacactgaca cctaaatcga ataacccacc
    75121 agcggcaagc cctgaaacgg ctcctgccca tgcatataaa acgactgcat cttgcaggaa
    75181 agataatgcg tataagacac caagaaaaat accagcaatc acattgatca ctggcaagat
    75241 tttgtaattg ttcacctgcg ttttgattaa ccccgtcacc gcgatcacta acggtgtgat
    75301 gattgatgct gcagttaaaa tttgttccat ctttattctc ctccgatttg tttaaatagt
    75361 gttttgattt gttcttcaat tgcaaccagt cgattgccat actcgctttg actttcttgc
    75421 agaccttgga tggtaccatt cagccgatcc aacgtttttc tcaaatcagt aaaaaccgca
    75481 tagaattttg ccacgccaaa aataactgta ctagcaagcg taagaatagc gatccattct
    75541 gcaattgtta tagataccgc actcatttgc cacctccaac tagaaaataa aaaaccacta
    75601 aaaatagtgg tttaaggctt taatgtatct gtttcaatca tttttgcata ccaagatttc
    75661 aattcttcaa atctttcctg tgtatttaca gaatatactt ggtaatagat tttggattta
    75721 ttcaactttt ctatcacttc ttttgtcgca aaatcatttg aaacagaaag catagcatgt
    75781 ttgtaatttt ttgctttttc aatttctttc tcaattgtac tgtttgaatc gaatagccaa
    75841 gagacgcacg cttctggata taacttgttg aaaaattctc gttctttctt atcgcttaag
    75901 acaaaaaatg ttttttcaaa catatcatgt gatttcactg tatggacgat attatccaca
    75961 aattctttat tcagccagtc tccttttgat ccatccacat tcaagattaa atcactttta
    76021 gaaataactt ttactgcttc atcgaaagta ggaactttta ctgttttatt tgagaattca
    76081 ggataatcag tagtatcaat tttaagattt tcaatttgtt ccgatgtcat tttttctaaa
    76141 ggacctgcgc cgtcagttgt tcggtctact gtgtcatcat gcatgagaaa catgtttcca
    76201 tcgctactca tgcgcggatc gatttcaatg gctccgtaat tcatatcgat tgctgcttca
    76261 agcgcttcaa tagtattttc tggtgccgct gtatgggctc ctctgtgtgc cgataaaata
    76321 gtttctttgt tgtttaacca attaaaccct gtggttaaat tacaagcaga aaataaaata
    76381 atgcttatac tcaaactagc taatttaaca aactttttca ctaatattct ccttaactca
    76441 ttattaacat tagtatagca aagacttttc atacaaaaca gttaattctt attttggaaa
    76501 aggatcacct gtataccaac gtccgtaaaa atacgcattt ccagaaacat tgcacccctt
    76561 tcttaataga tttgtttgca tcccttctag attcctgccg tctccattgt gagatttcag
    76621 cagatttcgc tagatgctta aatccttatt ttataagggt ttgagcattt tttatttttg
    76681 ctgattttcg ttaaatgaat gaaaatggca ttcaaaaaaa cacatttttt aatataaatt
    76741 acttcctcca tattatcaac gctattccta cttcacaaaa atactagttc gtatatcctt
    76801 tttgaggtga acattaagtt aatgaaaggt aaaatatagt tatggtttaa gtatgttcct
    76861 tcaaataagt ttttcaatta taaactctag agaaattaat tcatcctaac ataaaataat
    76921 tgttcactca aaagtttttc tagaacaatt aaaacagcag gcttcacatt cgctatacat
    76981 ttgtaagaag caataattct tgtactaaaa ggcataattg ctttatttat tttttgactt
    77041 ctcttcaaac acaagaagta gttgtgtaat aattgcagaa ataaggattc caataataga
    77101 tataaaattt gacatggaaa cgtctataac atttaaagaa taaagtgttg aagaaagtaa
    77161 actgattgaa ataatccctc cgtaaaaaat agtctgtgaa aatacccact ttttttcaga
    77221 agacgtactt cgtttggttc taaaacctat ccctgcattt ctactcttat caaaaaacca
    77281 aatatttaga agtaataata ttgctattat taagataaaa ttactgtaaa aatccaaaag
    77341 ctatcctcct agcaactatt taaatgccca aaaaaattac gtctagataa tatcatacct
    77401 acgttttcac ttataaattt tttcataaaa aaagcgttta gaagcataat cgttaataca
    77461 gatggtaacc aatgtaactg gtttgaactg ggatatcacc ctctccatat atgtattgct
    77521 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
    77581 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
    77641 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
    77701 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
    77761 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
    77821 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
    77881 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaacctttt acaaatgctt
    77941 tacgataaca agcctgagct aaatgatcct gatatcagtt ttgcattttt tgaaatgatt
    78001 ggatcggatg atctttttaa aactactagt ttgtctatcc gaaaatagga cagttctggc
    78061 agtataggtt tttatcgagt ttctccacca ggcctcgtta cagaaacaga ttctctaggc
    78121 gaaccttttg aataaatcaa aaatcatgaa caatcactgc caactaaata aagtgccttc
    78181 aaaagcttgg tcttctcgct tttgaaggca ctttatttaa atctttatga aactaaagta
    78241 tgttcatatt tgttgctgta gttatataga tattctgttg tttccaactc ataaacgtga
    78301 gtatattttg ccactacttt tccgcatttt tctaatattt cattgcgatc gacgtcatga
    78361 tattcgtcta cgcagatcac aacacaattt tctaatttac tgattacttc tccaaccact
    78421 ttttgtttca agccaatagg ttggcaaata tattttcttc ccaaaatgat agtttcgtta
    78481 ttcatatttc atccatcctt aatgtttttt tcattattca aaatgataat accattaaaa
    78541 tatgattatg aaaaccaata acacttgtga ttttataaaa aaataaaaaa accgaaaaac
    78601 agaaatgccc actgtctttc cggctctttt tattcttgat ttctcttctt ttttgtttca
    78661 atagacgatt ttagttatta ataaatgaaa aagcatttat attaaaattt gctaataaaa
    78721 gatctacata tttttctaga aatgcttgat cgatttcatc ataccctttc gttactccgc
    78781 aatcgatatc taaaacaccg attaaattac cttcttttac cattggcact acgatttctg
    78841 attgtgcggc tgaatcgcag gaaatgtaat ttgcatgggt tttgacatca tcaacgatca
    78901 atgtttcttg tttttcagct gactctccac aaactccttt tcccatcttg atacgtgtgc
    78961 aagaaactcg cccttggaac ggtcctaaaa tcaattcatt accatcgaat aaataatatc
    79021 ctgcaaagac agtctcaggc aaagtctcat tcaacaatgc agatgaattt gctaaatttg
    79081 ctaaccagtt atcttctgct tctaataatc cttgttgctg taataataaa atttcgtatg
    79141 catctttttt ctgtttttct gtccacatga taaaaaccgc tcctttttga tctatggaga
    79201 agattatagc tcagtttttt tgtaaacgct aggtttcctc ctcaaaaaaa cgagatacgt
    79261 caggtttatc accttgaacg catctcgctg tctttttcta ttctgcttgt tggatttctg
    79321 ctgctaaatc agcataatca atcgtttcgt gaacaaaagt cggatcatca actggaaagt
    79381 gctgtacaag ggacgagatg cggctaatct caacttttcc ttcagccact tcaaaatcca
    79441 ttacatgtcc gccaaatgta tgtgtatcat cgataaaatg caggtgaaat cccgctgcag
    79501 caactccttg gaataattca ggcgtaaaga acccgactaa tgttccttgg acattgcttt
    79561 gttggaattc tggttgagac tctgaaattt ttgctagtct tctgtaaggt ttttcttgct
    79621 tcggcatgat acggatgtgc atatttttga ataatccagt tatcttcact gccaaaaata
    79681 ggttttcccc ggcttctctt actaatatgt cttttttcaa ttgttctcca tctgcagttt
    79741 ttgagacaga aaaactagta tctggcgtaa aatctgtaat tgctgcatag ggggtaagtt
    79801 cacttccatc taattgggca actgagccat ctgatcttcc ttgataagct acgccatcta
    79861 aaaagatgac ttcaccatca agtccatgca atgttcccaa tcccagagtt cctttttcta
    79921 atatatgagc aatcgtttct gttccatcca tcaagccggc catcaaagct cctaatgtcc
    79981 cgtgctggta taagattttt tcagtcatgt cgacacttcc ttaatagaat tgatctggta
    80041 agattgtttc tcccaatttc tcattatcac ggtaatcaat cggaatatca ataattacgg
    80101 gtccttctgt cgcaaatcct tcttctaaag ctttttctaa ttcctctaca cttgtagctc
    80161 ggattccttt agcgccaaaa gcttctgcat atttcacaaa atcaactgga ccaaagtcta
    80221 cacctgacgc ccgttgatat ttcatttttt cttggaactc taccatattg tagtgtccat
    80281 cattccaaat caaatgaacg atattcaatt ttttacgtac agccgtctct aaatcctggg
    80341 cagagaataa aaatccccca tcgccggaaa ctgatacgat ttgtgtattt ggacgcacca
    80401 atgcagcaga aatcgcccat ggcaatgcaa caccaagcgt ttgcatacca ttactgaata
    80461 acaaatgacg aggttcataa cttctaaagt ggcgtgccat ccaaatataa tgactaccta
    80521 catctacggt aacagtcata tcatctgtta cttttgactg aagcgtgtta atgacttcta
    80581 aaggatgcaa gatacctgct tctccttttg attgaacaat gtcccgctcc gttaattttg
    80641 cttgaagaga tgctaaatat tcttttgcat cttctgacac ttgttgaggc tctaatgaac
    80701 ctgtcaataa atcaagagtg gctgagatat caccgatcaa ttctctttca ggctgcataa
    80761 aaggatcgat ttcagctggc gcttcatcga tcacaatgat ccgcgcatct ttttccgcat
    80821 tccagtttct tgcttcatat tcgattggat catagccaat tgcaataact agatcacttc
    80881 gtttcaaaag catatctcct ggttggtttc taaataaacc cacacgacca aagaaatgcg
    80941 cttctaattc acgtgagatc actccagctg cttggaatgt ctctacaaca ggcaatgctg
    81001 tcttttcaac taattgcctg atggctaacg tttctttttc acttgatcct cgcataccta
    81061 ctaatagtac cggtagtttt gcttctctaa tgcgttcagc taggtaacgg atatcatctg
    81121 cagaagcaga acctaattta ggatcgctca atggcttgat gacattacct tggacaggtg
    81181 catccactac atcttgcggg atactgataa agctcgcgcc tttttttgaa ctcttagcca
    81241 tacgataagc attggcaatc acttctgaaa ttgtttctgg atcttggatt tctgcactgt
    81301 attttgtgat tggctggaac agtgctgcat tatccatgct ttgatgggtc aacttcaata
    81361 gatcgcttcg ttttacttgt ccggcaattg ctaaaactgg gtcaccttct gctgtcgctg
    81421 ttaccaaacc tgtagctaag ttactagcac caggaccact tgttgcgata actactcctg
    81481 gctctcctgt aatacgtccg attgcttgtg ccataaatgc agcattttgt tcgtgacgag
    81541 tcacgattaa ttcaggacct tgatcctcta attcattaaa tacaccatcg atttttgcac
    81601 ctggtatacc gaatacataa tcaacgtgat gattaatcaa actttctact acagatttcg
    81661 aaccttgttt cattttttta gccattgaac atcgtccttt ttatggttta tttttaatta
    81721 ttagttagaa ataatcacaa ataagtgata cagtttctaa acctgtttca ttcagaagta
    81781 tagcacgttt tatttcattt gtttagccat atgtttcaaa aaatgaaaaa gaatgacagt
    81841 ttttacataa aaaactgtca ttcttaattg aaagtttcaa ccaatttcac aaaaggtcat
    81901 atataattca gcaacaatcg tgtctcatca atcagaaagt ttaatttctg tccatttttc
    81961 aaagtgacag taatcatctc accatcttct tcgattttat ctgcttttag aggaagatag
    82021 ttcaagtaat catctataat ttgttttttc cctcggttat tttgatcttc atcaaaataa
    82081 gataaccgta caactttttt gttcgtttca tcaatgattg ccgttccgta gcctgtttgc
    82141 gtgaggaatt ccatttgcca aaatacgctg tcctctgaga tcaaaacttc atagtcttga
    82201 acaattactg gctcttttgg catggatagc ttttgaaata gctgatttaa ttcctctcgc
    82261 agccgattga ccatatctaa ttgactctga tctgattctt ccataataga aaaagattgg
    82321 gcttggctca ttgctgtttc gatagaggga gaagaagctg aacttaaaga agagatatct
    82381 actgttcttt gttcttttgc agtaatacta ctcgctaaaa tatctggtaa aaaaaacaat
    82441 cctgcgctga ataccagaaa gaaagcagaa agaagatata atttaatttt catctgtttc
    82501 ccctcctaaa gcaaaatcga tcgtgatcat tgtccctgag tctagcgtac tttctatgtc
    82561 aaaagaacta ttatgcaatt cggttatttt ctttaccaaa gctaatccaa gtcctaaatg
    82621 tttcgttgtc cttgaaggat ttcctgtaaa aaattcatct gttactttag acaaattttc
    82681 tgctggtatc cctctcccat tatcggaaaa ctgcagaata taacgctttt ctttctttat
    82741 tcctttgacc actaattttc catcttctgc tatagcttct aatgaattcg ccagaatatt
    82801 cattgctagt aacttgaaaa gattgatgtc tagcaaaata ttggcaggtt ctagttcgat
    82861 ggtataagta aatccttgtt tcattccttt aagaaacttg tttagatcat tgctgatcat
    82921 gctggtcgat atttcttcaa gagagacatt tgttctttcc agttcaatca atttcaaaat
    82981 atcattggac aaatgtttta gccggttgct ttcttcaagg atataatcga cagattcttc
    83041 ggctgattgg tccaattttt cccatttcag catttgagca tagccaataa tggaagtcag
    83101 cggcgttcga atctcgtgag aaaagctaga aacaaataaa tcttgatttt cctttgcctg
    83161 acggagagtt tctaaatcgt agttcaattt atcagccata ttgttcaaac tgttccctaa
    83221 tttcccaatt tcgtcttgag tattgactat tgcccgttct ttcgtcttac cttctgtgat
    83281 atgctgggct acagaaatca gctgattgat gggcttcaag atcatgcttg ttagaaaata
    83341 agtgacggga ataatagcca aaagcaaaaa gagtaaaatc agccgaaaat agacaaggga
    83401 atctttcgcg atttcttcat agttctttgc ttgatggtaa gtagctaaac gataggtagt
    83461 gtcgggtatc tttgaagtcg aataaagtat ctttttgttt ttaacagtaa ccatatcact
    83521 gatttctaca atatgattag acaactcttt tggagaatcg tatttttcaa acaaagtagg
    83581 ctttgtacta aaaaccgttt tacctttttt gtctagaatc tcaaattctc cgattttggc
    83641 tgcagctacg aattcaccat tttttctcat ttcatctgtt gtttcatctc cgtctgtttt
    83701 cgtcgacaaa agcagtaaga gatttttctg ctggttgact agcacttctt tttcttgaga
    83761 aactgtgcgc tggtaaatcg catgcagtgt aaaaccactg accaccaaat aaatgagtaa
    83821 aaagaagaat aataatgaaa agaaaacttt gctcgtatat ttcaagttca cccctccaat
    83881 aggtaaccta caccataaat cgtacgcaag tcctgtccta attgcaattt atttctgatt
    83941 cgttggatgt gtaaatcaag cgttctggag ttgggttctt cttcccttcc ccagattttt
    84001 tgatagattt tttctcttgg caaaataatt gctttatttc taattaagta aagaagtaat
    84061 tcatattctt taggagtcaa agaaacagct tgatttcttg cagtgacggc tcttgttttt
    84121 tcattgattt cgatatactt ccatttcaat aatttatttg agctagattt tccgtttctt
    84181 cgaagaacaa cttccacccg tgccagcaat tcttctagat caaatggctt tgttatataa
    84241 tcttcagctc ctgaccttaa tccactcact cggtcggaaa tctggtcttt ggctgtcaag
    84301 aaaataacgg ggagatcata ctcacgcata tacgcaaata cttcataacc atcgatttca
    84361 ggaagcatga tatccaacaa cgccaaatca aattcctgct gctcgatgat atctgccgct
    84421 tcctttcctg tgaatactga atagaccgta tagccatttt tttctaatgt acgaactaaa
    84481 agtttattga ttgcaagatt atcttctact actagtatct tcatacagat cctcaccttc
    84541 tgccaataga gcaaatttta tccctaactt ataaatatac acgaatatca gtgaaaagaa
    84601 aaagatgagt acgatcaata aaaaagatgg actgtctatc ctggtaaatg cttcgatcaa
    84661 tctcatgagc gtttctttgc tgtcggtgaa gacgtctgta ctgttcaatc ttaaaaagcg
    84721 accgaagtaa atccctatgg cgttgataaa caagatgatg ctgacaacta accattgata
    84781 gacgcctatt ccgattttgt tgacgattat atcggtaact atccataagc tttcacaacc
    84841 caaaaatgtg gttggaaaga cagaaagaac gactagcaac aatttatacc agatttccat
    84901 ctccatactt atcatcatag ttgatggatc ataaggtgaa agcaaagaca gatggaataa
    84961 atcggtaaac aaataaaagt tattgggaaa aaacagtaac cagaataaga tcagcacgag
    85021 agagactaat ttggatctgc tggattttat ccacatcgct aattcaatcg ggatatatgc
    85081 cagaatcgta ttagtaaaaa ataaataaaa aacacttttt tcataaaaag aaagataaag
    85141 aacgagcagc aacaaaatgt aatatcctct aataaaatac atctgctttt gaatcattgg
    85201 acatctctcc ttttttaaac taaacgggtc ggtgatcatt gcgcccataa taattttctg
    85261 cttttgatgg attatctaat aaatcgctga tggtcgtaaa aaagtatatc cttctctttt
    85321 taattcatca atcagcacag gaatagcatc tacggtctca ggatagatat catgtagtaa
    85381 gataattgca ccgtcatata ctgtgctttt tacttgctgc agaatttttt ccttttgtct
    85441 agattgccaa tctttcgagt caacagacca ttcgatcatt gggcgattaa tgattcctgc
    85501 tccttttttt gtgatagagc catacggagg tcggacatat gctggctttt taccgattgc
    85561 tttcaaaata gcgttatctg tttgagtaat ttcagcactg atttcctggt cagatagacc
    85621 tgctaaatct ttatgtgaaa atgtatggct gccgatttga tgaccatctt gtacctcttt
    85681 tttcaaaagt tgttcgttct gggaaacatt ttctcctaga acaaaaaaag tggcatgtat
    85741 atctttttgt tttaaaactt ctaaaagttt cggcgttgtg gacggattag gcccgtcatc
    85801 aaaggtaaga gcaatggtct ttgatgattt cattgtatct tccgtttctt tatttttttc
    85861 attttttgtt tgctgttcct ttgtggtgat cgttggactg gtagaatttt gtatttctgt
    85921 ttgctcttgt ttcctggagc tttcagtatt ttctattgtt ttgtcttcgt tcgttgaggg
    85981 cgtgaagact ctgtctgcca tatagacgct tccccctgtg atccctatta gaaggatgat
    86041 tattccaaag gctatcaaac tttttttaat catattcctc tctcctgctt tactgtaatt
    86101 gctgactgat tcgtttgatt tctgcatcag tcattttttg gtaagatatg ccgtcaatta
    86161 tttcttcttc cccttttaat tgatccgtac tcattttaaa tttgttgttc ttcgttatat
    86221 ataatttcag caattctccc aaaggtaaat tggttttcat attgttgcct aaaacattga
    86281 tgattttgtc tactttaggt atggattcca gagtcaatag cttctgcata atcgctttga
    86341 tcactttttg ctgtcgaatt tgtctcccat agtcaccatt aggatcgtca tatctcattc
    86401 ttgaccaggc aagtacttct tctggagata acgtaaggga gccttttggg aaatcatagt
    86461 gatcaaagct gaaagcaaaa tcattgacca cttcaatatc gccaacgata cttagaatac
    86521 tctccagccc tttcatattc attgccatgt agtagggaat agtagtatca agcatactct
    86581 ctgtcgtttg aatagcagtt tgttctcctc cgtaagcata agcggcattc aatttgtcaa
    86641 atctgttttg tcctgcgatt tgaacatagg tatctcgctc tagactaatc aattttattg
    86701 cttcattttt cagatcaaca tgagcagcaa tcattgaatc cgatcttcct cgttccgtcc
    86761 ttccaaattc tccagtatct acacctaata gcagtacatt gaaccgatcg ggtaattgtg
    86821 tgtctgtatc ggctgaatat ttcacattta tgttggcggc tgtttttttt gaaacctcag
    86881 acaggttttg caatttcata tattcgctta caccataagc acctgctgct ataataaaca
    86941 tcaatagaaa aatagatatg atttttaacg ttttatacat ttatcttctc ctcctttgct
    87001 gttaaggata gaagcaaaat ctatctgatt tgtatctaaa taccatactt tagagaaatt
    87061 aaaaaaaggt tgtgccaaaa ttcttgtcac aacctcttga taaacggtat tctaaaagta
    87121 gctttttcga aaaataattt atttactgaa ctggccgatg gtcatttctg ccataatagt
    87181 tctctacagc cgttggatgc cctaataaat cgccaactgt cgtgatgcga ta
//
LOCUS       DACCKU010000002        69415 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.002, whole genome shotgun sequence.
ACCESSION   DACCKU010000002 DACCKU010000000
VERSION     DACCKU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 69415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.002"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(524..751)
                     /locus_tag="IVN13_000091"
     CDS             complement(524..751)
                     /locus_tag="IVN13_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7010617.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            complement(760..1233)
                     /locus_tag="IVN13_000092"
     CDS             complement(760..1233)
                     /locus_tag="IVN13_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP7010618.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            2463..3029
                     /gene="amaP"
                     /locus_tag="IVN13_000093"
     CDS             2463..3029
                     /gene="amaP"
                     /locus_tag="IVN13_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7010619.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            3042..3230
                     /locus_tag="IVN13_000094"
     CDS             3042..3230
                     /locus_tag="IVN13_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7010620.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            3243..3800
                     /locus_tag="IVN13_000095"
     CDS             3243..3800
                     /locus_tag="IVN13_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7010621.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            3832..4074
                     /locus_tag="IVN13_000096"
     CDS             3832..4074
                     /locus_tag="IVN13_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7010622.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            4415..5629
                     /locus_tag="IVN13_000097"
     CDS             4415..5629
                     /locus_tag="IVN13_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP7010623.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            5660..7087
                     /locus_tag="IVN13_000098"
     CDS             5660..7087
                     /locus_tag="IVN13_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7010624.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            7110..8036
                     /gene="glsA"
                     /locus_tag="IVN13_000099"
     CDS             7110..8036
                     /gene="glsA"
                     /locus_tag="IVN13_000099"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP7010625.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            8244..9704
                     /locus_tag="IVN13_000100"
     CDS             8244..9704
                     /locus_tag="IVN13_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010626.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9925..11688
                     /locus_tag="IVN13_000101"
     CDS             9925..11688
                     /locus_tag="IVN13_000101"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP7010627.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11772..12230
                     /locus_tag="IVN13_000102"
     CDS             11772..12230
                     /locus_tag="IVN13_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP7010628.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            12339..13022
                     /locus_tag="IVN13_000103"
     CDS             12339..13022
                     /locus_tag="IVN13_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP7010629.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            13169..13783
                     /locus_tag="IVN13_000104"
     CDS             13169..13783
                     /locus_tag="IVN13_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP7010630.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVLALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13799..14005
                     /locus_tag="IVN13_000105"
     CDS             13799..14005
                     /locus_tag="IVN13_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010631.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            14546..15676
                     /locus_tag="IVN13_000106"
     CDS             14546..15676
                     /locus_tag="IVN13_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7010632.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            15768..16523
                     /locus_tag="IVN13_000107"
     CDS             15768..16523
                     /locus_tag="IVN13_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7010633.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            16581..18620
                     /locus_tag="IVN13_000108"
     CDS             16581..18620
                     /locus_tag="IVN13_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP7010634.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSYQATRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(18729..19880)
                     /locus_tag="IVN13_000109"
     CDS             complement(18729..19880)
                     /locus_tag="IVN13_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7010635.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(20034..20564)
                     /locus_tag="IVN13_000110"
     CDS             complement(20034..20564)
                     /locus_tag="IVN13_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010636.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(20663..21013)
                     /locus_tag="IVN13_000111"
     CDS             complement(20663..21013)
                     /locus_tag="IVN13_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP7010637.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(21170..22699)
                     /gene="ahpF"
                     /locus_tag="IVN13_000112"
     CDS             complement(21170..22699)
                     /gene="ahpF"
                     /locus_tag="IVN13_000112"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP7010638.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(22709..23272)
                     /gene="ahpC"
                     /locus_tag="IVN13_000113"
     CDS             complement(22709..23272)
                     /gene="ahpC"
                     /locus_tag="IVN13_000113"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP7010639.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(23416..24078)
                     /locus_tag="IVN13_000114"
     CDS             complement(23416..24078)
                     /locus_tag="IVN13_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7010640.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            24262..25908
                     /locus_tag="IVN13_000115"
     CDS             24262..25908
                     /locus_tag="IVN13_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7010641.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            25899..26981
                     /gene="uxuA"
                     /locus_tag="IVN13_000116"
     CDS             25899..26981
                     /gene="uxuA"
                     /locus_tag="IVN13_000116"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7010642.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(27089..28642)
                     /locus_tag="IVN13_000117"
                     /pseudo
     CDS             complement(27089..28642)
                     /locus_tag="IVN13_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            28790..29931
                     /locus_tag="IVN13_000118"
                     /pseudo
     CDS             28790..29931
                     /locus_tag="IVN13_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(30057..31136)
                     /locus_tag="IVN13_000119"
     CDS             complement(30057..31136)
                     /locus_tag="IVN13_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7010643.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(31239..31679)
                     /locus_tag="IVN13_000120"
     CDS             complement(31239..31679)
                     /locus_tag="IVN13_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7010644.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(31736..32146)
                     /locus_tag="IVN13_000121"
     CDS             complement(31736..32146)
                     /locus_tag="IVN13_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7010645.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(32326..32760)
                     /locus_tag="IVN13_000122"
     CDS             complement(32326..32760)
                     /locus_tag="IVN13_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7010646.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(32860..33708)
                     /locus_tag="IVN13_000123"
     CDS             complement(32860..33708)
                     /locus_tag="IVN13_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7010647.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            34097..35863
                     /locus_tag="IVN13_000124"
     CDS             34097..35863
                     /locus_tag="IVN13_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP7010648.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            35965..37224
                     /locus_tag="IVN13_000125"
     CDS             35965..37224
                     /locus_tag="IVN13_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7010649.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            37371..38672
                     /locus_tag="IVN13_000126"
     CDS             37371..38672
                     /locus_tag="IVN13_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7010650.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            38675..39526
                     /locus_tag="IVN13_000127"
     CDS             38675..39526
                     /locus_tag="IVN13_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7010651.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            39539..40354
                     /locus_tag="IVN13_000128"
     CDS             39539..40354
                     /locus_tag="IVN13_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP7010652.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            40505..41671
                     /locus_tag="IVN13_000129"
     CDS             40505..41671
                     /locus_tag="IVN13_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7010653.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            41901..42548
                     /locus_tag="IVN13_000130"
     CDS             41901..42548
                     /locus_tag="IVN13_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP7010654.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            42632..43576
                     /locus_tag="IVN13_000131"
     CDS             42632..43576
                     /locus_tag="IVN13_000131"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP7010655.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            43711..45162
                     /locus_tag="IVN13_000132"
     CDS             43711..45162
                     /locus_tag="IVN13_000132"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP7010656.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            45183..46019
                     /locus_tag="IVN13_000133"
     CDS             45183..46019
                     /locus_tag="IVN13_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7010657.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            46019..46678
                     /locus_tag="IVN13_000134"
     CDS             46019..46678
                     /locus_tag="IVN13_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7010658.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            46804..47493
                     /locus_tag="IVN13_000135"
     CDS             46804..47493
                     /locus_tag="IVN13_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010659.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            47499..48455
                     /locus_tag="IVN13_000136"
     CDS             47499..48455
                     /locus_tag="IVN13_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP7010660.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            48499..49011
                     /locus_tag="IVN13_000137"
     CDS             48499..49011
                     /locus_tag="IVN13_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7010661.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(49267..49815)
                     /locus_tag="IVN13_000138"
     CDS             complement(49267..49815)
                     /locus_tag="IVN13_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7010662.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(49904..51085)
                     /locus_tag="IVN13_000139"
     CDS             complement(49904..51085)
                     /locus_tag="IVN13_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP7010663.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(51321..52829)
                     /locus_tag="IVN13_000140"
     CDS             complement(51321..52829)
                     /locus_tag="IVN13_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP7010664.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            52988..53179
                     /locus_tag="IVN13_000141"
     CDS             52988..53179
                     /locus_tag="IVN13_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010665.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            53382..54299
                     /locus_tag="IVN13_000142"
     CDS             53382..54299
                     /locus_tag="IVN13_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP7010666.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            54292..55365
                     /locus_tag="IVN13_000143"
     CDS             54292..55365
                     /locus_tag="IVN13_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010667.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            55513..56031
                     /locus_tag="IVN13_000144"
     CDS             55513..56031
                     /locus_tag="IVN13_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010668.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            56012..56404
                     /locus_tag="IVN13_000145"
     CDS             56012..56404
                     /locus_tag="IVN13_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP7010669.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            56480..57415
                     /locus_tag="IVN13_000146"
     CDS             56480..57415
                     /locus_tag="IVN13_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP7010670.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            57548..58327
                     /locus_tag="IVN13_000147"
     CDS             57548..58327
                     /locus_tag="IVN13_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP7010671.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            58320..58637
                     /locus_tag="IVN13_000148"
     CDS             58320..58637
                     /locus_tag="IVN13_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7010672.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            58881..60491
                     /locus_tag="IVN13_000149"
     CDS             58881..60491
                     /locus_tag="IVN13_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7010673.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            60648..61496
                     /locus_tag="IVN13_000150"
     CDS             60648..61496
                     /locus_tag="IVN13_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7010674.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            61727..62206
                     /locus_tag="IVN13_000151"
     CDS             61727..62206
                     /locus_tag="IVN13_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010675.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            62239..64467
                     /locus_tag="IVN13_000152"
     CDS             62239..64467
                     /locus_tag="IVN13_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7010676.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            64596..66239
                     /locus_tag="IVN13_000153"
     CDS             64596..66239
                     /locus_tag="IVN13_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7010677.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(66312..66857)
                     /locus_tag="IVN13_000154"
     CDS             complement(66312..66857)
                     /locus_tag="IVN13_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP7010678.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(66880..67365)
                     /locus_tag="IVN13_000155"
     CDS             complement(66880..67365)
                     /locus_tag="IVN13_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7010679.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(67355..68167)
                     /locus_tag="IVN13_000156"
     CDS             complement(67355..68167)
                     /locus_tag="IVN13_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP7010680.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(68306..>69415)
                     /locus_tag="IVN13_000157"
     CDS             complement(68306..>69415)
                     /locus_tag="IVN13_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7010681.1"
                     /translation="PILYLMEQPALSVSQSFLDKNEISYMTHTSGTTGIPKLICHSAH
                     SMGWRTKWQKTVFTKISEKKLIAFHISPVHSRFNIGISSLMSMGFPMMPLANAQSSKV
                     VHMLEAHRPIALETHPNNFVQWRFTAKEHPEAFSGIRYYHSTFDAINNQTMKTFLRTS
                     MAQDAVFLQVYGQSECGPMILKAHTLESLKTSDARDMGVGLEDMTSARITDSVGNVLP
                     ENTDGHIQLLSKGRALTYYKEDARFQENVYGDWWDSGDYGFMDKHGHLFLKDRQVDLI
                     ETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSPQPIIALAPDKEMDWNRWWEQVHELP
                     RLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQKS"
BASE COUNT        23155 a        12290 c        13508 g        20462 t
ORIGIN      
        1 cattattatc aaaataatgt taaaaataaa aaagaattgt ccatcactgt tattcaaatc
       61 attttatcaa aactacatag taaatgttaa aaagcaacat gacataatat atagaaggct
      121 ttacagtata tatgggagct tactaaaaaa gaatggagta agagttaaca caatgggcca
      181 ttagctgaag ttttaagcac ttcaaggtat gaaacagaat ggcctgtaat actacatgat
      241 tatttagact gctcaaactg ctatcagtct ggtggaatga ataaagtatt atactaaaag
      301 cagatccaac tacaacgtaa caggaaacca gttgtatcag gaaaagtgat agtacatgga
      361 gaagaaaagg ccccctttgt atgagtcagt acatatctta tttatagata aaacggtatt
      421 caatcgaaat aaaaaatctg aataatagac gtaaataact tctaaaagtc tcacacatct
      481 atcattcaga ttatttttta tttactgttt gttctacttt attttattct tctggtatag
      541 tattcgagtc atttttatag gtcaacaaat cacctggttg acactccaaa acttggcaga
      601 ttttttcgag agtggaaaaa cggatggctt ttgcttttcc attttttaga atagagatgt
      661 ttgccatcgt aatgcccact ttctcactca attcagtcac actcattttt ttttgagcta
      721 atactacatc taaattaacg ataattgcca tgtcgcacct cacactgtca aatcatgatc
      781 tgtcttaagt aaaatcgcat gttcaagcag tttttccagc acgcttgtta atgtgtatac
      841 tgcaaaagga atgaatacga cagcgattcc taccaacatc aaacctggag catcgtctaa
      901 atccgctact ttcataaatt taggaagtaa gccaaaaaag gcgaaaccaa taaacaaaat
      961 ggttcgttcg atttttttta gtatgtttaa gctttgagtg gaaaaagctt ggttatcttc
     1021 gatttttttc aatagtttca ataagaaata agcaataacg atccctagga gaagggcaaa
     1081 gtaaatgaca gaaacaaata atgtggtctg ccaatcaaaa ttgaatcctt tttcgcttgt
     1141 agcgatctga gtataaaaaa gtacgccgaa caaaataaat acggcactta tacctaaaac
     1201 gacaaatttt aatagcaatg ttttaaattt catttttatc cctcttttat ctttgatagc
     1261 taaaatatag cagaataatt attgtaaaac aactattttt tatcgaaaaa caataaaaat
     1321 atatcgttta taaaaaatga tagagtatga aggtgtggag tgcatttaac agcttttttt
     1381 gattgtaaga aacatttaaa accagttatt ttagattcta tgtgtgtctc ttctataatg
     1441 ctagtttaga tgcctgggat tatattggac aatacagttg gaggattttt tattatctag
     1501 cgaagagaag tgtaagttaa cagaactgta tgagaaaaaa gacaactacg tagtagctaa
     1561 ccgattcatt aattttttca agttcatgtt tatttacgta tcagaatttt tcttattcaa
     1621 tggtttggtg taatcaccac ttatttgtcc tttattgctt atacaatgtt atacaaaagg
     1681 aaaatcaagc attgacgcat ggggtggtac tatctagctg aaatagctta ataagcgcaa
     1741 gtagatgcat tggattagtt tataaatcaa aagtgttaga ggtattaaga agaagtagaa
     1801 aaataaaatg gattgccgaa taaaaagtac cataaatcct aaaacatgtg ccaacagata
     1861 gatggcacat gtttattttt ttcgataatt ttgtaacaaa aatacgaaga tgaaaaagaa
     1921 tagagattcc cataatgtag tagtccaact gaattcttta ctgataccaa ataccatatt
     1981 tcctagattt gatacaatca aatagccaat cgttatgatc aatgaatagt acaatctttt
     2041 catcatagga catacctcat ttttatttat aagaatagta taaaccaaag aagtgaataa
     2101 gaaaaatgat aatataaaaa taaagtaaga acgcattaaa gtaactaaag ttatttattg
     2161 tattttttaa ttctccgaaa aaatttaata aataaataga atcttgtaat aataactgag
     2221 cataatcttt gcaattttta atcttgaata atatccttta agtatagcca gcaatcaaaa
     2281 gtaaatagag aataagtaag caattgttat ttcagtatca gactggcaat aaaacgccgt
     2341 tatgatccag caatgtttgg tagcgcttta tttactgatg ctatgctaac tgttcgctat
     2401 ctatactgtg attgtcggta gagttattta cagggtaaaa ataagtttat aggaggtgga
     2461 aaatgaataa ggggttaaaa ataatcggtg ttatcatgag tttactgcta ctttcagtat
     2521 ttctagcgat tgcgttgatc aatgcgccgt atattatgcc tgaacaattg gaacgatttc
     2581 gcttctttac tttaacgaat tactacatgc aacaatatat tttctgggca gctgtagtat
     2641 ttgccgttct tgctattatc atcttgctag tcgttctttt ctatccaaag tcaagaggaa
     2701 ctttcgttat gaaacgggaa gatggcaaac taacaatcga caaaaaagcg atcgaagggt
     2761 tggtacgttc ccatttacac gaagaagagt tcattcattc acctaaagta cggatacgtt
     2821 caacgaaaaa tcgaatccat atccatgtca acggagactt gaaacgaact tcttcattag
     2881 tagggaaaac aggtgtattg atgcaagata tcgaagaaga agtaactaaa gtactcggaa
     2941 caaaagaaac ggtcaaagtg gcagtcacat acagcggtta tgaagaacaa gaagatacaa
     3001 gagactacaa gcactctcga gtggaatagg agggccaaat aatgaaagaa ctattttcag
     3061 catacaagct ccctattatc tttgggggtc ttggactact gctagctgtt ttatggttaa
     3121 cgattggatt tttcaagaca ttgttgatca taatcatgac agcaattggc gcaggaatcg
     3181 gtttttattt gaaacagtca ggaatagttg atcaattttt taaatcatag gaggaaacaa
     3241 agatggaaaa caaaaacatg aatacaacaa atggatcagt tttggcaaat gaaaaaggag
     3301 aaaaccgcat gatgaatggt gaattgactt atgaagacaa agtcatccaa aaaatcatcg
     3361 gttatgcatt gaaagatgta gatggtctat tgacattaga tggcggattt ttctcaaata
     3421 tggctgaaaa attagtcaac acagataacg taacagcagg tatcaataca gaagtaggta
     3481 agaaacaagt agctgtggat ctagatgtga tcgtagaata cggtaaagac atcgaagata
     3541 tttacaacca aatcaaagaa ttgatcagta ctgaagtaaa caatatgaca catttagatg
     3601 ttatcgaagt gaatgtgaat gttgcagaca tcaaaacaca agaagaatac caaaaagatc
     3661 aagaaacagt acaagacaaa gtaacagaag cagcaaaatc cacaggacaa tttgcttcac
     3721 gccaaacaga taaagcaaaa tctgctgtag gcaaaggtgc acaaaaagtc aaagaaaata
     3781 acgaaccacg tgtacaataa gaagtaagaa gtcagttagg aggaatttaa aatgggattt
     3841 atttgggcat taatcgtcgg cggtatcatt ggagcaattg caggagcaat cacgaataaa
     3901 ggatcatcaa tggggatcat cgcaaacatc attgctggtt tagtaggttc agcaattgga
     3961 caagctttac taggaacttg gggaccaaca attgcaggaa tggctatcat tccatcattg
     4021 atcggtgcaa tcattttagt attagtcgtt tcattctttg cacgtaaatt ataagcaaat
     4081 tgaagaaaaa cttatgttga aatgaagaac aaatttgaga accgtagcga cactcgctgc
     4141 ggttcttttt tatatatttt atatgaagta ttctttattc cttatgaaaa aatatcttta
     4201 aaaataaaaa aagacgaaaa taaacggttt tctataaaaa gaagaccgtt tattttttgt
     4261 ctttatttgt gtgaaaaacg aaaaagagca caacacatca agcttttatt aattgtctgt
     4321 cttttataaa tgcaagtatt aattcaaaaa aacagaagaa aaaaacaact acaccttctt
     4381 ttatactcgt aatcgtagaa aggaggcgaa aaaaatgaat acattaccta tcaatacagg
     4441 agatacagca ttcatgattt tatgtacagc aatggtttgc ctgatgactc ctggtctagc
     4501 ttttttttac ggcggattag cacggaagaa taatatacta acgatcatgt ctcaatcttt
     4561 tctctccgtt gggataacaa cgattatgtg ggtatttggt ggattcggat tggcctttgg
     4621 acgggatatc ggcggtgtta ttggaaaccc ggcagatttc tttttgatga gacatgttac
     4681 aagcttcccc aacatgtttc atggtgcaac gattccattt ttaatgttct ttctttacca
     4741 actgatgttt atcgtgatta ccgttccttt gatgacagga gcttttgcag ggagattgaa
     4801 tttaaaaggc tatattatac ttgttatttt ttggaatctg ctgatttatt tccctgtttg
     4861 ccactggata tggggtggcg gatttttgga tcagatggga tttagagatt tcgctggtgg
     4921 agcagtcatc cacacgacag ctggattcgg ctctttagca tgtatcctca cacttggtca
     4981 aagaaagatt cctttaggag aaagaagtca gcatagcagt cttatggcag ctgctatcgg
     5041 tacaggactg ctttggtttg gctggttcgg ttttaattcc ggtggtgcac ttcgagcaga
     5101 tgaacaagca gtcaatgcat ttggttcaac attcatcgct cttgcatttg cgatgattac
     5161 ttggctgatc attgcaaaag taaaaggaaa tggctttgat tttgtcgacg tactaacagg
     5221 atccgttgct ggattggcag ccattacgcc ttgtgccggt tatgtagaag caaaaagtgc
     5281 catgctgatt gggatcattg cgggtatcgt ctgtcatgct gctgttgatt ttcgtaaaaa
     5341 gaaacaatgg gatgatgcac ttgatgtgtg gggtgttcat ggcatgggcg gattcacagg
     5401 tacgattctg atagggatct ttgcatctaa cagccattta ctaacgaatc caagcagctg
     5461 gtacttctta gggattcaag tgattggtgt catcattact gccgtgtatg cctatgtgct
     5521 gacaacagtg attttaaaat cggcgatgca ctttacaacg atcactacta caaaagaaga
     5581 acaagaagaa ggattggact ttttcttctt tggcaacaaa ctcaattgaa acaaattcaa
     5641 atagaaaaga ggaagattca tgcaacaaag caaaacgaaa attcctgcaa aaaaattaac
     5701 gttctttggc tttttggcta tgaccatctc aatggtcgtt tcattatatg aatatcccac
     5761 attcgcgacc tcaggcttca gcttagtatt tttcttgtta ttaggcggtt tgctatggtt
     5821 tatccctgtg gcattgtgcg cagcagagat ggcaacggtg aaaggatggg aaaaaggcgg
     5881 tgtctatact tgggtcagtc gcacattggg aaaacgattc ggttttgccg caatcttttt
     5941 ccaatggttt gagataacag ttggctactt gaccatgctg tatttcttaa ctggtgcatt
     6001 atcctatgca acgggtattg gtgctataca aaacaataaa tatctgaaat tggcaatcct
     6061 attgattatt ttctgggcta ttttactctc acagttaaaa ggaaccaaat acacttcatt
     6121 gattgctcgg atcggttttg tagctggtat cttgttgcca gcattagtct tgtttggctt
     6181 agggatccat tatgtcgctt caggcgctcc actacaaacg atactgtcta tgaaaacatt
     6241 gattcctgat ttttcgaaat tgcctacgct ggttgttttt gtttccttta ttttagcata
     6301 tatgggcgta gaaacatctg ccagtcatgc aaatgaaatg gaaaacccta aaaaggatta
     6361 tccattagca atgtttgtat tggttattct ggcaatcatt ttagatactt ttggcggatt
     6421 gaccgtcgct actacagtac ctcaacatgg tttgtctctt aatacagggg tgattcaatc
     6481 gttagaattc ctattaagac acattgatcc aaatcttgca tggattgcga aaattcttgc
     6541 gattcttgtt tgtttaggtg tcatcggaga aattgcttct tgggtaacct ctccttcaaa
     6601 agcacttcac gtagcagcag aagaaggatt acttcctgaa tattttgcaa aagaaaacac
     6661 acacggtgtt cctgctcatt tgatgatcgc aaatgggatc gttgcaacaa tctgggcagc
     6721 cgtctttaca ttaagcggcg gggggaataa tctttcattt cttgcagcga tgtcattgac
     6781 tgttgtaatc tacctgatgt tgtactttat gtttttcgcg ggctatttga agctggtttt
     6841 taaagatgac agtcttgaaa gagcctatca ggttccaggc ggaaaagttg taaaagtaat
     6901 cgttgcttgt ttaggtcttt tgacttcact ttttgctttt tgtatctcat ttgttccacc
     6961 tgctagtatc gcagctggac agcatgggat ttatgaaacg attctgattg tttcttatct
     7021 tatcgtctta gttattccat ttacgatcta cgcatgccgt cataaattcc agcatgcaga
     7081 aaaataaaaa atgaaagctg gttattcaaa tggaagtaaa agaattagaa caattgattg
     7141 gaaaaaattt ccactattat caaacaggac aagtggcgga ttacattcca gcattagctg
     7201 aagtggatcc caaacaatta ggtatggcga tttatgattt ggatacaaaa caactgattg
     7261 agggaggaga cgccgatgtg cgctttgcca tcgaaagcat gtcaaaagtt cccgtattgt
     7321 tattagcaat caaagacaac ggaatagaaa aagtctttca aacaatcaac acggaaccaa
     7381 ccggttttgc cttcaattct atcatgaata tggaaatcaa tcatcgcaaa catccaatga
     7441 atccttttgt taatgcagga gcgattgcaa caacctcgtt gattgccggt aaagatgcag
     7501 atgaaaaatt tgagcgaatc ttgtcattta tgaaagagat ctgtgatgat cctaatatca
     7561 cattgaatga agaaatctat cattctgaat caagaacagg tgatatcaat cgctctcttg
     7621 cttactatat gaaaggtaat caaatgatcg aaggagacgt accggagata ctcgatgctt
     7681 atttcagaca atgctccctc aatgtcaccg ctacaggcat agcaaaatta gctgctgtgc
     7741 tggcaaataa aggaattgct ccttggaatg gcaaacaaat cattactgaa gaaagtgcta
     7801 caatcgtcaa atcaattatg acaactgctg gtttgtatga tgaatcagga gagttttccg
     7861 tccatgttgg tgtacctgca aaaagcggtg taggcggcgg gttgatggca gctgtgccaa
     7921 atcgttacgg cataggtgtc ttcagcccgg cactagatcc atttggtaac agcgcagctg
     7981 gtatccaatt gttgaaagat gtagtcaaag aactagacgc tgacatcttt gaataaccat
     8041 tcttgaaaga ggctggggta aaagtccaaa cggctcaaag ctacctacaa tgaggctgaa
     8101 attgtgagct tgtttgactt ttgtcccagt ttcttttttt attggttttt gaaagggctt
     8161 ttctaaataa aaaggaaaca aaaatacgat ttacagatat gattaaataa aatcataaga
     8221 aaaactggag gcgtgtatca ggaatgctgg aaaattacat cgagcgaaat atatttagaa
     8281 aagtgtatct gtgtgaacaa ttatttgaat tccaagaaat agacatcgaa caaacagcta
     8341 tttctctaag ggtgactaca ccaacgatcc ttcacgatct agaaagtcta gccgagtgtc
     8401 tagaatattg tataaaagaa caagtaagag agaagcataa gtataaactc gtattcaaac
     8461 atggtattgc tctttctgag ctcacacagt ttctgtacgg tcaatcttat tttttaaagt
     8521 ttttaagtta ttattttaat ggtatattca cgagtacaga actggctgat cttgaattta
     8581 tttctttgag taaagtatat acaatcaaaa aaatcgtatt ggattttttt aaagaaaatg
     8641 attatttgaa aaataaagaa atcattatcc cagaatttga ctcgcggaac atcttgctag
     8701 ctttagtacg ttatgtcaat tgggaaggct atgaaaataa aaatccgtat gtagaagaag
     8761 cttgtaacga gttgattgat tatgtagaga ctcatttttt taaaagaaga tattcggaag
     8821 atgaaaagtc ctttatcata cgagaaatag agatcgcaat cggaagaaag gcatttcccc
     8881 ttggactgaa agaagaagat aagaaaagcg cagaaacgaa gccgttattt caattagtct
     8941 gtcaaggatt ggaagagcaa aaaggaattc tagacttaca agaagaagat gtctattata
     9001 ttttttcttt gttcaacagt agaaattata ccaatgagaa catggagctg ttaagaaaag
     9061 atttcgaagt tgtttataaa aattttgttc tcaatgacct atcactgaat gaattgattt
     9121 tacagattat tagtatcgct gaaatcgacg atgcagaaaa ttttattttc aagcaatcct
     9181 ttttacaatt tgtccgtacg ttgtgggcag atggacaagt atttttaccg gaaaggatct
     9241 atcttctatc agaaaaagaa caacttctgt acggaaaaat tgtagaaata ttggaagagt
     9301 ggaaaaagaa aaatcaaatc caactaagat ggaatgaaaa tttcattcga aaattcgtca
     9361 aaagtctttc tttgatcaac agtgaacaag cggggagaca ggaaatagaa atttttgttg
     9421 tttcagaaag ttcagtcaaa caattttttt atcgaaccca atttcatttg tatgcggaag
     9481 aaggcatcgc tgaaataaat cctattttat ttcgttcatt agaagaattg cctgatgaat
     9541 gtctatgtgc gaaaaaaaga gtggttttgt gcgatgctgc tagctatcaa aatggattag
     9601 aaactgaaaa gacaaagatc tatccaatct cattaaaagg tgcaaacatc catttgtatc
     9661 atattgctca ggaacttcac ctattaaaaa aacatatttc ttgatgagac cttaattagg
     9721 agaccttcta caaagctttt taattgtctg tagaaggttt ttatttgagt cctgattcgt
     9781 gcgaagatct acttatataa caagctttct ctgaaatttt tttaaaatgc tattgactta
     9841 gagtcaactc taagtagtag aatagataaa gtgtaacaaa tctattttca ttaaaagaag
     9901 atagtacgga ggagagaatg tattatgagt acagtattac atgtattgga tgcaatcaat
     9961 agccccgcag atgtcaaaaa attatctgtt gaggaaatga aacagttggc agaagagatg
    10021 cgccaaaaaa tcttgcaaaa ggacagcgat gtaggtgggc acgttggtcc gaatctaggt
    10081 gtagtagaat taacgattgc gtttcattat gtatttgatt caccaaagga taaaatcgtt
    10141 tgggatgttt cccatcagtc ttatggtcat aagcttttaa ctggtcgaaa aaatggtttt
    10201 gaaaaaggac attaccgtga agtgagcgga tattccaacc aacatgaaag taaacatgac
    10261 tattttacag tggggcacac atctacatct atcagcttag caacaggttt agctaaagcg
    10321 agagatctga agaaagaatc aggaaatgtg gtagcattta tcggtgacgg ctccctaagt
    10381 ggtggcttag cttttgaagg attgaacaat ggtggtgttt tacaatccaa tctaatcgta
    10441 ttagtaaacg ataatgagat gtctattgat gagaatcatg gcagtttata taaaaactta
    10501 gctgagcttc gtcaaaccaa cggtaaaagc agcaataacc tatttaaagc aatgggattt
    10561 gattatcgtt acgtggataa tggaaacgat ctggaaacgg tgatccgttt atttgaagaa
    10621 gtaaaagata ttgatcatcc gattgtactt cacgtccata cggaaaaagg tcgcggttat
    10681 cagccggcaa tcgataataa aatgaaatat cattggcatg taccatttga tctgtccacc
    10741 ggggaggcaa agcaagtaac ttctggagaa acatttaata tgatcatcat ggattatttg
    10801 gataagaaaa tgcaaacaag tacagctcct attgccgcag tcaatgcagc gataccaggc
    10861 gcctttggac tgaaaacatt ccaagaaaaa catccagatc attattttga cgtggggatc
    10921 gctgaacagc actctatcac tttcgcttct gctttggctt ctcagggagt acgtccagtc
    10981 gtcttccaat caagtacttt cttgcaacgg gcatatgatc aattatcaca tgatttagcg
    11041 atcaacgaga atccagtgat tattttagtc ggtggcggaa cgatctcagc tagtgaccga
    11101 acacaccaag gaacatttga cattccttac ttatcaagta tcccgaacat caaatatctt
    11161 gctccagcaa caaaagagga tatgatctct atgctagatt gggcgttcga acaaacagcg
    11221 cagcctgtag caatccgaat tcctacacat ggtgtagaac atggtacagc tatcgcaaat
    11281 gattatagta ttccagccta tcaaacagta aataatggag gaaaagtagc aattttagct
    11341 ttaggcggtt ttcttgaatt aggaaaagaa acagtaaaag cttttagaaa aaaaggaatc
    11401 gaagcaacgt tgatcaatcc aggaacgatc acagaaaatg acgtagaaac attagataaa
    11461 ttaaaagaaa cacacgaatt agtcatcaca ttagaagatg gtagtttgtc aggaggattt
    11521 ggcgaaaata tcagtcgtta ttatggtgat tcttcaatga acgtattaaa ttttggtgca
    11581 gagaaagaat tcacggatag cgttcctgta gggcagcttt atgaacgtta tcatttaaca
    11641 ccaaaacaaa tcgttgaaga tagtctgcgt gtcttgaata ttcaataatt cattatgctc
    11701 accatccaga taattgttta tactagtatc tggatggttt tttcattata aaaagcaaag
    11761 gggtggatgt tatgtatcag caagtagaag aatatgtcaa aaagttccaa gaaaaatatc
    11821 ctagttatca atcagaaaaa ttaggtttct tttcatttgg ggatacagaa aaaatggcag
    11881 acgagttgag cgaactagtt gtaaaaaaga taaagactgg tacatcaagt ggttacgatc
    11941 tgtataaaca agagaatgag cctttaccaa aagttggtca attagacgtg gtcctgaatg
    12001 gaaaaaatga accagtttgt atcatcaaaa atataaatgt gaccatcttg ccttttagtg
    12061 aagtcacgaa agagcatgct tggaaagaag gagaagggga tcggacatta gcttattggc
    12121 ggaaagtcca tgctgagttt tttaaacatg cttataaaga agcgttgaac atagaattta
    12181 cagaagagaa gctggtcgta tacgaagaat ttgaagtaat tttcttttag taagaaaggt
    12241 ctgctgaaag aaatagctca ttattgtctc ctgaatagaa aagggttaca tttttatatg
    12301 aagctgtatt tttatataac attactggag gagatattgt ggatacactg gaacaaatca
    12361 aaggcggagt cgtcgtttct tgtcaagctt tggaaaacga gccgctacac agttctttta
    12421 ttatggggag aatggctttg gctgcaaaag aaggcggagc agttggtatt cgagcaaata
    12481 cgacagaaga tatcaatgaa atcaagcgaa tcgttgattt accggtaata gggattatca
    12541 aaagagatta cgatgggtca gaagtgttta ttactgcgac agaaaaagaa gtggaagaac
    12601 ttttggagac gcaagcagaa atcattgcac tggatgcaac ttctcgtaaa cgtccgacag
    12661 aaaaaacaac tgctgaactt gtccaaatga ttcacgaaca cggtcggtta gctatggcag
    12721 atatttcgac ttttgaggaa gcgcttcaag cgcaagaaga tggttttgat cttttgtcta
    12781 cgactttagc aggctataca gaatattcac gaaagacgca gacacctgat tttgatttat
    12841 tgaatcaaat catagaacaa gtcgatatcc cagtaattat ggaaggacat actgactctc
    12901 ctgaacaagt agagaaagca tatgaattag gtgctttttc agtcgtagtt ggatctatta
    12961 tcacccgtcc gcaattgatc actaaaagat atgtcgaagc cgcaagtaaa gcaaataact
    13021 aaaaaacacg cacggcacag attcttgaca aaaaagaaaa attgatagat agtttaaagc
    13081 agattctaag aagatgaaaa aagcattact tttcattttg tcttcaagat ctgcttttta
    13141 acgaatttta tagaaagaag gcgaaaccat gtttgaacgg agtccttcag aattgacaga
    13201 acgtgaaaat tacaagttgc tgattggttc agtcatccct agaccagtag cagtagtaac
    13261 gactaaatca gcaaaagata tagtgaatat cgcacccttc agttacttca atatcgtcag
    13321 ttcgaatcga cccatcttat ccttggcgat ccagagaaaa caagatgcag taaaagacac
    13381 agcaaagaat attcttgaga caagagaagc tgttatccat atccttgatg aggacaacgt
    13441 agaagaagta aataaaacag cagccaatct gcctaatgag gaaagcgaac tgtacctgac
    13501 tgacttgact ttaactgatt cagtaatcat ttcagtacta gcattgacag aagcaaaagt
    13561 tcgttttgaa acatccttat atgaacatgt cgagatacgg aataaaaata aagtaacagc
    13621 tgatttattt ttgctagaag tacagaaata tcatttaaat gaaacagtct atgatgataa
    13681 gacaggaagg atcgatccaa gaaaattgaa tgctgtaagc cgtttggcag gaaatgatta
    13741 tgcagcaata ggagaaattt tcacgattgc acgtcccgaa taaaaaggag cactagttat
    13801 gttaaaaata agctataaac catcgacaga ttcaaaagaa atgaaaaaag aatatgaaac
    13861 agtgaatgat tttctgcaag ggcagtactt agaagttccg ccattgcaag accattttgt
    13921 cgttactact gttactttag atggtaagga aatagaaatg ccagatcaaa cgattagcgg
    13981 attgtttaat tactttaaca aataaatatt tattccgaaa agtgaaaaaa catataaaaa
    14041 aacacataaa actaaaaaat tatcaaagaa tcctggtgca actgaaagaa tttgcaacgg
    14101 gattctttca acttatttgt tatttttttc taaaaaatat taaagattgt taaatttcac
    14161 tatttcattt caaattccat tgcctaatca ttaggaaaag tctatcatga actcgtaaat
    14221 agaatatagt aataaccttg ttaggtgagg ctcctataca aacataggct gttgcccaga
    14281 aacgtcgaaa gtcgccaatg ggtaaaacag aaactgttgg attaaggctt tttccaatag
    14341 ctaagaatgt tttctttacg ttgtatagtg ctaaaactcg gacgatgaga tgatgctagg
    14401 tcagaattct tgttcgaaaa ggtttctgac cttttcttta tatttttgtg actgacttat
    14461 ttgatgagac aaactcgctc ctcacctgac aagtggattg ctgtgtgagg aggagatgcg
    14521 gtgaaaaaag atcgattggt ggattttggg tccttatttg actttgtcca tgatcggtct
    14581 tttagaagta tacagtgcca gttcttatcg tttattgcaa gccgatgaaa atacaaaaag
    14641 tctattattg cgtcaactaa ttttcatatt tttgagttgg agcgtgatct tcttagctcg
    14701 ttcagtcaaa ctacactatt tacttcaccc taaaatagca ggatacggtt tagccttatc
    14761 gattttcttt ttagtattag taagaatagg gatattcggt gtcactgtca acggcgcaca
    14821 acgttggatc tctctgtttg gcattcaatt ccagccttct gaactggcaa atcttttttt
    14881 gattttttat ttaagctggt tttttcgtga cggaaatagt agcccaaaag atctaaaaaa
    14941 accattcctg attacagtag gtataacttt tctgatttta tttcagccaa agattgctgg
    15001 agcattgatg atcctttcga ttgcgtgggt catattttgg gcagcggcgg ttccatttaa
    15061 aaaagggatc tatctaatcg ttactttttc tgcattgctg attggagcag caggcggggt
    15121 attatattta ggaaataaag gttggcttcc acagatgttt aatcatgctt atgaaaggat
    15181 cgcgacttta agagattctt ttatcgatag tcatggtgcg ggctatcaaa tgacccattc
    15241 gttttatgca ctatataatg gtggcatatt tgggcgggga ttaggaaata gtatcacaaa
    15301 aaaagggtat cttccagaga cagaaacaga ttttattttt tcgattatta cagaagagct
    15361 tgggctaatc ggtgcattgt gtgtgctttt cttattgttc tcattatgca tgagaatctt
    15421 ttgtttaagc agtcgctgta aaaatcagca agctggattg tttcttttag gattcggaac
    15481 tttgctattc gttcagacga taatgaacgt gggaagtatc gcggggctga tgccaatgac
    15541 tggtgtgcca ttgccatttg ttagctacgg aggaacaagt tatctgatcc tttctttagg
    15601 aatcggaatc acgttgaata tttcttcaaa gatacaggcc gaagagttgg cgctctatcg
    15661 acctgaaaaa caataaagag aaactgagaa actctcagtt tgaattatca taaagtacaa
    15721 tcttttattt ttcaagcgag aggttctttt agaaagagga attcgcaatg aaagtatctc
    15781 aaaaaatcat attatctatt ttgttactgc ttacattagt agttggctat ttttcgattg
    15841 agtttatcca tggattttcg tctgcaaaac agagttcaac tgtgaaaaag gtagagccga
    15901 aaagtatccc taccacacta aatgtggctt tgattggttc agatgctcgt tcgaaagacg
    15961 aaaacggtcg ctcggattca cttctggttg cacaatacaa ccagaaaaca caacaagcaa
    16021 aactgatctc tatcatgaga gattcatatg tcgatattcc aggttacgga atgaataaga
    16081 taaatgcagc ttactcttat gggggagttg atttattaaa tcaaacatta aaggaaaatt
    16141 tcaaatttga agctccctat tatgcaagta tcacatttca agattttatc gattgcgtta
    16201 atgaactgtt tcctggtgga gtcaagatcg atgcagaaaa atctctagat ttagatggcg
    16261 tatatataaa caaaggagag caagtgatgg atggtaatac tttactccag tatgctcgat
    16321 ttcgggaaga caaagaagga gacttcggga ggatcagaag gcagcagcaa gtgatcaaag
    16381 ctatctctca gcagctgaaa gacgtaactt caatattcaa gctacccaaa gcggttggga
    16441 aattacttgg tagtatccaa acgaaccttc cagaaagtgt gcttctggat tgtggtatgg
    16501 atttcctcaa agacatatgt tagaataaac aggtataaat agtgaaataa aggaatgaca
    16561 agcaagagaa ggaggaaaaa atgaaaagaa gtgacaagca cggcaaaaat cgaacaggcg
    16621 cttatattgc cggcgcagtg atcttaatag cagctgcggg tggcggttat ttttactacc
    16681 agcactatca agaaacccaa gcagtagaag ctggagaaaa gacagttgag caatttgtcc
    16741 aagctttgaa caagggagat tataacaaag ctgcagaaat gacatcaaaa aaggcggcaa
    16801 ataaaagtgc attatctgaa aaagagatct tagataaata ccagaatata tacggtgctg
    16861 ctgatgtcaa aggacttcag atatcgaatc taaaagtaga taaaaaagac gattctactt
    16921 acagtttttc atataaagca aagatgaata cctcattagg tgaattgaaa gatctttctt
    16981 ataaaggaac attagacaga aatgatggtc aaaccacgat caactggcaa cccaacttgg
    17041 tttttccaga aatggaagga aatgacaaag taagcctgac cacacaagaa gcagcaagag
    17101 ggaatattat agatcgaaat ggtgaaccat tagcaacaac aggcaaacta aaacaattag
    17161 gagtcgttcc aagtaaactt ggagatggag gcgaaaaaac agccaatatc aaagcgattg
    17221 cttcctcctt cgacttaaca gaagatgcta tcaatcaggc gatttcacaa agctgggtac
    17281 aacccgatta ctttgtccca ttgaaaatca ttgatggagc aacgccagaa cttccagctg
    17341 gagctaccat tcaagaagta gacggcagat attatccttt gggtgaagca gctgctcagc
    17401 tgattggtta cgtgggagat atcacagcag aagatattga taagaatcca gaattaagca
    17461 gtaatggaaa aatcggtcgc tcaggtttgg agatggcttt tgataaggat cttcgtggga
    17521 caacaggtgg gaaattaagc attacagatg cagacggtgt agagaaaaaa gttctgatcg
    17581 aacatgaagt tcaaaatggc aaagatatca aattgacaat cgatgcgaag gcacaaaaaa
    17641 cagcttttga cagtctagga ggaaaagctg gatcaactgt tgcgacaacg ccaaaaaccg
    17701 gtgatcttct tgcgcttgct agctctccaa gctatgatcc aaacaaaatg acaaacggga
    17761 tctcacaaga agattacaaa gcttatgaag aaaatcctga acatccattc atcagccgat
    17821 ttgcgacagg ttatgctcct ggatctacgt ttaaaatgat tacagcagca atcggtctcg
    17881 acaacggcac tatcgatcca aatgaagtgt tgacgatcaa cgggcttaaa tggcaaaaag
    17941 acagttcttg gggatcttat caagcaacgc gtgtcagtag tgatgtatca caagtagact
    18001 taaaaactgc tttgatctat tccgataata tatatgcggc acaagaaacg ttgaaaatgg
    18061 gtgagaaaaa atttcgtaca ggcttagata aattcatttt tggtgaagac cttgatttgc
    18121 caattagtat gaatccagca caaatttcta atgaagatag ctttaactca gatatcttgc
    18181 tagctgatac tggatatgga cagggcgaac ttctaattac tcctatccag caagcagcaa
    18241 tgtattctgt ttttaccaac aatggcacac ttgtctatcc taaattgatt gcagataaag
    18301 agacaaaaga taagaagaat gtcatcagcg aaacagcagt acaaacgatc gtgccagatc
    18361 tgagagaagt tgtgcaagat gtaaatggta cagcacattc tctttctgct ttagggattc
    18421 cgttggcagc gaaaactggt acagcggaaa tcaaagaaaa acaggatgta aaggggcaag
    18481 agaacagttt cttgtttgct ttcaatcttg ataaccaagg ttatatgatg gtcagcatgt
    18541 tggaaaataa agaagatgat gattcagcaa ccaaacgagc atcggaacta ttgcaatacc
    18601 tcaaccaaaa ttatcaataa aaaattgctt tttaagtaca gtaaaaggac tgtggcgtta
    18661 tcgtcacagt ccttttttca aataaacggt tttcacgatg tctctttcac aacttctttt
    18721 tgatttactc acacttcata aacttttttt gtaaaatatt tgagaacaag tggagaaata
    18781 agtgtactta ataaaatcac gataactaaa ggtgaatagt actgtggttc gatcaattga
    18841 ctttgctgtc caatttgtaa aatgatcaaa gccatttctc cacgcgaaat cataccagca
    18901 ccgaccatca atgaactatg ttgagaaaaa ccggcaatct gagcaccaaa ataacctcca
    18961 agcaatttgg tcaaaatcgc tacaagcgtc aatatagaaa taaagagtag ttgttcacta
    19021 aagtcagaaa aatcaacttc taatcctaca ctgacaaaaa atactggtat aaatactgca
    19081 tatcctaaag cctctacatt gtggtatact tcttctttaa cctttgtctg actgacagct
    19141 atcccagcaa aaaaagcacc aatgacagag ctaagcccaa caagatccgc tacgtaagac
    19201 attcctaaac aaataactag cgatacgatg ataatagaag aatttgcgta cactttttct
    19261 gcaaatgaaa gtaggaaagg cgcaatccat ttgactaata agaaaataaa aacaaaataa
    19321 attagctgtt caatcaaaac aagcggtaaa gaagcattgc tagaagcttc ccccgttaga
    19381 aaagataggc tgaaacttag aactaaaacg actaaaatgt catctaccac agaagcaccc
    19441 aaaattgttg atccttcttt tgtattcact acgtttagtt cttttaatac ttctactgaa
    19501 atactgacag aagttgccgc aagaataatc ccaaagaaaa tcgcttcatt tcccgaaact
    19561 tggaatgctt tgcctgtcaa ccagccaaaa acaaccggga agatgatccc aagcaaagca
    19621 acgaacatac caggtcggaa atattttttc aaaagagata aatcactttc cagaccagct
    19681 aaaaacatta ataagatcac accaatctcc gaaaagtcat ggactagaat atcaggtttg
    19741 acccaattca atccagcttg gcctagcaaa acaccaacaa gtaattgtcc aatcactgct
    19801 gggataccta atctccgcga gatatgtgag ccaatcgttg ttgaaatcaa tatcaggcat
    19861 aaaataccaa taaattccat tctcatcatt cctttgtata aaatatagcc cgaacttagc
    19921 ataacataca ttattgaaaa aataggaaaa tatactcaca aaaaaaccaa caatccaaaa
    19981 gatttttttg gattgttggt ttttatacgg tctattttaa taactaaaaa tgattaacgt
    20041 atagtcattt ttcttattct gatgaaattg ataatctcct ggatgcccat gaaaatcaaa
    20101 ataaagccga atatttggaa tagaagcatc aaacttttga atggattgaa caataagacg
    20161 aatccgccaa tcaacaacag aatactacta taaacactcc aacttttagc ttttgtattc
    20221 atgctgaaga acaactggaa caagctgttg atgataacag ctaatccaag aagaatcggc
    20281 aggatagaaa cgattgcagg tgcaaaagca tagacgatca gagctaagat caggaaaaaa
    20341 agaccactga acagaccgat actccaagaa cctgttttcc ttttaaaacg gtaagcctca
    20401 aaaagattca acaacccaag caaaacaaga taggcggtga tcagataacc gataaaatga
    20461 aaaacagcag aagggtttag cacaatcgca atcccagaaa taatataaac agcagctctt
    20521 aataaggcgt atttttgaat attttgttta atcgtctcaa acatataaga aaacctcctt
    20581 tgacttattt taacgtgaac tagtcatttt accaaattaa atgttacaaa atgagcgaag
    20641 gagaagaata tctctggaaa aatcaacttc tgaggatctt ttccattttt ttgccttttg
    20701 ctagctcatc gatcatttta tctaaataac gaatttcttt catcaagtta tcctcgatgt
    20761 cttctactcg tacaccacaa acaacacctg taatcaagga acgtgaagga ttggggacag
    20821 gggcttccgc aaaaaattgt tcgaaagtca cttgtttttg tagttgatat tctagttctg
    20881 tttgggaata acccgtcagc caacaaatga tttcatctac ttcctctttt atacgatctt
    20941 ttctctccgc ttttgctaca tacataggat aaacgcttgc aaaacttgtc gtaaaaatac
    21001 gattttgcgc cataatcagt cacctttctt tttttcaaat tatttactac ttatagtata
    21061 taagaaaaag agaagaaaaa gcgattattc aaaaaaacaa aaaggattgt gacaaaattc
    21121 ttgtcacaat ccttttttcc gctctctgac tttttacaat cttcatagct caagtgcgca
    21181 tcagataatc gaatgctcct aaagctgcag tagctcctga acccattgaa acaatgattt
    21241 gtttgtatgg actatctgta caatcaccag ctgcgaaaac gccggggata ttcgttgcgc
    21301 catttttctc ggtcactatt tcatttcttg gattcatttc gatcgttcca tctagccaat
    21361 ccgtattagg gagtaagccg actagaatga atactcctga aacagctagt gtatgcatct
    21421 ccatcgtacg gcgatcgata taggagagag attctacctg attcgtaccg ctgatttctt
    21481 tcgtcgctgc atgtttgata acagtgacat tcgcaagaga gactagttta tcctgtaaga
    21541 tttgatctgc ttttaattct ggtaagaatt ctagaacata tacatgtttt gctaacccag
    21601 ccaaatcgat tgctgcttca acaccagaat taccaccacc gataacagca atctcttttc
    21661 ctttaaataa agggccgtca cagtgtgggc aataagcaat tcctttattt ttaaactctt
    21721 tttcgcctgg tacattgata gaacgccagt gcgcacctgt cgcgatgatg gcggtttttg
    21781 ctttaagtga tataccgtta gctaagccaa tattaataaa atgttgctct ttatttaagg
    21841 aaacggcctg ttgattcatc ataatgtcga tagggtaact acgcacatga ctttctactt
    21901 gctgcatcaa ttttggacct tctgtgtaag aggtaccaat catattttca atccctagtg
    21961 tttcaacgac ttgtccgccg aaagaatcag cgacaagtcc tgtacggatt cctttacgtg
    22021 ctgcatagat agctgcacta ctagctgctg gtccaccgcc aatcactaat acatcataag
    22081 gttctttttc aaacaactct tcttcaatca aagggccagt gattttttca acaatttgtt
    22141 cgatcgtcat gcgtccgcta ctaaattctt ctccatttaa aaacacagtt ggcactgcca
    22201 tgattttttt atcatcgatt tctttttgga acatcccacc ttcaatcatc gtatgagtaa
    22261 tatttggatt taatacagct aatatattca atgcctgtac aacgtcgggg caattatgac
    22321 aagtcaagct aacataactg tcaaaatgca atgtttgtgt tatttttttg attttaagac
    22381 tcaaagattc actgattttc ggtgcccgtc cactaatttg taacatagcc agtaaaaaag
    22441 aggaaaattc atgaccgagg gggataccag caaaaacgat tccactttct ttgccaactg
    22501 gattgataga aaaactaggt gtgcgcatcg aatctctttt ttcccaagag attttaggag
    22561 acatttctgc tatttccttc gtaaattcag ctagtttttg actattttca ctattatcca
    22621 aactaacaga aaaaacaata ggagattcaa tcagtgctaa atattgttcg agattttgtt
    22681 tagtttcttt gtctaacata tccaccacct aaatctttcc aacaagatca aaacttggtt
    22741 tcaatgtttc gccactttct ttccattttg ccggacaaac ttcacctggg tgggtacgta
    22801 catattgagc agcgcgaatc ttatcaatca gtgcactagc gtcacgaccg attccatctg
    22861 cattgatttc catagcttga acgattccgt ctggatcaat aataaatgtt cctcgctgag
    22921 ctaatccttg ctcttcatcc aaaacatcaa aaatacgaga aatctgatga gacggatcac
    22981 cgatcatcac gtattcgatt ttaccgatag catctgatgt atcatgccat gctttgtgag
    23041 taaagtgggt atcggtagag caagaaaaga cttcaacacc taaagatttc aatgtactgt
    23101 attgttcttg taagtcttct aattcagtag gacaaacaaa agtaaaatca gctggataga
    23161 agcaaagaat actccagttc cccataagat ctttttcact gacttctaaa aattctcctt
    23221 gatgataagc atgtgcatta aattcttcga tttttttccc aatcaatgac atagtaaaac
    23281 ctccaataat aattatttat ttataattat tgtaatttag ttggagttgc aaagtcaatg
    23341 gatattttgt tatttaataa agattactca aaaacgaagg ttaattataa aaatagaaga
    23401 ggtcgcaaga aaatgctact gctggaaata ctcgggataa cgttgagtaa cagtttcctg
    23461 ttctttcagc atcatgtgta agtgcaatcc cgtttgaaat cgagcttctt ctggtttttt
    23521 atcaactaaa gccttgagta aatcataatg ttgttccatg atcgtttccc atggaagccc
    23581 ctgaatggtc actcgtaacc agcggaaacg ttctaaatgg gtattatggt catctaacca
    23641 gttccaaact tcttttcttc ctgcaatttc gaaacaagct tcatgaaata aattatctgc
    23701 acggaaaaaa gcgcgttcgt catttatttt gactgctttt tcttgttctt ctaaaatcgt
    23761 ttctagcact attcgtgttt gtgtgtccaa tttggaacaa cattcaatca tgatttgctc
    23821 ttccaactgt tctcggacaa agcgagcatt ttctgcagaa acaagattaa ttttggaaac
    23881 atacgtacca ctttgaggta tcgtatacac gagttcttgc tgacgtagtt gaatcaatgc
    23941 ttcgcgaatg ggagtccggc cgatagcaag atgttcctcc agtgcctttt cagatatttt
    24001 ccttccaggt tctaagtcag catagataat ttgtttacga atcgcttgat aagcttgtga
    24061 ttgcaagctg cttttcatct ttattcctcc aaaatcattt ctatgaacgt agtataacag
    24121 aagaggtctg aaaaacaatc tggtatgtca cattaactag taaatttgta taaatagtaa
    24181 aattttggtt gacatagcga tttttattgc ataaaattca cttgaataca aaaactaata
    24241 tgttagttta ggaggggaaa aatgattgat ttaaacaact ggaggaactt tatccctgaa
    24301 ttatctcaaa aagaagtaga aatacctcat tttgaccgtg catcattaag agaaaatgga
    24361 caaaaaaatc ctgtctggat ccatttcggt ggcggaaatc tttatcgagg atttcatgca
    24421 gaaatcgctc aacgtttagc ggatctaggc gaattgtcca ctggtgtcat tgtttgtgaa
    24481 acattcgatg aagaaataat tgaaagggct taccaagatt ttgatgattt gattcttgaa
    24541 gtggtcatgc caagcagtgg agagttcgtc aaacgtatat tagctgcgac agctgacagt
    24601 ctgtattgtc atccagattg ttctgaacac tttgataaga tgattcgaca ctttgaaaat
    24661 cctagtttac aattagttac ttttgctatt actgaaaaag gttatcgatt gaaagataca
    24721 aataataaat atataggacc gattgcagtc gatataaaaa atggtccaaa gaagccatca
    24781 catacaatga gtattcttgc tgtgctactt tataaacgtt ttttatctgg agcttggccg
    24841 attgcacttg tctctacaga taatttttca caaaacggta gtcaattacg tacttctttg
    24901 ctaacgatcg caaaagaatg gcaaaaggaa ggttatgtac aaaatacatt tatccaatac
    24961 ttatcgaatg aagaaattgt tgcgtttcca tggacgatga ttgatcgtat caccccgaat
    25021 ccttccttgt cagttagcaa aatgttggca aaagagggct tttcaaatat gtcgattttc
    25081 caaaccgcaa aaggaaataa tattgctcca tttgtcaata cagaggacat ccattattta
    25141 atcatggaag atcattttcc caatggacgt ccgaaattcg aaaaagctgg ggtattcttg
    25201 actgatcgtg caacggtaga taaagcagat acgatgaagg tgacgacttg ccttaatcct
    25261 ttgcatacgt ctttagctat tatgggttgt ttactggggt ataggagcat agctgaggag
    25321 atgaaagatc cagatttatc gctgctagtt tctgaaatcg ggtacaatga aggcctccca
    25381 gttgtggagt ctccaggtat cattgatcca aagatctttc tggaagaagt aattactgtg
    25441 cgcttttcta atccaaatat tcctaatatg cctcagcgaa tcgccacgga tacctctcaa
    25501 aaggtggcga ttcgttatgg tgagacgatt aaaaaatata tggaaataga agttaaagac
    25561 ccgcagacac ttgtattcat accattgact ctcgctgctt ggcttcgata ccttattggc
    25621 attgatgatc aaggaaaacc attcatcccg agtccagacc cactattgac agaccttcag
    25681 cataagcttt ccgatattca actgggtacg caagatacat cacatattca tgagaaaatg
    25741 gctcctattt tgtcaaataa agaaattttt ggtgtagatc tttaccaagc agagcttggc
    25801 agaaaaatag aagaaatgtt catcaagatg gtagcaggac caaatgccgt acgaaaaaca
    25861 ttacaggatt acttacaaga acatggagga aatttatcat ggaaatgact tttagatggt
    25921 acggatcaca ggaagaaaag attaccttag atgaaatcag acagattcct gcggtcaaag
    25981 ggattgtagg cacattgatg gatataccag taggagaggt atggcctagg gagaggatca
    26041 aagaactaaa agaagaaatt gagtctcgtg ggttaacact aaaagtaatt gaaagcgtca
    26101 atgtgcatga tgccattaaa attggattac cttcccgtga gcagtatatc gaaaactata
    26161 agcagacaat ccgaaacttg gcagaatatg gaatagaagt catctgctat aattttatgc
    26221 ctgtatttga ctgggtgaaa tctgaccttg actaccgctt agaagacggc tcatccacgt
    26281 tagcttttat atctgatgat attccagatg atccaagaga aatcgtcgcg cgcatcgaac
    26341 attccagcaa tgaatttgga ttgccgggat gggaacctga gcgcttagct cctatcaaat
    26401 ccttgcttga agcctatgca tcagtagacg aagaaaaact tcgagaaaat tttgcctatt
    26461 ttctccaatc aatcatccca acatgtgaag aggtaggggt caaaatggct gtacatccag
    26521 atgatccgcc atactcgatt tttggattag ctcgaatcgt aaagaatcga gaagatttag
    26581 attggttgtg tcgcatagta gacagccctt ataacggaat cactctttgt acagggagta
    26641 ttgcagaaga tccagaaaat gatgtatatg ggattttagc agaattttgc caaagagatc
    26701 gaatcccatt tgcacatgtt cgaaatatta aatttttagc taatcgagat ttctatgagg
    26761 caccacataa atccgaatac ggttcattag acatgtaccg catactaaaa acaatgtatg
    26821 acaacggatt taacggatac atccgtcctg atcatggaag aatgatctgg aatgagacag
    26881 gaagaccagg atacggctta tatgatcgag cattaggtgc tgtttatcta aatggattat
    26941 gggaagcaat cgaaaaagga aacaaaagta aagataatta gtgaaaaaaa ctgtaatttt
    27001 attatgtaaa taagaaggat gttcaaattc ttgatacata aatccggagg ttgttaataa
    27061 gcattcggat ttatgtatct tatttggtct aggaagtaac ttgattttct tttttcgatt
    27121 gatccatgag tgcttcttct tttaattcaa agatatattc tgagatcctc ataatcactt
    27181 ttttccagtc ttctggtgtg atgattcctt ttaaaatata gatttttttg atatgagaag
    27241 gcaattgttt caaagggcag tcgctgataa cgatgttgca gttctccata aaatcatttt
    27301 gtattttgat cgattcattt gaaaaaattt gttctatctt aacgatcaga tctttactga
    27361 tataaaaatc acgcgtatag ttcacaccaa tcacaattcg cggctgtttt ttgtagcgat
    27421 catacaaatg tactaggtct tcaaccatcc attgcacatt ttccttttct aaaataactg
    27481 gtaacaagtg ttgattttgt tctctgaaat ccatataaaa ttgttcaatg gcggtttgga
    27541 cttctttcaa tacgctatgt tcttgataaa gcatgaaagt aggaaggggc gtatttggtt
    27601 gattgaagtt aagaaatcca ttataaagtt tatttcgtgc gaatgccaat gagtaaagaa
    27661 tcttttcttt ttcaggtata aacaaatcaa atacagtaca aagggactcc accatttctc
    27721 ttgaataaag aacatgagga acgtttgctt tttttaaaag tttgtattgc tccaagcttg
    27781 tttcttccga ttcgattttc gtgatagaaa gtcgagctaa aatattcact aagatgcgtt
    27841 gttcgcgaca aattggcaga ttaggtcgca aaatatctac agagagatca ataaaaaagt
    27901 caatatattc atactctgtg aattcttttt gtacgtcttt cacagaagtt gcaggaaaat
    27961 tctctttaca tatttttaat agaaaatata atttatctat cataccgcta tttagtcggt
    28021 caagtacttc ttttttaaaa aaagtattca tcataaaagc cactacttgc ggatcttcat
    28081 cataaaattc tgtagcagta cttgaaaaga tgttccaata aatatctaat gctgcatatt
    28141 gaatttgtac ctcttttcca gaaaaagact ttctgccatt attagaaaag gagatagaca
    28201 aaccgtacta tgctaaaaat gcattaatct ttttcatttt cgtatacaaa taagaggcac
    28261 tatagtttgt ttgctgactg aaactgataa tcgaatttgt ctgacagctg aacaaagaag
    28321 tcaagatctg ataactgact gattcgttgc agtatcttcc aaatagacga taatagcagt
    28381 aagccggatc aattccttca aaattcaact ttaaaaaagg tttttgctta tcaatatgca
    28441 gttctgttag attgagacgc tcaaagtctt gttctaattc tttgatgtat agtaaaaaat
    28501 ttgattcaga catatggaaa tgatctgcta aatattgtgt agtaataaag aaacgatgtt
    28561 cagataggaa aagaaaaata tctaattttg tattgctttt tttcttcaag ataaacgttt
    28621 taatcgcttc ttgagtttgc atgaaagagg ctccttttta cttaaattgt taaaacttga
    28681 aaatattact gtttttttca taaatttaga caaaaaaaca gtaaacatat aggtagaatc
    28741 tctactatct tatttcttgc atacaaaaaa atcaaggagt gagagagtta tgagatattc
    28801 aaccatctta atatcaacat ttgcagcctt aagcttatcg cttactataa atcagacagt
    28861 ccacacagag gagacagaaa cagccaaatg gacagaaacc aagtgggcca acgtcaatcg
    28921 aattaccttt caggatgaat tttcatcctt caattcagga gtcgcaaaat taagaaaagg
    28981 acaaacagcg accatcaaag caaaaattga ctattcggga gacttagcac ctatactaca
    29041 atcagctgtt acatttgaga atgttgatcc cactttgtcg attggctatg acgagaacac
    29101 tttgactatc caacacaagc aagcgatatt taccttcacc gttacattaa atcaggactt
    29161 gttaaaacca gcgcttttta ctgtcaaggt atcagatgga ctaccgaacg atcatcattt
    29221 aacgccgtat agtcagcggc agacaattga gcaagattca gccatcgact caggaggaga
    29281 tcttgttgaa gaaccaacag ataaaccaga aaatgaaaat aagccagaag taccgcctac
    29341 agaaaatcca gacggagaac agaaaccgga aatagaacct ggagaagaac ccgatacaga
    29401 aactcaacca gagccagaca ataaatcaaa acctgaaata acccctggag aaaaaccaga
    29461 tgtaactcca gaaccagata cggattctgg aaaccaaaca gtaccggaaa caaatccaga
    29521 cacagataat gaaactgaaa atccagaaaa accagaggta gatccagaag aaaaaccaga
    29581 tgtgactcca gaaccagata cggacgcaag agaccaagga ataccagaaa aaataaacaa
    29641 aaaaacaatt caagaggatg gaaaaaaaga gagtaaaaaa tcaaatctag caattctaaa
    29701 aataaatgaa gagcagctga ataaaaaatc acgaatattt gatagcgccc aatcagctga
    29761 aaccttaaaa agtagcaagg atacaacctt tgctgtcctg aaacgaaaaa taaacagcta
    29821 ccaaaatccg gagagtcaca aaacaaagtt atactgtgga gcggaatcat tctattaagt
    29881 atagcaacaa tgctttctgc aaaaagattt aaacaaaaca gatctctcta acatttgaat
    29941 aatgtctgtg aacgcatttt tcaaataaaa agaaatatcc agaaaatgac cccaaaatac
    30001 cagattgaaa tctgatattt ggggtcgttc atactttatc tttcttcata tttattttaa
    30061 gggatatctg tttcttttgg aagatggttc gggatctttt tcactttcaa tacatctaaa
    30121 aagaaggtaa agatcggaat gccgacaatc aagccccaaa cgccaaagaa ccgttcgcta
    30181 acaagtaaga ccacaaatgt ataaaaaatc ggcagttctg ttttgctgga catcaacttc
    30241 ggattcagca cataagactc aattagatgg acaatcaaaa tcgtaatcag gatataaaca
    30301 acatctcgga tgcctccttg ggaataagct atgaaagata gaggaataca agaaatgata
    30361 acacctgcta ctgggatcag acttaaaatg aaaatcatga tcgctaaact cagcagctga
    30421 tgaaagccaa atacagccaa ggcgattgta gtgagcaacg tatttaaaag agcgacaaca
    30481 aattgagctt ctaataccaa accaaatgta ttgacaaact tatcggcaaa atagtaaata
    30541 tcttgaaaga accaagaaaa ttcacttttt aaaaacaatc ttgagaatac aattgttttt
    30601 ttcttttcaa tcataaaaaa gaaacttaag atgaatgaca tagcaaacgc cactgccatt
    30661 tttcctatat cctgtaaata gccaagcgca atcgaagcgc cattttgaag ctgttcaaaa
    30721 aaatcgtttc gctcaatata agtatggata tattgtaaaa caggatctgc atcttttggc
    30781 tgatgctcgt aaaaattaat aacggactgg atcatgtcaa atgtttgatg aataagcatc
    30841 gggatatact ttgtgaatgc cagatagata aggtaaacga tcagtgtata aatcatgata
    30901 gctaaaacac ctgtagggat tcggacaaat cgctgaatga tttgaacaag atgaatcatc
    30961 aaataagtaa agataaatgt gagcaaaatg gttgtaatca tacttctaac taagaaaaga
    31021 acaaaaacga caaataataa aacagtgaga cggcgtattt tttcattcgc caaaaaacgc
    31081 tgataaacgg tcaaatcgac aacctccaaa taacaaaaaa taaacacgtt attcattata
    31141 tgatggaata tgaaaataag aaagagcaaa aagaaaaacc gctaaatgac gttatcttat
    31201 gagttggttt tcctcataaa atcgttcatc tagcagtttc agcccttttt ctggtcttga
    31261 tgaaaattat ctagcatttt tcgggtcaga tgttgcagtg tatccaattc ttcctgttca
    31321 agtggaagat aagacttgat cttttgtgga ataccttctg cttgtttttg caacgcagca
    31381 ccttgagcag tcagcgtaac atttacgatt ctttcatcac ccgtttcacg ctgacgatta
    31441 atataatttt tttctgctaa cttttttagt aagggagtca gcgtaccaga atccagacac
    31501 aaatcatttc ctagctcttt cactgaagtc tttttctttt cccataaaac taacatcaca
    31561 agatactggg tataggtaag attcagcggc ttgaggaggg gagtgtagtg tctaatgatt
    31621 tctttagagg cggcatataa aggaaaacat acttgattaa ctaaatatag atcttccatt
    31681 tttccacctc cgctgtttta ctatagtgta tcatagcgga tttacacaaa aaagtctatt
    31741 cactaactgc tttcagagta acttctatat ttccagctgt agcttttgaa tacggacaca
    31801 ctttatgcgc tgcctctaat aattcttgcg tttcttccag agaaagacct tcgatagatc
    31861 cttcaatctc tacacctaag acaaagctcg tttcactttc tgaataaagg gaaacttgtg
    31921 cactgacagt agaagcccca gagatatttt tttgctgttt gatcaattct aatgcactgt
    31981 taaaacatgc actgaaacca gctgcaaaca attgttcagg gttggttgca ttttcgacgc
    32041 gtttaccagg agctgcaata ttcaattcga atgtacgatc gggattatgc acttctccag
    32101 agcgtccgcc tgtgttgatc attttcgtag aatacatttt tttcataaaa ttaccacctt
    32161 taattatatt ttatacaatt gaattttaca caatataatt gtggaaagca aagaaatgga
    32221 ttggcataag ataaataaga gaaagcacat gaaacagacg cgctttctcg aatagcaaac
    32281 atatacaaaa aatatcagag acaaaaaata atctcatcat aaacattaca tatatttctc
    32341 aaaaagttgt tttctttctt caaaaattac ttcacgatgg acctgatgaa gcatccataa
    32401 ataaccaaaa ggatctctga acattgagtt agtcacacca aagtccatca tttctgtcat
    32461 ttcttgaatc tctacacagc catttcgtaa tgctttatta tgtgtgtcct cgatatcaga
    32521 gacgatgata ttgaaccaga tcgtatttgg atgttcagca gtaggagcag tcaaaccaag
    32581 agacgtgttt tcatccagca tatggaatct cgtaccatac aaggtaaaaa tgacttcatt
    32641 attgcctcta gggtaatctg ttgcctcaat cagttgtatt tcatcaaaga ttgtttgata
    32701 cagttctagt gctttcaaac tatcatttaa tacaaaatcg atttccgttc ctttatgcat
    32761 aaagatctcc tctccttttc agtcgaacag ttactagatt cttttattat accagctaaa
    32821 gaaaacgcat tcaaaaaaat agcggattta cttaccaact cattcgattg gatgataagt
    32881 aaatccgcgt cccattactt ctgctacgct gctgacaatg acaaaggcac ttggatcgat
    32941 ttcttctatc aatggtttca ttcgggaaaa ttcatgatca gtcacaacga tcaataaaac
    33001 ctcttttgac tttcgattat acccgccttt tgcctctagc agggtaaggc cacgtccaat
    33061 ctcagcactt aagcgaaggc ggatgtcttc caaataatgt tcactcatga ccataacaga
    33121 tttctttcga ttgatacctg tttcgatata agtcatgaca attgaagtga ttgcaataga
    33181 taaaatagca taaagaaact cttctattcc aaaaataaac agattaagag aaacaacgaa
    33241 tgcgtctgta aaaaataagc caatcgaagt attcaagtgg aaatattttt taaaaatgag
    33301 gggagggatc gtcgtaccgc cgctagaaga gttgttttta tataaaatcg caactcctaa
    33361 agcaaaaatc gcacttccaa aaatgacaga caacaaccga tctgaagtaa tcattgtttc
    33421 tggaactatt gcaatgatta aaggaaaaac caagcttccg aaaagtactt taaaaaaggg
    33481 ttttttacct aaaaatagaa gagctagtac caacataacg atattcaaca ctaaaataac
    33541 tatagctcgg tcaaagccta aagcagcttc cagtaatatt cctagaccgc tgacacctcc
    33601 agcagcaata tgatgaggcc ctaaaaacat attgatagcg actcctaata tgaagagggc
    33661 aaaaataatc ttaatgtatg tccatataaa ttgtctcgtt tctttcattt attcacttcc
    33721 ttaattcatt aataatactc ttatcttatt ataacttact tttcacctta agttaacttt
    33781 aattaataaa aaaattaggc tagtctaaat tattatgttt cattcttcta gaatatactt
    33841 ctacgccaaa aactgttaaa aaacttttac tttagaaaat tggagaaatt gatcgcttaa
    33901 gataaattca ggacactaaa tagtacagga agtagactaa aacttttaaa agttttaagt
    33961 ctatctattt ggcaagttta aaaaataaaa attcggtatc aaaaaactgt taccgataac
    34021 atttttgaaa gcgttgtctt ttgcttctct atcacttata gtgatgacgt catattaaaa
    34081 cacaaggagg aaatcaatgg atacagctgc aatttatcat cgaccggaaa gtgaatatgc
    34141 atttctatat acgaatacaa gatttcgtat tcggcttcgt actaggaaaa atgatatcaa
    34201 aaaagtatat gtgctatgtg gagatccata taccataaca actgaaaaat ggtatcaaaa
    34261 acaacgacca atgaaaaaat gtctaagtac caatgttcat gattattggg aaattgaagt
    34321 gacgagcgaa acaagtcgct tacagtatgc gttccatgtt gttggggaag ataacacgga
    34381 ctgtttttac ggtgatcaag gaatttttcc ttatcgagaa aatgtgatta ctgaaccaaa
    34441 tttttatttt cgaatacctt attttcatca aatcgatcga ttcactattc cagaatgggt
    34501 aaaagaaaca gtatggtatc aaatttttcc cgaaagattt gcaaacgggg acaaagcaaa
    34561 tgaccccgaa cacactttac cttggggaag taaagatcca gaccgagaag actttttcgg
    34621 gggagatttg caaggtgtga tggatcattt ggattacttg acggaccttg gcatcaatgg
    34681 catctatttt tgtcccatat ttaaagccac atccaatcat aaatacgata cgattgatta
    34741 ttatgaggtg gatcccgctt ttggcgataa aaaactttta aaaaacttga ttgatgaagc
    34801 acataaaaga gggatacgta tcatgttcga tgctgtattc aatcatatgg gtgtccattc
    34861 accacagtgg caagacgttc tcaaaaacgg agaaaaatcg atctataaag actggttcca
    34921 catccgtttt ttcccagtag attcttatca gatgactgac ttaccagaaa ctgctgaaaa
    34981 tatcccatat gatacttttg cttttactcc attcatgccg aaactaaata cagcaaatcc
    35041 agaagtgcaa aaatatttgc tggatatcgc aacttattgg ataaaagaat tcgatattga
    35101 tggatggcgt cttgatgttg ctaatgaagt cgatcatcat ttttggaaaa aattcagaga
    35161 agcagttaca gaaatcaaac cagacatcta tatccttgga gagatttggc attcctctca
    35221 agcttggctg caaggggatg aattccatgc agtgatgaat tatgcattta cagattcgat
    35281 taaagattat tttgcaaaaa agaaaatcac agcttcacaa atggtcagtg gaatgaatca
    35341 tcagcaaatg ctttacagag atcaggtgaa cgaaggaaca ttcaacttat tagattctca
    35401 tgatactgcc aggatcttga ccctttgcca aggtaacaaa gaattgatga agtctgtgat
    35461 ggcctttatg tttcttcaaa aagggtctcc ttgtatctat tacgggacag aaatagggat
    35521 gactggtgaa gatgacccgg attgccgcaa atgtatgata tgggagaagg aggaacaaga
    35581 tctagagcta ttcggtttta taaaagaact tgtttctcta aggaaacagt tgtctaagat
    35641 tatttctgaa ggttctactc agtggctaat agtaaatgat cgtgaagata agctctattt
    35701 cacacggaaa ctagagggac agatcatcta tgtttacttt aatcagtcaa aagaaccttg
    35761 ggtagtagaa caggaaaatg aggtgattct tagtcaaaat tgtcagttgt tagaagacgg
    35821 gaaagcagag atacgacaat acggattttt gatcatgtgt tgaaaactgt taccggtaac
    35881 aacaagaaag cgtttgcaaa tgaagatttg tgcccattat aataagggtg taacagcacg
    35941 ttgaatacaa aggaggaaat gaagatgaaa aaaagaagtg tgaaacagtt gggtttaggg
    36001 ttaatctctt taggaatctt aggcggatta gcggcttgtg gcaatggtaa ctcttcagca
    36061 gaaggggaag ccgatgaagg aaaaacgtta acagtttccg ttgattctgg atataaagat
    36121 tatataaacg aaataaaaaa cactttcgaa aaagaaaacg atgtgaaaat caagctagta
    36181 gaaaaggaca tgttcgatca gttggaatca ttagcgttag atgggcctgc tggaaaggga
    36241 cctgacgtta tgatggcagc atatgaccgg atcggtgcgt taggacagca aggacatctg
    36301 gcggaagtaa aactgggaaa tgaagcagct tacgatgaaa cagacaaagc ccaagtgact
    36361 tatgatggaa aaatctacgg agaacctgca gtaatcgaga cccttgtttt gtactataac
    36421 aaagatttag tcgatacagc accatcaacg tttaaagatt tggagaatct atccaaagac
    36481 agtcgttttg cttttgagtc agaagcagga aaaaatacag gatttttagc taaatggact
    36541 gacttttatt attcctatgg tttaattgca ggttacggtg gctatgtatt tggtgatgat
    36601 ggcacaaatc cttcagatat cggtttgaat aatgcaggtg ctgtagaagg aatctcttat
    36661 gcaacagatt ggttcaaaaa tgtttggcca aagggaatgc aagacataaa aagtgcagga
    36721 gacttcgcca gccagcaatt catgtccaat aaaactgcag caatcattga cggtccatgg
    36781 caagcgcaaa catacaaaga aaacaatatc aattatggtg tagccaaaat tcctactcta
    36841 aataatggac agccttatca accgtttggc ggaggaaaag gatgggtagt cagcaattat
    36901 gctaaaaaca aagacctctc tcaaaaatgg cttgattatg taacgaatca ggaaaatcaa
    36961 gaaaaattct ttgaaatggt aaatgaaatc cctgcaaacc aacaagctag ggaaacagca
    37021 aaggctaaaa acgatgaatt aacaacagct gtgattgaac aatatgaaac agctcaagcg
    37081 atgccaaaca tcccagaaat gggcgaagtt tggacaggtg cagaaaactt gatgttcgat
    37141 gcagcatcag gttcaaaaac accaaaagaa tccgcagatg aagcagtaaa aacaatcagt
    37201 gaagccatag aacaaaaata ctgattaaaa tgataaccgt ccatttttat aaaaaatgga
    37261 acaggtgctt cagataaaag tggcaagagc gagatggaaa attatcttgc tcttgttgcc
    37321 attatcttaa aagcagtttg cggtttagca agtttagagg gggcattcag atgcagcaag
    37381 ataaaacaag caatatcaac gaaaaaaaaa tcacgctttg ttcacttatt ccagggatgg
    37441 ggcagtttgt aaatggccaa gcttttaaag gtatcgtttt tttcgccata ttcgtcctat
    37501 tcattggtca gatgcttctg ggcggatttc atgcattggt aggattggtt actttgggaa
    37561 gtataccaat ggaagatcat tcattattta ttttgattga aggcactttg caattcatca
    37621 ttacaatcat tttcttgatt ttttacggtt tgaatctttt agacgcttat cgagtagcga
    37681 agatgcgtca agagggcaaa aaaatcaatc ggacgatgaa ggaagtcata cataatatag
    37741 gtgatgaagg ttttccttat ctattgacac ttcccgctta tcttctcatg atttttacga
    37801 tcattttccc agtcctagtt acattgttca ctgcatttac taattatgac ttcaatcata
    37861 ttccaccagc cagtttaatt gattgggtag gggctaaaaa cttttttagt atcttctttt
    37921 taagctcata tcgtaatact ttttttgcag tcttttcgtg gaccgtgatt tggactttat
    37981 gtgcaacaac tttgcaaatc acccttggta ttctcacagc ggttgttgct caccagaaat
    38041 ttattaaatt caaacgtatt ttcggtatca ttttcttatt accatgggcg gtcccagctt
    38101 ttattactat catgagtttt tcgaatatat tcaacgatag cgtaggtgcc atcaatactc
    38161 aagtgattcc tttcataaac cagcttcctt ttgtagatat cagtgcaatc tcttggaaaa
    38221 cggacccgaa ctggacgaaa gtagcaatta tcttagtgca gggctggtta ggatttccct
    38281 atatctatgt gatggttaca ggaatcttac aatcgatttc agaagactta tatgaagcag
    38341 caaaaatcga cggagcaaat gcatggcagc gcttttcaaa catcacgctt cctgctgtct
    38401 tcctagtggc agcgccaaca tttattacac agtatacggg gaatttcaat aatttctcca
    38461 tgatctatct gtttaacaat ggcggtcccg gaagcgtagg cggaggagct ggttctactg
    38521 atattcttat ttcctggatc tataaactta cgactggaac ttctccgcag tattctatgg
    38581 cttcagcaat cacgttgatt atctccgtca tcgttatctg tgtgtcattg ctggtattta
    38641 aaaagacaaa tgcgttcaat atgggggatt aacaatgaaa aaatttcatt cgagtgctcg
    38701 attccaaaaa cgactttctc acttttttac ttactttttt cttttaatac tgtcagtcat
    38761 tatcatctat ccgcttttga tcacagcaag tactgccttc aagtcaggaa atattgcagc
    38821 ttttagttta gatctttcaa gtgactggac tttaggaaac tttactcggc tattcagtga
    38881 aacactttac gggacttggt ataaaaatac attgatcatt tctgtcctaa caatgatcgt
    38941 acaagtgact gtagtaactt tggcgggcta tacttatagt cgctatcgtt ttatcggccg
    39001 caaatcaagt ttgatgttct ttttagtgat tcagatggta ccgactatgg cagcattgac
    39061 tgccttttat gtcatggctt tcttattaga agcgctcgat aagtactggt tcttgacaat
    39121 gatctatata ggcggtggaa ttccaatgaa tacttggctg atgaaaggat actttgacac
    39181 tgtaccaata gatttagatg aatctgctaa acttgatggg gcagggcatt tcaggatttt
    39241 tttccagatt gtattaccgc ttgtcaaacc gatgctcgcc gttcaagcat tatgggcttt
    39301 tatgggacct tttggggatt tcatgttagc taagttcctg cttcgaacac ctgaaaatca
    39361 gactgttgct gtcggcttac aaacatttat ttctgatgtg acaaaccaaa gagtctctct
    39421 ttttgctgct ggggcaattt tgattgcact accgatttgt ctattattct tctttttaca
    39481 aaagaacttt gttaccggcc tgacatctgg cggaacaaaa ggataggagc agagtatcat
    39541 gcaaacattt ccgttaaact atttttccgc attacgtacg ccaactcaag tgtttgcggg
    39601 acgcaaacta ttatcatggc ctaaattttt cttgatattt gtatttttag tttccttgat
    39661 ggtcatgcct gtgactcttt tttatgccaa tcagatccaa gcgattccat tagaacaatt
    39721 tctatccgtt cattcgctga tcgatgaaca aggaacccaa aaattttctg aacttgaatt
    39781 atctgaaact gggttacaag catcgcaaca aacgatagct gtgacccctg aaattttggt
    39841 aggtgtgtct ttatctgaga aacaacaatc cgatcatgga acttttattg attttgagaa
    39901 ggaacaatgg gtgatccaac aaaaggataa atctggtata cgccgctata cgatgaatta
    39961 tagcccatct ttccaaccag attctgtacg tacgcccgaa gatttccaac gatttttaga
    40021 aagagaattt tatgccagta atcgtccaac aatcatttta tcttatagtt taagtcttgg
    40081 tcttgtccta tttgtcatga cgagtctcat tttattcggg gcttcctttt tcttgtggat
    40141 gacaagaaaa agccaacttt catccattca tacattcaaa gagagtgcaa atttgatgct
    40201 gaatgttatg ggaataggtt ccatggcagc agcagttgta gggtttatcc actttgactt
    40261 tatcttgatg ctaagtgtcc aaacaacgat aactgtcttg ctgcttttat gggtttttgt
    40321 caaaacaaga ttcaaagata agcgtgtcga atgacaagtt taagcgattt atgcgacaat
    40381 gaatgtgaaa aaaatgaaaa ataaagtagt ttttgaaaaa tttctgaaaa aattaaactt
    40441 cttagttgat tcttttcttt tttagtatta acgtaatagg gtagtttttt taaggaggaa
    40501 aatcatgtta gagaaaactt tatacaatca attattttca cgttctttct cgctccctgt
    40561 cgaagtaaat tattgggacg gtacaacgaa acaatacgga gatacggaca ctcctcccca
    40621 aatcaaaatc aatatccatg aagaaatccc aatgaaagag attacaagta acgcatcttt
    40681 ggcactagga gaagcctata tggatcatcg aatcgagatc gagggaagta tccaagcatt
    40741 gatcaacgat gcttatcagc aagcaaacag ctttttacga ggaaaagatt atctaaaatg
    40801 gctgccaaag aaagaaaaac atacgaaaca aaaaaacaaa gaagatatcc atgcgcatta
    40861 tgacttaggt aacgactttt ataaactttg gctagatcca actttgactt attcttgtgc
    40921 ttatttcaat acacctgaag atacgttgga acaagcacaa atcaataaag tccatcatat
    40981 cttagacaaa ctctttatca aagaaggcga aacgttgctt gatatcggtt gtggctgggg
    41041 aacattgatg ttcactgcta ccaaagagta taatgtcaag gcaactggga tcacactaag
    41101 tgaagaacaa tacgatttga ttaccaaaag aataaaagaa gaacatttag aagataaatg
    41161 ccgtgtatta ttaatggatt accgcgaact aaaaggcgaa acgtttgatc atatcacaag
    41221 tgtaggtatg ttcgaacatg taggttctga aaatcttgaa ggttatttca aagtggtaaa
    41281 agatttattg aaaccaaaag gaacagcttt gatccatgga atcagccgac aacaaggcgg
    41341 tgcgacaaat gcttggatca ataaatatat tttccctggc ggttatgtcc ctggtgttac
    41401 tgaattagtt gataatatta caaaaaacga tttgcaactg attgatttgg aaagcttacg
    41461 tcgtgactac caattgacat tagaacattg gacaaagaat tttcatcaag tccaagatca
    41521 agtcacgaaa gaaatgggtg attccttcta tcgaatgtgg gatctatatt tacaagcttg
    41581 tgcagcttca tttgaatcaa gtaatatcga tgtgattcaa tatttgcttg ttcaacctgg
    41641 aaacaatgac atcccaatgc atcgtgcata attgaggcaa aagtactgtt taaagactat
    41701 ttaaaaagaa gctaagtttt atgatgctcc agaagtgaac ttttttgtct aacttctagg
    41761 attgtctcta aagcttagtt tctttttctt tatattttca taataaaagt ttggttcttt
    41821 aatttttaaa catgacagaa aattttttct tcgttatact tgtaatagaa tactatttta
    41881 taacaaaggg gatcgaatca atgaaaaaaa ctgttattta tactagttta tttgcagctt
    41941 tgtttttagc tgcttgtcaa tcaaatacca atcaaggcac atctgggaat acgtcttcca
    42001 gtagcgaaca gcgctcttca caggtatcgg actcctcaac aaaagcaagc acatcttcgt
    42061 ctagccagac aaaaagttct agtgaagcaa gtactcagtc agatatcaaa gtgtctgcag
    42121 ctcgtgcgat cgaattgttc caagataaat acccagatac tgcaattact agtttggaat
    42181 tagatagtga ttggggatct tacttctaca agatagaagg tgtcgatgat caaaaagaat
    42241 acgaagcaag aattgatgct caaaatgaaa aaatggagac agagaatcca gaaactctag
    42301 atcgagatga acaaaatggt caaaagaaaa aagaagatgg tctggatgtt gataaattga
    42361 tcacgattga ggaagcagca aaaatcgccg tcgacaaagt aggaagcggt acggcaacag
    42421 attgggattt ggataaagat ttaggtacta cttattggga agtcaaagta aaagacggca
    42481 atcaaagtac atcagtcaag atcaatagcc aaacaggcga agtactgtca actgaacaag
    42541 atgattaaga atttcttcgg aaaacaagag ttgatttttc ttttagtcat gatacactag
    42601 acaaaataac ttgtttgaag gagcgattta cttgagttta gaaacgacct ttgccaatca
    42661 tatctttgcc aatcctttga tgaatgcatc tggtgtccac tgcatgacta cccaggaatt
    42721 agatgaactg gctcattcag aagcaggagc atttatcact aagagctgta cgatcaatga
    42781 acgaaaagga aatcccgaac cacgctattt tgatgttcct ttagggagta tcaattctat
    42841 gggacttcca aacttaggtt tttcttatta tctagagtat gctcttgcgt atgaaaaagt
    42901 gcaggaaaac caaaatcaac cgttattttt ttccattgcc gggatgagtg tccaggaaaa
    42961 cttggaaatg cttgagaaaa tagaaaaaag tgggtttaat gggatcactg aattaaattt
    43021 atcttgtccc aatgtgcctg gaaaaccaca actagcttac gattttgagg ccacttatga
    43081 aacactgaaa gaagtattca gcatcttttc taagccgcta ggaatcaaat tgcctcctta
    43141 ttttgatttt gctcattttg atcagatggc tgatatcttg aatcagtttc cattgaccta
    43201 tgtcaatgct atcaacagtg tgggcaatgg tttgtatatc gatacagaac aagaagcagt
    43261 agtcatcaaa ccaaaagaag ggttcggcgg cattggcgga gagtatatca agccgactgc
    43321 tttggcgaat gttcgcgctt tttatacacg tttgaaacca gaaatccaaa tcattggtac
    43381 aggtgggatt cggacaggac aagatgcatt tgaacatctg ctatgtggcg caagcatgtt
    43441 acagattggt accgagcttc ataaagaagg gccggaaatt ttttctagaa tcataaaaga
    43501 attaacgcag attatgtcag aaaaaggata tacgtcaatt gacgaattta aaggcaaatt
    43561 acgcaccatt tcatagcaaa gagacgaagg tcatgattga tcttcgtctt tttttataaa
    43621 aaacactcgt ctgttcattc ctgtgcaagc gtactcattg tatgttagac gaacttgtgg
    43681 gaaaataaca gaggcaaaaa ggaggataat atgaatgatg caacagaatt agcaaaagag
    43741 cttaaagaaa aagaatttag ttttcaagaa ctagttacgg atatacagaa aaaaataaag
    43801 aagcaaaatc atgaattgaa tgcgtttgtc gcgcttgaac cattcgagag taattcccac
    43861 gagctattga ttccccagtc agaaaaaggg ccattggctg gtatcccatt tcctttaaaa
    43921 atgctcggtc aagaaaaaaa gggatggcta gctacttcag gatctcgttt atttgaaaca
    43981 caccgagctt ctcatacatc taattatgtg cggcaagcag aagcgatagg tctagttcct
    44041 tttggacaaa ccaatgcgcc agagtttggt ttcaaaaata ttacagaccc agtcatctat
    44101 ggcccagcaa gaaacccttg gaaccttgaa tgtacaccgg gcggatcaag tggaggagct
    44161 gctgctgcag tttcatcagg aatcgttcct ttagctggtg ctagtgatgg cggtggttct
    44221 attagaatac ccgcttcgtt ttgcggattg attgggctga aaccttcgcg agggacgatg
    44281 ccagttggtc catatgcttg gagaggatgg caaggagctg ctattgattt cggcttgaca
    44341 gtttctatga gagacacgga ggctttattt tatgggatga gaagtatcca ttccggtgcg
    44401 ccatatcaag tatcgccagt tgagtggcaa acacatccaa gaaaaaaacg gttgaagata
    44461 gctttttgtg ttgattcacc gatccactca cagattagtg aagaggcagt acttgctgta
    44521 aaaaaagctg tagatttcct tggaaaagaa gggcatgatt tgacagagat tccttaccca
    44581 ctagatggtc gtaaactgat cacgtcttat tatcagatga atggcgcaga aacagcagca
    44641 atgatccgct cgattgaaac aggcatccag cgagctgtca gaaaagaaga acttgaaccc
    44701 atttcttgga ctttgttcca atatggtcag aagctctctg ccgctactta catcgattct
    44761 ctgcatgtat gggatgaagc agcaattgtc atggaaaacc tacatcaaga atacgatcta
    44821 ttcctttcac caacgactgc atttaccgcc cctaaaatca gcgaagattt gcaaagtgat
    44881 gcgataagag cacagatgga acacatcagt gattgttctg aagtagaagg tttagacgtt
    44941 gtttcttcca tgtttgagga gagcttgaag atcacacctt atacacagtt ggctaatctg
    45001 actggacaac cagcaatcag cttgcctact catttgacaa aagaaaacat gcctctagga
    45061 atccaattta tggctgccag aggaagagaa gatttgctat ttcaaatcgg ctatctattt
    45121 gaagcaaatc atcagttcca tttaccaaat ccatggaaat agcagaaaca aggagaagaa
    45181 gaatgaagaa aaaaagattg tttgctctaa taactagtgc cttttttatt ttaagcgctt
    45241 gcggtacagc tgccgctaat caggaagata acagctggag taatgtagaa aaaaagaaag
    45301 aattagtagt agcgacttct ggcacactgt ttcccgcatc ttattataat gatcaaaatg
    45361 aattagttgg ttatgatgta gatttggcaa aagaggtggc taaacggtta aatgtcaaaa
    45421 tctcctttaa agaatacaac gtggatggac aaatcacttc tgtttcaaaa agagaatcag
    45481 attttgctgc gaacgatttt ggtatcacaa aagaaagaga aaaaaaattc tccctttcag
    45541 agactttcaa atattcattt gacagtatga tcgtacgaaa aaaagatgat tcaggaatcc
    45601 actcacttga agatcttaaa gggaaaaaag cagctggcga acccaatact agctatatgc
    45661 gtttagcgga aaaacacgga gctaaattag tcacatatga caatgccacg aatgatcagt
    45721 atttaagcga tgtagcaaat ggtcgaacag atgttattct aaatgattat tatctgcaga
    45781 aaatggccgt atctgcttta ccagatatcc ctgtaaaaat actggagaac gtttatttca
    45841 atgaaaacga aagtggtttt ttattgaaag aaggaaatga tgctctgaaa aagaaaatcg
    45901 atggcgtatt aaaagagatg aaagaagatg ggacgacaaa gaaaatatct gaaaaatatt
    45961 ttcaaactga tgtttcagtc aaaccgaaag aaacgatggc tgagtcggtg agtgagtgat
    46021 gtactttcct ctaattgatt tttccctcat gtggacttca cttcctttcg tactgcaagg
    46081 cttggtatac acattaggga ttggcttcgt gtcgtttctt ttaggaaatc tggtagggtt
    46141 gcttttgaca gttttaggtc ttttggattg gctgcctctg aatgtattta ttcgttttta
    46201 tttatcgttc tttagaggca ttcccgcact agtcctgctg tttctgttat attttgggtt
    46261 accttatcaa ttaagtgcct taacggcttc agtcatctgc tttacgatta ccagcagtgc
    46321 atttattggt gaaatctatc gaggatcact agcaggcgtt tcttcaggtc agtgggacgc
    46381 tggtttagct tctggttttt ctttctttgg ggtattgaaa tatatcgttt tgccgcaagc
    46441 ttttcgaatc tcagtaccgg cactcagtaa cgtagctatg gatctagtga aaggtacttc
    46501 gttagcagca acaatcacag tgccagaaat atttcaaaaa gcaaagatca taggcggtcg
    46561 gacattcgat tatttttctc tctatatttt agttgcactg atttattggg tcatttgtat
    46621 cgggatcgaa aaagtgcaac agtatttaga aaaacgttct atgcatcagt caatctgaaa
    46681 aatagaaatg gtgctagcgt ggctggcacc atttctattt tttgtttctt gtatattctg
    46741 cctagtattt taaaaccatt tcatgtaaaa tgtaaatcga aaatatgttc ggaggtagaa
    46801 aacatgaaga ccccgctaac catcgaaata gaatcctcgt tataccaata ttgcatcgag
    46861 caaggcggct tggttgtaga agaagtcacg atgcctgatg accaaggaat tgtagatacg
    46921 cttagctgct tgacaaaatt tgatggaagc cgagaatggc gatgctatga gctgaaagtg
    46981 acaaaagcag attttcgctc tacagccaaa ctgtcatttg ttggacatta taattatttt
    47041 gttttaccaa gtaaactata tgaagaagta aaaactgaaa tccctacagg aataggtgtt
    47101 ttagtgtatc gtccgtacac ggttgaagag gagatgcctg cctctggtac atttctgatt
    47161 gcgaaaaaag cagcaaagaa agagcttgct gtaccggaag aagctttgac aaaccgcttc
    47221 atggcttctt tattcaggga agtaagaaaa gcaaagcaaa tggagcgagg cgtaaaatat
    47281 tttccaacag atcaattgta caaagaattg aaaagacgtt cagaaaatca agatccattt
    47341 tctaacgaac gatattatga acggttttta gaggaaatag ctagtcaggc aatcaatgag
    47401 ttgcaagaag aagttatcgc gcttcagcat gattatgaat atttgaggca acaacagcag
    47461 atccgtcggt tgcctacaga gccattggag tgaaaaatat gtactatcct tatttaagag
    47521 gaaaacaatt tgacctatta gcattaaaag aagcactgtc gcgaggattg ctctccaata
    47581 aaatccaacc agtgatcgaa ccagtacgtg attccgctac gcttaaaaat gtgatcgaat
    47641 tatttcaaaa aaagcaccat cgaatcgctg tgatccaaaa tccccaagta ggacaattca
    47701 aattattcga tcagcatgtg cacagttgga cagtcaaaga aaatagctct gtcgtccctg
    47761 cacagatctt aacgccagaa aatctcagtg aagtactgga ttcacctcca gcgtttcttg
    47821 ttttcgatgg acagcactac ccaaaagata cagaggtgtg gaaacagttg gcaggagttg
    47881 attcgaaatt tttgatacca gatactagtc gctttcgcat ctggctgccc gaaaacaaaa
    47941 ttgtgatccg tgactctttt caaacgagaa agcatgttga aagttatgca gataaaacag
    48001 atgacttttt tagtgatgac tatctttttt tccacgcaga cggatatatt ggattttctg
    48061 atttcacgat cgaaggtagc cgttatttcg ataaagggtt ccctagccga gcgcttgcta
    48121 tccatctaac gtatattgat gcttacggca atatacgcgt gaaacatttt gtctctgaca
    48181 gtaacgactc tgctaaagac caagcattga agtttcttga agcaggagat aaaatgaaaa
    48241 aatggatcat gcgtcaccat catcaactct tgattacttc aggaatgatt gaattattgg
    48301 ctctttacca gcaacaaaaa ttccctggac tgggagtgtt gaaaaagtgg tcacttatgc
    48361 atcatttgga acttattagt cacctgctag atcatccaaa agattggctt aaatattatt
    48421 caaaagttca ggaactatat gaagtgatcc aatgattaga taatgtaaat gaaaaaggaa
    48481 tagaaaagag gaaactggat gtatattcgg caaacaagga aagaagaagt agaagaagtg
    48541 gtagaaatca ttaatgccgc agcggatttc attgcttcgc aaggctctcc tcaatggcaa
    48601 aacgggcaga aaccaacaaa agaaacgatt ttgaacgata ttgaaagaaa agaaagctat
    48661 gtattaatag tggaagatca aattgctgga acagctgcat tagtctcaag cgtggatccc
    48721 gtttacacaa aaatagatgg tagttggaaa gaatcaactg gtccttattt atcgattcat
    48781 cgtgtcgctt tagcagaaag tactcgagga aaaaaactag caaaaccgtt cttagaacat
    48841 ttgttgacga ttgcagccag caaaaatatc caagatatcc gtatcgatac ttatccagct
    48901 aatgaaccga tggaaaagac catctattct gctggattca cttatcaagg gatgattgaa
    48961 tttccttttg aacacggcga aagaaaagca tatcaaataa tcatcaattg actataaaat
    49021 catctgagca aagataaaga cattaacact actgaaaaaa acaatcatat gatagccaag
    49081 aggttgtaac agaagtggcc agcttcaaaa aacaagaaag aatttccgaa aatgcttctc
    49141 aaatttttgt ggaattttag cgtattttac gaaatagctg ctttctgaaa ctcccttcat
    49201 cgagaggtcg tgacatagct tttgtcacga cctctcggct tatcctacga gtattattcg
    49261 atacatttat atcaataatc ctttttttat tttatctgca gcttcttgga acgctccatc
    49321 gtttgacacg actaaaagtg aggggatagc tttcaaacgt ttcaactgtg ctaaatcttc
    49381 tacataaacc gccagtctgg ctgaaatctg ttcagatgaa tctccacgtt ttttcaggcg
    49441 cgattgcaat gtttctttag agatcgtaat aaagatagga cggactaccg tcccgaaata
    49501 ctcatataaa tgccaaaaac catttgctgt caaaacggcg taacaatttc ctactgtcag
    49561 tttcttttta atttctgctt tactggaacc atatttatgt tgagcatatt ggtcccactc
    49621 tgcaaactca tttcccttga tcatctgatt gaatgtattg tcagaaacaa aataataatc
    49681 ctcttgatct ttttccccag gtcttggtga tcgattcgtg taagtaatga tcttttgttc
    49741 cggctgaaaa atagcttctg cgagtgaagt ttttccagaa ccagaaggac cgataaatac
    49801 atagcaataa ttcattgtca tttctccttt gatgattgaa caagtgaaaa agctttggaa
    49861 aatatttcca aagcttttaa aagtaacttt gcttttttgt atattatacc agcaagccag
    49921 tcacaatagc acttaaaaag ctgactaaag tcgctgttag caaaattttc aatccgtgtt
    49981 ttgcgacaag atttcctttt tcttcattta atcccttcat cgcaccagaa atgatcccga
    50041 tagaagagaa gttggcaaat gaaacgagga agacagatat aattgctgtt gtacgctcag
    50101 taaaatggaa attaccattt gctaattctg tcatagcaac aaattcattt gtaaccagtt
    50161 tagtcgccat gatactaccg gcatccactg cttctttcca tgggattccg ctgatgaagg
    50221 caagaggggc aaagatgtaa cctaggatct cttggaaagt gatgccgaat atcccgtgga
    50281 agatagcatt gatcatggca atcaaagcaa caaaaccgat aagcatagca gcaaccgtga
    50341 tcgccacatg gaaaccatca agaatgtatt ctcccaacat ttggaaaaat gtctgttctt
    50401 tgttttcttc aatgatcagc tcatcttctt tttcttctaa tacataggga tttacaattg
    50461 aagccagaat ataaccgcca aataagttca aaacaagggc agtgatcaca tatttagaat
    50521 cgatcagtgc catataagat ccgacgatcg acatagaaac tgtagacata gccgatacac
    50581 tcatggtgaa caaacgtttc tcagatagga agggtaattc cttttttatg gaaataaaaa
    50641 cttccgattg tcctaaaata gcagaagcta ctccattata agattccaat ttgcccatcc
    50701 cgttgatttt acttaatcct agaccaacag ctttcataaa taatggaaga atcttgatat
    50761 agcgtagaat accgataata gcagaaataa atacaattgg catcaatacg gaaagaaaga
    50821 aaggtgcact tcctttattt gctacaccgg caaaaacaaa gtcaacacct tgcccagcaa
    50881 aagcaagaag atgatcaaac atttttgcca atacagaaac aaccgctgta ccaaaagttg
    50941 tacgtaataa tacgaaacca agaacaagct gtaaaacgaa catcacaatt agtggtttca
    51001 atttgattgc ttttttgtta ctgctgatca aaaaagcaag ccctaatgtg acaaataaac
    51061 caataatacc taataaataa ctcaaataat cactcctatt ccaaattccg ttttatttta
    51121 cctgttttgc aaataaaata ctaatcttta gtgaaatttt cattactttt ttagagtgaa
    51181 acgcattcat tttccattag aaaataaagc gaacaaaata aaaggtttaa aaagtgtaac
    51241 gtcttagtaa agtctataaa aaaacgaaac tatcgattaa gctaatagtt tcgttttcaa
    51301 catttataag taaacttttt ctattggtaa tatagatttt cggaagggta gacaggtgcg
    51361 caggtaatat cgattcctag ttttttcaat gtttgttcat catttttatt gaccatcaca
    51421 gtagagtggg cttgtgctcc ctcaagttca gctagtttat cataagcaac ttgagcgatt
    51481 ggattcgtta ctgcactgat tgataaagcg atcaagactt cattgatgct tagtgcatcc
    51541 aaagaggaat gaagtgtctt tgatttcaat ccttgtattg tttcaagaac taatggtgac
    51601 aataaaggaa tgtcatctgc gatatgggct aaatgtttta ctgcatttaa gataactgcc
    51661 gctgacgcat ccatcaatga acttgtacgc ccggtaatga tgacttcatc tggtaattcg
    51721 attgcaatca cagcagaagg atcttttgta ggactttgcg cttgaatctt atctgcatag
    51781 tcatgggcag ggactaccac gtttcggtct tcaggacgta aaccgacttc ttccataatc
    51841 agtttgattc gattaactgc ttcttcgtcg atcaatcctt ttttgaagtc gcattctgtt
    51901 tggaaataac gacggatgat ttcttgttta gaggcttctt ggacaacggt atcatccacg
    51961 atcccaaatc ctacacgatt cacacccatg tcagtaggag attgataaac agattcttta
    52021 ccagtaattt tttcaataat acgtttgatt acaggaaatg tctcaacatc ccgattatag
    52081 ttcacagcta ctttattgta ggcatcaaag tggaaggaat cgatcatatt gacatctttt
    52141 agatcgactg tggctgcttc ataagcaatg ttcagcggat gtttcaacgg aacattccat
    52201 actgggaaag tttcgaactt ggagtaacca gctgcatgtc ctttttggct ttcatgatac
    52261 agttggttca aggaagtagc gagctttcca cttcctgggc caggagctgt cactacaacg
    52321 attggtttag ttgtttcaat atactcattt tttccaaaac catcttctga gacgattttt
    52381 tctacgttga ctggatagtc ttcgatttct gagtgtttat agaccttgat gtttcgtctt
    52441 tctagtttat tgataaagac tttcgtggca ggctgtccac tatagcgagt gataacgaca
    52501 ctgttgatta ataagccata accacgtaat tcatcgatta accgcaagat atccatatca
    52561 taagtaatcc cataatcacc acggatctta ttacgttcaa tatctccagc atagacgcaa
    52621 atcagtattt cagcttgatc tttcaatttt tgcagcaatt tgattttcgc atcttcttca
    52681 aatcctggaa gtacacgttt cgcatgcttg tcgtcaacta gcttcccacc aaattctaaa
    52741 tataatttgt catagtggtt cactcgttcc aaaatatacg ctgactgctc ttcaatatat
    52801 ttttctgaat caaaaccaat ttttttcact aggttccctc cttaagtact aaagcatttt
    52861 ttcatttttt tgaggattcg tcaactggaa gagcgaaatt cctcaaaaaa acaaagaaaa
    52921 tggtttaaac caacttgtct agatagttat tcattggtat gatagttttt gataaggaag
    52981 gtgctacatg aaaaaaagac agaaaaagaa aaacgcatat aaacactata tccgttcgat
    53041 ttttaccggt tatgaaagaa tgcttgaaga tcccgaattg gaacaattga cgtttactta
    53101 tctaaacgaa gaaacgcagc taactcgtga tgagcatcag cgcattcatt tcacaacaag
    53161 agatctgcct agtaaataaa aaataagata aaggtcatcg gattcgattc tgtcctatca
    53221 gatggaaaga ctagttgatc gaaaaaaagg ctgtaccaaa ttttggtgca gcctgtttta
    53281 tggagcattg cccgctcttt tttataacaa ttttttacag aaaatggtac tattgaagta
    53341 ttaataagtg aaaaacagat gagtaagagg aggcaagtag tatgaaagag ccagatacaa
    53401 atgcaaatta tgagccagac gaactagagg agacgaatgc ggatcaggat acaggacaag
    53461 aatcgtcttc tttttacgat atcaaagaaa aagcaagcat gaatctttcc caattattag
    53521 aagacttaca ggcttttgaa aaagctgtcc gcgaagaaga tatccctgag atctaccgtt
    53581 tatacaatgg ccgattgaat gaagaattaa aaagaagctc aaatgaaaat cacgagatcg
    53641 atcaattgct gatgcgtaag atccatgatc gttttttaca ggaatttcct tttatggaac
    53701 aagtagaaaa catctcgcca acgatgagct attacaaaat cggcacttat taccatgatc
    53761 ggcccacggt cggtattgat gcaagtctgc cagaaatttt tgttttgcct aagatcgatg
    53821 aagagtggga gaaatattcc caaccggagt caacggacta tgacaaaaaa atcaatgagc
    53881 tagacgcaaa ggtgatcact gctcagacag aaattgaacg tttagatgaa caaataaaag
    53941 aaatcaatcg acaaatgact gatttgaacg acaataaagg attcttgaat cgtaaaaaaa
    54001 tcgaggaaga aattcaagaa ttagagaaga agaagcaagt actatctaat gaaaaattag
    54061 gctggcttcc ttatattgag acgcctgaaa caatccagca gcaaaaagaa gcattgaagc
    54121 aagaagcaag agcagaccag cttcgtgcag ccatcgtaga aaaagaacaa aggcaaatca
    54181 gacgctattt cggtagcaaa gagggattcg gtcaagcaat ccatgaattc ttgatgaatt
    54241 atcttggcaa tgagaatcct aataatcaat ctaatgaagg aggttccgag tatgaatgaa
    54301 cacgatgaag cagaatggac aaaaaatgaa gaacaaacag aagatcatga agaaacgaac
    54361 acttcattgg aagaacaaac aagtaatcct ctggatgaca cagaagaaat ccctatgcta
    54421 gaggaaacaa aaatacacga aaaagaaaaa gtaactccga cgaatacgaa tactgcagaa
    54481 aacacccatg cttacgaaat aactgaaaat cccgggtatt tagaaggaaa agagcaagat
    54541 catcaagata tcgtcagttt gattgaaaca gcggaagcag aattagcgga tttacgtatg
    54601 agaaaagatt tgatcgaatc agattttcca caattattag aatcatatga tcgttttata
    54661 ttgaatgaag aaccaatctc cacgatcgga aagacaagcc tgatcgaata cttcacgtat
    54721 gaattactga aaccgttgaa tgatattggt ctggaacggt caaattcagc ttatgacaca
    54781 tggaaaacaa gacatttttc gatcaattat acgttgaatg gacaaaacga attagtcttt
    54841 tcctttgtat taccgaccat cgatcaacga taccaagtgg aatcgatgag tctcctaaac
    54901 gtatcaccag agacaatgga aatcaatgtc caagatgacc gggtgctttc tttgatccga
    54961 cactggtcag tggatcgagt attctctgca gggcagatca cgatattcaa tcacaaattg
    55021 aatcaattat tagcccatgc aagaacactt ggttttaccg ttaatcagac attactagac
    55081 aatacgaagc cacttcgttt gaatcttcag agcgaatttg aattgacgaa tgaagtattg
    55141 gatgatattt ttatcgtggc aatgaaaaat ccatcctacg atatggaaaa gatcaacgaa
    55201 actacttatc aagtacgatt agataaagga cagtctttaa cgataacaac aaacgaaaaa
    55261 gatcaaacag tcttgtcgat ctcatctgga gactacccac gatctgtcat tgatttcttt
    55321 atcaattacg aattcttggt cccattaatg gtgagaaagt cctgattgga taagttggat
    55381 taccagatgt cattcagata attctgttcc aaaggacttg cggcagttat ccagaataat
    55441 tgtcacaagt cttttctgta tgtactaaat ggacgtgaat aacttgctga tgaaggaggg
    55501 atttcattag gaatgtccac aaataaaata gcacagctta gcttattgtc tgctgcttgt
    55561 gtagcaggta ggatcgcttt tcaatttatt ccgaatttcc agccaatgac tgctattttt
    55621 ttatttatag tactttactt aagcttaaaa gatgcgttaa ttgtcatgag tctttctatt
    55681 gcgatcagca gtttttattt tggtgttgga ccatgggtca tcggccaatg gatcagctat
    55741 actgttgttc tgagtttgtt ttcaatcgtc tgcttgagaa aaacagtgca aaggaatcta
    55801 tggttaaagg gagtctgttt ttttctggcg ggcatggttt acggaatagg tatgaccgtt
    55861 tttgatacgc tgttatatcg tttgcctcaa ccttggatct attaccttca aggggtgtcg
    55921 tttgatttga tgcacagtat aggcaatgtt gtgttctttc ttgtttttct gccagtcgtc
    55981 aaacgttttt acaatcattc aggaggaaaa aatgaaaaaa ataatttata gtttgcttgc
    56041 aatgagtgtc agtatactca ttttgacaag ttgcagtaca acctatacac aatcggcttc
    56101 caaccaaaag gaggaagtaa aaacatcgca aaaagcaaca atcacgttac aagaaaatgg
    56161 tcaaaacttt tcggaaaaag aagtgttctt caaaaaaggc gatgatttac tgagtatttt
    56221 gaaacgaaat tttgaagtca aagaagataa cggcttcatc acttcgcttg aagggcactc
    56281 acaggatgag aaaaatcaac tctattggat gtttactgtg gacgggaaaa gtgctactac
    56341 tggtgccaaa gagatcaaat tgaatgatgg acaatcggtc attttcaatt tgtctaaact
    56401 ataagtttca taaaaaactg ttaaagggcg aatattgcca taattcagtg agttcagttg
    56461 atgaaagaag gaaaataaca tggaaaattt taaaaaaagc ggaatgtttt ccgtaattgc
    56521 tgctctaggg gctaatattc tagttgctat cagcaaattt gtcggctatg caatcagtgg
    56581 cagtgccgcc atgctaaacg aaagcatcca cagtatcgtt gattgcagca atcagatttt
    56641 gctgttattc ggtgataaac aagccaataa aggacagagc gagttgcatc aatttggtga
    56701 aggacgagca aaatactttt tcagtacaat cgtcgcaatg atgcttttct tcggcggtgg
    56761 ggcacttggg gtgatggaag cttttgaaaa gttgacgcat ccatcccatg aagtgggtaa
    56821 tccgatcatt gttatagcaa tactgttatt tggtttggta atcgaaggaa gttcgttacg
    56881 cgtggcaatg aaagaaatca aagaattgaa tactgaaaaa ctttccacga tgcgcttttt
    56941 gcgcgaaagt cgacacagtg aaatactgat tatttttacg gaagacttgt gtgctgtaat
    57001 cggtttggtc ttggctttag caggaacaat attgacttat gcaaccggga ttgctgcatt
    57061 tgatgcgatc agcggattgc tgatcggttt cttgctgatg ggagccgcca ttttcctagc
    57121 aaaagaattt tatagtctga tcatcggtga aagcgtgaca gcaagcgatt tatcaaaaat
    57181 caagttagct tttgaacgtc ctgagatcag tcggctgatc gatataaaaa cagttcattt
    57241 aagtccaacg gagattttag ttgcagcaaa agcagatatc gttggtaaca aagagaatga
    57301 agcttactcg gtcatcaatg atattgaaaa aaatatgcgt cagtctcttc ctgacaagaa
    57361 gatgtatata tacattgaaa cagataaata tgacccaaat tattctcgta aataaaacgt
    57421 ttattcatga attgaagcaa aaatgagaaa aatataatct tgtttttaaa taaaacggct
    57481 acaatttagc gaatttatgt tacaatgagt ccgctgttta tttgttgtaa ttaaaggaga
    57541 tattttaatg aacaaaaaga aaagtgcgcc atggcttcaa gctttgcatg cagtgatgcc
    57601 actttgtata agctatatac cagtaggatt agcttgcgga gtattgttgc aaaaagttgg
    57661 atttgatcct ctcttatcag gactgttatc gattctggtc ttttccggtg gcgcgcagtt
    57721 tttagttgca tctatgctta cgacgcaagc atcatttgca acgacattat tgatggtttt
    57781 ctttttagaa ttacgctata cattattagg agcaagtctt gccggtttta tgaaaaaaga
    57841 aaagcggacg tttttggctg tcttttctca atcactgaat gatgaaaact atgcggtgaa
    57901 ctacttaaag ttttcaactg acccttcctg gaataaaaga aaggctttat atgtcaattg
    57961 gttctcaatg actgcatggg ccggtagcaa tatgatcggt aattttttcg gctcggtgat
    58021 tcgagtagat gctgaccttg tccattttgc tttgacggcc atgttcattt tcatgtttat
    58081 catgcagatg aaaagcgcac tactgatctt tacaggttta ttctcaggcg tattaggggt
    58141 cgtattcatg gtattattcc aaaatacttt cggattgatt gcagcaacag tcattgcttc
    58201 atttgccgga tttatgctgg agaaggcatt caaaaaagaa aaagctaaga aactaagaga
    58261 ccgcggtaaa gatgtcaatg agccgctctt caacttcccg gaacaggagg ccaatcacca
    58321 tgactaaact atatttgcta cttttagttg cttgtctgtt catcgtggca tatatcccgc
    58381 gcttgttccc gatgctttat tttactcatc gaaaagtgcc atcctggttc agtgactgga
    58441 tgaaatatgt accggtggca ttatttgcgg cgttagcttt taaagatgtt ttcattactc
    58501 atgaacatct ggatattgct tggaatatca aaatagctgc aatgattcta gtagcaggcg
    58561 tggcttacaa aacacgttcg atggcgttgt cagttttgac aggacttgcg tctgtctttt
    58621 tgctgtctat gctttaaact gttttatttt attaaaaaca gagattgcga cataagttgt
    58681 tcaaactgat ctatccgaac tgcatttgca acagtcaatt ctggatgcaa gtttattcgt
    58741 agggataaaa aagattagtg ttggcttatg atgcaagctc ttttttattt taggaattag
    58801 agagggattt gtccttctat gattttttgc tataataggg aagatgttta ttttatgaaa
    58861 gaatagttga gggaatttaa atgaataaaa aattgatgca aggaacattt tggctaacct
    58921 ttgctaatct tctatgtaaa gttttagggg tagtctattt gattccctgg ctaagtatga
    58981 tggggaacaa tcaagatggt atgcttgcta cgacattgta taatgttggc tatcttcctt
    59041 atggattgtt cctgatgctt ggtacagtcg gatttccaaa tgcgattgca aaaaaagtag
    59101 ctgtagccac aaaagaagga gataacgctg cttgtcggct gatttttaga agtacgatca
    59161 atatcatgtt tgtcatcggt atcgcctctg ctgcactgat gtatttattt gctcctttac
    59221 tagccggaat cagtccgatt gctaacgtta ataatgggat actagctatc agaagtttat
    59281 gcccatcttt gattgctatc ccaattctta gtgcaatgag aggatatttc caaggaaaaa
    59341 atgatctgcg gccttatgga acttctttga ttatcgaaca ggctgtgcga gtgatcgtta
    59401 tcttggctgg tacttattac ttgcgcgtat tgactgacgg aaccatatta gaagctgtat
    59461 tgatcagtac agtcgcttct ttcttcggtg ggttagctgc gattatccat atgtatatcg
    59521 tcggacgcag aaaagacttc ttcgaactta aagatttttt gatttccagc cgttatttcg
    59581 aaagagaaaa ccgatctgcc tctgtctcta tcattcggga aacactaccg tttatttttg
    59641 tcggctcagt cattactatc gttcaaatga tcgaccaagt aacgatgaaa ccactgcttc
    59701 actttttccg tccagagatc gcagatcagc aactggaatt cttattcagc cgtgcttctg
    59761 tcaatccaaa taaattgaca ttgatcctga tctccatggt aggaacagta gctataagca
    59821 gcttgccaat attaagcacg atgaagaaaa aagatcggat acaaatcgaa aaaacagtag
    59881 gagatagttt ttcgattgcg ttattgattc ttcttccttc attgactgga atgtctttat
    59941 tagctggacc actatacaca ttgttttttg gctatgatcc tgaaagtgtc ggatatttcc
    60001 aaatggcttt gctagcttct ttgtttttct ctttattcac gatcctatct acgatgcttc
    60061 aatctttgaa tcaccatcgt tttgcgatcc gattaactgt agaagcaatt atcctgaaag
    60121 tcatcttcca agtaatcgga ttaggattag ttggcggata cgggatgagc ttatcaaatg
    60181 gcttagcatt cggtattgtc tttgttcgcg gctatcactt tatgtgcaaa gaataccgaa
    60241 tagcaccttt agcaaagatc agcaattttt tcttaaaaac tttccgctcc accctgatta
    60301 tgcttgcagt atgttttgcc gttttctttt tgctgaatca gcgattgtca atggaatcaa
    60361 aaagctatgc aatgatttat tgtgtggctg taggaagtat tggcggctta gtatttttat
    60421 tttctcaatt cggaaaaaat gggtttcgaa tgttgaaaaa tgttaaacat catagcagaa
    60481 gcaaagaata ggttaactaa tcgttttttg ctgtcacttg aaaaacggca actcgtatga
    60541 ttacgcttgt cgtttttcta ttttccaatt ttagtttcat atctaatctt acttatgata
    60601 tagttataag gtctggaaaa attctttgaa acgaggaaat taaacacatg aaacttgcag
    60661 tagttacaga tagttcggca tatctgccgg aacgtatcaa gaaccatccc gacttattcg
    60721 ttattccaat ccctttgatt atggatggga aaatttataa cgaaggaatc gatatcgaag
    60781 cggatgaata ttatggactt ttaaaaaaca gtaaagattt tcctactact tcacaacctg
    60841 tcgtagggga agttttgggt ttatatgagg aattgaaaga gaaagggtat gacacgattt
    60901 taagtatcca tctttcttca gggatttctg gctttgtcaa cacattattt gcgatgaaag
    60961 atgacatcag cggagtaacg gtcattcctt atgactctaa gatcactagt atgccgatgg
    61021 gacacatggt agaagctgcc ttagacttaa atgatgaagg tcagtccctt gatgaaatac
    61081 ttgcacatat cgatcgaatc cgagataata cctacgctta tctgattgta gatgatttga
    61141 acaatttagt acgaggagga cgtttgacaa atggtgcagc gctgattggc agcttattaa
    61201 aaatcaaacc gatcctgaca ttttctgaag ggaaaattgt tttattcgaa aaaatccgtt
    61261 caagtaaaaa agcatttgct cgagctgaag aaattattgg tagaagaaat aacgaaatac
    61321 aaccgccagt caaactttac gtgatacatg caaataattt agaagtagcg attgcggaga
    61381 aagagaaatt gcagaaaaaa tatccaaatg cagaaatcga aattggtcat ttcggaccag
    61441 tcattggtac acatttaggt gaaaaagcca tcggcattgc catttcagcg caataattcc
    61501 ttttattcaa aaaacatacc aaaaatattc caacatcatc ttggactttc tacatgttac
    61561 taatgagcgt gtaaaagaac gagatggtgt ttttatttct atcaatgaag ctctttttct
    61621 ttgcaatcct cacttcaatc ctctataatg tcgcatatag tttagtaaat gagtaaacta
    61681 ctaatttact tatttactaa aaataaaagg atgaggagtt aaaataatgg aaataccaac
    61741 aaatttaaaa cataaaccag taatcgttgt agaagactat gataaagttg atggaagaaa
    61801 tgcattaaat acggatgcaa aagggctttc actgggcctt gctcagtgga atgatcgtgg
    61861 aaaagtcgat atctcaggca aggtatggcg acatactgga gaaaaatggt cacgacagtc
    61921 ggaagaatta ccaatcacac gtattttaga tttagcgatc ttagctgcac aaggaagttt
    61981 atacttccaa gatgcttatc gttatgaaaa attttatgat ccagaaaatc cagtcgtaga
    62041 catcattgga ttacaaggga accgaatgac ggtagaagta gataccaaaa atccaaagat
    62101 cgatgaagac atcattttgt attacgatac actacaaaaa gacggagaac tgattgggga
    62161 acgattccgt attttaaaac gtcttttaga tgagctagga tattaataga cagagatgat
    62221 caaaagggga agagaagcat ggagaatttc tcactaacag aaaaccgaac cttttcacgg
    62281 ctagaaaagc aaatgatctg gaagaaaccg aaaagccatc ggacaagtgc agaagaatgg
    62341 cggatcgcct ccgaaatcaa agggaattgg caagatcctg aaatgaaaat cagtaacgta
    62401 cttttggaag gagatgccgg gtcggggaag acacaattag ccaaagcact atctgctgat
    62461 ctgcaacttc cctatacaaa agtgacctgt tttgcagata tggacaaatc agatgttttc
    62521 ggagccttat tacctgtagt agattctaat aaggaacaag accaagaatt actagaagct
    62581 atttaccaaa cagatacact tgaagctgta ctgacactga tccagaacca ttacggcatt
    62641 gatccttttt ctgctaaaga aaaattagca gagctagttc aaaggatcga agctgattat
    62701 ccagatgcaa tccattacag ctattaccct tctgaaatcg ttcgagcgat tgaaaaagga
    62761 tatttattag agattcaaga gccgactgtc atccgagatg cctctgttct tgtggcactt
    62821 aactcagcgc tcgaaccaaa tgggatgttg aatttaccga caggaatcat tcgtcgccat
    62881 ccagattgcg tcattgtgat cactacaaat cgaaattacc aaggaaatcg cccattaaat
    62941 gaatctttaa gagatcggat gcaacatgcg gaaaaaatgg atcttcctgc cttagaagtc
    63001 atggcagaac gtgcgatggc gaaaactatg atccaaaaac aagaacttct cttaaagatg
    63061 gcagagatta tccgtttgtt ggatgaaact gcaaaagcta acgccatcaa aggtgtagca
    63121 ggaatgcgtt cttattttta ttgggcaaat acctataagc aaggacaaga tttactccag
    63181 tcgatttatc caaaagtttt gtataaatta tccactgatt cagatgaatt ggcaattctt
    63241 gaacaagctt tgtctgacag tggattgcta ctggaacttc aggacatatt gagaaaagaa
    63301 aaatggggtc atcctcaatc tgagcataca aaaggaagaa ccattagcgc cgaagaagca
    63361 aatgaacgaa acatcactga aaatgctgaa gaaattctgc gcttagatga aaagaaatcg
    63421 ttgtctgctg aagaaccaaa tgagggagaa gagaaacaag aagaaacaat agaaaaaaac
    63481 gagattcctc ctcaaatgca aaaagaacgc agtagcgata tcgaaggaaa aaatcaaaaa
    63541 gaacaacaaa caggagatga ttctcaagaa atgtctgctt ctgacatgga agcacatgat
    63601 aaggaaatca agaagcaact gaataaagaa gcgcgacaaa tcatgaagga aacgattcat
    63661 gaaaaagaag gtctcatcgt tcaccgacca gctttccaag gaaaaacgcc ggaagcacgt
    63721 gagctacaca tgcaagtaat gccaatagta gaatcactta gccggcaaat tttagagtta
    63781 ctggataatg aacaaacaga gacgtaccaa aaaggaaaat acgaaggtca gcggttcaat
    63841 gcaagcaaag tagcatatgg tgatttgaga aattttgaca aaaagaaccc gccacatgag
    63901 caaccttctt tagcggtagc tgtacggatc gatgaatctg ggtcaatgat acgggatgat
    63961 cggatcgaag cggcaaaaaa agcagcgatt gccattgccg aattttctaa gaaagtcgat
    64021 atcccattgt tgatttacgg agatacagca gaccgttcgt caagagaaaa gacttctttg
    64081 ttttcttata aagaatttga agacggattt caatggttaa atgaaaaatt agtcactatg
    64141 aaaccaaggc aaaacaatcg tgatggtgct gcacttcgtt tagcagcaga aaaattaaac
    64201 agacaatccg caacaaccaa actgttgctt aatattagtg acggtcagcc aaaagcgttg
    64261 ccggattata caggcaaaaa agcaaaagaa gatatccagt ctgttttgaa agaatatgaa
    64321 cgtcaaggaa tcctatttgt ctccgcagct attggccaag acaaagaaga aatcaagtcg
    64381 atctacggtg aaagtcgttt tgtagatatt actgacttga atgaatttcc caagcaaatg
    64441 attcaactga ttgcccgcta tctatgataa aataaagctc aacgaacgca cttagcagat
    64501 tcgttgagct tttcgtttct agttaccggt aacaaggata cttctaaaat agaatcggtt
    64561 ataatcaaga gggaccgtta aaggagtgaa acgatatgat acaagaacaa aaatggtgga
    64621 aagaagcagt aggctatcaa atctatcctg ctagtttcaa agatagcaat ggcgatggtc
    64681 aaggagacat caatgggatc cgtgaaaaac ttaattattt gaaagatctt ggtatcggct
    64741 ttatctggat caatccgatc tatcaatctc catttgtcga taatggttat gacgtaagtg
    64801 actatcaaga tatagaagag agattcggaa cgatggaaga ttttgattta ttgctaaaag
    64861 aagcacatga attagggatc aaagtgatta tggatcttgt catcaaccat agttcggatc
    64921 agcatcaatg gtttgaagaa tcgaaaaaat ctaaagaaag cccgtatcgc gattattaca
    64981 tttgggtcga cggggtagat ggcaaagaac cgaataattg gacatcgatt ttcggcggtt
    65041 ctgcttggga gtattctcat gaaactggac agtattatct gcatgttttt gcaaaagaac
    65101 aaccagactt aaattgggaa agcgagaaat tgaaagaaga actattcaat atgatccgat
    65161 ggtggttgga taaagggata gatggatttc gtttagatgc gatttctcat gtaaaaaaag
    65221 atgaatattc agtaaaagcc acagagaacc ctttctcacc ctttcaaaat gtctcaggaa
    65281 tcgaagaaca tttgaccgat ttgaaacatg tttttgaaga gtacgatatc atgactgtcg
    65341 gagaagcaag tggtgtcact gcagaagaag gaccacaatg ggtagggaaa gacggttatt
    65401 tcgatatgat attcgaattt gatcatatcc atatctggca gcaagaaaaa gaagggcaat
    65461 tagatgtcct taaattgaaa cttgcattaa gcgcttggca aacttcattg gatggtatcg
    65521 gatggaacgc tttatatatg gaaaaccatg atgttcctcg tgccgtttca gtgtttggag
    65581 atactcgtcc cgatttttgg gctatgtctg caaaagcaat cgctatgatg tatttctttt
    65641 tgcaaggtac gccatttatt tatcaaggac aggaaatcgg aatgacgaat atgccctttg
    65701 aatcgatcga tcaagttgat gcagtagatt cgaaaagact ttataaaagg ctgcttgcgg
    65761 aaggtaagac aagagaagaa gcattggata tcattcgtga aacaacgaga gacaacagca
    65821 ggacacccat gcaatggact tcggaacaat atgctggttt ttctacacat gaaccttggc
    65881 tgatcactaa tccaaatacc aaaacgatca atgtagaaca acaagaatat gaaccagagt
    65941 cggtcctgca attctataag aacatgatcc gcattagaca aaccaataaa ggtctgatat
    66001 acggttctta taaagaatat cttcatgaac atccacaact ttatgtctat gaaagatatt
    66061 tagaagatga acaatatctt attatggtaa acttaacaga atcacttgct gactatgaac
    66121 ttccaaaaga agcagatcag tcctggacat tactcttgtc aaattcctct agcggagaat
    66181 ttgaagctaa aggcatacta gcaccttatg aagctcggtt atataaaaca aacaagtaaa
    66241 acttcctcgt taaagttcat tagttggact ttgcgaggaa gtttttcaga ttgctctgtc
    66301 aattactcat ttcaataatt cgctctagga ccaccgatat atttaggacg agagcgaagg
    66361 gctgtcacgt gagcttgtcc aagggttttc aaacgaggtc tgacaggtac ttggacatgt
    66421 ttgatatgca taccgattga tgtgtcaccg atatcgatac cggcttgtcc aactacgtgt
    66481 tccacttcga caggatcatt gaactggtca aaagcagcaa cagaacaagc acctccagca
    66541 tgtagtgcag gaacaacaga gacaatttcg aaattttttg cttctgccac tgttcgttct
    66601 actactaatg ctcggttcaa atgttcacaa ccttggacag ctagcgcaat ttctttagga
    66661 tccaacaact cttttaacgt acggatgatc cattgaccaa cttccgcact ggaatttttt
    66721 ccaatatgtc ctccaacgac ttcgcttgtg ctgcatccta aaacaaaaag atcacctttt
    66781 ttcaagtgtg cctcagccaa tatgtcatta actatttcag tcaattggtc ttttaattct
    66841 ttttctgaca acttcatttt ctaaaacctt ctttttcaat taatattgct tccgtgcttg
    66901 aatacgttga agacttgcag ttataggaaa ggcaagtccc attccaatca gattttggaa
    66961 gatgtttcct ggtatagaag ccactccagc cggccaatca tataaaatcc aaccggcgat
    67021 accatagccg atgatcataa ctaagcttcc taataaaagc cctaatcccc acatacgagt
    67081 agaagatttg ctgaaagaac cagcaacata tccttgtaac ccgtggatca ctaatgaaaa
    67141 aactgcccac tgcggatatc ctgaaatcaa atcgatcaat agaccactgg aagcaccaac
    67201 aactaatcca cccgttggac cataaagaaa agcagcggta tagatcccgg cctcgcataa
    67261 tgtcacaaat ccattcgtag ccggtacagg gatcaaaatt gttaatgaaa gggcaactgt
    67321 caacgcaatc agcatagcat agatcgttaa ttgcttagtt ttcatttctc attcctcctt
    67381 tttgaatacc gccagaccag acacttccgg tatgatcgtt catcagcacg ccattagaaa
    67441 tacaatgggc aacaaatttt ttactttctg ctactgcttt ggtaaaatct gcccctaaca
    67501 caagattcgc tgttattgca gaagaaaagg cacaaccggc cccgtttact gttgtagtgt
    67561 cgattttttt atgagaaaat acctcataat gatcaattga atagagaaga tccacagctt
    67621 catctccagc catccttgtg ccgcctttga tcacaatgtt ttttgttcct gaagcagtga
    67681 gttctttagc gagatggatc atttctgtta acgaagaggg aagagcttga ccagaaagta
    67741 aagcagcttc tgccaaatta ggcgtaatca tcgtaacaag tggaaaaagt tcttcgatga
    67801 gctgcgaaat atacaattga ttgatcacag cttcagtttc tttgaatgcc aaaacagggt
    67861 ctaaaatgat tgggatattt tgttttgttt ttatgaagtc tcgaacaacc atgattgctt
    67921 cacttgaatt caataaccca attttgatac catctaatga agtcatttta tcaatcgaaa
    67981 ctaactgtcg tttaagcaat tccaaagata tagattccag cataaatcgc tcattaatat
    68041 ctagtgcccc tatagaagtc aaggcgctta gtccgaatag accaaaattc tcaaaagttt
    68101 ttaaatctgt ttggattcct ccaccagcaa atggatcaga accgccaatt gtcaagattc
    68161 ttttcataac attttcctcc atgaataaga gtataacgaa attttcaaaa aagaaaaaca
    68221 gccaattggc atatttttat ctcatccaat ttaaaaaaac aaaaagaaaa gaaagaccaa
    68281 aatggatctt tctttcctta aatagttagg acttttgtga ttttaattct ttctcgattt
    68341 gaagccgttg cactttcatt gtagctgttc gcgggattgc atcaaaatca cggatgatag
    68401 ggacgttcaa gcgaggaagt tcatggactt gctcccacca tcgattccaa tccatttctt
    68461 tatcaggagc taaagcaata atcggttgtg gagaatgatt tttatccctt acaatcacta
    68521 cttctgccaa aaaatctaac gaatctaata aaaagtcttc aatagctaat gtactattga
    68581 tcgtttcgat caaatccact tgccgatctt tcaaaaatag gtggccatgc ttatccataa
    68641 aaccgtaatc tccgctatcc caccaatcgc cataaacatt ttcctggaac cgtgcatctt
    68701 ccttgtaata agtcaaagcc cgtcctttag acagtaattg tatatgacca tctgtgtttt
    68761 caggtaggac attcccaact gaatctgtaa tccgagcgct tgtcatgtct tccagtccaa
    68821 cgcccatatc tcttgcatca cttgttttca aactctctag tgtatgagcc ttaagaatca
    68881 ttggtccgca ttcactttgg ccatatactt gaagaaatac cgcatcctgt gccatcgacg
    68941 ttcgcaaaaa tgtcttcatt gtctggttgt ttatagcatc aaaagtggag tgatagtatc
    69001 ggatacccga aaaagcttct ggatgctcct tagcagtaaa acgccactga acaaagttat
    69061 tcggatgggt ctctagtgca attggccggt gagcttccaa catatgaaca acttttgaac
    69121 tttgtgcgtt tgctaaaggc atcataggaa atcccatgga catcaaagaa gagataccaa
    69181 tattgaatct ggaatgcact ggagagatgt gaaaagcgat taatttcttt tccgaaattt
    69241 ttgtgaaaac tgttttttgc catttcgtcc gccatcccat agaatgagca gaatgacaaa
    69301 taagtttagg gataccagtt gtaccagaag tatgggtcat ataagaaatt tcatttttgt
    69361 ctaaaaatga ttgagaaaca gacaaagcag gttgttccat taaataaaga atagg
//
LOCUS       DACCKU010000003        65237 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.003, whole genome shotgun sequence.
ACCESSION   DACCKU010000003 DACCKU010000000
VERSION     DACCKU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 65237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65237
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.003"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            142..1224
                     /locus_tag="IVN13_000158"
     CDS             142..1224
                     /locus_tag="IVN13_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP7010682.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            1224..2027
                     /locus_tag="IVN13_000159"
     CDS             1224..2027
                     /locus_tag="IVN13_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7010683.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            2097..2837
                     /locus_tag="IVN13_000160"
     CDS             2097..2837
                     /locus_tag="IVN13_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7010684.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            2848..3852
                     /locus_tag="IVN13_000161"
     CDS             2848..3852
                     /locus_tag="IVN13_000161"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP7010685.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(3897..5741)
                     /locus_tag="IVN13_000162"
     CDS             complement(3897..5741)
                     /locus_tag="IVN13_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010686.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            5953..6471
                     /locus_tag="IVN13_000163"
     CDS             5953..6471
                     /locus_tag="IVN13_000163"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7010687.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            6599..7282
                     /locus_tag="IVN13_000164"
     CDS             6599..7282
                     /locus_tag="IVN13_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP7010688.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            7591..7664
                     /locus_tag="IVN13_000165"
     tRNA            7591..7664
                     /locus_tag="IVN13_000165"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7625..7627,aa:Arg,seq:tct)
     gene            7823..8182
                     /locus_tag="IVN13_000166"
     CDS             7823..8182
                     /locus_tag="IVN13_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP7010689.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            8305..9936
                     /locus_tag="IVN13_000167"
     CDS             8305..9936
                     /locus_tag="IVN13_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP7010690.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(9998..10639)
                     /locus_tag="IVN13_000168"
     CDS             complement(9998..10639)
                     /locus_tag="IVN13_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7010691.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            10921..11229
                     /gene="rplU"
                     /locus_tag="IVN13_000169"
     CDS             10921..11229
                     /gene="rplU"
                     /locus_tag="IVN13_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP7010692.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            11256..11600
                     /locus_tag="IVN13_000170"
     CDS             11256..11600
                     /locus_tag="IVN13_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP7010693.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            11613..11906
                     /gene="rpmA"
                     /locus_tag="IVN13_000171"
     CDS             11613..11906
                     /gene="rpmA"
                     /locus_tag="IVN13_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP7010694.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            12313..15147
                     /locus_tag="IVN13_000172"
     CDS             12313..15147
                     /locus_tag="IVN13_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7010695.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            15338..15829
                     /locus_tag="IVN13_000173"
     CDS             15338..15829
                     /locus_tag="IVN13_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP7010696.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            15932..16921
                     /gene="manX"
                     /locus_tag="IVN13_000174"
     CDS             15932..16921
                     /gene="manX"
                     /locus_tag="IVN13_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP7010697.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            16954..17757
                     /locus_tag="IVN13_000175"
     CDS             16954..17757
                     /locus_tag="IVN13_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP7010698.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            17778..18692
                     /locus_tag="IVN13_000176"
     CDS             17778..18692
                     /locus_tag="IVN13_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP7010699.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            18775..19149
                     /locus_tag="IVN13_000177"
     CDS             18775..19149
                     /locus_tag="IVN13_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP7010700.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            19288..19533
                     /locus_tag="IVN13_000178"
     CDS             19288..19533
                     /locus_tag="IVN13_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7010701.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            19705..20769
                     /locus_tag="IVN13_000179"
     CDS             19705..20769
                     /locus_tag="IVN13_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7010702.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            21015..21440
                     /locus_tag="IVN13_000180"
     CDS             21015..21440
                     /locus_tag="IVN13_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7010703.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            21433..21882
                     /gene="nusB"
                     /locus_tag="IVN13_000181"
     CDS             21433..21882
                     /gene="nusB"
                     /locus_tag="IVN13_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP7010704.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            21979..22833
                     /locus_tag="IVN13_000182"
     CDS             21979..22833
                     /locus_tag="IVN13_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP7010705.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            22834..24171
                     /locus_tag="IVN13_000183"
     CDS             22834..24171
                     /locus_tag="IVN13_000183"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP7010706.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            24176..24400
                     /locus_tag="IVN13_000184"
     CDS             24176..24400
                     /locus_tag="IVN13_000184"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP7010707.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            24402..25292
                     /locus_tag="IVN13_000185"
     CDS             24402..25292
                     /locus_tag="IVN13_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7010708.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            25301..26119
                     /locus_tag="IVN13_000186"
     CDS             25301..26119
                     /locus_tag="IVN13_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP7010709.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            26226..26675
                     /locus_tag="IVN13_000187"
     CDS             26226..26675
                     /locus_tag="IVN13_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7010710.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            26693..28375
                     /gene="recN"
                     /locus_tag="IVN13_000188"
     CDS             26693..28375
                     /gene="recN"
                     /locus_tag="IVN13_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP7010711.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(28430..28546)
                     /locus_tag="IVN13_000189"
     CDS             complement(28430..28546)
                     /locus_tag="IVN13_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP7010712.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(28619..29575)
                     /locus_tag="IVN13_000190"
     CDS             complement(28619..29575)
                     /locus_tag="IVN13_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7010713.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            29746..30114
                     /locus_tag="IVN13_000191"
     CDS             29746..30114
                     /locus_tag="IVN13_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP7010714.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            30284..30715
                     /gene="mraZ"
                     /locus_tag="IVN13_000192"
     CDS             30284..30715
                     /gene="mraZ"
                     /locus_tag="IVN13_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP7010715.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            30736..31695
                     /gene="rsmH"
                     /locus_tag="IVN13_000193"
     CDS             30736..31695
                     /gene="rsmH"
                     /locus_tag="IVN13_000193"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP7010716.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            31700..32101
                     /gene="ftsL"
                     /locus_tag="IVN13_000194"
     CDS             31700..32101
                     /gene="ftsL"
                     /locus_tag="IVN13_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP7010717.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            32102..34294
                     /locus_tag="IVN13_000195"
     CDS             32102..34294
                     /locus_tag="IVN13_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7010718.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYPYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            34320..35282
                     /locus_tag="IVN13_000196"
     CDS             34320..35282
                     /locus_tag="IVN13_000196"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP7010719.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            35296..36657
                     /locus_tag="IVN13_000197"
     CDS             35296..36657
                     /locus_tag="IVN13_000197"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP7010720.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            36683..37771
                     /gene="murG"
                     /locus_tag="IVN13_000198"
     CDS             36683..37771
                     /gene="murG"
                     /locus_tag="IVN13_000198"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7010721.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            37786..39006
                     /locus_tag="IVN13_000199"
     CDS             37786..39006
                     /locus_tag="IVN13_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP7010722.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            39162..40487
                     /gene="ftsA"
                     /locus_tag="IVN13_000200"
     CDS             39162..40487
                     /gene="ftsA"
                     /locus_tag="IVN13_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP7010723.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            40510..41751
                     /gene="ftsZ"
                     /locus_tag="IVN13_000201"
     CDS             40510..41751
                     /gene="ftsZ"
                     /locus_tag="IVN13_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP7010724.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            41768..42445
                     /locus_tag="IVN13_000202"
     CDS             41768..42445
                     /locus_tag="IVN13_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP7010725.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            42459..43058
                     /locus_tag="IVN13_000203"
     CDS             42459..43058
                     /locus_tag="IVN13_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP7010726.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            43073..43360
                     /locus_tag="IVN13_000204"
     CDS             43073..43360
                     /locus_tag="IVN13_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP7010727.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            43409..44191
                     /locus_tag="IVN13_000205"
     CDS             43409..44191
                     /locus_tag="IVN13_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP7010728.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            44212..44919
                     /locus_tag="IVN13_000206"
     CDS             44212..44919
                     /locus_tag="IVN13_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP7010729.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            45181..47979
                     /gene="ileS"
                     /locus_tag="IVN13_000207"
     CDS             45181..47979
                     /gene="ileS"
                     /locus_tag="IVN13_000207"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP7010730.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(48028..49554)
                     /locus_tag="IVN13_000208"
     CDS             complement(48028..49554)
                     /locus_tag="IVN13_000208"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP7010731.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(49569..50216)
                     /locus_tag="IVN13_000209"
     CDS             complement(49569..50216)
                     /locus_tag="IVN13_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP7010732.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(50400..50729)
                     /locus_tag="IVN13_000210"
     CDS             complement(50400..50729)
                     /locus_tag="IVN13_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP7010733.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            50906..51634
                     /gene="sfsA"
                     /locus_tag="IVN13_000211"
     CDS             50906..51634
                     /gene="sfsA"
                     /locus_tag="IVN13_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP7010734.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            51650..52663
                     /locus_tag="IVN13_000212"
     CDS             51650..52663
                     /locus_tag="IVN13_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7010735.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            52663..53940
                     /locus_tag="IVN13_000213"
     CDS             52663..53940
                     /locus_tag="IVN13_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7010736.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            54003..56705
                     /locus_tag="IVN13_000214"
     CDS             54003..56705
                     /locus_tag="IVN13_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7010737.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            56857..57174
                     /locus_tag="IVN13_000215"
     CDS             56857..57174
                     /locus_tag="IVN13_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7010738.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            57204..57524
                     /locus_tag="IVN13_000216"
     CDS             57204..57524
                     /locus_tag="IVN13_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7010739.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            57632..59092
                     /locus_tag="IVN13_000217"
     CDS             57632..59092
                     /locus_tag="IVN13_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7010740.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            59160..59381
                     /locus_tag="IVN13_000218"
     CDS             59160..59381
                     /locus_tag="IVN13_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010741.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            59412..59594
                     /locus_tag="IVN13_000219"
     CDS             59412..59594
                     /locus_tag="IVN13_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP7010742.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            59594..60007
                     /locus_tag="IVN13_000220"
     CDS             59594..60007
                     /locus_tag="IVN13_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP7010743.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            60130..61311
                     /locus_tag="IVN13_000221"
     CDS             60130..61311
                     /locus_tag="IVN13_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7010744.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            61700..61773
                     /locus_tag="IVN13_000222"
     tRNA            61700..61773
                     /locus_tag="IVN13_000222"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:61734..61736,aa:Arg,seq:cct)
     gene            complement(61842..62981)
                     /locus_tag="IVN13_000223"
     CDS             complement(61842..62981)
                     /locus_tag="IVN13_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7010745.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(63280..63915)
                     /locus_tag="IVN13_000224"
     CDS             complement(63280..63915)
                     /locus_tag="IVN13_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010746.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(64028..64663)
                     /locus_tag="IVN13_000225"
     CDS             complement(64028..64663)
                     /locus_tag="IVN13_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP7010747.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(64697..65158)
                     /locus_tag="IVN13_000226"
     CDS             complement(64697..65158)
                     /locus_tag="IVN13_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP7010748.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        21386 a        10975 c        13743 g        19133 t
ORIGIN      
        1 aagaaaacag gcgagtgttg ctcgcttgtt ttctttattt ttgaatattc tgaataaaag
       61 ataaaaatac caagtttgcc tgaacaaaaa tcataagcaa tggtataatc acaatatgaa
      121 tatagaaatg ggggaaaaca tttggataca aaagaagaac agctacttat tgatttaact
      181 tctgcaaaag gagtacctgg aaatgaagaa gaggtaagag aagtattcag agaatatgcc
      241 aaaccattcg ctgatgatat tttttatgac ggtttaggca gtgtgattgc caaacatggt
      301 gctggcggag gccccaaggt ctttatttct ggacatatgg acgaagttgg ttttatggta
      361 acaaaaataa ccgaaaaagg attcttggaa ttccaaactc ttggaggttg gtgggggcaa
      421 gtcatgcttg ctcagcaagt tgaaatcaaa acacgtgaag ggaaaatcgt ccacggtgtg
      481 attggttcca aaccaccaca tgttttaaca cctcaagtaa gaaataaacc ttatgaaata
      541 aaggatatgt tcattgatat tggcgcatca agccaagaag aagccaaaga gtggggcata
      601 cgtcctggag atatggtcac tccatatatt gaatacaaac gaatgaatgg cagtaaatat
      661 ttactggcaa aagcatggga taatcgtatc gggacagctg tttctctgcg agtattagag
      721 aatctatcta aagaagcaca cccaaatgtt ttattcgcag gaagcgatgt gcaagaagaa
      781 gtaggcttgc gaggtgctcg aacaagtaca caccttgtaa atcccgatat tgcttttgct
      841 ttagatactg gtacagcagg agacactcca ggaatgacac caaaagaagc agattcgatt
      901 ttaggaaaag gcccacagat tttgattttt gatgcatcta tgatcccgca taaaaaatta
      961 ttgaattttg ttattgatgt agcagaggaa atggatattc cattccaata tacagtgatt
     1021 tctggcggag gaactgatgc aggacaaatg catttgacaa gagatggtgt tccgtctgta
     1081 gccattaccg ttcctgttcg ttatctgcat tcccatacat cagtgattca tgaagatgat
     1141 tatttgaata cagtgaaact agtcacagaa ttagtgaaac ggcttgatag tgaaacagtt
     1201 gaaaaaattc ggagttatga ataatgaata atagaatacc tatttctgaa tatcggaaaa
     1261 aagcacgagt ttctttggaa aatcaatggg gaatcaatac ttggcttgtt ttcttatcta
     1321 taatatttac gagcgtcatc cagaactttt ttgacggttt cagtcctttt ccggaagaaa
     1381 gcatgcagca gcacttattg agttttctag tcgataattt acttttattc gcattcaact
     1441 atgcattgtt ttacattgcg ctagtagttg tacggggtgg cagaccaaaa agtaaccttt
     1501 tattttcagt tttccaaaga aagtattttg gttcgatact tggcatcaat ttgatcaatt
     1561 cagtcatcaa ctggctactc aacagtttaa tctttctgcc catgttttta atcagcggag
     1621 tgagtactta tatgcagctg cttttcagtt caaatgcggt acctacgggt gtcatgccag
     1681 aagattttct tgatatcagc tttgttttat cgatgatagg gatgattttt gtttcagcac
     1741 tcatcatgag tattgttggc ggtttattcc aatttgctgc atggacaaag ttcgactatc
     1801 ctgatttgtc ggttattcaa tgtttgaaat atggctggtt tttattgaaa gatagaattg
     1861 gtacgtatat tttgttgcag ctgtctttta tcggctggta tttgctagga gcaatcgctt
     1921 tgttcatcgg tcttttgtgg gtcattgttt atgtgaatgt gtcagttgca gaattttatg
     1981 aacaagcacg gattgaaaaa ggaggtccag acgcctattt tacttagcat gtaagcagtt
     2041 tattcatcaa aaaaataact tttgtggtag gataaatctt tgagggggaa tgtacgatga
     2101 aattaactgt tttaggatgc ttaggagcgt atccttataa aaatcaaggg acaacaggtt
     2161 atttgcttca atctgacggg ttcaatttgt tgatcgatgc aggtagtgct acgctgatca
     2221 aattacaaga acaccttgat ccacttgatc tagatgctgt gatccttagc cattatcatc
     2281 atgatcatat tgctgacctt ggtgtattac agtattactg gcaacttcat cctaagagaa
     2341 atccggagac attgctttca atttatggac acacaaaaga ttccattcac tttgaagaat
     2401 tgacgttgga aaatgtctca aaaggacatc cttattttga agctgaagaa ttaaaaattg
     2461 gtccttttct gattactttt atggaaacaa tccatccagt agtatgttac gccatgcgta
     2521 tcgtagaaga aacaacgggg aaagtattcg tatttacagg tgattcaggt tatttggaaa
     2581 gtttcgctga ttttgcgaaa gatgctgatt tgtttctagc agatacctat ctttttgaag
     2641 gaaatgaaca tcatcatgcc catttcactt caaaagaagc tggagaactt gcgaaatcag
     2701 ctaacgtaaa gaaacttgta cttacacacc tacctcagtt tggtgattta gaacaattga
     2761 aaaatgaagc gcaaaaggca gcaggcaaag ggattgatat agaacttgct gcagttgaca
     2821 aagtgtttaa catttaggag gaagaacatg atttttgtac caaatgaaaa ccacgaccca
     2881 agaatcaatt tagcaattga acaatatttg ctgcaagaaa tgcctttaga tgaacctatt
     2941 ttgctctttt atatcaatga accttcaatc atcatcggca gaaaccaaaa tacgcttgaa
     3001 gaaatcaatc gagattatgt ggaagaaaat ggcattcatg ttgtccgtcg cttaagtgga
     3061 ggcggagctg tctaccatga ttttggaaac ctgaacttta gttttatcat gcctgatgat
     3121 ggggattcat tccgtgactt tgcgaaagta acacagccaa tcatccaagc acttcatgaa
     3181 ttgggtgttg ctggggcgga attgaaaggc cgaaatgatc tagtcattga tggcatgaaa
     3241 ttttctggca acgcgatgta tgctaccaac ggtcggatgt ttgctcatgg aacaattatg
     3301 ttcgactctg atatcaatga agtagtcaat gcattaaaag taaaaaaaga caaaatcgaa
     3361 tcaaaaggaa tcaaatcgat ccgttctcgt gtaacgaata tcaaaccatt tcttccagaa
     3421 gaaaagcaaa aaatgacaac agaagaattt cgtcaagaaa tcttgttgaa aattttcggc
     3481 gtagagtcag tagaagaagt aaaaacgtac gaattgactg atgaagattg gaaaaaaatc
     3541 aatcgaatct ctgaggaata ttatcgaaat tgggattgga attacgggaa gtctccagat
     3601 ttcaatttct ctcgccaaaa acgtttttct atcggttcga ttgaagtcca tttgaatgtc
     3661 tcagaaggaa tgatcaaaga agcaaaaatc tttggcgatt tcttcggact aggagatatc
     3721 tctgacgtgg aaaaaaaatt ggttggacag aaatatgata aagcatctct atcaaaaata
     3781 gtcagccaaa ttgatgtgaa gaaatatttt ggtgcgatag aaccagaaga attgcttacc
     3841 ttactttatt gaaaataaaa aatagcggaa tccacatata tggattccgc tattttttat
     3901 acaggattcg ggtaatttcg agagagttga acagtgatcc gatcaatgaa agtatctttt
     3961 tcctgcgtta gtgttcctgt gtaacgatcc gctacgtctt tcatcatcat taagtcaaaa
     4021 cgttcctgat catctaattc ttctgtgatg cctcgactaa ataggtttgt atcgtaatgt
     4081 gcactatgtt cgcaacaaat gacgaaatgt tcctcttgtt cgtgtatcga caaataaatt
     4141 tttcgataca gtggattttc gatggcatga gtttctctaa aggaatgctc taaagcgtgg
     4201 atcaaaagtt gagccagatc gtcatcgttg ataggaagtt cttcgggaag atctgcttgg
     4261 aaggttatct gaatattccg tttggcagct aacttctggt aacgcgctaa gataagattt
     4321 gtcacctggt ggttgcaaaa agaacacagc atttcgaaat tttttgcttc ttctaatatc
     4381 ttttccacat agtttttagc ttttgtttgg tccgtatgca ttaaatcttg cagattctcc
     4441 atctcttgga caaattccag gcgaagtgta tttacttctt ggatatgctg tcgaagctgc
     4501 tcataatgtt gctccaacaa atcatttttg atttttaaga aatctacttg tctccgatct
     4561 ttgtcggtat ttaagatgtg atcgattaga ttgtagctga taataacgat tagaatgata
     4621 atatataaaa tcgtagacca attgatcgtt gtttgataga tacctaatgc cgtttggatg
     4681 taaatcaagc aatggataat ggctaccaag acgatccaag gtgtacaagc tacataaaaa
     4741 cggttattct tatatgcttc gccaatgcat gcaagtgaag tgactaaagt gcttataaca
     4801 ctagcaatat tagcaatcgg cattaattca ggaagttctg caggagtaaa agttgcataa
     4861 agcagaacgc cgataaaaat caaaagttgg aacgtacaaa aaattccgta ccatttttta
     4921 tagtaaaaca ttcggctcca atagtagcag atgatgaaag ctgacgttaa gataataaat
     4981 aaatgagata aaaaagaatg aacgaatggt tcaaaaagaa taccacttaa aatatcttct
     5041 gatacagctt caaatcctaa tgccaaagca aagcaactta agagaataag tgagtattca
     5101 agacgttttt gtttatacat caagtagatc gtatagaaaa aggtccctat agcgataaaa
     5161 acagcgatta tcatcgtcaa tacttgcggc actgattgtg aataaataaa actaatcaat
     5221 ggtccagttg ttcctagata aactttcggt ggcagtccag catagttctg ataaggagat
     5281 gagactgtaa gggtcaattt ttttccgata taatttgctg gtaaatctat cacagccaat
     5341 gagagtccgg gattgctttc ttcctgataa tctttccgtt ggtataaaat cttttcgcct
     5401 agatatacag taatccattg atggtttcct ttgattagta aatttgcttc gtccaacggt
     5461 ttttcaagtg tcgtttccat aatgaatgtt tcgtctttat ccacggtagg aatcgtacga
     5521 gtgtacttgc tgtcaaactt aaaatcagga tcatcttttg tatagaaaac ccacgaatct
     5581 tttatttctt tcatttgatt gctgtccgca ggagaaaaaa taaaataaaa gaaataagca
     5641 attcctgtta aaaataacaa tgataatata atgattatgc tccactttat acgtaaataa
     5701 aaagattctt ttttattata tatgttatta aaaaaagaca taaaggtatt cctttctaaa
     5761 acttgtaatg ttagggtaaa cgttttttaa actaacgtca attatttata ataaaaaatg
     5821 agataaagat gaggaaatag aattagaaac atataattat taaagggaaa catttttaca
     5881 gctataattt gaaaaaaatt tatcgttatt atatgattaa gaaggattga ataaagaaag
     5941 aagttgaaaa cgatacctga ttacaaaaaa ataactttta taagttctat aacgttgatt
     6001 atatttttaa ttcttattct tctcgtcgtc ttggttcccc gaatttttgg tttgactcct
     6061 tatattattg aggacaaaga aatgagtcca tattatgcac aaggaaatct gatatatgta
     6121 cgaccgaaaa atacaaatga gattttagtc ggagatatta ttacatatta tgaaaattca
     6181 ggaaaaaaaa tctcgacgcg tcgcgtaata gccgttgaaa acaaccacga agatttttat
     6241 acaaaagctg atacaaagac ttatattgag cctggagtag taaaaaagag aaatattatc
     6301 ggtagtccca tctttcagat tccatatatt ggactagtcc tttcaaaaac ggcatttgca
     6361 tggattcagg ggttattctt tacgattgct ttttgcctaa caggaatcac agcatggaat
     6421 acctttgtag agttgaaaaa gaagcggaga cgaacacaga aatttttgta agaatgttaa
     6481 aaacataaat gtattccaga gatccaacga ctcttctgaa catgaaacac gagaaaacac
     6541 tcatgcagct tgtagattta gtcagttgtt cgtataatgt gtgaagagga ggagtagtat
     6601 gcgcaatgaa atgatgcatc gtcctgtagt caaagaagaa ctattggatt ttatgcgtac
     6661 ccagcaaaaa aaattaccgg gtgagctagg caaactggaa gaagaagctc atgtagcaga
     6721 agtaccagtt attccccatg aaacagttgt atttttgaaa tttttacttg gacagcttca
     6781 gcctgaacga atcttggaga tcggagcagc tatcggtttt tcatcaagtg tcatggccac
     6841 gacaatcgga gaaaacgctc atgttacgac aattgatcgt ttcgatgtca tgatcgagaa
     6901 agctaaaaaa aactatgaac gtctaggact gacagataaa gtgactttgc ttgaaggtca
     6961 agcagcagat atcttgccgg agttgtctgg tccgtatgac tttattttta tggatagtgc
     7021 aaaatcaaaa tatattgaat ttttacctga atgcttacgt ttgttaagaa aaggtggcgt
     7081 tttgatggta gatgacatct tccaaggagg aacgatcttg ctcccagatg aagagattcc
     7141 ccgtggcaaa cgagctatcc atcgtaaatt aaatgaattt cttcgtgttg tgatggatca
     7201 tcccgatttg acatcgacaa tcttgccatt aggtgatggt gtgatcttaa tgacaaagga
     7261 atcagaaaca atcgagttgt gatacatgat tatggcagcc tgtagataat cgattgaatc
     7321 gagggctcaa ttcgaacttg atgtaaaagt ttctatattt gtaagtcaat taaaggatac
     7381 aggaaatcta aataaacagg agtagcatga agtcaatgta gctcctgttt tcaattgata
     7441 accttagaac agctgaagga gaatgtctcg ttttaaaaga ttgatttagt agttgggcca
     7501 aaataattca ttatttgcta attaaataac tatttcgtat tgacagaagc aatttaatta
     7561 tctataatta ttgatgttcc gctgattctt gtcatagtag ctcagctgga tagagcatcc
     7621 gccttctaag cggacggtcg ggggttcgaa tccctcctgt gacgctaaaa aaccgttaga
     7681 aactgaggtt tctaacggtt tttctttgtt ttttgtgcca atattgtttg ttttcaaagg
     7741 caaataaaat aaaaaaacat ccttatcgag tacaattata aggagcaaaa aaataaaact
     7801 catcagaaaa gaggaagaag atatgacgat actatctaga attttagtga ctctagttgc
     7861 aattgaattt ttttatatca tgtatatcga aacggtgaga actgattctg acacgacaag
     7921 tagggttttt aaaatgagta aagaagagtt aagcagaaaa tctgttcaaa cattgtttaa
     7981 gaaccaagga gtgtacaatg gactattagg agtgggacta ctttatggag cttatctttc
     8041 ttccgcatct aaagaaatca caagtatgct attaatatct attttttttg ttgcattata
     8101 tggcagtttg actagcgatc gttggatttt actcaaacaa ggtgggctag ctctgttggc
     8161 attgctttct attttcttat aaatatagaa aaaataagaa tttatctaac aggaaacttt
     8221 gtgaaaaata ttactttatc tcatgaaata tgatattcta atgaatggta ttttagaaaa
     8281 tttcatgaaa aaggagttag ataggtgttc aaaaattatg tttcctcact aaaaaaagaa
     8341 ttttcaggtt atgatctttc aagatttcag aaagatcttt tggctgggat cacagtagct
     8401 gctgtagcat tgcctttagc cttggctttt ggggtatcaa gcggagctga tgcggccgca
     8461 ggtttgatta cggctatcat tgccggtatc gttattggcg gtttatcagg cggtttttat
     8521 caaatatcag gtccgacagg tgcgatggca gctatcttga tgtctattgc ggcaacaaag
     8581 ggaatgcaag gtgtattgct tgctactttt ttagcgggtc tatttttact tgcagcagga
     8641 cttttccgat taggaacact gacttctttt atcccagcac ccgtaattac ggggtttact
     8701 tcaggtattg ctatcattat tgcattaggt cagatcgata atttatttgg tgttcactcg
     8761 gaaggtacaa acgttattga aaaactaagt agttatcaaa cgataggatt tccgataaac
     8821 acagcctctt tgatcatggg agggctagtc attttaggga tgtttatcta tccaaaaaaa
     8881 tgggcgaaac gaatgcctag ttcgctgctg gctatcattt tggcaacagc agttatgcta
     8941 ttgttccatt taccagtagc aactgtgggt aagattccac aaacattgat cagtagtaat
     9001 cgtttgaata tgggagattt tagcttttct gccttgcaac aagtggcagt tccagctatt
     9061 agcattgctt tattaggaat gattgaaagc ttgctatgtg gtgcttcggc tggccgaatg
     9121 gcaaataagc ccttagatag caatcaagaa ttaatggcgc aaggaattgg aaatctgttg
     9181 cttcctttct ttggcgggat tcctgctact gcggccatcg cccgtacaag cgtggccatc
     9241 aaatctggtg cacaaactag attagctggt atgatacatg cagttgtctt actgctctcc
     9301 atgcttgttt ttgcgccagt gatgtcacag attccaatgc cagcacttgc aggtgttttg
     9361 attgtgacgg cttggcgtat gaatgaatgg gaaacgatca aggagctatt tactaataaa
     9421 tattggtcag cagttctatt gtttatcctt acgatgattt gtacagttat ctttgattta
     9481 agtattgcta tcgtgatcgg ggtgatcggc ggctgtgtat tttttgttgt caaaagcgct
     9541 gctatcacga tttcattaga aacgatcgat tggcaaagaa tggggctgcc agaaacggaa
     9601 aagctgaata actggacagt agtgtatatt agtggtccac tgttttttat gtcttcagaa
     9661 aaactcaaaa ataaattgga agaactaaat catcacgatg gtgtcatttt ctccatgcga
     9721 ggcgtaccaa gtattgattt gacggcacgt gcagtttttg atgaatttca agaaaaagca
     9781 gagcaaagag gacaaacgat tatctatact tccttgcagc cagaagtaga aaaacaactt
     9841 tctcacttgt gggaacatac attcacgcat caacatcaaa cagttgctca tgcgttgaca
     9901 gctttacatg cgaaatatac tgcaattagc cattaaaaca atgaaaaaga acagacaaga
     9961 ctgtaaactg aagattttat ggtcttgtct gttcttttta atattgctgt aaaagtgaag
    10021 ctagtacttg ccagtcttga tggataacag gtacaaggga gcgattgtag aaaatagctg
    10081 ctggatgaaa aagtggaaga attcgatagt ttttttctga ccattcgtag gcatcctttt
    10141 gttctgttaa ttgtaagaca ggccctgtat agagttgacc atgatttttg gtaaccgtat
    10201 attctttgcc aagtaaacgt tgaagaccaa tgtttccaag tgtcgcaatt aattttggct
    10261 ggaccttcat cagttcgtaa tccaatattg gtgcatgcgc aaatacttcg gaacgagtag
    10321 gtgtccgatt tggataaaca gtttctgact gttgtgtccg tttattgatc cgatgagtca
    10381 cgcgatacgg gcggctcctt acagcactgg tgatatacac ctcttctcgt gttaagccag
    10441 ttgaagaaag tgcctgcatc aattcttttc cagcttgacc gctaaaagga acatgcgtat
    10501 caatctctgt tttccctggg gcttctccca cgagcatcag tttaggatga agaggtcctt
    10561 gcccagcgat aaatccttct acaggacggc cgttcattct taaagaagct tcttgtatca
    10621 gttcttttgg gtattccatt tatgctcctc ctttttcttt tattataaga ataaaagaaa
    10681 cacaatgcga ctattttatt tggtagaact gtgaggatag aaaagcagaa gtattgcttg
    10741 aatttttgtt ttactttttg attttaaatt tagttgacta acggttgctg ttctggtaag
    10801 ctagtactgt tgtaattgtg gactccacag ctacaaccgc tcagaacaag taataagcac
    10861 agcatatgct gtcacttcgg atggcgagtc taagtatatc agaaggaggt gctttatagc
    10921 atgtacgcaa ttattaaaac tggtggtaaa caagtaaaag ttgaagtggg acaagcaatc
    10981 tatgttgaaa aacttgacgt tgaagcagga gaaaaagttg tttttgacga agttatctta
    11041 gtaggcggag aatctacaaa agtaggcgct ccaactgttg caggtgcaac tgttgaaggt
    11101 actgttgaaa aacacggcaa acaaaagaaa gtcgttactt tcaaatacaa acctaaaaaa
    11161 cattcacacc gtaaacaagg tcatcgtcaa ccatatacaa aagtggttat cgacgcgatc
    11221 aacgcttaat agcgtttgag aaagaaggcc tatatatgat caaaggaaca tttaagcgaa
    11281 atgacgcagg tcagatcgtt tcattcactt tgaccgggca tgctgatgct ggtccttatg
    11341 gaagtgatat cgtttgtgcg ggtgtttctg cgttagcgat cagcactgtc aacgggattg
    11401 cctcattagc cggttttgag ccgattgttg aaatgaatga agaagaaggc ggttatcttt
    11461 ataccgaagt cacttcagga atgacacaag aacaaaacaa tattgctgag attctattag
    11521 aaaatctttt attaggatta caatcgatag aagctgaaaa ctcagaatat atccaaatta
    11581 aaacaattaa tgataaatag gaggtgcaga ccatgttgtt atctatgaat ctacaattat
    11641 ttgcccacaa aaaaggtggc ggttctacat ctaacggccg tgactcagaa tcaaaacgtc
    11701 taggtgctaa acgtgctgat ggacaaacag ttacaggtgg atcaattctt taccgtcaac
    11761 gcggtacaaa aatctatcca ggtgtaaacg tgggtatcgg tggagacgac actttatttg
    11821 caaaagttga tggcgtagta cgtttcgaac gtaaaggccg taacaaaaaa caagtgtctg
    11881 tttatccagt agctcaagaa gcttaattga atcttatgat taaagaggtt gtgacaagac
    11941 ttttgtcaca gccttttttg tttataaaaa taaaagttgt agtggaggct acgaagttta
    12001 ctttgtcttt ttacacttaa tatttttttg ccaatttgga atcaggatgt tttgataact
    12061 atcgttttca attgatggaa gctatattgt ttgctatcag tgaaataatt atatggtaaa
    12121 cagtgttctt tttcttgttt ccattataaa aatcgtatcg ggatttttcg ttcttttaaa
    12181 ttgaacattt ctatctatcc gcgtgttatc aacaaaacaa aatcaggtaa atagttgacg
    12241 aaacagaaat aaatacatag tattatgttt tgaagcctaa gtacaatagt ctatagaaaa
    12301 ggagcgatac cattggcaaa acgcattgac cgaatttata tgtatgtcaa agaaaaaacc
    12361 gctcatttaa caaaggcaga atatgatcaa ggagtaacaa cacaggaaat tgctgaagtg
    12421 ttagggattc agcgaacaaa tagcagtaaa gatctgaatc agctagtgcg cgaaggaaaa
    12481 ctgataaaga cagacggacg accagtacgt tacatatatc aaacactcgt tacgcacgta
    12541 aagccagtta caaaacatgt agtcagttac aaagaaacgc cggtctctga agaaaaaaat
    12601 acactccaaa tagatactaa ggatatcttt gcaaaaatca ttggagctaa cggcagcatg
    12661 aaaaattcag tggaacaggc aaaagctgcg attttgtatc cgccaagagg attaaattgt
    12721 ttgattacag gacctaccgg atcaggaaag acttattttg cacacgcgat gtatcatttt
    12781 gcaaaagcgc atcatgtaat agatgaggaa acggaactga ttgttttcaa ttgtgctgat
    12841 tatgccaata accctgagct tttgatgagt catttatttg ggtacgcaaa aggtgctttt
    12901 acaggcgcag atgaagaaaa aatggggatt atcgatcaag cggatggcgg tatgctgttt
    12961 ttggatgaga ttcaccggtt gccgcctgaa ggtcaagaaa tgatctttta ctttatggat
    13021 catggaactt acagtcgtct tggggaaaca acgaaatccc atgaagcaaa tgtacggatc
    13081 gtaggtgcga ccacagaaga cccaggttct tctctattag aaacttttgt tcggcgtatc
    13141 ccaatcaata tcaaacttcc ggcttttgaa aaacgtcctg caaatgagaa agtcgattta
    13201 gtaaaaatca tgattgccca cgaagcaaac cgcacgcagc gaaagatttc tttgacagaa
    13261 gatgtagcaa aagcattgat cggcagtgtg acctatggaa atataggaca attgaagtca
    13321 aatatccagt tagtatgtgc acgaggattt ctaaatcata tgcaatcacc cgaaatctcg
    13381 atcacaatcg atgatttgac agaagggatt cgtagcggat tgattcaatt agcaagcaac
    13441 cgcagtgcaa tggcagagct ttctaaaata ttggagccaa agataactgt agcaccaaat
    13501 gatacgatca tgaaaatcca atcggactcc tatgagttac cttataatct atatgatatc
    13561 atcggagata aagcagcttt actgaagtcg gatggtttgg atcaagaagc aatcaatcat
    13621 ttcatttcta cagatatcaa catccatctg aagtctttct ataaagacca tggtttttct
    13681 tttaatgcag acagcaaatt agcagaattt gtagatccga aaatcataga agtgaccaat
    13741 cagatttatg taatggtcaa aagatcttta ccatatgagt ttcagcaaaa ctttatttat
    13801 gctatgagct tgcatatcag ttcttttttg aaaaaaatca acttaggaga agaacggcat
    13861 acgaatgata acatccgtga gatggctttt gattttccag atgaatatgc tgttgcgaaa
    13921 gaagtgcgta gttatatcga aacgtatttt caagtggaga ttccagaaag tgaagactat
    13981 tacttgaccg ttctactcgt ctcgttacgg gcaaatcaag caagcggcag aatcggtgta
    14041 gtcgttgcag ctcacggaaa cagcacagca agcagtatgg tccaagttgt ccaacagttg
    14101 ttagatgcag agaacgtacg agcagtagac atgcctttgg atatggatcc gaaaacagca
    14161 ttggaccgaa ttgaacgcaa tgtacaagaa gtagatgaag gaagcggggt catactctta
    14221 gtagatatgg ggtcccttgc gtcgtttaat tcgcaaattc aaagagacac tggaattcca
    14281 gtacgtactg ttgatatggt aacaacttct ctagttttgg aaactgtacg aaaggtctca
    14341 gtattaggga cagatctaga tatgttgcat gaatcattga aaaattttcg aggttacgca
    14401 gaaatatctt ctgtagaatc tgttcaggaa gcaaaaccta ttcataagaa aaaagctgtt
    14461 ttggctatct gcgcatctgg cgaaggtaca gcacaaagaa tcaaagaatt gatcgaacga
    14521 gcggtaaaca agcgtcaaga aacagaatta gaagttctag ctttgtcgat tgtggaagtg
    14581 aaagaagaac tgccagttat ccaaaaagaa tatcagatca tagctactac tgggattact
    14641 gatttgaata tccaagcccc atttattcct ttagaacgtt ttattgatca aaatatcgaa
    14701 gtcattttgg atcagttgct gatggaatca gaattggagg aaactgaatt catctcccta
    14761 gatgaagagt cagctaaaaa tacgtgtgtt gagtttatct ctgataactt catctttatt
    14821 aatggtagta agttaatcga tccaatgtgg caattttcta cacaaatctc tcagacgacg
    14881 ggaatcggcg atgaagaata tggattcaaa atcaacctag tgatgcacac agcaggaatg
    14941 atcgaacgta ttattcggaa tgaaccattg actgtggaag aaaacgaatt gacaaataca
    15001 acgaatgatc ctctttatag ccaattggca gcctcagttg ttctgttgga agatcagata
    15061 aaagtaaaag tgccgataga agaaatgtat tatctgctac gtctagtcca taatcagtta
    15121 gataaaaaag aatacactgt gccatgaata cacaaaaatg atacactttt atagtgtatc
    15181 gtttttgtgt attggttaag aatcctttaa attcaattag tgtatcgtaa aaaagttggc
    15241 acgctttttg cttatagtag tgtgtgctag gaggtggttg ttcacacaga aatgataggg
    15301 gtttctgtga agtagtgagg aagaaggagt tttgcagatg gatattcgat tggcacgtat
    15361 agatgatcgt ttaatccacg ggcaagtcgc aacagtttgg tcgaagcaaa caggtgttga
    15421 acgtatcatt gtgatcagcg atgtagtcgc tcaagataaa ttgagaaagt ttctactaaa
    15481 agaagctgca ccgccgggga tcacagcaaa tgtaataacc gtgcagaaaa tgattcaaat
    15541 ctatcatagt ggattattat cacgagcaaa agtgatgctt ttattcacca atccccaaga
    15601 tgttgaagcg gttgtccgct gcggtgtaca gttgaagtcg ttgaatatcg gcggtatgag
    15661 ttttactgaa ggcaaatcaa tgattaccaa ttttgtttca gtaaatcaac aagatattga
    15721 ttcctttcgt tacttgaaca gtcaagaagt ggagttagag attcgaaaag tacctgctga
    15781 caggaaaatc cttttgatgg aactactagc gaaaatcaaa aaggtgtagc aagaatggtt
    15841 gttggaaaag gtcaaataat tctgttgtaa tggttagcaa ttattaatat ttttgaattt
    15901 gttacaacaa aagtaaatag gaggtataaa tatggtaggg attatcctag caagtcatgg
    15961 acaatttgct gaaggaatct tacaatccgg ttcaatgatc tttggcgaac aagaaaacgt
    16021 caaagccgtg attttgaaac caagtgaagg tccagatgac ttgagagcca aattggaaga
    16081 agcggttgct tcttttgata atcaagacga agtgttattc ttagttgatt tatggggcgg
    16141 aacaccgttc aatcaatcaa acacattatt cgaagagcac aaagataaat gggcaatcgt
    16201 cagcggtttg aatttaccaa tgttgattga agcctacgca tcacgctttt caatggaatc
    16261 tgctcatgaa attgcagcac acatcattga aacagcaaaa gacggtgtaa aagtaaaacc
    16321 ggaagaatta gaaccggcag aagcaccgaa agcagcagtt gaagacgcac aacctaaagg
    16381 agcacttcca gaaggaactg tggtcggaga cggaaaaatc aaatatgtat tagcacgtgt
    16441 tgactcacgt ctgttacatg gacaagttgc gacagcttgg acaaaagcag tgcaaccaaa
    16501 ccgtattatc gttgtttcag atgcagtatc aaaggatgat ctgcgtaaga gactaatcga
    16561 acaagctgct cctccaggtg taaaagcaaa tgttattcct attagcaaaa tgatcgaagt
    16621 agcaaaagac cctcgttttg gaaataccaa agcattgctt ttatttgaaa atcctgaaga
    16681 cgtattgaca gctgttgaag gtggcgtaga catcaaagaa ttaaatgttg ggtcaatggc
    16741 gcattcggtc ggtaaagtcg ttgtaagtaa agtattgtct atgggacaag aagacgttga
    16801 agcgtttgag aagcttgaac aaaaaggtgt gaaattcgat gtacgtaaag tacctaatga
    16861 ttcacgcgat aacatggatg atattttgaa aaaagcaaaa gctgaattag caaaagcgta
    16921 ataaataata ttaaaattta taggaggctt atcatgtcta ttatttcaat aattttagtc
    16981 gtattagttg cctttctagc tggtatggaa ggtattcttg atgaatttca atttcatcaa
    17041 ccactagtgg catgtacatt gatcggttta gttaccggta acctcgaagc tggtatcgta
    17101 cttggcggta cgcttcagat gatcgcttta ggttgggcaa atatcggtgc cgctgtagca
    17161 ccagatgctg cattagcatc agtagcatca gcaattattt tagttttagg tggacaaggt
    17221 gttaaaggtg ttccttctgc gatcgcgatt gccgttcctt tggctgttgc agggttgttc
    17281 ttaacaatga tcgtacgtac aatcgctgtt ccaatcgttc atttgatgga tgcggctgct
    17341 gaagaaggaa acatccgtaa ggttgaaatg tggcacatca ttgctgtatg tttacaaggg
    17401 gttcgtatcg cggtcccagc agcagcgttg ctatttatcc cagcacaaac tgtgcaatca
    17461 ttccttgaat caatgccagc atggttaaca gatggtatgg ctatcggtgg gggaatggtc
    17521 gtagctgtag gttacgcttt ggtaatcaac atgatggcta caaaagaagt atggccattc
    17581 ttcgttattg gtttcgttgt agctgcaatt tctcaattaa cattgattgc tctaggtgct
    17641 ttaggtgttg cattagctct tatttacttg aacctttcta aaatgggtgg ttcatcaaat
    17701 aatggaggcg gaagcaactc tggtgaccct ttaggcgaca ttttgaatga ctattaatct
    17761 tgaaggagga aaataaaatg gcagaagaaa aaatcaaatt atcaaaaaaa gatcgtttag
    17821 ctgttgcgtg gcgctctacc ttcattcaag gttcatggaa ctacgaacgt atgcaaaacg
    17881 gtggctgggc attttctatg attccagcaa tcaaaaaatt atacaaaaca aaagaagaac
    17941 gttctgcagc attaaaacgt cacttggaat tctttaatac acacccatac attgcttctc
    18001 caattttagg ggttacattg gctctggaag aagaacgtgc gaatggcgcg cctgttgatg
    18061 atgtagcgat tcaaggggtt aaagtaggta tgatgggtcc tttagctggt gttggtgacc
    18121 cagtgttctg gtttacagta cgtccaatgc taggtgcact aggtgcttca ttagctatgg
    18181 gaggaaatat ccttggacca atcatcttct tcttaggttg gaacattatt cgttgggcat
    18241 tcatgtggta tacacaagaa tttggctaca aagctggttc taaaattact gatgaccttt
    18301 ctggcggatt gttacaagac gtaacaaaag gtgcttcaat tttagggatg ttcgtattag
    18361 ctgcccttgt acaaagatgg gtatcaatca aattcttacc agttgtatca actgtaaaat
    18421 tagataaagg tgcttacatc gaatgggata aattgccttc aggtggtgaa ggaatcaaga
    18481 gtgcatttga acaagttaat aatggcttag ccctttctcc tgaaaaggta acaacattac
    18541 aagacaactt agaccaattg attcctggtt tagctgcatt aggattaaca ttgttatgta
    18601 tgtggttgct taagaaaaag gtttctccaa tcgttatcat ccttggttta ttcgtagtcg
    18661 gtgttgttgg acacgtcatc ggacttttat aatccataaa aatatagctc gggcgttgtc
    18721 ccgagctttt tttatatagt atactgttca taaacaaata tctggaggga gaaaatggtt
    18781 caatcaatca atacaaaagt agacttggta attgatgcta cttcgtttac gggtctgaca
    18841 gattacgggc aaatcatgat aggtgataat ggttttgagt tttaccatgc gcgagataga
    18901 cgcaaatata tccaaattcc atgggaagaa gtggattatg tactagcatc agtcatgcta
    18961 aagggaaaat ggattcctcg atatgccatt caaacgaaaa aaaatggtac atacacattt
    19021 tcttccaaag aacctaaaaa agtgcttcgt gcgatcagag aatatgtaga tgaagataga
    19081 atggtacaat cattgagttt ctttgatgta gtgaaacgtg gtattcgtag tatgtttaaa
    19141 cgcaagtaaa aaataaagaa aacttctaag aggaggtttt ctttattttt ttactttgca
    19201 gaagaaaata tgaaatagca aaaccaatta tttgtcccct ttaaatccca tatgctaaaa
    19261 ttgattagca aagaaggagg ttatattatg agttggttat ggacattgat tgttggagcc
    19321 gtgatcggag caattgcagg tgcgatcacc agtaaaggaa attcgatggg ctgtgtaacg
    19381 aatatcattg ctggactagt cggttcaagt attggacaag cgattttcgg cgaatgggga
    19441 ccaaaagctg caggtatggc acttattcca tctattttag gcgcagtgat tttggtagca
    19501 gttgtctcat ttttctttgg aaaacgatca taagttttta caaaatagta aactaaaagg
    19561 agttgcagcg agactgtgac tcctttttgg ctttatttat ttaaaataaa aagaagtttt
    19621 ccggaaattt ttatttaact gtcgtgtacg aagaaaagtt tgctataatg gaaaaaagcg
    19681 tgcaaagaaa gagggagttt cattatgatg ctacgtgtag aaaaattgcg caaaaaaatg
    19741 caagaagaaa atttggattc atttttggtt accagtccgt acaatcttcg ttacttaacg
    19801 aactttacag gtacgacagg attagctgtt attactttgg aaaaagcatt ttttattact
    19861 gattttcgtt ataccgagca ggcggcagcg caggctcaag ggtttgaaat tattaaaaat
    19921 gtcggaccta tttttgatga ggtagcaaac ttagtacgca aagaagaact gcactcatta
    19981 gcttttgagg aaacaacagt ctcattttta gaatattcgg ttttagaaga aatcatcgat
    20041 gcggaattag tacctgtttc acagatgatc gaagaactca gagaagtgaa agatgaagaa
    20101 gaaatcgcta ttatcgaaaa agcatgtcat attgcggata tggcatatga tcacatcctg
    20161 aaaatgatcc aaccgggcat gactgagatt gaagtcgcaa atcaactaga cttttatatg
    20221 cgttctcttg gcgcttcagg tgtttctttt gatacgatcg ttgctagcgg attgcgttcg
    20281 gctatgccgc atggtgtagc aagcaaaaaa atcatcgaac aaggtgactt gatcacattg
    20341 gactttggtt gctattacga agggtacgtt tccgatatga cacgtacatt cgctattggt
    20401 gatcctgggg aaaaattaaa agaaatttat caaatagtct tagatgctca attagcagtt
    20461 attgatgctg caaaaccagg aatgactggt gtccaattag atgcagttgc tagagattat
    20521 atttcaaaac atggttacgg ggaagcattt ggacattcta caggacatgg gatcggttta
    20581 gagatccacg aaggaccaaa cgtttcttct cgtgcagaaa agcaatttgt accaggaaat
    20641 gtgatcactg atgaaccagg gatctactta ccaggaatcg gtggagtacg tattgaggat
    20701 gacttgttga tcacagaaga tggaaatcgt gtgttgactc gttcaccaaa agaattgatc
    20761 atcttatagt ttcatcaata gaagaagtaa gcgtaaaaat cagtgttcac agactttgtt
    20821 atagaaaatc actcgattta atgatacact atagaagaac ttttaagctg taagagataa
    20881 taaagttggg tgttttctcc aattcatagc agtttgcagc tgatcggcgg cagttttctt
    20941 accacgtgat cactatgccg catcatagag aattcggatg tatctcgtgc ttaggcaaag
    21001 ggaggaataa ataaatggca gatgaaaaaa atttgatttt aaacgcaaac caagatttag
    21061 gagagatcgt gatcgctcct gaagtcatcg aagtaattat cgggattgca gcttctaaag
    21121 tagaaggcgt atacggaatg cgcggaactt ttgccagcaa tgtcactgaa ttattaggac
    21181 gtgcagctca cggaaaaggt gtttacttag caaacgagga agatggcttg aaagtagatc
    21241 tttactgtta tctagaatat ggtgtttcag ttcctaaagt agcaatggaa atgcaggaac
    21301 gggtcagcca gcaagtcctt ttcatgacgg atatcgattt atctgaagtc aatatccatg
    21361 tagtagctgt tgttcctgaa aaattagaac aaccaacaat cgaagaactt tttgaagacg
    21421 aagaggaaga gaatgaataa agaattatca agacacgaga ttcgggaaat ggcattgcag
    21481 gctttatttc cattagactt caatgcagac ctaacaaaag aagatgcgat tttcaacgca
    21541 atcgaattag accaccgcga catgatcaac gaagatgaat cagagtttgt tcctgtttat
    21601 cttgatacgc tagttggcgg tgtctgtgca aaaaaagatg aattggataa agtgattgaa
    21661 aaacatttaa aaaacaattg gcacatcaat cggatctcaa aaatggattt ggtgatcttg
    21721 cgtatcgcga tttttgaaat gacttatgtg acagatgtac cagcggctgt tgctttaaat
    21781 gaagcaatcg agttggcaaa aacatttagt gatgaccgtt cacgtaaatt cgtcaacggt
    21841 gttttatcca atgtattgaa agaattagaa gctggggcgt aagtcctagc ttttttctat
    21901 gaatcatcat aaacctgtta taaatcggga aaattctatg gtagaatgaa aacacaaaaa
    21961 atatctggga gtggttagat ggcagaattg attaacggaa aagaattagc tgaaaaaatg
    22021 caggcagaga ttgctgaaaa aatccaaaaa ttgaaagata atggtatcca tcccggatta
    22081 gtcgttttat tggtagggga aaatccagcc agccaaatct atgtaagaaa taaagaacgt
    22141 gtagcaaaag aaatcggaat ccattcattg gtagagcgtt atcctgagac gatttctgaa
    22201 gacgaactac ttgcagaaat tgaaaaatac aatcaagatc ctagttttca tggtatattg
    22261 gtccaattac cattgccaaa gcatatagat gaagaaaaga tcctgttagc tatccgccct
    22321 gaaaaagacg tagacggttt tcatccgatg aatttaggca gactccttgc gggtaaaccg
    22381 gacaagattc cttgtactcc atacggcatc atgaaaatgt ttgaagctta ttctatcgat
    22441 ccggcgggta aacgagcagt agttatcgga cgaagcaata tcgtcggtaa accgatggct
    22501 cagctactct tgatggcaga tgcaactgtt actgttgctc attctaaaac cgaaaatctg
    22561 gctgaactgg cacaagaagc agatatttta gtcgtagcaa taggacgtgg acattttgtc
    22621 acaaaagaat tcgtaaaacc aggtgctgta gtgatagatg tagggatgaa tcgtgatgct
    22681 aacggtaagc tgatcggtga tgtgaaattt gatgaagtgg agcctattgc aggctggatc
    22741 actcctgtgc caaagggtgt agggccaatg actatcacga tgctgatgta tcaaaccgta
    22801 aaatctgccg aagcttttgg agcaggtgaa tagatggcac aagaatattt aactgtcact
    22861 acattaacga agtatttgaa aagaaaattt gatgccgatc cttatttaga acgcgtatat
    22921 ttaacaggcg aaatatcgaa tttccgttta cgtgccaatg cccatcaata tttcagctta
    22981 aaagatgacc atgcaaaaat atctgctatt atgttcaaag gagcattcca aaaattacga
    23041 ttccagccaa aagaaggaat gaaagtcctt gtcgttgggc gtatttcatt atatgaagcg
    23101 ggaggttctt atcaaatcta tgtagagcac atggagcctg atggaatagg tgctttatat
    23161 caagaattgg aagaacgaaa agaaaagctg atgaaggaag gcctttttca agggcctaaa
    23221 aaaatgctgc cacgtttccc aaagcgaatc gcggtcctta ccagtccaag cggagcggtt
    23281 atccgcgata tcatcacgac ggtgaaaaga agatatccaa ttgtccaatt ggttttgttc
    23341 ccaacagttg tacaaggaaa ccaagcagcg gacaatgttg tcaggaatat ccagagagta
    23401 gaagagatgg gcaatttcga tacaatgatc atcggtcgtg ggggtggttc gattgaggat
    23461 ttatggccgt ttaatgaaga gcgggttgct cgggcgattt atcaagctaa tacgccggtg
    23521 atttcatctg taggacatga aacagatacc acgatcgctg atttagtagc agatgttcgg
    23581 gctgccactc caacagcagc tgcagaatta gctgttcctg ttttgtcaga agagttgatg
    23641 cgcattcaag aacggcaagc tagattgcag caggcgtttg tacgccagat ccagcgcaaa
    23701 caagaacggt tcgagcgtgc tcgacgttcc tatgtatttc ggcagcctga aagattgtat
    23761 gaaggtcaga ccatcaaatt agaccagttg aaacagcgat tatatcaagc tacacagcaa
    23821 atttatcatg cgaaagaaaa acaaacgatc gcattaagcc accaattgga acaagtggct
    23881 ccgctttatc gagttaaagc agctcgccaa gaaactgctt atctagaaaa acgcttagaa
    23941 gagaaaatcc aacaatatat gaaacaacag cagcagcgtt ttgctcaagc tgttcaatct
    24001 cttgatttgt tgagtccatt gaaaatcatg ggaagaggct acagctatac gacgttaaat
    24061 gatcaagtag taaaaagtgt caccgagatt catcaagggg atttgctgac gatccattat
    24121 caagatggaa tcgttaaagg acaagtacaa gagatagaca aaggagaata aaaatatgga
    24181 aaatccaact tttgaagagt cactgcaaga attagaaaaa attgtcatgc agttagaaca
    24241 aggagacgtt ccattagaat cagctctaga ttcgtttaaa cgaggaatgg aattaagcaa
    24301 acattgccaa gatacattag ctaaagcaga agagaccttg acgaaaatga tgacagaatc
    24361 aaatgaagaa gttgattttg acggaaacga ggaaagctga gatgagatta actgactttt
    24421 cagctattca tctacctgct gtagaaaaag aaatcttgtc ttttttagat gagcatacaa
    24481 cggatagagg actctatgat gcgatgagtt attcagtgaa agcaggcggg aaaagaatac
    24541 gtcccttatt acttttaacg gccgttgctt cctttgatga gcctattgat gttcccgtct
    24601 atcaagtagc agctgcttta gaaatggtgc atacgtattc cttgatccac gatgatttgc
    24661 ctgcgatgga caatgatgat cttcgtagag gaaaacctac caatcataaa gtattcggcg
    24721 aagcattagc tatattggcc ggagatggtt tgcttacagg agcattccag ctaatcagta
    24781 tggcacactt aggtaattct cctaagcttc tgctgcttca gcaattagct gtttgtgcgg
    24841 gatcacaagg tatggttgct gggcaagctg cagatatcga gggcgaatcg aaaaagcttt
    24901 ctttagaaga attagcattt attcatgaaa gaaagacagg gcatttgatt cgttatgcct
    24961 tgctggctgg tggcatatta gcgaagcaac ctgaagaaat cttattgcta cttcaacgat
    25021 tagccgaaca tttaggttta gcttttcaga taagagatga tctactcgat gtgattggta
    25081 caacgaaaac attagggaaa acagctggta aagatgaacg tatggaaaaa aatacttacc
    25141 cgcgtttatt aggcctagaa aagacacgtg aggcgttgga gattgagctg ttatccgcca
    25201 acaagatcat agacaaatta gaagaggacg ttttttcttt tgatggcggg ctgatgcgcc
    25261 agatgatcaa gcaatttgat gtggagaaat gagggaaaga atggaaaaag aacgcgtaga
    25321 tgtattagcg gtcaaacaag gattatttga aacaagagaa caagccaaac gttcagtgat
    25381 ggctggtttg atttacaacg aaaaaaacga gcggtttgat aaaccgggag aaaaaattcc
    25441 agtgagtagt gaattaagag tcaaaggtaa aaaacttcct tatgtttctc gagggggatt
    25501 aaaattagaa aaagcactca aagcatttga tctatctgtt gaaggaaaaa cattgttaga
    25561 tattggtgca tccactggcg gatttactga tgcagctttg caaaatggcg caaaaatgag
    25621 ttatgcttta gacgttggct acaatcagct agcttggaaa ataagacaag atcctagagt
    25681 cgtcgtaatg gaaagagtga attttcgtta tgcaaaaccc gaagacttta ccttaggaat
    25741 tccagaagtt gctgttatcg atgtttcttt tatttcacta aaactgatgc tgcctccatt
    25801 acatgctatt ttaaaagaag agggagaagt gattgcttta atcaaacccc aattcgaagc
    25861 aggcagagaa gctgtaggga aaaacggaat tgttcgtgac cctcagaccc atcaaaaagt
    25921 tttagaagac attttgtcat ttgcggcaca aggtggatat gatgtgctgg aattaagtta
    25981 ttctccgatc acggggggag aaggaaatat cgagttcttg gctcacttga ggaaagtacc
    26041 cgaaagcgga acaatcaatt cagctattaa tatggcagaa gtggtttcaa atgcacacga
    26101 acagtttgac cataaataaa ttgtaactgt gaaaatcaag tgagaagtat gcggattctt
    26161 ctttattata ccaaggaaat aagctatcat tgattaagaa gaatatgcta aggagcgaag
    26221 acgtaatgag aaaaaaagat cggcatcgtt tgattactcg attgttgagt gagcaagata
    26281 tccgaaagca ggaagaattt gttgaaatcc tgaaaagcaa aggaatatct gttactcaag
    26341 ctacgatttc cagagatata aaggaactga aactgatcaa ggtaccggct tcagatggcg
    26401 gctaccgtta tagcatacca gctgaaacaa acgaagatgt aaatactaaa ttggaaaaac
    26461 tattaaaaga cgctttcgtg gcagttgacc aaatggaaaa atttgttatt ttgaaaacat
    26521 tgccgggaaa tgcttcggca gcagcaaatt tgattgataa acgttacaag aaagaattat
    26581 tttcgattat caatgatgac gataatgttt tgatgattac acgaactgaa gaccaggcag
    26641 ttttattgaa gaaagacttc ctgcattacc tttaagttga ggagcgaatg tgatgctaca
    26701 agaaatcagt attactaact ttgcgattat tccggaattg cggctttctt ttcacgaagg
    26761 aatgaccgcg ctgaccggag agactggtgc gggtaaatcc attattatcg atgctttagg
    26821 tctgttagca ggaggaagag gttcaagcga ttatatacgt caaggagcag aaaaatgtgt
    26881 gcttgaaggg ttgtttgaac taccaaaaca agaaggattc tctgaactga tggttgaatt
    26941 agggatcgaa acagatgagg acaatctgat tgttcgacgg gatatgtcct taactggaaa
    27001 aaatgtttgt cgagtgaatg gtcatattat cacattagct aatctgagaa gaatcggcag
    27061 ttatttagta gatatccaag gacaaaacga acatcaggaa ctgctccagc ctgaatcaca
    27121 tcttgcttta ttggatcgtt ttggagatgt agctttccaa cagaagaaaa agagttatca
    27181 gcaagaatat atctcttatc gtgagctgga aaaaagggta cgaaaagtgc agcaaaatga
    27241 aaaatcctat gttcagagaa tagatatgct ccatttccag caagaagaaa tagctgctgc
    27301 tgatttgcaa gtcggagaag aagagaaatt aaaagaagaa agagaaaaac ttagcaatta
    27361 tcaaaaaatt gctgatggat tagctgcagg ttacggagca ctgacgaata atgaacaaaa
    27421 cagtgtagat ggtgttggct tggccgtttc agaactgcaa gggatcgccc atctagatgt
    27481 ggagtatgaa gcaatttatg aaaatatcca aagtgcttac tatttgctgc aagatgcaat
    27541 cggcgatatg agtcgtcaga ttgatttatt ggagttagat gagagtcgtt tagaagaagt
    27601 tacgcaacgt ttagaattaa tccgacagtt gaaacgtaaa tacggcgaat ccattgagtc
    27661 aatattggct tactatgacg aaatcaccga agagctagct tcttctgatt tttcagaagg
    27721 tcaattggac aaaatgaaag aggaactaga gcaaaaagag cttctacttc agcaacaagc
    27781 cgcagatctt catgaagcgc gtaaaaaaat cgcaaaagaa ttggaaaaat ccattctgca
    27841 tgaattgaaa agtctgtata tggaaaacac tgagtttgag gtacgtttct taaaagaaga
    27901 aaatagacaa ttgaatcatg atggatttga ccaaatcgaa ttttacatta cgaccaatcc
    27961 aggagagcca ctgaagcctt tggtgaaagt cgcttctggt ggcgaactgt ctcggatgtt
    28021 gcttgctttg aaaactattt tttcttctga gcaaggtgtg acaagtatta tttttgatga
    28081 agtggataca ggtgtgagcg gaagagttgc acaggccatc gcagataaaa tttctcaaat
    28141 atcgaaatac tctcaagttt tgtgcatcac tcatctgcct caagttgctg cagtcgccga
    28201 ctatcaatat tatattgtga aagcagtgat tgatggacgt acgcaaactt ctgtttcaga
    28261 gttgaaaaca aaagaaagag aagaagaaat tgcacgtatg ctggctggaa gtgagattac
    28321 aaaactcaca ctcgaacatg caaaagaact attgaaactc gcaaaaagga cctgagccac
    28381 ctcgcagtgg ttcaggtcct tttcagcagc tgatagctat attttatttt tacatgacac
    28441 tcattacagc agtcaataca acagatccaa tcgttaaaat cagcattgtc caaatgacaa
    28501 attttgttac tttagcaaaa gtacttgttt gttttttttc acgcattgtt ttcaacctcg
    28561 tcattaatta gttgattcaa gtgtacaatc ttttttccat ttccacaaga gggctttttt
    28621 ataaaaactt cccttttttt aaataatagg tagttacgat acaaataaat aatgttaata
    28681 gaactagcca tataaaggca tcatcccttt cagcaatagg taacttaatg ttcattccat
    28741 agatactgcc gacgattgtc ggtatagtta aaataatcgt taaagaagtt aaaattttca
    28801 taatattatt cagattattc gatacgattg ctgaaaaagt ctgattcatt tgttctacta
    28861 gttttaattg aatgcgagaa gtagtcattg cctgctctgt ttccaccaat acatcacgta
    28921 aatgattttt caaagcatga ttttcttgga aaagtttatt ttgcgctagt tctttcaatg
    28981 catctaaatt agcagaagtc gcttctctga aataaacgag gcttttttgg atatccatga
    29041 tttgataaag ttgtttgttt tcagtggata cttgaagttt ggcttccaaa gtatcgcatt
    29101 gagattgaag cgtagctaaa taactgttgt aagagagtgt gacctgccaa atgaattgaa
    29161 agaagatatt gagattaggc gaaatatcac tttttggaaa ggtctggcta aagactttct
    29221 ttaaaaacag tggttcatga ttcgtaattg tgataagctt ctttttgggc gtcaaaataa
    29281 tagagagcgg gtatgtatcc acttgaaaat aaccgctagg acttgtttta gcaaaaggat
    29341 attgtaacaa taataacgct ggctttgtca actttgattg attgaattct tctgtacggg
    29401 aattttctgc atcgtccaat atggaagtga tgtaatctga cggaatttca aattgatgtg
    29461 tcagctgatg aatctcttct tgattaggac gttctacaca ataccagatc gtttggtcag
    29521 tttcctttcc ggtattacgc agtttctcat tatctaatgt aaaatatttg atcatgattt
    29581 tcctcctgtt gcctgtttct tttattataa cggataggga attggaatac atggaatgtg
    29641 tttgtaagat atagaatata actgcataaa tttaaataaa atgaaacagt tgtgtaaagt
    29701 atatacagta tacttgaagc aacgataaaa aaggagttta cgttcatggg cacattcaca
    29761 ccacttatct tgttttggta tatttacccg gtaatcgttt tgtttggttg ccaatttctt
    29821 gtaaaattat tttcgttaaa tcgtcgtttt aaaataaaag cccctgattt ggcgattcct
    29881 tttttgtggg gcggtatgca tgcattgtct agaaatacag ggacaatttc tatcttaccg
    29941 tttttcttta tttctgtttt gctaatggga atttgtattg ctgttttcca agcatattat
    30001 tatgaagaaa ttatttatcc gcgttatttc aagatgactt ggcgtttggt ttttctgttg
    30061 acaatcgtct tgtacgctgt cttgatcatt gtgaacatac tttcttacct atgaaaaaaa
    30121 tagaaaagct gtaaaaagtc ttttaaaagg ctttttgcag cttgattttt aaaaaaatgt
    30181 ttatgaataa aaaaagttgt tcccggattt cccagaaaag gtggtagaaa gtgggggatt
    30241 gtggtagact aattttagtt agtggaggaa tggggggctt tcaatgttga tgggtgaatt
    30301 ccaacataat attgatgcaa aaggacgttt aatcgtgccc tcaaaacttc gtgaagaact
    30361 tggcgaaaag tttgttctga cacgcggatt ggatggctgt cttttcggct atccgatgag
    30421 cgaatgggaa aatctggaag ctaaactgaa cgaaatgccg ttggctaaaa aagacgcacg
    30481 tacttttgtg cgattcttct attcggctgc cactgaatgc gaacttgata aacaaggacg
    30541 aatcaatata cccagcacgt taagaaatta tgcagctttg acaaaaggtt gcgtagtcat
    30601 cggtgtatct aaccgaattg aaatttggga tgagacacga tggcaagatt tttctgcagc
    30661 agcagaagag aactttgacg aaattgctga aagtatgatt gatttcggac tataagaata
    30721 ggaagaggct gactaatgac tgaaacattt caacactata cagtcatgtt gaaagaaacg
    30781 gtagatggat tgaacatcaa accggatggc gtttatgtag actgtacact cggcggtgcc
    30841 ggacatagtg aatatttatt aagccaacta agttctaaag ggcatctgta tgcgtttgac
    30901 caagatcaaa aagcaatcga tcacgccaag ctgcgtttag cttcttatgt cgaacaaggt
    30961 atggtcacat ttatcaaagc gaacttcaga gaattagaag aacgcttgag tgagcatgta
    31021 gataaggtag acggcatttt gtatgatcta ggggtttcct ctcctcagct agatgaagca
    31081 gaacgtggat ttagttatca tcaagatgca ccgttggata tgcgcatgga tcagtcagct
    31141 cctttatccg cttatcacgt aattaatgaa tattcctacc atgaattagt aaagattttc
    31201 ttccgctatg gcgaggagaa attttccaaa caaattgctc gtgaaatcga acgagtacga
    31261 aaaacaaaac caatccaaac aactggtgag cttgtcgaaa tcattaaaca agtcatccca
    31321 gctcccgcaa gaagaaaagg cgggcatcct gcaaaaagaa tttttcaagc tgtgcggatt
    31381 gcagtaaatg acgaactcgg tgccgaagag gcctctttag aacaggcaat ccggttattg
    31441 aaagtcaatg gaagaatcag cgtaatcaca ttccattcat tggaagacag aattgtcaaa
    31501 agtatgttta aagaatatag tacggtacaa gatctaccac cgggacttcc tgtcatacct
    31561 gacgaattcc aaccagaact gaaagtcatt actcgtaaac caatccttcc ttcagaggaa
    31621 gagctaacag aaaacaatcg atcacgcagt gcaaagttac gtattgcaga aaaaataaga
    31681 atgaaagagg agtagaatga tggcagaatt aaagaaaatg caagattacg aatatgattt
    31741 acctgaattg gtggatcagc cggaacaaca aatgcctgat tcttcttcga atcaagaaat
    31801 cattcttcca caatcaccgg caaaacgatt aaagcagatt tctgctttgg aaaaaattgc
    31861 tgtggcaagc gtggcgattg ctattgttgc tttatgtgtt ttaacagtta tgctgcgcac
    31921 aaatatcagc ggagctgaaa aagaaatcac gtcaatacaa acagaaatca atgataaaaa
    31981 gcaagaaaaa acgagtttag aacaagaaaa aaacgaactt tctcgtactg aacggattaa
    32041 acaaatcgca gaagaaaaag gattgtctat taatgacgac aatttaagga aagtgaagta
    32101 aatgagtctt aaaaataaaa ttcggcggtt tatggaaaag aaaaatctaa atccgatgaa
    32161 caatcgcaaa aaagtaggta tcattttatt tgctacgagt attggattgt tctttttatt
    32221 tgccgtccgt ttctcgtata tcgttattgg aggtcatgtt gctggtacct cattagctga
    32281 aaaaacaaaa cagttgtacc aaggaagtga agtagtaaaa gcaaaaagag gaacgattta
    32341 tgatcgtaac ggtgtggcgc tcgcagaaga tgcctcctct tattcaatta aagcgatact
    32401 ctcaaaaaca tatacctctg gggataaaaa actgtatgtt gaagaaaaga attttgataa
    32461 aatagcagaa atcctgcata agaatctttc tatagacaaa aaagatgcac tgaatatttt
    32521 agaggacggc gcaaaaaaag agctgtatca agttgaattt ggtagctatg ggaaaaatat
    32581 ctcccaagaa accaagcaaa acatcgaagc ggacatgaaa aaagaaggtg tcgctggtct
    32641 ttattttgtc gaccatcagg ctcggatgta tccaaatggc gtcttttctt ctcattttat
    32701 tggatacgca gttccagaca aagatgagaa tggcttagtt ggtaaactag gtttggagtc
    32761 tgcttacaat gatatcttga gcggtaaaga tggtaagatt atttatcaaa aagataattt
    32821 tcaaaatcct ctgccaggta cagtggcaga agaagaaaaa gctgtcgacg gacaagatat
    32881 ctacacgact ttagacagca gactacaaag ttatttagaa acactgatgg atcaagtaaa
    32941 tgaagaatat cagccagaag aattgacagc agtcctgatg aaagcaaaaa ccggagaaat
    33001 cttggcaatg ggacaacgac ctactttcaa tcctgaaaca atggaaggat tgactggaaa
    33061 agatgctatc tggcgtaatt tcttggtaca agatagttat gaaccaggat caaccatgaa
    33121 agttttcact actgcagctg ctatcgaaga aggagaattc aacgagaatg aaaccttcca
    33181 atctggaaaa atccaagtag aagatgctac gatcaatgac catgactttg gtgaaaaagg
    33241 tgtgctgacc atgcgtcaag ctctttcatg gtcaagtaac gtcgggatgg tcatactgga
    33301 acagcgatta ggcggacgat ggtataacta tttacaaaaa ctgggatttg gacaaagtac
    33361 tcattctgga ttagatgatg aagtaaacgg tgctttacct acattaaata ttgtcgaccg
    33421 agcaatgagt gcttatggac aagcggtagg tgtgacgaat ttccaaatga tgaaagcttt
    33481 cacttctatt gcgaataacg gtacaatgat tcaacctcga tatatcagca aagtggtgga
    33541 tccccaaact ggagaagagc gaacaactca aacagaagtt ctagggcaac ctttttcaaa
    33601 agaaacaaca gaaaaagtcc gtgaatatat gagagatgtt gtcgaaagcg aaaactacgg
    33661 tagtgcttat ggtgtatata gtgtacctgg atacaatgta tcggcaaaaa ccggaacagc
    33721 ccaaatcgct tctgatacag ggggatatca aacaggagat acagcctacc cgtattcgat
    33781 tgtggagatg gttccttctg aagatccaga ctatgtcttg tatctaacaa tgaaacaccc
    33841 taagacatat gatcgaatgg cattggctaa aatcgcaaat ccattgatga aacgggcaat
    33901 ggattttaaa gaaactgaag aagattcaga tacagaaaca aaaacagaaa aagtatccgt
    33961 agccgattat cgtaatcttg aagcggacgt tgcagcagct gatgctcaaa aaagtggact
    34021 gcaacctgta gtgatcggta atggtaaaaa agtacaaaaa caatctactg ccaacggtga
    34081 tcagttgatc tctggagaaa aactgatcct atatactggt ggtgataagc tgatgcctga
    34141 cgtaacagga tggtcaaaag cagatatcat gaagctgggg aaaatcctcg gtattgaagt
    34201 tagttttgat ggagatggtt attgtgtcaa acaagaactt gctccatatg aaaaaatcac
    34261 tgaagacaaa cttagtttta ctttagaaga ataagaagag aaataggaga gaaaagagca
    34321 tggaatggac acaagcattg attcccattg tcagcagttg tgcaatgaca attgccgcaa
    34381 tgccgctatt tattggttat tttcaaatga aaaagcaagg tcaggcgatt cgtgaggagg
    34441 gaccaaagtg gcacaatagc aaagccggaa ctcctacaat gggcggctta gtgtttctga
    34501 tcggctctat tttgacagga atttgggttg gagcttggca aaaacagcta actcctactc
    34561 ttttcatctt gctttttgta ttagcgttat acggagtaat cggctttctt gatgatttta
    34621 taaaaatatt caagaaaaga aacatgggac tgaactcgaa gcagaaattg attggacaga
    34681 ttctaggggg aatcatcttt tatcttgttt accgttctga aggttatcca ggtgtattga
    34741 atttcttcgg actagatcta cctttaggaa tcatttatgg tattttcgct ctcttctggc
    34801 ttgttggatt ttcaaatgca gtaaatctga cagatggcat cgatggatta gttgctggct
    34861 taggaagtat ctcttttgca gcttacggtc taattgcttg gcatcagcat cagtatgatg
    34921 tattaatcat ttgcttaagt gttttaggag gtttactggg tttcttcgtc tacaacagaa
    34981 aacctgccaa gatttttatg ggagatgtcg gttcattagc gttgggcgga cttttagcgg
    35041 caatttctat aatgctaaac caagaatgga cattgctttt gattggtttg atctatgtta
    35101 tggaaacagc aagtgtgatg cttcaagtta cttcctttaa gcttacagga aaacgaattt
    35161 ttaaaatgtc tccaattcat catcactttg aaatgtgcgg atggtcggag tggaagattg
    35221 atacagtttt ttggttgatc agcattgtga cttcgttgat cacattatgg atcgtttggt
    35281 aggagatgac gggaaatgaa aaaaatatca acatatgaaa ataagaaagt gcttgtctta
    35341 ggactcgcaa aaagcggagt aagtgcggcc aaattattac atgaattggg cgcacttgtc
    35401 accgttaatg atggaaaacc ttttgacgaa aatccggaag cacaagagct gctttcttta
    35461 gggataaaag taataacagg tagtcatcca atcgaattgt tggatgaaga attttcattg
    35521 atggtcaaaa atccagggat tccctacagt catcctttag tagcaaaagc ccaagaaatg
    35581 ggtattccgg taattacgga agttgagcta gcttacgaag tagcggaatg cccaattatt
    35641 gggattacag gaacgaacgg aaaaacaacg acaacaacga tgacaggact actactgaat
    35701 gcaggagcag atcaaggaat tgcacgttta gcgggtaata ttgggtatcc agcaagtggt
    35761 gtagcgcaag aagcaaaatc agaagataaa attgtgatgg aattgtctag cttccaactc
    35821 atgggaatca cagatttccg ccctcatata gcggtgatta ccaatatcta tgaagcacat
    35881 attgattatc atggtacaag aaaagaatat gtcaaagcta aatggaacct tcaaaaaaac
    35941 atgacagaaa aagattatct gatccttaac tggaatcagt cagaactaca agaattagcg
    36001 caaagaacaa aagcacgcgt actgcctttc tcgacaaaag aagtgttgga agacggtgtg
    36061 tatgctgacg attactcaat atactataaa aaagaaaaaa taatggaaat atctgaactt
    36121 ggcgtaccag gtaagcataa tgtagaaaat gcactagctg ctatttctgt agcaaaattg
    36181 tatggtatat caaatgaagc tatacgagaa acgttgcatt ttttccatgg cgtaccacat
    36241 cgtacgcaat atgtaggaga aatccaaggc agaaaatttt ataatgattc aaaagcaacc
    36301 aatattttag cgactaaaat ggctctaagt ggatttgaaa cgtctaaggt cgtgttattg
    36361 gctggcggat tagacagagg aaatacattt gatgaattaa ttccttcatt aaaaggaatc
    36421 aaagcaatgg tcgtatttgg ccaaacaaaa gaaaagttga tggatgctgg aaaaaaagca
    36481 ggcatcgaga caatagtaac agccgattct gtagaacaag cagttccttt agcattagaa
    36541 aacagtacag atggtgatgt agttttatta tcaccagcga atgccagctg ggatcaatac
    36601 ccaaattttg aaactagagg aaacagattc atggaagctg tgaaccgatt aaaatagaaa
    36661 aagtagaagc gagcgataaa aaatgaaaat tttggttaca ggcggaggaa cagggggaca
    36721 tatttatcca gctcttgcct ttgtcaatta tgtaaagact caagaaccga atgctgaatt
    36781 catgtatgtg ggagcaaaac gaggtctaga aaacaaaatc gtccctgata caggaatgcc
    36841 tttccatacc ttggagattc aaggatttaa acgaaaaatt tccatgcata acatcaagac
    36901 ggtccgattg tttcttaaga gtatccgtca agcaaaaaaa atcctcagtg attttcaacc
    36961 agatatcgtt atcggtactg gaggatatgt ttccggagcg gtagtgtacg ctgcagccaa
    37021 aatgggtatt ccaacagtta tccatgaaca aaacagtgta ccaggtgtta ccaataaatt
    37081 tttaagccga tatgtggatc ggatcgctct ttcatttaaa gatgcggctt ccttttttcc
    37141 tgaaaacaaa gcagttcttg taggtaatcc tcgagcacaa gaagtagcag atactaaaaa
    37201 atcagatatt ttgaaaacat ttgatttgga tccagaaaag aaaacagtct tgatttttgg
    37261 cggtagtcaa ggagcactta aaatcaatca agcagtatca gtttttttaa gtgaatatat
    37321 tccttcttct taccaagttt tgtatgcttc aggagataga tattatgaag agatcctaaa
    37381 taaaataaat cctgaaatac aaaatattag tgtccagcct tatatcaaaa atatggcaga
    37441 ggtcatggct tccagtgatc tgctggtggg gcgtgctggt gcaacttcga tcgcagaatt
    37501 cacagctctg gggttaccgg cagtgttggt tcccagccca tatgttacga atgatcatca
    37561 gactaaaaat gcaatgagtc ttgttcacgc aggagcagca aaaatgattg cagataatga
    37621 actaacaggt gaatcactaa gtcagacagt caacgaaatc atgggtgatg aagaattgca
    37681 aaagcaaatg tgccgagctt ctaaagaaca aggaatccct gatgcttcaa aacgtcttta
    37741 tgacttagta aaacaaataa tcaaaaaata aaggcggtgg tcttagtgag taagaaaaaa
    37801 gaaccaaaaa aattgtctca tccgtcttct gatgaaaaca atttgactcc ttggcaaaag
    37861 gctaaccgaa aatatctggc agaacaagag atgaaaaacc aagagccaaa aaaagaagac
    37921 ataacattag atacacccct ttcagattct gaagaagaaa aaacggttca agaggaacag
    37981 caagaagaaa gccagtccga gaaaaaaatc gaattttttg aagagatcaa gcaaaagaaa
    38041 aacggtggac catataacgg ttcttttttg aaccggctgc ccaatttaaa aagtcaacgg
    38101 cacaaagtac tatttcgtcg gttagctttg atcatcactg tattgggtat accgctgatt
    38161 tttttgatct attatgtttc tccttatagc aaattacaag cagttgcagt atcaggcaat
    38221 aagacagtca acagccaaga aatcatctct gatacaaaat tgtcattagg agaaaatgtt
    38281 tggggacagt attttcatcg atctacttat atcgatcgcc ttaaaaaagc acagccaaga
    38341 atcgaaacag caaatataca tttcaaaggg atgaatgaat tcgatttgga tgtaacagaa
    38401 tataaagaga ttgctttgat cgcaaaaaac aatcaatact acccagtgat tgaaaatgga
    38461 acagtattag atgaaaaagt agcaaatcct acaaaaaatc tccctatctt ggaagagttc
    38521 aaagactctg caaaaatcaa agaattaaca aagcaataca atcagttatc atcagaatta
    38581 caaaaagcaa tttctgaaat caagtacacg ccaagagcaa gcaataaaaa tctgatccaa
    38641 ttgaatatga atgacggcaa tcaagttatt gtaaatatca ataatcttgc gaaccaaatg
    38701 aaatattatt cgcaagtagc caaagatatg gatgaaaaag gtgtgatcga catggaagtt
    38761 ggtatcttct cgcatcctta cggtagcact caagaaacgg aagaatcggg ccaatcggaa
    38821 gaaacaactg aaagtgaaga aaatgcggat caaacaaacg aatcaagtga agaggcagaa
    38881 acgcaaaaag cacaaacacc tggatcgtct agcacaaacg gatcatctga ttcaaatgat
    38941 tcaagtgaaa caagtcaaat agaagataat ccaagtacaa ctccgtcaag tttaaatgaa
    39001 aattgataaa acaactttaa aaagtaagaa tgtactttct aaaaaccagt aatttatggt
    39061 agaattggaa gcagtgaata tatctattaa taagtatatt ttaaaacgga cgctagttgt
    39121 tcgcttgtat atggaatagt aagtaggagg gaatcccatt catggcaaaa acaggaatgt
    39181 atgtaggcct tgatattgga acgacatctg ttaaagttgt cgtggctgaa tatatcgaca
    39241 gccaaatgaa tattattgga gtaggaaacg caaaatcaga agggattaac cgaggcatta
    39301 tcgttgatat cgataaaacg gtccaagcga tacaacgagc agtacgacaa gcagaagaaa
    39361 aagcaggtat ccagatcaaa ggcgtaagtg tcggtttgcc agcaaacatg cttgaagtag
    39421 aaaattgcca gggaatgatt gcagttaacg gagattcaaa ggaaatcacc gatgaagatg
    39481 tcagaaatgt ggcttcagct gcccttgttc gttcaatacc tcctgaacgt caaatcgtgt
    39541 caatattgcc acaagatttt actgtcgatg ggttcgaggg aatcaaagat cctcgtggaa
    39601 tgatcggcgt aagattagaa atgtacggtc tgttgtttac aggacctaaa acgatcatcc
    39661 ataacatacg taaatgcgtg gaaaatgccg gattaatcgt caacgaaatg gtgattactc
    39721 ctttagcgct gactgaatca attctttctg acggagaaaa agattttggt acgatcgtca
    39781 ttgatatggg tggcggccaa acaacaacgg ctgttatgca tgataagcaa ttgaagttta
    39841 ccaatcttga ccaagaaggt ggcgaatttg tcactaaaga tatctctatt gtattaaata
    39901 cgtcatttaa caatgcagaa gcgttgaaaa taaactacgg ggatgcatac ccagaacgga
    39961 catcaccaga tgaagaattt ccagtagatg tgattggtca gtcagaacca gtgaaagtcg
    40021 atgaacgcta cttgtcagaa attatttcag ctcgaatgga acagattttc aacaaagcaa
    40081 aagatgcatt ggaccaaatc gaagcattag agttgccagg tggcgtcgta ttgacaggcg
    40141 gagctgctag tcttcctggt gttgtggact tggcacaaga aatcttcggc gtaaatgtaa
    40201 aattgtatgt tccaaatcac atggggctga gaaacccggt atttacaaat gtaataagta
    40261 tcgtggatta ttcagcaaac ttgagtgaag tgtatcaatt ggcaaaaatt gctgttacgg
    40321 gtgaaacctc agcagctcgt caaatggtag ttgaacaaga aacaacaaac acatatgaga
    40381 gctatgaagc acctgaagaa acaatctacg atgagccgga accaaaagag tccggagaaa
    40441 atgtaaaaaa taaaatcaag ggtttcttta caaatatttt tgattaattc atcctgggag
    40501 gaataagata tggaattctc aattgataat aatatcaatg acggcgcagt aatcaaagtc
    40561 atcggagtcg gtggtggcgg tggaaatgct gtaaaccgaa tgattgaaga aaacgttaaa
    40621 ggtgttgaat tcattacagc caacacagac gtgcaagcat taaaaaattc aaaagctgaa
    40681 acagttatcc aattaggccc caaatatact cgtggtttag gtgctggttc tcagccagaa
    40741 gttggacaaa aagcggcaga agaaagtgaa caatccttac gcgaagcttt agacggtgca
    40801 gatatgattt tcatcactgc aggaatgggt ggaggaaccg gtacaggtgc tgcaccaatc
    40861 gttgcaggta tcgcgaaaga attgggcgca ttgactgttg gtgtcgtgac acgtccattc
    40921 acttttgaag gaccgaaacg tggccgtttt gctgcagaag gtatcgctcg tttgaaagaa
    40981 aatgtggata cattattaat tatctcaaat aaccgtttat tagaagttgt agataaaaag
    41041 acaccgatgc ttgaagcttt ccgtgaagca gataatgttt tacgacaagg tgtacaaggt
    41101 atctctgatt tgatcacagc gccaggttac gttaaccttg actttgctga cgtgaaaaca
    41161 gtgatggaaa accaaggtac agcattgatg gggatcggtg tggctagtgg agaagatcgc
    41221 gtagtagaag caacgaaaaa agcaatctcc tctccattgc ttgaaacatc tattgatgga
    41281 gctgaacaag tacttcttaa catcacaggc ggtttggata tgacattgtt cgaagcacaa
    41341 gatgcttcag atatcgtagc aaatgcagca actggcgatg taaatatcat tctaggtaca
    41401 tctatcaatg aagaaatggg cgatgaaatc cgtgtgacgg tcatcgcaac aggtatcgat
    41461 gaatcaaaaa aggaacgcaa gtctagccga ccagctagac aagcacaaat gcaatcacct
    41521 gctcaaaaga ctgtattaga tatggatcaa gctaaaccaa cttcttcaga agaagaaaac
    41581 agttttggcg attgggatat cagacgtgaa caaaatgttc gtcctagagt tgatgattcc
    41641 aattttgaca atatcgaaaa aaaagaattt gatacattca accgcgaaga aacaaaatct
    41701 catggcgatg atgaattaaa tacgccaccg tttttccgtc gtaaaaaata aaggagagga
    41761 agagcacatg attgctgaca acttgcagaa aatcaagcaa gaaattcagg attcgtgtgc
    41821 tcttgttcat cgtgatccga aagaagttac attagtagct gtaacaaaat ctgttgaatc
    41881 cgctcaagct gaagaattgg ttagagctgg cgttatgaat tgtgcagaaa accgagtaga
    41941 taaattatta gaaaagaaac aagatttatt tgattttcct gaaataaagt ggcatttaat
    42001 tggtaattta cagcgcagaa aggtaaaatt gattgtaaat gaaattgatt actttcatgc
    42061 gttggacagt ttaagattag cagaagaaat ccagaagcgg ttagagaaag aattatcctg
    42121 ttttattgaa gtaaatgttt ctggtgagga atcaaaacat ggaatttcac cagatgagct
    42181 tttaccgttt gtggaatctt tagctgcctg cacaaaaatc aaggttgttg ggttgatgac
    42241 tatggcgcct aaagaagctt ctgatgagca aatccataaa gtgtttggta cgctgaaaga
    42301 attgcgtgat cttgttcaag caaaaggtta tgcccatgca ccatgtagtg aattaagtat
    42361 ggggatgagt caagactttc aaattgcgat tgaagaaggt gcaacattta tccgtgtagg
    42421 aacagcgcta ttcaaagacg aataaaggag gaacaaaaat gccgctttta aacagaggag
    42481 caattgctaa ttttttcgga ttagctgatg aggaagagta cgaataccaa gattatcctg
    42541 aaacaacaaa aaaggctgta cagccaacta aaaaaaatac aacacagaat caatcaactc
    42601 attctgtatc tgctgcgaat actcatgaaa aacaaaaaac ggcgacttca cgtcctgtaa
    42661 cgcatgaaag tattcagccg gctgaaaaga aaaaagtaat tgaactacat caatcaccta
    42721 caaaaactgt agcgaaagaa agaacagctt caagaacctc tacacaacct aaagcaaatc
    42781 ttgctggaaa aatcactgta atggaaccaa gagcttattc tgaagctatg gcgattgcta
    42841 aacgaatcat tgctggagaa gcaaccttag tgaattttca tttagtagaa gaaaaacaag
    42901 cgagaagaat cgttgatttt ctaacaggaa cagtttatgc actagatgga gatatcaaac
    42961 gtgttggaga tgagattttt ctttgtacac cgactgggat ggagattgac agttcaaccg
    43021 ctcaatcttt tgcagatgcg aatttgtttg atctgtaaga ggaggaacta cgatttatac
    43081 tattgcacta ttacttcatt tacttcaacg ggcagcctat ttctatagca ttatcttgat
    43141 ggtatatgca ttgctctctt ggtttccagg cgggtatcaa tcagggctag gacgattatt
    43201 agctaagatc tgcgaaccgt atatcagttt gttcgatcgt ctgcctttga ggattggacc
    43261 cttggatttc acaattttag gggcaatcct gatcttgaac ttagccgtac aaggtttagc
    43321 acagatcgtt atatggataa ctgtccattt agtttattga ttgaagcaac aaaatcttta
    43381 ttctcagaat agataggagg ggaaaatttt ggatgcaaat gtataccagc actttcgggc
    43441 agatgaacgt acatttatcg attcggtagg cgattggatt gaacaggttc aaggacaata
    43501 tgcaccctat ctgaccgaat ttcttgaccc aagacaatcc tatattttag aaacactgat
    43561 tcgacaaagc agtgatttgc gtttcatgtt ttatggcgga tatgaacagg cagaaagaaa
    43621 aagatgtttg attttccccg attattacga acctgtagag gaagattttg aaacagcatt
    43681 gtatgaaatc cattatccta gcaaatttgc cagcctaagt catgggaaaa tccttggaac
    43741 tttagtggga acggggatca aacgctctta ctttggcgat atcatctcgg acggtgatcg
    43801 ctggcaagta tttattgcaa atgaggtgga tcattttgtt gctgcacaag taacaaagat
    43861 tggaaaggtt gctgtacgct tggaaccaat caaatacaca gaaatgatta tgccgaaaga
    43921 tagctggaca caagaaagag gaacagttag ttccatgcga ctagacagtg tgatctctga
    43981 gatttataat atttccagac agcggtccaa gcagctgatc gaagctggta aagtaaaagt
    44041 gaactggaca gaaaatacaa gacctgattt tgtacttgaa ctattagata ttatttcagt
    44101 cagaggttat ggcagagtcc agatccaaga acttgagggg aagacgaaaa aagaaaagta
    44161 cagggtactg ttcggtgtat tacgtaaata aaataaaaag gtggtttact catggcttta
    44221 actccattag atatccaaaa taagacattc ccgacaaaaa tgcggggata taatcaagac
    44281 gaagtagatg atttcttaga tttagttgtt cgtgactatg aggaattgac tcaaagaaat
    44341 cgagaattag aaaaagcagt caaacattct gaagaaaaac ttgagtactt caatgaatta
    44401 aaagatgctt tgaaccaatc aatcatcgta gctcaagata cggctgataa agtaaaaaca
    44461 agtgcaagca aggaatccga agtgatcgtt acttctgcac aaaacaaagc agatgagtta
    44521 gttgcgaacg ctgaaaaacg tgcacatcaa ttaacaactg atgcagaaga aaaagcaaga
    44581 aaaatcttga cagatgcaac agaaaaagca agacagcttg cgacagaaac tgaagatctt
    44641 aagaaaaaaa cacgagtatt ccatcaacgt atcagcttga tgcttgagtc tcaattagaa
    44701 caagtgaaaa gtccggaatg ggatgaaatt ttacaaccat tctccagcta cgtgactgac
    44761 agccatgaag tgatcaaaga agttttagca aaacagcttg acaatgaaaa tgatactgat
    44821 gtaaactcag acacaggagt catagaagca cctatgacgg catttgatac acaggctatt
    44881 gatatctcga tcgtgcctgg tgaaaatgaa aacgagtaga acagaaaagc actttgttat
    44941 tcttaaagcg agccgatgag agtggaagtt cggtaagaca ctgcatggtg aaagatcctc
    45001 tacttgagac ctgcttttcc gaaattagag taagaagcag cggctgacct cgttattggt
    45061 cacagagagt ttgcactaga tagatgcaac taaatttggg tggtaccacg gcatgttcgt
    45121 cccttgcggc tgaacatgct tttttgtgtc cggaaaacaa ataaaggagt gtatggttga
    45181 atgaaaatga aagaaacctt gcaattagga aaaacaaaat tccctatgcg tggtaatttg
    45241 ccaaaccgag aaggcgagtg gcaaaaagaa tgggaagaaa atcgaatcta ccaaaaaaga
    45301 caagagttga acgaaggaaa acctagcttt gttcttcatg atggacctcc atatgccaat
    45361 ggtaatatcc atttaggaca ttcattgaat aaaatcagta aagacattat cattcgttca
    45421 aaatcaatgt ctggctatcg tgcaccatat gttccaggat gggatacaca tggattgccg
    45481 attgaacaag tattggctaa taaaggcgtg aaacgtaaag aaatgtccat ggcagaatac
    45541 ttggaaaaat gtcgtgaata tgcactttcc caagtagata agcaacgagc agatttcaaa
    45601 cgcctaggag ttgcaggaga ttgggaacat ccttatgtta cattagatcc ttcttatgaa
    45661 gctgcacaag tacgcgtatt tggtaaaatg gctgaaaaag gctatattta caaaggattg
    45721 aaaccaattt actggtctcc gtcaagtgag tcttctttag cggaagctga aattgaatat
    45781 aaagatgtaa aatctccatc tatttttgta gctttcaatg tcaaagacgg caaagatatt
    45841 cttgatgaag atacagcttt tgtcatttgg acgacaacac catggacatt gccagcaaac
    45901 caaggtattg ctgtaaatcc aaaatttacg tatgtattgg tggaagccga tggccgtaag
    45961 tttgtcgtag cgaaagattt gctagaaaca gtccaatcag aaatcggttg ggaaaatgta
    46021 acagttctaa aagaatttgc tggacaagaa atggaatata tgactgcaag tcatccattc
    46081 tatgaacgtg aatcacttgt gattttaggc gatcatgtta ctttagacgc tggtactgga
    46141 ttagtccata ctgctccagg acatggggaa gatgactata tcgctggtag caaatataaa
    46201 ctatctgtag tgtcacctgt agactcaaaa ggattgttta cagaagaagc acctggattt
    46261 gaaggtatct tttatgacaa agcaaatcct atgatcactg atctattaaa agaaaaagga
    46321 gctttgctga agctagattt ctttactcat agttatcctc acgattggcg gacgaaaaag
    46381 cctgtgatct atcgggcaac tccacaatgg tttgcatcta tcgataaatt ccgtcagaat
    46441 attttggatg aagtagaaaa agtagagtgg gttatcccat ggggtaaaac acgtttgtat
    46501 aatatgatcc gtgaccgcgg cgattgggta atttcccgtc agcgtgcatg gggagtacca
    46561 cttccgattt tctatgctga aaatggagaa gcaatcatca caccagaaac aatcgaacat
    46621 gtggcaaaat tatttgctga acatggatca attatctggt ttgaaaaaga agcgaaagaa
    46681 cttcttcctg aaggatttac ccacccaggt tctcctaatg gcgaatttac aaaagaaaaa
    46741 gatatcatgg atgtctggtt tgattctggt tcttctcatg aagctgtttt acgtcagcga
    46801 ccagaattat cattcccagc agatatgtat ttagaaggat cagaccaata tcgtggctgg
    46861 ttcaactcaa gtatcacgac tagtgtggct atcaatggtg tagcaccata caaatcagtt
    46921 atttctcaag gatttgcttt agatggtgaa ggccgtaaga tgagtaaatc tttaggaaac
    46981 atcattgcac cagaaaaagt gatcaaacaa tttggtgcag atatccttcg cttatgggta
    47041 tctagtgtag attatgaagc agatgttcgt gtttctatgg atatcttaag tcaagtggca
    47101 gaagtatatc gtaaaatcag aaatacgatg cgtttcttat tagcgaatac agaagatttc
    47161 gatcctaaaa aagatactgt ttcgtacaat gatttacgtt ctgtagacaa atatatgacc
    47221 gttcgattga accaagtaat caaagaaatc cgagaagaag ggtacgacaa atataacttc
    47281 atgcatattt accgtacagt tatgaacttc ttgacggttg atctatcgtc attctacctg
    47341 gattttgcta aagatgtagt ttatattgaa gcagaagatg attatcaacg tcgttgcatg
    47401 caaactgttt tctatcaaac agctgtagct ttgacaaaat tattgactcc tatcattccg
    47461 catacagcag aagaaatctg gtcattcctg aaagaagaag aggagtacgt tcagttaagc
    47521 gagtttcctg gttatgaaga gtttgcaaac caagaagaat tgatggatac ctggactgct
    47581 ttcatggact tccgtgataa tgtcttaaaa gcattggaag aagcaagaaa cagcaaattg
    47641 attggtaaat ctttggaagc aaaactaact gtttatccaa aacaacaagt tcgtgaaatg
    47701 ttaaaagctt tggatgcaga tattgcacaa ttattgatcg tttcaccaga ctactttgaa
    47761 gttatggcag cagatgaaca agtgccagat aacgctatgg tatttgatga tgttgctatt
    47821 acagtggaaa aagcagacgg ggaaacttgc gatcgctgtc gccaagttcg taaagatgta
    47881 ggagtagatg aaaaacttcc tcatctatgc ggtcgttgtg caaaaatcgt tgaagacttc
    47941 tatcctgaag ctgtagctga aggattcgaa gaaaagtaaa agggaaaagg gggtgtgaca
    48001 tgttctttgt cacaccccct tttgttatca atcgtttaat tttccacgtt cacgatacca
    48061 gttatctggt tgccagatcc attcgaatcc gtctttttca agtagttcga aagctgcttt
    48121 aggtcccatt gatccagctt tgtagttggg aaaatccggt tgtgtttggt cccaagcttt
    48181 tcgaatgaca tccacgatat gccaagattg agcgacttct tcccaatgag agaagttcgt
    48241 tccatcacca ttcaacgcat ctaatagtaa tttttcgtaa gcttctgggc tattttcaac
    48301 catttctgca ctatttctaa attccaattt gattggttcc gtttcaaatc cttgccctac
    48361 ttctttccca ttcaaggata aagaaaatcc ttcagttggc tgaatataga tcgttagaac
    48421 attaggtgga agtgtcgtat cgtcacatgg ttcatcgaca gatgttttga atacattaac
    48481 aggaacttgt ttgaatacga tgttgatccg agtccctttt tcagtcaaac gtttgcctgt
    48541 acgtacatag aaaggaacac ctgaccaacg gaagttatcg atagtgaatt tgcctgcgac
    48601 gaatgtttca gtctctgatt gagcatccac tttgtcttct tcgcgatagc ccttgaattg
    48661 tacaccatcc aattcacctt gagcatattg cccgcgaacg aagttttcta acacttcttc
    48721 ttcgttataa atgcggatag ctttcaatgc tttgatcttt tctgtacgga tttctttttc
    48781 ggaaaatgct actggcggtt ccattgcaag taatgctaca acctgtaaaa tatgattttg
    48841 caccatgtct ttaagtgcgc cactatgatc gtagtagccc ccgcgatctt ctacaccgag
    48901 gttttcgcca aaagtaattt ggatattgtc gatatagcga ttgttccatt gagcctcaaa
    48961 aatattattg gcaaaacgga tagctgagat gttttggatc atttcttttc ctaaataatg
    49021 atcaatcctg aaaatatctt gttctgggaa tacttctctg atttgctggt tcaattcaaa
    49081 agcagactgg taatcagaac cgaacggttt ttctatgatc aaacgattga agccattttt
    49141 tgtcataata ttttgtgatt tcaaatgaga aacgatcgtt ccaaaaaatt gaggtgccat
    49201 agccaaataa tagacacggt taccgtttaa ttgatagtgt ccatctaatc ggtcagacaa
    49261 ttcttttaat gtctgataat gcgttgaatc atttacatca tgggattgat agtaaaaatg
    49321 gcttgcaaag gtagttgctt cttcatctga aggattcaat gcacggattg tttctttgac
    49381 aacttcacgg tagtgctcat cgctccaggg acggcgagcg gttccgatta cagcaaaatg
    49441 ctcgcctaaa tttccttttc tgtaaaggcg gaacaaagac gggtacaatt tacgctgggc
    49501 taggtcacct gtaccgccga atatcgtaaa caatacattt ttttcattag acatgtggat
    49561 acactccttt atattttatc gacaaaaatc gtgctggccg ccttaaagct gacggggatt
    49621 ttctttctat cattttggat agtgatcggt ccttcataag ctgcgatgtc aacgatctca
    49681 tattcttcgt tgatattcag gtcaatagtg actaagtaat ccaacaattc tttttcatca
    49741 agcactcgtg cgatacgcac ttttgtaccg acaggatagg actccaatgt ttgtcggcgt
    49801 tcttcatgga caacttcctc ttttttagga atcgatcctc catgaggaca atgttcagga
    49861 tgattcaaat agttttctaa atgatctgca agatgttccg aagtcacatg ttccaatact
    49921 tcagcatcat catggacttc attccagcta tagtctaaat gttcaactaa aaaaacttcc
    49981 cataagcgat gtttgcgaat cagactgcta gctttttgca gtccgcttgc agtcaattgt
    50041 acaccttgat aaggggaatg ttctacaagc tcttctttca caagtttaga aatcatttca
    50101 ctgacagatg cagctgagac atctagacta gatacgattt gtttattgtt gactttcgtt
    50161 gtatctccac ctagttctaa aatgattttc aaataatctt ctcgatttgg tgtcatattc
    50221 gatcatcctt tcatggtaaa aaaccatcat tagtaagtgt aacaaaaaaa acgttaattg
    50281 actatttttc taaataaaaa acaaaggatt tttagacgtt tttacaacaa aaaacgaaag
    50341 atgatagaga agactcattc tttcgtttat aaaggatata tatatacatt gggagggatc
    50401 tatttttcag ataacaactt atatgaagtg tctgcagatt cgtcataaag agtttgttcg
    50461 ggataagaaa agataagata aaaaccatac tgatcacgta cgactaaacg atattcatct
    50521 atttctacca cagatgtgcc aagagactga ccgttgatca aaaacgtgta atcagatgta
    50581 atctgtaatt gttgtttttg ctggctatca ctaaagaacc atgtaccttg atactttgag
    50641 agtccttctg acttttccgc ttgttttttc tttttcaagt aaataaaact tgagagaacg
    50701 gtaatgagtg aaccaagaaa atagatcatt ttttttatca ccaaatcact cctttatgag
    50761 aaaataatcg catagacata ttctttcttc tttatttcat tgtaccgaat aaaagctaaa
    50821 atttctctaa gactttcgac tggttttttt actgtttttc tacaaattat gataggatac
    50881 atagtaggaa gaaaggtgac ataatttgaa gtatcagcaa gtggaaatag ctttttttat
    50941 tgaccgcccg aatcggttta tagcacattg tatgaaaaaa gacggagaaa taattattgc
    51001 gcatgtcaaa aatacaggta gaggaaaaga ggtcctgata ccaggcgctg aagtggctat
    51061 tgtatttgct cctggaccta aacggaaaac cgcctatgat ctgattgctg taaaaaagaa
    51121 gggtgactgg tttaatatcg atagtcaatt gcctaatcgg ctggcaattg atggtatttt
    51181 agacggcacg atccttttac caggattaac tggtccattt acggtataca agcgtgaagt
    51241 aacttatctt cactccaaat ttgatattta tttagagacg gtggtcggac aaaaagcatt
    51301 tgttgaagtc aaaggtatga cacttgaaaa taaagcgatt ggtgcttttc ctgatgcgcc
    51361 cacactcaga ggattgaaac atataggaga attaacgtat gccgcacaag atggttatgc
    51421 cgcttatgtc ttgtttattg ctcagttcga acatcttcat ctggcaacga tccatgaaga
    51481 aatgcaacca gcactagctg atatggttcg acatgcgcag caaagcggag ttcaaatcct
    51541 cgcctataat tgtcaagtat cggcagatga ggtaactgtc aagcaggcca ttccatttga
    51601 tgtagatatc ccttttgaag atcctataca atagaaagaa ggtaaaataa tgatcaactt
    51661 aggtattatc ggaacaaatt ggattactca tcaatttgtc caagcagctt taagcacaaa
    51721 aaaatatgaa ttgacagcgg tatattctag acatttggaa aaagcacagg aatttggttc
    51781 tcattatgaa ggggatatcg aattcgcaac agatttgaag acttttttca atattactca
    51841 tatggacacc atttatattg cttctccgaa tagtcttcac tttagtcaag ctaagcaagc
    51901 aattttggca ggaaaaaatg tgatagttga aaagccagct ttttcgacgc caaaagaaat
    51961 ggatgaaatc atccatttgg caaatgaaca caatgtgtat ttttttgaag cagcaagaaa
    52021 tatccatgaa tcaggtttta aaaagattgc cgattactta ccattaaaag acgagataat
    52081 cggtgcaaat ttcagctata tgaaatattc ttcgcgctat gaccaagtat tagatggaga
    52141 agaaccgaat atcttctcga ctcatttctc aggtggtgca ttgatggatt taggtgttta
    52201 ccttgtatat gcagctgttg cttggtttgg aatgccaaaa gaagtccatt atttcccaag
    52261 aaagatttct actggtgtag atggtcttgg atgggggatc ttacgttatg agaattttga
    52321 tgtatcgatc cagccaggta aaatcggtga ttcttattta ccgtcagaga tttattttga
    52381 ctcaggaacg ctagtgatga atggagtcaa tgcaattgaa caagcacaat ttcatgatcg
    52441 cgcgcataaa gaaatcattg atttggcggt aacagctgca gaaaatccaa tggcagatga
    52501 agcagaggat tttgccaatg tcatggctca tccgaaagat ccagcttggg gattgttgta
    52561 tgaagagtgg atcgagctag ctagaaatgt gaaccaagtg atttatgatt taagaaaaaa
    52621 tggcggcatc aaattcgatg cagataatga ggaagatttt taatgaacga atttgtacaa
    52681 gtatttcccg aaaattggca gaaaaaatat gaggataaag ggtttatctc accttctctt
    52741 attcaacgag cagtttttca gccactaagt aataaacaaa gtatagtagc ggtttctcct
    52801 acaggttcag gtaaaacact ggcttatctt tggcctctac tactaaacgt ggaaccaggt
    52861 gaagcaagtg cgttggtcat ttttgcttct tctcaagaac tagctattca agtagcagat
    52921 gttgcacgtg aatggggaaa agacaaagag ttgaaagtcc aatctttggt gggcggagcc
    52981 aacgtcaaac gtcagattga gggtttgaag gaaaaaccag aaatcttgat tggtacacca
    53041 ggaagaatat tggaattgat gaaagcaaaa aaaataaaag cacatcaagt gaaaacaatg
    53101 gtatttgatg aagcagacca gctatttgac gctgggaaca gccaaatcat tgatcaaatt
    53161 cttcatcaag ctccaacaga gtatcagcta gctttttttt cagcgacagc agatcgttcc
    53221 cttgaaagca ttgaaaaaat cactgggaaa acgtttgaga caattgacgt gacagcagag
    53281 gacgattctc gaaaaggact gagacattac tttttacgtg taccgatacg aaagaaagat
    53341 gactacttga gacgtctcac gcatatagaa gattttcatg gtctgatttt ctttaatcaa
    53401 ctgactgaat taggcgtaat ggaagaaaaa ttgctgtata gaggtgtacc ggtaggaagt
    53461 ctggcctctg atcagaacaa gctactccga aaagcagcgc tagaacaatt caaaaagaaa
    53521 atggtcagag cacttttgac cacagatgtg gcggctagag gtttagatat tacaggtctt
    53581 ccttatgtag tgaatgcaga agttccgtta agtgaagaag cttatttgca tcgttcggga
    53641 agaactggtc gaatgggtaa tgaaggaata gtaattacac tcgtacaaga taataatcta
    53701 agagatttaa aacggctttt acgtccatca aatataacac ttgaagagat ttttttatac
    53761 ggaggtaagc tgcaaactga gcagccaacc aaagaagatg cagctcctgc agtaaataaa
    53821 aagtatgcga aaactttttc tggaaaggag cagaacccag aatataacaa agaaaaaata
    53881 agaaaaaaag ttaaaaataa atcaaaaaaa gagagaaata aaggagcaag gcgcaaataa
    53941 gcgccttttt atgctatgat tatgaaaacg ggaacaaaat tggttgccag aggaggggtt
    54001 atgtgaaaga acgtcaagaa gagatcttgc agctattgac agaaaaccaa caaggattga
    54061 cagcaagtga tgtagctgag cgcttaacta tcgatcgaag caatgcaagc cgatatttaa
    54121 gtgaactata caaggcccat catattgtaa aaaccgctgg ccgaccagtc gtttactctt
    54181 tgccaacgga aaagtcaaaa tctgatgaag tgcatgtcga ctcttctacg caagtgactt
    54241 ttgaaacact cgttggagaa aatgattctt taaaagtcag catacagcag gcaaaagctg
    54301 cgattcttta tccaccgcgg gggttgcata cgattatttt tggagaaact ggtacaggga
    54361 agtcgatgtt tgctgaatgt atgtatcatt ttgcaatcga ttcagagatg ctttctgcag
    54421 atgcgccatt tgtttctttc aattgtgcgg attatgctca aaatccgcaa ctgctctttg
    54481 gacacatctt tgggatcaaa aaaggggctt acacaggagc agctcaagat agtcccggtc
    54541 taatagccaa agcagacggc ggtatattgt ttttagatga gatccaccgt ttaccgccgg
    54601 aagggcagga aatgctgttt actttcattg ataaaggtat ttaccgtccg cttggagaaa
    54661 gtgcacaagt acatgaagca tctgttcaga tcattggggc gacgactgaa tcttcagaaa
    54721 gtttcttatc tacgttcaat cgacggattc ctatggcaat caccttgccg aatcttgctt
    54781 cacgttcact ggatgagcgt tatgaaatca tctctctttt tatcaaacaa gaagcgaatc
    54841 gtttgaatca acgtatcgat atcgaaaaag aagcgattct tgccttcatg ctttatgatg
    54901 cggaaggaaa tatcggtcaa gtgaaacgag acttgaaact agtctgtgca aaatcttttt
    54961 tacattaccg tactcatcat gagaaaaagc taatcattcg aaaagaagaa ctttcccttc
    55021 aagtacaaaa aggattactg aaaatcaagg aaataccgga acgtctcgac cgattcatgg
    55081 acagcaaaag tcagtatttg acatttgaac caggatttgc tgatgttgtc tggtcacaag
    55141 atccagaacg aaacatgcag gtctataacg atattgagga aaaagtcact tctctttccg
    55201 aaaccggtgt ggagaacatt gatctagaga ccctcatttc aaaagatgtg gatgcttatt
    55261 tccagacata tgtcaaagaa ttgactaaaa gttcgattcc caaagatctt ttgccagatg
    55321 aaatctggcg gttaaccaat caattgtatg atgtggctga aaaacggtta gaccgtattt
    55381 ataatgaaaa agctcgtttt gcttttgctt tgcatctgca aagtacattg gatcgtgtga
    55441 gagaaggtca tatgatcgtc cacccagatt tgaataacgt acggaaaaat tttaagaggg
    55501 aattccaggt agcaatcgat ctttcaacga tcattgaaga agaagaacaa gttgagattc
    55561 cttttgatga aatcggcttt attagtatgt ttctttctat caatctagga gaaagtgagc
    55621 ctgttaaaga aaacaaagta gatgttctca tattgatgca cggcagacag actgcttcaa
    55681 gcatgttgga gatggcacaa gaactattag gaacaaaagt agggaaagct tttaatatgc
    55741 cactggttat ggaagttcaa acaatgtatg aagaagtgtt acaatatgtc aaaaactatc
    55801 aagagcattt gacgaatggc ctactcttgt taacagatat gggatcatta aacaccttca
    55861 gtgatttgat ttatgaagag actggcattc ggacgaaagc tatcacgatg acaagtacca
    55921 tgattgtgat agaagcgata cggatggcaa gtgttggaag aagcttagaa gatatttatc
    55981 aaaatatcca gctttccttt gaatcgatcg tacgtgatca gttcaaagcg gaaaagtctg
    56041 aaactttaca gcgtaaaaaa gccgtaatcg tgacatgctt tactggtgaa ggagtagctg
    56101 ccaaattata tcagcgtatt atgccagtga ttaatcaaaa taaggtagaa ctgatccaga
    56161 tgcagttttt agaacgtgaa tcattcaaaa aacacatcga tgccttattg gaagaatatg
    56221 agatcaaagc aattgcggga acagttgatg ttgattatca aaatatccca tttttttcag
    56281 cctatgacgt ttttgatgat gaaaaactaa atattttaaa gcgtatagct gatgatgaga
    56341 tcccgacagc taaaattgtc gattcacttg ctggatcatt aagcgatgtc ttttctattg
    56401 atgaactgat ccgttcattg caaaagacag ttcagcagat ccagaccgat ttgtcgatta
    56461 tcgtggaacc tggagtagat actggaatcg ttatccattt agccttcatg ttagacggat
    56521 tgatcaaaca agaaagccat cggcaatttg agaaactgtc tattttctca aaaatttatc
    56581 gtttggaaat ggatatcgtt cgggcaaact tgatggcact agaacgaaaa tatcaggtaa
    56641 aaattccaga agatgatatc gcatttctta cacaaatgtt tcttgaaaat aaaataaaaa
    56701 aatgaacaca ctaatcatac tgtttacaca ctaaacatct gtgtgcgcac tataaaaaag
    56761 agctaaaaag catgaaaacg ctatttcagc tctttttttt attggcatgc ttcttgcgtt
    56821 atataaaagt gtaaaaaaat aaaaggagtg ttaaaaatgg caaaaaaaac aatcatgtta
    56881 gtttgttcag cagggatgag tacgagttta ttggtcacaa aaatgcaaaa agcagcagaa
    56941 gaaaaaggga tggaagccga tatttttgcg gtctctgcct cagatgcaga taataattta
    57001 gaatctaaga atgtcgatgt attattatta ggtcctcaag tacgtttcat gaaagctcaa
    57061 tttgaacaaa aacttgcgcc taaaggtatt ccattagatg tcatcaatat gcaagactat
    57121 ggcatgatga acggagaaaa agttcttgca caagcagaaa atctaatgaa ataaatcaag
    57181 tatgcagttg ggggtataac aacgtggaag atcaacaaaa tctagaagcc atcatggggt
    57241 taatcatgtt tggcggtaat gcaaagagcg atgcaatgga agcgattgcg gctgctaaaa
    57301 tcggtgattt tgagttagcg gataggaaaa tcgctgatgc agaagaatca cttatacaag
    57361 cgcatcatgc acaaacagac atgttgacac aagaagcaca aggaaatcat atgcaagtga
    57421 cattattgac tgttcacagt caagatcact taatgacatc tattgcgttt actgatttag
    57481 caaaagaaat catcgaactt tatcgtcgcg tatacagcga ataaatacaa cttctttttt
    57541 gaacggaaga agaaaaattt ttgaacggat tttttatttt gttataagga gaactctctt
    57601 tatataaaaa acttatagga ggaagtaaaa aatgaacgga ctatcaagtt ggatggagaa
    57661 atacatcctg ccggttgccg caaaaatcgg agcgcaaaaa catttagttg ctttgcgtga
    57721 tgcttttatc ggcacactgc ccgcaacaat ggcgggatca gtcgctgtaa tgatcaatgc
    57781 aattattcgt gatttaccgc aacagtttaa cagtacctat gctgacagtt tagcagcaga
    57841 caaaggaatc tttgccgtat tgaatgtcat catcggaatc aatggttttg tctggaacgg
    57901 tacactagca atcgccggat tgatctttgc cttttcttgg gggtataatc ttgcccgagc
    57961 ttatggagta aatgatctag ctggggggat cgttggttta gctaccttga ttcaaggtat
    58021 ttcctttggt ccagcattat cagcagcatt agatgtgaat gttccttcaa atgtcgcaga
    58081 tgcaatcaat agttctgaga ttggtgcaac gattgcagat ggcacactgt cagttggatt
    58141 atggggttgg ctaaaactag atcatttgaa tggaaacgct tattttacag ttatgatcat
    58201 gggagctatt tcagtcatta ttttttgtaa attgatgctt gccaacatta cgatcaaaat
    58261 gcctgattca gtaccaccag cagtatctaa agcatttgca gccattttac cagcgacgat
    58321 cgctttatac gtcattgcta ttatcaatta tatcgtaggt cgtgtagctg atgggcaact
    58381 gatcattgat ttggtgcaaa aatatattgc agaaccattc ctaggattat ctcaagggtt
    58441 gggagcagta ttgatcgtca cagtatttgt tcaagtattc tggttctttg gtattcatgg
    58501 accaaatgta ttggcaccag tgctagaggg aatctgggga caagcacaac taatcaatat
    58561 tgatattttc caaaaaggtt ataacggaaa aactggtaca ccagccgtgt tagatgcaat
    58621 cgaagatgga aaagcttata tgtgggtccg cggatcgttc gatgcttttg catggtttgg
    58681 cggatcaggc ggaacgatcg tcttactgat agctatcttg attttctcta aacgtgcaga
    58741 ttatctaaca gtagctaaat tatccattgg gcctggtatc ttcaatatca acgaaccagt
    58801 gatgtttggt ctgccaatcg tattgaatgc aatcatgttt atcccattct tgattgctcc
    58861 agtagtagca acagcaattg gatggacagc tacttatctt ggcctagtcg ctccggtatc
    58921 gcagcaagtc acatgggtag taccgccgtt cttattgtca ttcttagcaa caggagcgga
    58981 ttggcgggca ccgatcgtaa cattagtatg tatggtcgtg actttcttga tttgggcacc
    59041 atttgtcatc tctgcaaaca aaatggaccc tgccgcaggc gaacaacaat aagatgaacg
    59101 tgaggagcgg gacgctttaa gtttcgttcc tcataatgaa ttttcgagga ggaactgaaa
    59161 tgattacaat tgccagtgca atgcaacaag atgaaatcaa agaattgtta gaaggataca
    59221 atgaagaagg aatgactttt acatttaaag aaaaacaagg aatcaagctg ttctttgaaa
    59281 caagtgcaga agatccggaa gttgcggcta aaaaagcaaa ggaactaatc aaagcacagc
    59341 cttggggaac agtcctttat tttcaatcaa cagctgttta gaaaatggtt taaaaagata
    59401 ggatggttga gatgataaaa aacggtttat ttttatgtag tattggttct ttgatcgtgt
    59461 tgtatagttt gaatccacat tcgcaaaggt atgatttgta tagtatggtc acaggtcttt
    59521 ttctaacagg attaggaatc tatttcttca tgaaggggaa aaaaagagaa gaagcaaagg
    59581 agaaaaataa ctaatggaaa tcacgaaaaa attaaatatt ccagcatcgt ttttttacga
    59641 taaagttatg gactctgtcc ttttcgatgt acgtaaagcg accggcaaat cagtaactag
    59701 aaaacagctg aaaaatttgg agtacgtcaa acaattctcg caaaataacc gggctcgcat
    59761 aaaaatcgaa gaagtcattg aaaaccagtc ttataagttt cgtacatcga caacgaagaa
    59821 tgaattcgtc gttcattatc aaatcaaacc attggacgat tcgacctgtg aggttcatta
    59881 cacagaaaaa atggagtctt atggcatgct tcaaaagttg aatgatatgc ttttaggaac
    59941 aatcttgatg tattttaaac ggagacgttt caaaaaaatg ctagatatga tcgaagcaac
    60001 gtattaaatc aatgaaacat ggtactattt agttattgaa aaatgaagta agctagaagg
    60061 aatttttcat aggaagatag atctttcctg cgaatcctca ctagcttttt tgtaggaagg
    60121 ggcagcaaaa tgacaaacaa agtaagagag ctaggtgtat cttcgattga ggaaatgttt
    60181 gatttagcca cttatgcatt taatggggca gatacacctg aaaggcgaga acgttttcgt
    60241 acacttgcgg agaattcttg gaattacgga ttttttgatg aggaggatcg gctaacaagt
    60301 caagtcatgg cgaccccttt tccagtgaat ttttacggac aagagtacct aatggccggc
    60361 attggctatg ttgcttctta tccagaagca aggggacaag gcgggatcaa tcagatcatg
    60421 gaaaagatcc ttgaagattg ccgtgatcgt aaagtaagct tatcttatct ggcgccattc
    60481 tcctatccct tctatcggcg ctatggctat gaacaatcat ttgataaaat cagttatcaa
    60541 ctaaacagtc gtgatgtgcc gtttatcaaa aaaaaatctg gaaaaataaa gcgtatggat
    60601 tttgaagatg caaaagctgc aatcagaaga atttatgaac agatgccgga aaaccaacga
    60661 gctggtctga agcgcgaaga ctggtggtat acgtataaat tcaagcaaaa aggcaattac
    60721 cagtttgctt tgcattttga tgataatgag gaagcaacgg gatatatcgt ttatcagctt
    60781 gcaacgtctt cctttattat tgcggagtgg ggatttttgg atcatgatgc tttttgcagc
    60841 cttgttcgct tcgtaagttc tcacaatggt gcttttgaaa catttgaata ttcatgcggc
    60901 tacggcggta atgatcaaaa ttatctatta gaaaatccat ttgcttctgt aatgatcaca
    60961 ccttatatga tggcgaggat cgtagatatt tctttgtttt tggaaaatta tccatttaaa
    61021 aagagacaag ctgcatttgc cctacaaata gaagaagatc attatgcgaa atggaatgaa
    61081 ggaatcgtgg aagtaacaat cgaaaatgga aaaaatcatc tctcaaaagt agaacaaaca
    61141 aaactcccaa tcgttcgagg aagtatccaa catatcacgc agctattatt agggtatcgt
    61201 aaagtggaag agttggtatt ccaagaaaga atacaggtga caaaagaata tgaagaaacg
    61261 ttaagctcaa tcttgccaca gcaacagcca attcttaatg actatttttg ataaaaaacg
    61321 ggtgatgaca atatttttgt caccaccctt ttgtttttca tttggcattt gaaggaaaat
    61381 gttaaagtaa atcctctttt ttcgtaaatt tgaatttttt gttttctttt gttttagggt
    61441 tttctttttt gtggtcttgg attgcttctt tcgtaatctt ttcccgattt tttttagatg
    61501 aatctaagcg ttctgcgtat actttttctt ttggcttatc attcattgga aaggacctcc
    61561 ctacattatt tcattctacc tctagtttat catctgttaa ggactagtgg aaacgttttg
    61621 cctaacttat aagctgattt ttttagcaga taagcttgca agtaaaaaac aaacaagcta
    61681 caattgattt attgaaattg ttcctgtagc tcagtaggat agagcaatcg cctcctaagc
    61741 gataggtcgc tggttcaatt ccagtcagga acgtataaat aacccctgta tcctttgcgg
    61801 tacaggggtt tgttttgtat ttgtgccaaa aatgtgccaa actagttatc atttttatat
    61861 aaactatcga acacagtatt gattttttct ttatcctgat cttcaagctc tcgaacaata
    61921 tgaagataag tagacattgt agtttctaaa ctgctatgtc ctaaacgttt cgatacgcta
    61981 agtatattta ctccttgata aagtaaaata gatgcatgtg tatgccttag tccatgcaaa
    62041 gtaaattgct tttcaatccc taattctttt aattttttgc gcaaatattt gctgacggcg
    62101 tttgatgaaa ctaatccatt ttttaagttg aaaaaaacaa aattatgagg gtttttgatt
    62161 tcaaaattct cgaataattc tttttgattg attttaaact tttttaagag atcaatcgta
    62221 tgtttatcaa tggaaatttt cctatttgaa gtttcgtttt ttgtttttcc ccattcattt
    62281 aatttataat tccatgtttt attaatagtg atgatttgat cttcgaaatc gatatcttcc
    62341 catgttaatc ctagaagttc agcaaatcgc attccagtgg caccagcaac taacacgagc
    62401 ataggagaag agtactgcgc agaaaggttt tcttctacga gtttcattaa agatttaaat
    62461 tgatcataat ctaaatattt atcttctttt ggcttcaatg aatcttttcc tttgattact
    62521 gcttttctag ttggatcaaa cggaattaat ccttcttcta cggcatcttt caacgacgct
    62581 ctaatatggt tattaaactt aataacggta gattttacat ggtctttagc atacttattt
    62641 aaaaagcgtt gatatcccac tctatctaaa tcagaaatca ataccgctgg catatatttc
    62701 tttatgttca ttaacgtatc ttcgtatttt ctataagtga taggagatac tgttccttct
    62761 ttataaagtt gcatccaatc ctcaaagtaa tctgataata atagattttt gcgatcccct
    62821 ttaaggcctt tgttcaagtt atattctaat tcgttggcgg catcttttgc ttctcctttt
    62881 gtcttaaatc ctgattttct tatcttcgtg tattttccgt catctttttt gtatgaaatt
    62941 tcgtattgcc aactatttcc acgtttgact aatcttgcca taattgaatc agctctcttt
    63001 ctctgataca ataggaacta taaagaagcc cattgtttgg gttttattat tttttgcaca
    63061 tcctttttgc ttgccggcgc aaggatgtgc tatttgtcgt tttaatggta taattggatg
    63121 tgtattagat catccaatca gaggtgttta ttatgattaa agtgattttt aaaagaataa
    63181 ctgaacttcc agagtttaga ttatttgttg attattggta tgtctggttt tttattgttg
    63241 cattgctgat aggtctagat gtcatattag aaagaaaact taagtaagag ttgagttaat
    63301 aaattgaatt cctaaatgag atattttgta gcccatattt tgtttgccga catcacctaa
    63361 tactatggag ctaatcgttt tttctaacag accaaaatca gataatctat ttaagctacc
    63421 atctattagc tcttgtgaat agccagacat gctgatattt ccagtgaaat tttcgtttcc
    63481 acgactcatt aagcatgcta acaattgaat ctcaatcgat gttaattgat tcagtacaag
    63541 aagataatac tcctcgttat cccatgaagc gttgttaatt cgaagtaaac aattcttata
    63601 aagattttta tacatatcta ttttattttg tgcctttgat ttttctacct cgtcatttat
    63661 cccttcgaat attccaatga gttggtcctt gttctctaag ttgaaaacat cttctggaat
    63721 ttgatcgcga atgttttcta atgattcgtt taaatttttg tagaatttct cgatgcgttt
    63781 gaaacgcttc tcgtttttag acccaaaata aagtgactgc acaacgccac caatataagg
    63841 aattgctcca ataccgcctt caactattaa ttcaagatat tcttttttgg ttagtttttc
    63901 attgctatta tccatagtta cctcctattt tgtcatttcc tttacaagca ttgtgactcc
    63961 tgatggcact cttttgtata ggttttatta ttgtgcacaa tctaactctt agtggggcgt
    64021 ggttcattta ttttttactt actttactat taatcatatc ccattgagca taggattttt
    64081 ctatgttcga tcctcttgaa ctaatttctc ctgtaaaagt tactttatcg ccctttgtaa
    64141 aggtatcagc attgtttaag tccttcgcaa aaattacata tgaaacttta ggtgttgcag
    64201 aaatatcatt ccaactttta tcatcgtact ctttgtctgc tataatggct attctagtag
    64261 tcattgattc gataacagtt ccttcaaaag tatatgtttt accactaata tatttagtaa
    64321 aagctgtatt ttgctcagta gctggagtaa tagaataata actatctaag aaagcacccc
    64381 agtcatgatt atttgtctct aaaaattctt caaattgagt taggctttta ttgctgctgt
    64441 tagatgtagt agtttgagta tcagatgatg agctttcctg tttgttattg tcatttgcct
    64501 cgtttgatga ggaaagcgag aaacctacgg tagtaaaaat aatcccgata ataaggagaa
    64561 tcataggtgt tttcttattt gtttttttga taaatcttac aattaacatg attaaaccta
    64621 taataaataa aacaatacca gtaaaaccaa atataccgtc cattaataga tcctctttct
    64681 cttttgatat tgaatattat aaaccaagca attctttttt cttaaaatcg aattcttctt
    64741 gattaataat cccttcatct aacaactctt tatacttttt aatttcatct gcacttgaaa
    64801 tttgtgtttc tttggaatta ttatctaata tagattgttg cttctctata tattttttta
    64861 aattttccat ttcattccaa tatttttttg taaacataac ggtgttttca tctttcgtgg
    64921 ccgctaatat acctccgcga ctttcgtttc cgcctagcaa ggtaaattgt atataaccat
    64981 tactcatacc tggttttttt aattgtacgg ctgatatatt tttaaagggg atagtttttt
    65041 ctcctttaat accttgatta actaaattta taaatccttt tctaataatt gaaatagaat
    65101 catcagttac tttaatcaag gtttttcctg gagattttat taaaatctct ttttccattt
    65161 tttcttcctc atttctgtga tatgattttt tttgtagaag ctcatatatg aggaaagagc
    65221 ccgtgttgca gcacggg
//
LOCUS       DACCKU010000004        60076 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.004, whole genome shotgun sequence.
ACCESSION   DACCKU010000004 DACCKU010000000
VERSION     DACCKU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 60076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60076
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.004"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(56..1135)
                     /gene="pepA"
                     /locus_tag="IVN13_000227"
     CDS             complement(56..1135)
                     /gene="pepA"
                     /locus_tag="IVN13_000227"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP7010749.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            1335..1685
                     /locus_tag="IVN13_000228"
     CDS             1335..1685
                     /locus_tag="IVN13_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7010750.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            1908..2855
                     /locus_tag="IVN13_000229"
     CDS             1908..2855
                     /locus_tag="IVN13_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7010751.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(2912..4330)
                     /gene="pepV"
                     /locus_tag="IVN13_000230"
     CDS             complement(2912..4330)
                     /gene="pepV"
                     /locus_tag="IVN13_000230"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP7010752.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            4479..5324
                     /locus_tag="IVN13_000231"
     CDS             4479..5324
                     /locus_tag="IVN13_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP7010753.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            5328..5768
                     /locus_tag="IVN13_000232"
     CDS             5328..5768
                     /locus_tag="IVN13_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP7010754.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            5818..6012
                     /locus_tag="IVN13_000233"
     CDS             5818..6012
                     /locus_tag="IVN13_000233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010755.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            6148..6834
                     /locus_tag="IVN13_000234"
     CDS             6148..6834
                     /locus_tag="IVN13_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7010756.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(7241..7930)
                     /locus_tag="IVN13_000235"
     CDS             complement(7241..7930)
                     /locus_tag="IVN13_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010757.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(7905..10034)
                     /locus_tag="IVN13_000236"
     CDS             complement(7905..10034)
                     /locus_tag="IVN13_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP7010758.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     PGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            complement(10082..11419)
                     /locus_tag="IVN13_000237"
     CDS             complement(10082..11419)
                     /locus_tag="IVN13_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP7010759.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGLAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            complement(11671..12030)
                     /gene="rplT"
                     /locus_tag="IVN13_000238"
     CDS             complement(11671..12030)
                     /gene="rplT"
                     /locus_tag="IVN13_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP7010760.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKDALAK"
     gene            complement(12083..12283)
                     /gene="rpmI"
                     /locus_tag="IVN13_000239"
     CDS             complement(12083..12283)
                     /gene="rpmI"
                     /locus_tag="IVN13_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP7010761.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(12355..12879)
                     /gene="infC"
                     /locus_tag="IVN13_000240"
     CDS             complement(12355..12879)
                     /gene="infC"
                     /locus_tag="IVN13_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP7010762.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            13199..13831
                     /locus_tag="IVN13_000241"
     CDS             13199..13831
                     /locus_tag="IVN13_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7010763.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(13833..14738)
                     /locus_tag="IVN13_000242"
     CDS             complement(13833..14738)
                     /locus_tag="IVN13_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7010764.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASHILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(14823..15476)
                     /locus_tag="IVN13_000243"
     CDS             complement(14823..15476)
                     /locus_tag="IVN13_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7010765.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            15798..16742
                     /gene="mvk"
                     /locus_tag="IVN13_000244"
     CDS             15798..16742
                     /gene="mvk"
                     /locus_tag="IVN13_000244"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP7010766.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            16735..17712
                     /gene="mvaD"
                     /locus_tag="IVN13_000245"
     CDS             16735..17712
                     /gene="mvaD"
                     /locus_tag="IVN13_000245"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP7010767.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            17725..18810
                     /locus_tag="IVN13_000246"
     CDS             17725..18810
                     /locus_tag="IVN13_000246"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP7010768.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            18807..19871
                     /locus_tag="IVN13_000247"
     CDS             18807..19871
                     /locus_tag="IVN13_000247"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP7010769.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLASLGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(20120..20923)
                     /locus_tag="IVN13_000248"
     CDS             complement(20120..20923)
                     /locus_tag="IVN13_000248"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP7010770.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGATIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(20943..22232)
                     /gene="kduI"
                     /locus_tag="IVN13_000249"
     CDS             complement(20943..22232)
                     /gene="kduI"
                     /locus_tag="IVN13_000249"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP7010771.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVINIGGPGFIEIDGQKEEMKKQDGYYIGKETRHVVFSSQDAENPAKF
                     YISSVPAHHKYPNVKISIDQVKPMETGEGLTLNERKIYQYIHPNVCESCQLQMGYTIL
                     EPGSAWNTMPCHTHERRMEAYVYFDYANEDTRVFHMMGKPDETKHLVVDNEQAIISPS
                     WSIHSGVGTSNYSFIWAMCGENITYTDMDMVQMDQLK"
     gene            complement(22248..22727)
                     /locus_tag="IVN13_000250"
     CDS             complement(22248..22727)
                     /locus_tag="IVN13_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP7010772.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLSGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSIPVSGILIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22733..23761)
                     /locus_tag="IVN13_000251"
     CDS             complement(22733..23761)
                     /locus_tag="IVN13_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP7010773.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDTFKNDPAYKDLY
                     QKIQEQGA"
     gene            complement(23778..24884)
                     /locus_tag="IVN13_000252"
     CDS             complement(23778..24884)
                     /locus_tag="IVN13_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7010774.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25113..27710)
                     /gene="adhE"
                     /locus_tag="IVN13_000253"
                     /gene_synonym="adhC"
     CDS             complement(25113..27710)
                     /gene="adhE"
                     /locus_tag="IVN13_000253"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP7010775.1"
                     /translation="MAKVMEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIICDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(28015..28395)
                     /gene="yajC"
                     /locus_tag="IVN13_000254"
     CDS             complement(28015..28395)
                     /gene="yajC"
                     /locus_tag="IVN13_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP7010776.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKKEK"
     gene            complement(28492..29637)
                     /gene="tgt"
                     /locus_tag="IVN13_000255"
     CDS             complement(28492..29637)
                     /gene="tgt"
                     /locus_tag="IVN13_000255"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP7010777.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            29855..30553
                     /locus_tag="IVN13_000256"
     CDS             29855..30553
                     /locus_tag="IVN13_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP7010778.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(30721..33360)
                     /locus_tag="IVN13_000257"
     CDS             complement(30721..33360)
                     /locus_tag="IVN13_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7010779.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(33979..35010)
                     /gene="queA"
                     /locus_tag="IVN13_000258"
     CDS             complement(33979..35010)
                     /gene="queA"
                     /locus_tag="IVN13_000258"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP7010780.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            35203..36378
                     /locus_tag="IVN13_000259"
     CDS             35203..36378
                     /locus_tag="IVN13_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7010781.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            36382..37023
                     /locus_tag="IVN13_000260"
     CDS             36382..37023
                     /locus_tag="IVN13_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7010782.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            37020..37937
                     /locus_tag="IVN13_000261"
     CDS             37020..37937
                     /locus_tag="IVN13_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7010783.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYDQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            37934..38605
                     /locus_tag="IVN13_000262"
     CDS             37934..38605
                     /locus_tag="IVN13_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7010784.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATDGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(39147..39635)
                     /locus_tag="IVN13_000263"
     CDS             complement(39147..39635)
                     /locus_tag="IVN13_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP7010785.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPKDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(39836..40102)
                     /locus_tag="IVN13_000264"
     CDS             complement(39836..40102)
                     /locus_tag="IVN13_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP7010786.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQSNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(40102..40365)
                     /locus_tag="IVN13_000265"
     CDS             complement(40102..40365)
                     /locus_tag="IVN13_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7010787.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSGFTNIDDIDWEAL"
     gene            40840..41025
                     /locus_tag="IVN13_000266"
     CDS             40840..41025
                     /locus_tag="IVN13_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7010788.1"
                     /translation="MALSYGNKKWSLDEAEISTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(41167..42990)
                     /locus_tag="IVN13_000267"
     CDS             complement(41167..42990)
                     /locus_tag="IVN13_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP7010789.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(43587..46292)
                     /locus_tag="IVN13_000268"
     CDS             complement(43587..46292)
                     /locus_tag="IVN13_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP7010790.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPILQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(46598..47545)
                     /locus_tag="IVN13_000269"
     CDS             complement(46598..47545)
                     /locus_tag="IVN13_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP7010791.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYHLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            47735..48004
                     /gene="rpsN"
                     /locus_tag="IVN13_000270"
     CDS             47735..48004
                     /gene="rpsN"
                     /locus_tag="IVN13_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7010792.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(48180..48542)
                     /locus_tag="IVN13_000271"
     CDS             complement(48180..48542)
                     /locus_tag="IVN13_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7010793.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(48553..49674)
                     /locus_tag="IVN13_000272"
     CDS             complement(48553..49674)
                     /locus_tag="IVN13_000272"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP7010794.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(49690..50040)
                     /locus_tag="IVN13_000273"
     CDS             complement(49690..50040)
                     /locus_tag="IVN13_000273"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP7010795.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(50170..51681)
                     /locus_tag="IVN13_000274"
     CDS             complement(50170..51681)
                     /locus_tag="IVN13_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7010796.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQSETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(52003..53355)
                     /locus_tag="IVN13_000275"
     CDS             complement(52003..53355)
                     /locus_tag="IVN13_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP7010797.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKDFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVPDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKDSLSMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(53508..54584)
                     /locus_tag="IVN13_000276"
     CDS             complement(53508..54584)
                     /locus_tag="IVN13_000276"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP7010798.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            54780..56525
                     /locus_tag="IVN13_000277"
     CDS             54780..56525
                     /locus_tag="IVN13_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP7010799.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDSVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENGTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTRASQKTETVHEKEFGSFTFRASTSTEDPVAPYTVNYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTLGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(56774..57241)
                     /locus_tag="IVN13_000278"
     CDS             complement(56774..57241)
                     /locus_tag="IVN13_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP7010800.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVIIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAIDQGLPSTEDSR"
     gene            complement(57225..58598)
                     /locus_tag="IVN13_000279"
     CDS             complement(57225..58598)
                     /locus_tag="IVN13_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010801.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKEIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLSWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDAAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFILAKEMGEPIHLLLITLNHESVSIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(58921..59409)
                     /locus_tag="IVN13_000280"
     CDS             complement(58921..59409)
                     /locus_tag="IVN13_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7010802.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELVSDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(59431..60033)
                     /locus_tag="IVN13_000281"
     CDS             complement(59431..60033)
                     /locus_tag="IVN13_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7010803.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLLFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        17301 a        12766 c        11169 g        18840 t
ORIGIN      
        1 aaaaaaaatc tggcaaaagc aatcttttgc cagattttat agtttcacta tcgcattatt
       61 ttccgtatac gatcgtattc actgttgatt tatccaaacc tttcagtgtt tgaatcaaca
      121 tttctcgtgc agcttcaaaa tcgcgaatac tgaacatcgt ttgatgcgta tggatataac
      181 gtcccactac gccaatcact gtacttggga ttccttcgtt ttgtgtatga gcagcacctg
      241 catctgtacc gccttttgaa acaaagtatt gataagggat attgtttgtt tcagcgatat
      301 ccagcaagta ctcgcgcaaa cgaggaagca tgatcaaacc tggatcttgg atacgcatca
      361 atgtaccttc tcctaaatgt ccgaatgtgc catttttcgt cactgtatca tctgctgcag
      421 agcagtctac tgcaaagaac agatctggct taaatttatt gacagaagct ttagaaccac
      481 gcaagcctac ttcttcctgg acattcgcgc ctgcaatcaa tgtatgacct aatgtttcat
      541 tttgcaacgc ttctaaagct tctaatacca ttgtacagcc atagcggttg tcccatgatt
      601 tgctgatgat attctttccg ttggctgttt tgatcgtttc agcgtaagga acgattgtgt
      661 ctcctgggag gacaccgtaa ctcattgcct cttctcttga ttcaaaccca gcatcgaaca
      721 aaatatcggt tacttctact gatttttgtc cgcttgttcc tcgcaataaa tgcgggggaa
      781 cggaagaaga aatacatgga taattcttac cagtagaggt tttaagtgta aaacgctgtg
      841 cagaaacaac gtaaggattc cagccgccta gaggtacgac tttaaaaagc ccgttttctt
      901 ggatttgtgt cagcatgaat ccgacttcat ccatatgtgc tgcgaccatt acacggggtg
      961 catccgcttc ttcatgatgg cgcagaccaa aaatgccgcc caaaccatct aactgtaact
     1021 catctaccag cggttccata tgactttcca tataagcgcg aacatcttgt tcaaaaccgc
     1081 ttgttccttg taattctgtt aactctttga ttcgttgaaa tgttttttct tccatatgct
     1141 attcgtctaa atttcagact aaactcctct cagacttata ttgatctgcg gataaaatat
     1201 gattttttct gcagaccgat cgtatttatt atagcttatt ttttctggta gcggttattt
     1261 atttcaaaga aaatatgaga gtgtggtaaa ataagcacaa ggagtaacaa gtgagaagaa
     1321 ggaggtatgt ccacatgtat gaagaaagcg aactaagcta ttttaaaggc gggttagcag
     1381 ttggaatcgg tctaggatta ttgagcgggg tagcttcaac gatttggtat catcgcaaaa
     1441 aagtattgtc agcagaccaa gtacttgaaa atatcaagtc atcttttcta aaagaaggtg
     1501 caatcgaagg ttcttggatc tcttttgaaa aggaacctat taggaaattc gccgttcggt
     1561 cgagtgctta tcggggaggt ctttctcgaa tagaagatgg cgaaactgtt tattatgaat
     1621 ttttagcaga tgctcataca ggaaccgtat tagaaatttc aagaaaaaaa ggtgcaacag
     1681 attaatacgt caaaaaagtg agagaacgaa tacgcaccca ctattcaaaa gagaggtatc
     1741 atgtccttct tttgtagtgg gtggtttttt agatagagct ttccaaacat atataccagt
     1801 attttctttt taaataaatg atatttagcg ggaaaaacta gcatttcctt tgattatgag
     1861 gtatttttaa tttagctttt gttttctata gttaaggaga gtgaaaaatg gaacaacaag
     1921 aaaaaattgc tgttctgatt ccttgttaca atgaggaagc aacaatttca acagtcattg
     1981 cagactttaa acgagaatta ccagaagcag atatttatgt ctatgataat aattcgaccg
     2041 atcgaacata cgaattggcc gttcaaggtg gagcaatcgt aaaaaaagaa cctcgtcaag
     2101 gaaaaggaaa tgtgatccgc caaatgttct ttgatatcga tgccgactat tatttaatgg
     2161 tagatggcga tgatacttat cctgcagaag ctgtacatgg tttgctcgag aaattacgtt
     2221 ctggtgaagc agatatggtc attggtgatc gcctatcaaa tggaacctac tttgatgaga
     2281 ataagagacc tttccacgat tttggaaata accttgttag aaatacaatt actagaatgt
     2341 ataaaacaaa aattcttgat gtcatgaccg gctatcgagg attcaatcgt atctttgtca
     2401 aaagttttcc tattatgagt tctggttttc aaatcgaaac ggaattgacg atccatgctt
     2461 tagataaaaa attcaagttc gttgaaatcc caatcgatta tcgtgatcgt cctgaaggca
     2521 gcgaatcaaa attgaataca ttctctgacg gattcaaagt gatcatgatg attattaaaa
     2581 tgtttaaaga ttataagcct ttgcttttct ttgggatttg gaccatcttc ttctttttat
     2641 ttggattggt cacggggatt cctgtgattc gcgaatatat gctgacaagc tttattacac
     2701 gcatcccttc tgcgatttta agtactggat tgatgacttt agcacttctt tcattagtaa
     2761 cagggttgat tttggataca gtcgtcacga acgctaagaa agaatatgaa ttgaatctat
     2821 atcatgttta tcatgaatac cacagtagaa agtaaaaaaa ggatcaagac tgcaaaaata
     2881 atttgcaatt cttgatcctc tttttaatta attatttcgc caaacgatag atggcatctg
     2941 catagattgc tgttgctctg aacaagtcat caagactcat aaattcgttt gcttgatgca
     3001 ttgtatcagt atatcctggg aacatcgctc cataagcaac gccacgtttc aacaaacgtc
     3061 cgtaagttcc gccaccgatg atttgttcgt agcctttttc tccagtgtgg tcttcataga
     3121 cttgcaataa tgtagccacc aatggatcat ccattggtac gtaatgtggt tccattacgc
     3181 gagcacctcg agtaactgtt gcgccttctt gccccactgt ttgttcgatg tcactttgca
     3241 atccgtctac tgttacacct tttggaaaac ggaaattcat attgatgaag ttcgcttcct
     3301 ctgtaccatt ttcttcaaaa gcaaatagtc cagcattcat tgtcaatttg cccatttttt
     3361 catcttcata agcgactcct agtttttctc cataaaaatc ttcatggaca taagcagctg
     3421 ctacatggat aaattgttta gcagatccta ggaattcata gttatctaag aacaatgcca
     3481 agaaagaacc tgcattgata ccagattgtg ggcttgcacc atgtgcgcct ttaccaacta
     3541 attcgatttt tacatacgta ttgtctgctt caatcgttcc gcttactgga tttgcttcga
     3601 tgaattggta aaatgcttct tccatcgcga tagctgcatc tgcacttggt acttgaagtg
     3661 ctgcagttgc tgttcctggc accatatttt cgcgaagtcc ggaaacaaaa gatttcaaga
     3721 cataatctcc tgcattatcg cctttgaatc gtaaagctaa agaaacattt cctttttcac
     3781 cattgatgat cggaaattca gcatctggtg agaaaccaaa atctggctct tcttcatgtt
     3841 cgaaataata agccatatcg ccccagccgc tttcttcatc gctgccgact acgaagcgaa
     3901 ctttttttga tagtttcaag tcaagttctt taatgatctt catcgcatag taagctgcca
     3961 tacttgggcc tttgtcatcg cttgatccgc gtgcaaagat ctttccatcc ttaattactg
     4021 gttcataagg atctgtgtcc cagccatctc ctgcaggaac gacatccatg tgtccaaata
     4081 tccctagcgt ttcgtctcct tcgccaagat caatatgccc tgcatagtta tcgacatttt
     4141 ttactttaaa tccatcacgt tcgccataag ctaacatatg tttcaatgcg tcgcgaggtc
     4201 caggtccaaa tggtgcatct ggtgttactt ttgagtcatc gcgctcgctg ttgacacgta
     4261 aaagattttt caagtcttca agcaagtctt ctttacgtgc ttctacttct ttttgccaat
     4321 caattgtcat aaagttaatc ctccacttat ttaagtcttc gtgttcatga taccacaaat
     4381 tacagaaaaa aatagttcaa aaaatctgac aattcctttt cttatctgtt tttctctaaa
     4441 aaaaatatac gctataatga gtaaaaggag gtttttcgat gctgataaac gaacaactag
     4501 tgaaaagagt catcacacct cgtccaaaaa tttcacataa aggaacattt ggacgtatcg
     4561 ttttgattgg cggcaatgaa caatatggcg gagcaatcat tatgagtgct ttagctgccg
     4621 tacacagcgg agcgggtctc acgactgtag taactgatga aaaaaatcgg agtgctcttc
     4681 acagtcatct gccagaagca atgattgtag attggacgga tactgaccag tttctttccg
     4741 taataaagca agcagatgta ctcttgattg gtcccggatt gggtacttct tctaagagtt
     4801 tggaacgttt caaacaagta ctagaaaacc aatcaaaaga acaatggctg gtgattgatg
     4861 gctctgcttt aagtttactt gctgctaatc atcttttgct accctatccc gaacaaactg
     4921 tacttacacc tcatcaaatg gagtggcaac ggttgagttc tctaccgata caagaacaac
     4981 gggataaaga aaacaaaaag aagcaacagg aaatcggtgc tgtgctcgtt ttgaaaagtc
     5041 atgagacaaa aatttatggt caccagcact tctacaccaa tcctttaggt tctgctgcta
     5101 tggcaacagg cggcatgggt gacactttag ctgggatgat tacaggattt ttagcccagt
     5161 ttcctcaaaa tgaccaaacg attgcagcag ctgtttatct tcatagtttc attggagacc
     5221 agctggcaaa agaacaatat attgttttgc cgacacaaat cagcgaagca cttccgaagt
     5281 ggatgaatta tttttgtaaa aaatcaatgg aaaacgaggc atgagtcatg gagaaattta
     5341 caggttatgt gcgagaacct ttttattatg aaacagatca aatggggatt attcatcaca
     5401 gcaattatat ccgctggttt gaagaggctc gggtagcctt attggtacat ctagggtttc
     5461 cctataaaaa aatcgaagaa tctggtatca tcatccccgt tctggaagta aattgcagct
     5521 ataaagaaat gatccattac ggagaaaaag tgagcatcca tccaactatc caaaagtata
     5581 cggggacacg gttggatttt agctacgaaa ttgtgggaac tgaagacaaa aaattaaaaa
     5641 ctactggcag tagtaaacac tgttttttac tttctgattc gcagcgatta gtgaagctga
     5701 gcaaagtcaa tccggagtta gatcagttat tccaagcgta cgcttctttg gatgatgtaa
     5761 attcttaata aagaccgtct aagcattttt cttgtattgt gtcgagtagt tcgtataatg
     5821 aaaagaaaaa agcaggtgat ccccatgtat gtcgttaaag taatgcacgg ttacatcgat
     5881 aaaactggct gtcgcactcg ggaaaaaaac ttagacaatc tgctcatttt caaagacaaa
     5941 aaagaatccg aagcttttgc taaaagaatc ggcggtcgtg tcaaaccgat ccaagaggta
     6001 cgaccagatt gattcaatat ttttcttttc gaggctctaa taggcatgcc tatcagggct
     6061 ttttttgcag actgtgtttt tatgcccaca cgatttgtaa gcgatttatg ttataataga
     6121 ttatatctgc atagaaagga aggttaaatg gttatcaata atttgcaaca gcaagcttat
     6181 gaatctatac gtaaacaaat catttattcc gagttagcac ctggaagtaa aatctccgat
     6241 aaagtgttag aagaacaact agctatcggg cgtacaccga ttcgagaagc actgatccaa
     6301 ctgcgcaatc aagagcttgt aaaaaccatt ccacagtccg gcacttatgt ttcaaaaatc
     6361 gatatccgat ctgcttcatt agcccgttat acgcgggaaa agttggaaaa accgatcctg
     6421 caagaatgca gtgcgaaaat gaccaagcaa gatcaagcta aactagaaaa aatactagaa
     6481 caaaccgatc aggcagtgat cgctcaaaat aaaaaagctt tttttgattt ggatactgat
     6541 tttcaccgaa cttgttatga aattgctgga aaacgagaaa tttgggattg gctagaaagt
     6601 tatagtacgc acttgaaccg tttcagatgg ttacgtttga cgatttcaga attagactgg
     6661 ggtcgagtat tagatgaaca tcagacaatg cttcaatcaa tgattgatca taactttgat
     6721 gaagtcggat ttctttgcac gatgcattta catatgatta tcgaggagca agaatacgtg
     6781 attcataatt accccgatta ttttgaagat attcaggaca gacccatcaa ataagaggtt
     6841 ttagaatcag cgttttgacc tgagtattag agaaagaaac ggcaaaatag ttcctcatat
     6901 tttgccgttt tttttgagct aataccgagg gtcagctgat tgcacaccgt ttattgagag
     6961 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caagtaaaaa tagcttctta
     7021 tgtttttaac ttggctggac ttattaccgc agatgcaagc ttttgaacac cgttttccaa
     7081 gaggtcgtga gcctgccgtt catctccgag tcacaaggaa caatttcaaa aaacagcttc
     7141 tcatgtttgt tgaatatttt gacttgtgac cgaggagttg acaggagaac accgtttatt
     7201 cggaaaaaag gactgtgaca cgacttttgt cacagtctct ttatttgtaa tctaaatcat
     7261 actccgtaca tcttttttct atttctttta tttcttctaa gatcccttgg ctacgaattt
     7321 cattatatgg ccaaagtaag actgcctcta atacataagg cgaaaaatcg ctttgattca
     7381 tctcttttga aaaactcatc gctgctatct cgctcaatgc acgaatgttt gattgatgtg
     7441 tataaaaagg caaccgccac agcacaattt tttcgctttg cgtataaatt cccttaacag
     7501 aagcttcgat gtggtgaaat agttcatgtc caataatcaa agcttgcgct gtttctttag
     7561 agatcgaagc tagtcctgaa cattttaatt tttctaacgg ctccgtcatc aatcggatcc
     7621 cttcatcttc tgcataagtt gcaaaagtga ttcgttcgtt tatttgctct gattcacagg
     7681 aaacgattgg aacagccatt tgttcacaaa tttctcctac atcggcaaaa gggtactttt
     7741 ttttgatgct acatgccgtt tcgataccac aatcgattga ttgttgtata agaattttct
     7801 gcttggacaa aggaatcttt ttccaaaata aatctcgccg aaactggtag aatccccaaa
     7861 tttcttcggg gatctgttgt aattcgttta gtaactcttc taacttatgt ttgtttgata
     7921 tttccggcaa cgacgatcac ttcttcctcc ggttccatag tagatacttc cagatccatc
     7981 aaaagctgta gttgttccag atccgtcgct gtaaaatcag agactcttgc tcctaagcag
     8041 agataaaatt ctggtcgggc aatcaattca gtttgataaa ctgccatatc ttcccaaagt
     8101 tgttcgacag ctgtcatata attggctgca gttgttttca tacatgaagc atgtccacgt
     8161 tggaccttga taaatccttt ttgatcgatg atccgaagac ttccagcatt ttttgaacga
     8221 ttctgttcgc cgcatacata aaagaaaggt gtattttcca ataagcaaac ttcattttta
     8281 tttaatcgca tatcctctgc agcaagctct agtgcttctt ctgttgtgat ctccttggtt
     8341 agatcggttg ctttcacttc cgtagaacca gtagcgatag ccgttacttt tgaagtctgt
     8401 ggatcgattt ctacgtggac ttcgatcgat tctggtgtag cgcctgattc aatcgcttta
     8461 ttcatcgctt catttttcaa cgatcgtata tcttctttac ttggcgaagg gatgatacgt
     8521 tctacaacat ctcggaccat tgcaagtgct acaccaatag aagaaatcac ttcagcgttt
     8581 tcaggaatcg aatatttcac acccatttta ttactaaagt aagtaatcaa tgaggcagcg
     8641 ccgcccccta cacctacaag agaaatctga tctttctcca agtgatactt gtctgccaat
     8701 tctaaaatca ccggttcaat cttagcatag gatttttcca tgatctgctc tgcgatgtct
     8761 tcaaccgtcg tatgacaata gtctgctaat gcctgtatgg cttttcgtgc agatgggaca
     8821 tttccataag aaaaatgttc ttcttgcact aatccaggga cattggctgc acatgtattt
     8881 gtaatagtca cttcttctcc gtcttccatc actaccttga cgtaatcagc agggtctcct
     8941 tctttaggag agaaaaattc aaccttagga cctttgattt tttctgtctc cgtaaatact
     9001 gcataatcta gaccggcaat atgtgcagaa cgtggaccga catcgatgat tcctgactga
     9061 ttagcccgta ccatcgaacc gcctgccaca cctagaaccc gaacatctaa tgaagagata
     9121 tatgttgggt ggccacctac aattgaataa tcgattgctg gacgtccatt tttaatgacc
     9181 ccgatattgg ttgttgttcc acctacttca aaataaacac cattagacgc tctcaagtac
     9241 atcaaagagc ccatgactga tgcagcagga ccggaaagca tggttaaaac aggtcgtttt
     9301 ttcatttcat tgatttccat tactccgccg tctccgcgca taatcatcaa agaaacattg
     9361 acccctgctt ctcgtacgga atcttctgta gaggttgccg tatctagcat ttttggtaaa
     9421 attgatgcat tgattgctgc tgtccgagtt cttcttgtca atccatataa ttttgtgatg
     9481 tcagaagcca tcgttgtagg gatcgatttt accgatgctg cttcataaac aactcgttct
     9541 ggttccccat tatctactcc aaatgccata gaagaaacca agacctccgc tctttctctt
     9601 tctaaagaag aaatcgtttc tgaaacacgg tctacattta agtgtttgac aggtaagaat
     9661 gcattgacaa tctctatttt tttcttattc cctaaatcga tatcgttcaa tctagtttgt
     9721 cgttttgcta aaaaaccttc taatccacct tttgccatcc cgattacgcc gacttttgct
     9781 acgtctcctt caatcagtgc attagtcgct tgtgtggtag aatgtgcaac aaacacaaca
     9841 tcttctggac tgatattatt ttcccgcaaa caattttgaa aagactgaac gacacctgcg
     9901 gctactccac ggacatcatc gtgggttgtt ttcactgaag attttccgat gatttcgtgc
     9961 gtcgcattat cgattgcgac tgctttcgta tgtgtaccgc caacatcaat tcccattcgc
    10021 acaaaacgtt tcattttctt ttcctccatt catctaatcg tttttatcga cgtttcgttt
    10081 actaggcgtt cccgtataat aaaaacgtga tcaggcataa gatcgctccg atgatccaac
    10141 cagttgggat ggatttcttc atatattctc gagaagttac ttttgtataa cctaatcccc
    10201 aagcaaccca agattgtgta atatctaaat gctgaggagc aatcgttgtg attgcaaata
    10261 acgggaacaa aaaggctggc gacatcgttg gattaacagc taacacgaca gctaaaatag
    10321 ctaagcctga accgactaga ttcattggac cgcggaaaaa tcctagtggt gtcaatatag
    10381 caaatacgat tgccaagact aatggtgatt gcggtatcca atctccaaaa atagctgaaa
    10441 aataaggaga agcatacgca gctgaattat tgaacatcgc taaggtcatc aagaaaccaa
    10501 ccattggtgc aacatcgact gcgccatctg taaataattt cgcaaactga cgacagattt
    10561 ctttataacc tccgcgcaat tttccagtaa acagcaatgc ccagatacct gctagaataa
    10621 aacctaagat gattggaaga tctaaaagaa cgacccctaa aactggtaat aatacggcta
    10681 accatgagat cattggtgcg tcgccacctt cagctggtac gtttgctgcc attgcatagc
    10741 gtttcttttt gttcattgat atattagcga ccgttagtac cacaactaag cttacaatca
    10801 tcccaatcat accaatttgg aaatagtcat tataagtata gctttcagcc gctggattga
    10861 aaccagcata aatcgtttga tattgtttga aattgacgat attagcaaag attccagcca
    10921 taatcgagcc cataaatgag aacagtgcaa ccggtactga aatacctaat gacatcaaga
    10981 tcggcaacac aataactgca atagaaatca ctggtccgat tcctgtcatc gacatgaata
    11041 taacagcggt tacaatacat agtaagctca tcgtgattct tggcttgtcc cctccaagtt
    11101 caactacctt tcgaatcagg gtagctgcga ttccgctatc tactagaaca cgcccgaaaa
    11161 aggcaccaaa gaagacattt accaagatcg atttggcgta ctcagctggc cccgtcgtat
    11221 agatatgcgt caacacatca attactcctt gcccttccat cgccgggttt ggtgaaaacg
    11281 tgtttccgac tagcgctaat cccatccata cagtagccat gatggcaaag ccgaccatca
    11341 agttgtagcc ttttacacag taccagacca tgaaaaagaa tgagataact aacaaaatac
    11401 caataataat atcactcatg ttattcctcc gtccattttg tttttctgat aaacaaacta
    11461 atatatcagt taaactcatt ctaacttatg cagttagcgc tttcgttaaa ggattaccat
    11521 aaatgttcgg atttcaatta aaaaacctta attatgctat tttagactta tacgttattg
    11581 tcctgtttca tttgatggag gattgtaaat aaaaaaagag gaaagaacat aacttttcgt
    11641 tatcttcttt cctctttata attgtttagc ttatttagcc aaagcgtctt ttgcttggtc
    11701 agccaatgca gtaaatgctg ctgcatcgtt aacagccaag tcagctaaca ttttacggtt
    11761 gatgtcgatt tcagcaactt tcaaaccgtg cattaatttt gagtagctca agccattcat
    11821 acgagctgct gcgttgatac gagcaatcca caatttacgg aagtcgcgtt tcttttgacg
    11881 acgatcgcga tatgcgtagt tgtatgaatt cattacttgt tcttttgctg ttttaaataa
    11941 agtgtgtttt gaaccgtagt aaccttttgc taattttagc actcttttac gacgtttacg
    12001 agttactgtt ccacctttaa cacgtgccat gtataattcc tcctaataaa aataaatctt
    12061 cagattctgt tatttgtact tcttatttca tttttgctag ttgttggcga atacgtttga
    12121 agtcgccact agaaaccatg cttgctttac gcaattgacg gcgttgtttt tttgttttgc
    12181 cgtggaaacg gtgacttgta aacgcgcgga agcgtttcaa tccgccttta ccagtacgtt
    12241 tgaaacgttt tgctgatccg cggtgtgttt tttgttttgg catgactata tttcctcctc
    12301 aaaatctttc gttatccgac tatcggctgt ccaacagcca gttagtcatt caaattactt
    12361 actgtcgttt ttcggtgcca gcgtcaagaa catgctgcgt ccgtccattt tcgctttttg
    12421 ttccactgtt gcaatatccg cagtttcttc agctaagcga ttcaagactt tttgaccaat
    12481 ttctttatgg gtaatggcac ggcctttgaa acggatagaa gcttttactt tatcgccctt
    12541 ttccaagaac ttacgtgcat tacgaagttt tgtattgaag tcattcaagt cgatcgtagg
    12601 acttaagcga acttctttta cattgatcac tttttgtttt ttacgcgctt cgcggtcttt
    12661 cttttgttgt tcgaaacggt atttaccgta gtccatgatt cgcgcaactg gtggtttcgc
    12721 accaggggca actaacacaa gatccaagtt tgcttgttct gcaatattca atgcttctgc
    12781 ttttgtctta actcctaatt gctcaccatc tgaaccgatc aatcgtaact ctcgtgcacg
    12841 aatgccgtcg ttaaccatca tatcctttgc tatggtcatt cacctccaaa tattatgaga
    12901 gaaagaaaag cttaccgaat aaacggcaat aaaaaaacga gttctcttac agaacccgcg
    12961 cgtacttcac ctaatagaac cagtgaaaac tatctagccc agtgacatta tcaactaagg
    13021 cgagaagcgg gtgcttctgc ttttatttct caaccatcat agtatagcaa aaactaatct
    13081 ttttgtcaat gtttttcata cagtttgtct tccttttctg aaaatgatta gattagcaga
    13141 ctttctagca gtgattcccg cttctttgct atactaaaga aaaacgaggt gaacccacat
    13201 gaaaaaaatc gaacctattc caccttctct tcctcttctt acaaagggag agttttattt
    13261 agaagatgag gagatcttct ctggtcttct gatcaacggg ggtgaccaaa gctatctcga
    13321 tgccaaaaat atggtattaa gagagagtca tatccaaaaa cttacgatgc aaaaaacagt
    13381 tttacgtcat tttgaatgca gtaatgtcat ttttgaaaaa tgtgattttt ccaatctgga
    13441 gtggattggt ggcagtttcc accaagtcgt gtttcatcaa tgtaaattaa caggtacgaa
    13501 ttttgccgaa agttatcttc gagactgtac tttcactgat tgtattgctt cgatggcttc
    13561 ctttagtagt acaaacttga aagctgtctc cttcgatcat tgtcaattag aagacagcga
    13621 attttatgag gtcacttgga aaaacctttt tttgtctgac aatcagttaa ccggttcgaa
    13681 ctggtttcga acttctctga agggattaga ctttacgact aatttcttca cagcaatcgc
    13741 tttatctcaa gattctttgg cgggactaat cgtcaaccaa gagcaagcac ttgtgatcgc
    13801 ccaagccctt ggtttgcaga tcaaagacta gatcaacgaa tccgccgcca cagtgcaata
    13861 agcatccaaa tagataagac caaactgatg atcaaggcga ttccccatcc gaaaacggaa
    13921 tgggtaaagg gtaactggag attcattccg aagaatcctg tcacaatagt tggaacggtc
    13981 aagatcagcg accagaccgt caaaaatttc atcgtgtcat tcaagttgtt attcagcaaa
    14041 ttattataag tgccagagag ctggtctaaa atatgcgaag ctaagttcgt catttcaact
    14101 gcttgtcgtg cttcgatcag tgcatcgtcc aactcttctt tttccttttc tgacaacttg
    14161 cgataaatag ccaatgcctt gatttgttct aaaagtacta cattttgttt tgttcctgta
    14221 actaagtaaa ccaaaccgat ctctaaatct gaaagttgca gcagattctt gttcgttgtt
    14281 ttttctctta atttcagatt aagccgctga cgttcactat tcacttcctc gattaaagga
    14341 aaaaaggaat cagaaatcaa aaacaatgta cgaaacaata aactgtatac actcatgtct
    14401 ggcattcgtt ccaaaagcga tcgaattagt cgaatcacgt actcggtatt cggcgtggta
    14461 aatgtaaaca aaccgtcttc ttttaaaata aaagcgatag gactagtttc ataatgatta
    14521 tcgacttttt gctgatgcgg aacatttgat acaagcagta acgcttcatc aaaagaatcg
    14581 tactctacgc gcgcccgttc atttttatct agagaataag tcagcatctc gtcactaatg
    14641 ccatattctt cttttaagtt tttaacagct agcacatcgt tttgctgtac attgatccaa
    14701 aaattacggt cttgattaaa atagtgtttt tcccgcatac tctatcctcc tgacatttgt
    14761 ttccagtata aagaaaaaag tcgctcaaat aaaggtttac cctttatttg agcaactgta
    14821 aatcaaatgg ctactttggt ttggcgaatc tctgctaatt tttcttcgat cattttcaag
    14881 acttcttctt ttgcttgagg atcttccaca aaattatagc ggtctccatc gatttggatt
    14941 tttggacttt ctttgtattc ttcataccat tgatcgtaac gtctgttcaa ttctttgtaa
    15001 taatcgtata gacttggatc aaaggacaat tgttcatacg aacgaccacg tttttcgata
    15061 cgctccaaca tcgtatcaaa agatacacga atatgaacta ataaatccgg atgtttttta
    15121 tgggcagcat agggaagttc ttccatcatg ttttccagca gttcatcata cacacgaact
    15181 tctgtttctg ttgctcttcc tagatctgca tttagatgga aaagcagtga atcttcataa
    15241 atcgaacggt ccaatacatt attgtcatct ttcattgctt gtttgatgct tgcaaaacgt
    15301 ttattcaaaa aataaatttg cagcaaaaaa gcatactgtt cagggtttgc ataaaataag
    15361 ggcaagactt cattatcatc aatcgattca taaaatgcct gactatcaaa gtgttcagcc
    15421 atcatttctg ttaaacttga ttttccggca ccaattgtac ctgctaaaac gatcacgctc
    15481 atttacctcc agctcacttc atcactaaat attgtgttta actccaacct ttttaaatat
    15541 acccccaata tctagtagtg tcaattgtat ttttttctag tcaaagaaga aaaaaagtca
    15601 aaaaattctt aagaaaaaag tgttttttct ttagaaaacg tattcttagt tttacttctg
    15661 agatggtttt agtaaaataa aggaggaatg ttttttgaga cccttagtgg acaaaaaaat
    15721 tcgtgaagca gcgcaactcg ttttgggcgc tttatttttt gcttaaaaat cacttttacg
    15781 tgagaaggag ttagattatg gcaaactatg gccaaggaga atcaagcgga aagatcatat
    15841 tgatgggcga gcacgcggtt gtttatggag aaccagcgat tgcctttcct ttctatgcaa
    15901 caaaagtcac cgcattcctt gaagagctgg atgcaatgga cgatcaactg gtttcttcct
    15961 actattcagg aaatttagcc gaagctcctc atgcattaaa aaatatcaaa aaattattca
    16021 ttcacttaaa aaaacagcat gacatccaaa aaaacttgca actgaccatt gaaagcacga
    16081 ttcctgctga acgtggaatg ggatcaagcg ctgcagtcgc cacagcagtc actcgtgctt
    16141 tttatgatta cttagcattt cctttgtctc gtgaaatact attagaaaat gtccagcttt
    16201 cggaaaaaat tgcccacggt aatcctagtg gaatcgatgc agccgctact agcagcttgc
    16261 agccgattta ctttacaaaa gggcatcctt tcgactactt ttctttgaac atcgatgctt
    16321 ttttgattgt cgctgataca ggaatcaaag gacaaacaag agaagccgtc aaagatgtcg
    16381 ctcacctctt tgaaacccag cctcatgaaa ctggacaaat gatccaaaaa ttaggatact
    16441 tgacgaagca agcaaaacaa gcaatcattg aaaattcacc agaaacgttg gcacagacaa
    16501 tggatgaatc acaatcactt ctggaaaagc tgacaatcag caatgatttt cttaatctat
    16561 tgatccaaac agcaaaagat accggggcat tgggtgccaa attaactggc ggtggacgcg
    16621 gtggctgtat gattgcatta gcacaaacaa aaacaaaagc ccaagaaatc agtcaagcac
    16681 ttgaagatgc gggtgctgct gaaacttgga tacaaggatt aggagtacat acctatgttt
    16741 aaaggcaaag cacgcgcata taccaacatt gctctaatca aatattgggg taagaaaaat
    16801 gaagaactca tccttccaat gaacaacagc ctttcgttaa cattggatgc attttataca
    16861 gagacagaag tcatcttttc tgatagctat atggtagatg aattttatct agatggcacc
    16921 ttgcaagacg aaaaagcaac aaaaaaagtc agtcagtttc ttgacctttt tcgtaaagaa
    16981 gccggcctct cactaaaagc ttcagtaatc agtcagaatt tcgttcctac cgcagctgga
    17041 ttagcctcct ctgccagcgg gctagctgct ttagcaggag cttgcaatac tgctcttaag
    17101 cttggattag acgatctctc tctttcaaga tttgctcgac gcgggtctgg ttcagcttgc
    17161 cgaagtattt tcggtggttt cgtcgaatgg gaaaaaggcc atgacgactt aagttcttac
    17221 gctaagccag tcccttccga ttctttcgaa gacgatttag caatggtttt cgttttgatc
    17281 aacgaccaga aaaaagaagt gtccagcaga aatgggatgc gtcggacagt cgaaacatcc
    17341 agtttttatc aaggctggtt agattccgtt gaaggggatc tatatcaatt gaaacaagca
    17401 atcaaaacaa aagatttcca acttctcgga gaaacgatgg aaagaaacgg actaaaaatg
    17461 catggaacga ctttagctgc ccagcctcca ttcacttact ggtctcctga cagcttaaaa
    17521 gcgatggatg cggtgcgaca attgagaaag caaggcattc catgctactt tacgatggat
    17581 gctggcccta atgtcaaagt tttagtggaa aacagccatt tatctgaggt acaagaaaca
    17641 tttaccaaat tatttagcaa agaacaagta attactgccc atgctggtcc aggaattgcg
    17701 attattgaat aaagggagat ttttatgatt gaagtatctg caccaggcaa actctatatt
    17761 gccggagaat atgcagttgt tgaaacaggc catccagcag ttatcgctgc agtcgatcaa
    17821 ttcgtgacag tcactgtaga atctgcacga aaagtcggaa gtatccaatc tgcacaatat
    17881 agtgggatgc ctgtacgttg gacaagacgc aacggagaat tagttttaga tattcgggaa
    17941 aatccttttc attatatcct tgctgctatt cgcttgactg aaaagtatgc acaagaaaaa
    18001 aacattcttt tatcctttta tgatctgaaa gtaacaagtg agttagacag ttcaaatggc
    18061 cggaaatatg gattgggttc tagcggtgcc gtgaccgttg caacggtcaa agccttgaat
    18121 gttttttacg cgttgaattt atctcagttg gagattttca agattgcagc actagccaat
    18181 ttagcagttc aagataatgg ttcctgcggc gacatcgctg ctagctgtta tggtggctgg
    18241 atcgctttct caaccttcga ccatccttgg ctccaagaac aagaaactca gcattctatc
    18301 agtgagttac ttgccctgga ttggccaggt ctatccattg agccattgat tgctcctgaa
    18361 gatttacgtt tattgattgg ttggacgggt agccctgcct ctacttctga tttggtcgat
    18421 caagttcacc gttcgagaga agataaaatg gtggcttatc agcttttctt aaaaaacagt
    18481 acagaatgtg tcaacgaaat gatcaaaggg tttaaagaaa ataatgtaac attgatccaa
    18541 cagatgattc gaaaaaaccg acaattactg catgatttat ctgcaatcac tggggtcgtc
    18601 atcgaaacgc ctgctttgaa caaattgtgt aatttagctg aacagtatga aggagccgca
    18661 aaatcttctg gtgcaggtgg gggcgattgc ggaatcgtaa ttgttgacca gaaatctggc
    18721 attcttcctt taatgagtgc atgggaaaaa gcagaaatca ctccactgcc gttacatgtc
    18781 tatagcgatc aaagaaagga aaaccgatga atcgaaaaga cgaacatgtt tcactagcta
    18841 aagcctttca tgataaacaa aaaaacgaat ttgacttcgt acggatcatc cataaccctt
    18901 tgccgcaaat agcagtttct gatgtcgatc taagtacaca ggcagttggg tttacactaa
    18961 gcagcccatt ttatatcaat gcgatgactg gcggaagcga aaaaacaaaa aaaatcaacc
    19021 aagatcttgc aatcgtcgca agagaagcag atttgatgat tgccactggt tcagtcagcg
    19081 ctgccttgaa agacccttct ttagccgata cttatacgat tatgcgacaa gaatatcctc
    19141 acgggaaaat cattgccaac atcggtgcag ggacttccgt cgaaagagcc caagaagcta
    19201 tccgattgtt tcacgcagat gccttacaga ttcatttgaa tgcacctcaa gagttggtta
    19261 tgcctgaggg agaccgtgat tttaccaatt ggaaagtact tattcaagag actcaaacag
    19321 ccattgacgt ccccctcatc gtaaaagaag tcggttttgg catgacaaga gaaacactca
    19381 atgatctagc ttctttagga gttcatacag ttgatatcag cggtcgaagc ggaacaagct
    19441 tcacccagat cgaaaacgcc cgtcgctcca agcgggaact gagttattta gctgactggg
    19501 ggcaatcgac agtctcttca ttgcttgaag caaatgaagc agacacttct atggaaatcc
    19561 tagcctccgg aggtatccgc aatgcctatg acatatttaa agccctttgt ctcggcgcaa
    19621 atgcagtggg tacttcagga accgtactga cgcatctgat gaatcatggc gtagaagaaa
    19681 ctattatact gatgaagcaa tggcaagaag agcttcgtct gctttatact atggtcggcg
    19741 caacgaatac cgcggcccta caccaacaat ctctgatctt ttccggacca gtgaaggatt
    19801 ggtgcgaagc aagagggatc gacttagtga agtatggaag acgcacagaa aagagcgtgg
    19861 gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtacaaacgg cggaagcaaa
    19921 agcaactcct tcggaaataa gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat
    19981 tccttcttat ttcttggagt taaacgcttc tgtcccagcc tcttttttcg aataaatgat
    20041 gtttaatcag ctgtccttcg gtattcgttc aatccgctga tttcacaacc tctccactaa
    20101 acagtgtact aaagctcttt taaggttgtt ttccgatata ggcaagtatg cctccgtcca
    20161 catacaagac atggccgtta acgaaatcag atgcgctaga agccaagaag acagatggtc
    20221 cagccaaatc gattggctcg ccccagcgtg ctgctggcgt acgtccaacg ataaattgat
    20281 cgaatggatg acgttcaccg ttttcttgtg tttcgcgtaa tggagcagtt tgcggtgtag
    20341 caatataacc tggtccaatt ccattacatt ggatattgta ttgaccgtat tcagaagcga
    20401 tgtttttcgt cagcattttt aatccgcctt ttgccgcagc ataagcactg accgtttcgc
    20461 gtcctaattc actcatcatg gagcagatat tgatgatttt accgccgcct ttttcgatca
    20521 tatccggaat aactgctttt gaaacgatga atggggcatt taaatccacg tcgattactt
    20581 ggcggaagtc tgccgcatcc atttcgatca tcggtgtccg tttgatgatc cctgcattgt
    20641 tgactagaat atcaactgaa cctacatctt ctttgatctg ttgaaccatc gcttgaaccg
    20701 cttcttcatc ggtcacatcg cagacataac cttttgcatc gatgcctgct tctttataat
    20761 gcgctatacc ttcatcaact gattcttgat tcaagttatt aaatacgatt gttgctcctg
    20821 ctgtagctaa agagcgtgca atctcaaatc caataccata gacagcaccg gtgactaatg
    20881 ctacttttcc atctaaacga aacatatcca tactaaattc catcattttc tctcccttat
    20941 gcttatttta attgatccat ttgcaccata tccatgtctg tataggtgat attttctccg
    21001 cacattgccc agatgaatga gtaattgctt gtacctacac ctgaatggat cgaccagctt
    21061 ggcgaaataa tggcttgttc attgtctaca actaaatgtt ttgtttcatc tggtttgccc
    21121 atcatgtgga acacacgtgt atcttcattg gcataatcaa agtagacata agcttccata
    21181 cgacgttcat gtgtatgaca aggcatcgta ttccaagcac ttcctggttc taaaatcgta
    21241 taacccattt gtaattgaca gctttcgcat acatttggat ggatgtattg atagatttta
    21301 cgttcattca gtgtcaggcc ttcaccagtt tccattggtt ttacttggtc gatactgatt
    21361 tttacatttg gatatttatg atgagctgga acagaactaa tatagaattt agctgggttt
    21421 tcagcatctt gagaagaaaa tacaacatgt cgtgtttctt ttccgatata gtatccgtct
    21481 tgttttttca tttcttcttt ttgcccatcg atctcgatga atcctggtcc accgatattg
    21541 atgacaatca atagaaggct aggaatcgcc tcttttatta tttttccagc atgagccatc
    21601 tgtccttttt cgacaacagg gtatttattc tttttagcaa cgatgaatgc tactagcatg
    21661 attcctagtg cccacaaagc ccctacgaga taacctccca taaataaagc cgaaatcgaa
    21721 gcaccaccag caacaagtga atacatgata aaagcagtac ttggaggaat gaccatacct
    21781 gtcggagctg acgcgatatt gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact
    21841 tgctgaggga tcaagactcc gccaatcgcg gtagaagcgg caatggctga gctagagatg
    21901 gaaccaaata aagcattccc taacacattc gtatgagcca aagcccccgg tacgcgtccg
    21961 acaaacaaca tcgcaaaatc cactagtttc ttggctatcc ctccattatt catgataata
    22021 cctgaaagaa cgaaaaacgg aatagcgatg agcgaaaaac tgttgatact tcccaccatt
    22081 ttttgagctg aactaaaagc agaaacatca aacggtataa ctaacaataa agtcatcatt
    22141 gatgagaacg caatacttat agcaatcggc atccctaata caagcaacac gacaaataca
    22201 aaaaataaca ctaatccggc ttctattgcc attttattcc tccattttta tattttttct
    22261 tgaattgctg gcttgttgct taatgtatcg attagattga tcagactata gataatgatg
    22321 agtatacctg agacaggtat tgacaaatag acgtacccca tcggaatgct taaagaaggc
    22381 gagatctgtc ccattgtcaa tgtgacagca ttccatccgc catacaccat gatcatcgca
    22441 gcaaatacca gaaataaact ttgaacaacg acttctaaaa tcttactttt tttaccagat
    22501 aatttttcac ttaaaaccgt tacggctaaa tgtgcttttt gtcccacaac atacgcagaa
    22561 gacaacatgg caaaccagac aagtccgaat cggacaagct cttctgtaac cgtattcgga
    22621 tttcctaaaa ccgttctgga aaatacttgc cataaaacga cagcgaccat cccaacaagc
    22681 aaaacagctc ctaggatctc taacacctta tttaaaatca ttttcatcac agctacgctc
    22741 cttgctcttg gatcttttga taaaggtctt tgtaggcagg atcgtttttg aatgtatcat
    22801 gaagcggtgc gactgcctct cgaaacggtg tattatctac atcattgaat tggactccat
    22861 gttcttcttg tgctaaagct ttttcttctt caattgcttt tgcaaaagct tcaatttcaa
    22921 aaaccgttga ttctttcgct gcttcttcaa tggctttttc ctgctcatca gttagctgtt
    22981 ctttgatggc atcattcatg atgatgatat ctggcacacg cgtatgctca tcataggaat
    23041 agtatttagc cacaccacca tgtccagctg tatacaagac aaattcattg ttttctgatc
    23101 cgttgatcaa attggattga agagacgtgt acacttcatc acttcccata ggtacgggcg
    23161 aaccgcctaa aagctctacc attttgatag cggtttcgct ttgcatcaca cggatcttct
    23221 ttcccttcaa atcttccggc gtatggatcg gaccgtctac catataaaag ctgcgctgac
    23281 cagaatcata ataggtcaat ccggtaaatc ctaaattttt ggtcgattgg tagattggct
    23341 ccattatttc ttcattatcc atcaccttaa agaaatgttc ttcatcattg aatagataag
    23401 gaacggaaaa aatcgaataa tctttagaga aactttccaa cgctgaggca cttacttttg
    23461 tgaaatctac tgctcctgac tgtgtcaatt cgatcagttc acgttctcca cccagttgtc
    23521 catctgggaa atactggatc tgtacggaac catctgtttt ttcttttact aaacgcccaa
    23581 attcattcat tgcatcaatt actggctggc tgttgcttgc ataagcaaag cgcaaggtaa
    23641 ctatctcatt tacgtctgca tctgcgacag ttgccacact agaattattt ccgcagccgc
    23701 ttagacctag cagacctagg ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca
    23761 tatgggtttc ctttctttta acaataagag acgagcaatt ctgtcaaaca aagcaccgtt
    23821 aacgactgtc cataaggcat tgctgtgatt ccgatattcc gataaaaatc taaactgtcc
    23881 cccattcctg tcccgattga aacgttttga acttctcctt tttcatcgat ttgttcaagc
    23941 aatccttgga tagcacgata agcgacttct ttatattctt gtggtaaata acgtttatgg
    24001 accgctttca aaataccgta agcaaatcct gcagttgcag aagattctac gtaagaagaa
    24061 ggatcatcca acaacgtatg ccataaaccg ctttcagact gataagtttt taatgcacgc
    24121 acttgtgctt caagtgtttc gatcaataat ttgcgcaatg catcttcttt cgataattct
    24181 aagatttcaa tgatctctgg aatagcaatc gtcaaccagc agtttccacg agcccaaaat
    24241 gcttcggcat agttatgatt tccttcaaac gtccagccgt gataccacaa tcctgttcgt
    24301 ttatccatca agtatttgat atggatcata aactggtaac gagcttcttc taaatattct
    24361 gggcgattca acagtttccc gatttttgcc aaaggaagaa ctgtcatcat caatgtatcg
    24421 tcccacaact gattcttatt ttccggtcca tatgtggcat gctgtaaccc gtcttcattt
    24481 gtccgaggca tgtcattcat gacccattct gcccattgtt ctaaataagg caaatagcgt
    24541 gaatctttcg tgtcttcata caagtaagcc atagtcaata gcggtgccat tgtattgacg
    24601 tttttgggcg gagcgccttc ttccatacga tcctcaaacc attcattgac cacttgataa
    24661 gcgcgaggat tttttgtcat ctggtaattt ttataaatac catatagacc gacaccttgt
    24721 ggccaattcc acactgtcca gctcttgtca tctactttca gtccatcaaa gtccaacaaa
    24781 aattcaccat tatcgtcttt gatcgttgtc aggttttcaa cgagcaggtc aattttttct
    24841 ttcactaatt ctttaggtac aaaaatctct tctttaataa tcatctttac gccacctttt
    24901 taaacgcttt cattttatat ccttaatata gcagtaatac cgtctactat aaatatcgct
    24961 ttttgaacaa aaactgtcta aaattgacct aaatgttaaa taacaaaagt aactttcagt
    25021 tacttggaaa ttaaaaaaag aacttggaga aacaacgttt tccaagttct ttttcatcta
    25081 ttttcttatc ttttttctac gctttctttt gcttatccgt tgtaagcatc aatgatgatc
    25141 tgtttcaatt cagagatcaa tggttctttc ggattagctg tcgtacattg gtcttcgtaa
    25201 gctaattcag ccatacggtc tacggttgta tctagtgttt cttgtgtgac tccttgagct
    25261 tttagactca tgttgatgcc gacagatttt cctaattcat aaacagccgt tgctaaagct
    25321 tcgactaatt cctctgttgt ttcgcctttc agacctaaga attttgcaat atctgcgtaa
    25381 tctgtatctg cacggaaata gtcatattta gggaacatcg catgtttttg tggatctttt
    25441 gcattatagc ggataatatg cggtaaaaga atcgcatttg tccgtccatg agggatcccg
    25501 tattccccgc cgattttatg tgcgactgag tgacaaatac ctaggaatgc gttagcaaac
    25561 gccataccag ccattgtaga agcgttgtgc attttttcac gtgattccat atcaggtgtt
    25621 tttactgatt tttctaaata gtcaaagact aacttgattg cttgtaggct caatccacgt
    25681 gtataatctg aagccatgac tgaaacatag gattcgattg catgtgttaa cacatccatc
    25741 ccggtatctg ctgtgacaga tgctggaact gacatgacga attgaggatc gacgattgcg
    25801 acatctggcg tcaatgcata atctgctaac ggatatttca catgtgtttc actgtcagtg
    25861 atgaccgcaa atggcgttac ttcagaacca gtccctgatg ttgtcgggat acatacgaat
    25921 tgtgtttttt ctggtttgtc gatcttatat gtccgtttac ggatatccaa gaatttttgt
    25981 tttgcaccaa agaaagatgt gtctgggtgt tcatagaaca tccacattcc cttagctgcg
    26041 tccattgcag aaccgcctcc aagagcgatt actgtatctg gcttgaagtc tgccatcagt
    26101 ttcgttcctg cgtatacggt atttgtcgat gggttcggtt caacatctga gaagacttcg
    26161 atcttcacat catttttacg tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat
    26221 tggaccatac cagggtcaca aatgatcatg acacgttcaa cgttttccat tttctctaag
    26281 tacaatagag agttcttttc aaagaagatt tttggcggta atttgaacca ttgcatgtta
    26341 tttctccgtt tcgctacagt tttgacattg attaagttta cagcagagac gttacgagaa
    26401 acagagttct taccataaga accgcatccc aaagtcaatg acggaatcat ttcgttatag
    26461 atatcaccga tgccgccttc tgcagatggt gtattcacta aaatacgaca agctttcatt
    26521 cgaagaccga atttgacatg caactcttcg ttttctgaat ggataactgc tgtatgtcct
    26581 aatcctccaa gattcaacat accttcacac aaatcaaacg catggtccgt gttgttagct
    26641 ttgatcatgg caagaactgg agaaagtttt tctcttgata gcggataatc tgggcctacg
    26701 ccttccaatt cagcaatcag cattttcgtt ccttttggta cactgattcc tgccaatttt
    26761 gcgatttcca tcgcagaatg tccaacgatt gatggattga ccgcatattt tccttcgttc
    26821 atcactgcat cttctaattt ttgcaattca tctggtttta cgatataaac ttggtgtgct
    26881 tggaattctg cttttactgc tgcatagatt tctttatcca cgatcacagc ttgttcagaa
    26941 gcacaaatca ttccattatc aaatgttttc gaaacgatca aatcgttcac tgcacgttta
    27001 atttttgctg ttttttcaat ataagctggt acgttacctg gacctacacc taaagctggt
    27061 tttcctgtag agtaagcaga cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg
    27121 atacctggat ggttcattag catagaagtc gcttcgatcg aaggatgttc gatccattgg
    27181 atacaatttt caggagctcc ggcagcgata gcagcatcac gtactactcg tgctgcttct
    27241 gcagaagatt tttgggcact tggatggaaa gcaaaaacga tcggatttct tgttttcaac
    27301 gcgatcattg atttgaaaat cgttgtggaa gtcggattcg ttgttggagt tactccgcaa
    27361 accacaccga ctggttcagc aatttcaatc aatccttttt ggacatcttc attgatcaca
    27421 ccaactgttt tatcatgttt gatattatgc caaatatatt cggacgcata catatttttg
    27481 attgctttat cttcgtaaac accgcggcct gtttcttcca cagccatctt cgctaatggc
    27541 atatgttgat ctaaagctgc cattgccatt tcatgcacga tatggtccac tttttcttga
    27601 tcaaagcttt ccatttcttt caatgctgca tttgcttttt ctgccaaatc atcaatcatt
    27661 gcttgtacgt caattgtctt cgttttttct ttttccatta ctttagccat cattatcctc
    27721 ctaatgggtc atggttattt gttaaatcat tcacatgtat atcatacaat atttgttttt
    27781 ctatgtaaac acttttttgt gatttttttc acagttattt ttaaatataa aattgcttta
    27841 tttacaggtg tttgtataga aaaatatata aagttattta ttattattta tatgacacac
    27901 ccgattattc aatattgagc aatatatcct atactcttat ctctttcttt tttgaataaa
    27961 aaatgaggta ggacatattt ctgccctacc tcgtctttaa ataagctttg atacttattt
    28021 ttctttcttc tcttctgtct cttccagcgt ttctgttgtt tcagttttta cttcaactgt
    28081 ctcagctggt tcttctgttg tttcaacagt agttgttgca tcatttgtta cgacagtccc
    28141 tggtttgacc gttttgattg ctgcacgatc gtactcaagg aagatccctt cgcagtctaa
    28201 aacaactgtc cgtttgtcat tgtcgatttc tgaaacaact ccatgtaatc cgccgatagt
    28261 tacgactgaa tcacctggtt tcatgctatc caacaagttt tgacgttctt gctgttgttt
    28321 tttttgcgaa cgtgacataa aaaaccacat tgctaatagc gcgataaaca tcacaagcat
    28381 tggtaatgtt cccatttttt tcacctcact aattctgtat ctacaaaaaa cactttagca
    28441 gaaaaatgct gctaacacta gttttttact taatttttaa aagattttcc tttagaagct
    28501 ttttgcgttt tctttattga acccgtattc ttcaaaaaag gcttctctaa attcaagcaa
    28561 attatcatcc atgattgctt gacgtacttg tttcattaga ttcaacaaga agtataaatt
    28621 gtggtaagaa gtcaaacgaa tcccaaatgt ttcatcacat ttgatcaaat ggcggatata
    28681 ggcacgtgta tagtttttac atgtataaca atcacatttc tcatcaattg gacggaaatc
    28741 atgagcatac ttcgcatttt tcactactag acgaccttga gaagtcatac atgtgccatt
    28801 tcgagcaatt cgagtaggca agacacagtc aaacatgtct actccacgga taacaccatc
    28861 gatcaatgaa tcagcagcac cgacacccat caagtaacgt ggtttgtttt cggggatcaa
    28921 cggtgtagtg aattctaata cacggttcat ttctgattta ggttccccga cagataaccc
    28981 gccgattgag taaccaggga aatccatccc gactagatct ttcgcgcttt gacgacgaag
    29041 atcttcaaat cctgctcctt ggatgattcc aaataatcct tgacggtctg ggttagcatg
    29101 agctttcaat cctcgttctg cccatcgaga cgtacgttct actgattttt tcacgtagtc
    29161 gtaactttca tcgaatggtg gacattcgtc aaaactcatc atgatatcag agcccaattt
    29221 attttggatg ttgattgctt tttctggtga taaaaacata cgcgaaccgt tcaagtgatt
    29281 tttgaaatgc acgccttctt cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa
    29341 gccgcctgaa tctgttaaaa tcggttgatc ccagttcata aatttatgca gtccgccagc
    29401 ttcttctact aagtcttcac cagggcgtag ccataaatga tatgtattac ttaaaataac
    29461 gccagcaccc atttctttca actcttctgg ggacattgtt tttactgtag ctaatgttcc
    29521 tactggcatg aacattggtg tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg
    29581 cgcgccagta tgtttttctt tcttgattaa acgatagcga atggcaggtt gcgtcattca
    29641 tttccctact ttctatataa aatatcttga caactcccac catcataatc gtttcaagtg
    29701 ctttttgcaa gttccctcgg cttccttcta ctttatttaa tattcttttt tttatccttt
    29761 ttttatgaat aacaaaaaaa agaaaaacat tatgacagaa atattttttg atacatggta
    29821 tattggtgtt attaaagaaa aggagggaaa aatcatgcaa tcttctgcag aattaaaacg
    29881 ccaagcaaaa gagagtctaa aaggacgttg gggacaagct gttttactaa atctaatccc
    29941 tacattgatt ggtattgcta ctattcttct tattatgatt cctattatct tgtttgccac
    30001 caccatatat gatggatcag cgagcgtctc agaagcgaca acttcaggag gcggtagttc
    30061 aggaggcggt ggcggaatta tttccagcat cattagcgct ttgttcatga gcggtatttc
    30121 ttggacttat cttgatattt tacgtggaca aaaagatcag atcgaaccat tcaaagatgc
    30181 atttcgtgga ttcaaaggtt tgtttatcgg cggagtgatc agcttagctg tgctgatctc
    30241 gatctttaca acattatggg gtttgctatt agttattcct ggaatcatta aaagctattc
    30301 ttattctcaa agctatttta tttactacga tatcgtccaa gaaacaggac aaacacctaa
    30361 agtactggat acgatcactg caagtcgaaa attgatggat ggttataaag gacaactttt
    30421 ctggttagat ctttcattta tcggctggca tatcttagca ttagcaactg ctggtattgg
    30481 ttatctatgg ctcagcccat acatttctgc aacaaaagca gcattttatg aacaattgcc
    30541 aaaagatatt taaaagagag tgtgacttgt gaccgagggg gtggcaggag aacaccgttt
    30601 atcgagaggc agtaaaagaa acattcagcc cattagcaaa aaggaggtgt gacaacattc
    30661 atgtcacacc tcctttttgt atagttccag taaaaagaaa atcaacttag aaacagttaa
    30721 tcattcctct tcttctgctt ccggtaatcg atggaacttg atcagttcat tcaccgcgat
    30781 tggtacgata gataaaatcc ctaccaagaa ccagtgattc aaactgatgt gtactaggcc
    30841 gaatagttct tgcgtaaatg gcagcaaagc aacagccgtt gtaatagcta atgccagtaa
    30901 cgccatttct accaaagctt tattggtcgc taaattgact ttgaagatcg attggctgct
    30961 gcgtacattg aacacatgca gaatcgatga ataagctaag atcaagaatg caacagtttg
    31021 accgacatgc tgacttgcgt ctacccatgg gctgatagta tcgacataac tacctaaata
    31081 ataaccaaac aaggttacga tggtgaagac ccctgcattt atggcaattt tttgcaataa
    31141 acctcgagca aagatccctt catttttcgg aatgggtggc tgatccatga tgccgtgttc
    31201 cgctggctct cttcctaaag caaatcctgg aagtccatca gcaactacat tgacaaacag
    31261 tagttgcaca gctgtaaatg gtgctcccca tcctaacagc atcgcaatca aaacaataaa
    31321 gatctccgaa atattacagc ttagcaaaaa gttgatagct ttacgaatat tctgataaac
    31381 cgaacgtcct tgactgacag cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat
    31441 atctgcggca ctttgcgcca catctgttcc tgtgatcccc atggcacacc caacgttact
    31501 tgctttcaac gctggtgcat cattcacacc atccccagtc atcgctacga ctgcacccgt
    31561 acgttgccaa gatttgacga tccggatctt gtcttctggc gttacccgag catagacaga
    31621 gagagctttg acacgcgcat ctaattcttc gtctgataat tgatgcagtt ctgatcctga
    31681 aagtgcttct gacggatcat tcaagatacc taattctttc gctatggcac tggcagtcac
    31741 cacatgatct ccagtgatca tgactgtttt gatgccagcc tttttcgctc gcttgattgc
    31801 tcctttactt tctggacgtg gtggatcgat cattccgatc agaccaatca aacgtaagtc
    31861 tttttccagt gcttcagaag aaatatcttg cggttcttcg tcataagtag cataaccaac
    31921 agcaatcact ctcagtgccc gtttaccaaa gcgatcatta acgatcgccg cttgttctac
    31981 atcgcctgaa ctaaagcgcg gagcaagaac atcgaatgct cctttggtga ttgagatgta
    32041 tttttcgccc atttggtgaa ctgttgtcat catttttcgt tcagaatcaa aaggaagctc
    32101 gtttactcgt ggatattttt cttctaattc tgcctttgta tgataattct cttcaacagc
    32161 ccttacgatg gctacttctg ttggattacc tttgataaca aggtcgtcat cttcttgctc
    32221 gaccgtcaca tctgtacata gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat
    32281 tgaatcttcg atattcgtta cttcatcttc tttgctccat acccggcgga cgcgcatttc
    32341 attttgtgtc agcgttcctg ttttgtctga gcagataaca ttagcggttc ctagcgtttc
    32401 aactgctggc aactgtcgga tgatcgcatg tttcctcgcc atcttctgca caccgtatgc
    32461 aagtgtcaac gtaacaataa cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat
    32521 cgaagtcatg aacatctcaa ggagtggttc tccttgcagt tcaccaatca caaatactaa
    32581 agccgcagcc gctaaagcga tcatactgat tcgtttgcct agctgattca atcggacctg
    32641 taaaggtgtt ttttgcatag tattttcgtt caaaagatcc gcgatcttac ccatttctgt
    32701 agccatgcca atggcagtga cgatcgcttt cccacgacct gcagcaaccg tacatccttt
    32761 aaataccata ttaagttggt cacctaatga ctcatcttct tcagcaataa atgctgcatc
    32821 tttttcaact gcttcacttt ctccagtcaa cgccgattct tctacacgta attgtgatgc
    32881 ttcgatcaaa cgggcatcgg cagaaatagc atcaccggac tctaaaacaa gaatatcacc
    32941 cactaccagt tgctctgact caacagtggt cactttgccg tctcgtatga cagttgccgt
    33001 ttgtttattc atatcttgca aggattccag tgctttttct gcgtttcctt cttggacaat
    33061 cgccaatacc gagttgatca caacgatagc gatgatcagc agtccttcaa ataaatcccc
    33121 atgttctgta gcaaatgcgg tataaaagga aatcaccgct gctacaagaa ggatgatcgt
    33181 tgtaaaatcg gacaagcttc gaaagaattt tttgatcatc gattctttcg gcgcctcatc
    33241 gaatttattc aatccctgct tttttcgccg ttcatccact tcttgtgaag acaacccagt
    33301 atctggatct gcttctgtta ccttctttac ttcttctatt gtctgcttgt attcttccat
    33361 cctattcctc ctttaaaatc ttcgtacaaa aaaaggtact tattttacta taacatcctt
    33421 tttttattta cttcaaataa agggcttatc gattatttgt taagaaagcg taaaatttaa
    33481 agtgcttata tttgcaaacg ctaccaaagg atgctattat atagatgtaa ggaaggagga
    33541 taccatcaaa tagacagaag aatttctcga tagaacagtg taagcaaaca aagttgttag
    33601 aagtacaggt ttcacccaga catgcgtgta gagtacaaac ctataaatag tgaatgcgta
    33661 tggatgtttg gatcacgaag tatccgaaac aagaagtaat gcgatacgta acaattgaat
    33721 aacgtaaacg tctgaaaatt gaataggaac aagagatgtt tcaaatgttg aagatgtaac
    33781 gaaaacaaaa tttttgttta acatgaagaa attcaacacc gtgctttgca tgtatgatgg
    33841 aaagttacaa tcatttctgt ttatttgaat ccttccgagg tacaagaaag ccgagcttat
    33901 cgagagctcg gctttttctt tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac
    33961 tagcggcttt tcataacctt atttcacgaa catcgcatct ccaaaactaa agaaacgata
    34021 gcgctcttca atcgcatgct ggtaagcaga taaagtcaat tctcttcctg caaaggcact
    34081 aactaacatg actaaagtcg attttggcaa atggaaatta gttgagaaag cttctaccac
    34141 tttgaattca tagccaggcg taatgaaaat atcagtccat ccgctgtctg ctttgatttc
    34201 tccattaaat ttcgttccaa tcgtttctaa tgttcgaatt gaagttgttc cgacagccac
    34261 gattttcccg ccatttttac gtacttggtt caatcgctca gccgcttctt ccgtcaaacg
    34321 gtagaattca ctatgcatat ggtgttcttc aattttatct acgctgacag gtcggaatgt
    34381 acctaaccct acatgtagtg tcagatagac taattcaaca ccctttgctt cgatctcatc
    34441 aagtaattct ttcgtaaaat gcaatccagc tgttggcgct gcagcagaac cattttcttt
    34501 cgcatagaca gtttgatagc gatctgggtc ttccagacgt tctttgatat atggaggcaa
    34561 tggcatttct cccaatgatt caagaatctc tagaaaaatt ccttcatact caaagtcaat
    34621 gatccgtccg ccgtgttcca attcttcttt cactactgct tgcaatctgc catcaccaaa
    34681 agagattctt gtcccaactt tggcacgttt cgcaggtttg atcaatgtct cccacgtatc
    34741 tccttgtgta ttcgttagaa gaaggacttc taaatgcgca ccagtatctg gtttttctcc
    34801 atataaacga gcaggcagaa ctcgtgtatt attcataact agcgcatcac caggctgtaa
    34861 ctcttcaatg atttcatgga aatgtttatc ttccatctct ccagttttcc gatccaaaac
    34921 aagtaaacga gaatgatcgc ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa
    34981 gtcaaaatca aaatcttctg ttgttaacat atcgtcactc actttctatc atgtgcttca
    35041 tcattctatc atgtttttcg ttctgttggc aaaatcagca ctttttcact tctatttttt
    35101 gttttcctaa ataaaaaaac taccctattt gtattttttc attgttttag gcatactagt
    35161 aatagatagt tcttcattga aaatagaaaa agaggtggca ccatgattga atttcaaaat
    35221 gtgtcaaaaa tttataaagg tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa
    35281 aagggtgagt tcatttgttt tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg
    35341 atgatcaacc gaatgactga tccaacacaa gggaaaattt tgattgatgg aaaatccatc
    35401 caagaaattg atccagtgga attgcgacgt cagatcggct atgttatcca aagtattgga
    35461 ttgatgcctc acatgacgat ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc
    35521 agtcaagaag aacggaataa aacagctgaa aaaatgattg atcttgtgga attgccccgt
    35581 gagatgttag atcgctaccc acatgaatta tctggtggtc agcagcagcg tattggagtc
    35641 gttcgtgccc ttgcagcgaa tcaagatatc atcttgatgg acgagccatt cggtgcgctt
    35701 gatccaatca ctcgtgattc cttgcaagac cttgtcaaag atttacaaga gcgtttggga
    35761 aaaacgatcg tatttgtaac ccatgacatg gatgaagcga ttaaactagc aagccgtatc
    35821 gccatcatga gtgaaggccg tgtgatccaa tttgatacac cagaaaatat tttacgtcat
    35881 cctgcaaacg atttcgtaga agagttgatt ggagaagatc gcctactaca agccaagcca
    35941 aatgcgacaa gagtcggcga agttatgcta aatacagcaa tcacgattac accggaaaaa
    36001 tcattacaag aagcaatcaa actgatgagg gaaaaacgtg tagatacgtt gttagttacc
    36061 gataattcga atgtattaaa aggatttatc gatgtagaaa cgttgaataa aaaacgagga
    36121 aaagtatcca gtgtcggcga cattttgaat aaacatgttt tttacgtaaa agaatccgcc
    36181 tatcttcgtg ataccttgca acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt
    36241 gatgaacaga acaaagtcgt aggtatcctg actcgtgcat cattagtgga tatcgtctac
    36301 gatgtgattt ggggagaaga tcccgcaact actgatgttg cagagtcagc tactcctgaa
    36361 gaatctaagg aggtctgacc tatgcagcag ttttttcatc agcacggttc agaaattctc
    36421 tcaaaaggtt ttgaacatct ttatatttca ggacttgctt tgatcattgg tatcatcatt
    36481 gctgtaccgc ttggtgtctt actgacacgt tttccaaaag ctgccacgat tgtgattggc
    36541 ttaaccagtg ccttgcagac aatcccttct cttgctttat tggcattgat gatcccgcta
    36601 tttggcgtag gtaaattacc tgcgattatc gcactcttta tttattcatt attgccgatt
    36661 ttacgtaata cgtacattgg gatgaaaaat gtcgattgga attatcggga tgtggcaaaa
    36721 ggcatgggga tgactgagtt ccaatccatt ttttccgttg aattgcccat tgctatgcca
    36781 acgatcatgg ctggtattcg tttagctgcc gtttacgtga ttgcatgggc tacacttgcc
    36841 tcttatatcg gcgctggtgg tttaggcgat ttgattttta gtggactgaa taattaccaa
    36901 ccagatctca ttttggcagg tacgatccct gtaacaattt tagcactatt ggctgattgg
    36961 cttctaggtc tcttggaaca ccggctgaca ccgatcgcac ttagagagga gaatgaagaa
    37021 tgaaacgtat actaaaaatg accatggttc ttttctctct tcttatactt gcgagttgtt
    37081 ccttccctgg tcttgccagc aatacagatg aagacaccat ttcgatcaca ggtggaatca
    37141 cttcagaagc acagatctta gcaagcctag ttgccggaat ggttgaacat tatacagata
    37201 aaaataccgc aatcatcaac aatctggcta ccacaactat caaccatcaa gcgatgatga
    37261 acggagatgc ttcaatttca gcggctcgct acacaggaac agatttaacg actacattga
    37321 accttcctcc tgaaaaagat ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga
    37381 aaagatacga tcaaacgtgg ttcccgtcct atggttttga aaatacgtat gtctttcttg
    37441 ttcgaaaaga caccgcacaa aaataccatc tatctaaagt cagcgatctg aaaaatgtag
    37501 cggatgaatt agtagctggt gtagatactt cttggatcaa tcgtaaagga gacggttatg
    37561 acggcttcca agaaacttat ggcttctcct tcaattcgat attacctatg cagattggac
    37621 ttgtttatga cgcagtcgaa gcaggaaaaa tggatattgt tcttggctac tctacggatg
    37681 gacggatcgc cagttatgat ctagtcatgt tggaagatga tcgtcaattc ttcccaccat
    37741 atgatgctgc tccagttgta gacaacaaga tattagctga tacgcctcat ttaaaagaag
    37801 ccttaaataa gttaggtcat accatcagta cggaaaaaat gcaggaattg aactacgaag
    37861 cagataataa tctagtggaa ccatcagtag tagcacaacg tttcttacaa gaaaaccact
    37921 attttgaagg gaagtgaagc aattggataa aatgaatgtc tttcaacaat ttttctatta
    37981 tttccaggaa aatggctcct atatctttgc acaatttatc cgtcacttcc tgatttctat
    38041 ctatggtgtc ctctttgcag cagttgtcgg gattccggtt gggatcatga tttctaggag
    38101 acgtaaatta gcaaactggg tgattcgttt ggcgaatatc atccagacga tcccttctct
    38161 agccatgatc tccattttaa tcattggatt aggcctggga gtcaatgtcg tgattgttac
    38221 tgtatttttg tattccctct taccgatcat caagaatacg tataccggga tgatccaagt
    38281 agacaaaaac atccttgatg tcggcaaagg catggggatg actgctagac aacgactatt
    38341 catggtagag ttaccactct ctgtttctgt tatcatggca ggcataagaa acgcgttagt
    38401 tgttgccatc gggataaccg cgatcggtgc attcgtcggt gctggtggtc ttggagatat
    38461 cattatccga ggaaccaatg caacagatgg cacatcgatt attttagcag gtgcattgcc
    38521 aactgcatta atggcgatca tcacggactg gcttttaggt attttggaaa gacggctaga
    38581 tccagccagc cgtacatcac gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt
    38641 tgttggtcgc aaaggctttt ttagttctgt cattttttaa ggcaataatc cactctgatt
    38701 cttccctcct tatgggcaaa gagttgcttt ttttaactat ttgtccattc cgtatactcc
    38761 aacttcatac tgccaacggg ttcatctctg catccaccga ctaagttttt ttcttatccg
    38821 ctaaggcatt ttgccaatcc attttcccat cactcgtcat ggaattcata ccacatgatc
    38881 tctaaatact cgtctgcttg ttctttgaat tgggcatcaa ttcttacatc ctttggcgtg
    38941 gctttcattt aagccttttc catcagtcct cgattgttcg acgccttcag ctgcatcatc
    39001 aaaactggtg actgccgtat cggtaacttc gatcatcttt tctacaatcg ggaacttgcc
    39061 atcctcatcc ttggctaaaa acatatctag ccattccagc ttctcgccat gggaggtttg
    39121 cggttcctaa aaccggtaca cgattcttat ggactgcatc cgtcgcaact agacttggcg
    39181 atacgatgat ccttcaccag aagagcaccc ccgtaaacca attgatcgat atattgccaa
    39241 taagaaaaga cattggcgta aaagatatcg atccctcgtg atgcattgcc gttgatactg
    39301 ctattcatgg ccgaaagcgc cacgactttg acttcttccg tttgtgtgac attcatgttc
    39361 ggcaatgtcg ctttggatac ccgctctgct aaaggaacga cactggtatt gtatttcgta
    39421 tctgtatctt ttccaaaaga ccatgccaac gaatcctttg ggaaccaata agacgactcc
    39481 ggttgattgt ccattgcttt tttcaacata cttttggctg tttctcgata aacgacattt
    39541 ccaattgtct tcgcctcact ttggcgataa tcgatggtcg ttgccactgc caacactaac
    39601 aacgatgctc caattttact gattttcctt gtcacacttc ttcctccttg ttttacataa
    39661 acccgtcaac attccattcc taaactcaaa caatgaccct tcaaaggtgg agatatagga
    39721 acaaattaaa gaaatcatgc aataaatcca agaaggttgg ttttagaaca aaaaaagagt
    39781 ctcctttttg gggagactct ttgttaacta tctaaaaagg ttggctcttc tcacttcact
    39841 cataatgtgt ccaggcagaa taaatttcca caactttatt ttcctcatca actttgtaaa
    39901 ccactcgatg ctgtctattc agcctgcgtg agtatctgcc ttcatgtgta ggtctcagct
    39961 tttcaaaaga ctgcgtgggc atatatggat cttccttcaa tgtctgaatc acttcttcaa
    40021 attgtttctt taaattcgat tgcttgattt ttcttaaatc cactttcgca gagtttttga
    40081 ttgcgactgt atagttactc atcaaagagc ttcccaatca atatcatcga tattcgtaaa
    40141 gccactgtcg tctttttcgc ggtctcgtac tttatctaaa gtacctgttt gctcaagaaa
    40201 aagggtttct tgaatcgctc tccagtcttc tagcccaatc aatacagcat tgtttccact
    40261 acgatcacta ataatctcga tttcagtatg attttcattt acctctttca ataactgata
    40321 aaaattttta cgagcagtgc taggattcat cgttgtatta gccattgtct ctcctccttt
    40381 ttctgatata attatagcgt acttgattaa gtacgtcaaa aaatacaata aagcacttaa
    40441 attcagcaaa cttttcctct tttttacata gagaaaaaga ccccttttta gggaggatct
    40501 ttgtccacgc tcacttctta ctcctttacg actagcggta gccaaacgta aagtaattgg
    40561 taacaacgta tcatctttag caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca
    40621 ccaatacacg aaggttcctc gttgcagtgt gacttgattg atatcttgaa taacctgagg
    40681 atacccacga aagatttgta acggttattc aaaactgatt ttggccgtca agtcttctac
    40741 tgcctcaaaa atgattgcct ctttcgtctc ctgccattca tgttcactat cgatttgata
    40801 cttgcttaga ccgtcgggtt tgtgaatcac caattaaaaa tggcactgag ttatggtaac
    40861 aaaaaatggt cacttgacga ggctgagatt tctacttatg aagacacatg gcacttaaga
    40921 gtcgaggtaa aggaagaagc tgaaaatata ggaggctgta cattatttgg caaaggattc
    40981 ggaaattatg atcaagacat caaaatcaaa atgttttaca gctaaatagg aagaataata
    41041 acatttcata cgctgttccc atctttaatg gcttatagag ggcaccttta gctgggaata
    41101 agacaaaaca gaccctctag cttcaattaa gagctagagg gtctgttttt gattttaaaa
    41161 cttggtttat ttcttcaaat ggtagctata ggtgctgaca acgatatttt cacgccaaga
    41221 caatcgcaat cttaaccgca aacttgtttg gttatggaga atattctgcc agcgtctgtt
    41281 tggtacaaat tgaggaatca acacagtagt tgtatagttt tgtttcgctg catttttact
    41341 tacgatatct acataacgga taattggatt ttcaatcgag cgataagaag agtggacgat
    41401 tgaaaaacgg acatctggga aatgcttttt aaattctgct tcgatttctt tttctttttc
    41461 gacatcttcg tctagcgaaa catgcatcgc aacgacataa tcaccgatcg aacgtgcgta
    41521 attcaacgca ccgatattta cccgtgtcac attcccgact aatacaagaa cggtattccc
    41581 gtcataatca tgtagttttg catcattttc taaacgaagc tgttctgcta cttttttata
    41641 atgatcatgg atcttataaa aaatgaacat aacgattggc ataatgatga agaaaggcca
    41701 aatgtcccct aaccgataga cgaacaaaat cgcaatgatt gcatatgaga tgaacgcacc
    41761 tgtaatattg gcaatcgact tgcttaacca tctttttcct tcttttttcc atttgacgac
    41821 catccctgtt tgagataaag caaatggaat gaacacgccg atagagtata acggaatcaa
    41881 gcgttctgtt gagccatgga aaataaacag caatacgatc gaacctagcg ctagtgtgat
    41941 aatcccattg gaatagccta gtcgatcccc acgatcttga tacatgtgag gcatgaattt
    42001 atcttttgcc agattgtatg caagaactgg aaaagcagaa aaaccggtgt tagcagcaac
    42061 tgctaaaatc agcgctgtag caaattgaag gacgtaatac aaaataccat gtccaaagac
    42121 agctttacca atttgagaca aaacagtgac ttctttttca ggaacgatcc cataccaata
    42181 gttgataaat gtgatcccca cgaagaagaa acctaagatc aatgccatca tcgtcaatgt
    42241 agcagcggca ttttttgctc ttggcttctt aaagaatggt acagcattac taatcgcctc
    42301 caccccagtt aaagaggaag aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac
    42361 acccggcaca gctgctcctg gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat
    42421 attatataaa ccaacaacga tcagtaacga gatcacaagg ataaatgtgt acaccggaaa
    42481 taataggaac gatgcagatt ccctcagccc acgaagattc agcatcatca gtagcaaaac
    42541 gattgtcaca gaaatagcga cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc
    42601 ttcagcacct gctgagacag atactgctac tgtcaacatg tagtcgatca ataaagagcc
    42661 accggaaatc aagccagcat tctttccaag attctcactg ctgacgacat atgctccccc
    42721 gccatgagga taagcatgga tgatttgacg atatgatagc gttaatgaaa tcaataaaat
    42781 aataacaaaa gcggcaatgg gtagcgaata ccagatggct gcagcagaca aagcgactaa
    42841 aacgacaacg atttgttccg taccatatgc aatagatgat aacgcatcag acgacaataa
    42901 tgccaaagca gcaaatcggg ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt
    42961 acctactaac aaccttttta agtaatccaa tttctaaact cctttacaca caattttact
    43021 tcaagtttta tagtcgaaaa tttatagtaa acaagatgtt atagtacgct cactgacctt
    43081 aaaagtcaat aaactttaag aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt
    43141 tcaaacgctt taatacagca aaaatctaac atttttttat gggctttttc tttgaattct
    43201 ataataaaag gatagaactt gacttagaaa atcaagttct atcctttttg atatcttaac
    43261 ttttgaaaga gagatgtaag gttgctttct acccatcatt cttactactc gaagcatagc
    43321 gcagttgtca caacctcttg ataaacggtg tttaaaagct tgcatctgcg gtaataagcc
    43381 caaccaaatt aaaaacataa gaagctgttt ttatttggtc gttcttatta cttgatgcag
    43441 aacagccttt tcacaacctc ttggtacacg gtgttcaatc agctgaccct cggcattagc
    43501 tcaaaaaacg gcaaaatatg aggaactatt tttccatttt tttctctaat actcaggtca
    43561 aaacgctgat ttcacaacct ctttttttaa tgaaaaatat ggctctcatg cgccgcttct
    43621 ttgaatttcg tcattttttc ttcgatgaac ggatagattg ccgttcctac gattccaaag
    43681 acaacaaaca gaccgatcat gatccagatt gctttcgtag catttggcca ataaattcct
    43741 cctgcagatt cccgtaacaa gtttaccgca tgagtaaatg gcaagaacgg attgatcatt
    43801 tggaagaatt tccctgagac ttggatcgga tagttcccgc ctccgccaga gatcgacagt
    43861 actaagatga taatagcgat ccctttaccg acattatcga ataatgcagc caagacatag
    43921 acgatcatca tgaacgctaa ggcgatcaag acagcaaatg acacgctgta gaccggattt
    43981 tggacatcta ctcctagtaa gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc
    44041 gccattgtca aaaatgtcag catacgagca acgaattgtt ctcgtttcgt aaaattgttt
    44101 cggtctttct ttcctaaata gtattctgtc gtagcaacac ttgaaagaag aacagcgccg
    44161 acccacagac atagtgcagt ataaaacggg gtgctagctg aaccgttatt tgcgattgga
    44221 taaagcgcat tggtttgtag ctcaacaggt tgtgtaaaga agtcactttc ttttgttgca
    44281 tctagtttca ataatttgat gacttcgcct agatcgactt cttgttcgcc ttttcggatc
    44341 gcgtctgctg ctttttggat accagctttg atatttggcc agtcattgtt gatcaagtcc
    44401 gcagcagcgt tcacagctgt ttcgacttct ggcatttttt catttactgt tgtcaatgta
    44461 ccggtaattt cattttttag tgctggccaa tcattttgga taaaatcagc ggccaatcct
    44521 aatttttgct gaaggatcgg taattcattg ttgtataggt ctgccccttc attgatgcca
    44581 ttgacgatgg tatccatatt tccattcagc attgtattgg catcatggat ttcttgtcca
    44641 attgcaggca tttctttttg atatttttct aaaattgcca ctgcattcgt tacagttgct
    44701 ttcgttgaac tgagaagcga atcaaagtcg atctggtccg ctttgcttag cgccccttgt
    44761 gcagtcccaa tcgtatcgat gacttggtct aatagagaag tgacagtgga actgatttga
    44821 cctgtattga taccagcaat gatgccattg atattattaa caacggcatc aatttgatct
    44881 agcaattcat caatagcctt cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg
    44941 cggttttgca gattgcttag caagggtttt aattgattta gctgatcaat aattggctgc
    45001 aatttcgtat ttccagcact tgtttgcaat ttagtcagga actcgaccag tttatcgatt
    45061 gcattttgtt gttgagatat tccgtcaata aagccgttca atacattgct gatgtttttc
    45121 ttttcatcat cagtcagctt accatcttca atagctgttc ttacagtatc tattaacgaa
    45181 cctgtcgttg ttgccacttg tccaattgac ttcaatgtaa catctacact gtttgtgatg
    45241 cttggtatcg catctttcag ctgagaggca cctgattttg tttggtttgc tagatcactt
    45301 gcctgatttt ctaaattttt cacatctggc aagatatcct gtacttgttg gatgatcgtc
    45361 aagccttgtt ttgcttcatc gattccttgg ttcattgtct cttggatcga agcaaaatcg
    45421 ccgtcgatct ctgctagttg tttaccggca ttttggatct ctgggatttt ttcttgcaaa
    45481 tcaaggatga tttttgcttg atctttcaac tcaggcattt tgtcattcaa tgcaacgact
    45541 ttttcaccca tctcatctac tttagggatg taatccacga attcatttgc tttattcagt
    45601 ttttccttga tttcaggtag ttgactttgt aattcgagga cttgttttgt gtagccgtcg
    45661 atcgtgtcca aattttcatc tgttgataaa atcatatctt tgactttatt gatacttact
    45721 aggttcgtat cgatgtcgta acctatctca ttgaatactt tcaataaagt cgaactggct
    45781 gttttgataa attcatttgt gatttgcgat tggatcgaac ttgctccttt atcggtgatt
    45841 ttcggtgcaa tcgcattgat tttttcattt accgtatatt cgatttttgg tttcttgata
    45901 tcaccactag taaaactcag cagatcttct gagaaatcct ttggcaagta aatacctgcg
    45961 taatactttc cggaacggac tccgtcttct aattgttctt ttgaatcaac gaattgccat
    46021 cctaattgtt tatttttatg aagcgattcg atgacttgct ccccaatggc cacttcttta
    46081 ccttgaaact ctgctggctt gtctgcactg taaacagcaa ttggcagctc tccagtattt
    46141 ccatatggat cccataatgc tttgatgtta aaccacgcat atagcgaagg gatgatcatg
    46201 atcgcgatga tcagaagtgt tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa
    46261 tataatcgaa gcgtattttt gattgactgc atagttctcc cccttcttct tttttcagct
    46321 gaattacttt accatgtatt tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat
    46381 ttttggacat atcacggcaa ttgtctcatt gtaatctctt acatattgag aaataattaa
    46441 ggattacagc ctctacttta tcatgaaata aaagggaaag ataattttat gccaaagttt
    46501 ttatctatta tacgtatacg caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa
    46561 atgtttcagc ttttttcgtt ttttattcct gtatttctta tttttcgaat gacaggatga
    46621 ttttcccagc aggaggatgt ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg
    46681 aagcagacag attcattgaa ataaaaacat gaaaggctcc tttttggtag gctttgatta
    46741 tgccagcaaa ggcttctgca tctagatatt cctttcttgg aacaaacgat tcataaaagc
    46801 cttccttttt ttgccgcaaa tctagatctg ggaggtcatt taatgcaaca tacctgccgc
    46861 ctggtttcat gatctggatg ccggtttctc ctttgatact tcctttcgtc gcatcgatta
    46921 caaggtcagc ctgatcagca aattgcagac ctggatttgt tttatcataa gcagcaaatg
    46981 ctgaagctcc taattttttt accttttcct catttttact ggatgcactg gttaagattc
    47041 ggattccctt ttcatggaga agctggatca gacttgaccc gaccgcacct gatgctccta
    47101 gtatcattac agtatctgtc ggctggattt ccgtcaaatg gttgattaga tgataagccg
    47161 taattcctgg cgtgaccatt ccggctgctt cttcccaact cattttatct ggaatttttg
    47221 ctacttttgc tgatggcaac acgacttctt ctccgtaagt cccgccgact gcatggacag
    47281 ctactcgatc acctacatga acagttgtga catctgaacc aatctctgtc acaataccag
    47341 cgccatcatt acctggtaca taagggaatt tcaatgttcg tatctctttc atttctccta
    47401 aacgcaaggc aacatcataa ggattcacac tgaatgcttt tatttctaca cgaacatgtc
    47461 cagaaccgac ttctctggga tgagcatcga tttcttcaaa aacatcttgt gcttctccgt
    47521 aaccgttaat tgcaaatctt cgcatggtca ccatctccct ttttctataa gtttacgttt
    47581 atgcctttat tttgacaaaa aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat
    47641 agttgtcttt tttcgcgaaa cgtgttaaat tatttatcta aaagcgtaaa gattacgatt
    47701 tacgctaata aaaaataatt ggaggaattt gttgatggct aaaaaatcaa agattgccaa
    47761 agcaaaaaaa cagcaagaaa cgattgaacg ctatgctgct ttacgccagg aattaaaagc
    47821 gcaaaaagat tacgaaggct tgcgcaaact tcctttagat gctcgtccag aacggttgaa
    47881 aaatcgtgat ttaatcgatg gacgccctcg cggatacatg cgtaaattcg ggatgtcccg
    47941 tataaaattc cgtgaattag cacataaagg tttgattcca ggagtaaaaa aagccagctg
    48001 gtaatccaag tctggctgta atgtaaagga ggagggcgca tggaccactc cttggatcat
    48061 caaaaaagat acgttcgctt cttctagcag aaatgctggt actagaagca gacgtatctt
    48121 tttttacgca cagctggtcc ttttttatat gtaaaggctg catggttact tcgctatatt
    48181 tatttagtaa caggagtcag tgcttcgata cccacactaa ttttcaatgc tcggtcaact
    48241 tctgccataa tgggtcgatc caaatggcag accttttctt tcaatcttga tttatcgatc
    48301 gttctgatct gctccaacag gataacggag tctctttcta ttcctgttcc agtagctttg
    48361 atcccaatgt gagtcggaag cttaggttta gccatttttg ccgttatggc cgcaacaatg
    48421 atcgtcgggc taaaatggtt acctagatta ttttgaataa ccaaaactgg gcgtgttccg
    48481 ccttgctccg atcccacgac tggagaaaga tcagcaaaat aaatatctcc tctttttacc
    48541 ataggatcct ccttattctt gatattctct tggtatccgc tgtcctaatc cgcaagccac
    48601 ttcatagtgg atcgtaccta attgatctgc tatatcctgc atagtgattt ctttgtctgc
    48661 atttttgccg ataagcgtca cttttgttcc gacagggaat tcttgaggca atcggatcat
    48721 cagttgatcc atacatacac gcccaatcgt ttcacagtag ttcccttcta ccaatagaga
    48781 aaaaccctgc atctttcgtg gccagccatc tgcataaccg atcggtatcg ttccgatcca
    48841 ctctgcttca ggagtgatat atgtttctcc atagccgatt ccttcacctg ctgggagttt
    48901 tttgacttga atcaattcag aaaccagctc aagtgctggc tgcaatggat aaacttccgg
    48961 tagtgcgtgc ccagagggat tcagtccgta cattgctact ccataacgaa tcatattgcc
    49021 gattgtttca tcatgccata aagccgttgc actattgctg acatgtacat atcgaggaag
    49081 ttcaggaagt ttttttagca cttctataaa gcgttctttt tgcagattcc aatatgtatc
    49141 atctgcctga tctgctgtag aaaaatgggt aaataaacct tcccattcta atacacgact
    49201 ttctttgatg aatgcagctg cctcttttac ttcttcaggt gtacaaaatc cgatacgacc
    49261 catcccagtg tctgctttta gatgaatcga aagaggcgtc tctagcttac ttttctctaa
    49321 aacagcagct gctgcctgca accattcccg tgtacctgca gttactgata aatcatgagc
    49381 aacagctaca gtcagtgcgt cgtaaggaac catattcaag ataaggatgg gctgagtaaa
    49441 tccagcttct ctcaactcga ctccttcatc taaatttgaa acacaaaagc ccgaagctcc
    49501 acctgcaact gctgcctcag ctgtttcaat agctccatga ccgtatccgt ttgcttttac
    49561 tactgcaaac aattcttttc cttgcggaag tcttgcaact tcatttgcta cattttcagt
    49621 tatagctttt ttatgtatca ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt
    49681 tccttctttc tagtcttcca aaataatttg tgcaaaagct gtttcatttg tatgagtaat
    49741 actgacccat acattgcctt catgaggtga acgcgtcaca acaggctgac cgttttcatt
    49801 ttttaaaatc tcgacatctt gaaaggtaac ttttccgatc ccagttcccc aagctttaga
    49861 gaaagcctct ttgcaagcgt aacgcccgcc taaaaattct acttggcgat gaaaaggcaa
    49921 ggactggaat agtttcattt catcagaggt caagattcgt gcaatgaatt ttggtttttc
    49981 ttcgatcagc cgagtgatcc taggaagttc gactgcatcg atcccaattc cttttatcat
    50041 atgcttttct cttttcaata aatttagaac actctcattc taccaaatta taaccatttt
    50101 gccacaaata agtccacaaa taacaaaaat gaaagtttct cttaaaaaag agaaactttc
    50161 attttatgtt taatcgttat ttgaacggat tacaaatccg cgttttttat cattgtgacg
    50221 tttaccgcca tctttttggc tgttattatt tttgctataa ccgccttttg atttgtttcc
    50281 acgataatta ccgccgtcac gacgattacg gttgtttccg ccaccacgtt tgccattttt
    50341 attgtatcct tttttctgca ttggcaatgg gcgttctggt gtgatcttca ctggcactgc
    50401 atcagctgga tcttttgctg tcgtttttag caaaagtgca actaaatctt gtgcagaata
    50461 ttcttctaat aatttatctg ctgtttgcag gtatttatct agaccatttt ctgcaagttt
    50521 tgtttcgatt tgttcaactg cagcacctaa ttgacctttg aatgcttctt tttcggttgg
    50581 tggacgtaaa gtcgtcatcc gttttttcgt caagttttca atcacatgca agtagtccat
    50641 ttcatttggt gtaacgaaag tcacagacat accgcctttt ccggcacggc ctgtacgtcc
    50701 gatacggtga acgtagcttt ctggatcttg agggatatca tagttgtaca catgagtgac
    50761 acctgaaata tctaatccac gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc
    50821 gctcttaaat gaacgcaata cgctcattcg tttttgttga gacaaatcgc catggattcc
    50881 ttctgcacgg taaccgcgtg cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt
    50941 acgtccgaag acgattgtta gttcaggtgt ttgaacatca aacaagcgag tcatgatatc
    51001 gaacttttcg tattcttttg cgcggacata gtattgatcg atcaagtcag ctgtcatttc
    51061 tttcgctttg atcttcacat gatgtggatt tttcatgaat ttaacgccaa tattcttgat
    51121 agctggcggc atcgttgctg agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat
    51181 gattttttcg atatcttcca agaagcccat attcaacatt tcatctgctt catccaaaac
    51241 taatgtttga acggttccaa gtttcaatgt gtggcggttg atgtggtcaa gcatacgtcc
    51301 tggtgtaccc acaacgatat gtggacgatc tttcaagcca cggatctgtc ggccaatatc
    51361 tgctccgccg taaacggctt gtacacgtac ttttttgtcg cgccctaaac gatataattc
    51421 ttcttgtgtt tggatagcta attcacgtgt tggtgcgata actaaacctt gtaattcgtg
    51481 acgatctgga tcgatttttt ctaacattgg taatccaaat gcagctgttt tccctgtacc
    51541 tgtttgtgct tgtccaataa cgtcttttcc agctaatgct agtggaatcg tttctgattg
    51601 gataggcgtt gcttcttcaa aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa
    51661 ttctagttct ttaaatttca aatgaatcct cctatatggt tgttaactga ggatgtagct
    51721 tttttgctac cccgtcgatt tgttcggtca aagtgtattg tttctttttg acagtggcca
    51781 cgcacgtttt tatccattct tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt
    51841 taggcattag aaaagccaaa cgggtcgcgt tcagctttat cattattatg acatctggct
    51901 tcaacaagtt gtttctatgg caaaaccagt ccaatttctc ttcatctttc ggcaataggt
    51961 tattctaaca tagtttttcg ttttctgcaa gtttacagat ttttatttca tttgctgaag
    52021 ctgttctacc acttcaaata agcccatgct gttgctgcct tttagcataa tactatctgt
    52081 tgcttcaaga ttttgtttga tagcatcaat caatttattc ttttcttttt caaaatagaa
    52141 cacaggtagt tgggggtatt tttgattcaa ttcttctttt aatgcatgca tttgttcccc
    52201 gtacaagaaa atcatggcaa atttctcagg atcgatatgt tcactcattt gcgcatgcat
    52261 gcttaaagaa tctttcccta attccagcat atctgctaac acagcaattc tgcgcccttt
    52321 tgttggcaga tttttaaaag tatctaatac cagtcccatg gctgttggat ttgcattata
    52381 gacatcgctc aaaatagctg caccatttcc tgccgttagc cattcggttc tgttttgagt
    52441 gatagaaaca tgagcgagcc cttggcgtat ctcaggtatg gacagtccaa agaagcgccc
    52501 gatcgtatac gcaatcaaag cattcgttac attataactt ccaggcagcg ggatcgtaaa
    52561 tgtttctcct tctactacaa aatcggtttg tgcttttcct tttgctgtca cttccgcatg
    52621 aacatcgcca tttccaatac cgaatgttgt gactgtttgt actaatgttg ccgttaaagg
    52681 tgtcagcaac ggttcatctg cgggcacaac gagtaatccg tctggtacaa gtccgtcagt
    52741 gatttccatt ttggctttag cgattccttc tcgcgaccca agattttcta tgtgtgcttc
    52801 accgatcatt gtgattgctg cagcttttgg ttgagccatc agtgaaagtt ccgtgatttc
    52861 attggcgtga tccatgccca tttccaaaac gatcttttct gtatcttggg gcatgtgcaa
    52921 aatcgtgtaa ggaagaccaa tattattgtt gtaatttcct tgcgtcttat atgttttgta
    52981 tttttcagac aagacagacg cggttagatc tttcgtggtg gttttaccat tgcttcctgt
    53041 aattgcaacg acttctgcgc ctactttttc caagtaatag gttgccaatt gctgcatggc
    53101 tttcaaagga tctgctacag gaataactgg aaaatctttt ggagcttgtt caacaggcaa
    53161 gctccaaaat gaagcagatg ctccgtttgt gatcgcttgc tggataaatt catgtccatc
    53221 tcgcgtacca gcaagaggaa cgaataaact tccttcggtt atcttccggc tgtcaaattc
    53281 gacattggta atctttgtca gtgcagagca ttcgctgccc acggcttgtg caatttcttg
    53341 tattgttaga ttcattgaga tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa
    53401 acggcgggtt cgcatgactc tcacttccaa gtaaatttct ctattcgtat ttagaagcta
    53461 gaatgaacgc ggttacgcaa tcactccagc ttcttcttta tttctcttta ttcatttttt
    53521 tcaaaaaaag attgacgctg atggtatcga ttcattccta attgaatcag ttcttcaatc
    53581 aaatcaccgt atttcaagcc catattttcc cataagagtg ggtacatact gaactctgta
    53641 aatcctggca tagagtttaa ttcattcagg aataattcat ttttatttgt caaaaagaaa
    53701 tcacaccggc tcaatccgct tccgcctaac atcgtgtaag ctaacttcgc gtactcttgt
    53761 gctttttgat aaacttcttc tggcacttcg gctggaatct gcatttcgat tttattattg
    53821 atatattttg cttcataatc atagaatgct acgtctttta cgacttcacc aggcaaagtc
    53881 gtccgaacgt cttcatttcc taatacagca acttcgattt cgcgcgcttc aattccttgt
    53941 tcaacgattg ctcgagaatc atactgatag gctgttgcta aagcattttg cagctcttct
    54001 cggttttctg cctttgtaat gccgacacta gaacccatat tcgcaggttt gacaaacatc
    54061 ggataaagca aagaaccttc acattgatca aatacttttt taggattttc tttccattga
    54121 ttcttaagta ctggtacata aggaacttgc ggcacaccag cagcttgtaa aatatacttg
    54181 gtcatgattt tatccatggc acatgcactg gtcaatacgc ctgcgccgac ataaggcata
    54241 ttcaatgtct ctaagaagcc ttggatcgtt ccatcttccc cgtttggccc atgtaaaact
    54301 ggaaaaacga tggctccttc ttctttgatt tcgcccggat tgatcacttt tcctgtaaag
    54361 ccttcctctg tctgtccact tgggtcccat gaaagatgca agacatcttt gctggcaggt
    54421 ttttctgtta gtaatggacc tttgacccat tgtccttctt ttgtaataaa tacgagttga
    54481 acttggtaat aattataata aatggcattt aaaactgaaa atgcggaaag aatggacact
    54541 tcatgctctg cgctgcgtcc gccatatagt aaagtaatct tcaaaagatt gtcctcctgt
    54601 attctaaatc gttcagtgat ttactctcat attttagcac aaaacaaaaa taaacgaata
    54661 acttatttat aaaaaaacga gttggtttct aaaagaacaa acgtcattta ttttttgtca
    54721 gctagattat ttgtcccgtt ttttataaaa agttatgata aagaaaagga gggatgatga
    54781 tggcgcaaac aaatccacgt agccgcttgg cagaaatcat tcgagtgttc atcaattaca
    54841 atgtagtgcc aaactttgta cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag
    54901 aactaggccc caccttcatc aagataggac agatgctttc tgttcgagaa gatttgcttt
    54961 cttctgcctt cactcaaaca ttcaaaacat tacaagatag tgtacctagc gacacatttt
    55021 caacggtcaa aaagacgatc gaaacagaac tttctctttc attatcagat atattcgatg
    55081 atttttcaaa atcgcctttt gcctctgctt ctatggggca ggctcatcgt gccaagctga
    55141 agaacggtga ctcggtcgtc gttaagatcc aacaccctaa catcgccgaa gagattcgac
    55201 ttgatcttca actatttgaa cgagcgatcc ctttgatcaa atacattcct gaaactagcg
    55261 tagttgatct aaaaggggta ctccaagaag taaaacgttc cttgatcaat gaaatggact
    55321 tcttaaaaga aagtcaaaat ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga
    55381 tccgcagccc caaaatttat gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa
    55441 tgtctggtaa aaacttgaat catttgatga atgccgaaaa cggaacagaa acttttatca
    55501 ccggtattca aaacaaacag ctgaaacaag aagttgcaaa gcttttagtt gagaacttta
    55561 tgaaacaggt atttgatgat ggttttttcc atgctgatcc tcatccaggg aatctgctat
    55621 tccatgtact aacaaaagaa gaacagacac gagctagcca aaaaactgaa acagtccatg
    55681 aaaaagagtt cggttccttt acttttcgag cctctacttc tacagaagac cctgttgcgc
    55741 catataccgt caactacatc gattttggta tgatggggca tctttctgca ggacttaggc
    55801 aaaaactcac acaagccgtt ttagcgctgt atacaaaaga tgcttatcga atcgaaaaag
    55861 cggtccttcg actttgtcag caagaaggct cgtttgatga aagtcggttc caccaagaac
    55921 tgacaagttt tcttgaacag tattatgatt ctcctattga tgaaatcaac ttgcaagaag
    55981 tattcacaca cgtcgtgacg atttgtcatc aaaataatct gcagttcgat cgtgatatca
    56041 cgcttttgct gaaagcattt gggacattag aaggggtgat ccgcgtcctt gaccctgaag
    56101 tttccctcat ggaagtcgct tcgccttttg cccaacatta ttttctcact catctggatg
    56161 tagaagatac attgaaacaa agcggattag acttattgga agggatgaag gctgcgccta
    56221 agatccctca acagctccac catctattag aaatgtggac tttgggacaa ggaaaagtca
    56281 atttggaatt gaaaaagcaa gacaaattat tgagcaggat cgaaagtatg atcaatcggc
    56341 ttgttttcgg tatgatttta gctgcattga tcgtagggtc ctccctgctt gtccaagcag
    56401 cacctgtaga aaacgcagag gtcgtttcct tattagggat atttacgtat gctattgccg
    56461 cattcgtcat tatttttttg gcaatcgatg cattgatcca aatgtataag aagaggaaaa
    56521 agtagaaaaa ggttgtgaca gaaagaatca gcttcaagaa ataagaaggg attttgcata
    56581 aattgtttct caaatttttg tgaaaattcg gcttattttt cgaagaggct acttctgaag
    56641 caccgagaat aaaaaatgtg aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa
    56701 ggaacatgga acacatttat cctgtttccc tgttcccttt ttgatgtatt cgagcataaa
    56761 taaaatacct tcgttaccta ctatcttctg tacttggtaa accttggtca attgcctttt
    56821 ttaacgcttg acgaaaagac atatgatctt tctccgtaaa gggttttgga ccctttgtcc
    56881 ttatgccagc ttttcttaac tttccactat gataacgttg ttctagaagc gtgccgatat
    56941 tcgcttcgtc aaattctttt ccagagtgat ggaacactcg cacacccttc ggcaatacaa
    57001 gtgaagctaa accataatct tgcgtgatca aaaagtcatt cttcttaacc ataccgacga
    57061 ttttataatc cgcactatca gctccgcggt ctacgtagac gatttggaca tgattagcgt
    57121 attctttggt cgtataatgg tccacactgg ttacgataat cacgtcgaga ccataggatt
    57181 ctcctaattt tatgacatct tcttttactg gagaaccatc tccatcaatg aatagcctca
    57241 ttctttttcg ccttcttcca taaatgataa acagttacta atccgctcag taagagacca
    57301 acattgatcc attttgccaa gataagcagt tgtacacttt ctatcgacac tgattcatgg
    57361 ttcagcgtga tcaataatag atggattggc tctcccattt cttttgctaa aataaacaaa
    57421 agcgtcatca ataaaaactg gtggatcaca ctccctttat tttgagtagt atacttgaca
    57481 acgatccggc gatccattgg aatagctgca tcagtttgat aatattcctt atttagccag
    57541 ctgcctgtta ctacgtgcca gaaactaagc agaaagacac ctatcgcaaa accatttgga
    57601 atcaagagga ccaataaaga agaactcagt ccaagcaatt gcatagccaa tggagaccat
    57661 gataaacgtt tcaaaatcca tggtccgact atttttgctg caatcaagcc tgctgcataa
    57721 ggcaaataca taaaaatcga aagtttttct aatccgtaca gtatgccaac ataaaaactg
    57781 ctaaacaaaa aaaggaaatt ccctagcatt ccatttagaa aagtaaacag atttagccag
    57841 cttggcaact tccattcctt cttctggtgt cctaaaacaa acacgaacaa gatgaacagc
    57901 agcaagaaac cataaatagc aagatcaaaa agcctttcat cgaataacaa tcgggcattt
    57961 ctcaaaagaa acaataagcc aaagaatatc aaaaataaaa caagctctcg cgtagctacc
    58021 agatttcttt tgatctcttt gaaatccagt tcgtaacgcg gataatgact aaccgtatgg
    58081 taggccataa cgtaaaaaag tgtataaata gtaaagcttg ctgcaatctg agtcggtgca
    58141 ggcaactcaa tcgcttgata caggagaaat agccagatag ctgcaaataa gtactttttc
    58201 ccatccatat tgataaaacc ttgcgtcttt tcgtgaaaat tgaccgtggt attagcaggt
    58261 acaagccatg ctgcactcag ccctagcaaa acacttgaaa aataaaataa aggaaaataa
    58321 aatacgccta atattccaag caatgaacca gcaccaccaa tcagcagagc gatcatcagc
    58381 aacgtgtaac tatctagacc aaatcgtaga ctgttgacta aaaatagtcc agtcatccga
    58441 aaggcataaa acagcgtaaa tggcaaaatc gtcagccaat tatgcgagtc tgttagtccc
    58501 aaaaataaaa gataaggaat aaaaataacc gtgttactta agataaaggg cgttgtttgt
    58561 gtaaaattac gcccaaatcg tttgagtttt tcgaacatga caatggctcc cttaaacagt
    58621 cttataacta atctgctctt cgattttagc attaaagggg aaataaacca attttacaga
    58681 atatattctt gtaaaaaaaa atgcagaaag aagcataagc ttctttctgc atttttacgg
    58741 tacttgatta ctcaagcaga caagcccttc tccacaaaag tcttgcccga cacccctgca
    58801 tccggcaggt gataaagata gaaatcaatt atcctgtgcc tccaaaatca attaggagct
    58861 ctgtaaacca gagtccttct cgacccgtcg cataaattta ctttaacaca gttcctatgt
    58921 ttatgcgaga gcttttttca ttttataatc gaccatttga aatccttgac cgatatctga
    58981 aacaagttct tctgtaatct caaatccttc atgcttataa acctcgattg cttgatgatt
    59041 tccctggttc actcgcaaaa agatctctct ttcctttgtt tgcgcagcaa gttcttcata
    59101 ccagcgaaaa actgaagaag tcaatccttg accacgtact ttatttagca aatataattt
    59161 acttaaataa atttttccat ttatctcttc ataagcagtg taaccgaccg tcttttcttc
    59221 gctttttaat aaaaagtagg acacaccttc tgtcagttct ttttgaatct gtttttttga
    59281 ctgataagtt ttcagcatgt aatccacttg tttttgacca atgatcggca caaaagcttc
    59341 cggccatact tcttgtatga ctgccatcag ctcatccaat tcattttctg ttacttttat
    59401 tttttccata cacccacgtc cttttttaca ttataaatga ccttgcgctt gatggaataa
    59461 aaagagcatg atcatgctat acacacctac cgaccatggt ttcaagaaaa tcacaatctg
    59521 cataaattcc ctaaaagata atttcgtcaa accagctact agacagatca agtcgtctgg
    59581 cgcaaaggga agcagcaagg taacgactag tacttttttg atatctttcg tttttcttgt
    59641 cagcaattca tcaaatttct gatacttttt tggcgataaa ataagctgga caaaggctac
    59701 gccatagtaa cggacaagta aaaatccgat tgcttcccca atcactaggc caatacaact
    59761 atataaaagc ccaaatccat tgccaaacat cagcatgccc gctactgagg agatccctcc
    59821 cggcaaaata ggaatgatca cttggatcac ttgcagcaga acaaatatca tcactgcata
    59881 cttgccaaat tgctggataa acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg
    59941 ataagcataa agaatcaaca caaagaaaaa caacagacca ataatgggca tgatctgaag
    60001 acatttttgt aaaactgatc cttttttaga catctatatt ccccacatct tttttcattt
    60061 attaaaactg cctcct
//
LOCUS       DACCKU010000005        59472 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.005, whole genome shotgun sequence.
ACCESSION   DACCKU010000005 DACCKU010000000
VERSION     DACCKU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 59472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59472
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.005"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            92..2737
                     /gene="polA"
                     /locus_tag="IVN13_000282"
     CDS             92..2737
                     /gene="polA"
                     /locus_tag="IVN13_000282"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP7010804.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            2782..3618
                     /gene="mutM"
                     /locus_tag="IVN13_000283"
     CDS             2782..3618
                     /gene="mutM"
                     /locus_tag="IVN13_000283"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP7010805.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            3582..4211
                     /locus_tag="IVN13_000284"
     CDS             3582..4211
                     /locus_tag="IVN13_000284"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP7010806.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            4263..4595
                     /locus_tag="IVN13_000285"
     CDS             4263..4595
                     /locus_tag="IVN13_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7010807.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            4907..5398
                     /gene="nrdR"
                     /locus_tag="IVN13_000286"
     CDS             4907..5398
                     /gene="nrdR"
                     /locus_tag="IVN13_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP7010808.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            5421..6794
                     /locus_tag="IVN13_000287"
     CDS             5421..6794
                     /locus_tag="IVN13_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP7010809.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            6794..7720
                     /gene="dnaI"
                     /locus_tag="IVN13_000288"
     CDS             6794..7720
                     /gene="dnaI"
                     /locus_tag="IVN13_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP7010810.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            7849..8289
                     /locus_tag="IVN13_000289"
     CDS             7849..8289
                     /locus_tag="IVN13_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP7010811.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            8433..9134
                     /locus_tag="IVN13_000290"
     CDS             8433..9134
                     /locus_tag="IVN13_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP7010812.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            9131..10252
                     /locus_tag="IVN13_000291"
     CDS             9131..10252
                     /locus_tag="IVN13_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP7010813.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            10267..11499
                     /gene="pepT"
                     /locus_tag="IVN13_000292"
     CDS             10267..11499
                     /gene="pepT"
                     /locus_tag="IVN13_000292"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP7010814.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            11631..12539
                     /locus_tag="IVN13_000293"
     CDS             11631..12539
                     /locus_tag="IVN13_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP7010815.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            12574..12846
                     /locus_tag="IVN13_000294"
     CDS             12574..12846
                     /locus_tag="IVN13_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP7010816.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            12857..13903
                     /locus_tag="IVN13_000295"
     CDS             12857..13903
                     /locus_tag="IVN13_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP7010817.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            14154..16763
                     /gene="clpB"
                     /locus_tag="IVN13_000296"
     CDS             14154..16763
                     /gene="clpB"
                     /locus_tag="IVN13_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP7010818.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(16914..17585)
                     /locus_tag="IVN13_000297"
     CDS             complement(16914..17585)
                     /locus_tag="IVN13_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010819.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(17578..18072)
                     /locus_tag="IVN13_000298"
     CDS             complement(17578..18072)
                     /locus_tag="IVN13_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7010820.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(18092..19312)
                     /locus_tag="IVN13_000299"
     CDS             complement(18092..19312)
                     /locus_tag="IVN13_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7010821.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            19459..21294
                     /gene="lepA"
                     /locus_tag="IVN13_000300"
     CDS             19459..21294
                     /gene="lepA"
                     /locus_tag="IVN13_000300"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP7010822.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEQDSVK"
     gene            21490..22401
                     /locus_tag="IVN13_000301"
     CDS             21490..22401
                     /locus_tag="IVN13_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7010823.1"
                     /translation="MVDNSSGRVLKSLRKEKKLSQKKLADLAGISQSTLVKYEKGSRK
                     IPKDVDNTLSKILNIETLIKDEEDKIEILIGKLIAYRDMNKLLNKELADNIGISEVLL
                     SYVLNRKRNPSKEMQKKIDIFLLSNEKEILKEINRDSEIFSLSKDDKIVMGKRIREVR
                     KNREETLEKFGKNFTIYTGKNVISRWEKGINIPDIEKLMNIAYLGKVTVPYLMYGEDY
                     KNILPKDERVSDFKKINSFSMGLRMRKIRKDYYLEREEFGKLFSPSISKWSIDRYENG
                     RDIPNTNRIIQYAYIGNLSLEFLIYGI"
     gene            23397..23648
                     /locus_tag="IVN13_000302"
     CDS             23397..23648
                     /locus_tag="IVN13_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010824.1"
                     /translation="MRKVLVFNTRGQSFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
     gene            23731..24003
                     /locus_tag="IVN13_000303"
     CDS             23731..24003
                     /locus_tag="IVN13_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010825.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            24093..24332
                     /locus_tag="IVN13_000304"
     CDS             24093..24332
                     /locus_tag="IVN13_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010826.1"
                     /translation="MIDISLPEDLKPDPVTIGNSVMIFDSLDEAMSYGWKEIKDEGSN
                     WYGMRFGDGEVAKKAGIPIGKWTVDFHEAGSFFVL"
     gene            24479..28762
                     /locus_tag="IVN13_000305"
     CDS             24479..28762
                     /locus_tag="IVN13_000305"
                     /inference="COORDINATES: protein
                     motif:HMM:NF019941.1,HMM:NF037291.1,HMM:NF037639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HAP7010827.1"
                     /translation="MYAKTKMNWLKALALVAFSQFCFLVGTAQAHAEETTAIQPTMEA
                     IAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKTTLDQTIGETQHPGNSPGT
                     SQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQAEEKKRIEETVEQPAATA
                     DKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKKKEQNIPKTGTPTLLVTVP
                     TNRSVRVRRATNAAVLGGRKIDWGENIGVTSSPNNLNFTLNYMVRFEINGQVAFCIEP
                     GVSAAEGQVYTATALKNYLKNSNVRKKTSLISYLGYVTNKDKSDEQYIAAQFMIWEAL
                     GTKIPIKNIRVDYDRRKAEIQRLINNFGEKATFNNQTHTVKIGETLKVKNKNDFLKYV
                     KDIQKPKGVNVSIHGNEVWITVTKEAPDKAKIQLNKIKVQGAPLAYRRPGAQTIGVLH
                     PTEPNASVINLQILKQGNIRVFKEDAETGVKAQGAASLENAVYGLYQADGKKIKEITL
                     KKINGKVQAEIKDLDPGKYVLKEIKAPKGYVLSDEAIEIVVEPGKTVNATAKDQVIKG
                     TIDLVKVANKDLVDSTNPDNKPKLAGIEITLTSKTTGKVVKTVVTDNDGYATFGKNTV
                     VFDTYILSETKGKEGYKLFEPLEVTISENGQTFHYVLEDKVIEQRLKVVKVDQETGKV
                     IPLANTEFKIFDTLANRYVTMEIPNDTETTDIFKTNEKGFFLTNGTLTYGKDRYRLEE
                     IKAPVNYVLNQNPLVFSITNDTEPIKVIHFANRQARKSVKLFKYEEWNKKKTPLAGVN
                     FTLYNGKNQAIGEYTTDQNGEILVKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQ
                     NAPDDGETLVVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKE
                     YTISGVLMNKATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFE
                     KLFRDGIEVAAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITE
                     ILPGKAYLVKGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGR
                     EIVVFESLFRNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTIT
                     DAVKYHDLVVGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILD
                     ARALRGKEVVVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILAL
                     PNQTVLEWVKVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFL
                     VDGRNLAGKGLSFAEWLQAKTEDEKETFEEVAKHNVDLTNKDQTVQIVEAPKPPQPVK
                     PEQRVKPATVVPMLPKTGEQTSSILMVLGLVVLVIALGITLYKVRKAKD"
     gene            28861..29226
                     /locus_tag="IVN13_000306"
     CDS             28861..29226
                     /locus_tag="IVN13_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010828.1"
                     /translation="MAEKALRMKKEFLGQVFLLGSREPNPPKEINGVSGRYVQLQAEC
                     SREEQPAFFVYLTAEEDKRLRLPEKEVRISFEEAYFVFRKERVNSRETNDMGQTERRT
                     TGYQDVGVFLTGVKIGEVL"
     gene            29228..29680
                     /locus_tag="IVN13_000307"
     CDS             29228..29680
                     /locus_tag="IVN13_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010829.1"
                     /translation="MGLKFPNNYDITEALDETCLGAVRFGALEESDRVFDNETGDYTG
                     EIRSQRAQLVFGNPDEPKIARRVKFPADVDLTGFKRRDPVKLIDPEYHVVYIPAQDGN
                     REYSDLVIYAKGLEKVEAPTNNPNMKQSQEKGLDQKQPSVDSKEKEKK"
     gene            30294..31604
                     /locus_tag="IVN13_000308"
     CDS             30294..31604
                     /locus_tag="IVN13_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7010830.1"
                     /translation="MYLIPGISIVLYLLLVSGLILGLMHWSRLKEGYFASVYWRQLLV
                     RMLIDNRFVYTKKQVSEKQTREKMRLPKVYFYQKKQELVVSFPLDGGRFHERFLKMGH
                     LLSEVFLRDLIREEREKARINYVFLSDSGRIPIDQCIAENGRIHLIKTLDWVYDQSPN
                     MLIAGAIGGGKTYLLYSLIQACLSVGTVDICDGKAADLAALGDVDVFKGHVFYKINEE
                     MIICLRNALKEMNQRYAYMKTMKDPKYEPGKNYAYYGLPPHFIFFDEWAAFYGSLDME
                     ARNRVDKLIQPLVLKGRQAGMYIILAMQRPDAEYFPSGVRDNLTFRISVGRLSPIGYL
                     MTFGEDFKNKPFYNKDQPGRGYAFPGFDVPVEFFAPFVYKDYHFIKEFEKFTEMIVYD
                     FTVKEDSQDQTMGDDNQLLQEQLERQMYLAKDPKGTSFSDAHIL"
     gene            31627..31938
                     /locus_tag="IVN13_000309"
     CDS             31627..31938
                     /locus_tag="IVN13_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010831.1"
                     /translation="MAESCYYRVATAIRMINPSLSSRTFYDWLNRIEQVTDYRFLRKE
                     RVFTGKVINQVLLTKKDIERLTRLYHYRVDLEEDLTLSIYRVFSPEKYSEITKLDHLI
                     L"
     gene            32299..33555
                     /locus_tag="IVN13_000310"
     CDS             32299..33555
                     /locus_tag="IVN13_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7010832.1"
                     /translation="MTPIELKSYRKKLSAIHNISGDKKPLSQARFAQLMGCSKDYIRK
                     MEQGKRKIPEDFEQRIKERFRLYMKSGIDLRAMIDYLRLSFFGTDYKTVIQRVLRMDL
                     TEFETRPTGLYLFDCLSVRGNIWVFWHSKRTVTENVLVQFSGQGCREYEEVMREQQTD
                     WRTWLYSIWKNQGKLEAGYTRVQCSRFDIALDELWGLETEEHFDLFELLEKQKAGLLE
                     MDFKRFQNIGGCFLDDGYFRSEGLSLYFGSRKSPLFFNFYEKRFEIANREKISELEAL
                     TKYGIYNRYELRLANEKATQAVEAFILGDSPKRLGEIGVGLINAKLIVWDRLESDARI
                     VDKRWAKLFRSMEELDFTVKPEKFSLDRSERWFLKQVAPTLKLLQEADRILGRDFVQE
                     GIDNAELNDEQQNYLEWMGTSGGKIA"
     gene            33552..34097
                     /locus_tag="IVN13_000311"
     CDS             33552..34097
                     /locus_tag="IVN13_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010833.1"
                     /translation="MNAYTTTEWLRLLDLKAAIEALNEKMVDLSYFRFRVPYIEQAVK
                     AGRYQEKENWQEIARLLEVRKGYEQELEELEFSRRKGRLEFIRFYRFSLPIPAILAVK
                     KGCDKMKIYENCVAALSSEKPLMEEISLVTVTWEMSRQPTEEQTYLSLEEIEIELEEI
                     GRYATCSTYCGSVISIAGVIV"
     gene            34098..34598
                     /locus_tag="IVN13_000312"
     CDS             34098..34598
                     /locus_tag="IVN13_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010834.1"
                     /translation="MKKIMIYVGAYWSRDPTVLENPEAVHYCLRQLFYLYKERLESLI
                     RQLPYTDRRLDELLLRYPAMYKRRKNRLLPEEYPIEKHELEGRFVAYFYDDVRMRLVE
                     QRMEIENDRYYFINYCKKRKYQVTDDFYDCILQDGEVILSKIAPSFRELVPIDFLKKC
                     HLRILP"
     gene            34679..35235
                     /locus_tag="IVN13_000313"
                     /pseudo
     CDS             34679..35235
                     /locus_tag="IVN13_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353559.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35255..35599
                     /locus_tag="IVN13_000314"
     CDS             35255..35599
                     /locus_tag="IVN13_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010835.1"
                     /translation="MWRLLFLVVCGMALFYVYRVYLYPRMERWTSWYRDPLKQRKGLK
                     RYRYELLVEFSDGRQAQYYFFANYPKYNTSELLIQLFKNNPYVGLEENGREFFYRSDN
                     ISAASMNKTRIA"
     gene            35618..36529
                     /locus_tag="IVN13_000315"
     CDS             35618..36529
                     /locus_tag="IVN13_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7010836.1"
                     /translation="MKKTTLAVVTTLGISLFSFQLGSSLVYADETVETPKSEIVETLP
                     GEGLPDVETELNGGTTEKPAEPEQLQSEKPEKEPVTPEPEFPESTLPTDSIEEEGVHS
                     STTTDSSIVTTPEQSGTTSSSEKNTDSSSTEQPTMSSTSEEQKEEPKQPVEKSEAIPK
                     ENKPIVPAKEVTVSVTPSGEITTNTSQGMSVPIVTSNVEELTHIPTPTTPLKVAGDQT
                     IVGVKDGIPLIQDSEGNLVKDLSIPVKKLPSGNIEIKTADGKTKVLPKTGEEIHVALS
                     VLGGVLTSIAGFVGYKRKRKGIENEGN"
     gene            36608..37600
                     /locus_tag="IVN13_000316"
     CDS             36608..37600
                     /locus_tag="IVN13_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7010837.1"
                     /translation="MKKRQLRRKEKVYTEKVPKQKKIHSKKAVKWIWCLVLFLCFSGG
                     LAFFQSMKTKVQVSFLKDEIQQLQKKEVQSRSELTYTPEIESFMNRFVALYMNTFDNH
                     EQEQQRQETLKKEFYAQNFIQEPGSVSGVRKLTAASFIGLKEIETIPTASYKVTYDIR
                     SMNPESKKETSVTITQVLNLPFSISNKGCRIIAYPYFTALPKNTEKQRMITYDDTLYE
                     TVDASEAKDVEGFVEDFLDKYAKNNAKDMSYMMKEPEGLNQTAKVDQVEMKVLRYKET
                     KKPFIVKATVVFRDGDTSLLQKEQMTIVITKKTNQYYVEKLTHNWLDRKGEERK"
     gene            37597..37815
                     /locus_tag="IVN13_000317"
     CDS             37597..37815
                     /locus_tag="IVN13_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010838.1"
                     /translation="MTLKGIVDFILTQGGYFLIMVVVYLGLRNFMKSKVGNALISIAA
                     GAAVYFFLNDPTRVFDAFGKIIEKLFMD"
     gene            37828..38238
                     /locus_tag="IVN13_000318"
     CDS             37828..38238
                     /locus_tag="IVN13_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010839.1"
                     /translation="MDWEPYDYSIVYAEPYRVQQITSNWRLPFPVALVNAATFLLVVI
                     FEYLFLRSFIGWVISFFPPAGVVFYIGLPYLCVLALAKLKPDGKKVHFYLWDWFCYMI
                     RMMNGDCVYYNWDKVSNNALEELLFVNKYVYLED"
     gene            38290..40818
                     /locus_tag="IVN13_000319"
     CDS             38290..40818
                     /locus_tag="IVN13_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7010840.1"
                     /translation="MVLVKEPVKAFKKNCLLNGEDCIWAYYHVFPRTISKNTPKKTQE
                     IKEMMARLLLLLADYEDVELVMYPRTMNLRTKFDTLGKGFSPDSQAIGAYYAKETVTL
                     LEREGQVYRPSFILGIKVKEKATVESFHDVINLIWLDLAGSLARLFGLEVDDLETLFS
                     ESDPYVKEVTQLLYSFSIRPLVKKELLYLNRLQYIRGMAHKVEEQNNWEVLKDNQGTY
                     VRIPKEHKGLLQLEDELGSSYLALLPIGETPENMRNCDLFQFAQDLSFPAELRMKIHY
                     PRLKGQGGIGLKLNWLSERFKTEHKELVQNDEEPSERLLTVRGLVKHLKQRVDNGQPV
                     VDWIACFIVYGQTVEEVRQNSATVIQQLHTIIHIHRAKNDQRELFYKCLLGQPLGRQL
                     HWKHRTTVEAVGEWLFSAVSELGMESGWYIGRQDTLGDGGSRGEKTPLEELIYASNRF
                     VFLNLLAIAEGIKGALFDAPHVAVTGKTGKGKTFLVGLLFLYSSFMNVQSLFVDPKGE
                     KRKWFTKLIQDPYYQKHYPLFVEHLQKMSYVTLDATNPRNYGVLDPIVCLSRVDAKQV
                     AQDMIDELSPLGTNHLLKGAVLKGIQEVLVQKEAGQQVGLMHVVDYLEQMEQKEVREY
                     GEFLRLTVEDSILRLGFSYGENPGLDFNAKTTILEIQDLKLPNDNVRPELYTDADRKS
                     LCLMISLGRFCEMFGKRDSSKKTAIYFTEAWVFNNSNAGRSIIAAMARVGRSQMNQLV
                     LDTQFIGDLGSDKEKGNFGVVFAFDEDSEREKILNHLRLEVNEANLAEMSHMIKGQCW
                     MLDPYGRVGKLNVHSLFEEMTAALQTTEKTSHSQVEEQDTLIMQ"
     gene            40834..43044
                     /locus_tag="IVN13_000320"
     CDS             40834..43044
                     /locus_tag="IVN13_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010841.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            43052..44143
                     /locus_tag="IVN13_000321"
     CDS             43052..44143
                     /locus_tag="IVN13_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7010842.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            44164..44706
                     /locus_tag="IVN13_000322"
     CDS             44164..44706
                     /locus_tag="IVN13_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010843.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            44928..45158
                     /locus_tag="IVN13_000323"
     CDS             44928..45158
                     /locus_tag="IVN13_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010844.1"
                     /translation="MCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQKGYPYGPTN
                     LKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            45172..45504
                     /locus_tag="IVN13_000324"
     CDS             45172..45504
                     /locus_tag="IVN13_000324"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010845.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            45549..46097
                     /locus_tag="IVN13_000325"
     CDS             45549..46097
                     /locus_tag="IVN13_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7010846.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            46104..46556
                     /locus_tag="IVN13_000326"
     CDS             46104..46556
                     /locus_tag="IVN13_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010847.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            46653..46988
                     /locus_tag="IVN13_000327"
                     /pseudo
     CDS             46653..46988
                     /locus_tag="IVN13_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(47713..48384)
                     /locus_tag="IVN13_000328"
     CDS             complement(47713..48384)
                     /locus_tag="IVN13_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7010848.1"
                     /translation="MTIKTSLQNQAYQSIRRQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRQQGLVDTIPQSGNYISLIDLDSAKNARFVREHLERQIMLECCAKMNNQ
                     KKRILETIISEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLENNNTHLERF
                     RWLRVKTEGLKWEKIMEQHYQLFEALTKQDPEEANFLTSLHLHLMLNEQNIVLATHPK
                     YFKNI"
     gene            48688..50319
                     /locus_tag="IVN13_000329"
     CDS             48688..50319
                     /locus_tag="IVN13_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP7010849.1"
                     /translation="MVSIKNWQNSIEEFNEMQILLPCFDVKEVKQEGQQHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELETGVVVCETFDEEVVEKAYHDYNNDILEIVMSGDGEL
                     KKRLLAVTADSLYCNPKNIADYQRVVKYFEDPKLQFTTFTITEKGYSLKDSKGQFFSV
                     VKADIKSGPSNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFTIAE
                     AWQKEGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSENVKEDLLMMGLEDLDIIHTK
                     KHTNIAAFTNTEVVHYLVIEDSFPNGRPKLEKAGVIMTDRETVDKADIMKVTTSLNPL
                     HTALAVTGCLLGYQSISAEMQDDDLVGLIKGIGYLEGLPVVENPGIIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGENFVPSPDPLLVELQDKLSVLKLGEQDPSKIHCAVQEILENKEIFGLDLYQ
                     VGVGKKTEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            50322..51401
                     /gene="uxuA"
                     /locus_tag="IVN13_000330"
     CDS             50322..51401
                     /gene="uxuA"
                     /locus_tag="IVN13_000330"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7010850.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKSLKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLDWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            51475..52902
                     /gene="uxaC"
                     /locus_tag="IVN13_000331"
     CDS             51475..52902
                     /gene="uxaC"
                     /locus_tag="IVN13_000331"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7010851.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            52986..53633
                     /gene="eda"
                     /locus_tag="IVN13_000332"
     CDS             52986..53633
                     /gene="eda"
                     /locus_tag="IVN13_000332"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP7010852.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            53753..55129
                     /locus_tag="IVN13_000333"
     CDS             53753..55129
                     /locus_tag="IVN13_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7010853.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            55139..55933
                     /locus_tag="IVN13_000334"
     CDS             55139..55933
                     /locus_tag="IVN13_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7010854.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            55946..57712
                     /locus_tag="IVN13_000335"
     CDS             55946..57712
                     /locus_tag="IVN13_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP7010855.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            57735..>59472
                     /gene="uidA"
                     /locus_tag="IVN13_000336"
     CDS             57735..>59472
                     /gene="uidA"
                     /locus_tag="IVN13_000336"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP7010856.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDR"
BASE COUNT        20447 a         9117 c        12521 g        17387 t
ORIGIN      
        1 aattatttgg tatggtcccg ctttttcttt tcgcattccg caggattctt tgttagaatg
       61 aataagaact ttacagtaag gagttaagaa gatgacaaaa aataaattat tactcgttga
      121 tgggaacagt gtggcttttc gcgcattctt tgcgcttcac aattctcttg aacgtttcaa
      181 aaatagaaac ggtcttcata caaatgcaat ctatgcattc aatacgatgt ttgaaaatgt
      241 tatgcaaaaa gagcaaccga ctcatgttct ggttgcgttc gatgcaggaa aaacgacttt
      301 tcggacagaa atgtatgcgg agtacaaagg tggaagatcc aaaacacctg gcgaattcaa
      361 agaacaaatg ccatatatac gtgatttgtt gacaggacta ggggtacagt attacgaatt
      421 gcccaattat gaagcagatg atattatcgg tactttggca gaaaaagtag acaaagatca
      481 atttgatgtc gtcgtgcttt caggtgatcg ggatttgact caattagcta gtaaagaagt
      541 aaaagtggat atcactgtaa aaggtgtcag cgatatcgaa agctatacac cagaacatgt
      601 agcggaaaaa tatgatgggt tgacgccaaa acagattatt gacatgaagg gactagccgg
      661 agatgcctct gacaatatcc ccggtgtgac taaaatcggt gaaaaaacag ctatcaagct
      721 attgaaacaa tatggctcag tcgaaggaat ttatgaacat atcgatgaaa tgaagcaaag
      781 caaaatgaaa gaaaatctga taaatgacaa agagcaggct ttcttatcga aaaaattggc
      841 gaccatcaat acttcagcgc ctgtggatgt ctctatcgac tcattgaagt atgaagggaa
      901 aaatctcgat aaactcgttc cgttttataa agaaatggat ttcaatcagt tcctttctaa
      961 attgaatatc gtagaagaag aagtaaaaat ggatgatatt ttgtttgaag tcgttcatga
     1021 attcaaagag gaaatgttta ctacggatat ggcgttgtac gtggaaatga tgggcgacaa
     1081 ttatcataca gaggaaatcg tcggagtagc gtgggggaca gaaaagaaaa tttatgtcac
     1141 gaacgatcta tctgtattcg aaaaccgagc gttccattct tggatcactg atccgactcg
     1201 gctgaaaaaa gtatatgatg cgaaacgaac ttatgttgct ttaaaccgct atactggaaa
     1261 aacgaaagga atcgattttg atgtcttgct aggtgcatac cttctcgata cgaatgaaaa
     1321 aagcacagat atcgaaggaa tagctgctca ttacggatac aacgatatcc aatcagatga
     1381 agctgtatac ggcaaagggg cgaaaaaagg tttaccagaa gaagaggaac tcttttttgc
     1441 tcatttagcg cgaaaagtag ctgcaatcaa tgcgttgaca ccaaaattga gccaagaact
     1501 ggcagaaaaa aaccaagaag atcttttccg gaaaatggaa ctgccattgt ctatcatttt
     1561 ggcagagatg gaaataagcg ggatcaaagt agatgcgact cgcttacaag aaatgaaggg
     1621 cgagttctct gctcgcttaa gagagatcga acaaaaaatc tacgaagaag ccggcgaaga
     1681 attcaatttg aattcaccaa agcaattagg agtgatctta tttgaaaaaa tgggattacc
     1741 cgttatcaaa aaaacaaaaa ctggatattc cacagccgta gatgtactgg aacagcttcg
     1801 tgaacaagca ccaatcgtag aggatatcct cacctatcgt caaattgcaa aaattcaatc
     1861 tacctatgtc gagggactgc tgaaagtgat ccaatcagat ggaaaagtgc atacccgtta
     1921 tgtccagaca ttgacccaaa caggacgatt aagctctgtt gatcctaatt tgcagaacat
     1981 cccgattcga ttagatgaag ggagaaagat acgtcaggca tttgttccta gagaaaaaga
     2041 ctggctgatt tattcttctg actattcaca aatcgagtta cgcgtacttg cgcatatctc
     2101 aaatgatgaa catttgaaag ctgccttcct agaaggtcaa gatatccatg ccagcacagc
     2161 gatgcgtgta tttggaatcg aaaaggcaga ggatgtcaca ccaaatatgc gtaggcaggc
     2221 aaaagcagtg aattttggta tcgtgtatgg catctctgat tacggacttt cacaaaatct
     2281 aggaatcagc cgaaaagcag cacagcaata catcgatacg tactttgaaa aatacccagg
     2341 tgtcaaagaa tatatggaaa gaatcgtacg cgaagcaaaa gaccaaggct acgtcgaaac
     2401 gctgtatcac cgccgtcgat atctgccaga catcaattca agaaattaca atatccgttc
     2461 ttttgcggaa cgaacagcta tcaatacacc aatccaagga agtgctgctg atattttgaa
     2521 aatcgcgatg atcgaattgg ataaacgatt aaaagaaaca ggtcttcaag ctacgatgct
     2581 gttacaagtt catgatgaac ttgtcttcga ggtaccggaa aaagaacttg aatcattaga
     2641 taaattggtg aaggaagtta tggaacaagc tgtttctctt catgtaccac tgatcactga
     2701 cagcagttgg ggaaaaacgt ggtacgaagc aaaataaaca ttgtttaaga cagagcaata
     2761 taatgaaggt gaaaagaaaa aatgccagaa ttaccagaag tagaaaccgt tcgtaaagga
     2821 ttggaacggt tagtagtagg aaaaacgatt caaaaagttc aggtcttgtg gcccaagatc
     2881 atcgagcaac cagagacacc catctttgag gcttctcttg ttggcgaaac catccaatcc
     2941 attggacgta gagggaagtt tttaattttt catctcgatc attgtgaact gatctcacac
     3001 ttgcgaatgg aaggaaaata tcaatttacg aaagaaaata caccaatcga taaacatacg
     3061 catgtacttt tcttttttga ggatggaagc caacttcgct acaacgatgt acgaaaattt
     3121 ggtcgtatga caatcgtcga aaaaggagca agtgcaactt accgaggaat catgaaattg
     3181 ggacctgagc ctttgccaga tagcttctta ttagcagatt tcgcaaatgg actgaaaaag
     3241 tcccacaaag caatcaagcc attgctgcta gaccaacgtc tagtgactgg tctaggtaat
     3301 atttatgtag atgaagcatt atgggaagcg aagatccatc cagaacaacc ggcgaacaca
     3361 ctgcgcccca aagaggtaga acaattacgt ctatcgatca ttgatgtttt ggatcgtgca
     3421 atagaagccg gaggtaccac gattcgcagt tatttgaatg cattaggaga atcaggcgga
     3481 tttcaagtag cactgcatgt ctatcagcaa actggcaaac catgtatacg ttgtggaacg
     3541 ccgatcgtga aaacaaaagt ggcacaaagg gggacgcact attgtccgaa ttgtcaaagg
     3601 ctaaaaatcg caaagtagga tttgttttag gtttgacggg tggaatagct accggtaaaa
     3661 gtacggcagc caaagtcttt cagtctcatg gatttccttt gatcgatggc gatgtgattg
     3721 ccagagaaac tgttgagcct ggaacgcttg ctctgaggaa aattgtatca gtgtttggcc
     3781 aagaggtatt gcagcctgat gggcaactag atcgagggaa acttgggatg attgtttttc
     3841 catcaaagga aaaacggcaa aaacttgatc agctattaga tccgtttatc cgaaaagcga
     3901 taaaagatca aatcgcctct ctaagttcca atcatccttt agtaattgta gatatcccat
     3961 tgctctacga aggtcattat gaccactata tggatgcagt agctgtcgtt tatactacgc
     4021 cagaaactca gcttcagcgt ttgatgaaac gtaatcaact gacgctagaa caagcgcagc
     4081 agagaattag tagtcaactt ccaatcgaag aaaaaaagca gcgtgcggat attttgttcg
     4141 acaacaatgg aacaaaagaa aaacttgtgg gacaaatcga aaattggctt tttgctaatc
     4201 gctttctttg agtatctgtg tcaattttgc tatcctgagt atatagaaag tgagggatag
     4261 cgatgggatc attaaaaaat caaaaggata aagtcgtagg taacgcaaaa gaaaaaatcg
     4321 gcgagatgct caataaagaa aatctaatcg atgaaggtcg tacgcaatca gaaacagcag
     4381 aagaaaaaga aaagcagtac caagcaagaa tcgatgaaat agaagaccag caggcagaag
     4441 aagaagcgaa gcggcaagat ttgactcaag aacgaaaaaa tccgacacct gatcgtagta
     4501 aaacaccgct cccagaaagt gaccacattc ccgatgagca aaaaatcgag gaacagcgaa
     4561 tgaaacacta tacaggaatc gaagagaagt tataaaatag taaaaaatcg agatgtgact
     4621 tgaagcagat gagaaggtgt tcatctgctt caagtttttt gtgctagatg actaaatcag
     4681 cttttgtgta agttatttct cagtaatcaa ggtgatcagt attggaaaat tgaaatttag
     4741 taaggacagg ggacacaatt acgcatgaaa gtagaaaaaa ttgttcttat atccgtgttt
     4801 tttgggtagg acagtcgcgt ttgtgatttg tttcgtgtta taatacaact agttgtaaag
     4861 aaagacacat cctttatata aaatgcgatg caagaggtga gacaaaatga gatgtcccag
     4921 atgtcaacat aataattcac gagtaatcga cagccgtcaa gcagatgatg gacgtgcgat
     4981 ccgtcgccgt cgcgaatgtg agaattgttc gttccgattc actacatttg aacgaattga
     5041 agctgctccg ttattagtaa tcaagaaaaa cggggaacgc gaagagttca atcgcgataa
     5101 aattttaaga ggactcatcc gttcagcaga aaaaagacct gtagctatgg aacaaatgga
     5161 acaaatcgtc aaccatgtcg aaaaccgcgt acgcagtctc ggagaaaacg aagtatcgac
     5221 gaatctaatt ggtgaatatg tgatggaaga tttagttgat ttagacgaaa tcgcttatat
     5281 tcgttttgca agtgtttatc gccagttcaa agatatgtct gttttcttaa aagagttgca
     5341 agatatcgtg gataaagcaa aagaaaataa taaagagaat aataaagaaa acaattaaga
     5401 aacaaaaagg aggcatttcc ttggaaaatg cttggaaaga gcttcaacca aaaaatattt
     5461 accaaggaag aaaagcggct ccgctgagag aagacagcaa gaacgccttg ctgtatcttt
     5521 atcagccact aatcggcgga gaagccttga gtatctattt caccctgtta tcagaaatct
     5581 ctcaagaatc tggcaaaggt cctgaaggga tgcatgcaga tctgcttagt agtctaggct
     5641 gcggtatccc gcaattttat gatgcaagaa aaaagctaga aggaatcggc ttactggatg
     5701 tttattttaa agaagatagt gatctgggac ctcgttttat ttatgagctg ttagaaccac
     5761 tcattcctaa agcatttttc aaagactctg ttctggcttt tcttcttctg gaaaaagttg
     5821 gtgaacgacg attccagaag cttacccaat tctttgaacc taaaaggcat acagcagagg
     5881 gatatcaaaa aatcacaaaa aaatttttag atgtttatcg cttcaatgaa gcagtgtacg
     5941 ctgctaatca agaaaacatc gaatcgctga ataaatcttt tgaagaatct gaatcagtag
     6001 cattacttga agcagattcg acattcgatt ggcagttttt gaatgattgg ctggcaaaaa
     6061 aacatattgc aagtcttgat gatccaatca tcaaacaagt cgacatgtat catcagcttt
     6121 acggtttcga tgaattgaca cttggcgaac tgatcagtca atcttatgac tttagtgaac
     6181 aaaaagtatc tgaaaaagaa ctgcaaaaag ccattttggc aatgcagaga cgtccggtga
     6241 agcaaacaaa agaaacagtt gcggtttctg aaaacagtaa tgaaccctcg tcaaacgaac
     6301 atttgacaaa cgcagcgctt cagctgataa aagaagcaca gagcattcca ccgatgaaat
     6361 atctggaagc aatcaagtca gaaaaaggcg gatatgtttc taaacaagaa aactggttac
     6421 tgcaagatct tgtttctcaa tcaggtcttt ccagtagcgt gatcaatgtt ttgctgaatt
     6481 atgtactagt gatcaagaat aatgctagct taaatgccag ttatgtaaat acagtggcaa
     6541 atgaatgggc acaaaagaaa atcgccacag cagaagaagc gatccagcac attcggcaaa
     6601 taagtaatca agcaaaacaa tcaaaacaaa agaagcaaac aaattatcaa actggcaagc
     6661 gaaatgttcg acgggaaaag cttcctgatt gggtcaacca gccaaaagat gaaaaacaga
     6721 tcagcagcga gaaaaaagca gagattgacc gccgttttaa agaatacctg gcaaaaaagg
     6781 agggtgatat ctagtggaag acgtaggcaa agaattgaag aggatcatca gcaaacgcaa
     6841 ttatcaagaa aagtatgctg aaatgatcgc tgaagtctta aatgatgcgg atgttcaagc
     6901 ttttctaagc gcacaccaag aagaattgac acaagcggat attgaaaaaa gttacgctaa
     6961 attgtatgaa ttcgtgcaag aaaagcgtaa atatcaaaaa aacgatccaa cgatgatcgc
     7021 tcccggctat gagccgaagc tgacgttgaa ttttcattat gtggatgtca cttatgtacc
     7081 aacggaagca ttgttagcac ggcaaagaca agaagaaatc agaagccgag taaaagcaat
     7141 ggatatgcca aaagatattc aatcggcttc ttttgaaaac tttgaacgta cagatggacg
     7201 aggatatgcg agcttagaag ccctagattt tgtagagaaa tatactgctg atccaaaagg
     7261 gtatcataaa ggactgtatc tagtaggaag ttttggtatt ggaaaaacat atttattagg
     7321 agcaacagcg agagaattag cgattgctgg attcaccacg acgctagtcc attttccgac
     7381 atttgccgtt gagatgaaac aggcaatcgg aaaagatcaa gtggcagaaa agttagatgc
     7441 agtgaaacgt tcacctattt tgatgatcga tgacattggt gcagatgcga tgagtagttg
     7501 gatacgagac gaagtattcg gtgttatcct tcaatatcgg atgcaggaac agttaccaac
     7561 cttcttctca tcgaatttta caatggatga attggaacag catttgtccg tcactcaacg
     7621 tggagatgaa gagccgctaa aagcgaaacg gataatggaa cgcatacgtt atttgacaaa
     7681 agaaatcgaa atgactggtc gcaaccgacg caatggttga acagttgaca agtccaactg
     7741 acatttgacc tagtagtaaa agcaggtcag cgaatcatta tatggacaga aatgaccgga
     7801 gacaatcacc gcaacaagta atgcagaaac ttaaacggag gattttttat gagggctatt
     7861 ttttctagta tatctgtagt tggtctattt tttcttttag caggctgcgg tagcgcagat
     7921 agcaacacag gttctgttga ttcgattgat gcgggtcaaa cagtaaaaac aagcagtacg
     7981 aaaacggaga gacaaagcat atttgtcgga acgattgaaa gtatcactca agtagaagca
     8041 gaagaagaaa cgcttcagtt gaaactcgtc gatgtcatgg ccaaagaaga tccagcgaat
     8101 atgggaacgt cattcagcaa tgacggagtg atcttaaatg ctactgctga aaaaataagc
     8161 gtggacataa atgaattgaa aatcggtgac aaagtccaat ttacattagt tgagatgccg
     8221 atcatgacga tgtctatccc accgcaagtt cctggagatt cgattattga tgtttcattg
     8281 aacaaataac caataacatt ttttcaaaaa taagaaaatg ttgttttata gaacgaaaag
     8341 gtatattttt taaggagata tgggaatttt tttattccta gtctccttat tttatttgga
     8401 aaatgctata attatctaga aggagtgaag ctatgaacca tttagattta tctcagcgct
     8461 tagccgcagt gggtgacttt gtccctgaat ccgctcgttt agcagacatt ggatcagatc
     8521 acgcttactt acccgttgca ttgatattaa gaggaaaaat cgaatatgca gttgctggtg
     8581 aagtcgttca aggtccttat gaatcagcac gtaagcaagt caaaaaagat ggattagaag
     8641 accgtatcac cgtacgcctt gcaaatggtc tagatgctat ccagccacaa gatggtatta
     8701 cagctgtcac gattgcgggt atgggaggtt cactgatccg agatatccta gaagctggca
     8761 agttgaatca acgcttaagc ggacaagaac gtttgatcct tcagccaaac atcggagagc
     8821 gtacattacg atcttggctg caagaaaatc agtataaaat ccaatcagaa aaaatacttg
     8881 aagaaaataa aaaaatatat gaaatcattg tagccgaaaa agaacaaaaa ccagttactt
     8941 acaatgaaat ggagctgatg ttcgggccat ttctgcttga agagaaaagt gagatcttca
     9001 aatcaaaatg gacaagagag cttcaacaaa gaaaagctat ccgtgaacag ctcaaacaag
     9061 cagctaatca agaggcacgt caaaaacaaa tcgagcagga aatccaaatg attgaggagg
     9121 ttttaaagcc atgacaatca gcggacgcac tttcatccaa aagtttgaaa gctattgtcc
     9181 tcagtggcta gctgaagaag gagacccagt cggactgcat atcggaacat tggataagcc
     9241 aatccaacga gtaatgatga cattagacgt tcgtccagaa gtggtcgaag aagcaatcaa
     9301 gaaaaaaatc gatttgctga ttgctaaaca tcctccaatc tttcgtcctg taaaacggct
     9361 ggttactgat cagccacaag aaaaaatgta tgcagatttg ttgaaacatg atattgccgt
     9421 ctatgcagca cacacgaaca tggatattat ttgggatgga ctgaatgact ggttttgtga
     9481 acttctcggt attgaagtgg agagttattt agtaaagact catgagattc attataaaaa
     9541 actggccgtt tatgtacctg tggatcatgc acagaaaatg cgtgaagtat tagctgcagc
     9601 tggtgctggt acacaaggtg attataccgg tacaagtttt acctcgattg gacacggtcg
     9661 atttactccg gaagctggtg cacaaccagc gattgggaaa gtagggaaga cagaacaagt
     9721 tcaagaggca aaagttgagg ttattttgcc ggaaactatc gaaaaacaag tgattcaagc
     9781 catgcggtca gctcatcctt atgaggaacc tgcttatgat cttttcgcta tagatgaacc
     9841 agtcgaaatg tttgggttag gacgagtagg tgaattgccg caagaaattt ctatagaggc
     9901 atttgtagaa caagtaaaag aagcgttcca actagatgga ctgagaatag tccagccaaa
     9961 aaatgcaaaa tcctctgtga agcgcattgc gatttgcggt ggaagcggtg aaaaatttta
    10021 tccgcaagca atcgcccaaa gagcagatgt ctatatcacg ggagatatct attatcatac
    10081 ggctcatgat atgcaaagcg ctgggttgat tgcaattgat cccggtcatt atattgaatc
    10141 gttatgcaag caacgattta tcgagaaatt tgaatcttgg aaacaagaag aaaattggga
    10201 tattaacttt ttcgtttctg aaacaaatac aaacccattt caatttaatt aaaaggagag
    10261 aatactatgt acgaaaatct tttgccacgt tttttacgat atgtcaaaac agaaacacgc
    10321 tccgatgcaa caagcaaaac gactccatcg actcagacac aagttgcttt tgcgcaagaa
    10381 ttgaaaaaag aattagaaga gttaggctta gctgatgttt tctataatga agataacgga
    10441 ttcgttatgg gaacattacc aagcaatgtt gatcatgaag tgcgttccat tggatttatt
    10501 tcccacatgg atacagcaga ttttaatgca gtaggagtca atccacaaat cattgaaaac
    10561 tatgatggcg agtctacgat cgtactagat gcggaagaac gctatacatt gaatacaaaa
    10621 gattttccaa atttgaaaaa ttatgaaggc cacacattga tcacaacaga tggcagtaca
    10681 ttactgggag cagatgacaa atcagggatt gcggaaatca tgactgcaat ggagatcttg
    10741 atcaataatc cttctatccc tcacggtacc atcaaagtgg cttttggtcc tgatgaagaa
    10801 atcggtgtcg gagcggataa attcgatgtt gagcaattcg atgtagattt tgcttataca
    10861 gtcgatggtg gtcctttagg tgagttgcag tatgaaacgt ttagtgctgc tcaagcaaat
    10921 attacgatcc aagggaaaaa tgtacatcca gggacggcaa aagatacaat gatcaatgct
    10981 ctacagttag caatcgattt tcataatcag cttccagccg atgaagttcc agaaaaaacg
    11041 gaaggatacg aaggtttctt ccatttgatg gcattggatg ggaatcctga agaagcttct
    11101 atgagttata tcattcgcga tcatgatcgt gaaaaattcg aagcacgcaa agcacaaatc
    11161 acacatatcc aagaagagtt gaatcagcga tttgatcaag aacgaatcaa agtagatatg
    11221 tatgatcaat attacaacat gaaagaaatc attgaaaaag atatgagcat cgtggaatta
    11281 gcgaaacaag caatgatcga gcttggtatc tctccagtga tcgaaccagt tcgtggagga
    11341 acagatggtt caaaaatctc ttacatgggt attccgacgc caaatctatt tgccggagga
    11401 gaaaatatgc acggacgatt cgaatttgtt tctttacaag taatggaaaa agcaacagac
    11461 gtgattgtaa aaattgcaga attgaacaca aagagttaaa atcgaaaaag aacagcttat
    11521 gacaaggcgt tcgtcccaat gatggaatgt catctgtcta aggctaaagg gcaggacaat
    11581 ggaattggtt gtcccgccct cttttttgaa gagtaaatag gaggtaaaga atggaaaagc
    11641 tgctagtttt ttataacaaa agttcaggga aagatgaggg tgagaaatta gcacgctggt
    11701 ttcaagatta tgtaaaagaa aagcagccag aattatatgt agaactagct gaaactggcc
    11761 catcgattga tgatgcggaa ttagtcaaga aagcagaagc catagaagct gatactttag
    11821 taattatagg tggagacggc acgattcatc atatcgtcca agcgttccaa aaaacattat
    11881 ctcaatacca tgtcggatta cttccaggtg gaactgtcaa caaccttgca cgtgttcttg
    11941 agattcccct caaaaaagaa gaagctgctg acgtgatcct ggaaggcaat gtacggaaaa
    12001 tagactatgg tcaagtcaat gatgaagtca ttatctctac actgacaatc ggaatcttag
    12061 cagatacagc agtttggatc agtcaagaag aaaaacagcg ttacggctcg tggatcttta
    12121 tcaagaagtt ttttaagtta ttgctgaaga agaaaaaata tcatttggat ttagaaacag
    12181 acgaggagca ttggaaagga aaatcccaac tgttgacagt cacgatgtct aactccgtgg
    12241 gcggttttac aaattttgac gactcagcga ccctagatga tggaaaattt catgtaacga
    12301 tcgtaccttc cttagatatt ttccgattta ttttttattt gcctagaatc atgagaggca
    12361 aattctatac gatccccggc attacttatt tcacagccaa acgcatgaaa atccaagcgc
    12421 aggaaaaatc tgtgggcact cgaacggatg gagacacgac agatgatctt ccagttgaat
    12481 tatctgttgt atcaaaagga ttaacagttt attcaccaaa gaaagaagaa aaagcttaga
    12541 aatagtagtt aacagaaagt tggaagaaga gtaatggatg tattgttaca caatcctttg
    12601 tggttttacc ttttcattgt gaatttttac ttattttgtc tgatggggta tgataaacat
    12661 caggctagaa ctagcggctg gcgtgttcct gagaataatt tgatatttgt cagcctgtta
    12721 ggcggtggtt tgggaggatt gatctctcga aaagttttcc atcataaaac tcggaaaaag
    12781 aagtttacta taggattcgc tgcaggtgta cttgttgatt tgattctcat ttattttttc
    12841 cattaaggag caggagatgt cgtctaaagg aacgaaaact aaaatcattc tgaatctgct
    12901 tttgctgatt gtgatttttg ccattattta ttatttaatc aatcaatcat ttgcagatat
    12961 ctttgccgaa ttgctgtcta ccagtgttgt tgttttggca gcggctgtta tattagggac
    13021 agtttaccaa atcgctgaag ggcgctcaat caaagaaatt gctgaacctt tcgcgccgaa
    13081 ttttacaacg aaagatgggt tcttgacctc tagctatatt gcgttttacc gaatcgtttc
    13141 gtttggtaca ggaacgctga tctcggagat ttatttttat aacaaaaaag gaatgaagct
    13201 atcccaggga gcaggggtga cagcactcca tatgattatg tacaaactgg cagtgatcac
    13261 ttatgcatta gctggcttgg tcatccaatt ctctcttttt tattctaatg gtccatcaat
    13321 gatctggttt atcatcgctg gtatcctttt gacgatcgtg attgttgcct ttttattggc
    13381 gatgtccgtc agcttgaatg ttcaaatatt ttttgtcagc ttatcgaata agttatttaa
    13441 aagtgagcgt ttgcggaaat gggtggatac atgcaatcag cagatctact ctttaaggga
    13501 aacggtccaa accattatcc aagacaggac tgcgctattg cggatttatc tatggaatat
    13561 gttaaaactt gtattttggt atgtacttcc ttatttgatt ctgattgaaa accatcctaa
    13621 aatagatttt ctactgacca tttcgtttat cagctttgca gtagtgcttt caggggttat
    13681 tcctacgcct gctggtattg gttcctttga atttgtctat ttgttattat tcagaccagt
    13741 tgttggaaca gttgacgcag tttcttcgct tttactgtat cgtttcagtt cctttatctt
    13801 gccatttctc tatggatttg tgtatgtgat tgctgaacgg cgaagagtga tcaaagaaga
    13861 aataatagaa gtaaaaaaag agaataaaga aaatcaaggg taggtctttt ataagatcta
    13921 ccctttttat caaatcaaag aaaaacgtta tggataagat aattttacgt ttgtttggct
    13981 ctttttttat atacatacaa tcaattttgt ttttttaaga tttatataag tcgagaaaaa
    14041 aagtcaaaaa actatgtgta tattttgacc aaaattgacc aaagcgatat aatagttttt
    14101 gtaaagatca aagaaataag tgatcttacc tttaattagg agggtatacc tgtatgaata
    14161 ttgagaaaat gactacgacg cttcaagaag cgattgctga agctcaacag attgcggtca
    14221 ctcgaaaaca tcaagatatc gacattgccc atgtatggaa aatcttttta caacctaatc
    14281 attttggtcg aaatttctat acggatgccg gattggatgt cgaatctttt gaacatgaaa
    14341 ttgatcgtct actggatgag tatccagttg tttctggtgg aaacgtccaa tatggacaaa
    14401 acctcagcca gaatttcttt cgattattga atgaagcaga ccaaatacgt gaaagcttcg
    14461 gcgacgagtt tctttcaacg gaagtcgtta ttttagcgtt gatgaaatta aaaaattatc
    14521 cgttaactgt ttatttgaat aaaaacggat tatctgaaaa agaattgcgt aaaaacatcg
    14581 aagagatgag aggaggagaa agagtgacct cacaaaacca agaagaacaa tacaaagcat
    14641 tagaaaaata cggcgttgat ttagtacagc aagtcaaaag cggaaaaatg gatccaatca
    14701 tcggtcgtga tgaagaaatc cgtgatgtca tccgtattct ttcacgaaaa acaaaaaata
    14761 atcctgttct gataggagaa cccggtgtag ggaaaacggc aatcgttgaa gggcttgctc
    14821 aacgtatcgt acgcaaagat gtgcctgaaa atttaaaaga taaaacgatt ttttcattag
    14881 atatgggtgc attgatcgct ggagcaaaat tccgtggtga atttgaagaa agactaaaag
    14941 cagtcttgaa agaagtcaaa aaaagtgatg gacgtatcct cttgttcatc gatgaaatcc
    15001 acaatatcgt aggggcagga aaaacagaag gaagtatgga tgccggaaat ctgttgaaac
    15061 caatgcttgc tcgcggtgaa ttacatttga tcggtgccac aactttagat gagtaccgtc
    15121 aatatatgga aaaagacaaa gcgttggaac gtcgctttca aaaggtatta gtcaaagaac
    15181 caacagtgga agatacgatc agtattctgc gtggattaaa agaacgtttt gagatccacc
    15241 atggtgttaa catccatgac aacgcattag tagcagcagc tactttatct gaccgttata
    15301 ttactgatcg gttcttaccg gacaaagcaa tcgatttgat cgatgaagcc agtgcaacga
    15361 ttcgggtaga aatgaactct atgccaacgg aattagatca agttactaga cgtttgatgc
    15421 agttggaaat cgaagaagct gcactgaaaa aagaatcaga tgatgcaagt aaaaaacgct
    15481 tgaaaaattt gcaagaagaa ttagctgaac ttcgtgaaga agcaaatgcg atgaagatgc
    15541 agtgggaaac agaaaaagaa gaagtcaatt ctgtttcagc aaaacgtgct gaaatcgata
    15601 aagcaaaaca cgaattagaa gatgcagaaa ataactatga tttagaacgc gctgctgtat
    15661 tgcgtcatgg tacgatacct caattagaaa aagaattgaa ggaattagaa gctaaagcaa
    15721 aagacagcga gattaagatg gttcaagagt ccgttaccga aaatgagatt gctcaagtcg
    15781 ttggcagatt aacgggtatc cccgtaacga aacttgtaga aggtgaacga gaaaaactaa
    15841 ttaaattgaa tgaaacactt cataaacgag tgattggtca agatgaagca gttgatgcgg
    15901 tcagtgacgc ggtaattcgt tcacgggctg gattgcaaga cccaaatcgt cctctaggtt
    15961 ctttcctttt cctaggacca actggtgtcg gaaaaaccga attagcgaaa gcgttagctg
    16021 agaacttgtt tgactcagaa gatcatatgg tacggatcga tatgagtgaa tatatggaaa
    16081 aacatgctgt gtctcgtcta gtcggagctc ctccaggcta tgtaggttac gaagaaggtg
    16141 gtcaattaac ggaagctgtc agacgaaatc cttatacaat cgtcttgttg gatgaaatcg
    16201 aaaaagcgca tcccgatgtc ttcaatatct tgttacaagt cctagatgac ggacgcttga
    16261 ctgattcaaa agggcgagta gtagatttca aaaatacggt tctgatcatg accagcaata
    16321 tcggttcaca gttactgctg gaaggcgtga ctgctgatgg aacgattcca gaagcagtgg
    16381 ctgaacaagt caacacgcta ttacgtggaa acttcaaacc agaattcttg aaccgaatcg
    16441 acgacacgat tttatttaca ccattgagcc tagacaacgt gaaaggaatc gttgacaaaa
    16501 tggtggcgca attggcacaa cgtctggaac atcaagaaat cctgctgaca atcagtgatg
    16561 aagccaaaac gtggattgct gaaaatgctt atgaaccagc atatggtgca agaccattga
    16621 aacgatttat cactaaggaa gtagaaacac cactagctaa agaaatcgtt gcaggacatg
    16681 ttatgcctaa atcaaaagta acgattactt tacttgatgg acaacttcat ttcaaaacag
    16741 aagagctaga agaaatcgtc taatcttcca ttgagactat aaaagaaaaa aggggtctga
    16801 cacaactagt gtcagacctc cttttttcaa ataaacagtg ttccagaagt agtttcttcg
    16861 aaaaataagc cgaaatttct tgaagctgcc cgcttctgtc acaacctctt ttattaggta
    16921 tttttcactt tttctaattt ataactttca aagtaacgct catctttttt actttgagac
    16981 catgcaaaca atcccattaa ggcagcacct atcgtatttg tcagcaagtc gcccatcgta
    17041 tccatcaatg ctgctcgtcc gacaaacagt gtgccatcag aagcagcaaa acgttgaaga
    17101 ttcatgccta agaactggtc gcataagaat tcccagaact cccaaaacac gccgcaaagt
    17161 ccagcaaacg caaagccgaa taagaggaac aaccaaggac tggtttttga tatctcagca
    17221 tctttcatca agtaaccaat caatccatag ccaagagctg tcaggaccat tggactcacc
    17281 gcatgtaaga ttttgtccca aaaagagatg atactgatca aatgcatccc tgtgcctaaa
    17341 aagacagaga tgaacaagaa aaaccagtaa aacaaaacga ttgctgctgg tattttaagt
    17401 ttgaaaactt tcaaaatgac ttgtggcatg aaaatcaatg caatccctaa taaagattcc
    17461 acgataaatg tgattccttg agaagtcgaa tatttttgtg tgataaattc ataaaaatta
    17521 tagcagacaa cgaatacacc aaaagcaagt aaagctattt gcaccttttt gatttcttta
    17581 atcactgaaa gtccctccat ctaaaaatct ttttacttct gaaagcaact caatttctga
    17641 agaaaccact tttccgttca atttgatcaa acctattgta tataagttca ggtagtgaaa
    17701 ttgattttca gctgtttctt gtagtgcagc gatttttttc gggtgggttc cgccttgctg
    17761 tcgagtatca gtatataagc cgatcactgg gatgcctttc gcataagcca caccgatttc
    17821 cgatgctacc ccggcatcga tcgtcagccc atctagtaaa gcaatcatta aatcactagc
    17881 caacactttt tctgtgtcag caagagcaat cattcgactg tctgcataag cagttttgtc
    17941 attgattgct tcattttctt gaggaagata gatcgaaagc tcagaagaga gctctctgat
    18001 ttctttaact aactgttggt tatataacaa atcactttta gcaaacattg gtgcggcaaa
    18061 ataaattttc atctaaacat catccttttt atcaaaaata atcgctaaag taagcttcca
    18121 tgtttggaat gatacgctct agtgacttca gtgtttcttc atctgtttct aaccgttcga
    18181 ttcttgccaa ataagaagca cggcgataat tcatcatcaa gcaagtcaat gtttggatat
    18241 ccagtcgaac ggattttcct ataggttcat ttaagacacg gacttgtccc tgttcatccc
    18301 atgttaaagc gaagacacca ttattccaag gagcgacagg atcttcaatg atgaaatgaa
    18361 aagcatcagc tgttccaaca aacggaaacc gttgaaggaa ttcttttaca tcaacgatcc
    18421 gagccataaa atatggttcg atctgctctt tgatctgact atcttcaatc aaaaatgcta
    18481 aaggctcatt tttataaata tctccatgta cccagtagat catggaaaaa tgtgcggaaa
    18541 taaagttcca taagccattt cgagcttctt gatcaagata gaacatttct tttacgtgaa
    18601 atatttcttc agctacccag tagaacagaa cacctcttgg ttcatggttg ctatcgtagt
    18661 agacagcggc tgtgcgctca tcttcatttt cgaaacgcca atactcttcc caatgaaaat
    18721 cactgcggaa taaagcacca tggttttgtt gggcaaattt gtcataaacg gaaaatacgt
    18781 ctggatgatc aactggtaat cgctcaacca taccacttac tgggatttgt ttaggtagct
    18841 gtgtatctct gattttaaag gaaagtttat cagacatgat ttcccatcct ttacgccgat
    18901 agtaggggat actgtaagga tacaaataag agatccattg cttattcttg cgcatattat
    18961 ccaatgcaac gataattaga tcttgcatca atccatggtt agcatattct ggataagttc
    19021 caactccagt cacaccaccc attttataac gtgcaccgtg aatattgact tcacaaggat
    19081 agatagcgat ttgagagatc agtttatttt cgtggaacca tccgaacact ttcgatactt
    19141 ccaaaatcgg ttttttggaa cggataaaag ctcgtttatt ctcaaacccg ctttcctcga
    19201 tgtctgcttc agtcacttgg aacacatagg aaagcaattc attgaattga tcgatatgtt
    19261 cttgagtgac cggttttaat tcaagatttt ctctaaaatc tttttgatcc attttttcat
    19321 ctccatttat tagcttgata acagtatagc aaaattctac aaaaaaaact tctatagctg
    19381 actaattaaa aactctttaa attactgctt ttcgttggta taatggaaag tgctgattat
    19441 atgaaaagag gagacgctat ggatataaag aaaatgaaag aacgccaaga gcagattcgt
    19501 aatttttcga tcatcgctca tatcgaccat gggaaatcta cattggccga tcgtatttta
    19561 gaaatgacgc acactgtgac gtctcgcgaa atgcaagatc agctgttaga ctcgatggat
    19621 ttggaacgtg aacgtggaat cacgattaaa ttaaatgctg tagaactcaa ttacacagcg
    19681 aaaaatggag aaacatatat ctttcacttg attgacaccc cagggcacgt cgacttcacc
    19741 tatgaagtct ctcgaagcct tgctgcctgt gagggtgccg ttttagtggt cgatgcagca
    19801 caaggaatcg aagcacaaac attggccaat gtttatttag cgttagacaa tgatttggag
    19861 atcctgccag tcatcaataa aatcgaccta cccgcagcag atcccgaacg tgtccgtcaa
    19921 gaaatcgaag acgtaatcgg aatcgatgct agtgaagctg tattagccag tgccaaatca
    19981 gggattggga ttgaagatat tctcgagcag atcgtggaat atgttcctgc accaagtgga
    20041 gacttagatg caccgctaaa agctttgatc tttgactcta tttatgatag ttaccgcggt
    20101 gttgttctaa atgtccggat cacggacggt atggtcaaac ccggagacaa aatcaaactg
    20161 atgagtaatg gcaaaacatt tgatgttaca gaggtcgggg tcttctcgcc aaaagcagtt
    20221 gctagagact ttttgatggt cggtgatgtc ggttatatca cagcaagtat caaaactgtc
    20281 caagatacac gagtagggga tacagtaacg ttagcagaca atcctgccgc tgaagcttta
    20341 ccaggttacc gaaaaatgaa tccgatggtt tattgtggtc tttatccaat tgatacttct
    20401 cgttacaacg atttgcggga agctttagaa aaactgcaat tgaatgatgc tgctttacaa
    20461 ttcgaaccag aaacctctca agcattaggt tttgggttcc gctgtggatt cctcggactt
    20521 cttcatatgg atgtcgtcca agaacgcttg gaacgggaat tcaatctaga attgatcacg
    20581 actgcaccat ccgttattta ccatgttaat aaaacggatg gtacgactgc tacagtagat
    20641 aacccggcag attttcctga acctgtaacg attcaggatg tagaagaacc attcgtcaaa
    20701 gcacagatca tggtcccaaa tgattttgtc ggtgcagtaa tggaactttc tcaacgaaaa
    20761 cggggagaat tcattacgat ggattattta gatgattacc gagtaaatgt cgtctataat
    20821 attccgcttt ctgaaattgt ctttgacttt ttcgacaaat tgaaatcaag cacgaaaggc
    20881 tatgcctcat tggattatga aatgtctggt tatcaaaaaa gcaagttagt gaagatggac
    20941 atcttgctga atggagaaaa agtcgatgca ttaagcttta tcgttcaccg agactttgcc
    21001 tatgaacgtg gaaaagcaat cgttgaaaaa ctgaaaaaac taatcccacg ccaacaattt
    21061 gaggtgccaa tccaagcagc tattggacaa aaaatcgttg cccgttcaga catcaaagcc
    21121 ttgcgtaaaa acgtcttggc taaatgttat ggtggagatg tttctcgtaa acgtaaactc
    21181 ttagagaaac aaaaagaagg gaagaagcgg atgaaacaaa tcggctccgt cgaagttccg
    21241 caagaagcct tcatggccgt tctgaaaatg gatgagcaag atagtgtgaa ataacttata
    21301 atcaatcgaa aaacctatta aataaacctt tttgagtaga tgcaacactg aggattatct
    21361 gtataaaaga ttttaaactg tcatttggtt atcacggcga tactaaaatt aacaaaattg
    21421 gaatattttt cttgaagatt tttagctgtc agtatatgat gaaagcatct cataggaggt
    21481 gcttttttca tggttgataa ttcaagtgga agggttctta aatctctaag aaaagagaaa
    21541 aaactatctc aaaaaaagtt agctgattta gcaggtattt cacaaagtac tttagttaaa
    21601 tatgaaaaag ggtcaagaaa aatccctaaa gacgtagata atactctaag caagattctg
    21661 aatatagaaa ctctaataaa agatgaggaa gataagattg agattctcat cggtaaacta
    21721 attgcataca gagatatgaa taagttactt aacaaagagt tagctgataa tataggaatt
    21781 agtgaagtat tactcagtta tgtgttaaat agaaagagaa atccgtcaaa agaaatgcaa
    21841 aaaaaaatcg atatttttct attaagtaat gaaaaagaaa ttttgaagga gattaatcgt
    21901 gatagtgaga tatttagttt atcaaaagac gataaaattg ttatgggaaa aagaatacga
    21961 gaagtccgaa agaaccggga agagacgctt gaaaaatttg gtaaaaactt taccatctat
    22021 acaggcaaaa atgttataag tagatgggaa aaaggtatta atattccaga tattgaaaaa
    22081 ctaatgaata ttgcctatct tggtaaggtc actgttcctt accttatgta cggagaagat
    22141 tataagaata tacttccaaa agatgaaaga gttagcgatt ttaaaaagat aaattctttt
    22201 agtatgggat taaggatgag gaaaattagg aaagattatt atctagaaag ggaggagttt
    22261 ggaaagcttt tttctccttc aatttcaaaa tggtccatag ataggtatga gaatggtaga
    22321 gatataccca atactaatcg aattatccaa tatgcttata taggaaatct ttctttagaa
    22381 tttttgattt acgggatata aaaatagttt acgtcgatac ttattaagtt atcgacgttt
    22441 tttattatag aaaaaattta taaaaggagg tttcttgata tgataagaaa caaaaagggt
    22501 ttgttgaaag ttgctttact ggcagctttt ttctaagtaa ctttttgaca agctcaatgg
    22561 caacacaagc ggaagaagta atacaagaac taactgttat gaataacagc tagataaaat
    22621 gggctatgta tgttatccaa caatttagta atcactagaa gcctagcgaa agttcgcttg
    22681 ataagttata gatgagtatt gatttgtagt gtttccaaat caaaagggta cagagccaca
    22741 tgaaatatgt tgtcctctgc agcgtcagga tgggaatcca taccatggag attcggagat
    22801 ctgtgaggga atacttacgt ggttaatatg tcagttgtgt tggacgtagt aatggtcata
    22861 gtgggtagat tgactgaatt cttatatgag aaaatgaaac caacaagaag atagggttat
    22921 ttatacagaa ttggggatct tttctggaaa agtaatcgga taatgcgaga ttcgtacggt
    22981 cttttttaaa tttctctaaa aaaaacggca tcgaatgggt tgggtgattg ataagcaatg
    23041 aatcatagac tgatatagaa taaaaaatga ttctatttat ctttttacat ctatcgtttt
    23101 ttcaagtagc cgcaaattat atcagcattc aacaattttt gtatacaagt tctagtaaaa
    23161 ggatctgaag aaaaagttgg ttgttgtgaa gataaaaaaa gaatcaggtt ttactgagtt
    23221 tttcttcaaa atttgtgtta ttaggttagg acaaagataa gaagaagtat gaagatttaa
    23281 aaaaggaatg aggaaaagat agtaatttcc gattattgga gtttttttat ttataaagaa
    23341 gaagcgaata agttgtgatt cctagatttc gcgcgtgatt aattaatgag caaaaaatgc
    23401 ggaaagtttt agtgtttaat acacgaggac aaagtttctt aggtattgaa ggcacacgtg
    23461 taacggttga tgggtggtgt gattgtttcg aggttggaaa acatgttgaa cttatggcat
    23521 ttactgagct aaaagataag aacaacgtgg aatttttcga gagtgatatt ggttgggacg
    23581 atcatgaaga agtttatgga aaatttattt tgcggaaggg aaattcttat acgaatgggg
    23641 aagcataagt agcgatttgt tagagatcat tgataggatt gaaattgtcg ggaatattta
    23701 tgagaatcta gagttatttg aggtgataga atgagtgatg gttttgaaca agtatcaata
    23761 attaaaaatc aagttcggga gattcttaga aaaaaaggct atttggtaga tagttatttt
    23821 gaaggagatt acgaaacatg ggtaggtgtc tatgctcgtc cagagaataa acctacctat
    23881 ttagatccta cgacgtcaga agatgcctat ttacaaaata ggtatcgagt agatggtttt
    23941 aagcaagact ttgcggagtg gtttgaatgg gaaattgaaa acggagaagt aaaagaagaa
    24001 taagagtact tgttgtatca aaataagcca gtagaacaac caaaccagta gctagtaatg
    24061 acaataatac tgaggaatcg gatcattatg gtatgattga tatttctctt ccagaagatc
    24121 ttaagccaga tcctgtaaca attggcaata gtgtcatgat ttttgattca ttagatgaag
    24181 ctatgtcata tggttggaaa gaaattaagg atgaaggtag taattggtat ggcatgagat
    24241 ttggtgatgg tgaagttgcc aaaaaagctg gtattccaat tgggaaatgg acagttgatt
    24301 tccacgaagc aggttctttt tttgttctgt aattatagtg ggagggtaaa atcattgatg
    24361 aaatgtgttc agataagagc gccagataaa aatagaagag acagttcacg tcgttatcac
    24421 ttttggtaac ggcgttttgt tttattatat aaaattctca ggaaaaggag gaattcttgt
    24481 gtatgcaaaa acgaaaatga attggttgaa agcactcgca ttggtggctt tttctcaatt
    24541 ctgtttcttg gtgggaactg ctcaagcaca tgcagaagaa acgacggcta tacaacctac
    24601 gatggaagcc attgcccaag tcgcttctga agtgacagaa gtccaaccag cagaagccgg
    24661 ggtcccagct ggtgaagcca ctgttgaaca ggcccctgtg acagaagcgc caacggtcaa
    24721 ttccaaaacg actctcgatc agacgattgg ggaaacacaa cacccaggga atagtcctgg
    24781 tacttcacaa ccagttgaag aaactgcctc aattacaacc cctgcttcag aaacgtccac
    24841 tctagaagga gaaaaagctg ccgaagcaac gccttctgtg cagccagagt cttctgagaa
    24901 acaagcggaa gaaaagaaac gaatagaaga aacagtggaa caaccagcgg ctactgctga
    24961 caaagaagga aaaaatgagg aaaacgttga aaaaacaatc tcttcaaccg cagctagtca
    25021 aaatcaacca acgaaaccaa acaaagaatc tacggctagt attgaaaatc aaaagaaaaa
    25081 agagcagaac attccaaaaa caggtacacc tacactgtta gtaacagtac caaccaatcg
    25141 ttcggttcgt gttcgccgtg caaccaatgc agctgtttta ggtggtcgga aaattgattg
    25201 gggcgaaaat attggtgtta cttctagtcc gaacaatcta aacttcactt taaattatat
    25261 ggttcgtttt gagattaatg gacaagtggc gttttgtatt gagcctggtg tctcagccgc
    25321 agaaggtcaa gtttatacag ctactgcttt gaaaaactat ttgaaaaatt caaatgtacg
    25381 gaaaaaaaca agcttgatct cttatttagg atatgtcaca aataaagaca aatccgatga
    25441 acaatacatt gcggcacaat ttatgatttg ggaagctttg ggcacaaaaa tcccaatcaa
    25501 aaatatccgt gttgattatg atcgtcgaaa agcagaaatt caacgtttga ttaataactt
    25561 tggtgaaaaa gcaactttta ataatcaaac acatacggta aaaattggcg aaacgttgaa
    25621 agtaaagaat aaaaatgatt ttttgaaata tgtgaaagac attcaaaaac caaaaggtgt
    25681 caatgtatct attcacggca atgaagtatg gattacagtc acaaaagagg caccggataa
    25741 agcaaaaatt caattgaata aaattaaagt tcaaggtgcg ccattagcct atcgtcgtcc
    25801 aggagctcaa acaattggtg tgttacatcc aacagaacct aatgcgtctg ttattaattt
    25861 acaaattcta aaacaaggaa acattcgtgt gtttaaagaa gatgcggaaa ctggtgtgaa
    25921 agcacaaggt gcagcttctt tagaaaatgc ggtctatggt ttgtatcaag cagatgggaa
    25981 aaagattaaa gaaattacct tgaaaaaaat taacggcaaa gtacaggcag aaattaaaga
    26041 tcttgatcct ggcaaatatg ttttaaaaga aatcaaagcg ccaaaaggtt atgtgctttc
    26101 tgatgaagca attgaaattg ttgttgaacc tggaaaaact gtcaatgcga ccgcaaaaga
    26161 tcaagtgatc aaaggaacca ttgatttagt gaaagttgct aataaagatt tagtcgattc
    26221 aacgaatcca gataacaaac caaaattagc tggcattgaa atcactttga cgtcaaaaac
    26281 aactggcaaa gtggtgaaaa ccgttgtgac agacaatgat ggttatgcta cttttggtaa
    26341 aaataccgtt gtctttgata cgtacattct ttctgaaacc aaaggaaaag aagggtataa
    26401 actatttgaa ccacttgaag tcacaatttc tgaaaatggg caaactttcc attatgtttt
    26461 agaagataaa gtaattgaac aacgtcttaa agtggtgaaa gtggatcaag aaacaggaaa
    26521 agtgattcca cttgctaata cagaattcaa gatttttgat actttagcca atcgttatgt
    26581 cacaatggag attccaaatg atacagaaac aacggatatc tttaaaacca atgaaaaagg
    26641 ctttttccta actaacggta cacttactta tggaaaagat cgctatcgtt tggaagaaat
    26701 caaagccccc gtaaattacg ttttgaatca aaacccatta gtgttctcga ttaccaatga
    26761 tacagaaccg atcaaagtga tccattttgc gaaccgtcaa gcacggaaaa gtgtaaaatt
    26821 attcaagtat gaagaatgga acaagaagaa aacgccgctc gcaggtgtga actttacctt
    26881 gtataacggt aaaaatcaag caattggtga atataccact gatcaaaacg gggaaatttt
    26941 agtgaaaggt ctagttgccg atgactatta cttcttggaa aatgccccat tagaaagttt
    27001 ccaaccaaat acagataaac aagcattcac ggttgcattc aaacaaaatg cgccagatga
    27061 tggggaaacg ttagtggttt ctgtacgtaa ttacttgatt ccaccaaaac tagaaacaca
    27121 agcgtcaaat aaagccgatg gtgaaaaagt ggttgatcca ttagaaacga tcgtcattaa
    27181 agacagtgtt tcttatacca acttgtttgt cggaaaagaa tacaccattt caggagtatt
    27241 gatgaacaag gcaacgaatg cgccaatttt acaagatggc aaagaaatca caagtacctt
    27301 ggcctttatt gcagacaaag tcaatggcac aaaagacctt gaatttgtgg ttaatgctag
    27361 tgttctgaaa ggtcaaacga tcgttgtttt tgaaaaactt ttccgtgatg gcattgaagt
    27421 tgcagcacat gcggatatca acgatgaagc ccaaagtgtg aaagtgaata atccaaccgt
    27481 tcataccacg gctgtctttg aagacggatt gacagtcgca gacccaacag gattgtttac
    27541 cttaaacgat accgttgaaa ttaccgagat tcttccagga aaagcatact tggtcaaagg
    27601 gatcttaatg gataaagcaa ccggtgaacc cttgcttgtg catggaaata cggtagaaac
    27661 gagtgttggc tttattgcca aagcagaaaa tgaaacggtg atcgtaccat ttacctttga
    27721 tttgacaggt ataaatggtc gtgaaatcgt cgtgtttgaa agtcttttta gaaatgatga
    27781 agaactagca aaccataaag acatcaacga caaagggcaa acggtacgag taactaaccc
    27841 aagtgtacgt acaacggcta cacataaacc cactggcttg aaagaggtta atccattaac
    27901 gaaagtcacc attacagacg ctgtgaaata ccacgatttg gtcgttggaa aaacatacac
    27961 agtcaacggt gttctaatgg acaaagtaac ggaaaaacca gtgctagtag atggaaaaga
    28021 agtcacaagt accttaacgt ttgtggcaga aacaacggat ggtacagtca atctagactt
    28081 tattctagac gcacgtgcgt tgcgaggcaa agaagtcgtg gtctttgaag atctgtttag
    28141 agaaggcgta ttattggctt ctcacgcaga tattaccgac aatgaccaaa ccatcaaaat
    28201 cagtaatcca accattacga cgaaagcgac gaataaaaat ggcggaaaag agatcttggc
    28261 cttgccaaat caaactgtgc ttgaatgggt caaagtagac ggtcttgcga ttggtcaaat
    28321 ctatcgctta attgttcaag gctacttaac accagacggc acaccatttg aaggcactca
    28381 tgcggaaaaa gtctttgcgg ccgataaaga aatgatggaa ttcttatttg aattcttagt
    28441 ggacggtcgc aatcttgcag gaaaaggctt gagttttgcg gaatggctcc aagcgaaaac
    28501 ggaagatgag aaagaaacgt ttgaagaagt ggcgaaacac aatgtagatc taacaaacaa
    28561 agatcaaacg gttcaaattg tggaagcgcc aaaaccaccg caaccagtga aaccagaaca
    28621 acgggttaaa ccagctactg ttgtaccaat gttgccaaaa acaggtgaac aaacaagtag
    28681 tatacttatg gttcttggat tagtagtatt agtcattgct ttaggtataa cactttataa
    28741 agtacgtaaa gccaaagatt agtcatgagg aacaagcggt taggattttt aaccgcttgt
    28801 ttgattgtaa tgtagaaata tttagaattt attttggttt ttaagaggag gaaaaacttt
    28861 atggcagaaa aagcattgcg tatgaagaaa gagtttttag gtcaagtatt tttattgggt
    28921 tcacgggaac ctaatccacc aaaggaaatt aatggtgtgt ctgggcgata tgttcaatta
    28981 caagcagagt gttcgcgaga agaacagcct gctttttttg tttatctcac agctgaagag
    29041 gataaaagat tacgattacc agaaaaagag gtgcgtattt catttgaaga agcttatttt
    29101 gtttttcgta aagaacgagt gaatagtcgt gaaacaaatg atatgggaca aacagagcgt
    29161 cgaacaactg gctatcaaga tgttggtgtc tttttaactg gtgtaaaaat tggggaggtt
    29221 ttgtaagatg ggattaaaat ttccaaataa ttatgacatt acagaagcat tagatgaaac
    29281 ttgtttagga gccgtacgct ttggcgcatt agaagagtct gatcgtgtat ttgataatga
    29341 aacaggcgat tatacgggag aaattcgatc acagcgagca caattagttt ttggaaatcc
    29401 agatgaacca aagattgcac gacgagttaa atttccagca gatgtagatt tgactggttt
    29461 taaacgacgt gatcctgtga aattaattga tccagaatat catgtggtat atattccagc
    29521 acaagatggc aatcgtgaat attcagattt agtgatttat gcgaaaggat tagagaaagt
    29581 agaagctcca acaaataatc ctaatatgaa acaatcacaa gaaaaaggtt tagatcaaaa
    29641 acaaccaagt gttgattcta aggaaaaaga gaaaaaataa ggtttttttg aaagaatagg
    29701 aaggagtctt cctattcttt ttatctaaga tagaaggaag gggagatttt gaattcagat
    29761 aaaaccaatc aaatactttg agagattgtg cttgttatca aaatatccaa caattgattg
    29821 agtattttgg agacaatcca acgatttttg tacaaacaaa tgcatatatt tgtgcggtta
    29881 ttgggcagtg tatgttttta cgtgatcttg ttcgtttttc attaccgtac cagtggaaac
    29941 aagatcatca ggaagtgatt tggattcgta tagtacgaat atgtaatatg tgtgcacacc
    30001 cttataaaaa tcgattaaat ctttttcgat tatgtttttt tactaaatgt gatattcctg
    30061 tattagttgc tttttgtcaa aagtttttag cacaggaaaa gtaaaaacag aaaggaggaa
    30121 tgtgacaatt gaaagctcat tttttcaagt atcgtggtcg gcgaattcgc tatttcaatc
    30181 gttatttatg gttttgctat tatggtttct ttttgtttcc atttttgatt ctgttaggtt
    30241 ggtttagttg gaaagtattt tatccacgtt taagtacgtt ttcgaattgg caaatgtatc
    30301 ttattcctgg aataagtatt gtgttgtatt tgttgctggt tagtgggtta attttagggt
    30361 tgatgcattg gtcgcgacta aaagaaggat attttgcctc tgtttattgg cgtcagttac
    30421 ttgttcgaat gttaattgac aatcggtttg tttatacaaa gaaacaagtt agtgagaaac
    30481 aaacaaggga aaaaatgaga ttaccaaaag tttattttta tcaaaagaaa caagaattag
    30541 tggtttcttt tcctttagat ggtgggcgtt ttcacgaacg atttttaaaa atgggtcatt
    30601 tgttatctga agtatttttg cgtgatttaa ttcgtgaaga acgagaaaaa gcccgaatca
    30661 attatgtatt cctttcagat tctggtcgga ttcctattga tcaatgtata gcagaaaatg
    30721 gacgtattca tttaataaaa actttggatt gggtatatga tcaatcacca aatatgttga
    30781 ttgctggtgc aatcggtggt gggaaaacct atttattgta ttctttaatt caagcttgtt
    30841 taagtgttgg gacagtagat atttgcgatg gaaaagcggc agatttagca gcattaggtg
    30901 acgtcgatgt attcaaagga catgtgtttt acaaaataaa tgaagaaatg attatttgtt
    30961 tacgaaatgc attaaaagaa atgaatcaac ggtatgccta tatgaaaacc atgaaagatc
    31021 ctaaatatga accagggaaa aattatgctt attatgggtt accaccgcat tttatctttt
    31081 ttgatgaatg ggcagctttt tatggttcgt tggatatgga agcgagaaat agagtcgata
    31141 aattgattca gccattagta ttaaagggtc gtcaagcagg gatgtacatt attttagcaa
    31201 tgcaacgacc agatgcagaa tattttccaa gtggtgttcg ggataattta acattccgta
    31261 tttctgttgg tcgattgtct cccattggtt atttaatgac ttttggagaa gattttaaaa
    31321 ataagccatt ttacaacaaa gatcaacctg gtcgtggata tgcttttcca ggatttgatg
    31381 tacctgtaga gttttttgcg ccatttgttt acaaagatta tcactttatc aaagaatttg
    31441 agaaatttac tgaaatgatt gtctatgatt ttacagtaaa agaggatagt caagatcaaa
    31501 cgatgggaga tgataatcag ttgttacaag aacaactaga gcgccaaatg tatttagcaa
    31561 aagatccaaa aggaacaagt ttttctgatg cgcatatttt gtaatcaact aaggaggtga
    31621 agttaaatgg cagagagttg ttattataga gtagctacag caatacgaat gattaatcct
    31681 tctctttctt ccagaacgtt ttatgattgg ttgaatcgta ttgaacaagt aactgattac
    31741 cgctttttgc gcaaagaacg tgtttttaca ggaaaagtta ttaatcaagt attgctaacg
    31801 aaaaaagata tagaacgatt gactcgattg tatcattatc gagtagattt agaagaggat
    31861 ttaactttgt caatttatcg cgttttttct cctgaaaaat atagtgaaat aacaaaatta
    31921 gatcatttaa ttttgtagtt attatctatc gggagggctc tagacgtagt ctggtagccg
    31981 cactctgcct ggcggtgcgc attaaggctg cgaagcatgg gcctataatg cgcactcgct
    32041 agcagcgtgt gcagctcaca aagaggtcgt gcgcttgcgc acggcctctt tgtgtaaagc
    32101 cccccgatat ataatatggg gggcagaaat aacataacac atagaaaaaa gttttataaa
    32161 cccttttgta tcaagtgatt atctaaggtc agcagttgta ctacttttcc caaaaaacga
    32221 gggggactag ttgatatatc ggtgtttcta aagggttaaa aaaaatcgat tttgttattt
    32281 tataaaagga gcgagaaaat gacaccgatt gagctaaaaa gctacagaaa gaagttaagt
    32341 gcaattcata atataagtgg agataaaaag ccgctatcac aagcaagatt tgcgcaattg
    32401 atgggctgct caaaagatta cattagaaag atggaacaag ggaaaagaaa aatcccagag
    32461 gattttgaac aacgaatcaa ggaacgcttt cgattgtata tgaaaagcgg aatagatctt
    32521 cgagcaatga ttgattattt gcgtctttcc ttttttggga cagactacaa aacagtgatt
    32581 caacgtgtat tacgaatgga tctgacagaa tttgaaacac gtccaacggg cttgtatctt
    32641 tttgactgtc tttctgttcg tgggaatatc tgggtgttct ggcattcaaa acgaactgta
    32701 acagaaaatg tacttgtgca attttcagga caaggttgcc gggaatatga ggaagtcatg
    32761 cgtgaacaac aaacagattg gcgaacatgg ctatattcca tttggaaaaa tcaaggaaag
    32821 ttggaagctg gttatacacg tgtgcaatgt agccgattcg atattgcgtt agatgaactt
    32881 tggggattgg aaacggaaga acatttcgat ctctttgaat tactagaaaa acaaaaagca
    32941 ggtttattag aaatggactt taagagattc cagaatattg gcggttgttt tttagatgat
    33001 ggctattttc gaagtgaagg attatctttg tattttggat cgcgaaagag tcctctcttt
    33061 tttaattttt atgagaaacg ttttgaaatt gcgaaccgag aaaaaatttc agaattagaa
    33121 gcattaacga aatatggtat ttataatcga tatgagttac ggttagccaa tgagaaagca
    33181 acacaagcag tagaagcatt tattttaggt gattcaccga aacgtttagg agagattggc
    33241 gttggtttaa ttaatgcaaa acttattgtt tgggatagac tggaaagtga tgcacggatt
    33301 gttgataaac gatgggcaaa attatttcgt tcgatggaag aattggattt tacagtaaag
    33361 ccagaaaaat tctcattaga tcgttcagaa cgctggtttt tgaaacaagt agctccgaca
    33421 ttaaagttgt tacaagaagc agatcgtatt ttaggaagag actttgtaca agaagggatt
    33481 gataatgccg aactaaatga cgagcaacaa aactatctag aatggatggg aacgtcagga
    33541 gggaaaattg catgaatgca tatacgacaa cagaatggct gcgcctgttg gatttaaagg
    33601 ctgcaatcga agcattaaat gaaaagatgg ttgatttgtc ttattttcgt tttcgagttc
    33661 cttatattga acaagcggta aaagctggac gttatcagga aaaagagaac tggcaagaaa
    33721 ttgcacgctt attagaagtt cgtaagggat acgaacaaga actcgaagag ttggaattta
    33781 gccgaaggaa aggaaggcta gaattcattc gcttttatcg attttcattg ccaatacctg
    33841 cgattttagc ggttaaaaaa ggctgcgata aaatgaagat atatgaaaac tgtgtggcag
    33901 cacttagcag cgaaaaacct cttatggaag aaatttcatt ggtgactgtc acttgggaaa
    33961 tgagtcgcca gccaacagaa gaacagacat atctttcttt agaagagatt gagatagaat
    34021 tagaggaaat tggtcgctat gccacttgtt ctacctattg tggatcggtc attagtattg
    34081 ctggtgtgat tgtctaaatg aaaaaaatta tgatttatgt tggtgcgtat tggtcacgtg
    34141 atccaacagt attagaaaat ccagaggcgg tacactattg cttgcgccaa ttattctatc
    34201 tgtataaaga acgattagaa tcgttgattc gtcagttacc atatactgac agacgtttag
    34261 atgaacttct tctaaggtat ccagcaatgt ataaacgacg aaaaaataga ttgttaccag
    34321 aagagtatcc cattgaaaaa catgaacttg aagggcgatt tgtggcttat ttctatgatg
    34381 atgttcgtat gcgcttagta gaacaacgaa tggaaataga aaatgatcgc tactatttta
    34441 tcaattattg taagaagcgt aagtatcaag taactgatga tttttatgat tgtattttac
    34501 aagatgggga agtgattcta tccaaaattg ctccaagttt tcgtgaactt gtgccgattg
    34561 attttttgaa aaaatgtcat ttgcggattc ttccgtaaaa aagagctata aaaggaaaga
    34621 aaagtagaag ccaaatcggt ttctgctttt ttattttttt taggaaggag aagatggtat
    34681 gtcactggaa tttttaggag agccaattga aaaggaagag ttcgtttcgc attatatgga
    34741 tttgtttatt caggtgataa aaaactttgt gcgattgatg aattttatcc acgcgaacaa
    34801 gactatctag ccgctgaacg aataaaaatc agtttattat gggaaaaatt cacagatgaa
    34861 aataaccgac cgccagatta tcgctattta tcagattgtt taacgaataa tgtgctttct
    34921 aaagaatacc tatttcacaa acgtgagaaa aatcaaatgt cggttgaaca tttttgtgat
    34981 tgttatattg cattattgcg tagtcaacag ttattaacag tagaacgata tggtgcagag
    35041 tttggttatg tcatgcaagc tgagcgtcat agagctacaa gatgctatga aaaagaagat
    35101 caagtgattg atggctatga agaattatgt attcgaaaca atcgctattt gcaacaacgt
    35161 ttatatgaac aattgatgat ggatttttca aatctctata gagggtatga atgttcgatg
    35221 attcaagaaa ggtagaagaa aagggggaat ttttatgtgg cgattattat ttttggtggt
    35281 atgtggtatg gcattgttct atgtgtatcg tgtctatctc tatccacgaa tggaacgatg
    35341 gacaagttgg tatagagatc ctttaaaaca acgaaaagga ttgaagcggt atcgttatga
    35401 attacttgtt gaattttcgg atgggcgaca agcacagtat tatttttttg caaactatcc
    35461 aaaatacaac accagtgaac tattgattca attattcaaa aataatccat atgttgggtt
    35521 agaagaaaac ggacgggagt tcttttatcg ttcagataat attagtgcgg cgtctatgaa
    35581 caaaacacga attgcataaa aaattggagg aataatcatg aaaaaaacaa cattagcagt
    35641 cgtaacaaca ctaggaattt cattattttc gtttcaatta gggagttcat tggtttatgc
    35701 agatgaaact gtagaaacac ctaagtcaga aattgtagaa acattgccag gggaaggatt
    35761 accagatgtt gagacagagc taaatggagg aacaacggaa aaaccagcag aacctgaaca
    35821 attgcagtca gagaaacctg aaaaagagcc agtgacacca gaaccagagt ttcctgaatc
    35881 aactttaccg actgattcga tcgaagaaga aggtgtgcat agttcaacaa caacggattc
    35941 aagtatagtc acaactccgg aacaatcagg aacaaccagt tcttctgaaa agaatactga
    36001 ttcgtcaagt acggaacagc caacgatgtc gtctaccagt gaagaacaaa aagaagaacc
    36061 gaaacaacct gttgaaaaat cagaagcaat tccaaaagaa aataaaccta ttgttccagc
    36121 aaaagaagtc acagtatcgg taaccccaag cggtgaaatt acgacgaata cttctcaagg
    36181 aatgagtgta ccgattgtca ctagtaatgt agaggaattg acccatattc caacaccaac
    36241 caccccatta aaagtagcag gtgatcaaac aattgttggt gtaaaagatg gtattccatt
    36301 aatccaagat agtgaaggga atttggtaaa ggatttgtcg attcctgtaa agaaacttcc
    36361 aagtggcaat attgaaatca aaacagcaga tggaaaaaca aaagtcttac caaagactgg
    36421 tgaagaaata catgttgcac tttctgttct aggaggagtg ttgacaagta ttgctggatt
    36481 tgttggatat aaaagaaaga gaaaaggaat agaaaatgag ggtaattgaa tggactattt
    36541 ttcagaaaaa caagcttatc gttgtcaatc gtacgtacta gtaaaaacat actagaagga
    36601 gtagcttatg aaaaaacgac aactacggcg gaaagaaaaa gtttatacag agaaagtacc
    36661 gaagcaaaag aaaatccatt caaaaaaagc agtgaaatgg atttggtgtt tggttttatt
    36721 tctttgtttt tcagggggct tggcattttt tcaatccatg aaaacgaagg ttcaagtgtc
    36781 gtttttgaaa gatgaaatcc aacaattaca aaagaaagaa gtacaaagtc ggtcagaatt
    36841 aacgtacaca cctgaaattg aatcctttat gaatcgtttt gttgctttgt atatgaatac
    36901 attcgataat catgaacaag aacaacaacg tcaagaaaca ttaaaaaaag agttttatgc
    36961 acaaaacttt attcaagaac caggaagtgt ttcaggtgtt cgaaaattaa ctgctgcttc
    37021 gtttattgga ttaaaagaaa ttgaaaccat tccaactgct agttataaag taacttatga
    37081 tattcgctcg atgaatccag agagtaagaa agaaacaagt gtgacaatca cacaagtact
    37141 caatcttccc ttttctattt caaacaaggg ctgtcgtatt attgcttatc cttattttac
    37201 ggcactacca aagaatacag aaaaacaacg aatgattact tatgatgaca cattatacga
    37261 aacggttgat gcgtcggaag cgaaagatgt tgaaggattt gtggaagatt tcttagataa
    37321 gtatgcgaaa aacaatgcaa aagatatgtc atacatgatg aaagaacctg aaggactcaa
    37381 tcaaacagct aaagttgatc aagtagagat gaaagtattg cgttataaag aaacgaaaaa
    37441 accttttatt gtaaaagcaa cggtcgtctt tcgagatggt gatacgtcac ttttacaaaa
    37501 agaacaaatg acgattgtca ttacgaaaaa aacaaatcag tattatgttg aaaaattaac
    37561 acataattgg ttggatagga aaggagaaga acggaaatga cactgaaggg aattgtagat
    37621 tttatattaa cgcaaggtgg ctatttttta ataatggtgg ttgtttatct gggattgcga
    37681 aattttatga aatcaaaagt aggaaatgca ttaatatcta tcgcagcagg tgccgcagtt
    37741 tatttcttct taaatgatcc aacacgtgta tttgatgcgt ttggtaaaat cattgaaaaa
    37801 ttatttatgg attaggagga tagtgaagtg gattgggaac cttatgatta ttcgattgtt
    37861 tatgcggaac cttatcgcgt acaacagatt acaagcaatt ggcgtcttcc atttccagtt
    37921 gcattagtca atgcagcaac ttttttgttg gtcgtaatct ttgaatattt atttttacgt
    37981 tcatttattg gttgggtcat ttcttttttt ccaccagcag gagttgtttt ttatatagga
    38041 ctcccctatt tatgtgtgtt agcactggca aaactaaaac ctgatggaaa aaaagtgcat
    38101 ttttatttat gggattggtt ttgttatatg attcgtatga tgaatggtga ttgtgtgtat
    38161 tataactggg acaaggtatc aaacaatgca ttggaagaat tactttttgt gaataagtat
    38221 gtttatttag aggattagaa aaagggacaa tcaactggtt gttccttttt tattgggagg
    38281 tgaaattcat tggtgcttgt aaaagaacca gtaaaagcat tcaagaaaaa ttgtttgtta
    38341 aatggagagg attgtatctg ggcctactat catgtttttc cacgaacaat ttcaaaaaac
    38401 acaccaaaaa agacacagga aataaaagaa atgatggctc gtttactgtt gcttttagca
    38461 gattatgaag atgtggagtt ggtgatgtat ccacgaacaa tgaatttacg gacgaagttt
    38521 gacacattag gaaagggatt ttctcccgat agtcaagcaa ttggtgcgta ttatgcaaaa
    38581 gaaacagtga cattgttgga acgagaaggt caagtctatc gtccttcatt tattttagga
    38641 atcaaagtca aagaaaaagc aacggtggag agttttcatg atgtaataaa tctaatctgg
    38701 ttagatttag caggaagttt agcacgctta tttggattag aagtagatga tttggaaact
    38761 ttgttttctg aaagtgatcc ttatgtaaaa gaagtgacac aattacttta tagcttttct
    38821 attcgtccct tagtaaaaaa agagctgttg tatctcaatc gtctgcaata tattcgagga
    38881 atggctcaca aagtagaaga acaaaataat tgggaagtac tgaaagacaa tcaaggaacg
    38941 tatgtacgta taccgaagga acataaaggc cttcttcaat tagaagatga gttagggagt
    39001 agttatttag cattgttgcc tattggggaa acacctgaaa atatgagaaa ttgtgatcta
    39061 tttcaatttg ctcaggatct ttctttccca gcagaattgc gaatgaaaat tcactatcca
    39121 cgcttgaaag gacagggagg tattggttta aaactgaatt ggctatcgga acggttcaaa
    39181 acagagcata aggaattggt tcaaaatgat gaagaaccta gtgaacgctt attaactgtt
    39241 cgaggattag taaaacattt aaagcaacga gtagataatg ggcaacctgt cgtcgattgg
    39301 atcgcatgtt ttattgtata cggtcaaacg gttgaagaag ttcgtcaaaa tagtgcaacg
    39361 gttattcaac aacttcatac gattatccat atccaccgtg caaaaaatga tcaacgagaa
    39421 ttgttctata aatgtttatt agggcaacca ttgggacggc agttacattg gaagcaccga
    39481 acaactgtag aagcagttgg cgaatggttg ttttcagcag tatcagaatt aggtatggaa
    39541 tccggctggt atattggtcg gcaagataca ttaggggatg gtggttctcg aggagaaaaa
    39601 acaccattag aagaattaat ttatgcgtca aatcgttttg tctttttgaa tcttctagcc
    39661 attgcggaag gaatcaaagg tgcgttgttt gatgcgcctc atgtagcagt aactggaaaa
    39721 accggtaaag ggaagacctt tttagttggc ttgctctttt tgtatagttc gtttatgaat
    39781 gtacaaagtt tatttgtgga tcccaaagga gaaaagcgca aatggtttac gaaactcatt
    39841 caagacccgt attatcagaa acattaccct ttgtttgtag aacatttgca aaaaatgagt
    39901 tatgtcacgt tagatgctac aaatccccgt aattatggtg tattagatcc aattgtttgt
    39961 ttatcccgag tagatgcgaa acaagtagcc caggatatga ttgatgagtt aagtccgtta
    40021 ggtaccaatc accttttaaa aggggcggta ttaaagggaa tccaagaagt tttagttcaa
    40081 aaagaagccg gacaacaagt tggcttgatg catgtggtcg actatttaga acaaatggaa
    40141 caaaaagagg ttcgagaata tggtgagttt ttacgcttga cggtagaaga ttcgatttta
    40201 cgtttaggat tttcttatgg agaaaacccg ggattagact ttaatgccaa aacaacgatt
    40261 ttagagatcc aagatttgaa attaccaaat gacaatgtcc gaccagaact atatacagat
    40321 gcggatcgaa agtctttgtg tttgatgatt agtttaggtc gtttttgtga aatgtttgga
    40381 aaacgagata gtagtaagaa aacagcgatt tattttacag aagcatgggt atttaataat
    40441 tcgaacgcag gacgtagtat tattgcagca atggcacgtg tggggcgttc ccagatgaat
    40501 caattagtgt tagatacaca atttattggt gacttaggtt cagataaaga aaaagggaat
    40561 tttggtgtcg tctttgcttt tgatgaagac agtgagcgag aaaagatttt gaatcattta
    40621 cgtttggaag taaatgaagc gaacttagct gaaatgagcc acatgattaa aggacagtgt
    40681 tggatgttag atccctatgg acgtgtagga aaactcaatg tacacagttt gtttgaagaa
    40741 atgaccgctg cattacaaac aacggaaaaa acaagtcact ctcaagtaga agaacaggat
    40801 acgttaatca tgcaatagaa atgaggtgaa acaatggctt atataaagca taaggaaaag
    40861 aaatggtgcg ttttgttttt tttaacgatt ggattcggac tgctttttgt tggtggattg
    40921 gtgtatgcgg cagaaaagca ggtggatcaa tcgatctatg agattgggaa atttcatatt
    40981 gatacctttt cttcttattt atctgatagt gaattttcat taggcaaaat gttatttggg
    41041 gcaaatggca cagattttct aaaaatgatg acgaatatgt tctttagttt aagtaaattg
    41101 atttggcaag gccttgattt tgtcattgag aagctctatg aaggagctgc aatgaacgag
    41161 ttaattcacc agtttttcac tttttcggat actatttatt caaaaatata cgatcctatt
    41221 ggtgcgggga ttgtcgtctt gtatgtgtta tatctattta ctcttcgtat atttagaggg
    41281 aaacattacg caaaaatggt gtttattcga tttctcttcg ttgtcatttt ttcaatggtt
    41341 tggtttggct atggtagtaa cgtacctagt aaaggagaag cttttaccaa agggttaaat
    41401 gatttgtccg tggaggtaga aggctttatt ttttctgcta caaatagtct agatgaattg
    41461 gcgacaaaag atgtcaatgg tgttttagtg ccaacgacac aaaaggaagc cattcaaaaa
    41521 gtgcgccaac tttattataa agcggctgtt gttgatccgt atatattgtt aaattatggg
    41581 acaacaaatt tagaagaatt gtatcaagca gagattgaac cacaagaatt tctagccaag
    41641 gaagcgtcaa gtgaagaatt atctgatatt gagacaaaag tgaccactgc tgcaggagaa
    41701 gatagaggga aaaagaaggc ttatcgtgtc tatattcaac caactaaagc tgtttataaa
    41761 gcagtcgttg gttctatggt gccattgctt aatctaagta ttggattacc acttgtagtg
    41821 attggcatga ttcgttttat ctttcaaatg ttggtgttgt gtttattact tgtgattcct
    41881 ttttcattac tcgcttcttt tgttccagta tttgattata tgctcatgaa taccttaaag
    41941 tcctttttgg gcttgatgtt tcaaaaatct ttttacagtg tgattatttt ggtggcattt
    42001 ttgatctata acatagtaga cgatttaatt ccaatgaata ctgtttttgg ttttttagga
    42061 aatatgtttg tcaaagcact cctatcaatt ttactcattg taaaacggaa agatttatta
    42121 aagaaactag ggttaggagc tgcagatcaa actataaaca acacgaaaaa catagcaagt
    42181 cattctttgt ctcaaatgaa acaactgcct ggtcaactat caactgctgg acaaaatttg
    42241 gcaatcaaag gtgcaggatt agcagggaat gtgtatcgag caacgaatcc actcgcttct
    42301 gcagcgatac atggatttca aaagtttcaa caaaaacgta caccacaaga atcggtaaaa
    42361 gggaggcaag tggttaatcc ctttgaacta ggtgggcgtt caaatcaagc cttgccttat
    42421 agtggtcgtc atgttcgtta tcatggggta gagcctgaaa tgtcttctac caaatcgaaa
    42481 acagagtgga attctggtct ttcaaatggt ttgattccaa attcgtccat gaattcagta
    42541 agaccaaaac aacaggtaca atcattggca caattgagta agcaacaagg gaataatcaa
    42601 agaaaaccat tgttaaataa aggtatcgct tttgttggtc aatcgattcc gttgccttct
    42661 ggaaaagtac ccgtgccaca attaacagct gctggttctc tttcacaaca gattcgccaa
    42721 gtgaaaaatc aggcgaaaaa tgggagttat ccagcaatgc agcagttatt gaaccaatac
    42781 tctgttccta attcgtcggt agccactcta tcaggaaatc gttctgtgca acaaccgggg
    42841 caacgttcgt ctgaaagtaa accgtcgatt tccgcaccaa aatcgttaca acctgcattt
    42901 tcttctttag ataagccaac agagcaatcg gaaaaaatag aaaaggatcc taaaacactg
    42961 ccgattttgg acgcaaaacc aggaactgat tttactttac caaaaggacg aacagtccaa
    43021 aaaccattat cgaagaaagg atgaggaaga aatgaagaaa gggtgtctag gttgtcttct
    43081 tttctttatt cttccctttt ttctattgcc attctttttt tcaggagaac tttctgagca
    43141 tggaggagaa gctacaggtc tagctgtcgc ttttaatggg gaatataccg aaggattacc
    43201 gattttttca gagattaaag gacgtggtcc aatcacagat gaacatgcgc aagcagcagt
    43261 tggtgcagct gtaaagtatc agttattacc gagtgtgatt ctttctcaat taggctatga
    43321 atcagcttat ggacagtcac aatctggaag aaacgatacg aacttttttg ggattacctg
    43381 gtttcctggt tgtccctacc cacaaggttc tgcacgtgga attggtggtt ctgaaggtgg
    43441 tttctatatg aagtttccga acttaaaagc ttgttatgac tattatggct ttatggtggc
    43501 gagccaaagc aactttaatc aatgtgtggg caataaagat cctggaagtt gtttattgat
    43561 tctaggtcgt ggaggctatg cggcagcagg tattaccgaa gggagtccgt attatcgagg
    43621 agcaatgagt atcataacgt cctataactt aacggaatat gatacttttg cgatcaagca
    43681 ttggaatagc ctaccaacaa aaaaaacttc gcttattgca ggaaaaagta aaggcaatgc
    43741 gaaggttttg gatcggatca ttggacgatc cgtaaataat ggggaatgtt atggtggtac
    43801 ggcatggtat gtatctcaat taggtggccc acagttaatg ggttctggac acatgtttgc
    43861 gatgttaata ggggaagatt ataactggac aacctatggt tggcaagtaa tcatgaaccc
    43921 aaaaccaagt gatttacaag caggagatgt ggtgaattgg caagcaggtg gtgcattgtc
    43981 tcctggaatc tatggtcaca ccggaattat aacagatgtt tcatatggcg gacaacattt
    44041 ctctagtgtg gaacagaatg ccgagcaagg acggattatg gctcggtatc aacgcacgta
    44101 caatcaaaca aaaattcgta gtctggttcg taaggtgaaa taagaaaggc agtgactaga
    44161 aacgtgaaaa aagggataat tttagtattt agttggttat gtttgatagg tcttactggt
    44221 tgtcattggt tttcttctga aaaacaaaaa acacaattga gaacgacaga agaaacaatc
    44281 gaagcagtaa aagaaacaag tggaactctt caaagtactg aaagtaacag tcaaattggt
    44341 tcattagaag acgagacagc gcaacaaatg caaatagatg aaatgacaaa aaatttctta
    44401 aaagattttg ctagaaaatg gagtaatttc acagatgttt ataaacgcaa tcaagctgtg
    44461 agggactata tgacagatcg ttgtattaaa gacaatagta ttgatgtaga cccgcatgta
    44521 caaatgaaaa caactgggaa actttaccag gtgacacaag atatagagaa tccggatcaa
    44581 tatttgttat ttggagaaga aacaatgaat aaggttactc aatttgtatt acttcaattg
    44641 aaagtagatc aagaagctca aaagattgat gcgatcaaga tttattatgt acggagtgcg
    44701 tattaaaata aaaaatgaac actcatattt attatacacc gaagaaatag aaaaaaacag
    44761 actgtttttt taagaaaaac tatgtcattc tttacttgaa aaaagttgtt ataggacaga
    44821 aaggataaaa caaaaacgga aaaaatatgt cttatgaata actataagaa ctattctatt
    44881 ttttaggaga tggatacgag atgaaaaaac taattggcta tagtgtgatg tgtattggat
    44941 ttgttggtgt gggattgttt gtatcgactg aatctgcttc tgcggctgaa tggtataatc
    45001 ctatctggta taatccgaac attgaatgtc aaaaaggcta tccgtatgga ccgacaaatc
    45061 tgaaaaatgg aaaatgtaga gtaaattgga atggcgttgt agatagtgta attactggtt
    45121 caatggactc tgctctacat ggctttacgg gtcactagaa ggaggaagaa aatgtctgaa
    45181 gagaaaaaag aaatcgttga atcattagta gctttaaaag attctctttc taagattgaa
    45241 tatgttgata ggcaagaaat tcaaaaacta attgatgata ctattattga aatccaagat
    45301 gcgcgttgtg aaggtataaa aatttctgta gctttatcta aagtaataga aaaaatgaac
    45361 cgttcattag cgtttaatgg attgaagcta gatagacaaa ctagtctgat atgggatcat
    45421 ttaaaagact tgtatgacaa gtctaaaaga agtgaacgga ctgctgtatc aatcttaaaa
    45481 ggcctttggg gaatgaattc ataaacaaga tatttaaaaa gtaacttatt ttttatggag
    45541 gagaaattat gaaaagaata attaaaatat ttggtgtttt aggtgttttc ttattaggtt
    45601 taagtacttt tgttagtact ggaacaagtg tgtatgccac agaaaaagtg aatacagttg
    45661 atttagaaat aaagaataaa ttagtagatg aattaacttt ttattttgat acagttggtt
    45721 ttttagacga acatggtgta taccatataa aggattttgg tgctattcgt gaacgaatca
    45781 gtcaagataa cgaacaagga agagcagcaa gagtctttta tgaacatatt attgttccta
    45841 gaatggagaa agttgattgg aaacgatatg ctggatgtgt agccattaat tctcttcctg
    45901 gaggaggagt ggcttgggct ataggtacgc agggagattt tattgaagca ttaaaagctt
    45961 ttaattttga aaaagctgcg gatttattat taaaaattgc agaaaaacaa ttaggaaaag
    46021 ctgcagctaa agagttagct aaggtaaata ttgtagcagg aattgcagca gctgctgcta
    46081 gctgtgcatt agagtaaaag tttatgaaaa gtttagaaca agagcatatg gcttttaaac
    46141 aagcaatgtt taaagaaaat atctatttaa accataacta tattcgtgtt tctaaggcat
    46201 gttcaccagt attgaatatg cttggaggtg gaaatggatt atatcattta ctgtttgtcg
    46261 atgtttgttg gttaattttt ttaccagatg aactagttat agtcaatgaa aaaataacaa
    46321 gtaaaaatga agtgtttaat tattctttaa caagaattaa ttacaaagag attaccaagt
    46381 tttcactaga aaaagttcct ttttggggag aattttgttt aaaaattaaa tgtaactgga
    46441 aaagaatgta tttctatatt gatggtgatg atgctcttac attcggtaaa actacttttt
    46501 ctagttttaa ttttcagttt ctattaaaaa ataattttta tggtttattg aaataaggtt
    46561 gcgaaggaag atgtagagtt atttgcactg ttgtgttggt atatctctta atgctgtgtc
    46621 tttttgggga gattaattag tggagtgatt atatgagtat agatgattgt ttagaagaac
    46681 aaataagatt atttttaaag tttttaaaag ttaaagttca aaataactac ttcacatgtt
    46741 atagttatgc acaccctgtt ttaaatagtc ttccttttaa ttctttagtg caaggattga
    46801 ttacaaatat tgttttatta gtctgtacac caaatgaaat atttgtaaaa aagataggca
    46861 gtggtatttc ttcagtaaga ttaaaagaga aagaatatac tcaaaagatt attcatattc
    46921 ctaagcctga aattaagcgt tttgagataa aagactattc tgctttaggg ctgttctcaa
    46981 ttggttactg gtgataaaaa ataagaatac taaaactata cgtagaatat aagtaatcgg
    47041 catcaatcct aattatgtga ttgaatgccg atttttgtta cgcgctattt ttaattatct
    47101 gtaagaagaa taattttatt ttactcatat tcgttttcac tttgaagaag taaattgcaa
    47161 tttcttataa gttaagcagc tatgcaaaag gctatttatt ctattgtgaa atataagatg
    47221 tagttgaaac aggatatata gaatcaaaat acgaatctta ttttgatttt tctagttttt
    47281 tgaaaaaagc accctttgat cgttgtatta gtggaaaaga gaaaaaggag catacttatc
    47341 atgtcacaaa actacaacta aatatctaaa caacaccaat ttgatgccta ttgaaaaaaa
    47401 gtattgcgaa atgaagccaa gtctattaga aaatgaaata atagaataaa agaaaatggg
    47461 aaaccgctga atgatgtaaa tgaaggtaag tatccacgaa ctaattttga tgaacaagat
    47521 atctattttt tatttgtatt ggagatacta gtttaatatc aaaagttatc agcagtcata
    47581 attagttatc caatgtaaaa agtacaaaca atagttgtta tgcagaatag tttatgaaat
    47641 atattacata ataagaaaac aagcgatatt tttctgatca gaacaaccat ttaatttaaa
    47701 aagagttaaa agttatatat ttttgaaata ttttggatga gtagctaaaa caatattttg
    47761 ctcattaagc attaaatgaa gatgcaacga tgttagaaag ttagcttctt ctggatcttg
    47821 ttttgttaat gcctcaaata gttgataatg ttgttccata attttttccc actttaatcc
    47881 ttccgttttt actcgtagcc atcgaaatcg ttctaagtgt gtattgttat tttctaacca
    47941 ttgccatact tcttctcgct ctgcaatttc aaaacaagct ttatggaata aattatcatt
    48001 atgaaaaaaa ctacgagcat ttttttgact aacagctttt tcttgttctg aaattatagt
    48061 ttctaaaatc cgtttttttt ggttattcat tttggcacaa cactctaaca taatttgtcg
    48121 ttccaaatgt tctctgacaa atcgagcatt tttggccgaa tctaaatcaa ttaatgagat
    48181 ataatttccg ctttgaggaa ttgtatcaac aagaccttgt tgtcggagtt gaatcagtgc
    48241 ttcacgtatt ggtgtacgac caatagaaag tgtttcttct aatttttttt ctgatacttt
    48301 ttttccaggt tctaagtcag catagataat ttgtcggcga atagattggt aagcttgatt
    48361 ttggagacta gttttaattg tcatacacaa tgctccttaa cttttatttt aactgatatt
    48421 ttaattctgt tcatgccata taatatcaaa ttttgaagta atacactagt tataaaaata
    48481 aaagaataca tgaaaaaaag aaaaaataag tttcgataaa atcagtccaa aaagaaggag
    48541 tattttattt ttctgaataa aaaatacaat cattctgata tggtagaaaa aagcaagttt
    48601 attccctttt tatattgaca acggttacaa tgggttttaa aatataaaat gtatcaaaac
    48661 taacatatca gtttgaggag gttaattatg gtttcgatta aaaattggca aaattctatc
    48721 gaagaattca atgaaatgca gattctactg ccgtgttttg atgtcaaaga agttaagcaa
    48781 gaaggccagc aacatccagt atggcttcat tttggtggag gaaatcttta tcgaggattt
    48841 catgcagaaa ttgctcaatg cttagcagat caaaatgaat tagaaacagg agtagttgtg
    48901 tgtgaaacat ttgatgaaga agtagtggaa aaagcttatc atgactataa caatgacatt
    48961 ttggaaattg ttatgagtgg agatggtgaa ctcaaaaaaa gacttttggc agtaacggct
    49021 gatagtttat attgcaatcc gaagaatata gcggactatc aaagagtggt taaatatttt
    49081 gaagatccta aattacagtt tacaacgttt actattactg aaaaaggtta cagtttaaaa
    49141 gattctaaag ggcaattctt ttcggtagtc aaagctgata tcaaatctgg acctagcaat
    49201 cctcaacata cgatgagtat tgtaacatcc ttgttatata gcagatttaa tgctggagag
    49261 ttgccgattg cgatggtttc tactgataat ttctcacaga atggtcaacg ttttcaagat
    49321 agtattttta cgattgcaga agcttggcaa aaagaaggat atgtttctga aaaatttatt
    49381 gcttatttaa aggataaaga aaaagtgact tttccttgga gtatgattga tcgaattacg
    49441 ccaaatcctt cagagaatgt aaaggaagat ctattgatga tggggctcga ggatttagat
    49501 atcattcaca caaaaaaaca taccaatatt gctgctttca ctaatactga ggtagttcat
    49561 tatttagtta ttgaagatag ttttccaaat ggccggccta aattggaaaa ggctggtgtg
    49621 attatgacag atagagagac ggttgataaa gcagacatca tgaaggttac gacaagcttg
    49681 aatccacttc atactgcttt ggcagtaaca ggttgtctgc taggatatca atcaatttca
    49741 gcagagatgc aggatgatga tttggttggt ttaatcaaag gaataggtta tttagaaggt
    49801 ttaccagtag ttgaaaatcc aggcattatt gatccaaaac aatttattga tgaagtaatt
    49861 aataaacgtt tgcctaatcc ctatattcca gatacaccac aacgaattgc aacagatact
    49921 tctcaaaaag tagcgattcg ctatggtgaa acaattaaaa agtatgtcga aaaagacgga
    49981 aatgcagaaa atctaacatt tattcctatc gctattgctg cttggttacg ttatttactg
    50041 gcagtcgatg atcaagggga aaattttgtg ccaagtcctg atccgttatt ggtagagttg
    50101 caggataaat tatcagtatt gaaattaggt gaacaagatc cttcaaagat tcattgtgcg
    50161 gtacaggaaa ttttagaaaa taaagaaatc tttggtttag atttatatca agttggggta
    50221 ggaaagaaaa ctgaaaaaat cttttgcgaa atgctagtag gtcctcaagc agttcgtaaa
    50281 acaatcaata aatatataac agaagttgga ggaaagtaac tatggaaatg acttttagat
    50341 ggtatggggc aaatgaagaa aaaatctcac tacaagaaat tcgtcagatc ccagcaatga
    50401 aaggaattgt tggaacatta atggatatac cagccggaga ggtttggccg agagaacgaa
    50461 ttaaatcttt aaaagatgaa gtcgaagcca atggacttac cttgaaagtt attgagagtg
    50521 ttaatgttca tgatgatatc aaaattggtt taccaacacg agatcaatac attgagaatt
    50581 ataaacagac gattcgcaat ttggcagaat ttgggattga ggttatctgt tataatttta
    50641 tgccagtatt tgattgggta aaatcggatt tggattatcg tttagatgat gggtcttcta
    50701 cgttagccta tatttcaaaa aatatcccta ctgatccgga ggaaattatt agagaagtag
    50761 aaaattctac taataatttt gaattgccgg gttgggaacc tcatcggtta tcacaaatta
    50821 agcaattatt agaagcctat aaagaagttg atgaagagaa attacgagag aactttgcat
    50881 attttctcaa agcaattatc ccgacatgtg aagaagttgg gattaaaatg gcagttcacc
    50941 cagatgatcc accatattcc attttcggat tacctcgtat tgttaaaaat cgtgacgatt
    51001 tagattggtt gtgtaatgta gtggattctc cttctaatgg tattacatta tgtactggaa
    51061 gtattgcaga acgaccagat aatgatgtct atgaaattct atctgaattt tgtcaacggg
    51121 atcggattcc ttttgcacat gttcgtaata tcaaatattt aagtgacaat ggactcgatt
    51181 tttacgaagc gccgcataaa tcagaatatg gttctctaga tatgtataag atattaaaaa
    51241 caatgtacga taatggattt aatggctata ttcgtccaga tcatggtcgg atgatttggg
    51301 gagaaaccgg tagacctggt tatggattat atgaccgagc tctaggtgca gtttatttaa
    51361 atggtatttg ggaagcgctt gaaaaggggg cgagtaaata attatataag ggagtttatt
    51421 tagaggaaaa attttctctt ctaaaaacta acggaaggaa aggagtgttt aaatatgttt
    51481 ttagataaga actttttatt agagaatgaa atagctaaaa gactttttca taaatttgca
    51541 aaacaccaac caattatcga ctatcattgt catttagacc cagcatattt gctagaaaat
    51601 aagcaataca agaatttgac tcaggtatgg ttaaatgata aaggagctgg agatcattat
    51661 aaatggcgat tgatgcgagc taatggtata gaagaagagt tgatcagtgg tccaggtgat
    51721 gattatgaaa aatttttagc ttttgtaaaa acagtggaga aggcgccagg taatcctatt
    51781 tttgaatggt ctcatttgga attacaacgt tactttgata ttgatttgac aatttctgag
    51841 aagaatgcac cagaaatttg gcgacgtgct aacgaaaaaa ttcaaacgga ttcctatcgc
    51901 ccgaaagaat tgttggagat gatggatgtg aaagccatct gtacaacgga tgatcctaca
    51961 gatgacttgt ggtatcatac tgagctttct aaacaggata attctttcaa ggtattacct
    52021 actttccgtc cagataaggt ctggagtatc aaatcatcag gcttttcgaa ctatatttta
    52081 aaacttagtc aaacagcggc tataccaatc aaagacttta atgggttgaa gcaagcattg
    52141 agccaacgag tggatcattt tcatcaaatt ggctgtcgat tagcagatca cggtttgaat
    52201 acttattttt atgaaatagc agatgaaaaa atactcgaca atctgttaat aaaagcttta
    52261 gcaggaaaaa cggatttcca tttatcagaa attgcacaat ttgttacagg tattcaatta
    52321 catttgatgt ctgagtatac aagatatggt tggacgatgc aaatgcattt aaatgcttta
    52381 agaaataatt caagtatatt aaaggaacaa attggagtag atgtcggtgg tgattcgatg
    52441 ggggatcaag ctagtttgac acaaaatgtt gtagcattgt tcgataccgc tgaaaagagt
    52501 gggtgcttac caaaaacaat tttatattca ttaaatccaa ctgattggct tccactagca
    52561 acagcaatgc aaagcttcca agggggtatg attcaaaaaa tccaattggg atgtgcttgg
    52621 tggttcaatg atacagctga aggaatgcgt caacagttga ctacgatggc acaacaaagt
    52681 ttgttgtcga attttacagg tatgttgact gattctcgaa gttttctaag ctatcctcgc
    52741 catgagtatt ttcgtcgagt attatgtcaa ttactaagcg agtgggttaa agcaggccga
    52801 attccagatg atgaaatgta tcttagtcag atagtggaga atatttgtta tcgtaatgcg
    52861 tataattatt ttaatttttt tgaaatcgga agtagaaaat aatacttgaa ataaaagctt
    52921 aaatttattc tttgtaagtt agtacagcta gacaaagtat gtatccataa aggaaggaga
    52981 tagatatgaa aaaagtagaa attttaagta agttagaagc ggctggagtc attgcggttg
    53041 tacgtgggaa aaataaggat gaggccatta aagcaataaa ttcaattatt gcgggtggaa
    53101 ttaaagggat tgaactaact tttacagttc ctaatgctga agaaataatc aaagaaattg
    53161 ctaaaaaata tgctgatcaa aaagatgtaa taatcggagc cggtacggtt ttagatgcgt
    53221 ttactgctcg tatcgcaatt atggctgggg cagaatatat cgttagtcca tcttttgatc
    53281 gacaaacagc tgagatttgt aatttatatc aaattcctta tttaccaggt tgtatgacaa
    53341 ttactgagat aaaggaggca cttactgctg gcgtagatat cattaaacta ttcccaggtt
    53401 ctgcttttgg tccatccatt gtaaaagcct atcaagctcc attaccacac gtaaatataa
    53461 tgcctactgg cggagtaagt atagagaact tagaagattg gtttgaagta ggagttgttg
    53521 cagttggtgt aggtgggaat ttattggctt ctgttgaaaa aggtgatttt gaagaagtaa
    53581 gtcgagtagc tagtaaatat atagataagt tttatgagat aaagaattat taggagaaaa
    53641 aacaaataat actctgaaat gtaaaaaata aatactgact ctttacgctt cagagtatag
    53701 tacgtacttt catcagaatg aaaacgctta acaacagaag ggagagaatt gtatggaaag
    53761 aaaatttggt ataagagaca aaataggata tatgtttgga gattttggaa acgattttac
    53821 tttcattttt gctagttctt ttttaatgat cttttataca aaagtattgg ggataagcgg
    53881 agctgccgtt ggaacattat ttttagtagc aagatttatc gatgcagtta ctgatgtaac
    53941 tatggggcgt attgttgata gatcgaaagc aactaagcag ggtaagttta aaccgtggat
    54001 tttaagaatg agtggtccag tcgctttagc aagtttttta atgtatcaaa cttctatgat
    54061 ttcagcttct atgagcgtta aggtagtata catgtatgtt acgtatatat tatgggggag
    54121 tattttttac acatctataa atattcctta tggatcaatg gcatcagcta ttacttctat
    54181 tacagaagaa agaacaatgc tatcgacatt ccgaaatgtt ggagctactt tagcaagttt
    54241 ggtcattggt gtattaacac cattatttat ttatacaaga gatgattcag gcgctcaaat
    54301 agttcgtggt ggatcaactt ttacaattgt tgcgggaata ttttcgatat gtgcacttat
    54361 ttgttattta atatgttata aactaactac tgaacgagta agaattgacc aatctcaata
    54421 caaagtttca ttgttaaaat ctttaaaagg gttagttaaa aatagggcat tactttctat
    54481 aattggtgcg gctattttct tactccttgc tcaattatta attatgagta tgaataatta
    54541 tgtatttcct gattactata atgatgcaca gggaatagtt ttaatgaatt ttatcaatcc
    54601 tattttggtt ttaatagttg tttcgccttt aactttgttt ttaagtaaaa aatatggaaa
    54661 aaaagaactg gcatcagtag gaatgttctt ttcagcaata gtatatttga tcttattttt
    54721 gataaaagct agtaatatgt atgtattctt aattcttagt tcggttggct atatgggttt
    54781 gggtgtgttt aatactgtta tttgggcaaa tattactgat gttattgatg atcaagaagt
    54841 aaagtctgat caaagagaag atgggaccgt atatgcggta tattcttttg ctagaaaaat
    54901 aggtcaagct ctagctggag gcgtcggagg ttggactctt tctattatag gctatgactc
    54961 tttagcaaat gtacaaacgg gagatgtttt agaaaagtta tataacgctt caacattaat
    55021 tccatcaatt tgttttctga ttgtaggatt aattttattt ttcctttatc cattatctaa
    55081 agataaagtc ttagaaaatt gcgagctttt aaaacaaaaa cataaatagg agagaaatat
    55141 gaataattgg atattttgta ttgattcaga tggctgtgtt atggatacga tgacttataa
    55201 acacaatctc tttttcggac caattgctgc agagattttt gatgttcatc atagagaaaa
    55261 attcctacag gattggaata atattaattt atattcagag actcgtggaa taaacagatt
    55321 tttaggttta gttaaaacat tagaaaaaaa tgatgtaaca tcgattaatc gattaaagca
    55381 ctgggtatct ctaacaaaag aattgtctaa tccatctttg aagagggaaa tagaatattc
    55441 tcagtcagag gatttattaa aagctttaaa atggtcggaa gaagtcaatc aacagattag
    55501 tcaagcaaaa ggattagata aacccttttt aagtgctaga gatactataa aagctctgaa
    55561 aaaatatggg aagataatta ttgtaagctc agctaataag gaagctgttc aagaagaatg
    55621 ggaaagacat gaattattat cattagttga tgaactgtgt tgtcaagata aaggaaaaaa
    55681 agaagatatt attcgaagtg taattgaaaa tggttgtgat ttggataaga tattaatgat
    55741 tggtgattct cctggtgatc tagaagctgc aaataaaaat aaagttttct tttatcctat
    55801 tttggtcaat aaagaaaaag aatcatggga aaacttaaga actgatgttt tatttaaatg
    55861 tttatcggga gattatgtag atattgaaca aaaatatata gatacttttt ggaaaaatct
    55921 tactaataag tagaggagaa agaaaatgac tataaaagta gatttacgaa aaaaaccata
    55981 taatttagat gaagatgcta tttcttggat tgaagagacg attgattcta tgactttgga
    56041 agaaaaaata ggtcaattgt ttgttaatat gggatctagt cgtgataaaa attacttgag
    56101 taatatggtc aatcagtacc atattggagc tgtacgatat aatccaggaa ctgctgatga
    56161 ggtatatgat caaaactata tcttacaaac agaaagtaaa attcctctat taattgctgc
    56221 taatacggaa gcaggtggta acggagcttg tacagatggt actgaagttg gtgtagaagt
    56281 aaaaattggt gcaacaaatg atgcgaaata tgcttatgaa ttagggagag tttctgggat
    56341 tgaagcatcg gctattggat gtaactggag ttttgctcct attgtagata ttaatagaaa
    56401 ttggagaaat cctatcattt ctagtcgatc ttttggacaa aaccctgata aagttttaga
    56461 acttagttta gcttatatga aaggtattca ggagagtgga attttgcctg cagctaaaca
    56521 tttccctggt gacggtattg acgaacgaga tcagcatcta tcttttagta ttaatagttt
    56581 aggtacggaa gaatgggata attcttttgg aaaagtatac tcaggcttaa tagaagctgg
    56641 attaccttca attatggcag gtcatatcca tatgccagct tattcaaaac attttaatcc
    56701 tgatctgaaa gatgaagatt tacttcctgc aactttatca aaagaattaa tcactgactt
    56761 acttagagga aaactcggat ttaatggatt agtagttact gatgctagtc atatgttagg
    56821 aatgactggg gcaatgaaac gatcagaatt attgcccact tctattgcag ctggatgtga
    56881 tctatttcta ttttttaatg atccagatga agactttaat tatatgatgg aaggctatcg
    56941 taaaggaatt atcactgatg atcggttatt agatgcctta aaaagaattt tagggttaaa
    57001 agctaaattg ggattacaca aaaaagcaaa agaacaaatt ttattaccta agcaagaagc
    57061 tctggaaaaa attggtttaa ttgaaaataa atcaattttt aaagaggtgg ctgaccaagc
    57121 aattacatta gttaaagata aacaagatat ttggcctatt gtaccagaaa aatataaaag
    57181 gattttacta gtagatgtac atggggtaaa aggtgggttt ggtgaagtag ttgctggaaa
    57241 tagacctcaa gccattgatt tattgaaaca gattttgtca gcaaaaggat ttgaagttga
    57301 aaaatacatt tcacctgaag aagaattgaa aggattatcg caagaagaaa aaattaaaaa
    57361 tatagctgga atatatgctg ctaagagacc aattaaaaat ttaactaaaa aatatgactt
    57421 gatcattaat atagctaatg ttaatccagg cacagttcaa cgaattgtct ggccagcaag
    57481 taaaggaact ccggatatac cgttttacat tcatgaaatt ccaacaattt ttgtatctgt
    57541 acagtgtccg tttcatttgg ctgatgtacc tcaagtaaag acttatatta atacttatga
    57601 tggaaaacaa gaaactttag aaatattggt ggacaaacta atgggtcttt ctgaatttaa
    57661 gggagtaagt ccaatagatg cttattgtgg tttaaaggat acatttattt aatatttaga
    57721 taggagtggt ttatatgttg tatcccatta tgacagagac gaggcaagta attgacttaa
    57781 gcggtctctg gagatttaaa ttagatacaa atgatgactt acaggaagaa cttgctttag
    57841 aaccaataaa tgaaaaagag agttatccga tttcagttcc ctcttcttat aatgatttat
    57901 ttgaaacaga gaacattaga aatcatgttg gatgggtgtg gtatgaaaaa gaagtgactc
    57961 ttcctaaaag attattagaa gaaagattag ttcttcgttt tggatctgca acacatgaag
    58021 caaaagtttt tttgaatgga aagtttatta ctcaacacaa aggtgggttt acaccatttg
    58081 agtctgaaat taatcggttt gtaaaagacg ggaataatcg aattacagtt gctgtaaaca
    58141 atattattga cgatagtact ttacctgtgg ggattatgaa ggaaacagta caggaaaatg
    58201 gggatataaa aaaggaaaac ttagttaatt ttgatttctt caattatgca ggtattcata
    58261 gacctgttaa gttatataca actccgaaag actatataag tgatattact attaatacaa
    58321 gaataactga tttggatgca agtgtagaat tcaatatcga tacagaatgg accggaaaaa
    58381 tcttaatagc gatatatgac gaagaggctc aacttgtagc tgaagctgaa ggaaagaaag
    58441 gaatgattca gcttaagaat ccaatttttt gggaacctct agacgcttat ttatatagta
    58501 tgaaaattag tttaattgat gataataaag tgatcgatac ctatacagaa gaatttggta
    58561 ttcgcagtgt tcaggttacc aatggcaaat ttttgattaa taataaacct ttttatttta
    58621 aaggatttgg taagcatgag gattcgtatg ttaatggacg tggtttgaat gaggctgtaa
    58681 atattaaaga ttttaattta atgaaatgga taggtgctaa ttcttttaga acatcacatt
    58741 atccttactc agaagaaatt atgcgattag cagatcgaga aggtattgta gttattgatg
    58801 aagtacctgc agtagggtta catttaaatt tcatggccac tttattagat gataatgtgg
    58861 aaaaacataa tacgtggaaa gagatacgaa cagaaaattc tcataaaacg gtgatcaagg
    58921 aattgataga acgcgacaag aaccacccaa gtgttgtaat gtggtctatt gcaaatgaac
    58981 cagattcgga ttccgaaggt gctaaagaat attttgaacc tttagttgaa ttaactaaag
    59041 aacttgatcc gcagaaaaga cctgtaacaa tcgttactta tctaaaatca acgccagatg
    59101 tttgtaaggt aggagatatt gtcgatgttt tatgtttaaa tcgttattat ggttggtatg
    59161 tttcgggagg ggatctggca catgcaaaat atctgttgaa aaaagagcta gatggatgga
    59221 tggaaagatg tcctgatacg ccaataatgt ttacagaata tggagcagat acagtagctg
    59281 gatttcatga tacagtacca acaatgttta cagaagagta tcaagtggct tattacgaag
    59341 caaatcacga agtaatagat acatgtgaaa attttgttgg tgaacaagtg tggaattttg
    59401 cagattttgc aacaagtcaa gggattttaa gagtgcaagg aaataagaaa ggaatcttta
    59461 ctagagatag aa
//
LOCUS       DACCKU010000006        54604 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.006, whole genome shotgun sequence.
ACCESSION   DACCKU010000006 DACCKU010000000
VERSION     DACCKU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 54604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.006"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(136..843)
                     /gene="deoD"
                     /locus_tag="IVN13_000337"
     CDS             complement(136..843)
                     /gene="deoD"
                     /locus_tag="IVN13_000337"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7010857.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(860..1678)
                     /locus_tag="IVN13_000338"
     CDS             complement(860..1678)
                     /locus_tag="IVN13_000338"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP7010858.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IVN13_000339"
     CDS             complement(1702..2868)
                     /gene="deoB"
                     /locus_tag="IVN13_000339"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP7010859.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            complement(2967..3923)
                     /locus_tag="IVN13_000340"
     CDS             complement(2967..3923)
                     /locus_tag="IVN13_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7010860.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            complement(3923..5056)
                     /locus_tag="IVN13_000341"
     CDS             complement(3923..5056)
                     /locus_tag="IVN13_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7010861.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            complement(5049..6611)
                     /locus_tag="IVN13_000342"
     CDS             complement(5049..6611)
                     /locus_tag="IVN13_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7010862.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            complement(6823..7905)
                     /locus_tag="IVN13_000343"
     CDS             complement(6823..7905)
                     /locus_tag="IVN13_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7010863.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            complement(7971..8369)
                     /locus_tag="IVN13_000344"
     CDS             complement(7971..8369)
                     /locus_tag="IVN13_000344"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7010864.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IVN13_000345"
     CDS             complement(8390..9052)
                     /gene="deoC"
                     /locus_tag="IVN13_000345"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7010865.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            complement(9098..10399)
                     /locus_tag="IVN13_000346"
     CDS             complement(9098..10399)
                     /locus_tag="IVN13_000346"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7010866.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            complement(10540..11556)
                     /locus_tag="IVN13_000347"
     CDS             complement(10540..11556)
                     /locus_tag="IVN13_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7010867.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            complement(11864..12469)
                     /locus_tag="IVN13_000348"
     CDS             complement(11864..12469)
                     /locus_tag="IVN13_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7010868.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12665..13588
                     /locus_tag="IVN13_000349"
     CDS             12665..13588
                     /locus_tag="IVN13_000349"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7010869.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            13647..15212
                     /gene="guaA"
                     /locus_tag="IVN13_000350"
     CDS             13647..15212
                     /gene="guaA"
                     /locus_tag="IVN13_000350"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7010870.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(15280..16473)
                     /locus_tag="IVN13_000351"
     CDS             complement(15280..16473)
                     /locus_tag="IVN13_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7010871.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(16500..16700)
                     /locus_tag="IVN13_000352"
     CDS             complement(16500..16700)
                     /locus_tag="IVN13_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7010872.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            complement(17198..17428)
                     /locus_tag="IVN13_000353"
     CDS             complement(17198..17428)
                     /locus_tag="IVN13_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7010873.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            complement(17425..17847)
                     /locus_tag="IVN13_000354"
     CDS             complement(17425..17847)
                     /locus_tag="IVN13_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7010874.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            18378..18731
                     /locus_tag="IVN13_000355"
     CDS             18378..18731
                     /locus_tag="IVN13_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7010875.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            complement(18789..18977)
                     /locus_tag="IVN13_000356"
     CDS             complement(18789..18977)
                     /locus_tag="IVN13_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7010876.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(19075..20115)
                     /locus_tag="IVN13_000357"
     CDS             complement(19075..20115)
                     /locus_tag="IVN13_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7010877.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            20477..20974
                     /gene="dfrG"
                     /locus_tag="IVN13_000358"
     CDS             20477..20974
                     /gene="dfrG"
                     /locus_tag="IVN13_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7010878.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(21046..22998)
                     /locus_tag="IVN13_000359"
     CDS             complement(21046..22998)
                     /locus_tag="IVN13_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7010879.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(23005..23301)
                     /locus_tag="IVN13_000360"
     CDS             complement(23005..23301)
                     /locus_tag="IVN13_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010880.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            complement(23476..24279)
                     /locus_tag="IVN13_000361"
     CDS             complement(23476..24279)
                     /locus_tag="IVN13_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7010881.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            complement(24295..24411)
                     /locus_tag="IVN13_000362"
     CDS             complement(24295..24411)
                     /locus_tag="IVN13_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP7010882.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(24656..25585)
                     /locus_tag="IVN13_000363"
     CDS             complement(24656..25585)
                     /locus_tag="IVN13_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7010883.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            complement(25602..26624)
                     /locus_tag="IVN13_000364"
     CDS             complement(25602..26624)
                     /locus_tag="IVN13_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7010884.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            complement(26621..28648)
                     /locus_tag="IVN13_000365"
     CDS             complement(26621..28648)
                     /locus_tag="IVN13_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010885.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            complement(28645..31098)
                     /locus_tag="IVN13_000366"
     CDS             complement(28645..31098)
                     /locus_tag="IVN13_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7010886.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            complement(31082..31477)
                     /locus_tag="IVN13_000367"
     CDS             complement(31082..31477)
                     /locus_tag="IVN13_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7010887.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            complement(31549..32187)
                     /locus_tag="IVN13_000368"
     CDS             complement(31549..32187)
                     /locus_tag="IVN13_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010888.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            complement(32243..32737)
                     /locus_tag="IVN13_000369"
     CDS             complement(32243..32737)
                     /locus_tag="IVN13_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7010889.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            complement(32804..33583)
                     /locus_tag="IVN13_000370"
     CDS             complement(32804..33583)
                     /locus_tag="IVN13_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP7010890.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            complement(33625..33846)
                     /locus_tag="IVN13_000371"
     CDS             complement(33625..33846)
                     /locus_tag="IVN13_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010891.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            complement(33843..34133)
                     /locus_tag="IVN13_000372"
     CDS             complement(33843..34133)
                     /locus_tag="IVN13_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010892.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            complement(34130..35314)
                     /locus_tag="IVN13_000373"
     CDS             complement(34130..35314)
                     /locus_tag="IVN13_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7010893.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            complement(35496..36899)
                     /locus_tag="IVN13_000374"
     CDS             complement(35496..36899)
                     /locus_tag="IVN13_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7010894.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            complement(36921..37694)
                     /locus_tag="IVN13_000375"
     CDS             complement(36921..37694)
                     /locus_tag="IVN13_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010895.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            complement(37704..38081)
                     /locus_tag="IVN13_000376"
     CDS             complement(37704..38081)
                     /locus_tag="IVN13_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7010896.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            complement(38102..38416)
                     /locus_tag="IVN13_000377"
     CDS             complement(38102..38416)
                     /locus_tag="IVN13_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7010897.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            complement(38616..40409)
                     /locus_tag="IVN13_000378"
     CDS             complement(38616..40409)
                     /locus_tag="IVN13_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7010898.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            complement(40406..42595)
                     /locus_tag="IVN13_000379"
     CDS             complement(40406..42595)
                     /locus_tag="IVN13_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7010899.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            complement(42729..43919)
                     /locus_tag="IVN13_000380"
     CDS             complement(42729..43919)
                     /locus_tag="IVN13_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP7010900.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(44791..46422)
                     /locus_tag="IVN13_000381"
     CDS             complement(44791..46422)
                     /locus_tag="IVN13_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP7010901.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(46436..48109)
                     /locus_tag="IVN13_000382"
     CDS             complement(46436..48109)
                     /locus_tag="IVN13_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7010902.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            complement(48122..48439)
                     /locus_tag="IVN13_000383"
     CDS             complement(48122..48439)
                     /locus_tag="IVN13_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP7010903.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            complement(48464..49864)
                     /gene="lacG"
                     /locus_tag="IVN13_000384"
     CDS             complement(48464..49864)
                     /gene="lacG"
                     /locus_tag="IVN13_000384"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP7010904.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            50101..50886
                     /locus_tag="IVN13_000385"
     CDS             50101..50886
                     /locus_tag="IVN13_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7010905.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            50889..51581
                     /locus_tag="IVN13_000386"
     CDS             50889..51581
                     /locus_tag="IVN13_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7010906.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(51855..52226)
                     /locus_tag="IVN13_000387"
     CDS             complement(51855..52226)
                     /locus_tag="IVN13_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP7010907.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(52626..52856)
                     /locus_tag="IVN13_000388"
     CDS             complement(52626..52856)
                     /locus_tag="IVN13_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010908.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            complement(52950..53288)
                     /locus_tag="IVN13_000389"
     CDS             complement(52950..53288)
                     /locus_tag="IVN13_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010909.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            complement(54216..54332)
                     /locus_tag="IVN13_000390"
     CDS             complement(54216..54332)
                     /locus_tag="IVN13_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7010910.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
BASE COUNT        16208 a        11105 c         9230 g        18061 t
ORIGIN      
        1 ttcacaacct cttggtaaac ggtgttcaaa agcttacatt tgcaataata agtccagcca
       61 agtcaaaaac atgaggagct atttttgctt ggatgttctt attacttaat gcagggcagc
      121 tttttcattt ccttcttatt ctgctatcgc tgtttccaat cctacttcga tcatttcatt
      181 gaacgtagat tgacgttctg cagctgttgt ttcttcaccg gtgatcaaat ggtcgctaac
      241 ggtcatcaaa cttaatgtct gcacatggta tttcgcgcct aagtaataaa gagccgctgc
      301 ttccatttcc actcctaaaa cgccaagttc agctaattgg aatgtctctg tcaaatcatc
      361 tttgtagaaa gaatcttccg aaagaacatt tcctacatga accgtatagc ccttttcttt
      421 agctatctca tatgctttca gtaatagatt gaaatcacta attggaggaa aatgaaaggc
      481 ttggaaattt ttttcgatca tcgaagaact ggtcgctgca ccttgtgcta gtactaaatc
      541 acgaacatgg acgtcttttg acaatgcacc gcatgtacct actcgaatca atttctttac
      601 accatacgac tggatcaatt catgggcata gatggtggct gatggcattc ccattcctgt
      661 tccttgaaca gaaatacgtt ctcctttata tttgccggta aagcctaaca taccgcgtac
      721 ctgattgtaa caaactggat cttccaaaaa agtctccgca atatatttag ctcgcaaagg
      781 atctcccgga agtaaaatct tatcggcgat ctcgccttct ttcgcttcaa tatgcacact
      841 cattttattg ttcctcctat tacaataatg tcaatgtttc tttaaccaat gctttaaaag
      901 attgtttgac tctctgagtt gtttctacaa cttcttcatg gctcagattc gcttgcatac
      961 ctgctgctaa gttggtaata caagagattc ctaacacttt taatccgctg tgagctgcta
     1021 cgatgacttc tggaacagtc gacatcccta ctgcatcggc tcctattgtc cgagacattc
     1081 ggatctccgc aggcgtttca taagttggtc cagaatagcc catatatacg ccttctttaa
     1141 gatccaaacc tagtttttct gctgctgctt ttgctgttgc ttgatagtca ggcgtataag
     1201 cttgactcat atctggaaat ctagggccca ttgcatcttc attcggaccg atcaaaggat
     1261 tttcaccagt aaaattgata tgatcggtaa tcagcatcaa atctcctggt acaaatgatt
     1321 cgttgacacc accacatgca ttagtcacaa tgactgaatg ggctcctagc ccggccatca
     1381 cacgtactgg ataggtcact gtttgcatgg aatgaccttc atagtagtgg aatctccctt
     1441 gcatggctaa cactttcttg cctgccagag acccataaac tagctttcct gcatgcccaa
     1501 ctactgtcga aacaggaaag aacgggatct catcataaga aatcattaca gcatcttcga
     1561 tttcatctgc taattcacct aatcctgaac ctagaatcaa accaaaatca atttctttta
     1621 ctcctttatc aaggataaac tgggtcgttt cttgtaacat ctcgcttaac ttggtcattt
     1681 gctttctcct atttaaaaag attattttaa taatttcaag aagctttcgc cattttcagt
     1741 cgaagaaaca ctaaagttct cagcaatcgt tgctgaaata tctgaataat atccttgagg
     1801 caatgcccct tgttctttca tttttttact gtaaaccaat aacggtacat attctcgcgt
     1861 atgatcggta ccagtaaacg tcggatcgtt tccgtgatcg gctgtaatca ttagcaagtc
     1921 gtcttcttcc atcgcttcaa gcaattctgg aattcgtaag tcaaattctt caatcgcatg
     1981 cgcatagccg actacatccc gacgatgacc gtataatgca tcaaaatcta ctaaattggt
     2041 aaagctgatt cctgaaaaat cgtttttcat aactttcagt agctggtcaa cgccatccat
     2101 attgcttttc gtgcggacag attcagtgat tccttgtccg ttgaaaatat cgttgatctt
     2161 tccaacagca atcacgtcct ttcctgcttc tttcaaagaa tcaaggactg tgtgtccgaa
     2221 tggattcaac gcataatcat ggcgattgct tgttcgtgtg aagtctcctg gttcacccac
     2281 gtacggacgt gcaatgattc ggccaatcat atacggatca tcttttgtta tatcacgaac
     2341 gaattggcaa atccgatata attcttctaa aggaatgatt tcttcatgtg ccgcgatctg
     2401 aaggacagga tcggcagaag tatatacaat taaatcgcct gttttcattt ggtgttcacc
     2461 gtaatcgtca ataacagctg ttccgctata aggtttgtta catacgactt tacggccaga
     2521 aaattcttcg atttgtttga tcagatcatc agggaatccg ttcggaaaca cacgaaatgg
     2581 agttttgata ttcaatccca tgatttccca gtggcctgtc atcgtatctt tccctacaga
     2641 aacttcttct aatttcgtcg catagccttg atgatctttc actgattcca caccttcaag
     2701 aggacggatc gttcctaaac caagctgctc taaatgtgga acgttcaatc ctgcttcttt
     2761 cgcaatgtgt cctaatgtat cagaaccaac atcaccgaac ttatcggcat ctggtgcttc
     2821 tccaattcct actgaatcca tgacgatcag atgtacgcgt ttaaacatga ttattccttc
     2881 tttcttttta tttttattgc ctttaaaaca aaaagggcga gacgaaaaac acttcccgta
     2941 acccaccctt tttattcgct aaaagattat tttgatttga tatagttctt cccgtttgct
     3001 tttggaccag aagctttccc taagaaaaca accagcacaa tgattgtcag caaatatgga
     3061 gcaatttgca gcaatactgg cggaatcgaa gaaataactg gtaattgtgc gccaatgata
     3121 ctcaagcttt gagcaaaacc aaagaacaat gaggcaccca ttgcccctaa aggattccac
     3181 ttaccaaaga tcatagcagc catggagatg aacccttgtc caacgatcgt accagctgaa
     3241 aagttaccgg aaatcgactg agcaaaaaca gcaccgccca taccgcctaa caagccagaa
     3301 atcagtacgc ctgaataacg catagcatat acattgatac ctaatgtgtc ggcagcttga
     3361 ggattttcac caactgaacg gaggcgcaag ccgaatttcg tcttgaagat gatcacccaa
     3421 gctaggacag caatcaaaat agcacaataa gcaggtaaga atgtattttt aaagaaaatt
     3481 ggtccaatga ccggaatatc tgctaatcct gggaaagaaa aataccccag attctgcgtg
     3541 atgttatcag tttgaccttt accataccat gctttgatca agaaaacacc taaagcaggt
     3601 gccatcagat taattactgt cccgctgata atatggtcag cacgcaaact gactgttgct
     3661 actgcgtgaa tcagagaaaa taggatacct acgacgccac ccactaaaat agcgatccat
     3721 ggtgtcatag aaccaagtga tggactcatc gtcaagttga aaacgatgga actaaaagca
     3781 cccatgacca tgattccctc tagccctacg tttacgatcc cgcttcgttc agaaaaagtt
     3841 ccgcctagtg acgttagaat tagcggtgtt gaataaacca gtgtagaagt aaggatcaag
     3901 gcaattaatt cgatagtcga cattatagtt cgcccccttc atggttttga ttcgttggtt
     3961 tcgattctat tgtcgcaacg atctcttctt gttttttccc ttggaagact ttagccatga
     4021 cgaaacgaat caggaagttg atcccgacaa agaagataat caaagcaatc gatacattga
     4081 cgatctcaaa cggaacacct gctcctgtct gcatgccaag tccaccgatc ttcaaaatac
     4141 tgaacaagat cgctgataaa agaataccga ttgctgatcc agaaccaagc aaggaaacag
     4201 ccattccgtc aaatccaata gatagcgaag tcgtttgaac aaagaagttt tgatacgttc
     4261 ctaatccttc aaccacaccg ccaagccctg ccaaagcgcc agaaatgacc atcgacaaaa
     4321 cgatcgtacg tttactactc attcctgcat actcagaagc aaaaggattc aatccaacag
     4381 aacggatttc aaatcctaaa gtcgttttct tcatcagcag ccaaacgaaa ataagcgcga
     4441 tgattgctaa aaataatcct gagtttacgc gagagccgcc aaaagcttgt gttaaccatg
     4501 gtaaccgcag tgaggcattt tgactgatca tattggaaga gtccaaactt gagcgatagc
     4561 tttcaggcat cacttcatgt aaagcaaatg tgcttacgta taataagata tagttcatca
     4621 tgattgttac gatcacttca cttgttccaa aaaatgctcg aagtaaacct ggaacagccg
     4681 ctgccatcgc accagctaag gcacctaaga tcacgcataa cggaagcaaa acaacttttg
     4741 gcagatctgg aaacgcaagt gccacccaga cactgacgat ccacccgcaa agtgcttgtc
     4801 cagaaagccc aatattgaaa aacccagctg agttagctac agaaaaacct aatgctgtaa
     4861 aaatcaacgg cgttgcttgc cttaacgttt cacctatccc tctttgcgaa ccaagggctg
     4921 catctagcat agaagaataa ccttggatgg gctgaaaacc aaaagcagcc atcaatatcg
     4981 cgcctaagat caatcctaaa acaactgaaa gtacaggcac taataaatta cggaattttt
     5041 cgctgcgatc attcatgtgc gccagccccc ttttctaact cttttcgtgc ttcttctaag
     5101 gtatagcccg ccatcaatag acctaattca ttttcagatg tttctttcgg atcaacgata
     5161 ccgacaattt ctccttcatg gataacagca atacgatccg aaacatttaa aatttcttct
     5221 aattcgaaac tgatcagtaa cactgctttg tatttgtccc tttgttcgat cagacgttta
     5281 tgaataaatt cgatcgcacc tacatccagt ccacgagttg gatttgcaac aatcaacaga
     5341 tcaggatctc ggtcgacttc acgagcgata attgcttttt gctgatttcc gcctgataaa
     5401 gcttttgcgg gaacaagttc attcgttgtt cgaacatcat attcttcgat caattctctg
     5461 gcatgttcat tcattactga ataattcagc atgccgtttt tactgtaagg tttttgatga
     5521 taggtttgta gtgcgatatt ttcagataat gtcatatcta ggactaaccc gtatttatga
     5581 cggtcttctg gcacgtgtcc tacaccatgt tcagtgattt ttcggggttt tttattagtg
     5641 atgtcttccc cttttagctt gatatgtccg ctttctgcct ttcgcaaacc agtcaaagct
     5701 tggatcaact ccgactgccc gtttccatcg attccagcga taccaagtac ttcgccagca
     5761 cgaacctcta agttcaagtt tttcacggct tctaatccac gattttcttt caccactaga
     5821 ttttcaatag acaaaacgac ttcttgaggc ttcgcttctt ttttcatcgt tttgaatgaa
     5881 accgttcttc caaccatcat atcggctaat tgttgtgagg ttacgtcttt aacgttgact
     5941 gtaccgattc cttttccacg gcggataact gtacagcggt cagctactgc tttgatttca
     6001 tctaacttat gcgtaatcaa aatgatcgac ttgccttctt tgactaattc cttcatgatc
     6061 acgattaatt catcaatttc ttgaggggtc aatacagctg tcggctcatc aaaaatcagt
     6121 acatctgctc ctcggtaaag tgtttttaaa atttctaccc gttgttccat tccaactgaa
     6181 atatcacgaa cgtaagcatc tgggttgact gacaatccat actgttcaga aactttttgg
     6241 atctcttttc gtgctttttt atggtcaagc atccctgcgc gactaggctc gcttcccaac
     6301 acgatgtttt ctgttacagt aaaggcatca acaagcataa agtgctgatg gaccatccca
     6361 atccctaatt ggttggcttt ggtcggactt gtgatcttta cttctttccc acgcatcaaa
     6421 attttccctg atgtcggctc taataatccg gaaagcacgt tcatcaatgt agatttgcca
     6481 gcaccgtttt ctccaagcaa cgcatgaatt tctcctgcct ttacttgtaa attgatgttg
     6541 tcatttgcct tgaatgttcc aaattctttg gtaatattct gcatttctat gacaatattt
     6601 tcttgaacca cctttttttc acatcctaac gatttctatc tagggcaacg attgactaag
     6661 attcacgctt agtgagcgtt cttcttttga aacaaacatt acttcaggga gttgttccct
     6721 tatccaaact gtcacaaggc agcttggata aatgaacaat tcttgttcta atcaaaccat
     6781 agccttgtca aacatgtgtc taacaaggct atagaaatgg ttttagtttt cttcaggagt
     6841 ttctggaact tttacgtcac ctgaaatgat tttttctttt gcttcatcaa ctgctttttt
     6901 cgcttcgtca gaaagttgtc cttctgttag accaacaccg tcttctttca atccataaac
     6961 agtgtgttcg ccgccaggga atttaccgtc cgctgatttt tgtgccaaat cttctactac
     7021 tgttccaact gctttcagtg tagatgtcaa tgtgaagttt ttcttttcgc cgtttaatgt
     7081 gtattcgcct tcgtctgatt ggtcgcggtc aacaccgata acccaaactt ttttgtcgcc
     7141 tgattcgttc aatgattttg cttcttggaa tacaccgtta cctgtaccgc ctgaagcatg
     7201 gaagatgata tctgcgtttt gtgcatacat accttgagca attgaacgtc ctttatctgg
     7261 agcagagaag tcaccagcat attgattcaa tactttgatt tcttttccta attctttcgc
     7321 acctgcatca acaccggctt tgaatccagc atcaaaacga tcgattactt caccttttac
     7381 accgccgata aagccgacta cgtttgtttc agtcgtataa gctgctgcaa cacctgcaag
     7441 ataagaagct tcgttgtctt tgaaagttgc agatacaacg ttatctaatc catcgatgac
     7501 atcatcgata ataacaaagt tcgtacctgt attgtttgtt gcttgttctt ggatcgctgg
     7561 ttttagttta taaccaatac caaaaatcgt tttgaatcca gcattcaatg cttggtcaat
     7621 atttgggatg taatctgatt catttgaaga ttggaaatat tggaagccat catttcctcg
     7681 tgaaagtcct tgatctttcc cccatttttc tagtccttcc caagctgact ggttgaatga
     7741 acggtcgtct acaccacctg tgtctgtgat caatgctgca gttgttgtgt ctgatgagct
     7801 tttgccaccg gatgaatcag tagagccgtt gttgccgcat gcacctaaaa ataaccctgc
     7861 tgctagtgcg acagcaccta aaccaaataa ttttgctttt ttcatttctg taaagcctcc
     7921 taaaataatt gttttatttt tcaacagtca gctgactgaa tgaacatgag ttatagatct
     7981 ttttcagtaa atgcataagg cagcagtgca cgaacggtcg ttgccttcgt cacaccattg
     8041 tctcctacta gcgtaaccgg catatcaggg gaacagaatt ctgccattac ttgtcgacat
     8101 gcaccacaag gagagatagg atcgggggtg tgccccgcta cgactagatg cgtaaatgtt
     8161 ttttctcctt cagatactgc tttgaaaaaa gctgtgcgct cggcacaatt cgttaatcca
     8221 aatgatgcat tctcaatgtt gatgccttga taaatcttgc ctgattccgt tacgagacaa
     8281 gcgcctaccg gaaaatggga ataaggaaca taggctttat ctaacgcttc aatagctgtg
     8341 ttgatccatt cttgtttgag ttctgccatg agcttctcct ttgaaaaaat taatagcctt
     8401 caccagttga accagatacg attgctactc ctgcgcttgt tccgattctt gtagcaccag
     8461 cttcgatcat tgccattgct tcttcttcgt tatggatacc gccggaagct ttgacaccca
     8521 tttctggtcc tacagtttca cgcatcaaac ggacatcctc tacctttgct ccgcctgttg
     8581 agaaaccagt tgatgttttg acaaaatcag cgcctgcagc ttttgctatt tcgcaagctc
     8641 ggatgatttc ttctctatct aataacgatg tttcaataat aactttgaca agcgcacgat
     8701 cttttgctgc ttcaacaact gcttcgatat ctttttggac ttgttgatca tcctttgatt
     8761 tcaaagcacc gatattgatg accatatcga cttcatctgc accgttattg atggcatctg
     8821 ttgtttcaaa tgcttttgtc tcgggtgtgt ttgctcctag aggaaacccg attactgtac
     8881 atacggctac tggctcacct tgtaattgtt ctttcgctag cgcaacccat gctggattga
     8941 tgcagacaga ataaaaatgg tatttttttg cttcttcgat aatttgcatc acagcttctt
     9001 tagatgcgtc tgctttcaaa atagtatggt cgatcattcg gtttagttcc atttcttttc
     9061 cccttttctt taaaacggta aagtccgctt ttttccttta ttctgtgatg atgtcatgaa
     9121 tcaacgttgg cttcattgcc gattcaccaa tttcaatatt tttatataat aattcaatga
     9181 cagaagtgat ttcttcgtcg ttactataga tcgtcaatag tgactcacct tttgtcacag
     9241 tgtcaccaat ttttttatgc agtttcaatc caacagcgtg atcaatatct tcttctttgg
     9301 ttttacgccc agcacctagc atcattgcag caattcctaa ttcatttgcc acgatttttg
     9361 acacaactcc gctttgttta gcaggaagct ccattgtgta gcgtgcagtc aaaattcgtt
     9421 ctggatgttc cacgatcgct gggtctcccc cttgattttg gatcatttct ttgaattttg
     9481 ctaacgcttt acctgattcc agagcttctt gaagcaacgt tcttgcttca tcggttgtct
     9541 tagctttgcc agcaagaaca accatttggc ttcctaaggc gtagcacatt tctactagat
     9601 cttctggacc atttccttgt aatgtttcga ttgcctcgac aacttctaga ctatttccga
     9661 tagcttcgcc aagaggttct gacatatctg agataaccgc cactgtttct cggttagcca
     9721 gtttcccaat gctcgtcatg gtctttgcca gacgtcttgc atcttcgatg ttcttcatga
     9781 aagcaccgtc tccagtggtc acatccaaaa cgattgcatc tgctcctgcc gcgatttttt
     9841 tactcataat cgaactggca atcagcggaa gagaatcaac ggtcgctgtg acgtcacgca
     9901 aggcatacaa ttttttgtcc gccggtgcca agtttcctga ttgaccaatt accgctactt
     9961 tactttcatt aacaatgcgt acaaattctt catccgaaag ctcgatctga aatccgggaa
    10021 ttgcttctag cttgtctagt gtaccacctg tgtaacctag accgcgtcca gacattttag
    10081 ccactgtcac tccgacacta gctactaggg gggctaaaat caatgtggtc gtgtcaccaa
    10141 cgccacctgt cgaatgctta tctaccttta ttccttcaag agaactcaaa tcgatgacat
    10201 caccagaatt agcaatcgcc aatgttaagt tcgtgatttc ttcatttgtc atgtcttgat
    10261 aaaaaattgc catcaataat gcactgactt gataatctgg aatctttcca ttcgtatagt
    10321 tcgtgacgat gtactcgatt tcctctttcg acagctcgtt tccatccctc tttttttcaa
    10381 tcagatccac cattcgcatt gcctgatcct ccttgtcttc attcggtatt atacattatt
    10441 tttaggaaag gtaaaccact ttagtaaact ggtttactaa aaataaaaaa agcactaact
    10501 agcctttgta aatgcttaca taagcaatat accgccgagt tagccctttg tcaatacttt
    10561 tacgctctca cgaataatta attttgtttc gaacactttg ttcggaactt ttctttgagg
    10621 aaattcgatc gtatcgatca ggaaacgtgc agcataaaat ccaatatcaa aacttggctg
    10681 ctgcactgtc gtcaattgcg gaatcatgta ttctgacagc tttaatccat cgaatcctat
    10741 gactgagata tcttctggga tttttttgcc caattcatcg atcgcctgat agcatccgat
    10801 cgccatggca tcattcccgc aaaaaatagc cgtgatatct ttatgtttga gtaatttttt
    10861 tgatgcagta tatccacctt ggacagtcaa ctctccatgt tcaacaaatt tcccattaaa
    10921 aggcaggcca tgatctttca gcgcatgacg atatccgttg aaacgttctt ctaattgata
    10981 gtatccagtg gtttccttca gcatgccgat cttcgtatgg ccattttcaa taaacagcga
    11041 aacagcttga taggcgccct catattcctt tacgataagc cgtccgttat cccgacgatt
    11101 caatccgcga tcgattaaaa tgattggcat tttttgataa aaaccgctgt ctaatgcata
    11161 tttctcaggt aaaatatttg gtgtggccag caagattccg tccactgagc ggtgggaaag
    11221 ctcttgtaaa tacttgatct cattttcttg gctatgacga gaattgcata acatgatcac
    11281 atagcccaaa ggattcaaat agctctctac accttcgatc agtttggaga agaagaaatc
    11341 agtgacatca gggacgatca ttccgatggt ctttgaatgt cgattgatca catttgaagc
    11401 gaaataatcg ggcttataat gatgttcatt cacgatcgac aacacttttt ttcttgtctt
    11461 atcactaaat ctgcttcctt tgttattcaa aatctgtgag acagttgtta ctgatacgcc
    11521 tgccatttct gcgatatctc tgatggtcag cttcatccta ttggctccct tctatctaaa
    11581 ttccctatca gtgtaccaaa acccccaaga aaagaataga gaaaaaagga aagtatgttt
    11641 tttgataaaa acctccttta tctaccgact tggcagcctt ctatacaagt tctgtatctt
    11701 atttatttta tcgtaaaaaa caacgactct gttcctacta tttctttttg aagaaaaaat
    11761 agttctttgt ttttaaaaac ttctcaacaa aaaaagaact gatacgtacc cgtacatgca
    11821 cgcccagttc cactctttcg tcgctccact atttttcagc tagttattct ttcacacttt
    11881 tgatgatata atatccttta tcttttgtga cgatttctgc attaccaaat gtttcttcca
    11941 ttttcttttg cgcactcggc gctccttgtt tcttctggat cacaattgtc agtgtaccgc
    12001 ccttcttcaa ccgaggatat gcgcctgtca aaatttcatg tacaactttt tttccagcac
    12061 ggatcggcgg attgcttacg atagctgcat aggtttcttc atgaactgct tcatagatat
    12121 tcgattgatg gatatcaaca tgttcgattt ggttacgctt tgcgtttcct tgcgctagac
    12181 ttaccgctcg ttcattaatg tcgaccattt caacaaaccg ctgtgtcgca aatgccaaag
    12241 ctaacccaat cggtccatac ccgcatccca catctaagat ctttccttca tcaggtaact
    12301 cttcccaatc gaaagcatca atcaggacac gtgaaccata atccactgtt tcacgtgaaa
    12361 atacaccaga atctgttaca aattgaaagg ttctgccttt taactcaaaa gcccattgtt
    12421 caaaatcgtg cgccgtatct gggcgttcag aatagtaatg atttgtcatg tcttccatcc
    12481 ttctcatttc tttcatgaca acagtctatc cttttatgta tccgtgcgca agtcaagaat
    12541 aagactccgt ttctttctat tataaagatt atacaaaaag atcggcaatc tctcttaagt
    12601 ggtggggttt gcgtttattt tgtgttaaaa tagttccatt gatgggagaa cgcaggaggc
    12661 taatatgaat gatccaatga actactaccg tgtggcaaga gaagaatggc gagaatttta
    12721 tcgtaatggg caagcacctt tgacacaaga tgaactagat aatatcaaaa gtttgaatga
    12781 ccgcatttcc atgcaggatg ttcgagatat ctatgtacct cttgctcatc tgatttatct
    12841 ttatatgaaa gaatttgaat ctttgttctt gagtaaaggt ttatttcttc ataaatacgt
    12901 agaagtaccg ccttttatta tcgggatcgc cggaagtgta gccgttggaa aaagtacaac
    12961 agccagactg ctgcagacga ttttgtctcg taccttcaaa cgaagaaacg ttcagctgat
    13021 cacgactgac ggatttcttt atccaaatcg tatattaaaa gagcgtggaa tcatggaccg
    13081 taaaggattt cctgaaagtt atgatatgga gatgctgatc gatttcttga accgtgtaaa
    13141 atctggtcag gaagagataa aagtacctgt ttactcacat gatgtgtatg atatcattcc
    13201 agacaagtac gaattgatcc agcagcctga tatcttgatc gttgagggga tcaatacgct
    13261 tcaattacct gcgaatcagc aaatctacgt cagcgacttt tttgattttt ctgtctatgt
    13321 cgatgccgat cccgtgctga tcgaaaaatg gtatttggat cgttttggtg cgttactgga
    13381 tacggctttc caaaatccag agaattatta ttatcgtttt gctatcggag atcgtgaaga
    13441 agcctttaaa atggcaaaag atgtatggaa aaatgtgaat ttgaaaaatt tacaggaata
    13501 tatcttgcct acacgaggaa gagccgatat aattctccat aaaactgaaa atcatatcat
    13561 cgattcgatt ttcatgcgaa aatattagca ttttaaaata gtttgactat tttgagataa
    13621 aatttaaagg gataggggta ataaatttga caaacgttgc cgatatgacg aacgttgaaa
    13681 agattatcgt attagactac ggtagtcagt ataaccaatt gatcacaaga cgtattcgtg
    13741 atcttggagt cttttccgaa ttattgagcc accgcattac tgcagaagaa gtaaaagcga
    13801 tgaatccaaa gggaatcatt ctttctggcg gaccaaacag tgtatatgat gagaatgcat
    13861 ttgggatcga tcctgagatc ttcaatttag gtattcctgt attagggatt tgctatggta
    13921 tgcaattgat cacgtacaaa cttggcggtt ccgtagagcc tgctaaaaac cgcgaatatg
    13981 ggaaagcctt gttagaagta acaagcgatc aagctgtctt gtttaaagaa acaccaaaac
    14041 aacaaacagt atggatgagc catggtgact tggtaactaa agttcctgaa ggctttgaaa
    14101 cagtcggaac aagcaaagat tgtccgattg cttctatcca agacacaaca cgtaattttt
    14161 atggtatcca attccatgca gaagtacgtc attccgaata cggaatcgaa ttgctgcgcc
    14221 actttgcctt tgatgtttgt cactgtgaag gtaactggac aatggataac tttatcgata
    14281 tgcaagtaga aaaaatccgt gaacaagttg gtgacaaaaa agtcttgctt ggtctttcag
    14341 gtggcgttga ttctagtgtc gttggggttc ttttacaaaa agcgatcggt gaccaattga
    14401 catctatctt tgttgaccac ggtctgctac gcaaaggcga agcggaacaa gtaatggaaa
    14461 gtctaggcgg aaaattcggc ttgaacatca tcaaagtcga tgcaagagat cgcttcttag
    14521 acaagttgaa aggtgtatct gacccagaac aaaaacgtaa aatcattggg aatgaattcg
    14581 tatatgtttt tgatgacgaa gcaacaaaac ttgctggtga agaaggcgtc tctttcctag
    14641 cacaaggaac gttgtatact gacgttatcg aatctggtac agaaacagca cagacgatca
    14701 aatctcacca taatgtaggt ggactgccag aagatatgca atttgaattg atcgaaccat
    14761 taaacacatt attcaaagac gaagtccgtg aattagggac tcagttaggc atgcctgatt
    14821 caatcgtttg gcgtcagcca ttccctggtc ctggtcttgg tattcgtgtt cttggcgaaa
    14881 tcactgaaga caagctagaa atcgtacgcg aatctgatgc tatcttgcgt gaagaaatcg
    14941 cagctgctgg acttgaccgt gatatctggc aatatttcac tgttcttcca gggatccgct
    15001 ctgtcggtgt tatgggtgac ggacgtactt acgactacac agtcggtatc cgtgctgtaa
    15061 cttcaatcga cggaatgacc gctgactttg cccgtatccc atgggaagtc ttgcaaaaaa
    15121 tctctgtacg tatcgtaaac gaagtagccc acgtcaaccg tatcgtgtat gacatcactt
    15181 ctaaaccacc agcaacagtg gagtgggaat agttgtagga gagctagaaa tcctcttata
    15241 ccaagggttt ctggctcttc ttatcttttt actgcacttt tattgcactt ttttcatttc
    15301 tttgtttcgc aagtagtcta cagtaaattg attctttggt gaagtatcat aattttgttt
    15361 tgcttcttta aaggaaatgg ctccatttaa tttgttagct acctcgaaat tactgttagg
    15421 ataaagatgt ccatacgttc ctaaagtcgt ctcaatatct tcgtgtccta gacgatcttt
    15481 aatgataagt ggattttctc ccatactgat taacaaggaa gcatgagaat gccgtaaccc
    15541 atggattctg attcgatgga taccagccag cttagcatag cgttcaatag catatgatag
    15601 tgtatgtttt tgggtaggaa taccgttata gctcattaca aagtctgtct gaattagatt
    15661 ttgttgtact tccttccatt cggataaata agttaatgtg catttatcta atacaatatg
    15721 acgaacgctc gcctttgttt ttggttcaac aaatctataa ttcgattgat tcttgtaata
    15781 gagtgttttg ttgatggtta gtactcctga gtcaaaatca atatcttccc attgaatggc
    15841 agtagcttca ccaattcgca ttccagtcat aaataaaaac cacagagaga taaataaaaa
    15901 gtgttgatag taatcttctt tgtaaattag agagattact ttttcaaatt cttcttttgt
    15961 ccaaaattca attttagact tttgcttttt cacattgcca ataatcttag atggatttgt
    16021 ggtggttatc ccaaggacaa ttgctcgatc catagcaact gaaaatagtc cttgaacagc
    16081 tcttacataa gaagacttac acttctttga taattttagt tgccaatttt gcacatcaat
    16141 aggctcaata tcggaaacag ccattttatc aaaataagag aaatgcttct taatagttgg
    16201 taagcgattt tcatatgttc tgagctttac ctgtgtctta taccatggaa ggaaaatttc
    16261 aaagatataa tgtttaaacg tcattttatc cttattctca cttaattctt ctggtttcat
    16321 caatagcagt ttcgaatatt cttctcttgc ttctttttta gtagtgaatc cactacgata
    16381 tttttgaatt tttttccctt tagaatcata acctagattt gcacgaaaat aataggttcc
    16441 attctttgct ttttttattg ggtctttaac catagttttt actccttact gtgcaaaaac
    16501 tactcagacc tgttttcatt tgaaaaccga attcctagaa tttcttcaac agcttcacga
    16561 gggactctat ctagctttcg agattgatag tagatatgcc cttttgtaat catcaattct
    16621 tttgcttttt ttatgatgtc tgctgaaaaa gaagttccat atcctagctc aactaaatcc
    16681 ttttttgtga ccgttatcat tattactcct ttcccaatta taggctagga aaactccaat
    16741 attcacctat aattatatca aatagtttta taataataca ctgacaagag aagaatatct
    16801 tagcaatcga acagctctag caaagctcac aggaatttca cttctgcatg gttctcatgt
    16861 agccgtaccc attgcctgtg acgcttctaa cgctcggact atggctatac gggagtatca
    16921 ttatcacgat aagaaagtca tggcagcaat cctgctaaag attactttcg aattactaac
    16981 atgaatcgct cactaatttt gtaggtcatg gcgtgttagt tcatcagctc ttttcttacc
    17041 gaaacgtatt cgattgcctt tatttggttg tcaaagaacg tttggcttgt tagcctatca
    17101 aaacctactg aacacacaat atgctttgat ggaataacaa acctcccaaa tcgggaagcg
    17161 tggatacgat tcgacacgct tccacgaaaa aatgggacta tttaatttcg aacgataaaa
    17221 tcttagagat gagtttcgtt tccattctgc cacgtagggt ttcatcaata accatatgag
    17281 aatttccata ttcgtctgtc atgcgacgta atgaacgttt tgaggtatac cctcgataat
    17341 gtttaacaat ctgattaatt gcttccacgt cgccatctgt cgcctttaca atgaggggga
    17401 agggaaccct tggataagct gttttcattc tttacactcc ttcatgaatt ctttaatcat
    17461 ggctagtcca cttattcgat gacgataaac agtcgaacga ttcaaattta gtaactccgc
    17521 aatctccaca tcactcatat ccataaagta atgaagtaaa ataatgttcc gttttttctc
    17581 tgaaagatga cgcagggctt cacttaataa atcactttca acattgattc ttaatccaaa
    17641 taatttaaat agttggaaat cagtgacgta cgtatcaaca gtagaaaaag agttgacaag
    17701 gtagttatct atatcagaaa acgagacttc ttttgctgat agtctgtcta gatgtttgaa
    17761 gtaatctttt cgttcatctt caataacttg tttgcagata tagtcaaact gattttctat
    17821 ggtttcttga aaagaagatg gtttcatgct tttcaccccc tttctgtcgt gaaagggagt
    17881 ggcatgtgct tctttatctc ccttcatact aaaccccgac acgaacgggg gatttgttgc
    17941 ataaatgaag agaaatccta aaagatattt attaagtgac caaaaaagca cataaacaga
    18001 ttgttttctc tgttcatatg ctttgatttt tctatctatg taatctgcaa aaccacattg
    18061 atggtcatat ctatccagta caggtggata agtttttttg ataagaactt aattaagaga
    18121 actagatgac agattatctt tctcatggcg acttttccct ccattaagtc gccaatttta
    18181 tctactttct aggagtatga ctaaatttta taaagttgta taggtaaata aaatcagcga
    18241 cctttctatt tgaaattgga ttcatcgcat taataaattc cacagtgttc tataggaact
    18301 cataaactat ataacatgtt tttctatacc tgtttatggt tatataggaa cagtaaaatg
    18361 tatagaggtg atttagtatg cgtaaaaaag aagataaata cgattttaga gccgttggtt
    18421 tagcgattaa agaggctcga atgaaacggg gtctcactcg tgaacaagtg ggaacaatga
    18481 ttgaaattga cccacgttat ttaacaaata tagaaaacaa aggacaacac ccaagtacac
    18541 aagtgctgta tgatcttgta tcattactcc atgtatctat tgatgaattt ttcttaccta
    18601 cagataattt gattaaaagc actcgaaggt tacaggtaga aaaatacatg gatagcttta
    18661 cagacaaaga actatcctta atggaagcac tagcaaaagg tatcaatgaa gcaagaaata
    18721 ttgaaggcta gtcagtaaaa ttcagataaa caaaaagagc cgataagatg agagtttcat
    18781 ttctcaaatt atcggctctg cgtcttggcg tctggctctt tgattgttat tatattcatt
    18841 ttttgaacat taattaaagt ttcgttctta catttggggc agtataaagg gaaattttta
    18901 agtatagtat ccactcgtat tcgtagtctt gttttatttc cacaaatagg acacaatatc
    18961 cacttgtagt ttataataac aatctcctcc tttccacttt aattcaaatc tatattaaag
    19021 aatatttcat cttatttaat aagaaaccat atttatataa caacataaaa cgcactaagt
    19081 tattttattg aacatatatc ttactttatc tatccgacta tttagacgac gggtctggca
    19141 aacaggttcg ccagtggtaa cctgatatcc ttttagctct gctaaacaaa cactaagccc
    19201 atttgtaaaa aaagttaaat cattgcgata atcttgaata catcgagcag gaatttctcc
    19261 aataataatg acctcattat ttttcagttg agtatttacg atatttgcac aatatttggg
    19321 agcatcgtta tatgcccgtg aaagatattc ctgtggtgca taaactttaa aactaagata
    19381 tggctctaac aattctgttc cagcttttct aaaggcttgc tccagtacaa taggagtaag
    19441 catccgaaaa tctgctggag tactaacagg gctatagtat aaaccgtact taaaacagat
    19501 tttacaatcc gtcacattcc aaccatataa tccttgttcg caaccatagc gtatcccttc
    19561 cataactgca ttttgaaatg attgatttaa gtatccaaga gaaaccgagc tctcatactg
    19621 cattccactt cccaacggaa gcggtgatac agataaacca atggaagccc agaaaggatt
    19681 tggcggcact tcgatgtgaa tggtatattc tgcatttttt aacggtctct ccatataaat
    19741 gactgtaggc tcttttagtt ctatctccac atgatacttt tcttgcaaca gtgcactaat
    19801 cacttccatt tgtactttcc ctaagaaaga aagtataatt tcatgtgtcg tagaatccac
    19861 gtaatatcgt agaagcggat cactatctga gatttccaaa agggcatcaa gcaacatttc
    19921 tctctgttca ggtttactcg gttcaacagt tgtttgtagt agagggtgcg gattttcaat
    19981 cttttttctc tgtggcaata gttttgtatc tccaagaaca ctatttaact tcaaaaactc
    20041 attttgcaaa ataacaattt ctccagaata agctctatca atcttacata attcaccatt
    20101 tattgaagta tacatttctg taacttttat tttttctttt tctgatactc taaccgaatc
    20161 tcgtaaatgt agtactccac tataaaggcg tatatatgca agtaagtagt tcagttttgg
    20221 agtacaaaat gacaaaatgt acttcaaaat ggactacttt tttcatttta acttacatac
    20281 caaattacat acaaaatgat ttataaaatg caattgaaaa tgataattga atgaaaattt
    20341 gtgattttgt ggtattatga taatataact tatagaaaaa attgcttatt caactaacgg
    20401 gcaggataat gagaaaaggt tttttgatag aaataagaaa ttagatggta aattagttaa
    20461 aaaggctgtg ttataaatga aagtttcttt gattgctgcg atggataaga atagagtgat
    20521 tggcaaagag aatgacattc cttggaggat tcccaaggac tgggaatatg ttaaaaatac
    20581 tacaaaggga catccgataa tattaggtag gaagaacctt gaatcaatcg gaagagcctt
    20641 acctgacaga agaaatatta ttctgacgag agataagggg tttaccttta atggttgtga
    20701 aattgttcat tcaatagaag atgtttttga gttatgtaaa aacgaagaag aaatttttat
    20761 tttcggagga gaacagattt ataatttgtt tttcccttat gttgagaaaa tgtacatcac
    20821 aaaaatacat catgaattcg aaggagatac tttttttcca gaagtgaatt atgaggaatg
    20881 gaatgaggta tttgcccaaa aagggataaa gaatgataaa aatccgtata actactattt
    20941 tcatgtatat gaaagaaaaa acttattgag ttaacgggtg cgttaattga agaagaacag
    21001 taaagataaa gcacaagagc ttgggattac tccaagctct ttttgtcatt tgaaataaat
    21061 tatatctttc caatcaaatt gtttatctgt aatccctaat ctttgagcag gtgtcaaaac
    21121 ctttttatcc gctgatttaa aagggcgaca aaagttgtag taagttctta gaattgtaat
    21181 cgcaaattga gcatacttcg gattgaagtt agcgtaaata tagctcttct tatcaccacg
    21241 agctgttgtt aatggtcttt caagagatga tatataacgt cttatctgtt gaataaagct
    21301 attggtcgaa tggtcattaa catttaatag catctttgcg atttcgtttg gttccagagc
    21361 agataaatcc gttctacaat caactgaata aaatccttta tccttagtgg ctaatggatg
    21421 tttgataggg ttatcggcat atttgcgata tctttctcca tctttactaa tagcctcttc
    21481 gtggaattga tgacttgtta gtaattcagt tagataatga gatgccaaag tccgtagaaa
    21541 ctttgttttg aatcctctga tatctcccca atcgagcagt tctgcttttg cttgtccgaa
    21601 ttcttttaag cattgttttc tgcttttcgt cttgttaact tggcaaataa aatgatgtgc
    21661 atcgctcaac cttaattctt tcgtgaatac tctataaaag gcgtttctaa tagagaagtc
    21721 atcatcacta atcattcgcc attcgtcggc attaaccatt tgtttaatta accaatagtg
    21781 agcagttgta gtataaggag catcaacatt taaaccatcg acaaatctac tcctattagt
    21841 aattttccct aattcgtgaa gataagcatt cctattttcg ctatcgttag cggatggttc
    21901 ttgtggataa taagaccaat ctaacctatc atttttacga ctaaaagtat tgagatggtc
    21961 ttctttgaat tttcttgtat cttcatttaa ttcatccatt gaaatattcc aatcataggc
    22021 aacatcagaa cggaacacat atcttgataa gacctcagca gatacaacta tatacgtttg
    22081 catattaagg tcttcaaacc ccgtatattt ctttgaacct tgtcctttct tacggacgtt
    22141 atttaggtag tagtgcatct tgtccgtatt aagccacatc tcgttaaatt tctttgtttg
    22201 aaggggttgt gtctcatatc gttctaaaaa ctccaaacaa cgcctataca gccactccag
    22261 cttatgatag taggttccaa ctccaattcc taaaatatca caagtacgac tgactgaaac
    22321 ccttccaaca accatcttag cgaacattgg aagaatggtg ttcttttgtt ggtgataagt
    22381 agtggtttct tctctttttg gtagaacatt agtgaacttc ttacacgcct tacattgata
    22441 gcgttgagat ttgcccctac ttttccccct tttgtaaaat tgcttcggct cattgaatgg
    22501 ggttgattct tcttccgagc agccttcttt atgaaattga taatctggct caacatcttg
    22561 gatgctattg atttctataa ggcgtttaat ttcttcaaca acagaccaat tagataaagg
    22621 ggttactgta cagttttgcg atacacctct gccaatagga ttgtcattgc agtacatacc
    22681 tttatgacct ttatctttgg aactaccagt cattttatat cgacttggtt tcccttttac
    22741 atctttgaat ttgtgctgtt ctttcccaaa attacaacaa aacggattga tgcagtaatt
    22801 atattgaatg ggatattcag aaccgttcca tttaaaagac gttggcaaga acaacatttt
    22861 gttatacctt gttgcaaatc gttttggagt taataaatca tattgtttag ccctatcttt
    22921 taattcttcg tttgaaatcg gaattttaac atctacaatt tcatcttcct tcgtggctaa
    22981 tcttttaagc ttggtcattg tctatcaacc cactctatct ttgataccca ttttttcaac
    23041 aatccgctta ttacttcgct tgttggcaat ccattcaata aacttcttat cgtctaaaat
    23101 gttcaataag aattcatcca ctgcttcgct ttcagtacgc tctgcatatt cagcgtattg
    23161 cttaactatc acccttactt gctctgatat ttcccaatcc acagacttag cgttcttatt
    23221 ttttggctca agaaattgca taggaaaatg cacccctttt tgcttagtaa attgtaacca
    23281 ttttaacaca ccaatttgca ttcctacaat aattatttca cgttcgcatt tttatataca
    23341 ttttaactta cattttgtgg tgtaaattaa caacaaacgt tgatttaaca ataaaaaacg
    23401 agtatcaaat tcagttgcaa tttgatactc atttaggttt acattttcac tttttgtact
    23461 ccaaaactga actacttaat atgcaagacg ttgtcttttt tttgtatatt caattttgaa
    23521 aacatttccg caaagttcag acggacctcg atgtgttgat gaataaaatt tattagtaat
    23581 aacttctata aggttatcaa tccctatatt actttttgca cttccatgat aaagagggaa
    23641 cagagaacaa ttctgaaatc ttatgctttc ctcttgttcg agttccaatg cttctaatga
    23701 tttaccggac atatatttct ctaaaaggtc atcgtttccc tctattaccg tatcccattg
    23761 ttcagattcg gtaaagttcg tcacacacat attaggatac agttctacct tctgtttgat
    23821 tacaatttcg gcagaaagtt tctctttaat atcctgataa accgttgata aatcaattcc
    23881 attttggtca atcttattga taaaaaagat tgtgggaatc cccattttcc taagtgcatg
    23941 aaataatata cgagtttgtg cttgtacgcc atcttttgca gaaatcagta gaattgcccc
    24001 atctaaaact gataatgaac gatatacttc tgctaagaaa tccatatgtc ctggcgtgtc
    24061 tatgatgttc accttcgtat tttcccactg aaaagaggtt attcctgtct gaattgtaat
    24121 tcctctctga cgttctaaaa gcgtattatc cgtcctcgtt gtacctttgt ccacgcttcc
    24181 taattctgta atcgctccac tgttatataa taagctttct gttaaggtag tttttcctgc
    24241 atcaacatga gctaaaactc caatattaat aattttcatg tgattttcct ccattcaaaa
    24301 acccaaaagg gcataaaaat cccagtgata aatactttta tcactgggat ttttatgcat
    24361 aaccataggt atacaaagca tacagatatt ctctggatac tttagaatca catgataaag
    24421 gtattcttaa actgggtaca aaaaactaag ccctcctaaa aaaggacatc taattatttg
    24481 ttcccgctat caaattgaca gtttatttaa gaataccttg ccgcatattt attaactcct
    24541 tttaaatagt cacttaaata atagcacgta agagcatatt tgtaaaggaa tctccaattt
    24601 tttatcaaaa agagtacatg attacaaagt atctgtaatc atgtaccaat atttgttatt
    24661 ttacaatctt ccaattatct tgtttttcta atatcaactc aaattgcgag atttgggttg
    24721 cctttgtttc ttgatctaaa tatttcacag cgacattcac gattacttgg ttatcttttc
    24781 tggtaaagat aggattcacc aattcctcga aaacatattc cttgttaatc ataggtaaca
    24841 catgattact tacatagtaa gtcaattctt tttctgtggc ggtaggatag agctggaaga
    24901 acgtttctaa aaagctagtg atttcgtcca tcgtttctgt atctactgta tggtcacttt
    24961 ctagctgttt gggttggtag tccgattttt gaggtttctt actcattgtc ggatttttga
    25021 taataaccat attatctgat tcatctatat ggacaaccac atgaaagcta gacgatatag
    25081 tttccttgtc tttgtcttcg gtaataactt gttcaacaga aaacagtacc tcaaaggtgt
    25141 tctcattcac ttgagaaacc tgccacacct gtatatcgtt cacactagaa ctagtaggaa
    25201 tatccttacg aatcatttct tcatttaata cctgcaattc ttccgtcaaa taatgggtca
    25261 atttttcatt tcgtttatca atggcttctt gggattgttg ccacgaaaag tagtctttcg
    25321 caaaggattt cacaaaactt tctacttggt tggtatcgac aatctgctgt ttaatgattt
    25381 ctgtttcacg gacagtatga gtgtcaatgg cagtaaaatt tttatagatt ccaaatccga
    25441 cactaccaat caataaaacc cataaaacaa aggttaattt cttatgcgtg ccaactttaa
    25501 tcatcggaac ttttctttgc ttgctcggtt ttttagattt ctttggtttc tccttctttt
    25561 cttttcgctc gatttttata atcatttgat tcattccttt cttagttatg aataacacgt
    25621 ctagccccaa tcagatggtc ttgccaataa cgactgctta agtcgccata accgattggg
    25681 tcgcctgcat gataaaagcg atttccctct aaatatatcg ccacatgagt cacatatgtg
    25741 cctgcattat aggttgaatg aaagaagata aggtcgcctg cttgggcttc ttccatagag
    25801 atttcttgcg tagcgtcata ctgatcttgg gcgacacgag ggagagaaat gccagctttg
    25861 tcatacaccc attgtattaa gccactacaa tcaaaagagg tggtaggcga cgcccctcca
    25921 aagacatagg gaaacccttc gtatttcaag gcttcgtcca taatgacttg cacggtttca
    25981 tcatcaaatt ctgttgggga agtgtcggtt aggtattgtg aaaccaactg aacataaaac
    26041 tgatttccat agttatatcg ccaaccacca ttcacaggta tggctatagg attgggatag
    26101 tctgcttttt gaccacccga cttctcttta gaaaactgtt cggctaattc ataggtatat
    26161 tttttcccat gactcctcac ataatttaaa aagccaccgc cataattata agattggata
    26221 acagaatcta tatccactcc ttgttgttct gcactggtta ataattcact gaaatattta
    26281 accccttgtt tgatggactc ttcggtactc aaactgttgg gcggtaaacc caaagattcg
    26341 gaactttgca taacatcttc tgctgtaccg cccgattcca cttgcataat ggctaagata
    26401 taagaaacat agtcttcaat cccatattcc ttaccatatt tttcaatcaa cggacggtgt
    26461 gccaacactt ctggtgaaac ggtcacaccg ccttgtggaa tagaagaatc cttgttgttt
    26521 tcgccactgt ccgtatcatc tgcaaagaaa atgactagga ataataagag aaaaaagaag
    26581 agagagaaga ccacactaca aatcactgtt atttttagct tcatgtttgt tgtcctcgct
    26641 tatccgttct tttatttagc ttcctctgat tgaattgctt cggtggtgtc ttttgttttt
    26701 gctctttttt caatggtcgc tgatgatttt cacgtttagt gttttgtgac aaaacaggcg
    26761 tgacggtttg ctgacgcttg cgaacatgct gctggttatt ctgaacagga gatgatgggt
    26821 tttcttttcg tcttttttct tttgctttat ccagctccaa acgcttttga gcaatgttct
    26881 ttcgtttttg ctcctgctgg ttcatacgct gttgtttgcg ttgagctttg gtatctgcca
    26941 tagattgttt aaagtctgtt acattatgag tgacattctc ttttccttga tgaagggcat
    27001 atttgacatg ggttggtgtc gcctgtatct gttgttttgc ctgctttctt ttattgacta
    27061 gacgattttt cgtgtccaat acatgggctg tcttttcacc taatcgttgc ccgataccgc
    27121 cttgttgctt tttcacattg gaaagtgctg tttctttctt tggtgtagtc cgttcatttt
    27181 gacgagtatg gttagcttta ttggtttgat tctgctttgt tgtcattgtc ttttgcttcg
    27241 ttgaccctgc ttttccacga aaaatcttac tcatactgcg ttgaaatctt ctggtttgtc
    27301 gattcatcat ttgacgaggt ctgcgaaaca cttgtctgcc catgttttga gagtcgttgc
    27361 tttgtaaagt gaataggctc attaaatcgc ccagtttaaa ataaatccct gcaaaggtca
    27421 caatttgtaa aaacataatc atgaaaaagg gagaacttgc tgacagcgaa taaagcatag
    27481 tggaaatact aaaggcaacg gtaataatca aggtaatacc agcccttgtc ataatcacat
    27541 taaataactt ggtaatggca tgcctgccca tgccatcaaa actcggcaac atacttaata
    27601 ggaaactgat tggcaaaaac atagcgaaaa taataaatag cacttgcgaa aaaatcatga
    27661 ttcctgttag aaggaaaaca aaaatcgaga taccaatatt gaatacaaac aaaaagaaga
    27721 ctgttcctaa tctggaaatc gtcttcggta gggttaaata ggtattgtca tgatcttcaa
    27781 tttcttcaat cactacattt tctctatctt caccattgtt tgtatctgga ctggtagaca
    27841 ataaactttc cacacgttct tcccctaacg cttctacatc agtggagcca tattgaagca
    27901 atagccatgg ttgctttact tgaacagaga ataagctgtc acgtatcatg tccacactgt
    27961 ctttcccttg actatccgtg ttaggaagaa caatttttgt cccaatattc agactggcgg
    28021 tactgacatc tttggaaaaa tcattgattt tcgcaatata atcgggtgca tacgcaataa
    28081 aggaagccga tagaataaac accacgacaa agtttaaaac agctcgtatc actttcgtcg
    28141 tttctcgctt aaccaatcct gtataggcaa catagattcc aacaatgaga ataaaaagaa
    28201 ggaggaaacc gacataaaac cctgaactgg aaaagccatt cggtgtaatg cctgcaatcg
    28261 tctgcatgtt ttcgccaatt gcttgggatg tttgactaat gaaatctaag gcataggctt
    28321 ctttcaccaa ataacctgtg gcgttcgata gataaagaga aattgtccaa ataaaattag
    28381 taatggcata caatccatac atcaccgatt gtcctatgcc atcactccaa ttccatggaa
    28441 gccagcccca gctattgtcc acataataat caagctgata ttgattcaaa ccatacttgg
    28501 agtattcatt gcttgtatca atggtttcat caactaagcc tgtcgcttgt actgcattgc
    28561 ccagcgtgcc taataaaagt aaaataacgc ccacacaaag gagcgttatc caactatagt
    28621 gaagaaattt cttttgtttt tgtgtcattc ttccacctca tttcgcacag gcggtctggt
    28681 atcaaaggca tgaagcaact cttcaaaaat cgggtgaaac tgcataaccc ctacacgacc
    28741 atagatgtct gaaactaaac attgtccatt ttctaattca cgtaagcgtt tctgattgtt
    28801 ttcatcttcc ttgtccacac caaaaaattc taaggtcttt ttgatttcca ctaggtcagt
    28861 ggaacgaaag gcaaatttca agccaatatt attcttcatc ttttcatcta gtaaatcgtc
    28921 cgcgttttga gtaacgaaat agacccctgc attcatggca cgtcctgctc gaaccaatcg
    28981 catagataga gcttttcctt gtgccacttg taaaaaactc caagcctcat caaggtcaac
    29041 aattttaaaa atggaacggt cagagtggat aaagtctaaa gcaaaggtac taatgacaat
    29101 cagcattgcc acacttaata attccatggt agtgtattct tcaaaagagc tattggaatc
    29161 gggtaagacc aaatctgcca cttgaataat attgagttgt ttttccaagc taatggattg
    29221 ggtgacactg ccatcactga ataacagatg ggcaaaatca taatcgacaa agctttcgat
    29281 atggtcagca atactattgc tgatgggcgt atcttctttt cgcaattcat caatcaccaa
    29341 taacaaccct cgttgtttgc tttgagttac actccgaata gctttacgca ggacaggaaa
    29401 cttctcgcta tctcggctgg aaatacctgt taagaacgtg agaatatcaa tggcaaggct
    29461 ttctgaatct ttcgggagtt ttaaaatcac ataagggtca agtaaccctt tattgtcgtt
    29521 ctcactcgtg agattaacaa tattgatttc atgggcaata tcaggtaagg tttctttcca
    29581 ttcaccacgt tcagcttttg ggtcaacaat ggttgcctgt ccgccaaaga gtactgaata
    29641 atagaccagc atattgttag agaatgattt tccgccacct aatgaaccaa taaaagcgga
    29701 cgctaatgca tttgttacag agcctttaat tccttgactt gcaaggctag gttggagata
    29761 aatatttctg cctgtatcga gattgtaacc gacgtaaata ccagaatttt caccaatcat
    29821 ttgtgtagca ccgaaaccaa gaccagccaa aaaatccgac gtgacatatt gaatatagtc
    29881 attgatatat cgcttgcttg agggaataaa ttcattatgc aagcctagca tatcgccaaa
    29941 aggtcgtact aatttgatag acagatcatc ataaaaatct tttacttcat cacaacgttg
    30001 tttcagctcg tccaaattct ttgctgatac ccgtaccaca tagcttagtt tgtacataga
    30061 ttctttggtt tggtctaaat tactttctaa ttcatcgaca gattctaaag cgtccaccac
    30121 attagtactg gtttcgttgt cgctttccca tgcgtggcta tctaaatctt tcagttcttt
    30181 ctttttattg cgaacggtag ataaggcttt tttattggcg acaatttcaa cattcatact
    30241 ggtatcaacg gggaaagtaa actgctgttg ctggtaataa aaaatttcac tattagggaa
    30301 atccaattca ccaacaatac tattaattgt aaaatacgcc acataagtag tggtatcttc
    30361 ctgttcaatc cgtaaatagc gttgattttc ttcgattaaa cagcgagtgg gtttgagaat
    30421 atcgtatttc tttaccaaca cttttttatc ttcgtatcgt ttcggtaaat ggtagtcgta
    30481 gtcatcaaaa gggacacctg tttgcccgta aagatgttca ataatataac caaaatcatc
    30541 tttctccaat ttacgaatct gaaagcgtct ggataattta ctttctaaca actgttccat
    30601 cttagaaaag cgttcgattt ccgccatagg catagaaaca aaatcgccca ttaatttgtg
    30661 attcacacca tgaaagaatt ccgcaaatgc tttttgaatt tctttcccaa cattttttac
    30721 ggatacttcc tgttcattga ggtagagttt aaaaccaata aaaaagcgat agtctacttg
    30781 attgtcacca atcatagaaa ttaacgcttc ggtctgttca tctatttttt cgtaagctat
    30841 ctcttttaat cgtccagtga tttctttttt cgattgttcc tgtgtggctc gaatacttga
    30901 ttcggttgct agttgcagag catgaatctt gccatcacga ttttgggcaa tcaattgaca
    30961 gaaattatca tgaacttgaa ttttttcatc ggaacttaaa aaactgtaat tatagggaat
    31021 taattcataa taagcgaata cttcgccatc atgattaaat acgagattat cttccatata
    31081 tttaatcgga tatgccatct agttgttcac tccttacaat cgtaatcgat tcattcattg
    31141 tttctttttt taatcgtatt tgtttgccag cgtaggttac tttagggcgt agagcatagg
    31201 taatgataga ctttagaaat ccatagggtt tctttccgtc aaaggtcttt tggcacatga
    31261 acccagtgag aacaatggga atccctaagt atttgagtag tgctccgtca atcagagaaa
    31321 gtggtggtaa actgccaaag agtatgacga caaacaaaga aacgataaac catgtcattt
    31381 gcgtaaaggt aattgggaaa gggagttgta aatcattaat cgcataaatc actttttcta
    31441 ctgaccaaat actggtgtaa cttcttattt ttttcatgtc ttcacgtcct ttccgtaaaa
    31501 atagaaaaag aacaatcaaa ataggtgatt gctctttcaa tgctttaact attcaatttt
    31561 attttttgtc cattttgcaa taataccttc atcagtttct tcatcatctg gattcattga
    31621 atatctgaaa cttaaattgg tatctgaccc taatcgttca tataagctgg gtctatacaa
    31681 tctcgtttta ttgtcattaa aaggacttcg ttttcccagc tcaccatttc cctttaaagg
    31741 atttcttcta actgaaaaac aatatttttt tctgggatct tctgaatcac tttgacttaa
    31801 tgagaaacac atggcttctt tttggtcttc atttttcttc tcactaactt ctgtcggaat
    31861 aaatcgagca agagtttggc taaattgaaa taaaacatct cccaagttac tactgtattc
    31921 gataccaaaa tattctctta ttgttttcgt gtctgtatat aattttaccg aattagcata
    31981 gacagctagc atatctgaaa aatcgttttc ctttaaaaat tctagtttta acagagcata
    32041 ttctggaact ttttcctctt tttcaaatag cttaacaagt acaatataat tttgctgttt
    32101 gtaacgaaag taaaaggaat ctatcaccca gcctttatct ttcatgtcta gttttaatag
    32161 tttaagattt tcaaatacat ttaccataat ttatacctct ccgtgctatt tattaattta
    32221 attataacac ggattgttaa acttactgac aatattcaaa taccccatga gaggtaacta
    32281 ggtaattacc ctctatttcc atatcacgtc ccaaagattg gtagtcaata taattttgta
    32341 aattggaagg gacttcacca agctgtcctg tttcttccac gtaataacgg gcgacatctt
    32401 ccatcgaatc acaatcacta taacaatgaa tatcctcttt gttttccagt aattcttcca
    32461 agctactaaa ccacatgcct tgaatttctt tgagttcatt ataaataggt gtaccttcga
    32521 tttcttgaat ggcttcacat agacggttga tttcactaat cggcgtgtat tcgtccacat
    32581 caaaaggaag ctcaaaatca tgtattgcat attcttcgta gtcctcattt aaaccgattc
    32641 tttctgccat ttcttcctca tcaattggtg gagtaaacca tgctccgact aattcacctt
    32701 cattataccg tcctaaattt gccacataga ctttcatttc caattcttat cacatccttt
    32761 ttataaaaat agtcataaaa aaagtagcaa tcagttaatg acgctactta tcaaggtatc
    32821 cgctaatata atctgtaact gtacttgaaa tacctattat tagttttgct tctctctttc
    32881 caataatatt tgtattaggg tgtgcaagtg aatgcctatt tcttgcttca ttaagagagc
    32941 taatcattcc atttgatgac cttatcgttg tcttcactat atcagcaagt tcagtaggtt
    33001 gaatttcttt ttcaattaat tgttgtatcc tactatataa tttagataga ttttcgtctc
    33061 taggaactgt aatttcatat ttttttagta tttcaattaa atatccatga aaagcagtgt
    33121 gaactctgtc aaaggcacgt tgatattcac cattactcat aaaagactca gcgtcttcaa
    33181 tagcttgtga aaccacatca ctaattttca actgactaaa gttaattact tcgtaattct
    33241 ctggaagtgg tactaaaata gtatccacta atacattatc accttgttta tcatatatgg
    33301 agcttgctgt ctgggtaatc aaatctttat gactttcaaa aaagtctaaa tcatcaaaag
    33361 gaactcttat tttaagatat gtgcaaagcg tattccagcc ctttttatat cctttataat
    33421 aagtaaaatc gtcagtctca tctaaagtta cctctgatat tttcatttta gaaacaatct
    33481 cgttacctcc ttcataataa tttgaagagc tctttctttc taatgtttta attaaaagag
    33541 atttaaattg atcatttgta aacatattca taaatccttt cataacaaaa tatatacttc
    33601 ctattttatc atgaaaaata tctgttaagc accaatgatt cggttaaata agtttaataa
    33661 cacatctttc acgccagctg cattgaacac aagtccgacg gcgattagag caataatcaa
    33721 gaagccaatg agttttgaaa attctcgttt aaaacctaaa aacagtccaa tgacaacaat
    33781 cgctaacaat accaatgact gtgcattatc caagaaccag ttgtacaaat tctgcccaaa
    33841 attcatgtga ttgtcctcct ttccttaaga tatttttctc gataatagat atagcttttt
    33901 acacgcctta ataattgaaa aggggttaaa tgctctccat agacggttac tcgattggta
    33961 taattattat aaaatctcac cttgtaaaaa tcataggtat agatggtatc accagcttgc
    34021 cgttttccct caataatgag agacgtatat ttccctgtat tttgtatata cgcttgaaac
    34081 tcaatgccac actgaaaccc taacttcttt aattcttttg ttaaaaggct catgtaatca
    34141 aatcctccat ggctagatgt tgttgttcaa ttaatttctc atgtttttct gtcagtttgg
    34201 cttcctgaat catggtggag ataatggttg taccgttgag aacatctaaa atgcttgcga
    34261 tttttaatgt aggggcgact tgatgatgta accagttcaa tgttcgttca aaagaataag
    34321 gctctggctg tgtggttagg cgtaatgctc cacgattttt accgataaac cattcccaac
    34381 gttcattagt tttccaatca ctccgacgtt tagtgtcgtc tttatctgca aagcggatat
    34441 agcgattgat aatttcaaag gctgtttttt ccgcctgttg atgtgccaat aaatctttca
    34501 tggcttgggt ggctcggtca ttttttaacc gaatttcaaa ccgattcttt acttctgcgt
    34561 cttctaaggg aacatcattt ttcacgtatt gctcataatc tttctcatat atacagaaat
    34621 agacttcact ttttaatgag ccaatataaa gtgtgtttcc catgtatggt ttttcatcac
    34681 ggtgaaccaa ttcaccactg cgataatttt taaagcttcg aaagacggaa atgcattctt
    34741 cctgtttaca tttctttgcc aaatctggaa tattcaaaat ccctgtttta tcgttgatag
    34801 ctaaatccaa gcgtttgaaa atacctttat gttccatgca atcttgaaag aaatcatacc
    34861 agctccgatg ttgagccaat agaaagtttt caaattgccg acagcctttt cctttcagtt
    34921 ctaataaaac acctttactt acatcatgag agactaggac aacaatatca ccgaaacgat
    34981 agtgttcggg ataagaataa tagccaaagt cttcgtgtag catataatcc atattcaaac
    35041 gtaaaacctc accgataatt tgagccacat cattggtagg gaatcgaatc cgtacatagt
    35101 cgaataacat ctccaatggg ttatcgggat tgaagcgttc caaagcgttc agtaaatctt
    35161 ctttcacctg ttgagtaggg actgccttgc ctgtttcaat atccgatagg taaggtctgg
    35221 taatatgaga agcaaccgcc agtttatttt gagaaacgcc ataagccagt cgcttttctt
    35281 tcaattcctt aatccaaatt ttttcgtttt ccattcgcaa acctccaatc taaaaaagat
    35341 gtaaactatg acccttgagt ttacaccttt catattttcc gcaatccttt gtaccatcaa
    35401 cattggttga tgagaattgc ctgttttcag ttgtatttgt gcccccctgt tagatagagg
    35461 gggctaaagg attggcgtgg ctaacgccac accaatcaaa gcaagtctga acctgtacct
    35521 ttcacttcgt gcggtacagg ctcagattgc tttttttgaa tgacttttcc tatttctcct
    35581 aagaaatcgt acctttttgg tacaaggggt gtataaaact cactaatcac actttttcct
    35641 gtatccacgt aacctcttcc cttgatgggt tttaagaaaa attgtttttg tgtatcgctt
    35701 ccgaacatca taccaaagcc catttcagac atacgaccca aagccacacg gaagttaaag
    35761 ttgtcccgaa tcccatcact aaaatatttt gcgtctggac gttgacaggc aagaattaaa
    35821 aagaagcctg cttgtcgtcc taacattaca atttgtttga gtttatgaat aacggtggta
    35881 ctttcacgac ctagcatatc catcattgcc acatattcat caaaaataag aaaattggca
    35941 gacaaaccaa gataagcata attttctcct gttttatagt tagggtgttg tttcatggtt
    36001 tcactgcgat caatcatatc ttcgtaaaat tgattgatac atgccaacat atcttcttta
    36061 cggtaataga cattattcat gaccgcccct aagtctgcca aatcagcatt tttagggtca
    36121 agaatataaa gctttgcatt tgtatgcagg agagattcaa tcaatgtgag aataaaatag
    36181 gattttccac taccagtgcc cccagcaatt aacatgtgag ggagagaatc gaactcccaa
    36241 tagaccgatt ccatcaattt gacacgtcca tcttgagcta ccacgtcttc aatgccaatt
    36301 cgattgccaa tagtatcata aagtaggaca tactctacat aagaatcttt caacgttttt
    36361 tccactaatt cacaatacaa tcctgtttct agtttgtttt ctaggtgcaa aagctgatct
    36421 tgatatttcc ctaaggtgat ttctacatga atatggatta gtccatcttt cattcgataa
    36481 tatattttag gaaaatggcg gattcgttgt ttggttttct tgctgggtaa atctttaaag
    36541 aatccctctg attgagtttc ttcggcttca taccaatgat tttctaaaat catacgagct
    36601 aacttttgac ggtgctctaa ctgttttata gaatctatac gatagtgata ataaagccag
    36661 ctgataagtc cgcatacaaa aagagcagtg agaatactgc tccaaaagta tggcgtatga
    36721 agttgataga aaaatgtatc taactgaact gcttgccagt tcaccgattg taacgctctc
    36781 caatgaaaag ggagtaggac gactagaaaa actaccagca atgaaattag tacaaaatga
    36841 tagaccaagt gcttatcgtt ggctttgatt cgctctcctc tgtcatgaag caatggcata
    36901 aaaatcctcc tttccaccta tcaaaataat tgtgtattgt agatacgagc ctgtttatca
    36961 aaagttggtt gagaaatggc ttcatatttc tgataagcga ttcgcttttc aaagcgtaaa
    37021 tttttttctt tcggataata atgacgtcga tagagagtgt gtcctaacag ttttttcgct
    37081 tcttttgtca acggatagag atagcgaaac atgcgtccat tgattttctc aatccctttg
    37141 tattcgcaaa atgcctggga aagccaatgc tttttctcta cctgttcaaa gcgagcattt
    37201 tcctctaata aaaggcgagc acttcgaggg tgtatctttt cccatgactg cttatcacga
    37261 tagacacttg tcttgaaata cccgcagtaa aagaagttgg acgcttgata gacatagcca
    37321 cattttccct caattccatc tgccagtgta tataaaaaat gacagtccgt atgttctttc
    37381 aaccacttaa ttagagctga aagagcttga ctgccaaaat aattggtttg attcatttct
    37441 ggaagaaaac acatctttcc aatttctaaa taatctgtgg tttgaaggga actgtcggga
    37501 aagagtttgc gtatggtttg taggggttgc gtaccccagc ctaattccac aacgcctaat
    37561 aatttttctt ctgaaaaaat ccctagaaag tatttacaca atctagggag tatctttgaa
    37621 taatggtatt gccgaataaa agaaatggct tcatccttcg gaatttcttt caaaatcaat
    37681 ggtgtgtttg tcataagcaa cacctatttt tcactttcgg acgcttcttt tttatatttc
    37741 tgatgaggtt ttgtctgtcc ttttaataca agatcgtctg ctttcacgta ccaatcaacg
    37801 tctgcccctc gaaaagtagc cgtcgctacg gtatctgcca caggattgat taattccact
    37861 tctgcgttgt agtcaaattc tttcaaagga acagaagcgg ggatactgac ttgtatcatg
    37921 cgtccttgtc cttttgattt taagtcatag gtacgttctt taatttcttc ggaaaccgta
    37981 ccatcttcat tctggatacg aacctcacga cgcaaggcgg aaaatttcaa tgtgccaaat
    38041 gtctggtctt tttcaatcac aataccttct gctaatctca tattttttca cctttccttt
    38101 cttatgcttt caccatatcg tctgcgtgca taatgtaatt ggtaaatcct cgattgccaa
    38161 ttttataacc ttctgcggta atatgtgggt tgatgagttt gactttttcg tcggattcaa
    38221 aatgtttctc gccagcttcg gagggaagaa tgacaacaat atcatctgct cgttgaatcg
    38281 ttgagtataa attgtaacta cgagagagaa tggttggtct gccatttatt cgacgttgtt
    38341 caatgttccc ttcaccagca tattctaatt gtccaaatgt tttttccata ttgggaatga
    38401 catatttaag ttccatattt ttacctatcc tttctgtttt tatgtttgaa tgaatcttat
    38461 ttgtgtgatg gttttaaagg ggggagagcg accttttgat tcttgatttt ttattttcat
    38521 aagttcactt cctttcaaaa ttgggtaaaa aaatagacgc tccgtactga gcgtccgatt
    38581 cctactatat ttaaaacaac gactacctag gtattttaaa tattataatc aaattctatt
    38641 ttatctaata attgagtggg ataaagcaag tctcctttac tcgtaaaata acccacctct
    38701 gagaaaagaa ataaattatt ttttgctctt gaacaaatta cgtatagtag ttttctcgct
    38761 gaactaattc ctttatcaga aacattaaaa atttcattcc aatgaggaat tttcccgttt
    38821 agaagattaa tagcgataac agtatcatac tcatttcctt taactttatg tgctgtagta
    38881 agtgtgatac catctcgaga tttaaacatc ttcttaaaac tagatacact actagataat
    38941 gaattatttt ttatccgatc ttctacttcg ttaataaaaa atagatagtg cttaaaaata
    39001 gacgggtaat ttttctcgtc aatattaaga ttaacaaata tatgtttggt aacaaatttg
    39061 tataaatcaa caccgttgtc gtctccacta tacattcttt tcacactgtt tattagctgt
    39121 aataaatcta agtcaataaa ataatcttta acttctattc caaattcatt ttttaatata
    39181 attaaaatat cgtttgcaat tagctttctt cttcgaacct tttcacctga ttcagtaaag
    39241 tacaaaattg ctaacttgaa aaataaacta tgattattta ccttaattgg tgatatattt
    39301 atagcatcaa aattgcaata cggtaattct ttctttaaat actcacccag tgaaaacaga
    39361 ggatagtgtt gaggagcaag tacacaaata tctttttcat gaatgccgct ttcaatcttt
    39421 tcctttatta tattttttat tatgttgggt aaatcctcct tagtaacttt gcaatcataa
    39481 cttataaaac cacggtcgtt tttattattc cctctgcttt caatctcgta aggacaactc
    39541 tgaaaattag agtagaagtc tactaatctc tgattagacc tgtaacagcc atgtagcgtc
    39601 tttgattgaa aagtgtgact agttagcatt tctagttctt caactgattt agctatacca
    39661 tctaatgatc cataaattgc ttggtcaata tctccaacaa acattgactg aatttgaggg
    39721 ttgttttgaa gtaatttagc tattacattg tattgtaatt ctctagtatc ttgaaattca
    39781 tccacataaa tcgcccttat attactagca atattacgtg caacgttact gtttacagtt
    39841 aaaattttat acgctactag taaaatcata tcaaaatcaa tttctttatt tttttccaac
    39901 atagtgtgat acatttcaga aacttgagta tactgaactt cattaatttt aaaatcatga
    39961 gaaagtgaca aatcaatttt gttcagttca tacgttttga gatttaaatt cagcatatta
    40021 ttaatttggc tgacgtaagc tctttgagta taatcatcaa taattgtaaa tccttttgat
    40081 actcttgaga gattcatttt aaaaggataa ataataaatt caagacaaaa ctgatgtatt
    40141 gtccccaccc aaatattaat atcatcatct atactaagta aacttaggcg ttccttaatt
    40201 tcttcagcag ctctattagt gtatgtgata gcaattattc tttttagtga tccatcgtgt
    40261 ttaattgctt ggtaagcaat tttccttgta agtacccttg ttttcccact cccaggacat
    40321 gcatttacat aaaggttatc ttcactttcc acaacatctt tttgttctaa atttaaatca
    40381 tttacgattt tttcgaagat atttgtcatt ttgaaacctc agcataaagt atttttgtgt
    40441 aattttctat ctgattgatt agatacacaa catcctcatc tttaccaaag tactctaata
    40501 aagaaattat tgctatattc aaatcagctg tatcacttgt ttttaaacta gctgataaag
    40561 ctgacaattc tgtagtttcc tcgtacaagt cgattgtatg gagtagaatt tttttataaa
    40621 ttgagagatc tactatttcc tgacatgcaa aagcaatagc attaagaata tacttaggaa
    40681 ttttgattaa aaagtcaatt ttttcaccta actcaattgc aaaccacccc ttaccaacat
    40741 gattggctag ttttaacatc gctaaatttc tttctaaata ttgttcactt tgtatttctt
    40801 ctttataagc agttattgtt tttttatctt tgaatgcgtc atctataatt ggactgacat
    40861 aagttagaat attttcttta ttcacatcta aaaactcaat ttcaaatgtt gtattagcat
    40921 aaaaacaatt aacccaattg ttttcattaa aaagggagga taatttttct tttcttgctt
    40981 gacctttctc ttgtgcagtg cttttataca atcgatgttc tgatgggatt gcctgttcat
    41041 ctaaatcagt tacaatagca cactttctct gtattctatc atcgtcaaac aagctagcga
    41101 tatattcaaa cgaagtacta ccaacattaa tcaaacctat tccaagttcg tctaagttta
    41161 taccaaataa tttttttact atttgaggaa taagtatttc ttcagcatct ccttcaacta
    41221 agataacacc tttagaaaaa agcaatccat ttcttttaac atctaaatat ctctctactg
    41281 cgtttattag atttagattg ttctttttta acttttcttt agcaaatgag ttcagattat
    41341 ttgctggatt cataacgata gatttgccat taaacgattt taatacattc atccgagata
    41401 tttcagacac ttcagctaaa tgtacagaat gtgtggtcat gagaatctga gtatagtttt
    41461 tttgaagatt taatccttcg aacagagttt tttgaatatg atgatgaata tgagcttctg
    41521 gttcttcaat caacataata ttaagtagtt ctcttgaacg acacgcttca aattcgacta
    41581 ttttcaaagc taaataaatc atatttagat gtcctaaccc tagtaagtct aagtcgatat
    41641 tttgttctgg cttcattgaa agaaattttg ctaaagaacc catatcatct gacatagttg
    41701 aggaaagaag aagattagga gaatacactg aaccaattat gctatttagt tgccgattaa
    41761 tattttcgcc aattttccta atttcaggaa cactggtaat agtctcattt aattgctgta
    41821 taattgtttt taatgactca acattatctg atgatatttt tgattcaatt gtttccatga
    41881 ttcttttgac tgggtttcga ttgtttttca tttctctaag aacatctcga agagcgtcaa
    41941 cataaacaac cgaaatatgc ttgaaaatat ctgacatgtc aattcttgag cccaacagag
    42001 cttcatcgtc ttcttcagga ttactagcta tattgtcatt gagtcttcct accaatttat
    42061 cataattttc attgatacag aaatttagat ttgatttaga tgtgtagtaa aattcatagt
    42121 cggatagacg aatagaattt ctaaattcgt taaattgatg agagtctaat tcagtggaaa
    42181 tatcaaataa ttgctttcta atagctttgt taggtctaat aaatagagaa atactaccta
    42241 cacctttatc tgcatttgaa atcaattctt ctaagattgt ttgactttca gaatttaaag
    42301 aaatggaagc acaaatctca ttgtcaaatt cttcttcgct gattccttca aatgtcgcag
    42361 agataataat ccaatgccct tgccacccat tttgaattcc atgaaaaaaa tctgattctt
    42421 ttagtgtttt actactatag taataactat catctaataa gattctcaat gcagtaagag
    42481 cattggtttt ccctgaatca ttctcaccta taatagtatt tgtctcatta tcgaatcgaa
    42541 agatttcgga ttcataattt ctaaaatttc gaatttgaag agtagatata aacataagca
    42601 gatctcctaa ctatttaatt aatatgatta tatagtaagt ttatcataaa ttatgttagt
    42661 aaaagttctc attataaggg agcgtcaata attttgtgta aatgattctt ccctactgca
    42721 gattgtttct aattctcaat cagttggctt agcatttgtt cgagctcgcc ttcggcctgt
    42781 tggaaacctt tatgactgcg acctaggaat ttttggttgt aagtatcaaa agacgagact
    42841 aagaaacgtt ccatcgactc ttcattttgg aactgttcct tgcggtggct gtatttcttg
    42901 atttgtttat taaaggattc aatcaagttc gtagaataaa tactcttgcg tatggccaag
    42961 gggaaatcat agaaagtgag caagtgatcg ttcgaaagaa tcgattcaac cacttttgga
    43021 tagctggttt tccatttttc cgcaaaagta ccacgtgctt ccaacgccac ctctttagat
    43081 gaggcttgat acaccatttt aaaatcatca cagacttcct tacgatcatc gacacgcact
    43141 ttatgactga tattacggga aacgtgtaca caacaatgtt gaaaacgagc tttgggatag
    43201 aaccgactaa tcgctcctac catccccttt aagccatccg tgatgaacag gaggacattt
    43261 ttcaaaccgc gctcttgaag gtccaataaa atttcctccc aaatcgtgat ggattcagtc
    43321 ggtgcaatcg catagctcaa tacttccttt gatccgtccg ggcgaatgcc aactgcgata
    43381 tgaatagctt ccttggcgac ggttttccgc tttaacggaa tatacgttgc gtccatataa
    43441 atagcagcat aacggtcatg aagctcccgc ccattaaacg ccgttacctc ttctgtaaat
    43501 gattttgtta tattggacat ggtttggggc gtgtagtaat gcccatacat tttctcaatc
    43561 aagtctgcga tttccgacat ggtaatacct tttcggaaga ggtgaatgac ggtctcctct
    43621 aacgtgtcat tcgtccgtct ataagcagga acagtctgtt gcttgaactc accattgcgg
    43681 tcgcgcggaa tctggagatg aagttcccca tactcggtct tgaccgtacg gtcataggag
    43741 ccgttacgag aattacccgt gttaaaacca atgcgatcat acttttcgta atctaggaaa
    43801 gccgttaact ccgttttgag aagcgtattg actgctttct ccaaatggac acggaataat
    43861 tcattcaaat cgcctttgtt cgctagagtc tttagaattt ctgtagtaaa atcgttcata
    43921 gggaagtcct ctcttctgtg aatgtgttgt ggtaactcta ttctacagaa gggacttcct
    43981 tttttctatt tacacaaaat attttacact ctccattata acaattgggt taaaaagaaa
    44041 atagatagta ttctacctca tatttatttg aaagtaatat ataaaagacg ttcaaattcg
    44101 aacgcctctc gttaaccaaa tattggattc agaatctagt tttcatatat aaacagagta
    44161 tgattaggtg caaaaattgc acttttattg cacttttatt tttatttata gtgttttact
    44221 gttctgtata ttgttttaac tcccttgata gtatcgcatt ctgttttata cactttacgt
    44281 tgaaaacaga gtgggaataa agagccaaca taatatggag ggggaatccc tataaatcaa
    44341 cattcttcaa tagctaaaat gcttgatttt cattaaaaat aagatggttc cctaccaaaa
    44401 gagactataa tataagttaa tttgtttcat tcttatcata aaaagaagtt ctcgaaaaac
    44461 gaactgtttt ccgagaactt ttttctatcc atctaagtgc tcaaatcata ttcacgacgg
    44521 aattctaagg ttaccctttc ttaaacaaac tttatgttat ctgcacaact ttttgcaatt
    44581 ttttcattgc ttcttacgaa atcgttgaac tctctttagc ataatacaaa gtgtagatga
    44641 agtctggtgc tttactcgtt ttggtaaaaa tgactggaaa tcgtttctgg ttatttttgg
    44701 caaaggcgag gtgcaggttc gattcctgcc ttctacgcta acgatgtggc tgaagggaac
    44761 ctccgatctt gaatcgaaac gatccgcagt ctattgtttc accaatgaaa caaaatcaac
    44821 gttcgtccaa gcatttcctg aagccttgct ataaacccca atttcacaaa cacctgttgt
    44881 aattggaatg tctttgattg taatttttgt ccattcactt gttttaacaa ccgcagtatt
    44941 tagttccgct ccaccataat ttttgatata taactgatta gtatcaaact ctcccccaga
    45001 cattacatag gcactcaatg tgtaagttcc tttgcttatg ccactaacat tttgataagc
    45061 tgatgcctcg tatgcttgat cctgccaaaa agttagtttg tagtccccaa aaaaagtgcc
    45121 cgcttccgtt ttgatcgtac ccttagcggt tccttgttga aaccaggttt gccaattgga
    45181 aggactggtc gttggtccat cgctttcgaa acttgcattt ttaactagat ttatttgcgc
    45241 ccctgcttgt ttaaacgcac gcaagcttgg caaggcgttt ccagcaaaat caaacatcgc
    45301 ttggttgtcc cattcgtttc ccatagtgga ttgaacactg agataattca tacctgcttg
    45361 tgttgcccaa ctcacatttc cgttgtacca aagaggttcc caatagaaga agcccgcccc
    45421 gcgattattc ggtactgcct gaatccgatc ggcaagatcc agcaaatact ctgcttgtcc
    45481 tgtcggtgtt ggttcatatc cccccgcagc tgcttcggct tcaccaaaag catttggcat
    45541 agtatcggca ttcgctgttg tatgcccata agctgtttca actacgatca cttctttacc
    45601 ataccgttgg ctgatcgcat tcatattggt ttgaagttca ttcattgaac cgtgccaata
    45661 cggataatac gacactccaa ttaaatcaaa tgacacacct cgactcgtga tttcatcaaa
    45721 ccaccaagta aatgctcctg tatcgccacc atcagctaag tggagcatta ctggagttgt
    45781 tttaggttga gtgtctcgaa cagcttggat accagacttc aaaaaagcag ccaatcgatc
    45841 aaattctccc ccgccttctc cccaagactt gccataaggc cagagcattc cgctattcag
    45901 ttcattgccg atctgaacca tatcaggata aacgtccagc tgcttcattt ccgacagaac
    45961 gttctttgta tagtcgtaca atgccgtatt catttcttca aaggtcagac cttgccatgc
    46021 tttaggaaga ttctgtttcc caggatcaac ccaaaaatca ctgtagtgaa aatccagcag
    46081 caccttaagt cccagatttt ttgctcgttt tgcaaggtca atcgtcgttt ccagatcatt
    46141 tgtccctgct ccataacttt ctccattact attataagga tcctgccaca aacgcagtcg
    46201 aacatagttg acaccattat tttttaaaat ctctaaggca tcttttgaaa tatcgtcttc
    46261 ataatatttt gcaccagatt tttccatatc tgacaggatc gaaatatctg ctccgcgaat
    46321 aaattcgctg gcagatgctt caacagtttt tgaaacagcc cccaaagccc ctaacacagc
    46381 tgcaaataaa acagctttac gcaaccaaat tcccattttc attatggctc ctcctttatt
    46441 gaaattggtt cagaataaac tgaacagctt tttcaggatc tctcgttaat ccgatatatt
    46501 ctgctccttt tgtcgcaacc agcttgattc ctaagcgatc ggtgtcttct ttgatatcat
    46561 catagtaaga attgacttgc ggtgctaaga ccaccatatc aaaatcctgc agcatttcgt
    46621 aatgagaacc ataagcaccc gccattgcac tgatcggtgc tttcgattca gatgcacctt
    46681 tttttaaagc attagctaac atcgcacttg tccctgcgcc agcacaaagt accaacacat
    46741 tgatttcttt atttgcatcg ctgataactt gactcgctgc cacaggtact tcctcacgta
    46801 catcggcaga agcaccttcc tcggttgcga tcacctctgc tttttgtgct tctttctcta
    46861 ctaagacagc atcgtatgct ttgcaaaatg gtagatagat caggaagtca acgacgatta
    46921 gaaccgctgc tagaacaaaa gctaagatac ccatgcccgt tcctagaacc aaaccgatcg
    46981 gtgctggagt tgcccaaggc aaaacgtaca taaagctgtt catgcccaat acatcgacaa
    47041 aaaacttgaa caggcaaacg tttacaacag gtgttaacaa aaatggaata aaaaaataag
    47101 ggttcaaaat catgggtgcc gcaaatagca atggttcgtt gacagcgaaa cttactggaa
    47161 taaaggaagc tttcccaact gccttcaact gcttagactt agcaaagagt aggaagataa
    47221 aaggaacaac caatgttgcc ccagttcctc ccatagttcc tacaaaattc cctaaaccca
    47281 ccgtcagtac tttagaagct tgttcaccgg aattgaacaa ttgcagattg gtttccacat
    47341 tggcatagat aattgccgca attgccggct ccacgatcga cggtccatga acaccgacaa
    47401 accaaaacag cgccatcgct ccccagataa tagcgattcc taagtacccg tcagctgctg
    47461 taaatagcgg ctgcagcacc gtaatgattg cttctgcaaa tgagatttgg aagatttgtc
    47521 gaatgcctaa atcaattaaa acggccgcaa atactgaaaa agagaaagga aaaatatctc
    47581 ggaaggtttg cgaaatcgtt ccaggaactt ctttaggcat tttaatggta atatctttca
    47641 gcacacaaaa cttatataaa ttaactgtta aaaatgcagc gacaaaggaa gccagtagtc
    47701 cctttgtccc catatattcc gttgaaaatc caccttcaat cggagagacg cttaacaaca
    47761 ggaaacttac cacagaggcc agcatcacag aggtggtgtt gattactttg tttttcggta
    47821 agcgacgatt catcgaatca gccaagcagc gagcagtggt tgctgaaacg agtaacccta
    47881 caacacccat cgaataatta tagactttcc atagccatgt ggagacagat tcaggccatt
    47941 ggtaaccaaa aatctcagga accgcagcaa tcaaaatgaa aatgcttgaa aataaaatag
    48001 ctggcatcgc tgttaaaaat ccgtcacgaa cggctcctaa ataactatta cgtgagattt
    48061 tttcaaaaaa tggtttccct ttttcgattt gtttaatgat tccatccata gcgttcttct
    48121 cctatccttg attctgtctg tacagtacaa taaagtcttg aatcagatct ctcagcaata
    48181 aagtggtcat taaatgatcc tgcccatgga taaaaatgaa tcccatctcc atctctttcc
    48241 cagatgcttc ttccgctaaa atttttgttt gcgaattatg tgcaagggtt aaattttcgt
    48301 cggcttcttt caacgccgaa tcaacatgtt caaagtttcc ttgtcttact tctttcaata
    48361 atttcaataa tgtactgcga gcgtcgcctg aatatgcaac gatctcaaaa ccaagcattt
    48421 gtaattcctc tttattcatc ttttcttcct ccattatcac ttattaaata atcgttttgg
    48481 tttcactgac caacttgtac cagtaagctg attcttttgg gtaacgcttc tgggtttcaa
    48541 aatcaacata aaacaagcca taccttttgt tatacccatt tgtccatgag aaaagatcca
    48601 taagcgacca caaaaaatac cccttcacat ttaccccggc tgttatcgcc ttgcttaagg
    48661 attccaaata gactctcaaa tagtcgattc tcggctgatc cataataatg ccatcttcaa
    48721 actgatcttt ataccccatc ccgttctcag tgatatagat tttgttgtaa tgtgggtaat
    48781 cgcttttaat tcgcagcaac aagtcatata gaccttctgg ataaatcagc cagtcccaat
    48841 cagttcgagg aattccttct ttataaatac gttctccgat tcccttcact ttataaacag
    48901 aggtcccttt ttcacccgta ccgttatgat ggattgcatt ttctccatcg taagctttga
    48961 caaaatggca ttggtagtga ttgatcccta agtagtcatt acgcgtggat gcttttttca
    49021 attcaacgaa atcttcttca ggaaaatgat agctggcctg atttgcttca catatctcat
    49081 caagagcagt caaggtttca gttgaataat agcctaaata ggtagcatct aataagaaac
    49141 ggatcgacaa tgcatcgtct aaaaaggcag catgcttatc ttccggcgcg tctgttgccg
    49201 catatttcgt ctctaatgag tgaactacac caatttcacc cggaagttcg ttttctttga
    49261 aatagttcac tacacgggca tgagcaacca tcatattatg aagacaggcg acgatctttg
    49321 taaaatcata tttgattcct ggtgggaaaa cacctaacaa atattgattg gttgcaactg
    49381 gatagatttc attaaaggta ctccacacct tgacttcttt gaattcatga aaacaaaaaa
    49441 tggcatagga aacaaatgct tctattgttt ctcgattcaa aaaatctcca tggtcaaaca
    49501 aacgtttagg ggtatcaaaa tgatgcaacg tcacaaacgg cgttacgttt cttttcttac
    49561 attcttcgaa cacacgatga taaaaagcga ttccttctgg atttggtttc ccagctccat
    49621 caggaaaaat gcggctccaa gcaatggata atcttagacc atttacacca aaacgttcac
    49681 acaattcaat gtcctttgca tattgatgat aaaaatcact tgccggatcg ggactgaatc
    49741 gcccttgttc ctctaaaaag tcatcccaag caacagcgcc tttgccacct tccttagtgg
    49801 ctccttcaac ctgataggct gctgtggctc cgccaaaaat aaaatcttct ggtaatttaa
    49861 gcatgtacat ctctcctcaa atctataatc agttttcgct ctttctaact gattgcattg
    49921 taaacgcatc caaaacattt gtcaacaaaa acaaacacaa ataatcataa aaaccagaaa
    49981 acagcaataa aatctattgt aacaaacata aaaaccaccc taaatgttta cttttgttcg
    50041 aattagattg actgttgtcc gtttatatga tagattatcc ttaaattaga ggtgaagaca
    50101 atgttgaaaa aagaaagatt gcttactatc gtagaaatgg tcaataaaaa aggaatcctt
    50161 accgttaatg aaatcatcaa tcaattagat gtttctgata tgaccgttcg cagagattta
    50221 gatgaattag aaaaatcggg caagctgctg cgtgtccatg gaggggctca aagtctttcc
    50281 tacactttag atcaagaact ttctcacact gaaaaagcca ctctgcagat tcaagaaaaa
    50341 aaagaaattg ctgccacagc agcctccttg attcacgagg gagaaacgat tttcttagga
    50401 ccaggtacaa caatcgaact attggcgacg catttactga acaagaaaat tcggatcatt
    50461 acaaataatt atccagtttt tgatattctt gctcaagcag atggaccaga aattctactg
    50521 attggcggtg atttccggaa aaataccggc gcatttgtcg gaccgatcac caacgacaat
    50581 ttaagaagac taaagtttac aaaagcattt atcagttcaa atggcgttca caacgaagcg
    50641 atctctactt acagtaccga agaaggagaa gcacagcaga tcgctctaaa taattcccgt
    50701 aagaaatatc tactcgttga taataaaaaa ttcaatcgag atgattttta tgtgttttat
    50761 aatttgcatg atttcgatct gctcattacg gatcgccaga tctcgcggga ggttcaagag
    50821 cactatgcac aatatgtcga tctgcaaatt gctgattcaa ccgaaaatac cactatagga
    50881 gcgtaactat gaaagggatt atatttgatt tcaacggaac aatgtttttg gactctcatc
    50941 tgcatgaagc tgcttggctg catatgatac atgatcatac aaaagatggt ttatcagacg
    51001 aggatatttt aaaaaatatt catggtcgga cgaatacgga gattttgaaa catttcattt
    51061 cagagcattt gaccgcctct gagatcgccg tattgtcaga agaaaaagaa agctattatc
    51121 gtatgctttg cttgcaaaat gaaaacgagc ttcagttgac aaaagggctt gaagcgactc
    51181 ttgaccagct gactgaccaa cagattccat taacgattgc cacggctact gtcaaagaga
    51241 atgttgcttt ttattttgat attttcgatt tagcccgctg gtttgactgg gacaagattg
    51301 tctatgacga cggctctttc cctgggaaac cgcaaccgga tatttttctt aaagcggcag
    51361 acaaactgag cttagctccg gctgattgtc ttgttatcga agatgcttac tcaggattac
    51421 ttgctgccaa acgtgcgggg atcggaacaa ttattgcaat tgatccattc ggtaaaaaca
    51481 aaacagtatt tgaacaggca cagctaaata aagatggaac catcaaagat tttactggtt
    51541 tttggacaga gcacctgtct gccacatccg ttaattctta aacgattcaa ctaactacca
    51601 aagaaaagca actgaaatgc acacctcctt ttataaaaaa ctgccagtga aacgacatga
    51661 aagatacact gctatatcct tagtgaaata tagcagtgta tctttgactt tttcattctt
    51721 ttaaccatat gacgcgttca tctctatcta taattaacat taaaaatagt gttttcacat
    51781 tatttctcaa accaatctta aaaatcatca ggagtacttt tttgatatac ttttattaat
    51841 gctgggcgta atggttaaaa gatttccact agttcctctc caccaagctg agttgaaaaa
    51901 tgcttatctg taaacaacat agacaaagag gaaataaacg tttccgtatc tttctctgtc
    51961 ccaaggatat taattaattt tcctactagg taagtatcat taaacttaag atctgccttc
    52021 acatcaaaag ttttgataaa agcttttatc atgcgtacaa ttttgtcttt atcgccataa
    52081 ttgatttcac cactcatatc aatatagtca ctaatccctg aagaaataat ggcaactgct
    52141 tcttctttcg ccttatcaga taattcattt aatgactggg attgattgat gacttcttga
    52201 gttttatctg ttacgtaatc tctcattatc ctatcggcat actccgcaaa gtaatcttga
    52261 aaaaattctt ctacagggta accaacttcg ttttcccaac caggagaaat ttcattatca
    52321 gtacttatta aatctattaa aaaccaaccc gttctctgag tgttgattct ataatatttt
    52381 taatttccta tataaatcaa aaatattata gaatcaataa gctaattaag aaacaaactt
    52441 tagaaactct agaagaactt ctgaatttat atagaagtgt gtagctactt actaaatata
    52501 caaaagttaa aataaaatac tctggatact ctttatactg cattaaagtt agtaatatta
    52561 acagcaaaga tatcgaaaaa acaataacat aatatgtgtc aaaatttttc ataataagaa
    52621 ctcccttact gttgcatact aacataatcc aaaaactctt ctacttcagc taaagacatt
    52681 ccactttctt tagtccaaaa aacagttttt tctatcgatc cttctctgta aactgtaaat
    52741 gtcggagtcg agttaattga gtagcgactc ttgaattctt tccactcatt ttcatcttct
    52801 ctaacttttg ctacattcac gtaataaata ttttcttttc ccttttctac cagcatatcg
    52861 aatagcttcc tgcttttgat gatcgatttc ttgtgctaca tctagtaaat tttcatcaat
    52921 ttctatatca taaagtattt cactagtgtc tacatccaca atatatactt tttatcagta
    52981 gcaatttttg agtaatccgt taaaatgatc tcagtctcgt cactttcatc agtataaata
    53041 tacgttaaca atgaactcac cttttcaatc ggtgttagct ctaagcctac tgtatttttt
    53101 actattgagt cattatttac caccataact tcgtactgta tataccatcc tatttggttt
    53161 ccatcaatat cagtaattgg ctcactagct aatggagtct ccggaacaat attttcctga
    53221 tatttcttaa actcttctgt agcacgaaaa aggttatcat gttcctcact tgaaactggt
    53281 tgagtcatag aaaatgttac ttggctaaac agagggaaaa taaacactac aatcaacaaa
    53341 gtataaaaaa aagcccttct ttttcatttt ttatcttcca atcacaaatt gaaaaactta
    53401 ttcctgcaac tttgagtcta gtatcacaat cgactgacta caactattca tttacattct
    53461 actttaagtt cctccttaac cattaatttt attaaagata cttgtaatag tgattctcac
    53521 aaaatttcat gaaaaatact ttattactgt atctaaaatt ccaatacaca acttatcgac
    53581 tattatctga aaaccaaaac ttattattcc ttgatgttat tctagcaata gtaactgctt
    53641 ttttattttt ttgcatattt tttcggcgtt actttttaaa gttaactttt tcgacaaata
    53701 gaaagagata cgaatcactg atttttttga agaaaagaaa tggagtgagc tgaacgttag
    53761 agatattggg agctttcgta actgcttatt aaacgataat ggttatttgc aaccgtattg
    53821 ctcgcttcta catgaaatat ttcatgaaat atttcgcaaa acctttgatt atgtagttac
    53881 gtttccattc ttgactaaaa atgtttcaaa aacgaacaaa ggtgttaaaa ggtaaagtga
    53941 tcaatactac tggtcaaaat aagaggttga aagggaattg aaaaataact acagataata
    54001 cttttataaa cgtacgtgct ttgtggttct gtagttaact atataactgg aattagggaa
    54061 tggtatcaat ttgaaaagaa agattctacc aatttatcat atgttaaaaa tggaaaatca
    54121 acagcagttc tttcggaaac cttacacaaa aaccgattat tgaaaatagc cgaaaaacgt
    54181 ttttcggcta tttttttaat taagtttcct tcttcttacc ttttcccact attccgtttc
    54241 acatctttga tgatcttcag cgcacgtttt cgtgttttga tatttgggct tttcaagcgg
    54301 cggtatgcac ttgagatatc tgttttttcc atatggacga cccactttct tctttgattg
    54361 tagtcatgct aaaaaacaca aatcgaaaca attacgattt gtgttttaaa tggtatcaaa
    54421 agttaaaatg aaagtcaact tgttaaaaca gtcgatcaga ctagctgatt gactcctatc
    54481 tctggaaata aattttcatt attacgtttt agctacctaa aaaaggccct cttgtccgtt
    54541 aactttaata tctattgtta tagctagttt acctattgaa cgtactgtgt gttcgataga
    54601 acac
//
LOCUS       DACCKU010000007        53630 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.007, whole genome shotgun sequence.
ACCESSION   DACCKU010000007 DACCKU010000000
VERSION     DACCKU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 53630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53630
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.007"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            1..390
                     /locus_tag="IVN13_000391"
                     /pseudo
     CDS             1..390
                     /locus_tag="IVN13_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            390..1319
                     /locus_tag="IVN13_000392"
     CDS             390..1319
                     /locus_tag="IVN13_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7010911.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            1316..3052
                     /locus_tag="IVN13_000393"
     CDS             1316..3052
                     /locus_tag="IVN13_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7010912.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(3115..3951)
                     /gene="pheA"
                     /locus_tag="IVN13_000394"
     CDS             complement(3115..3951)
                     /gene="pheA"
                     /locus_tag="IVN13_000394"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP7010913.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPACLRALFRKQV
                     DLAVVPVENSLEGSVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIIGDRKMTSQQPAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(3948..4457)
                     /locus_tag="IVN13_000395"
     CDS             complement(3948..4457)
                     /locus_tag="IVN13_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP7010914.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQRLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(4450..5745)
                     /gene="aroA"
                     /locus_tag="IVN13_000396"
     CDS             complement(4450..5745)
                     /gene="aroA"
                     /locus_tag="IVN13_000396"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP7010915.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEMIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFMTTLFGDASLSKRPMGRVMEPLREMGADLQGKENEQYLPITVTGTNSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRISVEGKTITVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(5758..6849)
                     /locus_tag="IVN13_000397"
     CDS             complement(5758..6849)
                     /locus_tag="IVN13_000397"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP7010916.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAI
                     EQQIVDYVANDFETGAEQADVILLAVPVRTTLAYLAVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADDKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMAQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPIHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(6866..8032)
                     /gene="aroC"
                     /locus_tag="IVN13_000398"
     CDS             complement(6866..8032)
                     /gene="aroC"
                     /locus_tag="IVN13_000398"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP7010917.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKVAVKEYRDYLKHY"
     gene            complement(8034..9098)
                     /gene="aroB"
                     /locus_tag="IVN13_000399"
     CDS             complement(8034..9098)
                     /gene="aroB"
                     /locus_tag="IVN13_000399"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP7010918.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKNLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(9131..10153)
                     /gene="aroF"
                     /locus_tag="IVN13_000400"
     CDS             complement(9131..10153)
                     /gene="aroF"
                     /locus_tag="IVN13_000400"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP7010919.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(10812..11786)
                     /locus_tag="IVN13_000401"
     CDS             complement(10812..11786)
                     /locus_tag="IVN13_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP7010920.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(11767..13650)
                     /locus_tag="IVN13_000402"
     CDS             complement(11767..13650)
                     /locus_tag="IVN13_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP7010921.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(13668..15893)
                     /locus_tag="IVN13_000403"
     CDS             complement(13668..15893)
                     /locus_tag="IVN13_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP7010922.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            16007..17041
                     /locus_tag="IVN13_000404"
     CDS             16007..17041
                     /locus_tag="IVN13_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7010923.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(17108..18121)
                     /locus_tag="IVN13_000405"
     CDS             complement(17108..18121)
                     /locus_tag="IVN13_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7010924.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(18199..18387)
                     /locus_tag="IVN13_000406"
     CDS             complement(18199..18387)
                     /locus_tag="IVN13_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP7010925.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(18827..18955)
                     /locus_tag="IVN13_000407"
     CDS             complement(18827..18955)
                     /locus_tag="IVN13_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7010926.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(18958..20331)
                     /gene="rlmD"
                     /locus_tag="IVN13_000408"
     CDS             complement(18958..20331)
                     /gene="rlmD"
                     /locus_tag="IVN13_000408"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7010927.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRAILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(20442..21497)
                     /locus_tag="IVN13_000409"
     CDS             complement(20442..21497)
                     /locus_tag="IVN13_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7010928.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(21507..22937)
                     /gene="gatB"
                     /locus_tag="IVN13_000410"
     CDS             complement(21507..22937)
                     /gene="gatB"
                     /locus_tag="IVN13_000410"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP7010929.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(22934..24406)
                     /gene="gatA"
                     /locus_tag="IVN13_000411"
     CDS             complement(22934..24406)
                     /gene="gatA"
                     /locus_tag="IVN13_000411"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP7010930.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(24406..24711)
                     /gene="gatC"
                     /locus_tag="IVN13_000412"
     CDS             complement(24406..24711)
                     /gene="gatC"
                     /locus_tag="IVN13_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP7010931.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(24817..26853)
                     /gene="ligA"
                     /locus_tag="IVN13_000413"
     CDS             complement(24817..26853)
                     /gene="ligA"
                     /locus_tag="IVN13_000413"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP7010932.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(26870..29104)
                     /gene="pcrA"
                     /locus_tag="IVN13_000414"
     CDS             complement(26870..29104)
                     /gene="pcrA"
                     /locus_tag="IVN13_000414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP7010933.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            29259..30818
                     /locus_tag="IVN13_000415"
     CDS             29259..30818
                     /locus_tag="IVN13_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP7010934.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(30929..32860)
                     /locus_tag="IVN13_000416"
     CDS             complement(30929..32860)
                     /locus_tag="IVN13_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7010935.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(32878..33792)
                     /gene="pfkB"
                     /locus_tag="IVN13_000417"
     CDS             complement(32878..33792)
                     /gene="pfkB"
                     /locus_tag="IVN13_000417"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP7010936.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKKDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(33789..34541)
                     /locus_tag="IVN13_000418"
     CDS             complement(33789..34541)
                     /locus_tag="IVN13_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7010937.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(34701..34910)
                     /locus_tag="IVN13_000419"
     CDS             complement(34701..34910)
                     /locus_tag="IVN13_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010938.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(34921..36249)
                     /locus_tag="IVN13_000420"
     CDS             complement(34921..36249)
                     /locus_tag="IVN13_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010939.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(36614..37201)
                     /locus_tag="IVN13_000421"
     CDS             complement(36614..37201)
                     /locus_tag="IVN13_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP7010940.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(37284..38534)
                     /gene="clpX"
                     /locus_tag="IVN13_000422"
     CDS             complement(37284..38534)
                     /gene="clpX"
                     /locus_tag="IVN13_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP7010941.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(38781..40070)
                     /locus_tag="IVN13_000423"
     CDS             complement(38781..40070)
                     /locus_tag="IVN13_000423"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP7010942.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(40224..41156)
                     /locus_tag="IVN13_000424"
     CDS             complement(40224..41156)
                     /locus_tag="IVN13_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010943.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(41313..41972)
                     /locus_tag="IVN13_000425"
     CDS             complement(41313..41972)
                     /locus_tag="IVN13_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP7010944.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(41975..43000)
                     /gene="sppA"
                     /locus_tag="IVN13_000426"
     CDS             complement(41975..43000)
                     /gene="sppA"
                     /locus_tag="IVN13_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP7010945.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(43013..43303)
                     /locus_tag="IVN13_000427"
     CDS             complement(43013..43303)
                     /locus_tag="IVN13_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010946.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            43712..45517
                     /gene="glmS"
                     /locus_tag="IVN13_000428"
     CDS             43712..45517
                     /gene="glmS"
                     /locus_tag="IVN13_000428"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP7010947.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(45589..46944)
                     /locus_tag="IVN13_000429"
     CDS             complement(45589..46944)
                     /locus_tag="IVN13_000429"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7010948.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(46968..48137)
                     /locus_tag="IVN13_000430"
     CDS             complement(46968..48137)
                     /locus_tag="IVN13_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010949.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(48134..49021)
                     /locus_tag="IVN13_000431"
     CDS             complement(48134..49021)
                     /locus_tag="IVN13_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP7010950.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(49210..52902)
                     /gene="nifJ"
                     /locus_tag="IVN13_000432"
     CDS             complement(49210..52902)
                     /gene="nifJ"
                     /locus_tag="IVN13_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP7010951.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLNLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            53101..53622
                     /locus_tag="IVN13_000433"
     CDS             53101..53622
                     /locus_tag="IVN13_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7010952.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT        14651 a        11637 c         9707 g        17635 t
ORIGIN      
        1 gaatcgaatt atcgcggcta ttaaaacagc aacaacctga aacacagatc cttatcctct
       61 ctagctatga tgaatttgat atggttaaag attcttttaa agaaggcgtc gctgattatc
      121 ttttaaagcc tcgattgagt cctgattttt ttctaaattc cttaactgcc gtttcaccaa
      181 aaatcaaaaa aaagcccaac aaaagcccaa tcaccaatcg ttatgagcag atctgcgaga
      241 atctttcaca gcagattgct ggacaaactc gtccattgct tgaccctctt ctttttgaac
      301 acaccttgtt ttttctgtta tataccgaaa aacggccatt cagttcaaaa caaggctcta
      361 aacaaaaaca gtcgatcacc cattcattga tggatagcga cgattcgttt actatttatc
      421 ctgtttctac cttttcggaa gaatccggct atgtaatcaa tactgattcc aaagatacac
      481 ttaggaagtt tgttcaaaag tttcgtcaag aagtttttcc aggtgatttt tttgttgttt
      541 ctgattcttt tgcaatccaa gatatcaatg aaatattcca agagatcaga aattgcacca
      601 aaggacaacg tttctatcat aagggaaaga accttgctta tcagcatgaa cttcttccgt
      661 tattaaccaa ccacgagcag caagcaagta gttatttaac aaaaatcgtt cataaagact
      721 acgctttagc tgtcaaagaa cttgagaatt tcttcacgac gatattggaa cagcctattg
      781 atccagctta tttgaaacat gaggcaatca atcgtttcta tgcattactc aacgctttgg
      841 ctgacgagca tgttgggaat gaagaacttt ctttgctcaa gatcgccctt ccttctttat
      901 tagccgatgc tgagtatttc gaagattttt ctattcttgt caaagaaaca cttgagaaac
      961 tcacccactt ttctgcaaat gcaaaaaaag acaatttcga tatcttgact ttgatctacg
     1021 agttcgtcca ggaaaattat gagaaagaaa tcaatttaca acttttatcc gaaaaatatc
     1081 atttttcata tagctatctc tcagccatct ttactgaaaa atttggtatc aacttttcaa
     1141 aatatctaaa aaaagtccgt atcagtaaag ccaagctctt tttgacggaa acacggctta
     1201 ctttgacgga aatctgtgag aaagtcggct ataccgaact gggttatttc tccagagttt
     1261 ttaaagaaga gacaggaatg acaccttctc aataccgtaa aggaactttt gtaaaatgag
     1321 aaaaaaaatc aaagagcatg aactctttgc caaacttctg ttcattttgt tcatcggtct
     1381 ttttctgcaa gccatcgtca tttctttgtt tatttatcat cgatcaaggg atgcctatat
     1441 ccagctgttc aatcaatcga atgatgtggt actaaaaaag attcaatctg aatttgaaag
     1501 tttgaatgat acaatcgaga atacattagc cgcttttgat tctaatccag ctgtcaaaag
     1561 ctatttttcc aatgatccag cccaacatat ggaacagctt cagcaactaa gaacaatcca
     1621 aaaaatgaat gactctcttt caaaaatcca tccaatgatc gattacgatg tattgatctt
     1681 tggagaaaat ggaagaactt ttgtcggaaa cgacatgctc actgccgtat ctgccgattc
     1741 tttttttcaa tctgcgattg ctcagagagt gaatgaacgt gctgctgata cacaaatgct
     1801 ttttgcacac cacggattga ctcttcgcga taagaaagca cctagcgtct tctttgtcaa
     1861 aaaactgaaa aatacattga atcacgttta cggatatgct gttttatcga tctcatccaa
     1921 gcaactagcg aatctttttc aatctgtagt aaatcctgag atatcgaaga tctccttcat
     1981 gaacgaagaa cagacgatca tcgcatcaaa tgaagaaaat acaattggga gccgatcaat
     2041 gattttcaaa aaactaacag taggtgaaac aacgatttca aatggacagc agttgacccg
     2101 tcttcctctg taccgccagg attgtacatt actcagtcaa gtcgatcttc attctttatc
     2161 taaccaaatg ggcgtgattt ttccgattat tttgtataat atcgtgacac ttgcttttgc
     2221 tggctcattg gtatttatgt atttggatcg acacacaaaa tcgatctacc gcttgattga
     2281 ttcgctaaaa cggctagagg aagatggctt aaaagctact gttcctgtac aaggaagcca
     2341 tgaagtccaa ttgctaggga atacgatcaa tcaattgcta agtaatgctc agcatcatta
     2401 tgaacagacg atccaagacg aaaagaagaa acgttcgctt gaaatccaaa ctatgcaagc
     2461 acagatacag ccccatttta tttataatac gctgactagc ttgaaatttt tgatttggca
     2521 gaaaaaaaca gatgaagcag ttcaaggaat cgacagtttc attactttac taagaagtac
     2581 gattgggaaa aaagaagaag tgattccagt caaggaagaa atcaaaagcg tacaaagtta
     2641 catcgatctt ttgagtatgc gatatggcga aagaatctcg gctaaaataa tgattcctga
     2701 tgaactgcag tcattatcta tgccgaatat gatccttcaa ccaattatcg aaaacgccta
     2761 tcttcatgct tttcacaata aaacgcaagg attcatcacg gtgtacggca aaacaaaaaa
     2821 tcagcttctt ttattcgaag tgattgataa cggcgatggc ttcgatactg aaaataccaa
     2881 acaaaaaaat gatcattttt caggaatcgg catcagccat gtagatgagc ggatacgcct
     2941 aatgtatggc gatgcatatg gcttaacgat ccaatcagtg attgggacag gaacgactgt
     3001 aacgatttgt ctaccgcttc ttcctaaaaa tcaatcagaa tatcaaaaat aaagaatagc
     3061 caagcactga tttttttatt ttaagttaaa taaatcaatc agtattcttg actttcatct
     3121 acgcgtctct tctactgtca gcactggata acagcccaat atttgacttt ctcctccgag
     3181 catcttgatt tcttggagcg cattctctac taatgtcatt gggcggtcca acaacaaatc
     3241 gatcacgaaa aaatattctc ccaaactggt tttcaatggt cgggattcaa tcttacttaa
     3301 gttgatctcc cgccatccga aagctgccag cattttatga agcatccctg gccgattagc
     3361 aggtagtgtc aagatgacac tcatttttac tggagctggc tgctggctgg tcatctttcg
     3421 atcacctatg atccaaaaac gtgtctgatt caactcatta tcttggatat tctcagctaa
     3481 gatctccaat ccaacatgct gtgctgtttc taatgaagca atcgctgctg cgtcttcttt
     3541 cggatgttct gctacataca ttgctgcagc agttgttgat tctgttgcta ctaaaggaac
     3601 attaggataa tgcgtttcta aaaactgttg tgattgtgct agtgcttgtg gatgtgaaag
     3661 gatttttgta atctttgttg ctggattgcc taaaagctgc tgcttgatag gaagaacgat
     3721 ttcactttta acttcgactt ctggatgttt ggaaagaaga tcgattgtgt gatgaacaga
     3781 tccttccaat gaattctcta caggaacgac agccagatcg acttgtttgc gaaacaaagc
     3841 gcgcaaacaa gcagggatgg aagcatacgc agtcaattgt tctgtaggaa atagctgaca
     3901 agccgcctga taagtgaatg aactttctgg ccctagatag ctgacgttca tatgcttttg
     3961 atcctttcta taatggtatc gatgatcttt tctggtgttt gattggtcgt gttgattcga
     4021 tgtgtcgcac tttcttcata ccaagaaagt cgttgtgcca ataatgcttt tatttcttct
     4081 cttgtttttc caagagctaa aggtcgaata gacgtttcat cttgttggat acgatcaact
     4141 agacagtccg cttctgcctc taagtaaaga acaatcggct gtgtttttaa aaaacgccga
     4201 ttttctgcac tttggacaat ccctccacca gtcgcgataa ttttatcttc atttgacagc
     4261 tttcttaaaa tatctgactc cacttttcga aagctttttt caccgtaata gtgaaaatat
     4321 tcggcaatcg acaattggat atgttcctca atctttgtat ctaaatctac atactctttt
     4381 tgtaaacgtt gagctaaccc tttaccgatt gttgtttttc cagcgcccat gaaaccaatc
     4441 agcaaaatgc tagccatgag cttctcctcc tgacacaagt tctgctaaat cgtcaaaaaa
     4501 tgccggataa gagattgaaa cagcttcagc tccttctagt tcgcaaggtc catcagctat
     4561 caaagcagct acttgcagca tcataccgat tcgatgatcg cctctgctgg acacttttgc
     4621 gccatgcaga ggtgtcggcc catggatgat cagaccgtct gctgtcgctt ttatctttgc
     4681 tcccattttc tgtaattctt ctgccaccgc atcaattctg ttggtttctt ttacttttaa
     4741 ttcttctgcg tctttgatga tcgtgattcc ttctgcttga gtagccacta aagctagaat
     4801 gggcaattca tcaatcagac gtggaatgat ttctccacca atcgttgctt tcttcaaatg
     4861 actcgtcttg acacttaaat cagccgattg gttctgttca ttgtgattcg tctgggtaat
     4921 cgctgcgccc atctcctcca gcacatctaa gataccggtt cttgttggat tgaccccgac
     4981 attttttaat aacagttggc tttccggaac aagaagccct gctgctagaa agaaagcagc
     5041 cgatgaaata tcgcctggaa cagttatctg ttgaccagtt aatttttgtg gtcctgttac
     5101 cgtgattgtt ttaccttcta ctgagatcct tccgccaaat tgacgaatca tttcttccgt
     5161 atggttacgg gaacgttctt tttcaacgat tacggatgtg ccttctgctt gtaatgccgc
     5221 aaacagcaaa gcagatttga cttgcgcact ggctacaggc atattgtatc ggataggtga
     5281 taaagagttg gttcctgtca cagtgattgg tagatactgc tcattctctt ttccttgcaa
     5341 gtcagcgccc atctcgcgta aaggttccat cactcgcccc attggacgtt tagacaacga
     5401 agcatccccg aacagagtag tcataaaagg ctgtccagcc aaaataccca tcaataaacg
     5461 tatggtcgta ccggagtttc ccatgtcgat tgattcggtt ggttggacaa atgagtggct
     5521 accgcgacca tgaatcttga tcatctcttc ctcttcttcg atttcaacgc ctaattgctg
     5581 gaaggcatgt aaagtactca gacaatcttc tgcgcgcaaa aaattttgta tagtcgtcgt
     5641 tccttccgca attgccccaa acatgatgct gcgatgagaa attgatttgt ctgctggtat
     5701 cctaacagtt cctcttaatc catgtatttg ttgtaataat tgcatttatc cagcctccta
     5761 tttgatccag caatgataac tggtattaga cataatgatt ctttttgctt gtaacaaatc
     5821 ctgttggttt ttaaaactga tttgcagtat tcctaaaata tcttccctgg tctcttggat
     5881 cttcaaatta atcaaagaaa ctcccgcttt tccgatcagt ccagttactt ctgcaataac
     5941 gccaggttca tctggcacat cgataaataa atcaaagaag gcaggaatcg cgccttgctt
     6001 atggataggc aactggtctc gactttcttt tgcccgattg aaaaaagaaa agatttccga
     6061 ttggttctca gtcatgatcc attctgctat ttgtgccatt tcttgctgcc aatccgccaa
     6121 tcgttccaat aatatctgct tattgctcaa caaaatatct gtccacataa caggatccga
     6181 ggaagcgatc ctagtgatat ctcgaaagcc tcctgctgcc agttgtttgg ctcgcggata
     6241 ctcttgattg aattgttctg cctgattgac taagccagct gccagaatat gtggaaaatg
     6301 acttagcatt cctgttatac ggtcatgttc tcttgctgta agagttacgt attttgcttt
     6361 ggttccgcaa aatagttctt gcagtttttc aacatctttt ttcgtctttt ccaaaggtgt
     6421 aaagatgtaa taggcatttt caaatagttt atcatctgct gcctgtatac ctgatttatg
     6481 agagccagcc attggatgtc caccgacaaa ccgaagattt ttctgctcag cggatgtcac
     6541 gacttgctgc ttcgtactgc tgacatccgt aatgatgacg tgatcagaca agttgattct
     6601 ttcaaggaca gcaagataag ctaaggttgt acggacggga acggctaata agataacgtc
     6661 tgcttgttct gcacctgttt caaaatcatt tgcaacgtaa tcaacgatct gctgttctat
     6721 tgcttgctca cgggtttttg actgactatc ccaaccaact actatcagtt ctggattggt
     6781 ctgacgaata caaagtgcca tagaagcacc aatcagtcca agccccacga taaaaacatg
     6841 ttgtgccatc ttatttgccc cctttttaat aatgtttcaa gtagtcacgg tactccttca
     6901 ctgcaacttt caactcaaca agcgaatcac ttgggaattt tgacaatagc tctgtcgcta
     6961 ccacagtggc tacaacattt tccgcaacga cgctagctgc aggaactgct gtactatccg
     7021 aacgttctac acttgctttg taaggttctt ttgtatcgat atcaacactt tgaagtggct
     7081 tgtacaatgt agggatgggt ttcatgaccc cgcgaatgac aagtggttcg ccattggtca
     7141 ttccgccttc gaagccgcct agattattgg tacgtcggaa atagcctctt tcttcttccc
     7201 ataggatctc atccatcact tctgagcctg gtaatgaacc agaagtgaat ccgaccccaa
     7261 attctacgcc tttaaaagca ttgatactaa ccacagcttg tgcgattttg gcatctagct
     7321 tcgtgtccca ttgtacgtag cttcctaatc cagctggaac acctccaacg agcacttcta
     7381 caattcctcc gatcgtatct ccatttttct ttgtctgatc gatcaaatct tttattcttt
     7441 gctcaactgc tggatcaaca actcgtactt ctgacgcttc agaaatttct ttgatttctt
     7501 gaatcgagat atttccaggt atttctgctt ggattccacc aagacaaatc acgtgtcctg
     7561 ctacgatgat gtcaagttct tccagaattt tcttggcaat tgcgccgatt gctacacgca
     7621 tggtcgtctc tcttgcagag gaccgttcaa gcacattccg aagatctcga tgatggtatt
     7681 tcataccgcc taccaaatcc gcgtgtcctg gacgcggacg tgcaactcgc cgcagtgatt
     7741 ggtctttttc aggcacttct gctacagaca tgacagttgt ccaatttttc caatctttat
     7801 tttctaccac taaagtgaca ggtgaaccaa ttgttttgcc gtgacggatg ccagacgtga
     7861 tccgaacacg atctttttcg atcttcatcc ttcctcctct gccgtaacca gcttgtctgc
     7921 gagcgagttc gtgattgatt tcttcttctg acagaggtaa tcctgctggt agtccttcaa
     7981 taatcgctgt taattctggt ccatgggatt ctcccgcagt catataacgc atcttacagt
     8041 cctcctaagt aatctttgaa tgtctctgtg ggaacaggaa cgatttttgc ttgcccaatc
     8101 ttttctagca agatgatatt caattggctt cctcttgctt tcttatcatg ggttatcgcg
     8161 tggaataatt catctggatg acttagatca tacgtaatcg gcagatggaa tttttgaagt
     8221 atcctttcta aagcagctgt cgatccagtt ggagacaagc cgattttttc tgcatgagaa
     8281 gtgatggcta ccattccaat agctactcct tctccatgac tgactacccc atatcctgct
     8341 gtattttcaa ttgcatggcc aattgtatga ccaaaattta aaataagtcg atttccttgg
     8401 tcaaagacat cttcttccac tacttttcgt ttgatttcca acgtagcagt aatgattgga
     8461 acagcatgtt ccagaagatt tttttcgtct ttcagctcgt tcagctgttt ccaaagcgtt
     8521 gcatctgcta tagctgctga tttgatgatt tcagcgattc cttctcgtaa acgacgtacc
     8581 ggcaaagttt ttagaacctc aggatcaatc aatacaccag ctggctggct aaatgttccg
     8641 acaaggtttt tcgctttgtt tgtattgact gctgtcttcc cgccaatact agaatctact
     8701 tgtgcaagta gcgtagtcgg aatttgaacg aaatggatcc cacgcatgaa cgtcgaggcc
     8761 acgaatgcgg ctagatcacc caccattcct ccaccaagtg caagtatccc atcgctgcgg
     8821 gtaaaatcat tatctgctaa aaaatcgtat aactcagctg cttgttctaa agatttactg
     8881 gtctcacccg ccggtacgat tgaaaggact ggttcaaaat tagcttgtct caattggaac
     8941 atgatttttt ctgcatatag tggagcaacg tttgtatcag tgatcacagc tactttttga
     9001 ggtctccaaa tcgtttgaag ccatgatcca gcctgttcta gtgcgccttt ttcaatatga
     9061 atatcgtatt tttggttatc aacatgtaca gtaagcattc atttcacttc tttcttttaa
     9121 aaagctggaa ttaatcgagt gtctgaatct tttccattgc ttctttcatg atcgcaactt
     9181 ctttcatcat gcgtgagtaa gtcttttcat tcaacgattg gggaccgtct gaccaagcat
     9241 ttgcaggatc aggatggatc tctacgatca tgccatctgc tccgcttgcc acacccgcac
     9301 gagccattgg tggtacaagt tcccaaattc ctgtaccatg acttggatca acaatcacag
     9361 ggaaatgagt taattttttc atcaatggga cagcacttaa atcaaacgta ttacgtgtcg
     9421 ctgtttcgta agtccgaatc ccacgttcga taaaaatgac aggagatttg tcttcgactg
     9481 caatgtattc tgcagcatta agccattcat tgatcgtacc ggaaattcca cgtttcaatc
     9541 caacaggttt acctgtttta ccgacagcag atagcagttt aaaattttgc atattacgtg
     9601 cgccaatctg taggatatcg ctgtactcag ccaccatgtc gatatgccct tcatccatca
     9661 cttcagtaat cactttcatt ccaaattcat cagccgcttg acggatgtat ttcaatcctt
     9721 cttcttctaa accttggaaa gcatacggag aagtacgtgg tttgaaggca ccacctcgca
     9781 gaatttttgc gcccccggct ttggccattc gcgcacattc tctgatttgg tctaatcctt
     9841 cgatagaaca aggaccagcc atcgtcacga aactgccgtc accaattttt actccgtcta
     9901 catccacgac tgtattgcca ggatggaatt ctcgagaagt caatttgtat gtgtttaaaa
     9961 tacgcacagc attttctact ccttcatagc gtgtaaatgc tccttcttgt aaacttcgcg
    10021 tatcaccttt tagtccaatc actactcgtt ctgttccttg atcgattgag actttgaatc
    10081 cttctttttc gatatgatcg acgacttttt ttatttgttc ttccgttgct tcttttttca
    10141 taattactat cattgttttt cccgctttct tttgtttact tatctatctt ggctgatttt
    10201 ctctactttt tcaaggtggt tttacattaa ttcctactaa atgaccgtgt cctgttgttc
    10261 tcattttcgc tccttctttc ctacttatga cccagataat caacaaaaaa agatgtccct
    10321 gtaggaaatt gcctctacag tgaacatctt caacgagcct taaaaagcgc tgcaaaaata
    10381 taaaatagcc actatagatg aatttctcta cgtggctgta agtcgtgttt aaaaatcttt
    10441 agccatcata gatacaaacc tggtttgttt gtacccacga agtcatgaat acaaacctat
    10501 ctaaaatagc taaagtaata cgtattcagt tttgtaagtt ttctagattt cataaaaaat
    10561 cgctccaaac tgatgtaaga attgtaccta gtatagacat aaaaaaatcg actgtcaatc
    10621 atttttatga aacttttttt aattttctga tagttaactt tgtttttcag aaaaataaca
    10681 attggtttta ttgaagaaaa tagtcatgaa caaaagtatc tgagaacttt tttactctct
    10741 tgttcatgac atatagaata aagcagcttt gtcttgtaaa ctccgttttt caaataataa
    10801 tctaaagatt gctactgaat aataaagccg atttcatggt cctcttcgct gctgattcgg
    10861 taaggtgact caacatccgc tccccagcta ttgatacccc cgactcctct tacttttgca
    10921 aaaatcgtaa gaaccgttcg tctttctggc ggtagttctt ctatatgtgt tgcctgttcc
    10981 agttcttctg ctttgaatgg taaagcggaa aaagcaaatg gcgtatcctg ctgttcaaat
    11041 cgcaacgaaa aatcttcttg atctttatcc gtattatcca acgtttgatt tcttgttagt
    11101 tctacccatt tcgtttccat atgcatgccg cattcttggg gaacaaggta tggggaaacg
    11161 gggagtcctg ccactttata aataccttcg acaccaccag ccatccgatc cggatatgtt
    11221 tcccctgata ggccttcata ctcaaaaaat tttgctttcg ttggcataat gaaccttaaa
    11281 cccaatgcgg gtaattccgg taatcctttt tgtccataat aatgcatatt gacaagcatt
    11341 ttaccctctc cagcgatttg gtaggaaatt gtcacttggg tttttggtac tgtgttgatt
    11401 tccagtgtaa aaatgatttc aaccgtttca gcgtattcat tacttgaata ttgattattt
    11461 actggagcaa tcggcaaatc gatcttttta ttgtccacca gtacttctat ttctttgact
    11521 tttggataca atccggctcc taaccacata ccggatttaa atccaaatcc gctacctctg
    11581 tcattatcgg tcgttgctcg ccaaaaaact ggaagtgttt cccggtatag ccattctttt
    11641 ccgtttttat tgagcgattc cagtccgcct cttgtataag aaaaaatata atgcgtattc
    11701 cccgatttga cacctaatgt acagtcgccg aagacgattg caactttttc cgtatccata
    11761 tgacttttat ttactgtatt tttcataata atatcttacc tcctgcatgg cgggtttttc
    11821 ttgtccatct gcaaaaacga tcccattccc tgagaattca tagtcacttg gtttatcgtc
    11881 aaagtcaccg ccatagcgca tcacttctct gcctgtgatt ggatcagtca cttggatcgc
    11941 ctgatcttta aaatcccata tatagcctcc ttggtacatt tcataacgat caagcaacgt
    12001 catgtaagaa tccattccac ctaacgaatt ccccatgtca tgcatatatt cacataagat
    12061 gaacggtttt tccgctttat tttcaaggta gtcaatgatc tcttctggtg gcgcatacat
    12121 gcagcttttt acatctgaaa tcgaatcttc gaatgctgga tttggaaaat tcccttcata
    12181 atggacaagt cgatcggggt ctactttttt gaagtaatcg ttcatttgtc gaatgttttc
    12241 ttccgcataa gattcatttc caagagacca aaaaagaata cttggatggt tttttaatgt
    12301 ttcgaaattc gtttttgctc gatcaagaac agccgcctgc cactgaggta ctgcacctgg
    12361 tacgttgtaa gaaggctcaa atgcgcccat tttttgccat gagccatggg attcgaggtt
    12421 cgtttccgcc atcacatgga ttcctgcttg gtcgcataaa taataccaag tgatctggtt
    12481 ggggtaatga gatgtccgta cgccgttgat ctttgcattt tttatgatag ccaaatcttt
    12541 ttccatatct tctatagtga tggcgcgtcc ttttttcgga tgccactcat gtcgatttac
    12601 cccgttgata atcatccgtt ttccattgaa gtagatgatt ttatctttga tttcgatttt
    12661 acgaaaaccg aaggaatagt caatatactc cgttactctt ttttcttcgt ctatcgtctc
    12721 caattcaagc ttatataaat tgggctgaaa atgcccccac agtgccacat tttcaaatcc
    12781 atcgatgcga atcgtcattt gctcttttaa cggatattgc tgttgataga tttccttccc
    12841 atcaggatct agtatcgcta cattcagctg actgtttttg gctacttgct caaaagacaa
    12901 atctacttca agtttgccat tttgacattt gtcatctaag cgaggtttga tccaaagatc
    12961 ttctaaatgg ttttgtcctc gagcaactaa gaagacatct ctgaagatcc caaaaaatcg
    13021 gaaaaagtcc tgatcttcca agaatgcagc tgtacttctt ttgtaaacct ccacagctag
    13081 aatattttct tcttccaaat aaggtgttaa atcaaattca gacggagtga acgaatcttc
    13141 ggcatacccg ataaaatgac cattcaacca aataaacatt gcttgttcta caccagcaaa
    13201 atgaatcgag atttgttttc cctttagacc ttcttctaat cgaaaagtcg tcaaatacga
    13261 accaaccgga ttgtctttag cagctgaaaa gatcccttcc gcctctttct cagggcttgt
    13321 cgtgaaaggg ggacggcgaa atacatgtcc ctcccacgga tacatcgtat tgatgtaatg
    13381 gatcttgtca taatttgcta attcaatatg actaggaaca gtgatttcat caaaagaaca
    13441 ggcatcaaaa tcttttttat aaaatgtttt tggtcgtacc gaaggatttg gcgaataaca
    13501 aaaacgccat gatccattta aagactgttt aagggaggat gtggtttcta cttcttccct
    13561 tgttgcataa caaacatgat cgctatgtgc atttgattgg ttcactctaa aaacttgtgg
    13621 atcatctaac cattttatat ttgctttcat acttttttcc ttctctctca taactttttg
    13681 aaatgaagca gttctgtttg gaaatcacct tgaaaatcgg ggaagtaaag tccaactgtc
    13741 atcaattcgg cccccgaaaa tatccgcttc gaggtctgtt cctgataaag tgcctcatca
    13801 tctaacccca tcagatatac ttcatggaac gctggttgag ctgaagctag tatcctccct
    13861 aaaaacacaa tagcttcatc ttgtttagga gacacaaaca tccatgcggc aatattttct
    13921 tcaaaaggac tcgccaaacg ataataatgg ccatactgga tcaccggacg aatcgtttga
    13981 taggatataa tttggtcttt tacttgcttt ttttcttcag gagacagctt tgtcaaatcc
    14041 agttcataac caaaaacagc gctcatagct gtttctcctc ttgttttcaa cgaagtagat
    14101 ctgtcggtct gatgatttgg tacagctgat acatgagctg tgatggacga aatcggataa
    14161 gcaaggcttg ttccatattg gattttcata cgctccactg catccgtgtt atcacttgtc
    14221 catgactgcg gcatataata aataaacccg gcatcaaacc gtccacctcc accagaacag
    14281 ccttcccata agatttccgg ataatcagtt gtcaaatgtt caagtagttc ataaagcccg
    14341 agcatataac gatgcgctac ttctccttgt ctctgtgggt caagggtaat cgaatagaca
    14401 tctgaaagcg aacggttcat atcccatttg atgtagtcaa gaggaatcgt atctaaaatc
    14461 tttctcattt gtttctcaat cgtctgccga acatctaacc ttgtaaaatc cagtacatgt
    14521 tgtgatctgg aagcagaagg cattctacct ggctcctgca taagaaaatc aggatgtgtt
    14581 cgatacagtt cagaatcaac cgagatcatt tcaggttcaa accataaacc aaatttcaat
    14641 cctttgctat gagcataatc tgctatttca cgtaatccat ttgtcaactt gccttcatac
    14701 tcgaaccagt ctcctaatga agaattgtcc gcatctcttt ttccaaacca gccatcatct
    14761 aatacgaaca tttcgatgcc tagctccttt gcttcatcaa tgatcgcctt gattttttca
    14821 ctattgaagt caaagtaagt ggcttcccag ttattgacca gaatcggccg ttcagcaaat
    14881 tgatgacgac ctcttgcgat tctttcacgt agcaactcat ggtggatctg actcattttg
    14941 ttcaatccct gacttgagta agaaaggatc atttcaggtg tttgaaaaga ctggcctgca
    15001 ttcaattccc acgaaaaacg atggctgttg atcccaccga cgactctcaa ttgattgatc
    15061 tgatcttttt caagttcgaa agaatggttg ccggaataaa caaattgaag tccgatcgct
    15121 tctccagtat tttctgtcgt atgatgatga actaaagcaa taaagttatt catatggtga
    15181 ctgctcgttc ctcgacggct ttcaaatact tttgttccag ataaaacaga ttgccgtgaa
    15241 atctgtcttt cattgacatg tgctcctggc aaagcaatga cttcatcaaa tcgcctagta
    15301 tgggcaaagt ctagctggaa actagccgct ttttctagtt tgattgtttc tccggtttca
    15361 tttcttatct ttacagatcg tgtgatcact gctctatcag taaatatggt atagaacaaa
    15421 tcgaaataaa tcgctgtttc tctatcctta agtgaaatca ccaacgtttg tgcctctgac
    15481 ttgtccatca cataagtttg aggcaagcct tcgatctctg gcttcccatc tgtaatataa
    15541 tggctgtcat atctcagatc cagtaagtct gatccattct tgcgctgaat gatcgatgcg
    15601 ggcgttcgaa agtccatgct gccatgagaa gagtattctt gcggtagtgt atctttcgac
    15661 agagaccggt caggatttaa gggaacattt cctgaaaatc cacgatctct tcggggataa
    15721 tttttattgt ctttgtactg cttgactggt ttgccaaaat aaatatgcgc caatacattg
    15781 ctttcttcta tctttatcag ataagaaaga aatgtatttc gtaaatggaa gactcggctc
    15841 ttttcatcaa ataagatgaa atttttatct gtttttctat gcgtaatatc catcatttca
    15901 ctcccaacaa aacggaaacc gtttactttt tttataattc ctgtatactg tttactaaac
    15961 aattttgtca ataaaaattt tttatttttt tgaggagtgt gtaaaaatgg ttggaattag
    16021 agatgtcgca cgaaaggcaa atgtctctcc tgctacagtt tcccgtgtgt tgaataacga
    16081 tcagacgatc catgtggctc agactactcg gcaacggata gtagaggcag ctgctgcgtt
    16141 ggattatgac atcagtactc gtaaatatat caaaaagaga atgccttcga ttggtgtgat
    16201 ctctacgatc ggcaaagcga gcgaagaaca ggatatctat tacaaacatt tgcgagtagg
    16261 gatggaagaa gaagctcgac gtctgcattt agggatgaat cgaatctata atctatctga
    16321 taatcctaaa caatgggaag atctagatca gttaggtgcg gtgatcgttg ttgggactgt
    16381 tacgaaagaa tcgattaccg ccttgttaaa acaaaacatg caaatagtcg tcgtggataa
    16441 ccctgacatc cagcaagagg tcgatatggt gtatgcagat cttgaacgga tgactaaaac
    16501 cgtactggaa ttgttcttag aaaatggtca taaaagaata gcctatatcg ggggatataa
    16561 agtcgatatg gatgaattcg gtgggaagca gatttctata aatgaaaaga ggatgcgtgc
    16621 ctatcagcat tttatgatcg atcatcactt agaaaaatat atgtcttatc atctaggtgg
    16681 ctggacagaa aaacaaggtg aggtacttgc gactaatcta ttagaaaatg aatcccctct
    16741 tcctactgcc attcttgtcg ggagcgatcc aatggcaatt ggtgtctatc gggcattgaa
    16801 acaaaaaaat atccaaattg gagaagagat tgttgtagca agctttgata atatcgagac
    16861 aatcgcaaac ctctcccctg gcttgaccag tgttcaaatc aatgcgaaat caattgggca
    16921 agcagctgtt cgaatggcat tggagcgaat tgacggtata cgggacgaac caatcatttt
    16981 gacttatcct gcacgattga ttgttaggga aagttttatc ccaagaacaa aaaggtattg
    17041 aaagagaggt tgtgaaaagc cgcttcgttt cttcatactc aaaacgaaac ggctttttca
    17101 caacctctta cttatactta ttcacaaaat ccaatgtctg ctggtaacaa ttttgcgatt
    17161 cttttagcgt gtaatcaaat tggtattcat gggtaatctc tttttttgta tctgtataaa
    17221 acaaggattg cacgggtacc tttaattctg tcaaccgatt ttctaatgct tgaccttgct
    17281 cctggaatga gtatgcattt ccgtcagtta tatacgttgg cggaaattcc ttagaaacat
    17341 ggtcagccac actagcttct ttcaattgct cactcgtttt ccaatctttt gttccaatca
    17401 acgaccacgc aacggttttg acgaaaaact tcataaatcg atcatccgaa tgctgctgtg
    17461 ctgttagctt caaatcaaca ggtccgcaat aagaaatggt tcctttcaga ctatcttttg
    17521 gaagtagctg tttgattttc atttgtttag catacgcatc attcgtttgt atagctgcat
    17581 attgcaatgc gatttgtgaa cctgcactat ctccaccaaa aaataattgt tccatgtcta
    17641 gcaatttgtc gtctttttct tcagctaata aatctttgat cagttcattc acttgaagaa
    17701 cttgattagg gtattctgaa tcaggcgcta attcataatt catcgcaacc actgcgatgt
    17761 tggcgtctga aactaatttt gtcgcaaatt ctttgacacc cgatttatct ccgccgacga
    17821 atcctccacc atgtacccaa aatagaacag gaacaggccc cttgctgttt tttggatagt
    17881 agatgtcata agtattctct ttatacttgg actcatacgt caaatctgtt tttttcgcca
    17941 cgtttttttc tgcttgatcg aacgtttttt tatcagtgat ttgaacagta tttgaaaaca
    18001 tatggttgat caagaatgct ccaggtctgg gggaaagctg gaaggcaaga aagattgcaa
    18061 acgcgatccc aagaatgatt ccgaagaacc atccgataaa tttaagaatt ttctttttca
    18121 taaggctccc tcagtagtta gtattaagca ttctgattat accatagtgt tatttcctat
    18181 caagctctcc tagatgattt aatgattttc cccaaacttc ttctgcaatg cttttgacaa
    18241 aagaaatttt ttccattgtt cttcaaacaa aacattttct tcttctgttg aagcaaatcc
    18301 acattgagga cttaagcaca aacgatccaa aggcagatat cgtgtcgctt cgtagatccg
    18361 gtcaatcact tgctggtgct cttccatttc gccatttttc gttacagtca aaatctaaga
    18421 caactaattt atcttcagag actttctcta aaagaacaaa actcccagca cgttcactat
    18481 cgtattctaa ataataggca cggacatttt cttgagtaaa ataatgggtc tgctacgtcg
    18541 tcatcccccc tgatgccgcc aaaatcgaat aataatttcc gcgtcaaaca tgtatattta
    18601 gtgttgaatc atttgagaat atgaaaataa aaatcttttt gatcaatcag ttaatcaaat
    18661 tgaaataacg aaaaatctga gacagaatga cgttcattct gacccagatt tgattttgca
    18721 caactcaaca gtccttattg gcaaatcgcc aaaataatgt agactaacta aaaagactaa
    18781 gaaacaataa caaaagttaa cacttcttag tctttcttta gcacgtctat ttattaaaga
    18841 ttttagggcc ttttctcatg tttttacgat cattcttagg tgtttccatt tgtttggatc
    18901 ggcctccacc ttgtttcttt ttgatcaatt ctttcatttt ttctttttgg tccattttta
    18961 ttctccttta acgaatgaag cgactaattc gatatgatgg gtttgcggga aaagatctac
    19021 aggctgaact ttcttcacgg cataacctgc ttctgcaaat aacttagcat ctcttgcaaa
    19081 agttgccgga ttacatgaga tatagacgat tcgttctggt gacatttcga ttgctgattc
    19141 aataaaatga tgatctaatc cttttcgtgg agggtcaact acgatcacag atggtttgat
    19201 ccctttttct tgccattttt tcataacttt ttctgctttt ccgacttcat aatgaacatt
    19261 ttcgatttta ttatttaaag cattgaactg tgcatcttca attgcttctg gaataacttc
    19321 catcccatac accttatcca ctctgtctgc taaagacaag ccaatcgtac ctattcctga
    19381 ataagcgtcg atcacaatgt cttttttatc taactcggca aactcgatgg ctgtttggta
    19441 cagatactct gcttgttgag tatttacttg ataaaatgac tttgctgaga ttcgataggt
    19501 ttttcctaat agctgatctt cgatatagtc tctgccatat aaaacttttt ctttgtctcc
    19561 taaaatcaca tttgtttttt cttcattgat attttggatg accgaaacca cttctggcaa
    19621 ttcttcatga atagcttcag caatttcctt ggatttaaag aatttttctt tacgagtaac
    19681 taaaacgacc atcatttcat gggaataatg accacgacgg ataatgatgt gacgaagctg
    19741 tccttcattt ttttcttcat tatatcctcg tacttggaac cgttgcaaga tagctctgac
    19801 tactttgatt gcttgatcga tgacaggatc ttggatataa aaatcttcga tagggactag
    19861 atcatggcta ttctttttgt aaaaaccggt ggctaactgt ccatcgatac ggcgaacagg
    19921 gatctgtgct ttattacggt agccgaatgg ttctgccatt ccgaccgttg gaagtacagg
    19981 aacttctggc atcttagcaa tcttcgccat tacatgctct acttgttttt gtttgaagac
    20041 aagctgttgt tcatacttca aatgggctaa aggtgcgata cctatgcgaa gtaatgcatt
    20101 gttttctaca ggctgacgat caggtgattg tgtaataaat gtttcgactt ttccatatcc
    20161 aaacttattg cctgctttta ctactagtat ttctacttct tctccaggga gtgcattttc
    20221 gataaataat ggataaccat cgatttttgc tacccccatt ccttcataac tcaagtcgac
    20281 gatttcgact gtatatcgtt cgtttttctt tactatttgc tgttctgtca tattttgctc
    20341 ctttaagcct atttttcaat cctactgaac tctactataa atcagctaat tacacaacta
    20401 aaaccggcac tgaccttact acgttcagca ccggcttttg attaattctt ttgattaggt
    20461 gtttcgtctg ctatttttcc gtcaccgtcg atatcttcat ccgttaaacg ttcgacttct
    20521 tttacgaatt ctttgctgac aacctctaat tcttcttcat ctgtacctgt gattgcttca
    20581 tcaggaatct gatcggtatt ggcaaagaac tcaatgtgtt gatgaagatt ttggaaatgc
    20641 atcggtgctt ccccgccgta ttcaccatct aagttgatca ttactggtcg attgatatca
    20701 tcttctgttt caacgaacaa actgcttgtt ttggtataga ttattcttgg atcatcgaca
    20761 tgtcgaccgc cattcagcat gagtgctgca atatgcaaga tctcaaagat atttgctgtc
    20821 ttcacaatga ttaaagaaaa cttcccatca tccaatttgg catctggcgc gatctgttcg
    20881 aatcctccaa cagagttcgt cagccctagg aaaaacatcg aagcatttcc gatatattcc
    20941 ccttcatcat acttcaagcg cattttgatt ggctttactc gagggagcat ctctgctcct
    21001 ttcgcaagat aagcaagata accaaaaata ctctttagtt ctgatggaac ttcataagtc
    21061 aattctgtca aatagccacc tgcagcgata ttgatgaagt aactgttatc agaaaggccg
    21121 atgtccattt ttacggtttg gtgttttttt atcacttctg ctgctgcttt aatattatct
    21181 ctaggaattt ttaatgctcg agcatagtcg ttcgttgtac cagctgggat gatggccatt
    21241 ttaggacggt gttcaagcgg agcgattcca ttaaccactt cgttgattgt gccatcgcca
    21301 ccagctgcca ctactagatc aaaacctagt tctgcgactc tacatgcttc attcttggct
    21361 gaatctggtt ctggtgtagt tgcataggcg ctggcttcaa atcctgcttc ttctaatgcc
    21421 tgtaggatat ccgccaaatt ttttttcaat aattcttttc ccgaggtggg gttataaatt
    21481 acgcgtgctt ttttcatttt cagcctctat cgtttcgcta attcatcttt aagtagctta
    21541 ttcacgacac caggatttgc ttgtccttta gttgctttca tgatttgacc gactaggaag
    21601 ccaaccgcac gatcttttcc atttttgaaa tcatcgacag attgttggtt gttatccaaa
    21661 atttcattaa tgattggcag tagtttgttc ggatcagata attgcaccca accatttttt
    21721 tcaactactt cttgggcttc tccgccattt gtgatcaatt cacggaagac ttttttggcg
    21781 atttttgagc tgatcgttcc atcggcgatc aaacgaatca ttcctgctaa gttctcaggt
    21841 gtcaattttg tctcagctaa ttccagtttc tcactgttca agtaagcaga aacttcaccc
    21901 atcaaccagt tagatgcttg cttggcatct gcaccatttt ccaaaacagc ttcaaaaaaa
    21961 tcagacattt cttttgtaag tgtcaatacc attgcgtcat actctggaag tccaaactca
    22021 cggatatagc gttcgcgacg agaagctggc atctcaggca aactagcttt tactttttga
    22081 atccattcgt catcgatcac gaatttcggg atatcaggct ctgggaagta acgataatca
    22141 ctagaccctt ctttgacacg catcaaaact gttttattcg ttgtttcgtc aaaacggcgt
    22201 gtttcttgtt ggatttctcc tcctgaaaga agaacttttg cttgacgttt ttcttcaaaa
    22261 gctaaacctt ttttcacgaa agataaagag ttcaagtttt tcaattctgc tttcgtaccg
    22321 aactcttctt gtccataagg gcgaagagaa atattggcat cacagcgcat agagccttct
    22381 tccattttca catcgctgac ttctgtaaat tggataattg atcgcaatgc ttctagataa
    22441 gcgtatgctt cttctggtga acgcatatca gcttcagaaa cgatttcgat caaaggcgtg
    22501 ccttgtcggt tcaaatcaac gtaagaataa ccgccaatac catgcatgtt ttttccagca
    22561 tcttcttcta aatggacacg ttcgatccgg atttttttct ttttaccttc tacttcgatt
    22621 tcgatccatc cgtcatgacc gattggttga tcaaattggg aaatttgata tgcttttgga
    22681 ttatctgggt aaaagtaatt tttccggtca aaatgagtat ctttagagat ttcacagttt
    22741 aatgccaaag ctgctttcat cccgaattcc agcgcagctt tattcattac tggcagcacg
    22801 cctggataac cccagtcgat cacgtttgta ttgctgtttg gttctgcacc aaaatgagct
    22861 ggcgctggag agaaaatttt tgagttggtt tttaactcga cgtggacttc tagtccaatg
    22921 actgtttcaa aattcattcg tctttccctc ctaaaatagc tggttgttgt ttatggaatt
    22981 ctgttgcttg ttcaaacgca tatgccgctt gatacatcgt tttttcatca aaataattac
    23041 cgatgatctg caatccaact ggtaatcctt ctgataaacc tgccggtaca gacatacctg
    23101 gaagtccagc caagttgaca gggatcgtta agatgtcact catgtacatt gtgattggat
    23161 cgttaatgtt ctcaccgatt ccgaatgcaa ctgttggtga agatggcccg atgatcaagt
    23221 cataatctgc aaagactttt tcaaaatctc gtttgatcaa tgtacggact tgtcctgctt
    23281 ttttgaagta agcgtcataa taacccgcgc ttaaggagaa tgtacctaac atgatacggc
    23341 gttttacttc ttcaccgaat ccttctgaac gtgagttgac atatacatca tctaatgatt
    23401 tcacattttc agagcgataa ccgtagcgga taccatcgaa tcgttgcaaa ttggaacttg
    23461 cttctgatga ggcaatgata taataaacag ccacaccgta ttttgaatga ggcaagctga
    23521 cttcttccac aacagcacct aatgaacgga atgtttctgc tgctttcaat acagcttctt
    23581 ttacgccagg ggccacacct tcgcctaagt attctttagg taaagcaatc ttcattcctt
    23641 tgatgtcgcc tgtcagtccc tctgcaaaat caggaactga aacgcctgaa gaagtaccgt
    23701 ctttttcgtc ataaccgctg atagctgtca atgctaatgc attatctttc accgtacggg
    23761 tcattggtcc gatttggtca agagatgaag caaaggcaat caaaccaaaa cgtgagacac
    23821 ggccataagt aggtttcatc cctacgatgc cgttaaaagc tgctggttgg cggatacttc
    23881 ccccagtatc actacctaaa gaaactggaa tttgtccagc tgcaactgct gctgcagaac
    23941 caccagaaga gccaccaggt acttttgttg ggtcccaagc attttttgtt ttcttgaaat
    24001 aagaagtttc tgtacttcca cccatagcaa attcatccat gtttagctta ccaacaggga
    24061 tcatatctgc ttgatagatt ttatccatga ccgttgcatc ataaattggt tcaaaattgt
    24121 aaaggatctt agatgcagcg gttgtcaaga tatcttttgt tacgatatta tccttgatac
    24181 caattggaat accagcaagg acgttgcttt catcaatccc tttttcatct aaagcttttg
    24241 cctgttccaa tgctttttct tcgttcaatg taatgaatgc ctcgacctca ggctctgttt
    24301 ctttgattcg ttcaaatgtt gtttttgtca aatcttgtac agtgatttct ttcgatacaa
    24361 gcatatgatg cagttcttct agagaacgat tgtgtaattc tgtcattatg cgccagcctc
    24421 cccattatcg atgattgcgg gtactttgat aaagccgtct tctgtttccg gcacattttt
    24481 caataattca tcgcggttcc atccagcttg cgcccgatct tgtcgcatca cgtttactgt
    24541 ttcaaccaca ttcgacgtaa atggaacacc ttccgtatct acttcttcca gcatttcaac
    24601 catatcgatg attttaccta attggtcggt aaaacttgca agttcttcat ctgcgaacgc
    24661 aagttttgct aatttggcaa cgtgtttcac ttgttcttca ctaatcgcca tactacccct
    24721 tctttctaaa cgagttaaaa actttgctct ctattttaac acaagagtgc cgtgtctgaa
    24781 attgtttatt gaaatatgtc tgaaaaaact catttttcat tcttctactg agcgactttt
    24841 agccaatgca tctgccattt gatcttctgt ccaaacttcg attcctaatt cttgtgcttt
    24901 ggtcagcttg ctgccagcat cttctcctgc taccacgata tccgtttttt tcgatacgct
    24961 accagtcact ttcccgccaa gattttcgat cgtctctttt gcttcttcac gagtaaatcg
    25021 tgttagttta ccagtaagga ctactgtttt ttctttaaaa ggagactcga cttcttgcaa
    25081 ttgggcattt cgaagccctt tgtattcgaa attgacgcct gcttgtttta attcattcat
    25141 caattcatgg acttcttcgt tactgaaata agtgactaca ctgtcggcaa tgatatcacc
    25201 gatcgtatca agcgcaataa tcgactcata gtcactttta ctcaatgttt ctagatcacc
    25261 gaaatgttct gctaaaattt ttgctgcttt cgctccgaca tgtcggattc ctaacccgaa
    25321 aatcaaccgc tcgaccgaat tatctttgct gttgtcgatt gctgtcaaaa tattatttgc
    25381 tgatttttct tttattttgt ccaacgtcaa taactcttct tctgtcaatt tgtaaagatc
    25441 agcaacatct gctactagtt ttttatcata catctgttct agtacgcgcg gccccaaccc
    25501 atcgatattc atcgcatttc ttgaaacaaa gtggttcaaa ccttctttca tttgagcagg
    25561 gcatttagga ttgatgcacc gtaaggctac ttcttcatcc aaatggacaa gttcacttcc
    25621 acaaactgga caatgtgtag gcaattggta ttcttcgctg tctttaggtc gtttatctaa
    25681 cacgacttgc gaaacttctg ggatgatgtc tcccgcttta taaatcagaa ctgtatcttt
    25741 caagcggata tccttcaact taatataatc accgttatgc aggctggcac ggcttactgt
    25801 cgttcctgct actcggactg gtgtcatgat agctgttggc gttactaccc ctgttcgacc
    25861 gactgtccat tcgatgtttt cgattagcgt ttcaacttct tctggcggaa acttgtaagc
    25921 tgctgcccat ctcggtgctt tgatagtaaa accaagttga tcttgtagcg agaattcatt
    25981 cactttgatg acgattccat cgatttcata gggcaaatcc acacgtttgt catgatattc
    26041 ttcaatgtat gaccacactt catcaatcga atgacataga cgtttttccc gattcgtgtg
    26101 gaatccaatt ttttctagct cttctagcgc atcatactgg gtttttgctt gcataggacc
    26161 gaaatcagct actgtgtata aaaaagtgtc cagatttctt ttcgctgtga ttttagaatc
    26221 taactgacgc aaagaaccag ctgcggcgtt ccgaggattc gcaaagatat cttttccctc
    26281 tgcttctctg tcttggttca attggacaaa tgaccgtttt ggcatgaaac attcgcctct
    26341 tacttcgatg ttcattggtt cttttaattt gattgggacc gaccggacag tttttaggtt
    26401 ttcagtgatg ttttctccta cagttccatc cccgcgagtc gcacctctca cgaaatttcc
    26461 atcttcataa cgcaaagaaa cggaaagtcc atctattttt agttcacagc aatagtcaac
    26521 tacacgtccc actgcttttt gtacacgttg atcaaaagct atcagctctt ctttgctgaa
    26581 aacgtcgttc aaactgtaaa gcggaatatc atgagtgact ttttcaaaac cttctaatac
    26641 ttttccgcct actcgttgag tgggagaatc agacgtgatc aaatcaggat attgttcctc
    26701 gattgctact aattctgcgt attctttatc ataaacatag tcttccactg ttggtttatc
    26761 ttctacgtag tattcgcgag accattggtt cagtcttgta cgaagttctt ctgctcgttt
    26821 agcagcttcg tcaatcgtca tttcatgttc catcatttac cctccactac taagcttttt
    26881 caattggcgc aaaggcagcc aaaagacgtt ttactccttg acttgggaaa gccacatcta
    26941 gttctagatc cttcgacgtc ccgcttactc ggacgactgt tcctagaccc cattttttgt
    27001 gtttgacttt atctccaggt ttccagctgt tattttcccc tccagtagca cttttagagg
    27061 gaacaaccgc tgtctctggc tgtttatagc tatatgctgt ttttttagcg aatacaggcg
    27121 tttttttagg cgtaggacgc atgccttcga tttccagtaa ctcctgatcg atttcttcta
    27181 caaaacgaga aggtcggtta tattgcgtcc gtccatataa tgttctggag aaagcatttg
    27241 ttaaatacaa cgcttcttct gctcgcgtga tccccacata tgcgagacgg cgttcttcct
    27301 ccagttcgct ttcttccatc aatgctcgag ataaaggaaa aaccccctct tctagaccaa
    27361 tcaggaatac aactggaaat tctaatcctt tggctgcatg caaagtcatc aatgtcactt
    27421 gggaagcatc ttcttctaaa ttgtcaatgt cggaaacaag tgccagatca tttaagaaga
    27481 ccgttagttt ttcttctggt gcatcagcgt cttcttcatt ttgctgttcg aattgtttgt
    27541 cgaattcttg tgtgaccgtt aaaaattcct ccaagttttc gatgcgtgct tgtgcttcta
    27601 atgtattttg gatttttaag tcttctagat atccgctacg atccaaaacc tctttcgtca
    27661 attctgtcac tgtcaagtat gggatcatct gagtgacttc ttggatcatc tctccaaatg
    27721 cacctaattg ctgtcctgct tttccaccga tatttgccag atccacattt tgcgctgctt
    27781 caagcagtgg ccattcatga agagaagcaa aactgcgtag tttttcgata gatcctggtc
    27841 cgatgcctcg tttgggcgaa ttaacgatac gctcaaaact gattgaatct tgaggattta
    27901 ccagcacatt caaatacgct aaaatatctt tgatttcttt acgatcgtag aacttgtgcc
    27961 ctccaaccat tttataagga atatttgctt tcaacagagt ttcttccatt acacgagatt
    28021 gtgcgttcgt ccggtacaag atggcaaagt cgccgtaatt ccgatgattg cttcgaatct
    28081 cttcttgcat ccggtctacg ataaaacggg tttcgtctcg ttcattatct gcacggtagt
    28141 aagtgatctt gtttccttct ttgttttctg tccacaagtt ttttggtttt cgattgctat
    28201 tgttttcaat gacttggttt gctgcgctaa ggatgttttt cgtggagcga tagttttgtt
    28261 ctagcaaaat tactgcagca tctggataat ctttttcaaa atccaagatg ttctgcatat
    28321 ctgcaccgcg ccagccgtaa atactttgat ctgcatctcc cacgacgcac aggttcctaa
    28381 atctacctgc caataaattg actagcgtat actgtgcgtg atttgtatct tggtactcat
    28441 ctacatgaat ataatggaat ttgttttgat aatacgtgag tgaatcagga tgttcttcaa
    28501 ataatcgaat cgtattcatg atcaggtcgt caaagtccat acattgatta tattgaagct
    28561 ctttttggta agcagcataa cattttgccg caatctcttc gtaataactg ccttgcattt
    28621 ttgcaaaatc ctgaggggtc tgcaggctgt tttttgcttg agagattgtt cctaatatac
    28681 ttcgaggatc atattttttc ggatcaatgt tcagttcttt tagtatccgt ttcatcaagg
    28741 tcagctgttc agaagaatcg atgattgtaa agttccggtt atacccgata aaatcaacat
    28801 ctcttcttaa aatacgcaca cacatcgagt gaaaagtcga tacccagaca tcttcgcctc
    28861 cagatgcgag aatcgcattg acacgttctt tcatttcttt tgcagcctta ttggtaaaag
    28921 taatcgctag gatgttccaa ggattgactt ctttttcctc aatcaaatag gcaatgcggt
    28981 gtgtcagcac tctcgtcttt ccacttcctg cacctgccat tagcagtaat ggtccgtcag
    29041 tgtgaagaac agcttctttc tgacgggggt tcatcccgtt taataattca gctttggatg
    29101 acattaacaa catcctttct tttatcaatc ttgttaatta tagcattttc ttggctttca
    29161 ttaaactagc tagttcaaaa tcatttgttt ctttttatgg atttttcctt gtcaattcgt
    29221 tgatttcgcg ctaaattaaa agaaagcgag gcgtttgaat ggaaaaaaaa ggaaaaatca
    29281 agaaattaga taagactcag atatatttta ttctaaaagg aatatttgtt ggactgctga
    29341 caggaatcgt cgttagcctg tttcgattat ctatcgaaaa gctaagcgat ttagttcgaa
    29401 gtatctatga aatgagtcgg gaacagccga tttacttaat tggcctagct atttgctgca
    29461 tccttgcagc atttttcgtg ggttatctgg tcaaaaatga accggatatc aagggaagcg
    29521 ggattccaca agtagaggga cagcttcgcg gggaattatc aatgaactgg ttctctgttt
    29581 tatggaaaaa gttcattggc ggtgtacttt cagttggtgc gggccttttt ctaggtagag
    29641 aaggaccttc tatacagctt ggcgcatcca ttggacaagg agctggacaa ttgtttcgga
    29701 gtccatcttc agaagaaaag attttaattt ctagcggtgc aagtagcgga cttgcagcag
    29761 cattcaatgc tcctatcgca ggattactct ttgttttaga agaaatccat cactcctttt
    29821 ctccacttgt ttggctgaca tcatttgctt cagcaatcac tgctaacttc gtctctcttt
    29881 actttttcgg cttgagacct gttttatatc taggtgacct tccttctttg ccgttgaaat
    29941 attatggttc cttagtgatc ttaggagtac ttctaggcat cttaggtttt atttatcaga
    30001 atgtcttgct ggctttgcca aaattttaca accgattgcc tttaccagct tattttcacg
    30061 ggttcgttcc tttcttgctg attttgccaa ttggttattt atggcctagt actctaggcg
    30121 gaggcaatca aatcgtttta gcttttggac atgcttcctt gccaatcatt atgattatcg
    30181 gtttgtttgt tcttcgattc gttttttcca tgatttctta cggtgggggt cttccagggg
    30241 ggattttcct tccgatattg acattggggg ctttaatcgg tagcttttat ggaaacatta
    30301 tggttgattt cgggatggat ccaatctaca tcaaggattt tgtcgttatt gccatggctg
    30361 gttatttcac agcgattgga aaagcaccgc tgacagcaat tattttagtg acagaaatgg
    30421 ttggctctct taatcatctg atgcctttag gattggctgc tcttgttgct tatatcgtca
    30481 atgaccttct cggcggtaat ccaatttacg aatcattatt ggaacgtcta cttggaaata
    30541 ctcagaccgc ctcactaaaa ggtgcaagga caacttttga ttttccagta acggcagaga
    30601 gttctttaga cggattaatg gtacgagact tcaattggcc taaaaatatg ctgctaatct
    30661 ctatacgcag aggaaatcag gaaatcttga ctcacggtga tacgatcatg aatgtaggag
    30721 atattttggt tatcttgact gatgaagcaa acttgccaag aatcaagcag gaaatcgagg
    30781 ctaaatcttc tatcacaaat ttgaagcaat cattttaacg aacatggaaa aactatttta
    30841 caacaactat tcatctacac tacttgattc tatatgattc tataaaaaaa gaagaggctg
    30901 ggaaaaccca gcctcttctt ttttatttct atgcttctgc tactgcagga gcttttctca
    30961 ataaaccata aatcacacca gatacgactg ctccgatagc aataaacacc aaatacatga
    31021 ccgggtgact cagtaagaat acaacgaaaa tcccgccgtg aggagccagt aatttgattc
    31081 cgaaagctcc gactaatccg ccagttaaag cagaaccaat aataaaactt ggaatggcac
    31141 gaatcgggtc agctgctgca aatggaatcg atccttccgt gacaaacgat agtcccatca
    31201 cgatgttcgt caaaccagca tcttgatctg ttttgctgaa tttattctta aacaaacgtg
    31261 tagcaacgaa aattgccaat ggaggtacca ttcctccagc cataacagaa gccatcacga
    31321 cacttccgcc agttgcaaca gtcgtagcta aagttgctgt accaaacaca taagcggctt
    31381 tgttgattgg accgcctaaa tctgccgcca tcattccagc taataatgca ccaagtaaag
    31441 cagcattcga accactcata cttgtcaaga aaccgttcaa tgcatcattg attgctttca
    31501 tagggacatt gaccagcaac atcaagaaac cagtaatcag taagccaaac actggataaa
    31561 agaggatcgt tttaattcca tccaatgatt ttggtaagtt cttgaataat ttacgtaaaa
    31621 atacgatgac ataaccagct aagaatccac caatcagtgc acctaagaat cctgcaccgc
    31681 ctgtattagc taatgcacca gcagcaaatc caacgatcaa accagggcgg tcaccgatac
    31741 ttgaagcaat aaatcctgct aataccggca gcatgaaacc aaatgcagct tgtccgattt
    31801 ggttgaacca gctagcagct tgattgtaat tccctagatt tgataattga tcatgtggca
    31861 cgccaataaa ttggtcgatc atgaaagaaa gcgcaatagc gattcctcca ccaataacga
    31921 aaggtaacat gtgtgatacg ccgttcatca aatctttgta aattctttgt ccgatagtac
    31981 catccgcatt tccttcttct gaactgcttt cttttccatc accatggaat actggtgcag
    32041 taccactaag ggctagattg atcaactctt ctggtttacg gataccatca ctgacaggac
    32101 gattgatcag ttcttttccg tcaaagcgat tcatctctac ttttttgtct gctgcaacga
    32161 ttacaccttc tgcacgggcg atgtcttcag cagtcaatcg gtttttgatt ccttctgaac
    32221 cgttcgtttc gactttgatc tccacaccca tttctttcgc ttttttcttc aaagcatctt
    32281 ctgccatgta agtgtgagcg atccctgttg gacaagctgt aacggcaaca acgaattttc
    32341 tttctggatg agcagaagct tctgcagctt gtgtttcttg ttctgctttt tcttctgctt
    32401 ctttttgctc ttcagctgtt tggaacaagt cttggacttc ttctggtgta tttgcttctt
    32461 ttaattttcc tacgaaatct gcatcaatca gcaatcgaga tagtgctgct aatgcttgca
    32521 aatgtgtgtc gtttgcgcct tctggagctg cgatcatgaa aaacagataa gttggctgtc
    32581 cgtctaaggc ttcataatct actccttttt ggctttttgc gaataaaact gttgcttctt
    32641 taactgcact gtttttcgcg tgaggcatgg cgattccgtc accaagacct gtagaagttt
    32701 gtgcttcgcg ggctagaatt ccttctttat atgtttcgat atcggaaatc cggccggcat
    32761 caaacatttt ttggaccatt tcatcgattg cgcctttttt atcagtcgct tgcaaatcca
    32821 tgatcatcgc ttctttgacc attaaatcct tgatattcat gattatcctc cttgaaacta
    32881 atcaattttt gtgatttcaa cttctggcag caactcatca atcaattcgc gagctgccaa
    32941 gtcatcagaa aaagctgttg cgcttccgca agcaactccc catttaaagg cttcgattgg
    33001 atctttcgta cggctgtaag ccccaataaa gccagcaatc attgaatccc ctgcacctac
    33061 tgaatttttc aatgggcgaa ctaatacatt cgttttataa gttccttgtt ccgtgaagag
    33121 caaagcaccg tctccagcca ttgagatcag cacattctgt gccccttcag ctagtaattt
    33181 tttcccatac ggaatgattt cttctacaga ttggaaggtt gtgttgtaaa gctcagctaa
    33241 ttcgtgattg tttggtttga tcaacaacgg tttcttaaca agtgcatttt ttaggtcatc
    33301 acctgttgta tcaatcacga aatctgcacc tttttctcga atgatatcaa tcagttcttg
    33361 ataaaatcct tttcgcaggc tagctggcgc gcttcctgac aatacgacga tatctccttg
    33421 tcctactgtt cctaatgctt gcttcaactc atcgatttct tcatctgaaa tagatgggcc
    33481 taagccattg atctcagttt cagtgtctga ttttagtttg atattgattc ttgtatcttt
    33541 ttttacggtt gtgaacaaag actggatttg ttctttttca agccacttcg aaataaattc
    33601 gccagtgaac cctcctaaaa accctagtgc tgtagattga gaatcgatcc gtttcaaaat
    33661 acgggaaaca ttgatccctt ttcctccagg taacttgaag tcttccttca ttcggttcaa
    33721 atcgcctagt tttaacccat ctacgcgtac gatataatca attgatggat taagtgttac
    33781 tgtgtaaatc attgtattgc ctccttaatt gttgtctttt cacggaattc attaacgaat
    33841 tctaatggac aacgctcggt tatgatcgtg gctgcttcca aaggtgcaac agaggtgaag
    33901 gacaactggt tgaatttggt atgatcaatc aatacaaatg tctcttcgga atttttcatg
    33961 gctaaacgtt taacagctgc ctcttctgga tccggagtcg tgaatccttg ttcaagatgg
    34021 gcaccgttca ttcccataaa tgctttatta aaatgaaaat tttttagttg ttctatcgca
    34081 ttagatccta aaacagcatt agttgccagc tttatcgttc cacctaaaat aatggtagca
    34141 agctccaaat caactaaatt tgccgcatgt tgaacggaat ttgtcactac tgtgatctgt
    34201 tttccttgca gaaaaggaat catttctaac gtagtagagc cagcgtctaa ataaatgaca
    34261 tcttcgtcat taatggtttc agctgccgat ttggcgattt gctgtttttc gtgaacgttt
    34321 ttgagtgatt tttcactcat attctgctca aaattcaata attgctcttt cttagctccg
    34381 ccatgaactc tttctagaaa cccactattc tccagctctt gtaaatcacg tcttatcgtt
    34441 gattcagaag cattcaaaag gtttacaaga tcttgtgatt tcacgatttt ctgctgttca
    34501 agcaagtcta ggattttttg tctgcgttcc tctgtaagca ttttcacgat cacctcaata
    34561 caaataatag catatttttt ttctactagc aatataaatc agtcaaaaac gttcataaaa
    34621 attataaatc agtcaaaaaa aaaataaaaa accagtcaga aagactggtt ttttattttt
    34681 ttgtctatca tcaagccgtt ttatcgtttt ttattttcgc ttcttgtttt tttcatataa
    34741 acagaagcag gttttctttc cttatgaaga agataataat aaagtcccat cccgccaaac
    34801 atgaccaaaa aaccgatgac tgatcgaata ggaaaattag gaacaaaaaa gagacagacg
    34861 gataataatg atgccggaac cattattcga taatagagtt gtttatccat tttcttcacc
    34921 tcatattttt tgtaaagtaa tcaattgttc taattctcgg taatctaaaa attgcacacg
    34981 cagatgctgc tcacctttct tgacatcgaa ttggatactt cgccgctttt tctttattcg
    35041 tttgaaggta acatcagaga gtctatgttt gatttccttt aacggtaatt ctgacaggga
    35101 ttctgaagga acatagattc ttaaggataa aagatactct tgatattttt catctgttac
    35161 ctctacttct agatattccc taatataata acaataagct tcgattccat tatccaaagc
    35221 aacagtcgga tcgataactt tatagcacat catgcgtgat cgattctcgt caacccaagg
    35281 aaaatacgcc tgagcattta gtgttccgcg aacacgcctt tctttataat ctccttgttg
    35341 gatatcataa ctttttgtta caaaaaatga agcattcttt ttaggaacag gttgcatttg
    35401 tgcattctga tactggacag attggatcgt atggtcagtg taaatgtatt caacgaatgg
    35461 cggtttcaat aaaccgacac cattgtccca agcaagatga tcaaacccaa gctggtccct
    35521 aaaataggat tgaaacattt gattgtttgg cttttgccag ttaaaggggt ctcgataaag
    35581 ctggatacat atgacttcgt tttctaattc cttcactttt tctacttgtt ggatgtcgag
    35641 aaaaatcggt ttagggatga tttgatgata atagcgacta taatacattt cccaacacga
    35701 agtgaaacat aaaccttggt aaaaaagatc gtatcctgga aattggttgc aatcgactac
    35761 tacttccccg ttttgttgtt tcattttagg taaatgttcg atttcatcct ttttctcaaa
    35821 caatagccgt tgagagatct gttttgtatt gtgatagata aactcgctat aagctcgcat
    35881 ataggcgaac accccatgag caatcatttt tttctgaatg aagtctcgaa tcaaaaattg
    35941 cttttctttg aaaacagaga tagacaatac attttttatc aataattgat tatcttccaa
    36001 gcgaaaacta cattcgttct cgtcatttcc tacaactaac caactttcgt ctcttaccga
    36061 tgctagcatt attttttctt gaagctttcg aagatcatac ggatccgctt gttgtgtgaa
    36121 agagcggata aatgttggct ggatcaacag acgtgtcaaa atagcccaat cgcttatttc
    36181 ctgttttttt tcttcttcat ttaagtctgt gagctggata gtcatcaata atttatcttc
    36241 gccaatcatt ttctcacctc tttcaaatct ttttataatt taattataac caatgataaa
    36301 gaaaaactaa aactttatgt cttagaaacc aatttggacg attacatgat agactaagta
    36361 aaaagaaaca aagaggtgtt tacaatgggg ttgaaatttg aacgtacaga taatttagat
    36421 aaacttttcg aatcatttgc tgtcgatccg gaaaaaacag ttgcaccaaa agaagagatc
    36481 gatcgatttc taaaaccaca gccacaaaat gaaaagaaaa aagacaagaa acaaaagtaa
    36541 gttaagctca aaaaaagact taccagccaa acttttatga aagagtttga ctggtaagtc
    36601 ttttttattc tttttatgat tcaattgctt gttcgattgc tgcccacgct tcttcttttc
    36661 cctgttttgt aacggaagaa aagagaataa acgtgtcatt gcgatcaaaa tccaacgctt
    36721 ttttgatttc gctctcatgc ttgttccatt taccacgagg gatcttatct gccttagtgg
    36781 caacaacaat tacagggata tcataatact tcaaaaattg atacatctgg atatcttctt
    36841 gagaaggctt atgacgcaaa tctactaaag aaacgaccgc acgtagctgg tctctagtcg
    36901 ttaaatatgt ctcgatcatc tttccccatt ttgctcgctc agttttggat acttttgcat
    36961 acccatatcc cggaacatcg acaaaatgta atgcgttctc gatcaaataa aaattcaatg
    37021 tctgggtctt tcctggctta ccggaagtac gggctaaatt ctttcgatca atcaatgtgt
    37081 tgataaaaga agatttccct acatttgatc gtcctgccag agctatttct ggcaattgtg
    37141 tatctggata ttgttttggt gccactgcac ttatgataat ttcagcttga tggactttca
    37201 tggtctctct ccttaacaag gtgatgaata aagcaagcgg ctgtcgcaaa agccaaaatg
    37261 acttttacgg cagcctcttt tcatcaaccc gcttttttct ctgaatcttt atagattact
    37321 gttggctcac cagacaattc agctgcttct tttgtgataa tcactttttc gatgttttca
    37381 tttgaaggga tatcaaacat cacatccatc atgatttctt caatgatgga acgaagtcct
    37441 cgagcaccgg tattgcgttc gattgctttt ttcgcaatgg ctctcaacgc ttcaggttcg
    37501 aattcaagtt cggtattatc tagagaaaga agtttttgat attgtttgac tagcgcattt
    37561 ttcggctcag tcaagatccg taccagatca tctgttgtca atttctctaa agctgccata
    37621 acaggtaatc gtccgataaa ctcagggatc aatccgaatt ttagtaaatc ttcaggaatg
    37681 atatgctgca tgacactttc gtcttcagaa agtttttggt tttgtgtacc aaatccaatc
    37741 gtcttttcgc ccatacgatt cttcacaatc gtttcgatgc catcaaatgc accacctacg
    37801 atgaacaata tgttcgtcgt atcaatctga ataaattctt gatgtggatg tttacgtccg
    37861 ccctgaggtg gcacacttgc aacagttcct tctagaattt tcagcaaggc ttgttggaca
    37921 ccttcgccgg aaacgtcacg agtaatagat acattttcgc ttttacgcgc aattttatcg
    37981 atctcatcaa tatagatgat ccctttttct gcaagttcca cattgtaatc agcagattga
    38041 agcaatttaa gcaaaatgtt ttctacatct tcccccacat aaccagcttc tgtcaaactt
    38101 gtcgcatctg cgattgcaaa aggtacattc aatgtttttg ctaatgtttg tgctaaaaat
    38161 gttttaccag accctgtagg tccgatcaag cagatattgc ttttttgcag ttcaacatca
    38221 tctgtgtttt cttgtgcttg ctgattgaca cgtttatagt gattgtatac ggcaacagct
    38281 aacgagcgtt ttgcgcgatc ttgtccaatc acgtagttat tcaatacttc aagaatttct
    38341 agtggtttag ggacttcagt gaattcgcgt acagcttctt cataaaattc ttcatcaatg
    38401 atttctttac ataggtcgat acattcatta cagatgtaga cccctggacc agctacgatc
    38461 tttttcactt cttcttgggt tttcccgcaa aaagaacagc gtacggtctc attgctactt
    38521 ggattgtcat acatggtctt caccccttaa cttgcaaaaa gtatgcaaga aactatcaaa
    38581 gactttacag tcttttacta tcataacata gtttagtaaa aagaaaaagt gtttaacaaa
    38641 ggatcaactg acctatacct ttgcattttt tactcttaac aaaaaataag gaactgtgac
    38701 aaaatcgtgt cacagtccct tatttcgaat aaacaatttt ctagaagtag cttcttcaga
    38761 aatgatttat tacttgattt ctattctttc gcttctgtcg ctgtacctgt gatcaattcg
    38821 attgcttttt tcattgtcac atcatgttcc agcatatctt ctgtcaacac gcgtttgatt
    38881 tgttcgattg gcatgttgta tgtttctgat aattcttgga tttctgcatt gatttcttct
    38941 tctgttgctt gaagattttc agcagctgca attgcttcga tcacaagatt tgttcttgtg
    39001 cgcatttctg cttcaccttc gaattgttta tgcaagtctt cttctgtaga accagttaat
    39061 tgatagtaca tttcaggaga gataccttga cgttgcatgt tgttcaagaa ttcgtccatt
    39121 gaacgatgta cttcatcatg aaccatcacg tgtggcaatt caacgatttc tgcgttttca
    39181 acagccatac ggatcgctgc ttcatctttt gcatcgtccg ctgctttttc ttttgtttct
    39241 actaattctt tgcgatattt ttctttcaat tcgtctaatg ttgatacttc gtcatcaaca
    39301 tcttttgcga attcgtcatc taattcaggc acttcttttg cttttacttc atggattgtc
    39361 actttgaaga ctgcttcttt tccagcaaga tcttctgctt gataatcttc tgggaaagtt
    39421 acatttacat ctagactttc acctgattta tgaccaacta attgatcttc aaaacctgga
    39481 atgaatgagc ctgaacctaa ttcaagtgag taattttcgc cttttccgcc atcaaacgct
    39541 tcgccatcta caaagccttc gaagtcgatt actactgtat cgccattttc agctgcagca
    39601 tcttctttga taactaattc agcttgtgct tcttgttcac gtttgatacg tgcatccacg
    39661 tcagcatctg ttacttcacg gtcttgtttt tcaacaacta gatctttata ttgtcctaat
    39721 tttacttctg gtttcactgt tacttcagca gtgattaccc agtcttgacc tttttccatg
    39781 ctttcaacat cgatttttgg ttgagaaaca ggatcgatgc cagcttcttt gacagcagct
    39841 tcataagctt ctggtaatac agcattcaat gcatcttcat ataaagcttc ttcaccgtac
    39901 atacggttga atacttgacg agaaactttc cctttacgga atccaggtac gttcaagctt
    39961 tttttcactt tattaaacgc agttgttaat cctttttgga tttcgtcttg cgcaatcgta
    40021 aattttaaaa caccatcatt cgtgcctttt ttttcaaatg ttgcagtcat ttaattccct
    40081 ccgagtttag aaattcattt tatacatcaa aaacgtttca tgcatacctt taaatagtac
    40141 actaaccctt tcaaattgta aacatattga atggatagaa tccttgtttt tcataacatt
    40201 tttatctttt tcactgataa aatctaatgg aagaagatca tttgctgcaa aattttttct
    40261 ctttttttta aaaaatcttg tttttcatct tctgtacctt catacatcgt ttttcctgta
    40321 cacgcctcta cagtcagttg aaaccagcgc ttcgggtctt cgatgtatgt atcatagaca
    40381 ggataaacaa ttgcagattg caacgtgaat tcttctttta acacggaact aagcagcgca
    40441 tcttcattcc cgtaacgatc tgaaaataca cataattgct ggtaacttgc ctgctgctgc
    40501 ggctttggca gatcttttgg tatgactttg actaactttt cttcaatcgt caaatagaaa
    40561 acaggatggc attcgttcaa ttgcctaagt gtctccagta attttgccct tgctaaaata
    40621 tggacctctg gcgatacaat aagtttactc gctgtttgga taaactcatg ataaggaagc
    40681 tgttcgatct catcaataga cgcaagctga cgtgcaggtt ccaacgaagg aagttcctcc
    40741 gatttattgt ggagtgcctt gatcttctgc ctctccattt gtccggataa ttcttctgca
    40801 aattggattt tttcaatgac ccgctgctcg aaagcttcat aaaaatctgt acttgctatc
    40861 aattttctag catataaaaa atcttgtgta ctgattaaca agtcaaaata aaattcagca
    40921 aactcctcat tttgaagata ctgtttttca tgcaagagtg cttgcttcaa tgcttctttt
    40981 ttctcgtcca actcgtaaag acaaaaaaca agcaggctat tagcctcaaa gctttctttc
    41041 aactcgtaag ctcgttgaaa taattctttt gcctgctgat attgctggtt tgctagtgct
    41101 tgttgtgcca ctcgcattaa attctcatat ttttctggaa aatcaatcgg cttcacgcag
    41161 tgtcctcctc ttgctcatat tcagctcatg ttcaatagta gcaaaacaaa aaaagcatga
    41221 caattaaaaa taactgaagc accctctaaa agctagattt tagatctgac ttttagaagg
    41281 tgctttcaac tgttactgct ttttattgct catcagctgt tgacttcctg gttgaacgct
    41341 ttgatcagat tgatcgtaac aacacttgta ttagtgaaaa aatcgcccag atgttgcttt
    41401 ttctgattaa agactgctgg aagataccct aacatcagca aaggaaattt tagaatgaac
    41461 cggcaagcac cttctctaac taaaacggtt gaccagctaa gttcttcttc atcaaagctg
    41521 atgactcgaa taccaaagat cattttccca atcgtttggc catgattgaa tttcgtcaat
    41581 aaaataaaat acgctagata aataagtaag gctgtgaatc cgtagagact cagataagaa
    41641 tctgaaaaat taagccaacc tagatttttg gcaataccaa gtgttaaacg cgtaatagct
    41701 ccgatacaca aaagatcgac caagaaggca aacattcgga taaaaaaacc tgcatagaaa
    41761 tacttaggaa aatcatttgc ttttggcggt tcatttcttg tgtccgtata ctttttccat
    41821 ttacgttgtt tctttttgat ttcttcgggc gtcaaaggct ctctttcaaa agagctcagt
    41881 accttttggg aaaagttgga gtcaggtcct acagcaggct gctgttgctt atctttttct
    41941 tgttccttat tttcatttga aagctctgac atgcttactc acctccgtaa taatacattg
    42001 cttttggtga ttccgctgtc cctagattct caagaacgga taagatctga ctagtctcag
    42061 aaggcttcaa tccctggaat tctgctagtt tacttcctaa ccatgtacta gcaaacccag
    42121 atggcgtgga agaatattct actagttccg catcttcaag ctgttgttcc tttcgtatag
    42181 cttttaaagc atcttcagga aatcccaatt catcgactaa acctacttct tttgcttgga
    42241 caccatcata aatccttcca tcagcaattt ttttgacttc ttcttcagat ttattccgtc
    42301 cctcgctgac gattgctaca aaacgactat atgcgctgtc gatatatgct tgaagcaccg
    42361 cttcttcttc ttttgtctct ggtcgcgttc ctgaacccat atctttcaac gctccgcttt
    42421 taactgttgt atcttccaca cccagttttt ctagaagtcc tgaataattc aatcctgaca
    42481 tgatcacacc aattgagccg gtcactgttt cttctgttgc aaagatttta tctgcttgag
    42541 cagaaatata gtagccaccg ctagctgcca tgtttttcat gctgacatac attgggatat
    42601 catgttcttt acgaatcgta tccaatttct tagcgatttc cgcactttcg tagacaccgc
    42661 caccaggaga atttacttct aaaaagatac ctttgactgt tggatcttct tcaatcgcct
    42721 tcagttgttc cataaaattt ttatgattgt aaccttcaga agcaaataaa cctgaagacc
    42781 ctcccgcagc aatcgttcca tctactgata attttacgat tttttgcgta ctgtctccct
    42841 cttccaacac atttggagct agttcttcat ccccatataa ccaactgttc aatccctcaa
    42901 tttgatcgtt ttcctgaggc ttagtcaaac tagctgaaac taacgaaacc acaaataatc
    42961 ctgcagcaat cagaactgct gcccaacgtc ttttattcat tctttctcct ttctaataca
    43021 agttttttat gacgatcaca gtttctgact gctcagaagt gtcgaaccgc tctgcttttt
    43081 cttcccgcgc atacccggaa aacgactctt ttgttaattc atcttctacc gtaaactcac
    43141 gagcttgagc aacttccaag gcatcaggtg cgatttcttg gatgctatcc cagccagtat
    43201 ctaaaaatat tttcgtatca tctgatacac cagctgatgc ttcgaatttg gacaattgtt
    43261 tttttaacat tcccagtgtc atgatctcaa tttttgaatc catttttact cctcctctat
    43321 ccgattactt tctattgtac cgaactcctg aaaaagatga tagataaaaa cccattattt
    43381 tttgacataa aaaggacatt tgacaaagaa tttgatatat accaatttat tttttattct
    43441 ttttttgttg acaagaacat tcgcaaaagg ttatgatttt aaatgtttcc agataaaaaa
    43501 caaaaaggag gatagttata ccttttagga tagcgccagg tctgtgtaaa cagatgacga
    43561 ggagagggtt tatcgaaaga ttcggcggat ggccctaggg actgcactct acagaaaatc
    43621 cacaaaaaat gtctgcaaag ataaaacaaa aggattttcc tgcagctgga agcattcata
    43681 cgaattcatt atagaaagta ggatgttttt tatgtgcgga attgttggaa tgattggatt
    43741 aaaaaatgta acaccaggat tgattgacgg tctagaaaaa ttagaatacc gtggttatga
    43801 ttcagctggt atctttgtct cagatggcac aactgattat ctggtcaaag cacaaggcag
    43861 aattcaaaat ttgaaaaaca aaataaatat tgatactaca ggaaatatag gtattggtca
    43921 tacacgatgg gcaactcacg ggcaaccttc tgaagaaaac gcccatccac atacttccca
    43981 aagtggacgc ttcgtgctcg ttcacaacgg cgtaatcgaa aactttgaag aacttaaaat
    44041 tgcttattta gcaaatgatc actttattgg tgagaccgat actgagatta tcgctcatct
    44101 aattgaaact ttcgcgaagg atactacaac gcaagaggcg tttttaaaag ctttgcgggt
    44161 catcaaaggt tcttacgctt tcgctttgat tgatcggaca gcacctgatg ttatttacgt
    44221 cgctaaaaat aaaagtccac tattaatcgg actcggcgat ggattcaatg tgattgccag
    44281 cgatgcaatg gctatgcttg ctcatacgaa ggaattcgtt gagattgaag atgaagagat
    44341 ggttaccgtc acatcagaaa aagttattat tcaaaatttt gcaggtgaca tcgtagaacg
    44401 ttcttcattc gaagcacaag tagatgcttc agatatcgag aaaggtacct atccttttta
    44461 tatgctgaaa gaaatcgacg aacagcctat tatcatgcgt cgtatcgctc aaaaatacgt
    44521 aacagaagac aatcgtgtct ctcttgatca aaagctggtc gatacattgt ctgatagcga
    44581 tcgaatctat atcgttgctt gcggaactag ttaccatgcg gggttagcag gtaaacagac
    44641 tttggaaaaa ttgacacaga ttcctgttga ggttcattta gccagtgaat ttggctataa
    44701 cacacctctt ctttcaaaaa aaccattctt tattttcttg agtcaaagtg gcgaaacagc
    44761 agacagccgc caagtgctgg taaaaatcaa ccgtctcggc tacccttcac ttacgattac
    44821 aaatgttgct ggttctactc tttcgcggga agcatccttt acactgctat tgcatgctgg
    44881 acccgaaatc gcagtagcat caacgaaagc ttatactgca caaatagctg tactaacttt
    44941 attagccaaa gctatcggtg ataaaaaaga atttgaaact tctcttgtat tcgatatcac
    45001 tcatgaattg agtttagttg ctaatgcgat ggaatcggtc atcgcccaaa aagattattt
    45061 agaagagttg gcagaagtat atctcagcga tacaagaaat gccttttaca ttggacgtgg
    45121 tgctgactac aacgtttctc ttgaagctgc tttgaaattg aaagaaatca gttatatcca
    45181 ggcagaaggt tttgccgctg gcgaattgaa acatggaacg attgctttga tcgaagaagg
    45241 aacaccagtg atcggaatca tcagtgaaga agtgactggt gcgcatactc gaggaaacct
    45301 gaaagaagtc gaaagccgtg gcgctaaaac actagttatt gtctcagagg gattagaaaa
    45361 ggaaagtgac cagctggtgc taccaactgt ccacccttat ctaactactc taaccacagt
    45421 cgtaccgact caattacttg catattacgc aacgctatta cggggattcg acgttgacaa
    45481 accacgtaat ttagcgaaat cagtgacagt agaataataa aagaacaagg accagtgtca
    45541 gacatctgac actggtcctt gttcttttat tttttatgaa aatatctctt aatccagtcc
    45601 gatttcttct ttgactactg ccgcgatttt atcgacatag taattaactt tctcatcagt
    45661 aggcgcttct gccatcactc gaagtaatgg ttctgtaccg gaaggacgaa ctaggattct
    45721 tccttctgat cccatttcag cttctgcctc ttcaatcact tttttgattg ccggaacatc
    45781 catcgcaccg tttttatcac ttacgcggat attcactaat ttttgcggat agattgttac
    45841 ctctgctgca agctctgata atttcttacc tgtctgtttc atgatattca gtaactgaat
    45901 tcctgaaagc atcccatctc cagttgtgtt aaaatctaag aaaatcatgt gcccagactg
    45961 ctcgccgcca aagttataat cgtttttgcg catttcttct acaacgtaac gatcaccaac
    46021 ttgtgtaacg acatctttca aaccgattcc ttccaccgct ttgtggaatc ctaagttact
    46081 catgacagtc gtaacgatcg tatctttttt caatcgattt ttttctgcca agtatttagc
    46141 acagataaac atgattttat caccgtcgac aatgtttcca agttcatcca ccgcaataat
    46201 acgatcgcca tcgccatcga aagctagtcc agcatctgct cctttttcaa cgaccatctc
    46261 agctaaacgt tctggatggg tagagcctac gccgtcattg atatttaacc cgtctggatt
    46321 tgtgcccatt gtataaaaat ctgtttcaag atcggcaaac aaacgattaa cacttgttga
    46381 agttgctccg tttgctgcat cgacacaaat ggtcaaaccg gaaaggtcac cgttgatcgt
    46441 ttgtacaagg aattgagaat atttcaataa gccttctgga aattcttcta cagttcccaa
    46501 tccttcagct gatggacgag gcaattcatc cacttcagca tctaataatg cttcaatttc
    46561 tagctcttgg tcatctacta gtttaaaacc atctgcaccg aaaaatttga ttccattatc
    46621 ttgtgctgga ttatgagaag cagaaatcat gacccctgcg cttgcttttt gcaaacgtgt
    46681 cagataagca acacctggtg tagagatcac acctagctgg aacacttcga tacccaccga
    46741 taaaagtcct gaaatcagcg cttcttctaa catttgacct gagattcttg tatcccgccc
    46801 tactaacacg cgtgggcgtt ttccttcttc ttcatgctgg cttaggacat aacctccgca
    46861 ccgtcccagc ttaaatgcaa gttctggcgt aagttccttg ttggcttcac cgcgtacgcc
    46921 gtctgttcca aaatatttac ccatagttgt aaaattactc ctttaactta attttctgag
    46981 gaatttgttc tgctcatttg tgttgattcg cttgtttctg atgtttcttc tgtacttgtg
    47041 gtcgagctgc ttgtcccaga ttccgaactg gaagaaatgg ttgttgtcga tggttccgtt
    47101 cctgaatcag cagatgaaat acttctactc gtacttgtat tttcttgtgt ccgactgctc
    47161 gtcgaagcag agctactgct tgacgtttgc aacacgggtg taatctccac actaactgat
    47221 tcgggcttga tacttaaccc ctcttctact ggtatttcaa ttttacgagt tgttcgatga
    47281 ttgatacctg agacattgat cggtacagca atactgtcac cgatcgatgc aagcaaatcg
    47341 ctagagccgc ttacttctgc ttccagtttt gaaaaactga aatcatagct ttctaccccg
    47401 gcagctggtg ttccttgctg tatcccataa agtcgtaccg acttgcttgg cttgatcgaa
    47461 tcagctctca ctgttacttg cactggatca gagacgatac ttaatacttc ccctgctgca
    47521 ttcagcgcct gtattgggac ctcactatta atcccatcat ctgtgaccat cgaagcgtcc
    47581 acatcagcta ctactcgttt gatttctgcc aatgttttgt cacctgtagt gatagagact
    47641 tttttaggac taacggaagt atcatcaagt tcatatccag atggtgtgaa cgttgaagga
    47701 atcaacgttt ccacatcgaa atcttttatt acctttttct caaccgttac tgtaatagtc
    47761 gatggttcga tctttgctgt gacagcactg ctcaaatttt gaatttttag cggaacttca
    47821 tgtgttcctt cgcccagctt agtgatatct gctgtgacgc ggaacatccg tgtatcttcg
    47881 tttgcctctg cattcaactg aactcggttt gcactggaaa gtttgacagt cactgcattt
    47941 tcatatccat ggatatagta gcgctttgaa tcataagaaa tcttgatcgg cacccccgta
    48001 actgtttgat tatacgcttc atttccagaa agcgtatttg gaacggactg gccatttgca
    48061 ttgaagtaca gcgcaatact gaacagcaat gcaataaaac tgtaaagaat gcttgtccgt
    48121 ttctcttttg agatcatttg cgatcccctc ccttagtcat accgtctagg aaagattgaa
    48181 tcacgttttt cttgtttttt gttttctctt cagcaattag ttctctacgc aagattgcca
    48241 aatattcttg ctgggataag ccagccatca gttcattatc taacgtgatg ctgacgtccc
    48301 cggtttcttc agagacaacg atggtaatcg cgtcactgac ttcgcttata ccgacagctg
    48361 cccggtgtcg agtcccgaat tctttaggga tcaacatact ttctgataga ggaagatagg
    48421 cgcttgctac tgcgatcttt ccttctttta caataactgc accgtcatgc agcggtgtat
    48481 taggaataaa gatattgatc agcagctccc cagtgatatc tgcatctaaa gcaatccctg
    48541 tctcaatata ctcatctaat ccagtatgac gttcgatagt gatcaacgca ccgatttttc
    48601 gcttggacat atattggatg gctttgtcaa aagacaagat catcttctca tcttcctgct
    48661 gttcgctttt tgattgtttg aaaagcgaac tgcgtccaag atgttccagt cctctccgga
    48721 tttccggctg aaaaatcacg atagcagcta tcaccccgta cgtaatgact tggttcatca
    48781 gccaagacaa ggtgtgcaga ccaatgattt ccgctacgat tcggataaca ataaaaatag
    48841 ctacaccttt cagaagctga atcgcttttg tgcctcgcag cagttggatc agtttgtata
    48901 caagatacca gacaaccaga atatcaagta tgttcaccaa aaaattcaga gagaacaagt
    48961 caccggaaaa catttgctgc caatagtcca aattaaaaag ttccccgatt tgaaatgaca
    49021 taaaaagcct ctcttctctt ttgagatttc ctctctagta tatcatatgt tttaatttta
    49081 gaggctgata tgctcgtatt tttcttgaga aaaacgcata attttcaaga aaattgcaag
    49141 tatgcgtttt atttttctct gcaataaata tttttaaact tcagagaatt attcattata
    49201 ttttttctgt tatttttcct ttttttcttt tgtagcttgt tccatttttt tacgtcgttc
    49261 tgctctggca gctcttctag cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    49321 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    49381 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cgtggacagt
    49441 cttttcaaat aaagcttttg caagatttgg atttgcgtta tctagcgagg aaaagcgaac
    49501 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    49561 tgggttcttg tttttttcga ccattgtagg gttatatcga tataatgacc aataaccaga
    49621 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccgccaagca agccatgtgt
    49681 gatacaaggc gtataggcaa taatcagaga aggaccagga tacttttctg cttcttcaaa
    49741 tgccttgatc gtttgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    49801 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    49861 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    49921 ttcgttatcc aaaacaagga tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    49981 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    50041 gaatagatcc tttctcttgt atatatcctc cagttcaggg aaaattgatc gttcgctttc
    50101 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcactctcaa acatgtgtac
    50161 taaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    50221 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    50281 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    50341 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    50401 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    50461 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    50521 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    50581 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    50641 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    50701 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    50761 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    50821 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    50881 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    50941 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    51001 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    51061 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    51121 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    51181 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    51241 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    51301 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    51361 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    51421 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    51481 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    51541 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    51601 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    51661 ttctgtcaaa ttcagacttt cctttggttc caaagattga taagtcacat catccacgat
    51721 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    51781 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    51841 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    51901 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    51961 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    52021 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    52081 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    52141 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    52201 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    52261 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    52321 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    52381 acggaatcca tcaaagaaat tcaaaaaagg gacttctcct tcaatagaag caagatgggc
    52441 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    52501 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    52561 tgcccgacta gcaacgtgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    52621 cggtatcatc aaaagcagcc cttgtgaagc ggtatatgta gttgtcagtg caccagtttt
    52681 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    52741 tggttgaccg aatacgtttt cttttcctgc tgctgaccat tgatctacta attcagccat
    52801 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    52861 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    52921 atattctctc ttattatctg ttccattata acgtaaaaca tgccattttt ttcttttatt
    52981 tataaaaata ttttacatgt atctatttga attggtcatc taatttttag acaaacagtc
    53041 taaatcgttg cctttttttg tcagacatgt ataaactata agcgaaagag gtgttttaac
    53101 atggatatat cagtgattga tgcaacgaaa acaaatacac aaaaaggaat cctttatggt
    53161 tcatcgaatg ctcctaaaaa aatggtcgaa ttcatcaact tagcttgtcc gtattgccga
    53221 caatggtttg aagaatctta tgagctatta gaagaagcag tccaaagcgg gcaattgcag
    53281 cgagtgatca aattgttcga taaagaaaaa gaaagcttac agcgagggaa cgtgatgcac
    53341 cggtatctca caatcagcga tggccaaaaa gcgatcaaag agatcaaaca aatattcgat
    53401 actcaagacg agtggaaaca tctttctctc caagaagtag cggattttgc agtagataaa
    53461 ctaaagctga cagaacaaaa agacgaacag ctgagccaag ctgtcatcaa cgaagctgag
    53521 caagcacata ttcgatttgt tccaactgtc atcttgggta aagaaatctt tgatgaatct
    53581 atttcaatag aggaattaaa agaattgatc caaacaaaat aaaaaaataa
//
LOCUS       DACCKU010000008        49745 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.008, whole genome shotgun sequence.
ACCESSION   DACCKU010000008 DACCKU010000000
VERSION     DACCKU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 49745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.008"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(154..1740)
                     /locus_tag="IVN13_000434"
     CDS             complement(154..1740)
                     /locus_tag="IVN13_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP7010953.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            1991..2215
                     /locus_tag="IVN13_000435"
     CDS             1991..2215
                     /locus_tag="IVN13_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP7010954.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            2357..4108
                     /locus_tag="IVN13_000436"
     CDS             2357..4108
                     /locus_tag="IVN13_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7010955.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFVIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            4108..5892
                     /locus_tag="IVN13_000437"
     CDS             4108..5892
                     /locus_tag="IVN13_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7010956.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQTITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            6349..7200
                     /locus_tag="IVN13_000438"
     CDS             6349..7200
                     /locus_tag="IVN13_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7010957.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSEQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGEKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            7296..9182
                     /locus_tag="IVN13_000439"
     CDS             7296..9182
                     /locus_tag="IVN13_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7010958.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIRAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILMMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGNLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEADETDDRQKTSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFKSL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(9223..10125)
                     /locus_tag="IVN13_000440"
     CDS             complement(9223..10125)
                     /locus_tag="IVN13_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7010959.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            10355..11758
                     /locus_tag="IVN13_000441"
     CDS             10355..11758
                     /locus_tag="IVN13_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7010960.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            11758..13191
                     /gene="ascB"
                     /locus_tag="IVN13_000442"
     CDS             11758..13191
                     /gene="ascB"
                     /locus_tag="IVN13_000442"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7010961.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            13336..14262
                     /gene="trxB"
                     /locus_tag="IVN13_000443"
     CDS             13336..14262
                     /gene="trxB"
                     /locus_tag="IVN13_000443"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP7010962.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            14476..16203
                     /locus_tag="IVN13_000444"
     CDS             14476..16203
                     /locus_tag="IVN13_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP7010963.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIQKMTEE"
     gene            16276..16569
                     /locus_tag="IVN13_000445"
     CDS             16276..16569
                     /locus_tag="IVN13_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7010964.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(16664..17524)
                     /locus_tag="IVN13_000446"
     CDS             complement(16664..17524)
                     /locus_tag="IVN13_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP7010965.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            17616..18044
                     /locus_tag="IVN13_000447"
     CDS             17616..18044
                     /locus_tag="IVN13_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7010966.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            18206..18382
                     /locus_tag="IVN13_000448"
     CDS             18206..18382
                     /locus_tag="IVN13_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP7010967.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            18409..18855
                     /locus_tag="IVN13_000449"
     CDS             18409..18855
                     /locus_tag="IVN13_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP7010968.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(19011..20390)
                     /gene="brnQ"
                     /locus_tag="IVN13_000450"
     CDS             complement(19011..20390)
                     /gene="brnQ"
                     /locus_tag="IVN13_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7010969.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGTNWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            21096..22067
                     /locus_tag="IVN13_000451"
     CDS             21096..22067
                     /locus_tag="IVN13_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP7010970.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            22091..24283
                     /locus_tag="IVN13_000452"
     CDS             22091..24283
                     /locus_tag="IVN13_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP7010971.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLFAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            24312..24788
                     /gene="ybeY"
                     /locus_tag="IVN13_000453"
     CDS             24312..24788
                     /gene="ybeY"
                     /locus_tag="IVN13_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP7010972.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            24766..25167
                     /locus_tag="IVN13_000454"
     CDS             24766..25167
                     /locus_tag="IVN13_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP7010973.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIVGLILFVPKIYHLLFN"
     gene            25182..26081
                     /locus_tag="IVN13_000455"
     CDS             25182..26081
                     /locus_tag="IVN13_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP7010974.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            26224..27036
                     /gene="recO"
                     /locus_tag="IVN13_000456"
     CDS             26224..27036
                     /gene="recO"
                     /locus_tag="IVN13_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP7010975.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKDMSEKDLPKKEE"
     gene            27422..28339
                     /gene="glyQ"
                     /locus_tag="IVN13_000457"
     CDS             27422..28339
                     /gene="glyQ"
                     /locus_tag="IVN13_000457"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP7010976.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEFESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            28341..30416
                     /locus_tag="IVN13_000458"
     CDS             28341..30416
                     /locus_tag="IVN13_000458"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP7010977.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEIIPFEILDVKTDRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     KEMIITQANELAEKNHWIIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGDLPETTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISE
                     VISKDTDRFGIGLSAGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKTSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            30806..31585
                     /locus_tag="IVN13_000459"
     CDS             30806..31585
                     /locus_tag="IVN13_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010978.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSQIEQIRNEKISVAYATKAQTVAQQMGYSNINY"
     gene            31631..32575
                     /locus_tag="IVN13_000460"
     CDS             31631..32575
                     /locus_tag="IVN13_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7010979.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            32591..33370
                     /locus_tag="IVN13_000461"
     CDS             32591..33370
                     /locus_tag="IVN13_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP7010980.1"
                     /translation="MEETISLKEIFGTLRKHLATIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISINQSENSQMFSIQAISTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGSAPARPKATNDKTAPSRRSRSRV"
     gene            33382..34089
                     /locus_tag="IVN13_000462"
     CDS             33382..34089
                     /locus_tag="IVN13_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP7010981.1"
                     /translation="MARTQKQKAAQTHAVSLITLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVVYKTFQLNNAS
                     GLSTALSSSGNVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDAGYYYTEIKE"
     gene            34110..34874
                     /locus_tag="IVN13_000463"
     CDS             34110..34874
                     /locus_tag="IVN13_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP7010982.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEWLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQITAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            34937..35626
                     /locus_tag="IVN13_000464"
     CDS             34937..35626
                     /locus_tag="IVN13_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7010983.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFSHTRVGKNGKTFKMHKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTQIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTKYDLQRLCVKPGCSGLWQVSERNDVSFHKMVELDLEYIGHRSLMYDLRLIIKT
                     FYIIIKPNGAY"
     gene            35962..36279
                     /locus_tag="IVN13_000465"
     CDS             35962..36279
                     /locus_tag="IVN13_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010984.1"
                     /translation="MYTNSIENVCKSKEIILFSVPSVFVRNTAQKVKPYISDDQIIVD
                     VAKGIESDTLMTLTEIIEDELSEHRIHLVVLSGPTHAEEVAMQLPTTIVSASKNQDIA
                     KRV"
     gene            36301..36411
                     /locus_tag="IVN13_000466"
     CDS             36301..36411
                     /locus_tag="IVN13_000466"
                     /inference="COORDINATES: protein motif:HMM:NF019122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010985.1"
                     /translation="MRVYTNNNVRGIEICGALKNIIALAAGISKGMGYGE"
     gene            36401..36667
                     /locus_tag="IVN13_000467"
     CDS             36401..36667
                     /locus_tag="IVN13_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HAP7010986.1"
                     /translation="MVNNAKAALITRGLAELERLVGRIGCSPKTFSGQLIGQGVPTEL
                     AIKQVGMVVEGINALPAAVKLSKQFQVELWQLMQLSMAERSQKN"
     gene            36769..37164
                     /gene="tagD"
                     /locus_tag="IVN13_000468"
     CDS             36769..37164
                     /gene="tagD"
                     /locus_tag="IVN13_000468"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP7010987.1"
                     /translation="MKRVITYGTFDLLHYGHINILRRAKELGDYLVVVLSSDEFNWNE
                     KQKKCYFTYEQRKQLLESIRYVDLVIPETCWEQKLSDVHEYHIDTFVMGDDWEGKFDF
                     LREEGVEVVYLPRTPEISTTQIKKDLNKV"
     gene            37286..37735
                     /locus_tag="IVN13_000469"
     CDS             37286..37735
                     /locus_tag="IVN13_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HAP7010988.1"
                     /translation="MKVCLVGSSGGHLAHLYMLKPFWENTDRIWVTFDKVDAKAILKD
                     ERMIPCYFPTNRSLKALIINSIIAWRTLRKEKPDLIISSGAAVAVPFFYLGKLFGAKT
                     IYIEVFDRIDKPTVTGKLVYPVTDRFIVEWEEMKKVYPKAINLGSLF"
     gene            37770..38279
                     /locus_tag="IVN13_000470"
     CDS             37770..38279
                     /locus_tag="IVN13_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HAP7010989.1"
                     /translation="MVFVTVGTHEQQFNRLLQAVDSWAENREETVVVQTGYSTYNLVH
                     CISEKFFPYNEMMDYVQKARIVICHGGPASFIMPLQYGKVPVVVPRQVKYEEHVDNHQ
                     LDFCKEVEHRMGNIIVVEEISDLTDILDKYSGIINDKDKQLSSNNELFCKNFSIVVDE
                     LLKNRGSQI"
     gene            38281..39258
                     /locus_tag="IVN13_000471"
     CDS             38281..39258
                     /locus_tag="IVN13_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7010990.1"
                     /translation="MFKYDLSLIIPVYNAERYIEDTISAVVNQTIFPNVEVIIVNDGS
                     SDDTESICEKYVKDYENIKLITQNNMGVSEARNSGLRVATGNYVTFLDADDFVEADLY
                     EKELQLIKDNSADIGVVDFKKVHDDGTIVKYRKNYVRTWNGDNSSIIKDFLSGIIGNQ
                     VVDKIFSKAIINGLSFSGKYKIGEDMLFMYQALERAEKVVMDTSIADYHYIVRSDSAM
                     TGKFDEKYFDPVIISEIILDSCAYEKELKHYAQAHLIHEICKSCEYIYRHKAEKMCPD
                     KLKYLRNKLAEYSVLDAKKYLIKKQFLGFLLMRFVPKIYMLAHRMMHIG"
     gene            39270..40265
                     /locus_tag="IVN13_000472"
     CDS             39270..40265
                     /locus_tag="IVN13_000472"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7010991.1"
                     /translation="MQDKNLSIIVPIYNVADYLERCMNSIIAIKNFKYITVILVDDGS
                     TDSSGLIADEYAKRFDNVCVYHKVNGGLSDARNYGLVRADTEYVFFIDSDDMLVPETF
                     DLVISELEQSSCQVLLFDAKVVDEKDGYLETVDGNYYVHGGLRSNCCYTGKDVIELQL
                     TDHNDFVTTVWLGLYNRDFLIQNNLWFEKGLLHEDELWTIKTLLNAREIRYLMESAYL
                     YRQRAGSIMSSNNRNWNKNIQAVIFIYQSLYAYVDWKVSDIEFARMLKGNLTRRYVHA
                     LSKFEVYKYREIYKKIQFRVLYMNCVRPIDKIRVLILKFTPNMFQWITKLIGKSR"
     gene            40482..41699
                     /locus_tag="IVN13_000473"
     CDS             40482..41699
                     /locus_tag="IVN13_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAP7010992.1"
                     /translation="MEILYALFTAIFCTGRFWGIDVEWYISVGVRILIIGLFLVFHKF
                     RFKRNYIIEKNLKLATVPIIAIVVYSIAVWLMSGSVPDEDVVRNLFTANTYLVISYLF
                     ASVLYYEFGKNSIVFLMKCGVVSYLLGSILPLIIKMPRAAIPYLFTSHTNDTSLLWLT
                     EVNDLTFAFGIILLYFLFSYHQNHKKKNIIICALMIFWGLKRIEIAAILLCYLFFKVI
                     TEKIVFSQGAKIFLVSVLSVSYLYVMFIHNGQLAMLAEMYNINFMGRLLTYNYVTETY
                     SSFSPGFLGIGFGRIDEILDDLVAENFRINYVPVISLHSDVLRMYIGLGFIAFGIWIF
                     YQCYYRTMKLKKYFGSKTAQIYICLTLYIFILYLTDNTYSYPITGATYFLAILCVSDE
                     NHFNAYLNKERAK"
     gene            41696..42700
                     /locus_tag="IVN13_000474"
     CDS             41696..42700
                     /locus_tag="IVN13_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7010993.1"
                     /translation="MTEKLSSVCLISVYFGKFPDWINVWFLSLKHNPTIDFILVTDQN
                     VDTSGYDNLKVINTDLDSFKMLLQSKLGMKNSSYKLCDYKLVWGLALENEISKYDYWG
                     HSDMDVVYGDIRSFLIRYKLCDYDKFGNRGHLTLYRNTKEVAERYRLTGSWFGSYKEI
                     FKSNRIWAFDETPGINAIYNAHNFSHMNSFIYADIDVNYKDLRLVDRNDPQYVNYQNQ
                     VFYWKKGKLLRAYSKDGTMVVDEFMYIHLQKKRMQKPDFNLEEFDGFYILNGYFEPLG
                     SCITLDDVLKRNIRNDIDLPQKMFGGGYKKDLRFYWLGRRYLNMKYDLKQVIKKLLKR
                     "
     gene            43185..44114
                     /locus_tag="IVN13_000475"
     CDS             43185..44114
                     /locus_tag="IVN13_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAP7010994.1"
                     /translation="MKIKKIKEYIKKGTIFSLLRYKVSVFLLKKCTHNKNLAVLSRQC
                     IVGEKLKKEFSYVLENKYKDPFEGKSNKVWFCWFQGYDAAPELIKACYHAAKQAMPNK
                     EIIFLTGDNINSYVHLPDYITEKYHSGKIGFAHYSDLIRISLLCEYGGLWIDSTVLTT
                     SPEMAEYITSLPFFVFKQLELTRLDYQPTVASNWLISSYSNNKILLLTRDFLYEYWKK
                     YNYAIDYFIFHIFFRFATEKYREDWENVPTFNNISPHMLQMELNSEYNEERWKQLLLI
                     SGFHKLNHHIAYNRDNTFFEYVLNRYKSFEEES"
     gene            44111..45565
                     /locus_tag="IVN13_000476"
     CDS             44111..45565
                     /locus_tag="IVN13_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HAP7010995.1"
                     /translation="MKNNQDNLKYQVMGGMFWKLSERILAQGVSFLVSVVLARMLTPD
                     EYGIVAIVLVLISLANVFVTSGFSVALVQNKNSKDIDFSTNFWCSIAVSVCIYFLLFI
                     IAPLIELFYDIKGLTSVIRVFGLIIPLSAFSAIQHAYVERNMLFKKYFFSTLFGTVVS
                     GITGIVMAMYGMGVWALVFQYFTNTAVDIIVLLFTVNWRPKFEFSILSAKHMMSYGWK
                     ILVADFFGTFFEQLRSLIVGKVYSSSDLAFYNKGNQLPTLITTNVSSSIMTVLFPAIA
                     NISDNYERVYQITRRAVQVVAYIMFPLLFGLAAVSDNLVLILFTEKWLPASIFVKILS
                     IGYAISMIGSVSIQSLKAVGRSDVVLRLEFIKKPIYVLLLILGVNINVTAVAVTMAIY
                     NLYGALVNSNQLRKVIGYKFKEQIQDLFPMLLLSTVIFVVVNMLSNIVENHLFTMIIQ
                     IAIGTGLYITMSVLFKIEVFGYLKEMIVLRFKSK"
     gene            45562..46743
                     /locus_tag="IVN13_000477"
     CDS             45562..46743
                     /locus_tag="IVN13_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase"
                     /protein_id="HAP7010996.1"
                     /translation="MKKILKMIKRKLQMFCGKTKIIIATVLYGKNNIEAHKIVVDNFL
                     GKGYGGDCKYIIDELLNRKLSCDIVWLVTDVNASVPDGVRKVQYGTLQSLKEFLSAKI
                     WISNVRNTLKPTRRTGQVYLQIWHGAYPVKYIEGQQEDLLDKDYVKQAKRDGHDTTAI
                     LVNSLYMEEIFRKYFWMNNHVDYLRFGYLRNDYLLKNIDNKRKIEETRRKLGIKNGAY
                     VILYCPTFRDNFSSNGYIQNAKLFIENLERKYGSVVLLVRLHPNDKKNISFFEYNDKV
                     INGSTVSDGLDLVLASDALITDYSSIAFDGAVLNKPVYILEKDIDTYRTTRGLSEQYD
                     LWPFVKVNNEIDLVEAFISFDSNKYKKRLNAYYQSDISYDTGETAQKTVNWMLKQMSI
                     M"
     gene            47041..47328
                     /locus_tag="IVN13_000478"
     CDS             47041..47328
                     /locus_tag="IVN13_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7010997.1"
                     /translation="MDVWLSQIKEPYNFLNLILFGNASPFIGVGHLWYLFALLYAYIG
                     IYALKRITNKDRLKIAIFLSMVLYLIEVVDYYYGFTVPEYLYRNVFLKDDP"
     gene            47869..49736
                     /gene="glmS"
                     /locus_tag="IVN13_000479"
                     /pseudo
     CDS             47869..49736
                     /gene="glmS"
                     /locus_tag="IVN13_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005423434.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
BASE COUNT        16530 a         8178 c        10536 g        14501 t
ORIGIN      
        1 acaacctctg atacacggtg ttccagaagt aacttcttcg aaaaataagc cgaaatttca
       61 caaaaatttg aaaagcaatt ttcttaaatt ccttcttatt tcttgaagct gcccacttct
      121 gtcacaacct cctttgtgta aaagaatcag tttctattcg atttcttcta tcgtttcttg
      181 caaacgctgt tcatattttt tgtttccttt atataaatca ccaatcgtcc atgccaacag
      241 gagaaggaca agaacaatga ttacataagc gatttgatca gccaatgcta gttgagctga
      301 ggtggcttgc tcgccgaaga aactttcgat ttgacctggt aatcctcgta agttcaagta
      361 agtcagacta atcacagaga accagccaag tagttttaca acaaatgaat ttttgaatcg
      421 gctccccatc tccgcttggc tattggtcat catcagtaaa ggaatcatcg agaatggcaa
      481 tgcaaaagcc agaaaaactt gggattcatt catcaagtta ttcaaagcgg tatgttcttc
      541 gatcgttcct tttgtacttg taaacaaaac acaaatcaat acaggaatca cagaaagcaa
      601 tcgtgtgact agtcttctta gccaaatcgg gatacgcata tggatgaagc cttccatgat
      661 gacttgaccg gtcaacgtac cagtaatagt agagttctgg ccagaagcaa gaagtgctac
      721 agcaaataaa gtggacaaga taccagattt tgcgacagca atcaagatac cattgctcat
      781 agctgatgga tcagataaag cttcatacag accaaaaaag gaaggatctt ggacagaacc
      841 ggttttgaat acagcgacac ccatgatcaa tagcagtgca ttgactagaa aagccattga
      901 caactggata ttagaatccc aagtagaaaa gcgtaccgct tgtgctacat cttcttcatc
      961 ttggtgatcg atcttccgag tttgagaaac tgctgaatga agatataagt tatgcggcat
     1021 gactgttgca ccaataatcc ctaaagctcc agtcagcgga gtttcaccgc cgatgaccgg
     1081 tgagtctgag aatgtatgac tgttagggat aaagccggca aacaaactgt gccaatcagg
     1141 gttagacaag ccaacttgat agacgaaaac gaataaaatg acgaaaatca agcagacaac
     1201 caaagcttcg attttacgaa acccgacttt tgtcagcaat aagaggacga aaacatctaa
     1261 cacagtgata aatacggcaa tcactaatgg aatatcaaaa agcaggtaga gggcgattgc
     1321 tgcaccaatg acttctgcga tgtctgttgc cataatggct aattcagtca agatccataa
     1381 aatgacaccc aatgtcttac ttgtccgtgc tcggattgct tgggccaaat ccatttgggt
     1441 cacgattcct aattttgctg ccatatattg taaaagcata gcaatcagac tagacatcaa
     1501 aataactgac atcaataaat attggaaatt ttgtccacca gtgatcgaag tggaccagtt
     1561 acctggatcc atataaccca ctgctactaa tgcacctggt ccagagtaag caagaagtgt
     1621 tttaaaaaaa ctttttccct tgggtacatc cactgttcca tttacttcag ctaaagataa
     1681 accatttgta tgttcaataa gtcgatgctt atgaaattgt ttgttggatt gattattcaa
     1741 gaaaaaacca cctttcctat ttatctacga ttacattcta cacctcagaa agaaaaaatt
     1801 aaagaaaaat attcggtatg cctaattttt tttcgtggcg tgtcttgctg gttaatgaaa
     1861 gaaattttgt tcttctctaa aaaaaccact attttttgca tgaaactaaa gaatacgcct
     1921 ttcttttttc aataaacttt agtaaaataa atcattgaga gcaattcgga aatagaaagg
     1981 agagatttat atggtatcaa caggtattgt cgtacttatt gctattattg ctttgctaat
     2041 cggcgcagta ggtggctttt tcctagcgcg taaatatatg aaagactatt tagaaaaaaa
     2101 tcctcccgtt aatgaggaca tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc
     2161 tgagaagaaa attcgccaga tgatgcagca aatgaagaac caagggaaaa aataggttct
     2221 gacccaaaga ctttttgcgg gctgattttt tcagtccgtt ttttatttcg taatttgtta
     2281 gactattcag actattcagc aaaaaagaca agcacagaca atcctatgct cttgataaag
     2341 acaaaggagg agtaaaatgt cgatttttag aaaattaggt tggttttttc gacaggaaaa
     2401 gaaacattat ttaattggga ttttctccct attcgcagtg gccttggtac agttagttcc
     2461 tccaaaggtg attgggatca tcattgatga gattgccgat aaaaatttat caataaagat
     2521 catttcctgg tggatcttat tattgatcct tgcagctctc ttacaatatc tttttcgtta
     2581 tatctggcgg acgaatattt ggggaagtgc agctcggttg gagaaagaac ttcggcagca
     2641 gctattcaaa cattttacag aaatggacaa tctcttttac caaaaatatc ggactggaga
     2701 tttgatggct catgcaacga atgatttgaa tgcaatccaa aatgtagcag gagctggtat
     2761 cttgaccttt gcagattcag tgatcacggg cggaacaacg atcattgcga tgattttgtt
     2821 tattgattgg cgcttaacac tgattgcgct gattcctctg cctttactag ccgttacttc
     2881 acgagtgctg gggtcaaaac tccacgatgc ttttcgtgat tcgcaagaag ctttttctag
     2941 tatcaatgat aagacacaag aaagtattac tggaatcaaa gtcattaaaa catttggaca
     3001 agaaaaagaa gatctcgcgg attttgaaga aaagattgaa gatgcaatta ttaaaaacaa
     3061 acgagtcaat tttttagact ccttatttga tccattcatt accttgatta tcggtctgtc
     3121 ttatgttgca acaattattt taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg
     3181 gcagatggtc tcatttatta gctatatcgg tatgcttgtt tggccgatgt ttgcgattgg
     3241 tcgattgttc aacgtattgg aaagaggaaa tgcttcttat gatcgcgtca atgaactttt
     3301 gcatgaaaag acgcatatcg tggaacaact gggtgcagtg cagaccccgg caaaaggcga
     3361 gctgaatgta gctatttcct cttttaacta tccgaaagaa gaatcaccag cattagaaca
     3421 aatcaaattt gtgatccaag aaggagaaac attaggtatt gttgggaaaa ctggtgcggg
     3481 aaaaacgacg atcatcaaat tactgatgcg tgaatacgat gattatcaag gaaaaatcca
     3541 atttggcgga cgttcgatca aagaatattc attgaatgct ttattaggat caatcggcta
     3601 tgtgcctcaa gatcacttct tgttttcgat gaccattcga gataatatcc gtttttctaa
     3661 tccaaaactt tcagaagaag aagtagagca tgcggcttct ttagcattta tcaacaatga
     3721 aatcaaggaa ttgccaaaag gttatgatac cttagttgga gaacgtggag tctctttatc
     3781 tggcggacag aaacaaagga tttcgattgc tcgagcattg atcaaaaatc ctgaattgct
     3841 gatcttagat gatgcacttt ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa
     3901 tttaaaagaa ataagaaaag agaaaacaac catcattgca gctcaccgtt taagcagcgt
     3961 gatgcatgca aaagaaatca ttgttgtcga tcaagggaaa atcattgaac gaggaaccca
     4021 tcatgagctg cttgcattag gcggctggta tgaacgaatg tgggagaaac agcaattaga
     4081 agcgaagata gaaggaggcg aggcctgatg gatgaaaatt atcaatcgga atggacaaaa
     4141 tcgattccac ttaaagaaca gaagaagatc atcaaacgac tgatgaagtt cgccaaacct
     4201 tttagaagaa cgttcatcgt tgctatctta tttgcgtttg ctctgtctgt gatcaatgtc
     4261 ctattgccaa ggatcatcca aacttttatg gatgatcatc tggctaaaca gtcggctacg
     4321 acacaagtta ttttgttttt tgctggtatg tacttgtttg gagtcatagt caaaagtatc
     4381 atttggtttt tccaatggta tttgtattcc atggcttcat tgaagaccta tcagtatgtt
     4441 cgggtaagat tatttgaaaa gctgcataca ctaggtatgc gttattttga tcagacaccg
     4501 gcaggatcga ccgtttctcg agtgacaaat gacacagaaa cgctatttga attttggtat
     4561 gtctttttga tggtattgac tgggatcttt gcagtcgttt cttccttcat tgccatgttt
     4621 caaatcaatg gagaaattgc cttgtggact ttggtgttct tgccgatttt aggaatcgtc
     4681 atctggtatt accagaaatt cagttctcgt atttaccgga atatgcggga aaaattaagt
     4741 cagctgaaca cgaaattgaa tgaatcgatt tccggcatgc gtattatcca gcagtttcgc
     4801 caagaagctc gtttagctaa agaatttgaa caagtgaaca acgagtattt agacacgcgc
     4861 tatgcgatga tcaaaacgaa ttcgattctg ttaagtccga tcatcaattt tctatatgca
     4921 ttggcgattg cgttggcgtt gactttgttt gggatcgatg cgctgcattc tcccgtagaa
     4981 gttggactga tctatgcatt tacgacctat gtccagggat tttttaatcc aatgtctcaa
     5041 atgatggact ttttgagcac ctttacagat ggaatggttg ccggtagtcg tattttgaaa
     5101 atcatggata cagaagaata tgcaccgcaa caaaatcctg gagcagatca aacgattact
     5161 tcaggtaaga tcgagtttcg caatgtaagc ttttcttatg atggagagca caatgtattg
     5221 aacaatatct cttttgtcgc taatccggga gagaccgttg cattagtggg gcacaccgga
     5281 agcggaaaaa gctcgatcat caacgtattg atgcggtttt acgaatttta tgaaggccag
     5341 atcttgattg atgatcatga cattcgagaa tatccaatgg aagagttacg taagaaaatg
     5401 ggattggtct tgcaagatgc cttcttgttt tatggagata tcgcaggaaa tatccggatg
     5461 atgaatccaa ctatcacaga cgaacagatt cgccatgcag ctgagtttgt tcaagcaaac
     5521 aagttcatcg atgaattacc aaaaggatat cactcaaaag tgatcgaaca cggtgccagc
     5581 tattctagcg gacaacgaca gctgatcact tttgcgcgaa caatcgtgac agatcctaaa
     5641 atactagtat tggatgaagc gacagcaaat atcgatactg aaacagaagg attgatccaa
     5701 gaaggattga caaaaatgcg acaaggacgg acaacgattg cgattgccca tcggctgtcc
     5761 acgattcggg atgcagaatt gattttagtt ttagacaaag gtcatatagt agaacgcggg
     5821 aatcatgaaa cacttctagc gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc
     5881 agtggtgtgt aaaaagtggt tgtgacaggg ctccgttcag ctttaggtga taagaaaaaa
     5941 attgtaaaaa aggctttcca gatttttgca taaatttggc ttatcaccga taagctgata
     6001 ctggaacacc gtatattaga agttgggaca gtagcatagt agcattttga cctaagcgct
     6061 agagtgaaaa taaaacagag cttcccagat tcttcatctg tgagcggtag tgtcgtagtc
     6121 accagattta aaaaagattc gacagctagt cagaagtgcc taatagccac taagaaagcg
     6181 ttgacaaaag cgcttgcttt cgataatata aagaaaacga tacaacttgg atagtgattg
     6241 cctgatgcaa gctaaacctg atgagtctcc catggttcca tggacggctt tttgggtttt
     6301 tctttttgtt ttggatacag ccctatatat catggaaagg agtaaaacat gctgattgag
     6361 aaagtactga ataacaacgt cgtaataacc agagatgaaa gaaatcagga aatgatcgtg
     6421 atgggaaaag gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc
     6481 gacaaaaaat ttcagttgac aaatgacgga ctttcagaac agttccaaga attggcacaa
     6541 gcgattcctc ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggct
     6601 ctacaagtcc agttgaatga ttcagtgtat attgcattga cggatcattt gtatacggct
     6661 atcgaacgtc acaaaatggg aatatttgta ccaaatgtat tgctgtatga tatcaaacaa
     6721 ctatttccta aagagtatgc catcggggaa aaaacagtga agaaaatctc ggataaatac
     6781 cacatcaagt tatcagaaga tgaagccgga tttatcgcat tgcatttagt caatgcacaa
     6841 ggcattcaaa gccaacaaga caatacctac ctgtttacta caacaataca ggatattcta
     6901 aatatcgtcc gctattacta taagattaca tttgatgaag attcagtcca tttttttcga
     6961 ttcgtcacac acctacgttt ttttctgaca cgcgtcctag atggagaaaa atcagaagtg
     7021 ggagaagttt ctgaggaatt gtaccaagtt attcaaaaaa aatataagga tacgtttggt
     7081 tgcgttgaga aaatagcaga ctttttagcc gagatattca actaccagat gtccacggac
     7141 gaaaaaatgt atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa
     7201 ataaaaaagg aggataaatt aacaagaacg catgaaataa gggtagtgac attcagttgg
     7261 ctaaaccttc cactaataaa ttggagggaa tagaaatggg aaaatatcaa gaattagcga
     7321 gcaaaattgt agaaaatgtc ggcgggaagg aaaatatcaa tggtttgaca cattgtatca
     7381 caaggcttcg tttcaaatta aaaaacgaag aaaaagcaaa tgatgagatc ttgaaaaata
     7441 tggacggtgt tgtgacggta atgcgagcgg gaggacagta ccaagttgtc atcgggaatc
     7501 atgtgccagt cgtgtttgaa gaggttataa gagcaggaaa ccttactttt gatgaggctg
     7561 tttcaacaga aaaaatgaga ccgtttgata tgcttattga tggaatatcg ggctgtttcc
     7621 agccattcct tgctattcta gctgctggtg ggatgattcg tggactgaca gcattacttg
     7681 tcttcttagg cgcatttgac cgcggttctg gcacatttgt tatgtttgat aatatcggtg
     7741 attctgtatt ccaatttatg cctgttatta tcggtttaac agctgcccgt aaatttaaag
     7801 taaatgaatt cgtagggatg ttgcttgggg cagcattaat gaatcctagc ttgtcgctgg
     7861 aagctttgag tggtgcagca gaagcaccac tgacgaccat attttctggc actatttttg
     7921 aagcacctat ttatcaaaca gtattcggca ttccctggat tgcgcgtaac tacgcttctt
     7981 ctgtcattcc cattatcttt attgtgttgt tagctagcca aattcagaaa ccaattaaaa
     8041 agttagtacc tgagatgatc gcgaatttct tcgttccatt cttcaccgtt cttattacaa
     8101 tgcccttagg ttttttactc gttggaccag cattcacatt cgcaactgat attttaatgg
     8161 ctggatttga aacactttta gcactttcgc cagttattta tggagcaatc gtaggcttct
     8221 tctggcaaat tctaatgatg tttggtctcc attgggcaat cgtaccaatg ggattgatgc
     8281 aattttctgt gaatggctgg caaaatatca tgacacctgt tgcagtagtg tcttttggac
     8341 aaactgcagc acttactgca ctctacttta agcttagaaa tccaaaagac aaagcaattg
     8401 caataccagc cattgtttct ggtattgtgg ggattacaga gccagctatt tatgggttta
     8461 cattgccacg taaaaaaata tttattttta cctgtgtcgg tggtgcgtta gggggcgcgt
     8521 atagtgggtt aatgaattta acttcttgga atcaaggtgg actaggcatc tttacaattc
     8581 ccaactatat tagaccagat ggtaatttaa cagatgtgat taatgtattg attgggatcg
     8641 ccattgcgat ggtggtatcc tttgcgctga cttttttctt ttggaaggag gaagcagatg
     8701 aaacagatga tagacagaaa acatcaggta aagaaattgt aaaaacaccg atacaaggac
     8761 aaatcgctcc actgaatgca gcaaaggatg cagcttttgc acaaggaaca ttaggtcgag
     8821 gaatcttgat ctaccctgaa aaaggagaag tacgtgcccc ttttgacgga acagtcatga
     8881 ccctgttccc gacgaaacat gcaatcggaa tggtttctga agcaggtctg gaattgctga
     8941 ttcatgtcgg attggatact gttcagttag aaggaaaata ttttaaaagc ttggtgcagc
     9001 aaggagaaag agtgaaacaa ggcgatctgt tgctccgctt cgacaagaag aaaatcgaag
     9061 aggaaggata cagtttagaa acacctgtga tcgtcaccaa ttacacggat tatttggata
     9121 ttttggaaaa tcaaggggag acggtcgggc cagacgatgc acttatcacc gcactgacct
     9181 gaacggttag gtgaaagaat ggactaggct taattattct ttttagtcca cttctttatt
     9241 ttcaaaattg aaaggctctt tcatgatggc tgagctgata gggcgtttat caaatttttg
     9301 tcctacagaa attagatgga ctaaattctg ttgataattt tctgcaaaaa cacaactata
     9361 taacacgtct agaagataac aaatcgatgt attgatcgta aaattaccaa ttttcgaata
     9421 cagtcgttcg cgtgtcgtaa gtcgcaaagc ggctgtactc attgaagata gcgttgaatc
     9481 accgatactg gtcaaagcta aaattggcac atgattttgt ttcaatattt ctgcggtacg
     9541 aaggatcaca ttgttttcac ccgtataaga aatcagaatc ccgcacatat cactcgtgca
     9601 attgtacgct tcatagaacg attcaccaaa agtggtgaca atcgtgacat ccattttgat
     9661 gcgcttcatt ttcagcatga aatcttgaga aatcaaagta ttcgagttac tggcgaaaat
     9721 cacgattttt tttgcttgaa gcattagttg tttggctttt tgcagttggt catgatgaat
     9781 gagagaaagt gtatcttgaa tcgtcatctg ttctaaagaa gccattttat ttgcgatcgt
     9841 cataataccg tctgtggtgg aaaaaggaag attcgcatct acgtcttgaa aattcccttc
     9901 aagataggct gtttcagcta aaaaagcatt acgaaaactg acccagccat caaaacccat
     9961 tttttttgct acacggatga gtgtggacgg gtggacataa catcgctcag caatctcttg
    10021 gatggtcatt tcgccaagtg ctggagaatc gcttaagaga aactgaatca cttcattttc
    10081 tgaaggagaa aaggagattt ctttgatttt ttcacgtaat aacatgtaga taccttcttc
    10141 ttgttttttt acgtaaatta taactttttt gaaaatgatt tgtaaacgga tttatgttat
    10201 atagcttaat aaagacattt tttgacaaga tatttacaaa aatccagaag aaaacactaa
    10261 cttttgtaaa tgttactgga aagaaacgaa tttctattgt ttgttttgtt tacaaaaaag
    10321 ataataaaag tgtaaaaagt aaaggagtga gaacatggca aaaggaatca aaatcgctac
    10381 gattggtgga ggcagcagtt acacccccga gttgatggaa ggatttatca aaagatatga
    10441 ggaattaccg atacgagaaa tctggctcgt agatgtagaa gaaggaaaag aaaagcttga
    10501 gatcgtcagt cagatgtctc agcgtatgtg ggatgcttct ccgtatgaag tcaaaatcta
    10561 tgcaactctg gatcgggaag aagcattgaa agatgcggat tttgttacga ctcagtttcg
    10621 tgtcggtcaa ttagatgcac gaatcaaaga tgagcggatc cctagttatt atgggatgat
    10681 tgggcaagaa acaaatggtg ctggcgggat attcaaagct tttcgtaccg ttccagtggt
    10741 tttggcaatc gttgaagaca tgaagcgttt atgtccaaat gcttggctga tcaatttcgc
    10801 gaacccaagc gggatggtga cagaagcagt tatcagatat ggcaaatggg ataaagtgat
    10861 cggattatgc aatgtgccag taatggcgat gatgacagaa ccaacaatgc ttggcaagaa
    10921 aaaagaagag ctgatctaca agtttgccgg tctaaatcac ttccattggc acaaagtagc
    10981 agataataaa ggaaatgacc gcacggcaga attgattgat cgtttatacg atgaagacaa
    11041 tggattgccg aaaaatatct ttgatatccc gtttttcaaa gaacaattaa aacaaatgaa
    11101 catgattcct tgtggctacc accgctatta ctaccgtgaa gaagaaatgc tggaacatgc
    11161 tttagaagaa tatcaaacga ttggtacaag agcgcagcaa gtgaaagaaa cagaagcaga
    11221 gctgtttgaa ttatacaaag atccagaatt ggatcataaa cctgaacaat tgcaaaaacg
    11281 aggtggtgct tattattcag atgctgcctg tgaaacgatt gcttcgatct atggaaataa
    11341 agaaacacag atcgtggtat ctacaaagaa tgatggagct gttcctgatc tgccggcaga
    11401 ttgtgtagta gaagtcactg cctatgttgg cgcacaaggc gcacgaaatg tagcgtttgg
    11461 ttcccttcct gctgctgaaa gaggctggtt gcaagtaatg aagaatatgg aattgctgac
    11521 gatcgaagca gccgtgactg gtgattatgg aacagctttg caagcgttta cgatcaatcc
    11581 gcttatacga tcaggaaaga cagcaaaacg gataatggat gagttgttta ttgcacatca
    11641 gcagtattta ccgcaattca aggaaacaat cagccggtta aaagcagaag gaatcgaagt
    11701 ccaagatgaa ctggcaagag aattatcaag caatacacat gtaatggagg aagtatgatg
    11761 ggattcagaa aagatttttt atggggcgga gctacagcag ccaatcaatg cgagggcggt
    11821 tatgatgaag gcggacgtgg gttagcgaac gtagacgttg tgccaatcgg agaagaccgt
    11881 tttcctgtca ttactggaaa aaagaaaatg tttgaatttg atgaaaatca tttttatcca
    11941 gcaaaaattg cgatcgatat gtatcatcat tataaggaag atattgcttt atttgcagaa
    12001 atggggttca agacttatcg gatgtccatt gcttggagtc gtatttttcc aatgggcgat
    12061 gaaaaagagc caaatgaatc tgggttgaaa ttttatgagg atgtctttaa agaatgtcaa
    12121 aagtacggga tcgagccgct tgtgacgatt acacattttg attgtccgat gcatttgatc
    12181 aaagaatatg gtgcgtggcg ttccagagaa ctcgttggtt tttatgaaaa tctgtgccga
    12241 gtcatcttca atcgctataa aggacttgtc aaatattggt tgacattcaa tgagatcaat
    12301 atgatccttc atgcaccatt catgggcgca gggctgtatt ttgaagaagg agaaaatgaa
    12361 gaacaaatca aatatcaagc ggcacatcat gagttggttg caagtgccat cgcaacgaag
    12421 attgctcatg aagtagatcc ggagaacaaa gtaggctgta tgctggcagc aggagcatat
    12481 tatccataca cttgcaatcc tgaagatgtt tgggcttcta gaaaatcaga tcgagaaaat
    12541 tatttcttca ttgatgtcca gtcacgagga gaatatcccg cttatgcatt gaaagaaatg
    12601 gaacgaaatg gcattcagat cgaaatggaa cctggggatg ccgcattgct gaaagaacat
    12661 actgttgatt ttatctcctt ttcttattat tcttctcgag tttcgactac tgacaacgaa
    12721 ctcttggaac agactgcagg aaatatcttt gcttctgtca aaaatccata tctggaagca
    12781 agtgaatggg gttggcaaat tgatccatta ggcttgagaa taacgatgaa tgatatttat
    12841 gatcgttatc aaaaaccact atttattgta gaaaacggct taggtgcagt cgatacgccc
    12901 gatgagaatg gttatgtgga agatgattat cgaatcgact atctggcagc acatatccaa
    12961 gcaatgaaag atgcagtaga attagatgga gtcgatctat taggctatac gacttgggga
    13021 tgtattgatt tagtatcagc tggtacaggt gagatgaaga aaaggtacgg attcatttat
    13081 gtcgatcgtg acaacgaggg aaatggcaca ttagcccgtt cgaagaagaa atcattcaac
    13141 tggtataaaa aagtaattga ttcaaatgga gaaaatcttt cgaatgattg atcaatgatt
    13201 tataaaagtc tattgttttt cagcggattt ttcgagtata ataaagctat tcgagaaacg
    13261 caagcagaac aatagacttt ttttcgcatt ggctgtaaag tatgcttact tgattaagta
    13321 aaggagaatc tacatatgta tgatgtaatc gttattggcg caggtcctgc tgggatgact
    13381 gctgctttat atgcttcacg ttcaaactta tccgtattga tgatcgaaca aggcgcccct
    13441 ggcggccaaa tgaacaatac ggcagaaatc gaaaactatc ctggtttcga ttcaatcatg
    13501 ggtcctgatc tagcttataa gatgtatgaa ggtgtttcaa gatttgggac agaaaatgct
    13561 tatggaatcg tgcaagatat caaggatcac ggcagccaca aagaagttat ttgtgaagat
    13621 aaaacttacg aaggaaaaac agtcattatc gcaacaggct gtatccaccg taaattaggc
    13681 gtacctggcg aggaagaatt tgctggacgt ggcgtttctt actgtgcagt ttgtgatggt
    13741 gcgtttttcc gtaacaaacg tctagtagtg atcggaggag gagactcagc agtagaagaa
    13801 gccatctatt tgacacaatt tgcttctgaa gtagtcatcg tccaccgtag agatgaatta
    13861 cgtgcacaaa agatcatcca agaccgtgca tttgcgaatg aaaaaatttc ctttatgtgg
    13921 gatacagtcg ttgacgaaat cgttgggaat gaaatggtag tgacaggtgt gaaagcacgt
    13981 aatgtcaaaa ccggcaatga gtttgatttg gcagcagacg gagtattcat ctatgttgga
    14041 ctggatccat taactgcacc atttagagaa gcagggttga cgaacgaagc tggctggatt
    14101 ccaacagaca acgaaatgaa gacgaatata ccaggcgttt ttgccatcgg agatgttcgt
    14161 gaaaaagatt tgcgacaaat cactactgct gttggtgatg gtgggatcgc tggacaacaa
    14221 gtgtataaat acttggaatc tttagaagaa acaactgcat aagtttaaaa agagtggaag
    14281 aacggtttca attgttcttt tactcttttt tttcgcgtga aatctactga atccgattta
    14341 tgcgaatact tgcaaccgtg cttgataaaa gaatcctttc ttgatttaag cgttttttgg
    14401 tcctcatact tttatatcta ataagtgaat gctatactta ttaagaacca tatttcagaa
    14461 agaggtgtta ttttcatgtc ttgggaacaa gtgtatgaac aatgggtaca tgaagaaaat
    14521 ttagaagaaa acttaaaaaa acaattaaat gatctgggtg aagatcctga aaaacttgaa
    14581 gatgcttttt atgcaccgtt ggaattcggt actgcaggca tgcggggaat cttagggcct
    14641 gggatcaatc gaatgaacat ctatacggta agacaagcaa cagaaggact tgcacgtttc
    14701 atggatacac aaggcgaaga aacaaaacgc cgaggtgtcg ctatcgctta tgactcacgc
    14761 catcaatcac cagaatttgc catggaagca gccaaaacat tagcacatca caatatccca
    14821 tcttatgtat tcgaaagtct acgtccaaca ccagagcttt cttttgcagt ccgtcatttg
    14881 aagacgtttg cgggaatcat ggtaactgca tcccacaatc cagcagctta caacggatat
    14941 aaagtctacg gcgaagatgg gggacaaatg cctcctgcag acgcagatgc attgacgaaa
    15001 tatgtccgtg aagtcagcaa tccgttaaaa gttgaagtat tatctgacga agaagctgaa
    15061 catagcggat tgatcacgat catcggagaa gaagtggatg ccgcttatct agaagaaatc
    15121 aaagcagtaa cgattgatca tgaattggtg gaaacaatgg ggaaagattt gaaacttgtg
    15181 tacacaccac ttcacggaac aggaaaaatg ttgggcgaac gtgcgttgaa acaagctgga
    15241 ttcgaacaat tcgtactagt tccagaacaa gctgtcgcag acccagattt cactacagtc
    15301 aaatcaccaa atccagaaga acactctgct tttgagtatg cgatacgact tggcgagaaa
    15361 gaaggcgctg atttgctgat tgcaacagat cctgatgcag accgtttagg tgcagccgtt
    15421 cgtttgccaa atggctccta tgaagtattg accggaaacc aaatcggagc actgtttgct
    15481 caatatattt tggaagcaca ccaatcgcga ggcactttac ctgaaaacgc agcggtattg
    15541 aaatcgatcg tttctagcga attacctaca gcaattgcgg aacactatgg ggctaccatg
    15601 ttcaatgtat tgacaggatt caaattcatt gctgaaaaaa ttcaacaatt tgaagaagat
    15661 cattcccata cttttatgtt cggattcgaa gaaagttacg gttatttagt taaacctttc
    15721 gttcgcgaca aagacgcgat ccaagcattg ctcttattag cagaagtagc agcttactat
    15781 aaaaaacaag gcaaaacgtt gtatgacggc ttgcaaacga tttttgaaac ctatggctac
    15841 tacgcagaaa aaacgatttc tgtgacaatg agcggacaag aaggttctgc gaaaatctct
    15901 gctttgatgg aaaaattccg tgaagaagca ccaacagaat ttgccggagc aaaagtcatc
    15961 caaacagaag acttcaaaga gttgacgaga acagaccgtg aaggaaacgt agaaaaacta
    16021 acgacaccgc catctgatgt attgaagtat acgctggaag aaaatggctg gatcgctatc
    16081 cgtccttcag gaactgagcc gaaaatcaaa ttttacatcg gtgtaaaagg aaactcttca
    16141 gaagacgcag ctgaaaaagt agccgcttat gaagcgatta tccaaaagat gacagaagaa
    16201 taaaaaaact tgagactgat ggaaaaagtg gtaaacttta gaagtcagtc ttttttcaag
    16261 ttggaggtga atggattgcc agacacgcaa aaaacaaaag tagagaaagt cagccaacta
    16321 ttcaaaatgt tgagtgaccc cactcgtctc aaaattctgc tgtatctgaa agacggggaa
    16381 caaaatgtga cagcaatcac ccaagcagtg gaaatggaac aatctgctgt atcgcatcaa
    16441 ctgcgtttat taagagaaaa ccatgtcgtc aagtcccatc gtgagggaaa agcgattttg
    16501 tatagtcttg atgattctca cgtactggat attttgaacc agacactcaa acatgtggaa
    16561 catcaataac gataggtgca aatagagaca aactggactg tgtcaacgaa tcttcttcgc
    16621 tgacacagtc cagttttttt taagaaaaag acaagaaaaa ctgttagtct gtttcttcgg
    16681 tggtttcagt tccataataa ctatgatctt ctaggtctaa agcattcaaa ataagcgaag
    16741 ctgtttcttc aatagataaa gaagcgacat tgattactac gcagcccagt ttttcataaa
    16801 gatcatttgc aaaatcgagt tcagctcgta ttttttcgat atcagaataa gcagtatcag
    16861 gatttaatcc gtaagagcgc atccgttctt tacggatatt gtttaaaata tctgggttat
    16921 tcgtcaatcc cacgattttt ttaggatcaa tctcccaaag ctgtttggga atatgtgctt
    16981 gtgggatcaa aggaagattg gcaactttta aatttttgtt agctaaaaac aagctcaaag
    17041 gcgttttgga agttcgggat acgcctagca aaacgacatc tgcttctaga aacccacgag
    17101 gatctttccc gtcatcgtat tttacagcaa attccattgc tttaatccgt ttgaaatagt
    17161 tctcattcaa taaatgaagg gcgccaggtt gccgaatggg cttgatacca gttaagcgct
    17221 cgatttctga tatcggcgga gtcatcacat caaaactaaa taattgatgt tcttcacaaa
    17281 aagaacgcgt catctcgatc aattcttgat tgatcaatgt gtgtaagaca agagcttttt
    17341 cttctagtgc atcttgcagt gcctgcatca atgttttctc atctgtcaca aatgttctgc
    17401 ggaataaatt gaactcaacg ctaggatatt gggccatagt ggcttgcgct aattttgtcg
    17461 cagtttctcc agcagagtcg gagatgacaa aaaaagtaac gatttctgta gaatcggaag
    17521 acatggataa aacctcactt gcattttttt tatgtttatt ataccatact ctagaatgtg
    17581 tacttcgtaa tcattttgat ttggaggaat aagtcatgaa aaaacagaca ttgatcgaac
    17641 aatcactaga aacattgaaa aataacggtt ttaaatatac taaaaaaaga gaagcattgt
    17701 taacttattt agtgaagcgc aatcgctacg tatccgctaa agaagttttt gactatatga
    17761 atcaggaatt caaaggagta agttacgata cgatttatcg aaatctccat gattttgcag
    17821 aaatcgattt actggaagaa accgatttaa atggagagat gaagtttcgt tttcattgct
    17881 gtcaagggga aatcggccat caccatcact ttatctgtac ggtgtgcgga acaacgaaag
    17941 aaatccatat gtgtccaatg aattatttcg aagaacagct cggtggatgt acgatcgaag
    18001 ggcatagatt cgagattttc ggaagatgtg aaaattgtca ataaggattt aaataaaata
    18061 acatttgata attttaaaaa aaagcaagat ttccttgacg gtcaaaacag cattagatat
    18121 aatgtttaca ggacagtctt gttttttgag atatatttca ataacaatta cggttacttt
    18181 ttaagctcgg agggagggaa ttaacatgtc aaaaacagtc gttcgcaaaa acgaatcact
    18241 tgacgatgct cttcgtcgct tcaaacgttc cgtgtcaaaa gcgggtactt tacaagaatc
    18301 tcgtaaacgt gaattctacg aaaaaccaag tgtaagacgc aagaaaaaat ctgaagcagc
    18361 tagaaaacgc aaaaaattct agttctgtga tgaattggag ttggtagtgt gtcactacta
    18421 agtagattaa acgatgacat gaaaacagcg atgaaagcga aagataagga atctttacaa
    18481 gtcatccgaa tgatcaagtc atctatacaa aacgagcaga ttaaagaagg tcacgacttg
    18541 acagaagaag aagaattaac ggtgttgtct cgcgaaatga aacagcgacg tgattctttg
    18601 catgagtttg aagaagcagg acgtgacgat ttagctgaaa aagtaaaaag tgaaattgtg
    18661 atagtcgaaa aatacatgcc ggaacaactt tccgacgaag aaattcgaca acttgttcaa
    18721 gaagcaatca cacaaactgg tgcttcatca atgaaagaat ttggcaaagt catgggtgcg
    18781 atcatgccta aagtcaaagg aaaagcagat ggaaaccaag taaatgccat tgtgaaagaa
    18841 ctattacaat cttaatttta tcatttaaac tgcggacaat cattgtccgc agtttttttg
    18901 tgctcgtaaa aaagaaatct aaaaaatagc tagacgaaag aataggattc tttcatctag
    18961 ctggtttttt cctgtttaat atttcttatg ttaaattgtg tttgatctgg ttattcttct
    19021 ggtagttcat ttgcttcttg tttgtaccct gataggcgat aggcgtaata gaagagtaag
    19081 ctgccagcgt aaccaagtaa cgttgggacc atccagccaa gtcctaatgc agcaaaagga
    19141 acgtgatgat gataaaattc gagcatgctt gctacaactc tttgttgact cactaaagca
    19201 ggcgaatttg ccagcatatc caatacggcg ggaacagcag caaacaacat cgttacttga
    19261 tagacaaatg gtgtcttttg gaaaaatttt gctgttaagg aaagcaagat caacgcaagc
    19321 gacaatgggt acaaaagcat caatactggt aaagaccatt ggatgatatt gtccaaacca
    19381 gcatttgcaa cgacaaaaga gacgaaagtc gttaaccgca gccatgtcat ataactgact
    19441 ttagggaaca gtttatggaa atcttgtgca aaagaaacaa cgagtcccat agcggtagtg
    19501 aagacaccta atgtgaccat cactcctaag aagctagcgc cgaagttacc taaataaaaa
    19561 ttagtaatct gagaaagtgc agttccgcca ttttctgata aagacatctt attcaaactc
    19621 atcgcaccga gaagaacaag tccaaaatag atcatgactt ctagcgcaac gctcaggcta
    19681 ccagcttttg ctgttaattc tgtgactttt ttatctttga aaccaaggtc acggactgca
    19741 tggacaaaag tgacacttaa ggcaagcaaa gctactgcat caaccgtgtt ataaccttct
    19801 aggacaccat ttgttaaagc gtttgtttgg taagtatctg cagcagattg atttagattt
    19861 cccattgggt ggaataaaga aacgacaaaa atgattgcta ataatgcaag aaacaaggag
    19921 tttagatact ttccgacgta tttgaccaac cgtgtgggat ggacagtaag gaaataagca
    19981 agtaaaaaga aaagagcaga gaataaaaac atacctaatg aattatattt ttcaggcaaa
    20041 aatggtttag cagccatttc aaaagcagta gcagctgtcc gtggtgtacc gaaaaatggt
    20101 ccgatcgtca aatgaacaag gattaagaac aaggcagcat accattttcc gacaggttta
    20161 gctaagtcat acagaccatc actttttgtc actactacag ctaagatagc tagtaatggg
    20221 aaaagagcgc ctgagatggc aaatcctacg ccagcactga cccagtttgt gccagccatt
    20281 tgacctaagt ggacaggaaa gattagatta cctgacccga agaacatccc gaagataagt
    20341 gaactgacca tcaatagttg tttccatgaa taagtttttg tttcttccat aaatttttcc
    20401 tccaataatt atcattttga ttgacgatat aaacgttgca gcaattagta atgccattaa
    20461 tatcagattc catgaaaacc accaccttaa aatataaaaa aagccctttt ccacttggga
    20521 aaagggctct agattctaga acggtaccac cttttttcgc ttaaaaaagc ttcttcattc
    20581 acggacgctt tttgataaag ttatccgcta gcctgataat ggaggcagcc acaatgatct
    20641 acgttttcga cattgactcg gaaatgatgt tcgtctattc gatttattgt catttctcac
    20701 ttacatgact cactgggaat aaatcgttga atagcctact gttttcctcg acgctttgct
    20761 gtatgtttat ttgttgacgt aataatacac ctgttttttt tgaaatgcaa gaatatttta
    20821 actattttgt tttctttttt tgaaacaaga aaaataccta gagaatacct gctgaatgaa
    20881 acggaatatt ttgttctcca ctttcatata acttacattc tcagattctt tttatcaaaa
    20941 cgagaccttt agcgaatgga aacggctatt ttttaacgtt tcttggtacg tatggttaaa
    21001 cgactctttt acaacctttt cttatttgct atatagtttc ttctgagtat ggtaaaatag
    21061 gaaagaatga atcgaaaaaa aggggatgtc tatttttgac ggaagagtct agttctttag
    21121 agattagatt gacagctgct gatgatgtca gcatgctttt aggtgcacac gacaagcata
    21181 taaaactgat cgaggaaaca acggaaacaa ccatccatac acgtggagaa atcattcaga
    21241 tcattggtga acaatcccag atttccttgg ctgcctcagt cattcggaca ttacaggaat
    21301 tgatcaaacg aggaatccac gtaagtacgc ccgatgttgt gacggcattg aaaatggggc
    21361 aaaaaggtac attagattat tttgttgaga tgtatgagga agagatcgtc aaagaccgta
    21421 atggaaaacc tatacgggta aaaaacagcg gtcaaaagaa atatgttgaa tcggtagcca
    21481 aacatgacat cgtatttggt gtagggccag ccggaacagg gaaaacgttt cttgccgtag
    21541 tattagcaat tgctgcctta aaaaaaggcg aagtgcaaaa aatcattttg acgcggcccg
    21601 ctgtagaggc aggggaaaac ctaggctttc taccagggga tctgaaagaa aaagtggatc
    21661 cttatctgcg gcctgtttat gatgctttat accaaatttt tggattggat catacgaacc
    21721 gattgatgga acgaggcgta atcgaaatcg caccattagc ttacatgcgg gggcggacat
    21781 tggatgatgc attcgttatc ttggacgaag cacaaaacac gacagtcgct cagatgaaaa
    21841 tgttcttgac ccgtctcggt tatcaatcaa aaatgattgt caatggggat acaagtcaga
    21901 tagacttgcc tcgagggacg acaagtggtc ttgttcatgc agaacgaaca ctaaagcaaa
    21961 tcaaaaaaat cgattttgtt aattttgaag caagtgacgt tgttcggcac cctgtagttg
    22021 ccgaaatcat caaagcttat gaaaaggcgg atcttcatca agaataagct ttgcttagga
    22081 ggataagcac atgttgaata aaacactgca gaaactacaa gaaaaactcg gaaaggcagc
    22141 attgcctttc cttctgttct tgttttttat cattgtttgc ggcgttacat ttagtagtgt
    22201 caaacaaaag gccaatgatt ttagagaagg ccaagtagca gaagagagta tccgtgcgaa
    22261 taaaacgatc gagaacacgg aagaaaccga acaaagaaga aaactagcag cagaagcagt
    22321 gactccagaa tatacttatc aaaaagatct ggcagatgac cagaacaatc ggatcaagca
    22381 attattcgaa ttgatcgata agacgaatga taccatcaac aaatcttata atgacaaggt
    22441 agataaggcg aaggaagatg aaaatatccc gaaacctact acagaagagc ggattgctac
    22501 actgaagaaa aattttgaaa acgtgaatgc agaaaacgtt gcgttttacc aaaaactgcc
    22561 gaacaatttt tattcgacga tatttgaaat gactgaaacg gaaattcaca cggttaggga
    22621 tgaaagtttg aagctcattg acgaacagat gagtaaacaa gttcgtgaaa gcgaattgga
    22681 agcttttaaa caagaagcag aagatcagat tcagtatttg aatgtcacaa cagagcagca
    22741 acaaatgatc cgctacttag tggatcaagg cattgtagtc aatgatgtat tgaatgagaa
    22801 aaagacagaa gaattaaaac aatctgcacg agaagctgta caaccagtga tgatcttcca
    22861 aggagaaatc attgttcgcg aaggaaatca aattgatgct tcagcgatga aaaaattgga
    22921 attactagga ttgacgaatc aaacgacttc gattttccca ctcattgcca tggttttagc
    22981 tgttttgctt caaatcggtg tactgattta taattcactt caatttcaaa atgcaggaga
    23041 aagaacaaaa tacgtacttt tctacgtgac agcaatgtca gtcagcgtag tactaatgaa
    23101 atttttccaa ctcttccaaa cagaacaagc tgcttatatt ccgctgtttt accccgcggc
    23161 ttttgctcca cttgttttaa gctttttctt gaatcgaaga gcagggatca tggctggtat
    23221 tttccaagca gtatccgcac ttttcatttt ttatgattcg attggcacga actttctgac
    23281 agttattttg atgtcctatg tattttctgg tctcatggca acgatcgtca gacgcaaacg
    23341 gatctcagaa caagggttat tcgctgctat gtgggtcatt attttccctg tgatgatgga
    23401 tgtcattttg attatctacc aaggaatgag ctttggggat gcaacgactt ggttaacgct
    23461 agtctgcgga ttcacaggtg cattgttctc attcttgctg acgataggtc tgcaccccta
    23521 tattgaattg atggtcaatg atgatagtgt gatcgtatta aatgaactca gcaatcctaa
    23581 tcacccttta ttgaaacagt tgttagaaga agcaccaggt acgtatcatc atagcatgat
    23641 ggtagccaat ctaagtgcta atgctgtagc agaaatcggt ggacgttctt tactaactcg
    23701 agtagcttgc tattatcatg atatcggcaa gatcaaacac gccagctttt tcgttgagaa
    23761 cttgccttca ggagcggaaa atccgcacaa tttcttattg ccagaagaca gtaaacatat
    23821 tatctttgga catgtgacag atggtgccaa aatattggaa gattataata tgccgcaaat
    23881 ggtgatcgat atttgcagac agcatcatgg aaccacgctg atgcgttatt tctacgttaa
    23941 ggcaaaggaa cgcaacccgg aagtcacgga agagcaattc cgttatcctg gaccaaaacc
    24001 tcaaagccgt gaagcaggaa tcgtcaatat agctgacagt tgtgaagcgg cagtacgggc
    24061 aatggaccat ccaacaagcg aaaaaatcga aagttttgtc cataatctta tagaagagcg
    24121 tatttccgat ggacaattgg atgacagtgg attgacgtta aaagaaatcc gtaaagtaga
    24181 aaaatcattg atcagcggtt taagcagtac cttccattct cggatcaaat atcctaaaat
    24241 gaaatcggaa gttgagaaga taaaagaaga acaagaagaa tagcaagaag aatagaaaga
    24301 agcgatgtat aatggatatt accttcatgg atgaaacaaa ccaagtctca gaagagaaaa
    24361 tgaaagaaat cgacgacttg ttgcaatttg cagcatcata tttagagttg ccagaagaaa
    24421 cagaaatgtc tgttactttc atggacaacg cagcgatcca agtaatcaat cgtgattacc
    24481 gtggaaaaga tgcaccgaca gacgtcatca gttttgcctt ggaagaagag ggtgaagacg
    24541 aaatcccagt gatttttgaa gatggagaga atccattgcc tcgtgtactg ggagacatca
    24601 tgatctctac tgaacgtgct gcagagcaag cagaagctta cggccattcg tatgatcggg
    24661 aacttggctt tttagcggtt catggattct tgcatatcaa cgggtatgac catatgacac
    24721 cagaggatga gaaagaaatg tttggtttac agaaagagat cttagatgcc tatggactta
    24781 aaagataaaa acgtagagaa aaacaaacat ttcctgatgt ccgtggaatt tgccgtccaa
    24841 ggagtcaaaa ctgtttttga ggaagaacgc aacatgagga aacatgttgc gttcggaatc
    24901 ctcgctttgt ttatgggcgt gattttccaa cttgatcgga tagaatggtt atggctgctt
    24961 ttagctgttt ttctcgtatg gatagtagag atcatcaata ctgtttttga aaatgtcgta
    25021 gacatgttta cggattttca ttttcatccg attgggaaaa aaatcaaaga tatggcagct
    25081 ggagcagttt tagtgaccgc ttgttttgcc gtcatcgttg ggttgatttt atttgtccct
    25141 aaaatatatc atttgttgtt taattaaaaa ggagaacgta tatgccagaa cataaatcag
    25201 ggtttgtcgc aatcgtcggc cgcccgaatg taggaaaatc cacattattg aatcgaatcg
    25261 tgggtcaaaa aattgccatc atgagtgata aggcgcagac aactagaaat aaaatccaag
    25321 gagtatatac tacaccagaa gctcagttga tttttattga cacaccagga atccataaac
    25381 caaagcatcg tttaggagat tttatggtag aagcagctta cagtgcgttg aaagaagtgg
    25441 acgcggtctt atttatgatc agcgctgatc aaaaacgcgg ccggggagac gattttatta
    25501 ttgaacgtct aaaaaacgtg cattctcctg tttatctgat tatcaataaa atcgataaaa
    25561 tccatccaga cgagttgttg ggaatcatcg aagattattc aacgcagatg ccctttgcac
    25621 aagtgattcc aatttctgct acagaaggaa acaacgtcga acgtttgatg gatgtattag
    25681 tcagtgaaat gccagagggc ccacaatatt ttccagatga tcaagtgact gatcatcctg
    25741 aatacttcat cgtttctgaa ttgatccgtg agaaagtctt gtttttaaca cgagacgaag
    25801 tgcctcattc tgtcgcagtc gttgttgatt cgatgaagcg aaacgagaat gataaaatcc
    25861 atatccaggc cacgatcatc gtcgaaagag atagccaaaa aggaatcatt atcggcaaag
    25921 gcggaaaaat gctgaagcaa gtaggaacga aagcaagaaa ggatatcgaa aatcttttag
    25981 gagacaaagt atttctagaa ctttgggtca aagtccaaaa agactggcga gacaaaaaag
    26041 tctatctgca agacttcggc taccgaaaag atgaatactg atattctgaa cttcttatag
    26101 gttaagtata gaaataaact ggataaaaat ccgcacgttg agaaaaacgg cggaagatcg
    26161 aaacataaat aatctggatc ggcagaatct gcttttccag atttattttt taggagggag
    26221 aatatgagcc agcttgcaga atcgaaaggc ttgattttat ctagccgtaa ttacaaagaa
    26281 aaagataaat tagtcaaaat ctttacggaa tcttctggca aaatgatgtt ttatatcaaa
    26341 ggaatccatc ggcaaaatca gccattagct ccggctttgc tcccctttac cgaagcagtc
    26401 tatatcggta attttcgaag tgaaggtttg tcgtttttaa gcagtgtcaa agacgtccat
    26461 gcgtttcgga acatccaaga agatatcttt ttgagcggtt atggtaccta tttgctaaat
    26521 ctagtagatg cagcaatcga agatcatcaa tatgacccgc atttattcca atttacgcaa
    26581 caagctctgc agcggatgga tcaaggagat gatgcagaaa tcatcacgaa tatctttgaa
    26641 gttcaaatac tgcaacgctt cggaattgca ccaatctgga cacactgcgt cgtatgtggg
    26701 gaaacaaaag gaaaatttga ctattcttcc aaatatggtg gtgtgatatg cgaaagacat
    26761 tggtcgatgg acgatcaccg ttatcatgca gatcctcgag ctgtttattt cgttcggatg
    26821 ttttcagcta tttcttatga taaaatcagt ggaatccatc tgaaagaaga aaccaaacaa
    26881 gcaatccgtg aactcatcga ccagctgtat gatgaatatg tcggtattca tttgaaaagc
    26941 aaaaagttca tcgatcaaat gaagtcttgg gcacacgtat taaagacgcc agagaaagac
    27001 atgtctgaaa aagatctacc taaaaaagaa gaatagtaaa tgaaacatac aacaaaaaat
    27061 caaaaccatt tgacaaacac agggagttcc gtctattatt aaccgtagat caaatagatc
    27121 gttcagtgag aggatctatt atagaaacaa gaaaactgca aaatgaatac gtacattgac
    27181 caagatcagt aaaaaacacg tggtttagcg agttcgggag agtgggagcc ggacccaaac
    27241 gatttttgaa gaggcacttg cgagtacgat aaatgaagct gccgaatctt tattcggaag
    27301 tagggtggaa ccgcgaatta ttcgtcccta tgtctgtaat cagacatagg gattttttgt
    27361 cgttttcttt cctgtattct tatttgacag caacgcttta aaattgaaag gggcaaaaca
    27421 gatgagtaaa aaattgaccg tccaagatat gattttaaca ttacaaaaat tttggtcatc
    27481 aaacggctgc atgctgatgc aagcttatga tacagaaaaa ggagcaggta cgatgagccc
    27541 atatacattc ttacgggcaa tcggaccgga accatggaat gctgcttatg tagagccatc
    27601 ccgccgacca gctgacgggc gttatggaga aaacccaaac cgtttgtatc agcatcatca
    27661 atttcaagtc gtaatgaaac cttcacctga aaatatccaa gagctttatt tagaaagctt
    27721 gcgtctttta ggaattgatc cattagaaca cgatattcgt tttgttgaag acaactggga
    27781 gaacccatcg atggggtgtg ccggtgtggg ttgggaagta tggcttgatg ggatggagat
    27841 cacacaattc acgtacttcc agcaagtcgg aggactacct tgtaaaccag ttacttctga
    27901 gatcacttat ggactagaac gtcttgcttc atatattcaa gaatttgaaa gtgtctatga
    27961 tctggaatgg gctgatggtg tgaaatatgg cgagatcttc aaacaaccag aatatgaaca
    28021 ttccaaatat tcttttgaag taagcgacca agacttgcta ctaagcaatt ttgaccgttt
    28081 tgaaaaagaa gctaaacgct gtatcgatga aaatcttgtt catccagcgt atgattatat
    28141 tttgaaatgc agccatacat tcaacttatt agatgctcgc ggtgcagtat ccgtaacaga
    28201 acgagcaggt tatctttccc gtatccgcaa catggctcgt gctgtagcca aaatctttgt
    28261 ggcagaacgc gaaaaattag gattcccttt attgaataaa aacacagcaa atccagaaaa
    28321 catgaaggag gctgaataag atggcaaaag acttattact tgaaatcggg ctggaagaaa
    28381 tgcctgctca tgttgtcaca ccaagcatga aacaattaga agaaaaaaca gcaaaatttt
    28441 tagatgaaaa ccaattgact tatgatacga tcgaaacgtt ttctacaccg cgccgcttag
    28501 caatcaaagt aacaaacatt ccagaacgtc aagcagacag tgaagaagaa gtcaaaggac
    28561 cagccaaaaa aattgctttg gacgcagatg gaaactggac gaaagcagct gaaggatttg
    28621 tccgcggaca aggtctgacg acagatgcta tcacttttag agaattaaaa ggcgtggaat
    28681 atgtctatgt aacgaaatac actcacggca aagaagcaaa agaagtattg acaggtttag
    28741 cagatgtcat taaaagcctc actttcccag taacgatgca ctgggcagaa tacgattttg
    28801 aatatattcg ccctattcac tggatcgtgg cattgttaga tgacgaaata atcccgtttg
    28861 agatcttgga tgtcaaaaca gacagagctt caagaggtca tcgtttctta ggcgaagatg
    28921 taacatttgc gcatgcaaaa gaatacgaag aaaaattaaa tgaacaattt gttattgctt
    28981 caccgcaaaa aagaaaagaa atgatcatca cacaagcaaa cgaattagca gaaaaaaatc
    29041 actggatcat tgatctagat gaagatttac tagaagaagt caacaatcta gtcgaatatc
    29101 caactgtctt ttctggcgac ttcgaggaaa aatatctttc tgttcctgaa gaagtactag
    29161 tcacttctat gaaagaacat caacgttatt tcgaagtacg gaacgatcaa ggtatgttgc
    29221 ttcctcattt tatttctgct cgtaatggcg atcgggtaaa attggaaaat gtggtacgag
    29281 gcaatcaaaa agtattgact gctcgtttag aagatgcaga attcttttat aatgaagaca
    29341 agaaagcttc aatcgaccaa tacgttgaaa aattaaaaac tgttactttc catgaaaaaa
    29401 tcggtagcat ctacgagaaa atgcaacggg tacaagtcat cgcccgcatt ataggcgaac
    29461 aagtaggact atctgaggaa gaattgatgg atctgcaacg cgctgctgaa atctataaat
    29521 ttgatctagt aaccaacatg gtaggagaat tccctgaact acaagggatc atgggagaaa
    29581 aatatgctct gcttaaaggc gagaaaccag ccgtagcaac agcgatccgt gaacattaca
    29641 tgcctatatc cagtgaagga gatttacctg agactacagt aggttctgtt ttagcgattg
    29701 ctgataaatt agatagtatc ttttctttct ttgcagttgg gatgatccca agtggctcaa
    29761 acgatccata tgctttgcgc cgtcaagcat atggtgtcgt tcgaatcatc gaatcgaaaa
    29821 aatgggcctt tcctttgtct gttctgcaag aaacgattag tgaagttatc agtaaagata
    29881 cggatcgttt tggcattggc ttgtcagcgg gacaacaaca ggtgatcgac tttatcaaag
    29941 gccgtctgcg ccaattattg acaactaaga atattcgtca tgatgtcatc gaagctgttt
    30001 tgaatgcaga acaaaaagat ctgacaaaag tatttgcagc agcacaattg ttcaagcagc
    30061 atcttgcaga tgaagacttc aaaccatcga tggaagcttt gacacgtgtc gtgaacttag
    30121 cgaaaaaagc ggagcttgaa aaacaatcag aagtagaccc tgaactgttc gaaaacgaag
    30181 cggagaaaga actgcacaaa gcagtagagg caatgaaaac aagctttgct gaccaaacga
    30241 tcaacgaaaa ttatgatcag cttgtaagcc ttcgcccact gatcgaaaat tattttgatc
    30301 agacgatggt catggcagaa gaaccagcag tacgtaagaa ccgattaacg caattaagtc
    30361 aaattgctgc catggctttg tcactagcaa gtttagatca aatcatcaca aaataatttc
    30421 taatatgtaa ataaaccaga gaacaggcac aaaataggct atttctctgg tttatttgtt
    30481 ataaatataa ataactatta ttaattcaat gttaattttt ttgttatttt attttttgtg
    30541 taacttttaa aaacacctat gaaaattaag gaaataaaaa tgaaatcgat taattgttat
    30601 cttattaatg ttttatttac ttttaaatcg taaattgccc tagtcaagaa aacagtttgt
    30661 ggtatgatta tatagggaaa acttgtttac aaatatttct gtgaatatat tagtttaatt
    30721 atgttttttt aaataaaaag ctgtcatttt tatgtaagtg aattatatct taatttatct
    30781 atcatcaata cttaggagtg aatctatgga aaagccattc gtcaagtcat tgactgctgt
    30841 ggcggtaggt gcaggtgcta ttgcgatctg tttgtttgga taccatatca ataatcagcg
    30901 gcaacatcag caacggatca attatgcaga atctgccatc accaatcaaa aagacacctt
    30961 gacttcttta tcaaaagaag tagacaagct gtatagtaca aaagaaaaaa tttttctaaa
    31021 ccctgaaata acagaagaaa caatcacaag tttgtcaaat aagctatcct ctgtcaaact
    31081 ttcagcagat gattacgaca tcaaagaatc agaattgcct aaagaagctg ttgcaatcca
    31141 agatgagaag aaagcaatcg tgacacagtt agatagtgca gaatcaaaac tgaagatcca
    31201 gacagctgtc aacgatttat ttactaaaaa tgtatcaaac tggcagcaag ctgtagacga
    31261 tgtcatcatc aaagaaaagg tgacaagtgc agatgtcgct cgtgtacgtg aaaatatgag
    31321 cttttttaaa gatagtcctt ggaaaacggt tgttatgcaa tatctaggat tcgctgatac
    31381 ccagatcgcg caagtcacgc aactagatca gttattcgat acgatgttaa aggatggtca
    31441 agtgactgcc accgctactt acgatcaata tttgacggcg ctcagtcaaa tagagcagat
    31501 ccggaatgaa aaaataagcg tagcatacgc aacaaaagca caaactgttg ctcagcaaat
    31561 gggctatagc aatataaact attgacagtt aaagcgatga ttcaagcaag gttagcaaac
    31621 ggaggaaact atggcaagac gaagaagaaa atcaagaggg aaaaaaatat ttctgattat
    31681 tttagcagtt cttatcgtat tagtattagc aattgtagga gtaggagcaa aattatatct
    31741 ggatctctca cattctattg agaaaactta cgaatcagtg gaacgaaccc aaggagaacg
    31801 aacgaaacca gttgatctgg acaaacaaga atctttctct gttctattga tggggattga
    31861 tactggagat ttaggtcggg tcgatcaagg aagatcagat acgatgatgg ttgccacagt
    31921 cagtcctgaa gacaaacaga caacgatcgt cagcattcct cgagatacgt atgtcgacat
    31981 cgtaggtcgt gacacaaaag acaagatcaa ccacgcttac gcattcggcg gagctgccat
    32041 ggcgatggat accgtacaaa aatatgtaga catcccaatc gatcactatg tctctatcaa
    32101 tatgaaaggg ctaaaagaac ttgttgatgc agttggtggt atcgaagtca ataatgacct
    32161 cactttctca caagatggct atgactttac aatcggtaaa atcacattga acggggatca
    32221 ggcattatct tattctcgta tgcgttatga agatccaaat ggtgactatg gtagacaaga
    32281 gcgtcaaaga aagatcgtag aaggtgtagc gaaaaaagta ttgagtctaa acggtgtcag
    32341 caactatcaa tccgttttga atgctctaga atcgaatatg aaaacagaca tgagctttga
    32401 tgaaatgaaa aaaatcgctt tagattatcg ggaggcattt ggtacgatca agcaagatca
    32461 gctgcaagga gaaggcttca tgcaagataa tatctcctat caaaaagtca gcgatcaaga
    32521 acttgatcgt gtgaaaaaag aattaaaaga tcaaatgaat ctagagaata aataggaaag
    32581 tggagaaaaa atggaagaga caatcagctt gaaagaaatt tttggaacat tacgcaaaca
    32641 cttggcaaca atcatcatct caatgtttgc tggcttggcc atctcaggaa tcgtaacatt
    32701 tttcatcttg acacccaaat acagttcaca agcacaattg atcgtgacac tgccgcaatc
    32761 agacacagcg aatgtcaatg atgtcaatac gaacctgcaa atgatcaata cgtataaaga
    32821 tatgatcgtt agtgatctag tattaaatga agtcaaagat cgtctagaaa cagaagacaa
    32881 tgtgaaaatg accgctggac aaatcaaaga tgcgatctcc atcaatcagt cagaaaactc
    32941 acaaatgttc tcgatccagg caataagcac gaatgcagtg actgctcaac agatcgctaa
    33001 tacaacttca caagttttcc aagagaacgc aaaagacgtg atgaatgtcg ataaaatctc
    33061 gatcatctca ggtgcagttg ccaatacgac acctgtttcg ccaaataata aattgaatct
    33121 agtgattggc ttagtattag gaatgatggt aggcgtaggt ttggcattct tattcgaatt
    33181 acttgatcgg acagtgaaag atgaaaaata tgtgacggat acattaggat tcccaatctt
    33241 aggaacagtt ccagaaatgt cagcgaaaga attgaatgct ggtatccgta aaaaaggatc
    33301 agctcctgca cgaccaaaag ctacaaacga taaaacagcc ccatcacgca gaagccgctc
    33361 aagagtctag gaggaagaaa aatggcacga acacagaaac aaaaagcagc acaaactcat
    33421 gcagtcagtt tgatcacatt aacaaaccca tcttcaccaa tcgccgaaca atatcgtacg
    33481 atccggacga atatccagtt tgcctccgca gctggtcagc aaatcaaaac gattgtcgtg
    33541 acttcatcag gtcctggaga aggaaaatcc accactgcag caaacattgc agtcgttttt
    33601 gctaaatccg gtcaacgagt tctactagtc gatgcagatt tacgtaaacc cgtcgtctat
    33661 aagacattcc agctgaacaa tgccagtgga ttaagtacag cacttagttc atcaggaaat
    33721 gtagcagatg agatccaacg aacaccagtc gagaatttgt cgattctgcc aagtggacct
    33781 aaaccaccaa atccatcaga gttattaagc tcgccaagaa tggatcagat tttagcagaa
    33841 gcaagacaac tatttgatgt agtgatcttc gacatgccac cagtagtagc cgtaacagat
    33901 gcacagatca tgtcttcaaa aacggatggc actttattgg tcgtacgtga aaatacatca
    33961 cgtaaagaat ccttgactaa agcaaaagaa ctgctagaga tggtacaagc acgagtctta
    34021 ggtgttgtat acaacggagc agaacatagc aaagatgctg gttattacta tacagaaatt
    34081 aaggaataag aagagaggag acaagagaaa tgatcgatct acattgccat atcctaccag
    34141 gaatcgatga cggggcagaa aacttagaag caagtattgc aatggctgaa aaagcgattc
    34201 agcaaggcat cacgcatatt ttatgcacac cacaccacaa taatggaaaa tacagcaatg
    34261 aaaaatcaca agtcatttct ctagtcgctt cgttacaagc cgaattagag aaaagacagt
    34321 tgcctttgac gttattggaa ggacaagaag tacgtattac cggaacgctc atcgaagaca
    34381 tccaccgaga tgaaatttta tttacagatt tagatgatac atatcttctg atcgaatttc
    34441 cgacactgga agtgccgtta tatgcagaat ggcttttttt tgagttgtta gaactaggaa
    34501 agacaccagt gatcgtccat ccagaacgca acgcgcattt tcgagaagac ccaaatcatt
    34561 tgcttccgtt cctagatatg ggatgtttgg cccaaattac tgcgccaagt tatgttggtg
    34621 tatttggcaa agacatccag aaaacagcca aaacaatggt caaacataat ctcgtgcaga
    34681 tggctgcttc tgatgcacac ggggtctcta agcgcacatt ttacttaaaa gagtgctatg
    34741 agcagattgc caaggacttt ggtaaggaaa aagtagtaca gatgaaacaa gtcgcaaagg
    34801 atctgatcaa cggggatgag atcagttatc caatctatga agaggtaaaa aagaagaagt
    34861 ttggtttatt ttagttcttt attcgtagat aaaaacaaat gaggagagag tgaagatagt
    34921 gaaagggact gaaagtatgg aaagtgaaga tttattatct cgtattagtg aagtacatcc
    34981 taatcaagtt tggttggata aagaaaaagt tgaaaaacga tatatctatt tattttttaa
    35041 aagaatgatt gatattgtag gaagcattat tggtttgatt ttagcttctc ctttgatgtt
    35101 ctatgtagct tatcgaatca gaaaagaaga gccaggaagt ccgatatttt ttagccatac
    35161 acgtgttggt aaaaatggaa aaacattcaa aatgcataaa ttccgttcga tgtgtttaga
    35221 tgctgaagaa aaattggaag agttacttga tcaaaatgaa atagaaggcg caatgttcaa
    35281 gatgaaagaa gatccccgtg tcacccaaat aggtaaaatt atccgcgcta aaagcattga
    35341 tgaattgcca cagctatgga atgttttaaa aggagatatg agtctaattg gcccacgtcc
    35401 acctttggaa agggaagtag ctgagtacac gaaatatgat cttcaacgct tatgtgtaaa
    35461 acctggttgt tcaggtttgt ggcaggttag tgaaagaaat gatgttagtt tccataaaat
    35521 ggttgagttg gatttagagt atattggtca tcgaagttta atgtacgact tgaggttgat
    35581 tataaaaacg ttttatatta ttataaagcc taatggagca tattagttag ggatagaatg
    35641 atagattgtt gataaaaaag attacacatg ttcatattat cgtttcaaaa gtaatagctg
    35701 taattatgaa ggttttgaat cacttgtaga aaatctaaaa aaagaaaggt gaatggagca
    35761 attcagtatc attattctta tataaaagaa aatattctaa attttgggat gataccactc
    35821 ttaaatataa tcctctaaat gctttaatat tggtataccc gatataggaa ctgacgagct
    35881 atttagtatg atacaaaagt atatttatag ccattaataa attttccaaa gcttcctgga
    35941 accgtatttc ctcaaaacgt aatgtataca aattctattg aaaatgtctg caaatcgaaa
    36001 gaaattatat tgttttctgt accatcagtt tttgtaagaa atacagcaca aaaggtaaaa
    36061 ccgtatattt cagatgacca gataattgta gatgttgcca aaggaataga gtcagataca
    36121 ttaatgactc ttactgagat tattgaagat gagctgtcgg agcatagaat acatctagta
    36181 gtactttcag gcccgactca cgctgaagag gttgcaatgc aacttccaac aacaatagtt
    36241 tctgcgagta agaatcaaga tattgctaaa cgcgtatagg gaattttcac aagtccgtat
    36301 atgcgagtgt ataccaataa taatgtgaga ggcattgaaa tttgtggtgc attaaaaaat
    36361 ataattgcac tagccgccgg tatctctaaa ggaatgggat atggtgaata atgcaaaggc
    36421 cgcattgata accagaggac tagcagaact agagcgttta gtaggaagaa ttggttgttc
    36481 gcctaagacc tttagtggac agttaattgg tcagggtgtt ccaacagaac tggcgataaa
    36541 gcaagttgga atggtagtag aaggaataaa tgctctacca gcagctgtga aattatccaa
    36601 acagtttcaa gttgagttat ggcagttgat gcaattatca atggcagaaa gaagccaaaa
    36661 gaattagtag atgggctgat taatcgtgag caaaaatcgg aaaataccgt tccaaatata
    36721 gaacagtatt tttgaaactt cagtaattaa aagatgagga gtaaaacaat gaaaagagtt
    36781 attacatacg gaacatttga ccttttgcat tatggacata ttaatatcct tcgcagagca
    36841 aaggagcttg gagattattt agtagtcgtt ctttcctcgg atgagtttaa ctggaacgag
    36901 aagcagaaga aatgttattt cacctatgaa caaagaaagc agcttttgga gtctattcga
    36961 tatgttgatc tcgtaattcc tgaaacttgc tgggaacaga aactttctga tgtgcatgag
    37021 tatcacattg atacctttgt aatgggtgat gattgggagg gtaagtttga tttcttaaga
    37081 gaagaaggcg tggaggtggt ttatctgcct cgtactcctg aaatttctac gacccagatt
    37141 aagaaggatt tgaataaagt gtaagatata aagtctgcag ctgttaagtt atagaaatat
    37201 tgttaactat aggaaaaatg aattgcggag tgtgtctaga gctatttttg taagaggaga
    37261 aataatgaat acaaagaatc aaaaaatgaa agtgtgccta gttggatcat caggtggtca
    37321 tctagcacat ttgtatatgt tgaaaccatt ttgggagaat acggacagaa tttgggtcac
    37381 atttgataaa gttgatgcga aggcgatact gaaagatgag cgaatgatac cttgctattt
    37441 cccaactaat agaagcctta aagccttgat aattaactcg ataatagcgt ggagaacact
    37501 tagaaaagag aaacctgatt tgattatttc ttcgggtgca gcagttgccg ttccgttctt
    37561 ctatttggga aaactatttg gtgctaaaac aatttatatt gaagtattcg acagaattga
    37621 taaaccaaca gtcacgggta agctggttta ccctgtaaca gatagattta tagttgaatg
    37681 ggaagaaatg aaaaaggttt atccaaaagc tataaattta ggaagtctgt tttgaaagag
    37741 gagaattatt attaaggagg agttgtaaga tggtctttgt tactgtaggt actcatgaac
    37801 aacagtttaa cagattattg caggctgttg atagttgggc ggaaaataga gaagaaacgg
    37861 tagttgttca aaccgggtat agtacataca atctcgtcca ttgcatatca gaaaaatttt
    37921 tcccttataa tgaaatgatg gactatgtcc agaaggcgag aatagtaata tgtcatggtg
    37981 gaccagcaag ttttattatg ccactacagt atggtaaggt tccggttgtt gttccacgtc
    38041 aagtaaaata tgaagagcat gtagataatc atcaactaga tttttgtaaa gaagtcgaac
    38101 atagaatggg taatatcatt gttgttgaag aaatttcaga tcttactgat attttagata
    38161 aatattcggg aataattaat gataaagata aacaactctc tagcaataat gaactgtttt
    38221 gcaagaattt ttctatagtt gtcgatgagc tactaaaaaa tagagggagc caaatttgat
    38281 atgttcaaat atgacttgtc actgattatt ccagtatata atgctgaaag atatattgaa
    38341 gatacaatat ccgcagtagt taatcagact atttttccta atgtagaagt tataattgtg
    38401 aatgatggat ctagcgatga tacagaatca atttgtgaaa aatatgtaaa agattatgaa
    38461 aatattaaat taataaccca aaataatatg ggggtttctg aggccaggaa ttccggatta
    38521 cgtgtagcaa cggggaatta tgtaaccttt cttgatgcgg atgattttgt tgaggcagat
    38581 ttatatgaaa aagagcttca acttataaaa gataattcag cagacattgg agtagtagat
    38641 ttcaagaaag ttcatgatga tggaacaatt gttaaataca gaaaaaatta tgtgagaact
    38701 tggaatggcg ataattcctc tattattaag gactttttgt ctgggataat cggcaaccaa
    38761 gttgttgata aaattttctc gaaagctatt attaacgggc tgtctttttc tgggaaatat
    38821 aaaataggag aagacatgtt gttcatgtat caagccttag aacgtgctga aaaagttgta
    38881 atggatacat ctattgcgga ttaccattat attgttagaa gcgattctgc catgacggga
    38941 aaatttgatg aaaaatactt tgatccagtt attatttcag aaattattct cgatagctgt
    39001 gcatatgaaa aagaattgaa acattatgca caggctcact tgattcacga aatatgtaag
    39061 agttgtgagt acatttatag acataaggct gaaaagatgt gcccagataa gttgaagtat
    39121 ttgcgcaaca agctagctga atatagtgtt ttagatgcaa aaaaatattt gataaagaaa
    39181 caatttttgg gttttttatt aatgcgattt gtacccaaaa tctatatgct tgcacatagg
    39241 atgatgcaca taggatgagg aggatttgct tgcaagataa aaatttatca atcatcgttc
    39301 caatttataa tgttgcggac tatttggagc gttgtatgaa ttcaataata gctataaaaa
    39361 actttaaata tattacagta attttagttg atgatggctc tactgattca tcgggtctga
    39421 tcgcagatga gtatgcaaaa agattcgata atgtttgtgt ttatcataaa gtgaatggcg
    39481 gattatcaga tgcaagaaat tatggtttag tacgagcaga tacagaatat gtctttttta
    39541 tagattcaga tgacatgtta gttccggaga cttttgactt ggttataagt gaactagaac
    39601 agagttcatg tcaagtttta ttatttgatg ctaaagtagt agatgagaaa gatggttact
    39661 tagaaacggt ggatggcaat tattatgtac atggtggttt gagatcaaat tgttgttata
    39721 caggaaaaga tgtaattgaa ttacagctga cagatcataa tgattttgta acaacggtat
    39781 ggcttgggct ttataatcga gattttctaa tacaaaacaa tctatggttt gaaaaagggt
    39841 tgttacatga ggatgaatta tggacgatta agactctatt gaatgctcgg gagatccgat
    39901 atttaatgga gagtgcatac ctgtatagac agagagccgg atctataatg agttctaata
    39961 atcgtaattg gaataagaat atccaagctg taatatttat ttatcaaagt ttgtatgcat
    40021 atgtggattg gaaggtatct gatattgaat ttgcaagaat gcttaagggc aatttaacac
    40081 gaagatatgt tcatgcttta agtaaatttg aagtatataa atacagggaa atatataaaa
    40141 aaatacagtt tcgtgtgctt tatatgaact gtgttcgacc aattgataag attagagttt
    40201 taattttgaa atttacgccg aatatgtttc agtggattac taaactaatt ggtaagtcga
    40261 gatagtttta acatataact tagcttgaca gatagaaata ataaaagctc atatctataa
    40321 tttctagtca acatcttaga atttattata tgttataagt ggtgagatga tacgattagg
    40381 ttaatcccgt atttttacag gaatagtcgt gttacaacaa aattaatttt acgaaaggga
    40441 aatcgagaag atagttagac taattcatcg atcatagaaa aatggagatt ttatatgctt
    40501 tatttacagc tattttttgt acaggaagat tttggggaat agatgttgaa tggtacataa
    40561 gtgtaggggt aagaatactt attataggat tatttctggt atttcataaa tttagattta
    40621 agcgcaatta tattattgaa aagaatctta agcttgcgac tgtacctata attgctattg
    40681 tagtgtattc catagcggtg tggttgatgt ctggaagtgt tccagatgaa gatgtcgtta
    40741 gaaatctttt taccgctaac acatatttgg taattagtta tctgtttgca tctgtcctct
    40801 attatgaatt tggaaaaaat agcatagtat ttctaatgaa atgtggtgtt gtatcatatt
    40861 tactgggctc aattttgccc ctgataatca agatgccgag ggctgcgata ccatacctat
    40921 ttacttcgca cacaaatgat acatctttgt tgtggcttac ggaagttaac gatttaacat
    40981 tcgcttttgg tattatactt ttatatttct tgttttctta tcatcaaaac cataagaaaa
    41041 agaatattat aatatgtgcc ttgatgattt tttggggatt aaaacgtatt gaaattgcgg
    41101 caattctatt gtgttatctc ttttttaaag tcatcacgga aaaaatagta ttttcgcaag
    41161 gcgcgaaaat atttttagtg agtgttttat ctgtttcgta tctatatgta atgtttatcc
    41221 ataatgggca attagcaatg cttgcggaga tgtacaatat taactttatg ggaagattgc
    41281 taacttataa ctatgttaca gaaacatatt cgagcttttc accaggtttt ttaggcattg
    41341 gatttggccg tatcgatgaa attcttgatg atttagtagc tgaaaacttt agaattaatt
    41401 atgtccctgt tatatctttg catagtgatg tgcttagaat gtacataggc cttggattta
    41461 ttgcgtttgg tatatggatt ttttatcaat gctactatcg tacaatgaaa ttaaaaaaat
    41521 attttggctc caagactgct cagatatata tatgcttgac tctttatata tttattcttt
    41581 atttaactga taacacttat agttatccga ttactggtgc cacatatttt ttggctatat
    41641 tatgtgtaag cgatgaaaat cactttaacg cttatttgaa taaggagagg gctaaatgac
    41701 agaaaagtta tctagtgtgt gtttgattag tgtatatttt ggtaagtttc cagattggat
    41761 aaatgtgtgg tttctgtcat taaaacacaa tcctactata gattttattc tggttaccga
    41821 tcagaatgtt gatacctctg ggtatgataa tttaaaagta atcaatactg atttggattc
    41881 tttcaagatg ctattgcagt ctaagcttgg gatgaaaaat tcatcatata agctatgtga
    41941 ttacaaattg gtgtggggat tggctttaga gaatgagatt tccaagtatg actactgggg
    42001 acacagtgat atggatgttg tttatggtga catcagatct tttttgataa gatataaatt
    42061 gtgtgactat gataagttcg gtaatagagg acatttgaca ctgtatagaa acacgaaaga
    42121 ggttgctgag agatatcgtt taactggcag ttggttcgga tcatataagg aaatatttaa
    42181 atctaatagg atctgggcat ttgatgaaac cccaggcatt aatgctattt ataacgctca
    42241 caatttcagt catatgaatt cctttatata cgctgatata gatgtaaatt ataaagattt
    42301 gagattagta gatcgaaatg atccgcaata tgtaaattat caaaatcagg tattctattg
    42361 gaagaaagga aaattattac gtgcttatag taaagatgga acaatggttg tagatgaatt
    42421 tatgtatatt catttacaaa aaaaaagaat gcagaaacct gattttaact tagaagagtt
    42481 tgatggattt tatattttaa atggttactt tgaaccacta gggtcctgta tcaccttaga
    42541 cgatgttttg aaaaggaata ttcgaaatga tatagattta cctcaaaaaa tgttcggggg
    42601 ggggtacaaa aaagatttgc gattttattg gttaggacgc agatatttaa atatgaagta
    42661 cgacttgaaa caagttatca aaaagttgtt aaaacgttag gatagaggag cattatatca
    42721 ggtgtacatt ttggcgtttg tggatcatga ttgagaagaa aggttgaaac attctaattt
    42781 aagcaacatc cctaaaaaga gcgcataaaa ccaagcctcc taaaagcggg ctaaaaaatt
    42841 atgcctaaga cctaaattgt agatagagca gtaactaaat atccctgccg ttggatgatg
    42901 tagatagctt cttccaaaga aacctcacgg tgagttggag gtcggttact ctttcggtaa
    42961 agttccggat tataggggtc gtttttcttg aggatgttgt agatggtagt caacagtatt
    43021 ctggcaatag cgataatcgc ctttttgtgt ccccggtgct ttttgagtga aagatagcga
    43081 ttcctcaaag atattttaac aaattacaac atgctgcgtt aaggctatct gtgtctcatt
    43141 tcttttggtg ccttggagcg cagcggaaag gaatgctcat atctatgaaa ataaaaaaga
    43201 taaaagagta tataaaaaaa ggaacaatat tttcgctttt aagatataaa gtatcagtgt
    43261 ttttgcttaa aaaatgtaca cataataaaa atttggcggt tttgtctaga caatgcattg
    43321 ttggagaaaa actcaaaaag gaatttagtt atgttttaga aaataaatac aaggatcctt
    43381 tcgagggaaa atccaataag gtatggtttt gctggtttca agggtatgat gctgctccgg
    43441 aattaattaa ggcgtgttat cacgcggcaa aacaggcgat gccaaataaa gaaataatat
    43501 ttttaactgg tgataatatt aattcgtatg ttcatttacc tgattatata actgaaaaat
    43561 atcattcggg caagattggt tttgcacatt attctgattt aatacgtata tctttattat
    43621 gcgagtatgg cggcttatgg attgattcta cagtgttgac aacatcacca gagatggcag
    43681 aatacataac aagtttgccg ttctttgtat ttaaacaatt agagttgaca agattagatt
    43741 atcagccgac ggtagcttca aattggctga ttagttccta ttcgaataat aagattctct
    43801 tgcttacaag agatttttta tatgaatatt ggaaaaaata taattatgcg attgattatt
    43861 ttattttcca tatatttttc cgatttgcta cagaaaaata tagagaagat tgggaaaacg
    43921 ttcccacatt taataatatt tcgcctcaca tgcttcaaat ggagttgaac agtgagtata
    43981 atgaagaaag atggaaacaa cttcttctga taagcggatt tcataagctt aatcatcata
    44041 tagcatacaa cagagacaat actttttttg aatatgtcct taataggtat aagtcttttg
    44101 aggaggaatc atgaaaaata atcaagacaa ccttaaatat caagtaatgg gaggaatgtt
    44161 ttggaaatta tctgaaagga tattagcaca gggcgtttct tttctggttt cagtagtact
    44221 agctagaatg ttaacgccag atgagtacgg tatagtggca atcgtattgg ttctaatttc
    44281 gctagccaat gtgtttgtga caagtggttt tagtgttgcg ttagttcaaa ataagaattc
    44341 aaaagatata gatttttcta ccaatttttg gtgtagtata gctgtatcag tttgtattta
    44401 ttttttgctt tttataatcg cacctttaat agagttgttt tatgatataa aagggctgac
    44461 ctctgttatt agagtgtttg gattgataat tccattgagt gcatttagtg ccatacaaca
    44521 tgcatatgtt gaaagaaata tgctttttaa gaagtacttt ttttcgacat tatttggtac
    44581 agtagtttct ggaattacag gaattgtaat ggccatgtat ggaatgggag tatgggcttt
    44641 agtttttcaa tactttacta atacagcagt tgatataatt gtacttttgt ttacagtaaa
    44701 ttggagacca aagttcgaat tctctattct atccgcaaag catatgatga gctatggatg
    44761 gaagatattg gtggctgatt tctttggaac cttctttgaa cagcttcgat cacttattgt
    44821 tggaaaagtg tattcatcta gtgatttagc tttttataac aagggaaatc agttgccgac
    44881 attgataaca actaatgtta gttcttctat tatgacagtt ttatttcctg ccatagctaa
    44941 tattagcgat aattatgaaa gagtttatca aataacaaga cgtgcagttc aggtggttgc
    45001 atatatcatg tttcctttgc tttttggatt ggctgctgtt tcggataatc ttgtgttaat
    45061 attatttact gagaaatggc ttccagcttc tatttttgta aagattttaa gtataggtta
    45121 tgctataagt atgattggtt cagtgagtat tcagtcatta aaagctgttg gcagaagtga
    45181 tgtagtactt cgacttgaat ttattaaaaa acctatatac gtgttgctat taatattagg
    45241 tgtgaatata aacgtaacag ctgttgctgt tacgatggct atatataatt tgtatggagc
    45301 tttagttaat tccaatcaac ttagaaaagt aataggatac aaattcaaag aacagattca
    45361 agatttattt cctatgttac ttttatctac agtgatcttc gtagttgtta atatgctttc
    45421 aaatatagtc gaaaaccatt tatttacaat gataattcag attgcaattg gaactggact
    45481 atatattact atgtcagtat tgtttaagat agaagtattt ggttatttga aggaaatgat
    45541 agttttgagg tttaaaagta aatgaagaaa atattaaaaa tgataaaacg caaactacaa
    45601 atgttttgtg gaaaaaccaa aataattata gcgacagttt tatatggtaa gaataatatc
    45661 gaagcgcata agattgttgt tgataatttt ctgggcaaag gatacggtgg tgattgtaaa
    45721 tatataattg atgaattatt aaatcgaaag ttatcatgcg atattgtatg gcttgtaacg
    45781 gatgtgaatg cttctgtacc ggatggagta cgaaaggttc aatatggtac attacagagt
    45841 ttgaaggaat ttttaagtgc taaaatctgg atttctaatg ttaggaatac attaaagccc
    45901 actagaagaa cgggacaggt atatttgcag atctggcatg gtgcatatcc agtgaagtat
    45961 atagagggac agcaggaaga tctacttgat aaggattatg taaagcaagc caaaagagac
    46021 ggtcatgaca cgacggcgat tttagtaaat agcttgtata tggaagagat ttttaggaag
    46081 tatttctgga tgaataatca tgttgattat ttgcgttttg ggtatttgag aaatgattat
    46141 cttttaaaaa acatcgataa taaaagaaaa attgaagaaa ctcggaggaa attaggaata
    46201 aaaaatggag catatgtaat tctgtattgt ccaacattta gagataattt ttctagcaat
    46261 ggatatatac agaatgctaa actatttata gaaaatttgg aaagaaaata cggtagtgtc
    46321 gtcttattag tcagacttca tccaaatgac aaaaaaaata tttcattttt tgaatacaac
    46381 gataaagtaa taaatggctc aacggtctct gatggacttg atttagtatt agcttctgac
    46441 gctttgatta ctgattattc gtctatcgca tttgatggtg ctgtgttaaa taaaccagta
    46501 tatattttgg agaaagatat tgatacatac cgaactacta gaggattgtc agaacagtat
    46561 gatctttggc catttgttaa agtaaataat gaaatagatt tggttgaggc ctttatttca
    46621 tttgatagta acaaatataa aaaacgtctt aatgcatatt atcaatctga tatttcttat
    46681 gatactggag aaacggcaca gaaaacggta aattggatgt tgaagcagat gagtataatg
    46741 tgattagttg gttgaattga ggtgtgttag tagatggtca aatcagagga ttcaggagtt
    46801 tatgtattaa aaacaattgc ctgttttctt gtaattaatg tacattatac tataggggga
    46861 cagcttattc ctttctagat taacagtccc agtatttttt cttatttcag ggtgttattt
    46921 agtggaaaaa aacattacat ttcaatacat tagaaaaaaa ttgtttctat ttaaagtgct
    46981 tggtatatct gtagttttta cacaattctt aattactatg tatgtagttt taatggaaag
    47041 atggatgttt ggttaagcca aataaaagag ccatataatt ttttgaatct tatattattt
    47101 ggtaatgctt ctccatttat tggtgtggga catctatggt atttatttgc attactttac
    47161 gcttatatag gaatatatgc gctaaaaaga attactaata aagatagatt aaagattgca
    47221 atatttttat caatggtctt gtatctaata gaagtggtag attattatta tggctttact
    47281 gttcctgagt atctttatcg caatgttttt ttgaaggatg acccataatc atgataggta
    47341 tgaacattcc tgtctttcag agaaacttaa gctacaaaaa actgatagct attgtgatgg
    47401 cagcaggaat tatttcgatt gcagagtggt gtttatttca ggaacagtta gttgtgtata
    47461 taaattcata tatatttgct tgtgtgcttc ttattatagc tgtaaaaaaa tcacctaacg
    47521 tgaacaaaca taatttcttt gtgttttttg gaaagaactg cagtttaacg gcgttcatta
    47581 ttcactatgt attcattatt atactaagag gaatgaaaac accccggatt atcaagtatg
    47641 caatcgtttt agtattaagc acatttgtat ctttaatttt ttaccatata aagaaattaa
    47701 atgcaaatga gcagagaaat atatgataga gattgtatag tcactcaagt tgagtggtag
    47761 tcgtcctttt tgtcaaaatc ctccacgtgg cggatgatgg acaacgtttt gcattgacga
    47821 attgttgatt tagttttatt catacatccg caaggagggc gaagataaat ttgtggaata
    47881 gtaggtttta caggtgcaaa acaagctgcg ccagtattat tggatgaatt atccaagctt
    47941 gaatatagaa gggatgattc ggcaggtatc gctgttcgcg atggagaaaa ccttgtagaa
    48001 gtaataaagg cacgggtaag ctccgcaatc tgtctgataa aacagatgcg ggtagagcga
    48061 ttcgcggcac ttgtggtatt ggacatactc gttgggcagc acatggtgcg ccaagtcaga
    48121 caaatgcaca tcctcatgta tcggtaattg caccgggtct ggaccaggta ccgtggaatc
    48181 tgaggttgtt ggtgtatata acggtattat tgagaactac caggagttga aggaaaagct
    48241 cctaaaaaac ggatatacct tttatagtca gactgataca gaggtattga ttaagttagt
    48301 agattattat tataaaaaat ataaagtcgg tcctatcgat gccattgcaa aaacaatggt
    48361 ccgtgtgcgt ggcagttatg ccatggaggt aatgtttaag gattatccgg gagagattta
    48421 cgttgcccga aaagattctc caatgattat tggtattgct gatggtgaaa cctatatttt
    48481 ctctgatgtg cccgcgattc tgaagtatac caagcaggtt tactatattg acaataatga
    48541 aatggcccgt ctgtcagcag gtgaagcagt attttatgat ttaaatggtg atgtagttcc
    48601 aaaaaatctt acagagatta cctttaacgc agaagcggcg gaaaaaggtg gctttgagca
    48661 tttcatgatg aaggagattc atgagcagcc taaagccatc aaagatacaa tagcatctgt
    48721 ggtcactgaa aatggtatag atctttcgtc catgagcttt actgacgagg atattaacaa
    48781 tatttcacaa atttacattg tagcctgtag atctgcttgg catgttggta tagcggcaca
    48841 gtatgtatct gtggatttag caagggttcc tgttcgtgtt gagttagctt cggagtttag
    48901 atattgcaag cccttgctag ataaaaatgg actggttgtt gtgatctctc agtcaggtga
    48961 aatagcagac actcttgcag cactgcgaga agcaaaggct catgaggatt aagactcttg
    49021 ctattgttaa tgttgttgga agttccattg cgcgtgaagc agttaatgtg ttctatactt
    49081 tggcaggacc agagatttct gtagccacaa caaaggctta tagcgcgcag ttgatcgcaa
    49141 cttatctgat tgcaatgcaa tttgcaaagg ttcgtggcga tctgaaagat aattcatata
    49201 atacaatgct cgatgagatt caaaagctgc caggtaaggt agagcgtgtt attgaggaca
    49261 aagagcgtct ttagtggttt gcttctaaat atcctaatgc acataatgtt ttctttattg
    49321 gtcatggact agactacgct gtagctttag aaggaagttt aaagatgaag gaaattagct
    49381 atatccattc tgacgcctgt gcagcaggtg agttaaagca tggcactatt tccttgattg
    49441 aaaatggtac tttggttatt ggtctattga ctcagggtga tttatatgag aaaacaatca
    49501 gtaatatgat tgaatgcaac tcaagaggca catatttaat gggactcacc agttatggta
    49561 attacggggt ggaagatata gcagttttta cagtatatat tcctaagacc gatccccact
    49621 ttgctcctag ccttgaagtc atacctcttc aattgctagg gtattatctg agcatgtcca
    49681 aaggtttaga tgtagataaa ccccgtaaac ttgcaaagag tgttacggta gaataaataa
    49741 agaac
//
LOCUS       DACCKU010000009        48573 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.009, whole genome shotgun sequence.
ACCESSION   DACCKU010000009 DACCKU010000000
VERSION     DACCKU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 48573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.009"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(23..2095)
                     /locus_tag="IVN13_000480"
     CDS             complement(23..2095)
                     /locus_tag="IVN13_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7010998.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2028..2405)
                     /locus_tag="IVN13_000481"
     CDS             complement(2028..2405)
                     /locus_tag="IVN13_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP7010999.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(2534..3094)
                     /locus_tag="IVN13_000482"
     CDS             complement(2534..3094)
                     /locus_tag="IVN13_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7011000.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            3244..4536
                     /locus_tag="IVN13_000483"
     CDS             3244..4536
                     /locus_tag="IVN13_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7011001.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(4596..5714)
                     /locus_tag="IVN13_000484"
     CDS             complement(4596..5714)
                     /locus_tag="IVN13_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP7011002.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVN13_000485"
     CDS             complement(5728..7149)
                     /gene="arcD"
                     /locus_tag="IVN13_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP7011003.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVN13_000486"
     CDS             complement(7395..8516)
                     /gene="mnmA"
                     /locus_tag="IVN13_000486"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP7011004.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            8920..9519
                     /locus_tag="IVN13_000487"
     CDS             8920..9519
                     /locus_tag="IVN13_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP7011005.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVN13_000488"
     CDS             complement(9657..10943)
                     /gene="efmA"
                     /locus_tag="IVN13_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP7011006.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11214..11558)
                     /locus_tag="IVN13_000489"
     CDS             complement(11214..11558)
                     /locus_tag="IVN13_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP7011007.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(11695..12831)
                     /locus_tag="IVN13_000490"
     CDS             complement(11695..12831)
                     /locus_tag="IVN13_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7011008.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(13020..13994)
                     /locus_tag="IVN13_000491"
     CDS             complement(13020..13994)
                     /locus_tag="IVN13_000491"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7011009.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(14074..14949)
                     /locus_tag="IVN13_000492"
     CDS             complement(14074..14949)
                     /locus_tag="IVN13_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP7011010.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            15079..16986
                     /locus_tag="IVN13_000493"
     CDS             15079..16986
                     /locus_tag="IVN13_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP7011011.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(17527..20805)
                     /locus_tag="IVN13_000494"
     CDS             complement(17527..20805)
                     /locus_tag="IVN13_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP7011012.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(21076..21621)
                     /locus_tag="IVN13_000495"
     CDS             complement(21076..21621)
                     /locus_tag="IVN13_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7011013.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(21633..22325)
                     /locus_tag="IVN13_000496"
     CDS             complement(21633..22325)
                     /locus_tag="IVN13_000496"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP7011014.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(22344..22628)
                     /locus_tag="IVN13_000497"
     CDS             complement(22344..22628)
                     /locus_tag="IVN13_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011015.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(22641..23204)
                     /locus_tag="IVN13_000498"
     CDS             complement(22641..23204)
                     /locus_tag="IVN13_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7011016.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            23432..24094
                     /locus_tag="IVN13_000499"
     CDS             23432..24094
                     /locus_tag="IVN13_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP7011017.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            24218..25414
                     /locus_tag="IVN13_000500"
     CDS             24218..25414
                     /locus_tag="IVN13_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP7011018.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(25515..26891)
                     /locus_tag="IVN13_000501"
     CDS             complement(25515..26891)
                     /locus_tag="IVN13_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7011019.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(26928..27794)
                     /locus_tag="IVN13_000502"
     CDS             complement(26928..27794)
                     /locus_tag="IVN13_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP7011020.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(27883..28908)
                     /locus_tag="IVN13_000503"
     CDS             complement(27883..28908)
                     /locus_tag="IVN13_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7011021.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(28926..29600)
                     /locus_tag="IVN13_000504"
     CDS             complement(28926..29600)
                     /locus_tag="IVN13_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011022.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(29784..30677)
                     /locus_tag="IVN13_000505"
     CDS             complement(29784..30677)
                     /locus_tag="IVN13_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7011023.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(30751..32448)
                     /locus_tag="IVN13_000506"
     CDS             complement(30751..32448)
                     /locus_tag="IVN13_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7011024.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSKWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(32441..33754)
                     /locus_tag="IVN13_000507"
     CDS             complement(32441..33754)
                     /locus_tag="IVN13_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7011025.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(33754..37791)
                     /locus_tag="IVN13_000508"
     CDS             complement(33754..37791)
                     /locus_tag="IVN13_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7011026.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(37804..38262)
                     /locus_tag="IVN13_000509"
     CDS             complement(37804..38262)
                     /locus_tag="IVN13_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7011027.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(38560..39078)
                     /locus_tag="IVN13_000510"
     CDS             complement(38560..39078)
                     /locus_tag="IVN13_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7011028.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(39080..39697)
                     /locus_tag="IVN13_000511"
     CDS             complement(39080..39697)
                     /locus_tag="IVN13_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7011029.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(39694..40548)
                     /locus_tag="IVN13_000512"
     CDS             complement(39694..40548)
                     /locus_tag="IVN13_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7011030.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(40545..41456)
                     /locus_tag="IVN13_000513"
     CDS             complement(40545..41456)
                     /locus_tag="IVN13_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011031.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(41497..42831)
                     /locus_tag="IVN13_000514"
     CDS             complement(41497..42831)
                     /locus_tag="IVN13_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7011032.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            43039..43869
                     /locus_tag="IVN13_000515"
     CDS             43039..43869
                     /locus_tag="IVN13_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP7011033.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(44113..44362)
                     /locus_tag="IVN13_000516"
                     /pseudo
     CDS             complement(44113..44362)
                     /locus_tag="IVN13_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(44553..44747)
                     /locus_tag="IVN13_000517"
     CDS             complement(44553..44747)
                     /locus_tag="IVN13_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011034.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(45252..45437)
                     /locus_tag="IVN13_000518"
     CDS             complement(45252..45437)
                     /locus_tag="IVN13_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011035.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(46287..46628)
                     /locus_tag="IVN13_000519"
     CDS             complement(46287..46628)
                     /locus_tag="IVN13_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7011036.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(46628..46858)
                     /locus_tag="IVN13_000520"
     CDS             complement(46628..46858)
                     /locus_tag="IVN13_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7011037.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            47145..47483
                     /locus_tag="IVN13_000521"
     CDS             47145..47483
                     /locus_tag="IVN13_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011038.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(47688..47927)
                     /locus_tag="IVN13_000522"
     CDS             complement(47688..47927)
                     /locus_tag="IVN13_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP7011039.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(47903..48250)
                     /locus_tag="IVN13_000523"
     CDS             complement(47903..48250)
                     /locus_tag="IVN13_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP7011040.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        14111 a        10350 c         8511 g        15601 t
ORIGIN      
        1 tcacaacctc tttcatatct tattacttct tcattgctgc ttttagtgct gctgctaatg
       61 ggctttcttc tggttcttct gcttgtgcat attttttcag caagcgtcgt tcttcatgtt
      121 tggtcatttt cttcttctgc tcttttttat caagcaattt ttctgtcgtt ccatcgtatt
      181 tacaacggaa ataagcaccg tttttacctt cgatgatttc cattttccgg tgacattgcg
      241 ggcatcgatg attagagact tttggatctt ttcttctgcg gtagttgcaa tctgaattgg
      301 tgcagacata gattttgccg tcacgtgtgt ttttctcgcg cagattggcc ccacattctg
      361 ggcatttctt ttgggtaatc gagaaatctt gatattcttg tttgcttgat ttgatctcat
      421 tcaccaagcg ttttgtttct ttttcgattc cattcaagaa ggttcctgct tgtttctttc
      481 ctgcagcaat agcttcaagg tctttttccc agctttctgt caaatctggt gttactaaag
      541 agggatttac caatactaat aattgcttac ctttaggaga tacttgcagt ccttgagggg
      601 tacgttccat cagttcagat ttgattagtt tctcgataat ctctgcacgc gtagctgggg
      661 ttcccagact gtatttttcc atttgaccaa gcaagcttgc ttctgacaat ggtttaggcg
      721 gtgcagatag ttcttttttg atggcgaaat cgggattgat tctcattcct ttttcccatt
      781 tgactgtttc cgtctcagtt tcagtttcgc ctttccatcc tgcttggacg acacgatttt
      841 gccgaaaaat gaatcgttct ttaccaaatt caaccgtaac ttttgtttgg ctgactttat
      901 ggggttgagc aaacagtcct aaaaaacgag agacaatcat ttgatagatt tttcgctctt
      961 cattacttaa tttttcataa cgtgctctgt tttcagttgg caaaagtgca tggtgatcgg
     1021 tcactttact gtcttggaat acttttgtct gttggacgat ggctccgttt ttcaaatact
     1081 gcttgacctc aggagcaaag tcgctgactg cttgcaatcg ttctttcatc gtacttttca
     1141 tgtcatttgt cagatatttg ctgtctgtac gcggataagt aacgatctta tgggtctcat
     1201 acaaactttg tacaagactt aatgttttct ttgcagaata gccatagcgt tgattggctt
     1261 ctctttggat ctctgttaaa tcataaggca atggcgcaga ttccgttttt aatttttctt
     1321 ggacttctgt gacagttcct ttgcttgtac tgatcaattg aacaagtttt tgtgcttctt
     1381 cttgtgtttt taaagcaaat tgattttttt gaagcatttt ggcttgttct tttccagctt
     1441 gcaacgtgat cgtgaaatac gtttgtggac ggaactgctc gattttcttt tcttggtctc
     1501 tcactaatgc tagtgtcggt gtctgcacac ggcctgctga taaattatcc gcgtacttca
     1561 cagttaaggc tctggtcaca ttcaatccta ccagccagtc cgcttttgcc cgggcaagag
     1621 cagattcata caatgcatca tattctttcg caggtcttag ttgcttgaat ccagttttga
     1681 ttgctttatc tgtttgagaa gagatccata accgcttcac tggtttattg aaatgcacat
     1741 actctaagat ccagcgagca acaagttccc cttctcttcc ggcatctgtc gcaatgactg
     1801 cttctgagac atcttttcgc tgagccagct gcttgattgc ttttaattga tgtcctgttt
     1861 taggaagtgg tttgattcct agttttttag ggatcattgg caacgtgtcc atttgccagc
     1921 tctgccattc cttattcaga tcttccggca ttttcaatcc taataaatga ccaagggccc
     1981 aagtaacgat caccttgggt ccttcataat aatttttgtg cttttgatta gcaccaagca
     2041 ccttgcttag gtctttcgca acgcttggtt tttccgctag aattagttgt ttcataaaat
     2101 tcctttctgc tttctactgt gatgggctga tgatttttca acaacgcaag tcccttgtat
     2161 tgctgtaaat catcgtcaca ctcttcacac cagcgctcat ctccgtcatt ccaaaaggcg
     2221 ctgagaaagt tactttttgc attgatatat tcataatttt gatggatttc gttccattgt
     2281 ttttcgtacg cttgtcgtgc ttcttcgaaa gactcgaatg tttcttcttc ttcaatatct
     2341 tcttcccagt cttcaaaaaa ccaccaaggc tcattgtccc cgaacatggt aattacttga
     2401 tacatactcg tgctccttct ttcagctttt ctagactcat cttagtcact tttcaagaaa
     2461 gttgcaagaa ttttctctta taaaagggtc atttaatgaa catcttaaaa ttgttacaaa
     2521 tgaatctgat tgtttaaggc aaagtagcga taaagttatt gatcatccaa aaatagctgt
     2581 cttctggatc gattgttggt tcttttgtaa agtatcctaa aaaacgtaag gaaagataac
     2641 cgtggatcat actacgaagt tccctgcttc gatgaacttt ttcttctttc gttaattgaa
     2701 aagcctcaaa tatctgcaaa atgatctgat tcgtttcatg gattccatct aataaaacag
     2761 cttcattggt atgcggtaca ctgatcagta actcatacac tgcttgattt gaaaaagcaa
     2821 aggatcgata agtttctgca tagatctgca gtgcttcctg accacttttt cccacaagag
     2881 cccgtcgcag ctggtcatta agttcctgca gaagtttcgc tgttagcgct gttttgacat
     2941 cacgaatatt tttgaaatgg ttatacagcg agggagcctt gatatccaag cgtttggcta
     3001 aatgctgaaa ggttatttgc tgaacatcca tctcttctgc tagttctcga aagcagtcga
     3061 tgattttctc ttgagacaga tttctttttt tcatttccat gtttcctctc ataaaataaa
     3121 tccttcactc tttcagttta ccacgaaaaa gaaagagaaa aaagagactt tcattgataa
     3181 actacatatt gtagtttata ataaagagct gtagatgaaa attttcagaa agaaggagtc
     3241 taattggctg caagaataaa ggaaaaatta tttaggaaaa gagaaacttg ggaacaaaac
     3301 ttgtttgtct tatggtttgg aacatttatg gctggtatcg gatttagttt agtcatgcct
     3361 tttatgtcac tttacataga tacgttaggt aattatagta cgtcacaatt gaacttttgg
     3421 agcggattga ctttttcatc tactttcttg gtgactacgc ttatctctcc ttggtgggga
     3481 aaattagccg accaaaaagg aagaaaattg atgctgttgc gtgcttcatt aggaatggca
     3541 atcgtcattt ctttgatggg ttgcgtgact agtgtgtatc aattagtcgc attgcgtctg
     3601 cttcaaggga ttttttctgg ttatatcagt aatgccactg cccttgtcgc aactgggacg
     3661 ccaaaagaaa aaagtggtca agttcttgga accttagcaa ctggttctgt tacaggaaca
     3721 ttacttggac cgcttcttgg cgggatctct gcttcagcgt ttggctatcg ccctaccttt
     3781 ttcattacag ggatcatctt atttttcgtc ttcttgttga gtctgttttt cgtccatgaa
     3841 aaatttgtcc cagtgaaaaa agaaaacatg gtttctgcca aacagatttt caaagacttg
     3901 aaatatccac acgtcgtcat cggtatgttc gtcacaacga tgatcatcca agcatccaac
     3961 aattccatta gtccaatcat cagtctgtac attcgccaat tgctgcatgg acatgggaat
     4021 gttacgttga tcagcggaat cattgcttcg atacctggga ttgccacttt gatagccgct
     4081 ccaagatttg gtcgcttagg tgacaagatt gggagtgaaa gaatacttgc gattggtttg
     4141 ggttttgcga ttctggttta tatcccaatg gcattcgtca cgaatgtttg gcaattagct
     4201 gccttacgtt ttttgattgg tatctctgat gcttgtctgc tccctgctgt ccaagcattg
     4261 atcacaaaat attcgcctca tcaagctgct ggacggatct tcagttataa ccaatctttc
     4321 caagcaactg gtaacgtagt agggcctatg atcggctcca gtgtttccag tgcatttggt
     4381 tatcgcggcg tctttctttc gacttctctt cttgttttgg tcaacttctt gcttgtacga
     4441 cacaatacac aagagatcca tcagcaagaa actgcagaag aaaaagcttc aggagtctcc
     4501 atagcttcag ggcactctcc ttctcataga caataagcgt aaatgaaaaa cactgcttga
     4561 acagttgtca cagactgtcc ttgcagtgtt tttgtttagg cctgtttttt caagaactca
     4621 gataagagga ataatccctt ttccaaggtt tcttttttac aacagtaacc tagtcgggca
     4681 tgcttcggta aatcaaaggc acttcctggt actaatagta ctcctgtttc ttctaataaa
     4741 ttgatacaaa atgtctggtc atcttcttcg atggctaatt taatgaaaga agtagagacg
     4801 taattgggag cgacaagttc aactttcggt tcattggcta cccattgttt taaaatcaca
     4861 aggttctctg taatgatctt tttgtttcgc gccaatattt tttctttatt cttcaatgca
     4921 tgtacagcaa gatcatctga caggactccg ccgcaaatca tcgtgtagtc tcgatacttt
     4981 cgaaaaactt ctgccaactc aggtccagta gctgtccagc caatacgaat acctggaatg
     5041 gagtaggttt tggataggga attggttgct ataccttttt catagacatc tacgatcgac
     5101 aagaattcac ctttatccgt taaaggcgca tatacttcat ctatcaatac atatgcatct
     5161 actgtacgag caatttctgc gattttttct aaagttttcc gatctagcaa tgtgcctgta
     5221 ggattgtttg cactattcaa acagatgatt tttgtttcag gtttgactaa ggcttctagc
     5281 tgttctaaat caaactgcca atcttcttct tccttcaaat gaacaaagtc aacagcggca
     5341 cctaaagatt tggggatgtc atataattgc tgataagtag gaagcatcga aacaacatgg
     5401 tctcctgctt ccactaacgc gtatagagct aaaagattag caccagtcgc tccattcgtt
     5461 tgtaagatgt tttccggatc gacggtttga taaagagaag cgacttcttg tttgaaaaca
     5521 tctgatcctt cgatccagcc atagtccaat ggttcgtttt tgcgtttttc gaaaaattta
     5581 cttacgctag tcccatctaa gccaacgagt tcgtctaatg tcaatgcttc gatcgagctt
     5641 tgtgcaatat catataccgc tttcttttcc catttattta gccattcttc tacaccgaaa
     5701 tttgcgattt tcatgtttct ttccccctta tatcttgatc cagcctacag caagcaaccc
     5761 gattgaaagc agtgcggcca cacaaatcag cccaatcatt actttttctt ttcccgtaat
     5821 ttgatgtttg gtttcacgac aagcgatcca gtagaagcaa aaaccaggaa gataggcaat
     5881 actacataaa aggacataag aaaatcctgc taaaatgatg cccatcaatt caaaaactac
     5941 agcaatccca ccgatcacta cttgtttttt gattccttct tttaaagaaa actggacttg
     6001 atagatcccg acaagtagat aacaaatcaa aattgccgct gtacataaag aataggcaaa
     6061 ttgataggca taatcagtaa ctaagaaagt caataagaat agattagtca agattgcagt
     6121 aacaactaac gaataagttg gggcattttt tttattcgtt tttccccaac ctttcggcaa
     6181 cagaccgtct cgagccatca acatcgttgt ttctgctggg agcatcgtcc aagacaacca
     6241 agaaccaatg atcgaaataa tcaagccgat attgatcagc gctgctcccc aatttcccac
     6301 gattcctttt aaaatatttg ccatagcagg ttgtgaaata gttgctaact cttcttgaga
     6361 taagacgccg tatggcaaga tcgaagcaag gatatagata aacaataaac aaagcagacc
     6421 aatgatcgat gcttgctggg cttctgaacg tttcttcgcg cggttggcta aaacacttgc
     6481 gccttcgatc cctacaaaga cccacatcat aaccatcaaa caacctttaa tctgcaacca
     6541 gatcgttcct tgctgccctg ttccattcac tagattatca gatacgttac cccaaaaatc
     6601 agcagtgaat atattcactt taaatgcgat aaatacaaat attaaaaata ggaataatgg
     6661 cactaattta cagatcgtca cgattgtatt gatgacgctc gctccttcaa ctccattgtt
     6721 tactaataaa gtcagacccc aaataaaaat acttgcaaat aaaatagaag gaacattttg
     6781 tccacctttg aatatcggga aaaattctcc catggcgctc atcagcatcg ttgcaaaagc
     6841 aacatttccc agccacgcag acaaccagta accccaacca ctgatgaagc cacctaattt
     6901 accaaatgaa gcttccgcgt aaccgaaaat accaccatct agatctggac gcttttctcc
     6961 taaatgattc agcgataata ccaaagctaa aataccaatc ccaacgatta tccatgaaag
     7021 gatcgctggc cctggagctg cagaactagc cagatcactt gtgatcccaa acacaccgct
     7081 tccgattgac gaactgacaa ccagagcaac cagtccccat ttgctaatac ccgtcgattt
     7141 cttttccatt tccatcaccc tcacttataa aattgaacaa atcacttgca aaagattccc
     7201 ttctattttg caactgctct tgcttaacaa aaagcaactt tattcatatt attggatatt
     7261 atgaactttt tacacaagta tttcaagtat aaaatgaata aaaattaatt ttattgcaaa
     7321 aaaacagcta tgctatccaa aagaaaaggt gtttcttttg ttagccatag ctgttttgat
     7381 tcatttaata tcgcttaaac gtattgtaaa acttttgttt cttgatatgc gtggtcgatc
     7441 aaaccaccac ccagacattc cattccatca taaaatacaa ctgcttgtcc aggagtgatt
     7501 gcacgtacag gttcgtcgaa tacgacttct gcacgattat catccaataa gcgaactgtc
     7561 acaggaacgt cttgctgacg ataacggaat ttcgctgtac acttgaattc ttttggcatt
     7621 ggttcattcg tcgtaaagtg gatttcactt gcatccaagc ttgtggcata caatgcagga
     7681 tgatggaacc cttgccccac atataatgtg tttgtcgcta gatcttttcc tacaacaaac
     7741 cagggttctt gcgaatctcc tccgccaccg atccctagtc cttgacgttg accaatcgta
     7801 taatacatca agccagcatg ttgtcctttg acttcaccat caagagtgac catatttcct
     7861 ttttttgctg gcagatagtt gctcaaaaat tgtttgaagt tcttttcacc aataaagcag
     7921 attcccgttg aatctttttt cttagcagtc gcaaggcctg cgcgttcagc aattgcccgt
     7981 acttctgatt tttccatgcc gcctagaggg aacatcgttt ttgaaagctg ttcttgtgat
     8041 aattggctaa ggaagtaagt ttgatcttta ttattgtcta ctccacgaag catgtggacc
     8101 acgccgtttt catcgcgagt gacttgcgca taatgtccag tagctacata atcggcgcca
     8161 agatccattg cataatccaa gaatgctttg aatttgattt ctttgttgca catcacgtct
     8221 gggttaggtg tacgtcctgc gcggtattcg gctaggaaat attcaaatac acgatcccaa
     8281 tattcttttt caaaattcac agagtaataa ggaatcccga tttgagcagc tactttcgct
     8341 acatccttat aatcttccgt ggccgtgcat acaccgtttt cgtccgtgtc atcccaattt
     8401 ttcatgaata ttccgatcac gtcgtatcct tgttccttta acaaaagtgc tgttacagat
     8461 gagtcgacac cgccgctcat ccctacgaca acgcgtgttt tgctgttgtc tgtcatgata
     8521 tcaccatcat ttcaattaga ttctgaaaca tctacgagtt gaaggtcgca acttttcagt
     8581 attttacatt atacgtcata tttctgaaaa tttcatccct taattatcaa gtcgtaaaaa
     8641 tactgttcta ctggacctat tcagtacaca aacatgatag tcaaccaaaa aatcactcat
     8701 cttttgagca ttcgatataa aaaattggca tactcataaa aagggccagc ctttcggcaa
     8761 taaggaataa ttttataaat tgtgaaaaac tatttttcaa aattgcttct tatcgcttaa
     8821 agctgcacgt ctcatttaca accttttctt tttagatgtt atgagttgct cagataagtg
     8881 attttgttta ctctaagaaa aaagggaggc aaaagataca tgaattactt tatcagtgat
     8941 atgcactttt tccatgaacg cttattagga aaaaacgact ttgctccgcg gctctttgca
     9001 aatgtggatg aaatgaacca acagttgatc actagctgga atgcagtaat aaaagaaacc
     9061 gaccatgttt atcatctagg cgaccttgct atgcatccgc aatatgaaaa agcaaatgca
     9121 gaaattttgg aactggtcac tcaactgcaa ggaacaatcc acttgatcaa agggaaccat
     9181 gacagtcgcg cttttttccg ttatttagaa aaaaatgatc ctaggcttac tgacggaagt
     9241 cccaaatttt atttttatga tgtaggtgcc atcgttaaat tcgaccatca acaatattac
     9301 ttgactcatt atccgatgtt gctcgggcct aacgacacta tccgcaatct tcatggacat
     9361 atccatcatt acagcgtacc tatcgagaac gatgtcaatg taggcgtaga cgcaccggaa
     9421 agagaactct taaaggaaaa actaccattt ggcactccgt tatctgagca acaaatcaaa
     9481 gaaatctatg aagcaaagag gaagtacgat aggtcgtgaa aaaactgccc tatatcaagt
     9541 attaaacaaa taaagataat tactcatatt tttcatttga gtaggcttat tgccacagga
     9601 acattaggat cagaaatgga gttgtaacaa gacttctgtc acaactccat tccatactat
     9661 tcgtcttttt gctcatggtg ttcttttacc agttttttct gtaattcttt atcataattt
     9721 cgggcagctg acgttaaaaa taaaacgatt ccgcataata aaatcccgat tccggaaaac
     9781 aagaacattt tttctacccc taaacgatca gctaatggtc ctgcaaataa taatccaatt
     9841 ggacctgcca ggttcatcaa agagttgaag ttccctaaga cacgacctaa aacattcgat
     9901 tcataactct gctggatcat tgccatcagc agtgtattga aatatggcgt tgcacaacct
     9961 gctcctgcat tcaagataag aaagtacaag aacccttgac tatcgtttgg aaccaatccg
    10021 cttgcaccga tcgtcaggcc cataatgatg taggctataa taataggttt cattcggtct
    10081 ttccatgttc ctataaaact gatcagtgca ccgcctaaga gcattccgac tgcgtagact
    10141 acttccactg ctcccgcctg tccaacggaa ccaccaaagt aatctagcgt catcaatgga
    10201 tacagactga ttgctggcat gaacaacagc atcacaaatg caccattgat cgttatgtac
    10261 cataaccctt tattatccat caattgctgc agcccaaatt ttgcatcttt aaaaatagta
    10321 agcggaactt ccaatatttc aggagaaact tttgggatct tcacaaaaat caataagccg
    10381 acaccaaaaa ctgcccccaa tacatctaat aaaataaggt aattgacagg aacgactgaa
    10441 aacaaagccg cacctagagc tggcgcaatg atgaaattcg ctgattggac cattcctaac
    10501 tgtccattcg tccttgttat gtgagaagat ggaacgattg tcggcataat cgactggatc
    10561 gttggcattt ggaatgtttg tgcgaccgaa cgcataaaca aagacacaaa aactagccaa
    10621 atcgggaagg attctgaaat cgttccgact actgctaaaa taagcgcgaa aatcgctaca
    10681 ataatatccg tgacaatcaa aagagctttt ttatcccagc gatccactag cggtcctaca
    10741 aatgggctta gtaaaaccat gggaatcatt cctagtaaag tagcaaaact caaaattgtc
    10801 gccgaaccgg tttctcttgt caaataccag atgatcgcat attggacgac catactagtg
    10861 attcctgaca aaaactgtcc cgataaaaag aacaaataat ttctcttcca attcgttaaa
    10921 acgaccgact gttcattttc cataaacatc cctcttttct ttttcatttt gtatagcgtt
    10981 tgcacaacaa tcattatacg ggattctttt ttcaaaaaat acctttttcc ctaaataaca
    11041 atcgtttcaa tttaaatttc tttacttaga aacaataaat cagcaaaata ttaaaaataa
    11101 caacatgtgt taaatttaaa aataaaaaag acaactaatt tgcgtcgtcc ttacaaagtt
    11161 agtcatctaa caataggccg tttcaaaact agttgtcttt ttatatccac agatcattcg
    11221 actttttcga ataacccacg gctaatcatc ccatccatca gaaaataaaa tttatcttgg
    11281 ctcattttct gttttggatc tttgaaaata ttgactgctt tcaatccatt tgctgtagtg
    11341 atcgacattt tcaatgtttg aatatctttt gatacagtga tttttaattt gaatccttct
    11401 ttgctttgcg gcgtggcatc taatggtcgg atcaattgat aattgcctcc gtttgtttct
    11461 gtaaatccat tatcgcgcaa tgtatatctt tttgcatcag gatgcaaacg atagctaatt
    11521 ggtgcgccca gtaccgttgc tgttgtttca aatgccatga ttttcacctc gataaaatta
    11581 gtttctcttt attataaaag tttatcctga tttttactag gtttttcagt taagttccct
    11641 aaaaaatgaa ggcgttctct cagacatctg ttcatataag aatgacttca ctagttattt
    11701 tttgacagca cgaactaatt gatcaaggaa atactggatg tcgtcttcgg tatttcccaa
    11761 accaaagctg atacggatgg attcttttag tatcggtgta tcttctccat acattgcttc
    11821 taatacgtgt gaaggctcga tatttcctgc tgtacaagct gaaccagtag aaatagcgac
    11881 cccattaaga tcaagcttca tcagtagcaa atcatttgct tcgccaatta agcgaagatt
    11941 caacacatga gggagtttat tcgttaaatc accatttact tgataaggga tttcgtttgc
    12001 ttctaatgta tcaagaatca attgctggaa tcctttatat ttgttgttac gtgcttcacg
    12061 ttcttcaggt gacagcagtt caactgcttt tgccataccc cagatccctg ctaaattttc
    12121 tgtacctgca cgccgctttt cttcctgctc cccgcctaaa agcaatgcag ggatatttac
    12181 tccgtctcgt ttatataaga aaccgacgcc ttttggccca ttgattttat gagcagagat
    12241 acttaataaa tcgattccta attcttctgg atggatcgtt tggtttccgt acgcttgtac
    12301 cgcatctgta tggaaataag ctggatgatc cgctaaaagt tcaccgattt ctttgattgg
    12361 catcaaattt ccgatttcat tgttcccgta catcacagag accaagatcg tatcttctcg
    12421 taacgctttt ttgacctctt cgatcgacag attccctttt tcatctacag gcagatatgt
    12481 aacgtcaaac ccctttgtct ctaaatattt catggtatta agaacagcag gatgttcgat
    12541 agctgtagtg attagatgtc tgccttcttt ttgacgcgag aacgctgttt ctaagatagc
    12601 tgtattatcc ccttctgttc ccccactgtt aaagatgatc tcgtgaggtt ttgcttgcag
    12661 actgtctgca atgatttgtc ttgctgtttc caatttttca tgcgcgtggc gaccaaatcc
    12721 atgtatgctt gaaggatttc caaacgtgtc ttgcatgatt gccgtcatcg cttcaattac
    12781 ttgtggatgc atgggggtag ttgccgcatg atccagataa atagttttca cttcattcat
    12841 cccttccaaa ttattgctag gcacattcta gcataaaata gcgaacaaac caaagaagag
    12901 ctcatttttg tctagaggac gtttcatggg catagaaaaa aggagctgtg acaaaagtct
    12961 tgtcgcagct ccttgaagct gctcacttct gtcacaactt ctttccctct tttttttgtt
    13021 tattcgaatt ctttttcttc cagacggaat aacggactca tcggtgtatt ttcatggatg
    13081 cgtttgaccg cttcacccat taattctgaa catgtaacga ttgttaagtt ttctggatga
    13141 cgattttctt ctgtgaaaac agaatcagtg atacaaatat ctttgattgg tgcattgtct
    13201 aaaatacttt tcgcatgatc ggacaataat ccatgtgacg cacaggcata tacttctttt
    13261 gcgccgtttt cttttaatac atttgctgct cgtgcaagcg ttgctcctgt gttcaagata
    13321 tcatctacca tgatacatgt ctttccttga acatcaccga tcacataacc accctcagtt
    13381 gaagcatcat ctgcatgatc tacgatggct aatgtgctgt tcaaatattc tgaaagacta
    13441 cgtgcacgct gaacaccgct gttttttggc gaaacaacta cgatgtcatc tcccgtcaga
    13501 cctaattgac ggtaataatg agcaaacaac ggcatcgtaa ataaattatc tacaggaata
    13561 tcaaagaatc cttgtacttg aaccgtgtgc agatctaaag tcagtactcg agttgcccct
    13621 gcttcttcga tcaagttagc gatcaatttc gcagtgatcg gttcatgagg tttcgccgtg
    13681 cggtcttgcc tagcataccc atagtaaggc aatacgacat tgatcgtttt cgcacttgct
    13741 cgcttcattg catcgatcat gatcagtagt tccatatagt aatcattgac aggtgcattc
    13801 gttgattgaa cgatatacac atgatcccct cgaatacttt cttcgatatt gatggaaatc
    13861 tcgccatcac taaattgctt cacgacgctt ttacctaatt ctgttccgaa cactttcgcg
    13921 attttttcag ctaatggacg gttgccattc aaactaaaaa tcttcaacgt gtcatcctga
    13981 tacttattac tcatgtagtc ctccccaaaa cttctctatt cttcgatagt ttaacatatt
    14041 tttccaagat tttctgttaa tttcagcaaa ttcttaagga agtgaaactg ttccgttact
    14101 ccaactggca ggaatgactt tatgataatc gccccagttg tcgtgtactt tgtaataacg
    14161 ttctcctgtt tcagtttcaa aataagcgta cgctgtcacc caatgattgc catatgtact
    14221 gccaagtact ttcaaaatac caatcatcac aggtttgcct tcacgtatac gttttgtagc
    14281 tcgctgccat gaacctagaa gagttgctcg tgcacgctca tgattgccat tcttctttaa
    14341 aaaactagaa atccctgtac taacctgaaa aggaatcgta ggaagtccta gtggttggat
    14401 ctggctacgc aatgccttcg tcaaaaccaa cgaatcagcc gtatttttct tgcgaatctc
    14461 atttggaagc atccacccct tacgatagtc ttgataatag gcaaggagaa ctgctgccgc
    14521 gtaagtacca caaagatagc ctgagggggt agcccatttc ttatacctca aaaaacgttc
    14581 ttctggcaat cccacccagc aatttgcgag ttcctttcct gacctttttt cttcgatcaa
    14641 aacgggaata tcttcttttc tgttgatcca tctagataac tcgattggct cttcattgat
    14701 cagcttccat tttttacaag aaagaccctc ttgataagta tattttgctg gagaatattc
    14761 taccggatgt cctgattgat cgataatcac ataactttga tcttcaaaaa ccagagctaa
    14821 aaaaagcaat ttactttttc ttgactgcag ctgctgagtg ctggctaatt ttttaatttt
    14881 tttatctact ttgaaatata aaggtttatc cactacttga ttcagttggg atcgcctcct
    14941 ttttttcatt ataagtgaag gattgaaaaa aacaaatcag tttgattgtt cgaattttca
    15001 attttttatg ttctatttgt taattccttc attttgtaag ataatgggca cacgaaacaa
    15061 aaaaggagcg atcgatgaat gaacaaagaa cgattgaaac aagatttcta tgaagcagtc
    15121 aatgaagaat ggcaaaaaac agcaaaaatc cctgcagata aaccagctac aggcggtttc
    15181 caagatttag ttgacgggat cgaccaactt ttgatgggag aaacagacga tctgcttgcc
    15241 catccagaaa aagtgtcttc agaaaaaatg aaacatttcc ttgcatatta tcaactggca
    15301 aacgattatg accgaagaaa tgagttgcat gctcaaccgc ttcaacctat aatcgaaaaa
    15361 gttgaatcat tgcagtcttt cgacgaattg aataagcaat ttccagaatg gctgttggat
    15421 acattaccat tgccatttaa tctggatgta gatgcagata tgaagaatgc gcaaacgaac
    15481 gccttctttg cttatccgcc ttctttattc cttccagata agacgtatta tgcatcagac
    15541 catcccaacg gtgcacaact aataggcgtt ttctttgata tggctgttca gttattagag
    15601 cttgccggca aagaaaaaag tcaggcagaa gcaatcacgg aacaagcagt gcaatttgat
    15661 aaattgattg ctcctcatgt aaaaagtgca gaagaaaacg cagattacag caaaatgtat
    15721 aatccgcaat cttttgatac atttattaaa tacacagata aactggactt gaaaaaactt
    15781 gtcacgggtt tgattgggac aactccagat aaagtgatcg taactgatcc agtgtacttc
    15841 gatcatttga atgacatcct gacagaagct cattttccgt tattcaaaag ctggatgatc
    15901 attaaaacag tacgttctct tagtggttat ctatcagaag aattccgcca agtcagcgga
    15961 atctattctc gcgtattgtc tggaacagac gaagcaatgc ctcagaaaaa agcagcgtat
    16021 taccttgcct ctggacaatt cgatcaagta gttggcgatt actacggaaa aaaatatttt
    16081 ggcgaagctg ctaaaaaaga cgtagaacaa atggtgaaaa aaatgatcgc cgtttataaa
    16141 aaacgtctag aagaaaatga ctggcttggg gatgcaacaa aagaaaaagc cgtcataaaa
    16201 ttaaataact taggtatcca agtaggctac cccgataaga tcccagcatt attcgaccag
    16261 ttcaaaacag tacctgcaga tcagggtggt actttgcttt caaatactct tgttttcagc
    16321 aaactcacat taaaagatcg tttcagcaaa tggaacaaac cagtggatcg ttcagaatgg
    16381 gaaatgagtg ctgacacggt caatgcttat tatcatccat ttcggaatgt gatcgtcttt
    16441 ccggcagcga tccttcaagc accattttac agtctagaac aatcaagctc tgctaattat
    16501 ggtggaatcg gagctgttat cgctcatgag atttcccatg catttgataa taacggcgct
    16561 ttatttgacg aatatggcaa cttgaacaac tggtggacag aagaagatct tacacaattt
    16621 caaaacaaag cacaagcaat gatcgaagaa ttcgatggtc tgccttttgc tgacggaaaa
    16681 gtaaacggaa aattaaccgt ttcggaaaat attgctgatg caggcggtct aagctgtgca
    16741 ttggaagcag caaaaacgga atcagatctt tctatcgagg aattctttgt caattgggca
    16801 acgatttggc gtacaaaagc aaaaaaagaa taccaacaac ttttactgca aatcgatgtc
    16861 catgcccctg ctaaactccg agcaaacgtg caaccaaaaa accttgacga attctatgaa
    16921 acatttggta tcacggaaca agacgctatg tatctagcgc cagaaaaaag agtgcgaatc
    16981 tggtaagagg ttgtgccaga agtggtcagc ttcaagaaat aagaaggaat ttgggaaaat
    17041 cgcttctcaa atttttgtga catttcggct tatttttcga agaagttact tctggaatac
    17101 catgtattag aggttgtgaa aaagctgctt agtttcaagt agtaagaaaa attttgtgaa
    17161 aatggctttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    17221 ccgtttatcg agaggttgtg aaatcagcgt cctgacctga gtattagaga aaaaaacgga
    17281 aaaatagttc ctcatatttt gccgtttgtt gagctaatac cgagggtcag ctgattgaac
    17341 accgtttagt ggagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaat
    17401 caaaagcagc aaggagaacc tcatctataa aaagatagaa ggaagtgaga catctattgg
    17461 tctcatttcc ttctattttt actttcctca gtcaattgtt ttacatcata aaaggagaaa
    17521 ttccacttat ttttttcgct gtttcatgac ataaacaact acaccgatta acaaaattcc
    17581 tccagctaat gctagcaacg ggttattaaa ttctcccgtt tttgggagtc cttgagtgcc
    17641 atttttttca ctttcttttg ctgtcctatc cggatgagaa ggttttccat ttggttttcc
    17701 atttggtgat tcatttcctg gattttctct ttctggtttg tcatttcccg ggggcacatc
    17761 ggcagtttct cgcacagtga tcgctttttg ctgggtaaca gattctgtat gagcagacca
    17821 tttttcccca tcaaatattt ccgaacgata agtgacttct agcgtatatt ctccttttga
    17881 aggtgctgaa aaaatagcgg tatgattaga aaattgagca cttgactgtg tttcaacgat
    17941 ttggatcttt tctgggaaat aacgcatatc ttcaaagaca ggcgttcctt gatttggtgt
    18001 gaaagttgac acaaaaactg taaaaggctt cagttcaatg atatttcctt ctgcattctc
    18061 cgccacacca tttgcttctt gtataataat cggattattt tgttcatttg gttttgtctg
    18121 atttggttct tgtacgattg catactctgg atattccgaa gacggtgagg tttctccgtc
    18181 aaaaatagca cctacagata atggaagacc atccactctt tccttttctg tttgacgatt
    18241 ccccggttta atctgcatca gtccatgaac atggatactt ccattttcat agcgcgtaac
    18301 aggctgctga ccttctttcg aacatgtatt tagagactcg atccaatctt ttgatatggt
    18361 atgtccaaga gtattttctg attgtttctc tcgttcattc caatagtagt tcaacgagtg
    18421 gtagatttga tcttcgtttt gattcaatgg gcggatctgc tctctcatct caagattctc
    18481 tgttcggaaa gataagatac cagaagcatg gtagtctgtg tttcctgctg aagaggtata
    18541 aagagccaca ttatatgaac cgttattaaa acttgtactg ctattgacga taatatctgt
    18601 tccgctattg gaatcgattc ctttcgcaag attttcgaaa gacaaactat tgatcaattc
    18661 atgagggccc gttaaagaag aaccgcccat tttgaatcca ttaccgttac cttctccctc
    18721 aattcctggt acccagccgt ttcgataagc aactgagttt ttgatgatta cttttccgat
    18781 cgaacctgtt tcatttttag caaaaaggtc ccaaccgtca tctgcattat gatacgcgat
    18841 acaaccgtca aatacatttc cttctcctat tgtcagtttg gccgcaaatc catcggcatc
    18901 ttcaaatcct ggatcggcat tttcaaaaga tgtacagttc ttgactaagt tatgcgcggg
    18961 ccatgcttcg aaaggatcat ttgagctacc gctgatttgg attcctgtat ttccattccg
    19021 ataagcatga atctcttcga tcaaattatg atttccagaa atctgtaatc ctttttgcat
    19081 agctgcacta tttgtaatgt caaaccctcg taaaccccag tagtgactga acagtgtcat
    19141 cccatttcca gttcctttaa aatcaagtac aggacgcttg ttttctggat ttttctctgc
    19201 aatcaactga atcgggtttt ctcttgtccc gttaacgcct tttggaatcg tcaatcctct
    19261 ttcgaaggaa taagttccag gcgccatgac gattgtctgc ccagcatatg cgtatctcaa
    19321 tgcttgtgtc agagataatg gattttgtct gcttccgttt cctgcatctg acccttctgg
    19381 tgttacataa actgtcgaat atttaggttt acggtagtcc actgtatgaa gaatctcttt
    19441 tgtttcataa ctagtcatct ctacgtattc ttctggcgga tagtttttat ctggcgtaaa
    19501 actgaaagta aattcgttcg ttccttccat cagttttgtc tgataatcaa attcccgatt
    19561 cgcagtgata gccaagtttt ttgcgttctt aagcacctct cctgttgctt tatttttaat
    19621 tgtcaataat ccgtcagcat ttgctctaaa ggacatagta tgatcttggt ttcctgaatg
    19681 attactagag gtcacagtgt aaatcggatc aatcaagcga gtaggtcttt cttgcccagg
    19741 aggatctgtc gcaggatctg agtaagcaac ttcgattcct tctaccttga tcgtcatatt
    19801 ccttgctgca gcaaatcctg cataaataac cgattcatct gaagcgtata atttttccca
    19861 gtcatacatg atgtcagaag ccatttcttc tcctgtttca ggatcgtaat aatatgcctc
    19921 taaaccagta ttcgtttttt tcatctctac gtccagttta gtaatcgagt tcgtggccgg
    19981 aaacagattc ccatgaccgt ttccgaatat attataagaa cctgtttctt tttccaagaa
    20041 ccctgctgaa gtctctaaag gtgttgtttc cactgagaca ctcccaggag ctggcgcagc
    20101 ttgcggatct tcagatgata ttcctgtgat tgcccgtgtc cccaatccaa gtcgcatcgt
    20161 atatttgctg ccgctcgtgt tgaccacttc ttgtctatcc gagtcccaga catattcgat
    20221 ccgactgcct agtatggcaa atgaatttga ataaaatgcc tgcccaaaca aatgattttt
    20281 tggtactgcg tctcttacca ttaaagcaaa tccttcttgg gcattcgtat aggtccaaga
    20341 ttctatttcg atcgtcgccc gcatcctaaa gttgatgtct ttggggattg cttgataata
    20401 ataggtcatt ccatctgatc cgcttgactg gaattttccg ccgttattcg tggaagagag
    20461 ccgcacgccg tcttctaatg gttcaatggc atacctacta gcacttgtgc tttctccgaa
    20521 aacaacccct ttccactctt catttgcttc ttcagattgc ttttttgtct tttcattttc
    20581 tgattctttc atcataggat catttccttc ttccgtatcc atatcttcac tagttcttgg
    20641 tatttcctcc attgttgaca tcccttcaat gttcacgtgt gcattttctt cgttcacaga
    20701 ctgttgttcg ttagcagcaa cttcaacgac agaaattaaa accggggtac tgagcataag
    20761 gagattcatc attagcgcac tgtattttat acgttttttt aacaagtgta ttcctcccgt
    20821 aacaaattag aaagcgttta cttaaagtat tacctaattt atttttactg tcaataaaaa
    20881 acaaaattat aataagtgta ttttataaaa agaaaaaata taacttaagt tgttgataat
    20941 aaagagattt attaacggaa aaagaataag tttttttaaa ataacgtata aaaaagagca
    21001 taaaacaaag tcgtttgttt tatgctcttc ttttattatt ttgtgctttt tattttatca
    21061 aatcactttg tttttttata aaatttctgc gcctagtgtg gactcaaaat gtctcaatgc
    21121 ccattcatgt ccaacgggat cgaaacttgc cacggaatct ttgaaaatat ggagtttgta
    21181 tcctagatta tacgcatcga ctgctgtatg caacacacag atatctgtac atacgccggt
    21241 caaataaata tctgtgatct ggcgttctcg caaacggatg tctagatctg ttccactaaa
    21301 agcggaataa tgccgtttgt ctatccaata aacattttcc tcttgctcat agtcttggta
    21361 taaacttttt aaagagccgt acaattcccg tccacccgtt cctatcacat tatgcggagg
    21421 aaataagcgg ttctccggat gaaaggaatc ttttggatca tgcgcatcaa tggcaaaaac
    21481 gacaaaatcc ttttgatcaa taaacttctt tgtatgagca gtcagctttt tttcaattac
    21541 ttgtcctgct gtcccagtag tcagctttcc atcctctgca acaaaatcat atgtgtagtc
    21601 aatcgagatc aatgcgttca tcttagtcct ccttatacga gatgtttgac aaattcgatc
    21661 accatttctg ctgatcgttt gcctgcgtct tcgataaatt cgtcaaagct ttgtgtcgcc
    21721 gagtgatctg ctgtgtcgct cattgcacgt acgatcaaaa atgggatatt gaactgacga
    21781 gctgtttgtc caacagcggc tccttccatt tcgcaagcta atgcttcagg gaaattcgtt
    21841 aagatttctt tgattttatc tggcgagtcg acaaatgtat cacctgtaac gatcaatcct
    21901 tcttttgcgt tcaaattcgt tgcttttgct gctttaacca tttcactgcg caaatatgtg
    21961 cttgcttcat aatatagagg cattcctggt aattgtcctg ggttgtagcc aaatcctgtt
    22021 gcatctgcat caaaataagc tactttgtct gatataacga tgtcacctac ttgtaagcct
    22081 tcaccgattc cgccagctga acctgtattg atcaccatgt tcacgccgta ttgttggatc
    22141 aacagacttg tggtaataga tgctaagact tttcctatac cggaacgtac gacgatcact
    22201 tcgtggttac ctaatgaacc tgaaataaat aaagcgcctg cgcgttccca tgataatggt
    22261 tcgcttaggt tctctctcaa aatttttact tcttcttcca ttgcgccgat aataccgatt
    22321 ttcatctttt taattccttc tttctatata aatgcgattg ctaaaaaaac cacacataac
    22381 attaggataa caataataag agatttcatc aaaaaagagt tccatttggt cgtcttttcc
    22441 cgttcgctga tccttgtttt cgtgattggt ttattctttt tgctttcttt acgataaaag
    22501 ctggcaatct ttttttcttc ttgctgataa gctgtttctg cttttcgctg tttttttaat
    22561 gactcgcttg cttgtgcttg ttgtcgcttg cgaagttcgc ttcgcgtaat aagcggtcct
    22621 ttggccataa gccgatccct ctactttcct ttggtcaata gttcccagta ttgaatcgca
    22681 taaatcgtct ttgcatcaca aatcaactga tctttcatcg cttgttgcgc ctcttcaaga
    22741 gttagatgat acaactccag tacttcgtct tcatcttgtg ctaaaggatt ctcgaccttc
    22801 tctacccctt gtgcatgata aatatgaagg acttcatttg caaaacctgg tgaaagatac
    22861 atcgaagtaa gatggctcag cgacttcgca cgatagccag tttcttcttc taattctcta
    22921 gctgctgtca tttcgggatt ttgtccttct cctggatcga tttttccagc tggaatctcc
    22981 aaaatcactt tttctaaagg ttttcgaaat tgtttgacaa gaagcagacg atcttgttcg
    23041 tcaaaagcaa tgatcccgac accgccagga tgaaatacca gttctctctt cgaagttcct
    23101 ccatctggca aacgaacttc gtctacagca acatcaataa tttttccgct atagatttct
    23161 ttgcgactga ttgttttttc ttcaaattgc tcattgttca gcatatcttc tcctgctttc
    23221 tttgtaatca taactgcttt tttttcattt ttcaattttt ctcaaaaact ccttattagt
    23281 ttaccataga taaaccttag attttctcta aatatagaaa atccttcttt agtccgatac
    23341 tctttctagt agtccgtatg ctaaaataag tatgtaatcg aataacgaaa agaattcaat
    23401 actcaacgat aaatgacatg gagggtttat tatgataaaa cgaaattggc cgtggcttct
    23461 agcattggtc ttgctatttc tctttgtccg aaacaatagt ttttcttttg ttctaggact
    23521 tttattgata ggtgtagttt tagttttgtg gggactcttt ggttcgcgca ggaaaaaggg
    23581 aaaacaagaa gaaattcctg cactgactga cgaacttgaa actcactatg aaaaaagcgg
    23641 tatgacagcg aatgaaattt cattttttcg acaaacaatg aatcaaacaa aaaatgagat
    23701 caatcaattg caaatgaata tgcagcagac agccaaactt aaagcaatcg atctccgcca
    23761 tgatccagtc aaagcagcga aagctttatt caaagaattg gtgaaagaac caaaccgact
    23821 gcatgaagca agtcagtttc tttatacgca tctgccgaat ctagttgatt tgacaaataa
    23881 gtataatgag atcaatgacc atgagatcaa aaacaaacag acctatgaaa aactagaaga
    23941 aagcgcgcag atcatcgacc agctgtcagc tttaatcgca caagattatc aaaaattcgt
    24001 tgctgaggat ctagatgata tcgacgtaga gatatctgtt gcgaaacaga gcttaaagcg
    24061 tgataataaa tatgaaccag aacaaaagga ttaactcttt tgttcataaa agaagactcg
    24121 gaacattcag atgatgtctg gctgtttcga gtactttttc taaagtagag acattcatgt
    24181 aagaatcgag aacagattca acaaggagga attttttatg gatcataaac cgactgaaac
    24241 aaaagatgtt acaccagtta acgatacact tgaagattta ttgaacaacc cgttttcaac
    24301 tccaacagat acgctgacca cttcacaaca agcggagatt gatcagctcc aagagcaaca
    24361 gacagctgct cgtttaattg acaaactacc ggcggaacgc caagaacaag ctaaacagct
    24421 agcagcaaaa atcgatgcta atgatgcaca gtctgtcatc agctacggat cggctgccca
    24481 agcaaaatta agtgaattct cacaatcgat gttgaatcat gtgcaagcac aagatattgg
    24541 tcctgtagga gattccttaa ctgaactgat gtatcgtctc caagaagcca atcctgacga
    24601 actgcgtgcc ggagaaggga acatcttcca gcgtgtcttc gggaaggtca aacaatcgat
    24661 ctatgaagtg acagcaaaat atcaaaagat tggtgcacaa attgacaaga tttcagtgaa
    24721 attagataaa gaaaaagacg gtctgctaaa agataatctg atgttagaac agctttacca
    24781 gaaaaacaaa gactactttg atgcactgaa tatttatatc gctgctggag aattgaaaat
    24841 ggaagaactt caaacaacga tcattccaga agcgatgaaa agagcagaag aaactggcga
    24901 tcagatggat gttcagattg ccaatgatta cacgcaattc ctagatcgtc tggataaacg
    24961 tacgcatgat cttcgcttag ctagacagat cacgatccag caagcaccac aaatccgttt
    25021 gatccaaaac acaaatcagg ccttggctga aaagatccaa gcatctatcg ctacagccat
    25081 tccgctgtgg aaaaaccaag tcgtgatcgc attgaccttg cttcgtcaaa aagatgcagt
    25141 aactgctcaa cgccaagtct ctgaaacaac aaacgactta ttgaagaaaa attcagaaat
    25201 gctgaaaatc tcagccatcg aaacagcgaa agaaaatgaa cgtggaatcg tggatatcga
    25261 gacccttcaa accactcaaa atgatttgat cgaaacaatc caagagacat tgcgtatcca
    25321 aaaagaagga aaagaaaaac gtcgtcatgc agaagtagag cttggacaca tggaagaaga
    25381 tctgaaacaa aaactgttgg atttaaccca atagatcacc tttcgcttgt tactaaaaca
    25441 aacgcccaac aacagtttat tacaactgtt gatgggcgtt tttcttatct ttcttctaac
    25501 ttctccattg gagtctattt ctcattcatc ttgctgtgtc catagccata gccaaaataa
    25561 atgatcagtc caataaccat tacgataccg aacacgatcc atgtcacagt ttgcagacga
    25621 agcatcaata caatacaagt caagatggag acgattggca atagcggaac gaatggcact
    25681 ttgaattctc cagcttttgg tttgccaaac atttttctca acttcagtaa gcctagacta
    25741 acaagcatca aataggcgag cgccatgata ttcgttagtt ccgctaatac attcaatggg
    25801 aaaaatccgg caaagaatac agctgccaca ccagcggcat aagtcgcttt tttcggtgta
    25861 cgatgctttt gatcgatttc tgtcataaac ttaggaagta agccatcctt gctgacagca
    25921 aacagtaaac gagcaagtga atacatcatc gagatcgtta ccgtcagtag cgttaaaatt
    25981 gcccctacag cgatcaccgc gcccactgtt ccatgtccta catacctcat tgcaaaagcc
    26041 actgggtcat tgactcctag ttcagtatac ggaacgattc ctgtcaagat cagcgtcacg
    26101 atgatgtaaa ggacagtagc aatcagtatc gaaccaatga ttcctcttgg cacatctttt
    26161 tgcgggtttt tgacttcctc ggctgccatt gcaacggcat caaatcccaa gaaagcaaaa
    26221 aacacaagag ctgcaccatc tagcacaccg gaaaaaccaa aaggcatgaa tggctgccag
    26281 ttatcaggtt taacgaaaaa tattcctaca atgataaaca acgcgatgat accaaatttg
    26341 acatatacca tggcattatt caaacgtaat gctttcttgg cttcaagcga cacgatgtaa
    26401 gtgacgaata tcactacgag caccgcaatc aagtcaatgt atgtaccatt ttccggattg
    26461 tacccgccag ttaatgcttg tggaagttcg gcccctaaac ttttcaaaaa accttgcata
    26521 tagccagacc atccggaagc aacagaggaa accgctagta aaaattcccc gatcaacaac
    26581 caccctgtca gccaggcagc aaattcacca aatacaacat acagataggc atatggccca
    26641 ccaatcactg gcactcgcga agcaaattct gcgaaactta gtccagacaa aatgacaacg
    26701 attgcagcta agacaaatga aagtgacagt gctggaccag cattttgatt ggccgcaacc
    26761 cctgtcacca caaatattcc cgtaccgatg atcgcaccaa ttccaagcat gatcaaatca
    26821 gacgttttta gttctttttt catctcactc gtatgattga gattgatctc tttcttacga
    26881 aacaaatcca ttgaagttcc tccccatttt aggttggaaa acagcgatta aactgttttc
    26941 atatacccta aaaggatacc acctaaaatg actaaaaggc aaccaacaat aacataaatg
    27001 agctcttttt tcgtttttct ttcgccaagg atgaaaatcc cacctaaagt agagataatg
    27061 atccccattt gtgaaagtga gaagccaaca gctaaaccaa gtgatttgac tgttagaagc
    27121 atgcagatgt ttcctaagcc ccataataat ccacagatca tgtttttcca cacaaactga
    27181 tcgactttaa ctttacggaa agcaaagaag cttgcgccta agatcattcc gatactttga
    27241 ggtaagataa ttgacatcgc atccaaattc gcccagttga cgatgatggt atatacacca
    27301 taaccaatcg tagagaagat caatgctcta aaacccttac caaaatcaag cattttatta
    27361 tctgtatcta tttttccttc gtcatcttgt cgggcagtca aatacgcacc gcttacaaga
    27421 agagctaaag caataatacc gtatacataa tctttcgttt gtgtccattc atggaagaaa
    27481 acggctcccg caatcgtatt caaaattaat tgaaaaccag tagacaatgg tagccctaca
    27541 gaaacaccca tgtacttcat tccatggaac tgtccatttt gtccaacact ccaagctaag
    27601 ccggataaaa atccgatcaa gaaaatccaa ggggtgatta caggactaac aacgaaaaag
    27661 acaactaaag aaaacagaaa tgcaccgatc gtcattccta gtgtttgttg attggcatcg
    27721 ccaccaattt taccacttac taaaccaata ctaccccatg ccaacatagg gactaatgct
    27781 attaaaattt ccataaccaa tctcctttta tttcacatga taagaaactt atcagcgaaa
    27841 acgttaacaa cacaagcact ttttttcgtt tctaacgatt ttctaacgtt tttcattata
    27901 aaaatttaag attgggaaaa ataaagtcat tgttgtccct tttccttcag ttgattcagc
    27961 cgtcaaatgg atacctaatt tttcggccag attttttgct aaataaagtc ctaatcctgt
    28021 tgaatgtgtt ttgctaagtc gaccatttct cccagtaaat cccttatcaa agatccgttt
    28081 caagtcttcc gctgggatac cgatccctga atctttaact gaaaggctga ctccttgcgc
    28141 ttccttttct atgctcacaa tgatgtttcc gtgatccggc gtatatttga tggcattact
    28201 aagcaattgg ttgaagataa aagcgatcca tttagcatcg gtcaaaacag tatgatcctc
    28261 accaacgatc tcgtagcgaa tatttttttg gataaaataa tttgcctgac tgcgtatcac
    28321 aggctgaatg attcctttta gtccatattc ttggatgagg tagtcccgcg agaaactgtc
    28381 gagtcttgca taatacaaaa cttgttctac gtattcatcg atttttgata attcattttc
    28441 tagtaatgta tacttttgat cgtcaatatc gaactcgatc gaacgaacta gcaatttaga
    28501 agcggaaagt ggcactttga tttcatggac ccatgagtca atataatctt tttggtcttg
    28561 ttgattactg attgctgatt gcattacttc ttggtgttca cggatcagct gattgatata
    28621 ttcttctacc agtttttctt cttctgtcct cccaccactc aaataatttt gcaaagggga
    28681 agttttttgt atgtcggcta attttcccca ccatcttctc ttgctcaagt aaccacccag
    28741 cagataaaaa atcagaaaga cgccctcaat caaagctaaa taagcaattg tgctcaaatc
    28801 aactttgatg tttggcgcca gccacatcac aaagatcgtc aatgcaataa aaaacagcca
    28861 tcccaagatc aatgtccact gatctttcaa atatttaaaa aaattcatct taaccgctca
    28921 tcctttcagg gaacgatata tccctgtcct acttttgttt cgatatatcc ttggatacct
    28981 gcctgttcga tttttcggcg taaccgattg atgttgactg tcaatgtatt gtcatctaca
    29041 aatcgttcgt catcccacag tgctcgcagc aatttttccc gacttaagat ctttccattg
    29101 tgcttcatca aaataaataa tagacgatat tcatttttgc tcaaatcaat gatctctccg
    29161 ttgatttcca tacttccgtt gtcaacattc aacgtaatgc cattgtggct catcatttca
    29221 ctagataatt ctgtgtagtt gtaagaacgg cgaagtaatg cattgatttt tgcaatcagt
    29281 acatcgattt ggaaatgttt tgtcacaaaa tcatctgccc ccatgttcat agccatgatc
    29341 atatccatat tcgtattcct gctggagata aaaatgatcg gtactttcga tacttcacgt
    29401 attttttgac accaataata gccatccaaa acgggaagat taatatctag taatacaagc
    29461 tgtggttctt ctctttcaaa gtctgagaac acttgattga aatcggtcgt tccaaaggtt
    29521 tcaaattgcc atttctcaag ttcctccatg atcaattgtc gaatgatctc ttcgtcttcg
    29581 actaccatga tttttgccat aaaaaatccc tccatctttc ttcttgctac tacaatatca
    29641 aagaagccct tgtgaaacaa gttggcttac ctggaaaatg tcattttagc aaacgttaaa
    29701 aaaaactgga aatatgcgat ttaatcgcgt ttgtctcttc caagcagctt aaatttatta
    29761 tgttattctt aataattggt ctattatctg ttatttttct ttcgttgttt ttgtctttta
    29821 aattttcgat aacaccaata accgaaaagc gttagcacta gcaatatacc tagaaggagt
    29881 ataagtaatc gataaagatg gtattttttt gtctgttcta tctttgaaga gacttcttct
    29941 gtaaagggaa ctcgtcttcc tgtcactagt aatcggtgag tgttcacggt gtaaggtgta
    30001 caagtgataa gtgtgactag atcctgcttt tctctaattg ctaatgtatt tgtttttgtt
    30061 ggaaggacaa caattttctc aacgacttca taagcgagca tccgaccata cacttcaaga
    30121 tagaatcggt catcttgctc catttcttca agatccgtaa agagtttttt ctcagcaata
    30181 ccgctatggg cggtaatcac ggaatgtgta ctctctcctc cagttggaaa agaggtgcct
    30241 tgcaggactg tcgctcctct atctaacaaa agatcattcg tttcatcaaa taaaggcaag
    30301 ctaacattga ttttcggaat atagatggct ccgatcatat gttgttcgta atattctttt
    30361 cctggatttt ggacatttct aaccgcttgc tcaaaaggat ctttgacttg tcccattcct
    30421 gggattactg atgtatgagc aagcgtctga tttttctttt catgcactgc taattgttgc
    30481 tcatgttttt cttctttatc gcgttcaagc tgcttttgat aattctcgat ccttctttga
    30541 tccaaataat tgctgagagc atctgctaaa aatggatatg taaaaactgc agctccggca
    30601 agaaacatca ctgccataaa taattgtaat aaaagactga tttgggtttg aggtctatta
    30661 cctattttct ttttcatttt cttcaccttc ttatttaaaa aaactgcagc aacctttata
    30721 aaaccgattg ctgcagctta tttttgttgc ttatacttca gacttcattt tagatttacg
    30781 gtaccagctg tacgctccaa tcatcaaact tattccgatt agtaagaatg ccaagagccc
    30841 gttcccacca gtagcaggaa gtgacccgcg tttcatattc ggtactcttt ctggccctgg
    30901 attttctact cctacgatct gaatttcttc attatagctt ccataagaga tgataaattc
    30961 agtaaaggct gcctcacctg ttggaagtac ataaccttct ggcgccatgg tctctttcat
    31021 ttgataggtt ccataatcta acccttgaac ctcaaagtat ccatttccat ctgcgacaaa
    31081 ttctgttgct tgtccgcttg ttgcccactc aattctatct gctcctgatt cataaacagc
    31141 taaaggcgac tcccctttat aaaatattgc gtattcagaa cggtcttcat tccacaattc
    31201 gaatcttgca tctttaagca attcactgct tgatgcatca tatttttcaa actttcttcc
    31261 acctgttgtg actttctctg gcggctctac tccttcttca taatcaaatt catcatttcg
    31321 tccgatttca ataaccgctg tgttgttatg gaaatcatcc gcttctagtt ctgtggaaac
    31381 cttcaaatta tacgtgatcg ttaaatctcc tccacgtgca tctgctaatc tacctaaagt
    31441 atctactttg gctactccat tttcttctgt caaaatcgtc acgacaaacc cgttgcttac
    31501 tacttctact atataatctt gtccttttgt taaaatcgtc tcgccggctc gcacttctat
    31561 ttcctgatca taataagtta gaccaactgt aggcgtatct gtgattttgt aatttttgat
    31621 aacagcgctt ccgttttcaa ggacagaatc gatgaaataa ggaataggtg ctgtgatcgt
    31681 gtaagaagcc gtttttcctc tttctagatc aatataagaa atcgtttctt gttctccagc
    31741 ttcattaata atcgttactt gtctaaggtc gctttcctct acattcatct gtttatctgc
    31801 ttctctgatt tcgtttttcg gatacaaatg cacatctgta ttgtatgtgt ctttccattt
    31861 agaaccatcc cacacgccat ccaccatatc aggcatcatc actggcatca taacaaccat
    31921 tggagacgcg attctggaaa tatgagctgg cgaataagtt tctaagaaaa gatagacttt
    31981 atctctagaa gaaggtgctt cttccgtcac ttggacggta gagaataccg cttctccagc
    32041 ttcattcgtt gtttgtttgt caatacttgt cagtcgatat tttaaaaacc aactttctgt
    32101 gtgccgtttt tgaatccatt caatcgcttc ggcagaatta agaccgtctt ctcgttccgg
    32161 tgttaaagga ttatctcgaa tcaaatcgta atatacttcc gttacgtcaa acaaatcaaa
    32221 atcgactcct ggaatatttt cttcaccaaa atccgtagaa accaatccgc tattcggtcg
    32281 ttcttccgga acagatgaga aacctttttt gtgtaatgta atcgtcactt cacttggttt
    32341 ttgtgcgact tcttcggctt tgactagttt tccgttcccg aaaatcccta ttcccgcagt
    32401 aacgagtaac agtacactca ttcccgtgac aatcattttt ttattcattt ttttctcccc
    32461 gttctttttt tattaataaa acagcaccta acaataaaag aattcctagt aaagaaatca
    32521 acgttcttgc ttcattcgtt tgcgggaata cggaccattt tcctgattga cctggattgc
    32581 ttggaccact cggctctttc ggtttgtccg ttgtttcttt tacggtctcg ttataaacac
    32641 gtggtgttgc ggattcatag gcagagactg gaactttccc ggattctaat tcagccatct
    32701 cctgtccgtt aactactaca tattgtggtt ggccttttgc atccacccaa aattcaggga
    32761 tgatgacctc gattctctta cttgcttcat ccatctcgta accttccaca ccttttaatt
    32821 cttcaaaata ataggtgcca gaaggcaaga aacgttgtcc tgtcgtaaca aggccctctt
    32881 tatccgaaat gaatttgctt acatttttgt cattcaaagg atcattgtct gctggtttga
    32941 tccattgatt tttcaagtca ttagcaggag ccatatctaa ataatatttc cccccttcta
    33001 tcatctgata tagagcaaaa acagcacctt ctaatggttt tcctttttca gttgttcctc
    33061 tttgacgtcc atacttgaag aagtatggat ctcttagata accgacattt ttaggataga
    33121 tatgaatctc ttgcagttcc tcggttgatt ctgtcgggtg ataaattggc aaaatagcag
    33181 caattgggat agctgtctga tcgatatcaa catccaatcc aacttctgga tcaatcgatt
    33241 cttcgaaaat caaataaact gcatcccgat cttggttttt cttaggtgct ttgatacgtg
    33301 caatcccgtc tcctaatccg ccaaccgtat ctttcccagt tgttaccgaa gtccctattt
    33361 ggacaagcct ttcttctgtg attagctgac ggatattgtt tctgttcgtt tcagcgattt
    33421 gctggacgaa ttctttagga gtcatgtttt cagcagcttt tttttcatga taataatcgg
    33481 tcatgtcgta aatggcaaat gtcgctccat ttagtggcac cgtctgtgaa atgatatctg
    33541 ctgtttcccc tgcttcctca gagatagcca agccatcatt ttcatggtat tcaaattgat
    33601 cgttataatc gatatctcga atcatccgtt tatgcaacac gaattccacg acatcttccc
    33661 ctgattcttc tgccgacatc gcatgtggaa gaaggactgc tgcaagcggt agtaaaacaa
    33721 gtaattgaag catcaaacac aacagctttt tcattacctt gctccctcct ttttagattg
    33781 tctgacaaac aaaccatagg ctgctcctgc aagcgccatg aagaatgcag caagcaggat
    33841 atagaataat cgtccggatc ctcctgtagc aggcaacgga ttttcaggat cgttcccgac
    33901 actaagctgt aatctgattc gatttgtctc caaaagttca tattctaact cttgctgtag
    33961 atcaggcgtc tctgcttctt cgtttggata atacgaaatc tcaactttta gtgtacccgc
    34021 ttcttttcct ggaactaccc gatcattttc tatgtcaggt tcttctcgat aactaatttc
    34081 taaaataaag taacctggaa gttttctata gctgtcaggt gacttcactt ccttgatccg
    34141 atagacccct tccaacaagt ctgaagcatt gaatagggaa gaatcgtttg gatctggtgc
    34201 caagcctccc tcgatgattt catactctcc tgcactattt tgtttctcaa ttgaaaattc
    34261 tgcgccttca agacgtcggc tattcatact gtcaattttc aaaagttcca aatcgattgg
    34321 cgctagttca tcttctaagg ttacttgtag tacaccatcg atgatttcat agccttccgg
    34381 caagggagca gtttcgtctt cgatgaccgt atcttcaatg ataaacttac cttcttcatt
    34441 cagtgcaaac tggaacactt gatcagatag ccgataccca tttggtgcga cagattccga
    34501 gacctcatat tcaccaatag acagatgcag aggttggtcg taatttaaat cgacatactt
    34561 ttctactcct acggcacgag aatagaagta tccgatccca ggcaaacgtt tcgattcttc
    34621 tggttctaac ggattaccag tgtatgctcg atacagcggg aaggaagaat cccccacttt
    34681 cctgatatcg aaacctgcac catctaatgt atggccttca tggtctaatt ttctaactcg
    34741 gatctcccga taatcaaacg tattgtaagc agtgaatgct acccataaac gatcctcttc
    34801 atctgttgta tacgtgagat attgccaatt ttcttcacct gtttgcttga gttggatagt
    34861 ccctatagca ttatcgtaaa gtcgcaagat ccatgtgtca ttttcatcag gttctttgaa
    34921 accttctggg ttctggctct caatgatttt gtattctgca tagttggtag tttcttcact
    34981 atccttataa acaaatgaat ctggctctcc gattggatga ataactttct caccatccac
    35041 gattacatat ttcccgtctt catctactga atagaacgtg agtctccctt tctcttcctc
    35101 tacttcgttc acaccagaaa gccctttcgc tactggatat ccgatttcca gtccgttacc
    35161 atctgtttca taaagttcaa actctgcgcc attgattggc atattactgc cttcaattag
    35221 tttattgata ttcatgtgga ttggcttgaa ttcatttcgc atttccagtt tgacatacaa
    35281 ctcatcatac tctgtgcctt ctatgttagg ctcttgtaca acagaaagct tgtgatattc
    35341 tccttcataa taggaattcg gatctttacc ttccggtccg ccgactctcg aaccagatgt
    35401 atattcatct acaagctcgt agtgacctaa catctcatct tcgttggctg catatttttt
    35461 gatttggaat tgatacgtct catctactaa ctgataacgt ggtggtccag cgatctctct
    35521 gatccaaaat atgtcgctga ctgctccagt ttcatctcct aaaatcggca tcttcgtttc
    35581 aaagatcgct attccgtccg tggtagtttt acttgtttcg attggttcgg cttccccact
    35641 ctctggattt tggtcatata atgcaaattc gactccagcc aacggctcgc cattttgacc
    35701 cttcttgctg acttggaaat tacggccttt atgttcgtaa cgaatggaag aagatgggaa
    35761 atacatccgt ccagtagtga tatcattctc ataatcctcc aggttctgaa tataggccat
    35821 attgttcact gtgcaaaaat ttccgtcttg agctcccgca tcaagtttta cttggtactt
    35881 gatcctgacc atttcccctt cacctaaaga aatcggagaa aatttataag cttggttttc
    35941 ccgtccgaca tacgttgtcg gagtcgccac tacttctggt ggggcttgtc catgacgttc
    36001 ttcatcccat atttctggct cttggtactt catgccgttt tctcctggat ctagatggta
    36061 aaactgaata tctgccgatc tgccagctcc aaatggtgag acattaaaac cagtttcatc
    36121 ataaagtgtg gcttgctgga tcgttctctt gaaggtagaa gagatatcct ccaaggctgt
    36181 aacaatgtca ttcttgttgt tcgcattgta atatcgcggt gttacgccat cttcttctaa
    36241 atcagcaata tttttcaata catttcgtcc aatagcatct agtcgttgtc tcccgctaac
    36301 gtttccatcg attcctaaac caatggagaa gatacttggc gggttctctt gatacttatt
    36361 tttcagccaa tgatgataag caactgtgtt gatcgcagat gacttttctt gcgaagtagg
    36421 actgccattc gtattgttat tagaagaacc atcaccaacc cagtgagtcg ctttattatc
    36481 tccttctttt acctctccat aacgatagtg tacatttact tcatcttctg gacggttaaa
    36541 gtcagatgca taggtaactg gatgagtaaa ccctgcatta gaagtattcc cagaaaactc
    36601 ctcaaaattt ctgaacaaaa cgtcattatc ttcagcaatc ttatcttcca tcacagtcca
    36661 gttgctccaa gctccattag tgctcgtacg ataacttgac tgaatcggtg cataactgaa
    36721 ggtcggtgtc ccatctccca caacgattag gattttttct ggatttctat tttctccgcc
    36781 attgtcttta tacaatgttt catagccatt tttaagccct agtgtaagcg gagttcctcc
    36841 gattggtgta cgatttattg ctgtattctc acttcccgtc aaacgattga tatcatttgt
    36901 caatggaaag atgtccgaac tgatttgctc atggttattg atagctgtac tattatggta
    36961 aaaattcacc ataccgattc gaatatccag actcgggtca gagagcagat ctttactcat
    37021 ttcgtttact gcctcaatca acgcagcatt tttagtttga ctattggtcc cagcggttaa
    37081 ttcactcata ctcgctgact tatctagaac aaaaacgata tcgattggac tgatttcctt
    37141 tttctcgccc ccgatgacat ctagatagac attgtaaagg gttgttgcat cttcaggatg
    37201 tagatcagat gctgtgctgc ttggcagaac aggttctacc cattttttga tgtacgcccc
    37261 gttataatcc aagtaactgt tttcaaaatt ccgttcattt ccccaaagct gaacggtttg
    37321 agctggaacg tttctatctt cttcgcctgc agttgatcca ttattagtag catagtcgta
    37381 atttcgccag ttcgctcccc cgtttgtata atcactatca tcgatcaagt tctctccgtc
    37441 atatggcgag cctttgtctc cttgatagcg ttcattgctt tgtgtccaat ttcctaacac
    37501 ttttgtatag tcattcgttg taaacttcgg gtagcgatgc tgttcgtttt caacttctcc
    37561 gctaggatag atatattcat aggggtcttg cgtagctgtt ggcccaagac gctccaaaat
    37621 accgttgaat aactggcgtt gaatgcgctg gagtgcttga ccgctgggta cgattggttc
    37681 gtccagcttt tccatctcat tttcttcgct acgttgaatt tcagcggaat cttctgtatc
    37741 agtttcattt tcttgtaatt cttggttatc tattctttca tcaaccacca cttgaagagg
    37801 tagttaccct tcgttcacat cggggatctt ttctgtttca aaaatcaatg tgccggtttc
    37861 tgcttctttt gaataaactt ttccgacata ccattcttga ccatcgatcg tctcgacttt
    37921 taggtcttct ccatcttcat cataggagga tagacctgtt ccacgaacgt tttttacggt
    37981 accaatcccg gcggctgccg catcgattgc taaacgtatt ctaccttcat tggcactttc
    38041 ctttttttca aaagccagat gccattcaag ggtttcttct ttttcaatat acttcccttc
    38101 aatttttgca tagtcctcat ctaagaatac ctcatcctcc tttgtttctg ttgactcagc
    38161 agctacatcc agggtcgtca tatgatagac tgactggaaa tatggtccaa atagcaaact
    38221 gacagttact aactgcaaaa gcttcaattt taaccctttc acatccttat actcctttca
    38281 aaaatatttg tcttctttca caacaatcaa caaaacaatg cccatctaat tataatttaa
    38341 tttaatttta attttaaatc aaatgatatc ctttttagtt aaaaaacacc aattatacaa
    38401 catttattat taaaattaaa ttataaaatt ttataacgtt ctcatttaac aaaacttaag
    38461 ttattaagac aaatgaataa acaactacgc tttcctcgaa acaaaaaaat aaagcacaaa
    38521 tgcgcattta cgcactcatg ctttattcgt acaattaatt cagataacgg cagcgcatag
    38581 gatgccaata acacttccta aaatagctcc tcctaaaatg tcagtaggat aatgtaaaag
    38641 taaaaaaatc ctcgaataag cgatcaaaca agcaaataaa aaggctaata ttcctgcaac
    38701 tggacttaat tcgaacaacg cagctactga taaaaaggct agcgctgtat gtcctgaagg
    38761 aaaagaagaa gtcttcggtt tacttgtgta aaggacaagc cgacgatttt tttcataagg
    38821 tcttttccgt cttgtcgttt ttttgataaa aagcaaacca acaaaaaaat agatcaaact
    38881 gccgataaga atggctagac catattctct agtatcaggt atcaagacta aaacagcagc
    38941 aaggagcaac caaataaaac caccattaga gatattactg ataaatttca aaaaattgat
    39001 ttttcgacta tccgttttcc gttgcttggc gaacttgttg ataatactat catcgatttt
    39061 agcaatcaaa cgcaacatgt tattcctcct ttttaagaac cttttcttgc gtacgatgga
    39121 taaaaagcag gacttgaaaa taaaacaaga aactgaaacc taataaagca aaatagattg
    39181 gtcctgcaaa ccagaatgcg acaagaagga tcaacgtgcc tgccaaatag aaaagcgcgc
    39241 ttttgaaatc attaaaaaac aagaaaaatg cagtaatcca agaagcttct ttttgttcca
    39301 gttgaagcaa gattttgtaa ctaaagagaa gcgcgcttaa tagaagcaaa aacaacgtag
    39361 tatacatcaa tgtatagcca attcccccac cccaatagcg gaaaatctga taatcgatca
    39421 atagaagaag aaaataaaca cttgctaaac tatcttgctt tccataaaga gagaagttct
    39481 cacgaaactt tttccagaat agtgaaccga actttttctc ttcatttttt ttgtaatgat
    39541 aaatcatata cagaactgtt cgacttgcag ttcctaatgt gaaaacaggc aagacgcaca
    39601 ggaaccatag tagctgtaaa tggataagac gtggaatcca ttcaatgatt tctaagaaat
    39661 aatcattggc ttttttacca atcgatcgat tcatcatcct tttgaacctc ctgtcaaatc
    39721 catcccttca atgaaataac gttgtgcaaa aaagaacagc aaaatcgtcg ggatcaagac
    39781 aagtgttgct cctgccatca gataattcca ttgtgtcgtt gcttggttct gaaaaacttg
    39841 caagccaatc tgtaacgtgt atttttcttg atcttggata tataacagag gacctaagaa
    39901 atcattccaa gctgcgttga aggaattgat cgcaatggta atcaaggcag gtttcgtcaa
    39961 tggaagcatc aaatgactcc aaatatacaa atgattcgct ccgtcgattt cagcagcttc
    40021 gatcaattca ttattgatcg ataagtaaaa ttgacgcatc agaaatatat tgaacgcatt
    40081 gccgaagaac gatggaacga tcaggggcaa ataagtacct acccagccga tcttggagaa
    40141 taatacatat tgagggatca tcgttacaaa ccccgggatc atcatcgtag aaagaaccaa
    40201 agcaaacatc agctttttcc caggaaaatc aagcttcgca aatccataag caataaaaga
    40261 atttgaaagg acattaccga tcactactag cacagtgatg aataatgtat ttcttgcata
    40321 gcgccaaaaa ggaaaggcgg caatcgtatc cagatagttt tcccatacga ttttttctgg
    40381 gaaaaaggtc ggaggataac gaacgatttc ttccattggt ttcaatgaag taaacaccat
    40441 ccaaagcaat ggaatgatca aaatgattcc tattactgtc atgaggacat aacttagtat
    40501 tttttttgtt tgccgattca cactggtctt tttgaaataa tccatcattt gccgtcacct
    40561 ccagaataat agacccactt aggtgcaagc ttcaaattga caaacgtcag aaccaacacg
    40621 atcagaaaca ggatcattga catagccatc gcataaccca tatcaaaagc tacgaacgcc
    40681 ttgttccaca tatgcagatt ataaaaaagc aaagaatttg caggtccgcc tgttccattt
    40741 ttcgtcatga cataagcttc ttgaaaaatt tggaaagaac cgatcgtaga ggtgatcaca
    40801 tcgtaaaaaa taataggagt gattaatggc aaagtaatac ttttgaactt tccccaaacg
    40861 cctgctccat cgatttctgc tgattcatat aaagatctag gaacattttg taatgtagct
    40921 aagtaaagta gcattgctcc tccagaactc catattttca tcaaaatcaa cgcaggtttc
    40981 gtccattgtg gatcaaacag ccaagctggt ccagggatat ggaaccagcc cagaaatgtg
    41041 ttgatcaatc ctgcagatgg agaaagcagc tgcatccata agatgtagac tgctactcct
    41101 gataagactg ccggtaaata gaaagctgta cggaatacac tgattccttt gattttttga
    41161 ttgagtactg tggctaaaaa cacactgaaa atcgtcgtca gcggtacatt gaagatgacg
    41221 aaataaacgg tgttgtataa cgatttccaa aataaatcat cgtgaacgaa cattcttttg
    41281 aaatttgcta gaccaatgaa attcatcttg gatgtcaaat tataatcggt gaaactacca
    41341 ataactgaaa aaagcatagg ccctagcata aataccaaga acccgataat aaaaggcgag
    41401 ataaataaat aggcccatct tgcttctttt ttctttcgga tagataaacg cttcaaattt
    41461 tcttgcctcc tctgtaccta gacagtcaaa aatggattat tgattttgct tgactaagtt
    41521 ttctactgct ttttgtgcat ctgccaatcc ttcttttggt gattttgttc ctaaaagaat
    41581 ctggtcaatc tgttcattca ctaatgtcgt gtaatccgga gcatctaaag gaactggtgt
    41641 gatcaccgta tttttaaagt tttcgtccgc catttgatag atcatttttc ctttatccgt
    41701 taggttgtca ttattcacta aattctcatt ggctgtcttg ctcgccatga tatcaaaact
    41761 tttttcgccg aatttttctt gtacttctgg agaagaaaga tatttgatga attcataaga
    41821 ggcctcagga tcttttgctc cgtatggtac ttccaagacg aatccgccac cccacgacca
    41881 atggcctgaa ccttcttctt tttctgggag ctgcatcacg ccaaaatcaa aatcatccgg
    41941 tgcattttcc atcaaactag agtaataatt gatattttgg gcacgcatgg ctactaatcc
    42001 ggaaataaat ggatcagata caccagaccc aaattcggct tctaatttat tgatcgtatt
    42061 tttcccataa taatcttgcc agtcttggat ccactccaag gcttccactt tatttttagt
    42121 atcaatcttg actttttcat ctttgtcaaa ccaactagtc ccgttatctg cgtttagcgc
    42181 ccatacatca gcccctaaat tccataatgg ataaaaaccg atgcgtgtaa attcttttcc
    42241 ttctttttta tctagtttac gtgctgtttc ttctaattca tcccaagttg tcggtagatc
    42301 ttgttcactg attccagctt ctttaaataa ctttttatta taaaaaatga cttgtgtatc
    42361 tgtattgaat gggatcgcat aaggatcacc ttcatattct actgtatccc aaagctgcgg
    42421 ataaaaatcc ttagaagtgt ctttttctac atatttggag agattagttg cttgctgtgc
    42481 ttctgctctt tgggcaacgg aatttatgtc ctgtacaata acatcgggtg catttccagc
    42541 tgttattgca gataaagatt tagtccagat gtcaccccat ggctgatact tataatttac
    42601 atggatctcg ttttgtgatt gattgaaatc ctcaatgatt tcttcgatag cttcttttcg
    42661 tgcaccggat ccccaaaagg accagaaatc aatatttttt cttccatcac ttgttttttc
    42721 agaagaattt gctgcacttg aactggcatc gcttgcatta cttgatccgc atcctcctaa
    42781 taaaaacaat gctgaacttg ccagcaaccc tgtaaacaat aattttttca tattttacct
    42841 ccaaatttta atgtaaccga tgccggtttt aatataacga tatcattttg ttctgaaaat
    42901 aacaatttat aaggtatgat tctaaaaaaa ttattaatga taccaatact ttatataatt
    42961 cgcgagcgct ttctttgaat tttttaatct ttcgtagcag aatgagaatg tattaaaaat
    43021 agattaggag gaattttgat gtttagacac cagaaagaat tgcagtttga agcaaaacca
    43081 gatcgtccga atccattaat cgcgaaatat ctgcaggagc tgatcggcgg ccaatatggc
    43141 gagatgagtg tagctatgca gtacctgttc caaggctgga gctgccgagg agaagaaaaa
    43201 tataaagata tgctgatgga cattgcaaca gaagaaatcg gccacgtgga aatgttggcg
    43261 acaatggttg ctcgtttatt agagggtgcg cctgttgaag atcaagaagc agcgatgcaa
    43321 aaagatccaa caatcggtgc cattatctct ggaatgaatc cacagcacgc gatcgtagct
    43381 ggcttaggac ctcgtcctgc tgacagcgtg ggcaatccat ggagcggtgc ctatatcatt
    43441 gcgagtggca acttgttagc agactttaga gctaatttaa atgccgaatc acaaggaaga
    43501 cttcaagtag cacgtattta cgaaatgatc gatgatcgtg gcgtaaaaga tatgctgtct
    43561 gttctccttg caagagatag ctatcatcaa aatctatggg aatgtgcagt gaaagaatta
    43621 gaagaaaaag aaggttcaat cttagtccca agtactttcc cgcgtgacaa agaacgaaaa
    43681 gacattgctt atgacttcta taacttctca gagggagacc aaagtagtga aggttcttgg
    43741 gctagtggca aagcactgga tggcgaaggc gaatatcaat ggttgaaaga acctaagatc
    43801 gaaggcgctg caccaaaatt agaaccagta acaccaaaaa tgtatgggac acctccattc
    43861 aagaaataaa tatctacccg tagaaacacc cgttttttgt tccacacgct ttgtgagcaa
    43921 aactgactaa agtcattaat cataaccacc cttcaaaagg gtggttttct ctagagctat
    43981 aagctcaaat caccggccag cgcctaaaga cgctggcttt catattttat gttcaagcca
    44041 tattgccttt gccagttttg cccacctctt aaaaggttta tgtactactc cttagccctt
    44101 aaaaggatcg tcttactctc gtacactcag cttatctaac gcactatcat gtttttcttg
    44161 ttcttgaatg tactttttga tggtcgcttc attttgtcct acttttgtca catagtatcc
    44221 ttccacccaa aagtgtcgat acccaaattt atattttaga tttgcacaat ctcttgatga
    44281 tctcctgaat attggctcga tattgattat aaattatttt tcttctatac tttggtgtga
    44341 atacaatgtg gtatttacac atctactttg tacgggctaa actatttccc tttattgctc
    44401 cacccgcata gcaagtggtt ttgtatttca atcgctcggc gcgctcaatt aggtctaaag
    44461 acaataaatc taaaaacccg ctgcttcact gacagaaaca acgggttttc agaaaaaaat
    44521 gaaatgaaaa agatctctta cggcaatcta ttttattttt ttgatttttt ataaatggaa
    44581 aatgcttcaa gaagtccaag aaataaagcc gtataaaaac aagaacctat gaaatcttta
    44641 tttattagat attcaacaga aatcccgata acactagcca acaatatatt tatcagaagt
    44701 gtaaaataag gatgcttttt atagaataga actagtgttg ttttcataga ctctcctttc
    44761 ttcatatttc caattaatca caaattcacg gcattactag aaaaattaaa ggggcgcaga
    44821 agtggaaaaa tactcttcga ctttccctac aaaccaggcc atcttgtgtt taataactat
    44881 accaaatcta aggtacctct aaatctaaat tccatttgtg gcggtgacct ccaattttta
    44941 actcttctat ctcccatcca gtatacataa ccttgatact tcgaccactt atatatcgaa
    45001 cacacaaata cctatccatt gcaggagtac tacctaactt agttgctact tgcatctaaa
    45061 taacagctga aaagatagga cttactctta gtaaagtatc tgaactatta atttttaaac
    45121 tatcattttt atcctcaact acatgcacat ctgttgcaac ttcactttct gtctcatcca
    45181 gataaatgat taaggaatcg ggaattgcaa tatcttcagc tgacgctgga atagttgagc
    45241 taaattagtc atcatatctt tattataaaa taaccaacag caaaaaatac aatgaaaaac
    45301 aaacttggaa ctaatgtatc attaataaat gttgaaaaat tcagcaactc attactgtat
    45361 gattttagca gcccaaccaa catataccct ataaaagcta ataagatggt taggaatcct
    45421 ttagaatttt tactcattca tagacacttc cttaaataac ttttattata gaaggtattg
    45481 taaataataa tattgacaaa aagtgactca aaataaaaaa aaccttacat tatactggaa
    45541 tgaactgtat tgatagtgta cctaaaagct ctactgacat aagatcgtgt aatcggtaaa
    45601 gtaattttat taattatacg ggatgtaatc aattagagaa taaactaatc ataaaaggta
    45661 cttttacatt cataattgtc gctttgagat tctactttgg ttcactatct gacccaattg
    45721 cacattttcg atcattacaa gtattggtgt cgttagaaca actaaataaa aactagcggt
    45781 agatttttca gaatctatgc taatttctgt atggatgttc cagtgatggc aaatttctct
    45841 tttcattgtt tttcttgtcg catgatatgc ctttttgtgt tattcagtta aactatagaa
    45901 attctttcct agacagatct tgatcccaaa ttttacgttt ttgagcatcg atatagttta
    45961 aaatgacatt actcatatct aaagagttta gattttttag gactaaaact gtcagttgtt
    46021 ctttttcttc tttctgttaa agtattgtcg cgacttttcg tatacgttaa gatatgtcgt
    46081 gaaaaaacaa gttttgtgac acaaaaaaac gaatgacttg aggaaatcac ctcaatcatt
    46141 cgtttaaaat gctatatatt ctttagttta agatacggaa attaatgcca tcgaccacac
    46201 tagtagaatg aacgacattt acttggcctt tttttaattt cgttatgttt agcatataaa
    46261 aatattatag agtttactta caatgcttaa aacatatact gtataatctg atcaatttta
    46321 gccatgtctt gcaatggtaa gttttctatt tttttgagtt ttcgttcctt aaaatcaaga
    46381 gattttaatt gtgtaataat tacttgtcct tttatatcta gctcttcagg taatgagtaa
    46441 cgtgtcggta aattctttat agtggaagta attggacata tgaccgaaaa cattgtttta
    46501 cggttaaatt cataacgaga gacgactagt cctggtcgtc ttttttgaat ttcttttcct
    46561 acggagggat caaaatcaat ccatacaata tcaccttttt taggaatata aattctttct
    46621 tctgacacta taaaatttct ctcccttcag gagttaaatc ttcccattcg tctttctcat
    46681 aatactcggc ctcttcccca ccactaaatg gatcttcaat ttttggaact aacgtaattg
    46741 ttccatcatc agaatacaca acgatatatt cttggttttc tgaaggtttc tttccgttat
    46801 cagaaggtaa agttaccact acagaacttc cttgtaatct agattttgta acaagcatta
    46861 tcatcactcc cttaataaaa gtatactata taacactaag taacgcaaaa ttcccctttg
    46921 taaactatct agaaaattgt tttgtttaaa gtatatctta ttggatataa atagaaataa
    46981 attgtagcca gttcattttt tctttaacta aaaaaataat ccaagaaaca ttgaacctat
    47041 ggatccaaaa gctacccaaa tactatagct aaccccccgc taccttcaat tttgcagata
    47101 aaacgctttg ggagaaaatt tcaaagacaa caggccatcc tatcatgccc gattataaac
    47161 aaaaagctgg ctattccttt tgctctgaca aatttcaatt gggaaagttc acgaaactag
    47221 caggtgaaga agtttcaact gttcggatca aagaatgccc gcttcaaata gaaacattgg
    47281 ttcaacgaat ttcaactaga caagactttg ctattgtaga acgtgagatc gtagctattt
    47341 ttgtagaaga agggatttta catgatccaa ctcatatcaa tgtggataaa tggcaacctc
    47401 ttatctataa attcagagaa tatacaactg ccaaccagtc gttaactttg aattttagat
    47461 tccaagaatt caaagagtca taatgataaa catcgttagg tataaataaa aaagactcgc
    47521 agcacgagta aaaaacaaat agaagctata cattagaaag gtcgactgtg accctataga
    47581 tgcagtatat gcagcttatg agcatttctt ctgttctctt tgtttttggg atctgtgctg
    47641 aagtcttttt cgttttttgc cgctctttct ttgaaaaatc gattatgtta taccttctcc
    47701 attactaatt caggcatctg ctgagtaatg atttttcgcg caaaagaaat atctgttacc
    47761 caaggttcga tttgttcttc agtcaagaca agcggcattc ggtcatggat tggtgcgact
    47821 gtgcggttag gttcagtagt cattatgatt gattctgcca ttttttcatc ctgatggcta
    47881 cgataaaaac cgccaatata cactattttt tccttatttg aaaacataaa tttgtctttt
    47941 tcttcattcc actcattggg aaaacacaac gattttgcaa aaatggttga tgaaaggtct
    48001 ttttttcttc tactgtctct gcacgggcat taatcatcaa ttgtcctttt ttaaatccgg
    48061 taaatcccca cttcatgatt tccggagtga ggatgttttc ttcctctttt cttaacgtca
    48121 caacaagatt tgacggaaaa acttctccag ctgctaattc tgctgcctgc ttcggttgtt
    48181 tcgtctgggc ttctcgataa taattctgaa ggagctctga ctctagatca aaaaaataac
    48241 gaccacacat cctaatccct cccaatcttt ttttctatca tacactgcaa caagaagttc
    48301 gcaccaacaa aacgctttca atttttgtag aactaaaaaa gttgtgacaa gtgacaaact
    48361 ctccagtgaa tggtattctc ctgccaactc ctcggtcaca agtcacaata ttcaacaaac
    48421 atgagaagct gctttttgaa attgttcctt gtgactcgga gtgaaacggc aggcttacga
    48481 cctctgggta aacggtgttc aaaagccagt ttctcggtat taagccaaat ttatgcaaaa
    48541 atgtagaaag ctattttcac aaaatttttc tta
//
LOCUS       DACCKU010000010        47605 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.010, whole genome shotgun sequence.
ACCESSION   DACCKU010000010 DACCKU010000000
VERSION     DACCKU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 47605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.010"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..201)
                     /locus_tag="IVN13_000524"
     CDS             complement(<1..201)
                     /locus_tag="IVN13_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7011041.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFT"
     gene            complement(223..1197)
                     /locus_tag="IVN13_000525"
     CDS             complement(223..1197)
                     /locus_tag="IVN13_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011042.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            1497..2369
                     /locus_tag="IVN13_000526"
     CDS             1497..2369
                     /locus_tag="IVN13_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7011043.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIIIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(2951..5149)
                     /locus_tag="IVN13_000527"
     CDS             complement(2951..5149)
                     /locus_tag="IVN13_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011044.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            5381..6373
                     /locus_tag="IVN13_000528"
     CDS             5381..6373
                     /locus_tag="IVN13_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7011045.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            6433..7854
                     /locus_tag="IVN13_000529"
     CDS             6433..7854
                     /locus_tag="IVN13_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP7011046.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPDKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            7847..9346
                     /locus_tag="IVN13_000530"
     CDS             7847..9346
                     /locus_tag="IVN13_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP7011047.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            9385..10779
                     /gene="uxaC"
                     /locus_tag="IVN13_000531"
     CDS             9385..10779
                     /gene="uxaC"
                     /locus_tag="IVN13_000531"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP7011048.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            10938..11621
                     /locus_tag="IVN13_000532"
     CDS             10938..11621
                     /locus_tag="IVN13_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP7011049.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            11623..12459
                     /locus_tag="IVN13_000533"
     CDS             11623..12459
                     /locus_tag="IVN13_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7011050.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            12470..14224
                     /locus_tag="IVN13_000534"
     CDS             12470..14224
                     /locus_tag="IVN13_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011051.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(14500..14841)
                     /locus_tag="IVN13_000535"
     CDS             complement(14500..14841)
                     /locus_tag="IVN13_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7011052.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(14909..17080)
                     /locus_tag="IVN13_000536"
     CDS             complement(14909..17080)
                     /locus_tag="IVN13_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7011053.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            17291..18148
                     /locus_tag="IVN13_000537"
     CDS             17291..18148
                     /locus_tag="IVN13_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7011054.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(18219..19076)
                     /locus_tag="IVN13_000538"
     CDS             complement(18219..19076)
                     /locus_tag="IVN13_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7011055.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(19061..21763)
                     /locus_tag="IVN13_000539"
     CDS             complement(19061..21763)
                     /locus_tag="IVN13_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7011056.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(21776..23065)
                     /locus_tag="IVN13_000540"
     CDS             complement(21776..23065)
                     /locus_tag="IVN13_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP7011057.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            23215..24261
                     /locus_tag="IVN13_000541"
     CDS             23215..24261
                     /locus_tag="IVN13_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7011058.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            24263..26416
                     /locus_tag="IVN13_000542"
     CDS             24263..26416
                     /locus_tag="IVN13_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP7011059.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(26889..28340)
                     /locus_tag="IVN13_000543"
     CDS             complement(26889..28340)
                     /locus_tag="IVN13_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011060.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(28337..30061)
                     /locus_tag="IVN13_000544"
     CDS             complement(28337..30061)
                     /locus_tag="IVN13_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7011061.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(30054..30668)
                     /locus_tag="IVN13_000545"
     CDS             complement(30054..30668)
                     /locus_tag="IVN13_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP7011062.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(31013..32470)
                     /locus_tag="IVN13_000546"
     CDS             complement(31013..32470)
                     /locus_tag="IVN13_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7011063.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(32511..33431)
                     /locus_tag="IVN13_000547"
     CDS             complement(32511..33431)
                     /locus_tag="IVN13_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7011064.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(33443..34390)
                     /locus_tag="IVN13_000548"
     CDS             complement(33443..34390)
                     /locus_tag="IVN13_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011065.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(34869..35588)
                     /locus_tag="IVN13_000549"
     CDS             complement(34869..35588)
                     /locus_tag="IVN13_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7011066.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(35637..37283)
                     /locus_tag="IVN13_000550"
     CDS             complement(35637..37283)
                     /locus_tag="IVN13_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7011067.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            37462..38052
                     /locus_tag="IVN13_000551"
     CDS             37462..38052
                     /locus_tag="IVN13_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7011068.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            38144..39649
                     /locus_tag="IVN13_000552"
     CDS             38144..39649
                     /locus_tag="IVN13_000552"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP7011069.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(39733..41085)
                     /gene="celB"
                     /locus_tag="IVN13_000553"
     CDS             complement(39733..41085)
                     /gene="celB"
                     /locus_tag="IVN13_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7011070.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(41085..41603)
                     /locus_tag="IVN13_000554"
     CDS             complement(41085..41603)
                     /locus_tag="IVN13_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011071.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(41619..41945)
                     /locus_tag="IVN13_000555"
     CDS             complement(41619..41945)
                     /locus_tag="IVN13_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP7011072.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(41958..43937)
                     /locus_tag="IVN13_000556"
     CDS             complement(41958..43937)
                     /locus_tag="IVN13_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7011073.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(43958..44272)
                     /locus_tag="IVN13_000557"
     CDS             complement(43958..44272)
                     /locus_tag="IVN13_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP7011074.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            44440..44733
                     /locus_tag="IVN13_000558"
     CDS             44440..44733
                     /locus_tag="IVN13_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP7011075.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(44811..45923)
                     /locus_tag="IVN13_000559"
     CDS             complement(44811..45923)
                     /locus_tag="IVN13_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7011076.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            46076..46300
                     /locus_tag="IVN13_000560"
     CDS             46076..46300
                     /locus_tag="IVN13_000560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011077.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            46310..46489
                     /locus_tag="IVN13_000561"
     CDS             46310..46489
                     /locus_tag="IVN13_000561"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011078.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            46683..46853
                     /locus_tag="IVN13_000562"
     CDS             46683..46853
                     /locus_tag="IVN13_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7011079.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(47277..>47605)
                     /locus_tag="IVN13_000563"
     CDS             complement(47277..>47605)
                     /locus_tag="IVN13_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7011080.1"
                     /translation="TGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQE
                     VAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKF
                     RKKRNI"
BASE COUNT        14330 a         9568 c         8451 g        15256 t
ORIGIN      
        1 tgtgaagtac gttttcgtca aaggaaacgt ttcttttgag cgcagccatt ttctaatcac
       61 tgcaaccatc gcatacgatg caggaccgaa agtaaaaact cccaaacctg cgatgactaa
      121 accgccccac agaaaattca aataaaccaa ctgtaaaata cgatttagcc ttccgtttat
      181 tttatctaat gactgcagca tatctttccc tctctttcta tcttagtaaa cgccgtcttc
      241 tcctagcttt ttggcaatct tattagctaa aattaccata aacaatccta cgaccccttt
      301 aaacaatccc attgctgtac tgaaacttaa ctgtccattc ttcaatccag ctgtataaat
      361 ataggtgtcg aaaatttctg caacgcttcg attcaatgaa ttcaataaca agtacatatg
      421 ctcgaaccct aagtctaatg tctgaccgat ttttagaatc agcaaggtaa tgattactgg
      481 acgaattgcc ggcaatgtaa catgccatgt tttgcgtaag cgtcctgctc catccatttc
      541 tgcagcttca tacaactgtg tatcgactgc agttatcgct gaaagataga tgatcgtaga
      601 ccagcccaac tctttccaga tcacttgtcc gatatatact gtgcgtgtcc attcagggga
      661 tgttagaaaa ctgatttttt caaatcccat tgttgcaagg aattcattca atacccctcc
      721 gtcaacattc aaaaatacat aagtaatcga aacaatgatt acccaagaca tgaagtgagg
      781 gatgtaaatg atcgtttgaa tcccgttttt caatcgtttg ttcgttactt catttaatag
      841 taaagacaaa atgattggca tcggaaaaaa cacgaagata tttaatccga acaaaatcaa
      901 cgtatttctt aataatgtaa agaatgtcgg ttctgtaaac aaacgaatga aatgtttgaa
      961 accgacccaa ggacttccca ttattcctaa aaatggttga taatcttgaa aagcaataac
     1021 gagtcccccc ataggaagat acctaaaaat caagaaataa aggactcccg gcagaatcat
     1081 cacatataaa aatttattcg ttttgattcc tgcaattatt ttttttcgtc ttctgagctt
     1141 ttcttctttt ttcgatagcg tcagttgaaa tggttcttgt ttctttacac tttccataag
     1201 cccctcctta gttttgtgaa tgtttgctgc ttatgacttc atgatagata actatctgaa
     1261 ttacgtatat aatcattttt atcgtatgta aaaacgctta catagaaatg atttttgtgc
     1321 tatgcaaaac gataaagtgt attttttcac aaataaacta cgcatagcaa accttctatt
     1381 tttttctatc tgacacttag cataggaaat gattatatac gtaatctttc accgatgata
     1441 aattgaacat atagaaacag aaacatttaa tttattttga ggaggagcct atctatatga
     1501 aaaagaaagt ctctatagga gaaagaagtt ttacactttt caacaatacg tttctaattt
     1561 tgctagcttt gatctgtatc gttccttttt taaatatcat cgctacatct tttgcttcta
     1621 cacaagaagt cgtcgcgaaa aagtttatcc tttttcctac taccttttct ttagacgcgt
     1681 atcgctacat tctttctacg ccgaccattt ttagagcact tgctgtttcg attggtgtta
     1741 caggtgtcgg aacgatcgtc agtatgtgtg caacttcact gatggcctac ggtctttcca
     1801 gaaagtattt gtttggtcga ggtttcgtca actttcttgt tgtattttct atgttgttca
     1861 gcggggggat gattccaacg ttcttagttg ttcgttctct aggactggtc aattcttact
     1921 gggccatgat cctgcctgtg gctgttaatg caatgaatat gatcattatg agaaactttt
     1981 tccaagcctt gccggatagt ttggaagaat cagcaaaaat ggatggttgc acagattttg
     2041 gtgtattttt caaaattatg ctgccattag cgttaccgtc tattgctact atctcgcttt
     2101 tttatgctgt tacttactgg aacacttata tgactgctat tttgtatatc aacgattcat
     2161 ctaaatggcc gatacagatt cttttacgcc aaatcatcat tgtatcaagt gggatgcaag
     2221 cagaaagtag tgccgttgat gtgattccac cagcacaaac agttaaaatg gctgttatcg
     2281 ttattgcaac agtgcctatg ctgattgttt atccttttgt tcagaaatat tttgtcaaag
     2341 gagcactcgt aggttctgtc aaagggtgat tatgtactga acgcaaaatg gtagttgtgt
     2401 cagaagtggt cagcttcaag aaataagaag gcatttacga aaattgcttc ttaaattttt
     2461 gtgaaatttc ggcttatttt tcgaagaatc tactcctgga acaccgtgta tcagaggttg
     2521 tgaaagagac gttcagcctt gagcgatgat aaaaactctc acaaacattg cgacgagtaa
     2581 cccacaggag caataagaag cgattttgaa aaatagcttc ccatatttca caaaattatg
     2641 ccttattgtc gatagcctgt ctcttgaacg ccatttattc gtaaaaaaga actgtgacaa
     2701 gacttttgtc acagctcttt ttgcgtgctt agccttgaaa tttaactatc cataaataca
     2761 aatactaggg ttattctaac ttcttttatt gcttgaatca gtccacaaga aaaacagcct
     2821 cttgattaaa cggcgtttat cttgttgatt ctgcggcaat aaaatcagcc agcagcaaaa
     2881 catgaagtgc tgttttttgc tgtcctcttt tattgcttga atcagatcac aagaaaaaca
     2941 gcctcttgat tcacaaatat ttttttcgat attctcccgg cgtaatcgat tccttctttt
     3001 taaagaaacg aataaaattt tgcggatttc gatattgcaa tctttctgca atctctttta
     3061 cggagaggtt agtctctttc aaccattttt ttgctacctc taaacgatga ttttgaacaa
     3121 aatcaccaaa tttttctcct gtttcctttt taaatacatt acttaaatag ttagggttat
     3181 aatgcaacct atcagcgatc acatctaaag acagatcacg atcatattct gtgtagacga
     3241 tatgcataat attctccgac aaatttttaa attcatgatc agtgattgct tgagagcatt
     3301 ctgtgattgg cagaactaat tgttctaaaa caagtttttc tagttctttg ggatgataat
     3361 tgttcagtgc ttttacataa atttgtttca tgttatcaaa gttttttgaa tctactccca
     3421 ataactgtcc taattgaacg agttcattga tcaagcggac cgaaactact tcccgactca
     3481 aaggattttt attttgtgca aacaatgcat caatcaattc atgtactaaa ttcttcactt
     3541 cctgatttcc agaacgaatg gcttcaaaca atcgattttg ttcttctatc ggatatctaa
     3601 taagtgtttt ttcatgagca gcaggaacga tttcctgata aaaaataatt gaacttggac
     3661 cagtatttac acgataatac agggcttctt ttgcccgatc aaccgattgc ttgctttcat
     3721 tcagcgtttc gaaccggtta ctgatgccta tactgatagt taaattcaag tattctttta
     3781 cacgtttttg aatgagtcgg caatactcca tgactttttt gcttgcttct gcatcatctt
     3841 tatccattcg ataaatagtg gcttgcattt cttcattcaa aacgatcggc agcattcgat
     3901 tttcctttgg cacaatttcc gtgatcatgt tgttgatcgc taaaagtagc aaatcacgct
     3961 ccccaccgtg actatcttct aacagatcga tttgaaccaa agctgtataa tagcagtcat
     4021 tttgagaact ataaccaaat tgctgcaacc gctgatctag ttctcgcctt tctactcgat
     4081 ttctaaataa actaagtaca aatagcgttt ccagctgtgg cttttgggta actaagtgtg
     4141 cacttaacgc ttctttttct cctatgatcc gactgactga ttgagcgacc aaatctagtt
     4201 ctttcccact gaatcctttt gatttaagat tcagacgttc ctgtatttga gaaatcggac
     4261 gggcaaatct gtccgaaaat aagtaggaaa gaagaccaat gagtcctacc agtgttaaag
     4321 acactgcaac tagcccgatt ctcagattgc ggatcattgc gccaattgca ctagagtcaa
     4381 cttctaatct gtaaatccaa ttattataat ctgatttcag tactatatac gttttatctt
     4441 tttctcttat cactttcagc tgattgtaat ctagatccac gtccgttaat acagttggtt
     4501 gctctttatt ttttactgtc tgagtagtaa aaaggttgcc aacttcgttt tcaatagaaa
     4561 gtatgtcttc tccactttca ccaacaactt gttctattgt atgtttctct atcgtagcaa
     4621 ttcccaatgc atatttttct ttttcaaaca ttggcaaaga aatcaccatt tcgattcctt
     4681 tgtttttctt ttgccaaaat aaattatttt tgttatttat gtacttctgt tgatactttt
     4741 caacttcctc ttcggtcagt tgactcaacg atccattgat tactccccat ttaccagcca
     4801 aactgactaa atcataatta ttatcatcca taacaatcgt ttctataaaa tttagttctt
     4861 tcctgatttc agaataagtg gagtaatctg tatatgtcag cggatgcttg aatatgtccg
     4921 agaaattttt ggtcgaacca tacgtagaaa acgaatattc aatcgttcta attttccatt
     4981 caacgttagt tttaacttgc ctgagcaaat tctttttgtc tgttaaataa ctcgattcga
     5041 tcgattcata ggtattctgg taaatgaata aactaaatcc tatgacaagt acggctccta
     5101 ataaaaacat cggtaaaaat attttataaa atactgtact tttcttcatt ttcttcctct
     5161 cctcctttta aaataaaagg ctttcatttt tctgtaaaat tgatactcat ctaattaaat
     5221 gagcattccc cgctttcaag aatagcataa ttaaaaataa aaatccaagt aatttttaac
     5281 gtgtgcaatc ttttcatatc aaagatctat tgacttatcg tagttatcgt aatatgatga
     5341 atgcgttaac aaaaagtgaa aaagtaagga aaatgatgga atggtaacga tcaaagatat
     5401 cgctaaagca gctggcgttt ctcatacgac agtttctcgt gctttgaata acagcagttt
     5461 aataaaaaat gaaacaaaac aaaaaatacg acaactagct aaggaaatga attattcgcc
     5521 aaactatagt gcaaaaggtt tagtgaatca aaaaaaatat ctaatcggac tatttttctc
     5581 aagtttgcat aaaggaactt catcaaattt cctagcagat tccattgcag gcatccattc
     5641 agtattagac acaaattaca gtttatctgt tgaaagtatc gatgttgttg atttaacaga
     5701 aatcaacctg caacgctacg atggactgat cgttatgagt caaagtgatg atgatcagcc
     5761 tttcatcgac tatctcaaag aaaaagaatt tcctttcgtt gttgtcaatc gtcacatcaa
     5821 tgatccagcg gttatcaacg tcactgccga tgatgcggcc ggtgttatgc aggcagtaga
     5881 ttacggtatt ggatgcggac atacgaagat cgcttatatt ggcggaaaga aaaattttcg
     5941 ttcgacaaaa gaacgggaaa aaggggtatt gaaaagtcta aaaaaagcga gcatccctgt
     6001 caatccagcc tattttttaa caggagatta cagcttagaa agcggcctcc aaaacatgga
     6061 atacctatta tcactttctg acctgcctac tctggttttt tgcggcaacg acgacatagc
     6121 gattggtgca ttgagagctg tacatttagc tggcctccgt gttccccaga acatttctct
     6181 gatcgggttc gatgattctc cagtcgtctc ctatctcaat ccgccgctta ccaccgtacg
     6241 taagcctttg aaagaaatgt gtcaagaagg aacaaagctt ttactagatt tgatcgaagg
     6301 caggacgatc gaacaatcta aaattgagct ctccaccttg ttgatccttc gtgagtcgat
     6361 caattacttg taaactacaa gtaattttat ataaaaatgt taacgtgtgc ataacacaga
     6421 aaggaagttt atatgaaaaa actgacaaaa gaattagtga aaaaaaacga agcacctgta
     6481 aaagttcttc agtttggtga aggcaatttt atgcgtggat ttattgattg gcagattcag
     6541 cagctgaata accaacaatt gtttaaaggt aatgttgcaa tcattcaacc tttagaacaa
     6601 ggtcttggaa atatgatgaa ccagcaagat aatttatata cggtcatttt acaaggactc
     6661 agcaatggcg aaatcgttga tacttccgag attatcaccg tagtagaaca aatgatcaat
     6721 ccttatgatg aatgggatgc ttatctatct ctagcagaaa atgacgactt ggcctatatc
     6781 gtctccaata caacagaggc aggaatccaa ttcgtggaga ctgataattt aacagatact
     6841 cccccacaga gcttcccgga caaactgaca gcctttttat accgacgttt caaattaaac
     6901 aaagctggct ttaccatcat tccttgcgaa ctgattgaca gaaacggcga acgtctaaaa
     6961 gaaatcattt tacgctacgc agaaaaatgg gatttagaac ctgcttttgt tgagtggtta
     7021 gaaaaagaaa atgttttctg ttgtagttta gttgatcgca tcgtcccagg ttatcctcgc
     7081 gaagatgcag gtgttttcaa tcaacggcat aactatgaag atcaattgat ggtcaaagca
     7141 gaaccattca tgctttgggt gatcgaaggg cctaaagaat tggagaatca actaccattc
     7201 aaacaggctg gcttgaatgt catcatcaca gatgatatga ctccttatcg tgaacgtaag
     7261 gtgcatttgc ttaatggacc gcatacagcg atggtccctc ttgctttgtt ggcggaattg
     7321 gaaactgtag aagaagtaat gaaagatcct ttattctccg catatgttga tcagttattc
     7381 aatctagaat tgattcctat gctttcttta cctaaagatg aattagccat ttatgctgac
     7441 caaatcaaag aacgtttctt gaatcctttt gcccatcaca aattagaagc gatttcatta
     7501 aatagtgttt caaagttcag cacccgtctg cttccagtgt tcaagaagta tatcgaagaa
     7561 caaaatcagg taccaccgtt aatcactgtt tcactagctg cgctacttct tatgtatcga
     7621 ggcgatcaag taaagccaca tgatgatgaa aagacgataa gcgaatttac tgatgcttgg
     7681 tctgataatg gaacagctat tcctcgatta ttgcaaaacg ctgcgctttg gggagaagat
     7741 ttgtctcaaa taccgaatgt gacagataca gttcaagaaa tagctgcaaa gatcgatcaa
     7801 aaaggtgtcc ggtcagtatt acaagaaatg aaaggaacga tggtccatgc ttgaaaccct
     7861 gcaaccaaca atgaaattaa atcgacgaga cagtgtggtc gtcgcattaa cccctattcc
     7921 aaaacaaaca caactgacga ttgatcagca aacgatcacc actcttgaag atatcccgca
     7981 aggtcataag attgcattgg gtgatttgaa agctggagac aacgtgatca aatatggcta
     8041 tccaatcggt catgtgacga cagacgtaac tgccggtcaa tggctgcaca ctcataatgt
     8101 aaaaaccaat ctttctggtg aattggatta tcgatatgaa aaagatgtcc atccaagtca
     8161 ttaccttttt gagaaccgaa catttcaagg ttacttgcga aaaaatggaa aagtagggat
     8221 tcgtaatgat ttgtttttag tacctaccgt tgggtgtgtg aatggcattg ctgaactgat
     8281 cgtcaaacag ttcaaggaaa aacatccaga tctgggacag tttgaccata ttacgatttt
     8341 aaagcatcct tatggctgtt cgcagcttgg aaaagatcat caaaatactc gagaaatttt
     8401 agcggacgca gtaaatcatc ccaatgccgg aggagtttta gtatttggat taggttgtga
     8461 gaacaataca gttcctgaat tcaaaaaaat tttaggtgaa tatgatgaag aacgagtgaa
     8521 atttctagtg gctcaagatg tttacgatga gatcgaacaa ggtgtggctc tattagaaga
     8581 gcttttaaca gcggcggaaa atgaccagcg catatcagtc ccgctctcta agttgaatgt
     8641 cggtttgaaa tgcggcggtt ctgatggtct atcgggaata actgctaatc ctttacttgg
     8701 tgcattttct gactacttga ttgctcaagg aggcagcacg atcttaacag aagtgccaga
     8761 aatgtttggc gcagaacaag tattgatggc acgagcggaa aatgaagaag tttttgactc
     8821 aatcgttgac ttgatcaatg acttcaaaca gtatttcttg tcttacgggg aaccagttta
     8881 cgaaaaccca tctcctggta ataaagcagg tgggatcact acattagaag ataagtcttt
     8941 aggctgcacg caaaaatcag gaagctctcc tgtcgttgat gtgctacaat atggcgaaaa
     9001 aatcagaaaa ccagggctta gtttattgca agctcccggg aatgatcttg tagctgcctc
     9061 tgctcttgct tcatccgatt gccagttggt cttgtttacc acaggaagag ggactccgtt
     9121 tggctcatat gtcccaacgg tcaaggtatc tacgaataca actctttttg aacgcaaagg
     9181 tcattggatg gattttaatg caggcgtctt attagaacaa ccaatggaag taatcttaga
     9241 atcgtttgtt caaaaaatca tcgctgtagc tagtggagaa gaaacgaaaa atgaacaaaa
     9301 tgacgttcga gaaattgcca tttttaaaaa cggagtaaca ctttgatttt cataaaacag
     9361 tataaaaaga aagaaggaag atttatgttt ttatctgatg attttttatt aaaagacgat
     9421 tgggcgaaaa agctttacca ttcctacgca aaaaacatgc ccattattga ttatcactgt
     9481 cacttagatc caaaagaaat ttacgaaaac aacaatttca gcaatctgac agaagcttgg
     9541 ttaggaggcg atcattacaa atggcgcttg atgcgagctt gcggcgtgcc tgaagaaaaa
     9601 atcacaggaa atgcttctga ttttgaaaaa tttttagctt ggtgtcaaac cgtaccaaaa
     9661 attatcggaa atccgcttta cagttggtca catcttgaat tgaagcgttt cttcggtatt
     9721 gacttgttga taaatgaaga aaatgcagag aaaatttgga cacaagcaaa tgaaattttg
     9781 gcagctcctg attttcgccg tcgggaaatc gcaaaaaaat cgaatgtaga agtgatttgt
     9841 acaacagatg atcccgcaga cgacttgcac tatcatcagt tgcttgctca tgaagaacca
     9901 gatttgaagg ttctaccttc attccgtcca gataaagcac tcaatattga aaaagaagga
     9961 tttaatgcat gggtcacact actggaagaa gcagctgacc aaacgatcac cacctatcaa
    10021 gaactgatag aagtcttagg gcagcggata gaatacttcc atcaacatgg atgtcgtgtt
    10081 tccgaccacg ctttagacat cttacgctat aaagaagcgg atgaaacagt attagaagag
    10141 atttttcaaa aagcacgagc taacaaacta ttatcttcag atgaaatcga tgcttatcgt
    10201 acagaaacac tgatacattt gacccacttc tatcattctc ataattggac gatgcagctg
    10261 cacatccatg cttatcgtaa ctgtaataca caaatgttgg aaaaattagg gcccgatacc
    10321 ggctatgatg gaatgaatga tctttctttg actattcctc tgcaaaaatt attgaatcgt
    10381 gcagaagaaa cggatcagtt gccaaagacg atcctttatt cattgaatcc gaatgactat
    10441 cctgcgattt tagctttgat gggctgtttc caaaaagaac agaatggaaa actgcagtta
    10501 ggttctggat ggtggtacaa cgatactcga gctggtatgc gcgatcaatt gacacaattt
    10561 gctgatggta gtgctttggg gaattttgtc ggcatgctga cagattcaag aagtttcctg
    10621 tcctacacta gacatgaata tttccgtcgt gttctttgtg aatttttagg cgaaatggtc
    10681 atgcgaggag aagcacctga agatgaacaa cttttaggaa gtctagttca agcgattagt
    10741 tatacaaatg ctaaaaatta ctttggcttt tttgcctaat tgattaatag taacagtaac
    10801 gaatcaaaaa caggatagtg aaataacgga atccgtttat ccacgtttta gagaagaaga
    10861 cggaccaaat ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt
    10921 atgaaagaag agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa
    10981 aagatgaaaa caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac
    11041 ccgagatcca gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg
    11101 aaggaaggtt agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat
    11161 ttggacataa cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt
    11221 taaaaaatct gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac
    11281 tgacatctgt gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg
    11341 gagactatcc aaaagcaatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg
    11401 tgttctcaag atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct
    11461 acctgcatct tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc
    11521 actttagtcc agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat
    11581 cccgtctgcc tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta
    11641 ggcatccgtg cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca
    11701 cagcttcact tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa
    11761 ttaactgaca tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat
    11821 atcgaacttt ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt
    11881 gcacaagcat tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag
    11941 atgattgcta cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa
    12001 gaagcttatc agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt
    12061 ggcgtaactg ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct
    12121 aaacgtttaa tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat
    12181 ttgatgcata aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat
    12241 ttacatggtt atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc
    12301 cgcatggtac ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa
    12361 tatgagcgtc ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa
    12421 gcaatagaac acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg
    12481 aatacagaaa agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta
    12541 tcagggaaat tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc
    12601 atcttggtta atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc
    12661 ttctcagttg caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa
    12721 tatattgacg aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc
    12781 tggactaaga gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga
    12841 attgcaatct ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt
    12901 gtcctctcaa gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg
    12961 tatcacgctt tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc
    13021 cgcattcgtt actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca
    13081 agaaatggaa gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg
    13141 agctcatggg ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta
    13201 tgaaaaagga ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag
    13261 ttttcagata tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg
    13321 catccaaccc ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt
    13381 ttctggaatg atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat
    13441 tggcgaactg ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca
    13501 tttggatgga aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc
    13561 gattatcgat catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc
    13621 atctggctct ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc
    13681 aggaaaaatt tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa
    13741 ttatttagcc gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat
    13801 cacttatggc ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca
    13861 actttcttca ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg
    13921 caacaagctg tctggtggcc aaaagcaacg tatctccatt gcacgagcgt tgatacgtca
    13981 gccaaaaatc attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa
    14041 aatccagcaa gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg
    14101 gctttcaacg atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaattgttga
    14161 aataggtact tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga
    14221 atgacaaaaa ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg
    14281 gtgttctcct gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt
    14341 tttttgaaat tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa
    14401 tggtgttcaa gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga
    14461 acgtctcttt cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg
    14521 aactggtgtc atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt
    14581 ttgcatcaag tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt
    14641 cattgcatct tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg
    14701 catcgcttgg aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta
    14761 cgcttctttc aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc
    14821 gctatcgtag atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt
    14881 aacatggggt tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt
    14941 tagccgcttc accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc
    15001 cttcttttgc ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat
    15061 tagaatttcc tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg
    15121 catctgctac actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt
    15181 tgaagacttt cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt
    15241 cttcgtaacc catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt
    15301 catttgcttt tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat
    15361 aacctgcagg attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg
    15421 tcatttcatc ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga
    15481 cgatcaccgc tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac
    15541 catcattggc aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca
    15601 gtccaccaag ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt
    15661 ctgctttatc atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat
    15721 tcaaactttg tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat
    15781 tatgcgcatc gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag
    15841 cttccagtgc tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca
    15901 tctgtgtagc aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt
    15961 gtacaccacc tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg
    16021 aagcatttgc tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat
    16081 cctgatctaa agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac
    16141 catactcgtt aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag
    16201 aatctgaatt atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt
    16261 gttcttgttc ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc
    16321 gatcattcgc attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc
    16381 ctgccaaagt agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc
    16441 tggaagcgtc ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg
    16501 tcaagatttc ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg
    16561 cttttcgatc aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga
    16621 tcgtacttcc tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg
    16681 caatcccttt gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta
    16741 ctgcatcttg gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt
    16801 acagtgtgcc agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg
    16861 attttaacgt ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta
    16921 agacacagat caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc
    16981 gctttcgcca tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca
    17041 aagcagcttt tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat
    17101 ttgattcaat cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta
    17161 gttcaatatg tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg
    17221 taagtttttt cttaaatatg ttttaaaaat ggtacaatta gtctgcgatt gcactgaaag
    17281 gagtacactt gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg
    17341 cgacttgctt gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg
    17401 taaaaacgtt tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga
    17461 ccagatcaca ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga
    17521 tgcgaagcac atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc
    17581 aggaatgaag actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc
    17641 tgcttatctc gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac
    17701 aagcggtgcc gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt
    17761 ggaacaaaaa atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga
    17821 tatgaccatc accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga
    17881 tgataaaaat ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca
    17941 aacacaagtc tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc
    18001 tcataaggga catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact
    18061 gatgctccat gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg
    18121 taaagcttta cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat
    18181 aatgataaga ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct
    18241 gctaacagca aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat
    18301 tcacttactt gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt
    18361 ttttgaagga gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct
    18421 aacgtcaacg tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca
    18481 ggatgatctt gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct
    18541 gctgctaatc cttctaaaca atctttatga taaggacaag taccttcata atggtcatct
    18601 ggatggcgtt tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct
    18661 tcattgatca cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt
    18721 tttccttgtg ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc
    18781 caagcagcag gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac
    18841 ccttttttgg gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc
    18901 gggccgaatg agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc
    18961 tgatccaatg tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca
    19021 ccatctgaaa ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt
    19081 tttcctcctc gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt
    19141 gcttcgttgg ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt
    19201 ccaatttttc taaatgacta gacatattga aacctcttac tactacttta tcactaaact
    19261 tgctgcgttt caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt
    19321 ttgaaatcaa tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg
    19381 cgtatgcatg caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat
    19441 tgaaccgatg ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta
    19501 gttctcccac actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt
    19561 cattcaaacc aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg
    19621 cttgctgatg ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct
    19681 tcaccacttc aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca
    19741 agacacgtaa gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt
    19801 tactatcttt tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc
    19861 gttctgcttt tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt
    19921 ctgcagaaac agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat
    19981 ctactaatac ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga
    20041 aaggttgttt aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat
    20101 tattcaactt tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg
    20161 tcaaatgtag agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt
    20221 cttctgcttt cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt
    20281 tttctaatgg taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt
    20341 tcggcaaatc ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc
    20401 cactttttgt ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt
    20461 gttcaggtat tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac
    20521 cagttttatc cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc
    20581 cggtcgtatc gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa
    20641 cttcatctgc tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac
    20701 cgcaaatact gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat
    20761 catggggata gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt
    20821 ctagctgtcg ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat
    20881 tggctaaagt ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta
    20941 agtcttctgg cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa
    21001 actcgtaatc tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta
    21061 ccggttgatg atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg
    21121 catccgctaa tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat
    21181 tcccgttgct ataccaattg gcaaatagca gaccaaagat tgctgtttga tcaggtcctt
    21241 tccaccacat ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa
    21301 agccaattgg ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta
    21361 attgaggcgt ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg
    21421 ttccccattt acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc
    21481 tgataagaaa atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga
    21541 ttgcttgttg gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa
    21601 tcgtttgccc atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta
    21661 gatcgtctat caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac
    21721 gatcccagtg gctatgagaa atgatatata ctttcttttt catttacgat acctcctact
    21781 tttctactct aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag
    21841 aaaaccattc tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt
    21901 gtgttcctgc gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg
    21961 acgttgtcat tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg
    22021 aggatccaat tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca
    22081 atgttcttct tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg
    22141 cggcgatcag attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta
    22201 cttcatatgc ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc
    22261 cctcttggat ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta
    22321 tcgtttgaaa aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta
    22381 ccagatatcc ataacggcaa gtatcatcgc taggacggaa accagaccat gtcattcctg
    22441 taaaagctgt ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt
    22501 ctcgtacaaa tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga
    22561 tttttttgat accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt
    22621 aaagaagata ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc
    22681 agatccaatc gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt
    22741 tgaaggcatt cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc
    22801 tgatcatgtc agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg
    22861 cagtagagtc tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt
    22921 cattttgctt tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg
    22981 cccagtccgc atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg
    23041 attttggtat atctttataa gccactaata aaaccttctt tctatttgat atatctttaa
    23101 agattatact tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact
    23161 ttttcatcca ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgaat
    23221 caaccattgt accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta
    23281 cctgtagaca gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga
    23341 atcacttcca aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc
    23401 gggaaaggca gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa
    23461 acacgacgga ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc
    23521 aagggattga accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac
    23581 tttctacaaa ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat
    23641 gctcttgata cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct
    23701 gtcgtcatat tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat
    23761 ttccaaggcg gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct
    23821 tatttgtttg gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt
    23881 gaagcattgg acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca
    23941 ttagatgaat tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac
    24001 gatgttgtcg ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa
    24061 gatttttcaa ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg
    24121 acaaccatcg cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact
    24181 tggttagaga caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga
    24241 caatcaacaa aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc
    24301 gaatcagcca aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat
    24361 gaattatttt gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga
    24421 ccgcgttttt caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag
    24481 tcatatgctg tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca
    24541 aagttcttac cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt
    24601 acgttatggc atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga
    24661 ttacagcaaa aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt
    24721 tatagatcct tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga
    24781 tttttctttt tattttatat tgagatttga atatccttta acaattgatg agttagctgt
    24841 ttccaacaaa aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat
    24901 ccgatttggg acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca
    24961 aaaccaaagc aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga
    25021 tcgtatacag ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata
    25081 tcgggtgttt ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca
    25141 ctatgatacg actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg
    25201 ggatacgtat aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga
    25261 aatgctggaa ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc
    25321 tcctgatgaa cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc
    25381 tgtaaaagat atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc
    25441 tacggttcaa agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata
    25501 tggctatgtt cctaatcact accacgaatc agtcaatcac acattagact atgcttatag
    25561 cgatttttgt attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata
    25621 tcagcagcaa tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc
    25681 aaaagataaa gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta
    25741 tgcagaagga agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat
    25801 cggaaaatat ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc
    25861 cccaacgttt gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc
    25921 gatagaattt ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt
    25981 cagttatata ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac
    26041 cttcgacgat tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg
    26101 gtatatcttc aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat
    26161 cggtatgcca ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat
    26221 tgaagcaacg cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacaa
    26281 agaatatacg aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta
    26341 ccaattaggg atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt
    26401 atcaaactat aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca
    26461 gccaagcaaa aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa
    26521 gcttttgaac accgtttatt gagaggttgt gaaatcagcg ttctgacctg agtattagag
    26581 agacaaatgg caaaatagtt cctcatattt tgccattttt tgagctaata ccgagggtca
    26641 gctgattgaa caccgtttag tggagaggtc gtgaaaaagc tgtttagttt cgagtattaa
    26701 gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta ataccgagaa
    26761 actggctttt gaactccgtt tatcgagagg tcatgaaaaa gctgccttgc ctcaagtaat
    26821 aagaacagcc caaaattaga tttctagaca attttttata agttatttca ttacattggc
    26881 tttttctttt atgattctcc actgttttct cggtactctt taggggatct gccgacgatt
    26941 ttcttgaata atttagagaa atacgtggtg ttgttgtagc caatctcact tgctatctca
    27001 ttgatgtttt gattactgtg caaaagcagt tgtttcgctt tttccatacg taaatggttc
    27061 aaataagctg aaaaactttt tttcgtttct tttttgaaca gctgtcctag atacatgaca
    27121 tttaaatgca ggttttctga cacttctttg agtgtaagtg gttcttgata atgctggtgc
    27181 aggattacga ttactttttc cacattgtca ctgtactttt tctgtctctg atgctttttg
    27241 atgaatgaca gaagtagctg ctgaagttct tgcaccgttt ttgcctgatg gattttttcg
    27301 attccttgta aatattcctc atcttccaga tcaatcaatt cccgttgaat ctccataaac
    27361 agtaaaaaac tggaatatcg tatatcttcc ggcatcatgg ctgctaactg aaactgttca
    27421 aaaaaatcag tgatcatttt ttgagcgact tccaattgtt tattttgcag cacgcgtctg
    27481 aaacttgaaa aatcgatggt ttgatctttt gtttctgctt gtactgcgta aaaaacatgc
    27541 tcgtctttat ctccataaaa ttgatgcaac tgtagattgt ctttcgcttg ttggaatgtt
    27601 tcagggattt tgtccaaaga atctgtctct tccccgatac tgatcagcca ttcctgatca
    27661 ggaaagtttt cttgaacaaa acaacagaag ccatcaactt catctgcctc caacaacaac
    27721 gtgatcaata cacggtctcc gtaatttcgt tgatagtaaa agatttgttg ccgttcttgc
    27781 aaccatctat tgataagagt atcatgagtt cttgccagtt gaatcaatac tatcctatgc
    27841 tgcttctcac ttatttgtcc tattatttct tcttcactta tttcatccaa ttcttcattc
    27901 aaccattgac tgaataacag ttcttgatag atttcttgtt gatttttttg ttcattttgc
    27961 tgatctaacc gtgttttgat tttcttgagg ctttcaacca attcgaactt attcacgggt
    28021 ttcatcaaat aattcacggc gcctaattgc atacctcctt tcaaataatc gaattcttgg
    28081 tatcctgata aaatcataaa ttcaaaatta tgacgttctt tttgagccgc ttcaataaat
    28141 tccagtccat ttatttctgg cattgtcaca tccgttacta ctaaatcaat ctgtcgctgt
    28201 tcaagtacag ctagtccttc tttggcattc tctgctgttg ctactatttg aaagcctaaa
    28261 ctttgccaat caattatctt ttttaaccca ttcaaaatca tgtattcatc atctaccaat
    28321 aacacttgat acacgctcat ccctccttga atgtaataga aacggtcagt ccacctgtct
    28381 cattattctc aagcgacata taataactgc tgccgaagct ggcacgtaaa cgttcattca
    28441 cgttttgcag accgatagaa ccgtgaagtt ccactttagg atgagacagt ttttcttcaa
    28501 tctgttttag ccgtttttca gtcatccctt ttccattatc tttgatgatg atccttaccc
    28561 gtttgccttc ctgcaagact ttgacactta gcgcattatc gaatcgagta aaatcaatac
    28621 cgtgtttaaa ataattttca actaatggct ggatcacaaa tttaggtact tcgatctttt
    28681 ccaaatcagg atcgatcata aaatgatagg ctactcgatt aggataacgc atttgataca
    28741 aatagacata tttttcgcaa aagtccagtt cttcttttaa agtgatcgtt ttctcttgat
    28801 tcgtattgtt tcgaagcaaa gcggaaaacg catacacaac atctgctaat tcttcacttc
    28861 cttcgctgat tgcatacatt cggatatatt ctaatgtatt atataaaaag tgtggattga
    28921 tctgtgcttg aagcgcccgc atatgtgcgt cttgctgttt gatttctaat ttgtagatgt
    28981 cttctacaaa ctgattgatg ctatccaaca tttcgttgat accgattgaa agttccttca
    29041 gttcatattc cgtgtttgtt atgtcgatcc gcgtctctaa attccctgct gctgtttgat
    29101 ccatggcctg catgacgata gatacttgct gtgaatatcg tttgaatgtt ttataaagaa
    29161 agataagcag tatattgtct aaaataatga taccaatgat cagcggggca agattttttc
    29221 ccgtttcgat ttttacactt tttttatcta gtacagctaa tatacgataa tcatcagaca
    29281 gttcttggtg ttgaacaaaa ttgttttttt ccagatcgtc aatcggcaat tgtgaatttt
    29341 tcttcagact ctctgtgatg atcatttctt gtctttcaaa cgtttcttta gctattttat
    29401 tatcatggaa tgtgtatagt cgatttgtcg ttccagaaat catgtataaa gagagtccat
    29461 tgaatgacgt tagattttct aaaatcatat ctagttcttt cttttgaaaa ccaataaaaa
    29521 tacttcctga ctcagctcct ccttgtgtga taggcaactt gatataaaag gcgtctagta
    29581 attttggtgt cccctttttt atttccccac ctttgttttc tctagaagat tcataatact
    29641 ccggtagttg attcaacacg atatatatcg cacttagttc ttcataattt gcgtagagat
    29701 ctttgatttg atttgggaaa gaaactaatt catcggtact gttttgctgt tcgtatacat
    29761 acatcagata ctgatctatg ggttttgtta agtaggtcgt cacattttcg atcttttcgg
    29821 aattccctac aagctggttt ttgatgatcc tactcaaggt tgttttatca ttcagcattc
    29881 tgcttgtgct ttgaacggca tcacttgtca tgttttctgc ctgtttttga ttttgttccc
    29941 aagtatggat actaaaaata acggtagcag tcatcgttac accgatcatg ataaccgcgt
    30001 atttccgcaa cagacgattt agtaaggttc cttttgaaaa tagaatccga aacttattca
    30061 taactttcca actgtttatc taattcttca cgccaatgtg ttgtcatcgt accattccaa
    30121 accgtcaaaa gaccaatgac accaaacagg atcaatcctt tgaattgcca agtcacgcct
    30181 aaaattatac cgctaccaat aattattttt agaaaagacg aaaagctgga aaaaaccgaa
    30241 ataaaactta gctttagcag attaggaaca gagatcgtat attcactatc taaaatcata
    30301 ctgtattgat aagtagcaaa taggcagagc aaggcaaaaa gtaaaataaa atcaatagct
    30361 aaaaaaatga gccctttgat ttgaacagat aaatataaat tgtaactcaa taaagcagta
    30421 ccagataaaa ataaactaaa tagcagattg cctcttaaaa aatttctttt atatatattc
    30481 cagcctcttt ttattgtgat ttctttatac tcgaatccgt acaaataaaa tagttcattg
    30541 actgttttcc atgccgcacc taatccaaag atgatcccgc cggcaaatgt atatatccag
    30601 aaatacaagg ttagttttat aatgacccag atccggataa ataactcttc caaaaccttc
    30661 cctaacatat cttctcctcc aaacattaag tttatacttt ctcattatac aaaaaaaggt
    30721 tgggcaatca gcaccttgac ctgattatta gagaaacaaa cggaaaaata gttcctcata
    30781 ttttgtcgtt ttgtgagcga ataccgaggg ttagctgatt gaacaccgtt taccaagagt
    30841 ttgtgaaata gccgcttagt ttcgagtagt aagaaaaatt ttgtgaatat ggctttccac
    30901 atttttacat atatttagct taataccgag aaactggctt ttgaacaccg tttattcgga
    30961 acaagaggtt gtgacacgtc ttttgtcaca acccctttgt ctttattcat tattattctt
    31021 gtcggaactc atcatattgt ttttgcattt ctttcaatac cttatcatag ccagccttgt
    31081 ccaaagcgtc tttcaatttc ggtaatgttt catctggatc aacagtccct gtattcaatc
    31141 catctaaata ttgattcatg acattagaaa tattacttaa ttctgttttg acactgtcag
    31201 tgttgaagct gaatcctaaa atcggtgaag tttctgcatc agcaatcgat tgatcacgtt
    31261 tggcaatcat gtcatccgta atagactctt gtgtatatag aatcttgttg tttcctgtat
    31321 tccaagcaga catatgtgta tttggctgat aaccatctag caatttgatt ttatcatctg
    31381 aaccatcgac tttttcccat gcttctccct cgatccccca aacaagccca ttcaataatt
    31441 ctggatcact gttaagcagc cctagaagtt ctactgattt ttctttattt tttgaagtat
    31501 ttgatacgac aaaatttgcc atttgtgctt gagctgtaga ttttagtgga acagtaattg
    31561 cttttgagac aagttcttgt tttgctgcat ttgtcaaaat tgtatcgcca taatcatacg
    31621 gtccttgtgt ttctccacgc attagccaag tatttccttc tagtggataa tccttattgc
    31681 tcgttgcagc gtcacttgga atgagtcctt gtttgtacca gctatgcatt gtacgtaaca
    31741 gtccgatata cgtttcatta tcgtattggt tgatgatttt cgaatcatct ccatccagat
    31801 caatagcaaa tggtaacgta ttccctaaag gataatcaaa gttcccttgg actttgaagc
    31861 cttgtccaat ggcaaaagct gccgtgtttg gttctttttc atggaattcc ttcaagactt
    31921 tttctgcatc agcgtaagat tggattccat caattgaaag atcgtattta tctagtaaag
    31981 ctttgttaaa agtaagtact tgttgagcaa agacatttcc attgactggg aaagcatata
    32041 attttccatt gaccagattt cctttaatat atgcttcatc taaatcatta taagcttttt
    32101 cagcgtactt aggcgctaat tcagtcaaat cagcaaaggc tcctttttgg gcattggaaa
    32161 catagttgtt cgcaaaggcg atatcataat tttccccgga agaaatgatg acgttcattt
    32221 tcttttcgta atcgccccag ccaatgtaat tgatattgac ttttacaccg atctcttttt
    32281 cgattcgttt gtttgctact tccatcaatt gatcgaaatt atctggttta tctccgattt
    32341 gatacatctg caaagtgtcc gaattgctag cttctgcctg gtctttagaa ccagaacctc
    32401 cgcatgctgc taatgttccc aacaacaacg ctgaaacacc tgtaaaggct agtttcttcc
    32461 ataacttcat ttttcttcct cctaatttta tgatttatat ttttgtttct ttattctttt
    32521 actccgccga ttgttagacc tttgacaaaa tgcttctgga aaaatggata gctgatcgca
    32581 ataggcaacg tcgagatgac tacgatcgcc attctggcag attctcctgg aactgaggct
    32641 aatccgcctg tcaattggct tcctgctcct gaattttgtg tcagatattg aatattactt
    32701 tggattttca tcaaaagata ctgtagtgga actagcttat catcttggat gtatagtaaa
    32761 gcattgaacc agtcattcca gtaacccaat gcggcaaata aactgattgt cgcaatacct
    32821 ggaacagcta acggtaaaac gatctgaaca aatattcgca gttcgctagc tccatcgatc
    32881 cgtgccgatt caataatcga atcagagacc gaacgtttaa agaatgtgcg catcacaata
    32941 atattgaagg gactgacagc cattggcaag atcaatgccc aaatcgtatc ttttaactga
    33001 agaagatttg tcataacaag ataattggcc accatccctg gtgagaacaa catcgtaatc
    33061 aaacaaaaaa cggtaaaaaa acgacggaat ggaaagtttg accgtgaaat cgcataagca
    33121 tataaagaag tagccgtgct attgatcagt gtacctaaaa cagtaacaag tacagtgacg
    33181 aaaagcgctt ggaaaatttt atcctgcatc tggtcgaaca agtacgtata accaaatata
    33241 ctgaactttt ctggccaaaa actgtacccg tgagtcgaca atatggactc atcggtaaaa
    33301 gagatggcga taacaaatat aaatggcaaa atacaggaaa tagcaaagag tgcaatcatt
    33361 atattagaga aaaaattagc cgtcttattg aaagagcgga cttggactct ctccactttc
    33421 ttttttttca tttttttcct ccttagaata atgcagaatc gctatcaaaa cgtcgggcga
    33481 tcgtatttgt gatcatcaac aagacaaagc cgacaactga ttggtaaagt cctgcagcag
    33541 ccgtcatacc aaaatctcct gtggatgtca ggccattgta gatatacgta tccaaaactt
    33601 gagtcacatc atataatgca ccggaattcc taggaatgtt ataaaacagg ccaaaatcag
    33661 ctcggaaaat gtttcctacg gctaaaatcg ttaaaatcgt cattagtgga ataagttgtg
    33721 gtatcgtcac atgtttgatc tgctgccatt tgctagctcc atcaaccata gcagcttcat
    33781 aataagtcgg atcgattccc ataacagatg caaagtaaat aatgctgtta taaccaatcc
    33841 ctttccatgt accaatgaac accaagataa acggccaata tttcggttca ttataccaat
    33901 tgacagcttc tcctccatgt ccagtgatcc actgattgaa tattcctttg tcaggactta
    33961 aaaaagcgta aacaaagtaa ctgataatga cccaagatag aaaataaggt agtaaggaca
    34021 tcgtttgata aactttaacg agtcgtttat ttctcaactc gctcataatg attgcaaata
    34081 cgactgagat cagcagattc aaaaggataa atgctacatt atataaaatt gtatttttag
    34141 tgatgagaaa tgcttgatcc gaagaaaata aaaatttgaa attttcccaa cccacccaag
    34201 gactttccct caagctagcc aagaacccat ctgctgaaat atgaaaattt ttgaatgcta
    34261 cgacattcgc cattacaggt atgtaaaaga aaaaaatcat ccaaagcata cccggtacag
    34321 ccatcaggac aagtgcttta taacgccata gattcataaa aaaatgattg aacccctttc
    34381 gttttttcat ctaaaaaccc ctttcgattc attcgcaacg taattatagc gctttcaata
    34441 atgagaataa ataaacaaag tatagggaaa atgaaacaaa gtataggaga ggttgtgaaa
    34501 tcagcgtttt ggcctgagta ttagagaaaa aaacgggaaa atagttcctc atattttgcc
    34561 gttttttgag ctaataccga gggtcagctg attgaacacc gtttatcaga ggttgtgaat
    34621 tcagcgtttt ggcctgagta ttagggagat cgtgtcctta ctatctaact ctgagtcaca
    34681 aggaacaatg gggactgtaa caaaagtctt gtcacagtcc cttattccga acaaacggtg
    34741 ttccagaagt agcttctacg gaaataagcc gaaatttcac aaaaaagcca tccgaaagtc
    34801 agacggacaa gtctgctttt cagatggcgc tacaactact ctgcttattc ttttagctat
    34861 ccattcaatt atttttctct cacttgttct agctcttctt tggtcatggg tcgatattgc
    34921 cctttattca aatctttagg cagccaaaat tttcccatac ggattctttt taaatataag
    34981 acttcttttc ctactgcttt gaccattcgc ttaacttggt gaaactttcc ttcatgcaaa
    35041 atcagacgga taattgaagt ttctgtctct tgatctactt gatcaatttg gagttttgcc
    35101 ggcaatgtct gctcatcgcc atcaataatc agtccttctg caaataaacg gatatcttct
    35161 tctgtcatta taccgctaac ctcagctaga tattctttct ccacatgttt tttaggtgat
    35221 agtaactgat gggcaaacac cccgtcattc gttaaaatca agaggccctc tgtgtcttta
    35281 tctaatcggc cgactggaaa aagatcttcc cgataatcct ggtcttccaa taaatcgatt
    35341 acggtttgat catgattatc ctgagtagca gaaatgactc ctgcaggttt atgcaaaatg
    35401 tagtaaaaat ctttttgata cgtaatgatt tctcctaaat aagtaaccgc atcttcgaat
    35461 tcatttactt gcgctttatc gcttttcacc acttgtccat tgacttcgat cgttcctttt
    35521 ttgattaatc ctttgacttc tttgcggctg ccaagtccca tatctgctaa aaacttatct
    35581 aaacgcatgc gtttccccca tttcaaaaga gcatcaatct agctcttctg attcacttat
    35641 tttattttta atttatgtcg taaacgcgct attcctggtc ctagtagtct ttctgccaag
    35701 cggattttca aagctaaata accatacact aaagccccta tacctgctac tacaagaatc
    35761 aacaatagag actggaattt gctatcttcg tttaaaaaag caccaaaaat catctttgtg
    35821 atccacgaag ccaaaagcat aatcaaggta agaataaaga tcagcaatgt cctgcgccag
    35881 acaaaacttg gtttaaagcg ggctacttca tggattctct ttaagatcaa ggtacaagac
    35941 acagcaaatc cgatcattgt cgccaataaa gggccgtata cttcaaataa acgaatggct
    36001 gggtattgaa gaaggagttt cactagaaac ccaataacca aataattaat tgccgccttg
    36061 ttttcaaaca ttccttgcag catgttggaa actagcatat atagtcctag gaagagtcct
    36121 acaaaacttg cttggatcag tacattactt cctagagaat ccggtgtata aaataatgta
    36181 tttagcggat aggcaagtaa cataacacca aatgtcgccg gaaacataaa aaatgaaaac
    36241 aactgtaaat ttccgcttgt caattttgcc agacctactc gatctttcaa cgtaacagct
    36301 tctgtaatca acggtaaccc agtagacgcg atcgatgttg ccaatgcaat aactaccatc
    36361 gtcaacttat ctggattggc actaaaaatc gaaaaaagat ccagtaactg tgcattggaa
    36421 tactcggtgg atgcagacat gatcttcata aaagtgaact gatcgactaa tttgaagatc
    36481 gttacccctg aacccacaat aataaacgga atcgcttctt taacggtatc taacaacaat
    36541 tccttcgttg caatatttat tttgttttcg ctgtagtgaa taaacgccga tgtatacgct
    36601 tgatgtttct tcaaaaaata caaaaggaca agaatacttg ctagcatacc gataaatgcc
    36661 gcgaaagttg attgcgtgac cgccgtcaca tagtcaccat ccatgacttt catgatgatg
    36721 aatgtagcca atagcatata aaaaatacgt gccacctgtt ctacaatctg cgacagcgca
    36781 taaggcatca tttcttgatt gccttgcaaa taccctcgta tcacactcat acaaggaaaa
    36841 accaatacag cgatactcaa agaacgcatc gttggaatca gttcttctcc gccaccagaa
    36901 gcatgagcta gccaaggaga ggcgatgtac atgaacaaag caaacaacag gcctaacccg
    36961 cccatcatct gtaatgcacg gataaataat cgtcgtgaag tgccatattc attcaatgaa
    37021 ttatagcgtg cagtttgttt tgcaatcgca gcaggtattc ctgctgtaga gatcagtaga
    37081 aacaatgcat atatattgta tcccatattg aataatccat tggcagcctt agcattttcg
    37141 cccatccaag cataccaggg aatgatatag acagcgccta gcaatcttga aatgatatta
    37201 ctggcggtca tccaagcaga gcctcgtgcc attttttctt gcggtgttaa ttgatctaat
    37261 gctggttttc tttgattatc catcggtcag attccaactt tctacaaaac aatactatca
    37321 ttttaattgg taaaacagaa actggcaatc gattttatat aacttttata agaacaattg
    37381 tttttttgaa ttcggtttcg tttttttggt aaagtaaaga aatatacagc cttttatggc
    37441 taacatttat aggagatatc aatgacacaa gaaataaaaa aactacccgt tatattttta
    37501 ggtcttttct ttatcacagt tggattgaat tggttcttgc tccctcacga tatagcttcc
    37561 gccggagtgg gcgccatcgg acatttagtt gaaacggcct tttctatcca acgctcagtt
    37621 actgtttgga gcatcaatct tatgatgctg cttctagccg ctgtcctatt aggaaaatca
    37681 gtatttttta agactgtagc gggaagtctg ctttttcctg ttgttttaga gattgttcca
    37741 aaagtggaaa tgatctcctc acatttcttt tcactgattt caggaagtct tttgttcagt
    37801 cttggggttt acacattgta cacagtcggg gcctcaaatg gcggtataac gataccgccg
    37861 attattttcc acaaattttt tcggcttcca atgccgcaag gacttttact taccaatagt
    37921 cttatcgtct ttttgaatta ttatgttttt ggattgttgg agacactatt cgttctccta
    37981 tctattacag tcagttcatt gtttatgaaa atactcatcc gcttgtcccc tgtgtcaaga
    38041 aaatttgaat aaagctggat tttgtcatag cggaaacaaa cgatatatta aaaagagtgt
    38101 gctataataa accaagaaat tgaataaagc gagggatcaa tcaatgagct tctctttaga
    38161 agacatccgt catttactat taaaagaaaa tctattaaaa gaatttgttt caacaagagg
    38221 ctggcatctt gatgtaccag aaaaagctga atttcttcag ttaagttatg attcaagaaa
    38281 agctgatgct tctactttgt ttttctgtaa aggaatgaat ttcaaagaag aatatttgga
    38341 ttcagcaatc gaacagggta ttcaatatta tgtatcagaa caaccatacg aaaattcagc
    38401 agtaggaatc atcgtaacgg atatccgtaa agcaatggcg cttcttgcta tggcttttta
    38461 cgactatccg caaaataaat tgaaagtgat cggattcact ggaacaaaag gtaaaacaac
    38521 tgctgcgtac tttacaaaag caatcttgga tcacacaaca aataaaaaaa cagcattgct
    38581 ttctactatg aatacaacgt tagatggcaa aacatatttc aaatcccatt tgacaacgcc
    38641 tgagtcatta gatttatatc gtatgatgtc agaagcagtt gaaaatggta tgactcatct
    38701 agtgatggaa gtttcttcac aagcttacaa aacacaacgc gtatacggct tgactttaga
    38761 tgtcggaatc ttcttgaata tctcgcctga ccatatcagt ccgatcgaac atccgacatt
    38821 tgatgactat ttctattgca agcgacaatt gatcttgaat tcaaaaacag ttgttttaaa
    38881 tcatgaaagt gactattttg atttacttag ggaaacagcc gatctattcc agattccaac
    38941 gatcacttat ggtcgttcgg aagaaagcaa ttatcaagtg atccgttcag ataaaggtac
    39001 tcacggtttt actttatctt ctcatgaaga tcgcttagct atctgcgata ctgcttatga
    39061 tattttgctg gctggaggat tcaaccaaga aaatgcagcc agtgcgatca ttgccgcagc
    39121 actaacaggt gcctccagtc aagatgcaca agacggatta aaagaagctc gtgtaccagg
    39181 ccgaatggat caattgatcc aatccaatgg agcgcatgtc tatgtggact atgcacataa
    39241 ttatctaagc ttgaaaacat tgctgaaatt tgccaaaaat gaacatccag atggccgtgt
    39301 aattgttgta ttaggcagcc caggaaacaa agcaatctcc cgtcgacatg attttggaaa
    39361 agtgctatca gaaacagctg acatcgcctt tttgacggca gatgacccag catttgaaga
    39421 tccgaaaaaa atcgcggaag aaatcaatga agcaattact aatccagatt tgatcgttca
    39481 ttatgaaatg gatcgtcctg aagcgattcg acgcgcttta gcagaaagcg ctccgcaaga
    39541 ttcagtcgtt attgcgggaa aaggcgtgga tccctatcaa aaaatcaatg gtgtagatga
    39601 accttatgaa ggggattatg caatcgtgaa gcggttgatt gaagaataat ggtatttata
    39661 tactagtggt ttcccatttc taagaaaaaa agggactgtg attttttgtc acagttcctt
    39721 ttttcttaga aatcatttta acttttcact agctaattct gctgcctttt ctctttcttc
    39781 atttaataat ttattatcat agaatttgat aaatgggaac cagataatga atgcgatgat
    39841 tgcacaaatg actgctaaca cagcagcttt ccaatcacca ccactaccaa caaatgctcc
    39901 gatcccgact ggtgaaggcc acggcatctg tgcaagcatc ggtttgacga tttccagccg
    39961 aatggcaaaa taagcgagtg tagcagaagc catcggagct aagaaaaacg gaaccgctaa
    40021 atagggattg tagacgattg gcatcccgaa aatgattggt tcattgatat tgaataatcc
    40081 tggtacaagt gaggcttttc ctaacacttt cagctgatca gatttcgcga ggaatgcaat
    40141 aaagatgatc aatcctaaag tcgcacctga tccaccaacg gttacataag aattgttgaa
    40201 ttctcctgcc aacggataat ttgcgccttg tgcgttagct gccatattag ctaatacgat
    40261 cggggtcaag aaagaagtga tgatattcgc tccatggatc cctacgatcc acaatgcatg
    40321 gatcaagaaa taaatcacca tgacaccaag ccatgtattc gtcaactgcg taacaaaccc
    40381 aaatgggata gcaatcattt tgaagatatc cgttcctagt gcaactaata atccattgat
    40441 gatcatcacc acaaacgcaa tgacaaatgt tgggatcaga gcagtgaaag aacgagaaac
    40501 tcctggcgga acaacatctg gcatttttat gacccaattt ctcttgacac acatacaata
    40561 caattcgacc gcaataatcg acataataat agctgtaaag attcctgaag ttcctaaacg
    40621 ttcgacaaaa gagcccatcc taataccatt tactacagtt tctgtctcat tgatagaagc
    40681 aatcaagctg attttaccat cagtcatgat caattcaggg atacacataa agaatgcaaa
    40741 aacagatagt aatgcccccg tgagcggatt aacatttaat ttttgttcat ctcgttcaat
    40801 tgacgttaat tcataagcaa acacaatggc aaagtaaata gataaaatcc ccatcgtcat
    40861 tgtattgacg atcatgtaca agtcactaac tttgaaaaaa gttgcattaa aaaatccttc
    40921 taaaaatggg aacggtaatg gcaaaatatt taaaaccaaa aacatcgagc ctacaatcgt
    40981 gaaaggaata gaagccatcc ccgctgccat gacagcccga acgattttaa gttgcgcaat
    41041 tttccccata ggtcccatca ggtatttctg caaaaaatca aacattagtt attctccttt
    41101 cctaaatata acgctttttc atattgttta atacggatat actgttggtc ttggttgagt
    41161 gaaattttct ttaaacgata ctgtataaat gcactggcaa aaataccgct tacaacaaga
    41221 atgaaaacaa ataactgtcc tccattgtta tttgccataa aaggatacaa gaatgtaaat
    41281 cctgaggtaa acaacactgg aatcactaat aaattaaaga cactctggaa caaaaaatac
    41341 cactttgtcc atggaacgtt cgtttggtgt tttcgataaa gctttacttg ttctcctact
    41401 gcaggcaaaa tagctaaaat cagaatcatc ggtaaactac ttcctaaact tttagctgat
    41461 agaagcagaa tcatccattg catgttcatg aaaaagaaaa aggcagtcag gtaacgtacg
    41521 attagataac gattgaaata catgtttttc aaactaagtt gctttttttc taattcgatt
    41581 ttccttttta ctgtatcctg cacaatgatc cttcctttct atttattttt ttgatacaaa
    41641 taattcattt cctgtgcaac ttccaataat gtcatcgtcg tcatcagatg atcttgtgca
    41701 tggaccatga taatttccat ttctattttt gtcccgctgg catattcttt cagaagttga
    41761 gtttgtgact gatgcgcatg aagaatctct tcatttgctt gtcgcaactt ctcttctact
    41821 tcttcaaaca tctctttacg catcgagcga aatgcttcat gtatcagtgt ccgtgcattt
    41881 ccgctatgga aaatgatatc aaaagaagtc acttgtagtg tttcactgtt tattttttca
    41941 gccatctgtt ttcctcctta ttcgatcagt ttgataaatt tactgctgaa attctcaaaa
    42001 ataggttcag ctaagatttc ctgctgtact tttagctggt caactaacct aacgattgct
    42061 ttagtcacga ccgtaattcc ttcattttcg acataagaag gcgaaactaa aaaaacaaaa
    42121 tgaatttctt gatattgatc ccactgcatc ccagaaggaa tcaatgcaac ggctaccttt
    42181 gtttcgattc caactggaac agcgggatgt gggactgcca ctgtatcact gaatatgatt
    42241 tgtcccatat tttcacgttg ctggatctgc tttcgcattt ctcttatata tccttcttct
    42301 tcatttatgc tcagcttttg cagcaaatca tgaagaattt cttccttttt cggttcgtac
    42361 gtataagtct tgaaaaattt ttcagacaac tgttcggtgt aaatctgctt tttttgctgt
    42421 agagaaaatg ctggtatcgg tctatctgag gaatgatagg gtatagattc tccaactacc
    42481 tttcgtattt tacgaacatc atcttcattc aaaaaaatac tgacatgtaa aactggaatt
    42541 ttgaaaaaca ttgtcgaaag atcaatagaa gaaataatca ggtcgattcc ttttaaagtt
    42601 gtttcattga tctcataata gccttttaca cttaccactg taatattttt accgaattca
    42661 ctgactacgc gattctttaa tagttgggca ctgccgtaac ccgttgcaca gataatcaat
    42721 gcatgtagtt tatgcgcagc tctttctttt tccaatgccg ccatgagatg caaagccaaa
    42781 taggcccatt catcttcatt gatggtatag tttttcaaag ccggcatgtt gctgaaatgc
    42841 ctttttactg cgtcaaagac tttgggattt tctttcttta tctcctcagt caatgggttc
    42901 tcaatgcgaa tacctcggct caatcgaatc atcatcggtg acaaatgatc gagtagtcca
    42961 ttgaaaagct ggagatcctc tactaatgaa aaatgaaatt cctgagacaa tcttttcaga
    43021 actttcgcta attcctttga tagttcctga tcattttgat tttctttgtg attcgactta
    43081 gccatgagat gtaatgtcaa ataagcaatt tcttcttcgg gaaacgatac acctgcgatt
    43141 acctcaatac gttgaacgat ccgctgcgcc acttgatact ctactcgctt agaaacttct
    43201 tgttcgatgc ctaattcctt gatttcaaac ccttctcgga gccgtttgat ccccaaagcc
    43261 aagtgtaaag tcagattctg tatgataaaa tccgaaactt ttaacttagc ttctcttgtt
    43321 tcatcgagaa tgataatgac tagctcttca aaactgatgt ctgggaaaaa acggctttgc
    43381 cctaaatatt ctttcaatga atttccataa tggtttccaa aaaaagtatc catgataaaa
    43441 tgccgtcgat ttctttcttc cccttcgatc catgtccctt gaccatgctt agaaacgatt
    43501 tttagtgaat atggaatcaa tcgctccttg atttcttgca tgtcctttgt caaactggaa
    43561 cgactgatat aaagctcttc tgccaaatca tccataaaaa gaatggcatc ttccaaaagc
    43621 aattggttca atatatactt ttgtcgatct tcggactcat aaaatccttg cggttctcta
    43681 gcatatccag gtactatttc ttgccgactc aaaaacaagt caaatgtcag tctttgtaaa
    43741 atatgcagtt ggtagccata acctggttta gcaatgatct tacctccgtt tttttctact
    43801 aactctttga tccgctgcaa gtaattacgg attgtccgat cagataataa caattcagaa
    43861 gctaaacgtt gactggtgac atactccttc ttgtgatcaa tcaaatatct caacagcaat
    43921 ttttccttct gtttcattat catccactcc tattccatca tatgttctct tcgatcacct
    43981 ctgctaactt ctggatgcct gtagggattg ggacatacgc ttggaaaggg atactaacaa
    44041 caggctttcc aacagattct cctaattttt tgaatttatc caaaaacatc gtcgtttgcg
    44101 ggctgatcag aaataaatca aaatcactat tcttaatcat attatcgcct tctgtagctg
    44161 aaactgcatc tacgagtacc tcttctcttt tttgcttaaa gaaatccgtt gtctttgagg
    44221 ccatcatcga ggatgacatt cctgccgcac aaataatcaa tgcttttttc ataaatcaat
    44281 cactcctttt atttttataa accaagcata tcttgtaagc gttttctatt caaaatatat
    44341 atttccgtag cattgcggaa aaaatgtgta tcatatcttg aataataatt tctggcaccg
    44401 tgatatacta gtgatagaat tttttcagga ggggatttag tgagtatcac agtgaatatt
    44461 ttgtatacaa gtacaaatgg acaagccaga aaatttgcag aagaaatggt cgaaagaaac
    44521 attgtcaaag cgattcgtgc agaagaagga aacgagaaat atgactatta ctttccacta
    44581 gaagatccag aatctctttt actgatcgac cgttggaaag atgaagaagc aatcgaaaag
    44641 catcacaaat ccgaaatgat ggctcaaata aacgaactcc gtaaaaagta taaattaaaa
    44701 atgaaagtcg aacgtttcac tgatttaccg taaaaagata cacatggaat gatagcaatt
    44761 tatcatccat gtgtatcttc actttttcct tattttttat tcgatctcat ctaatgattc
    44821 aaaccgtgtt tttctcttat ttttcggata agtatttcag tcaccccaat atacatgatt
    44881 cctaaacata ccccaaggat attctcaaaa atgcggatcg ttactgctcc tggaaccccg
    44941 aataaactgg cagtgattgc caaagcacca aaagaattaa aaactgtttt atatctatag
    45001 gtagaacaaa cacctaaagc tagaccacct aatataccga cccagttaaa aggaataaat
    45061 tggctgatac caataaataa aacacatcca atcaatgagc caatgattcg gtctactgct
    45121 cgttctttga aaccaatcga catcaatcca tagctagata caatagaaga aaatgcaaac
    45181 cctgcccaca tcaatcgctg aaaagggaca tactctcctg caacgatcaa aagactaatg
    45241 ccaaatgcat agatgagcat ccaaattttt tcctgcgaat acatgccttt caaaaaaatc
    45301 tctttaaaca agcttttgtc tttgttcttt tctcgatgtt ttctgtaaag gagaactgaa
    45361 aaccaacaaa aaaacaaaaa taataagcta cttgtttgtg taaagaaatt cttgttcaac
    45421 gaatctttag gtaacgaata aacaataaag agatatgaaa agccgtacaa agaagcattc
    45481 cccattttgg gatcactagc ggttgccatt aatatgctgc ttagtaaaat aaaatgaatc
    45541 aacatgccca taactgacca tttgatcagc tggacatacg gtgctactaa tagtgagaac
    45601 atgacgattg ctaaacctat gatcgaatgg gaaatcttat aaccaaagtc aacaaaacga
    45661 atactcatca acatacagaa taaaaccaca gcgtatggtt tatgcttcga ttcaaataat
    45721 gttgtaatta taatgataaa acaaatggca aacaatacga tgaataatga tcgtaaaaac
    45781 aatgaacaaa tatacatgca cttttctttt ttagaagctg cttctcgtat tttttgtttt
    45841 aaaataaacg gatctagttg tagagactga taaaaatcca cctgttgtcg tcctttcttt
    45901 ttacttttat tttttacagt catctatgac aggttgtaaa tactatggaa catcatatgg
    45961 cgttgtcatt ttcttgtcaa ctatttttcc tattcatgtt tttataaaaa aacatatcgt
    46021 tttcttgtat gattaaatca ccacaaaata aacaactgag agaacgatat gctttatgaa
    46081 tggttctatc aaaaaaattc tgagatcaat agacaaaaat ttgatgatta cagaggtctc
    46141 ttacgaaacc ttacagaaga aaaaaacatt gatcgttgct gcagttctct tccctgctcc
    46201 atgtgtctgt agaaattgtg gttctgctgt ggtagatgga aataggaaat taatggtggt
    46261 gaaaaacaag aagaaggaaa ccattgtcca ctttgaataa tataatcata tgccaatggt
    46321 tatacgccta aaaaagcagc gctatacctg taaaaactgt cgaagccatt ggaacgctca
    46381 aagttatttt atccgtccaa ggcactcgat ttcaaatcat gtcagacata aaatcacttc
    46441 tttactgact gaaaaagtgt cgttattttt tatttcgaaa agttgttagg tatctttgac
    46501 aaccgttatt cgcacattga aagagttgag aagctattta ccaaagtctt ccaaaagaat
    46561 tctgtcttga gtgttgaggg ttgtgaattt cgttctcacg tttccgttga ggataaactg
    46621 agttttattt ccgcggatgg cgagacagaa aaattaatcg agaaaattta cctacacaaa
    46681 aaattcctcg attaacaaac tatttcctta gctgcactaa tcctaaagaa atagaattct
    46741 tagttacaga catgaacgcc acctactttc agctcaccaa acatgttcta ccaaatgcga
    46801 aattggtgat tgatcgatta cttggttttt ccacgccttt caaggaggcg taacctttat
    46861 tcacgagttg gttgaagctt tccgagacaa aagacccgac ttgtttttct ccttattgac
    46921 aaagagccat taaaactaga gaaaaaatta caactgatcg ctgattattt gtagaaagaa
    46981 aatcaaagta ttacccaata aacttgagtc attaggcaaa aaacactgcg aaactcaaga
    47041 atgattttcg cagtgttttt gtatattttt tattaactag tattactaca aaggtttatt
    47101 tttcacttct caacgcagca caaaattgtc tttacttatt tactggttgg gttcaacctt
    47161 ctctactccg atactaatat ttgtttgtat accccttcta aaatcggtag tttccattca
    47221 attgatgggt actagttgat tttaaaaact atgcttattc aattttttat tttagctcat
    47281 atatttcgtt tctttctaaa ttttatactg atgaaaacaa gtagcacacc caatattgac
    47341 aatataaact ggctaatgat ggtttctcca gtttttggaa gatgcttatt tccactgctt
    47401 gttggattgt ttcctcttat atctgagcta tcagcaactt ttctattctc ttttgttaaa
    47461 aagatcgcca cttcttgttc tttttcgatc ttgaaagtta ctggtgtcgc atccaattca
    47521 taccccgttg gcgcttgagt ttctaccagc tgatatgctc ctggtgctaa gccatctatc
    47581 gccagttttc catctgctcc tgtgg
//
LOCUS       DACCKU010000011        46372 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.011, whole genome shotgun sequence.
ACCESSION   DACCKU010000011 DACCKU010000000
VERSION     DACCKU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 46372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.011"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            53..1012
                     /locus_tag="IVN13_000564"
     CDS             53..1012
                     /locus_tag="IVN13_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7011081.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            1182..1862
                     /locus_tag="IVN13_000565"
     CDS             1182..1862
                     /locus_tag="IVN13_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7011082.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            1952..2986
                     /locus_tag="IVN13_000566"
     CDS             1952..2986
                     /locus_tag="IVN13_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7011083.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            3000..3335
                     /locus_tag="IVN13_000567"
     CDS             3000..3335
                     /locus_tag="IVN13_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011084.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            3319..5799
                     /locus_tag="IVN13_000568"
     CDS             3319..5799
                     /locus_tag="IVN13_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011085.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            5855..7414
                     /locus_tag="IVN13_000569"
     CDS             5855..7414
                     /locus_tag="IVN13_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011086.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            7411..8775
                     /locus_tag="IVN13_000570"
     CDS             7411..8775
                     /locus_tag="IVN13_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7011087.1"
                     /translation="MKIVIIGASHEGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            8946..10016
                     /locus_tag="IVN13_000571"
     CDS             8946..10016
                     /locus_tag="IVN13_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011088.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            10031..10699
                     /locus_tag="IVN13_000572"
     CDS             10031..10699
                     /locus_tag="IVN13_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011089.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            10719..11297
                     /locus_tag="IVN13_000573"
     CDS             10719..11297
                     /locus_tag="IVN13_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7011090.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            11698..12609
                     /locus_tag="IVN13_000574"
     CDS             11698..12609
                     /locus_tag="IVN13_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7011091.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(12987..13904)
                     /locus_tag="IVN13_000575"
     CDS             complement(12987..13904)
                     /locus_tag="IVN13_000575"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP7011092.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELDKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            14106..14543
                     /gene="zapA"
                     /locus_tag="IVN13_000576"
     CDS             14106..14543
                     /gene="zapA"
                     /locus_tag="IVN13_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP7011093.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            14601..15149
                     /locus_tag="IVN13_000577"
     CDS             14601..15149
                     /locus_tag="IVN13_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP7011094.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            15264..17624
                     /locus_tag="IVN13_000578"
     CDS             15264..17624
                     /locus_tag="IVN13_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP7011095.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKIENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNTYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            17711..18025
                     /gene="trxA"
                     /locus_tag="IVN13_000579"
     CDS             17711..18025
                     /gene="trxA"
                     /locus_tag="IVN13_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP7011096.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            18207..19985
                     /gene="uvrC"
                     /locus_tag="IVN13_000580"
     CDS             18207..19985
                     /gene="uvrC"
                     /locus_tag="IVN13_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP7011097.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVICEGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMVKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            20130..21029
                     /locus_tag="IVN13_000581"
     CDS             20130..21029
                     /locus_tag="IVN13_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011098.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            21022..22284
                     /locus_tag="IVN13_000582"
     CDS             21022..22284
                     /locus_tag="IVN13_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011099.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPIRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            22445..24343
                     /locus_tag="IVN13_000583"
     CDS             22445..24343
                     /locus_tag="IVN13_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP7011100.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(24673..25392)
                     /locus_tag="IVN13_000584"
     CDS             complement(24673..25392)
                     /locus_tag="IVN13_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP7011101.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLVEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            25729..26061
                     /locus_tag="IVN13_000585"
     CDS             25729..26061
                     /locus_tag="IVN13_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011102.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            26048..28051
                     /locus_tag="IVN13_000586"
     CDS             26048..28051
                     /locus_tag="IVN13_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP7011103.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            28070..28540
                     /locus_tag="IVN13_000587"
     CDS             28070..28540
                     /locus_tag="IVN13_000587"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP7011104.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            28561..29145
                     /locus_tag="IVN13_000588"
     CDS             28561..29145
                     /locus_tag="IVN13_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP7011105.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            29160..30146
                     /locus_tag="IVN13_000589"
     CDS             29160..30146
                     /locus_tag="IVN13_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP7011106.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            30136..30447
                     /locus_tag="IVN13_000590"
     CDS             30136..30447
                     /locus_tag="IVN13_000590"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP7011107.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            30469..32250
                     /locus_tag="IVN13_000591"
     CDS             30469..32250
                     /locus_tag="IVN13_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP7011108.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            32243..33619
                     /locus_tag="IVN13_000592"
     CDS             32243..33619
                     /locus_tag="IVN13_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP7011109.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            33626..34261
                     /locus_tag="IVN13_000593"
     CDS             33626..34261
                     /locus_tag="IVN13_000593"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP7011110.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            34291..35646
                     /locus_tag="IVN13_000594"
     CDS             34291..35646
                     /locus_tag="IVN13_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP7011111.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(35838..37232)
                     /locus_tag="IVN13_000595"
     CDS             complement(35838..37232)
                     /locus_tag="IVN13_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7011112.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            37324..38295
                     /locus_tag="IVN13_000596"
     CDS             37324..38295
                     /locus_tag="IVN13_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7011113.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            38292..39074
                     /locus_tag="IVN13_000597"
     CDS             38292..39074
                     /locus_tag="IVN13_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP7011114.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(39243..39614)
                     /locus_tag="IVN13_000598"
     CDS             complement(39243..39614)
                     /locus_tag="IVN13_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7011115.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            39748..40191
                     /locus_tag="IVN13_000599"
     CDS             39748..40191
                     /locus_tag="IVN13_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7011116.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(40234..41043)
                     /gene="yidA"
                     /locus_tag="IVN13_000600"
     CDS             complement(40234..41043)
                     /gene="yidA"
                     /locus_tag="IVN13_000600"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP7011117.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(41062..42429)
                     /locus_tag="IVN13_000601"
     CDS             complement(41062..42429)
                     /locus_tag="IVN13_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7011118.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(42413..43243)
                     /locus_tag="IVN13_000602"
     CDS             complement(42413..43243)
                     /locus_tag="IVN13_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP7011119.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLEDAGYAPVIRNSGGLGIVSDEGILNISLIFPKSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            43406..43843
                     /locus_tag="IVN13_000603"
     CDS             43406..43843
                     /locus_tag="IVN13_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP7011120.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            43919..44539
                     /locus_tag="IVN13_000604"
     CDS             43919..44539
                     /locus_tag="IVN13_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP7011121.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            44728..45234
                     /locus_tag="IVN13_000605"
     CDS             44728..45234
                     /locus_tag="IVN13_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP7011122.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            45251..46150
                     /locus_tag="IVN13_000606"
     CDS             45251..46150
                     /locus_tag="IVN13_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP7011123.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            46237..>46372
                     /locus_tag="IVN13_000607"
     CDS             46237..>46372
                     /locus_tag="IVN13_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7011124.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     F"
BASE COUNT        15300 a         7967 c         9780 g        13325 t
ORIGIN      
        1 tgtgctacac tcaaaatagt tccgacgaaa gaattataag gagtgagtac aaatgaccta
       61 taaccatctt acaccaactg aactcgtaat gatagaagct tatttcaatc aatcacaacc
      121 tgtttcaaaa gtagccaatc tgttacaacg ttctcgacaa actatttata aggtctatcg
      181 ttttcttaaa tctggtggaa gcgcaatgag ctactataaa caatataaga aaaacaaacg
      241 taactgcgga agacatccga ttgttttacc tgatgagcaa caggaataca ttcaaaaaaa
      301 ggttgttcaa gggtgggctc ctgatgtcct tattggtcga gcagagtttc ctattgcttg
      361 ttcggtacgc acactgtatc gaatgtttaa agataaaacc tttgacactc acgatttacc
      421 aatgaaggga aaaagaaaac caaacggtca taaagaaaaa agaggaaaac aagggtttcg
      481 ccggtcaatc cgagacagaa agacagacta tacccagttt gatcatgaat ttggtcacct
      541 ggaaggtgac actatcgtgg gtggaaaaca taaaagcgcg gtcatcacat tagttgaacg
      601 gctatctaaa gtcatcatta ccttgaaacc cgaaggcaga caggctaaag acattgaaaa
      661 taggctgaat caatggtttc gttgtgtccc aaaaaactta tttaaatcca tcacatttga
      721 ttgtggaaaa gaattctcaa attggaaaaa gattagtaat accaacgata tttcaattta
      781 ttttgcggat ccaggaacac cttctcaacg aggattgaat gaacattcaa atggcttatt
      841 acgtaaagat ggtttgtata aagctatgga ttttaatggt gtatctgaaa cctttatcca
      901 atcggtggca tcgaaacgaa atcatattcc tagaaaatca ttacactatc ggacaccaat
      961 ggaagtattt ttgagttacg tcacaaaaga tgatttgtct aacttaattt gacaaacgaa
     1021 acttaataaa aaaggaagaa tattcatgta aacgatttta ttgttttttt ctcttttata
     1081 caaaaagggt cataatttca agtaagcgga tgaattcatg aggatttttt gcatactgtc
     1141 tctactatac ttaggtcact aagaaaaagg agtgatttat tatgaaaaag aacatgattg
     1201 ggacattatt actttcaaca cttcttctag gcggcttggc aacaccagct tttgctgagg
     1261 gtcaagcgac ttcaaaagga gatattacct ttactgaacc aacaaatacc gtggagcctc
     1321 ttaaccctac tgatccttca aaaccagtag agccagcaga tccggaaaat ccagcaactg
     1381 gccagacagg tcctttaaca ctggatgtag ttcccgaatt gccatttggt acacacgaga
     1441 ttgaaagcgg aacaaaaacg tatcaagtag acgcttcaaa aaacgataca ccttatctcc
     1501 aagtatcaga tcgtcgaggt gtaggtgcag atggccaagc acaagggtgg aacgtgacag
     1561 tttcggtttc agattttgta aacggttcac aagttttgca aggcgctgaa ttagattttg
     1621 gaacatccac tgttaaaagt acgtcagata acgaatccac tggaccaact agtcaaactg
     1681 taactgggtt gagtaaagca agtgcagcga caccaatatt tacagcagca aaagatcaag
     1741 gtctgggaac atggctttct gtttatgatc cagcaaatat cacactgaaa gtgccgaaag
     1801 cagcagctgg aacattcacg gctgatttga cttggaacct cgtggctggc ccagtagcat
     1861 agagtatgaa ataaattcaa caaactgcgg tgaaaatact ataggtgaag gattttttac
     1921 cgcagttgtt gttgaaagga tgtggtggac aatgaatcaa agtaaacgac tgtttgtttc
     1981 gtttttttct attttaagtc tatttttcat ttttccgtca atcagccaag caaaggattc
     2041 agcaggagac tttggaatca aaccggtatt tccagagaac caaatagata aagcaatcgg
     2101 ttattttgat ttattagtgg caccggaaca aaatcagaca ctagaagtaa ttatcagcaa
     2161 ttcttcagat gaagaacgaa catttgaggt ctctgtcaat ccggcggtaa cgagtgatgg
     2221 tggaacgatc gattatagtc aaaaaaaccc aacgttagat gagacattgc cgtttgatgt
     2281 ccgtgacgtt ttgctcattg cgaaaaagga aatcaatgta tcagcacatg cagaaacaac
     2341 tgtacctata gaagtgaaaa tcccagcaaa aagctttaag ggacgtgtat tagcgggtat
     2401 tcatgtttca cctaaagaag aagctgaaac agaaaatgct acagaaggag cacaaatcaa
     2461 aaatcgtatt gcttataatc tcgcagtcgt tttacaagaa agtcaagaga cgatagaacc
     2521 ggatctgaaa cttttatcag gagatttgga cgaggtcaac gcaaagccaa ctgttcagct
     2581 tcgtttccaa aatcctcagc ctaggattat cagcaatttg atatttacga gcaagatctt
     2641 ttatgaaaat cagctttata ttgaaaatac gtctaatgca tttttagttg caccaaattc
     2701 gaattttcac ttgaatcttg atttagctgg tgataaagca aaagccggcg attaccgcgc
     2761 agaaatcata gcaaaatcag gcgacagtaa tgaatggcga tttacacaaa actttacgat
     2821 caaaaaagaa aaagcacaaa aagtaaatga aaattccgtt tttgcagtac aagaacaatc
     2881 tttcccatgg ctttatgcag gattggttgc tgcagttcta gtgttatggt tgattttgct
     2941 gtttttgtgg gtccacaaga aaaagaaaaa aggagaaaca acatgatgag gtgatttcgt
     3001 tgaaaacatc gatcttattg ggagggatta ttgcaagttt gctgttcttc agctggcaag
     3061 agccagtcca agcagatacc aaaagttcag gtacgattac attttatgat ctaagtccaa
     3121 ttccgcatcc aagtggttca gtcaatggcg catcaaaaca gcagacacag ctaccgattt
     3181 ataaagaatc taatcaacat ggcgtattcc cggagacgaa tgaacaaaag atccgttttc
     3241 tttatttgat cattggtata gaattatggc ttcttgcgtg gttgttgtta tggagaagaa
     3301 aggaaaagga ggatgaacat gttgctacaa aataaatttt cattttttcg ctccttcttt
     3361 tttacttcgc tttttgtttt aagtatattt atcttttcac cttcggcaga agctgttgga
     3421 gcaacgcctc ctgccatttc ttatgatcca tacacgtatg aaaatatcgt acctccaaat
     3481 ggtggacgta gtgcagtcaa tggcgggaag cttgtccaac taactggggc taaccaattg
     3541 cctaaattga ctgctactta tttgaacgat ggttcaaatg atccttggaa aggtggcacg
     3601 gtccgttttc attgggaaag ccatgtgaaa ctaacaaatg tacttgattt ggtgaatatc
     3661 actgcacaag gatttgcatt tgtggcgatc atcaatttgc cggaacatgt atcaagcagt
     3721 gatgtattaa aagcaattga ctggaacaac gcctacttgt atattgccgg tgcccccatc
     3781 aaggttacta gcggaagttt attatcagca ggaagccata ccttgcgttt tgggatgggg
     3841 tcgtgggata caaatactct attgacagat attattgcta ttattacgcg aggcgaattc
     3901 aacgtggacg atatcccgct tcaatttgat gttacagcta atgtcgcgaa gatgacagaa
     3961 aacggagata aatatgatac gagtccaaac aaaattttga ccaagggtaa attacaacct
     4021 tctatgtcta aaaaggccgt tttttctgtt gatttttatg attcgaataa tattatccaa
     4081 gatcgtatgt tcaattcgat tttagctcca gctgcaggta gattttatcc taaattaact
     4141 ggcggaaact tgaatccagg agcatatgca ttttctgcaa gtgcagagat aaatacgtgg
     4201 aatagttaca tctcaccaag tgattcaaca ggaaaatata atacatttgc tgataccaca
     4261 gaatacgtgg atggaaataa ccagaacctt ccaggctcta ccaatagtcg gacattgacg
     4321 atggggctga ctgaaggacg ttttgatcag ttgccattta gtcgctttga tcgcgtagtc
     4381 aactatttta caggaaaaaa cgttacacaa ggagcaatac tgactcatac tccagcaagc
     4441 acaagtggat tgaatcagac agccaatatc gtgtacagtg ggaaagatgc gcaaggcgga
     4501 ttgctttctc ctgttgcttt aaaggtattg gagcagtaca agctagacgg aacggtttta
     4561 ccaacaaact taacagccga tagtcagtgg ggaaagctcc tttcttctgc atcgtaccaa
     4621 gtcacggcca attggtcagc aactgaacta aaaaagggtt cgatccatta caggctttat
     4681 tcgaaaggaa cccaaaaatt agcgacagga tggacagatc aacttttcca aacgatccaa
     4741 tcaaataacg ggggtgtaaa tacagctact tctacactac ctgcattggc ttcagggcaa
     4801 tattattttg attatcgctt ggtggacgat gtacttacta acatgtatcc gtcttttgcg
     4861 tacaaatggc agtctgaaca agctggaatc accactcttc ctcaaataac agtggctaat
     4921 tttccatctg tgagtgcaga tagtcagcta aaaaacctta gcagacaacc tgagacagga
     4981 gatcccctgt ctgctctatc aggagatacg attcaagaaa atgtgacatt cacgttaaca
     5041 aaatctgggg atgcgataac cgacaaaaaa atcacgatct cacttcctga aaatactact
     5101 tatatcaatg gttccttaaa attaaatggt acaactatcg cagataccgg cattcaacaa
     5161 ggagtttctg tcccagcaga tctattgagc aaaataggag ataccattca tcttacttac
     5221 aactatcaat taaatactgt agatacttct ttacaatccg tcagcatact gacaaaggca
     5281 gctgtacttt ccagcaatat tacattagca gatggtgcta agcttcctaa cccagttgtc
     5341 caaacatctg ctaaaacaat tcttgtcccc aaacaagaat taacattagt taatgtacca
     5401 gatgatttta catttggtaa cgatctcccc aaaccgttga agacaagtta ttatgaatca
     5461 aaaggcgatt tttcttttga tgtgcgggat actcggcttc caagcacgtc tccttggcaa
     5521 ctgacaggta cattgacttc tttatttaag aatgatcaag gtcaagaatt aagcggaaca
     5581 aaattatact tcaatcattc tggatcaaag caattgattc agcaaggtca aaacacgttg
     5641 atttatgaga gtgatggaac agctaaagga gaggttctcg ttgactttcc cgataccgat
     5701 ggtttactct tagaggtaaa tagcagtacg aatgctcaac ctggcgcaac gtatcaaggc
     5761 atggtcacat gggaactgac agctgggcca accagctaag tgattagaat ttgtatggat
     5821 ctgtgataac tgtcacagat cctctttttc aaggatgaga aggaaacttc tgctgttaaa
     5881 atcagaaatg cttgaaggag tggacttgga acttttcagt ttgcttgaaa aaaatgaaca
     5941 acggcaaatc aaacttttcc aacttttatt agaggaaaaa ggagaggttt ccgttaataa
     6001 cgtacttacc cgcttatcag ttgatcgggc taccttgaaa gaagatctgt tttatttaca
     6061 agctaacttg gaaaatatca aacaattgga aattgttatc caagaaggga atattttatt
     6121 aatcagacat ggaaatatag cagcagtcga tgtttattat cattgttact tgcgctattc
     6181 tacaaaatat cagatacttc tttatctgtt ggaaaatggc tcatttgatc ggcagaaact
     6241 tgtcatggaa cttaatatca gttcggctac tttgtctcgc agagtgaagg aattaaatga
     6301 ttctttaaat gagtttcatc tgaaaataaa aagcgggcgg ctgaccggtc cagaaagtca
     6361 aattcgttat ttttatttcc aattattttg gtttggacgt ccctatcttg aaaacaaaaa
     6421 agaatacagt gatttatcag gaaacacttt tttagatctg ctgcaaaaag agtttagttt
     6481 tgctattaca gaagaaggac gattgaaatt tgaaatatgg ctttccatta tgaagaagcg
     6541 aatgcgctgt acgaaaaaat ccaaaccaga ttatagcaat cttcctgttg gattgtttca
     6601 aacgacacaa tttttattgg acctccatac atttttgacc cgttacttct ttcaatatgc
     6661 ctttccttgg aatcaaaatg aaacgttggt tttttatctt ttcatgatca gtaactttac
     6721 attagataca cataactcgc atgtccagga gtttttgata agcgaaaaat acaaagacga
     6781 cgtagtagct caattgaatc gaacgtttaa acaatcgata gaagaatatt tctcttttta
     6841 tgtttttcca gaagagattt cgcaaaagat tgcgttgaca gtgatgcaaa atcacgctca
     6901 ttttgcctat tttagaggat ggatgaatat ttttggccaa gaaggattaa aacagcggtt
     6961 aactgaagta gaagataaac aattcctggt tttatgtgaa agattggtca gacaaacatt
     7021 tgctattgtt gattttactg aagtagacga ggaaaatgcc cgctttgaat tatttgctcg
     7081 ctatgcatct attttacgga cgctgatcaa gcagttagaa ttacgtctgc aaattgctta
     7141 tgatcttcct tatgaaaggc taatggcgga tatcgtgaaa gaaatcatcc aggaacaatt
     7201 gctgaatacg atcaaatatg atttagtcga atacgagaaa gaaaagcagt atgatatcat
     7261 tttgactagc caattgaaag agtacccgtc acagaagtcc gctaaagttt ttgtattcac
     7321 tagcaacgaa tataattatg atttccccta tttgaaccaa tttttaaaag aatgttattt
     7381 agaaaaattg aatttaagga tgaataaaac atgaaaatcg tgatcattgg tgcttcccat
     7441 gaaggcatca cagctgccct aaatctaaga aaacttcaac cgaaagcaga agttttactg
     7501 atcgatgaag atcataaaga tggactaggc tatgtttcaa atggaatcaa cctttattta
     7561 aaaggaaaga tacgtagttt agctgaagtt gcccataaca tgcggactct tcaagaaagt
     7621 ggggcaaaac tgatcaccga atggagagta actgaacttg atccagacaa acatcagctc
     7681 atcctcgcat ccaaagaagg aaaaaacgag accataacct atgataaatt aatcgtagcg
     7741 acaggttcct ctcctgtcac cttgtataaa caaattgaag ctgaaaatgt ctacacctat
     7801 aaaaatctag tgcaatctaa gcaagtactt gccgctttaa aagaagcaaa agaagtagtg
     7861 atcttcggtg ctggttacat tggattggaa ttagcagatg ctttgagaaa taaaggatac
     7921 attattcatc tagttgatta tatgccgaat gttttatcaa gatatttcga taaggatatg
     7981 atcaacagtt ttcaaaatca gctgcaaact aagcaaataa atttttatcc aaatgaattc
     8041 ttgatcgatt ggaaaaaatc agaagagaaa gtagtatccg tacaacttct gtctcaaaca
     8101 ataaaggcag atatggtcat tttttctgcc caaactcgtc ctaatacgac attgttaaaa
     8161 gaaaaagtgg ctttgtatga ggatgagact gtgatagtca atgaatatct gcagacaagt
     8221 gatccggaca tctatgcgat tggagatatc gtcccagtca gttttgacaa aaataaacgg
     8281 catctctttt tacctttagt tactcgagct gtacatatgg caagagcagt agcgctaact
     8341 ttgagtggtc agccaaccgc ttacgacctc agacaaaaga taacagcagc agtcattata
     8401 gactactttt taggaactgt agggctgaca gaagatgaag cgccattctt ggaacaaaat
     8461 accggttcgt gttcaggaga atttgatcta tttccacaat acgacgaaga aaacaaaaca
     8521 gtaaatgcaa agctgatcta ccatccggat acattagaaa taattggcgg acagctgata
     8581 agccaagaat tcttattatc agatttgaac ctgcttgcgg atatcgtgaa acacaaaact
     8641 acgattccac agctagcagt tgaggatttt ggatttctgt ctgaatacac ctctcgtttt
     8701 cactacctca atgagttagc ttttaaagta ctagctaaga gagcaaatag caatagagta
     8761 gataaaagac tgtgagaaat atcacggtct tttattgtgt ggggatataa taaatattaa
     8821 tcaatcgaga ataaaaaatt catataatga aatagcggaa gaattttttg agcataatat
     8881 tcgccaaaaa tttatcaagt gataaaattg ttgtataaaa tgattatttc aggaggagga
     8941 gttaaatgtt tttagcatta aatgaaatta tgcattcaaa gttacgttat gcgttagttg
     9001 caggggtgat gtttttgatc gcctacctgg tatttttttt gactgggtta gcttatggct
     9061 tagctcaaga taatcgaaca gcagtcgaca agtgggaggc agattcgatt gtgctttcga
     9121 aagatgcaaa ttcaaatcta gggatgtcca tgattactaa gaagatagca gaagaagtcg
     9181 aaggtgggaa agtggcgtat cttgcccaaa cacctggagt cgtgactagt aaagacagta
     9241 cagaagaagg aaaaatcaat gttagcttct tcggtatcga taaaaatcaa ttcatcatgc
     9301 ccaatcttgt tgaaggaaaa gcttttgata acgatgatga ggcagtaggc gatattagtt
     9361 tgaaagaaga atatggtcta gccataggag atacagttaa gttatctggt agtgacaaga
     9421 cgtttaaact aactggcttt acggatcatg ccaaattcaa tgtctcacca gttctctata
     9481 caaccatcaa tgcttaccaa gaaatccgct tcgaaaaaga agatacaagt gaaaatgcac
     9541 ggattaacgc aatagttgtc agaggtaaaa tcagtaattt acctgaagat ttagaacaga
     9601 tcaaaatttc taaattcatc aatgaattac ctggatataa tgcgcaagta ttgacttttg
     9661 gatttatgat cggatttttg atcgttattg cggctattgt gatcgggatt ttcatttatg
     9721 tactgacgat gcaaaagatt aatatctttg gtgtaatgaa agcacaaggg atcacaggag
     9781 gatttattgc gcgttctgta gtggcacaaa cattcatttt atcatttgtt ggtattcttc
     9841 ttggtctatt aggaacagtg ggaacatccc ttgttctacc tgatgcggtt ccattccaaa
     9901 gcaattggct gttttttggc gtcattagct tgttgatgtt agtagtagct gtgttaggcg
     9961 cactattttc tgttcggaca atcgtaaaaa tcgatccgtt gaaggcaatt ggataagaag
    10021 gaggaagaac atgaaagcaa tagaatttaa agaagtagaa aaaagtttcc aagatggcga
    10081 ccaagtagtt aaagcattga aagaaacaac catgagtatc gataaagggg aatttgttgc
    10141 aatcatcggt ccttctggtt caggtaaaag tacttttttg actttggcag gcggattgca
    10201 gacgcctagc agtggctctg tatttatcaa tgatcaagaa ttcagtaatc aaaaagaaag
    10261 cgaacgcgtt aaactacgtt ttaaagaaat tggctttatt ttgcaagctt ccaatctggt
    10321 tccttttctg acagtaaaaa agcagttatt attagtagat aagattaaac atgaacatcg
    10381 tcaaaaagaa gcaaacgaat tatttgagca gcttggcgtg gataagttga tcaacaagta
    10441 tccagaagaa ctatctggtg gagaacggca aagggtcgca atcgctcgtg cattgtacaa
    10501 tgatccgtcc attattctag cagatgaacc aactgctagt ttggattcag aaaaggcacg
    10561 agaagtagtc gatatcttgg caaaagaatc aaaagacaaa aataaagcaa cgatcatggt
    10621 tactcacgat acacggctga ttgacaaatg cgatagaata tttatgatgg aagatggtgt
    10681 attgaaagaa aaagaataac caaaaagcag gtggaaaaat ggtccatcga tacaacaaag
    10741 atacacagca aaaaattttg aaagaagcga ctgacttgtt tatgtccaag ggatatctcg
    10801 gcacttcaac aagagaaatc gcacaaaaag ttgggatcac ccagcccaat ctctatcatt
    10861 atttcggtga taaagaaaaa ctatatacgg cagttttaga aaaccactta aaagatgttg
    10921 gaaaagcttt gagagaaatc gttcaaacaa gcgaaacggg atttcaagta accttgacaa
    10981 aaatggcgca gtttttgatt gagacacatc tggtcgattt gtttctgatg cttcacgatc
    11041 ttgaaagcaa tctttcaaag gaaacaagag atcatctttt ctttctatgg aagaaaaatt
    11101 atcgtgaacc ttttgaagaa atcttttcag caaaccaatc tgtgctaaga aatgggattt
    11161 caaaagaaat cgcagcaagg cattttttct tagtgttagc accttacatc actcggtcga
    11221 aaagacctgt ggaagaaccg ctaaatgtgg cgcaattgat cgatttatat ttgaatggtg
    11281 tattaggaga agcgtaaagc tgttatatag ggagaaaata gttaggtgaa gaagcaagat
    11341 atgacgaaga tcgttatatc ttgcttcttt atataggtag tttttcataa atacaaattt
    11401 gttattctct catgattatc agggtatatt tttatttatt atgtcggtgt tccaggaagt
    11461 gttttaattt aataacgaag aaattaattg tttgtagaat gaaggaaact atttattggg
    11521 tagatgaatc accacgaata gtcaaaagga gatttcagga tatgccaaaa atgaaaaaaa
    11581 tcattttagc agtaatgtta ttttctacgg ttatatcttt tacagcccct tttttgctta
    11641 cttaagctga ttttaagttt tggcccttag tctggatcta cgaggtgaga gtcaagaatg
    11701 aaaaaaataa ttggagcttt tgtatttgca atcattatca ttattatctg ttttcaaata
    11761 gatgtttttg gtcaaaaaaa gatagttgga caaacggtca gcagtacgaa cagtacggtt
    11821 acaaagacaa aggaatcatt tgatgataca gaacaagaga cattaatcaa aatcggaaaa
    11881 aatgaccgta cactatatac agatgactcg ctaactaccc cacttgcaac tataaacggc
    11941 ggagagctgg cggagtttct ttctgaaaca aaaaatgctt atcagatcaa gacaaatgat
    12001 ggctatactg gatatttagc cctcgaagat ggaaccaaag tcacaaagaa tatacaaaca
    12061 aaaccggaag agctatctga tgcagtgatt gtattagatc ctgggcatgg cggaaatgat
    12121 actggggcat taagcaatga tgaacaaaca gaagaaaaag atataacatt aagtacagcc
    12181 ataaaagtaa aaaaggcgct agaggatgct ggagcaacgg tttatttgac ccatactaca
    12241 gatgaattag tacagcttgg agatatatgc gactacagtg aagaaaaaaa ggcagatgtc
    12301 tttatcagtt tacacgcaga ctctacggaa tatgcgaatg aagcaactgg aatcacgaca
    12361 tattattact atggacaaga ggaaacatta gcacagacaa ttgctgactc cttcactgat
    12421 ctgcctctgg attcaagagg aatctccact gggaactatc aagttcttcg agaaaatctt
    12481 caaccttcta ttttaatcga gatgggttat atgaataatg atagtgattt aaaggaatta
    12541 gtaacagaca gttatcagca gcagattgct gcaagtttga cacaagcatt gaccacttat
    12601 tttcagtaat aaataagccc agccaaacaa aaatagctcc tcatattttt cgtttggccg
    12661 ggcttattat cgcagatgca agcttttaaa caccgtttaa ggaagaggtc gtgagcctgc
    12721 cgttcaactc tgagtcacaa ggagcaattt caaaaaacag cttctcatgt ttgatgacta
    12781 ttgtgacttg tgaccgagga gttggcatga gaacaccgtt taagaaaaag gttgtgaaat
    12841 caacgttttg ttctgagtat tagagaaaca aacggaaaaa tagttcctca tattttgccg
    12901 tttgttgagc taataccgag gatcagatga ttgaacaccg tttattcgga acaagggact
    12961 gtgacatgac ttttgtcaca gttccattat ttttttagta gcttcaaagc tttttctgta
    13021 ttggcaaaat gaagtttggc atatttttca agcataggca tgccgccttt ctccaatagt
    13081 tttgccgcta cttggtcaga tttcgttcca gctcctgacg gaagggtgat tccagcttca
    13141 gctgatgctt tgtctaactc ttccaaaaag ctggcacgac tgatgataga agcagcagca
    13201 acagctaaat gatattgttc tcctttagta acgaagtaca gtttttcttg taattgattt
    13261 ttttctaaac gcgcatattt tttaaaattt gcttccggcg taaactgatc gatcaaaatc
    13321 gcttctggtt tttctggctc gatttttttt agcagcaaat ggatcgcttg attgtgcaaa
    13381 gcgactttca tccgaacggc attgtatttt ggttggatct cattgtattt ttctggagat
    13441 aagacaagca gtttataggg gatggttttt tttaagatgt cagcaagctg gatgatttgg
    13501 ctatctttta actcttttga atctctaaca ccaagtttac gtaattctgc aagctgatca
    13561 cgtttcacat aagcagcaca tactgttagc ggaccgaaat agctgccatt tccgacctca
    13621 tcactaccta aaacggataa ttgactgata ttttttggca aagaagaggc tgtttttgct
    13681 ggcttggctt gtttatcctg tgcctccccc catttatcag cttctattgc tgcattcgta
    13741 ccttgaaaca tcacttttcc ggatgtataa gcggtaatgg tcgtcccgtc tttttttgca
    13801 gcaaagctag tataaggaat ctgtttattt aacaaataag agcgatagta tgctttcatt
    13861 tgactaagtt ggtcgtttgt tgctttgata atgacgttgc tcataaaatt ctcctcgttt
    13921 ttatacataa aactataccg caactcacga cttttcgttt tctaagagat tgcgagacat
    13981 agcatttttt taaagtttat catataatag ttaagtatgt gaaaaatatt tgtggagaac
    14041 ggtgaaagaa ccaacttcat ggtacactaa ataaaacttt catcaaatag gaggaaatga
    14101 ttcgtatggc gcacgaaaaa acacgctaca aagcggtgat tgccaaccaa acatatacta
    14161 ttatcggacg ggaaaccaag catcatatgg atatcgtaac gaagctgatc aatgaacagt
    14221 tagcagaatt gaaacaatta tcaccacaaa tggataatga acaagcagca atcttgatgg
    14281 ctgtcaatgc gctttcggat caattgaaga aacaagaaag aatattggag ctggaagaag
    14341 aaacagcaga attaaagaaa aaaatgatca aatttacaga gcttgaaaat cgtgtgaaac
    14401 gcattgaagc aatcgagaac gaagctcggg aagtattgaa agaaaatggt caagcagatc
    14461 ataccattaa aaatcatgtg gaagcacaac agattttaaa cgagaaacga aaaggacaaa
    14521 ttaaacaaaa atcttctaat taagatcctt ttatcaaaat aagaaaaatt taaaaaagtt
    14581 agaagagaaa ggaacgtagc atgctgagtt tgctgatttt atttttattg ctaattgcgt
    14641 ttttttctgg agcacgtcgc gggtttgctt tgcaaatcat attcgcgatt ggatatgtat
    14701 tgtcatttat tgctgcccag catttttaca aaccattagc aagtcatttg gaattataca
    14761 ttccataccc agctgtcacc ccaacatcaa agttagcatt ttttgatcag gtatttgcct
    14821 ttcacctaga tgaagctttt tatgcaggaa ctgcttttct gattattttg ctaattggct
    14881 ggctgcttac ccgatttgtg ggggtttttg tccatggatt gacgtatgtg ccgatcttga
    14941 gacaggtaga ctggattgca ggaggcatcc tgtcactaat catggcttac gttactatat
    15001 ttttgatttt gcagttactt gcctttgttc cattagatgt ggtccaaaat caattcggcg
    15061 agcatagttt cgctcgtttt atcgttgaga gaacgccatt tcttaccaac aagatgtatg
    15121 agctttgggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    15181 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    15241 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    15301 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    15361 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    15421 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaaattgaa aatatccgtc
    15481 cccatatgaa acggattgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    15541 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    15601 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    15661 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    15721 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    15781 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    15841 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    15901 acgatcaaag tgcttcaggt cagactttat ttattgaacc aaaacaagtg gtggatttga
    15961 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    16021 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatact tatgtgattg
    16081 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    16141 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    16201 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    16261 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    16321 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    16381 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    16441 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    16501 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    16561 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    16621 aagtttatgg atataatcgg gcaaatacga ttaatgccag catggagttt gatgtggata
    16681 cattaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    16741 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    16801 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    16861 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    16921 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    16981 aaaaagcaaa tgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    17041 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    17101 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    17161 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    17221 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    17281 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    17341 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    17401 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    17461 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    17521 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    17581 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    17641 gcagatggtt cgctatccaa gtatagaaat gctatactag tcatagattt taaaaatgga
    17701 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    17761 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    17821 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    17881 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    17941 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    18001 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    18061 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    18121 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    18181 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    18241 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    18301 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggtagc cataatacga
    18361 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    18421 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    18481 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    18541 tggtgattac tcgtaaggta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    18601 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    18661 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgc ctgtgtcctt
    18721 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaactttt
    18781 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    18841 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    18901 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    18961 cgattgataa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    19021 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    19081 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    19141 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    19201 gagaaaagaa aaaattagtt gaattggcga gtaagaatgc ctcagttgct ttgaatgaac
    19261 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    19321 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    19381 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    19441 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    19501 tcatctatcg cagatattcg cgagtgattt gcgaagggtt gcctttacct gatttgatct
    19561 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    19621 gaatcgatat tcctattgct ggcatggtaa aaaacgataa acataaaaca aatgagttgc
    19681 tattcggtac agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    19741 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    19801 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    19861 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    19921 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    19981 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    20041 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    20101 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    20161 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    20221 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    20281 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    20341 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    20401 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    20461 acttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    20521 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    20581 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    20641 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    20701 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    20761 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    20821 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    20881 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    20941 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    21001 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    21061 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    21121 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    21181 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    21241 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    21301 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    21361 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    21421 cttcgagcga gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    21481 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt cggagaagat
    21541 aactcgacag tccaatacgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    21601 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    21661 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaatt aacaggtgtt
    21721 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    21781 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    21841 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    21901 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    21961 ttgccagtga cttatctatc tctaggcgga tacatgcttg gattgatact aggagcttca
    22021 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    22081 atgccgattc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    22141 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    22201 gtactcgttt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    22261 aggaatgaac gaaaaaaagc ataaaacaat cctaaaatac agaaggaagc tggacatcta
    22321 ttgttcccag cttctttttg tatagaccta ttttatttat tttactatat tggatatata
    22381 ttcattgaaa atagtgtata acaactttat aatattgagt ataccaattt ttggaggcgt
    22441 tttaatggac cgttatttag atttactttc agaaaaattc ccctcaacgg aggatgtcat
    22501 cacggaaatc atcaatctcg aagcaatttt gcagcttcca aaaggaacag agttattttt
    22561 gagtgatata catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa
    22621 tcttaaagaa aagatcaaag accttttttc tgaacaaatg acggaaacgg atatcagcca
    22681 attcactatt tttacggctt atccagaata tgcattaacg actgattggt acaaagaaca
    22741 agataaatcg cagctgatcc atcatttgat cgatctttta cggtttacga cagtcaaata
    22801 tacccggtca aaagttcgaa aaggattgcc gaaagaatat agctatatca ttgaggaact
    22861 cttgtatatc gatgaccgta tcaatggaaa aaaagactat gtaaaaaaaa tcattgaaca
    22921 acttgttttg atgaaggaag aagaaaaatt tttaacgaaa cttgcacaga cgatccaaaa
    22981 atctgtcatc gatcatttgc atatcgttgg agatattttt gaccgaggaa ctcaagcagc
    23041 caaagtgatg gatcgaatca tggatttttc ttctgtagat atccaatggg gaaatcatga
    23101 tattttgtgg attggtgcgt attgcggctc tgaggcatgt ttactgacac ttttacggat
    23161 tgcagcacgc tataattatt tatttgaatt agagaaagaa tatgggttga atttacgtcc
    23221 attgttttct tttgctcatc aaacttatca gaagaatcca gtatttgcgc ctaaaaacag
    23281 taaaaatcta tccgaaagag agcaagaaga attagaaaaa atccatcagg cactttctat
    23341 ccttcaattc aaatcagaac atcagttatt ggatcgtcga cctgaattta aaatggacga
    23401 tcgtaaattg ctggaaaaaa tcgactatga agcatcaacg attgatttag aaggtcagct
    23461 ctatgagttg aaaggaactt gttttcaaac gatccagcca gatgatccaa aaaaattctt
    23521 ggaagaggaa caaaaagtga ttgatagttt gatgtattca ttccagcact ctttgagaat
    23581 gcaaaaacat atgacgtttt tgatcgaaaa agggggaatg tatctcacga acaatcagca
    23641 tgtgctgttt catggctgta tacctgtaga cgagaaggga cagctcttag catttgaatt
    23701 agaccaatct tatcgcggaa aagaactgct gggctttttt gaaaaacaga taactgaaag
    23761 cgcaaaagat ctgactgcaa aagaagattt agcaaccgat ttgatttggt atgcgtggag
    23821 tgggccattt tcgcctttat tcggcaagag taaaatggcg acttttgaac gttattttct
    23881 cacagagcct catacgcacg tcgagaaaag caatccttat tatcacttgc gagatgaaga
    23941 gtggttcagt gaaaaaattt tagcagaatt cgacacaaaa gaagaagggt cagcaatcat
    24001 taatgggcac acgccagtaa aagtaaaaaa aggggagtca ccgatcaaag caaatggaaa
    24061 agtatttgtc attgacggag gtttatccaa agcttatcaa aaaacgacag gaattgccgg
    24121 ttattcacta ttatgcaatt cttatggttt ccaaatcgtg acacattatc cgtttttgcc
    24181 tattgaacaa caatttgcaa aaaaatccga ccaaacaaat gtgaaaaaag taatcgaaca
    24241 gcaactacca cgcaaactca atcgtgatac aacaaaaggt atggaactgc agcagcagat
    24301 tacccagctc aaaaggctgt tggactatcg aaaggacgat tgagaagaat tccagtgaac
    24361 attgttccat agactggaga ctgagacaaa agtcgtgtcg cagtctcttt ttccggataa
    24421 acggtgttct tctgccaact cctcggtcac aagtcacact attcaacaaa catgaaaagc
    24481 tgttttttga aattgctcct tgtgactcag agttgaacgg caggctcacg acctcttggt
    24541 ataaacggtg ttcaaaagcc agtttctcgg tattaagcca aatttatgca aaaatgtgaa
    24601 aagccatttt cacaaaattt ttcttaatac tcgaaactaa acagcttttt cacaaccccc
    24661 tcagaaagtc tattaaaatt cattcacaaa gaaatcgaat aagcgctgtt cagatggatg
    24721 tacacggtgg gtaagttccg gatgccattg aacaccgagg attttatgca tatcatcttt
    24781 gctttgaacc gcttcaacga gtccatcgtt agaaaaagcg atggctttta ataaaggtga
    24841 aagatctttg actgcctgat gatggtaaga attcacaaaa tatggatcgg ggagaaggtt
    24901 gcttaacaaa ctgtctgaaa caatcttgac ttcgtgagtc gtaaattgcg gggcagtggg
    24961 ctgttgttca tgttttactg accactttgg atataatgaa agatcttgat aaagtgtacc
    25021 ttccaaaacg acattcaata gttggagtcc ccggcagact gcaaaaattg gttttctttg
    25081 tttcaatgct tccaagatca gtgcctgttc aaattggtct cgttgaatat ttgtttccgt
    25141 tagctttgga tgaggatctt ccatgtaaag ttgtggactg acatcttgac cacctgcaag
    25201 aataagtttg tcgatttgtc caatcagttg aggagctgaa tctggagaca tgactggcag
    25261 aataagaggc aatccttgag catctaaaac tgcatccacg aatccttgcg gtgtatagct
    25321 cacttgattt ccttggaaag tatctgttgc tcgaatcagt tgatttccgg caatgccgat
    25381 gattggtttc atttgtattc cccctaaagt agacttattg taacaaggat ttaaaaaaaa
    25441 ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac tatctgtaaa
    25501 gagaaatttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt tgttataata
    25561 aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt cttcaagggg
    25621 tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag aggtacatac
    25681 tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt gaagcaagtg
    25741 ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgaaaagcg tgctcaaaca
    25801 ctgcggcagc agctgaagat ctatgcaaca gaaaaagagc aggaattgga aacgatccaa
    25861 acaaactatc gacaacaact aaaacagttg gtggaagaaa aagaacaatt tgaaatacaa
    25921 gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca agcaactttg
    25981 aaagatcaat atcaaaagca tcagagtcaa gcgatcggtg cgatcattga aagggtgaaa
    26041 gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata aaagaaatca
    26101 ggagaggatt ttacaagcag tacaaggaat ccaaaacgtt gaaatccggg atctgtatca
    26161 aaatagaaca aacaaccagt gggtggagaa gtattttcca gataccacga ttattgataa
    26221 agaagcaaaa ttaagcgctt taggtaatcg gttgaaagac atccaagaag ctatccaatt
    26281 catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc aagaactttc
    26341 cttacaagat ttggaagcca attattccga agaaaatttc ataaataaat tgaaagaagt
    26401 ccttgattta aaagaaaagt gggaacaact aactgaagaa cgtgatcgtt tagaagaaga
    26461 ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt atgcaagtaa
    26521 gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt tcaaggaaga
    26581 actgagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa aacaagttta
    26641 tctttcgtat gttgctttaa aaaaatcagt tgctgttata gaagaaattg ctgggaaata
    26701 cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaac tgcaagagac
    26761 caagcagcaa ttatccattg ttcacgagca gcaaaagaag ataacgacag cgttaggtca
    26821 gtgcagtggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg ccgttgcaga
    26881 acgagaaaaa ataaaagaac ggtttatcca tgcgacttat ctgatcgttt tgcaaggttg
    26941 ggtagatgca gaagaaaaac agaatttgct gcatgtgtta aacgaaaccg tatcagaaga
    27001 agatatatac gtcagtttcg aaacaccaaa ctcagaagag attcagcagg aagtccctac
    27061 taaattgaaa aaccatccgc ttgtcgaacc ttttgaattg ctgactgaaa tgtacagtct
    27121 gccaaaatat gaagaagtag atccaacacc ttggatgaca ccattttatc ttgtcttctt
    27181 cgggatgatg gtcgcagatg ttggttatgg tttgctgatg ctcattggga caatactagc
    27241 tcaaaaacta cttgttctac cacgcgggat gaaacgtttc gtaaaattct ttgagattct
    27301 ctcgatacca tcgatcatct ggggattgat ctacagttca tttttcggac agtcattacc
    27361 aaaagaactg tttggcatcc gcttgccttt cccaatccta tctacgacgg atgacgtcaa
    27421 tacgattttg attttatccg tgattttcgg tctgatacag attttggttg gattatttgt
    27481 ggcagcaaag gaaaatatca ggagaaaaga ttacttgagt gctgtcagtg atggatttgc
    27541 ctggcaaggg atcctaattg ggatcgttat tgcgttggtt ggtgccatgt tgataaaaaa
    27601 tacagcattt gtttaccttg gcgctgggct atcgatcatt gctgccttat gtatcttgat
    27661 cgttccgatt atccaaacgc cgtctaaagt caaaggtgct gccaaaggtg cgtataatct
    27721 ttacggactg accggctata tcggtgattt agtcagttat acacgtttga tggcattggg
    27781 tatttcaggc ggaagtattg gagcggcatt caatatgcta gttgctttca tgccgccagc
    27841 tgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga atatgttttt
    27901 gacactgcta agtgcctatg tccatggcgc acgattgcag tatgtcgaat tttttgggaa
    27961 gttttataca ggaggcggac gtgcctttga tccgctgaaa acggcggaaa aatacgtcaa
    28021 catcaatcac aagaaaaaaa acaataaata attggaggat ttttatataa tgatggatta
    28081 cttaattagt caaaatggtg gaatggtatt tgcggtatta gcgatggcaa cagcgacgat
    28141 tttttcaggg atcggttcag ctaaaggtgt tggaatgaca ggtgaggcag cagcagcatt
    28201 gacaacaagt caacccgaaa aattcgggca ggctctgatt ctgcaattgc ttccaggcac
    28261 gcaaggtttg tatggattcg ttattgcctt tttgattttt atcaacttag gaaatgacat
    28321 gtcagttgta caaggactga actttctagg tgcttctctg ccgattgctt ttacaggctt
    28381 gttttcaggt attgcccaag gaaaagtcgc agcggcaggt attcaaattt tagcgaaaaa
    28441 acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat acgcgatttt
    28501 aggtttcgtt atttccttcc tattagtgtt gaatgcctaa gcataaagga ggaaaagcag
    28561 atgaacgcca ttgaaaaaat catctctcag atgaacgaag cagcagagca ggaaagagcg
    28621 gcgttagaac aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa acgtacgcaa
    28681 gttgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattactcga aaaaaaatac
    28741 cgtcagctaa ggaatcgcca gcaagtagaa gtacgacaag aaaatttgaa tgcgaagcag
    28801 gaatttttaa gacgattgtt tgcagatgcg gtaactgaaa tggaaaattg ggatgaatct
    28861 gaacaaatcc agtttatcaa aaatgcattg tattcattac ctttgacagg aaaagtagca
    28921 ttcattgctg gagaaaaatc agcagcctat ctttctcaga cgttgttgga tgaatggaac
    28981 aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca agcgggtttt
    29041 ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt acaagatatc
    29101 caaggaacga tgagttttga gattgccaat caattattcg aataaggagg gatcagtgta
    29161 tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt ttgtccaatg
    29221 agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa atattgaaat
    29281 ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa cagaatcttt
    29341 taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag cctgaaatcg
    29401 tttggatcta tacgatgcgt tatactttcc ataatctgaa ggttttgaca aaagctgaga
    29461 tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca ttagaaacct
    29521 tgaaggatgc cattcatacg caagtttcaa atgaacttcc tgaaagcatt atggacagta
    29581 tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt gacgtgattt
    29641 atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga tatcaagaat
    29701 tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg gctcgaggaa
    29761 tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg ggaagtatcg
    29821 aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg aattttctat
    29881 tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat attgatctcg
    29941 taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa gcacaaaccc
    30001 aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta gaaagcaaaa
    30061 accttcgttt actggttatc ggcaaaagaa gccatttcga atcagaaaca atcagaaagc
    30121 gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga ttctgttttg
    30181 ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac cgttcgtcag
    30241 gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga gcaatgtgca
    30301 gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc tatcgtattg
    30361 attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa aaacgtagaa
    30421 aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt gcaaattgga
    30481 aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga tgccagtatc
    30541 caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga gatgcgtggc
    30601 gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg cgaaccggtt
    30661 cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc acaaatgttt
    30721 gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa tttcttaggg
    30781 cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga accaacagta
    30841 gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga aacaaaagtt
    30901 ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac aatcgaaaaa
    30961 ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg tgacaaaaat
    31021 ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga aaaattaaac
    31081 ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc agtgacaaaa
    31141 ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt tcagcatcaa
    31201 attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga gcgtggaaat
    31261 gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac aggagaatcg
    31321 ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc agctcgagaa
    31381 gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg ttatgacgtt
    31441 gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat gagcggacgt
    31501 ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg gcttgcggaa
    31561 tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga aggaagtata
    31621 accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt cacacaaaat
    31681 acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca aaaaagacat
    31741 tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt agggaagtat
    31801 atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat gcgtattttg
    31861 caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc cctttcagat
    31921 aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt gcagcaaaat
    31981 gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat gttgaagatg
    32041 atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta tttaagtgag
    32101 atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata tatcccggaa
    32161 acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat gcaaacgatc
    32221 gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg aagtcgtcgg
    32281 tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa tcgaagtccg
    32341 tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag ataaagcaat
    32401 ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg tacgattttt
    32461 aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat tcgatggttt
    32521 gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg atattaacgg
    32581 agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa caggtatctc
    32641 tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat tttctggctc
    32701 tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg tactcgattc
    32761 atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag aagctgaatt
    32821 tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt ttatgaattt
    32881 agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga ctgctgcaga
    32941 atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca tgaccaacta
    33001 tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa gaagaggata
    33061 tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa gaatccgcgg
    33121 actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg ataaaactca
    33181 tccaatccct gacttgactg gttatattac agaaggacag atcatcttga ctagagagct
    33241 ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc ggttaaaaga
    33301 taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga atcagctttt
    33361 tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag gtgaatctgc
    33421 tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa aagaatatgt
    33481 caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag ggtgggaact
    33541 gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc tggacaaata
    33601 tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga cccgcatgga
    33661 attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat tattaaaaga
    33721 taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca accagctgcg
    33781 tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg caaatgcttc
    33841 gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg ttgatttgtc
    33901 tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg aatatgatga
    33961 acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg aactggatcg
    34021 gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag aaatcgaaaa
    34081 aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca atgcattaga
    34141 atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga agctggaaga
    34201 aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta ccactgacta
    34261 agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca aataaagaaa
    34321 cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt agttggcggc
    34381 ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt tatcgatgcg
    34441 cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa tacagcagag
    34501 cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg cggtcttggt
    34561 tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag tctcagcaca
    34621 cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat gaagctcatg
    34681 ctttacatcc taaaattggc ctttgtgatc caattgattg gtacggttgc tttatctttt
    34741 gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt ccatgcaatc
    34801 tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa tcaccagcaa
    34861 aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt aggcttcatc
    34921 gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca ttcaaaaatc
    34981 gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt tttcacagag
    35041 cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa cacctttttt
    35101 atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca tatgagccat
    35161 gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg gtcgacggcc
    35221 ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc gatgttgaaa
    35281 ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt gttgcgggca
    35341 cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat tttatcggtg
    35401 actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt cttctctgcg
    35461 tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat agggaaattg
    35521 atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt cttctctctg
    35581 atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag tgtcatgatt
    35641 ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa ataaaataat
    35701 ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg aggcactacc
    35761 cgttgttgaa cctcgggtag tgcctctttt ttattgaaag gaatcaataa gatttgattc
    35821 ctttatttat caataggtta taattcatat ttttttattt tttcttttac gatacttagt
    35881 ccagaagcta aaatttcaag gttattagag gatagggaaa ttcgaacgaa tgcatctggt
    35941 tgcgcacgtg tagtaaagcg tctggaatga taaagtcgga taccgttagc aagaaagtca
    36001 ttctcaatta tcttagggtc aatctcagga tcaactggaa gaattcgata gtaagggaca
    36061 cgattcgttg gtttttctac tttgaaaaga gaatcaaaaa gctgattggc tctttttgta
    36121 agacttaatt tagtttctaa gatcctattt gcttcatttg aatggaggat ttgagtgatg
    36181 acttccacat ctaatccaga agttttcaca tttatattaa aaattgcttg ttctatcttg
    36241 ctttggacat gtactggaac tacaagataa gcaactcgca atccagaaca aataaatttt
    36301 gtcattccag ctaagtagat tgtttggtta ggtaataaat tttggaaagg aggaatgatc
    36361 gtatgttgat agtaagttgt taaaaatgaa tggatatcat cttcaataac ccatagacct
    36421 tctttttcaa tcacttcggc taattgctct cgtcttttgt gagagatttg aaaccctata
    36481 ggattattac aagaaggcat aagaaagaca ccatgaatct tcttttttct acattcctga
    36541 atcaatatat caggtcgcat tccttgctcg tcaaaatcaa ttggaacgat ctctaaatga
    36601 tataactgag ccaattcaat gaaattagcg tatgtatatc ggtctgttgc tattctatca
    36661 cctggagaga aaagtgcaga aagtgtgata gccaatccat tttgaacacc ggaaacaata
    36721 gatagagatt ctaaggacgg aatgactcct tgagttttta accaatttgc tgcagtagtc
    36781 aattgaaaat ttgtgccttt gggatcttgg taattaagca aaccaacaag atttgggtta
    36841 ttactagttg attgaataaa cgggacaacg aatttattac attcttcaaa agaactaacc
    36901 agaccaaaat caatgatatg ttcggttact tcattggtag agatggtgat ggactcaaag
    36961 acattgggag aaataaatgt gccacttcca atattcgtat acagcaaacc tttctcaatt
    37021 ccatatttat aagccttgcc aacggtagta aaatttaaat ctaaataatc agctagctct
    37081 ctttgggagg gtaactgagt gtttttctgt aatttattct ttaaaatatc ttgttctaat
    37141 tgttgaatca acgatcgata ataaggacgc ttcaattttt gtttgtctgg tcgccaagtt
    37201 aacgggtaat tgtcataaga attaataggc attttctcca ctccttaaaa ttgtatacca
    37261 tacaatttta ccattgattg tattgatttt agaagttaat cttttgtgag gagggaattg
    37321 tctatgaaaa ttattaattt tgaggaagta gtagcttctt ttagttttga agaagcgatt
    37381 caagtaatga aaaaatgttt ttttgattat gaaagtggta aaatcagcca aaatcctcga
    37441 ttggtggaaa ttttacctga cggaaaaggc aaaaatatgt ttgcgatgat gcctgcttat
    37501 ttgggggaga acagatattt tggcacaaaa gtcattaccg cttttccgga taatcatcag
    37561 tatcatttac cttctcattt aggagagatt ttcttgtttg atgctcaaaa cggactaccc
    37621 aaagcattga taaacgcaaa tgcagtcacc tggattcgaa cagctgctgt atctgcgctg
    37681 gcaacagatt acttagcaaa tccgcatgca gaaacacttg ctttaattgg agctggtcaa
    37741 caagcattgt cgcatttgac tgcgatttct accattcgtc ccatcactac agtgtttgtt
    37801 tacgatattt tagaagaaag acgaaatact tttatcgcta aagctaaaaa acagtatcct
    37861 aaaataacgt ttataaatgc aatatcagta gaagatgcag tacgtccagc ggaaatcatt
    37921 tgtacgttga cctctagtaa agaggcattt cttcaaaaag agtgggtaag ggaagacgca
    37981 catatcaatg ctgtgggcac atttactcct gatacaagag agataagtac atcgttgatg
    38041 gaaacttctc aaatctatgt agatgattac tcagctgcct taacagaaag cggggacctg
    38101 cttatagcca tggcagaagg agatttagag gaatcttgta ttgttggtac attaggcgaa
    38161 ttagtttctg agaaaaaaac attttataaa ggagaatcga ggccgacgat tttcgacgca
    38221 gttggtctag ctattgaaga tctttgttgt gcagaatata tttataacaa attagagaaa
    38281 ggggctttaa aatgaactta gattattacg tagttgatgc atttgcagat gaggtgttca
    38341 aaggaaatcc agcagcggtt tatgtgctag aagaatggct gccagaaggt acgatgcaaa
    38401 aaattgccat tgaaaacaat ttatctgaaa cagcatttac agtgaagaaa aaccaagaat
    38461 ttgagcttag atggtttacg ccagatcgag aaatcgatct ttgtggtcac gcgacattag
    38521 caacagcatt tgttttattt aactattaca aaatacctga tgaaacgatt aaattttcta
    38581 ctcaaagcgg taatctattt gtgacaagaa ttaaagatta ttattacatg gattttccta
    38641 gtattatgcc gaaaaaagtg ccgatattgt ctgaatatga agaagcgatt ggggctaaga
    38701 ttaaagaagc ctacttagca cgtgatttat ttttcgtatt agaagatgaa aaaacggtgg
    38761 ctaaattgca gccagatttt acagctatca aaaattttga attaggaata ggagtcatcg
    38821 tgacagcaga aagtgtgcaa aaagactttg tttctagaac cttctttcca aaattaacaa
    38881 tcaatgaaga tccagtatgt ggatcagcac attctaatct tattccttat tgggcagaaa
    38941 gaatgaataa aacaaaattg tcagcttatc aaatgtcatc aaggggcggg gatctagaat
    39001 gtgaattaaa agaagatcgt gtgataatag gaggaaaagc agttttgttt tctaaaggta
    39061 cctcgtttat ttgatttctt tcaaatgaat taaaaataaa ttggtggaga gtggcaacct
    39121 tatagctaca atggttgtta aaaaaagacg caaacttttt caatttaatg aaaagtttgc
    39181 gtctcatttt aattattggt gtttgttttt tattcaatca agagtaaaat gccccatact
    39241 aactatcctc ggatgacttc gattttataa ccatcagggt caatgacaaa gtagtaagaa
    39301 ggcgcagtgc caggtaatcc ttttaagtca gtaatctcaa gaccagcagc tttatgttgc
    39361 tcgtgtaatt tttctagatc atcagctgct atagcgatat gaccatagcc gtctcctaga
    39421 tcatacgcac catgatcata gttgtaagtc aactctaatt catagtcatc cccaggaagt
    39481 gtcaaataaa caagtgtaaa tttgttttct ggaaaatcac ggcggcggct ttcttcaaaa
    39541 ccaaatgcct tttgataaaa ttcgattgat gcttcaagat ctttcactcg aacacatgtg
    39601 tgagccattt tcatagattc atctctcctt ttccttttac tcaatcataa caaaaagaag
    39661 taaggaaata gttgatttgt gcttgttcat gagaaatttc tcatgtaaaa tggatagaaa
    39721 ataaaactgt taaggtggaa actattcatg gataaaccaa tttttggtga aaaaaagcaa
    39781 tttgctgatt atcaaacccg ttatgcagca tatattgtca tagaaagaga tcagcagatt
    39841 gcagtaatcg aggctccaaa tggtgctttt tttcttcctg gcggggaaat tgaaggagaa
    39901 gagacgaaag aagaagcgat tgtccgtgag ctgatagaag aaatgggaat tgtggcagaa
    39961 atttcttctt atctgggaga agctgatgag tatttttact cgaattatcg tgcaacttat
    40021 tattataatc caggatattt ctttgttgct gattcttgga agcgtatagg agaaccaact
    40081 gaaaagacca ataaaatttg gtgggtgagc cctcaagaag cgcttgtgaa attgaaacgt
    40141 ggtagccacc gatgggcagt cgagagatgg atcgaaaaaa ataaaaactg aaagaaagcg
    40201 agtagatcag ctttctctca gtttttttat ttattacagt acatattttt tgattgcttc
    40261 agctacaccg tgctcgtcgt ttgatgaagt gatgacattt gctgcttctt tgacaagaga
    40321 aacggcattt cccattgcta ctcctagacc agcatattca atcatggata agtcgttctc
    40381 gttatctccg atagccatga tttcatcttg atcaatacca aggtgctgtg cgagattcgc
    40441 aactgccgca cctttacttg cttctttatt tagtacttcg aagtaatagg gcgcactttt
    40501 gaccgttgtg tatttatcac ggaaagtttg cggtaaacgc gcaattgctg catctaaaat
    40561 ttctggttcg tcgatcatca tcattttgac gatgttcatg tctggtgtca tttcttcagg
    40621 tgttcgatat tttagcggca tcttgactaa acttgcttcg tggatcgtat aagcgctgat
    40681 atctcgatta ggcgtgtaga tcgctttgtc atcgatcgaa tggagatgac tgccgacacg
    40741 gcgtgccatt acttcgatct ctaggaaatc ttcatgtgtc agtccgtgac gggcgatgat
    40801 tttacctgat ttcgtttgtt gtacgagtgc cccgttataa gtaataacgt aatcattatc
    40861 ttgaaaaagg ttcaactcgt ttaactgttc ctggacacct ggtaagggac ggccagtgca
    40921 taaaacgatc gaaacacctt ttgcaacagc agcggcaatc gcttctttta cttcttttgt
    40981 tacttctctt ttctcattta ataaagtacc atcgatgtcg atagcgacta gtttgataga
    41041 cataatttcc tccaatatat tttaatttat ttcaatcaat gcgccattac ggatatggct
    41101 ggcaaattct tgataagttt cgtcaaataa atcataatga tcacgcaagg acggatcaac
    41161 catttctttt ggaaagaaaa atcgctcatc tccttgttct tgtcccgcta atgcagcaac
    41221 aagctgacta acctgtgata attctatcaa agttccgtca tttcgcaaaa tctcgatttg
    41281 tgtgcgatgt ctgccttgtt ctgggcgata aaaatcataa ggtagatcgt agctagaatt
    41341 cacagcagtg taataaacag gattatatcc gaccttttct actaacaaag tgagttcttg
    41401 gactaatttt gaatcccggt tgccagaaaa agtagcagat ttcagtggtt tacgattcaa
    41461 aaaacgttta gctaaatcat ttaaaatagg atcagcagat tcagaccatt gagtaaagta
    41521 agtgcccaag accccatcat caagtttgag gtattcttct aaagtaaagc ttccgtcaag
    41581 aaatggcaga agtaattgag aatggagttc aaaactgctg gcatcttctt gatacaactc
    41641 gtgtgcgcga tgaagcagat gctccaaaat tacttccata ccacgggaaa caggatggaa
    41701 gtacacttgc acatacattt gatatcggct aacgatataa tcttccactg catgcatgcc
    41761 actcatggaa aaagcgatcc cgccttcata aggacgaatc acacgtaata ttcgggtaag
    41821 atcaaatgtt ccatattctg ttccagtgaa ataagcatcc cgaagcaaat agtccattcg
    41881 atctgcatcg atctgactag aaatcatttg gacaacttgt gggttggggt aagttttttg
    41941 gatcacacta gctacctttt caggaaaacc ttcttctact cggttaagaa tctgatacac
    42001 ttctgtttct ggagaagtga tgatctctac agtgatggct tcatgattcg tatggaagat
    42061 atgttcaaat gtatgagagt aaggaccatg accgacatca tgcaacagtg ccgcacataa
    42121 agtcaccagc cgttcctgat catcccagcc tccgttgcct attttttctt ttgaaaaatt
    42181 tcgagaaaag atatcgcaga ttcgtcttga aatctcataa acgcctaatg aatgagtaaa
    42241 acggctatgt tctgctccgt gaaaagtgaa agaagaagtg cctaattgtt taatgcgtcg
    42301 tagacgttgg acttctttcg aattgatcaa atctaagatt acttgatgct ggacatggat
    42361 atagttatgg actggatcac gaaaaacttt ttccataggc agcatttgat atttataagg
    42421 ggttgtcata atgtagctcc tttatgttat tacgttgttc cattctctgg atttggttgt
    42481 tccattgctc cagctggatt tcttttttta gccagtcggt agaatcctgt aaatcacttg
    42541 cagaatacag cttttcaaaa gcttttgcaa atgcttcttt gacttctttt acaaaaaggg
    42601 gttggtcaag cagttcttca agtgttgcca ttgtagcagg atccactgca ggatagccat
    42661 ttgtaccaaa gtcttctttt aaagcagatt gataaaatcg cttcatgact tgcccacgtt
    42721 tttcctgatt accgttgaca cttaagtaca tcataacaga aatcccattt ttgatccgac
    42781 gctgagcgat accggcaaat tttttgccat ggatgcttaa atcataagtt ccaggacaat
    42841 aagaatgggt gatttcgaat gcttgtatcg gtgcctttgg aaaagcgtat ttagtcaata
    42901 ggagcatttg ctcgtaagcg tcatctatgg ttattttttt tgtgtcactt ttggggaaaa
    42961 tcaaagaaat attcaaaatt ccttcatctg atacgatccc taatccacct gaatttctga
    43021 tcactggagc gtaacctgca tcttccagtg tttggatccc gtcacttaga aaaggagttc
    43081 gaacatcctt catacctaag atgactgtgc agtcatattg ccagaaatgt aaaaagattt
    43141 gctggtgttc tttagaatat tccgttaata tatctgttaa tgcaaaaggg gaaaagtcgt
    43201 ttttttgata aatgtgctga tctagtcgaa tcgcagggtt cattcaaccg gctcctttgt
    43261 ataaattcat attttctaca atattatact ctaaaatcaa agagttgttt tggaaaaaag
    43321 gttttctgta aaaatggaca agttggttga caaaacaaaa gaaactagga acaatgtagc
    43381 tatgagaagt aaaggagaat gaataatgga attatcacaa ggaacaccga tttcaatcaa
    43441 attgcgtaca aaagtaaaac agcaagggga agtgcaagat ttttattttg atttgaaagg
    43501 gcaaatggtg acaattggcg atacgctgta catacgttat aaagagattc aggaggagac
    43561 gggcgatgag attcctgtaa cgatcaaatt agttccagat ggtcatgtac agctgatccg
    43621 tgcaggagaa atgcggatgc gtttgaaatt tggctataaa gaacggttag aaacatccta
    43681 tcgaacacca tacggtatga tccagattgc tacttttacg aaagaactgc atgttagttt
    43741 gaaagaccgt cccacagcag ggaaggttcg aatcgattat gatctgttca tgggaccgga
    43801 aaaaatcggc gaatattatt tgaccttgga ttttacagcg taaatataaa agaaaagttt
    43861 gattttttgt gtctgatttt gtatgataag gagtagactg tgaaaggacg tgtcctgatt
    43921 ggaaattagt gtatttgaag gtgcaaacaa aagcgaatta tcaatgatcg aagtagcaca
    43981 cgcgatctta gaacaacgtg gcgatgtcat ggatttttct gatttagcta accagatcca
    44041 aaactatctt gggaaatcag acagtgaaat tcgtgattca ctcgcacaat tttatactga
    44101 cttgaatatt gacggcagct ttatctcatt aggggataat cgttggggct tgcgttcatg
    44161 gtatgctatc gattcgatcg atgaagaagt aaaccatgga atggacgaag aggatgaaga
    44221 tactccacgt cgccgtaaac gcaagaaagt caacgccttt atcaatgatg atgaagacgc
    44281 gatcgattac aatgacgatg acccagaaga tacagatctg acagaagaag atgacgatga
    44341 tctgtttgat gacgatgatg atgaagatga agaaatcgct gcttataatt ctgacttgca
    44401 agaaatcgga gcagatgacc cgacagatga tgaagaagac ttgccaggaa atatcgaaga
    44461 agatttaagt atcatagacg aagacgatga tgatgaagat tatgaagagg aagaattttc
    44521 tgactctgaa aaagaataaa cattattttc ttacgagagg ttgtgacaga gacgatcagc
    44581 ctatcagaat aaggaggtgt gacacgattt ttgtcacacc ttcttttttg tatataaaaa
    44641 tcccaagtaa atacataaaa aataaaaaaa ttgaaacttt tagcgcacct tgtacgtcat
    44701 attaagtgag gcagaaagga gtggagaatg atttttactg gatataagaa aaaacggcag
    44761 atcgaaaaat accgtcacct gctgaatgga ctcatcgaaa ctgattatca aaaaatgttc
    44821 cgtatcacga tgaattacgt acatgataaa gaagaggcct tagatgtgat gcaagatagt
    44881 tttcataaag ccctgaacta ttttgaaaaa gaaaaagaga tcgagcattt tagtgcatgg
    44941 ttttatcgga tattgatccg cacggcatta gatagttggc gcagacaaaa aaggacgcct
    45001 gtggacttat ttgaaattca agatatcccc caacagttac ctttagaaac ttccgttgca
    45061 gagctccata tgattttaga taaaatagac agtccggaaa aagagattct gatcctctac
    45121 ttttttgaag gatttcgttt gaaagaaatt gcgactatct tggatatgaa tgaaaacaca
    45181 gttaaaacga aaatgtaccg ctcattgaat gctttacgtc aaatcctaga atgaaggaga
    45241 gaagagtgcc atgtattccc aaaaagaaaa tttaaaaaaa ctaaagaaaa cttataaaga
    45301 acaagcaatt cccaaagggc ttaaagagga gatccgccag cgttttatta tcgaagagca
    45361 atcatttata aaagcaaagc ggagaaagag acgtctccaa gggttcggag tatctttggc
    45421 tatcgtaatg attacaacaa gtagccttct ttttaatagt caagttcgaa gctttgcaga
    45481 agatttaccc atcttaggat cagtcattga attgatctta ggcgaacgct ttactgatcg
    45541 atctgaaaaa atcgatattc aggtaccgaa gatcaataca caaaatgaga aagaaaataa
    45601 aacaattcat ggattaaatc aaaaatactt tcgcgaagga caagcagatt ttgaaaaagc
    45661 tgaaaaagaa tatggaaatt ttgaaacgga tcattaccaa gttttgggag attatcagaa
    45721 aatcgtagac gataaccggt ttctcgtgat tgaaaggcag atcacccaaa cagcagcaga
    45781 tagccatgtt gaaaaaagat acgacacaat cgacaagaaa aacagtgtcc agctctcact
    45841 tccactttta ttcaaagatg acacctatct atctgttttg acaaaagaag tcaaacggca
    45901 gatggccgaa caagtcaagg aagatccatc taagtactat tggactgagc aagatattca
    45961 agaagggacg atagaaaaac caacacttgt tacgcctaca cgaagttttt atttgaacaa
    46021 agagcatcaa ttagttttga ctttttctca atacgaaatt gctcctggtt atatgggtac
    46081 accagaattt gtcattccta agtccgtgac aaaaacgatt ttggcatcgg aagattattt
    46141 ggatcattag acgagaagta gttgcgacta gcagtttcaa ttttcaagta agtacaagta
    46201 aataataaga agagaaaaag agaaagaaga gatatagtga aaagaaatta tttagttatc
    46261 agtattattg ctttatccgt gcttttgttt ttcgaaatta cttcggtggc ctccttgatc
    46321 accgaagtaa aaggacaaac acactcttcg gagcattcgt cacaatcttt ca
//
LOCUS       DACCKU010000012        45882 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.012, whole genome shotgun sequence.
ACCESSION   DACCKU010000012 DACCKU010000000
VERSION     DACCKU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 45882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45882
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.012"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..312)
                     /locus_tag="IVN13_000608"
     CDS             complement(<1..312)
                     /locus_tag="IVN13_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011125.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YE"
     gene            434..1018
                     /locus_tag="IVN13_000609"
     CDS             434..1018
                     /locus_tag="IVN13_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011126.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            1029..1274
                     /locus_tag="IVN13_000610"
     CDS             1029..1274
                     /locus_tag="IVN13_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011127.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            1280..2002
                     /locus_tag="IVN13_000611"
     CDS             1280..2002
                     /locus_tag="IVN13_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011128.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            2133..2876
                     /locus_tag="IVN13_000612"
     CDS             2133..2876
                     /locus_tag="IVN13_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011129.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            2882..3247
                     /locus_tag="IVN13_000613"
     CDS             2882..3247
                     /locus_tag="IVN13_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011130.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            3263..3793
                     /locus_tag="IVN13_000614"
     CDS             3263..3793
                     /locus_tag="IVN13_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011131.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            3955..4185
                     /locus_tag="IVN13_000615"
     CDS             3955..4185
                     /locus_tag="IVN13_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011132.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVRLPVSELKK"
     gene            complement(4389..4868)
                     /locus_tag="IVN13_000616"
     CDS             complement(4389..4868)
                     /locus_tag="IVN13_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011133.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            4988..6325
                     /locus_tag="IVN13_000617"
     CDS             4988..6325
                     /locus_tag="IVN13_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP7011134.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6348..6875
                     /locus_tag="IVN13_000618"
     CDS             6348..6875
                     /locus_tag="IVN13_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011135.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            6862..7425
                     /locus_tag="IVN13_000619"
     CDS             6862..7425
                     /locus_tag="IVN13_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011136.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            7848..8585
                     /locus_tag="IVN13_000620"
     CDS             7848..8585
                     /locus_tag="IVN13_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011137.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8602..9018)
                     /locus_tag="IVN13_000621"
     CDS             complement(8602..9018)
                     /locus_tag="IVN13_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011138.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9410..10657
                     /locus_tag="IVN13_000622"
     CDS             9410..10657
                     /locus_tag="IVN13_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAP7011139.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            10927..13317
                     /locus_tag="IVN13_000623"
     CDS             10927..13317
                     /locus_tag="IVN13_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAP7011140.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEVTNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            13366..15201
                     /locus_tag="IVN13_000624"
     CDS             13366..15201
                     /locus_tag="IVN13_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7011141.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15276..16133
                     /locus_tag="IVN13_000625"
     CDS             15276..16133
                     /locus_tag="IVN13_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7011142.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            16123..17232
                     /locus_tag="IVN13_000626"
     CDS             16123..17232
                     /locus_tag="IVN13_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7011143.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            17571..18371
                     /locus_tag="IVN13_000627"
     CDS             17571..18371
                     /locus_tag="IVN13_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011144.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18459..19265
                     /locus_tag="IVN13_000628"
     CDS             18459..19265
                     /locus_tag="IVN13_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011145.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19497..20366
                     /locus_tag="IVN13_000629"
     CDS             19497..20366
                     /locus_tag="IVN13_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAP7011146.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            20386..20733
                     /locus_tag="IVN13_000630"
     CDS             20386..20733
                     /locus_tag="IVN13_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011147.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            20746..22008
                     /locus_tag="IVN13_000631"
     CDS             20746..22008
                     /locus_tag="IVN13_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011148.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            22088..23464
                     /locus_tag="IVN13_000632"
     CDS             22088..23464
                     /locus_tag="IVN13_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7011149.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTAMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMIIKLVNGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAESRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANSSSKEPSIEKKSTFALKLEKNKEEKEKQGAKTLEKLQAEKLEKVLKALDK
                     ADSEPQTSNNTNNEQPISSKTKPKTTDPKVSVNVTITTDKSEKKQVNGTTSPIMQKML
                     KAKKQVHK"
     gene            23461..24090
                     /locus_tag="IVN13_000633"
     CDS             23461..24090
                     /locus_tag="IVN13_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011150.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRTKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
     gene            24109..24588
                     /locus_tag="IVN13_000634"
     CDS             24109..24588
                     /locus_tag="IVN13_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011151.1"
                     /translation="MLELFEANRTGFNQKLEVGETIEYKDKNYIIIGIYNTRINRIGD
                     PRITSDLVCQSVNYSPDLTSRYKKYVLLEYRHKITDEIGVNNTRNIFKIGTVIPYSNN
                     EEKIFYQIYGIEKFEYEHVDLLVTYQMRLIEPWSQAEIDKAVKLNRLSKFNVLGNAF"
     gene            25090..25143
                     /locus_tag="IVN13_000635"
                     /pseudo
     CDS             25090..25143
                     /locus_tag="IVN13_000635"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25147..25746
                     /locus_tag="IVN13_000636"
     CDS             25147..25746
                     /locus_tag="IVN13_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP7011152.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            25762..26238
                     /locus_tag="IVN13_000637"
     CDS             25762..26238
                     /locus_tag="IVN13_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011153.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSEDSKSMRDLLITYLNEHEFEQTKVKKKNKK"
     gene            26255..27157
                     /locus_tag="IVN13_000638"
     CDS             26255..27157
                     /locus_tag="IVN13_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011154.1"
                     /translation="MLFTLLGYLCLVLAFFGAIAIFVGQFQENNPIMRNIGIGIVIIS
                     LIVFGATKIFGSNDQQFGISGVDNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDDK
                     KASDDVLATIKPSQSQFEVFYTVKENNPVRKLTLVFNVDGEEYYKDVKINKASTKKIV
                     ESAKKEVATEESTTESSSTVEVELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQRINVTLFNAVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGSDLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            27230..27424
                     /locus_tag="IVN13_000639"
     CDS             27230..27424
                     /locus_tag="IVN13_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011155.1"
                     /translation="MVVNKEKVRKYVTLKIASVEYLEKRAKEEEKTRGKKVTVSELLE
                     ELIESDQTIRRAFTNRSVKS"
     gene            27529..27957
                     /locus_tag="IVN13_000640"
     CDS             27529..27957
                     /locus_tag="IVN13_000640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011156.1"
                     /translation="MIIQKFDWKMKLGISLVILGFIIFLGIKAQKNQSTFETMSSQII
                     EECSKIYYCENKTEESNGFYYIDQPHDVVIKMESHEVNEWIQRSTNVSKKLERYSQTF
                     LSKVIFSNCKNEETSQRVASSLENLKFSVSKKEKFVDLRK"
     gene            28192..28779
                     /locus_tag="IVN13_000641"
     CDS             28192..28779
                     /locus_tag="IVN13_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011157.1"
                     /translation="MENNNDIRYFSIGERVTTVSELSKDYRDKEGTIISLKKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNDGSHSIEKAILVFPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITNKEAKVEDVLTVRELQSLIEYRNKQITNPEYLKKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDNFKKIYLKEIEKD"
     gene            28779..29678
                     /locus_tag="IVN13_000642"
     CDS             28779..29678
                     /locus_tag="IVN13_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAP7011158.1"
                     /translation="MWSVTLPFQPVSAPRPSARRNVADATNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQGLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            29678..30592
                     /locus_tag="IVN13_000643"
     CDS             29678..30592
                     /locus_tag="IVN13_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011159.1"
                     /translation="MNNNEKILHVLDSFETIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKKAMELNEDRRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPANSGIGIVLKAIDEEEIEDFIPAEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCEGYEVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     DARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNASNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKEAE"
     gene            30644..30859
                     /locus_tag="IVN13_000644"
     CDS             30644..30859
                     /locus_tag="IVN13_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011160.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLQQFWLNNFSYKDFIKEKANRLY"
     gene            31049..31303
                     /locus_tag="IVN13_000645"
     CDS             31049..31303
                     /locus_tag="IVN13_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3034 family protein"
                     /protein_id="HAP7011161.1"
                     /translation="MKKEELIDMFQIVERANNMGIMFFDRISLKMDLSVAHQEFNLRL
                     KALLISDDVNFAHDVVGIQNHIDRENKRMGDGFLPRYSSL"
     gene            31482..32531
                     /locus_tag="IVN13_000646"
     CDS             31482..32531
                     /locus_tag="IVN13_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAP7011162.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGSPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     APNPTPAPAPSNEKVLNVPVIWQGNTMLCEGASLLEGLHYKGVATNQNLTSFVNYMPR
                     ANDNNPYHGYSGEWRYNVNGTYQGMMPDPVVKWAKNVGGNAVNITGSGTNGIKNEIRN
                     GNPVVTWITYNYASPQFKQMPWGRAVWNGHVVLVDGFKNGAYHVVDPVFGAKWINAST
                     YERAFNVTGMAVAVR"
     gene            32708..32938
                     /locus_tag="IVN13_000647"
     CDS             32708..32938
                     /locus_tag="IVN13_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011163.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEEEYKENIPENLQGS
                     ERYEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            32941..33198
                     /locus_tag="IVN13_000648"
     CDS             32941..33198
                     /locus_tag="IVN13_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011164.1"
                     /translation="MINEDFRSEQKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            33415..34290
                     /locus_tag="IVN13_000649"
     CDS             33415..34290
                     /locus_tag="IVN13_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011165.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            34300..35094
                     /locus_tag="IVN13_000650"
     CDS             34300..35094
                     /locus_tag="IVN13_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011166.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            35123..36241
                     /locus_tag="IVN13_000651"
     CDS             35123..36241
                     /locus_tag="IVN13_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011167.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLKTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            36274..36594
                     /locus_tag="IVN13_000652"
     CDS             36274..36594
                     /locus_tag="IVN13_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011168.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            36849..37538
                     /locus_tag="IVN13_000653"
     CDS             36849..37538
                     /locus_tag="IVN13_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HAP7011169.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            37539..37742
                     /locus_tag="IVN13_000654"
     CDS             37539..37742
                     /locus_tag="IVN13_000654"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011170.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            37762..39390
                     /locus_tag="IVN13_000655"
     CDS             37762..39390
                     /locus_tag="IVN13_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011171.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            39441..39812
                     /locus_tag="IVN13_000656"
     CDS             39441..39812
                     /locus_tag="IVN13_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011172.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            39897..40097
                     /locus_tag="IVN13_000657"
     CDS             39897..40097
                     /locus_tag="IVN13_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011173.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            40231..41457
                     /locus_tag="IVN13_000658"
     CDS             40231..41457
                     /locus_tag="IVN13_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011174.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            42307..43632
                     /locus_tag="IVN13_000659"
     CDS             42307..43632
                     /locus_tag="IVN13_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7011175.1"
                     /translation="MFDYSREPVNDYFLIDMKSFYASVECIERNLDPMTTPLIVMSRS
                     DNTGSGLTLASSPTAKKKYHITNVSRATDLPYPLPKELHIVPPRMNLYIKKNMEVNQI
                     FRKYVADEDLLIYSIDESILKVTKSLNLFTSSGTRSERRRELAKIIKDEIQKETGLIA
                     TVGIGDNPLLAKLALDNEAKSSKEFIAEWTYETVPEKIWKINEMTDFWGIGSRMKKRL
                     NHMGIMSIEDLANWNPYTIKSQLGVIGLQLYFHTNGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPKDYTRREEVELVVKEMAEQVAIRIRQHNCKTGCVRLAIGTSILEEKSGFSHQMKI
                     PITDNTKELQNYCLFLFNKFYEGQEVRHIGVTYSRLVYTDSLQLDLFSDPRKKIAEEN
                     LDRIIDKIRQKYGFTAIIHASSMLEGARSITRSTLIGGHAGGLGGLHND"
     gene            43625..43972
                     /locus_tag="IVN13_000660"
     CDS             43625..43972
                     /locus_tag="IVN13_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011176.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLRWSQI"
     gene            44323..45144
                     /locus_tag="IVN13_000661"
     CDS             44323..45144
                     /locus_tag="IVN13_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7011177.1"
                     /translation="MGLLVPRANTYTIPLTKRQLIAKRYQRITRAINREFWNSESDTA
                     HSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQVIKNAFQEILPN
                     SDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMGGNWKATNPKKEQ
                     EAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEAMGNWKFVENNSG
                     GQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICLEKVLKKIAL"
     gene            45164..45724
                     /locus_tag="IVN13_000662"
     CDS             45164..45724
                     /locus_tag="IVN13_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HAP7011178.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
BASE COUNT        17432 a         6151 c         9112 g        13187 t
ORIGIN      
        1 ctcatattca tagctatcaa ttagctcagt tgaactataa gctttttcta gtagttccat
       61 ttcccatttt ggagagtttg gatcactttt tcttagtcta gctatatctt ctaaaatttt
      121 cttagaagta ttatcctcgt aaatatttgc taaatcagat aatttatcaa ttaaatctaa
      181 aataagcgca ctgtaattga cagctattgc agaacgataa tttccctgat aaaaactact
      241 tctaacttct tctaataatg gaataattct aggattttta acttttttta tttcatcatc
      301 taatataaac atataaatct ccttttttta tttgtatgac aagtttacca taaaaaaaaa
      361 ttaaaaagcg agacatattt atgttgttgt gttatcataa taacataaat atgtagagtg
      421 agatgatgaa aaaatggatt tgaaacaatc gttttacatc tggttatatc ctgataatcc
      481 tatgtcaata atggctgata tttttgtagt tagcatctta ttgtatttat tttatctaat
      541 tttctttcac aatttaatcg ggtacatgat tcttgattgg aaaggaatgt tcgatataga
      601 aagaaaacca tttgctaaca atcaaaccta taccgtcttc gaaagtggag attgggaaga
      661 aaatgaagtt gatgtgatcc atgaaattgg attagcttta tcaggaatac aagatgctga
      721 tgttttggta tcaatgacaa cggatactgg atacaacgct tatgaggtat tttatcttat
      781 gtattttata tggcgtgatg gtcaagaaat tatcaatggt cgtgctgaaa aaacaatcaa
      841 ttctaatact gaaatttctt tttgtatggg tgaaggggaa atgattgtat ataaagatgg
      901 gtcagcaaac attaaaacag cctttggtga tattgaaaca agtattaagg aacttaggga
      961 atatgtgcag aacccatata attttcaata tttagcagga agaataaatt tagtttagga
     1021 gtaaaaatat gaaaaaaatt ataaatcaac caacaaatca cttgataatt gaaggtgtta
     1081 ttttcgtaac aatgtatttt actgtaggaa atggcatctc aaatacaatc attcaaaaaa
     1141 atccacaata cctattattt agtctttatg cttggattgt acaaatggtt tgggtttcca
     1201 tattgatgct gttgttttat tggatagtac gggttattct tttattgtta aacaaaagaa
     1261 aaaaggatag gtgaataaaa tgaaatcaac taaattgaag gttgtattgt tatcgacagc
     1321 agcattaata acccttacag catgtggtac aactaatagt gctaataata acaaagacac
     1381 aaagtctagt agtacgatgg ttactaaaaa cagcactaca tcggcatcaa caacaatgtc
     1441 ttcaatgact gaaaataatg ctgaaaagta tcaaaaatac gaagacatta ataaaggtca
     1501 aaagccggat gaagtttata acaactttat taatacagta aaaaatgatg aaaattatgg
     1561 gaatgatcca ttgcaaaata aaaatctgac tatcgttctt tacaaacctg attgtccaga
     1621 ttgtcaaaaa gcagaatcaa aaatcatgca agaaataaaa gaaggattta gttatgctcc
     1681 aacattaatg catgatatta ccataaataa accagcttac gagtattacg cagttgctgt
     1741 agatattact gatggtgtac ctgagtgggt acaaaacatt cctggtctgg ctgatgagaa
     1801 caatcaatat catacgccaa cagttgcagt agtaacacca aagaaagtta acggcacact
     1861 atactgggat gtaatgggaa tgacaacaaa tacaactgat gaaatgattg aaagtacaac
     1921 taaatattat atggatttgg gagcatataa attatatcaa caatcagagc ctgttatgga
     1981 cgatcaatcc aaagttaaat agatctttag ggcgtaagcc ctattttttt aaaattaagt
     2041 gataacaaat aaatgatttc tgaaacaaac gttcttactc aactactcga aacacacaag
     2101 ctaattaact agattataaa ggagaatgcg taatgaaaga aatcagtgca aatgaagctc
     2161 atattccgca aaagaatcag gttggtatat ataaggtgct tcatggtgaa ggaatttata
     2221 aaatacagtt acttaccggt tacaataatg gtgcaaatgg gaagtggtta aaggcaacaa
     2281 gtattcgtga acttagaagt gaagtagaaa aaattaggct tggtctcaca agtgaagaaa
     2341 tagctaaact gaagggaaat aattcttttg taattgaaaa agaaaaggaa aaacatctac
     2401 tttatcgtga gctgaaaatt gaagaatact atgaaattga tttacctgaa aaattaagaa
     2461 ttattgatca acgttcagaa gaaaaatgga aaactgaaat atatccaagc ttaaatgact
     2521 acaagacatc aggaaagccc atgtataact acacaggctt tttaacctac acacaataca
     2581 aatatgcctt tcgcaaatta aaaggcatta aacaagctga aattgctctg tatgagatga
     2641 aaaaagtgga tagattgaaa ttgattcaag atttagtgca aaatacatgg aatcaagcga
     2701 ttgtcgagct agaaaaagaa aatgggaaaa ttttaactaa taaatatctg atcgtaaatg
     2761 aaattgaaga attaattggt ttattttcat gtgaaaaagg tgaagtcttc ttgaagtcat
     2821 caccaaccta ttcaattttg gacagtccgt atcagtttat acaggcaagg agatgagtta
     2881 gatgtctagc ttgaaagatg actatatcca agcaaaacaa gagtatgaaa aaatacagat
     2941 gttgaaatct gagaaagtcg aattattttt aacacaaaaa ttagcagagg atcatattgc
     3001 taacttagat gctaaaactt tagaggaata tgatcggcag tttaatcaga tacatcacaa
     3061 tatcattttg cttgttgagc aggcaaagcg aaggtttgaa aaagcggaat atgatctatt
     3121 agatcaagga attgtatgct tggataaggg attgcgaaga tattatggtg cagagttaaa
     3181 aaagcaaaat gtagatagaa atgcactaaa acatgaaatc ttagaattga taatgcaacg
     3241 aatttgaagg gatagaaagc aaatgtcaca aaacattagt gctgtaaaac aattaataga
     3301 agaagattta gagaccacag ttcagagatt agcggaacga atccattttg caaattatca
     3361 aaccatctct aactggtcaa gcaggaacag aaatccggaa gaaattccaa tgaaattttt
     3421 ttatcgaatc ggaaaagaat atggtgttga tccgataaaa attttcgaga agaccatgat
     3481 ttattactat catggaacac ctaacgaagt gttagaacat cttttggaag aggcaaaaga
     3541 aaaatcatca atattagagc tatcgcaaaa tgacaaagtt attcaagtga aatgcgttga
     3601 tccaacagga aagattattg tcggttttga tattgaaaga aaatccaaaa tagattttat
     3661 tacacaatta aaaaagataa ataatttaga tattctaacg cttagaccag atcatgaaca
     3721 gttgtttatg agttttaaaa aacaattgaa agaaatacaa aaaagatata acaaggtgtt
     3781 tcctaatgcg tgaaaagctc aaagaaatca gagttttttg gataattaac aaagaatgta
     3841 tcatcttata tactcttgga tttcttagca tgatcttgtt ttggtggtta tatttaaaac
     3901 agtaattgga gagggtcaag aggattttta tcaagataag gatgtggaaa ctgcatgtgg
     3961 aataatagat taaaaacagg attgttatta atagttattt cctgcgctat gatgattggt
     4021 atgaggatac aaagggaaca atcctatttt gaagtttctg ctaacaacgt aatagaaaaa
     4081 tgctattacg gtcaacatta ttggagtgag gaagtcagag aaaatataga tcgtgaatat
     4141 gttcaaagaa ttgttagatt accagtaagt gaactaaaaa aatgatgaga ttatcagcat
     4201 atcttttaat gtttttgaat gtggtcacgt taatctttgt agtaatcggt gtgactttcg
     4261 atttaatgga atagtgggaa atctataatg gaaaaaagag acacgaatga acatattttt
     4321 tgaatcaata ttgttgatgg tttttgcgat cagcgcttac cagaaatcta aaaaagagta
     4381 atgattgttt aatcatcact ctttttttgt agggaaattg aaatatgtgg agcttgtatt
     4441 ggtttagtta ggattgcttg gctataagga gctagttctt tatcaaaacc atcaaagtag
     4501 gttttcagta gcgatttaac tgatcgctca tcttgtttga tttggataat ttcagcatcc
     4561 aaatcggttg tgagatcagg agcttttgat gcgattacct gatttttttt atcttccaac
     4621 tctcttaggc tgtttaacaa gttgtaaaaa gaatcatttt gattgttcat tccaacgtga
     4681 gttgcaattt taacagaagt ttcgtccgta ttgtcatcaa aagaactcat tggaatatca
     4741 agtagtaacg aggatgtttc ttttactgac tttttcttta cagcattttt tgtttttcca
     4801 tcttttttgc tctttactcg agatgttttt ggagctagtt taggtaaatc acttgtttta
     4861 tcagtcattt attagctttt cctttctatt aatttatgtt attatattat cataataaca
     4921 taaaaaagta cactttttgg gggcgtagtt attgaaaaaa tctaagagaa agattgccac
     4981 attgcttatg ttaatggtgc aatttacagg gattgcaatg agtgtaatca caataaatcc
     5041 gcaagtgata catgctgatg ggatagctac ttatgatgca caatttaact gtactactga
     5101 aggtagtggg tcaggaacag atagcgatgg aaacgatact ggaagtggcg ctgtaacagg
     5161 tgatgtagcc aaaaatattg aagatattta caaatacatg aatggagaat atggcttatc
     5221 aggtgctagt attgcaggta ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg
     5281 agcagtagaa gggtattacg gagatggaca gagagaagtt gacttcatca aacaatttac
     5341 tgatggtctg atgggtattg gctatggtca atggacagct gggcgacata cagcattagt
     5401 tgattggtct aagaaaaata atggcggttc ttggtggact acagaagccc aattggactt
     5461 tatgttcaaa ggtgatgggg gattcgtagg aatattaaaa gattatgctt taaatgcttc
     5521 tgatgatgtc gtagaaaatg cagtaacttt tcataaaaac tgggaaattt cagctgattc
     5581 cgaatcagct gtaatagctg tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa
     5641 aagcaagggt atggacggtg caaaagatga aagtaagatc aataaaatat ccggaggttc
     5701 aagtaccgga gatggatcga ataattcatc aagttcagca tcaggaacag tagaagatgc
     5761 ctgtgcaact acggatgacg ctaattctgg ttctggatca attgggaaag aaggaaaaat
     5821 taacggtaaa aaaggtcaag taatagcgaa tatgacttat gaagaagctt tgaaaaaata
     5881 tgggaaagcg atcacgctac caaaattcaa agaaccagat tggtcaactt caccttttaa
     5941 cggaatatat caaggtcagt gtacagagtt gacgtgggct tatatgagcc aattatacga
     6001 tgggaatcaa ccgactactg gaaatggtgg ttatgtatgg gaaagctaca aatcggcagg
     6061 agcaaaaata acgaacaaac caatcgtagg ttatgggttt agtgcttcag atggatggtt
     6121 atgggctgtt ggcgatgcag ggcatactgg tgtagttata gcggtgtttg aagatgggtc
     6181 atttctatgt gctaacttta atgttccacc aacccaagct ccaacaaggg gagtttcggt
     6241 aactttgatt gatggagtgg acggatcaga taatgtgcat ttctttagcg gtgtaggaaa
     6301 agctaaattt gaaatcgaaa aataaattgg aatgaaggac gatatagatg aatagtaaag
     6361 tgaaagatac aattatcatt tctttgcttt ctggtatgtt attagcactg cttgttggaa
     6421 tcctattctt caataagcag aatcaggaca tagaagctga aaaacacagt gtgcatattg
     6481 aagataagct gacattcttt aaagaggtta aaccaaatga ggtggatgga ttacggaaag
     6541 atttagaaaa tgatattcaa gaaatgttga atggatcagt tcaaaataag tttgcagatc
     6601 aatcaggtga agaatttttg agaaaactat ttattgtaac aggtaaacca ccaattactg
     6661 ataagagtag tgagaaagaa aaaatcgagt actataagcc ttttactttt aagctaacaa
     6721 actttggaat taagacatct agtagtgggt tgtatgaagc attgaccgat tttgaagttc
     6781 aatacaatgg aaaaaaagtg tatgatacca cattaaccat cacactaaat aatgataaga
     6841 aagtgactgg gggtgctttt tatgcaaaat catgaaatca ctactgctaa aaaggcgatc
     6901 ggaatacttt ccataattgt gatcctttgt tgtgtggtac tatttcaaaa gaaaaatgct
     6961 tatgataata atgtcaaaga attggaagac cttaaaagta aacaaatcga agtagtcaag
     7021 aataaccaga aagtgaaagc tatgcaggaa gctcaataca aagaattggg gaatgctgaa
     7081 atcaaggaac aaagtaggaa attctttaat ttattttaca actggaattc atgggaaaaa
     7141 tatacgaaga atatgaagga aatacaaaaa gaatttcctc agatagtaaa gtctagtcct
     7201 gttgatattt ctggcacgat ggtaggcact ggtaatagtc caatatctag ctatactgct
     7261 gattactata caacaaatca aaaaggtaaa atggttgaat tgattacaca gacaagaaat
     7321 actccaacaa cagcaacaac aacaatttgg cgtggagtaa caagcatagg agaaaatcaa
     7381 ttgttttact tggaggattt agatagctat gtaagagcgg agtaaaataa aaaaagagct
     7441 aacggtacca acgttagctc tttgtgctga cgatgataac aaaacctacc gaaaaaggat
     7501 tgtttcatca caaacacaaa cagaaaagaa ttgataaaaa acccgaacct cagaattttt
     7561 attaatcaat tcatttagat tataataaat tgagctgaac attacaatac gtatatgttg
     7621 taaaacgtaa aaaaagtcaa aagtaaatac tttcttaaaa agtcaaataa aactattaaa
     7681 gacaatatat atggtttatt tgacataaga aaatggtcta gatgataatt gtaatataga
     7741 attgtaggct taaataaagc ataaaatacc ttttatgata aggtatagct cttaatttta
     7801 agcagttttt agatataaga gtgaaagtaa agtgggggag aataataatg gcagatcgta
     7861 aattagaaaa gttattagaa gaaacgtgga atccaaaaga atttagtgaa ttttttatgg
     7921 agaattttga aactgactta gcagtgattg ttaaagatgc tcttagagag caagggtatc
     7981 ctgaaactgc aaactatata aacattaatt ttactttgta tactgaaaat aaaggaactt
     8041 gggatttttg ggctacttta gcaaataagg agttaagtga taaatcagac acaggaattc
     8101 gtaacttttt tgaatcaaac agagatgatt atatgtatgc taaccatcaa gataaattga
     8161 actttagagt agagtttgat gaaacacctg aagaatttat agaaagacaa ccgcctaaag
     8221 aaaatgtagc aaaggtattg gaagatcgtt ggaattcaga tgaaatagtt tcaacaatta
     8281 gcgaactagg cggacaatat gaaccgctag tagaagcggt tagagaagag ctaagactga
     8341 ataagtttcc tgatgttcaa aatattgatg tttctcaaat tgaaataaac gtcaagatca
     8401 cgaataaact tgattatggt agttgggcag atatagcatt agaaaaatac atctactcaa
     8461 cgttaaaaga gtttatcgaa aatagaatgg atatcatgta tctacagcat ccacagtatt
     8521 taaactttgg tgttgaaata gctacacctc ttgaagaatg gaaaatggag caagggcttg
     8581 actagccttt gctccatttt tttattttac aaatgaatac agttcgtcgt ttttaccaag
     8641 aatgattgtt tgcccaactt tgagattgtc aggtgatcct tcagcggttt gtaaggagaa
     8701 cgttccgttg aacttatcgg ataccgttat aggtagcttt aattctgatc cttcctcatc
     8761 tttgagaatt gctgtttttc gttcatcaaa ataaacagtc tttattaaat aatttttcgt
     8821 agtgatctca gcagatttgt ctttttttgt agtagtaaca gtttctttag aattagaggt
     8881 attttctacg actattccat ctttggcatt tgagcttgtg tttatttgat tgaatatttt
     8941 cagcgacaag ataccgctaa gaatagtcaa tatagaaaca agtgtaatta atactgataa
     9001 tttccttttt ttgttcattt tttcttaact tcctttttgt tttgattagt tagatattcc
     9061 ttatctaaca agccttttta ggctattaaa tgaagataaa ccccaaaaaa atattgcaat
     9121 tatgacagtg caatggtact attattatac aataaatttt gcgaaagggg taggtctaaa
     9181 tcgcaaacaa aaagaaattg aacctagaca aatacaactt gtacaccgca agggaagcaa
     9241 gtttgatttt gggtaaaggt agaaattatg ttacaaacat aatcaactat tatccaaatc
     9301 gtttgcctaa aggttcgttc aagaaagtgg gtgacaaaac ctatattata acggaagaag
     9361 gaatagaagc attgcgatca caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa
     9421 gaaaaattcg cttggtatgg ttttatttgc atcagctgta cttgcggggt tatcagtacc
     9481 agtaacctca catgctgatg aatggactgc aaacacaccg gatcaaatcc aaattacaac
     9541 aggacaatca agttatgcgt tcaaaaaagg agatactatt tggtcaactg tagagaagct
     9601 taaaagctta ggttacaaga ctactgttgc tgagtttgcc gaattgaaca atgttgattt
     9661 ggcaaaagga gaacaacgga ctatcccaat tggacgaact ttcaacatca gtaaagatac
     9721 attaacagtt aaggaagcag atggcgaagt agttagtcaa actgttcaaa aagataaacc
     9781 tgatcctgtt gaaaatacgg agcaagagaa caaaaaatcc gagactgcaa aagaatcgga
     9841 accaacagca aacacttctg ataagacaga aacaagtgct tcaagtgttg atggagcaag
     9901 ttcatctaat acaaataatt ctacaagtga gaaagaagca tcacagggaa caagtgaaag
     9961 tagcactacg gaaactgata ctggaacatc aacaaataac tcacaatcaa catcaaatga
    10021 tgcaaatgaa tcaaccagta catcttctag tgatcaatca caaacaggtt cgagtgatga
    10081 aactgacagt tctacttcac caagtaaacc cgaacaacca actaatgttg tgctaggcgt
    10141 tccatttatt agtcaaggta acacaatgct ttgcgaagga acatctttgc tagaagcgtt
    10201 acattataaa ggtgttgcaa caaatcaaga tttaatgact tttgttaaat caatgccact
    10261 atctcctgat aataatccaa ataatggttt ttctggtgaa tggagacaca atgtggatgg
    10321 aacttatcaa ggtatgaatc cggctcctgt agtttcatgg ggtgttgcta atggcggaaa
    10381 tgttgtagat atttctggtt catcggtaca aggattaaaa gatgagattg taaatggaaa
    10441 tcctgtagta gcgtgggtaa cttatgcctt tgaacctgct caacataaat caatgttttg
    10501 gggcgatgct atttggaatc gacatgtagt agccgttgat ggatttaaag acggttttta
    10561 ccatgttgtc gatcctgtgt ttggggcaag ttggattaat gcaggatcat ttgaaacagc
    10621 atataacgta tctcaattag cggtttcggt acgttaataa aagagattgt ttatatacta
    10681 agtagttaag tatagtcttt atttgtcctt gcttaactac ttagaaaact tgttgctcat
    10741 ggtgggtaga actaaaaggg gtgatgctat gaatactaga gcgttgaaac agcttgcttt
    10801 caacgaaaaa agtgattaat aggtgtggtg attttaactt aggaatgggc gcgaaggtca
    10861 ttaccacacc gacatatcgt ggctcatttt cttcgcctaa ctggtataaa gaaggagtaa
    10921 cgagagatga agcatgaaaa aacgataatt ttaaaatcaa aaataattta ttgttttgcg
    10981 actttagtaa tggtgttatc tacgcttata atgccttttt cagcaataag agctgaagca
    11041 gatgtcacaa aaccgacaag tgcaaaattt acagaaactg gcgtaaaaac agacggttat
    11101 attagggtta atatgccgaa tcatccaaat gaatggatga tttcaagtca atttaaagat
    11161 tctcatggaa atattggata ttgtatggat tcagaattgc catccccaac aggaagtgga
    11221 gccggaagct tgaagtacaa gggagctggc agtgatgagt tctatcgtat gtttaaaggt
    11281 ggttttccat caaaaacagc aaaggaactg ggtgcaggaa atgatacaga agcatggtat
    11341 gcaacacaat tagtttcatg ggttttagcc ggaaacttta aagtttcaca gattgtatgg
    11401 tcacatccaa accataccgc cgcagaaaca gctcgtgtaa aaaaagcatt tgaaaaaatt
    11461 tatgactatg ctaagaatgg aaaagatacg cccaacactg agttttctat cacagcttct
    11521 aagacagctg acgaaggaaa atatcacact ttcacatata aaacagcatc aaataagact
    11581 ggtaatgcga agctcacttt tacatcagca aaaccagctg gtatgaagat ttatgatgca
    11641 gatggtaaag aagttactaa caacactgtg aaattaaatt caagttttac tattaaagta
    11701 cctgtgacta caccaagtgg tactttgtca tttaaaggta ctgctaatgt tagcacaacc
    11761 aatcctttta cttttgatgg acgaggcgtg tatcaagatg ccgtagttat gatcactact
    11821 tcagaaacaa aggactctaa atctttgagt gctaaatgga caagagctgt tggtcaggta
    11881 acagttaaga aagttgatca agatggaaat gctttaaaag gagcggagtt cactctaact
    11941 gacaataaca atgctaaatc tgttaagaca accggagatg atgggttagc tacgtttgat
    12001 ttgcaacaag gcttaacata cagcttagaa gaaacaaaag ttcctgaagg ttatcaaggt
    12061 tcatttaaaa aggatggtat tactgttaat accgatggac aaaaatttag ttacactgct
    12121 acgaacacaa ttaaaaaagg taaagtaaaa gttaaaaagg ttgaccaaga tggaaaacca
    12181 ttagctaatg ctgaatttac cttgacggat gattcatccc aaacacaagc agaaaaaaca
    12241 gatgaaaacg gacttgcaag cttcgatatt gtagcaaaca agacctacag ccttgaagag
    12301 accggagtac caactggtta tacaggcgca ttcaagcaag aaggaattac cgttaaagat
    12361 gatggacaaa cttttgaata tacagctaca aacactttga aaaaaggtgt agtaaaagtt
    12421 cataaagtcg atcaagatgg aaaacctttg gcaaatgcag agttcacttt aaccgatgaa
    12481 aaaggtgcaa cacaagtaga aaaaacagat gaaaatggac ttgcaagctt caatattgta
    12541 gcaaacaaga cgtatagtct agaagaaacc ggagtaccag taggttacac aggtgcattt
    12601 aagcaagagg gtatcagtgt taaagacgat gggcaaactt ttgaatatac agctacaaat
    12661 aatttgaaaa aaggtgtagt aaaagttcat aaagtcgatc aagatggaaa acctttagca
    12721 gatgcagaat tcaccttaac ggatgaaaaa ggtgtaaaag aagttaaaaa gacggatgca
    12781 actggtctgg ctacgttcaa cattattgcg aacaaaactt acaacttaga agaaacaaaa
    12841 gtaccaacag gttacacagg cgcattcaaa caagaaggaa ttaccgtcaa agatgatgga
    12901 caagtctttg aatatacagc taaaaacact ttgaaaaaag gtgtgatcaa agttcataaa
    12961 gtcgatgatc aagggaaagc attagcaaac gcagaattca ctttgacgga cgacaaaggt
    13021 gtaaaagttg tacaaaaaac ggacagaaat ggcttggcaa catttaatat tgttgccaac
    13081 aaaacgtaca aaatcgaaga aacacaaaac cctaatggtt atacagggaa atggtctaaa
    13141 gaaggcatca ccgttgctga cgatggacaa acctttgaat atacagtaat taatcacctt
    13201 gtacctgaaa cagtaagaac aggcggaatc ggtacatcag ccttccttct tgtatttcta
    13261 attgcaggat tgagtatggt gctactgtat tatcgagaac agcacaaagc aaaataaaaa
    13321 agtgttaatg gaaaagaagt aaatatgaaa aaagaaggct gacgtatgaa atcaaaaaaa
    13381 attttttcaa atttaatgct atttggtact ttgctaggag tgggattggg cgcaagctca
    13441 atctcttcta gccttggact aggggggcaa gtagcatatg cacaacaaaa ctataattat
    13501 caaggaactg gaacgattca tatccataaa gtaacaaata ctaatgggga tgggaaccct
    13561 gtctcagata ttgataacaa agggaaagaa atagctttac caaatggtgt aaaacctctt
    13621 gaaggcgttg agttctctta tgtgaaactt actaaagatg aagctaaatc cttgggatgg
    13681 acggatagtg atactacaaa aggtcaacat attgatgatg ctaaagcacg attattacta
    13741 aacaaaaaat acagtgaagt aagtagaatg aagactgatt cacaagggaa tatagacata
    13801 cctatacaag taaatacaga cgatttaaca gataacttgt atttgattgt tgaaacagga
    13861 acagtcaaag gtgtagttga agaagcgtat cctatgattg ttaacgtacc tgaactaaac
    13921 ggatcagatg atcaatactt taattatgat gtacatgtct atcctaaaaa ctatttccaa
    13981 ttaaactcag cagagcttga taaaaaagac gaagatggaa aggctcttgc cggaactgtt
    14041 tttgaattgc gaaaagatga tggatctgat caggttgttg gaacttataa aacagacagc
    14101 acaggtaaat taactgtgga tggtttggaa ttaggtgact attatttcct agaaaccgat
    14161 ttgggaacag atgctaatag aacaacctat ttactagatg gtaaaaaatt caaatttagc
    14221 gttaaacgtg gggagactgc taaagtagtt cttgatgtta ccaactacaa acgacctgta
    14281 attactaagg taattgataa agaaaccgtt gatactggcg aagatgttca atatacattg
    14341 aaaccatcaa ttcctggtaa tattggtaat tacgaaaaat atgttgtcaa agatgttcta
    14401 ggtgcagagt taacttatga ttcatcagaa gtgatcgaag gaatgacttt agatactgat
    14461 tacagtatcg aacgtgacgg acaaacgaca aactatgtct ttactgaatc aggtatggat
    14521 aagcttcaga aacaatttga tgcaggggat cgcttcttta caattttgat gaacgctcat
    14581 actaatagta aagctaaaat ggatacacca cttaagaatg atgtttcaac gatctacaac
    14641 aatggatatg aacccgaaga cgaaacacca gttgatcacg cttatacaat cattggtgat
    14701 caccaattca ttaaaacaga cggagataca agcaaagcat tgagtggagc aaaatttgta
    14761 gtgaagaaca aaattgatcc gaataaagtt acttacctaa aacaagatcc aacaacgaaa
    14821 gctgttactt ggattgcaga taaatctaaa gctactgaat tcgtttccgg atcagacggt
    14881 aagttccaaa ttgaaggttt gcaatatacc tatgatgtag attttgataa acaaggtaat
    14941 ccaacaatca cagataaaaa aatcaatgat tatgctcttg aagaagttgt tgctccttca
    15001 ggatatacac caactagaaa cgatgttcca tttgtggtat cgaatgatga tgcttctagc
    15061 gaagtaacag acatcaagaa ccttaaaaca cctgaaacag ttcgtacagg tggaattgga
    15121 acagtagtat ttctagtggt tgcaggtgtg gcaggaacag ggctatatgt tgtctacaaa
    15181 cgagaacaac acaaagctta atgaaataaa attccctaga gtgtttttgg ctctagggtt
    15241 tctttttaaa aagtgtgtga aaagtaggta tgagcatgaa aaagaagaat atcaagttga
    15301 tcatcttaaa aattgggatg ttgctatgtt taggagtagg tctatatcct gtaattactc
    15361 aggcttatac ctactaccaa actcaacaaa cactaaactc ctataaggaa aaatatgatg
    15421 aagcttctga aaaggagaat gaagaagtct tcaagagata ttcagaagtt aataggaaac
    15481 gaaaagacac agaacaaacc tcagtcacga ttcctgaaga taataacaat attccgtcaa
    15541 atggaaatct tcagaaggat aaattaggtg aattagtggg tgaaattctt ataccaaaga
    15601 ttaatgtgga tttgccaatt tatgatggtg taaacgattt ccaactatca gcaggagcag
    15661 gaatattaac cggaactgat tatccaactg gtgggattgg aaaccattct gttatatcag
    15721 ctcattcagg cattcctaat aaaaaatatt ttacagattt agagttgctc aaacttggtg
    15781 ataaattttt tattgatatt tcagggaaaa aacttgctta tcaagtagat cggataaaaa
    15841 caattctacc aaccgaattt gaggatttgc ttcctgtggc aggaaaagat tatgtaacac
    15901 ttatgacatg tacacctgtt ggagataatt ctcataggtt actcgttaga ggggttagca
    15961 taccttacca aaaaagtatt gttgagaaag aaaagaagaa agaagattta aaggtaaagg
    16021 cgcagatgaa ctggtatcct atcgtagttg taggggtttt attaataata gctgtgatag
    16081 tcattattaa aaaaatagta gcaaagagac gaggaatgaa aaatgaagaa taaaatacta
    16141 ctttttttga ctcttatttg cactttggtt ttaacaattt atcctgcaaa tcagctctat
    16201 catcaattca aaacaaaaga gaatgtggcg gaaatccaag agaaaattga gaaaaaacaa
    16261 actccttcaa aagaggaagt ggcaaaaaaa gtcgaacaaa taaaatcgaa cagcggtaac
    16321 caaggattag acaatactca gaaaacagat aaaggagtag acaatactca ggaaacagac
    16381 ggtggatcat ttgatattcg tgaagctgaa ggaaaggtca atccatacac aggagatgtt
    16441 ttttcaaaaa atcaaacgaa agattttgaa ccatctacct attcaggagc ggttggttat
    16501 gtctatattc caaaaataaa tgtgctagag cttttgtttc taggcgcttc acaggagcat
    16561 ttatatagag gtgtggcaca aatgagtgct actagtctac ctttatcagg taaaggtcat
    16621 caaacagtaa tagcaggtca tcgtggcggt tatacgtcac cacaatttct ttatgttgat
    16681 caattagtag ccggagacca catttatata gattatcttg gtgaaaaagc agtttatacg
    16741 gttttccgaa cagacgttat tagaaattac ggtcagaatt caacactgac tgatattcct
    16801 gatttagata tgttaacact ttatacttgt catccatatc ctcagaacta cgatcgttta
    16861 ttagttagaa gtctaagaga tgaaaaagca aaatacatca cgccacaagt aacagttggc
    16921 aataccgcat acgctgatgc cattgatagt gaatccgttt cagataagcc agaaactgtt
    16981 acatcttcta atgatttatt tgaggatgtt gatcgtgcca acaaggaatt agatggaaat
    17041 aatgatctta atattccgga acaaggtagc gtagagatgc aagacagaac gtttgctgaa
    17101 aaggcaagtt caatatttac aggaattacc aataaacatc agaaaatgct tttagtcatt
    17161 tggactgtat taggagcatt agcaagtcta aaggttctta ttcttttaat caaagagttt
    17221 agaaaagcat aatgaagatt ataaaaattg atagagtaca gtaaaaaggg attgatacta
    17281 ttattgatct cttttttatt ttgaatagag ctagattcac ttttaaataa aataaaaact
    17341 gatcttaatt ccaaagaaaa agtcaagaaa aaaatacaac aaattttttt atcagaatat
    17401 ttatcagaat attattagaa aagataaact caaaagctta atttaatagg gttattggtt
    17461 gtatttagaa ataatgtatg gaaaagctat aaagcaatag ataagatgta gaaaatctta
    17521 tttgtgaatg aaaaacaagt catcgtaaaa tattaatagg aggtgtagta atggtggaaa
    17581 tatcaaaaac agctttagac tcattagcta aactatttag tagcaggttt gatggtgcag
    17641 aatttgttga atatctaagc caaaacgaaa gtggaaaaac tctgatcgaa acactgaaaa
    17701 aagactattt gaatgaaaat gactttccga catggacgaa tattgaagat gctacatggg
    17761 atgtagtgtg tacgatatat tctccttggg taacgattgc tgattttgtt ttagataaaa
    17821 ttggagggga tacataccct gatgccaaat ctcttgctac aagtgacttt aaaacgttct
    17881 atggttatct aatgggtcta ataggcaatg gaatagattt gaaagttacg ccaaaacaaa
    17941 cacctgaaga aattgaaaaa gaaaaaggag aggttcaaat ggcttatgca gatgacagag
    18001 aaaaagtaaa attattggaa ggtaaattat ctgaagaaga acttggaaaa atggtgcaag
    18061 aaatcaatga agacagcaac tattttcctg aattgaaggt atatcaaaac gacgaagatt
    18121 tctttaatga aatgtttagt aatagtccat gggaagcagt gagtaaaaca aatttaggag
    18181 aatggaactt tcaagatgat ctagttgcat ttagcaatga tgggtattta cgatcatatt
    18241 cagaaagtga gtatagagct gaattaaaaa cgtatgaaac ggatattctg atgagctatt
    18301 taaatctatt agagagtggt cagggagagt taagagataa agttgaagaa ctattaaaag
    18361 aagagctata gggattattt caacataaac acaagcagaa aactaccaaa caaaacgtac
    18421 catgcaaaaa gccggttaaa ccggcttttt gctctattat gagaggagat caaaccatga
    18481 gtgcaacaac cgatatggct ttagaagcag taataaaaga taatacagga aatgtcgaaa
    18541 atgtagaaga ctttagcgat attgcaatca atctttcagc atggatttca gctgtaggag
    18601 aagaaaatat cgaatttgag atcaagaata tggatgactt aaaagcaaaa tttcctttta
    18661 tcgcatcgaa actaagtcaa tttgccgagg agaaatcgtg gaatgaatta gttagttttt
    18721 ccgaaaaaat ttatgatatt acgcaagaag atgagtgtgt ttcatgggaa atgaataatc
    18781 aatacaagaa aattgatgct ttcgtcaaat cacgtataga aggaacagct tttgagtttg
    18841 aaggtataga taccgcagat aattggttaa ataaagaatc ctttattaaa agtatagcgg
    18901 atgatcttga aaaacaatgg tctaaggaag actatgcaga gtacctaaaa acagaaagag
    18961 atacagaggt gaaagaaaaa ctcactgagg tatgtttgga tgaacatatt gatcccaata
    19021 tcgtaaaaca agaagactat gaagtggtta ttgataaagt tcctactgat tttgaggaag
    19081 ttgtaaagta tatagttgaa gaagcattat cgcaatactc ttcagatgag gaagtggacg
    19141 aagaaactgt tcgtaccaga gtttggcaac tggtttttga tgacaaagat tataaatatt
    19201 tgagtgatca aaactcggtt aattggagtg tacgattatt aattgatcaa aatgatattg
    19261 aataaaatag agctgaaaag ctctattttt tttgataaaa aaactagcta tttttttaaa
    19321 aataatttgg ttcaaaattt atttttttaa cttgataatt ttttgccatc cttttttaaa
    19381 ttggctatat atcaaggttt atagtgggga taaataaaaa tacatttgac aaaatttaca
    19441 atatagttta ttatatattt atcttattat gataacacaa taaaaggaat gatggtatga
    19501 aaaagaagaa gtttagtcga tcgttgttag gaattttagt tttagttgcg gttggtctta
    19561 gtggcaaggg ttactttcag aataacagca ctcaggaaca tgattttcag atcaaaagta
    19621 gtgttcaggt aggtaataaa gcagacagtg aatcaaaaga atttttaggg atacgttatg
    19681 atccaaacaa atatccaaat aatatcgtaa acattaatga tgggaaatat acactgacaa
    19741 aagaagacca tgaagcatta tcagaaaaag gcagtcaaac cttttgggta aattatgcag
    19801 atttagatca acttggaaga gcaggagatg ttgaagcgtt gatcacctat gatgctataa
    19861 attctcactc atctaaaaat gtggaacgtc catctttcaa aagttctgtc catattgcag
    19921 gagaatacaa ggatggagta tttgatcaag agagagatac ttgggtcggc aaagattcaa
    19981 acaatgaaat cactcagcta gaaggatata aaggttatct ctataacaaa agtcattcat
    20041 tagcatggtc gctaggagga gatatggagg tacacaattt aacacttgga acgagagcgc
    20101 aaaatgttgg gaaaaatgat cagaatggag ggatggctta ctcagaaacg attgtacgtg
    20161 atgcaatcta taaaaataag aatgaaaaag tattgtataa agttgatcca atttataaag
    20221 ggaatgaatt ggttcctcgt ggttctcatg tcgaagcttt ctcagttaac gataatggaa
    20281 aaacggtaaa ccttaatgtt tatgtattta acaatcaaaa aggaatcaac atcaactatc
    20341 aaaatggtac ttgggtaaga gattagaaat tgagaaggtg aagaattgga aacaataagc
    20401 atcaatggat tagataaatc agttgcaaat cgaataaaaa aaggaaatgg ctactatctt
    20461 ctgaagaagg gtaaatattc agttgctaga gagttagaaa caatgagtat cgtgtatgta
    20521 gctaagaatc attttgacgt agaacaggct atttttgatc cagattctga aatagggaaa
    20581 tgtgttgaat tatcagagaa aactgttagt acaaaagggg cgatgatcat tgagagagaa
    20641 atgttagacg aaatcagagt aatcattcct aaagatacgc agatgtgtga ggacagcata
    20701 agtgacgatg aaccaaaatc agaaagaaaa taaggagata ttcagatgag cgaaaaatcc
    20761 attgaattaa aggaatataa gcgaagcgat ggagaaaaat attatcctgt agccagtttt
    20821 tctaaatcag tatatctggt tggacgtgca ttggtagttg gtgcaattgg catatatatt
    20881 ttgcattggg taacaaaggt tgtacaactg cttgtagata ccttagtaag tattcttgtc
    20941 tcgttaaatg atgttaacgg aacagatgat ttaggattaa aagaaatgtt taaagagtta
    21001 ctcaatgcgt tttttcccaa ttatttaagt tattcatata atgtacaaag cttttttgca
    21061 aagctacact tggtagacat ctctatttta gtattagaag caggaacggt attctttgtt
    21121 ggatatttgg ttactttgat gttcaggcat cactatggag aacaaagacc attttttgat
    21181 gatatggaaa gtagaaggtt gaaaagagaa gctctagaag ctatggatgt gaagtatgat
    21241 ccaaacaaga ttataggaga tgaaacgaac aacaaaaaaa gaaaacgaaa caaaacgcaa
    21301 ttagaatccg atgtgctatc aacgcttaaa aatatcaaaa tttctgttca tacaagacgt
    21361 gatctaagtg tcaatgaaga tattcgagaa tatatcgtaa aaatcccgca accaaaaggg
    21421 aagaaagtta gagaaacagt tttacgacaa attcaagatt tagggtcgat cctgacaaga
    21481 gcaacaagcg gacgagccaa atttggtgaa aaagaagatg aagtttcagg cgattatttc
    21541 attttccgag catcagtaaa tattgaaaga gaacgtaagg aaagagtaag taccaaaaac
    21601 aaagaaacgg aaagagcaac atcttcaatc gcagtttcaa attcacaatt ttcttttcca
    21661 atcaatctct ttgccgataa ttccaaaaat atccaaaacc aaaccgaaaa agctagaaaa
    21721 tatgccgaaa ctcaaaagca atctttagaa ctttatctat cttctgctga cgtacctgca
    21781 agtctaacag atatgcacgt tgggaataca tctattgaat atttgtttac tttatctcgt
    21841 accagaaaaa ttcaactaag ctcaatgaaa gaagaaattg aaacttactt gcatatatca
    21901 ggtgtaatcg tgcggatcaa cgcagggcgt attcaaatta ctctaccatt aggagaaaat
    21961 gatcgtattc caattgatgt accatcaatg atcaatagcg tgttttaaaa gacatcacaa
    22021 atagaaacag aaaatacaaa ctaccagttc acaaacagaa aagtaccgag aaaagtgagg
    22081 aatagaaatg gctatgaaat tattagagaa agtcaaaaag agagccgaaa aagctgaatc
    22141 tattttgcta gataaagaac ctaatataaa gcttaatgga aaattcagtg tcaatatgtt
    22201 gaacacaaag gctaaagacc cgacagatgc tatttttgga attaagcctg atggcacaat
    22261 tgctcatttt tgcatcagcg aaatgccaca catgttggtt gcaggaacga caggatcagg
    22321 gaaatcagta ttactgaatg aaattcttgt tacagcaatg tgtcactcaa cgcctgatga
    22381 attaaaaata ggaattgtag acccaaaaag ggttgaattt ggaagataca aaaaattgcc
    22441 gttcatgttg gctgatccaa ttacagatat ggatgaagct tatgattttt ttgaatatct
    22501 tgtgatcttg atgcatgaac gttacaaact gatggaaaaa gcagggttac aaaacatcac
    22561 gctatataac gaatatgccg aaaaaaatgg attagataga tttccctatg tgatcttatt
    22621 agttgatgaa tattcacagt tggttggaac acataaagaa gtagaaggtt tgattgttca
    22681 attaggacaa atggcaaggg cagcaggtat tcatgttatc ttagctacac aatctcctcg
    22741 atcaacagtt gtcactggta ttatcaaagc caattttccg tcacgtgttt gccttatggt
    22801 agcaagtgat ttagaaagta ggatcgtatt agatgaaggc ggtggagaaa gcctaagtcc
    22861 aaagggagat atgattatta agctcgttaa tggaagtatg gttagagcgc agggagctta
    22921 catcagtaac aaagaaattg aaacaatttt caatcacctt agaaatacta tgccagaacc
    22981 agaatatgtt gattaccgag caatcgtagc tgaatctcgt ggtgagggtg gagaagacga
    23041 ggattttggt ggaattggta aagctcctgt aagaaaatct gctaaaagaa atttgaacac
    23101 acctttagca aattcatcat ctaaagagcc atcaattgaa aagaagagta cgtttgctct
    23161 aaaacttgaa aaaaacaaag aagaaaaaga aaaacaagga gctaaaacac tagaaaaact
    23221 tcaagccgaa aaattagaaa aagttttaaa agcattagat aaagctgatt ctgaaccgca
    23281 aacctcaaac aacacgaata atgaacagcc gatttcatct aaaactaaac cgaaaactac
    23341 tgatccgaaa gtttcagtaa acgtgaccat cacaaccgat aagtctgaga agaaacaagt
    23401 aaacggaacg acttcaccta tcatgcaaaa aatgttgaaa gccaaaaaac aggtgcataa
    23461 atgatcattg tcagttcttt tttgaggtgg cttacgccat ataaatatac agatgaagta
    23521 gttgtgaaac gaacgaagaa aagagataaa aaagcggtac taactcctaa acagatgctt
    23581 gatcatggca ttttaggggt gaaggtttta gaagtgaata aaccgtttga atcaaatagt
    23641 gcaagagaaa agttgcttgg agctgaagcc gatgtcgcat atttttacca tgacaaaagc
    23701 aagcatcaaa tagctattaa attcaatcca aaatcggcat ggcggtatca agcaagagat
    23761 gacttgaaac gtaggaaact tagaccaatg ctctatccag aaggaaaaga agcatttgat
    23821 agaacccaca tcgttcccat tggttttcat ggtacagaat cagataaccg cctattagta
    23881 ggttggaaca gcaatcaaaa tagagttcag atcaataaat ttgagaagaa agtaaggaaa
    23941 ttcaacgatc gaaaaacgat cctatggttt gcagatattt gtaaacagcc ggatggaaca
    24001 gcaaagtggg aagcggtgat atttgacgaa aatggtaaaa aagtaatgtc tgaaacatgg
    24061 catgataaag ctaaatttgt ttggcagtag cagtagaggt gaaaaagatt gctagaactt
    24121 tttgaagcaa atagaacagg ctttaatcaa aagttagaag tgggagaaac tatagaatat
    24181 aaagataaaa actatattat tatcggaatc tataacacga gaattaacag aataggtgat
    24241 ccacgtataa ccagtgactt ggtttgtcaa tctgttaact attcacctga tctgacaagc
    24301 aggtataaga aatacgtgtt attagaatat agacacaaga ttactgatga aattggtgta
    24361 aacaacacac gaaacatatt taagatcgga acagtgattc cttatagtaa caacgaagaa
    24421 aaaatatttt atcagattta cggaatagaa aagtttgaat atgaacatgt cgatctgcta
    24481 gtgacgtacc aaatgcgttt aatagaacct tggtcacagg cagaaattga taaagcagta
    24541 aaattgaacc gattatcaaa atttaacgta ttaggaaatg ccttttaaaa atcgcaatac
    24601 acaaacagaa aaataccaaa aaattagcaa tgaacgtagg taagaacatg acaataaata
    24661 ataataaccc taaaaaatat aatgctcttg tccaatgcta tatcgaaaac aaaattacaa
    24721 tgcaagaagt cgctgatgaa ttaggcaaat caaagagcct agtatcacac gattttaagc
    24781 acaatgtttt tccgaacccg attgagctga ggaaaaaatt tcaaaagaga gtttatcaag
    24841 aaataggtaa gtggattgat gaagggaatg caattgaccc tttaattgaa aaatatcctt
    24901 ttttaaaaag caaaaataaa attcaaaggt taattcgtca aggcgatatt caagctaaag
    24961 aacctgtttt gacaaccaat gatttggcta aaataaatct tcataaacaa attattgaag
    25021 actttgaaaa aaatctttca acaactgaaa ttttaaaaaa atacaatatt ccgagaggaa
    25081 agctaacatc gactcttttt agagaatatg gagaatcggc agttactaaa gcaaaggaag
    25141 tagcgaatga gagaaaaaaa caaaaatagt agtcgatacc tagtagcgac tgtagttttt
    25201 gtttgtgtat tgattttcct gtttgatttt ttgcagattg gaaaaagcat aattggaaat
    25261 acattgagtg caaaggaaat ggttgcttta ggtggtttgt atgaaggaca cttgttcccg
    25321 aatatattca tttctatcgt ttctcatatt aacttgtacc atttaatttc taatttattg
    25381 ttcttagtag ttcttgaaga agcactgaat gatcagtatg agtggttaac attattgatc
    25441 gttttaatca tatcaggatt tctaggaagc ttagttcctt ttctgctaca cagcaacatc
    25501 gtaagtatgg ggcttagtgg aggtattttt ggatggattg ctttatttgc gattgcatac
    25561 atccgttttg atagcaagaa acatttatta ctaagtgttt ttgtattatt gataatcggc
    25621 attttactgg tatctacttt tgaatcaggc gagacaaacg tcttgtcaca tgtaatcgga
    25681 tttgtctgtg ggatgatctt gggatttatt ctaaagaacg tttcattgat caaacaaaag
    25741 aattaaaagg gagtgctaaa aatgccaaaa aaaaaagaaa agaaaattcc agtgtttttg
    25801 tctgtctcag aaatgttatc acttcaaata ctgttaagaa aaaagattga aacaatagac
    25861 gaagaagtta gaaataaaag ggctatgtac gaacaacgtg aagcgctaag agaacgaatc
    25921 cactattttg atgatataca cactaaaatc tatgaagcac gtacaaatca cgaagacgaa
    25981 agcaaattag gattagctca acaacgaata tttgaatggc tgaatacgaa tactgagcaa
    26041 gaaccgttaa gagcaattag taatcttttt gaatttggaa cgaagcattg gtttaacgct
    26101 gtagaaaagg atgctgactt acaagaagcg ttcgatacac taagcgagga tagcaaatcc
    26161 atgcgagact tattgatcac ttatctaaat gagcatgaat ttgaacagac gaaagttaaa
    26221 aagaaaaata aaaaatagaa agttgtgatg tatcatgctg tttaccttat taggttattt
    26281 atgtttagtt cttgcttttt ttggagcaat tgctatattt gtcggtcagt ttcaagaaaa
    26341 taatccgata atgcgtaaca taggcattgg aattgttatc atatccttga ttgtatttgg
    26401 tgccacaaag atttttggga gtaatgatca gcagtttggt atttctggag ttgataatag
    26461 ttatgctcca gattcattgg gaagagtagt gatagatgga acgtttgaga atgtaacgag
    26521 ctacaaaaat gtttcattga cagtgaaaga agatgacaag aaagcatcag atgacgttct
    26581 cgctacgatc aagccgtctc aatcacagtt tgaagtgttt tatacagtca aagagaataa
    26641 ccctgtacgc aagctaacat tagtttttaa cgttgacgga gaagaatact ataaagatgt
    26701 aaaaatcaat aaagcatcaa cgaaaaagat tgtcgaatca gcgaaaaaag aagtagctac
    26761 agaagaaagt acaactgaat caagttctac tgtcgaagtt gaactaccag ataaaattga
    26821 ttttgatcct gaaaaagtca aatcgtttga atctgtttct ggttctatgg cagatcggtt
    26881 taacactgaa gttttcaaag ctatcaaaac tgttaaattg gatactgaaa agcaaaggat
    26941 taatgttacc ttattcaatg cagttgaatc tatggatcag gataaaaaag ttacggttgc
    27001 aagagaacta gggaaaagag taattggatt gtatcgaggt acggcaaaac cagattcaga
    27061 cggttcagat ttgccagttc acgtagttta tgaagacggt aaagtttttg gagactctac
    27121 agcaaatgaa gcactaacga ttaaattagc taaatagccg gaaaattata ataaacaagt
    27181 ggtgtcacac caccatcgca taggtgtgac accttggaga tgattactga tggtagtaaa
    27241 taaagaaaaa gtaagaaagt atgttacttt aaaaatagct tctgttgaat atttagaaaa
    27301 gcgagctaaa gaagaagaaa aaacaagagg taagaaggta acagtttcag agttgttaga
    27361 agaattaatt gagagtgacc aaacaataag acgtgcattt accaataggt ctgttaaaag
    27421 ttagggtgat actatcttta atttcaacca aatttctaat atatgatgaa tagtttcctt
    27481 gtacaaaata tctaattaag agataaaagt taggaggatt cattcaaaat gattatccaa
    27541 aagtttgact ggaaaatgaa attaggaatt agtcttgtaa tattgggatt tattattttt
    27601 ttggggataa aagcacaaaa aaatcaatct acttttgaaa ctatgtcttc tcaaattatt
    27661 gaggaatgtt ccaaaattta ttattgtgag aacaaaactg aagaatctaa tggcttttat
    27721 tatatcgatc aaccacatga tgttgttata aaaatggagt cacatgaagt aaacgaatgg
    27781 attcaaagaa gtactaatgt atcaaaaaag ttagaaagat attctcaaac gtttttaagt
    27841 aaagtcattt tttcaaattg caaaaatgaa gaaacgtctc aaagagtagc tagttcttta
    27901 gaaaatttaa aattttctgt ttcaaaaaaa gagaaatttg tagatttacg aaaataaact
    27961 atcgaaagta gtatgattat gatcttaaaa tatcatctta agagaagaat tacgattgtt
    28021 tataggattt acaaaatgaa taagtttgaa gagtgatttg gtcgcagact agatcactct
    28081 ttttgtgtaa aaaaacttta cacttatttt tgagtgtgtt aagatttttt atatagagtg
    28141 acggtttctt gtcagagtgg atgataacca tcaatattgg aggaataata agtggaaaac
    28201 aataatgata ttagatattt tagcattggg gaaagagtta caacagtaag tgaattatca
    28261 aaggattata gagataaaga aggaacgatt atttctctta aaaagaatcg aagaggtgtt
    28321 tggcaatatg ttgttgattt aggggatggc gttacagttg agtgtaaaga tagtcaactg
    28381 atcagaaatg atggcagtca ttccattgaa aaagcaattc ttgtattccc aaaagtatca
    28441 agcaagatta tgaatgactg gttgcttgga aaatttggtg agtacaataa aaaagaaatc
    28501 gcagaagtga tcggcaaatc ttatccaaca gtattagggt atctaaaaaa gataacgaac
    28561 aaagaagcga aagttgagga tgttttaaca gtaagagaac ttcagtcatt aatcgaatat
    28621 cggaataaac aaataacaaa tcctgagtac cttaaaaaag aagatggtct attgaaacaa
    28681 ttggaacagg taaataaaat ccggaaaatg caaaaggtag ctcctttaag cctagataac
    28741 tttaaaaaaa tctatttgaa agagatcgaa aaagactaat gtggagtgtc actttaccgt
    28801 ttcaacctgt atctgcacca agaccaagtg caagaaggaa tgttgctgat gcaactaatg
    28861 acgtagcaga gaaaaggatt tcaacctatt atgataaaaa gtatctcgat tatttgaaaa
    28921 aaataaaaag ctttatcgaa gatcaaggtt tgttagatga tgaattttac tcgatcgtaa
    28981 atgatccgat gggtgcaatt atggaagtgg acttttacta tcagatacca aaaagctata
    29041 aaaaagtcta taacattatg aaaacgacag cgccagattt agataattta gtaaaaagtg
    29101 ctatggatgg tattttcaac atctcagcta tacgagatag ccatatcaca gggttaattg
    29161 cttttaaata caatgtggca aacgaaggaa aaacagttgt gaggatcaag cgatattcag
    29221 aatttgagtg ggatacttct gaaggattga acggagatat ttgggaagcc acatttgatt
    29281 tcaaaccagt agcaacgcca agaccaaagt ctaaatttag aggaaatggg attatcactt
    29341 actatccaaa agagtataac gactatttgg aaaatatcaa gaatacgctt aaagagaaag
    29401 atttggtaaa tgatcaactg aaaaaggtaa tgaatgctcc tatgggggtt atagctcaga
    29461 tagattattt ttaccaagtg agaaaagata agaaaaagct tgattcactg atgcgtactt
    29521 cacagccgga tattgataac ttagtcaaag gaacattgga cggaattttt aacaaggaaa
    29581 ttggcatcaa agatagcagg gtggtaggtt taatagcttt taaatacaat acttttgaaa
    29641 agtcgaaaac aaacgtgcga ctacaggaga tgggctgatg aataataacg aaaaaattct
    29701 tcatgtactt gatagctttg aaacaattca agaggaacta aagaaatatc gagatgtttt
    29761 ggaacaaaga tatgattttg tcaatcagca aaagagtaat cacatggatt tcattctcaa
    29821 tatgaatgac ttgaagaaga aattagttga acgaaaagaa caagaaaagt tgatcaaagc
    29881 ttactttgaa ctcggtgaaa aagaagttaa aaaggcaatg gaactgaatg aagatcgcag
    29941 ggttttagat cagctgttag aacaattatt ggtgatgttt caaaaaggac gaatagatga
    30001 ggatttaatt gaagaagggt tgagaaagta tcctgcaaac tcaggtatcg gaatagtgct
    30061 gaaagcgatt gatgaagaag aaattgaaga tttcataccg gcagaggatt ttgaatcggc
    30121 aatggaatat ataaaatatt acagccaagg gatcactgct tttagagagt ttgatcctga
    30181 agatgttatt catgacttga acaacctaaa agaatggtgt gaaggttatg aagttgatga
    30241 ttccggtttg gactacttga tcagtattat ggaaatcgaa gaagagatgc cggataagcc
    30301 tgatccaaca gatattttag aattaatcca tgatgctaga aatccaattg catacatttc
    30361 tagaggatat actgtattgg aatactataa gccttatata tcagcgatga accatttaag
    30421 acgtgtatta agggaaaaac gtgaataccg ctcagtgtta aatgcatcga atagattaga
    30481 aaaagctgtt tcagagctag atgcttatta tcgtgaacat tacctgcaag caggtggtat
    30541 gccaagaaat acgaaagcaa atatcagtag gtatataaaa gaagctgagt aggcggagtt
    30601 agcccataaa atggcaaaag gtaacctgaa agaggcgttt tgaatgaaaa gggatgtttt
    30661 tgaattgtac gtattagaac gtctagaaga cggcatgatg gcacaagaga taattgatga
    30721 aattgatgat atgatcagca gatttggtca attggaattg aatgatggaa acaaaaattc
    30781 ggtagtgcta caacaatttt ggttaaataa tttttcgtat aaggatttta tcaaagaaaa
    30841 ggcaaataga ttgtactgac taaattcacg aggatcagtg atgagagctt attgctaaga
    30901 gcagttaggc tcttttttgt ttgtgttaaa ttattttatt ttacaataca catttataca
    30961 caaaaaatgt tgacggatag tacacaaatg tggtaggata tatttgtaag caagttagat
    31021 taacaagtta aaggaaggaa gagcagttat gaaaaaagaa gaactaatag atatgtttca
    31081 aattgttgaa cgagcaaata atatgggaat tatgtttttt gatcgtattt ctttaaagat
    31141 ggatttaagc gttgcccacc aagaatttaa tctacgatta aaagctttat tgatttctga
    31201 tgatgttaat ttcgctcatg atgttgtagg tatacagaac catatagata gagaaaacaa
    31261 acgtatgggt gatggattct taccaagata ttcaagttta tgatttggcg aaagattcaa
    31321 gagtatgtgt cattattgat aggtactctt gaattggaaa ggaggaatga atttaagaca
    31381 acaacgatta tgatcttaaa acaatcgttt atgtaattgg caatcttacg aataattttc
    31441 atgcaataat gacaataaaa aatgaaaagg ggtaaaatca aatgaaaaaa tttttagtag
    31501 gaattctatt cacaatgggg ctagtgctat tatcaccttc agtttcagca gaaagctcta
    31561 tttccatgta tcgtatgtat aatcccaata gcggagagca tctatacact agaagcgttg
    31621 gagaaagaga caatctgaaa cgttatggat ggcattacga aggtgtcgcg tggcaagctc
    31681 caacaagtgg ttccccagtt tatcgcttgt acaacagata taacggtgaa catttttaca
    31741 ccgttgatgc cggagaaaga gataagattt cgcacacagg atggacgtac gaaggtgttg
    31801 cgttctattc ctacaacgga gcaaatagag ttcctgtgac acgcttgtac aacaaatacg
    31861 tgaattggca ccattacacg ttagatcaaa atgaaaagaa cgtaattagt aaacaaggtt
    31921 ggaagattga aggtacgggt tggtatgcaa tgccaaacag cgcaccaaat cctacacctg
    31981 cacctgctcc aagtaatgaa aaagtgttaa atgttcctgt catttggcaa ggtaatacga
    32041 tgttgtgtga aggtgcttcc ctgttagagg gattgcacta caaaggagta gcgacaaatc
    32101 aaaacttaac cagttttgta aactatatgc caagagctaa tgacaataat ccatatcatg
    32161 gctattctgg cgaatggcgc tacaacgtaa atggtacgta tcaaggcatg atgcctgatc
    32221 cagtagttaa atgggcaaaa aatgttggtg gtaatgccgt taatatcact ggttcaggaa
    32281 cgaatgggat caagaatgaa attcgcaatg gtaatccggt tgtaacttgg attacttata
    32341 actatgcttc acctcaattc aaacaaatgc cttggggacg tgcagtctgg aatggacatg
    32401 tggttctcgt ggatggcttc aaaaatggtg cataccatgt tgttgaccct gtatttggcg
    32461 caaaatggat caatgcaagc acgtatgaga gagcatttaa tgtgactgga atggcggttg
    32521 ctgtacgtta attgatagaa ttaataaata aatgaaataa gagcttaatt actgaaatgg
    32581 taaatgggct cttatttttt agaaaaaaac tgttttttgt aatacgaatt tataacaaaa
    32641 aacgttgaat gtagtacaca aaagtggtat attaaaaccg tagagattat gcgaagaggt
    32701 gattttgatg aaccaaagaa gaagaaaaag gctagaagaa ataagcgagc gactatccga
    32761 gttaaaggat gagttagaag taattcaaga agaagaggaa gagtacaaag aaaatatacc
    32821 tgaaaatcta cagggatcag aaagatatga aaaagcagaa gaatccgttg atcaacttga
    32881 agaagctgtt gatggtttat ccgacatatt agaattgatt gaagatatag ctagctagag
    32941 atgattaatg aagatttcag gagtgagcaa aaaatgattg atatttcact gatagagaag
    33001 ctactttttt caaataagta cacagccaaa gagttggcta caagatggca aatatccgaa
    33061 tcagctatta aagcctatcg tcaaaataag ggaagcgtag gatggcgtga ttggaaagga
    33121 atcagcattg gaaaagcaga agacataatg aaacgtgaag gatttcaagc tgacaatgat
    33181 tctaaaaaag ttgaatagtt taatagtcgc caagaaaagt accaggacga ggggaaaggt
    33241 agattacaaa ggaaaacact tttaaataaa cttcgcaaaa gcatcaggtt atgagtggct
    33301 agggctatac gaatgtgatg aaaacaaagg gctttaatgt caaagaaaac gcaatagggg
    33361 gggaacaaaa tttttaaaaa agatatttat gtttggttta atggaaaaaa atatatgact
    33421 actaacaaaa ggtggtcaga gctagtacaa aatacagtta aaaaagtcat gccagatgga
    33481 tatcatttca actatataca atgctcacaa acaactgaaa gtatttattt ttcaatatta
    33541 aatgagaata gactctatta tttcagggta agttcacacc ataggaaaaa gagagaagat
    33601 aatgttacga catttttatc gggaaaatac aatggcttca aagagttaag tgaagccatt
    33661 ggtgagtatc taaaaagtaa tagatttgtc agaatttctg acaaacatat attgatcttg
    33721 ttactttatt tgaataatag cttcaaattg ttttggagag acgacacttt ctactatatt
    33781 gagaacggca tggatattta ttctggtatc aacggcatat taaaacatta tattaaggcg
    33841 ttacttcgat atggggttct ttatcaagaa gatttaagta accaaattaa gattagtaat
    33901 tctggaaagt cattactggc aaaatataaa aaattgcttt ctcctatcta caaaggaata
    33961 gatatggaag ctgtaaattc tgaaacttta tttaatttag ttgttgatga aaagtttgcg
    34021 aatgaatttt ttaaaagtaa aaaaattgaa agactaaata acctgtttcc tgaaaatgca
    34081 ataaaagctt atgtagataa taataaatca cgattataca actggtttat tcccaaacgg
    34141 ttgcgaggaa aaatcaaaat tggcgatcaa gtgaaagtaa ttaatctaaa tcatactaag
    34201 atcgttaccg taacggagtt gtatatagct gaaaaagaag atattacaaa aatgaaacca
    34261 gttattaaaa tactagataa caaagagtga gtggaatata tggtagcaaa aaagaatact
    34321 ctaaaagaag cttctgaaaa atatcaagat atgaaaagac gaggattaat accaaaggac
    34381 aaaaaaggta tatggagaga atttgttaag gaagtcagta gtgatcgaag tggcggtgac
    34441 tttgttggac gatatacagc aaaaggttat agcaggcgct taaggatcac tgttgaatat
    34501 ggaacgaatg aatcagctac agagctgaag caaaggtact tagatacacg aagagaagct
    34561 tcaaaaaaat atgatgattt caagagcaga ggtctaattc ctaagaagaa aaaggggatt
    34621 tggcgtgagt ttttcaaaga agaagatagc aatcggaata agggagactt cattggacga
    34681 tacacagcaa aaggctacac taagaattta gggatcaagg ttgaatacgg aactggtgaa
    34741 agttcatggg aattgagaaa tcgctattta aatacattgt tagagaaata taaagcagaa
    34801 ttattggagt tgttcgatgt taatgatttt atcgagatgg ctgaaaagta tggtcatgaa
    34861 gaaggtttta tgatcaatga cattatggtt attgataatt ttacggattt attagcactt
    34921 gcgaaaaaag caagggattt aagagaaacg cagatgtatc tattcagaaa taaaaacgga
    34981 agaaaccgga gaactgaatc gagtgagatt gaagattact atgatgcctt tacacacgat
    35041 ctgggagcct ttgtagacga gataagtggt gtaatgaaca aatatagggt ctagaataaa
    35101 aaagccggag atgagcagat aaatgggaaa caatagcata tacacacctg aaatgcagaa
    35161 taaaattaaa agacttgtcg gattagggtg gatgacaaga ggaaaattag attcttttag
    35221 tacgatcaaa tatcttttga tggacattta ccgtcagaac aagcaaacag gaatacattt
    35281 tttgggctat gataagccgg aagatgcacc tttctttgtt ccaagttcag cctatagagc
    35341 cagtacctcc ggtagcatgt ataacttgga tttgtttgag gaaggagcaa agatgatcga
    35401 atatgatttc aacgaagcct attcgaggat catgagaaat tatcctttgc cgtcaaacac
    35461 ttatttgaaa gcaaaatata atacagatca agctattttg agagcgataa atagaccaaa
    35521 agtaagaccg tatgaaaatt taaaaacgta tatgttcttt ttggttaatt ttgaaggaac
    35581 agccaaagaa ggaacatatg ctaatccaaa atcacagtcg atgtttgcgg attatggcga
    35641 aaatcccaaa acagctaatg agcctatttg gttatcggag attgagctaa cacttatcct
    35701 tgattattat gatattaaag agatggaagt cctagatagc cacacatttt tgacgaaaaa
    35761 aggattactc aaagaatatt ttgaaagaat cgagccatta aaaactgatc ctgatacgtt
    35821 gaagctttat aaaaatctta gaacgaaagt atttggtgct atcggacaaa aatcattaag
    35881 agaaggagaa acaagcacct tcgataaata cagtatgtat aatcgagcgt attcctccat
    35941 ggtagcaggc gtatttagag atcgtatggt tagatatgaa cagaaatacg ttcatagcgc
    36001 atatgggcta ttaaaaattc gtactgatgg attgtatttc agatccgaag taccagagtt
    36061 tgaagagctg tataaacttg gagtagttaa gaaaaaagtt catacaatat ccaaagaaga
    36121 agtcgaaaaa gctttagaat ttaaaaacaa aggatttgaa aataagggct cctttgggaa
    36181 atctaaagat gaagttaagg gtgttgtgat agacgatgat ttcgagtttg attttgacta
    36241 aatactcttt ggaaaagatg gtaggaggta aatatgaaga agaaaattgt ggaaggaaat
    36301 gtaggtgtta ttattctaaa agattcaact gactatgcta aatcaatggc actaaaagac
    36361 gaaaaagttg cacgcatgga taaagattta gttgaatttc tgatggatga tacagaaaga
    36421 ttaaaagggg atcagttttt gagtttaatg aaagacaaag gcttaaacac atatggttta
    36481 tttgattata agaaattagc tatagagtgg gtaaaagaag gagaaatatt ttttattgaa
    36541 gaatgggaag gtactgagaa cttagttctt catcagtatc ttttgaaagc ttaatctaag
    36601 taaaacagtt ctgactagtt atgcttgtca gaatttcaaa ggaagttttc cataaccatc
    36661 atcattcaaa aataaaggat ttgaaaataa gggttccttt gggaaatcta aagatgaagt
    36721 taagggtgtt gtgatagacg atgatttcaa gtttgatttt gactagattt ttttgcaatg
    36781 tttaagagta aacaaggtaa gaactaagat catgaagatt gagagtaaag aagatagagg
    36841 acgaatgtat gagaacacac tatatttatt tagaagataa aattgtagat gaaattccgg
    36901 caaaaagtcg agaacaagct agaaaattat ttattgaaaa atacaaagag atcaatggag
    36961 atagtgaagt tccaaaagtg gtcgtaacag ttagaaaaag gcaaacaaaa aagagaagta
    37021 cgtttcatgt cagccctgat atatataatc ttatagagcg acatagaagt gaagataata
    37081 tttctatctc tcaatatatt gctacagtta ttgatgaatt gatttcagga aagtatgact
    37141 atgcttttga ttttgagggt gaaaaggatt acaaaagtgt tgctgtcttt caatctgatt
    37201 ttgagaagct aaaaaactat gctggcagga gaaatgttaa ttttgcaaga ctactagaag
    37261 agttctactc cgaagagttg ggagtaggaa atgaatacaa gagagaaact tatgcgaaat
    37321 tgtctcataa gttatcccaa gcgcttaaag aagtgacaga agttaaagcg gaactagatg
    37381 ctaaacttga agaagtgaaa aaatatcaaa cagagaggaa gaacaacatg aagaatcaag
    37441 tggaatttga cgaacagaaa gaatttgaaa aagcaagaaa acgctataac aaaattatga
    37501 atgaacttag ctatgaacac ttaacagttc atgaataatt gagtgagggt acagaaaaat
    37561 atacgatcgc agatatggtt aatgaagtgg aaactctttt agcttctttc tttgaaaatg
    37621 gtcacgtaaa cgatgaatgg cgaaaagaag aacctaaaag ggttaacgct tatattgctc
    37681 gattaaaaag gtttattgat aaatataagg agaatagtga caaattttct aaagataaat
    37741 aaggagagag tgtgattaca tttgccagaa atcgaaggaa tattttcgcc aaaaataggt
    37801 aaaaaacagt tagatttcag cagttttccg agcgtaaata gagattataa tctaggtgat
    37861 gaggtacaga tactaaatat tgatttcaaa gaactagact tcgctgttgc tgtttccaac
    37921 aaacccaagg cattggaacg agtgaaaaaa atataccaag agaatgatca aaatatcaaa
    37981 aatatgatca aaaagtatag acgagataat tcaaaagttg aagacgtttt attcaacggt
    38041 acggcaaaac atgatttgaa tgtttatatg gcattaggag tggctttgta ctgtaattca
    38101 attaatcaga cagatatttt ggttaatatc gtgaggaata gctatcaaaa aatattaaaa
    38161 ataagtagcg aaacaaaccc ttattatgca atgaaatatc ttggggataa tttattaaat
    38221 gataaaaaat tgatgcacaa cactgaagag ctgttatcta atttggctgc gattaacttt
    38281 ctattcaaaa caggttctga agattatttt atagataacg tactaaaagg tgcttacgga
    38341 acagcatttt atccgcttta caatatgaaa cagcataaag ccgaacaaaa ggataacaaa
    38401 cttgaaatta aaaagatgaa agaactatta ggtataggat ttgtaaaacc caattcaaaa
    38461 gtcactattg cttcgttagc aactactaac aatgatggga aaatagtaaa tgggtttgac
    38521 gattcagata tagatatatt gtcaaaatca tatttttctt tgtatggaga ttttgcagat
    38581 ttgatgggta ttgatagtta cttagtcaat aattttgaga tcaagggtag cgagctgaat
    38641 gatattgtgt atttcgtttt aatctctcta tatgcaattg atcagaatac cgagtaccga
    38701 caaattgact atttgctagt tttttatagt ttgttatatc agttcgtttt aactcggttg
    38761 tttaaaaatg ctaaggaaga attgatcctg aattctgatt atgaaatagc aaaacaaatt
    38821 gcaagtatta gaaaagaaaa caaatctgaa gtaatcaaat taagaaaaga attaagtatt
    38881 gcgagtaaag aaaacggtcg gttagaatct ttagtcaaat cggcaaaaaa agaagaggaa
    38941 cttttgaagc aagagattaa agaattaaaa ggaatcagaa aagaacttga acagctgagg
    39001 tttgaaaata aagtattaaa gcaattgcag gagcaagaac atagacaatc attaaatggt
    39061 aaaactgaag agccttcact gtctgaaatg atagcttatc taaaacataa aaacattctt
    39121 ttagtgggag gacatgtaaa ttttcaaaat aaattaactg aactattacc aaatattaaa
    39181 atgtttgatg taagcgaaaa taatggaaag atacttaaaa atttgaatac gtgtgatgcg
    39241 atttttctat atacagatta cttgaatcat ggtctatatt atcgtgttat gagtgaaatt
    39301 tctaataaag ataagatatt ttatttggat aatcatgtga atatgaactt aacaattaaa
    39361 cgtatttacg atcttttatc acttatataa agagagaagt gataaatagg acacaaaatt
    39421 tggaaagtag gtaagtcact atgagagaac taactttatc ttctaaggaa ttagaaaaga
    39481 aaactgcttt attaaacgaa atatttagtg ctgattgtat aatccaaagt ttaaaagaaa
    39541 gttcattttc aaaactaaca gatcaggaaa aaatttctta tatgctaagt ttagataatc
    39601 taggaactaa atatgatgaa tcagcaagta gcgataagct tttgattgaa ttagtatcaa
    39661 gtcttagtag ttttggaaaa atgaaaagat ttaatgtgat attagaaatt tttgcttggt
    39721 taatcccttc atttatttgg tttttttata ttttcatagt tgaatctgag ttaatagcca
    39781 aggtctctct tggactgtta attccgatat aaataggttc agcaatttac tttaataact
    39841 tgtatacacg tattcaaata aaaaagcagt tattacatga aggattaagt ggtaatatgt
    39901 taaaaagaat tgcatgggat aaagataata aaacagatat tgagcttaaa gaatataaat
    39961 acaattattt ctcgagatgt atagatagaa agttactgtt taaaaaagaa ggattagaat
    40021 ttagttttga tgaagatgaa tttgaaaaat tagtgatgga ctggcgtaag aatttactgg
    40081 atacggtaca attctaaaag caattgaggt cgaaattccg atacttgaat catgatctat
    40141 attatcgtgt tatgagtgaa atttctaata aagataagat atttcatttg gacagtcatg
    40201 tgaatatgag cttaacaatt aaacgtattt atgatctttt atatgtttat aaacattaat
    40261 gaggtgctaa aattgacaac aataactatg acaaaaaaaa tagaagtata tgttgatgga
    40321 tcacaagaag aaagggattc cttttttgat tttttttgga aaaatagtag agaatatgta
    40381 aaagcgttga atctggtata taaacagcta atgtttgaag agttgataaa agatgcaatt
    40441 aaagaaatgg atcaaggtta tgctgagaga gaagaaagta ttcaaaatcg gattgaaaag
    40501 ttagcatata ctgatccaat agatcaaaaa aaacttgata aagcaaaagc tgaattatct
    40561 aaattgagaa atgaaaaaaa taaagaagcg agagaaatat taacgcaagc gatagggttg
    40621 aagagtcaaa cgagaacacg agatgtagca aaaaaattag actttgactt tggagatttt
    40681 gttgattccg caaatatgaa agctagtaaa gattttggca atgattttat cggaattgtg
    40741 accggacaaa gaacgcctag aatatataaa aatagaaaca aatatggatt actacctttt
    40801 agaggtcgag atattaaaat ccataaagat gataagtttt atatttattt acctaaaggc
    40861 tttaggttta aagtgaattt aggtagcaag ccaagaaaag caagagatgt tgcttcagtt
    40921 ttacaacaca ttatggatgg cgattacaaa ttaaatcaaa gtaatcttat ttttgatgga
    40981 agaaaattat tttttaatct aacaatttct tttgaaaaaa gctataatga aatggaattc
    41041 aagaaaggta atgaattgat cgtaaacttt gcagatttgt gtagcggtga atgtttaatt
    41101 aatgataaat ctgtaatgtc ttttggtgat agccaatata taagaaactt caaaagtaaa
    41161 attttagcaa tgaaatcaaa agaacaaagc cgaggagtct tttctaaggg cggtcatggt
    41221 cgaaaaagaa aattagatca agatcgatgg gaagcaataa agaaaagaga aagtaatttt
    41281 gttaaaactt ataataatca atgttctgca aaaatcgtta aaactgctat tcgcaataaa
    41341 gctcaaatta ttcgtatcat tgaaccagat aaggcttatg gcgaatgggc ttactatcaa
    41401 ttgaaagaac aactagtaaa caaaggaaaa agatatggga tagaagtcag aagttagtaa
    41461 attaaacaat cgttgatttg gcaacgaaat agtgagggaa aatccactca ctgaagggta
    41521 aactccgtca agttgtgtga ccagacggaa ggacgaatgt tgttgaggtt ttagaaccat
    41581 ataaaacagc atgtttctca aatttttagg ataagggaaa ttgctagcag tttgttttag
    41641 aaccatataa aacagcatgt ttctcaaatg agtactttaa attaattgat ggcgttcctg
    41701 ctttagaacc atataaaaca acatggattt aaaacaagaa gtaagtcagt tctcctttta
    41761 ctttagtaag gaaaaataaa gtattctcaa taaaacttaa aataagcaaa cctttgtttt
    41821 aatagctaat cgaagtaaaa cccaaagcca tatgaacccc tgaatcagcg aaagtttatg
    41881 atgatttgga ttactaatag tttaggaatt agatggtaaa tctaaattaa acaatcgttg
    41941 atttggcaac gaaatagtga gggaaaatcc actcactgaa gggtaaactc cgtcaagtta
    42001 tgtgaccaga cggaaggacg aatgttgttg aggttttaga accatataaa acagcatagt
    42061 tctcaaattg tttaaaatga ttgtcaagtt aaaaaaatct gggatcatat aaaatagtat
    42121 agttctcaaa tatgattaga aaccctatta aagagcaata ttaagccaac ttacttaaat
    42181 cgtttaatta agaacaataa tctcttttag tcagactttt agtctgactt ttttgtattt
    42241 ccaaaattta ttctttacaa aaacgaacat acgtttgtat aattgcttgt gaatggggga
    42301 ataaaaatgt ttgattacag tagagaaccg gtgaatgact attttttaat cgacatgaag
    42361 tctttctatg ctagcgtgga atgtattgaa cgaaatttag acccaatgac tacaccgtta
    42421 attgtcatga gtagatcgga caacactggt tcaggtctga ctctagcttc ttcacctact
    42481 gcaaagaaaa aatatcatat tactaatgtt agtagagcta ctgatttacc ttatccatta
    42541 ccaaaagaac ttcatattgt tccaccacga atgaacttat atataaaaaa aaatatggag
    42601 gttaatcaga tttttagaaa gtatgtcgca gatgaagatt tattgattta ttcgattgat
    42661 gaatcaattt taaaagtgac aaaatcctta aatttattta ctagttcagg aacaagaagc
    42721 gagagaagaa gagaattagc taaaataatc aaagatgaga ttcagaaaga aacaggtcta
    42781 atagcaacag tcggaatagg agataaccca ttattagcta aacttgcact ggacaatgaa
    42841 gcgaagagta gtaaagaatt tatagctgag tggacttatg agactgtacc tgaaaaaata
    42901 tggaaaataa acgagatgac tgacttttgg ggcattggat cacgcatgaa aaaaagattg
    42961 aatcatatgg gaattatgag tatagaagat ttagcaaatt ggaatcccta cacaattaaa
    43021 agtcagttag gtgtaatcgg actccagtta tatttccata ccaatggaat tgatcgtacg
    43081 gatattgcta ttccaccaga accaaccaaa gaaaagtctt atgggaattc acaagttcta
    43141 ccaaaagatt atacgaggag agaagaagta gaactagtag tcaaggagat ggcagagcaa
    43201 gtagccattc gaattagaca acacaattgc aaaactggtt gtgtacgctt agcaattggt
    43261 acgtcaattt tggaagaaaa atcaggtttc tcgcaccaga tgaaaatacc aattacagat
    43321 aatacgaaag aattacaaaa ttattgtctt tttttattca ataagtttta tgaggggcag
    43381 gaagtacgac atataggagt aacttatagc agattagtat acacagattc cctacaatta
    43441 gatttattta gtgatccaag aaaaaagatt gctgaagaaa atttggatag gattattgat
    43501 aagattagac aaaaatatgg atttacagct attatacatg cttcatctat gttagaaggt
    43561 gcaagaagta ttacgagaag cactttaata ggaggacacg caggaggatt aggaggtttg
    43621 cataatgatt gaatataacg atagaggacg tttaaaatgg tcgggatttt atttgtctga
    43681 tcatactgaa agaatcaatg cagacaatgt acaacgtgct aatcataatt tggcaaaaga
    43741 acaaatgacg actgaagaga ttgcgaaaat actgagaact gctgtcataa aaaatagacc
    43801 cgtctgcatt caaaaagagg aacgaaacgc tgaaggttac tatccagatg atgtagtagg
    43861 aaaaattatt ggatatgacg agcttggtgt atttgtggaa gaagaaaaag ttcactatga
    43921 tgaaattagg aatgtttctt tctcggaatt attaagatgg agtcagatat gaacattatt
    43981 aaaaaatacg aattcggtta tattacgtat gaacagctaa tagaggaatt atggggatat
    44041 ggtcaacaat taattaatga agtggggatt gattgctttt gtttctatat tgaatccgga
    44101 gcaggatacc ataagaacag atactatata tttccatatc caccaaaata gtctcttaga
    44161 tatttttaaa ttttctccaa atactttcaa ttattgtgaa atatttccct ttaattaatc
    44221 aaatttttta taaaagtaat atataaaata aattgcttga agtattatat ttagatatca
    44281 taatttatgg aagaaaaatt aattaaaaag gatgagatta taatggggtt actggttccg
    44341 agagctaata cttatactat acctttaacg aaacgccaat taattgctaa aagatatcaa
    44401 aggattacta gagccatcaa tagagagttt tggaattctg aaagtgatac tgctcacagt
    44461 ttatacgtag gttcttatgg gagaggaacg gcaattagta ctagtgatat agatataata
    44521 gttgaattac cgatggcaga atttgacaga ttcaaaaatt atttatcaaa tggcccgtct
    44581 aagttattac aagttataaa aaacgcattt caggaaatac taccaaatag tgatattaga
    44641 gctgatggac aagttgttaa aataaatttc catgatggaa tcaaatttga aatagttcca
    44701 gcttttaatg aaaaagatta ttggggtgag tcaaaagggt ttatttatcc agattcaaac
    44761 atgggaggga actggaaagc tacaaatccc aaaaaagagc aagaagccat gaaattaaag
    44821 aatacaaaaa gtaataattt attatatgct acgtgtaaac attttcgaca tgtacgagat
    44881 acagaattta cgagttatca tctttcagga atagtaattg acagtttcgt atatgaggct
    44941 atgggaaatt ggaaattcgt tgaaaataac agtgggggtc aaaacattag ttctgtatct
    45001 tatgagacag ctttattaga atactataac tcacataaag ttatgggcgg tttaaattta
    45061 tactctcctg gaagcaatca atttgttaat tctgatagta gtatcatatg tttagaaaaa
    45121 gtattaaaaa aaatagcttt ataaagtttt atggaggaac aacatgggag acgaaaaaaa
    45181 gcgcttggaa gttcaattac gtgaagcata tggacgagta acgtatactt acaccactca
    45241 tttaaagcaa gccgataata ttatttctga caataaaatt ttaaggtgtc ttcaaattgg
    45301 tttgtcagcc cttactactg gaggggtact tagtaccatt attactgata atagtgagtt
    45361 aacctttgtt gctggattat gttcaacaat ttctcttgga ttaaacttat actttaagaa
    45421 ttttgatctt gctgaagaag ctaaaaatca tcaaattgtt gccgataaat tatggttgat
    45481 cagagaagaa tacgtaagct tactaacaga tttaaatcgt ttaagtgttg aagatattgt
    45541 gaaaaaaaga gatcaattaa tgaacgaaac tcatgaagca tataaaaatg cccctaaaac
    45601 aaatagtaaa agctatgaat cagctcagaa agcattaaaa tctgaagaag agcaattttt
    45661 tacagaagag gaattggata agatgttacc agagcatctt agaaaaaaga gttctaaata
    45721 ttgaactctt tttttatgct taaaattctc ttgtaatttt tctattcatc tatatttaag
    45781 aagaaatatg atgatagaaa catctcctaa agccaccgca agccgttcta agcatagctt
    45841 agcccgacat tgctagatat gctcatctca ccatgaacgt tt
//
LOCUS       DACCKU010000013        44604 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.013, whole genome shotgun sequence.
ACCESSION   DACCKU010000013 DACCKU010000000
VERSION     DACCKU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 44604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44604
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.013"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(5..586)
                     /locus_tag="IVN13_000663"
     CDS             complement(5..586)
                     /locus_tag="IVN13_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7011179.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IVN13_000664"
     CDS             complement(769..1395)
                     /locus_tag="IVN13_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7011180.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IVN13_000665"
     CDS             complement(1417..2748)
                     /locus_tag="IVN13_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP7011181.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IVN13_000666"
     CDS             complement(2903..3304)
                     /locus_tag="IVN13_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7011182.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IVN13_000667"
     CDS             complement(3390..3596)
                     /locus_tag="IVN13_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011183.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IVN13_000668"
     CDS             complement(3691..5193)
                     /locus_tag="IVN13_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP7011184.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IVN13_000669"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IVN13_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP7011185.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IVN13_000670"
     CDS             complement(5764..6381)
                     /locus_tag="IVN13_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011186.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IVN13_000671"
     CDS             6602..7975
                     /locus_tag="IVN13_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7011187.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IVN13_000672"
     CDS             8050..9162
                     /locus_tag="IVN13_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP7011188.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IVN13_000673"
     CDS             complement(9234..10871)
                     /locus_tag="IVN13_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP7011189.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IVN13_000674"
     CDS             complement(11039..11647)
                     /locus_tag="IVN13_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7011190.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IVN13_000675"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IVN13_000675"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP7011191.1"
                     /translation="MTLKEEIIQESKRLGINKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IVN13_000676"
     CDS             complement(13201..13953)
                     /locus_tag="IVN13_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011192.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IVN13_000677"
     CDS             complement(14094..15044)
                     /locus_tag="IVN13_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7011193.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IVN13_000678"
     CDS             complement(15045..15884)
                     /locus_tag="IVN13_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7011194.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IVN13_000679"
     CDS             complement(15881..16627)
                     /locus_tag="IVN13_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7011195.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IVN13_000680"
     CDS             16865..18235
                     /locus_tag="IVN13_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7011196.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IVN13_000681"
     CDS             complement(18290..19186)
                     /locus_tag="IVN13_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7011197.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IVN13_000682"
     CDS             complement(19467..20144)
                     /locus_tag="IVN13_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7011198.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IVN13_000683"
     CDS             complement(20235..21140)
                     /locus_tag="IVN13_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7011199.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IVN13_000684"
     CDS             21315..22769
                     /locus_tag="IVN13_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7011200.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            22785..23816
                     /locus_tag="IVN13_000685"
     CDS             22785..23816
                     /locus_tag="IVN13_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7011201.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IVN13_000686"
     CDS             23823..25010
                     /locus_tag="IVN13_000686"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP7011202.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IVN13_000687"
     CDS             25166..26548
                     /locus_tag="IVN13_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7011203.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGRGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26675..26905
                     /locus_tag="IVN13_000688"
     CDS             26675..26905
                     /locus_tag="IVN13_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011204.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27105..27773)
                     /locus_tag="IVN13_000689"
     CDS             complement(27105..27773)
                     /locus_tag="IVN13_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP7011205.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27962..28759)
                     /locus_tag="IVN13_000690"
                     /pseudo
     CDS             complement(27962..28759)
                     /locus_tag="IVN13_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(29040..30419)
                     /locus_tag="IVN13_000691"
     CDS             complement(29040..30419)
                     /locus_tag="IVN13_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7011206.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(30594..31763)
                     /locus_tag="IVN13_000692"
     CDS             complement(30594..31763)
                     /locus_tag="IVN13_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7011207.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGVVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(31738..32889)
                     /locus_tag="IVN13_000693"
     CDS             complement(31738..32889)
                     /locus_tag="IVN13_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP7011208.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(32849..34210)
                     /locus_tag="IVN13_000694"
     CDS             complement(32849..34210)
                     /locus_tag="IVN13_000694"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP7011209.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(34207..35208)
                     /locus_tag="IVN13_000695"
     CDS             complement(34207..35208)
                     /locus_tag="IVN13_000695"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP7011210.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(35224..35919)
                     /gene="dapD"
                     /locus_tag="IVN13_000696"
     CDS             complement(35224..35919)
                     /gene="dapD"
                     /locus_tag="IVN13_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP7011211.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(35925..36641)
                     /locus_tag="IVN13_000697"
     CDS             complement(35925..36641)
                     /locus_tag="IVN13_000697"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP7011212.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(36638..37519)
                     /locus_tag="IVN13_000698"
     CDS             complement(36638..37519)
                     /locus_tag="IVN13_000698"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP7011213.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(37522..38322)
                     /gene="dapF"
                     /locus_tag="IVN13_000699"
     CDS             complement(37522..38322)
                     /gene="dapF"
                     /locus_tag="IVN13_000699"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP7011214.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(38309..39568)
                     /gene="lysA"
                     /locus_tag="IVN13_000700"
     CDS             complement(38309..39568)
                     /gene="lysA"
                     /locus_tag="IVN13_000700"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP7011215.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(40054..40617)
                     /gene="efp"
                     /locus_tag="IVN13_000701"
     CDS             complement(40054..40617)
                     /gene="efp"
                     /locus_tag="IVN13_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP7011216.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            41045..41962
                     /locus_tag="IVN13_000702"
     CDS             41045..41962
                     /locus_tag="IVN13_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP7011217.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            41975..43120
                     /locus_tag="IVN13_000703"
     CDS             41975..43120
                     /locus_tag="IVN13_000703"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP7011218.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            43291..43749
                     /locus_tag="IVN13_000704"
     CDS             43291..43749
                     /locus_tag="IVN13_000704"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP7011219.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            43908..44378
                     /locus_tag="IVN13_000705"
     CDS             43908..44378
                     /locus_tag="IVN13_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP7011220.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(44431..44546)
                     /gene="rrf"
                     /locus_tag="IVN13_000706"
     rRNA            complement(44431..44546)
                     /gene="rrf"
                     /locus_tag="IVN13_000706"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        12494 a         9257 c         8080 g        14773 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgttaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagatcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagaggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga
    26641 agaagcacgc aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat
    26701 actgacagaa gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga
    26761 atttggaaga ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta
    26821 ctttatgatt gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga
    26881 aacaaaaaaa tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata
    26941 agaaaggatt tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga
    27001 agaagcaact tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt
    27061 tttgagtaac aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg
    27121 taagattcat gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat
    27181 tcaacataaa tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc
    27241 catgcttcag tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca
    27301 agcaggatcg ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca
    27361 ttctctagga taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt
    27421 gctcgcaact gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg
    27481 tctttgatta gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg
    27541 acgatacgtt cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc
    27601 cctacatgga cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta
    27661 tagactaaat caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact
    27721 aataataaac cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct
    27781 ccccctaact atctgttaat taaattatat atgctttctc acgaacacgc atggaatata
    27841 caaggaagaa atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa
    27901 agttagactt ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt
    27961 tttacacaac cgttacggtt gttttacgaa attggacgag actttgccag tttaatttct
    28021 gttttatccg ttcattattg tagtaataga tatagctatc gatggctgat tttaattcct
    28081 cgaaacagtt ataaactttc ccgtgaaaaa tctcttgttt taataagcca aaaaaattct
    28141 ccataagtga attatctaag cagttccctt tccgagacat gctttgaaag attttgtact
    28201 ccttcaattt gtttctataa gctttcattt gatacgccca accttgatct gagtgaaacg
    28261 tacgacgata gggacaatcg ttagtggtct ggatagcttc ctccaaacct tccattaccg
    28321 ctaatgcatt gggcttttca gagatacgat aggataaaat ctcactattg aacatatcca
    28381 taaaaggatc tagatacaat ttcttttgac ggataatgcc tttggcatcc acttcatggt
    28441 atttaaattc agttgtatcc gtcgtgattt tctgatggca gatactggtg tagaaacgac
    28501 gatggatccg atttttggct actgctccaa cttttcctcg ataagaattg tagcgacgtg
    28561 atttacgcgt gtatgccgtt acttttattc ctagcttctt gatcaagcgc tgaactttct
    28621 ttttattgat agagaaaccg cggtttctta gttcattggt catccgtaaa cagccataat
    28681 ccttgtgctt ctcccgaatt ttaagcattt ccgcttcaat ctcttgatct ggatttttac
    28741 ggtccaatcg cctttgccat ccaaataatc atgaacgatc tttaatttaa gttcaaaagt
    28801 atatttaacc ataaaaaata ccgacctcca aaagttagat tttttggtct aacttttggg
    28861 ggtcggtaca caaatcgtgt cacagtccct tttttgaaaa taaacggtgt tcaaaagctt
    28921 gcatctgcgg taataagtcc agccaagtta aaaacataag aagctgtttt tacttggctg
    28981 ttcttattac ttgatgcaag gcagcttttt cacaacctct tttcaaatat ttcgttttct
    29041 tattctacaa atggttcttc tcctaacgct tgttgagcgg ctagattcaa catgctccat
    29101 tgtcggtcaa agcctggctg gaagaagaag tcagcttctg ctagttcttc taaagtcagt
    29161 ttgttttgga tcgctagtgc caatacattt ccttgacctg taacatcata ggtagataat
    29221 actgctcctc ctagcaattg atgggtcatt ggatccgaga ataattggat ggacactttt
    29281 ggatttcctt gttcttctgg tacatatttt ggtcgtaacg tatcttcata ataagacact
    29341 cgtacatcta ctcctgcttt tgctgctgta aagctattca atccgcttgc tgcaaaatga
    29401 tagtcaaaga cacttaatgc agaggaacct acgactccgc cgaacggttt gtttggtact
    29461 tcttcaaaaa gatgttttac tacataacgt gcttctctac gtgcaacagt cgctaaagcg
    29521 atcggcattt ttttgcgtgc tgggatagaa taagcaagtg tagcatctcc tactgcatac
    29581 acatctggaa gattcgtttg caagtattca tttgtttgga tccagccacg ttcatctaag
    29641 tcgacgatcc cttttaacca ttctgtattt gccttcacac cggcagcttg aatgactaga
    29701 tctgctggga tttctttttc acttgttttg attgcctcaa cttttccgtt accagtaaaa
    29761 gcttcgattt ttgcatttgt caaaacttca ataccgtttt catttaggtg ttgttctaaa
    29821 atatctgtca tctcagcatc taaataagtg cctaaaggtc tatcgatcac atcaagcaat
    29881 acgacttctt tcccagcttt tcggcttgct tctgctgctt cgatcccaat gtaacccgca
    29941 cctacgacag cgattttttt aatagccgga tcatttaatt tttctttgat tttggttgcc
    30001 caattgtatc cacgcatcaa gtagacattt tctagatctg ctcctggtac tggcagattg
    30061 tttggtttga caccagaact caaaatcaat ttgtcataag gatattcttt tgtttcgtta
    30121 tctgatgcgc tagtaaccgt tactgttttt ttatcggcat cgatcgtttt tacttcgtga
    30181 ttatttaaaa tatgaacatt ggattgcgga aagttttctg gtctgaaatt acgtacatca
    30241 tttacatctg ttaccttgtc ttctaaatac agctccatac cacatgacat gaaggaaata
    30301 tagtctcctg cttcaaatag cgtgatatct acattttcac cgtatcgtgt caatagttcc
    30361 aagatcgatt gatgccctcc atgagatgca cctacaatga caactttgat tttttccatt
    30421 gatgaaacct ccttgaatat tcttttaaaa tcattctaaa caatttaaac ttaacattac
    30481 attagttatg aatcaaaaga aacgcgttca tttgaactaa atttaaaaaa caatcgtctc
    30541 cttgcagaat ataccgcaaa attaacgatt gtttttttga taaaatctat gaatcactgt
    30601 tctgttaaaa agtctttgat ccgtttgcag gcctcatgta atttttccat gctgctagca
    30661 taagaaatcc ggatataacc ttctccttct gggccaaatg caattcctgg gatacacgca
    30721 acttttgcct gttccgctaa ttctacacaa aactgccaag agtcagtttg aatggcagct
    30781 gggagtttgc aaaacaagta aaatgcaccg tttggctggg tcacttcaaa tcctaaaggc
    30841 gccaattgct ggaccagata gtctcttcgt gtttgatatt gctctttcat attctcgcta
    30901 tctttcaccc catttattag cgcttcagcc gcagcttttt gcgacatcgt tgtagcggca
    30961 gtcaccagat attgatggac tttaattagt tcgtcaataa gggctttttg agcaaaaata
    31021 aagcccactc gccaaccagt catcgcatgt gacttggata acccgcttat cacgattgtt
    31081 tgttctggaa gataagtagc tatggacata tgcgttccac tataagtcaa ttcagcatat
    31141 acttcatcac ttataacaaa taccggatat ttttttaaaa cctcagcgat tgctttcaat
    31201 tgttttgcag acaatgtcgt cccagtagga ttgttaggat agtttaaaat gactgctttg
    31261 acagctgatc catattgttc aaatgctcgt tcaaattgtt ccggctgaca gacaacccct
    31321 gtgtcagaag tatctatcgg gatcaattca gcaccagcta gttccactaa tggctgatat
    31381 cctgaataag caggcgctgg aataagaacc ttgtctcctt cttccaaaac agataacaac
    31441 gcagaggcaa tcgcttccgt tgcaccaact gtcgtcaaaa cttccgtttg cggatcataa
    31501 gttaatccat aacgctgttg ctgaaatata caagccgctt cccgcagttc ctctaatcct
    31561 ctcattccgg tatagtgaga aaagtcttct tcaatcgctg ctattcctgc ttgcttgaca
    31621 tgttcaggag ttggaaaatc cggttctccc agggtgagtt tgatcacatc tggtatcgac
    31681 gatatttgct gatcgaattg tcgtattttt gatacttcta ttctagaaag cctaggacta
    31741 aatcgattgg ataaattcat cattatttac tcgctcctct tttgaactgc tatcgatttt
    31801 tacaagaaga ttttctgtaa aaagaatcgt tttgtaccat tctacaccat tttctaaaac
    31861 atccatatca aaatcgaacg tcggatcatg taaagcaggg gtatcaccga cacctaaaaa
    31921 gaaaaataca catggatatt gttgcgtata tatgccaaaa tcttcggctt gaagaactgg
    31981 agcatctaat tcatgtagtt tgtttgaatc taacagtgct gcatataagc ctctatggtt
    32041 gattacagct ggatagccgt cattatagcg aaaagaaata gtagctgtag agtgaaactg
    32101 ttctgctaaa tggtctaatc ccctcttcaa ataattttgc atatctgaac tgtaagaacg
    32161 gatacttccg gatacaataa cttctccagc taaaacattt cggatcgttc caccttggat
    32221 ttggccaaat ttcattagat gattccactc tgccggtaat tgccggtcaa aatcataaac
    32281 attcttcaaa aatcgtacag ctgcctccag actatcgatt cctttttcac tagaagcaat
    32341 atgtgcagac tggcctttaa tgataatgtc tgtttcactg ctttgtgcca tcaaaggtcc
    32401 tggtcgagag aaaacttctc ccttagctag tccaggccat agatgaaagc caaatattgc
    32461 ttgtacttca tatttatcca acaatcctga ctgaataacg ctttgtgcgc caccggaaat
    32521 ttcttctgat ggctgataga tcagtactac attataaata agatcctcaa tgtgtttagt
    32581 tataaagtca gtcaacccta aaagcatcgc catatgtccg tcatgaccac atgcatgcat
    32641 ataaccttca tgcttagaag caaaagagag attcgtttct tctttgactg gcagtgcatc
    32701 gatatctgtt cgaaaagcaa tcgtttcttc ttgctgatta tcaaaataaa ggagtagtcc
    32761 tgtttcgccg atttcatgga taacggcctc gctgtttttt acttgttcat ataaatattt
    32821 ttttgtcttg aattcatgaa acccgatttc aggaatttga tggagtgcca ttcgatattc
    32881 tttcagcatc cgtattcacc atcccttcat agataccctt cactactgtt tcatattgat
    32941 tattcggtac accgatgatc agattcaact cctgtgctcc ttgtgataag atcgatgtgc
    33001 gaatttctaa tttgttcaat atcgataaca ctttcccaga taatccaatc aattctttat
    33061 gcggaccccc aacgacagag atcaaagcta agtcttcgat cacgacgatt tcttccacgc
    33121 caagtgtttc ttttaattct ttcgtgatga gaaataactt atcggcaatc gcttctgctg
    33181 agacgatcac tccgatattg tcgatccctg aaggaatatg ttcgattgat atctcatgtt
    33241 tagcaaaaat gctcattgct ttccagataa acccgatttc atcggacata tgacgtttga
    33301 ataaagtgat ggaaagaaaa tccttgcgtc cagctattcc tgtcagtcca ttttcctttt
    33361 ctatttcgtg attcgatatt tttgtccctt gagcgaaagg atcattggta ttttttattt
    33421 ggatggggat atcagcctct tgaactggga aaacagctgc ttcatgaaga acgttagccc
    33481 ccatataagc catctctctc agttctctaa aagagatttc ctcgatgatt ttaggatgat
    33541 cgatgattcg cggatcagcc atcatgattc ctgaaacatc cgtccaattc tcataacttt
    33601 ccgcttgcaa catcttcgcc agtatcgcgc cggtaatgtc gcttccccca cggcccaata
    33661 atttttcttt tcctgcaggg ttcgccccgt aaaaccctgg aacaaccatt ttcggatatc
    33721 ttttaaatgc ttttgtgatt tcctgtgctg ttttttcctg atcgattttc ccatcatagt
    33781 caaagaaaat caggtctttt gcgtcaataa actggtaacc aagatactca gccatcagtt
    33841 gagcagtcag atattcacct ctacttacta aataatcttc agttatcttt tcgtcttcca
    33901 gttgtttctg tatgcgtgct agttcatctt caataggtaa ctgcagggca agaacatctc
    33961 ttacttgaat gaaacgatca caaatccttt tccaaagggg tgtccaatca acatgatgct
    34021 gaaggtaagc ataaatcaaa taaagtaagt ctgttacttt gtcatcatct gacgtacgtt
    34081 tgcctaaagc agaaacaacg atcacttggc gattgggatc tgaaagaaca attgtacgga
    34141 cttttttgaa ttgctcttca tgagacatcg atgttccgcc gaatttcgct actttcagcg
    34201 tattactcat cttgccacct ccgctatttt ctgcattaaa gctgcagcaa tctgtacggc
    34261 attcgcagcg gctcctttgc gtatgttatc ggcagtcgta tagaacaaca atccgttctc
    34321 taaagaagtg tcttttctga tcctcccaac ataaaccata tctgttcctc tagcaagaat
    34381 gctcgtcgga tatttatgtg ctgctagatc atcttctaat tgaatccctg gaaactggct
    34441 gagacactct ctaatttctt ccgtcgtaaa ctctttttgc agttgaactg ccacagagac
    34501 tccatgtcca ttttcgattg gcacacgtac gcaggttgct gatactggca aagcaggctg
    34561 atgcagaatc ttacgggtct catttatcat tttctgttct tcttttgtat acccattttc
    34621 caaaggctga tcgatttccg gaatcaccgt ttggctaata ttataaggat acaaaactgg
    34681 ttcctcgtca ttttgcgtac gatgaagatc ttcaatgcct ttttgcccgg aaccagaaac
    34741 tgcttgatag gtcgaataat tgactcgctg gataccaaag gcttcttgta aagctttaag
    34801 cggaagtacc gcttgaatgg ttgaacaatt tggatttgca atcagccgat tcaatgtata
    34861 gtcttccaga ttgacctctg gaacgattaa tggaatatcc gaatcttccc gccatgctga
    34921 ggaattatct atgacaaata gtccctgact tcgtgcgatt ggcgcaaact ctttggatac
    34981 agaagcacct gcagaaaata gcgctaaatc atatccttca aatgaagttt ctgtcaattc
    35041 ctgtacagtg tgtttctttt tcttgaatac acaaacttta ccggctgaac gttttgaagc
    35101 taataatttc aatcgtccta ctggaaaatc atattcttct aacactttga gcatcgtttg
    35161 gccgactaat ccggttgccc ccacaataca ggtatctatt tcgcgcattt ttctccatct
    35221 cctctataat tcgcgtaacg cagcaatcaa tgctgtattt tcttttgtct gatcatcact
    35281 tatttttaat acacgtgcag gtattccacc gacaacagtt tctgctgcca catcttctaa
    35341 aaccactgct cctgctgcca cgactgcatc ttttccgata tggacacctt caacaatcac
    35401 tgcattcgca cctaccagta cgccgtcttc tacgataacc ggtttggccg atgctggttc
    35461 gatcacacct gctaagacag ctcctgcacc aatatggcag tttttgccca ctgtcgctcg
    35521 tccacctaag acagcaccca tatcaatcat cgtgttttca ccgattactg caccaatatt
    35581 gataatcgct cccatcatga tcacggcatt gttgccgatt gacacttgat ctctgataat
    35641 agcacctggt tcgatacgag cattaatttt tttaatatct agcaaaggaa ctgcgctgta
    35701 tcttgcagca gtttcgattt cgatttcaat gattttttct gattcagctt ctaaaaccgg
    35761 agcaatcgtc tcatagtcac caaaaacaac gaagctgctt ccttcaccaa aaactttact
    35821 ttctttaaat tcgattggct cggttacatt cagatagact ttcacaggcg tctttttaga
    35881 tgccgtttga atgtagcgga tgatttcttg agcttccacg ttttttacac ccctttgtcc
    35941 attaataaat cttctaattt gtaataacct ggtgactgat ctttcaacca gtccgctgct
    36001 tctactgcgc ctgcagcaaa gattcgatta gaaaaagatt catgtttgat cgtaaatatt
    36061 tcatcagtag cagcaaacaa tacatcatgt tcaccaggta aagaacctgc acgaatactg
    36121 tgtacaccaa tttggttttc ttctcttttt ttcccttgcc agttgtatac gggctgcagc
    36181 ttttgcactt cttggattgt ttgaagcagc atatttgcag taccagatgg tgcatctttt
    36241 ttacgactat gatgtttttc aatcagttcg atctgccagc cttgcaacat tgcagctgct
    36301 tgcttgatta gttgattcat caccatgatg ccgaacgaaa aattagcact atacatcaca
    36361 ggtacatgtt tggcatgttc ttttatttct tggaactgtg attgtgtata acctgtcgtg
    36421 ccaatgacta acggaagttg atatgataag ctatacgaaa ggattttctc taaattatct
    36481 ggatgagaaa aatcaataag cagttctggc agttccgtaa aagcaagcgt agttgcttct
    36541 ttaggtgccg tccgtaaaag aaggaccgta tctcctcttt cttgtgcaac ttcttcgact
    36601 ctatttccca tctgtccact gccaatcagt ccgattttca tttttcaacc gtcctttctt
    36661 ttaacaaaag ctgagctaat tgtttctttt tctcttttga aagagaaact aaaggcaggc
    36721 gtagaggtcc ggaaggtaag tctaataatg acatggctgc tttgacagga ataggattgg
    36781 tctcactgaa caaggcatgg atcagttcaa gtgcatttaa ttgcttttct tttgcttgct
    36841 gccatcttcc gtcctgcgta tcttttacta attcatggac tattttaggt tggatattcg
    36901 cccatacact tattacaccg cttcctccta cactcattac tggtaggatc aaatcatcat
    36961 ttccactgta aaggaagaat tcttctccaa tcaaccgagc ggcatccatc acataagcca
    37021 tatctcctga ggcttctttc aaaccaatga tattaggatg ttctgctaaa tttactaata
    37081 catggattgg aatcgacatg cctgttctag aaggaacatt atacaaaata agcggaatcg
    37141 gtgaggcttc tgcaattgct gtaaaatgag cagctagtcc tgcatcactt gttttgttgt
    37201 agtaaggggt gatgaccaaa agtgcgtctg cccccaaaga agcaaaatgc ttcgcttttt
    37261 caatcgtttc tttcgtatta ttcgttccag ctcctgcaat caccggcaca cggccagcca
    37321 ctttctttac tgtcagttga aggatctgct cctcctcaga ttgtgtgagc gtagaagatt
    37381 ctcctgttgt acctaaaact aataatccat ctgtttgttc atctaaatgg aattggatca
    37441 atttttccaa accagcataa tctactgatc cctcttcatt catcggcgtg atcagcgcca
    37501 cgatcgaacc ttttatcatc ttcattcctc caatattctt gtccattcat gaactgctgg
    37561 gccagccatt tgtatcgcat cctcaccaga gatcatcaag tctccttgtt ccaagtgaac
    37621 aactacctga tctgctgaaa ttttcccagt ttccttagca accacataag aggcacagca
    37681 acctgtaccg caagcaagtg tccaacctac tcctctttca taggtacgga cgatcaattc
    37741 ttgttcattg actacttcta caaagttgac attggttttt tctttgaaca aaggatgatg
    37801 acagatcagt tcacctcttt ttctccagac ttcctcacgc gcattttcaa caaaaacaac
    37861 ggtatgaatc gttcctaaaa ataaactagt gatcgttact ttttcaccat tgatctctat
    37921 ttcctggttt tttattggat aagtttgatt agctttgatt ttttccggag aatcatctgg
    37981 cactcccata tctacgctgc aataaaaagg tgagggatcc aacaccgtaa tttttcttgt
    38041 gcctgctaat gtctctacgt tgaattcctg ttcattcatc atgttctgat catttacgta
    38101 tcgagcaaaa cagcgaatgc catttccgca catcggcgcg cggctgccat ctttgttgta
    38161 atagatcatt tccaacggat cgatttttac ggcgatcaat ccgtctatat catatttttc
    38221 ttttgtgcat aaagaagcgg ctaattgact ataatctgtg tcttctgcgt aatctaataa
    38281 gataaaatca tttccgcagc cattatattt atgaaggatc ataggaaact tcccccctta
    38341 gtagatcaga aaatgcttgt ctgcgaacaa tcagtcttga ttggccatta ttgacaaaaa
    38401 caactgctgg tcgtgtcgct cgattgtagt tgctgctcat cgagtatcca taagctcccg
    38461 tggaataaac agctaaaaga tcacccgatt ctgctttcgg caaataagct tctttaatta
    38521 ccacatcgcc tgattcacac atttttcctg caatcgtcac tttctctgtt ttttcttccg
    38581 acagttttgc tgctaaatca caatcgtatt ctgcctgata cagagctgga cggatattat
    38641 ctgtcatccc gccatctaca aagtaatatt ttttatgtgg tgtctttttg atgaaaccaa
    38701 tcgtgtatag tgttgtacca gcttctccaa caatacttct acccggttcg atcagtaatt
    38761 gacgaatggt cagttttcgt ttttccaaag cttcttccgt atattcaatc agttttttta
    38821 gtgcctcttc gactgggatc ggctgatcat tttgtgtata acgaatccca aaccctccgc
    38881 caagattcaa ggaaagaggt tggtcaaaat cggttaagta attcgtcaat ttgtcgattt
    38941 cagctaacca agcagtaaca tcaaaaattt gtgacccgat atgagcatga aatccttcta
    39001 actctagata gctgctttct tttatcaact gaagggcttg ttggcactct ttactttcat
    39061 agctcatgcc gaattttgaa tcaatatgcg cagtgacgat atattcatga gtatgtgctt
    39121 ctactccgac attcaaccga agcatgatac agattttttt taaatagcgt tggcttaatt
    39181 ctgctaataa ttcaacttcc atcaaattat cggcaacaat atgcaacaaa tcattttcaa
    39241 gtgcatagat cagttcctct ggcgtcttgt tgttaccatg gaagtagata gtatctctag
    39301 gaaaatcagc ttgcaaagcg gtatagattt ccccaccact gacaacatct agcccgaatc
    39361 catattcacg aactaaatgc agcatctcaa tcgtttggaa tgctttagag gcgtaaagca
    39421 cttttgtatc aaaacgtttc gatacaaagc ctcgcttgaa tgacagcatc gtttgtctca
    39481 tcttttcttc atcatacaca tacagaggtg tcttatactt ttctgccaat gtgcttgctg
    39541 tgactccgcc aattatcaat tctgtcattt tttcattcct cttttcactt tttttccgtt
    39601 tttattggaa tttcatttga taaagatttg aattttattg gaataatatt aaacaaaatt
    39661 ttctgaattg tctagtatat ttcgaaatat ttttttaaaa ataagaataa aaatggttat
    39721 aaaaacaaaa tacccgaact attagaagct atttatacat aaaaacataa attcaacact
    39781 atttagtgat gaaaaaatat tttcgcttca atatagttcg gaatagttcg gatattttca
    39841 attttatgaa tcatttcaat ttcttcataa ataagtgcag ttttttcttt ctctagcttt
    39901 tgccattcat ctttcaaaat ataaataaaa tcaaatactt ttttattttt caatttgctg
    39961 attccacaaa aaacctcttg aaaggcagat ccgatgatct gcctttcaag aggtcaagct
    40021 ctgttttcaa attcttatca atccgttagc gaattatttt gacgcacgtt ttacataaga
    40081 accttctgct gtattgattt ccaacaattc atctgtttcg atgaagtcag gaacattgac
    40141 tactaagcca gtttccattg tcgctggctt acctgaacca gtgactgttg cacctttgat
    40201 ggaaggttgt gtttccacaa cacgtaatac gacagttgtt ggtaattgaa cgccgatgac
    40261 ttcgctaccg aaaaattgga tttttacttc catgttttct aggatatatt tcatttcttc
    40321 ttctactgtt tctacaggaa tttcatattg ctcatatgtt tccaaatcca tgaagaaagc
    40381 tgtatcatcc atcgtgtaca aatattgtac aggttttgtg tcgatatgtg ctttttcaaa
    40441 tttttcttct ggtctgaacg ttgtgtcgta tgttgcacct gaacggacat ctttcagttt
    40501 catacgcata actgtatttc ctttaccagg tttatggtgg cttgcatcca gaactttaat
    40561 cagtttgccg tcttgtacga aagtcatacc ggctcttaaa tcacttgctg agatcattat
    40621 ttttgctcct tcttttcttt atcataatca actttattgt atcagacaac cggccagaaa
    40681 actagagtat tctctatctt ttaaggtctc atgcctattt gtcagaaaaa taaacacggg
    40741 taaacgatgc tatcgaaata tttttttctt tcaagccttt gacttttatt ttttttcatg
    40801 ctatcgttaa taaaaatttt acttggaggg atacacatga cagtcagcaa ccaaaaacaa
    40861 ttgaatcgtt gctgttgtat ggtgcataaa agcagcatac tatttgtcat gtctattaat
    40921 tgcccttcat agaatgagta ccaggtaact tatcttttta tctactcaat ttttctctat
    40981 gtctttcaag gagctgattt aatagtcagc tccttttttt attatctttt tttaggaggc
    41041 ccacatgatt atctcgacaa ttacagaagc tattggttcc acaccgcttt atcgctttga
    41101 caatcagcac tattctatgc ctaaaggttc tgctatttat gcaaaactag aatatttaaa
    41161 tcctggagga agtatcaaag atagattggg cagttatttg atcaaagaag gatttgccca
    41221 aggaaaaatc aatgaaaaaa cgacgatcat cgaaccaact gccggaaata caggaattgg
    41281 gatcgctctg gccgcactca catacaaatt aagaacggtt tttgttgttc cagagaagtt
    41341 tagcttagaa aaacaacaat tgatgaaagc attgggagca aagatcatcc attctcctag
    41401 tgagcaagga atcatcggtg ccatcagcaa aagtaaaaaa cttgcagagg agatatcaaa
    41461 cagctatctg cctcttcaat ttgaaaataa agataatcct gctgcttact atcatacgtt
    41521 agggccagaa atctttcatg aaatcaaaga aaatatccat agttttgtag caggaattgg
    41581 gagtggtgga acctttgctg gaacttctac ctttttgaaa gaaaaatatc ccgacattcg
    41641 aataatcggt gtagaaccag agggctctgt gttaaatggt ggtgatccag cccctcacga
    41701 aattgagggt atcggtgtag aattcattcc gccgtttctt tctccccttt caatcaatca
    41761 gatcgagaca atctctgatg tagaagggtt taactatact aggcagttag cacgagaaca
    41821 aggactatta gttggaagtt cgagtggtgc ggcttttgca gcagcattaa gagaaattcg
    41881 tcgtttacca cctggtcatc gtgtcgttac gatttttcca gacgcagcag accgctattt
    41941 atcaaaaaac atttacttat aggaggaaag aaacatgcac attcaaacaa aattgatcca
    42001 cggaggcatc agtgaagatc ccacaacagg agctgtcagt gtacctattt atcaaacatc
    42061 cacttatcgg caagatggcg tcggccagcc taaacaatat gaatattcaa gatctggtaa
    42121 tcctactcgt tttgcattgg aagaactgat tgctgatttg gaaggcggtg ttcgaggatt
    42181 cgctttttct tctggtcttt ctgggattca tgccgttttt tctctttttc aagctggcga
    42241 tcatatctta ttaggagatg atgtctatgg aggaacattc cgtctatttg acaaagttct
    42301 tactaaaaat ggtctcgagt acacgatcat agatacgagt aatcttgata aaatagaaca
    42361 atccatcaaa cccaatacaa aagcacttta tcttgaaaca cctagcaatc cgcttctaaa
    42421 gattactgat ttagaaaaaa gtgccactct ggctcatcaa catggattga tagttattgc
    42481 tgacaataca tttgctactc cttattttca aagaccttta gatctcggtt ctgatatcgt
    42541 tgtccatagc ggtaccaaat atctaggcgg acacagcgat gtagtagctg gtttagtgac
    42601 ttctaaccat aaagatctag ccgatcagat cggattttac caaaatgcta tcggagccgt
    42661 acttggtccc caagatagct ggttacttca gcgcggaatc aagacactaa gtgttcgtat
    42721 ggaggaacat caaaaaaatg cttttgtggt agctgatttt ctattttctc atccagcagt
    42781 agaaaaggtc tactatccag gattacccga tcatgaactc catggagttg ctaagcagca
    42841 gatgagcggc tttagcggaa tgatttcttt tactttaaaa aatgaagaat ctgctattcc
    42901 atttgttgaa tcactccaac tatttacttt agcagaaagt ttaggtggcg tagaaagttt
    42961 agtggaaatt ccttctgtca tgactcatgc gtctattcca aaagaaaaac gagaagaggc
    43021 tggcataaag gacggcttga tccgtctttc tgtaggaatt gagtatggtc aggatctcat
    43081 aaacgattta gctcaggcat ttgacagaat caagaactag aagtcccaat aaacgactta
    43141 gcagaaaaat ctaaaaaata tttcagattg ctacaccttg gaaacgataa gtgttagaat
    43201 caagtagaaa gacgtttttg taaatttcac taaataattc tttagtgaaa agcatcaaaa
    43261 cgaatgaaca aacagatagg agtcttacaa atgagaatga atgtcgaaag ttttaatctt
    43321 gaccatacaa aagtcaaagc accttatgta cgtgtagctg atcgaaaaac gggtgcgcat
    43381 ggcgatgtca tcattaaata cgacgtccgc tttaaacaac caaataaaga acacatggac
    43441 atgccaagtc tacattcact tgagcacttg actgccgaac taatccgcaa tcacgcagat
    43501 tacatcgtag actggtctcc aatgggatgc caaacaggct tttacttgac tgtattgaat
    43561 catgaaagct acgaagacat tttagatgtt cttgaagcaa caatgaaaga tgttcttgaa
    43621 gctactgaag tacctgcaag caatgaaaca caatgtggct gggctgccag ccatacacta
    43681 gaaggtgcac aagctttggc tcgtgaattt ttagcaaaac gcgatgaatg gtctgaagta
    43741 gaagcatagt ttctaaaaga acgtaaagat gagaagcaat atagctatct tatcttcgta
    43801 ttttattaaa tatttattgt ctgaaaagag ttctaaacgg gaaaacaaat agctagtttt
    43861 cagtttagcg ctctttttat tatataaaat agaaaggaga aaactttatg tctatttata
    43921 attttgaaac tactttagaa gacggcactg tctattcatt gaaaaaatat gcaggcaaac
    43981 cgataatcat tgttaacact gcaacaaaat gcggtctagc acctcaattt aaacaattag
    44041 aagaacttta ccaagcttat cagacacaag gtctggttat cttaggcttt ccttccaatc
    44101 aatttaaaca agagctagat tcagcgactg aagcagcaga agcttgtaaa accacttatg
    44161 gcgttacctt ccctatgcat caattgattg acgtaaatgg agaaaataca gatcctcttt
    44221 ttacttatct taaaaatgaa gctcctggta ctgtcactaa agcaataaaa tggaatttta
    44281 ctaaattttt gattgataga aatggcaaag taatcgaacg atttgcacca caaacttctc
    44341 cattgaaaat gaccgctgca attgaatccg ttctttaact taataaaaat aaaaaaagat
    44401 gaactatttc tagttcatct ttttgccttt gcgtggcgac gtcctactct cacaaggggc
    44461 aacccctcac tacaatcggc gctaagaagc ttaacttctg tgttcggcat ggttacaggt
    44521 gtatccttct cgctatcgcc accacactgt ggtgttatct tttattgagt aaattttgtt
    44581 cactcaaaac tggatttgaa gtca
//
LOCUS       DACCKU010000014        43087 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.014, whole genome shotgun sequence.
ACCESSION   DACCKU010000014 DACCKU010000000
VERSION     DACCKU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 43087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.014"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(177..1214)
                     /locus_tag="IVN13_000707"
     CDS             complement(177..1214)
                     /locus_tag="IVN13_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7011221.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1661..2644)
                     /locus_tag="IVN13_000708"
     CDS             complement(1661..2644)
                     /locus_tag="IVN13_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP7011222.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSSAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(3057..3827)
                     /locus_tag="IVN13_000709"
     CDS             complement(3057..3827)
                     /locus_tag="IVN13_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011223.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(3820..4755)
                     /locus_tag="IVN13_000710"
     CDS             complement(3820..4755)
                     /locus_tag="IVN13_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011224.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(5071..6720)
                     /locus_tag="IVN13_000711"
     CDS             complement(5071..6720)
                     /locus_tag="IVN13_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7011225.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAASFDEKRQWHVLG"
     gene            complement(6915..7811)
                     /locus_tag="IVN13_000712"
     CDS             complement(6915..7811)
                     /locus_tag="IVN13_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7011226.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNRWVDENEQKGQVFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(7804..8484)
                     /locus_tag="IVN13_000713"
     CDS             complement(7804..8484)
                     /locus_tag="IVN13_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011227.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILMDKESRDVNVNGVTVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(8485..9267)
                     /locus_tag="IVN13_000714"
     CDS             complement(8485..9267)
                     /locus_tag="IVN13_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7011228.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(9260..10177)
                     /locus_tag="IVN13_000715"
     CDS             complement(9260..10177)
                     /locus_tag="IVN13_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7011229.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(10180..10395)
                     /locus_tag="IVN13_000716"
     CDS             complement(10180..10395)
                     /locus_tag="IVN13_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011230.1"
                     /translation="MSTITTITMVASPLGLALFGALFDWQTTQPRLRDSLIFLEIQLE
                     NLYFLYTFLAAFFIPVNYTSLINKKEG"
     gene            11251..14478
                     /gene="fss3"
                     /locus_tag="IVN13_000717"
     CDS             11251..14478
                     /gene="fss3"
                     /locus_tag="IVN13_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP7011231.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            14590..14904
                     /locus_tag="IVN13_000718"
     CDS             14590..14904
                     /locus_tag="IVN13_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7011232.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            14917..15291
                     /locus_tag="IVN13_000719"
     CDS             14917..15291
                     /locus_tag="IVN13_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP7011233.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            15301..15645
                     /locus_tag="IVN13_000720"
     CDS             15301..15645
                     /locus_tag="IVN13_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP7011234.1"
                     /translation="MEINVEHILDCLDQFGKGELTGEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            15714..16730
                     /locus_tag="IVN13_000721"
     CDS             15714..16730
                     /locus_tag="IVN13_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP7011235.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            17324..19198
                     /gene="ltrA"
                     /locus_tag="IVN13_000722"
     CDS             17324..19198
                     /gene="ltrA"
                     /locus_tag="IVN13_000722"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7011236.1"
                     /translation="MNVETDEVQGSGKNVGKSVMEVPRLNTKIRYWEYYDLQETFDQL
                     FTQSRNGKKFYQLYELIISENNILLAYRTIKANKGSSTPGTDSFTIDNYKEMNQAEFI
                     HLILSHLENYKPKSIKRVMIPKPNGEKRPLGIPCMIDRMIQQMFKQILEPICEAKFYE
                     HSYGFRPLRSAKHALGRIMYLINISKMHYAVDIDIKGFFDNVNHRLLIKQLWNIGICD
                     KQVLAILSKSLKSPIQGEGIPSKGTIQGGIISPLLSNVVLNDLDHWVSKQWHTFETKY
                     PYTKGYNKFRALRDTNLKQGYIVRYADDFKIMTNDYPTALKWFHAVKLYLKDRLKLDI
                     SNEKSKIVNLRKCKSEFLGFAICVKQKGKKWVCNSRISNKKKDQIKEEIKQRIKDIQK
                     SPTAQNALLFNALVLGVHNYFRYATHINLDLNRMNYVLSFTTYNRLSNCSTRCYPNKA
                     SPTYKKFYSTTMKTYKVAGVYLYPLCDVSTKTIYGFNTEDTPFTPFGRQRLEHKSLQS
                     LVFQELRKLMESKIPNRTVEYLDSRISRYSMKSGKCEITKQFLPAKAVHCHHYLPKSL
                     GGDDKFDNLRIIHKDIHLLIHTTNKMIIDHYVHELKLVPEQIAKINLYRKMCNLQNIQ
                     "
     gene            19324..19680
                     /locus_tag="IVN13_000723"
     CDS             19324..19680
                     /locus_tag="IVN13_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAP7011237.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            19725..20867
                     /gene="dcm"
                     /locus_tag="IVN13_000724"
     CDS             19725..20867
                     /gene="dcm"
                     /locus_tag="IVN13_000724"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP7011238.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            20857..21146
                     /locus_tag="IVN13_000725"
                     /pseudo
     CDS             20857..21146
                     /locus_tag="IVN13_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21402..22586
                     /locus_tag="IVN13_000726"
     CDS             21402..22586
                     /locus_tag="IVN13_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP7011239.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            22601..22696
                     /locus_tag="IVN13_000727"
     CDS             22601..22696
                     /locus_tag="IVN13_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP7011240.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
     gene            23466..25376
                     /gene="ltrA"
                     /locus_tag="IVN13_000728"
     CDS             23466..25376
                     /gene="ltrA"
                     /locus_tag="IVN13_000728"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7011241.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESSEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
     gene            25480..25704
                     /locus_tag="IVN13_000729"
     CDS             25480..25704
                     /locus_tag="IVN13_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011242.1"
                     /translation="MSGTYLSIYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFL
                     VIALIAVGLVFNASGVKDVLLNLFNRIVGA"
     gene            25717..26220
                     /locus_tag="IVN13_000730"
     CDS             25717..26220
                     /locus_tag="IVN13_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP7011243.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            26347..27384
                     /locus_tag="IVN13_000731"
     CDS             26347..27384
                     /locus_tag="IVN13_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011244.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            27399..29096
                     /locus_tag="IVN13_000732"
     CDS             27399..29096
                     /locus_tag="IVN13_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAP7011245.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKFPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            29089..29550
                     /locus_tag="IVN13_000733"
     CDS             29089..29550
                     /locus_tag="IVN13_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011246.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            29563..30423
                     /locus_tag="IVN13_000734"
     CDS             29563..30423
                     /locus_tag="IVN13_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011247.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            30461..30850
                     /locus_tag="IVN13_000735"
     CDS             30461..30850
                     /locus_tag="IVN13_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7011248.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            30837..33284
                     /locus_tag="IVN13_000736"
     CDS             30837..33284
                     /locus_tag="IVN13_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7011249.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            33289..35397
                     /locus_tag="IVN13_000737"
     CDS             33289..35397
                     /locus_tag="IVN13_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7011250.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            35394..36398
                     /locus_tag="IVN13_000738"
     CDS             35394..36398
                     /locus_tag="IVN13_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP7011251.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            36417..37328
                     /locus_tag="IVN13_000739"
     CDS             36417..37328
                     /locus_tag="IVN13_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7011252.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            37515..37802
                     /locus_tag="IVN13_000740"
                     /pseudo
     CDS             37515..37802
                     /locus_tag="IVN13_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(37990..38205)
                     /locus_tag="IVN13_000741"
     CDS             complement(37990..38205)
                     /locus_tag="IVN13_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011253.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(38464..38631)
                     /locus_tag="IVN13_000742"
     CDS             complement(38464..38631)
                     /locus_tag="IVN13_000742"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7011254.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(38976..40415)
                     /locus_tag="IVN13_000743"
                     /pseudo
     CDS             complement(38976..40415)
                     /locus_tag="IVN13_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(40963..41634)
                     /locus_tag="IVN13_000744"
     CDS             complement(40963..41634)
                     /locus_tag="IVN13_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP7011255.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(41983..43029)
                     /locus_tag="IVN13_000745"
     CDS             complement(41983..43029)
                     /locus_tag="IVN13_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7011256.1"
                     /translation="MTRPNHIESSKKEAPKTEGQTKNFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
BASE COUNT        14006 a         7820 c         8486 g        12775 t
ORIGIN      
        1 aatcattgaa ttacagaaca ccaatcgaaa tatttttgag ctatgtacaa gaagcatttt
       61 attctagctt aatttgacaa atcataaaat aaaaaaacaa gagcttatgg caaattactg
      121 tcatgcgctc ttgtttttat caaagtcaaa taagaaaaaa atcggcgtat tcttgcttat
      181 ccgttgaacg gaccactaac aacgactttt cctaacatat gtcctgtttc tacgtctttg
      241 gttgcttgtt tcaagttctt cgcattcatc ccatctgaat agaccttgct caatgtactg
      301 cggataatcc cttcatttgc taaagcagca atgtgcgcta aagcttctcc ttgcgtttcg
      361 atttcatagt tatagtctgt tttagcgaac atatactccc agtcgaagct gatagattgg
      421 tttttccaaa cacctatatc taacggctct ttgatcccta cgatcgttcc cacatgtcca
      481 aatggtcgga caaggtgctt gatttgatca aagtagcggg taatatcgaa taatacagcg
      541 acatactcta cttgatcgat tcctaattca cgaaaacttt tacttaagtc gtgatgataa
      601 tcgattggat agtctacacc tgtttttttc agccattcaa aattcttcgg acttgcagtc
      661 gcgtagactt ctaggccagc ccaatgcgct agttgattca aaatcgaacc gacgcccccg
      721 gatccgttga tcactaagat ttttttcccg cgattggcat tttcttctgg agttagacca
      781 aatttttcaa ataacaattc ataagcggtc aatgaggtca acggcaaggc tgcagcttct
      841 tcatctgaaa gattttttgg tgcaagagcg acaatacgtt cgtcaactag ttggtattct
      901 tgattgcttc ctgctcttgt tgttgtacct gcgtaaaaaa cgcgatcgcc gacagaaaat
      961 ttttgcactt cgtcacctac agcaacaact ttcccgactc catcaaaacc taagatacgt
     1021 aaagcatttc ttatcccgtt atcttggcga agtttcgtat cgacaggatt aacagatact
     1081 gcgttgactt ttaccagaag atcccgtgaa ctgggtacag gtatctgttc tttttcatca
     1141 ataaagcttt ttggatcatc gatcggcaac ccttcataaa aaccgactac cttcattaat
     1201 tccttttctg acatacgaat tcctccttag cttcatcaag ttggagcatc catacagtgt
     1261 tcatttcaat atatatagta tgctatttgt tccacctttt ttattcatca taattattct
     1321 cttacttaaa gtaaactatt tgcacttttt tggttaaaaa agaggttgcg acaagacttt
     1381 tgtcacaacc tcttataaac ggtgttctcc taccaactct tcggtcacaa gtcaaaatat
     1441 tcatcaaaca tgagaagctg ttttttgaaa ttgctccttg tgactcagag ttaaacggca
     1501 ggctcacaac ctctgatata aacggtgttc aatcaactga ccctcggtat tagctcaaaa
     1561 aacgacaaaa tatgaagagc tatttttgtt tggctgttct tattacttga tgcaggacag
     1621 ctttttcacg acctctaatt cacggtgttc aagcaagaaa tcattgttca ctgaaaagcg
     1681 ttggatgttc tttttcaaaa gtgtctaaga tctctcgtac ttcatcagta gatttcgtat
     1741 tcatcaactg aactcgcaag tcatttgctc ctggaaatcc tttgatatag attttaaaga
     1801 aacgatgtag tcctgtgatg gaacgcggga tgatttctga ataatgatct tgcagatcta
     1861 gttgcagacg aagtagtccg atcagttctt tttctgtatg ttccctcgga tctttttcaa
     1921 atgcataagg attcttgaaa atccctcggc caatcatgat gccatcaacg ccatatttct
     1981 cggccaattc ttctcctgtc tggcgatcgg ggatatctcc attgatcgtg attagtgttt
     2041 gtggagcgat ttggtcacgt aaagcaatga ttttaggaat gacttcccaa tgtgcatcta
     2101 ctttactcat ctctttacgc gtacgcaaat ggacagaaag attggctata tcttgatgaa
     2161 gtaggtgact gatccattct tccatctcag acatttccgt atagccgata cgcgtcttga
     2221 cgctgacagg caaaccgcca gctttagctg cttcaatcaa ttgtgccgct acttctggac
     2281 gaaggatcaa gccactgcct tttccacgtt ctgctacatt agggactggg cagcccatgt
     2341 tgatatcgat tccttggaag cccatttctg ccatagcact gctcatttct ctaaaaaatt
     2401 ctggcttgtc tccccaaata tgggcaacca tcggctgttc atcttctgta aatgttaatc
     2461 ttcctcgtac actgtctttt ccatctggat gacagaaact gtctgagttt gtaaactcag
     2521 taaagaagac atccggtgct gctgcatgct ttacgacatg gcgaaataca acatctgtta
     2581 catcttccat tggtgctaaa acaaaaaaag gctttggcag ctctttccaa aaattagtag
     2641 tcatttattt gttactctcc attctatcaa gcagtgaagt cgcaatcttt ctcaaaaaca
     2701 ttgtcattat actaggaaac aaaaaaagaa gcaaagtgaa cagctcagaa ggtttgtctg
     2761 attgaacaaa aagaggactg tgacaaaaat cttcacagtc ccttttccgg aataaatggt
     2821 gttctcctgc caactcctcg gtcacaagtc aaaatattca tcaaacatga gaagctattt
     2881 ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc tccactaaac
     2941 ggtgttcaat cagctgaccc tcgatattcg ctcaaaaaac aacaaaatat gaggaactat
     3001 ttttccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc tcttttctaa
     3061 cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa aacaacgatg
     3121 aataacacaa gtacgatcat cgatgttgct actggcacat cgatcgttcc aaggaatccg
     3181 taacggaaac cggagatcat ataaacgatc ggattgactt tcgaaacagc ttgccaaaag
     3241 ggcggtaaca tagagatggc atagaaaacg ccacctagat aagttaacgg ctgcaagaca
     3301 aatgtaggta cgatcgatac atcatcataa gactgagcaa aaatcccgtt gagcagtcct
     3361 gctaacgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa ccatgaatac
     3421 acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac gagaatactg
     3481 cgacccacac caccaatcac aaagccccaa ataatcacat gcgtaggaac aggtgctacc
     3541 aagatttctt cgatattttt ctgaaatttt tgagagaaga aggacgaaga aacattggca
     3601 tacgaactag tgatagcaga catcataatc aaaccaggaa cgatgaattc catatatgaa
     3661 aaaccaccca tgtcaccgat ccgcccaccg atcatttttc cgaaaataac aaaatacaag
     3721 gatgtcgtga tcactggcgg aaccagtgtt tggacccata tacgcaaata gcggttcgtt
     3781 tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt tctcctccgt
     3841 atgattattt tcttctgtga ttttcaagaa cagttcttct aaacgattcg acttattcct
     3901 catagaaaga actttgattc cttgtcttgt aagctgatcg aagattccgt tcaccccttg
     3961 atttcgttca acttcgacag cgattgtctg ctcatcttca aatgtacaag catatccttc
     4021 aatcacaggt ttggtatcat atggcgctaa atcaaagata aatgtttcaa attgcagttt
     4081 tgacagaagc tctttcatac ttgtattctc gatcagttca cccgattgga tgattccgat
     4141 gttgcgacaa agcatttcag cttcttctag ataatgcgtc gtcaaaatga tcgttgtacc
     4201 attttcgttc aattctcgta gaaactccca catctcgcgt ctcaactcga tgtcaactcc
     4261 tgctgttggt tcatctaaaa tcagcaaacg tggttcatgc atcagtgctc tagcaatcat
     4321 cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct tttcccaaag
     4381 attggactgc ttcaaatatt tttcactgcg tttcatcgct tctttgcgag aaacaccgta
     4441 gtaaccggct tgattcacga cgatttgctg gaccgtttca aaagggttaa aattgaactc
     4501 ttgtggaacc aatccaatct gttgctttgc tcgaactaga tccttgtcca gatcataacc
     4561 gaagacattc acttttccag atgttttatt gactaaagaa gtaataatcc cgatcgttgt
     4621 agacttccca gctccatttg gccctaatag cgcatagaaa tctccttctt tcaccgaaag
     4681 atcgattccc cgcaatgctt cgaccccact tccatagatc tttttcaaat tatttatttc
     4741 aagcgcattt gccatttatt tcctatctcc ttatagacat gattctttat tgtatcatgg
     4801 aatacagcag atgaaaaaac aacttggttc cagcgaaaaa tcaggttgct gggctcagga
     4861 aaatctccta atgaaagaca tcaatcggac gaagtcagac aaccttttca gaaaaaacaa
     4921 atcgaagata aaggattact attcatatgt gcaacaaaag gagcgtgaga caaaagtaaa
     4981 gaacactttt gtctcacgct ctaaacacgt ataaacggcg gaagcatgag ccaagatttt
     5041 tgatccaata aatactttcg atcacttcgc ctatcccaat acatgccact gccgcttttc
     5101 atcaaatgac gcggcttccg tttttgcggt ttctactaaa gtctctgggt aatccaaaac
     5161 tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt tgaaatcaaa
     5221 actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac aataattctt
     5281 taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg gatactccgc
     5341 aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg ttccttttaa
     5401 aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca gcaaacgttt
     5461 gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat cttcgattgc
     5521 catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg ctgtttggat
     5581 cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt ttcctgatgc
     5641 attggaaccc gtgactaatg tattcttctg ccagttgact ggattcacta ccgcttcttt
     5701 taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact ctggtcggca
     5761 agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca attctcccag
     5821 caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga gaacgaaatg
     5881 ataggagata aatggtagca tgaacatgat gttgacatat tcaaaaatca attctgcttc
     5941 actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta atggtttagt
     6001 ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat tggcggctga
     6061 aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt gtttcttcac
     6121 tagataatag accgtattga atactaagcc accgattgct agatagattc ctagtacttg
     6181 acctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa taaataaacc
     6241 tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa aattatgatc
     6301 tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag gattttcctg
     6361 ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc tacgtaaacg
     6421 ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt caaatacttc
     6481 gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg aacggaattt
     6541 tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg gttgataagg
     6601 aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa aaatgtctaa
     6661 aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc tttggcccat
     6721 atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct atttgaaaag
     6781 ggggtacaaa aataactatc ttcgttacct aattcgtatc aatcacacca aaataaaaac
     6841 accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat tcacttcagg
     6901 taaattagag ccaattaaac cctccgtaac ttgacggaga tggagaagat gcccccgtct
     6961 aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag gtaaagcccg
     7021 attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg ctggaagact
     7081 ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa tcgaatgaat
     7141 ggatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa taaattctgt
     7201 atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt tgtatttatc
     7261 gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata aaaagagaaa
     7321 aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc tttttgcttc
     7381 gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct tgcaacagat
     7441 tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg ttccagtaat
     7501 tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag gccgacgatc
     7561 gatagtctga tgttttcttc catttcttga aatacttgtc ctttttgttc attttcatcc
     7621 acccatctat tcagtgcatc tactaggtcc attagattct tttctcgaaa ttcgactgaa
     7681 agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt taatcgcttg
     7741 atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag aagcaacagc
     7801 cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag catgtgcctt
     7861 tgctagcttc cctcgaagat tggaaatatg catattcact gttttctcac tatcaaaata
     7921 tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt tcggatgttc
     7981 taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagtca caccattcac
     8041 atttacatcc cttgactctt tgtccattaa gatttccttg taatagatgc gttgcgacag
     8101 atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta acaacagttc
     8161 ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca aggaaacttt
     8221 atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg tctgtcggat
     8281 ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa ggatcaaatc
     8341 gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat caagaagttt
     8401 gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt cttcgataat
     8461 caaaatagtt tccagcagga ccacctactt aatgtcagat ttttggaaaa taaacattcc
     8521 tagagctgat gataccacga ttgttactag actgaccaga atcattttcg gtgcttgcga
     8581 ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa accactccgt
     8641 tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg ttcccatgat
     8701 ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg tcaaagccac
     8761 tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt ttagaacata
     8821 atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg tgtaagcaat
     8881 catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg taacgagaag
     8941 tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc gaatcgtacc
     9001 gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca gaagaatcca
     9061 tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg tatccgttgc
     9121 ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag aataaaaaag
     9181 cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc atacttttat
     9241 tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt aagaaataat
     9301 cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc tctaccaatg
     9361 cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca cggctagatt
     9421 ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt tccgtcgccg
     9481 aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc gataattctt
     9541 tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac aattcatcta
     9601 gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt tggatgattt
     9661 ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc aaaatcaaaa
     9721 attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata cctaaagaga
     9781 aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc aggtcttcga
     9841 tttctctatc cgacacaccg ccgttcgctg cttggatacg taagttatct cttgcagtta
     9901 attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt gctgcctgca
     9961 aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa cccataatag
    10021 ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag atcattccac
    10081 gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt ttggttatac
    10141 cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt tcttattgat
    10201 aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa gaaagtataa
    10261 attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt gagttgtctg
    10321 ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca tggtaatcgt
    10381 tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct gaagctagac
    10441 catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa aataatacat
    10501 gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga cttttcacaa
    10561 tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatctt
    10621 catcaaacat gagaagcgat ttttgtaaat tccttcttat ttcttgaagc tgaccacttc
    10681 tgtcacaacc tcagaagcgg ataaataata ttgtttgaag atacttgatc tttttcccgt
    10741 ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca tagctaaaaa
    10801 cacaaaaagg cactttccaa attgtggaaa gtgccagaaa agttaggatt aaggtaactg
    10861 cgctcagcat tgcttcgccc agtacgattc tcgtaactcg tgaactcgaa cgataatcgc
    10921 aattaacgtt ttgagaactg tgaagcttta cgggctttct taagacctgg ttttttcttc
    10981 gtatcattga ataagtttga aaagccttga tttataaagg tttttccgta aatatatttt
    11041 gttatatttg attaaatttg aaatgtttgt gttctaaaat gtgttctaat ttaaaggcag
    11101 tatatttcgt attttatatt aaatttttag gctcattcat tctcaaatcc attctattta
    11161 gtcaataaat attttaaaaa gactttataa acaaagatgt tcaagcgagc atcttatttt
    11221 tttgtgccta atttgaaagg agaacatttc atgtataaaa aacaaaaaaa gaaattatgt
    11281 gtattaacta ttttgagttt ggctgttcaa tcaattgtag gagcaattgc tcctacgatt
    11341 gcttttgctg atgaaattac ccatccacaa acggtaaccg tagaactaga tttagcacat
    11401 caatatgctg ttgaaggaac ttttagtgat ggtcgtccaa tgtcagaagt cactgttccg
    11461 cattatgcag tgtacaacgg cgttaaacaa gatattttct gtgttgaacc aggagtcccg
    11521 atttacaatg agttcactcc tggatatgag aagaacccgt taccagatat gtccgataaa
    11581 gccaaattag tttccgtttt atggaaaaaa gtaggtacag atgcagatac tcatattgtg
    11641 gcacagaaga tgatctggca agaagttaat gggtataccc ttcactcaat aaaacgatca
    11701 gatggtagtg ccgtaaatat cgcagccatt gaggcaaaaa tcaatcaagc cattgccgat
    11761 tatcagaaga aaccaagctt ccataatagt acggctaaaa cagtattagg gcaatcgact
    11821 actgtgacgg atacgaataa tctgaatttg tcagagttcg atgaggttgt ggaaaatacc
    11881 gcaaacattg attatcgtgt gaatggcaat cagttgataa tcagcccaaa tgccaattca
    11941 aaagaaagtg gtgtgttgac gcttaagaaa tctgctggta ccggaacacc tgtcgcctat
    12001 aaaaaggctg gccaacagac tttaatggcc ggggcgatcg ataagccgaa cacttatacg
    12061 gtcaaaattg atgtggaaat ggagggttct ttgaagatta aaaaagttga taaagaatcg
    12121 ggtgatatta taccaggaac ggttttccat ttagactttg gaaaaacttt acccgcaaaa
    12181 gacgtgacca ccgataaaga aggcattgcg accttagatg agattcctca tggtacaaag
    12241 gtaaccatta ctgaaaaatc ggtgccggcg ccttatacga ttgataccac acccatgact
    12301 gccacaatta aagcaggtga aaccatttca gtgacttcaa aaaatacgcg agaaaaaggt
    12361 caaattattc tggataaaac cggaattgaa acagggagtg atctttggaa cgataattac
    12421 tctttagcgg gcaatacgtt tgccattcgc aaggatagcc caactggtga aatcgtccaa
    12481 gaaatgacca cggatgaaaa aggtcatgca gaaacaccaa aagagattgc caatgcgttg
    12541 gaattgggaa cctactatgt gactgaaacc aaggctagtc atggctttgt gaatactttc
    12601 aaaccagtaa aagtcgaatt gaattatgtc aatcaaacag tggctcttgt taccagtaac
    12661 gtaaaagggc aaaaccaaga aattactggt gaaaccactt tgacaaaaga agacaaagat
    12721 accggtcatg aaactcaagg gaaagctgag tttaaaagag ctgaatacac cttgtttact
    12781 gttaaggatg gaaaagcggt caaatggacc gagaacttta aaccagaatt gttgaaggga
    12841 acgaaagctt ctgatgaaac cgtaactttg actttagatg aaaagaatca agttgccgtt
    12901 aaacacttag cgattaatga gtattactgg caagaaacca aagcgcctga aggatatacc
    12961 ttggatgaaa caaagtatcc tgtatccatc aaaaaggtgg atgatagcga aaaaaatgct
    13021 gtgattactc gagatgttgc ggcaaaagaa caagtgattc gctttggctt tgatttcttt
    13081 aaatttgctg gatcaactac tggcactgcc gaaactggct tcaatgatct aacctttaaa
    13141 gtgtcgccat tggaaggaac caatgaaatc accggtgccg aagatgaagc gaccacagct
    13201 tataacgagc aattagggtt tgatggttat ggcaagtttg aaaatcttcc ttatggggat
    13261 tatttacttg aagaagtaga agccccagaa ggttttcaaa agattacgcc attagaaatc
    13321 cgttctactt ttaaagaaaa taaagaggat tataccaaga gtgattatat ctttactatt
    13381 accgaaaaag ggcaaaagca accgattaaa acggtaacgg tcccttatgg gaatctaacc
    13441 aatgagaatt tctcagtcag tttgaaccgt ttgatgcttt atgatttgcc tgaggaagaa
    13501 gatagcttaa cttctcttgc gacttggaaa gaaggagata aagaattgac tactcttgat
    13561 tctaccgagc tagtcgataa attgagctat aatctacatg aaatcaaaga agactggtat
    13621 atcgtagctc aagccatcga tgtagatgct acaaaagctg cccaagaaaa agataaaaaa
    13681 gccaaaccgg tggcgattgc ggaaacaacc gcaactttag cgaataaaga gaaaacagga
    13741 acttgggaga ttctgcataa attaactgtc gaacaagttt tagacaagac cattgtctta
    13801 ttcaactatg tgtatgaaag caaagaagcc tatgaagctg gtgatgagcc ggtagcgaag
    13861 gatgctagct tgaacaatca agcgcaaact gttaattgta tggtggaacg tcatgtttcc
    13921 atccaaacca aagcgcactt agaagaaggt tctcagacct ttactcatgg tgacgtggta
    13981 gatatgtttg atgatgtatc cattacccat gatgtactag atggctcaaa agaagctttt
    14041 gaaacaattc tgtacgcttt atttccagat ggtacaagta aagaaatttg gaaatctggc
    14101 aagattgatt acgaagtgaa tgacaaagaa tttaccaaaa ccgtacttgc ggaaaaagtg
    14161 gatacaagta aatatccaga aggtacaagt tttaccttca aagaaattaa ctatgacaaa
    14221 gatgggaata tcaatggaaa acacaatgaa gatttgaaag aaaaatctca aactttaacg
    14281 cccaaaaaag ttccgacaac accaagtaca ccagaacaat ctgaaatgcc aactgttcca
    14341 agtggctctc aagaatctag tcctacagtg aaggcattcc ctcaaaccgg ggagaaaact
    14401 tccaatgtgc tactgttcat tggctttagc ttgatctttg cgacggctgg ctattatttt
    14461 tggaatcgtc ggaactaagg tgataagatg aaacatgtaa atagtagaaa acaatacgcg
    14521 cgaccgcctc ctaaaaaagt ggtcgcgcta tttttgtatt caaaactaac aatgaaaaga
    14581 ggaaaaaata tggaattgaa atttgttgta ccagatatgg tcgagacgtt cggaaaacta
    14641 acatacggtg gtgaaggtga agttctatcg gaagggtatg gtcgaaatac tacaattgtt
    14701 ggtcgaagtt accatctgtt ttcaaataaa caacgggccg atgatattga agtggtggta
    14761 gctgcggaag ccggcgaaaa ggattttgac caagatcaac cactaaaagc tgtaaatccc
    14821 cacttaattg ccaaaggcta tgaaattgag aatcgtgggt ttaccgatta cgtgttgtat
    14881 gttgatgatt tagtgaaagc ataggaggaa aagaagatga gattaacaga aggtattgtc
    14941 gtagattcag gattaacctt tggcaagttg cgtttttccg cattacgtcg agaagtacgc
    15001 aagcaaaatg aggacggaac ggttagtaac gaggtaaaag aacggaccta taatttgaaa
    15061 tcctccgcgc aaggtcgaat gatccaagtc agtattccag caaatgtacc cttacgcgag
    15121 tttgcttatg atgcagaggt agagctggtc aatccaatcg tggatacggt cgccaattat
    15181 gtttttcggg aaggaaccac cgttaattgg tttatcaagg cagatgattt agtcttgaaa
    15241 cgacaactga atcaaggaaa acctacgaac caaaatgaag gaaagaaata ggtgaagaat
    15301 atggaaatta atgttgaaca tattttagat tgtctcgatc aatttggcaa aggtgagcta
    15361 acgggggaac aactcactaa cgcattaacc tatgatgaaa aaatgtttct gattatgcat
    15421 caagggttac tggaagtagg taacgatgcc aaagaagact ttgatgtact ggattggtta
    15481 ggggaagaag agtctttctt tatggttgtc gaggtaaatg aaagtttatg tcggcaggca
    15541 gaatctgttt tggaagaaat cgatgtggag atgccggatg ccatcgaaag atttttaaaa
    15601 caattagtgg aaacgaaaca acttcccgta gtagtaaatg actagcataa ctttttgaaa
    15661 ttgcccaatg aaatcctatt gggctttttc gactggaggt ggaaaccatc aaaatgtaca
    15721 aaggacatcg catacgcgca ggagatcaac acttgattta tcactttgtt cttggctggc
    15781 taatcttttt gttcattggt tggatgagcg tcttttattt acaagaactt agacaatttg
    15841 atatttctaa ggtatcattt tccactagtg aaattggctg gtctatgaag gatgtaatct
    15901 gcttgctagg aagtctcgct ctttcaggaa gcatgatgtt actttatata cacttttttc
    15961 aggatcattg gcgaagtctt tggcatcgac aaaagttagc tcggatgatt ctagaaaacc
    16021 attggtatga agtaaagcag actcaaggtg aaggtttttt caaagatttg aatagtagtt
    16081 ggaccaaaga gagtatcagt tacttcccca aaatttatta tcgaatgaaa gatggcttac
    16141 tttctattcg cgttcaaatt tcactgggaa aatatcagga tcagctattg aagttggaaa
    16201 ataagttaga aagcggactg tattgtgagt tagtagaaaa agaactcaaa gactcctatg
    16261 tggaatacac cttgttatac gatatgattg ccaatcgaat tgggatagaa gaaatagtgg
    16321 cagaaaatgg ggcgttgcga ttgatgaaaa atcaagtatg ggcgtatgat tccttgccgc
    16381 atatgttaat tgctggcggc acaggtggtg ggaagaccta tttcttactc accattattc
    16441 aagcactatt gaagtcagat gcagaattgt ttatcctcga tcccaaaaat gcggacctcg
    16501 ccgatttagg tacgataatg cctcacgtat actcccaaaa agaagaaatt tctgaatgtg
    16561 tggaagattt ttacgaacgc atgatagctc gtagtaggtc aatgaaagaa atgcccaact
    16621 acaaaacagg agagaattat gcgtatctcg gacttccgcc caacttttta atctttgatg
    16681 aatacgtggc ttatatgggt gcgaatcgtt ttcctacaag catcgaatag atgtgaaatg
    16741 gaagcctatt gagattatca atagtgacaa ctaatgtttt gagagatgga atgaaagcct
    16801 tcaagttgag ctgaaacatc cccgctaata ctcctacatg ctctaatcaa tgaagtagat
    16861 tggttttggt atgaagctag gtgaagtcgt ctgaaatctg cctaagtgct tgagcataag
    16921 gctgactata gggcgggcgg ctgaaaaaga taactatggt aagaatgtct gaaaatgact
    16981 gacgaatctg cgaatgtacg ggtctaaaag gctgatctta ttgaaagata ggaacatacg
    17041 gacgtatggt gagataccga caagtaaggt tccatgatat gaaaatcggg atagctaacg
    17101 aagaagctat aatttcacag gctcatagta gatacctaag gttatacatg atagctaaaa
    17161 acattggaac gatgtaagca gaaaactatc aattaaatga ctacttctag ataggtgcat
    17221 ataagtaata acgaaattgc tttgtttctg tgaaagtaag ggcacagtac cgatgaagcg
    17281 tctaacaaat gtggagggat agccctaagt ctatttaata acaatgaatg ttgaaacaga
    17341 tgaagttcaa ggatctggta agaacgtggg aaaatccgtt atggaggtgc cacgcttgaa
    17401 cacaaaaata cgttactggg aatattatga tctacaagaa acattcgatc agctatttac
    17461 tcaaagtaga aacgggaaaa agttttatca attatatgag ttaatcattt ctgaaaataa
    17521 tattctttta gcataccgta ctatcaaggc aaacaagggg tcttcgacac caggaacgga
    17581 ttccttcaca attgataact ataaggaaat gaaccaagca gagtttatcc atcttattct
    17641 cagtcattta gaaaattaca aaccaaaatc cataaagcga gtaatgatac ctaaacccaa
    17701 tggtgagaaa cgcccattag gaattccatg catgattgat cgaatgatcc aacagatgtt
    17761 caagcagata ctggagccaa tatgtgaagc taagttttat gaacatagct acggtttcag
    17821 accattgaga agtgcaaagc atgctctagg tcgcattatg tatctcataa acatcagtaa
    17881 aatgcactat gcagtagaca ttgatatcaa aggtttcttt gataatgtaa accaccgttt
    17941 actgataaaa caattatgga atataggaat ttgtgacaaa caagtcttag ctattctttc
    18001 aaaatctctg aaatcaccca ttcaaggaga aggtatcccg agcaaaggaa cgatccaagg
    18061 aggtatcatt tctccactat tatctaatgt tgttctaaac gacttagacc attgggtctc
    18121 aaagcagtgg catacctttg aaacaaagta tccctatact aaaggttaca ataaatttcg
    18181 tgctctaaga gatacaaatc taaagcaagg ctatattgtc aggtacgcag atgactttaa
    18241 aatcatgact aatgattatc cgacagcatt aaaatggttc cacgctgtca agctttacct
    18301 aaaagacaga cttaaattag atatttccaa tgagaagtct aaaatcgtca atttgcgtaa
    18361 atgtaagtcg gaatttttag gattcgctat ttgcgtgaaa caaaaaggga aaaaatgggt
    18421 ttgtaattca cgtatttcta acaaaaaaaa ggatcagatt aaagaagaga ttaaacaaag
    18481 aatcaaagat attcaaaaga gtcctacagc acaaaatgct ctcctgttta atgctctcgt
    18541 gctcggtgtt cataattatt tcaggtatgc cactcatatt aatttagatt tgaataggat
    18601 gaactacgtt ctttcattca ctacttacaa ccgattatca aattgtagta caagatgtta
    18661 ccccaacaaa gcttccccaa cttacaaaaa gttttactca acaacgatga aaacgtataa
    18721 agtagctgga gtctatttgt accctctttg cgacgtatct acaaaaacaa tttacggatt
    18781 taatacggag gatacaccgt ttactccgtt tggtcgtcaa agattggagc acaaaagcct
    18841 acagtctctt gtgttccaag aattaagaaa attgatggaa tcgaaaatac cgaatagaac
    18901 ggttgagtat ttagatagta gaatatctcg ttactcaatg aaatcaggca agtgtgagat
    18961 tacaaaacaa tttttgccag caaaagccgt tcactgtcat cactatttgc caaaaagtct
    19021 tggtggagac gacaaattcg ataacttgag aattattcat aaggatatcc atcttctgat
    19081 ccatacaaca aataaaatga taatcgatca ctacgtgcat gaactgaaac tggtgccaga
    19141 gcaaatagca aaaatcaacc tttatcgaaa gatgtgtaat ctgcaaaaca ttcaatagaa
    19201 aataaataga tggaacgccg tatgaggtga aagtctcacg tacggtgtgg agcgggggaa
    19261 aagatggaga ttatatcaaa ttcttaccta tcgctataga tgttaacgac aaaagaaagt
    19321 gcggtgattt tgaataagct taaacaaatc gtgatgttag gccgtcaatc tggtttcttt
    19381 ctgattctag cttgtcaaag gccggatgcg aaatatctag gagatgggat tcgcgatcag
    19441 tttaacttcc gtgtggcttt gggtcgtatg agtgagcttg ggtattccat gatgtttgga
    19501 gaagtcgata aaaatttttt tatgaaacgc atcaaaggac gaggttatgt agatacaggg
    19561 ggcagtgtga ttagtgagtt ttatacgcca cttgtaccaa aagggtatga tttcttaagg
    19621 gaaattagca acattgttgg attaaccgtt catacggaaa gagaaaataa tagtgtgtag
    19681 cctaggaata tcaataatcc tgggcttttt tattgaggtg atttatgaca tttttagatt
    19741 tattcgccgg gattggtggc tttcgcttag gtatggaaca agccggtcac cagtgtatcg
    19801 gtttttgtga aattgatgaa tttgctcgga ggagttataa agcaatccat gatacaagga
    19861 aggaggtgga aatgcatgac atcacaagtg tatcagacga atttgttcga tccctcggac
    19921 cagtggacat cctttgcggc ggctttccgt gccaagcttt ttcaattgcg ggaaagcggc
    19981 aaggattttc cgatactcga ggtactttat tctttgaaat cgctcggttc gccactcttc
    20041 tccaacctaa gtttctattc cttgagaacg tccggggatt gctcaatcac gaaggagggg
    20101 ctacgttcga gacgatcctc cgaacgttgg atggattggg gtatgatgtg gaatggcaag
    20161 tgcttaactc aaaggcctac gttccccaaa gccgtgagcg gatcttcctt atcggacatt
    20221 ctcgagatgc atgtaccgaa caagtatttc ctatcattgg atcgtcttca acatctgatc
    20281 aaaacatcag aaacttggta aatatcaatc cttccaatcg aggaatgggt ggccaagtct
    20341 atggttcaga tagcgtagca ccaaccttga ccggtgatga aggaattaaa attgccttac
    20401 cggtaaagga tgggatttct gtagcgggta tgttgtcggg aaattttgaa caaggcaata
    20461 ggatttatga ggtaaccgga acagccccca ctttgtcaac gaaacaaggg ggaacgaaga
    20521 ttatgattcg gaaaaatgaa acagaatatc aagaggtgaa gccaggggac agtgtgaacc
    20581 tggcttttcc taattccaca agtcgccgag gtcgtctggg aaaacaaagt gtgcatacct
    20641 tactaacagg agaccaacaa gcggtagtca cggatcagta ccagattcga aagttaacac
    20701 ctagggagtg ctggcggctt cagggctttc cagattgggc ttttgatcga gcttctcaag
    20761 tcaattcaga tagtcagttg tataaacagg ccggaaactc agtaacagtt ccggtcattt
    20821 ttgatattgc tagaagatta aagggggtaa aagcatatga tttatgacga gttgattgga
    20881 gaaatctact gggtgattgg caagattcag gcagatccga aattggaaga agaactgtac
    20941 cggctcaatt ttgatattcg caaaaacggg gtgaaagtcc aggtgattcg tatgtagtgg
    21001 atgaggaaac ggatgctcga atggagctca atcaagtcat cggtgaattg gaacgaattg
    21061 cggaccttgc caaagaacct gatatccgcc aatacttgtt tgagataaaa gcagagttgg
    21121 aaattgaggg tatcaccgcg gagtaatagg gaaataaccc actgataggc actgaccgcg
    21181 ggggatttgc gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac ggcggcagcc
    21241 ggccatgatg tctcaccccc cgtatctaac aggggggtac aaaaacacaa aaaacaagca
    21301 agtaaccata aaaaatgctt gttgtatcaa ggatattaag taatttgtat aggtgttaac
    21361 tcatttggaa aagttaacac cttgaatttg gaggtgaaaa cttggcgcaa cgaaatttag
    21421 attatcggtt attaaaagaa cgacgcaacg aatatggcgt ttcacaaaat aaattagcta
    21481 ctgcttgcgg acttagccgc ccatacctaa atcagataga aaacggtggc gtaatcgcct
    21541 ctacaaagac catgagaaaa atttttgatc aactggaaag tttcaatccc gatttacctt
    21601 taacgttact atttgactat gtaaggattc gctttcccac aacggatgtg cggaaaatta
    21661 ttcaagaaat tctccatttg aaattcgagt atatgcttca tgaagattac gccttttact
    21721 cctatcaaga acaatatgtc atgggcgata ttgtagtgat gttatctcat gaagaagata
    21781 aaggtgtcct tttggaatta aaaggtcgtg gttgccggca gtttgaaact tttttactcg
    21841 cccaaaagcg tagctggtac gacttttttg aagattgtct aaaagccggt ggcgtgatga
    21901 aacgcttgga tttagcaatc aatgatcgag tggggctact ggatattcct gatttaacga
    21961 aaaaatgtca gaaggaagaa tgtatctcct tgtttcgtac cttcaaaagt taccgttccg
    22021 gggaactgtt gaaagctgat gaaaaggatg gtatgggcaa taccttatat attggcagcc
    22081 tcaaaagcga ggtttatttt tgtttgtacg aaaaagatta tgaacaatat atcaagttag
    22141 ggattccatt ggaccagacc gaaacgaaaa atcgttttga gattcggttg aaaaatgacc
    22201 gtgcttatta tgcgattcaa gatttgttaa aaggtcgtag cattgaaagt accacgtttt
    22261 ccattatcaa tcggtatttg cgatttgcgg ataaagtaga tggaaaacgg cgaaccaatt
    22321 ggcccttgaa tgaacaatgg ggtcgtttta ttggacggaa tcggaaagag attcaattaa
    22381 cttctgaacc gaaaccgtat accattgaac gaacattaaa ttggctaggt agacaagtcg
    22441 cccctacgtg gaaaatggct aaggagttgg atcgattgaa tcaaacaacg tatatccagg
    22501 atatggtaca gaatgcgcga ctctcggacc ggcataaaaa gattttggag caacaaagta
    22561 tggcaatcga aaacttgatt gtatgaaaag aggaattttg atggaaattg taaaagatat
    22621 gttattggta cttggtggca gtatgcttgg tgttaccgtg atgtgcttga tgaatgcaag
    22681 tgcagcagcg gattgagcaa tggaaaagaa aggaaagtga caaaatgaat ttcggacaaa
    22741 atttatgtgc gactcgttcc ttagtgaaaa gctaaggcac taataaaaga acgaaaaagt
    22801 ttggttatta ggagaactga taatctgaca ggtggaatga tgaagtaacg ctctgaaacg
    22861 cctgccctga gcctccgaca agcattgtat gaggaaactc agaatgtttg aagctcggta
    22921 aagacggctg aaagataccg taatagttga tgaacatata tcttctatcg aaaactatcc
    22981 agaggtgggt agcgtatcca acaggtcggg ggtctataaa atatctatgg ttagaatgtt
    23041 ctataaggaa ctgacgaaac agcgaatgta cgggtctaaa tttgaacttt gtagaaatgc
    23101 aaaggtacga gaaatcgtag ctggtgtggc aaagtaagaa tataggttat gaaatgccat
    23161 ataacgttac aggcggtatc aagccagcag gcttatagct agtgacctaa ggatatatat
    23221 gtatagataa gattatcgga acgaggaaag gtattgggtt ctcatcaaga gaataaactg
    23281 acgaagaaca ataagcagtt gaactaatgc tgaaaagcag agggcgaacc taagaaattc
    23341 ttgtaatgag aatggaggaa tgccctcaag tcgtgtatat attagaaaag tattatgttg
    23401 tttcaataag gattacgagt aagataaaaa agctcactca atttcaagta aaggagtgaa
    23461 gccttatgcc taaaaataaa aatgaaacat tgtgtgtaga agatttaaga cacgctgaat
    23521 attacgaaat gcaaaacacc tttgatgatt tatatgccaa aagtaagaat ggagatattt
    23581 tcactcatct tatggatatt atcctatcga gagaaaatat attactggca tatcgaaata
    23641 tcaaagcaaa tgcaggcagt aaaacagcag gaacagacgg tacaataatt aaagatattg
    23701 gcaaattgcc agcagaaaca gttgtcaaaa aagtaagata cattgttgca ggcagtccac
    23761 atgggtatcg acctaagcca gtgaggcgaa aagaaatacc aaaaccaaat ggtaaaacca
    23821 gacctctagg tattccttgt atgtgggaca ggcttataca acaatgtata aaacaagtat
    23881 tagaaccaat ctgcgaagct aaatttagtg aaaacagcta tggttttaga cctaataggt
    23941 cagttgaaaa tgcaataaaa gcaacctata accgcctaca aatcagccag ctacattatg
    24001 tgattgaatt tgatattaag ggtttctttg acaacgtgaa ccattccaag ctgataaaac
    24061 agatatgggc tatgggaata cgggacaagc acttgatatt cattttaaaa aggatattaa
    24121 aagctccaat caaaatgact aacggtacaa taacatatcc agagaaagga actccgcaag
    24181 ggggaataat atccccacta ctagcaaaca ttgttcttaa tgagttagac cattgggtag
    24241 aaagccaatg gcaggaaaat cctgttacaa aaaactatgt ggttcatatc aataaaagcg
    24301 gtagcccatg taaaagcaac gcttacaaag aaatgaagaa aacaaagtta aaggaaatgt
    24361 atatggttcg ttatgcagat gattttagag ttttctgccg ctacaaggaa agttctgaaa
    24421 aagccaaaat agcaatcact caatggattg aacaacgatt aaaattggaa gtatcacaag
    24481 aaaaaacaag aattgtaaat gttagaaaaa gatactccga ctttcttggt ttcaagatta
    24541 aaatgatacc gagaagaaag aagttagttg ttaaatcaca tataagtgat aaacaattca
    24601 aaaaccaaaa agacaagctg gtatcacagg caaagaaaat tgcttctccc tcaaaaggga
    24661 aaacggaatt agaagaaaca agactttata attcaatggt attaggtatg caaaattatt
    24721 atcagattgc aacgaatgta aatctggatt gtatgaaact gaatagagct gtaatggcta
    24781 tcttcactaa cagattaggt acaaacaaaa ggggcagatt gagaaaaaca ggcagacaac
    24841 ttacgacaaa ggaggcaaca agatatggta aatctaaaat gcttcggtat gttgctggcg
    24901 agggcgaacc tattttccct gttggatata tccaacacag aaaaccaatg gcaaaggtgt
    24961 atttagcgaa ttgctacact ctagagggaa gaaaatttat ccacaccaat ctaagtgttg
    25021 ataaattact gatgtaccag ttaatgaaat tatctttgga aaatcgcaca atagaatacg
    25081 cagacaatcg aatttcgcta ttttctgctc aacatggaaa atgtgctata actctggaag
    25141 aatttcaaca agctaatgaa atacattgtc atcatattgt accaactgga gctggtggaa
    25201 atgacgatta taaaaatctt attttggtaa aagaggcggt acaccgtcta atacacgcta
    25261 aaacagaaga aacaatacaa aaatatatag ctctattgaa gctaaataaa gtccagctca
    25321 taagactaaa taaatataga gtattagctg gcaatcaaga attaaacttg atataggtct
    25381 actacataaa tacttttcaa tataaacacg atggaacgcg gagtgcggtg aaagtcgcac
    25441 gctccgtgtg aatgggggga aaagtttgag atatttataa tatcaggaac ttacctatcc
    25501 atatatcaat ggtttttaac caatgctcaa tctttagtct tactagcgat tgtggtcatt
    25561 ggtttgtttt taggatttaa acgggagttt tccaaactga ttggcttctt ggtgattgct
    25621 ttgattgcgg taggtctggt ctttaatgct tctggtgtga aggatgtcct actcaatttg
    25681 tttaaccgaa ttgtcggtgc ctgaggggga atcacaatgg aacagatgcg tgtttatatt
    25741 gcgaaccttg gaaaatacaa tgaaggtgag ttggtggggg catggtttac cccacctgtc
    25801 gattttgatg aagtgaaaga acagattgga ttaaatgacg aatatgaaga gtacgcgatt
    25861 catgattatg aactgccttt tgagattgat gagtatacgc caattgaaga gattaatcgg
    25921 ttgtgtaact tagcggagga actagaagga acgccaattg gtgaagctgc ttctgagatt
    25981 caacacgctt tcttcaattc tttcgaggaa atggtagagc atgtggatga tattgtctat
    26041 tatccagatt gcgatgatat gagtgatgtg gcgcattact tgattgtaga agctggtgat
    26101 tttggcgaag ttccagaaca attgaagccg tatattgatt ttgatgcttg cggtcgagat
    26161 ttggagattg gaggaaatta tttggttact agtcgtggag tttttgagta tccagaatga
    26221 atcaaacact atatatagtg tttgattagt atatgtaagc aaaaaaacac aatatataga
    26281 gtgcaaactg aagggtaatg tgatataata tttgccgaag aaatattttt gaaatgaggt
    26341 aatattatgg caaatattca aacaagtttt attgatttcc ataattcaat tagattggac
    26401 gtagaagaca atacgctact taaggactat aaagaccaag taattgatgg attaaaagat
    26461 tacttacctg atgatgtgaa gtttgaaact tttttacagg gaagctattc tgtttatacc
    26521 ggaataaaaa gctgtgatga aaaaattgat tttgacatcg atattgcagt tgctttcgaa
    26581 attgatcata ctgtttatga agatccaagg gagcctaaac tgtgggtaaa agaggcgcta
    26641 gttgaaattt ttcctaatgc tcaagttaat ttaaaagtac cttgtgtaac tgctacattt
    26701 actggaaaaa agacaaaaaa aaatgtgcac gttgatgttg cggtttatgc taaagaagat
    26761 gaaaattatt tcttagcaaa agcaaaagaa ttcagtgctc ccgaaaaccg atgctgggag
    26821 gaagcggatc ccaaagtatt aaaagagaaa attaatagtc atgtagcaga ttcagatgat
    26881 cgaaaacaat ttcgaagatg tattcgctat ttaaaaagat ggaaagataa caattttaac
    26941 caagaatata aaccaacagg aattggactt acaattaatg tgatggatac gttcctagtt
    27001 aataagtcca ctgatttttt aactagaaaa gttcagtata atgatatgga atgtatgaag
    27061 cagattgttt cttcgctgaa agattcattt gtgtatgagt acagtgaaac agatggttgg
    27121 cattatcgtc tacatgcata tcttccagtg aagcctaaca gtgacaccta ttcaaaaatg
    27181 acagtgaatc agatgagtga ttttaaaaac aaactttcta aattatatga tgatttgatt
    27241 ttcgctatcg atactgaaga tgagtatgaa gctacaaaac gattaaataa tcaatttggg
    27301 gaggattttt taattatttc agaagaagaa gttacagaaa aaaacttaag aaatgcattt
    27361 gttacagact atccgagtgc ttaggttggt gttaaagatt ggattatact gatattttaa
    27421 aagaaaagct aacagatgtt gtgcgtttat tcgatggtaa ttactatggg gatttttcat
    27481 ttgctacaat tggaaagttg cgtttaaaaa ttgaaatacc aaaacatttt ccattggtac
    27541 ttcctaggat ttacattcat aattttgatc atttaggact ttacctacca catgtagaaa
    27601 aagatgggtt ggtatgtttt tcttcagata taaattattt agtagatgaa gaaaattcag
    27661 aattggtttt attgcagtgt gttaataaaa ctataaaaac aattgaggaa ggtttgaaag
    27721 ggttaaataa aaatgatttt cgaaaagagt ttgttgcttt ttgggacaaa caagagaatc
    27781 aaatttcgtt attctacctt tgtgaaccag cgttgtttat caagtcagtt gatacaataa
    27841 tgtacggtaa gtctttggtt gctttggata aagagaacga attacaatta aatgcagcga
    27901 gtagattgtt taatggagag aaagaagata ctcatgaatt agatgcatac tatgttccaa
    27961 ttagaaacaa gaataatatt atgcccccga agaataataa agcatggtca aagaaggata
    28021 ttttacaaat agtaaaaaaa aatgtaacag gtgcaaataa aagatttttt ttaaaacttg
    28081 aaaaaagaaa gaatagacat aatgttttac ttattctaga tatcccaatt gaagaaaagt
    28141 caaataatgt cataattgga tttcttttta caaaacaatc tggatctata aaagattata
    28201 agggtacgcc taggactgta ttagttcagc gtcatgatga attatatttg gtgaatagaa
    28261 cgagcggaga aaactatgga ttgggtgcta atatagctat tgttggggta gggtcagttg
    28321 gcagtaaaat tgctgaaaat cttggtccat taggtgtgag aaagatgaca ctcatagaca
    28381 atgattgttt tcaagctgaa aatttgtatc gaaattcact tggtgcagat agtttaaaaa
    28441 atgcccgatc tcagaataat ttttttaaag ttgatttaat gcaggaggag ctcgaaaaaa
    28501 aattcccata cctttgtgtt gaagtggagt caatgaacgt tttagactta attagtatgc
    28561 aacctggttt tttcaaggaa tatgactatg tttttgtttg cataggagat acaatggcaa
    28621 gcctggcttt aaataattat ttttccaaaa gtagtcaaaa agttttctat tcttggctgg
    28681 agccatatgg cattggggga cacactctat ttataaatta ttctctaaat caaaaaggtt
    28741 gtttcaattg tctttataca gattcaaact ctaaaaatag gtgttcaaat agagcttctc
    28801 tggtaaaaga aggacaaatt tttgaaagga ctttagcgag ttgccgctca aaatttatac
    28861 catataatgc tattgcctcg acggaatgtg caattaatgc aataaaactt ttccataaag
    28921 tcaatcaaaa taatatgacc aatagtactc ttttttcatg gataggagat gggagtactt
    28981 ttgagaattt aggatatcaa tatagcgaca gaaatacttt tcttaatgga gtcaataagt
    29041 ttgaagaaag attcttttct aaatattgca aagtatgtgg ggagttagat gattaaattt
    29101 tcgtgtgacc gtattgtcat tgaagtaagg gatgaagttc ttaagatatt tgaacaatat
    29161 cggcagggaa aggatgactt gcctgaagct ggaggagttc ttcatggaaa aagacttaag
    29221 aatgggagca taataattac cgatataaca acacctcaaa taggagatat acaaaaaaga
    29281 ttactcttta aaaaaaaccg agaaattcat caacagcttt ctaatgagaa atggatagaa
    29341 tcaaataagt tttctattgc attgggagaa tggcatactc atcctgaagc tgtaccaact
    29401 ccatcaaatg ttgataagaa aagctggaga ctgaacgtga gcaagcaaca tgatgataga
    29461 gtttatgttt ttattatcgt tggattttct gatttgagaa tgtggttact gtcaaaaaaa
    29521 tcagatatta ggcaggctat aaaactataa aggagtgatt aggtgaaatc acaaaagaat
    29581 atttggtggt gggggttttt agtaggaaag gtaatgatac cagcccttct cttaatttct
    29641 ctaccgatga ttactttttt ctttaccaat aaaaaaatga gtaaagaagc cgtgattttt
    29701 ttctttggag accaaaaagc tacctttatt gaaacgttag taacatctct tcagcttaat
    29761 ttgaattaca ttttttcaat tattattgtt atatcgttat tgaatttttt taaaaaaaga
    29821 aattcaggaa aggtgtttaa ctctaatgga aatgtgtact acaactattt ttattttgta
    29881 ttttgggtag cagcaacttt acttggttat gataaaattc agattgcagg aattcctata
    29941 cacatgcaat ataagttggt tttgagtgga attttttctg aggtgttgcc agatatatac
    30001 gatgatcatt acgacagtga tggaacatgt aaagtaagca ttgaaaaaga aaatttcgat
    30061 gacatcgatg gatatgactc tgtgaactta ctgattattg atacttacga tataaaaatg
    30121 agtgaattat ctatggaaaa tcaaacatat cctacgataa ttgttagagg gaattcaata
    30181 gatggagttc gtaaagttaa tagatcttta atcttggaaa taaaaaaaac aatggatgaa
    30241 attcaaaaaa gtgactttaa aaaagtattt gttgcttcaa catctaatcc aaaaaatagt
    30301 atcaatatta ttaattcatc gtttagattt ttcggtcgat cacgtaggtt taaactttat
    30361 gttcttcaaa aggattatgc aagcaatgga aagtatagta agaaatatag aatttttatt
    30421 taagaaggaa tttttccttc tttttatttt ggaggaaact atgaaaaaaa taaaaagcta
    30481 caccagtatt tgggcagttg aaaaagtcat ttatgcgatt aatgactttc agctgccttt
    30541 tcctgtgacc tttaaccaga tggcttggtt tgttttatcc ttgatgtttg tcattgtatt
    30601 tgcccatgtg cctccgctat ccatgataga gggggctttt ttaaaatact tgggcattcc
    30661 gatagcggtc acttggttca tgtcgcaaaa gacctttgac ggtaagaaac cattgggatt
    30721 tttacgatcc tttatcagct atcacttacg gtttaaggtc acttttgcag gaaagaaagt
    30781 taaagaacag aagaaacgat gggacgaacc aatcaccttg gtaaggagtg tgaattatgt
    30841 acccgattaa gtatattgaa aataatcttg tctttaacca agaaggcgag tgttttgctt
    30901 actatgaatt agtgccttac aattactcct ttttgtcacc tgaacagaag tatcaagtac
    30961 atgataactt tcgccaatta attgcgcaaa atcgtgaagg taagatccat gccttacaaa
    31021 ttgccacaga aagtagtatt cgtgccacac aagaacgttc gaaaaaggaa attaccggta
    31081 gactcaaaga agtagcaaag caacgaattg atttacagac agatgcatta gtatccatga
    31141 ttggcgatag tcagattgat tatcgcttct ttattggctt taaactgatt tcgacagatg
    31201 aagaggtcaa tctaaagaat ctcaggaaat ctttcttttc tggacttcaa gaatttgtct
    31261 atggagtgaa tcatcatttg atgggtgatt ttgtttcctt atccaatgaa gaaattcgtc
    31321 gctataccaa gcttgaaaaa ctaatggaaa gtaaattggc tcgaagattt aaagtcaaac
    31381 gagtaacgcc gagtgacttg acgtatttaa ttgaacatat ctatggtgaa aaaggcacac
    31441 cgtttgaaga atatgaattc cagctaccaa agaaaaagct gaaatcagaa acgctggtga
    31501 aacgttacga cttattacgt cccactcgtt gtttgattga agaaaagcct cggtacttac
    31561 ggatggaaca tgaaaatcat gagtcttatg tagcgtactt aacaattaat accattgtag
    31621 gggaaatgga gtttccttcc tcggaacttt tctattacca gcaacaacaa tttacgtttc
    31681 cgattgatac ttcaatgaat gtggaaattg tgacgaataa aaaggcccta gctaccgttc
    31741 gtaataagaa aaaggaatta aaagatttag acaaccatgc ctatcaatct gataatgaaa
    31801 cgaactccaa tgtattagat gctttggatt ccgtggatga attggaaacc accttggatc
    31861 agtcgaaaga atccatgtat aaactaagct atgtggttcg agtgagtgct aaatcagtgg
    31921 atgaattaaa acgccgctgt gatgaagtgc tcgattttta tgacgatacc aatgtaaaac
    31981 tagtccggcc ctttggcgat atgatgggtc tacacaaaga attcttaccc tcaagtaagc
    32041 gatacatgaa tgactatatt caatatgtta cgtctgactt tttggcggga cttggttttg
    32101 gggctactca aatgctagga gaattagaag gcatttactt tggctataac gtagatacgg
    32161 ggcgcaatgt ttacctgaaa ccggcattgg catcacaagg ggtcaaaggc tcagtaacca
    32221 atgctttggc atctgctttc cttggttcgt taggtggggg gaaatctttt agcaacaatc
    32281 tcttagtcta ttatgccgtc ctttttggtg ggcaagctgt gattgtcgat ccaaaaggag
    32341 aaagaggcgg ttggaaagaa acgttgccag aaattgcgga tgaaattaat attcttaact
    32401 tgaccagtaa gccagaaaat caaggcttac ttgatcccta tgtgattatg aagaaaaaga
    32461 aagactcaga aagtttggcg attgatatcc tgaccttttt aacagggatt tccagtcggg
    32521 atggggagaa gttccctgtg ttacgcaaag ccattcgaac ggttacccaa agtgatcagc
    32581 gaggattatt acaagtaatc gttgaattgc gtaaagagga ctcaccagtc gcggaaaata
    32641 tcgcggatca catcgagtcc tttaccgatt atgattttgc ccatttgctt ttctcagatg
    32701 gttcgattac ccagtcgatt agtttagata agcagttaaa tatcattcaa gtagcggatt
    32761 tggttttacc agatgcagaa accacttttg aagaatatac caccatggaa ttgctgagtg
    32821 tttccatgct aattgttatt tccacttttg ccttggactt tatccatagc aaccgagaaa
    32881 ttttcaagat tgtggacctg gatgaggctt ggagttttct tcaagtagct caaggaaaaa
    32941 cgttatcgaa taaattagtg cgtgctggtc gggccatgaa tgccggtgtt tacttcgtca
    33001 cgcaaaacag tgatgacttg ttggatgaac gtttgaagaa taatattggg ctaaagtttg
    33061 catttcgcag taccgatatt catgaaatca agaaaacctt ggaattcttt ggattagata
    33121 aagaagatga aagtaatcaa aaatgcatgc gagatttgga aaatgggcaa tgcttgatgc
    33181 aggatttgta tggtcgagta ggtgtggtcc aagttcatcc agtctttgaa gaacttttcc
    33241 atgcttttga tacccgtccg ccagttcaag agagaatgga gtgaggagat gaaaaagaaa
    33301 ctttttcgta tctttggaat tgttttaatc agtttggtca ctctgataat acttttgagc
    33361 ttagttggaa cggtagctga ggcaacaggt ctggttgatg atacagttgg agccggaaat
    33421 ctttattccc aatattcact tggtaactat cagttggatt tctttgtgga tagttcgtgg
    33481 gattggctgc cttggaattg gggtgatggc ctagggaaga gcgtaatgta tggactttat
    33541 gccattacta attttatttg gacagttagt ctctacttat caaatgccac gggctacgtg
    33601 gttcaagaag cctataagct ggatttcatt tcagatacgg ctgaaagtat tgggaagaat
    33661 atccaaacct tagccggcat taccgaaaat ggactccaga cctcgggctt ttactttggg
    33721 tttctattat tgatgatttt agctctagga gtttatgtgg cgtatacagg attattaaaa
    33781 cgagaaacta ccaaagcagt ccgggctgtc attaattttg tgatgatttt tctactttcg
    33841 ggttcgttca ttgcgtatgc gcccacctat atcacaaaaa tcaatgattt tagctcagat
    33901 gtcagcgaag cggctcttag ccttggaacc gagattgtcg tgccgaattc agaaagccaa
    33961 ggaaaggaca gtgtggattt aatccgagat agcttgtttt ccattcaagt ccaacaaccg
    34021 tggttactct tgcaatttga tgattccaat atagaagaag ttggcgaaga tcgtgttaac
    34081 aaaatcctgt ctgtaagtcc agatgaaaat aaaggcaagg atcgggaaga agcggtcaaa
    34141 gcggagattg aagataatga caatgcgaat ctcagtatta ccaagaccat gaaccgctta
    34201 gggaccgtgg tctttttggt gttgttcaat attggcatat ccttctttgt ctttctccta
    34261 acagggatta tgttgttctc gcaaattctc tttattatct ttgcgatgtt tttgccgatt
    34321 agtttcttat tgtccatgtt gccgacttat gagagtttgg gcaagaaggc gattattcgc
    34381 ttgttcaata cgattatgat gcgagcagga gttactttgg tgattacgac tgcatttagc
    34441 atctccacga tgtttttcaa tatttcggca acctatccat ttttcatggt tgcttttctg
    34501 caaattgtga cctttgccgg tatctatttc aaattgggcg acattatgaa tatgttcaat
    34561 ctccagagta atgatagcca atcaatggga agacgggtca tgcgaaaacc gcaaatgtta
    34621 atgaatcgaa cattgcggca actcaatcga aatgtgggac ggactttggc ttttggtggt
    34681 ggtgcagcag ttggaaataa gttggcaaag gaacaatcaa aatccaaatt taagccatcc
    34741 ggttcttctc tacgaaagaa ttcacgatta ccgaacgatc acgagatacc atctgacttg
    34801 aataaggaaa agccaattcc aaataataag aaacaatccc gaatgaattt ggccgggaga
    34861 aaagtcggca aggttctgga tacccaagct ttggtgaaag ataagaccaa acaagtaaag
    34921 gatggagtac ggaatacgcc aaccaacttg aaatacaact tccataaagg aattgaaaaa
    34981 acgaaaaaag caccggaaga gtttaagcga ggactggtcc aagaaaaagc aaatcgagga
    35041 gagttgcgag aaaaacaaca acaacgtaga gacgaaaaaa tagctgaaaa gcgcaaagta
    35101 ttgaacgaaa tagggaatcc tcatgggaaa agaagggaaa atgttccgat aaaagcaaag
    35161 gtccaatctc aaaaggatag agcaccaaag cggattgtga gagcaaattc gaatcccgaa
    35221 atcaaacgaa aattcgctag ccaagaaatc atttcaaaag gaataaacca atcctcaagt
    35281 aagaaaaatt cttttcaaca agtgaaacgg cggtctactc tgtccaaacg aaccaatcga
    35341 aaagtacaaa aaagtttgaa ccctcatccc aaaccaaggt caggtgagaa aaaatgaacg
    35401 taaagaaatt aagtgccatt tcgttaactt tcctaacaat ctcttttatg ggtatcttga
    35461 tgtgtgtggg cttattgttt ggtgaagaca gtgaaggaag tagtagttca ggtgctcctc
    35521 ctggggggag tagtgtctca gaagaggttt tgaaacaccg tgcaatggtg gaaaaatatg
    35581 ccaaagaatc ggatataaca gagtatgttc cgattctttt agcgattatt caagtggaat
    35641 ctggtggcac aatggaagat gtgatgcaaa gttctgagtc agccggttta ccaccgaatt
    35701 cattaagtac cgaggcatcc atcaaacaag gatgtctcta ttttgcgtct ttggttaagc
    35761 gttccaaaga attaggttgt gatcaagata gtatcattca ggcctataac tttggcggtg
    35821 gttatttgga ctatgtggca aaaaacggga agaagcatag ttttgcttta gctgaatctt
    35881 tttcaaagga aaaatcttgc ggtcaaaaaa tcgattaccc taatcccatc gcgaataagg
    35941 aaaatggtgg atggcgctat aactatggca atatgtttta ttgcttattg gtgaagcaat
    36001 acctaactac aacacagttt gacgataaaa cagtccaagg gatttttgac gaggcgttta
    36061 agtatgaagg aacggcttat gtatttggtg ggtcaagacc cgaaacagga tttgattgta
    36121 gtggcttgac tcaatggtgt tatgccaaag ttggtctgaa acttcctcgg gtggctcaag
    36181 atcagtatga tgccatgacc catattgacc taaaagatgc caaaccaggg gatttggttt
    36241 tctttcattc gacttatgat gccggtactt atgtgaccca tgtcggtatt tatgcagggg
    36301 agaatcgcat gtttcatgca ggagatccag taggctggac gaatctaaca gaaagctatt
    36361 ggcagcagca tcttatcggt gctggacggt acaaataaga gaaaagagga aaaagaatga
    36421 agataaaaat tgaacggaat caaaaggaaa agagcctaaa aaagaaaaaa gaacgagtcg
    36481 tatccgtggg taagcatcga aaaatggtgc ttgccctttg gcttttatta tcttgtagtc
    36541 ttacttttgg cgtttataaa aatttcaccg ccattgatca acatacaact catgaaaaag
    36601 tggttattaa agagaaagta gtgaatactt ctggtattga aaactttacg aaggattttt
    36661 cgaaagaata tttttcgtgg aaaaataaca aagaagcaat tgaaaagaga atgaacaacc
    36721 tcgaacagta ccttacagaa gaaggtttcg ctttaagtca agatatggtt cgagcagata
    36781 ttcccactaa ttccgaagtt cagtcagtaa aagtcttaga tgttgaaaag agctcggagg
    36841 aatttgtcgt ttcattttta gtggagcaaa agattacaga gggaaaaaag gcacaatcga
    36901 tttcttctgc ataccgagtg acgatctttg aagatgaaaa caggaattat atagtaacga
    36961 gtttgccaac tatgattgca aaacctgata gggcaaaata caagacaaaa caagtagaaa
    37021 atgattcgaa aattgatgcc aaaactacag aagaaatcgc tgagtttttg gaaacgttct
    37081 ttaaattata tccatcagct tctgaaaaag aattggagta ttatgttgaa aatgatgcga
    37141 tgcgaccaat caatattgac ttaaagtttt cgaagttaga ggatccagtt tacctacaaa
    37201 acggaaaaaa aattctagtg agtacaaaga taaaatacct tgataatgta tcaaaaacca
    37261 ctagctactt tcagtacaaa ttaatattac aaaaaaatgg aaactggaaa atcattgatg
    37321 ttaaataata atgactcaat ctaaatttat gagaagttat aagtagttta acttatttca
    37381 ggcagaagtc gcccatcctc tataggtggg tgatgaatgc cgttcggtaa ctcgaaaacc
    37441 gacaattaag acatagtgcc tttttatttt aatccatgtc ataatcagtc tatataagaa
    37501 atggttgata taccatggaa ttagatacaa ataatcattc attatttctt ctttgttacc
    37561 accttatttt agtagtgaaa cattgtaaac atctcattga tgatgtaatg ttagcttatg
    37621 caaaaaacta tctttgaaag aatatcagaa tcacatcaca ttaccttagt tgagtgaaat
    37681 cgtgataaag actactttca tattatgctc aaagcacaac ctaaaaccga attaacaaaa
    37741 ttcagcaatg cctaagctca tgtctgataa aatgagactt tctaagggtc aaaaatttcc
    37801 tttgaatacg atcattagct tagataaaag taaaaacctt agaactacgg ggatagcttg
    37861 gttaataaga gatatcgctg ttaataaaga cataagcttg caagtatgct ctattcccaa
    37921 gaatctccca cttcaagcac taaggatcgt taaattcagc taagtggcaa gtagttcact
    37981 agacgataat tatctccata tttcaatctc agcttttcca tttttcatag caaattccgt
    38041 tatttttata gcatctaaaa gattcagctt ttccactgat ctttcaccac ttttcagctt
    38101 ttttattgtg cttaaattga taccagtacc ttttgatatt gaattggaag taaaatcatt
    38161 aagggttttt tttattaaat tcatatccgc aattccagcg ttcataagaa tgaagattct
    38221 cctctcatag actactttga aaatggtact acagtggctg tttatatatt tcagtaggtt
    38281 actgtagtgg tcattttatt ataattagaa aaggaaccta aatcaaaaat gctaaagaaa
    38341 attttcatag tattaaacct atcatgacta aagccacgga attcttgata aaagagtata
    38401 cttgcaagcc tatgtcttta ctaacagcga tatctcttat taaccaatct atccccgttg
    38461 ttcctaaggt tcttgatgtt ttctaaggca atgcttgtat acatagccct tgatcaaaat
    38521 attgatacta acgttgacgt ttcgatcatg atgtgtacgg caaataggac aagtccattc
    38581 tcgaattgtg aatgacttct taccatctgt gtgtccacat tctgaaccaa tttgactaga
    38641 cggaaatcat ttatctatct tgatgatttt tttgacagaa gtatcgaaaa tctttacata
    38701 atctgggtgt tgcattctta tgcagatgag agcgttagct ccgtattcat aaccagttgc
    38761 ctctaaaacg tagtccatcg gtggatacct agcttgcggt tgccagtggc tttcgcagtc
    38821 ttcttgtaat caaatcgagc atctaattga gtactagtat tttgaagata atacttgagt
    38881 ttttcggttt tcagaagttc acttcatctt ccatcatcca tggaaatccc actcacccat
    38941 acgcaggcct ccaaaaattc ttcaacaatg gtttttcaga catttttctg aatacctcct
    39001 tgttttttga tacgccagat agtcaggaaa aaggcaacaa aacacaaaac aaagctgcct
    39061 aaaaaggtaa tcttcattcc atataaaaag acatccggcc gatctgcgat atagttagtt
    39121 actcgttggc ccatcttagc agacatcgct tgatataaga ccgtcgtaga aagcgaaatc
    39181 ccgatgacca tccccaaatt tcgggcaaag gaattcatgg agcctgccag tcccaaattt
    39241 tcgatagtga cagaactcat aaccatcgta ttatttgggg actggaacaa ggcattgccg
    39301 gcacccatga tggcagtagc gacaatgtac agccacaaag gagagtccac tgacaaaaac
    39361 atatacatga cttgggttac cgacaataaa cccaagccta ctaatacaaa aatctccggg
    39421 ccaacccggt cggtcaggta tccgcttaaa ggcgccccga cgaccatcat caagggaaag
    39481 accatcatca acagtccggc atagctagga ctcaacccgc gagcattttg cagataaaaa
    39541 ggaatcacta cattaacaaa gaagttggaa gaaaaaatca acaaggctgt aatcaagctc
    39601 atggtaaaaa tcttgttctt aaaaataccg aaagaaatca atggagaggc tgctttcttt
    39661 tcaactttta aaaagctgac aaaggcaatc actgaaatta cgaacagtac aagggggact
    39721 ggatggaaaa agccactttc ttgccccaaa aagattgttc caaaaaggct gataatcgta
    39781 gccgcaatgg cagcaaagcc ccctccatcg attgtatggc cgctcatggt gatatctttt
    39841 ggtaagaatt tttcagcaat caacatcgtt acgattccca ccggtacatt gatccaaaaa
    39901 atgtacgccc aagaaaattg ggacaaaatc aagccgccaa gacctggtcc agcaatcgag
    39961 cctaatgaaa caagcgcacc gattgctccc aatgcccgac cacgttcatg tatgggaaag
    40021 acttccgtga tgattccggc atttgtagcc atagtcatac tagcaccaat ggcttgtacc
    40081 acccgtgcga acaattaaat acctagcgaa tgattgaacc cacacaacag cgagcccaag
    40141 acaaagatca gcgtaccaat ccgaaatact ttgattttcc cacagctatc gccaatttta
    40201 ccaaacagca acaaacaaga acagacaatc atcagataaa cggacactac ccattccgct
    40261 tgattcattg gtatgcccat atcatctgag atggtaggta gtgcaatatt gacaatactg
    40321 ccatccaatg tggacatgaa ggtaaacata gatatagcaa tcaaaatcca ccagcgattt
    40381 ttttggactt ttgcatcttc ttgataacta tgcataatag gatttccttt ctaatgatcg
    40441 tgtagctaag aacaatataa cctatgtcta ctgcccaaca acttctcgcg actctagcca
    40501 taggaagctt gagtaatttc gatttgttat tctcatgtaa ccatgtaagt ttgagatgct
    40561 ttatttgaac ttactctata gtaactagtg ataattcgct ttactttctt ctagcaattt
    40621 ctaaaagtcg acttcattgt tcttcagatt aatagtgtga gctatctcgt gagacgtaag
    40681 aaaatttcca ttctactttt tcccaatttt ttcatattta tggtgttgca cctatttgtt
    40741 ttccaatttg cttttcaaat acattgctat gatattcaat tccataggat tatactaaat
    40801 gtcaatagta tgaataatat attatagatg tttatatatc actgttaatt attatccaca
    40861 ataattaatg attacctcga tatttgtcaa tcaaaggttg aaacaattta ttttcagcat
    40921 cttttctaac ttgagctgca ctcttaatag aatcatacga tcctaaatag tacctctttt
    40981 ttttaaagtt atatacgcaa tccatttctc tttagaacga taaactccct taacaccact
    41041 tgtattattt ttcggtaatt ttgacgttag acactctatt actgtccctt caacacatgt
    41101 ccttgtttct tgccttttta aacatccaca actttgagta tgtccaaaaa ataagttact
    41161 ataacttatc tcacacatac tgccacactc acattgacaa acccattttg ttttccatga
    41221 acagttattg tccaaaggat actttgcagt aagtaatccg aattttttat tttcaatgtg
    41281 atgctttttt gatttcaaac atccacagct ttttacatca ccttttagaa gtcgctttgc
    41341 cgatactaac ttttttctcc cacacagaca ttgacattcc caaacaacag ttccttcact
    41401 tcgcagttta gttggcttga ttgcaactaa cttaccaaaa gttttatttg tcaaattttt
    41461 atatttatgc ctatttttat aatttccaca tccacaactt ttggttctac ctgatagtaa
    41521 gtttgatcga tatacaatcg tttcattccc acagtcacat aggtaacgat attttttata
    41581 tccattatga gtaatctcct caacaacagt aaggtgacca tatctttcct tcactgcacc
    41641 taccctccca agaaagaagt accatataat ttcaattaac ttttaataaa tcagaaaaaa
    41701 atattttcaa tttttcttag tctgatttta tgactaattt ataatacaag actgaacgat
    41761 acgtacacga aatcctcaag atagataaat tgttttcatc tagtaaaacc tgctcaaaat
    41821 atgagcataa agacggcaat aatacactcg atattcgata atggacttgt tttatttgtc
    41881 aaactcatcg taatcgagat atcaattcca gtctcaatat cctgatcaaa ggtctaagaa
    41941 tccaaccatt ttcttgttac tttgtcagat tgattacctt ttttagtctc cgaataataa
    42001 ggttttcgga tcaactaaca tgctctgaga aagactacta tctttgcaaa tttccaaatg
    42061 aaggtgtggg ccagtactat tcccagtact tcctacatag ccaattattt gtccttgcaa
    42121 aacattatct ccagttttta ctaggtattg ttgctggtga gcatataacg cggttgatcc
    42181 atcttcgtgt tgtatcacca cgtaatttcc ccaagagtag tgaaactctg ctttaacaac
    42241 cttaccagct ttaactgcat gaatcggctc accctgtgta gctgccatat caagtcctcg
    42301 gtgaaattca tttccacgat ttccaaacaa actggttatt gaataattat gaataggcac
    42361 ttggtatcca gtcttactca tttgtggacc tttaacttct ttatcatagt tggttaaatc
    42421 ataggtttta attaattcat ttagcttcaa atagtattgt gtatctgttg cataccgtcc
    42481 tgtaaggaat ttagttgctt ctcgatatgt tttactatta gcttttaatg ctcctgcata
    42541 aaaatcttta tttccactaa ttcccttagt taataatttg gcataatctt caaaactttc
    42601 ctcatagttt tcatacactc taaattttga ttgggtcgta taaagcgttc cattacccaa
    42661 atcttcgttt gttaccataa tgacaaagtt tccattataa gttcctttta tcccaaataa
    42721 attgtaattc ggtgcctgag ctaactggct ttgaccactg gcagattcta agatcgcttg
    42781 tgcaatcata acagaagcat ataaatcata tttctgacca atttttctcg cagactcacc
    42841 aattttcata ataaaatttt ctacagattc atttttgtca aaatgaattt cttcattacc
    42901 agatagtatt ggttgtgatt ggaaagattc tgttgattct atttgctgtg gtgtcgaaaa
    42961 attttttgtt tgtccttccg tttttggcgc ttcctttttt gaagactcta tatggtttgg
    43021 tcttgtcact ttcgaagaag ttatttcact tgtactactt gaagttgttg aagattcggc
    43081 acttgaa
//
LOCUS       DACCKU010000015        41837 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.015, whole genome shotgun sequence.
ACCESSION   DACCKU010000015 DACCKU010000000
VERSION     DACCKU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.015"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(644..1624)
                     /locus_tag="IVN13_000746"
     CDS             complement(644..1624)
                     /locus_tag="IVN13_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP7011257.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(1637..2515)
                     /locus_tag="IVN13_000747"
     CDS             complement(1637..2515)
                     /locus_tag="IVN13_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7011258.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(2531..3331)
                     /locus_tag="IVN13_000748"
     CDS             complement(2531..3331)
                     /locus_tag="IVN13_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAP7011259.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            4723..5049
                     /locus_tag="IVN13_000749"
     CDS             4723..5049
                     /locus_tag="IVN13_000749"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7011260.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            5057..5965
                     /locus_tag="IVN13_000750"
     CDS             5057..5965
                     /locus_tag="IVN13_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7011261.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            6594..6845
                     /locus_tag="IVN13_000751"
     CDS             6594..6845
                     /locus_tag="IVN13_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAP7011262.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            7387..7545
                     /locus_tag="IVN13_000752"
     CDS             7387..7545
                     /locus_tag="IVN13_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011263.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            7617..8843
                     /locus_tag="IVN13_000753"
     CDS             7617..8843
                     /locus_tag="IVN13_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7011264.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="IVN13_000754"
     CDS             complement(8932..9324)
                     /gene="rpsI"
                     /locus_tag="IVN13_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP7011265.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(9340..9783)
                     /gene="rplM"
                     /locus_tag="IVN13_000755"
     CDS             complement(9340..9783)
                     /gene="rplM"
                     /locus_tag="IVN13_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP7011266.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(10303..10770)
                     /locus_tag="IVN13_000756"
     CDS             complement(10303..10770)
                     /locus_tag="IVN13_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP7011267.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10890..12428)
                     /locus_tag="IVN13_000757"
     CDS             complement(10890..12428)
                     /locus_tag="IVN13_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP7011268.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            12508..13194
                     /locus_tag="IVN13_000758"
     CDS             12508..13194
                     /locus_tag="IVN13_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP7011269.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(13395..13922)
                     /gene="msrA"
                     /locus_tag="IVN13_000759"
     CDS             complement(13395..13922)
                     /gene="msrA"
                     /locus_tag="IVN13_000759"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7011270.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14006..14683)
                     /gene="pgmB"
                     /locus_tag="IVN13_000760"
     CDS             complement(14006..14683)
                     /gene="pgmB"
                     /locus_tag="IVN13_000760"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7011271.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(14680..15810)
                     /locus_tag="IVN13_000761"
     CDS             complement(14680..15810)
                     /locus_tag="IVN13_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7011272.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(15810..17993)
                     /locus_tag="IVN13_000762"
     CDS             complement(15810..17993)
                     /locus_tag="IVN13_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7011273.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17990..19015)
                     /locus_tag="IVN13_000763"
     CDS             complement(17990..19015)
                     /locus_tag="IVN13_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7011274.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(19060..19887)
                     /locus_tag="IVN13_000764"
     CDS             complement(19060..19887)
                     /locus_tag="IVN13_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP7011275.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19884..20951)
                     /locus_tag="IVN13_000765"
     CDS             complement(19884..20951)
                     /locus_tag="IVN13_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP7011276.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20977..21813)
                     /locus_tag="IVN13_000766"
     CDS             complement(20977..21813)
                     /locus_tag="IVN13_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7011277.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21810..22679)
                     /locus_tag="IVN13_000767"
     CDS             complement(21810..22679)
                     /locus_tag="IVN13_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011278.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(22749..24032)
                     /locus_tag="IVN13_000768"
     CDS             complement(22749..24032)
                     /locus_tag="IVN13_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7011279.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(24046..25731)
                     /locus_tag="IVN13_000769"
     CDS             complement(24046..25731)
                     /locus_tag="IVN13_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP7011280.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            26006..27031
                     /locus_tag="IVN13_000770"
     CDS             26006..27031
                     /locus_tag="IVN13_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7011281.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(27626..31279)
                     /gene="rpoC"
                     /locus_tag="IVN13_000771"
     CDS             complement(27626..31279)
                     /gene="rpoC"
                     /locus_tag="IVN13_000771"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP7011282.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(31358..34975)
                     /gene="rpoB"
                     /locus_tag="IVN13_000772"
     CDS             complement(31358..34975)
                     /gene="rpoB"
                     /locus_tag="IVN13_000772"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7011283.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            35703..36671
                     /locus_tag="IVN13_000773"
     CDS             35703..36671
                     /locus_tag="IVN13_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7011284.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            36689..37594
                     /locus_tag="IVN13_000774"
     CDS             36689..37594
                     /locus_tag="IVN13_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011285.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            37591..38397
                     /locus_tag="IVN13_000775"
     CDS             37591..38397
                     /locus_tag="IVN13_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7011286.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            38554..39522
                     /locus_tag="IVN13_000776"
     CDS             38554..39522
                     /locus_tag="IVN13_000776"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP7011287.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(39839..40363)
                     /locus_tag="IVN13_000777"
     CDS             complement(39839..40363)
                     /locus_tag="IVN13_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP7011288.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            40650..>41837
                     /gene="scm"
                     /locus_tag="IVN13_000778"
     CDS             40650..>41837
                     /gene="scm"
                     /locus_tag="IVN13_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7011289.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASS"
BASE COUNT        12060 a         8809 c         7695 g        13273 t
ORIGIN      
        1 taaaaaatat gcggcgcaca gaacgcgccg catatcggac taagttttat tcaaatttgt
       61 cttcgccaac acaatttgac gaagagcctt ttctttcagg aagacttcgg taatttttag
      121 gagttctacc tattctttta accgtttatt ctctttttct aagcgtgtaa tttcatttct
      181 gcgaatggat ttcatgactt cctctaccga gaaaagtacg ttatccatat ttttcaactt
      241 cttgaactca tcaatacacg ctattagagc gagcttctag ctatagacta acttctttaa
      301 cagaaaaccc ttcttccgtt accagtttta cagcggtaat tttaaatatt tcatcaaatg
      361 aacgtcttgg cataatccag atcttcgctt tctcttaatt tggtgtcaat tttagtataa
      421 cccgtccaac ttatttttta actaagtgcc cggtttttct tgacaactcc acccaaaagt
      481 tagatttttt ggtctaagtt ttagggtcgc ttcattacta gaattcaaca agagatgttt
      541 tttattttat ttaaattatg gggtcagttt ccatttgatt acaaacttct ttgttttctt
      601 tttttaaacg caataataaa ggttttaatt tcagcctttt taattagaaa atcaattttc
      661 ccaaattatt ttctttagct ttcttaacga tttctgtcgc aactaccaca tctaatacag
      721 ccgaaccaac tgttttgaag atcgtgattt gttctccact tgtccgaccg atcaattctt
      781 cattgatcag ttggcctagt tccccatgat agctgtctct ttgaatatac ccatcttgca
      841 gcggtgaaat gaagtcccca gcttctttta gaacaccgtc catggtatca aaaataacaa
      901 catctgcggc tttgattatt tctcgtggaa tttcacacat ttccggtgtg taagcaccga
      961 ctccattgat gtgcgcacct tttttgaccc actcagcaga gaatgtcgca cgttttgaag
     1021 tggtcacact tgtaatgatg tctgctcctt caacgcattc ttgactggtt tttgttgggc
     1081 gcatcgttac agaaaattgt tgcatcattt tttcagcaaa ttggctggca cgctcaaaat
     1141 caatatcaaa aatccgaact tcttctaatt tacgaacggt caacattgct tctaattgtg
     1201 actgtgcttg tccgccggtt ccaatcagcg cgccaatttt agcatcttct cttgcaagaa
     1261 gttctgttgc tgctccttga acagcgcctg ttcttaattg tgtcaaataa gtaccatcca
     1321 aacaagcgga taccataccc gtttctggat ccaatacgat catcgttgct ggtacacttg
     1381 gtaagttttt cttgatgttt tcaggataca cggaaacgat tttgacacct agcgcgcctt
     1441 cgccaccttc tacataagca ggcatatata agctttgtcc gttgcttttt ggcacatcga
     1501 tattggtgcg aagaggcaca cttgcctttc ctgctgaata caatgacagt gcttttttgt
     1561 ccgcatcgat ggcttctctc attgaaaaac ttttgataat atcttctttt ttaagaaata
     1621 acatttgaat cgctccttaa ttagctgctg taactttttt taattgactg cccaacatcg
     1681 tatatgcact gtatagtaag accaaaatca cgatccattt caggatatag gtatctaaag
     1741 atttgactag gaatacagcg accaataccc ccaatacacc gaaggttgaa gtaaaaagtg
     1801 tgatttttct gctgtattca ttaaatttaa tgaattgaac actgccgatc ggtactgaga
     1861 aggtacaagc ccccatcata ataggaaagg ctgcaactgg attcaagcct agcaagtaga
     1921 cagttaccat cgttaacgca taagaaccaa tgccgatatt gttcaatgca ccatagacaa
     1981 agagtaaaaa tcctgccaag attaattttc cgccatacaa ttcactggct gttccgtttg
     2041 atggaatcca gttcatttgt cctgcaaaaa tcaacaatgc agcaatgacc aatcctatgc
     2101 cgacaaacag tttgatcgtt ttctctggta acttcacgac aaatcttggt cctaaatatg
     2161 caccgataat ttggcagata atacaaacag ctagtgtctt gatccctacg ttgatggcag
     2221 tgatgtaaga caatgccatg acggctaccg gtactacaca ttgggtattc aacgttccgg
     2281 gtagtttttt catgctgacc catttcaact ttggataaag gacggttccg atagcaaagt
     2341 cggaaacgcc aaatgttgaa agaaagaaga tgataaatga cgtgaagggc aacagacgca
     2401 tatcccctgg ttcttctttg aaattttgtt tgtttttgaa aaggtctctt agaaagtaaa
     2461 taataaaata tagattgatt gcgacaatta gtgtcagcaa aatattccga atcataaaaa
     2521 attcctccta ctagtaaatc tttctgattc cctcaaagcc ctcaccatat tttgctttaa
     2581 gttcttcagg agattctgca atcactttta gtgtatatgc atcatgtttg acatattctt
     2641 ttgctagatt gaccatggcg tggttttttg tccaagtgac atcggttgat tgcagttctt
     2701 cgttaatggt accaaacacg acttcttgct catcggcgac gatgttgatc catcttgctc
     2761 caaagtcagc aagtttgtct gtttcaaaac catgaatata atacttttct aagggaagat
     2821 catattcatg ggtagaactg aataaaatca gtacaaactt atcaactctt ttttcagctt
     2881 gctgcaatgc ctttagtagt gaatccgtga aattctcatg ccaaacttga atgagcaaac
     2941 tttcttttgc gtttttcacc aaatgctcta ctttatccag aacatactcg ataccatcaa
     3001 ctttccacag catttcttct tcatcttttt ctttgatcat ccctagattc ttggtaagaa
     3061 aagaaagatc atttttaaca cttttttcta atttctctaa aaactcattt gccgagatgg
     3121 catgataaag tttaggttct gatttattaa cgacaatcaa attctttttt aatagttttt
     3181 ccaaaatatt atatactttc gagcgtggca cacctgagat tttacttact tcatatcctg
     3241 taagattacc tttttctaat agtgtggtat acactcttgt ctccatctct gaaaaatcat
     3301 attttttcat gatttgaata atcgtttcca ttgattaatc ctcactttat ttttagtagc
     3361 tactacggta acaactataa attctttttg attatctgtc aagagttagt tcaataacac
     3421 acatataata attagcttaa aaacaatctt tttattaaaa agaaacatta taatatgaat
     3481 gtttgtgaaa aaataataca acttagtcat tctttacaac gatttataga aaaacaacct
     3541 ctttaaatat aaaaggtacg ggttactgat tacttgtaaa tctttacgtt ccagttttat
     3601 tctttctaaa aaatatactc acttcttttc ttagacttct ttttttgatt ctcctcttta
     3661 tggtcttgtc cttttttgcc ttcaaaatat ttaaataaac ccctttcaaa agcatttcta
     3721 ggttttggac aataaggaat gacttttttc ttcatataaa tacctcattt aactatttga
     3781 tatcatcaca ttatcaaaaa aaataggttt tttattaaaa ctatcttgac ctttcgggag
     3841 atttcactgt gatttatttt tattcccata cttttttcgt tggtcacttt cgtataccta
     3901 atctaagcga tcatcggcga cggtgctttt acaacgaatg gcaggacaaa acaaggggcg
     3961 gaaaaacttt tttgcctatt tcataaaaaa ctttttccta cagcaatccc ttgttttgtc
     4021 ctaccacacg ttgtgaaccc aaagcacgcc gacgaacgct tagctatacg aaaatgacct
     4081 ccaaaaacaa aaaagtgagg tgaatagaaa caaaaagaaa ttgaaatctc ccaaaatgtc
     4141 gtccgcctcg gagacaactt gctgctgaaa atggttggtt aattcattta agtattaaat
     4201 aagataaatc atcaaattaa tcggaaaaat agcatgattg gccttatcaa aaataaactg
     4261 taaaaactta tatttctatg agtggtggaa caatgctaat accaaattaa tttatcgatt
     4321 acccacctgc acaaaccaaa gatagcatta ttaggaaacg aatcaatcag cgaatccagc
     4381 attataatga ggatactaaa aataaagttt tcataattat aaatccgcct tcctacgaaa
     4441 ggcaccaatg aggtgatgaa aaccttagag cgctttcttt ctttttttat tccgtgttac
     4501 cctttccttg caggtccctg gtacactaca gaacccgagg aggtgagtgg cggggctgta
     4561 tagcggcaac cccgcataat tatatggtgt cggtcatccg ttaaggcatc aatgaatggc
     4621 gcaaacccgt agcccgtaaa cttatctctt ccgaagttga ctcgatttcg ccggatgacc
     4681 agtccctacc aaacctgtac tattttttta caggaggttg ctatgttcaa aatctttcgt
     4741 aaaaattgtt gtggcttgga tgtccacaaa acgtggatct atgcgtgtat cggaatcaca
     4801 gatgcgaatg gtcgtacagt gtataagcaa gctcgcttct cttcctttac aaaaggtctc
     4861 aatgaattag ctgaatggct tgctaaatac aactgtaagg atgtgtgttt ggaatcttcc
     4921 ggtaaatttt ggattcctgt ttttaatatt cttgaaaaaa cttgttttgt cacactggct
     4981 catccgaagt acaccaaacc ccagaaagga aataaaacag atcgcaaaga tgcaaaaatg
     5041 gatttgtgat ctctttatgt gcgacatgat caaacccagt tttattcctg gtcttgaaat
     5101 ccgccagctt cgtgatctaa tgcgttatcg gacgaaactg accaacatga aaacaagtga
     5161 aaagaatcgt gctttaaact gcttaaccgt ttctaatctg aaactagatg atgtttttag
     5221 tgatgtgttc ggaaaatcat ccacttctat catccaatac ttgttagaac atcccggtga
     5281 atcatttgat gttcgaccat ttatccatgc ccgttgtaaa actcccgttg aagaaatcca
     5341 agcggcagtt gagggggcga tttctgtacc tcaagcgatt aagcttcgcc aatgcctcaa
     5401 ccatattaac gagattgaga agcatcgaga agagatcaat ctagagattc aagctcttgc
     5461 agaacccttc tcccctgttt tggaactact tcgtaccata ccgggcttag acactcagcc
     5521 aataacagcg attgcgattc tttcagaaat aggaccggat atgtccgtgt ttccaacttc
     5581 gaaacacttt atttcttggg ctggatgctg tccaagaaac gatcgcagtg cccgacgaac
     5641 aaaatcaaca cgtatctctc gtgcagggca gtcgttgaaa ccgctacttg tacagattgc
     5701 caatgctctt ctcagatcca aagaacatcc agaatttaaa aatcgctatc gtagaatcaa
     5761 agcgaatcgt ggacacaaga aagctatcat tgctatttgt aagatgctcc tgaccgctat
     5821 ctggaatgtc ttatcaaagg tggaacccta ttctgctaaa ggatatctgg agccaaaacc
     5881 tgcgaaagaa gagaaagttc tgacgcaatc ccaagctctg gaccttttaa gaaaacgcgg
     5941 atataccatt atagatagtg cttaaaccaa agttacgttg attcttccta aatagactcg
     6001 ttgtcttcca tttcaaaaat tttgaaaacg gtaggcttat ttagtgtacc gttcttcagg
     6061 tatcttcaaa tagatttatt ttcaaactta tttcctttct tcttttactc tcctatagct
     6121 tagcatttaa gtgggcactc gtgtcacctt gctagatagc tatgaatgcc gtaataagcc
     6181 tcctagtata taggcagact gccaattata actgattcaa tccacattat gataaaatcc
     6241 accttttcgt ttcatatgtg ttgaaatatc acatatgtca aaagtgcacg agtcttttca
     6301 ttcagtgctt tgtaaatact caaaattatt ccttttcact ttaaaaaaaa gataattagt
     6361 acaaaatcag aaatgaggaa agtattagga tggactagaa aggagttgtt gaatttgttg
     6421 caacagattg gtcatttatc aattaaaaat taggtgaagt tttattcatc actgcagctg
     6481 ctgtttccca gagaataaaa cgtctagaag aaactggtca gttactgtat tatcaagcag
     6541 caattgacta cgaacaagct tgattactaa ccaaagcgtt tatccacctg ttgattaaat
     6601 caaatcaaaa atcaggtttt tatacgttca taaaagcaat ctccagtgta ctcgaatgtg
     6661 actgtatcat aggtccttat tcgatgttaa tcaaaatggg gtttccttct accgaatcat
     6721 tggacagttt tatcaatgat ttatcaaagt ttggacgaac caacacccaa atcgttttct
     6781 cgactcttgt ttccttaatg gtttatcagt tctggaatgt ttatcatgcg cttttattaa
     6841 aatagaaaag agcttttttc atgcgacaaa gcagtgatca tttattggca catgcaatct
     6901 ttgtggatcc attttttgaa aatacgatat ttagttttga cccaaaaatc tttaaatacg
     6961 tatttgataa tttatcttaa caaccagata attttattca gcaccgtatt atctatattg
     7021 aattctcgga atcaagataa atttttttcc gtttttgttt cttttttaat cttcttcttg
     7081 ttattaatag tgattaggat gatattccta tattgatctt tgataattag atataatttt
     7141 tgaataacgt tccctgctat tcgagctaga aatacaagta cgccatgacc tagttagcga
     7201 caactactta gagatttctt ttgttctggg acaacaaaat gccatgacaa tagcagtaaa
     7261 taatgatact tccgtaaggc tcgccgtaaa ctggggagag gtgaaattcc tatgtgctta
     7321 cccaaagcgg ttcaaaaatt ttaaactatt attttaggag atgattttat aaaatcgaaa
     7381 gcctacttga ataaaaaaca agcctgtgaa tttctaggaa ttgataataa tactttagat
     7441 aagtggattc gtgtatataa ttttccagta ataaaaattg atggcgtagt tcgctttgac
     7501 gtagaggata tcaaagattt catgaataca ttcaaaaaaa attgaaattt tcttctcgtc
     7561 acatatacta taaatagcga aatgtactgc cttacttttt aaaggagtga ggaagtttgc
     7621 ctacaaagct ttcaaatggg aaatataaaa ctaatctgag gtatccaaaa aaattcagag
     7681 aaatcactgg aattgcatca gagaagtatc aaaaagtatt tccaactagg caattagcta
     7741 ttaaggccga aagggaaatg aaaaagaaga ttgaaacagt tttaagagag gagaatgcaa
     7801 actcactaga attgaaagga aaaataaaat ttaaagattt ttatgaatca aaatggcttc
     7861 ctagatatga actaggtcaa actactaggt caaacagggc tccaagtaat gtaacaatta
     7921 gtaatactaa ggatatattc cgattgcaca tccttccaat gtttggtgaa tatgcaatga
     7981 attatctgaa ttcaaatact gagataatat cagatgaatt aacaaagaaa tcgaaagaat
     8041 acgcaaatat aaaaattatc aaaggctatg ttcgctctat gtttgatatt gccgaaatac
     8101 tatgttatat cgaattcaac cgaactacca aaattattca atgtattacc gcgcctaaga
     8161 aaatggcttt agaagaaaaa aggataaggg aagggaatca agcattatca tcagaagagc
     8221 tgactgattg gatagaagca gtaaacaatg acttaaataa tcacttgctt acacttcacg
     8281 attacacact tttcatgctt accttatatc taggtgatag aaaaagtgag acttatgctc
     8341 tgcaatggaa atatattgat tttaataaaa atacggttcg tctcaaatat gctttagata
     8401 aatatcaaag aaagacacat acaaaaggtt ggaaagacac cgttattcag gcaccagaag
     8461 ttgtgatgac attattaaga gaatggaaat cagctcaggc tgaacaattg ttgaaactaa
     8521 aaattataca aacacctgac cagtacctct ttacctattc caaaccttct ggagaagtaa
     8581 attgtcctgt tcacgctgac tacttaaact atcgaatcaa tgcaataaaa aaaaggcatc
     8641 ctgaattggc tcaattatct ccacacaaac ttcgtcatac ctatgcaact atagctaggg
     8701 aaggtggagc aaatatgaat caaatctcta atgctttaac gcactcggat atttctacta
     8761 caaaaatata cgtgaacaca cctgacgtag ttgataaatc tgtttttgaa gcatttcaaa
     8821 aaggattgaa taagtgtgat taacatgtgt tctcaaatgt gttcttatta taaaaaaaga
     8881 cactttccaa ataattgaaa agtgtcctga aatattgata taaaaagaat attaacgttt
     8941 tgagaattgt gaagctttac gagctttttt aagacctggt tttttacgtt cgaccatacg
     9001 tgcgtcacga gtaagcaagc cagcacgttt aagcgctaaa cggaagtcag gatcaacttc
     9061 tagcaatgca cgagcgatac catgacggat agctcctgat tgtccagcgt atcctccgcc
     9121 gtttacgtta acaaatacgt catatgcgcc ttttgtttca gtaacagcga aaggttgatt
     9181 gattacttca cgcaagtcag catgtggaat gtattcttca acatcttttt tatttacagt
     9241 aattttgcca gttcctggta ctagacgtac gcgagcaact gcgtttttac gacggcctgt
     9301 gccgatatat tgaacttgtg ccaatgaaat tccctcctat tagattaagt ttgtgatgtc
     9361 taaaacttct ggttgttgtg ctgcatgtgg atgttctgct ccaccgtata cgttcaattt
     9421 tgtaaattgt ttgcggccta aagtgttttt aggaagcata ccttttacag aagtttcgat
     9481 caaacgacga gaatttttag cacgcaattc gccagcagtg attgatttca atcctcctgg
     9541 gtagttgctg tgacggtaat aaattttgtc agtcgctttt ttacctgtta atttcacttt
     9601 gtctgcattg atgacgatga cgaaatcgcc agtatccaaa tgaggtgtga aagttggttt
     9661 atttttacca cgtagtacag atgcaacaac tgttgaaaga cgtcccaatg gaacatcagt
     9721 tgcgtctact acataccatt tacgttctac ttcgcctggt ttggccatat atgttgtacg
     9781 cacgttattt tcctccaatt taagttctga tgtttgacat acacttcgag tttccggggc
     9841 tctttgtggg gcaaacaata ccatttaaca tattacccgc taatctagtc aatgtcaacg
     9901 tttttctgct tgattgaaaa atttatatga aactttactt ctttttcttt cttttatata
     9961 aaagggtcgt ttcctgttaa aataaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac
    10021 aacctcttat tttaaataaa cggcggtctc ctgccaactc ctcggtcaca agtcacaata
    10081 tccataaaac ttaagaagct gttttttgaa attgtttctt gtgactcgga gctgaacggc
    10141 aggtttacga cctcttggta aacggtgttc aatcagctga ccctcggtat tagctcaaaa
    10201 aacggcaaaa tatgaggaac tattttgacg tttgtttctc taatactcag gtcaaaacgc
    10261 tgatttcaca acctcttttt cggtcaagtt caacaacagt ttttacgcgt cgatctctgg
    10321 cgctttgccc aaatgagctt ggatcatcca gatacgtttt tctgtggctg ttttgaattc
    10381 gataaagata tcttgtgttg gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg
    10441 gtaaagatct gctagataac gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt
    10501 acgatcccag cggccaattt catcagggat tttcgtgttg tctgcaaatt cacgcaatgt
    10561 agaataaggt gaaccgtcta atgtgatcaa gcgttcggat acttcatcta actgagcgtt
    10621 caaatcatcc atgaactcat ccatcattgg gtgcaatgta aggaaacctg gtcctctcat
    10681 gtaccaatgt gtttggtgaa caacaacaga catttggctt agatctgcaa ctaattgatt
    10741 taatacttct ttggtttgtt ggaatttcat ctttcttgcc tcctaacatt ttcttacact
    10801 taaaataata gtagactttc aatgaaaagt acaacaaaat catttacctg agaacgattt
    10861 tcagttcgct tttttatccg taaagccaat catttttcag aatcagctaa ttcttctcgc
    10921 agttgagcaa attgctgata agtctgtgct aaatgctttt gtgtcgaaac aaataatgga
    10981 tagattttct gatatttttt atgatcagtt tcttgtactc caacttgctc tttcaattct
    11041 atcaattcct gtgcttcact gatactttgg atctttccga tgcttcgcca gcctaaaatc
    11101 acagctccta atgcggatgc ttctgtgaca gtagggaaaa caagcgggta acccagtaca
    11161 tcagccatca tctgacgaaa aatcggagat tgagcaaacc cgcctgtagc acggatctct
    11221 gtgagctgat tttcctggca aactcctttt aaaatccgat aaaggttgag acagatccct
    11281 tctagaatcg ccttcatcat ttctttttct gtatgttgcc gctgtaaacc gataaaactg
    11341 ccaaacgcat ctgcgtccca aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc
    11401 agaccattgg cacccgctgg cacaaaagcg atagccgaaa gcagtgcatc atatggattt
    11461 tctccttttt ctttagcaga ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca
    11521 aagatgccag caccattgct agtcgctcct cccactaccc aatgtttctg atccagaaca
    11581 taacaaaacg tctccatttc tggatggaac tgaggctgag tagtaataaa acgaagtgct
    11641 ccgctcgttc ctacggtcaa agcggcgatc gtatcaccaa aagcacctaa ccctagatta
    11701 gaaagcggcc cgtctgcccc tcccaaaaca aatgatatat catttggcaa acctagttct
    11761 gctgctgttt ttgatggtag acctgttact tgataggtgg aaggaaagac ttgcggcaat
    11821 tgctctattc gaatgttcaa gtagtcaagc acttcttctg cccattggaa ttcatggata
    11881 tcaaaatatc cagttcctga agctgttccg tagtcgctga tcagttcgcc aaacaatcta
    11941 taaaagatat agtctttgat accgacaaaa taagcagttt ttttgaataa ttcaggttgg
    12001 ctgttttgca gccacttgat tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc
    12061 tgccgataaa attctttgcc aagcacggta tatttcagtt tttctgcttc gtccgctgct
    12121 cggttatctg cccaagtgat cattcgagat aacggtgttt tctcttcatc caacaatatc
    12181 aggccatgca tcgcagagga gaaggagaca aattgaatga ttgtgtctga aggcaactgt
    12241 ttaattactt cttttataac ggtgcagaca ccttgaaaga tcaattcctg atcttgttct
    12301 gccatccctt gtgtttcttg gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg
    12361 gcttcatctg tgaaagcaac tgctttggtt tgtgtggttc cgatatctaa tccgattgaa
    12421 acgttcattt tttcacctca aattttatgt atgtacaaaa atttagttta agcttgcgta
    12481 tcttggtttt gaaaaggggg agatggcgtg ttactttatt ttatcattgg ttgctgtatc
    12541 ggatcatttt tatgtttaac cgcccagcgg attccgttag gtcactctat tatttatcct
    12601 agatctcatt gtgtcaaatg ctgtcgttcg ctttcttggt atgaattgat tccgattctc
    12661 tcgatcatcg tccaacgatt tcgctgccgc tattgcaggt gtcggttgcc cttctactat
    12721 ttgttggcag aagccttatg tggtggtctt tttgcctggt tctttacttt ttcttccgca
    12781 agaaattttt cgacgttcat atggatgctt tcggctttgc tattttcgtt gatggattta
    12841 ttttacttca tggtccattc tcacacgcta ttctgttctt gggcaatttt atggattttt
    12901 tggttgcagg ccggcgtctt ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt
    12961 gtgtgccttc gctatggtca atcctatctc ggagctggcg atactctgtt gatgctttct
    13021 tggagtggtg gcttgtctct agaagagttg cttcagatct tgtttcttgc tagtctgttt
    13081 ggaatatccg ttttttctgt ttactttatc tgtcataaaa aaaagttgga aaagctgcct
    13141 ttcattcctt ttttgagcag tgcgttgctg attgtccttc atcttaggta ttgaaaaaag
    13201 gttgtgacaa acgacttgtc acaacctctg atatatagtg ttctcttacc aaatccttgt
    13261 gacttggagc taggcgacag gttcacaacc tctccactaa atggtgttca atcagctgac
    13321 cctcagtatt agctcaaaat gctggtttta caacctctga tatacggtgt tccataagta
    13381 gctttttcgg cttcttattt cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca
    13441 gaaccttctc gataaagatt gtaatggagc ttgtttttct tgtaataatc ctgatggtaa
    13501 tcttctgcag gataaaattc gcttcttggt tcgatttttg tcacgattgg cttatcgaat
    13561 cgcccgctgt cttgcaatgc ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca
    13621 ttatagtaga tcactggacg ataagaatca ccgcgatcag cgaactgtcc taatgcgtca
    13681 gtcggatcgg tttgctgcca atagatttcc accaactgat cataagaaat aacttctgga
    13741 tcatattcga tctctactgc ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa
    13801 gttggatttg gtacatgccc gccagtataa ccggatgtca cagaaatgat ccctggctgc
    13861 tcttcaaatg gtttgaccat acaccaaaag catccgcctg cgaaaactgc tttttttgtc
    13921 atataaaatc cccctttttt ctcatcatac cttgatttcc aaaaggagga taacgttttg
    13981 cttgtgacag aaaaaacgga aactattata tacgtgatac ggcttctgat aaatgccaaa
    14041 acgaacgtac ttgatcaaaa acggttaaat caggaatcac atattcagca gaaggaagat
    14101 ttatgttttc acctattccc aatacatgca tgttaccagc caaagctgct tgacaaccag
    14161 cttctgaatc ttcaatgacc aagcatgcat ttggcggtac attcaattgc tgtgctcctt
    14221 tgagaaaaac ttctggatca ggttttgctt ttgatacttg cgtgccgtca ataaggacat
    14281 caaaataact tgtcaaattc gttttttcta aaatgaggcg tgcatttttg cttgctgatc
    14341 ccaatccgat cttgatgttt gtttttttca agtactcaag aacagctatt gcaccaggca
    14401 aaacggatgt ttcatccatt ttttgaatcg cttgaagata tagattgttt ttttgctcag
    14461 caaaggctga tttctctttt tctgagaatt tctgttccat ttttccaaat gacagcaatc
    14521 gctctagtga ctcggttctg gatattcctt tcagttgctc gttttctttc tcagtgaatg
    14581 gaatggacag ctggttagct aaaaccagcc acgcttcgta atgataacga gctgtgtcta
    14641 caagaacgcc atctaaatcg aatagtgctg ctttgatttt catcgattac ctccttgaaa
    14701 atgaacgact tgaagtaaag atgcgatgtt tttagctgtg cgatgaagat tatcagtggc
    14761 ttgctgtaaa gcttcttgta aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat
    14821 gccaacatct gctgcttttt cgcttccttt accaacactt ccgcaaatag ctatcacagg
    14881 aacgccctct ggtactcttc tagcaatccc aactggcgct tttcctttta gactttgtcc
    14941 atctagacgt ccttcgccta cgattacgag gtctgcttgc tgtacccgtt tatcgaactc
    15001 cagtaaatcc aatacgtagt cgatgccaga gactattttc cctgaagcaa atgctaacag
    15061 tcctgcaccc attccaccac cagcactact gcccgatgcc tcgattactt gaggggcaaa
    15121 tctcatataa aaatcatgca tggcttggtc agccactgct aattcatttt ctttcaagcc
    15181 tttttgtggt ccgaaaacaa aagaagcgcc ttcttttccg cataaaagat tagtaacatc
    15241 agtaatgaca gtgattttgg ctttaatcag ctcaataggc acgttcgttt tatcaactgt
    15301 agcaatttta cctagatttt ctcccagtgc ttctactttt tgattatctt gatcgtaaaa
    15361 agcataacct agaccttctg ccatgccgat gccgccatcg ttggtactac ttccgccaac
    15421 gccgatgatg atgtgtcgaa caccttgttg caacaattgt cgtatcagtt ctcctactcc
    15481 tcgcgtggat aacctaagtg gctgttgttg gtttagtggg acatctttca atccgacgat
    15541 ttctgccatt tcaaaaatcg cagtttgacc gtctgaagcg tactggattt ctttgttttt
    15601 gccaaatgca ttagtaacga ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga
    15661 tgaaaccgtt ccttctcctc catcgcctat aggtagcaga tcatattcag cctgaacaaa
    15721 aacttcttga aaacctgatt gaatggcttg tgctgcctct aaggctgtca gactttcttt
    15781 gaaagaatct ggcgcaatca gaatacgcat tattttcccc tccgttccaa gatcagttct
    15841 ttttccagct ggtattggtc tccattgaca aaaataggta atgtttcttt cgtctctttt
    15901 tttattacta tttgtttttt tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa
    15961 ggaaaagaaa ttttttccca tgctgaagga agtcttggag aaatagaaag agttccattc
    16021 gttactgtaa tacctgaaaa accaaatata actgctagcc atagtgctcc taatgaagca
    16081 gcatgaattc cgtcatctga tgaatgcgga tctggtccta aatcaatcag gcacgcttct
    16141 tggaataaat gatacgccca ttccatttct tggatacggc aagcctctat ggcgtgaatc
    16201 gctttactca acgatgagtc gtggattgtt ttgttttcat agtaatgcaa attcttagaa
    16261 acgattggtt gagaaaaata ctcaggtaat aagtataaca gcatgacaag atctgcttgc
    16321 ttgctgactt ggagttcatt tacttcgcta cgggcataat caagtaaaat agactggctg
    16381 ccaggacgtt ctttgtaagt ggtcaaatca ataactggct tactgagata cgtgtcatct
    16441 tgcggaatca gctgttcttt gttaggcact ggcaaatata acttatctaa aaaattttga
    16501 cattctttca aaaaaagcgg atcgccagct tgatatttaa cagctaaggc cacattataa
    16561 tgcgccagat agttcgtata tgcattattg tcgatatatt ctgtgtattc atctggacca
    16621 atgactctgt gtatctctaa atgatcatcg acttttacag ctctgctcag ccaaaaagct
    16681 gctgtttcat gaatgagcgt ttttcccttt gtttctaaaa acgattgatc ctctgttgct
    16741 tggacatact cttgaacggc ataagcaagg tctgcactga tatgatgttc tgcggcagct
    16801 gaagcaactt cttgtcttgt tccggttttg atattcaatg cagcaaattt gggtgtctct
    16861 tcctgaccag tgtgtgcgct ttcccatggg aaaagtgcac cttgatagtg ataatctctt
    16921 gctttttgcc gtgcgccttc taatcgatca taacggtaca gcagcaaatt ctttgctact
    16981 tcaggaaact gatataaaaa gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc
    17041 cctttataac cttcgccagt tagtcccttc gcccctacac taacacttgg atcattgcgg
    17101 ggagtcataa tctgtaaatg gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa
    17161 tcgccttgga tcgttatccg atgctgatgc caaaaatctg tccaataaga catcgactgc
    17221 tctaaaattt ctgtataact aacgcttttc tgggcttggg caatactttt cttaaacggg
    17281 tctgtttgct gaagagaggt agagagcacg atgatcttct ccagcgtaaa ggacgtattt
    17341 tttctgatct tttctgaaaa ggaagcggtg atccggcgat tttttgcttg gaatacacct
    17401 gctttattga atttagctgc aatcccaata tcaattttac tttctgttgt ctggtaggat
    17461 gcactgatca aatcgtcacc aaatactcgc agttcttttt caaccaactg ctgtgtacca
    17521 aaattggttt gctgtccatc gatacccgtt ttcacatcta cttggcagtc ttcggtcaac
    17581 ggcttgatga ccactttcag ggctgcctga tggagattat tttgatcagc aacacgacaa
    17641 gtaatgactt ccactttttt tccttgagca gtttcaatga gcgcatagcg tttaagctct
    17701 cccatttcta aatctagtgc ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa
    17761 tgttcaccgt cgatttttat ttcccatctg atgacatctg ggagattcac taactcggct
    17821 gattctgctc ctaaaggtcg gttatataca ccagctgcaa acattcccct tacttgattc
    17881 cgatagtctt cctcctggct tcctctgata ccaaaaaatc catttccaat ggtcatctga
    17941 ctcgcatact tattgatata ctctaaatca aactgttttt cttctacttt cataaggtaa
    18001 taacttctcc tgtttctgcc gaacgatata aattttccac cagattctgg atgacatatc
    18061 cttgccagcc atctgcaatc atctgagtct ggtcaccgag cgcccgattc acaaaatgag
    18121 ccatacttag ttcatgtggg tcattaactg gcgtggtctt ttgtaaggaa accagctttc
    18181 catctacatc ttcgaatagt tcgcctggat acagattagc acctgcctga tcgccacaaa
    18241 aagccacatt cagtttgtct tctctgatat tcaaagcaaa cgaagtattc agttggatga
    18301 tcttgccgtt ttctaattcc agtgtgccaa aaattgcatc ttctactgtg aatttgcttg
    18361 gatcccattt cccgaatttc cctgaatttt ttttcgtacc aattttttgg aagctgtacg
    18421 cccgaacgct ttttacttta ggaaatccag taatatacat cgctgtatca agcatgtgaa
    18481 tgccataatc gatcaaaggc ccgcctcctt gaagttcttt gtcgataaac gttccccagc
    18541 ctggaacacc agaccgacga agcgcattcg cttctacgta ataaatatcc ccgagaacat
    18601 caagatgatt cttgatcatt gttgcttcgt cagaaaaacg atgctggaat ccataagcca
    18661 aaatctgccc gtttttatcg gctgtctctg ccatctttct tgcttgatca gctgtcatag
    18721 ctggtggttt ctcgcacatc actgaagcat tagcttcgag ggcagccaat acatgttcga
    18781 agtgaaagcg attgggtgta cagacactca cgatatcggg agaaatctca ttcagcattt
    18841 gtgtaactga tgtatacgca tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct
    18901 ggctgactgg atcaaccaca gcggccaatt ctaagacatc tgatcttttt tgataataag
    18961 cagcatgtac tttttctgct acttgtccag cgcctatgat tgctactttc ttcatttatt
    19021 tctctctcct ttgcttattt acttattgtt attctttctc tattctttct ctaaatcatt
    19081 taaatgacgc gctgtttcca gacagttttt caagtattgc aatgattcct gataaatttt
    19141 cacttcattt tctcctcgca aacgacattc atataccata taaccttcat actcattttg
    19201 aagtagcgtt tgaaaatggc gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg
    19261 attatctgct aaatggatat gacccacata ctctttttgc gtagtcaatg cttttgaaat
    19321 atcatcttct tcaatattca tatgataaaa atcagcgatg atctgcacat gtttcaattg
    19381 attagagtca atgtaatgac gcgcatccgc taacaaattg atcatatggt cttggtatcg
    19441 gttcaaaggc tccaaataaa taaccgtacc tgtttcttcg gctactttat ctaaataacg
    19501 aagtgattcg ctgactgctt tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg
    19561 taatcgataa gtgaacattc cccaagctgc tgggataacg attcctttgc ctccaacctc
    19621 gcttagtgca cgcaaaatct cagcaatttg acgtacacca ttaagacgcc gttcttcgat
    19681 aaagtcaccg atccacccat cgtagccgtt acaagctgtc actactggga tccctgtttc
    19741 tttgattgcc tgtttgactg tgtcgatatt ttctattaac aacttaccgt cgatttcgta
    19801 ttcttcaaat ccacagtctt tgataaatct aaatttttct aaaatgtctc ttgggaaaaa
    19861 tgcttgatct tgcgtaccga atttcattgt tctcctcctc ggacttgaac gcccattttg
    19921 atactttctt ctgggtgctg gtctacataa cgcatgtaag cttctgcgct ttctgcaaat
    19981 ggcacaactg gatcaatgat ttcttcacaa tccaagtaac cgttcatcaa taattcccaa
    20041 catgtgtcct cgattcgttt acggttccat cttgggtaat ctgggtttgg ttcactagat
    20101 gctcgagcaa agacgatttt tgcattattg aaatgtgctt cgcgaccaag attaaaccca
    20161 gctggaaaag gtttagcaaa ggcaacataa gaaatgatcc cgccatagcc gatccctatt
    20221 aaagaagctt gtaatgcggc agcactgcca ctcgtttcaa taactgaatc tactccacgt
    20281 ttatctgtca attcctttag acgaaaaccg acatctgctt tcataggatc tagcgtgtaa
    20341 tcggcaccgt gtttttctgc taattctcta cgctgcgcta tgggatcgat cccaacgatg
    20401 gtagtagcac ctgctttttt agctaattga atcgcaattt gcccgatagc gcctaaaccg
    20461 aagattgcta cagcatcacc tgctcgaaca tgtgcttccc ggataccacc taaagcgaat
    20521 tgagcaggat cgtaacatac tgcatttttc cattgatcca accgatcaag tttacgtagt
    20581 ttataattat caacaccgtt tgtaatcagc gtttcacgaa tgggtccata agtgcagact
    20641 aactctcctt tttgatactc tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc
    20701 atatttccta attgaaattc gccaaatacc acaccgcgtt ggcttccttc ttctcttggc
    20761 aagaataggc gccattcttc tgaaaattct tcatcgataa atggacttaa tcctctaaag
    20821 tcgacaattt ctgttcctgg ttttggagta gcgaaaatta ctcggatacg tacttcgttc
    20881 ttttcgatct cacggtcttc gtaatctttg aatccggcta ttcttggttc taatgcaact
    20941 aattttttca tcttttcttt cctctttctt ttctttttat ccttttacgc cgccttcgac
    21001 taaatcacct ttaataaatc gttcagataa cgcatacata atgatgactg gtaaagcggt
    21061 caccaatgaa gcggccatca ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt
    21121 caaaccgatt ggtaaagtaa atttttcgga ggtcgacagg aaaatcgaag caaataaata
    21181 atcattccac gaaaccataa agcaataaac gaagacagaa atgattccag aaatagccaa
    21241 tggtataatg atccgaataa tgatctggat ccggctaaga ccatcgatca ttgctgcttc
    21301 ttcaatatca gtcggaatcg tgtcaaaata actttttagc atgaatatcg cagtaggaag
    21361 tgtttgaacg accatcgtga taatcagtgc tgtattggta tcatacaaac ctagaccgga
    21421 aatgatttta aataatggaa caacgatcaa aataccggaa aacatataaa cggtgtaaaa
    21481 gctagcattg attttcgagc gagctttgaa gcgtagtttt gacaaagcgt atgccccgct
    21541 gatcccaatc actactgcag ttacggaggc tatcaaggag atcactaaac tatttttgaa
    21601 atacatgatg aatggaaaaa tctcagcatt gaaaatatcc tgataatgtt caaaagtcca
    21661 ttctttcgga aaaatagttt gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt
    21721 catcagcatg atcaaaaatg gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta
    21781 aaataagatt ttcctgctct ttttattttt catttcaagt ccaccttctt tcgtgagagc
    21841 aagacgaccg ctgcaataat gatgaacaat agaatcgata tagctgctgc ttttccaaga
    21901 tcattgaacg caaaggctgt ctgatacaaa taaacgccta agatattgac tttattcgtc
    21961 agtagataga catctgtaaa catatagaac atccagatag atcgtaaaac aatcacagtt
    22021 gctagtactg gtttgattgc cggcagtgtc acgatcttga acttctgcca agcatttgca
    22081 ccgtccattt cagctgcttc ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat
    22141 gaaataaacg catacggata gtatctccag atagcaaaca aaacgaccat aaagaagcta
    22201 gcaactggct ggtcgaacca cagcggtgct tcgttgaaaa gctgcaacct atctactaac
    22261 agatcattga cgattccata actattattg aacatatatt tccaagcaaa aatcaaagaa
    22321 atagatggcg taacataact taataaaatc agtgaacgca ccgttttcct aaatctgaac
    22381 ttgcggttaa gcaaaatagc tacggctaat cctaaagctg tactgcctgc tacaactaag
    22441 aatgtgtaag caactgttaa acctaaagaa cgataaaatt cactatcact taaaatttca
    22501 agataattgc ttaatccgat aaaaacggcg tctgagttag gattcagcgg catatcgaaa
    22561 aaactgatct gaatattgga aagcattgga atcagcacta aaacagctaa taaaagcatg
    22621 cttggtgcta aaagtcccat ggctaatttt aagtcagaat gtttccttgg acgattcatt
    22681 gaaacctccc cctgataatc tgccaatagc tatctacttt tttcgaacac cattggcaga
    22741 acttcttgtt atttcgtatt tttttgtgcg tcagctaaag cttgatcgac atcttcgttt
    22801 ccgacagtta cttggttcac tgcttttccg atcacacctg agcttgtgat atctcccatt
    22861 ttcgtgaaat tcttttcccc gactagtcca aagacttgta cttcatcaaa agcactagca
    22921 atctcagatg gcagctcgcc aaaagacttg atgacttcat tttctttgta accatccagt
    22981 tcgactactt gtttattcac tggttgagca ccaccaggag acatcaagac ccacttttcc
    23041 atgtttttcg gttgagataa gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg
    23101 tctagaccag aagaaatcgt caatccagat actgtgccat agacagcttg atctttttgt
    23161 gttgggatag caaaaccgat attttctgag ttccccgctt cataaacaga aggcaaaatg
    23221 taagttgaat agattgccat tggtgctgtt ccattcatga aagcatcttt gatttctgtc
    23281 acatcattag agcctggcat cgtgtattgg gataagtttt gataaaacga caatgcttct
    23341 ctcatctttt ccgtatcgat cgttacctcg ccttcatcat ccaaaacatt tgctccattt
    23401 gacaaagcaa attgagagaa cgcttgctca ctcattgttc cttcagcagt aggcatagca
    23461 attccatatt gtttattctc tttatctgtg aaatgttgag caatttttaa aatatcatcc
    23521 caattttctg gttctgaaaa accagcttct gataattttt ctttgtcata ccaaatacct
    23581 tgaacccagc cgctaattgg tgcagcaata tagctttttc catcttctga acgaacaagg
    23641 tttttggctc cgtcataata attatcttct ccagcttcct cgataacgtt ttggactgcc
    23701 gtctgatcaa tcagttcatc tttatccatc actttagcaa aatcctgact gacttccatt
    23761 acttctggta atttaccgga acgagctaac gtaacgattt ttgtattgaa cgctgattct
    23821 tctaccggca cttgcttgat tttgatgtct ggattttctt tttcaaaatc agcaaccagt
    23881 tcattgatca cacttaatcg ttcctgttcg acagaagagt gcatgaattg aatcgttgtc
    23941 tgttgtgatt cgttagagct ttgactttca ccacaagcag taagaagaaa tccagtagct
    24001 agcagacagg tagtaacaat aatttttttc atttttttcc acctcttatt ttattatttt
    24061 cagccacata tattcatacg gctggaatat caactttcgt tcttgactgc aatggataat
    24121 ttgtccagaa aacaggtcta ggtaatctcc ttttggtaaa agataacctg ctgattgatt
    24181 ggaaaaatta tggataacta agactttctc ttccttcgct tcgcgaataa aactgataaa
    24241 agaatctgct gagtccagta agtaaatcaa actattcgga tggaaggctt tttctttttt
    24301 tcgaatagat agcatctggg tcaattcacc atatactgct gttcttaatg gatctgtctg
    24361 caactctttc tcaatatctg ctaacaaaaa tttttgccga ttgatttttc gattctctcc
    24421 tgttttttct acgcctttgt aatcatttcg acttcccagc acactttgaa tataaattgc
    24481 cggcacgcct ggtaacgtta ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca
    24541 ttccgtcgta tctgttggag aacttaacgc atctagatac gtagcgttta tctcatatgg
    24601 actggtcgtt ccatctgggt ttttcttgta gtttactcgg gcaccctctt tcttcagctg
    24661 ttccactaat tcaaggatcg ttttttccgg cagaatacct cgtaccggat tcaatccgat
    24721 accgtcatgg gaggctaaaa aattaaaaaa agttgttttt ttcgatgcga aatcgatggt
    24781 ttttaaccat tctttcaact tacttgtatt acccgaacga atcgcttgaa gggtcaaagg
    24841 tggtagcgaa aactgataga ccatctgtgc ttcattctcg ccattcccaa aataactgat
    24901 attatctttg tgtggcacat ttgtctcagt gatcaatacc gttcctggag ctgcttcatc
    24961 agcgattgca cggaataatt ggatgatttt atgcgtttct ggcaggtgta tacaagaagt
    25021 tcctagttct ttccataaaa atcccacagc gtccaagcga ataaatgaag cgcctttttc
    25081 taagtaaaac aaaagaatat cgatcatttt caataaaact tctggatttt taaaattcaa
    25141 gtctacttga tcttcgctaa aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat
    25201 gaacgttgta agtaacgggc tggtccttgg tctagtcacg ctgcttgtat ctgtatccag
    25261 tggcaaatca ataaaaaaat ctttgtacgt atcattcaag tttaaatatt cctgaaacca
    25321 agtactttcg gcagacatat gattgcagac aaagtcgaac attaaccgtg tttcgcttct
    25381 catttcttct atatcttccc atgtgcccaa acggtcgttg attgcctgat aatttacaac
    25441 ggagaaacca tcatcagatg tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt
    25501 atctgccaaa tacttttgat aaaatttctt aaatgtacgc aaagttggtt gctgctcttc
    25561 aaaaaattga tccccataag taatcaagaa aatatcttct tcgtcccaat gcagtttgcc
    25621 tgcctggcct ttcttctgct gataaacact gactttttgt tcaatcatag aggcggtttt
    25681 ctcagacaaa tctgagtcgc gataaatcgc ttgtaaaagt tttgtataca tcggattccc
    25741 ctttcttttt tgggagcgtt cccaacgatt tgatcatatt atggaaaacg cttactgtca
    25801 atagcatttt tttaaataat tttgatatac acaaaaatac aaataagaaa gcttatgtat
    25861 caataattaa tagcttctta aaaaaatatg gtctctcatg atcatgcaag ctattcctaa
    25921 attttcaaaa tcatagacaa aaaagaatgt ctattgtact atggaattat tctaaaacgc
    25981 tcccaaaaag gaggtgcata taaaattgaa acctactatt tatgacattg cgaaaaaagc
    26041 taatgtctca aaatcaactg tctccagagt attaaataac cagccaaata tctctgataa
    26101 agcaaaagca agtgtacttc aagcaatcaa agaattgaat tatgaaccga gcaaaattgc
    26161 aagaggactg tcaggaaatg gttttgacgc gattctagca ttatctcatc gtacttctca
    26221 tacgacctat ggcaatcctt ttttttcaga tgttattcaa tccatttctg ccgtttctga
    26281 acaaaataac ttcgacttga ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa
    26341 atgcgcgaaa aagatccaag aaaaaatgat ccgaggaatt attattttaa gctctacgat
    26401 ggatgaaaca cttctttttg aattggatca atacaatatt cctattgtgg taatcggaaa
    26461 agtcggaaac gactacacga acatctactc agtggacacg gataatttcc aggatagtta
    26521 cgatttggtg gaaacactga tcaagaatgg acaccgtcat atcggctgtc tccatgctcc
    26581 gacagaagtt tatgtatcca atgatcgtct caatggttac cgtcaatgct tatttgatca
    26641 ccacctagat atccgtaatg aatggattat agacggcggc tttcgcttag aagatagcat
    26701 ggcagcaatg aatgatttaa tgaagctacg agaaagacca actgcgattt tttctaccga
    26761 tgcattaaaa tcattgagta tttacaaagt ggcagaacaa tctggaaagc agatacctga
    26821 tgatctttcg atcgcaggat tcagtgatcc cttattgtcc tccctatttt cgccacagtt
    26881 gactcggata gacattccga caaatcagtt aggaatcaaa gccacagaac ttttattcca
    26941 actaatccac caccaatacc ctgaagaaaa aagaatcatt ctttcaaccg agattactct
    27001 cacagattcg atccaacaac aagaaatgta gaacagaatt ataaaagaac agaaacactg
    27061 aggataagtc agttactctg ttcttttttc aatcactcat ccctcgttac cccctcccgg
    27121 tattttagcg aatttttctt attttccatc tgttttagat agctgtggat aactataatg
    27181 tttgtgttaa actatttttt ccacagggaa gaaaaaacta tttttaacca cgttgtgtat
    27241 aagtttttat agttcgtttt tttaaacaca tttaccctta tttcaatcca tgttatttgg
    27301 ggatacttat ctctaaaaca taaatagaat aacagacatg tctactggtt ttgttttatt
    27361 cgaggaatta ttcaataata gctagcaaaa tattaaaaat aaccgctcta tcttcccatc
    27421 aatttatttt gcattcaaaa aatgatctat ctttactttt gttgattgac ttctcttgga
    27481 agatgatcgt tccgatacct taattggata cgaaccgatg ttgttaagat cctttcccac
    27541 ttttcttcta gttagacaaa ttattttaga caaaccaaaa aagaggttgt gacaataatt
    27601 tgtcacaacc tcttttttaa aatatctatt ttgtttgatt gttcattgcg tcttctgcag
    27661 ccatttgcgc ttcaatatct gaaatactgt agacattttc gcttgctaca ccgacttctt
    27721 ttggttccat gtcacgataa cgttgcatac cagtaccagc tgggatgatc ttacctataa
    27781 taacattttc tttcaaacca agaagaggat ctttcttacc gcggatcgca gcatccgtca
    27841 agacacgagt tgtttcttgg aatgatgcag cagataagaa actatttgtt tctaatgatg
    27901 cttttgtgat acctagtaag acaggtcgag aagttgcagg aacgcctcct gagactagcg
    27961 tattgtagtt ttgatcttta aagtctgcga tgtctaataa tgtacctggt aagatatctg
    28021 tatcacctgg atccattaca cgaactttac gcagcatttg acgaaccatt acttcgatat
    28081 gcttgtcgcc gatttctacc ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt
    28141 agttttcaac agataataca tcgcgaactt gcaacaattg ttttggatcg attgatcctt
    28201 ctgtcaatgg tgcaccacgg tggatcatat cgccttctgc tactttcata cgtgctgtat
    28261 aaggaacagt atacgtacgt gtatctgttt ttcctttgat tgttacttct ttggtacgtg
    28321 ttgcaggatc ttctgagata tcgatgacat cccctgttac ttctgtgatc actgcttgcc
    28381 ctttcggatt acgtgcttca aagatttctt ggacacgagg aagaccttga gtgatatcgt
    28441 ctccggcaac cccacctgta tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga
    28501 ttgattgagc ggcgatcgta ccaactgctt ctccgacttc aacgtcagag ccagttgcaa
    28561 ggttgcgtcc gtaacaatgt ttgcagacac catgttttgt attacatgtg aagacagaac
    28621 ggattgtcac tgtttcgaca cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca
    28681 attggtttcc tgcaatgatc aattcatttg tttcaggatg acgtacttct ttacgggtgt
    28741 aacgtcccac taaacgatct tctaatgatt cgatgatttc atttccctcg cggatggctt
    28801 ggatttcaag acctcggtca gtaccgcaat cttcttcacg gatgattaca tcttgggcca
    28861 cgtcaaccag acgacgagtc aagtaacctg aatcggcagt cttcaaggct gtatcggtca
    28921 tccctttacg agccccgtga gttgagataa acatttccaa gacggatagt ccttcacgga
    28981 agttcgaaat gattggcaat tccatgattc gaccattagg agcggccatc aaaccacgca
    29041 ttccggcaag ctgtgtaaag ttggaaatgt taccacgagc tccagaatca gacatcatga
    29101 agattggatt tttcgcttcc aaaccttcca tcagtttttg ttggatgctg tcttttgcag
    29161 cattccaaac agcaatgaca cgttcataac gttcgtcatc tgtgatcaga ccacgacgga
    29221 attgttttgt aatagtttca acttgtttat gggcattttc aatgatttct tgtttttcat
    29281 tcagtacact gatgtcagcg atccctactg tgataccggc atgtgtcgaa tgtttgtacc
    29341 ctaagtcttt catacggtca agcatcttag aagtttctgt tactttgaat cgtttgaaga
    29401 cttctgcgat gatgtttcct aagtttttct tcttgaacgg taatactaat tcttgctctt
    29461 tgatatgtgc aggaatatca gtaccagctt caacaaagta tttgtccggt gtttggacgg
    29521 tcaagttgaa gtctgttggt tcattcagat aagggaattc aggaggcatg atctcgttga
    29581 agatgatctt accaactgtt gtgatcatcg tacgttcttt ttgccattca gtaaatggtt
    29641 tttcacctaa tgagttaggg ttcacaccga tacgtgaatg taaatgaaca tatccgttgc
    29701 gccatgcgat aacggcttcg ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt
    29761 cttcttccat tgtcaagtag tagttaccta gaaccatatc ttgagaagga gtaacaactg
    29821 gtttaccatc tttagggttc aagatgtttt gagcagctaa catcaacata cgtgcttctg
    29881 cttgtgcttc ttcattcaat ggaacgtgga cagccatttg gtctccatcg aaatcggcat
    29941 tataagcttc acatacaagt ggatgcaaac ggattgcgcg tccttgtact aaaactggtt
    30001 cgaatgcttg gataccaagt cggtgaagcg taggtgcccg gttcagcaat actgggtgtt
    30061 ccttgattac gtcttctaaa acatcccata cttcgtcttc ttgacgttcg attttacgtt
    30121 tcgcattttt gatgtttgaa gctagttcgc gttgaacaag ctcacgcatc acgaatggtt
    30181 tgaataattc aatggccatt tcttttggaa gtccacattg gtacatcttc aagaaaggtc
    30241 caacaacgat aacggaacga cctgagtagt cgacacgttt acctagcaag ttttgacgga
    30301 aacgaccttg tttccctttc aacatatgag aaagagattt caatgggcgg ttacctggtc
    30361 cagtaactgg acgtccgcga cgaccgttat cgatcaatgc atctactgct tcttgcagca
    30421 tacgtttttc attttgaacg atgattccag gtgcattcaa gtctaacaaa cgtttcaaac
    30481 ggttattacg gttgattaca cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc
    30541 caccttctaa ttgaaccatt gggcgtaaat ctggtggaat aacagggata acgtccataa
    30601 ccatccagct tggttgattt cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga
    30661 ttgcacgtgt acgtttttgt ccttgtgcag atttcaattc ttctttcaat tcggctgctt
    30721 ctgcatctag atcaacatta tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt
    30781 ggaattcttg tccatattgt tcacgttttt cgcgatattc gcgttctgtc aacaattgtt
    30841 ttttctctaa attcgtattt cctggttcga tgactacata tgaagcaaaa taaatgattt
    30901 cttccaaagc gcgtgggctc atgtctaata caagacccat acgtgaagga atccctttaa
    30961 agtaccagat atgtgaaaca ggagctgcta attcaatatg tcccatgcgt tcacggcgaa
    31021 ctttcgaacg agtaacttcc actccgcatc gatcacaaac gattccttta taacggatac
    31081 gtttgtattt tccgcaggca cattcccagt cttttgtagg accaaaaatc cgctcacaga
    31141 acaacccttc gcgttcaggt tttaaggtac gatagttaat cgtctcaggt ttttttactt
    31201 caccgtaaga ccagcttctg attttttcgg gagaagccaa acctatttgc atactttcga
    31261 atttatttac atcgatcaaa aggggttccc tccatttcat ttaatggcaa accgcaagac
    31321 ggctgatctt cgaccgcctt gcagctcata gaccggatta gtctaaatta tcttctgcgg
    31381 tttcaaagtt ttggatgaca tcgtcttttt catcttccac ttgttcagca gctttctttt
    31441 ctaattcttt ggcagtttgc tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat
    31501 cgtcgtcttc atcgtccatg tcgcgaagtt cgatctctgt ttcttcgata tccaagacac
    31561 gcatgtccag acctagtgat tgcaattctt tcaccaatac gcggaatgat tccggtacac
    31621 ctggttttgg aattggttca cccttgacga ttgcttcgta tgttttcaca cgtccgacta
    31681 cgtcatctga tttgtatgtc aagatttctt gtaaagtata agccgcaccg taagcttcca
    31741 gtgcccatac ttccatttca ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg
    31801 gttgttgtgt aaccaatgag taaggtccga ttgaacgtgc atgcaatttg tcatcgacca
    31861 tgtgagcaag tttgatcatg tacatcacac cgacagacac acgtccatca aatggttcac
    31921 ctgtacgtcc gtcgtacaga acagttttcg catcgctggc cataccggct tcacgaactg
    31981 tttcccatac gtcttcatca cttgcaccat caaatactgg cgttgctacg tggataccta
    32041 actgacgtgc agccataccc aagtgtaatt ccaatacttg cccgatgttc atacgtgaag
    32101 gcacccctaa aggattcagc atgatatcga ttggtgttcc atcaggtagg aatggcatat
    32161 cttcttccgg cataatacgg gatacaaccc ctttatttcc gtgacgaccg gccattttat
    32221 ccccttcatg gattttacgt ttttgaacga tataaacacg aactaacatg tttacgcctg
    32281 gtgacaattc atctccagct tcacgagtaa agattttcac atcgtgaacg ataccgccgc
    32341 cgccatgtgg tacacgtaat gatgtatcgc ggacttcacg agctttttcg ccaaagatcg
    32401 catgcaatag acgttcttct gcagataatt ctgttacccc ttttggcgtg actttcccta
    32461 caagcaaatc gccatcttgg acttctgcac caatacggat gatacccatt tcgtctaaat
    32521 cttttaatgc gtcttccccg acattaggga tttcgcgtgt gatttcttca ggtcctaatt
    32581 ttgtatcgcg ggcttctgat tcatattctt caatatggat agaagtgtat acgtcatctt
    32641 ttaccaaacg acggctcatg atgatcgcat cttcatagtt gtacccttcc caagtcatga
    32701 aaccaactaa aacgttttgg cctagagcca tttcgccttg ttccatagat ggtccgtctg
    32761 ccagagtatc ccctttttcg actttctcac ccaaatgaac gattggacgt tggttgtagc
    32821 ttgttccgga gtttgaacga cggaattttg ttacatcgta tttatctaat gcaccgttgt
    32881 cgcggcgtac gcggatttca gaagcatcaa cgtattcaac gacaccgtca tgtttacata
    32941 acaaagcagc tcctgagtca tgcgctgatt tgtattccat accagtacca acccatggtg
    33001 cttgaggatt gatcaacggc actgcttgac gctgcatgtt ggcacccatc aaggcacggt
    33061 tggagtcatc gttttccaag aaaggaatac atgctgtcgc aacggcaact acttgtttag
    33121 gtgatacgtc catgtagtct actttatcaa tagatacttc taagttttca ctttgagcac
    33181 gagccataac aacgtcttga gcaaatgttc catcttcatt caatgggctg tttgcttggg
    33241 ctacgatgta atggtcttcg atatcagctg tcaagtagtc gatttggtca gtaacacgac
    33301 ctgtttcacg gtctacacga cgataaggtg tttcgataaa tccgaattta tttactttcg
    33361 cataactgga caaactattg atcaacccga tgtttgggcc ttcaggagtt tcgatcggac
    33421 acatacggcc atagtgggag tagtgaacgt cacgaacttc ataaccggcg cggtcacgag
    33481 tcaatccgcc aggtcctaag gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt
    33541 ttgtttgatc catgaactgt gacaactgag aagaaccaaa gaattctttg atacttgcca
    33601 caactggacg gatattgatc aattgttgag gtgtcaatgt ttcagtatct tggatcgaca
    33661 ttctttcacg aaccacacgt tccatacgtg ctagcccgat acggaattgg ttttgtaaaa
    33721 gttcaccgac tgaacggata cgacgatttc ctaagtggtc gatatcatcg acattgccga
    33781 taccttccat caagttcaag aaatagctca ttgaagcaat gatgtcggct ggacgaaccg
    33841 tttttacagc tgcttctgga tagccgtttc cgatcacgtt tacttcacgt tcagggtctc
    33901 ttggtgaaaa tactttgatg acttggacag tcattggatc tgtgaccaca ccgtcttctg
    33961 atggatagta agtcactgaa tttaggccat tatcaatgaa aggagctagt gtttccatga
    34021 cttggtgagt cagaactgtt cctttttcaa cgatgatctc gcctgtttct ggatcaacta
    34081 atgtttcagc taatgtcaga ttcaatagac gtgttttcaa atctaatttt ttgtttactt
    34141 tgtaacgacc aacgtttgct aagtcgtaac gttttggatc gaagaaacga gcattcagca
    34201 agttacgtga gctgtctgct gtttttggtt cgcctggacg caaacgttcg taaacgtctt
    34261 tcaaaccttc ttctgttctt gagtcgcttg cgtttttatg caagtctttt tcgactgtat
    34321 tgcgtaatgt ttcgctgtcg ccgaagattt caaagatcgt atcatcggaa ccaaatccta
    34381 acgcacgaac taatacggtt aaaggaatct tacgtgtacg gtcgatacgt acataagaga
    34441 tgtcttttgc atcagtttcc atttctagcc aagctccacg gttagggata acagtagaac
    34501 cgaatccttc ttttccgttt ttgtcgactt ttccatggaa atagacacct ggtgaacgga
    34561 ctaactgaga aacgattact cgctctgccc cgttgatgat gaatgttcct tgttctgtca
    34621 ttaatgggaa atcaccgaag aatacttctt gtgctttgat ttcgccagtc tcgcggtttg
    34681 tcaaacgcaa tgttacatgt aatggtgcag agtagtttgc atcatgcgca cgagcttctg
    34741 ctactgtata ctttggttct ttcaattcat aatctacaaa ttccaaagat agattgccat
    34801 tgaagtcatc gattggcaaa atgtcttcaa acatttctct aagtccctca tctaagaacc
    34861 attggtaaga gtctgtttga atttcgatca aatttggtaa ttccaacact tcactgattc
    34921 ttgcgaaact tctacgttcg cgatgttttc cgtattttac tacgtgtcca gccaagctct
    34981 tcacccctct aaaatgtcat tcaaaaagac tacccgatca attgttacaa taatatttca
    35041 aatattattt ttaggtaaca acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa
    35101 aatagaacat tctcttgaaa ccttacttcg tgagcttcta cttttgtttc cttatattaa
    35161 agccgaaacg gacaatattt cgtttctgcc ttttcttcat cagatagttt ttgcaacttc
    35221 atatactaca cttattttct ataaaagtca acacttcctg gcgtttcttc tgctttttcg
    35281 caacattttc ttaatgtttt caattttatg ttttcaatta tctgacaatt cttaattgac
    35341 agaccttttt atctcggtta tactttagaa aattcatttt gtggaggaac ttctatgcga
    35401 accaacatca accaaatcgc accaacacaa ctgaataact tttactttag ctactttaga
    35461 taggtttgta ttcatcatga tggatacaaa cgttaaacga cagtttgtat ctatgatggc
    35521 taaagaactt tgtattgcag caaaaagcca cgtagacaaa atgctaagtg gctgagcagt
    35581 gaaaatacaa agtaaaagta tcaagcattc tactttttca atttattttc cgacttttca
    35641 atccatttgg acattttgtc tgaatggatt ttttgtttcc caaaattaag gaggaaaaag
    35701 aaatgaaaaa aaatataatc atcggattag gcgctgccct gctactagct ttaggaggtt
    35761 gcgcacaaaa agacaattcc gcagaggcat cagatggaca aaaaacaatt ggcgtattgc
    35821 aacttgtaca acataactcg ttagatgctg cctataaagg attcaaagaa ggattagcag
    35881 aagaagggta taaagaaggc gataatctaa caatcgatta tcaaaatgcc caaaacaatc
    35941 aagacaacct gaaaagtatg agtgagaaac tcgtcaaaga agatcctgat ttgctacttg
    36001 gaatcgccac tccagcagca caatctttat taaatgaaac aaccactatc ccgatgactg
    36061 tcacagcggt cacagacttg gaacaagccc atttagtcac atccaatgaa aaacctggcg
    36121 gcaatgtgac tggcacgaca gatatggtgc caatcgaaaa acaaatcggc ttactgttaa
    36181 atatcgtgcc agacgccaaa acaatcggca tcatgtataa caacggtgaa gcaaactcta
    36241 agatccaagc tgatttggca gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac
    36301 tgacagcaaa ttccacaaat gatgtccaac aagtgacgaa aagtctagct aaagatgtag
    36361 atggaatcta tattcctact gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag
    36421 tcgcaaaaga aacaaaaaca cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg
    36481 gtctagcaac agtaggaatc gactacgaaa gcttgggcaa acaaacaggg atcatggcag
    36541 caaaaatctt agacggcaag aatccaagtg aacttcctgt cgaaacagcg aatgatttgg
    36601 aactgtatgt caataaagac atggccaaag cattaggcat caatccagac agcatcaaag
    36661 aagctgaata aaacacagga ggattctcat gacaaatttg ctctctcttt tcttatcatc
    36721 tgcatcgcaa ggggtcttat gggccctact tgcgattggt gtatacgtca ctttccgtat
    36781 cttggacatt gccgacttga ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc
    36841 agtaagtatt acaaatggct atcatccatt gacagcttgt cttctaggct ttttaggggg
    36901 tgcggcagca gggcttgttt ccggactgct tcacacaaaa ttgaaaattc ccgcccttct
    36961 tgcagggatc atcaccatga ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc
    37021 gaacatctct ttattgggtc agccaacgat ctttcattgg gtcgagtcat tcggcataac
    37081 aaaagacaac gctgtactga tcgtcggtct gctttttgct ttgatcgttg tatgtttact
    37141 tgttttgttc ttacgtacag aaacaggact tgctattcgt gcaactggcg ataatctggc
    37201 aatgagcgaa gcaaacggga tcaacacaga taatatgaag atcatcggtt atatgatttc
    37261 taatggatgc attgctcttt ctggctcctt acttgcgcaa aacaacggct ttgctgattt
    37321 aaattctggt attggaacga tcgtaattgg acttgcttct attatcatcg cagaagtttt
    37381 gttacgtaac cagccactat ttctccgtct cttgactgtc gttttaggtg cggtcattta
    37441 tcgttttatt ttagcgttag tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc
    37501 ttctgctttc gtgctagtga tctgtctttc tttcccgcag atccagcaaa aattccattt
    37561 acctcaaaaa aagaaaggcg gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc
    37621 catcatacct ttgaaaaagg aacgatcaac gaaaatcatg tcctgaaagg cattaatctt
    37681 tccttagatc ccggcgactt cgtgacgatc atcggtggaa atggggctgg taaatctact
    37741 ttgctgaaca gcatcgccgg tacattttct gtcgatcagg gtcaaatttt attgaatgga
    37801 aaaaatatca cgaaaaaatc tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa
    37861 gatcctaaac tagggacagc cgttcgttta acggtagaag aaaatcttgc tcttgctatg
    37921 aaacgaggaa aaaaacgcgg cttcttccgt ggtgtcaaac cacaagaccg aacatttttc
    37981 aaagaccagc tggttcggct aaatttgggg ttggaaaatc gtttatcctc agaaatcggt
    38041 ttgctgtctg gaggacaacg tcaagctatt acgttattga tggccacatt acagcgtcca
    38101 gaactgatct tattagatga acacactgca gcgcttgacc ctgctacttc caaaacggtg
    38161 atggaattga cagaaaagct gatcactgag cagaatctca ctgcttttat ggtcactcat
    38221 gacatggaag acgcgatccg ctatgggagt cgactgatca tgctgcatca aggaaggatc
    38281 gtcgtagata tccagcaaga ggaaaaacag cagctcacag tgccccaatt attagcgttg
    38341 ttcaaacaaa acagcggcac ggcattaaaa gacgatcaag tacttttatc ttcataatca
    38401 gaaagaggat gggacaaaaa atgaacgccc accctcttct tttttctctt tccttctttc
    38461 gtttttgctt tatgtatcaa ccgttgtttc ttcgaaaact attttgttct ctccttcttc
    38521 ctgatagaat ggatgggaaa ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa
    38581 ttattgaaaa agacaccaga ttttctttcc ggagaaaagt tggcacagca actggaagtt
    38641 tcccggacaa gtatttggaa agccatcaaa gaattggaaa aggcaggcta tcgttttgag
    38701 cacgggccta cagggtatcg ttacctgccc tctgacgtac tagatgcaaa agaaatccag
    38761 caagagctac ggtcgtttat tcctgatttg aaagtacaaa cgatgttgac ttctgaatct
    38821 acgatgaaag atgccaaact ggcagcagct caaggagatt ctgtttcgaa gttatttgtt
    38881 gctgatacac aagaacaagc tcgtggacgt tttggacggc cttttttcgc tgaacctgga
    38941 cgaggaatct acatgagtct cttgctccat ccagacaaac ggtttgatga attgcctcaa
    39001 tatacgatca tcgcagccag tgcctgtgtg aaagccattg aatctgtaac cggtcgttct
    39061 gcttcgatca aatgggtcaa tgatatctac atggatggaa aaaaagtctg tgggatttta
    39121 tctgaagcag tcagtgatat ggaaagcggc aggatcagtc atatcatcat tgggatcggg
    39181 cttaattttt ctgttcctca aaatcatttt ccagctgatc tccaagaaaa agccatctct
    39241 gtctttcccg acggacaagc tagtgtcaca aggaatcagc taatcagcaa aatctggcaa
    39301 gaattgttca cttatctttc tcacccagaa aaagcgttgg acttctataa agaaaaatca
    39361 ttcgtattgg gaaaaaccgt gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca
    39421 aaagaaatcg gtgatcatgg cgaactgatc gtccaaacaa atgaacagaa gatgagtttg
    39481 tcttctggcg aaatcagtct cacatctatc cgtacgcaat aattatcaga tttcacaaaa
    39541 ggacacctga tctcatacag tgtccttttt tctgaattct cttcaaaaga ctctccttat
    39601 tctatcagct gacagtttct ctatcctcta gaaaaaggta ttctaaaaat atctataaag
    39661 catggtacac tttctttaaa tgaatcaatg caaaaaacaa gagagtcatc agtttacacg
    39721 atcaaaagag atagcaggcc gcttcctttt tcaatcttat agatagacca tgggtaacga
    39781 taaaaacaaa aaagaacttg cgacttattt tgccgtaagt tcttttttag ctagtgcctt
    39841 aatctcttaa tcccaacaaa cgtctcgcat agatcacctg accagagtgc atagcagcat
    39901 catcaataat agaaacgaga cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa
    39961 ttacttcatc tagctcggaa ggttccagcc ttcttaagta agaaatagca aaatcagcag
    40021 ctcctcgcaa gtatcctagc agatcttcta tttgattagc ttggatctgc tttgcttgtt
    40081 ccagactatg cttccaatct tgttcgttat ctgctatcgt catcggaaac cggctttccc
    40141 agtcttgact atgccaaatt ggagaagttc ctgctaaatc agcaatctgg aaatctaatt
    40201 ctcgagcggt atgccaagcc agccaagtca ttgatttgat ctgcggggct ctatcttcca
    40261 ctggaaaacg attggcctcc tcaatagaga cgccttcaaa caaacgttca aaccgatcgt
    40321 atgcccgatc cacactctcg catagcatat ctaaataatc catttatttt cctccttttt
    40381 atttatccta cttacttttt cctaattcta ggatacctac ttatatgtta ctacacaccc
    40441 ttatttttta cactttaaca gtcaccatca tcaatagttt ggagattgaa agaaaaagat
    40501 agtgtcgaac tatcaattga acaagaagca acaatatgaa cgtttcatta atattcttta
    40561 aataaacaat gatttttcaa acgaacagtt taagactagg agaaaaagag tataatagga
    40621 aaatgtttga aaggaaagga aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag
    40681 ttgctttgtt gttcaatatc ttttcgtatg gtgtggccgt ttcagcggaa acatacattc
    40741 ctaaagaccc tgggaatatt tataatgatc taactggtaa ctatggcttg ttaggaattg
    40801 ctagtcaatt ccacgtattt gctaaaaata agacaactgt gaatgcacat acagacggaa
    40861 atatagcaac acatgaactt gatgcaaaaa ataattttgg tacagagctt catgaaggtt
    40921 tattgaacca agaaataaat tatatacaaa aaactgattc tttcgctaat tcttcaggca
    40981 tacctacagg tagcacaaga atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac
    41041 ttcaagatgg tcatcctaca gtcaatggat ccaggcttga tcacttaact ccagatgatt
    41101 tttataaaga tcgtgctggg catgaatata ttgatttcga gcaggaattt gctaaattga
    41161 atgtagcagc aaatgatctt gcaacgatca ctccagctaa aacatatact gctgcagatt
    41221 ttccagacat gaacaatcgg acaatcgatt taactgattt atcagatacc gggtttttat
    41281 tggtaaatat cgatgcagaa gtgttaacga tgaatacgcc acttcaaatc attaatccaa
    41341 atgatcaagt agttgtattt aatgtcatca acagttcttc tgcattgaac gtacagtcac
    41401 caatcaaata caatgatcgt tcaaatcatg aaacagaaga tttttcggat gcgaatatct
    41461 cttggaattt tggaaatgag atgactgatt tgacaatcag tgctcctttt caaggaacaa
    41521 tccttgcgcc taatgctact atccgcatta ctcaaaacca agacggaacg attataggaa
    41581 caaatgtgat tttagatgca gctaccaatc gttgggatcc taacgaaata tttatcagcg
    41641 acaatggtac agatacgaca gacacaagcg acagtacaga tacatctacc acaagtgata
    41701 gctcagattc atcaacttca agcgacagta cagatactac ctctaccaca agtgatagca
    41761 cggatacatc agcttcaagc gacagtacag atactacctc taccacaagt gatagcacgg
    41821 acacatcagc ttcaagt
//
LOCUS       DACCKU010000016        41500 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.016, whole genome shotgun sequence.
ACCESSION   DACCKU010000016 DACCKU010000000
VERSION     DACCKU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 41500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.016"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1273..1938)
                     /locus_tag="IVN13_000779"
     CDS             complement(1273..1938)
                     /locus_tag="IVN13_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011290.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2064..4100
                     /locus_tag="IVN13_000780"
     CDS             2064..4100
                     /locus_tag="IVN13_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP7011291.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4104..4742
                     /locus_tag="IVN13_000781"
     CDS             4104..4742
                     /locus_tag="IVN13_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011292.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4770..5198
                     /locus_tag="IVN13_000782"
     CDS             4770..5198
                     /locus_tag="IVN13_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011293.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5655..6566
                     /locus_tag="IVN13_000783"
     CDS             5655..6566
                     /locus_tag="IVN13_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7011294.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6783..7220
                     /locus_tag="IVN13_000784"
     CDS             6783..7220
                     /locus_tag="IVN13_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011295.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7315..8199
                     /locus_tag="IVN13_000785"
     CDS             7315..8199
                     /locus_tag="IVN13_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7011296.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8423..11590
                     /locus_tag="IVN13_000786"
     CDS             8423..11590
                     /locus_tag="IVN13_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011297.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11610..12155
                     /locus_tag="IVN13_000787"
     CDS             11610..12155
                     /locus_tag="IVN13_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7011298.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12183..12638
                     /locus_tag="IVN13_000788"
     CDS             12183..12638
                     /locus_tag="IVN13_000788"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7011299.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12635..13393
                     /locus_tag="IVN13_000789"
     CDS             12635..13393
                     /locus_tag="IVN13_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7011300.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13894..15093
                     /locus_tag="IVN13_000790"
     CDS             13894..15093
                     /locus_tag="IVN13_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011301.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15115..15327
                     /locus_tag="IVN13_000791"
     CDS             15115..15327
                     /locus_tag="IVN13_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7011302.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15402..15878
                     /locus_tag="IVN13_000792"
     CDS             15402..15878
                     /locus_tag="IVN13_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7011303.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15875..17683
                     /locus_tag="IVN13_000793"
     CDS             15875..17683
                     /locus_tag="IVN13_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7011304.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17760..17975
                     /locus_tag="IVN13_000794"
     CDS             17760..17975
                     /locus_tag="IVN13_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7011305.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18067..18930
                     /locus_tag="IVN13_000795"
     CDS             18067..18930
                     /locus_tag="IVN13_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011306.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18961..19503
                     /locus_tag="IVN13_000796"
     CDS             18961..19503
                     /locus_tag="IVN13_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7011307.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19517..19939
                     /locus_tag="IVN13_000797"
     CDS             19517..19939
                     /locus_tag="IVN13_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7011308.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19869..22268
                     /locus_tag="IVN13_000798"
     CDS             19869..22268
                     /locus_tag="IVN13_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7011309.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22300..24291
                     /locus_tag="IVN13_000799"
     CDS             22300..24291
                     /locus_tag="IVN13_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7011310.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24314..24565
                     /locus_tag="IVN13_000800"
     CDS             24314..24565
                     /locus_tag="IVN13_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7011311.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24555..25784
                     /locus_tag="IVN13_000801"
     CDS             24555..25784
                     /locus_tag="IVN13_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7011312.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25781..27862
                     /gene="topB"
                     /locus_tag="IVN13_000802"
     CDS             25781..27862
                     /gene="topB"
                     /locus_tag="IVN13_000802"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7011313.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            28011..31931
                     /locus_tag="IVN13_000803"
     CDS             28011..31931
                     /locus_tag="IVN13_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7011314.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31932..32876
                     /locus_tag="IVN13_000804"
     CDS             31932..32876
                     /locus_tag="IVN13_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011315.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32880..33083
                     /locus_tag="IVN13_000805"
     CDS             32880..33083
                     /locus_tag="IVN13_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011316.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33383..33619)
                     /locus_tag="IVN13_000806"
     CDS             complement(33383..33619)
                     /locus_tag="IVN13_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011317.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33802..34173)
                     /locus_tag="IVN13_000807"
     CDS             complement(33802..34173)
                     /locus_tag="IVN13_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011318.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34170..35498)
                     /locus_tag="IVN13_000808"
     CDS             complement(34170..35498)
                     /locus_tag="IVN13_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7011319.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35459..35788)
                     /locus_tag="IVN13_000809"
     CDS             complement(35459..35788)
                     /locus_tag="IVN13_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7011320.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(36046..36417)
                     /locus_tag="IVN13_000810"
     CDS             complement(36046..36417)
                     /locus_tag="IVN13_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7011321.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36562..36747
                     /locus_tag="IVN13_000811"
     CDS             36562..36747
                     /locus_tag="IVN13_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7011322.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            37259..37477
                     /locus_tag="IVN13_000812"
     CDS             37259..37477
                     /locus_tag="IVN13_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011323.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            37690..38349
                     /locus_tag="IVN13_000813"
     CDS             37690..38349
                     /locus_tag="IVN13_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7011324.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38352..39695
                     /gene="vanS"
                     /locus_tag="IVN13_000814"
     CDS             38352..39695
                     /gene="vanS"
                     /locus_tag="IVN13_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7011325.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39866..40673
                     /locus_tag="IVN13_000815"
                     /pseudo
     CDS             39866..40673
                     /locus_tag="IVN13_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
     gene            40691..41500
                     /gene="vanW"
                     /locus_tag="IVN13_000816"
                     /pseudo
     CDS             40691..41500
                     /gene="vanW"
                     /locus_tag="IVN13_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11617 a         9541 c        10341 g        10001 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgaggacta cctgtattag tcctcttttt
       61 tgaccagatt ttgaccagtc tattttacat tctaatatat tcatatgagc taattttatg
      121 attaaatggt aagtgtttcc ttgtttgttt tagtttaaac tagtgtgtaa tttaaaaatc
      181 tatatggaag ggtctgtggg gtaatactta aataaaggct aggaacctga taacatcaag
      241 gttcctagcc tttttataat aaaatgaagc gaaaataatt tcttttaaag agccgagatt
      301 atcagctata taagagtgaa tttgatgcaa aaaacagagc cgaaacagtc ttggtgttcg
      361 tagctctgct tatatgtttc gaatttaaga caaagagttg gcaaaagatg ttgataagga
      421 agataagata actgtgttat ataaactagg taactaactg taacaagcta attttaagag
      481 attcttatta gaagacgttt aggaatataa ctaagaaaat gattatacag gtaattgtca
      541 tttcaataat gttttctaat tttcgcgtct ttgttttaag aaaaaggcca gaatcagttg
      601 gaaaaaagtt cgtacgatag tgaagagggg caaaatttcc tactaatatt cccactaaca
      661 agagataaaa cggccaggtt ggtgtgattc ctctataaaa aaatgcccat gctaccgaaa
      721 tcccatagat taacaactga acaattttta caatgaaaaa attgttctgc cattactcaa
      781 agtctcttcc tccttgtatc gtttatttgt ttcattttat cattatgata ggcgcttgaa
      841 caatccattt tgtattttat tgagtactct tggataaact accagcgatt atcatattta
      901 tttatgttac tgtaagccat catataaaag taaatgagca gatcatagaa atataaaaaa
      961 gaactaaaat tgatctaaat tatgtgcagc ttgactacgt accaatctat attacttact
     1021 agattggtcg ttaatctatt cggatcgaat gagcaaaata tttttgtgaa tgtccatcct
     1081 tgattatgat tccaaaaaga atcattggga ggctgtggcg taaatattat actataagcc
     1141 agaaatacct atcattctga acttttttat ttaggaactt tcatctcaag gctttttcaa
     1201 ttaatttgta cccctgtttt ttggataaga tcatgtgcat tgattttttc tatctctacg
     1261 ttcatctcct acttaatgcc tatctggctc ctgagggctt ctttttaagg cgtcgtcata
     1321 gtcttggctg aggatttctt ggtaatctga gggaatggct tcccaagtga gcaggcgaac
     1381 gttcatgacg tgttttcctt tgtgtttgat tacagcatgg tttttctctt tttctacctc
     1441 tccagcgtga aaagacagcc atgtaccatg gccaaagata tcattacata cccaacgttc
     1501 gatggaattg tccacgtatc ctacagggag tgtggtatag ccgatttgtg gtttcaaaaa
     1561 aggattgaat cgatcgatcg tttcattttt gtattgataa ttgataagaa aaagatagac
     1621 caaacaaaag acgagcagaa agaaagatag tgtctttttg agtgagaagg gttgttttga
     1681 tgaataagat ttcttgttaa caagaggttc atctggcaat gttggttcta ttgattcggt
     1741 agctgattca atagaaggaa aatcaatcgg cagacctaaa gtatgatgta aaagtgacag
     1801 catttcaagg tctggcattg tacgagaatt ttcccaacta gaaatcgttt tatcggaaac
     1861 accaattttt tcgcctaatt ctttttgcgt aagcttacaa tttctgcggt aagttcgtat
     1921 tttttctccg gtgttcatct tcttctcctt tcgattctac agtatataga tttattctac
     1981 atgacctata cacttgatag atacgttttt ttgatgaaaa acggtatgat tttatcaaag
     2041 aatcatttaa gtaagggaga gtggtgataa tcaaaaaaaa gttcacagtt ttactcacaa
     2101 tcatagtgac cggtatcatg agtggttttg ccttggttag tataaataaa caggtactct
     2161 atcggacaaa tgaatttttc catcaaacag atacttatac tccgattcga ttgccacaac
     2221 attttattgg gcaagaagaa tatgagcagc taatcatggt atttgaaaat gtctcatcag
     2281 aaactgagct ttcttatatg aaaaaaacag taaatgaatc caatgaagta gatacatctt
     2341 ggaacttcaa gatacagcca aagcttgaaa tgacattgta ttatcaaggg gatgtcaaat
     2401 cttctcacga gaaggtaccc tattctaata cctcctttcg cgtaacgtcc attgaagaat
     2461 cgatcaaaat aaaagatttt gaaggtgaat ttttcttgat gacgagagaa aaagaagcct
     2521 atgaacggtt catttcacta ttcactgatc gattcaatca agcatttcac actgattatg
     2581 ccactaatat ttttgaagac tttgaaaata gctattcgta ttcggttatt tcaaatacag
     2641 acgtatataa ttatcgagtg tatttaacta ttggattgat tcttttattc cttttgttat
     2701 catccttttt cttattgatg aataaagaaa tcagtttatt gttgaataat gacttttcat
     2761 tacatcaagt attgtggtgt ttattgggga aaaagaaaat cattattaca ttcataattg
     2821 caggaatcat tggtggtttt atttttatgt attataagga gcaatttctt tatttgttcc
     2881 agatgtacgt tttcttgttc agcggaatat atggtatctc tttgatttct cttttattag
     2941 ttgaaaaaat cgcttcgtta agagaagcgt tcaactatat ccaatacatt ctttttacat
     3001 ttggattgtt gttttctctt cttttgaccg ttgccaatgt cgaattagca actatcgttt
     3061 tttcttcttc acagttagct gtttcccatg aaccaccaaa agaaatcgct ggtgaggact
     3121 atcatgtatt ttatccagta accatcggga aaaaccacgt ggattttatt tattccaatc
     3181 gttttgcatc agcagcagat caggaattat atgaaacttt gaataaaaac ggaagtatat
     3241 tagtcaatgt atcagattac ttgaatgaag aaaatgaaca gtttggcaga gggataaaaa
     3301 taaatctgaa ttatcttaag aaatttcctt tgattgatgt ctctggaagg ttgatccaaa
     3361 taaccgaaaa agaaacgcac cctgttatat tgatccctga acgatataga catacagatt
     3421 taaaaaatga cctggcgcaa ataacagaat attatcaaga aatcagtgaa aaagaaccga
     3481 aatttctatt tataaaaaac aatcaaccca tttatacatt tattccttct atcccctgga
     3541 tcgaacatta tccggttgtt gaaatactga cattgaaaaa cagtatgtat ttggaaagaa
     3601 atattctcag cggagaaatc tatccaccgt taaagatcaa gataggctct ttgtcgaaag
     3661 aaaggttatt tgagctgatt gaagaaagtc agttacgtga taacttgcag ttatcatttc
     3721 cgtatactca gactgaagag atagctgtca aagtgttaag cagatcattt cattacttga
     3781 tacagatatt tttactcact ttcttttctt tcttcttgtt gagctatgtc atgttatgca
     3841 tatattttgt ctacaattgt cggaaaatcg ctatctttcg ttcaagtggt tattcgctct
     3901 ttgaaacgta taaagatttc ttcatgatga acctcatcaa atggggaacg acaagtgtaa
     3961 tcttcctttt tcttatagaa agagagccta aatatctttt taatattttt ttctttagct
     4021 tcattgattt tattctttca gttgttttta ttttgtctac agaaaagaaa agtcagttat
     4081 tgctgatgaa tggaggttaa caattggagc cgttgatagt gataaaaggt gcgaaaaaaa
     4141 tcattcgaaa caaaacgatt cttcatttgg atcgtttcac tgcggatgct ggggatcttg
     4201 tgactgttgt tggagaaagt ggagcaggta aaagtacgtt attgaatata ttaggattga
     4261 atgatcagtt cagttcagga caattctatt tgttcgatca agagacgaaa caattaactg
     4321 ggagagagaa gcgcttgata aaaagaaata ccatatcttt tctttttcaa gattttgggt
     4381 tgattgaata tgaaacaatc gattttaatt tagatattgg attgaagttt tcgaatttaa
     4441 atgtttcaga taagcgaaca gcaaagtatg aggcattgaa aaaagtaaat ataaacaaga
     4501 agctaagtac gcctatctac acattatctg gtggtgagaa gcaaagaatc gcctgtgcga
     4561 gagtcttatt gaagaatagc aaaatcattt tagccgatga accgactggg tcattggata
     4621 aaagaaataa ggataaagta gttgaaatct tatcgcaaca agcttcccaa ggtacgttag
     4681 ttatcgtagt cacgcatgat ccatatctga ccaaaatagg aaacaagaag atcatgcttt
     4741 aaaaatcaaa aaggaaagga tgtcgtttta tgaaaaaagc agttattatt tgtgcactag
     4801 ggataggttt tgttggagga aacgctatgt catctgaagt ggaagcaaat tcagttcacg
     4861 gtgaaatcta ttacaaatct ttaaagccag gagagattca ttttgatgct aaaggtaata
     4921 ttattaagtc aaatttacca gtagaccggg aaatttctag aacagcaatt aatcatagct
     4981 ctggaagatg ggtttattat agtgatacaa cttgtacgag aaaaacaggt cattctaacc
     5041 actatagtcg tgtctataat cgtcacggtg caaaagcgaa agttggaaac tctattcgat
     5101 cagcaaatgc aacaaaagga gtatgggcct atgcaagtgc gacaggaaat agaactgaca
     5161 cattctcatg ttggtacaat ccaaccggtt ggtattaagt atccaaaagt ttatataaaa
     5221 tagaaaaaac atataatgaa acagcgttaa aagttatttt tggtagtttt aatatattca
     5281 tttttagttg tataaaaata tatcataata aaaacaacaa aaaaatgtct ggaaaatcga
     5341 acataaaagt gtctgatttg aatatcccag cagcggaaga agaaaaacaa aatcgataac
     5401 atttgttcta agaattagtt acgttaaata cggaacgcaa gatagacttg aaaaacggta
     5461 acaaaagaag tagtatatta aggtgtgatg tgctacttga catttattct gctgagcgaa
     5521 ctttctggat tgtgtagcct tatatctatt caggacgtat cgagcagatt agtaaaggtg
     5581 ttcttgatga ttttcatctt gaacaccttt gtttttagca attcgcaaaa aacgaaaagt
     5641 aggagaattg ataaatgaaa aaaagatttt tagcacttgc catcgtacta ggcactggct
     5701 tattgtcagg ctgtacgaat gccggtgaaa aaacagccgt tagttataaa ggcggtacaa
     5761 tcagcgaaca agaagtaatg gatagcttga aaaaaatgca aggagcagat tccgctgtgc
     5821 aacagttgat cgtttatcaa gtatttgaag ataaatatgg agatgacgta tcaaccaaag
     5881 aaatcgattc tcaatatgat caaacaaaga aacaattggg tgattcattt gattcacaat
     5941 tgaaatctgc aggttacaca gaacaaacat tcaaagacag tatcaaacaa agtctagcct
     6001 ttcaagaagg tctaaagaaa catatcaaat tgaccgatga agatttaaaa acggcttggg
     6061 aatctttcca tccagaagtg gaagctcaaa ttatccaagt agcaagtgaa gatgatgcaa
     6121 aagatgtgaa gaaagctgct gataagggag acgatttctc taaattagcc aaagacaagt
     6181 caactgatac tacgacaaaa gaagatggcg gaaaagtgaa atttgattcc actactacaa
     6241 cggttccggc agaagtcaaa gaagcagcct ttaagttgaa agatggtcaa gtttctgatg
     6301 tgatcacttc taccaatgct tcaacttata ctacggaata ttatgtggtt aaaatggtga
     6361 aaaaccaaaa caaaggcaac gacatggata aatacaaaaa agaattgaaa gaaattgcaa
     6421 cagacacaaa attaagtgac agtaccttcc agaataaagt aattggcgaa gtattgaaag
     6481 acgctaatgt aaaaatcaaa gacaaagact ttgaaaatgt tttgagtaca ttcacttcag
     6541 attctagtac agcaagtagt aaataagcgg tataaataaa aaatatgaac tatttaaatc
     6601 aataaaactg ttcatctaaa tctttttatt agaactaaat caacagtagt ttaattacta
     6661 cttgatttag ttctttttat tttaaaaatt ttttttactt agaatccagc gaccctagat
     6721 atttttctgt ataatcttaa ataaagaaaa caatatcaaa aaatgcacgt agaagtagat
     6781 aaatggagtg gttttgcaaa tttcttttat ttctagaagt tattcacgta tacggatttg
     6841 tttttctcac aaataaaaag gcaggaagta caatggatca tttgtatata aaatatattc
     6901 atcaattttc cgttttggtg ttaggcacac taattatagc ggggattgtc gcatttatta
     6961 ttcaaggccg tgacatatta acagtacatc aagagttttt cagttatgca tttggtattc
     7021 ctctgatcgc tataaggtct gataacaaaa agtggaagaa acgctattac attctcttgc
     7081 tatttttgtg tttttatctg ccacctaaga tttttacttt attaggtata caatgcctat
     7141 cattttcttt tatttcctat atcattgttt taattttcct cagtttcata gtatggatgt
     7201 tttatcttgt caaaaaataa atgaatgttt catgaacagt acttcatcac agcctaattt
     7261 ttttctttat gctatcgtta tattaaagaa atcaatcaga agggaaagaa agtgatgaaa
     7321 aaagttttat tagtggtaaa ccccagttca ggcggtgaac aagcaaagga atttgagcaa
     7381 ttagcgatag caaaattgga atcggtgttt gatgaagtgg tcgtcttgca tacaaaaaaa
     7441 gcaggggatg caaaaaactt tactcgcgaa gcggctacgg aagggtatca tagtgtgttt
     7501 gtcatgggcg gagatggaac agtcaacgaa ggaatcagtg gaatcgctga acaagaacat
     7561 cggccaaatt tcggattttt cccattgggt acggtgaatg acttggctcg agcattaggg
     7621 ataccgctag aacctgagga agcgatcaat catttttcga ttgaatcagt caagccattg
     7681 gatattggaa aaataaatga cgactatttt atgaatgtcg tcgcaattgg ctctatccct
     7741 gaatcaatca atgatgtaga tgcggagaag aagacgaaat ttggaaaact agcttatttc
     7801 atgtcaggaa tcaaacagtt agcttccaca caatcctact cgtttcacgt agaagttgat
     7861 ggaaagaagg aagaaattga gagcagtacc ttgctgatag gactaactaa ctcagtcggc
     7921 ggttttgaaa cattacttcc aaatgcaaaa gtggatgatg gtaagctcca tttggtttat
     7981 ttaaaagatt cttctttgat cgatacgctt aaaacattgc cagacctatt aaaaggagta
     8041 gatgaatcta cgaaaaatct agcctacctt acttgtgagc aattagttgt gtcattagct
     8101 gatgaggaag aattgacgac gaatgttgat ggagatgaag gggataaatt acctgtgaca
     8161 atcaaagtat tgccctcaca tttacaggtt tattgttaaa atggaggttg tgaccgagga
     8221 gttggcggga gaacaccgct tgttcgggat aagggactgt gacaagactt ttgtcacagc
     8281 cttttttttg tacaaaaaag aaatttacac ctaatagaaa aatttttcca taaaaataca
     8341 tattttaaat atgaaagtac aattggaaat agattaaaac gataaaataa aattgtcaaa
     8401 acgtcagaga ggatggtgga gaatgaatca atgtgtaaaa aagctgattg cgtcgatttt
     8461 gtttgtcttc cctctcttga tggtgacatc cgtttttgcc gatgtagaga aaacaaacga
     8521 atcaacgcaa tcagagaatc aaagttttga caagactact ttttcagaag aatcgattac
     8581 ttctgaaact acagaatctc aaagtgaggc ttctactaat aaaaggcaag aagaaaataa
     8641 gacaagtgag acctcagaag tagctgaaca aacgtctgat agcagtgaag cggcactggc
     8701 aagagctgtt tcagcggatg ggtacagcga agctgccaca acagaagaac tggcgcagct
     8761 tctcgcagac gaatcagtaa ctaagatccg actgattcag cctttgactt tagacaggga
     8821 attagagatc aaacgagata tcgttattga ttttggtgga ttcgctcaca atttcggcac
     8881 acaccatatt tatatcaatg agatacctca gtcgatcgag ttccagaatt ttaaaggaac
     8941 agctgcacat ccaggtggat tgacaccaaa tatcgatgga aatgccatta tcattgctta
     9001 tatgaccgat ttttggggaa gcagatatca ttttacggga gaaataaaat ttaccgggac
     9061 acttgatttg tatgatgggt ccaaattagg actgatttac gctcctcgtg caactgtcac
     9121 attagatggt gtttccggtg tgttggatgt ccaaccagtg aaggaaggac tgaatgccgc
     9181 tccgggtaaa gcctattttt gtagaagcta tcaattgaat gtgatcaatg gtagtcaact
     9241 ttatggaccg tatctaggga aattctacgg ttttctagat tcaggagatg agactggtag
     9301 tggaaaaaca gcgccaggaa tccatatctt aagcggttca aaggtctctc ttgattatga
     9361 tcgggcagat ggcgttagcg atgaaggaga ggcagtcgat acgctacctg gtaatgtcat
     9421 gtttgaagtc agtggttcgg gaagcgaatt ttcggtaaat acaaagatca atattaccaa
     9481 tgacaacaat cgcggaatca tccaaatgag aggttcagga tcgaaagtca ctgtatcgaa
     9541 tggtgggaaa atcaccatca atacagcaac tacgggcgga tttcgcttgc aaggagattc
     9601 tagtcaaatc catgtatcaa gtggtggaca aatctcggtc aaaatggctg gagatgacga
     9661 tcaacccggg aacaatggga tgcgttttgt aggaaaaggc ctttctttga tcgtagaagg
     9721 agccggatca gttatcgaca tcgataagca gagtgggcgt tccagcgcag ttcgttttga
     9781 gaatggtacg caaaagctga cagtaaccaa tggaggctca ttgagggtaa gaaatacagg
     9841 tgatggcaaa tcttacgtga acagactcaa tcaaggaaat caagcgatcc aattttatga
     9901 cgcttctggt tttctagaaa gtccaggttc agctgatttt acagtcactg gagaaaaatc
     9961 aaatatcgat atacgcgcag attccggagc aactgtagat acaactggag atattgattt
    10021 aaattttacg gcaggtgaaa aaacttattt agtcatggtc gggaaaacag gggaagataa
    10081 gaccggcatc atcagtgggg atgtgttgaa tgtggactta gaatctccta cctattttga
    10141 tttccaaaac aaacggattg gaagcgagac caatggcata ggcggctggg tattccaagg
    10201 aaatggagat tcttctttaa agatcgccca ttcagaaatc gctttatggc gaaaaacagg
    10261 cgcaacattc gataactttg aaaaagaccc ggattatttt tctgatatga cagacttatc
    10321 gatcaatggg accgacttag gcaatttcgt atcaagccct agtgcaggtt taacaaatga
    10381 atttgctcag acaggtgccg gaatgaaaaa ttataatcga atcagtgcca ataatcagct
    10441 cccgatgatt gaatccttaa gagtgccaac gaatgcagat aaaaaaatct atgggcatgt
    10501 cgttgttccc gaaggtgtcg aggcaaagcc aagagatgct tggacaggcg aagtagaagt
    10561 gactttacgt attacttacg cagatacgac gaaatcatct gtagaaatta ctgctctcac
    10621 gcaaggacaa acagatgaaa atgaaggggc aggatataac ccatggggag aaggcgaaca
    10681 aggcggcttg ttcgaagttc aagtacccaa tgatgagaga ttaaaaacgg aggatcaagt
    10741 gcaagttgtt gctgctaaaa aagtaagagg tggccaagaa gtgacagaac tggactctgc
    10801 caaaacgaca gtcgatgtga ttcctcctga acctgctaat ttaagtacca cattgatcaa
    10861 tgcagcttct cgcacgatca caggtacagg tgaagcagga gccttggttt cattgaaaaa
    10921 aaatcaagct gttttgtcag aaacaactat tgatgataca ggggaatttg ttctttctat
    10981 cccagaaaat caactttttg caggagatgt attagaaatt gtattaaacg accaagcagg
    11041 agaagctact gctaagggtg tgcagaacaa gccaagtacc aacgatgaaa ttgggaatca
    11101 caatccaagt gatacgccag ttgtttatca tgatgccccg ccttttgcac ctgctacgaa
    11161 gatcgtagta gaaggtggtc tctcatttct tgccccttcc aaactcgatt ttggtcagat
    11221 caaagcaact ggtttgacac aagaagcatt tggccaaatg gatatacagg aaaaattatg
    11281 gatttatgat cagcgagaaa cgaaaaatcc gtggatctta acgataaaat tatctcagcc
    11341 tttcacaaat gatgcaggat tttctatgca ggatgtccta tatttcaaga aaggagagga
    11401 gtatcaatta gtgaatgaag aagaactgga aattgtcaga ggacaaaatt caacgaatgt
    11461 gcaagcagat tattcagaat atttaaataa cggtcgggcg tttaaacttg tcttaaccaa
    11521 agaaaatcag atagctggta attactcagc agagataaca tggacattag cagatgcgcc
    11581 gacagaataa agatcaagga ggatgattga tgaatcgaat atggaataaa agtcatcgaa
    11641 acaactgctc tttacttatg atccttctcc ttggattcat cctttggcag acacctgtcc
    11701 atgcctccga attcaatttt gcggtagatt ttgaattacc ggctaatcag attgataaga
    11761 acaaaacata tataaatgta aggatggagc ctgggaaaat acaggaaatc ccctacacgg
    11821 tctttaaccg aacagataaa tcagtaacag ttgagacatt gatccactcc gctacgacga
    11881 attcaaacgg tgtgatcgaa tatggagaat caaaaagtaa atttgatgaa acattaagtt
    11941 atgatatgaa aaacttagca aaaacagtgg gagaaataac tgttcctcct caaagtgcgg
    12001 tcactgagac actttcactg aaagcgccag aaaaagagct ggctggtgtg gtcgcaggag
    12061 ggatcacgtt tcaagaaaaa aaagagaacg tttctaacaa aagtacagtt ggtgtcgtca
    12121 atcagttttc tcaagtgaaa gcaatcttga tttgaaatag cgaaaaagag tcttccccag
    12181 atttgaaatt aaaaaaagct tatgcggatc aaagcgatgg aagaaatgtg attaaggcga
    12241 atcttcaaaa tccaaatgct tcttatatgt tcggcttgac tacatcggtt gaaatctaca
    12301 aaaaagaaac aaaagagcct tacctttcaa taaaaaaaga aaaaatagat gtagcaccca
    12361 atacaaattt tgatttactt gtcccattag aaggaaaaaa attagctccg ggacattatc
    12421 aagcggttgt gaagggtgct tggaaagaaa aacgctggaa tctccatacc gattttgaaa
    12481 taactgatga tgcagcggac caattgaatg aaaaggatat tgatttaaaa aatcagccag
    12541 aaacatttga ctggcgatgg ctgatacttg ttggagtcat tcttattggc ttatttgctg
    12601 gattatttat tttaaaagaa agaaggaaaa agaaatgaaa aaaacaattg cattaagtac
    12661 cgttctagct ggaagtctat tattatgtac acaaagtgtt tttgctgaga ataagagctc
    12721 agccactaca gaaggaacga tttctgtgac caaaccagat gctgcaaatc ctgaagacgt
    12781 tgttccagtt cctcctgttg atccaaatga tcctgatact ccttttacac ctgacgaacc
    12841 aaatcaggga acatccggat tactgacgat tgaccaagct ccagatcttg attttggaac
    12901 aatcaagtta ggtacagcaa aaacagtata tgctgcatta caaagcggaa cggacagtac
    12961 tggagcacaa acacaagtac agaatttcgt acaagtaacg gataaaagcg gaaattatgc
    13021 cggttggaaa ttatcagtca aacgaacaga atttattggt acaacatctg atacgaataa
    13081 actaactggt tcaaaggtga aattcaaaaa cgccgtgatt cgaaatagcg caactaatag
    13141 cagtggaatg ccatctaccg tttctggaac tggcgctgaa ggaatcgaaa tcccagcaga
    13201 tcaggcagta gatctagtaa ctgcaggagc aaatgaaggg attggcacat ggatttattc
    13261 attaggagag aatgtggaac aagggagaca atcagtggaa ttatttgttc ctgtatccaa
    13321 ttatgctgta gacaactacg tttcaacatt cacatggtcg ttaacagatg ctcctacaga
    13381 agaggaagcg taaaatcata tcaacgattt cagggagtgg agccgattca aggcccactc
    13441 cctgaaatct gctatgctta cgtctcagct cttattcatc aactcgaagc agttttttgg
    13501 aagtcatgga aacgttcctt tgtcttttca aagatctttc gaccaacgag taaggagtca
    13561 ccgttgaaaa attctaacac ataatgagca gaatcaattt tctgcaattg cgataagttg
    13621 atacagtaag attttaaaag taaaaatttt aggttatcgc tgaaagcccc ggaaatacgg
    13681 gcatttcgca ggatatgaga actcaattta ctaccaattt actactttcg gattgagccg
    13741 catatatgct gaaccatact gctaataccc gccaccccaa atacggcaca tcataaattg
    13801 aatacgacac cgtaaacgcg gcgttcctct ttcccgtcat gggagaacag gacgccgctt
    13861 ttttatgccc aaatagagaa aggaggcgac gccatgctat ttacttccgg ccctgaccgg
    13921 gcgtttgaac ggctgatgca ggaaaagccc ggtttcgatc actacgacgg cggcggggcg
    13981 gacgcgccgg aggattgcgg cacctgccga ttttaccgcc cggattggaa atatcaattc
    14041 tgccagtttg cagagtgtcc ctatcagccg ggcaagctga ccgactatgc agcggtcaaa
    14101 ttcacagtga aaggagcgaa caacgctatg gcagttttcc gtgtggaaaa gaacaaaggc
    14161 tatacggtga tgtcaaatca ccacctgcgg aataagaacc tgaccttaaa ggccaagggc
    14221 ctgctgtcgc agatgttgtc cttgccggag gattgggact ataccctaaa aggactgtcc
    14281 tatatcaacc gggaaagcat cgacgcgatc aggacagcgg tatgggagct tgaaaaagct
    14341 ggatatatcc ggcgtgagca gggccgtgac gcaaaaggca aaatggccga tatggtctat
    14401 accatctacg aacagccggt attggaaaac ccggtattgg aaaatccaac atcggataac
    14461 ccggtgttgg aaaatccgac atcggataat ccggcgtcgg aaaatccaac gcaattaaat
    14521 aaagaagtac aaagaactga cttatcaaaa aaagaaaaaa taattacaga tggacaaagt
    14581 acccattcca ttcctatccc atccccgacc cctgccccta tggagggcga ggcagcggct
    14641 acgccgccgg aacggaaaag gacaggaaag gatgaggctg tacggattta tcgggaaatc
    14701 attttggaga acatcgagta tgcctatctc attcaggaca ggagcattga ccgtgaacag
    14761 cttgacgaaa tcgtagacct gatgctggaa accgtctgca ccgcccgaaa gacaatccgt
    14821 gtcgccgggg acgactaccc tgccgagctg gtgaaatcca agtacatgaa gctgaccggc
    14881 gaacatatcc gctttgtcct tgactgtctg cgggagaaca caacgaaagt ccgcaatatc
    14941 aaacaatacc tgcgggctat gctgttcaac gcccccagca ccatttccaa ctactacacg
    15001 gcccttgtcg cgcacgacat ggcacagccc gattggggaa agcctaaatc cggcctgccg
    15061 gactattcct gcggcgaggg cgaaagcctg taacgagaaa aggaggacga ccctatgaag
    15121 cagggcgcat tgatttttga cgagcgaaca gaccgttacg acatccgctt tgatctggcc
    15181 gactattacg gcggcctgca ttgcggggag tgtttcgacg tgatgatcgg cggcaaatgg
    15241 aggcccaccc gcattgaaat ggccgaaaac tggtatcttg tcggcatccg ggccgacgac
    15301 ctttccggcc tgcgggtgcg gatttgacct atatctacct gcggcgtctg gtggttccgc
    15361 cttagacgcc gcttttgact ttctgacaag gagggatgcg attgcaggaa gaagttactc
    15421 aaaagaccat tgccctgtcc atgaaaacgg gcaagctcac ggctcaagtg ctgcaagcgg
    15481 cgctgaaaaa gtacctgcaa caccgggcca agggcaaaaa cacgctgcac cacggccagc
    15541 agagcctaaa gcagcttaaa aagcatgggg ctgatctctc caacattgag atcaccgagg
    15601 ccaacattgg agcgttcaag ccatgcgcca agaaatacgg cgtggatttt accttacgaa
    15661 aagataagac cacccagccg ccccactacg ttgtgatttt caaggccaag gatgcagata
    15721 accttgatct ggcgtttcgg gagtttaccg ccaagacgct ttccaaggag cagcggccct
    15781 ctatccgcaa ggttttgtcc gctgtgaagc aaaaggcggc ggcccagacc aagcagcggg
    15841 ccaaggagaa aatcaaggaa agggggctgg aactgtgaag cctgacgtaa agaaattgct
    15901 gatcctgaac ctcccgtatc tgatttttgt gtacctgttt gcgaaatgcg gggaggcgta
    15961 ccggctggcc gctggtgctg acctgtcggg gaagctcctg cattttgcgg atggttttgc
    16021 ggcggctttt gccaacccgc tgccaagcct gcacctgttt gacctgtgta tcggtatcgc
    16081 cggtgccgtg atcgtgcggc tggtggtgta ctgcaagagc aagaacgtga agaaataccg
    16141 caaaggcatg gagtatggca gcgcccgctg ggggacggcc aaagacatag cgccttacat
    16201 cgaccccaag tttgaaaata atatcctgct cacccaaacg gaacggctta ccatgaccgg
    16261 caggcccaaa gaccccaaga cagcgcggaa taaaaacgtg ctggtgatcg gtggcagcgg
    16321 ttcagggaaa acgagatttt tcgtgaaacc caacctgatg caatgctttc cgaccacgga
    16381 ctatccgact tcgtttgtgg tgacagatcc gaaaggcact ttggttctgg aaaccggcag
    16441 gatgttccag caggcgggct accgcattaa aatcctgaat accattaact tttccaagtc
    16501 catgaagtac aacccctttg tctacatcca ttcggaaaag gacgtgctaa agctggtaaa
    16561 taccctgatt tccaacacca agggcgaggg ggaaaagtca gcggaggatt tttgggtgaa
    16621 gtcggaacgg ttgttttatt cggccctgat cggctatatc tggtacgagg ccccagcgga
    16681 ggaaatgaac ttctcaacgc tgctggaaat gataaacgcc agtgaagccc gcgaggacga
    16741 cccggagttt caaagccccg tcgatctcat gtttgagcgt ttggagcaga aagaccccga
    16801 ccattttgcg gtgcgccagt acaagaaatt cctgctgtcc gcaggcaaga cccgttcttc
    16861 catcctgatt tcatgcggtg ccagacttgc gccctttgac atccgggagg tacgcgagct
    16921 gatggaggac gacgaaatgg agctggacac catcggggac gaaaagacgg tgctgtatct
    16981 cattatgagc gatacggaca cgacctttaa tttcattctg gctatgctcc aaagccagct
    17041 aatcaacctg ctttgtgacc gggctgatga tgtgtacggc gggcggctgc ctgtccatgt
    17101 acgcctgatt ttggatgaat ttgccaacat tggccaaata cccaatttcg ataaactcat
    17161 tgccaccatt cgaagccgtg aaatatcggc ctctattatt ttgcagagcc agtcgcagtt
    17221 aaaagcgatt tacaaggacg ccgccgagat catttcggat aactgcgata gtgtgctttt
    17281 ccttagtggg cgcggcaaaa atgccaagga gatttccgat gcgctgggga aagaaacgat
    17341 tgacagtttc aacaccagcg agaaccgggg aagccaaacg tcccacggat tgaactatca
    17401 aaaattagga aaggagttga tgtcccaaga cgaaattgcc gtgatggacg gcggcaagtg
    17461 tatcttgcag ctacggggtg tgcgcccgtt tttatcggag aaattcgata ttaccaagca
    17521 tccccggtac aaataccttg ccgacgccga caagaagaat acctttgacg tggacagatt
    17581 tttgactatc acgcgccgga aacggcagca agtggtaacg caggatgaag tattcgacct
    17641 gtatgaaatc gacctgtcgg acgaggacgc cgccgctgaa taaacagcgg cttttttcat
    17701 tccaacaaca aaatcaaaaa atcattatga gccggaaacc ggcagaaaga ggtaaattta
    17761 tggcattttt taattccgca gttactactt tgcagactat cgtgatcgga ctgggcggcg
    17821 ctctgtgcgt atggggcggc atcaatctgt tggagggata cgggcaggac aatccggggg
    17881 caaaatcgca gggtgtcaag cagcttgtcg ctggcggcgg cgtggccctg atcggtgtca
    17941 ctctggttcc cctgctgtcc gggctgctgg gctgatcctg gcctttgtcc ctgtccaaac
    18001 ccttaactaa tcgcctccgc gtcctcccgg ccagaccggg agggcgcggg aaaggaggtg
    18061 ctacccgtga ttagtgaaat cattgaagaa tggataaaag gcatcctcat ggacggtatc
    18121 acgggcaacc tgtccgggct gttcgatacg gtaaatgcca aagtcggaga aatcgccgcc
    18181 gacgtgggac gaaccccgca ggcatggaac agcggtattt tcaatatgct gcacagcctg
    18241 tcggaaaccg tgatcgtccc catagccggg gccatactcg cccttgtcat gtgctatgag
    18301 cttatccaga tgataacgga aaagaacaat atgcacgact ttgacagctc catgttcttc
    18361 cgctggatat tcaaatccgc gtttgcaatc ctgatcgtaa cgaacacatg gaacatcgtc
    18421 atgggcgtat tcgacgccac ccaaagcgtc gtcaatcaaa gcgccggtgt gattatcggg
    18481 cagacatcca ttgattttaa caccctgata ccagaccttg aaagccaatt ggaggctatg
    18541 agcgtgggcg ctcttttggg gctgtggttc caaacccttg ttgtgggcct caccatgaat
    18601 atcctttcca tctgcatttt ccttgtgacc tatggccgca tggtggaaat ctatgttgtc
    18661 acgtcgctgg ggcctatccc ccttgccaca atgggcaacg cggaatggcg cggcatgggc
    18721 caagggtatc tgaaatccct gctggcgctg ggcttccaag cctttttaat catggttgtg
    18781 gtgggtatct acgcggtgct gatacagaat atcggttccg cttccgacat atccggcgca
    18841 atctggggct gtatgggcta tacggtgctg ctgtgcttct gcctgttcaa gaccggcagt
    18901 atatcaaagg ccgtattcgg ggcgcattga tcccaaaatc caagcataca caggaggaac
    18961 ttgtttattt caacgtacaa catcatttac gctgacccgc cgtggcggta tgactgcaaa
    19021 aacgtacagg gcgcagcgga aaatcactat tccaccatga gcattgacga gctgtgcgcc
    19081 ctgccggtgg aacggctggc gtccaaagac tgcctgctgt ttttgtgggc cacattcccc
    19141 atgctgccgg aagctctgcg gttaatcaaa gcgtggggct tttcattcaa gaccgtggct
    19201 tttgtatggc tgaaacagaa ccgtaaaagc cttacatggt tttacgggct gggccgctgg
    19261 acaaggggaa acgcggaaat ctgcctgctg gcaacacacg gcaagccgaa gcgtcgttct
    19321 gcctctgtcc atcagtttat tatcagcccg attgaacaac acagcaaaaa gccggatgtg
    19381 acccgtgaaa aaatcgtaga gctggcgggc gatttgcccc gcgtggagct gtttgccaga
    19441 cagaaaaccc ccggctggga cgtgtggggg aacgaggtgg aatgtgacct tatcctgacg
    19501 tgaaaggagg tgccaaatgg cttatgtaac cgtccccaag gacttaaccc atgtgaaaag
    19561 caaggttgtg tttgggctta ccaaacggca actgatctgt ttttccgggg cgctggtgat
    19621 cggcgtcccc ttatattttc tcacccgtga ctatcttacc aacagcgcgg cggccctgct
    19681 catggtattt gccatgctgc cgggcttttt gcttgccatg tacgagcgtc acggccagcc
    19741 ccttgaagtg gtgattatgc agatcatcca gtgctgcttc atccagccaa aggagcgtcc
    19801 ctatcagacc gacaacacct acaccgccct tgtgcggcaa taccagatgg aacaggaggt
    19861 aaaggccatt gtccaaaaag gaaatcccaa gagaaaccga aaaaacaaag ctgacccgcg
    19921 cccagcgaaa agaaattgac gcggtaatcc gtaaatataa gggcgacggc aggccccaca
    19981 cggcgcagca gagcatcccc tacgaggtca tgtacccgga cggcgtgtgc cgggtatcgc
    20041 ccggcgtctt ttccaagtgc attgagtttg cggacatcag ctatcagctt gcccagccgg
    20101 acacgcagac ggctatcttt gaaaaactgt gcgacctgta taactatgtg gacgcctcca
    20161 ttcatatcca gttttctttc ctcaatcgga aagttgaccc ggtgcagtac gccaagagct
    20221 ttgagatcgc gccgcagggg gacgacttcg acgacatccg ggccgagtac accggcattc
    20281 tgcaaaagca gcttgcaaac ggcaacaacg gcatggtaaa gaccaaatac ctgacattca
    20341 ccatcgaggc cgaaagcgtg aaagcggcgc gagccagatt gaagcgcatc ggctttgacc
    20401 tgttaggcta tttcaagagc atgggggccg tagcccatgt gatggacggc tgggaacggc
    20461 tgaacctgct gcatggcgtg taccacccgg acggcgagat attcaatttt gactggaagt
    20521 ggctggcccc gtccggcctc tccaccaagg attttattgc cccgtcctcc ctgtgcttcg
    20581 gcaatgcaaa gacattcggc atgggcggca aatacggcgc ggtaagtttc ctgcaaatcc
    20641 tgtccccgga gctgtcggac gatatgctgg ccgacttcct caatacggaa agcggtgttt
    20701 tagtcaatct ccatgtgcag gccattgaac agaccaaggc gatcaagacc atcaagcgca
    20761 aaattaccga ccttgacgct atgaagattg cggagcagaa aaaggccgtc cgcagcggtt
    20821 acgatatgga cattcttccc agcgaccttg ccacctacgg cgaggacgcc aagaagctgc
    20881 tgacaaagtt gcagacccgc aatgaacggc tgttccagtt gacgttcctt gtcctcaacg
    20941 tggccgacac caagcaaaag ctgaataacg acgtgttcca agccgcaggc gtggcccaaa
    21001 agcataactg tccccttgtc cggctggact accagcagga acagggcctt gcaagcagcc
    21061 tgccgctggg cgtcaatcag atcaagatac agcgcagcct tacgacttcc agcgtggccg
    21121 tgttcgtccc ctttgtgacg caggagcttt ttcagggcgg cgcggccatg tattacggca
    21181 tcaacgccaa gtcccgcaac atgattatgc tggacaggaa gcaggcccgt tgccccaatg
    21241 ccttgaagct cggcaccccc ggcagcggca aatccatgag ctgtaaatcc gagatcgtca
    21301 gtgtgttctt aacgacgcct gacgatattt ttatttcaga cccggaggcc gaatattacc
    21361 cccttgtcaa acggctccac ggacaggtaa tccggctgtc gcctaccagc aaggactttg
    21421 tgaacccgct ggacatcaac ctgaattatt cggaggacga taacccgctg gccctgaaat
    21481 ccgactttgt gctgtcgttc tgtgagcttg tcatgggcgg caagaacggg ctggaagcca
    21541 ttgaaaagac cgtgatagac cgggctgtgc gcgtgatcta ccggccctat ctggcagacc
    21601 cccgcccgga gaatatgccg atcctgtccg acctccacaa agcgttgtta gatcagcatg
    21661 tcccggaggc tgaccgggtg gcgcaggccc ttgacctgta tgtgtccggc tccctgaacg
    21721 tatttaacca ccgtaccaac gtggacatcg gcaaccggct tgtgtccttt gacattaagg
    21781 agctgggcaa gcagctcaaa aagctgggga tgctcattgt ccaagaccag atatgggggc
    21841 gcgtcacggc caaccgaagc caaggaaagg ccacatggta ttttgccgac gagttccatt
    21901 tgcttttgaa agaggaacag acggcggcct attctgcgga gatttggaag cggtttagga
    21961 aatggggcgg cattccaaca ggtgccaccc agaacgtcaa agacctgctt tcctccccgg
    22021 aaattgaaaa tatccttgaa aacagcgact tcattacgct gctcaatcag gcgtccgggg
    22081 atagaaaaat ccttgcggaa cgcttgaatt tgtccaccga acagcagaag tacatcgaca
    22141 attccgaacc cggcgagggc cttttgatct ttgaaaacgt ggtgctgccc tttaccaacc
    22201 ccatcccgca caacacccag ctttacaaga ttatgacgac ccgcttaaac gaggtggcgg
    22261 gagtatgacc cggcgacgcc acaaaggagg tgaaccacca tgaaagagct gaaagccaag
    22321 gcgaaagtga cgcaggccat gacccgcgac gggctgacgc tggaaaatca ggccaccggc
    22381 gaggtggaga acatttccag ccgggaggcc gaacaggatt tgtccgcgcc ggggccgggc
    22441 ggcaccgccg ataagctgct ggaacgggcc gacgccatcc atgacggcca taaggccaag
    22501 aaagccgcga agcaggccgg ggctgtggcc gccgaggggg acgctgccct gcaccggcca
    22561 tccgcccggc tccaactgtc ggaggaagaa cgggccgacc cggatttaca gccctatatc
    22621 cgaaaatcgg acaaacgggc cgataagctg gataaggccc gcgccgccat ccccaagaag
    22681 cgcgtcccgg tcaaggaaac cgtctttgac gaggcaagcg gcaaagcaaa gaccgtcctg
    22741 cactttgaca aacaggacaa ggggcctccc tccctgaaac ccaatccggc aggccgtcct
    22801 gtttccgagg tttttctatt cggccacggg aaaatccatg aagtcgagca tgaaaacgtg
    22861 ggtgtggagg gcggccataa gggggaggaa cttgccgagc ggcaggccgg aaaagccatc
    22921 cgcagcacca tccggcacca caggctgaaa ccctactggg acgcggaaaa ggccgagcgc
    22981 aggctattgt ctgccaacgc ggagtatttc taccagaaat ctttgcggga taacccggag
    23041 atcgcacagg cggccagcaa ccccatttcc cggttctggc agaaacagca gatcaagcgc
    23101 cagtacgcca aggccgcccg gacagcgggc cagaccgcag gcgcggcggc acagggcgcg
    23161 gccaagacag ccggggcctc cacgaaagcg gccaaggccg cagccaaaga aagtgaacgg
    23221 gcggcctcct ttgtggcccg ccactggaaa ggggcgctgg ccgtccttgt gttggggctt
    23281 ttgatgctgc ttatcatcgg cggcttacag tcctgcaccg ccatgttcgg cagcgccgga
    23341 agcggcatca cggcgtcctc ttacttttcc gaggatagcg atatgttggc cgccgaggac
    23401 gcctacgcgg agctggaagc cgagctgcaa gactatctgg acaactacga ggccacccat
    23461 gactatgacg aataccattt tgaccttgac gagatcagcc atgaccccta tgtgctgatt
    23521 tccattctgt cggcgctcca tgacgggata tttaccatcg gggaggtaca gggtgatttg
    23581 tccatgctct ttgacaagca atatatcctg acagaaaacg tcgtggtaga aacccgttac
    23641 cgcaccgaaa ccgatacatg gacggacgcg gagggcaaca cccatacaga cacctaccaa
    23701 gtgccgtatg actattacat ctgctacgtc acgctggaaa acttcgacct gtcccatgtg
    23761 ccagtctata tcatggacga ggaaacgcta agtttctatt ccctgtatat gtccacgcta
    23821 ggaaaccggc ctgatctgtt ttccggcagc cagtacccca acgcctccac cctgacgccg
    23881 ccgacctact acgacgtacc cccggaggct ttggaggatg ccacctttgc cgccatgctc
    23941 aaagaggcgg aaaaatatat cggttatccc tatgtgtggg gcggcagctc tcccagcacc
    24001 agctttgatt gcagcggctt catttcgtgg gtaatcaatc atagtggatg gaacgtggga
    24061 cggctggggg cgcagggcct atataacatc tgcacccctg tttccagttc gcaggccaag
    24121 cccggcgacc ttgtattttt caagggaacc tacgacaccc ccggcgtgtc ccacgtcggc
    24181 ctgtatgtgg gcaacaatgt gatgctgcat tgcgggaacc cgatttccta cacgaacctt
    24241 acttcaaact actggcaatc ccatttttac agttacgggc gtttacctta acgccagaaa
    24301 ggagctttca attatggcaa ccaaacttga ccgcatcgaa aaggacatcc agaaaaccaa
    24361 gtctaagatc gcggagtttc agaaacagct tcgggagctg gaaacgcaga aaaccgaaca
    24421 ggagaatttg cagatcatcc agcttgtgcg cggcatgaac atgaagccgg aggaatttgc
    24481 cgcgttctta cgcagcggcg caatgcaggc cgcccctgcc gcaacccctt accatgaaca
    24541 ggaggacaat gcccatgaag aataagacca gacggatttc ccgtattctc cccgcacttc
    24601 tggccgtgat gctctgcgtc acggcctttc ccgttaccgc ccttgcaggc ggcaatgacc
    24661 cggcaccggc cccgctgccg gaggctacaa ccgaacccgc tacgggcggt ttggagcctg
    24721 aaacagacga aaccggcacc gccacggacg ggacagaaac cgaggccgaa aaagataccg
    24781 gccctctgac cggcaaggac atcaccgacc tgttttccgc gctgttcggt tccaaggtga
    24841 gcattgccgc cacggacgac ggcatccaga tcaccaccgg caccgacaaa aaagaacctg
    24901 aacagaccgg caccgtcacc accaacggcg gcaggctgaa tgtccgcacc ggcgcggggc
    24961 ttgacaagac cgcgttcacc cagcttccca atgggacgac ggttgaggta gtcggcacag
    25021 acggcgactg gattaaaatt ctcttgccgg agcgcatcgg ctatgtccat tccgactata
    25081 tgaccgtcag cgaaaaggaa gtagcggcta ccggcgaggg cggtttttcc ctctccattg
    25141 acccggagga aattgcttct ctgctggaac tgttcaatgg cggcggggcc agcgccgccc
    25201 tgtccccgga cgggaacctg tcactgattg acgacatcgg cagcgccgcg aaaaatggca
    25261 agcagtttat caccgtggaa accaaaaacg gcaatgtgtt ttatctgatt attgaccggg
    25321 acgacgaggg aaaagaaact gtccatttct taaatcaggt ggatgaagcc gatctgctga
    25381 cgctgatgga ggacggggaa accgtcaccc ccgctgctgt ctgctcctgt accacgaaat
    25441 gcaaggccgg ggctgtgaac acgaattgcc cggtatgtaa aaccaacctg accgagtgca
    25501 gcggcccgga gccgcaggaa ccccagccgg aggaacccga agccccggag gaagaaccca
    25561 agggcggcgc gggcggcctg atcgtgttcc tgctggtggc gctggcgggc ggcggtgcgg
    25621 ccctttacta tttcaagttc aaaaagccca aggctgaaac caccggcagc gacgatttgg
    25681 gcgagtatga ttttggggag gacgaggatt tggaggacga ggaacccgaa accgagtttg
    25741 acccggaagc tgattttgtg agtgaacagg aggacgagga atgacatata aacttgtaat
    25801 cgcggaaaaa cccagcgtgg cccgcagcat tgcgggcgtg atcggggcca cggagaaaca
    25861 cgacggctat ttggagggga acggctatct ggtgagctgg tgcatcggac atctggtgtc
    25921 ctttgccgac gccggacgct atgacgagcg tttcaaaaaa tggcgctatg aggatttgcc
    25981 cattctcccg gaaacatggc agtacatcgt ccccgacgac aagaaacagc agtttgacgt
    26041 tctctgttcg ctgatgcagc gccccgacgt gaccggcctt gtgtgcgcca ccgacgccgg
    26101 gcgcgagggg gaattgattt tccgtttcgt ctaccagatg gcgggatgcc aaaagccctt
    26161 tgagcgcctt tggatttcct ctatggagga cagtgctatt aaggacggct ttgcccacct
    26221 gaaaccgggc ggcgactatg atccgctgta tcaatcggcc ctgtgccggg ccaaggccga
    26281 ctggctgatc ggcattaacg ccacccggct tttttccgtc ctgtaccaca agaacctgac
    26341 tgtgggccgc gtccagacgc cgaccttaaa aatgctggtg gacagggacg ccaagattac
    26401 cgatttcaag aaagagaaat atcacatcgt ccatatcacg gcgggcggcg cggacgcggt
    26461 aagcagccgc ttttccgacg ctgccgaggc aaacaccgtc aaggcggctt gtgcaaaagc
    26521 gcaggccgtt tgcgtttccg tgacgcggga gaaaaagacg gaacagccgc ccaagctcta
    26581 tgaccttacc accttgcagc gggaggctaa ccggcttttc ggctataccg ccaagcagac
    26641 ccttgactat gcccaggcac tgtatctaaa gaagctgctg acctatccga gaacggatag
    26701 tcaatatctt accgacgata tgctgcccgc cgtggaaagc cttgtgtccg gcctgtggga
    26761 gctggtgccg tttgccaagg gcctgaacct ttccccgcag tttgaccgca tcctgaacag
    26821 caaaaaggtg tccgaccacc acgccattat ccccaccgct gaatttgtga agcagggctt
    26881 tggcactctg gccgagagtg aaaaaaagct gctgtccctg atctgctgca agctcttatg
    26941 tgccgtggcc gggccgcata tctatgaggc cgtcaccgcc acatttacct gtgccgggca
    27001 ggagtttacc gccaagggca agaccgtcct ttcccccggc tggaaagaga ttgaacgccg
    27061 gttccgttct tccttaaaga ccgacgccga cgaggacgcc gaggcggtgc gggagctgcc
    27121 ggagctttgc gagggccaga cctttgcaga cgtggccgcc tctgtcaccg agcatttcac
    27181 cacgccgccc aagccctaca ccgaagatac gcttttgtcc gctatggaac gggccggggc
    27241 cgaggatatg ccggaggatg ccgagcgcaa gggattaggc acaccggcca cccgcgccgc
    27301 cattctggaa aagctggtgc agatgggctt tgtccagaga aaaggcaagc agctcattcc
    27361 caccaaggac ggcatcaatc tggctgtggt gctgccggag gcgcttactt ctccccagct
    27421 tactgccgaa tgggaaaacc ggctgaccga gatcgccaag gggcaggccg atcctgacga
    27481 atttatggac ggcatcgaga cgcaggcgcg ggagctggtg cagacctatt cctgtatcag
    27541 tgaggaaaag cagaaattgt ttcaggccga gcgtgttcct atcgggacgt gtccccgctg
    27601 tggtgaggct gtctatgagg gcaagaaaaa ctactactgc ggaaaccggg cctgccagtt
    27661 cgtcatgtgg aagaatgacc gattttttga ggaacgcaag aaagcgttca ccccgaagat
    27721 cgccgctgcg ctgctcaaaa ccggcaaagt caaggtaaag ggcctctatt ccgtaaagac
    27781 cggcaaaacc tacgacggga cggtgctttt ggctgatacc gggggcaagt acgtcaacta
    27841 ccgcattgaa cacaggagct aaaggacagt aacacagcaa gcatagggag tgtggctaca
    27901 ctcccggtaa aatcccccgg ccgcgtccct gccgggccgg ggatttttgc ttgttgggga
    27961 agtccccaac cccctatttt ttgaaacaga acagagaaag gagtgaccgc gtgaacgaag
    28021 tattttctat ccaagtccat aaccggcagt ttttggaaca gggcaaacaa ggcgtctggc
    28081 tggatttgcc caccacaacc gaaacgctgc aagccgccct gcgggatgtt ggcattactg
    28141 ccgacaatcc gcaggatttt tttatcaacg ggtattcctg cccggaggac agacaccttg
    28201 ccattcccta tgacatggtg ctggccgccg acgtggacga gctgaatttc cttgccgccc
    28261 ggctgggcca gcttgacgcc gccgagatcg cagagctgaa cgccgccctg caaaatcccc
    28321 gtggcggctt tgagaatatc ggccagatca tcgactacac cgaaaatgtg gattactatg
    28381 tgcatcttcc cgacgttagg ggggatggac agttgggcga ctactacctg aaccgttccg
    28441 gcatggtgga tatgccggag gaatggaaag ccgggattga tacggcgcag tttggacgcc
    28501 acattgccca gcaggaacaa ggcgttttta cccagtacgg ctatctggtg agaagcggcg
    28561 acgaatggca gcgcgtccat gagggccagc cggtgccgga ggaataccgg gtgatgcact
    28621 acccccagcc ggaagctgac cgggaggaac cgcaggccca gcagccggag gcatccgaaa
    28681 gccccgacaa agcgccgccc ccggttatcc cgatcatgct ggacggcatg aacagcaaag
    28741 aacgcatgaa agtgattact gaccgtctgg aaaccggcgt acaggagctt tttgaaagta
    28801 gccgctatat ggcttactta tccgctatgt ccaagatgca cgattacagt ctgaacaata
    28861 cgctgctgat cgccatgcag ggcggttcga ttgtggcggg ctataacagg tggcaggatg
    28921 aatttgaccg ccatgtgaag cggggcgaga aaggcattaa gatactggcc ccggccccgt
    28981 tcaaggttaa gcagcagatg gagaaaatcg acccggccac ccagcagccc gtgatcggcg
    29041 cggacggcaa gcccgtgacc gaagaacagg aagtgacggt tccggccttt aaggtggtga
    29101 cagtctttga catcagccag accgagggca aggattttcc catgccctat atcagtgaac
    29161 ttaccggcga cgtgcagcag tatcaggatt tctttgccgc gctggaacgg acatcaccct
    29221 atcccataag cattgagccg atttcagacg gggccaaggg ccgctgcttc tacgcaaaac
    29281 agcgcattgc catcaacgag ggcatgagcg aattgcagac cctaaagacc gccatccatg
    29341 agattgccca tgccaagctc cacgccattg accgggaggc ccccgccgag gaacaggcgg
    29401 cccggccaga ccgcagcacc cgcgaggtac aggccgaaag cgtggcctat accgtctgcc
    29461 agtattacgg gctggacacg tcggactatt cttttggcta tgtggccgat tggagcagcg
    29521 gcagggagtt atccgagctg aaagcctccc ttgaaaccat ccgggccacg gccagcgaat
    29581 tgataaccga gatcgacggc cattttgccc agcttcagca ggagcgcgag gcccggcagg
    29641 aacaggaggc cgtcacggtt gacgttaccc cggaggcagc ggccccggcg caggccgagg
    29701 aagaacaggc cccggccctt gatccggcag ccgagccgct tgtgactgtg ctatggagcg
    29761 aaagccccca gctaaaggac ggccagcaaa tgccgttgca tgaggccgac gcgctgtttg
    29821 cccgtctgga tgcagcatat ccggcagagg caggctatga caaaaccgcg ttccgcattg
    29881 actgtatctt tcagggcgag ccggacagct acgaggggcg gcaggatttt ggagataggg
    29941 acggttccct gatcgaacat atccagagct accaccaatt ttacgcgcag gacgaaaact
    30001 ggaaaaagca tgtaatcgct acaaccggca tagagggctg ggagcaggac aaggcccatc
    30061 gggatatgct gttaggcgag tttgtcccct atatgaagct gcattgcagc cttgcccaaa
    30121 tggaacagac ggcgcagggc cgcttacagt ccggggaaac cctgacgccg gaggaaacgg
    30181 cctatttggg ggcgcttctc tcttatgtgc aggagtgccg ccccatgctc aatcagggcc
    30241 agtatcagtt gccggagccg ccgaaactgg ctgacttcga ccagagcttg caggactaca
    30301 aagcacaggt acaggccgag atcgcgcagg aggccgccgc agccggtatg accgtggagg
    30361 aatacgcggc ggctggctat gaagcacccc aacaggacac cttttccatc taccagcttc
    30421 gaggcgggga ggaaacccgc gactatcggt ttgagccata cgagcgttta caggccgcag
    30481 ggctggccgt ggacagtgcc aactatgacc ttgtttatac ggcaccgctg gataaaagca
    30541 cgtctttgga ggatatttac cgcacattca acatcgacca cccggcagat tttaggggac
    30601 acagcctttc cgtttccgac gtggtggtgc tgcaccagaa tggacaggac accgcccact
    30661 atgtagacag cttcggctat atggaagtgc cggagttttt gcgggaaaat cccctgcgta
    30721 ccgccgaact gtctacggag cagaacgaaa acatgattga cggcattatc aacaatacgc
    30781 cgcccgaacc gcagccggag gaacccgaac agctatcttt tgaggatgtg gcagttgctt
    30841 ccggccagac acaggaggaa cccccggagg ctgcgccccc gcaaacggct gtccgctatt
    30901 atcccatcaa tgagggagcc gcccgccgcg ccaaggaagc catgagcttt tctgactacc
    30961 ggcccggcag cgccacggcg gaataccggc actatgtcga tcaggccgtg gaaattgcgg
    31021 agcgccagaa aaagcgtgtg gagccagagt atcacgaaaa gatagacagc ctccttgaca
    31081 cctacgcccg caagctggcc gagaacatga ataagggcta tgagattacc gcccgcgtcc
    31141 cctctgtcct gattgccggg ccgtccaatt tcccggtacg ggcgaaagaa aagcagaacg
    31201 ccgccagtga ccgcaacatg gaggaatttc agtacattca gggcttgctt gacaaaatcc
    31261 gcagcaccgg catgggtggt atcagcgccg acgatccgca ggccgtttcc aagctggaaa
    31321 agaaactgga aaagctggaa gcgtcgcagg agttgatgaa ggccgtcaat gcctactacc
    31381 gcaagcatgg gacgctggac ggatgccccc atctgaccga aagaggcatt gaaaacctga
    31441 aagcggatat ggcgtcgggc tggcactatg agaaaaaacc gttccagtcc tggcagctct
    31501 ccaacaataa cgccgagata cgccggttaa agggccgcat tgaggaattg acccgccaaa
    31561 aggaggcggc ctatgtggga tgggagtttg acggcggcac tgtggagatc aaccgggtgg
    31621 ccaaccgcct gcaaatcttc tttgagggga agccggatgc tgctgtacgc gacgagctga
    31681 aaagcaacgg tttccgctgg tcgcccaagg ccggggcatg gcagcggcag ttaaacgaca
    31741 ccacaatccg cgtcgctgac cgtatcaagt gcatccagcc cctttccggc gaaaagccca
    31801 gcgccttaca gcttgcggcc cgcagggaga aagaaaagcc ctctatccgg gcgcagctca
    31861 acgcggccaa gaccgaacag gccaagcagc caccggcgcg ggaaaaatca aagggattgg
    31921 aggtagagta aatgcagaag tttgaaaatg tggacgttct ggccgcgctg gaacagatca
    31981 tgcggcagaa caccgcgttt tatcaaagtg actttggcat tgacaaagac attatcaggc
    32041 gggatgccgc cagcgatcag gccgcagaca agaccctgct ctggatgtcc cggccctccg
    32101 ggacgtactg tttccgggaa cgtgatgttt tcctgaaaga cacccgacag tacaacacat
    32161 ggaaatttta cggggagcag acccgtgaca agatactggc ctacgcggtg gagctgaccg
    32221 gcacacaggg cggcactatc cggggcaatc tctatgagct ggactatccg cagcatttcc
    32281 ggcacgtcat tgaaaccgcc cagccggtga gtgtgaaccg gctcttttat gagcatggca
    32341 cccgtgacat cccggagagc cagcgttttg acggaagccc tgaccgggta ttgggaaact
    32401 ttttgtacta tgaggcccag ccccatgacc cggctgtgct gcaggaggcg ctacggacgg
    32461 agcagcacag ccgggggcgg gccgtccccg gcgatttcaa agtccacgtc accgctttgc
    32521 atgacagccg gattgaaacc gaggcccggc gtattgtgga cagcctgaaa gatctgtccg
    32581 cgcctaacag ccccaataaa acgcatttca tggtggagat ctccccgcac tttgcggcga
    32641 ttgcttccac caaggataat gaccggctct ttgccatgct gccctataaa tccctgtgtt
    32701 ttaccgggat gaaaggcagg catggcgtgt atgccgtgat taaccgggac gagcgccggg
    32761 atgtgagtat tcgcaggccc cggccctcta tccgcaagca gctatccgac agcaaacagg
    32821 ccgccagccc aaaaaaggcg gcggccagaa ccaaaaagaa tgaattggag gtataaggaa
    32881 tgatgcactt taccgtggag gaagaaaacc tgatctgtat gtaccacaat gcagaccggc
    32941 gcagaacgat aaacaggctc accgccgtca tgccggttat ggatggggat atgcgggcgc
    33001 aggcccagca gaccctttcc aagctggaac gcatgacgga tgccgactat gacggccaga
    33061 agttccactt cacagacgaa taacgaacag cagacgccgg gcgcggcggc catgtggtcg
    33121 cttgcgtccg gcgtcttttc tttttgccta ttcctacgca tttagaaccc ggtattgtcg
    33181 gggataggat gaagtattcg cttgcccctg caaacatgcg ggagaagcct tgcagcgggc
    33241 ggttttcaat cccttaaatg cgtaggttgc agctataatt agacccctga aacagatata
    33301 gccgcgcagc gggtgtatct gtttcagggg tgtgcaggtg atagccgcaa agcggcgtaa
    33361 gagggtgcag ccctcttgcc gtctatctct gttccagcgg gaagctcggc aatgcttcca
    33421 gcagtttcag ggaaatgcgc tgccgtatat cttcgtcaat gactttcttc tttttgccgt
    33481 ttgcctgtct gatctccacc gtggaaagct cgttgatgta gtcagcataa tgggccaaca
    33541 ctttttccgt ggcccacctc tcaccggcaa cggcggcttt gatcgtttca tagtccagca
    33601 gcttttcttg tccgtccatc ctgatacccc cgtcagactg attttttcta tgataccata
    33661 ttcggaagca tggaaccaga gaaaacggcg cttttctgct ggtaaattct acgcatttag
    33721 aacagcactt cggcggggat aggataaaac acccacatac cccgctgacc atgccggaaa
    33781 agccttgaaa tataagggct tttacagctc taagtgcgta ggtttccagt atcttttccg
    33841 ctggcgctcg gcctctttgc ggcggcgcat ccgtttggag caggccgcgc agtatttggc
    33901 ccggttagac ttcggcgtga acgtggcccc gcactcacag cagcgtttca cttcgtcccg
    33961 gtctttggtg atctcggcat acagggccgc atccagcggg aggacagcgg ccttaaacca
    34021 gcggcacaga agcgaatagg aaatgctctg tacgcaaata cattcttcgc cgtcgtccag
    34081 caacaggcag ttgccgtcac aatagttgca gcactcatgc gtcagcttcc gggccttgcg
    34141 atattgaggg taattcattc gtggaatact catagctctt gcgtccattc ctttcccggc
    34201 gtcatacgca aaatgccgtc cacattctgc ttgatgatgc cgtattcctg catctgcttt
    34261 ttcgcttcgc cgtacttctc atacagccgt tccttttcct cggccaatcg cttgtactcg
    34321 gctttcaggg cgtacagatc ggggagcttt ttcacaccct gtccctttag ggcctttgcc
    34381 gcagcttcat acaaaatcaa cgtgctttca tgctcacgcc ggaattttgc cttatccgct
    34441 gcattgcggt attccatagc cacgggccag agctgcttgt aggtagaaat attctttatc
    34501 agcaccgcca tatccgcaag ccggtgttcc atagctttga gggtagcggc ggcctcgtcg
    34561 ctggcggcgg tgatctcggc cactttgctt tccaaatccg ggtaggtgtc tatcccatgt
    34621 tcagtcagga aattcaaagt ctttgcggcc tgcttcaaat tgtggagctt cgcccacctc
    34681 tcatagcctg ccgactgctg ggccttaatg ttgttttcca gatcaatccg caaagaaatc
    34741 cccttgcgct ctttgggcgc tctggtgata acgcggcctt tgatacgctc tttgatggct
    34801 tcctcggtgt aggcttcccc caatgtcttg cagcgggtaa agcgttcctg tccgggagcg
    34861 cggaaagaaa taaactttcc cggcttgatc tcatagccct gcgcctgcat gagccgcagg
    34921 aaatcatcaa aatcttttgc ctgcgggata gcggcgtcaa tggctgcttt gagcttcgct
    34981 ttccagctcg tccctttccg ctgggcgtcc cactcggcat agctcttgcc cttactctgg
    35041 ccgggcttca ccacggacaa tccgttttcc ttgcacagcc tgtcgctggt gcgccggata
    35101 taggcatagc tctgtttgtt ggaaatgtac ttgcgctgat ttaccatatc cacggcacag
    35161 aaaataatgt gattgtggac gtggccctta tcaatatggg tagtcagcac atagggatat
    35221 ttcccttgca gcacttcctc ggcaagctgc ttcccgatct catgggcctg ttccggcgtg
    35281 gcttcgccgg gggcaaagga ttgtatcagg tggtggccca gattattgcc cttttggtat
    35341 gcctgcgcca gcagcatttc aaattcaatg tccgctgttt cataagagca ggcaaaggag
    35401 gccaccagca gcttgccgtc agttttgtcc gggttctcga tgtagtcaag ggcttttttc
    35461 agcgttgact tgataggatg tatctttgta actgccatat ctgcgccagc gcccccttta
    35521 tatcctcgat gtcctgtgcg tacaccggcc ccataccgtt cagcctcttt gcaatctgat
    35581 tgacgttgac gccgatcttc tgtatctcgg cggtctgcgc ccggatgtcg ctggtgtcca
    35641 tgttgacgat atagccgtca atgagcatct tccgggcgta ggctgaaaag ttcttcgtat
    35701 gcagcagcgc cattttctgt tcaatcatag cccgttcctc cggcgtcaca gggacgcgca
    35761 gcacgatttt tcgctttctg tttgccatgc ttcaccgtcc tttccattca ggggtttggg
    35821 gatttcccca acaagcaaaa cggacgcggc ccggcaggga cggggcaggc cgtttttccg
    35881 ggagtggcta cactccctat gcttgctacg ttactgtttc tccactaaca agacgtttcc
    35941 aaagccccaa actacccgca aaatagccct ctggaaagaa aaaagtgcaa aaaaatagaa
    36001 gctacgctgt gcagctcccg ctaaaagtaa aacagaggat gccagttaca catcctccgt
    36061 ttcattcttc ttcggctttt caattagtat ggcgctgtca atcgcgccct cgatgatcgg
    36121 caggtattct tcggggcaga gtttcagctt gtggctgacc ctttgccgca gttcgctttc
    36181 ctcccgcatg atctccgggt taaagtagcg ttccacagga agcccgcagg ctttaataag
    36241 ctgtatgaca accggcagac tgggaatggc cccgtcgttt tcgatgttgg caagatagcg
    36301 ccagtcaata tttattttct ctgccattgc tttgcgcgaa acgcgctttg cttgccgggc
    36361 cgctttcaca tccgcgccga aagtttcaaa gccgggacaa tcttcaatat tcgccatatc
    36421 gcacctccca cttacattgt atatttcata ctctgcccgt ggaatgttgg tatatgcacc
    36481 tttcttaaag tttatatttc acattttgtt tcttttattc ttcgagcaaa agaattataa
    36541 tgtaccctgt ggaggtgtac tatggactac atgacgttga aagaggcagg cgagaaatgg
    36601 ggtgtgcccc cacgatggat aaactactat tgtgctgcgg gacgcatccc cggcgctgta
    36661 aagatggcga cgatctggct cattcccaaa actgcggaaa agccgattga caggcgtaca
    36721 aagaaaggaa aggcggttac tgaatagtat aatgaaaccg gcgcattttg gatatggttt
    36781 tgaagagaga gaaagcatgg tattttgtca cggaaaaact ataagatgat agatggcagg
    36841 ctgctccaga ccgataaaaa gtattctcat ctgaaacaga aagaaaagat ttacacatgg
    36901 atgtatgaag aagcgaaact ttatcatgac agaaatggaa aatgcccgaa acaaagacga
    36961 ggaagctgct gctgtatcag ccgtatatgg tagaattgaa aaggccggca tctggattct
    37021 ttatggtgaa gtctccagac attggataac tgctggtcta atgcgccttt gagtatgggg
    37081 tgattgattt taataccggg gaggtacggc tttttgagga aaatattctg ggatttacat
    37141 ttggcgatta ttacatttcc tgtaactgca aggacacgga tgatgaagat atgaggaata
    37201 tagacttgct gattttctac tgtcttgaac ggtattagga ctgaatctgc tgcgcggagt
    37261 gcggcaagag cgaccggagg gagtacacac aggccctgcc tgtgtgaaaa gccataccgc
    37321 aaggtgcatt cgcaaaaaag aggccgcgca ttttgctgag gcttggggtg tccccaacaa
    37381 gcaaaacgag aacgccgcag gcgggcaagt ggtggatcag aatatccgta aaggatattc
    37441 tgatccattg cttgccagta ttatcgggat gttttgacaa ccgctgtaat cgaaacatcc
    37501 caaacattaa gaaggtgtta attattatta tcttgattta attcgtttaa accaataata
    37561 tagatacata caccatgaaa agaagcgagt atcccaatga cggatgatta aaatccgaaa
    37621 agaaatcgct ttgaaactac agggaaacta cagactgtta tgttatcttc ttaaaatgga
    37681 gggattttta tgtcgatacg aattctactt gtcgaggatg atgatcatat ctgtaataca
    37741 gtaagggcgt ttctggctga ggcaggatat caggtggatg cctgcacaga tggaaatgag
    37801 gcatacacca agttttacga aaacacttat caactggtta ttcttgatat tatgctgccc
    37861 ggtatgaacg ggcatgaact tttgcgtgaa tttcgtgcga aaaatgatac tcccattctg
    37921 atgatgacag ccctgtcgga tgacgaaaac caaatccggg cgtttgatgc agaggcagac
    37981 gactatgtaa caaagccatt caagatgcag attttactaa agcgggtgga agccctgctg
    38041 cggcgcagcg gtgcgctggc aaaggaaatc cgtgtcggca ggctgacact tctgccggag
    38101 gatttcacgg tactttgtga cggtacagag ctgcccctga cacgaaaaga atttgaaatc
    38161 cttttgctgc tggtgcagaa caaaggcaga accttaacgc atgaaatcat tttgtcacgt
    38221 atatggggat atgactttga gagtgatggc agcacagtcc acactcatat caaaaatttg
    38281 cgggccaagc tgccggaaaa tatcatcaaa accattcgcg gtgtaggata ccgattggag
    38341 gaatcataat aatggaaaga aaagggattt tcattaaggt tttttcctat acgatcattg
    38401 tcctgctact gcttgtcggt gtaacagcaa cactgtttgc acagcaattt gtgtcttatt
    38461 tcagagtgat ggaattacag caaacagtaa aatcctatca gccattggtg gaactgattc
    38521 agaatagcga taggcttgat attcaagagg tggcagggct gtttcactac aataaccaat
    38581 cctttgagtt ttatattgaa gataaagagg gaagcgtact ctatgccaca ccaaatgcca
    38641 atacatcaaa tagttttaga cccgactttc tttatgtggt acatagagat gataatattt
    38701 cgattgttgc tcaaagcaag gcaggtgtgg gattgcttta tcaagggctg acaattcggg
    38761 gaattgttat gattgcgata atggttgtat tcagcctttt atgcgcgtat atctttgcgc
    38821 ggcaaatgac aacgccgatc aaagccttag cggacagtgc gaataaaatg gcaaacctga
    38881 aagatgtacc gccgccgctg gagcgaaagg atgagcttgg cgcactggct catgatatgc
    38941 attccatgta tgtcaggctg aaagaaacca tcgcaaggct ggaggatgaa atcgcaaggg
    39001 aacatgagtt ggaggaaaca cagcgatatt tctttgcggc agcttctcat gagctaaaaa
    39061 cgcccatcgc ggctacaagc gttctgttgg agggaatgct tgaaaatatc ggtgactaca
    39121 aagatcattc taagtatctg cgcgaatgca tcaaaatgat ggataggcag ggcaaaatca
    39181 tttccgaaat actggagctt gtcagcctga atgatgggag aatcgtaccc atagctgaac
    39241 cgttggacat agggcgcacg gttgccgagt tgctgcccga ttttcaaacc ttggcagagg
    39301 caaacaacca gcggttcgtc acagatattc cagccgggca aattgtcctg tccgatccga
    39361 ggctgctcca aaaggcacta tccaatgtca tattgaatgc agttcagaac acgccgcagg
    39421 gaggcgaggt acggatatgg agtgagcctg gtgctgaaaa atgccgcctt tttgttttga
    39481 acatgggcgt tcacattgat gatactgcgc ttccaaggct gttcacccca ttctatcgca
    39541 ttgatcaggc gcgaagcaga aaaagtgggc gaaacggttt aggacttgcc atcgtacaaa
    39601 aaacgctgga tgccatgagc ctccagtatg cgctggaaaa cacctcggat ggcgttttgt
    39661 tctggctgga tttaccgctc acatcaacat tgtaaatatt taaaacttaa atgattttga
    39721 ccgacaggta taaccctgcc ggtctttttt tgctgctaca ggaaaactac agactggcta
    39781 cagggaaagt acagatgcgc ttgccataat aacaatcgta ccagccacaa atcgtagttt
    39841 tattgcaaag gaggcattcg atcaaatgga aaaaagcaac tatcattcca atgcggatca
    39901 tcacaaacgg catatgaaac aatccgtgga aaagcgggct tttctatgtg cgttcattat
    39961 ttcgttcaca gtctgcacgc tgtttttggg ggtggagact ggcttccgta ttggaggcaa
    40021 cacagatacc gcccatccct gcaactcata caggcagcag cactgacgta gtggagaatt
    40081 tggaggaaaa cgctcttgcc accgccaaag aacagggaga tgaacaggaa tggagcctga
    40141 ttttagtgaa caggcagaac cccatccccg cacagtacga tgtggagctt gagcaactat
    40201 caaatggtga gcggatagat attcggattt ctccctatct tcaagatttg tttgatgccg
    40261 caagaactga tggagtttac ccgattgtcg catccggata ccgaacaaca gaaaaacagc
    40321 aagaaattat ggatgaaaaa attgccgaat ataaggcgaa aggctacacc tctgcacagg
    40381 ctaaagcgga agcagaaact tgggtggccg tgccgggaac gagcgagcat cagcttggtc
    40441 ttgctgtgga tatcaatgcg gacggaattc attcaacagg caacgaggtt tatagatggc
    40501 tggatgaaaa cagctatcgt tttggtttta ttcgtcgcta cccaccagac aagacagaga
    40561 taaccggtgt gagcaacgag ccgtggcatt accgatatgt tggcatcgaa gctgccacag
    40621 agatgtacaa ccaaggggtt tgccttgagg aatatttaaa accagaaaaa tgagaaaagg
    40681 atgttatgtt atggacagaa aaagattgac acagcgtttc ccgttcctgc ttccaatgag
    40741 acgggcgcag agaaaaatgt gcttttatgc gggaatgaga tttgacggct gtcgctatgc
    40801 gcagacgata ggggaaaaat cgctttccca tttgctcttt gaaacggatt gcgcattata
    40861 caaccacaat accggatttg acatgatata ccaggaaaac aaggtgttca acttaaagct
    40921 ggcagcaaag accttaaacg gcttattgat aagaccgggg gaaacctttt ctttctggtg
    40981 gctggtacgc catgcggaca aagatacccc ctataaagac ggccttacgg tgaccaatgg
    41041 taaactcacc accatgtcgg gcggcggtat gtgccagatg agcaatttac tattttggat
    41101 gttcctgcat acgccattga caattatcca gcgcagaggt cacgaagtaa aggagtttcc
    41161 agagccaaac agtgacgaga ttaaaggggt ggatgcaacc atttcagagg gctggattga
    41221 tttaaaagtg cgaaacgata ccgactgcac ctaccaaata tgagtgacct tagatgatga
    41281 gaaaatcatc ggtcaggtgt ccgccgacaa agagccccaa gcattataca aaattacaaa
    41341 tggcagtatc cagtatgtcc gtgaaagtgg cgggatttat gaatatgccc aggttaaacg
    41401 gatgcaagtt gccttaggta ccggggaaat aatagattgc aagctgcttt atacaaacaa
    41461 atgcaaaatc tgttatcccc tcccggaaag tgtggatatt
//
LOCUS       DACCKU010000017        39884 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.017, whole genome shotgun sequence.
ACCESSION   DACCKU010000017 DACCKU010000000
VERSION     DACCKU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 39884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.017"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(31..1263)
                     /locus_tag="IVN13_000817"
     CDS             complement(31..1263)
                     /locus_tag="IVN13_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7011326.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1517..2086
                     /locus_tag="IVN13_000818"
     CDS             1517..2086
                     /locus_tag="IVN13_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7011327.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2264..2704)
                     /locus_tag="IVN13_000819"
     CDS             complement(2264..2704)
                     /locus_tag="IVN13_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP7011328.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2862..3626
                     /gene="map"
                     /locus_tag="IVN13_000820"
     CDS             2862..3626
                     /gene="map"
                     /locus_tag="IVN13_000820"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP7011329.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3658..4581
                     /locus_tag="IVN13_000821"
     CDS             3658..4581
                     /locus_tag="IVN13_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP7011330.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            4657..5796
                     /locus_tag="IVN13_000822"
     CDS             4657..5796
                     /locus_tag="IVN13_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP7011331.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            5789..6589
                     /locus_tag="IVN13_000823"
     CDS             5789..6589
                     /locus_tag="IVN13_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7011332.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            6589..7416
                     /locus_tag="IVN13_000824"
     CDS             6589..7416
                     /locus_tag="IVN13_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7011333.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            7394..8128
                     /locus_tag="IVN13_000825"
     CDS             7394..8128
                     /locus_tag="IVN13_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7011334.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8228..9094
                     /gene="rfbA"
                     /locus_tag="IVN13_000826"
     CDS             8228..9094
                     /gene="rfbA"
                     /locus_tag="IVN13_000826"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP7011335.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9108..9680
                     /gene="rfbC"
                     /locus_tag="IVN13_000827"
     CDS             9108..9680
                     /gene="rfbC"
                     /locus_tag="IVN13_000827"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP7011336.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            9702..10730
                     /gene="rfbB"
                     /locus_tag="IVN13_000828"
     CDS             9702..10730
                     /gene="rfbB"
                     /locus_tag="IVN13_000828"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP7011337.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            10828..11679
                     /gene="rfbD"
                     /locus_tag="IVN13_000829"
     CDS             10828..11679
                     /gene="rfbD"
                     /locus_tag="IVN13_000829"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP7011338.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            11713..13746
                     /locus_tag="IVN13_000830"
     CDS             11713..13746
                     /locus_tag="IVN13_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011339.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            13790..15055
                     /locus_tag="IVN13_000831"
     CDS             13790..15055
                     /locus_tag="IVN13_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7011340.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNHNSYNSILNIYGLIGFGIAALLLILFIGRLTLKAD
                     LSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEEFYVME
                     EAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            15265..16071
                     /locus_tag="IVN13_000832"
     CDS             15265..16071
                     /locus_tag="IVN13_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011341.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16083..17303
                     /locus_tag="IVN13_000833"
     CDS             16083..17303
                     /locus_tag="IVN13_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011342.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            17293..18879
                     /locus_tag="IVN13_000834"
     CDS             17293..18879
                     /locus_tag="IVN13_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7011343.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            18918..21056
                     /locus_tag="IVN13_000835"
     CDS             18918..21056
                     /locus_tag="IVN13_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7011344.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(21424..22407)
                     /locus_tag="IVN13_000836"
     CDS             complement(21424..22407)
                     /locus_tag="IVN13_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP7011345.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            22785..24176
                     /locus_tag="IVN13_000837"
     CDS             22785..24176
                     /locus_tag="IVN13_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP7011346.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24192..25232
                     /locus_tag="IVN13_000838"
     CDS             24192..25232
                     /locus_tag="IVN13_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7011347.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            25251..26336
                     /locus_tag="IVN13_000839"
     CDS             25251..26336
                     /locus_tag="IVN13_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP7011348.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            26369..27331
                     /locus_tag="IVN13_000840"
     CDS             26369..27331
                     /locus_tag="IVN13_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP7011349.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            27324..28751
                     /locus_tag="IVN13_000841"
     CDS             27324..28751
                     /locus_tag="IVN13_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP7011350.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            28753..29823
                     /locus_tag="IVN13_000842"
     CDS             28753..29823
                     /locus_tag="IVN13_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011351.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            29810..31231
                     /locus_tag="IVN13_000843"
     CDS             29810..31231
                     /locus_tag="IVN13_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP7011352.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            31244..32251
                     /locus_tag="IVN13_000844"
     CDS             31244..32251
                     /locus_tag="IVN13_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7011353.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            32263..33267
                     /gene="neuB"
                     /locus_tag="IVN13_000845"
     CDS             32263..33267
                     /gene="neuB"
                     /locus_tag="IVN13_000845"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP7011354.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            33264..34412
                     /gene="neuC"
                     /locus_tag="IVN13_000846"
     CDS             33264..34412
                     /gene="neuC"
                     /locus_tag="IVN13_000846"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP7011355.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            34385..35062
                     /locus_tag="IVN13_000847"
     CDS             34385..35062
                     /locus_tag="IVN13_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7011356.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            35052..36194
                     /locus_tag="IVN13_000848"
     CDS             35052..36194
                     /locus_tag="IVN13_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP7011357.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            36207..37184
                     /locus_tag="IVN13_000849"
     CDS             36207..37184
                     /locus_tag="IVN13_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7011358.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            37184..38092
                     /locus_tag="IVN13_000850"
     CDS             37184..38092
                     /locus_tag="IVN13_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7011359.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            38093..38845
                     /locus_tag="IVN13_000851"
     CDS             38093..38845
                     /locus_tag="IVN13_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP7011360.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            38850..39797
                     /locus_tag="IVN13_000852"
     CDS             38850..39797
                     /locus_tag="IVN13_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP7011361.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        13311 a         6078 c         7788 g        12707 t
ORIGIN      
        1 attatttttt gcatgtgtgc gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg
       61 tactgtagag ccatcttcac ggattccgtc gatatccatc ttgtcagatc ctaccatgaa
      121 atctacgtgg gtttgacttc tattcaatcc agcttctgct aattcttcat cggacatttc
      181 tgtaccgcct ttcacactga aggcataagc agaacctagt gctaagtgat tcgatgcgtt
      241 ttcatcaaat aatgtattga aaaagatgat gccagattga gaaataggtg aagggtcagg
      301 tacaagtgcg acttctccta aacgacgcgc accttcatct gtatcaagca gttttgctaa
      361 gacatcttct ccttgttcgg cagaaaaatc aacaactttt ccatctttaa aagtaaattt
      421 catacccgag atgatggtgc cagcataact cagtggcttt gtacttgaaa tgtaaccatc
      481 tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt
      541 ttctccacga gcattatagc ttccagcgcc ttcccaaaga tggtttttag gcaatccgat
      601 aatgatatct gtaccgggag cagtatagtg aagggcagaa aattgttctc gattcaattc
      661 ttctgctttt tttgctaatt tttcatcatg cttcttccat gctaagacag ggtcttcttc
      721 atagacacga gtcgttttga aaatctgatc ccataatgcc tcaacttgtt tttcttctgg
      781 cagttctggg aagacttttg ctgcccattg tttaccagcc gcagccacga cagtccaact
      841 gactttattt gcttgagttg ccttgcgtaa attcattagt gctttgccat tcgctgattg
      901 ataagacgct actcgatggc tatctacacc agcaaatgca tcgggatcgg cagaaacaac
      961 gctgatcctg cttgctcctt tttcaagcca ttcatccgct tgatcgattt tagattgtgg
     1021 tacattctca atacgatccg ttgcagcatg taaaagaaat tctctttgga tctggtcgtc
     1081 agtccattga acgataacct cagcggctcc tagtttataa gcttcttgtg tgatcaatcg
     1141 agcaaggggt gcctgttcca cgctgatctg taaaacaact gtgtgtcctt tttctgtggc
     1201 tacgccagtt tctgcaatta agcgtgcata tttttttaga agttcattaa aatcagataa
     1261 cataataaaa attcctcctc accatttttt ctggtaacta ttagcatgat accatttaat
     1321 tatgattcaa acaatgaggc tttaaataaa aaaaggaggt tattttttta aggttaagtt
     1381 tcatgatata tgttactact tgctatttca gcttgtgaag aaaatcaaaa actaattttg
     1441 catatcaaaa aaacatgtta caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt
     1501 aaggagatta aaaaatatgg caagaaaaaa gacgattact agagaacaga ttttggcagc
     1561 tgcttatgaa gtagctgcca ccgatggatt tacacgattt actgcgagaa acatcgcgaa
     1621 caaaatgaat tgttccacgc aaccgattta tcttgagttt aaaaatatgg atgacttgag
     1681 acaagctctt tttgagcaga tctatgatca tttacaatat gaagtgttca ctgttgagca
     1741 tacagggaat acgattatcg atcttgcttt taattatatc cattttgcaa gaagagagaa
     1801 gaagttgtat cgttcactgt acttagaaga atatggtgga ggaaaagaga tgcagaattt
     1861 ctcttatact tactttgtga atacagtgaa acgcgatcca gcttatgtga atctttcaga
     1921 agagcagatc gaatctttgt ataatgggac atggatcata gctactggga tcgcagcttt
     1981 gatgtcttca agcatcatcc atccaactga tgaacagatt gaaaagatga tccaagacag
     2041 tatagatgct atcttgaaat tcaaagaacc aatcgaaatc gattgaatat actcaaaagt
     2101 tccacaaatc agtaggtaac aagagatgtg tcaaaataag gaaaagttcg tttttcttac
     2161 agcgtatgca tttttaactc ccgaagaact cttccatgaa tgcaatgaat aaaagagcca
     2221 tacagattga ataaatctgg atggctcttt tattgcaagt atattaaatt tttgcgacta
     2281 gttctttagc gaaagcttct aaattttcga tatcttcttc ttcagcattc agatccactt
     2341 taacattttc ggctcctttt gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca
     2401 ccgatttgca gaattcatcg taaaaagtat caccagatcc gcagacaccg taaatttttc
     2461 ccaatagatc caattcttgc aagtcttcat aaaagtctac gatctcatca ggcagatcgc
     2521 catcatcgta tgtataagta gctactacgc aaatatctgc ctcctcgaat tcatctgcat
     2581 ctacttgggt acattcgttg atttcgactt cgatatccag attttcaaat gcttctgcaa
     2641 caatatcggc gatttcttca gtgtttcctg tcatgctagc atatacgatt tttgctaaag
     2701 ccatgatgtg cctcctcgtt tataattatt taacaattat gaattatacc aaaacatcat
     2761 tgaaaagaaa ataaaaccta aagacacctg gggaatgtca tttggctaaa tgtatgccgc
     2821 tatgttaaaa tgagagggat ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag
     2881 cacgtgaaat tgaaatgatg gcagaatctg gagctttatt agcagatgta cataaaaatc
     2941 tgcgtgattt tataaaacca ggaatcacaa gttgggatat tgaggtattt gtcagaaact
     3001 atatcgagag tcatggcgga attgctgcgc aaattggctt tgaagggtat gaatatgcga
     3061 cttgtatcag tataaatgat gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa
     3121 acggtgattt ggtaaaagtc gatatgtgta tcgatttgaa aggcggcatt tctgattctt
     3181 gttgggccta tgtagttgga gaatctacac ctgagatcga ccgattgatg gaagtaacga
     3241 agaaagcttt gtacatcggt attgagcaag cacaagtagg gaatcggatt ggtgacatcg
     3301 gtcatgctat ccaaacttat gtagaaggcg aaaatctagc gatcgttcgt gattttattg
     3361 gtcatggcgt tggtcctacg attcatgaag aaccagcagt tcctcattac ggagaagctg
     3421 gaaaagggtt gcggttgaaa gaaggcatgg ttatcacgat cgaacctatg gttaataccg
     3481 gaacttggaa aatgaaaatg gatcctaacg gctggactgc gtatacaaga gacggcggac
     3541 tttcttgcca atatgaacat acattggcta ttactaaaga tggtccaaga attttgacat
     3601 cacaaggaga agaaggcact tattgatcta tccagtcaat aggtacggga gaggtacatg
     3661 aaagtttgga acaaaataaa acaaaatgaa aatctgatgc gctttattga gacgactcaa
     3721 aagcgcatca ctgattcaga aatgagtacg acctctgtcg tcgttgctta ttatttactc
     3781 ttgtctttat ttccattgat tattgctttt ggtaatattt taccgtttct ccacatcgat
     3841 caagagactg tactgaccta tttacgagaa gtgattcctg agaccgttta ccagtttatc
     3901 ggtcctgcaa tcaaagacct tttgacacaa agttcaggag gcttgttatc cgtttctgca
     3961 ttagcggctt tatggtctgc aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa
     4021 gcgtatggcg tagaaaatag aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc
     4081 attgtcttgt tcatgattgc tattagtggg gtaactatcg ttcttgggtt gggacagctg
     4141 attttggatg ctattcagcc aatcgtacaa atacccgtga actttatcga tcagttccaa
     4201 actttgaagt ggccaatcac attggtagct ttatttgtca tcatgttctt gatttattta
     4261 attgtcccta atgctcagtt gaagttgaag acgatcattc cgggcacgat cttcgctact
     4321 gtcggctgga tgctgttatc acaagtgttt agtatctatg ctaggtattt tgcaacgagg
     4381 atcagcggtt atcagatcat aggaagtttt attgtactca tgctttggct taattttgct
     4441 gctacgatca tcattttggg cggaatcatc aatgcagttg ttcaagaata cgtgacgggt
     4501 catcaaatca aaaaacgtcg agatccaagc cgaaaatggt tcaataaatt gaaaactaaa
     4561 ttttctcata aaaaaaagta gaaacaagag ttcttattgt aaatttatat cagtaattta
     4621 taataattat gactcttaat taaagtaggt gcccatatgc ttgtttcttt tagtatggtt
     4681 ataagatttt ttctgacaat tttattatca ctgatattaa cacctgtatt caaaatcatt
     4741 tcagtacaaa caggtatggt cgacaaacca aatgaaagaa gaataaataa agttccaatg
     4801 ccttcagcgg gcggactgcc tatctttatt tcatttgtta tatcgacttt attcttattc
     4861 cgtaaaatta tccctcaatc gtatattgta ccgatcttga ttgcttcttt agtaatcata
     4921 gtcacaggag tgctcgatga taaatatgag ctgacaccta aacaaaaaag tatagggatc
     4981 ttgattgctt ctcttattat ttattttgta gcagatatac gtattgattc cttcacgctt
     5041 ccttttattg gatatatcca actaggctgg cttagttttc ccgtaacgat tttttggatc
     5101 ttcggtatca cgaatgcggt caatctgatt gacggtttgg atgggctggc ggcagggatt
     5161 tcattgatcg gtttaattac gataggaatc attggatatt ttttcctcca tgcttctaca
     5221 gtgtatattc cagttgttat tttctgtctt gtagcaagta ttatcggttt ttttccttac
     5281 aatttttatc cggcaaagat ttatttaggc gatacaggag ctctattcct agggtttatg
     5341 atggccgtcc tttcactaca agggctgaag aacgtgactt ttgtttcatc gatttctttg
     5401 ctgatcgtca tgggagtgcc ggtaacagac acgttttttg ctatcattag gagaaaagca
     5461 aaccgtgttt ctttttctac agcagataaa aaacatcttc atcaccgact aatcagttta
     5521 ggctttactc ataaaggtgc tgtcctgacg atttatgcga tggctttgat gttttctttc
     5581 acagctatgg tcatgaatta tactggaaga atcggaacga tcatattgat tattgccatg
     5641 ttgtttgctg caattttatt atttgaattg attggtctga tcaatgagaa acatcagttt
     5701 attttaaaaa cattgcgatt tttagggaat aaagagtacc gtacgcaagt aaaacagaaa
     5761 tatttgaaga aatggaaccg tcatatagat gaataacaaa ttaaaccagc cgatccttat
     5821 tagtattgtg acggcaaaca gtgataaaat ttttcggaca ctagataatt ttatttcaac
     5881 tgtaaaagat gaaacagcag taaaaatccg aatttttgac aacaattcac ttcccgagta
     5941 caaaaaaaga ctaacagagt ataggaaata tccctacatc gatatctatt ttcatgatga
     6001 aaattccgga tttggttatg gacataatca caatctgata gctagcgatt ctcgctatgg
     6061 cattatttgt aacccagata ttttagtgga tgaagagaca gttacagcat tggtcttgtt
     6121 attgaaagaa catccggaat gtgcgatgtt agcacctaag attcttaatg aagatggaac
     6181 aactcagcac ctcatccgtg agcagctggc tgtttttgat tacgttttgc gctttgtgcc
     6241 tttcaaatgg gtcaaaaaaa agtttgacaa acgactggct gtgtttgaat gccgaggatt
     6301 gcctactgat gctaatagtt atgtacggat gatctcaggt agttttatgg tagctgatat
     6361 tgataaattt aaagagataa aggggtttga tgagcgtttc ttcatgtact ttgaagacaa
     6421 cgacttgtgt ttgagttttg aaaaagcagg ttacaagtta ttgtacacac ccttttatgc
     6481 agttatccat ttttacggga agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt
     6541 gacttctatg atgaaatttt tcaataagtg ggggtggcgt tttttctgat gaaaccagaa
     6601 atattagtag tcgttgtgct ttatcagcaa ctattcagtc aatctccagc ttatggacca
     6661 ttaagcaaag cacttagcga aaagcaaatt cagcttatta tttacgataa tagtcctcaa
     6721 aggcagcaaa atgagctgtt tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat
     6781 cctggtttag cggtggctta taattttgcg ttgtctcaag cagatgaagg aacacgttgt
     6841 ctcgtcactc tggatcagga tacgaggcta gtcgacgatt attttgaact attgcgtaaa
     6901 gtaaccttta cagatgaatg tgttgctgca gttccgatga ttttttctgg gagccgacaa
     6961 gtttctcccc tatattcaga tcgttatatc aatcgtacag ctcaagcagt agaagttgaa
     7021 ggcccaacag ctgaacgaat catggcaatc aattcaggtg ttgcctggtc tgtcaaattt
     7081 ctaaaaaaga ttggcgggtt taatcctgct ttttctttag attttttgga tcattggctg
     7141 ttttggaaag tggatcagtt ggaaaaaact gtggagatcc ttcctgtacg aatggaacat
     7201 gatctttcgg ttttagacta tgaaaaagtt tcaagtaatc gataccgttc tattttacaa
     7261 gcagaaaatc gattttatca tgaatatgat caaaggcatt tatccagtca ccgacgacaa
     7321 ttattgttaa gaacagctaa acagttcttg accgtcaaaa accggcatat ctggcggatg
     7381 actttgcgct catatgttaa gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc
     7441 tacctttaat ggaggcagat atttaagaga gcagttagat agtattttat ctcagctttc
     7501 agcagaagat gaagtagtca tttctgatga tgggtcagta gatgatacat gggatatatt
     7561 aactgaatac gcagaaaaag ataagcgaat ccagcttttt tcggggcctc gtcaaggatt
     7621 gatcgctaat tttggttatg cgatccagca gacgaaagga gatctaatct ttttggcaga
     7681 ccaagatgat gtttggttgg agacaaaagt gaaaaagatc actgactatt ttgagcagaa
     7741 tccggatcag ctagtagtag tttctgattt aatcattgtg gacagcgagc tgcaagagat
     7801 ccatgattcg tatttttcgt ttcgaaattc caaagaagga tggcttaata atgtgataag
     7861 aagtcactat attggtgctg gtatggcttt ccggtcttct ttaaaacccg atattttgcc
     7921 gatcccgtca gaagtgccga tgcatgatat gtggattggt ttgctgggag gaaagaagac
     7981 tgggttccta agagaaccgt taactctgta ccgaagacat gatttaaacg cgagtgagat
     8041 tgaaacacat tctaaattac ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt
     8101 gatcaaacga aaatggttta aacgttgata aaagatgttt aaatccttaa tatttccatg
     8161 acaaatataa caattgatga gataataaca aagtgaaatt tgaaatcaaa aaggagagca
     8221 gatatccatg aagggaatca ttcttgctgg gggcagcgga acacgtctgt accctttaac
     8281 aaaggcaaca tcgaaacaat tgatgccgat ttatgacaaa ccaatgattt attatccaat
     8341 gtctactctg atgttggcgg gaatcaaaga aattctgatt atttcaacgc cacaagatac
     8401 accacgtttc aaggaattat ttggaaacgg aaatgattta ggactgcaga tcgaatatgc
     8461 agtacaagaa agtccagatg gattggcaca agcgtttatc attggagaag aatttattgg
     8521 agatgatagt gtctgcttgg tattagggga caatatttac tatggtggcg gtctgtctaa
     8581 aatgttgcaa cgagcagctt caaaagatac aggcgcgaca gtatttggct atcatgtcaa
     8641 tgatccagaa cgctttggtg tagtagaatt tgatgagaat atgcaagctt tgtcgattga
     8701 agaaaagcct gctcagccaa aatcaaatta cgctgtaact ggcctttatt tttatgacaa
     8761 tgaggtaatt tctatcgcta aaggtatcaa accttcagaa cgtggagaac tagagattac
     8821 ggatgtcaat aaagcatatt tggataaagg aaaactatcc gtagaactta tgggacgcgg
     8881 atttgcttgg cttgatacag gaacacatga atcattgctt gaagcatcta cctttatcga
     8941 aacaattgaa aaacgtcaga acttaaaagt ggcttgtttg gaagaaattg cttaccgtat
     9001 gggctacatc gataaggaac aattgctggc ccttgcacag ccattgaaaa aaaatcaata
     9061 cggtcaatat ttattgaatt tggccgctga ataaggagtc cttacagatg aaagtaacag
     9121 atacaaaatt aacagatgtc aaaatcgtag aaatggacgt ctttggcgat catcgcggtt
     9181 tcttcacaga aagttattca aaagcaaaat ttgcagaaca tggattgaaa tatgactttg
     9241 ttcaagacaa ccattcacta tcagctgaag ctggtgtcct tcgggggctt catttccaaa
     9301 aaggagaagc tgcacagaca aaattgattc gggtagtcac tggagcagtt ttagatgtag
     9361 tagtggatat ccgcaaaggt agcccgactt atggtcaatg ggacggattt atcttatctg
     9421 aacataatca tcgacagttg cttgtaccta aaggatatgc ccatggattc gttactttga
     9481 caccaaatgt gaacttcctt tataagtgtg ataattacta taatgcagaa gcagatgggg
     9541 gcatcgcatt tgatgatcca gatttagcga ttgattggcc aattgatcca tcaagagcga
     9601 ttatgtcgga aaaagatcac cgtcatccga cattaaaaga atttgaagca gaaaatccat
     9661 ttgtttacgg agaaatctaa gaattaagga gaaattttct tatgaaaaac atcattgtta
     9721 ccggaggtgc cggattcatc ggctctaatt tcgttcatta cgtggtaaac aatcatccag
     9781 aagtgcatgt cactgttttg gataaactga cttatgcagg aaacaaagag aacttggctg
     9841 gacttcctag cgatcgcgta gaattggtcg taggagatgt tgcagatgca gaattagtgg
     9901 accgtctagt aaaagaagcg gacgcagttg ttcattacgc ggcagaatcc cataacgata
     9961 attcattaaa agatcctttc ccatttgtac aaacgaatat cattggtacg tatacacttt
    10021 tagaagcttg ccgcaagtat gatgtacgct atcatcacgt atctactgac gaggtttacg
    10081 gcgatcttcc attacgtgaa gacctgccag gacatgggga aggcgaagga gaaaaattca
    10141 ctgcggaaac accatataat ccttcaagtc catattcttc tacaaaagct ggttcagatc
    10201 tgttagtaaa agcatgggtg cgttcttttg gcttacgtgc aaccatttca aactgttcaa
    10261 acaactatgg tccttatcag catattgaaa aatttattcc tcgccaagta acgaatatct
    10321 taagcggtat tcgtccaaaa ttatatggcg aaggaaagaa tgttcgtgac tggatccata
    10381 cgaatgatca ttcttcagca gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct
    10441 atctgatcgg tgcagacggt gaagaagata ataagaccgt gatggaattg atcttagaaa
    10501 tgatgggtca accaaaagat gcgtacgacc atgtaaacga ccgtgcagga cacgacttgc
    10561 gttatgcaat cgattcgaca aaattacggg aagagttagg atggaagcct gagtttacga
    10621 acttccgtga tgggttggct gacacgatca aatggtacga agaacatgaa gactggtgga
    10681 aaaaagaaaa agaggccgtt gaagcagcat atgccaaaaa tggacaataa tacagagaaa
    10741 atgtcactgt attaatggaa aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa
    10801 aataaaaatc aaggagcgga taatcatatg tttttattaa ctggaggaaa tgggcaacta
    10861 ggtacagaat tgcgccattt attagatgaa aaaaatctgc aatatgtttc aacagatgca
    10921 aaagacttgg atatcacaga tgccgagaaa acaatggctt acatcacaga attaaaacct
    10981 gatgttattt tccattgtgc agcttacaca gcagtagata aagcagaaga agaagcaaaa
    11041 gaattagatg agaagatcaa tgttgatggt acaagaaatg tagcacttgc agcaaaagct
    11101 gtgaatgctc aattcgtcta tatcagtaca gactatgttt tcgatggaaa aaacaaaaat
    11161 gtagaatacg aagtagacga ccaaacgaat ccactaaatg aatatggtcg tacgaaatta
    11221 ttaggcgaaa aagcagtaga agagatttta gaagactatt atatcatacg aacttcttgg
    11281 gttttcggga tctatggaca caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc
    11341 gacaaactga ctgtagtaaa tgaccagttt gggcgaccaa cttggacacg tactttggca
    11401 gaatttatgg tatatgtcat cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac
    11461 gaaaatagct gttcttggta tgaattcgca aaagaaatat tgaaagatac agatgtagaa
    11521 gttgcacctg ttacgtcaga agaatttccg caaaaagcaa cacgtccgca gtattcagtc
    11581 atgagcctga aaaagacaga agagttagga tttgtcatac caacttggca agaagcactt
    11641 gcgcaaatgc tggaagctgc caaaaaaatg aataagtaat ttccttttat caaatagaat
    11701 ttggagcatg atatggaaaa acaaacaaga agtgcacgac gaaaagaaaa gccaaaagcg
    11761 aaccaggcag attcgaattt ggtgcttgac ctgtttctaa aactccgcta tcttattggc
    11821 gttttagtca tcgtggtcat cgttactttt aatttaaacg gaagttcact tggtgtttgg
    11881 gataaatatg tgtcgcaaag agacgatggg aaaaaaactg atgtgatttt tggacagaat
    11941 cgggaagtgc ggtcagacga atggctggtg cagacacctt tttacctctc acaagctgaa
    12001 gaggactttc cgttggtgaa cgaggattat tcattaaacg gacaaaacat gatcattgcg
    12061 tataattctc ctgtaaagga tattacggtt atcggaaaac cattcaactg gggattcttt
    12121 ttcctcggaa gagatcgagg actttccttt tattgggcga tgaaattagt cgtcatggtc
    12181 ttgcttggat tcgaagtact gatgatatta acgaagagaa aaaaagctct atcgctaatc
    12241 ggtgcatttg cgctgacatt ttctccagca gtccagtggt ggttcatgca acatgtgggc
    12301 gatttgattt tctttacttt gggactgatg actgcttttt atcattattt ctacaatcat
    12361 gataaaaaat ggctacgaac gttgatgatg cttttggtgg ttattttcgg tattgggttt
    12421 attttagtga tttacccagc ccatcaagtg atgctggcgt acttgctgat tttttatttt
    12481 gttggcctgc ttatttacta tagcaagaaa gtttcatggg atctgttgga tgtagtgttg
    12541 attggagggg cagttttatt tattggcgga gtcatggttc atttctggct aacgtcaaaa
    12601 gacgcattgt tagcttcttt gaatacctta tatccaggaa aacgggtaag tactggagga
    12661 aagtggacga ttggcgaatt tttttctttc cttacaaatt ggaaaattcc ttttgaagat
    12721 atcaactatt cgaataactc tgaagtggcc ctgttctatc acttttttcc aactgtattc
    12781 ctagcaagtc catttgtgtt atttggcaaa aaagatagcg aacaaaagtt actgggacgt
    12841 attttgatga ttttttgtct gttttctatc ttttggatta ccgttgggtt gccaaaatgg
    12901 ctagcagaaa tcactctgct gtcttatgtt cctccattaa gagccatcct tacttttagt
    12961 tttgcggctt gtttattatc gatctggttt atcgcttata tttggagaga agatgttatt
    13021 cctatttggt ataaattcat tttgttggca gtaaatgcat ggtcttattt ctatgcgatt
    13081 acgcattcga aaatggataa ttatttttcg gattttgaaa ccatcgcagt tgctgtgttg
    13141 ggaacagtga ttttagcttt tgcattattt ggctggagac gcttgtttta cctcgctttt
    13201 gccgccttag ttatcgtatc tggctttttc gtcaatccag tcgtagaagg tacaggagcg
    13261 atttttgaaa aaacactggc aaaagaaatc caaaaaatcg atcaacaaga tcctggtaaa
    13321 gtttggttgt ctgaagatga attatataac ttcaccccaa cattgggagt gaaatccttc
    13381 aatacagtac gcttttatcc agatatagct gcatggaaga aaatcgatcc gaaagaagaa
    13441 tacgagaagt attataatcg gtatgctcat acaagagctt ttatcgctaa ggaagataca
    13501 aagttcattt tagatcgtcc agatatgttt tcggtcacgt tgaattttga agacgcagaa
    13561 aaattaggga tcgactatgt gatcagtaaa cgaccattaa ctgattacaa ccaattatat
    13621 gaagcgcagt tcacgcagct atatggaccg gataaagacg gttatatgat ttttaaagta
    13681 gaatatccgg attatccaga tgtaccacaa acacaaactc aggaaaatca gctagtcgta
    13741 cagtaatagg ctagctgaga ataatcctga aatgttagta gaaaggagag tggagataat
    13801 tcaagcaatc gtacaaaaat ttgtcaacaa aatgagtgtc ggtttcttgc ttttgttact
    13861 gacgctagtc ttttttgttt ggacagcagg gttaggtttt ccagcaactt tgtttatata
    13921 taacaatagt tcattactta tcgcttttag tgtggctatt ttgctcattt taaatgttta
    13981 taaagcaaaa ggcgtcgatt ttctttatct aggtgttgcg cttgcccttt tcattttctt
    14041 ttcttacttt cgggaattaa gaaatgagtc tttatttgga gacttactgt ttcctttgat
    14101 tattttattt tgtttagtaa ttaaatggat cgagtggaca aaatgggatc gcggtattta
    14161 tctgattgtc ttttcggtct tcctcggaat cacgttatat cgtgtattta cagagatcca
    14221 agtaccagaa ggacaaagca tctgggctcc aggcaattcg ctttctgata tttggatcaa
    14281 tacgaataca atcggaagtt ctttgatgac tcttgcttta ttgatcagtg gatttgccag
    14341 ttcttttgaa cgatggtatg tacgaatcat cggtgcagta gcagttgttg cagcttttct
    14401 cgggatttgg gtctgccaat ctagaggtgc attagtcgcg ttgattgttt ttgcgatatt
    14461 ggatctttta cctaaatcgt tgatgcgtgt cattcgtgcg ccatttattg cttacacggt
    14521 tacgattctt ttagctttgc cgatttctta tctggcggct gtctctgaaa aagtcaattt
    14581 gtttaccggt cgagaagata tttggcataa attctatcag acgctaggag aaaaatctga
    14641 acaaatttta ctaggaatga aaacatttat cttccaacga ggaaaccaca atagttataa
    14701 ttctattttg aatatctatg gattgatagg atttgggatt gctgctttgc tgcttatcct
    14761 ttttattggt cgcttaacgt tgaaagcaga tttatccaac gggcagatga cttttatctg
    14821 ggcctttttt gcggtgatga tgcagtcatt tatggaggat acattgactt catacccatg
    14881 ggttccgatt atttatatcc tgttggctat ggccgttcat cgatatgatg aaccagaagt
    14941 tgcggaggaa ttctatgtta tggaagaggc atatgacgat atgacttatg atgatacatc
    15001 ttatgaggaa caaccagata gcgctcattc ttccaggatc caacgctatc attgataggc
    15061 acaaaatacg agagaggctg tgacaaccgt cgcagccttt tttggtttat tgcaattgtc
    15121 tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg tatcatagaa aattaagaaa
    15181 gaaataagaa cgcagctcgg taccaaaaat atggtaaaat ggcacttgac tgtaattcac
    15241 tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt atttatttat gaaggacatt
    15301 tatcagaaca gaaaattatt gctacagttt tcgatcaatg acttcaaatc aagatatgca
    15361 ggatcgtttt taggaatctt atgggcgttt atcaatccgc tatttactgt attgatttat
    15421 tggctggtat ttggtgttgg actgaaagca caaatgatcg atgcaaaata cccatttatc
    15481 gtatatttag ttaccgggat gattccctgg ttcttcttct cagatgcatt tgcatcaact
    15541 acggttgttt ttcgggaata tacttattta gtaaagaaag tcgtattcaa tattcgtatc
    15601 ttgcccacga cgaaaatcct atccaatctg tacacccatt tattcttcat tctgattggc
    15661 atagctgtcg cgcttataaa tggtatatat ccttcagcaa tgtctttaca gctgatttat
    15721 tatttatttt gcatgtgtgc tttcttgaca ggattgactt ggctgacggc atcgatccaa
    15781 ccatttttac ctgatatcat gcaattaatt acgatcgtgc tgcagttgat catgtggaca
    15841 ttaccgatat tatggagccc taatcagttt gattcaggtc ttgtaagcat attgaaatta
    15901 aatccgctgt actatgttgt tcaaggttat cgtgagtcat ttttaagtga agcttggttc
    15961 tgggagcatg gaacatatac tttgtatttc tgggttttca cgttagtaat gttcgtaatc
    16021 ggctctgcag tgttccgtag attgaaacca catttttcag atgtgctgta aggagaatag
    16081 ctatgacaga atatgcaatt agactaaaag atatcacaaa atcttataat atgtacgcaa
    16141 aaccttcgga tcgatttcgt gaagccctca atccttttaa gaaatcctat catgatgtat
    16201 tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg
    16261 gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat tacgggagta ctgacaccaa
    16321 catccggcac gatggaaatc gaaggaaaaa tctcagcttt gctagaacta ggatcaggat
    16381 tcaatcctga gtattctggc tatgaaaata tctatttaaa tggaatggtt ctaggatttt
    16441 caagagaaga agtcgatgaa atggtcgatg atatcattga atttgctgac atcggagacc
    16501 atatttatca acctgtaaaa acttattcaa gcgggatgtt cgttcgtcta gcttttgctg
    16561 tagcaatcaa tgtgaatccc gatattttga ttgtggatga agcattggca gtaggcgatc
    16621 ttgaatttca actaaaatgt atggaaaaat ttacagaaat caagaactcg ggaaagacga
    16681 ttttattcgt ttctcatgat atcagttcga ttcgtcgttt ttgtgatcgt tcctattggt
    16741 tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga agtaacgacg aattatgaaa
    16801 actttttaaa gaaaaaatca gttaaaacag tggatcggaa taaaaaccaa gtagaacggt
    16861 tcgccgctcc agatatcgtc gacgtgacga aagcggaatt gttggataaa gacaaaaatc
    16921 cggtggatat gctggaacaa ggagaaaaac tatacgttaa agtaacttat gaagtaaaag
    16981 atgatacaat caaaaatccg gttttaggaa ttgcgctacg aacagtcgac aatttttatg
    17041 tatgcggact taatactcta ctagatggta tcaagattcc ttggaaaaaa ggagagaata
    17101 tcttttatct ggagtatgaa aagttaggtt tgttagctgg tgaatattat tttgacgttg
    17161 cggtctttga agaaaatgca acggttcctt tggtatataa gacaaagtac atgaacttgt
    17221 ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt agaccataaa tgggaagaag
    17281 ggactcatag caatgaaata tgattttgaa atggacctag atgaacaaag cagtgtggga
    17341 aaaattgccg cacagatcaa accaggaagt aaagttttag agtttggtcc cggaaacggc
    17401 cgattaacga aacatttaat cggagcaaaa caatgtgaag tgagcattgt tgagttagat
    17461 aaggaattgt tcgattttgt cagcgaattt gctcaagatg gattttatgg tgatatcgaa
    17521 agttttgagt gggcaaacta ttatgctgga cagacatttg attacgtctt gtttgcggat
    17581 gttttagaac atttggtaga tcctggaaaa acattgaaga aagtcagaga atttttgaat
    17641 gaagaaggag agatcctgat tacattccct aacttggcgc ataattctgt catgatcgat
    17701 ttgttcaata accaattgcc gtgggcttct tatggtctcc tagatgaaac ccataattct
    17761 ttttatactc atgatggctt ccaaaaagtt tttgaaaaag cgggattatt cattaatatt
    17821 gaagattatt tgtacttagc tgtaggtgat acagaattaa aatcaactta tgaagaattg
    17881 ccggaagctg tacgctatga tttcaaaatg cgaccatttg gtgaagtcta ccaatacttt
    17941 ttctcattga tgaaacatcc agtagcacaa agttccattg cagagccaca aaattctaat
    18001 tatgtaaaag tgttagaagt aacccagcaa acgaaacaag atgaaacaat ccaacaaata
    18061 ccattcaata atttcacagg ggaaaatgag acactaagtt tccccgtatc agaaacaaca
    18121 gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta tcgaattcag cgcagaagct
    18181 gccggagaaa aactaacatt tatcgattcg aatgctgtag tcaaaacagt aaatgattgt
    18241 tatttgtttg atgggaaaga attaccagag ttcgttctaa cggatatttc tgggaaagaa
    18301 gtgactatcc attgtcacta tcgctttatc ggggaattga caccaacaat gaaagaattg
    18361 ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc tttctgagaa aaatgacgaa
    18421 ttagaaagag aaaatcatca tcttcttgag gaaaatacaa gactagacca tacgttaaaa
    18481 acaaccacaa atcgctattg cacattgttg gaaagtgatg aatggacaat aaaacataga
    18541 ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag aaaaagaact ttctatatgt
    18601 attgacgaga aaatctggga tgcagaaaca aaaatattaa aaatcatcgg atggggaatc
    18661 gctaattcgg atcgccagcc cttgtcctat aagttatcag ctgaccaatc gccatttttc
    18721 gaagcaattc ctgtttcacg agaagaagtc aatcaagcag aacagctcgc aaaaggaaca
    18781 gaagcgggat ttgagttacg aatcctttgc gagcaagaac gttcattctt agtggaagca
    18841 gtagctcaaa atggccaatc atggatcatt gaaatgtaaa taatgattaa cttataaaga
    18901 taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt atttatcgat caaatcactc
    18961 gtgaacgggc cactggcgat ttgcttattg tgggctgggc aattgatgaa gtgaccaaag
    19021 aaatccctac gataaaggta gaaaaagaaa atgtgattgc tgaagcaaca catgtcgtaa
    19081 gattggatat caatcattta tataatcttg atgttaagac ccaaagcggt tttaaggtac
    19141 gtttatcagg gaaaatgaga ggaaaagcca tcttagactt ccaaacagcg agacatcaaa
    19201 atggcatagc tgtgaaatta aacggtaaat acccttatga tgacggcatt gaatcttcat
    19261 gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta tgcaaggact catggcgtaa
    19321 aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg atcgatcgac tatgcagagt
    19381 ggatcagtcg tcatgagaaa ccagatttga aagagcaaag gaaagaagca caaggatttg
    19441 cgtaccgtcc attgatttcc atacttatgc ctgtctataa cgttgaaatc aaatggctcg
    19501 aaaaatgcat tgattcggta ctggatcaaa cgtatgatca ttgggaacta tgtatcagtg
    19561 atgatgcttc tactgatcca gctatccgaa agtgtctgga atcctatcaa gcaaaagatg
    19621 atagaatcaa agtcgttttc cgaaaagaaa atggacacat cagtctggca actaattctg
    19681 cattggaaat agcagaagga gaatttatag cactcttaga taatgatgat gaattaccgc
    19741 catttgcatt atatgaagtg gcaaaagtgc taaacgtaca tccagaactg gatgtgatct
    19801 acagtgacga agataaaatc gatgcagatg gcaatcgctt tgatccgcat ttcaaagcag
    19861 actggtcgcc tgatacattg atgggaaata attatatctc tcacttaggc gtatatcgaa
    19921 caagcattgt taaagaactt ggcggtttcc gtaaaggcta tgaaggttct caagattatg
    19981 atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca tatttaccac attgaccgag
    20041 tcttgtatca ttggcggacg atcccaggat caactgcaag taatggcgaa gccaaaagtt
    20101 atatctatga ttccggagtg aaagcattga cagatgccct gtctagaaga aacatcaaag
    20161 gaagcgtacg tcctggcaga atttctggat tttatgagat tgcttatgac gtcttgcaag
    20221 aggatttggt cagtgtgatc attccaacga agaatggcta tgaagatcta aaaacttgtg
    20281 tggattcgat catcgaaaaa acgtcttatt caaattatga aatcattatt gcggataacg
    20341 gaagtacaga tccaaaaatg caagaattat ttgcagaata caagcatcag ctgaaagacc
    20401 gctttatcgt agaattgatt gacattccat tcaattattc tcgaattaac aatttagcag
    20461 cagaaaaagc aaacggcaaa tactttttat tcttgaataa cgatacagaa gtgattgagc
    20521 cagattggat gacggtgatg gtttcttatg cacagtttga tcgaatcggc tgtgtaggag
    20581 caaaactgtt ttatccagat gatacaacgc agcatgcagg agtattatta ggaatcggtg
    20641 gtgttgcagg gcatgcattg aataactatg atcgaacaca ttgtggttat ttcggccgat
    20701 tggtcattga cgtcaattac ttagcagtca ctgcagcttg tatgatggtc aaggcagctg
    20761 acttcaatgc tgttaacggg tttgatgaaa cattagaagt agcattcaac gatgttgact
    20821 tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt
    20881 accatttcga atctaaatca agaggttatg aagatactcc agaaaaacaa agacgttttg
    20941 ctggtgaaat caaaaaaatg caagataaat ggccagcata tattgcgcat gatccatttt
    21001 acaatgataa tttaacaaaa gaaggaatcg gcgacttttc gttgagacca gattaaacta
    21061 taataaaaag ctgttatgtt gtgattcgct cgcacataac ggctttttat agttttttgt
    21121 caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg aggttgggac agaagtgttt
    21181 aactccgaga aataagaaga aattcacgaa aattgctttt caaatttttg tgaatttcgg
    21241 cttatttaag aagaagttgc ttctgctccc accgtttatt cgtttttaaa gaatgagaca
    21301 aaagtgtttc ttacttttgt cccacgctct tattcatgct cagagcgcca atagatacaa
    21361 aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa tcgatatttt attttaatat
    21421 aggctatttt aaaataaatg gtttaaggta ttcctctaca aaagccactc tatcaattat
    21481 catgggttta aaataagaag tgatggagat aaccagattg ttcttagcgt caatataaag
    21541 aacatttcca ctattaccaa ttgctgaata tatttcgttc tcagaatcta atacccacca
    21601 taaatagcca tatctcatat tttgtaagtc ttctaaagca ctggttctag gtttgaacat
    21661 ctcgtttatc cacatgggtg gtacgatttg ttttccttta tacatccctt tatttaggca
    21721 taatacacca atttttgcca gatcacttgc cgataaacac aaaccatatc ctgccgtccc
    21781 gatatttagt ggatcacaaa accagacatt ggttttagga gacttatcca ttgtaaactg
    21841 cttatgttct tcagctgttt cagcataata attgacatgg tttggtatcc ctataggatc
    21901 aaatagatac ttattggcaa aatcaacagt tgttagctta cttgtctgat acaacaagcc
    21961 agttaatatg tgtaaacaga cagtttgata gttgaattcg ccggttatac cttttcttcc
    22021 tcccaataaa tctagtgatg tttttgtcca atcttcactt gaacagactt ttgtccatgg
    22081 atcgccttta catttgtagg gtgctttcat tgttagcaag tgcttgattg taatagcgta
    22141 aatagttttc tccccgcgtt tcactttata ctctggaaaa taatctaata ctttatcatc
    22201 gatacttttg attaatcctt gctctaaagc aatgccaatc agcaaagata caatactttt
    22261 cgttgctgac atgatatgac aagtatcgtc ttttttataa ccattccatt catctgtata
    22321 aacttctacc ccatccttat atgcgacaat ttgacatata tttggttgtc gaaaaccaat
    22381 aaaatcatgc aactcttgtt tattcataag atattacctc actttcttct ataagcataa
    22441 tgtcattaaa aatcgatttc aattttttcg attcttcacg taatacaggt attcttatgg
    22501 actagacagc gctttgggaa gatggattct ctgaaaaata taaaggttca ctttatgagt
    22561 ggataaaaaa agcagcaaat acttcgactt ctttaattta ccaacattag tgaaaatcga
    22621 ttcatctgga gatttgtaat ttagagtcct tgtcttgatt agaggtgtat aaagcttttt
    22681 attagacatt tttaaattcc attacaattt gcttataaac taggttttta aaggtgacta
    22741 tggtaagata atagagatat tattttgtga ttaggagtgt atatatgaat aaaaatggag
    22801 aatggagtgc tgcacgacgg atattcatta tattaatgga tgttattgta tacaatgcag
    22861 caatatatag tagtttctta ctgaagtttc gtggagagat cccattacga aatttagaaa
    22921 ctttccaaca ctcagcaatt attatttcag tgatttttat tgcgttgaac attttgttgg
    22981 gtgcatatgt tttttataat cgaatgatca gtgacatcgt gtttgtcaca gtcatcggtc
    23041 aggtattgat gacactaggg atcatggtag tgacttttgc gggccgctgg tttgcttttc
    23101 cacgtcttgt tatcttactt tcatttgttc tcggtacgct tttcttaagt atctatcgta
    23161 ttctcgtata taaactttat ttacgcgtaa gtcgagataa aaaggtcgtc attattgggt
    23221 tagaaaagga tgtcgcacca gcaatccaaa attttggttc aaagaaaaac aacaaacata
    23281 aagtcgaagc agtcattctt gataactatt atgcaaacat caaaaatatg attaaagaca
    23341 tcgatattgt ctatcttgct tctcatatcg aagaaactga aaaaatcaaa atctatcaat
    23401 tagttactaa aaatgaaaaa aaattgtttt tgaatacaac atttgaaaac ttgacattga
    23461 tcaatccaaa tattatgaat ttcgaagatg aaagtattat tgaagcatct ggatttagaa
    23521 tccaacctga atatgaacta ttcaaacgtt tgtttgatat catcgtttca cttgttttat
    23581 tagttattgc ttcaccattt atgttattca cagcaatttt agtcaaagtg acatcaccgg
    23641 gaccaatcat ctataaacaa gtgcggatca caaaaaatca aaaggaattt tcaatctata
    23701 aattccgctc gatgaccgcc acagccgaag caaaatctgg accagtttta gctaagagca
    23761 atgatgcacg agtaacgcct gtaggtaaat ttattcgagc tgtccgcttt gatgaattac
    23821 cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt aggaccacgt cctgaacgtc
    23881 ctttcttcgt cgatcagttc aatgcagaaa acccttatta ctacttacgg cataatgttc
    23941 gtgctggtat cactgggtat gcacaagtct atggaaaata tgtctctgat tacaacagta
    24001 aattacgttt tgatttactt tatatcaaaa aatattcatt gatgcttgat ttaaagatct
    24061 tgctgcaaac catcaagatc ctatttgata aaatgtcctc tcaaggactt gaagaagacg
    24121 aaaatgtgat ctcattggca gaaattgctt ttccgaatga aaaaatcttt cgctagttgg
    24181 gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt accagtatac aatgcagaag
    24241 caactattgg tcgaacgatt gatattctaa aagaattgga ttatgaagag tacgaagtcg
    24301 ttttagtaaa tgatggatca acagatcaaa cacaggaaat cattcaagaa ctgattgaaa
    24361 atgatgaacg ttttgaattg attactacga ccaatcaagg gcccggatta gcaagaaacg
    24421 aaggaataag acagtcaaaa ggagaatatc tcttattctt cgatgcagat gacacgccag
    24481 taaaaacgat cttgtcagat tatgatcatt tgctagaaaa gagtccaaaa gcggatcttg
    24541 ttgttagttc gtttgttttt cgtacaatgg acgagggaag aatcgtttcc gaaaaagaaa
    24601 acttagttac agaatgtcac tatctaactc acgaagagtt tataaaagac atgtatcagt
    24661 taatgaatga acagttgatg tatgttgtat ggaacaaatg ctatcgtagc gatattctga
    24721 aaaataacca aattctattt aaaacttata atagttgtga ggatcgaata tttaacttac
    24781 attattatga atactgtcat caagtgatca tgaacccaaa agtagaatat atttatgaat
    24841 ttgaaggtgg gaaggggatc actaatcaat accgacctaa caagttcgaa acatttaaag
    24901 aattctattc attagcaaat actgtaacag acgatattaa taaagacggt atggcggcgc
    24961 ttcttttaaa agggacaact tcagttattt tttctattta tagtacctct gcaagaactt
    25021 caaaagagaa gaaagacgaa gcaaagcaga tattgagtga tccagtgatc cgagaagcca
    25081 agcaaattgc taagacggat tcttcagcaa aaaaaataac aaagttgctt tacaatatgc
    25141 cagctccact atttttatct gctgtaaaag ttggaagctt cgttgaagtg aaaatgccag
    25201 ggttaatggc tgctttgaag cgagtatatt agttaatgga ggaaaatata atgaaaagaa
    25261 ttttgcattt tcagggacga atgggtttag gtggagcaga gtctttcatg atgaatttat
    25321 atcgtaaaat tgatcgtaca aactatcaat ttgatttttt gatttatgaa gattatgcag
    25381 atgttcaaga ctatcattca gagattgaaa gattaggagg ccgtattttt gttgtaccta
    25441 atcctaagaa aaatattttt aaatatttaa tagaagtgaa taagttacta aaaaaagaat
    25501 ctttttctat agttcacaat caggtttatt ttggcggggg aattaattta tggttagcca
    25561 aaaagaatgg aatcagacag agaattgctc atagccatgc aactgaagat ggaaaatctc
    25621 aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa attgttgtta caaaatgcaa
    25681 cagactacct tgccgttagt caagaagctg gtgaaagtct gtttcaaaac catccctttg
    25741 agatagttca taatggtatt gatttagagc tttatagtaa aaattcagaa gctaaggtta
    25801 ataagagaaa agaattggat atctcaatga gtacttttgt tgtgggaaac attggacgat
    25861 tagagaaaca aaaaaatcaa tcttatttgt tagagatttt tttagaaata ctaaaaatga
    25921 attctgattc ttacttgatt ttagtaggtg aagggtcact tagaaataaa ctgaaagcca
    25981 aagcagtaga attggatata gagaaaagag taaaattttt aggagaaaga aacgatattc
    26041 ccgagttatt atctgtgatg gatgtttttg taatgccttc tctttatgaa ggtctgccaa
    26101 tggttggatt agaagcacaa gcagcaaaat taaaattagt cttagcagat actatttcca
    26161 aagatacaaa gttaacaaat aatgtggatt ttgtatcatt agatgaaaca ccacaagaat
    26221 gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat aacacaagaa ctaaagagtt
    26281 atgatgtttc gtacactact gaacaaatgg agagtatata tagtagagat cattaaaaat
    26341 gttcaagata ttttaggagt gattattttt gaaaatatgt tatttgatat tggcatacaa
    26401 tgatattggc aacttaaaga gattggtcaa tcgattgaac gaaaatgcag tttttattat
    26461 tcatatagat aaaaaaactg atatagaacc atttattgaa gcatttaaat catatgataa
    26521 tgttgaattt ttatgtaata aaaaccgtgt taaaatttat tggggtggtt tttccattgt
    26581 acaagcagaa atcaatttag taaagagagc attacaaaat gagaaatatt taaaatatgt
    26641 attattatct ggtgctgatt atccaatcaa agataatgag tatatttata attattttaa
    26701 aaagaatagt tcagtggagt ttattcgtgg aatagatcta gatcaaatta aacataaaga
    26761 attttattat aaacatatag atgtatatca aaaacatgat tatcctagaa ttaataggaa
    26821 taacacaact gcatttaaaa tttttagagc tataattaat cgttgtctga ggatgattaa
    26881 acttccccca aaaattagac accataaatt tgatttgtat catggttctc aatggtgggc
    26941 tttgtcgaaa gaatgcctaa cagaattaat acaaatgtat gaacaaaatc aacaagacta
    27001 cctgaatttt aaaataggta tgtttgcccc agatgaaaaa ttttttcata cacttttttt
    27061 taattcctcg ttcaaaaata aaaatgttat tggaggacca gatttaccac ttgaattgaa
    27121 aaatattgaa gaaactactt tacaaacttc gaaattagca aatattcata ttattgatcc
    27181 ttccatgaac aaatggtttg atgaaaggga tatagatcaa ataaaagaaa gtgaaaaatt
    27241 atttgtaaga aaagttaaat cggattactc tgataaacta ttaaatagaa ttgataaaga
    27301 aattttgaaa gttggaaata tagatgaata gaatagtacc tgttttagag aagagtttat
    27361 taagtcgaga tgcgtttcga gtaataaatt taatcatttg tctgctttat tttatgccta
    27421 gtacttttaa tgtaactaat atcccgatga aaatagcttt tgcttggggt ggaattatag
    27481 ttttgtatga ggtttttata aagaaaaatt tttttaaaat gcgtttttct tggctactga
    27541 ttggagcaat gattagtttt atcttaacta taatcattaa cagagaaaat tttttaattc
    27601 catctattta taattttgga tatttgctga ttactttgat agtaattttt cctgcagact
    27661 tttcacaaag tgaagaggaa aaaaagaaaa aaatggtgta ttttaataat gttttcatta
    27721 tcataatttt tttagctgcg ctaatatcta tctatcaatt tatttttttg atttcttatc
    27781 atgtcccaac aggaacgcca ggattacttg caagacaagg ttttattgaa catcggttat
    27841 ttggtgtcta tacaagtcca aatgttggag ctatgtttgg atatacttct attattttaa
    27901 gtttaattag tgtattaatt tcaaaaataa caaaaacgat tcgtgttttt tatatgatga
    27961 attttatcgt acaaatattt tatattacac tttcttcgtc tagaggtgcc caggtagtaa
    28021 tttgttcttt tttagtatta tttcttttgt tgtttgccaa ttcaaaattt agaaagagca
    28081 ttagagaagt actacctgta aaaatcaaat ggatggtaag catattatta gttctattgg
    28141 tatatttttc ttttggtact aattatgcta aggatttttt agcaacaatt cctgtatcga
    28201 ttgatagaaa agtagaagtt atagagtcaa atgaatcaga taaaactcca cttccagaaa
    28261 aagaagtaat attacagcat tctagtgata actccgaaat ttcagctggt cgttttacga
    28321 tttggaaagc tgcgcttaaa gttataagac caactttgtt atttggttat ggagagaccg
    28381 atttctatgg gaatagcgat gcaaaatttt tgaaaaaagt agatcttgat actacagatg
    28441 tgaatgaact aaaaagagca catggaaaca tgcataatgg atttcttcaa gtacttgtat
    28501 caagtggtat aattgctttt atatgtatct atgctttcta tgtgttaaat atctttagtt
    28561 tactcaaaac attttttaga agtaaagtga attatgcgtt tcttggtatt gttttaattt
    28621 ttatactatc tatttttata gatgaaatgg tggaggctca tgtacttttc aataagcgcg
    28681 atgtcatttc aattgttttc tggtattatt tgggaagcat ttcttacttg tatttaaaaa
    28741 aggagaatta atatgaagac agctttcatt gcttggactc ctttacattt gattaatata
    28801 ttaaatgttt atcaaaattt ttaccgagaa tcaaaaggag atttatttat ttattctgaa
    28861 tttgaaaacg ctagaagata tagtgagcgt ataaaagaat tagggctatt taatgatgtt
    28921 gtttttgtga atcaccggaa aatcggttct tcctttaaac gtaaaattaa cttgttaact
    28981 aaccataatc aatttttctc ggatgattta tccgtttatg atgaaatttt tatacagggg
    29041 gaaaattatt tcacaaaaat acttttctcc aatgtttaca aaaaaaatcc agatgtaaaa
    29101 ctaaattata tagaagatgg tttgggtact tatttgaaga agagtatatt ttcaacaagt
    29161 aaaaacgttc aaagagtttt taaaatatta aataaaaata gcatcttcaa aaaaaaaatt
    29221 gacgattatt ttgtctattg tccggaattg ttactttatg atgtaaaaaa tataaaaaaa
    29281 attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca tacaatcaat ctttgggata
    29341 gaagaagttt ttgtcggaga taaatgtatc ttttttgatc aacctttatt atctgatggt
    29401 tatggcatag atgaaacaga tattttcgct aaagtaaata gtatattttc tggaaaaaaa
    29461 gaaactttaa ttaagcttca tcctagagca aggacaaatc gttatggtga aaaaaacata
    29521 ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa atgacttctc ttcaacactt
    29581 ttgattagtc ctatatctac aatttctttt tcaccatcta tgatgtttga aaagacgatc
    29641 aactctatta tgttggctaa attaatttta aatgaatata cagcattatc tgaagataaa
    29701 aaagaaacat tgacaaatat tgttcttttc tgtgaacatt ataaccgtct atcagaagaa
    29761 aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt tggtagagta tgggattatt
    29821 taacacactt tccaagtcaa aattagttaa gtccagtttc tggtatacta tagggaattt
    29881 ttttattaag ggaatttcat tcataactat tcctattttt gtaaatatta ctactcaagc
    29941 agaatatggt ttgattaata atttcacttc atttgtatca attttttcga ttcttgttgg
    30001 gctaagccta aattcggcaa ttaataatgc aagttttgac tataaagatg acataaatga
    30061 atttatgtca tcggtacttt ctctagctac actttcgtta attgtgttct tattaatagg
    30121 gaacatatat tttttgtttt caaatgcttt ttttgatatg aaccaacttg tatttaattt
    30181 gatgttatta caaagttttg ctagttttgt aattagttat atccagtctt attttacgat
    30241 taactttaac cattttaaat atttgatttt atcttttata tctacttttt tgaatattgc
    30301 aatctctgta gctttgatgc agacattata ttttgatgat aaatatgttg gcagagtagt
    30361 cggcggaaca ctgtcatttg tgttgatagg tttagtaatt tatttaagaa taatgataaa
    30421 gggtgctcat ttttttgata aaaaacagtg gagatacgct ttaaaaatct ctttgccgct
    30481 aatcccacat tccttagcaa atatagtttt agcacaattc gatagaatta tgattaattc
    30541 ttatttaggt cctgctgcgg ggggaatata ttcctacatt agtaacatag gtattattct
    30601 aagcgtaata tggatgtcta ctaataacgc ctgggttcca tggttctatg gtgagatgag
    30661 taaaaataat gaaaaaaaag tgaagaaagt tagtaactat tatttagtag tctttacttt
    30721 gattacgatg attgtaatgg tagtttcgat tgatttagca aaatttatgg ctccgaaaga
    30781 ctattattct gggttatctt tagtggttcc gataacctta ggctatttct ttcagttttt
    30841 atatagttta ccagttaatg ctgaatttta tgagaaaaaa acatcgttta ttgcgattgg
    30901 tacagtagca agtgctatag ttaatatact attaaacatg attttcatac ctaggttagg
    30961 aataattgct gctggataca caaccgtatt tacgtatttt ttacttttta tttttcatta
    31021 taatatcgct aaaaaaatta cgaataaaca attatttgac acaaaaaaaa tatgtatcat
    31081 aataattttt gtatcaattt tttctttgat acttcatatt ttaatcaatc aaatgttgat
    31141 tcgttacgtt ttgacatcat tattgataat tagtttagtt atatattttt taaagattaa
    31201 gaatcgaatt atagatgact ataattcata ggaggagata taagtgttta aaggaaaaac
    31261 aatattagta actggcggaa ctggttccat tggaagtgaa attgttcgtc agcttttaaa
    31321 atatgaacca aaagctatca gaatttatag tagaggtgaa gaaaaacaat tttatatgca
    31381 acaagagctc tcaaatcaca ctaatttgag atttttaatt ggtgatatca gagactctca
    31441 aaggattgat tatgcatgtc gtgatgtgga tattattttt catgctgccg caatgaaaca
    31501 cgttccagca agtgaatata atcctatgga agctgtaaaa acaaatgtga tgggaactca
    31561 aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat tttgtaggta tcagtacaga
    31621 taaagtagtg agtcctacta acaccatggg tgcgacaaag ttattgagtg aaaaattaat
    31681 aatagctgcc aatcagtata aaggaaaaac agaaacaata ttttcttgtg taaggtttgg
    31741 aaatgtcatg gggtcaagtg gatctgttat tcctcttttt agtaaacaaa taaaaaacaa
    31801 taaagatatt actttgacag atgttgatat gacacgattt atgatgtcaa taccggatgc
    31861 tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt ggagaaacat atgtcttaaa
    31921 aatgccggca atcaatattt taactttagc tcaatcatta ataaaaatat ataatgatac
    31981 acatgatgaa aaatatagag gtgaaataaa agttgtaggt atcaggccag gagaaaaatt
    32041 atatgaagag ctaatgacta cagacgaaat tgaacgctca tatgaaaatg ataaaatgta
    32101 tatcattcca ccacactatc atgctactga cgaactgtac aaagattatg aaaaaattaa
    32161 aatcactgca tactcttcag atgcaacaga attaatgagt ggacaggaat ttattagaat
    32221 cgcaaaagag aataatgtcg tgacttggta aggagtttta tgatgaaatc agaagtatat
    32281 gtaattgcag aaattggtgt aaaccataat ggaagtctag atttagcttt agaaagtatt
    32341 gataaagcta aagaatgtgg agctaacgcg gttaaatttc aaacatttaa aacgaataag
    32401 ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg ataatcttaa agaggacagt
    32461 tctcaatttg aaatgctaaa aaaattagaa ttatctcaat ctgattttat tagaataaaa
    32521 gattattgta tacaaaataa aattgatttt ttgtctactc cttttgatga agaaagtgca
    32581 gactttctaa aacaaattgg tgtacaagga tttaaaattg gctcaggaga tttaacaaat
    32641 ttaccttttc ttagaaaaat agatatgtat gggttgccag ttttattatc tacaggtatg
    32701 gctgatattc atgaggtcag tgaggctgta gattgtttca tagatagtcc agttactatt
    32761 ttacattgta cttcaaatta tccggctagt tctgaagata ttaatttatt ggcgattaac
    32821 acattaaaag aaatgttcgg gatacccgtt gggtactccg atcattcatt gggatatgat
    32881 gttggtattt gtgctgttgc gatgggtgct aaggtaattg agaaacattt taccttggat
    32941 aaggaactac ctggtccaga tcataaagct tctttagatc ctcaggaatt tgcagatttt
    33001 gtaaagcata ttagaaacac tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct
    33061 tctgaactta gtacaaaaga agtatcaaga aaaagcctag tggtcactga gaatttaaat
    33121 gtgggagata ttcttacaga gaaatctctt acaataaaac gtcctggaaa tggaatagct
    33181 cctaagtttt tatatcaata tattggcaaa aaagttaaaa gaccaataag tgcagatagt
    33241 gttctgcttg aggaagatgt tgaatgaaaa aaatacttat agtaagtgga acacgagctg
    33301 attatggaat atataaacct gttgctgaga atttactaca agataattgg gatgtatcat
    33361 ttgttgtttc tggaatgcat ttatcccata aatatggatt tactaaagaa ttgatcttag
    33421 ctgacggatt tagaattagt ggagaagttc aaagtttgtt tttagagaac aataagggaa
    33481 acatggctcg gtctgtggca ttagagataa atggtttcac aaatatattt gaacaaacta
    33541 gccctgattt tgtattatta ctaggggata gaggagaaat gttggcagct gcgatagctt
    33601 gtttgtatct tggaatcaaa acagcacata ttcacggagg cgaagtgtct ggtactgtag
    33661 atgaatcaat aagacatgcc ataacgaaaa tagcatctgt tcattttgct gctacggaaa
    33721 aaagcaaaga gcgtttaata aaaatggggg aagattcttg gagagttttt cccgtcggag
    33781 ctcctagaat agatactatt ttaaataata gattacctag ttttagagat ataaaagaaa
    33841 aatatcatct aaaatttgaa aagaaaaaat acgttttgca agtatttcat ccagttgtaa
    33901 ccgaactaga aaatatagaa aaacaaattt tagctttgat caattcaatg gaacaacaat
    33961 ctcaaccaat tatttgcatt ttacctaata gtgatgctgg tagcgaaatt atcagaaaaa
    34021 tgtaccagaa atatttaaaa acggatatta tttatattac gaatttagag ccggaggaat
    34081 acctgactat attaaaagag tgcaaattta tgatcggaaa ttcttcttct gggataattg
    34141 aagctgcatc attcaaaata ccagtaataa atttaggaac cagacaaaat ggacgagaac
    34201 aatctaaaaa tattataaat gcaaatctaa atgaaaatga aattgaaact gctttgaata
    34261 aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt taagaacatc tacggtgacg
    34321 gcaacagttc tagaagaata acaaaaacat tgaatcaaat attgaatgat ggggaagata
    34381 ttaagtggat tcaaaaacaa atagcttatt aaaatatact agtgatccat acattattat
    34441 tggggctggc ggacacgcaa aagtcattat tgaaacctta agattagaaa ataagttaat
    34501 atatggattt tttgatgata agcaaaataa aatgcaagat ctgaaatatc ttgggaaaat
    34561 aaacgaattg aatagctggg cggatagcaa aaaaaaaatc tataaattta ttgtagctat
    34621 tggtgactgt gtagtaagaa aaaagattat aaaaaaattg aatatgccaa gtgagcaata
    34681 tggaatagct gttcatccaa gtgctatagt ggcaaaagaa atttcaattg gttatggaac
    34741 tgttattttt gcgaatgctg taattaaccc agatgctgta attggagagc atgctataat
    34801 aaacacaggt agcattgttg agcatgattg tagaattaat aactatgttc atttatctcc
    34861 aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga actcaaatag gaattggttc
    34921 tcaatgtatc cagatgaaga aaattggatc ttggtgtatg ataggcgcag ggagcactat
    34981 tgttaaggat attccctcac attccttagc ttatggcaat ccagctaaaa ttaagaaaga
    35041 aggaattaat tttgaattct agaattttat tatcaagtcc tcatatggga ggaacagaag
    35101 aaaaatatgt tgctaaggct tttaaagata attggatagc gccattaggt gaaaatgtgg
    35161 ataaatttga agaaagtatt acaaaatata ctggttctaa gggcggagct tctgcgttaa
    35221 gttcaggtac agctgccata catcttgcct taagactgct taatgttgga aaaaacgata
    35281 ttgtctttgc tccttcattt acttttatag ctacagtaaa tcctatttta tatcaagggg
    35341 ctgtaccagt gttcattgac tcagaaccta atacatggaa tatgagccct gttgcactag
    35401 agagcgcatt agaagaagct gaaagaaaca accaattacc taaggcaatc atcgtagtga
    35461 atttatatgg tcaaatggct gattatgaac gaattttagc tattgcaaac caatttaatg
    35521 ttccagtgat agaagatgca gcggaatcgt taggttctac ttataaggga atttcgtcag
    35581 gcttatttgg gacaattggg atctattcat ttaacggtaa taagatcatc acaacctcag
    35641 gtggaggagc tctagtgtct agtgataaaa aatatgttga gcattcaaga tttttagcaa
    35701 cacaagcaag agatcaagca ccgtattatc aacatagcga aataggttat aattaccgtc
    35761 taagcaatat ttctgccggt atcggacgtg gacaaatgga agtattgaat gatagagtag
    35821 ctaaacgaag agaaattttc aatcgatata aggaagcttt ttctggaatt gatagaattg
    35881 aaatgatgcc agaagtggat ggcatggtgc caaatcgttg gttgtctact ttaacgatca
    35941 atgattataa ttctattgat caagtagtta acgtaataaa taaactaaat gaaaaaaaca
    36001 ttgaaacaag agctttgtgg aaaccactac atttgcaacc agtatttcaa ggggtaaaaa
    36061 tgtattacca tgataataat cttagtgtgt cagaaaagtt atttaatagc ggcttatgct
    36121 taccttctgg aagtaatatg acagatagtg aacaagaatt tgttattgat gagtttaaaa
    36181 aaatattcaa gtaggtgtga tattttatga acataggaat tattggttta ggttccatgg
    36241 ggaaaagaag acttagactc attcaagaac actttaatga tattttacta tgtggtgttg
    36301 atactaatga ggcgagacga aaggaagttg aaaaggaatt tggaatacca acttataata
    36361 gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt ttttgtttgt acatccccta
    36421 taacccatga atttattata aaagaggctt taaatttaga ttgtaatatc tttacggaaa
    36481 tcaatttatt aaatgaatat tatgatgaaa taatcgaagt agctaaaaag aaagatagat
    36541 tactttatct atcttctaca tttttgaaga gaaaagaaat tcaatatatt aagtctaaag
    36601 tgacgcataa taaaaaaatt tcttatcaat accatgtagg acagtattta cccgactggc
    36661 atccttggga agattatcgc aatttttttg tttcaaacaa aaaaactaat gggtgtagag
    36721 aaatattcgc tattgaatta ccttggatta tagatacatt tgggaaagtt aaatcgttta
    36781 ctgtaatgac ccagaaatta tccaatcttg atattgatta ttcggacagt ttcactctta
    36841 ttattgagca tgaaactggt atagttggaa ctttaagtgt agatgttgtt tcaagagtag
    36901 ctaaaagaaa tttaactata attggtgaaa agattttaat agaatgggaa ggtaacccgg
    36961 attctctatt ttcatggaat gaagaaactt ctagtatgac agatattcaa ttgtacgatg
    37021 aggttatgca tgataataat tattcacata ctattattga agatgcatat ttagaggaga
    37081 ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc ccaatatact tttgaaaaag
    37141 ataagtatat tattaactta attaacgaca ttgaggtaaa ttaatgatga aagtaatttt
    37201 ttttgggtta gggtcaattg gaaagagaca tctaaaaaat ctaataagct atggaaaaaa
    37261 gaattctatt aattttgata taacagctta tagtagcagt ggaaaaaaag agtttgaaga
    37321 aataacgtat attcaaaata gagacgattt ggatacacat tatgatatag cttttattac
    37381 aaatccgact tttaagcatc tggaaacact aaatgtaatt gttgaaaaag ttgattacat
    37441 atttttagaa aaacctgctt tttctgagag tattgatctt actcaatata attatgatta
    37501 caaaaatatt tatgttgctg ctccactgag gtataaaaat atcatggaag ttgttcgtaa
    37561 tagtattcaa agtaaaaaag tatattctgt aagagctatt tgttctactt atcttcctga
    37621 ttggagaaaa ggtactgatt atagaaataa ttattctgcc tttaaatcta tggggggagg
    37681 agtagaatta gactgtatac atgaattgga ttatattata aacctatttg gtttccctaa
    37741 atcagtaaaa aaacagtttg gtaaatattc gaatcttgaa attaacagta atgatttagc
    37801 aacatatata ttgagatacg atgataaaat tatagaaatt catttagatt attttggaag
    37861 agttcctaca agaaaatttc aattgtttac tttagatgaa tgtatcaatg ttgacttatt
    37921 aaataataaa ataagttttg aagaaaacat tgttaaagaa atgcctgaat ctgctaatga
    37981 tatgtatgag aaggagttga aatatttctt tgaaaacgta gtaacaggca aagaaaactg
    38041 gaatgattta tatcatgcaa acgaagtatt gaaaatagct gaggagaaat aaatggaaat
    38101 tttatttact atttgtggta gagctgggtc taaggggttt aaaaataaaa atttgaaata
    38161 tttggcagga aagcccctag tccatttcgc tttagcaatg atagatatct atcaacaaaa
    38221 acatcctgaa cataattgca cgacttgttt gaatacggac agtaatgagt tagtagaatt
    38281 gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt aaacctgaat tagctacaga
    38341 tactgtaggg aaaattgaag tgatacgtga ttcattctta caactagaaa gagaagataa
    38401 tatatttgat ttaattatag atttagattt gacttcacct ttacgtgaag tttctaatct
    38461 tgaagaatta atagatgaac atctaaaaaa tttagataaa gacgtctact tttcagttgt
    38521 ttctgcaaga cgtaatccct attttaatat ggtaaaaaga attgaaaatg aagttactat
    38581 agttaataaa tcaagataca catctaggca acagacacct gaagtttttg aaatgaatgc
    38641 ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt aattatattt ttgatgggca
    38701 atgtgggata ataaaaatga gagattatct aattttagat attgatagtg aagaagattt
    38761 taaatggtta gaatttttgt ttcctaaatt tttagaagat tcttcggagc ttcaagatat
    38821 ctataattat ttatatcgga ggtaaaagga tgagctgtta tcttatcacc ggtggggctg
    38881 gcttcattgg ttctacctta gccaatcatt taggagaaga acatgtggta atcgtcgtcg
    38941 atgacttatc aatgggaaaa gtagaaaatc ttgatatgac gaaaaatatc acttttattg
    39001 agggagatgt cgctgagaaa tccttgatgg aagagatcat gaaagctaat aaatttgatt
    39061 atatctttca tttagcagca gttgctagtg tggcagattc agttgcccga ccagtagaaa
    39121 cacatcgtgt gaattttgaa agtgtattaa tgattttaga attgatccgc aagtatcaac
    39181 ctgatttaaa acggatcgtg tttagttctt cagcggctgt ttatggggat gaaccgactt
    39241 taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc ttatgcggtt gataaattcg
    39301 ctgcagaaca atatgtgcta gattattgtc atttatatga tgtacccggt agcgcagttc
    39361 gttttttcaa tgtctatggt ccaaatcaaa atccgaattc tccatacagt ggtgtgattt
    39421 ctattctcgt agatcgctat aagaaacaaa tcgtaggaga agcaacttca tttacattat
    39481 ttggtgatgg aagccagtct agagactttg tttatataga tgatgtaatc caagcattac
    39541 ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt caatgttgga acgggtaaag
    39601 caaccacact tttggaattg attcatgcaa tcaatgaaat tctcggtgta gaattgacac
    39661 ttgaatacaa agaagagcgt tcgggtgata tccatgactc tctagcagat attactaaga
    39721 tccagtcaat tggctatcaa ccgaaatatg gtattttaag tggaatgact aattatttga
    39781 aaactgaaat caattaaaac atactaagat aagattagag ggtaaattca ggctctttgt
    39841 caataaggac tgatgagttt tgtaaaatca aatctgggat agaa
//
LOCUS       DACCKU010000018        37707 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.018, whole genome shotgun sequence.
ACCESSION   DACCKU010000018 DACCKU010000000
VERSION     DACCKU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.018"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            212..2098
                     /locus_tag="IVN13_000853"
     CDS             212..2098
                     /locus_tag="IVN13_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7011362.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2110..3057
                     /locus_tag="IVN13_000854"
     CDS             2110..3057
                     /locus_tag="IVN13_000854"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7011363.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3077..3604
                     /locus_tag="IVN13_000855"
     CDS             3077..3604
                     /locus_tag="IVN13_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7011364.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3667..4320)
                     /locus_tag="IVN13_000856"
     CDS             complement(3667..4320)
                     /locus_tag="IVN13_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7011365.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            4452..5294
                     /locus_tag="IVN13_000857"
     CDS             4452..5294
                     /locus_tag="IVN13_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7011366.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            5452..6348
                     /locus_tag="IVN13_000858"
     CDS             5452..6348
                     /locus_tag="IVN13_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7011367.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            6351..7064
                     /locus_tag="IVN13_000859"
     CDS             6351..7064
                     /locus_tag="IVN13_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP7011368.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            7084..7602
                     /gene="msrA"
                     /locus_tag="IVN13_000860"
     CDS             7084..7602
                     /gene="msrA"
                     /locus_tag="IVN13_000860"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP7011369.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            7599..7826
                     /locus_tag="IVN13_000861"
     CDS             7599..7826
                     /locus_tag="IVN13_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP7011370.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            7978..8529
                     /gene="lepB"
                     /locus_tag="IVN13_000862"
     CDS             7978..8529
                     /gene="lepB"
                     /locus_tag="IVN13_000862"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7011371.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(8612..9154)
                     /locus_tag="IVN13_000863"
     CDS             complement(8612..9154)
                     /locus_tag="IVN13_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7011372.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(9391..9789)
                     /locus_tag="IVN13_000864"
     CDS             complement(9391..9789)
                     /locus_tag="IVN13_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7011373.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            10049..10909
                     /gene="ylqF"
                     /locus_tag="IVN13_000865"
     CDS             10049..10909
                     /gene="ylqF"
                     /locus_tag="IVN13_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP7011374.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            10902..11669
                     /locus_tag="IVN13_000866"
     CDS             10902..11669
                     /locus_tag="IVN13_000866"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP7011375.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            11725..12582
                     /gene="dprA"
                     /locus_tag="IVN13_000867"
     CDS             11725..12582
                     /gene="dprA"
                     /locus_tag="IVN13_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP7011376.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            12704..14782
                     /gene="topA"
                     /locus_tag="IVN13_000868"
     CDS             12704..14782
                     /gene="topA"
                     /locus_tag="IVN13_000868"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP7011377.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            14751..16127
                     /gene="trmFO"
                     /locus_tag="IVN13_000869"
     CDS             14751..16127
                     /gene="trmFO"
                     /locus_tag="IVN13_000869"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP7011378.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            16325..17230
                     /gene="xerC"
                     /locus_tag="IVN13_000870"
     CDS             16325..17230
                     /gene="xerC"
                     /locus_tag="IVN13_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP7011379.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            17267..17815
                     /gene="hslV"
                     /locus_tag="IVN13_000871"
     CDS             17267..17815
                     /gene="hslV"
                     /locus_tag="IVN13_000871"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP7011380.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            17829..19229
                     /gene="hslU"
                     /locus_tag="IVN13_000872"
     CDS             17829..19229
                     /gene="hslU"
                     /locus_tag="IVN13_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP7011381.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            19247..20038
                     /gene="codY"
                     /locus_tag="IVN13_000873"
     CDS             19247..20038
                     /gene="codY"
                     /locus_tag="IVN13_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP7011382.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            20120..20995
                     /locus_tag="IVN13_000874"
     CDS             20120..20995
                     /locus_tag="IVN13_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP7011383.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(21127..21765)
                     /gene="plsY"
                     /locus_tag="IVN13_000875"
     CDS             complement(21127..21765)
                     /gene="plsY"
                     /locus_tag="IVN13_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP7011384.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            21892..22326
                     /locus_tag="IVN13_000876"
     CDS             21892..22326
                     /locus_tag="IVN13_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP7011385.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            22492..24537
                     /gene="parE"
                     /locus_tag="IVN13_000877"
     CDS             22492..24537
                     /gene="parE"
                     /locus_tag="IVN13_000877"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP7011386.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            24549..26999
                     /gene="parC"
                     /locus_tag="IVN13_000878"
     CDS             24549..26999
                     /gene="parC"
                     /locus_tag="IVN13_000878"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP7011387.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKKYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            27276..29507
                     /gene="pflB"
                     /locus_tag="IVN13_000879"
     CDS             27276..29507
                     /gene="pflB"
                     /locus_tag="IVN13_000879"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP7011388.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            29751..30512
                     /gene="pflA"
                     /locus_tag="IVN13_000880"
     CDS             29751..30512
                     /gene="pflA"
                     /locus_tag="IVN13_000880"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP7011389.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            30597..31523
                     /locus_tag="IVN13_000881"
     CDS             30597..31523
                     /locus_tag="IVN13_000881"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP7011390.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            31705..32598
                     /locus_tag="IVN13_000882"
     CDS             31705..32598
                     /locus_tag="IVN13_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP7011391.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            32732..32857
                     /locus_tag="IVN13_000883"
     CDS             32732..32857
                     /locus_tag="IVN13_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7011392.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(32991..33623)
                     /locus_tag="IVN13_000884"
     CDS             complement(32991..33623)
                     /locus_tag="IVN13_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011393.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(33771..34313)
                     /locus_tag="IVN13_000885"
     CDS             complement(33771..34313)
                     /locus_tag="IVN13_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP7011394.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            34448..34837
                     /locus_tag="IVN13_000886"
     CDS             34448..34837
                     /locus_tag="IVN13_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011395.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            34841..35605
                     /locus_tag="IVN13_000887"
     CDS             34841..35605
                     /locus_tag="IVN13_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7011396.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            35734..36681
                     /locus_tag="IVN13_000888"
     CDS             35734..36681
                     /locus_tag="IVN13_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7011397.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            36811..37551
                     /locus_tag="IVN13_000889"
     CDS             36811..37551
                     /locus_tag="IVN13_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011398.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT        13283 a         6488 c         7876 g        10060 t
ORIGIN      
        1 tttccgattt gcgtttcgag caaagtcaaa aaaaacgagt ccaataaaat aaaaaggtaa
       61 aaattttcaa gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc
      121 tttttttgag aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt
      181 tatgatacaa tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct
      241 caaaaaaact tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa
      301 agaccgtatc gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat
      361 tgccggtatc gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg
      421 tattggttat ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt
      481 ctttcaagga aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga
      541 actaggggaa aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca
      601 aatgaataaa gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt
      661 aggaatcagt gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt
      721 tagtctggcg caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa
      781 ccatttagat tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc
      841 actattgatg gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga
      901 actttcattt ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa
      961 agcagaacgt gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca
     1021 ggaattagct tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat
     1081 caaccgtttc gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga
     1141 gctgaaccta gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta
     1201 ccgtataaat gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag
     1261 actaggtatt actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg
     1321 cagaattccc ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta
     1381 tacacaagaa aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc
     1441 tgctgaagaa gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg
     1501 atttttattt ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa
     1561 acgccggtta tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga
     1621 accgaccaat gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt
     1681 tccaggtgct gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa
     1741 gttattagta ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta
     1801 tttactgaag caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag
     1861 caaggaacct gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat
     1921 tgaagatgag attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca
     1981 tcaaggagat aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc
     2041 acaacttgaa gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga
     2101 ggaaaaagga tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat
     2161 cgtaaagaag acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt
     2221 gatctgcaaa aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa
     2281 agcgaattgc tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa
     2341 aaccatattt gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga
     2401 ccagatatga ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat
     2461 aaaaagcaga gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca
     2521 tacggcgatc ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt
     2581 ggctttatcg atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga
     2641 agattgatcg tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt
     2701 catacgatgt ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc
     2761 agtggggatg tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac
     2821 ctaattgcac atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct
     2881 catctttata tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca
     2941 tttccgactt tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat
     3001 atccaattag aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat
     3061 cagaaaggat gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg
     3121 aaaagaagac cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac
     3181 agaagcaaat acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt
     3241 gccgaatcgt cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca
     3301 tgtcatgcat agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt
     3361 catcgctgga gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg
     3421 tacagtcatc catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga
     3481 ttggtcactg atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca
     3541 atttgaaacg tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat
     3601 ataaaaaagc tatggataga ctagctgtta tttagagctg tcccatccat agctttattt
     3661 tttttattaa gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa
     3721 caaatgccaa acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat
     3781 cgtgtaagaa agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata
     3841 aagaggaaca gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac
     3901 ttttgatact ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc
     3961 aaacaaccag attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca
     4021 aaaaggagta taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa
     4081 cacctttttt gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag
     4141 aatcatcatc gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc
     4201 accttttact aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt
     4261 gactgcatta aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat
     4321 gaaaacggct cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa
     4381 aaatagaaat tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga
     4441 gagagaaaat aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa
     4501 atgtacgcga tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag
     4561 tatatcccga tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa
     4621 aaaatctgcc caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc
     4681 taggagaaga cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag
     4741 tagaagcagc aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg
     4801 ataccactga ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag
     4861 gtaaaacagt agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat
     4921 acatcggaat atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg
     4981 gtttgctgtc tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa
     5041 tccctgtaac taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa
     5101 atgaactgaa agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag
     5161 aattggcgca agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg
     5221 tactgcatac gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt
     5281 attatgtaga ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc
     5341 tctttttctt tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt
     5401 tgataaaatt tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta
     5461 acgaaacgac atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca
     5521 ggtttagctg cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga
     5581 gaagatccaa ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa
     5641 ggagtaggag atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa
     5701 gaccgttata atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt
     5761 gaccagatct taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct
     5821 gattttatca cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta
     5881 ttcgggttaa cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca
     5941 gaactattgg aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc
     6001 tacaatccat tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat
     6061 tggaacaaag gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc
     6121 aatgatttgc tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat
     6181 caaacaacta gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa
     6241 gatcatttcc atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag
     6301 ctgatcgaga cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa
     6361 gatctgacgt atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta
     6421 tccttcttgg ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc
     6481 gggaaccaga actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa
     6541 cgatcaattc aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc
     6601 aaaaagatag tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta
     6661 ctttcgaagt actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg
     6721 aaaacggaaa tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa
     6781 tcaaagatgt gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca
     6841 atacagatga tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt
     6901 atattaaagc agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc
     6961 caaaagatac ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta
     7021 ctgaaagcaa gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc
     7081 ataatggaac gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac
     7141 acgcttcctg gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg
     7201 acttatgaac aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat
     7261 gatccaacat tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca
     7321 actgacgctt tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat
     7381 ttgacagaag aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga
     7441 cgtttcgaac agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa
     7501 gaagaccatc aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc
     7561 cgggctgcct ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt
     7621 atttaatgac actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag
     7681 ccggtaaaga catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc
     7741 tggaactgaa tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat
     7801 atttagaaaa taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt
     7861 tttgtccaat agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt
     7921 tgatattttt cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg
     7981 acaaaaaaac aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct
     8041 gtattccttg cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat
     8101 tcaatggaaa acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc
     8161 agacgatttg atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta
     8221 atcggtcttc caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa
     8281 gtagttaagg aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat
     8341 acgacggact ttacactaca agagctaacg ggacaatcca agcttccgga agataattat
     8401 tttgtacttg gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat
     8461 aaaactgata ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg
     8521 atccagtgag aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc
     8581 caaaatcagt ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag
     8641 cctctaaagt tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat
     8701 tcctgatgaa tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca
     8761 gccaagaatt gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg
     8821 aaggcaggtt tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag
     8881 ccgtgataag aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt
     8941 tcgacaacat ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca
     9001 tacttaaaaa tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga
     9061 tgacatcaag ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca
     9121 atctaaaaac gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata
     9181 ataaaaggat accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg
     9241 gcatctaaca attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt
     9301 gtacgaataa aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt
     9361 gttataaaaa acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt
     9421 atttccgtct agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc
     9481 tacttcgtgg aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc
     9541 cgcattttca acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc
     9601 tttatctttt ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc
     9661 atcccatacc attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag
     9721 cttttcataa aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat
     9781 actattcatt tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa
     9841 cgctgtcaaa aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa
     9901 agtgaagatg tgatagaatg aggactggtt cactttcact taactaatag atggaatttc
     9961 tttataataa aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata
    10021 ttccggtatg aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc
    10081 aaaagcccga cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt
    10141 agacgcacga ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa
    10201 acctcgcctt gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg
    10261 gcaagaatat tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa
    10321 aggaattaaa ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga
    10381 tgctgctcgg gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt
    10441 tgggaaatct acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa
    10501 gcctggcgta acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga
    10561 cacaccaggg attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct
    10621 gacaggcgcg atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga
    10681 cttttttgcc cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca
    10741 agaagaaaaa ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga
    10801 agactatgat cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg
    10861 atacacactg gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa
    10921 gtatcaaaga acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa
    10981 gaaatgatcc acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa
    11041 aggaagcatt aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg
    11101 cacaaggtca ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac
    11161 ctgtcgtggc agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact
    11221 ccaaacaatt atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc
    11281 tggcaatcgg gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag
    11341 caagtaaatt agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt
    11401 tgatcgatgc gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg
    11461 cacgatctgt gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga
    11521 tgaaagaata tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa
    11581 caaaggaaca cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt
    11641 ttgcacctgt aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa
    11701 agagagacga aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct
    11761 tatacaaaag gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat
    11821 ccttataaag aaatcggatt tgaagacatc attaagatag gtaaaataac caaacaccgg
    11881 gagctgtttc gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa
    11941 cagtcgttta tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat
    12001 ccgccaattg tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc
    12061 gttgttggtg gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa
    12121 ccagtgatag aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac
    12181 ggtcaccaag cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata
    12241 gagcaagtct atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt
    12301 cttttgagtg aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat
    12361 cggattatcg cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc
    12421 tctctgataa cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga
    12481 aatattttaa cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc
    12541 acggtttcgg gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg
    12601 cttttcttga caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct
    12661 tttaatagta ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt
    12721 agtgattgta gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta
    12781 taaagtaatg gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga
    12841 ttttgaaaac aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa
    12901 aagcctgaaa gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag
    12961 agaaggagaa gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa
    13021 aaatcgggtg gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc
    13081 aagaacgatc gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat
    13141 cgttggttat tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg
    13201 acgtgtccaa tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt
    13261 tgttccagaa gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa
    13321 agcaaatttc tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa
    13381 agaagtcact gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga
    13441 gcgcaaaaga aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg
    13501 taagttaaac tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat
    13561 cactttgggt aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg
    13621 gattgccgat acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga
    13681 attttctaga catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga
    13741 agctgtgcgt ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa
    13801 agatcaattg aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc
    13861 agctatttta gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa
    13921 cggttcaaaa atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg
    13981 caaagaagag aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt
    14041 tgatattgaa ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt
    14101 gattcgtgca ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga
    14161 tacgatccaa agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact
    14221 aggagaaatt gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt
    14281 cacagcagaa atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa
    14341 agtggttgat cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat
    14401 cgaaaaaatt caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc
    14461 gatggtgata aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg
    14521 tcggaatacg aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg
    14581 acaagtcatt gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc
    14641 agaatgtgaa ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg
    14701 atatctggtc gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta
    14761 cgaagaagac atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg
    14821 ttggagcagg gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg
    14881 tccgtttata tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg
    14941 ctgaactagt atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat
    15001 tgaaagaaga aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag
    15061 tgccagctgg tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa
    15121 aagtcaaaga gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag
    15181 gaatcaccgt agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag
    15241 cattcaatgg ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag
    15301 ctacgatcga tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt
    15361 acttgaactg tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg
    15421 ccgaagtggc agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa
    15481 ttgaagtaat ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg
    15541 gattggaaga tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca
    15601 atgctgcagc ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc
    15661 aaaaacgtgt tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg
    15721 tgatgcaccg aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa
    15781 cgaaacgcga agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa
    15841 gtgcagcgag cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac
    15901 cagtcgagtt tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg
    15961 aaggaaaaca tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc
    16021 gcattcgaga caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg
    16081 aggcaataaa aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga
    16141 agagggtgag acggatagtc tcgctctttt tcttgcgttt ttcttaagaa tttctcaaac
    16201 aaaatgtgag gaattgcgcc aattgtgaac aaacttagaa atattggtta aattctgaca
    16261 atttatttgt ctttttataa acgctgtgct aaattattct ctgggacaaa aggaaggtga
    16321 gaaaatgaca gaagccacgt gggaacaaaa atttttgcgt tatttgattg tcgaaagagg
    16381 atattcagaa aaaacgaaag aagcatatga agaagacatt ctccatttca aacatttttt
    16441 ggaggaatct ggagacgctg atctgctgaa ggcggaatat tttacggttc gtgtctattt
    16501 gagtgcatta gctgatcatc gtcctagcta tagcagaaat tctatcagtc gcaagatagc
    16561 cagcctacgt tcgttttatc agtatcttct caaagaagaa gtgataaaag aaaatccatt
    16621 ttcttacgtt catctcaaaa agaaaaattt gagactgcca cgtttttttt atgaaaatga
    16681 aatgcaagca ctttttgaca gcgtagcagg agatagtcct ttggatttga ggaaccgcgc
    16741 tttactggaa gtcctttacg ggtcaggcat ccgtttaagt gagtgtagca gtttgtcggt
    16801 atcagacatt gattttgatt cagaggtcat gttgatccat ggtaaaggga acaaagagag
    16861 atatgctcct ctaggttctt ttgctcaaga tgctctgcaa gaatatttca gggaaggccg
    16921 ccaggtattg atgacaaaat atcacgaaga gcatgatatc gtttttgtga atcatcatgg
    16981 aaaaccaatc acgcctactg gaattgaata tgtcttgaat caaatgatca aaaaaagcag
    17041 tttggatagt gatatccatc ctcacatgct gcgccataca tttgctactc atttattaaa
    17101 taatggggca gatttgcgga cagtccaaga attgctagga catgccaatt tatcgactac
    17161 ccagatctat gcccatgtaa caaaagaaag tttacaaaaa aactatcgtt ctttccatcc
    17221 aagagcgtaa aaataaggaa aagaacaaat ttaggaggaa acaaacatgg tcgaatcacc
    17281 atttcattca accactatct gtgccgtcga aaaagacggg aaatttgcta tggctggaga
    17341 tggccaagta acaatgggtg aatctgttgt catgaaaggc actgcaaaga aagtacgacg
    17401 tatctacaat ggagaagttg ttgtaggttt cgccggaagt gttgcagatg cgtttacttt
    17461 agaagaaaaa tttgaaggca aactaaacga atataacggg aatctacaac gcgcagctgt
    17521 cgaattagct caagaatggc ggacacagca atctatgcaa aaacttgaag cgatgctgat
    17581 cgtaatgaat gataaagaaa tgcttcttgt ttcaggtaca ggagaagtta tcacaccaga
    17641 tgatggtatc ttagctatcg gatcaggagg gaattttgcg ttggctgcag cccgagcaat
    17701 gaaaaactat gctcaaaacg acatgagtgc gaaagaaatt gcagaaaatg cgttaaatat
    17761 tgccgcagat atctgtatct ttactaatca taatattatc gtagaagaac tgtaagaggt
    17821 gagtagatat gtatgaatta aaacaaacac caagacaaat cgtaagtgaa ttagatgaat
    17881 atatcgtagg acagcacgca gcaaaaaaat ctgttgccgt tgctttgcgt aaccgctatc
    17941 gccgtttgca gttagatgaa aaaatgcaac aagatattac accaaaaaat atgctgatga
    18001 tcggacctac aggggtcggt aaaacagaaa tcgcacgacg tttggcaaaa atcgtcaatg
    18061 caccatttgt caaagtcgaa gcaacaaaat ttactgaagt cggttatgtc ggaagagatg
    18121 tagaatcaat ggttcgggat ttagtagaaa atgcgatcca aatcgtagaa aaagagcagt
    18181 acagccgtgt atacacaagt gctaaaaaaa atgcagaaaa acgtttggta aaaatcttgg
    18241 tacctggtat caaaaaagaa cagaaacaat ccactaatca atttgaacaa atgatgaatc
    18301 tttttaatgc tagtcagcaa aataccgctg aaccgcaaga agaagtgaca gaagatattc
    18361 gaatcaatcg ccgtacagtc ttggaacaac tacaaaaggg tttactggat agtcgtgaag
    18421 taaccattga agttgaagac caaaagaaac ctgcaccaat gatgaacaat ggattggagc
    18481 aaatggggat cgacctaaat gaaactctag gtgctttgac accgaagaaa aaagtggaac
    18541 ggacggtaac agtcaaagaa gctttagagt tattgatcaa ggaagaatca gcaaaattag
    18601 tcaaggatgc tgatatccat agtgaggcaa tcaaattagc ggaagaaagt ggaattattt
    18661 ttatcgatga aatcgataag atcacttcta aatcacagca atctggagaa gtatccagag
    18721 aaggcgtcca aagagacata ttgccaattg tcgaaggttc acaagtaaac acaaaatacg
    18781 ggatcatcca aacagatcac gtcctattca ttgcttcagg agctttccat ttgtctaaac
    18841 caagtgatct aattccagaa cttcaaggac gcttcccgat acgggtagaa ttagatgatt
    18901 tgacagctga agattttgtg aagattctga ccgaaccaaa taatgcttta gtaaaacaat
    18961 acgtcgcgtt gatcggtaca gaaaatgtgt cagttacgtt tacaaaagaa gcaattgaac
    19021 ggatcgctca aatcgctttc gatgtcaacc gtgacacaga taatatcggc gcacgcagac
    19081 tgcataccat tttggaaaaa ctactagaag atctattatt tgaagcacca gatatgcaaa
    19141 tgggagaaat aacgatcaca gaagcatatg tggatgacaa acttggctca attgcggcaa
    19201 atgaagattt aagtcgctat attttataaa aaatcggagg acgacaatga cagacttgct
    19261 aacgaaaacc cgcaagataa acacactttt gcagcaaaag aatacttttg acatgcaagc
    19321 agatttgcct tacgataaaa tggctgtcac actaggtggt attcttgaca gcaacaccta
    19381 tatcatagga agtgacggag tattactagg ttataatgaa cgtcacgatg tgaacaatga
    19441 acgaatcaaa acaatgtttg ttgagaaaca atttcctgaa tcttatactg atacagtggc
    19501 acagttgatg aaaactgaag ctaatatccc aattactagt gatattactg catttccggt
    19561 agagtcaaga gagaaatatc catttggtct gacgactgtc gttccgatct ttggagctgg
    19621 cgaacgtttg ggtacgatta tcttatctcg tatggaacaa tcgtttgcag atgacgatct
    19681 agtcttagca gaatatggag ctacggttgt cggtatgcag atcctttatc agaagtctcg
    19741 aagcatcgaa gcagatgtac ggagtgcaac ggctgtccaa atggcaatca acacattatc
    19801 ttatagcgaa ttgaaggctg tacaagctat ctttgaagca ttggatggag atgaaggtcg
    19861 tctgactgct tctaatattg ccgatagtat cggcatcact cgttctgtga tcgtcaatgc
    19921 tttacgaaaa ttggaatcag ctggaatcat tgaatctcgt tcgttaggca tgaagggaac
    19981 ttatttaaaa gtattgaatt cccgttttaa agacgagtta gcaaaacaca gttattagaa
    20041 gtaaggggac tggggcagat agaaatgttt gttccagcct tcccatttta aaaagtaagt
    20101 cgaagttgga ggaatggcaa tgactgttat tattcaaaat gaccagctga tcgccgaaat
    20161 cgctgaacac ggtgcggaat tgatcagtct gaaatcaaaa gaacatggtt tagagtatat
    20221 ctggcaaggt gatccagcgt attggggcag acatgcaccg gttttattcc cttttgttgg
    20281 acgtttaaaa gatgatcagt atgtttatca agggaaaaca tatcaaatgg ggcaacacgg
    20341 atttgctcgg gatatggatt ttgaagtcat tgaacatgga caagaaatgg ctagtttcat
    20401 cttaaggagt acgccggaga cattagaaaa gtatccattt gattttgaat tagtgatcag
    20461 ctatgaatta ggaggagacg gcatcacggt tcattatcaa gtggcaaata ccggtgaaaa
    20521 agaaatgttc ttttctatcg gtggacatcc tgctttcaat gtacctcttg aagaaggatt
    20581 gtcatttgaa gatttctatc tggcattttc tccattgaaa tcaagattac gcctaccatt
    20641 gaaaggccca ttcatcgata tggatcaaaa gacgttaggt cagacaaata cgaatctggc
    20701 tttgaatcgt gaactttttg agcaagatgc cttggttttt gaaacaaggg gtctaaatgc
    20761 gtacgccatt cgaagtgaaa aatcttccca tagcgtaact ttaagctata aagatatgcc
    20821 ttatgtaggg atctggtctc cttatccaaa agaagcgcct ttcgtttgta tcgaaccgtg
    20881 gtgcggaatc gctgacacgg tgaacagtag tggaaacttg atggaaaaag tagggatcaa
    20941 tcaactaggt gcccatgaaa tcttcaagac aaaatattcc atcacagtga agtaagaggt
    21001 tgtgaccgag aagttagcag ggaacaccgt ttaccaaggt gtgtaaaaaa gctatcctgt
    21061 aagtaataag ggagtgtgac atcgatttgt cacactcccg ttttatctct ttggaatgaa
    21121 aaagtatcag gattttttct tgctgctatt caaaccaaac gggattcgac tttcagtatg
    21181 gtttctgata cgcttgatat tgtctttatg ccgataaaag ataaaggaag aaactaaaaa
    21241 ggcaatgatc gtcagtaacc aattgggctt agccaaaatc acaggagcta cataaggaag
    21301 gacgatcgta gataaagtga tcaatactgc actgatcata ctgctcaaac tcaccatact
    21361 tgtacaaaat aaactaacga taaagatgat acaagaataa acaaaaaata caggattaaa
    21421 tcctaaaagc attcctgcac ttgtcgcaac agcttttcct cctttgaacc cagcaaaaat
    21481 cggaaatgta tggccaagaa tggcgcatac cccaaaccaa agagggttaa tcccagaaag
    21541 gtggaagatc atcggcaaac aagtagccaa tgtacctttt aaaatatcca taagcaggac
    21601 aacaatcccg gctgttttgc ctaatacacg aaatgtattg gtcgttccag tattaccgct
    21661 cccgtgttgt cggatatctt ttttgaaaaa caatttgcca atccagacac cggaaggaat
    21721 agaacctaaa agatatgcta aaataagtaa taagatcaat ttcatttttt accctctttc
    21781 aagttgaaaa ctatccttta ttttagcatg aataggtaac aggaacaatt gaaaaaatga
    21841 aagatacaag tataatagat ggaataaaaa gaaaagcaag gggtggcaga aatgtctgta
    21901 aaaaatccaa cacaagaaac gattttcaac tatttggaag aagcaaaaaa tattgccgtc
    21961 gtaggcttga gcaataaacc agaacgaaca agttacatgg ttgcacaggt attgcaagac
    22021 aatggatata aaatcatacc agtaaatcca ttattggctg gtgaagaaat tctaggcgag
    22081 aaagtctatg cgaatttgac agaaatcgat gatccaatcg atatcgtaga tgtatttaga
    22141 cggagtgaat ttttacctga aatagcaggt gattttttga aaactgatgc taaagtgttc
    22201 tgggcacaat tagggattga aaacaaagaa gcagctgaga tgctaagaaa agcaggacgt
    22261 acagatatcg taatggatcg ttgtattaag attgaacttg gtaagttgaa agaaaaccaa
    22321 aaataaaaat gaccacaaag aagagatgat cgatagaggg gaatgtcgaa aaagcggtta
    22381 ggagataatt aaaattgatt tctcgtctac cctctttttt ttcacgacct tttctagtat
    22441 gataatcgtg gtgcggtttt gccgcaaagt attattgaag gagcgtttgc cttggcaaaa
    22501 aaagtaaaca acgaatacaa tgactcgtcc atccaagtat tggaaggatt agaggcagtt
    22561 agaaaaagac cagggatgta cattggatcc actgacagcc gaggcttgca tcatttagtc
    22621 tatgaaatcg tcgataatgc agttgacgaa gcattatcag gttatggcag tgagattgat
    22681 gtgaccatcc atgaagacaa tagtataact gttgcagata gcggacgtgg gatgcctgta
    22741 ggaatgcatg cttccggaat accaacagtt gaagtgattt ttactgtgct gcatgctggc
    22801 ggaaagtttg gtcaaggagg gtacaaaact tctggtggac tgcacggtgt aggagccagt
    22861 gtggtaaatg ccttatcgaa atggctaatt gtaacgatcg ttagagatgg cgtagagtat
    22921 cagcagaaat ttaaaaacgg cggtaaacct gacggtactt tgaaaaagat aggaaaaaca
    22981 aaaaaagcaa acgggacgac tgtccatttt ttaccagatg atacaatatt cagtacaacg
    23041 aaattttcat atgaaatttt agccgaacgg ttaagagaat ctgcctttct tctaaaaggc
    23101 gttaaaatct ctctttctga tcttcgtgga gaagaaccag tcaaggaaat ttttcattac
    23161 gaagaaggaa tcaaagaatt tgttgattat ctgaatgaag aaaaagatac attgacacca
    23221 gttgtttatt tttcaggaga aaaagaaggt atcgaagtag aggtcgctta tcaatacaat
    23281 gacggttatt cagaaaatgt tttatctttt gtcaataacg ttcggacaaa agatggggga
    23341 acccatgaag ctggtatgaa agcggcgatg acgaaatctt ataatgaata tgcgagaaaa
    23401 gttggtttgc tgaaagaacg ggacaagaat ctggaaggta gtgatttcag agaagggtta
    23461 gctgctgtac tttctatccg tgtacctgaa aatcttctgc aatttgaagg gcaaacaaaa
    23521 gaaaaacttg gtacgccagt tgctcgtacc gtagtagata acgtcatcag tgaacaaatg
    23581 ggcttttatt tgcaggaaaa tagcgaaatg agccagatgt tggtccgtaa agcaatcaaa
    23641 gccagagaag cgagagaagc agcacgtaaa gccagagaag aaagccgaaa tgggaagaag
    23701 cgtaaaaaag gcgaatctct tctttcagga aaattgacac ccgcacaatc ccgaaaccct
    23761 aagaaaaatg aattgtattt agtcgaaggg gattctgctg gcggatctgc taaacaagga
    23821 agagacagaa agttccaagc aattctacct ttacgaggaa aagtcatcaa taccgaaaaa
    23881 gctaaaatgc aagatatctt gaaaaatgaa gaaatcaata cgatgatcta tacgattggt
    23941 gcaggagtcg gtcctgagtt ttctatcgaa gactgtaatt acgacaaagt aatcatcatg
    24001 accgatgcgg atacagacgg cgcccatatt caagtattac tgcttacttt cttttatcgt
    24061 tatatgaaac cgctgattga agctgggaaa gtatatatcg cgttaccgcc tttatataaa
    24121 gtatccaaag gacaaggaaa gaagcaagtc attgaatatg cgtggacaga tgacgaattg
    24181 gcagcaatga ttaaaaaagt cggcaaagga tatatgctgc agcgatataa aggacttgga
    24241 gagatgaatg cggaacaatt gtgggaaaca acgatggatc cgacttcccg tacgttgatc
    24301 cgcgtccgta tcgatgatgc tgcgcaagct gaacgtcgag taaccacact gatgggtgac
    24361 aaagtagaac ctcgaagaaa atggatcgaa aatcacgtgc aatttacgct cgaagaagat
    24421 ggcagtatct tagataaaaa agaggatact gaaatctctc cttcagtttc taatgattta
    24481 ttagatgaag aacgagcaga taaaaatgaa aacaatcagt tatttgaagt agaataggag
    24541 gtcacctgat ggaaaatcga caagaagtac aagaattaac tttagaagaa gtgatgggtg
    24601 accgcttcgg acgttattcc aaatatatta tccaagaacg tgcactgcct gatatccgtg
    24661 atggattgaa gccagtgcaa cgccggatat tatttgccat gaacaaagat ggcaatacgt
    24721 atgacaaagg attccgtaaa tcagcaaaat ctgtcggaaa tatcatgggg aattatcatc
    24781 ctcatggtga catcagtatt tatgaagcga tggtccgcat gagccaagac tggaagttgc
    24841 gggaagtact gatcgaaatg cacgggaata acgggagtat ggacggtgat ccacctgctg
    24901 cgatgcgtta tactgaagcc agattatcca agttaagtgg agagatgcta gcagatatcg
    24961 agaaagaaac agtcgatctc gtgtggaatt ttgatgatac agaaaaagaa ccaacagtcc
    25021 ttccagctcg atacccaaac cttctcgtca atggatcgac aggtatatct gctggatatg
    25081 caacagaaat cccgactcat aacttagctg aagtgatcga tggcacaatc tatatgatcg
    25141 atcatccaca agcgagcctt gaaaaattga tggaatatat tcctggacct gactttccaa
    25201 caggagggat cttgcaagga aaagatgaaa tcaaaaaagc ttatgaaaca ggacgaggaa
    25261 aagttattct tcgttcgaaa acaaagatag aggcaatgaa gggtaataaa cagcaaatcg
    25321 tgatcacaga aatcccatat gaagtaaata aagcgacctt ggtgaaaaaa atggatgaga
    25381 tccgtttgaa taagaaaatc gatggaatag ctgaagtacg agatgaaagt gatagaacag
    25441 gtctgcaaat tgtcgtcgaa ttgaaaaaag acgccaatgc tcaaggaatt ttgaattatt
    25501 tgttcaaaaa cactgagcta cagatcaatt acaatttcaa tatggtcgct atcgaccata
    25561 tgactccgca tcaagttgga ctgaaagaca ttttaagaag ttatatcgag catcgaaagc
    25621 aagtcataac aaaacgtagt caattcgatt tagccaaagc tcaaaaaaga cagcatatcg
    25681 tagaaggatt gatgaaagct ttatcgattc tggatgaagt aatcgcaacg attcgtgaaa
    25741 gtaaagacaa aaaagacgcc aaaaagaatt tagtcgacgt ctttcaattt acagaagaac
    25801 aagcagaagc aattgtcaca ttgcaattat accgtttgac gaacacggat atcacagagc
    25861 ttcaaaagga atccgaaagc ttgattgcgc aaatcacaga gttgaataaa atcctttcaa
    25921 atgacaaaga gcttttctct gttatgaaaa aagaattgcg agaagtgaaa aaaaaatatt
    25981 cttccgctcg tctaacgaca atcgaagacg aaatcgaaga aatcaaaatt gatacgcaag
    26041 tcttagtggc acaagaagac gtaattgtca gtgtgacgcg agaaggctat gtgaagcgaa
    26101 caagccttcg ttcttatagt gcttccaaac cagaagaaat cgggatgcga gaaggcgact
    26161 acttgctata ctcaggtgaa ttgagtacat tggaccatgt tcttctgatc acaaataaag
    26221 caaatgtgat ctaccgccct gtccatgaac tacccgattt gaaatggaaa gatgctggtg
    26281 agcacatttc tcaaacaatc tccaatctgt ctgtagacga atcgatttta gctgttttcc
    26341 catatcaaaa aattcaagca gagaaaacat ttgtattcat cagcaagaat ggattgatca
    26401 agcaaacacg aatgacagag ttcgaaccat ggcgcaccta taagagccgt ccgctcagct
    26461 gtatgaagct aaaggcggaa gaagatgaac tgattgctgt gtatctgaaa gaagatcagc
    26521 caggcttaga tgttttctta gtaacgcatc aaggaatggg tttgcgctat cctttaacag
    26581 atgttcccgt tgttggagca aaggcagctg gagtgaagtc tatgaatttg aaagatggag
    26641 ataggattat caacgggctg cttgtgttag cagaaggtga tacgccgatc gtaatcgtta
    26701 ctcagcgagg agctgttaaa cgcatgcttg ctcaagaaat cagccaaact tcacgtgcga
    26761 agcggggagt gacggtcatg cgtgagctga aaaaaaatcc gcaccgtatt atctatatgt
    26821 cggaaggaag atcaaaagaa ataaccttga tgaatcaaaa agggcagcag ttaacgattg
    26881 acccaactga tttccctata ggtgatcgaa catcgaatgg atcatttgcg atggatgaga
    26941 aaaaaggtgg agaagtcatc gaagttatcg atgctcctga gattttcatc gagtcgtaac
    27001 tgttgtagta ttttaatgaa gagacgccat ctgtattaat acaggtggcg tctctttttc
    27061 attcattttt aataaatcct tatacgtttg caaaaaaaga tgaaagtgtt cacaatgctt
    27121 aaatgttggt atgtcaacgt tgatttaaat atatgtaaat aaaacataaa aattgttaaa
    27181 taaaaaacaa gaaaaccctt gcaaagatag ggtgctgtgt tatactaatc acgtaaaaac
    27241 tgttacataa aacagaaatt taggaggtcc atacaatgga gcaatggaat ggatttaaag
    27301 gtaccaaatg gaaagaaggc gtagacacta gagactttat tcaaaataac tacactgaat
    27361 atcgcggtga tgacagcttt ttggaaggcc caacagaagc aacagaaaaa ttatgggatg
    27421 aattgcaaaa gttaaatgat atccaattcg agcgtaacgg tgtctatgat atggacacag
    27481 aaatcgtatc aactattact tcacacgatg caggctattt ggataaagat ttggaaaaaa
    27541 tcgtcggtct acagacagac aaacctttga aacaagcatt catgccgttc ggcggaatca
    27601 atatggctaa caatgcgcta aaaagcaatg gctatacacc aaacgatgaa ttgacacata
    27661 ttttcactga ctggcgcaaa acacataatc aaggtgtatt tgacgcttat actgctgaaa
    27721 tgcgtgctgc tcgtaaaaac aaaatcatta cagggcttcc agacgcttac ggtcgcggac
    27781 gtatcatcgg tgactatcgt cgtgtggcat tgtatggaat cgactatttg atggaacaaa
    27841 agaaaaaaga tcatgatatg actggttatc atacaatgag tgaagaagtg attcgtttac
    27901 gtgaagaaat cactgaacaa tatcgtgcat tagcgcaaat gaaagaaatg gctgcgaaat
    27961 atggctatga tatttctaaa ccagctgcaa atgcaaaaga agctgtccaa tggctatact
    28021 tcggatattt agcagcaatc aaatcacaaa atggtgctgc gatgtcaatc ggacgtatct
    28081 cagcattcct tgatatctat atccaacgtg atttggataa tggagtaatc acagaaaaag
    28141 aagcacaaga attgatcgac cacttagtaa tgaaattacg tatggtcaaa ttcgcgcgta
    28201 cacctgaata taaccaatta ttctcaggat tccctatctg ggctacttta tccattgcag
    28261 gtatgggatt agatggtcgt tcattagtta caaagaatga tttccgtctg cttcatacat
    28321 taacaaatat gggaccttca ccagaaccaa acttgactgt tttgtattct gaacaccttc
    28381 cagaaggatt cagaacatat gctagcagaa tcgctatcga aagttcttcg attcaattcg
    28441 aaaatgataa tcagcttcgt gaacaatggg gttctgatga ttgtgcgatt gcttgctgtg
    28501 tatcaggaac agttgtcgga aaagacatgc aattctttgg cgctcgtgct aacttggcta
    28561 aaacactatt atatgccatc aatggcggag tcgatgaagt gactggcgcc caagttggac
    28621 cagaatatcg tccaatcaca tcagaaatac tagactttga agaagtgaaa cataacttca
    28681 tgaatatgat ggattggtta gcagaactat atgtcaatac gttgaacatc attcattata
    28741 tgcatgataa atattcttat gaagcagctc agctggcttt gatggattct gacttgcaac
    28801 gcacatttgc tacaggtatt gctgggatct ctcatgcggc agacagcttg tcagcgatca
    28861 aatatgcaaa agtaaaagca atccgtaatg aaaaaggaat agcagttgat tttgaagtgg
    28921 atggcgactt ccctaaatac ggaaatgatg atgaccgtgc agatgaatta gtttcatgga
    28981 tcttggaata cttcatggga caaatcaaac gtcataaaac ttaccgcaac tcaaaaccaa
    29041 caacttcatt attgacgatt acttcaaatg ttgtttacgg taaaaataca ggaaatactc
    29101 ctgatggccg tgaagctgga aaaccacttg cgccaggagc aaatccaagt tacaatgcgg
    29161 aacaaagtgg gttattagct agcctgaact caacagctaa aatgaattac ggctgtgcat
    29221 tggatggaat ttcaaatacc caaacaatca atccttcagg tcttggaaaa gaacaagata
    29281 caagaatcga aaacttgcgt aatgtcttag atggatactt ctcaaaagga gcttatcatt
    29341 tgaatgtcaa tgtattcagt aatgatttat tgcgtgatgc aatggaaaat ccaatgaaat
    29401 atccgaactt gacgatccgg gtatctggtt atgcagttaa attccgtgac ttgacaagag
    29461 aacaacaatt agatgtcttg atgagaactt cacacgacag aatgtaaaaa gttcgtgaaa
    29521 aaagtttttc gacttagcgt cgaaaggacg ttgaatagcc atcgcatcaa aatcgaataa
    29581 tcagtatgct tatttgaaag aaacgattta caggggcagg acttgtatgt tccgtccctg
    29641 taaattttac acataacgaa gcggcaattg cgaaaaattt tgttcagaaa gcttcgcttc
    29701 gacagctaac ttgtacttaa aatgaaatca aagaaaagat gtgaacttaa atggaagaaa
    29761 aaacaatcgg ctatgttcat tctatcgaaa ctttcggttc agtagacgga cctggactgc
    29821 gctttgtcgt gtttatgcaa ggatgccgca tgcgttgcca attttgtcac aatccagata
    29881 cttggaatat tggcggaggg aaagaataca cagcagatga acttttagat aaagcagaac
    29941 gcttcagacc ttactgggga gataaaggtg gtattactgt tagtggtgga gaacctctat
    30001 tgcagatcga ttttttgatc gaactattta aaaaagcaaa agagcggaag atgcatacta
    30061 cattggatac atgcggaaaa ccttttacgt atgaagaacc tttcttttca cgttttcaag
    30121 aattgatgaa gtatacagat ttactgttat ttgacatcaa acacatcgat aatgaagagc
    30181 ataaaaaact aacgcattgg gacaatgaaa atattttaga aatggctcaa tatttgtcaa
    30241 aaatcaacaa accggtttgg attcgccatg tactagttcc tgagcgaagt gattatgatg
    30301 aatatttgat ccgactagac aactttattc aaaccttgtc aaatgtagac agagtagaga
    30361 ttcttcctta tcatacgatg ggaaaataca agtgggaaac acttggtttg aaatatccat
    30421 tagaagggat cgaaccacct acaaaagaac gtgtcgaaaa tgctaaacga ttgcttcata
    30481 cagaagagta tacaaaatat ttaacgcgat aggtcgcatc ggaaagaaga taagtattgc
    30541 ttctttccgt ttttttgcta ttattaaata ggagaaatca caaaggagcg aatgacatgt
    30601 caaaaatttt agtttttggt caccaaaatc cagatacaga tgcaatcggg gcagccattg
    30661 cgttttcaca cttgcaaaat gaattaggta aagaaacaga agcagttgct ctaggtacac
    30721 ctaatgaaga aacacaatac gcattagacc actttcagct atctgcacca cgagtagtag
    30781 agtcagctaa agcagaaaat gcagatgtca tgcttgtgga tcataatgaa ttccaacaaa
    30841 gtatttcaga tatcagcgaa gtaaatattt tagctgtagt agaccaccat cgtattgcaa
    30901 actttgaaac agctaatcca ttgtactatc gtgcagaacc agttggatgt acaagtacga
    30961 ttattttaaa actatataaa gaaaaccaag taacaattcc taaagaaatc gcaggaatca
    31021 tgctctcagc tatcatttca gatacattgt tgttcaaatc accaacatgt acgcctgaag
    31081 atgaagcagc tgcaaaagaa cttgcagcaa tcgctgaagt cgatctaaac acttatggac
    31141 ttgaaatgtt gaaagcagga acaaatctaa gtgataaatc agctgcggtt cttcttgatt
    31201 tggatgcaaa aagtttccca atgggcagtt ccaacgttcg tatcgctcaa gtcaatacag
    31261 tggaccttaa tgaagtgatg gatcgacaag cagagctaga agcagcaatg gaagatgaaa
    31321 tgaaagcaaa tggctacgat ctgttcgtat tagttgtcac aaacatctta gacagtgatt
    31381 cagaattact tgttgctggt aaaggaaaag aaaaagtaga agaagctttc caaacaactt
    31441 tgaccaacaa ccgtgctttg cttaaaggtg tcgtttcacg taaaaaacaa gtcgtacctc
    31501 aattgacggc tgtatttagc taatcaaatg gtagtatgaa gcattcaaaa tgaatggata
    31561 agcgattgat ataaaaagaa acatagaaca tactaaaggc cgaagaaatg gaggagaata
    31621 acagtcaatt atgactcttg ttcttcctcc tttttacgta aaagcctttt aagtaaaata
    31681 aaacggatag aaaagagaac atatatgaac tattattcaa aaaataagga aaacttttat
    31741 caaaaactga ccgatgatcc gcttttttct tcattgaccg attatctata tgaacatcga
    31801 gaacaagaaa cgattctaag agaattgaaa aaagaatttt cgcaaaataa attttcccat
    31861 tttcttgatt tattgatcga tgcaggatta atcaagaggg aagaacgaag atatcatttg
    31921 aactttccaa tatttgatcc taaagattac ttgcaacaag ccacttcggc tgctgaaacc
    31981 atagctgaac aattgaagag actctctgtt gatgaacaaa aattagcgat gggagaagtt
    32041 atttgggcgt attgtttcga agatgaacgg aaagaagcgt atttttatgg agtaagaaac
    32101 agccgagaga cagaattgct gaggacaact gcaggaaatg agaagtaccg ttttatcaca
    32161 ctttcctcta tagaacactt cccgcttact ttggctaatt acttttttgt acagaagaat
    32221 cagcttcctg taacgaaggc attcaaagaa ttagccgaac ttattggaga cgtaaatgaa
    32281 tcgtactttt ttgatcaaat agaagttatc gttgacagga tacgtaagaa taaatataaa
    32341 aatcgccggc ctagtatttt ccatcagtct ctacttgtga cgaatactat aaaggaagaa
    32401 gaatcattca cattagaatt acctattgtc gaaaaaaaca attttgagat tgaattacca
    32461 acattggatc cttcattgac aatggaggaa atggcctttc ttaaacgaca aatcttttct
    32521 gagctgagca agaaatttat accgcatgca ttttcttata tcaaagaata tggaacagtt
    32581 ttagtgagta aaacctaaga gagcagtaag caaaaattat actgactctt gcaggaggag
    32641 cctttctctt cctttatggt taaaagaatt atttgagtat tttgcctatc taccacctat
    32701 tagattcact acatggtctg gaaacaaagg aatgcaaaaa aatggtgaga aatgtggaat
    32761 gacaaaggaa gtagtgataa gaaaagttag atttttaaat aatcaatatt atgattcagt
    32821 caagtatggc attttatggg aagaactagc tgattagatt aggaagtcca tttgataatc
    32881 cagaatcact aagaaacgaa tggcgagtca ttaataattt atccaatctt gggttattct
    32941 tttagattga agtgtttaaa actaaaaaag catttatgaa gtcgtaatag tcaggcttca
    33001 aaatgctttt tattttgttt gttttttctt tgccacataa atacacctag actgacgatg
    33061 aagagcatat ccaacagatt tcctgcgact tggatcgtcg aagggttggt cagtggcatt
    33121 aaagcaagga caaaaaagga aaacaagctg taaataaaac aggtgacagg aactaatcga
    33181 aagaagagcg gacatttcaa ttggctaaaa atgagtgaaa aaataaaata agtaaaaatg
    33241 gataaaatca cgactaaacc attcatgata aagaagaaaa gcgtattgtg attaaacgaa
    33301 taaaaaagaa ttgctaaaaa agaaaacgtt ttaaatgcaa tcaatacgct tgaagcttcg
    33361 tttaacgtac caggatgtcg atgaaaaaaa taggcaccga aataaacaga aaacagtaag
    33421 ccgcaaagag aagacaaaat caaaaaaaac ggattttctt cggcttctgt gatatcagaa
    33481 tgatccaaaa gcaaccagcc taatgcacta gaaggcgcat gtgaggtctt aaggtatgaa
    33541 taacatagaa aaaataagat cgtcaaagtt gcaaaaaaag agcctaaatg taaaaaatta
    33601 gtggttctac gttgaatttc cataatatct ccaaattaaa ttgataagta tataaagtat
    33661 aataacaaat tgatgagtat atgtcgatgt tttttaatca aaataataaa taaaaactgt
    33721 ctaacattta aaatttaatt tagatattag acagttttta agtttgtgag ttattttcct
    33781 tgtcgttttc tcactttttt agtccagaaa ccacctgaaa tatattttgg ctcataagaa
    33841 atgataaaag cacgctcttc gatttctttg atcaggcgat acagttcacg ttcgcttttt
    33901 ctaggagaaa gtatttcgag aactagccgc tcaccttcca gccccattgc agaagtctgt
    33961 gtcacaccat aaccgtgttc tctcagtata cccggcaaac ttttatcttc tgcgctattt
    34021 gctggcaaaa tcgttgtgac catgatgtat cctaatgcca atttgtcttc aatcaaaatt
    34081 ccgacactga tacctactgc gtaacctaac gcatagacaa ataaattcag tggattatct
    34141 aaacgatcta aaacaaggga taagccgacg atataaatcg tgatctcgac catactgaca
    34201 agtggagcaa tcagtcgata gcctttcatt gtcaacatga aacgtaacgt gttcagagtg
    34261 atataagcaa aattaatgag gaagatcatc cccaacattt ttggatcaat catttgtaaa
    34321 actcctttca tttatataac aacaatatca aaaaaaatcg taagtgaaga aaaattgaaa
    34381 ctttatacat acaaaaaaat gataaaatga ttataataga attataaaga taggttaggt
    34441 gattaaaatg aaaacggtca atcgtgtgtt ttgtgccagc cacgtgctaa gtctccttct
    34501 cagtatatac ttaggtgtct attcgattca acagccaatt gaggtagaga acattgtttt
    34561 tcagatgtcg ctgacagatg gtttaatttt aagcgttttt ttctttttga tactgtttat
    34621 tggtaatatt ttcggtagcg ttgccagttt tttgaatttt tctatttatc cactcctttc
    34681 gctagcatta ggagtggtgg gactttgttc tctgtgctta tttcccgagc cattcagtcc
    34741 tgcatttata ttatttggta cactgaacct gttccagaca actgttggag catggttgct
    34801 atggcgatca ggaaatttaa tgaaaatagg tgagtaacaa ttgaatccaa ttatattgaa
    34861 acgttctgtc catcatattc cagtgttaga agttgttctg aaagaattaa gagaggaacg
    34921 tattcccgtt gtcatctatt atcacggatg gcagacaagt aaggagttag ttttgacgca
    34981 aggaagaaaa attgcgcagc agggcattcg tgtgatcctg ccagatgcga tgaatcatgg
    35041 ggagcgcaaa cagccggttt cttctattcc atcctataca ttttgggata gtatctacgg
    35101 aaatctgttt gaatttgaca cacttatcaa gcatttagaa aaaagaggtc ttctacatga
    35161 tcagcttgca gttggcgggg tttcgatggg gggaatgaca acatgcgcct tgttggctaa
    35221 acatcctcat atagtagcgg gcgtgtgttt gatgggttcc cctgcacctt tggaatatgg
    35281 aaaagaaatc gtaaggcgcg catcggaata taattatgtc gtaccaaaag actattttga
    35341 tttgatttca tgggttaaga gctatgattt gtcattgcag ccggaaaagt tagctggacg
    35401 tcctctgttc ttttggcatg gacaagctga cgaaaaaatc ccttatatac aaacggaacg
    35461 atttgtagaa aaaaatcggc ataagtctta cggaaaagaa attgtttttc gttcctcaaa
    35521 aacacagcga catatggtac aaataccact aatggaagaa gcttccactt ttttagcaaa
    35581 aaatttgata aaaaagtata aataattgaa acggaaaatc actcatgacg atagaggatt
    35641 ttccgttttt tattttgggc tataataaac tctgttagga aaatttggta taattgatag
    35701 taagaatgac aacatgttag aaaggaagag agcatgccca ctttagcaga tgttgcaaaa
    35761 cgtgccaatg tttcaaaaat gacggtctct cgagtcatca atcatccgga acaggtgact
    35821 gacgaattga aagagttagt tttctcagca atggctgaac tggattaccg tccaaatatt
    35881 gctgcaaaag cacttgtcac gaatcggtcg cagatcatca aattatttat actggaagaa
    35941 atcgacacaa ctgagcccta ttacatgaat ttgctgatgg gaatcgctaa acggatcggt
    36001 aaagcgcatt attctctcca attagttaca aatgatgcct tcgacgtcgg ttcttgtgat
    36061 ggatacatca tcacaggtgt cagaaaaaaa gacttcgatt ggatcgaccg tttggaaaaa
    36121 ccagttgtct tgtttgggga aaattctgaa ggatatgatt atgtagacag cgataatgaa
    36181 tatggtacag caaaagcaac gcaatatgcc ttagaaagag attatcaaac gattatttat
    36241 atcggtatcg aagtggacga gccttttgaa atttcaaggg aaactggtta tttatctgtg
    36301 atgaaggaag ttgatcttcc tagtaaaatc ttgcggtttc caaatcattc gacagaagta
    36361 gaaagatata tgacagaaaa ctgggcagat tttacagata aaacttgttt tgtatgtagt
    36421 tcagatcggc tagcactagg agcagtaaga gggattataa atgctggagg acacttgccc
    36481 gaagaatttg gtgtgattgg atttgatggg gtatttttag accaaatcag ttcacctaaa
    36541 ttgacaacga tcaagcaaga tattatacgt atgggtgaag tatgtggaga aatgttgtta
    36601 aaaaaaatag aagaaaaagg ggcgagtcaa ggttatcgtc gtttttttcc tgaactagtt
    36661 atcagagaga caacgaatta agtcttatta ctatatatat atttatgaaa ttcatcgcta
    36721 ttttcttaaa ataatcagcg cattgctact gaaaacaatg aatctgtgct attattgaga
    36781 aaagttccat ttttgagaag gatggtttga atgaaagaaa tcagacgaaa acatttccgt
    36841 tttcctgcgt acgatgatga gttaggcgta aaattaacca acgacaagaa gcgagtatta
    36901 tttgacggac aagaagatct tttgactgaa cagaatcagt ccccgtttga acagatgaaa
    36961 gaatttcatt tcacgaacac gaacaccgag aaaatccgtc gcaagcgtcg tgcaacaaat
    37021 ggattgaagt cagctccgcg taaggaaaat ttggaaaagc ataaaggaaa attaccggat
    37081 tataccggcc attaccaatc agaaatcaca tcgacaggac gaaaaaaact ttttggtgat
    37141 tcctctcgtt ctgtcagaca aagtgaaaca aggacagagt ccaagcatac tacacgaaac
    37201 gcatctgttg acacgaaggt gcgggagcgt tcttattttg ttcctaaata tgtacccgca
    37261 tcgattatcc cagaccctag agaaagcaaa atttctgagg atcatttaat cgaatcgatg
    37321 aaaaaaacgg atgactctta tctgttgttc gatacagaac cgacgccgta tcaagaaaaa
    37381 agaaatggtg agccgtctgt acgaacattc aatcgaacaa aaacgttaga ggaaacaaag
    37441 cagtcagaag aaaaaaaatc gaaaaaaagc catggtatct tagaacgctc attacaagga
    37501 ttgatcgatg atcaaggaaa ctctttggga gaaaatagct attttaaata agtgatatac
    37561 aaatttatat tcggctacag ttggaaagga aacgctgcgg cgtttcctta ttttatactc
    37621 tatccgaaag tttatatcgt atcctcaaat ttttttgagt ttaagcgaat tttttctccc
    37681 aataagtaag aaaataatgg caaaata
//
LOCUS       DACCKU010000019        37071 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.019, whole genome shotgun sequence.
ACCESSION   DACCKU010000019 DACCKU010000000
VERSION     DACCKU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 37071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.019"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(45..275)
                     /locus_tag="IVN13_000890"
     CDS             complement(45..275)
                     /locus_tag="IVN13_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP7011399.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(361..1248)
                     /locus_tag="IVN13_000891"
     CDS             complement(361..1248)
                     /locus_tag="IVN13_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP7011400.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(1337..2560)
                     /locus_tag="IVN13_000892"
     CDS             complement(1337..2560)
                     /locus_tag="IVN13_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7011401.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(2577..3656)
                     /locus_tag="IVN13_000893"
     CDS             complement(2577..3656)
                     /locus_tag="IVN13_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP7011402.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
     gene            complement(3787..5514)
                     /gene="ptsP"
                     /locus_tag="IVN13_000894"
     CDS             complement(3787..5514)
                     /gene="ptsP"
                     /locus_tag="IVN13_000894"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP7011403.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(5514..5780)
                     /locus_tag="IVN13_000895"
     CDS             complement(5514..5780)
                     /locus_tag="IVN13_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP7011404.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            6259..8493
                     /locus_tag="IVN13_000896"
     CDS             6259..8493
                     /locus_tag="IVN13_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP7011405.1"
                     /translation="MLCQNCGKNEATIHLYATINGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8641..8961
                     /locus_tag="IVN13_000897"
     CDS             8641..8961
                     /locus_tag="IVN13_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7011406.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(9166..9390)
                     /locus_tag="IVN13_000898"
     CDS             complement(9166..9390)
                     /locus_tag="IVN13_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAP7011407.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(9588..11168)
                     /locus_tag="IVN13_000899"
     CDS             complement(9588..11168)
                     /locus_tag="IVN13_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP7011408.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(11569..12945)
                     /locus_tag="IVN13_000900"
     CDS             complement(11569..12945)
                     /locus_tag="IVN13_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7011409.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(13073..14191)
                     /locus_tag="IVN13_000901"
     CDS             complement(13073..14191)
                     /locus_tag="IVN13_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7011410.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            14333..14767
                     /locus_tag="IVN13_000902"
     CDS             14333..14767
                     /locus_tag="IVN13_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7011411.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(14845..15378)
                     /locus_tag="IVN13_000903"
     CDS             complement(14845..15378)
                     /locus_tag="IVN13_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP7011412.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(15472..16650)
                     /gene="mutY"
                     /locus_tag="IVN13_000904"
     CDS             complement(15472..16650)
                     /gene="mutY"
                     /locus_tag="IVN13_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP7011413.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(16643..17440)
                     /gene="recX"
                     /locus_tag="IVN13_000905"
     CDS             complement(16643..17440)
                     /gene="recX"
                     /locus_tag="IVN13_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP7011414.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            17547..18929
                     /gene="rlmD"
                     /locus_tag="IVN13_000906"
     CDS             17547..18929
                     /gene="rlmD"
                     /locus_tag="IVN13_000906"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP7011415.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(19103..19681)
                     /locus_tag="IVN13_000907"
     CDS             complement(19103..19681)
                     /locus_tag="IVN13_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP7011416.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            20003..20473
                     /locus_tag="IVN13_000908"
     CDS             20003..20473
                     /locus_tag="IVN13_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP7011417.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(20581..21807)
                     /locus_tag="IVN13_000909"
     CDS             complement(20581..21807)
                     /locus_tag="IVN13_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP7011418.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(22561..22761)
                     /locus_tag="IVN13_000910"
     CDS             complement(22561..22761)
                     /locus_tag="IVN13_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7011419.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(22895..23503)
                     /locus_tag="IVN13_000911"
     CDS             complement(22895..23503)
                     /locus_tag="IVN13_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP7011420.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(23588..24058)
                     /locus_tag="IVN13_000912"
     CDS             complement(23588..24058)
                     /locus_tag="IVN13_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP7011421.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(24106..25020)
                     /locus_tag="IVN13_000913"
     CDS             complement(24106..25020)
                     /locus_tag="IVN13_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7011422.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            25166..25969
                     /locus_tag="IVN13_000914"
     CDS             25166..25969
                     /locus_tag="IVN13_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP7011423.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            26147..27094
                     /locus_tag="IVN13_000915"
     CDS             26147..27094
                     /locus_tag="IVN13_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7011424.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(27162..27455)
                     /locus_tag="IVN13_000916"
     CDS             complement(27162..27455)
                     /locus_tag="IVN13_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP7011425.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(27483..28148)
                     /locus_tag="IVN13_000917"
     CDS             complement(27483..28148)
                     /locus_tag="IVN13_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP7011426.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(28288..28920)
                     /locus_tag="IVN13_000918"
     CDS             complement(28288..28920)
                     /locus_tag="IVN13_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011427.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(28927..29994)
                     /locus_tag="IVN13_000919"
     CDS             complement(28927..29994)
                     /locus_tag="IVN13_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7011428.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(29991..30722)
                     /locus_tag="IVN13_000920"
     CDS             complement(29991..30722)
                     /locus_tag="IVN13_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP7011429.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(30891..31370)
                     /gene="greA"
                     /locus_tag="IVN13_000921"
     CDS             complement(30891..31370)
                     /gene="greA"
                     /locus_tag="IVN13_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP7011430.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(31506..32681)
                     /gene="mltG"
                     /locus_tag="IVN13_000922"
     CDS             complement(31506..32681)
                     /gene="mltG"
                     /locus_tag="IVN13_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP7011431.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            32885..34054
                     /locus_tag="IVN13_000923"
     CDS             32885..34054
                     /locus_tag="IVN13_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7011432.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(34321..35943)
                     /locus_tag="IVN13_000924"
     CDS             complement(34321..35943)
                     /locus_tag="IVN13_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011433.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     TFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            36440..37071
                     /locus_tag="IVN13_000925"
                     /pseudo
     CDS             36440..37071
                     /locus_tag="IVN13_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT        11301 a         7361 c         6076 g        12333 t
ORIGIN      
        1 ttcttttagg catcttcaac ttcaaatgtc aattatccta aaatttattg gattaagtca
       61 tagatttcca tcgctacgat atcgatatta tcaaattcgt attgctgatg agtatctgtt
      121 tcagttaatt caaatgtatc ggtcgcttta tcataagtta ctgtacactt ctctacgcct
      181 tctttttcaa aacggcgaac ttgcagttca ttatctgctg actcgaccat agcctccagt
      241 cgttttatga ttgcgactaa ttgtgaatgt gtcatcggag gtcctccttt tcttatttct
      301 gttatattgt atcaaaagca tagcctactt gcattgattt tctctaaaaa aactcgtttt
      361 ttatgatttt ttttctggta tttttccttc aggccaccaa agtttgatca aagcttgggt
      421 tccgtcattc tcaaaacctt tatctgccaa ctcttcatat agtttcaatg cttgggtagt
      481 tgctggcaat acaagatcca ttttttctgc ttcttctaat gcaatcttta aatctttgat
      541 aaaatgtttg acaaaaaaac caggtgtgta atcttctttt aaaatacgag gagaataatt
      601 gcttaaagac cagttagctg cacttccacc tgaaagcgtc tcaatcactt tctttaaatc
      661 aagtccgttt ttttgtgcat agacaagcat ttccgtcatt cctgtcatcg tacctgcaat
      721 catgatctga ttagccattt ttgtatgctg acctttccca gcagaaccat gcaatgtaaa
      781 tgtcgtacca aattctttga ataaaggcag tacttgatca taaacggctt cttctccacc
      841 gaccatgatc gtcagtgtac cattctttgc tcctaagtca ccaccggata ccggtgcatc
      901 taacgcatct gcgccttttt cttttgctgt ttttgcaatt ttttctgcca ggcttggtgt
      961 acttgtagtc aaatcaacta aaatatgtcc tgagatatct gcagaaaaaa tccccgactg
     1021 tccatagtag atttcttcta catctttggg gtaacctacc attgtaaaga gaatattact
     1081 ttgttctgct accgcttttg gtgtatctgc ccagatagct ccttctgtta ctaaaggatc
     1141 agcttttgct tttgtccgat tatagacagc tacttcgtgt cctgcttcta gtagatggcg
     1201 tgcgacagca cttcccatga cgcctgttcc gataaaacca agtttcattt tttcgacctc
     1261 ctaaaaatca aaaatccttg cttctatgct agtatacgct agaaacaagg attttttcat
     1321 aaaaaatgtt tgaattttat cttttaaatg aatttaattt tattttgatt cgttcaatcg
     1381 agtcttcttc ttcttttctt cgcatctctt cttcatgata aacaaccatg tcttgataaa
     1441 attcgatcat agcttcgccg aaatgtgtag atgaaatatc ataaagttta gcttctaaaa
     1501 cttttggatc tttaggaact tcactggaaa tataatcaat aagtgctggt gcaaagtctt
     1561 cgtcggtttc aaaagttttc cctaatgaag gatgatcgaa caatcggttc aaataagcat
     1621 ttccttccac gacacaaggt gttccggctg ccatcgcttc tgtataagta agcccttgtg
     1681 tttctgaagt agaagcactg acaaaataat ctgccgcttt ataataaagt gctacatcat
     1741 tgttgtccac ttctcctgtg aactgcacaa attcttctaa ctgcagttct gagatcaatt
     1801 cctctaactc ttctacataa ggtccttttc ctacgataac taatctggcg ttaggataag
     1861 cggagatgac ctgaggaaac ccattaatca aggcttggat atttttctca taagaaatcc
     1921 tgcttaaaga aagaatcatc aaatggtttt cctgaagacc tagtgactcc cttaatttgc
     1981 tgatatcctg ctgagtgata tcagaacgtt tgaatttttc aatgtcgata ccagtaggaa
     2041 tcacgcgaag cggtgctatt acaccatagc tgcgtaatgt atcaataaca cgttcacttg
     2101 gacaaactac acctgtcgta tgatttgcaa ataatctaga caagtatttt acatgggatt
     2161 gcctgatcac ttttccttta ccgatataat gaaggtagtc ttcatacatc gtatggtaag
     2221 tatggatcag aggtattttc aacttcttag ctaccatctt cccaagtatc cctgctccga
     2281 attctgtatg ggtatgaata agatctagtt ctagttcttt ggcaaccaaa taagcatacc
     2341 acatgcctct aacaacaact cttcgatcct tgaaagaaat aaatggtaca cttggcattc
     2401 gaatcacatc tttttccaac ttatccgcat taggatcggt cgttgtaaaa atatagactt
     2461 catgcccatg tctttctaat tcttgtttta atgttttgat cgatgtagca actccgctga
     2521 cttgcggaaa gtaggtatcg gtaaaaaagc cgattttcat gtttttcgcc tccaaactag
     2581 tccttgacct gatttacttt ttttaatttg ctagagactt tttcattcag tacactttcg
     2641 tatacatttt taagttcttc accgatttta ttgatacttc gctcttttgc tgtttgataa
     2701 ccggcttgtg tcgtactcgg tagttcttga ttggtaatct gttggatcag tcggataaat
     2761 tcttcgtttg tcttccccat atagcagttc ttgctatctt ctaaccagcc attatacact
     2821 ggaatatctc gaacaagtac gttttgtttg cttgctaaag cttctaaaac aacgatccct
     2881 tctgtctctt cataagaagg gaaaaagaat agatctgcac ctgaatacgc accttctatg
     2941 atctctccac gaatgtaacc aggaaacagg acattttcag gatgatcttc ttttacaatt
     3001 ttccggatgt tccgaggaat cgagtacatg ggaacatgac caaaccaaat aaatgtgtat
     3061 tcgggcatct tcttagcaat ttctacgaaa tcaatgattc ctttacgttc gaaaaacaaa
     3121 ccgacacaaa tgattacttt ttgatccgga gacagtttga aatattctct gaacttttgt
     3181 tctttcattg gatcagggtg atatttctct aagtcgatcc cattggaaat agcttgaatc
     3241 ggcacacgga taccatagct ttccaacaac tgttttgaat aaggtgttgg cgtgatcaga
     3301 tagtccgctt tttggtacaa tcctaccaga tattttttta cgataggaga aagctggttc
     3361 gatccgataa aggaatttcg gaaatcttcc tctgtagaat gcgcatgata gacaactttt
     3421 tttccttctc ttctggcttt attgaccata ttatgactat tgattccgta tgtattgatg
     3481 tgcaggatgt cataatcttc tttttcatct agtgtataag agataccgac ttccgtcaga
     3541 gcacgttttt gatgatcaag cgcacgcccg attccagatt ttgcaagcat cttttcactt
     3601 tcaaaatata ataatacctt caccttacgt cccccttcac ttatatagta taacatagcc
     3661 tacaaactgt cactcaaact taagccctat ttttgtctat tcttactgac aaaaacgttt
     3721 tcttaaaaaa gaacaaaaaa actggcgtag ctcttttaca agccacacca gcttttgata
     3781 cgttatttat tttacatatt catgaacaag ttcaacgact tcttccatca tatcgcattc
     3841 tttcaatgca cgatcagcaa gttcagccat tttcgctgtg tccaagcgtt tcattaaact
     3901 acgcgtttta agaatagatg tcgcactcat tgagaactca tctagaccca tacctactag
     3961 caatggaaca gctgtttgat ctccagccat ttctccacac atacctgccc attttccttc
     4021 agcgtgtgct gcatcaatta catttttgat caaacgtaaa attgatgggt tatatggttg
     4081 gtacaagtaa gaaacgcgtt cgttcatacg gtctgctgcc attgtgtatt gaatcaaatc
     4141 gttcgtacct acagagaaga aatctacttc ttttgcaaac ttgtctgcta agactgctgc
     4201 tgctggaatc tcgatcatga tgccgacttg gatcgtatca gatacttcct tgccttcact
     4261 tatcaatttt tgtttttctt cttcaaagat cgcttttgct gcgcggaatt ctttgagtgt
     4321 agctaccata gggaacatga tacgtaaatt accatgaaca gatgcacgaa gcaatgcacg
     4381 catttgtgta cggaacattt cgtcgccttg ttcagataaa ctgatacgca atgcacgata
     4441 tcctaagaat ggattcattt catgaggtaa ttgcagatat ggtagttctt tatctccacc
     4501 gatgtccatt gtacgaacaa ctacaggttt tccttccata ccttctaata cagctttgta
     4561 agcttcatat tggtcatctt ctgttgggaa gtctggagaa tccatataca ggaattcagt
     4621 acggtacaga ccaactgctt ctccaccatt attatggaca ccaactagat cttttggtgt
     4681 tccgatgttt gcagctaatt caaaatgctt gccgtcagct gtgacagttt ctgcattttt
     4741 taatttttcc cattctgctt tttgtgctgc gtaatctgca cctgcttttt caaattcagc
     4801 tttttgttca tctgtaggat cgatgattac atcgccttcg atcccgttga cagccaaaat
     4861 atcgccttct ttgactttag cagtaatttc ttttgtccca acgattgctg gaatttctaa
     4921 agaacgtgcc atgatagcag aatgtgaagt acgtccacca atgtctgtta caaacgcttt
     4981 tacaaaattg cgatctaatt gtgctgtatc acttggagtc aagtcatgtg caacaacgac
     5041 cacttcttcg ttgatcattg atggatttgg taaagtaacg cctaataagt gcgctaaaat
     5101 acgtttagca acgtcacgaa tatctgcagc acgctcttgc atataagcat tatcttccat
     5161 tgcttcgaac ataccgatat acatatccgt tacttctttt agcgctgatt cagcatttac
     5221 gctgttgtct ttaatatttt gcttgatttg acctaccatt tctggatctg aaagaaccat
     5281 caaatgggca tcaaatactt gcgcttctgc ttcacctaag ctttgcgctg ctttttcacg
     5341 aatttgctgc aattcttcag tagatttagc taatgcagca tccaaacgag cttcttcagc
     5401 cgcagtatct tctactgttg ccttactgaa tgttaaatcc ggttgaacta gcaggtaagc
     5461 ttttgcaacg gctacaccgt cactagcggc aatccctttt agcatttcaa ccattattca
     5521 gataagcctt ctttcttcat tgtttcaacg attgctgcca ttgcatcagc ttcgtcagca
     5581 ccttctgcag taatagtaac atcagaacct tgacctacac ctaaagacat aacgcccatg
     5641 attgatttca agtttactga tttaccttta tactctaagt ttacatcaga gttaaatttg
     5701 cttgctgttt gtactaatag tgtagctgga cgagcgtgga tcccagtttc tgctactacg
     5761 tgaaattctt tcttttccat attaatcggt ctcctttaag tgagtgttgt ttttatgtga
     5821 gggttttctt ttttagtaat tcagagaatc ctaacaaatg ccaccctttc atctgtaaag
     5881 attaacattt tttttcgtta gatacaacag ttttcattca atttcagata aaagtattca
     5941 aattattccg gattcttttt catccttttc ttcacatttt ataacaaacg tgtttatttc
     6001 ctttttcttg tcgctttctc ttctcctgac gtttatcatt ctttcctttg accctctttg
     6061 ttttcatatg aaacgaatta cttgcttccc tttttatttt tagtataata agagtcaaag
     6121 gtcaggaatg gtcaatagag atttcacctt ttctacctca taaatctctt aaaaaactag
     6181 gggttattct tttacctcat gaaaaacgag atgtaaaata tggatgtacc catttttgtg
     6241 aaaggacgtg aatgattcat gctttgtcaa aactgcggca agaatgaggc aacgattcac
     6301 ttgtatgcca ctatcaatgg tcagcggaca caactcgatt actgtcagag ctgttatcaa
     6361 aaaatcaaaa atcaacaaaa tggaggtctg actaatatgg cccaaaatga tccatttgga
     6421 tttggcagtt tggacgatct tttccgttcc atgtctcgcc aaatgcaaca gcaaggaaat
     6481 tatgagcaaa caccaccaac acaatttggc ggaaacaaca acttcaatgg tggtcagcca
     6541 cctcaaggca atacagaagg tttattggga gaatacggaa tcaatatcac agaacaagca
     6601 cgacaaggtg atatcgatcc tgtgatcgga cgcgatgacg aaatcaaacg agtcattgaa
     6661 attttaaatc gccgaaccaa gaacaatcct gtcttgattg gagaaccagg tgttggtaaa
     6721 acagcagttg ttgaaggttt agcacaaaaa atcgtagatg gtgatgtacc gcaaaaatta
     6781 atggacaaag aagtcattcg tttagatgtc gtatctttag tccaaggtac tggtatacga
     6841 ggacaatttg aagaacgtat gcaaaaattg attgaagaga tccgtcaagc agaaaatgtg
     6901 atcctattca tagatgaagt tcacgaaatc gtcggtgctg gttctgctgg tgatggaaat
     6961 atggatgctg gtaatatttt aaaaccagct ttagctcgag gagaattgca aatggttggt
     7021 gctactacct taaatgagta ccgcatcatc gaaaaagacg ctgctttaga acgtcgtatg
     7081 caacctgtac gggtagatga accaactgtc gaagaaacga tcagtatttt gaaaggtttg
     7141 caaaaacgtt atgaagacta tcatcatgta aaatatactg atgaggccat cgaagcagcc
     7201 gcaactttat ccaatcgtta cgtccaagat cgcttcttgc cggataaagc catcgatttg
     7261 ctagatgaaa caggttctaa gaaaaacttg acgatccaaa tcgttgatcc taaaacaatc
     7321 gagaaaaaac tgcaagaagc agaagaacaa aaagttttag cttctcgaga agaagatttc
     7381 gaaaaagcag cttattatcg cgatcagatc aataaacttc aaaaaatgaa agaacgtcag
     7441 cttactgaag aggaaacacc tgtgattacc gaaaaagata tggaaaaaat cgttgaacaa
     7501 cggacaggta ttccagtggg tgaattaaaa gaaaaagaac agacacaact gaaaaactta
     7561 gcagatgact tgaaagctca tgtcatcgga caagataacg ctgtagatcg agtagcgaaa
     7621 gcgattcgcc gaaaccgtgt tggattaaac aaacagaatc gtccaatcgg ctccttctta
     7681 tttgttggac caactggtgt aggtaaaaca gagcttgcga aacaacttgc ctacgaacta
     7741 ttcggttcac aagattcaat gattcgtttc gatatgtctg aatatatgga aaaacatagt
     7801 gtctctaaat tgattggttc tcctccaggt tatgtcggat acgaagaagc tggtcaatta
     7861 acagaaaaag tacggagaaa tccttacagt cttgtcttgt tagatgaagt agaaaaagct
     7921 catccagatg ttttgcatat gttcttgcaa atcttagatg atggtcgatt gacagatgca
     7981 caaggaagaa cggtcagctt taaagatacg atcatcatta tgacaagtaa tgccggaact
     8041 ggtaaagtag aagcaaacgt tggttttggt gcggcacgcg aaggtgttac tcgttctgta
     8101 ttgaaccaat taaacaacta ctttacacca gaatttctga accgttttga cgggatcatc
     8161 gaattcagtg cattatcaaa agaaaacttg atgacaatcg tcacactaat gctagacgat
     8221 gtcaaccaaa tgctggcagc acagcaactt cacatcgaag ttcctacgaa tgtcaaagaa
     8281 aaattagttg acttaggcta tgatccatct atgggtgctc gtcctcttcg acgcacgatc
     8341 caagaacaaa tcgaagatgg tatcgctgaa ttttatcttg atcacccttc tatccatgag
     8401 ttgaaagcaa aactggataa agatggaaaa atcatcgtta cttctaaacc agaacgtttg
     8461 gctaaagaat cagccgaaga aacggctgaa taaatccagt tttatagcgt gagacggtat
     8521 ttctttgaga aatgccgtct cattcttttt tcagaaaagt caaaatactg ttcattttat
     8581 aaaaaagaaa ttataataag agaaacaagc aattaaaaat acataaaaga ggtgagcgcc
     8641 atgaaattgg taaatgtaac aaacagccat tcccgtcttg tattaaacca gcttgaaaac
     8701 actgatgccc atctagtcaa ggtctacaca gccggaaata ccactattgt ctacactgaa
     8761 gcccctgaac ataatgaaat tctgctgatc aatgacaaac gtaaaatcca gccgaaagaa
     8821 atcgaagatg ctaaaaatta tttcttagat aaaatcaaag acgccgttta ccacgaggat
     8881 gaaatcaaag taatcgaatt ggcaggcctt gttgaaatct cgattccaaa acatgaatta
     8941 ggcgctacat tagccgtgta aacaaatttt gaacataata attaaagagc gtgggacaaa
     9001 agtaaagaac acttttgtct cacgctctaa acacgtataa acggcgaaag cagaagcaac
     9061 tccttcggaa ataagctgaa attcacgaaa attgcttttc aaatttttgt gaattccagc
     9121 ttatttcttg gagttaaacg cttctgtctc agcctctttt tctttctagc tttttgattt
     9181 ttctcttttc ttttgtactt ttctcttttc attttcagtt ctacgcgtgt ctttattttt
     9241 aactttttcc tctttatgga tgattgatcg tactttttgt gcaatttgct cgtttttgtc
     9301 ccagtctttt acatctaata atcgacataa tcgtaacatc tcttcttttt tctgtttttt
     9361 tctttcacgt ctttgccatt ctgtttccat gtgtcacact ctcctttctt taatcatatg
     9421 tacgcaaaat aatttttata tttttttgat tagacatttc tatgatttta atatagtaaa
     9481 aagagccgtc acggaaaatc cgtcacggct ctaaacaaaa tatgagaggc tgtttttgtt
     9541 tggctattct tattacttga tgcagggcag cttgtgtcaa gacttcttta catcaaactt
     9601 tttaatttta cattgggata tttatccgca aaccagcgtt ctgcaaattg gttctcgaat
     9661 aagaacaacg gttggtcgaa acggtcacga gccaagatat tgcgacttga actcatcttc
     9721 tcatccaaat cttctggatc gatccagcga gcgattttgt gtcccattgg agacatgatt
     9781 acttcggcgt tatactcatt taacatacga tattggaata cttcgaattg tagctgacca
     9841 acagctccaa tgatatattc ttccgttaga tatgttttat aaagctgaat cgctccttct
     9901 tgaactaatt gatagattcc tttgtggaag gatttttgtt tcatgacatt ttttgcagtc
     9961 actttcataa acagctctgg agtaaatgaa gggagttctt cataagcaac ttttagtttt
    10021 ccttcgaaca atgtatcccc aatctgataa ttgcctgtat catatacacc gatgatatcg
    10081 cctgctactg ctttttcgac gttttctctc gaatctgcca taaattgtgt gacattgctt
    10141 aatttcagtt ttttatttgt acgttccaac cagacatcca ttcctcgttc gaatgttcca
    10201 gaacagattc ggacaaaagc gattcggtca cgatgggctg gattcatatt tgcttggatc
    10261 ttgaatacga aacctgaaaa ttcttcctca taaggactta ccatctgatc atcttgtgtt
    10321 ttgtgtccat aaggagctgg tgccaattca acgaaagtct ctaaaaaagt ctgtacaccg
    10381 aagttagtca atgcagaacc aaaaaatact ggtgtctgat cgccacgagc aattttttct
    10441 tgattaaatg catctccagc ctctttgatt agttctactt cttctaagac ttgttcatat
    10501 aaggaatttt cagttagagg atgattttcg ggaagcagtc catcttcatc caattctact
    10561 aatcgttcat cctgataatt ttccggacga taaaactcta cacgattatg gtagatatca
    10621 taaagacctt ctagcccttt acccataccg attggccaat tcatcggata agattcgata
    10681 tctaatagct cttctaattc ttctaacaat tccaatggtt cacgaccatc acgatctagt
    10741 ttgttgataa aagtaaatat cgggatgcca cgttttttta caacttggaa taatttcttt
    10801 gtctgtgctt cgatcccttt tgcactatcg attaccatga ccgcactatc cacggccatc
    10861 aaagtacgat aagtatcttc agaaaaatct tcatgtcctg gtgtatccaa gatatttact
    10921 cgtttccctt gataatcaaa ctgcataaca gaactagtaa cagaaatccc acgttgtttc
    10981 tcgatttcca tccagtctga tttggcaaaa ttacctgttt ttttgccttt tacggtcccg
    11041 gcttgacgga tagccccacc aaacaatagt aattgttctg taatcgttgt tttcccagca
    11101 tccggatggg aaatgatcgc aaaagttctg cgattgtcca cttgttgctt taattttgga
    11161 ttattcataa cctaactcca tttcttccat tcaattattt atattttctc taggcacaga
    11221 ttgtccgatc tcttaaagaa agacaatttc atatagggct gtgacaaaag ccgtgtagca
    11281 gtctcttttt ccgagtaaac ggcgttctag agacagccta tcagcaataa gcccacccaa
    11341 gccaaaaata cgaaaagctg tttttgcttg gctgctctta ttacttaatg caggatagct
    11401 ttttcacaac ctttgtcaac tactacaact gaatcagtat agcatggatt cagcgaatga
    11461 aaaagccacc aaaccataag agtcaaatat ttttttgtca gctgactaaa agaaaagacg
    11521 tgttgcttgt ttattcaagc aacacgtctt tcataaccaa gtttccaatt atcttcttgg
    11581 ctcgtcatct tcgaattctt ttctgaagta tcttcgttct tcttcaggtt ccgcttctgt
    11641 ttcttccgga tcatgaaaaa tgacacggat tttaaggatt cgagaacctt ccatttcttc
    11701 agaaaccagc gtaatattat cgacatcaaa cgataacttt tcgccttcat ccggaatcat
    11761 gcctaatgct gtaatcaaat aaccagccat cgtatctaca tcactcatat gcagatcact
    11821 gtcaaaagct tcattgaact catcaatcag catacgtcct tggatgatat attcgtgttc
    11881 atcgattttt tcataaagat tctcaacttc atctgattca tcatcgattt ctccgacgat
    11941 ttcttctaaa aggtcttcta gtgttgctaa cccaacgacg ccgccatatt catctaataa
    12001 aatagccatc tgattctgcg tctttttcat ttcatataac agatcatcaa taaagaccgt
    12061 ttcaggaacg aataaaggct cctgcatgat tttcttgata tctaaattat caaagccgaa
    12121 tttatgggca gcttttagta aattctttgt atgaaggatt ccgactactt tatctttgtc
    12181 ttcgttataa acagggattc ttgaatagtt ttcggataaa acttcattca cattttcttc
    12241 cacagtgtcg ttgatatcaa tcataaatgc atccgttcgt gggaccatga cttctcgggc
    12301 aactttcgta tcaagagaaa aaaccccttg gagcatttct aattcttcat tattcaatac
    12361 gccttcgttt tcaagcatat aacgcatttc atctctggtc atttttgaat cttcatcatc
    12421 aaaagtcatc ggcgtgatac gcgccaataa acttgtggaa gcagaaagca gccagacgaa
    12481 gggcttagct actacaccaa tcaagcgtat cagccctgaa gtgaactttg cgacttcttc
    12541 tgatttattt aaagcaatac gtttagggta caattctcca aaaacaatcg atacataggt
    12601 caaaatagcc agcacaatga tatttgcaat attttttgcc gctgtgccgc cacctaatac
    12661 cggagctaac cgttccgata aagtattagc tagtgaagca ccagacaaaa tgttgaccaa
    12721 agtgattcct acttggattg tcgataggaa attatttgga ttctgcaata tttttaaaag
    12781 cttttgagat ttcacatctc cttcatcagc tttttgctcg attcgattct tattgatcga
    12841 aacaacagca atttcggaag ctgctaaaaa agcattgatc aatgtcaaaa cgatcaataa
    12901 taaaatctgc gctaacagcg actgactctc agggtcagca ttcataatcg cattctctcc
    12961 ttataaaatt aatttttagt ttacgataga aaaaaacgtg gcactatagc cacgttaaaa
    13021 tggtatcaca aagcaatttt ctttgcaata aaaacaagtg tactaaaact acctacgagg
    13081 tcgtagcaat tttcttcttg ttttcatttt gcttgtcttc tttgacgatt ttgattatat
    13141 aggaaatgat cgtcttgcag attgcgtaaa aaggaacacc taagaagata ccaagcaatc
    13201 cggcaatatt tccagctact agaagaacga taatgatcgt taatggatgg attttcagtg
    13261 atttcccaat tacattagga taaatgatat tcccatccaa ctgttggacg attagcacaa
    13321 ccacacaaca aagtaatgct tttaccggat cattgaagac tgttactaga aatgcaggtg
    13381 ctagtcctag ataaggtcct aaatatggaa ttaggtttgt caatcccgca atgaccccaa
    13441 ataagaaagc ataatccaca ccaattacca tatatcctaa aaatgtgaag gtaccaacga
    13501 ataaacattc gattgcttgt ccgctaatgt aattagccaa tgttttattc agttgcccca
    13561 ataactccac aatttggttt cttctttttt ctgggaaaaa ctgttggatg tttggcacta
    13621 gtttttctcc gtctttcaac atatagaaca agatgaatgg tgctgtaatg attactacgg
    13681 ttgccgaagc gatcgttcca acgatcgaac ctaaactatt ggatactcca ctaaggaatt
    13741 gctgaatgat tgtcccgtaa gaaatgtcta attgctcaaa atatttattg atatctactt
    13801 ccttgaacaa aggcaattcg gctacctctt ttaaccagct ttctacctgt ttgatgaaat
    13861 caggcatatt agatgccaat gaggaaattt gctgtactag acttgggatg acactaagta
    13921 aaatccaaat aattgctgca actaaaagga gcagcacaat tgcgacagcc catattcttt
    13981 tgatttttgc tttcatcaac agcttgacaa taggattcaa taaatagtaa agaaagcctg
    14041 caatcagtac aggtgcaaat aacgtggaaa aaaatgtccc gatcggttcg aaaataaagt
    14101 ttatttctga tgaaatcatg atcaatgcag ctatgatcaa taattcaagc gaccaaaaca
    14161 ttagtttaga ctgtctaagt ttttcaaaca atagatttcc tcctttcttt cttactagta
    14221 tatattatat tcgttaaaaa ttcttttatt ctttttttat ttttgaaaac ttgccatact
    14281 ttttatttgt tttattttcc agtatactat acgaaaggaa ggtggctaga ccatgaaagt
    14341 tgtacaaaca aaagatacaa tgagcaatat ttacttagat gctgtacgga tcaggcatca
    14401 agtttttgta gtcgaacagg gtgtgccttt gtctcgagaa atagacaaag atgaagccca
    14461 ttgtatccac ttcgtcctgt actcagataa aaaagaaccg caaggaactg tacgtctttt
    14521 gcctttagaa aacggaaaaa tgaaattaca gcggatggcg attctttctg aatacagaca
    14581 ccaagggtta ggaaaaatat tgattgaaga agcagaaaac tttgctaaga atcaaggcta
    14641 taatacgatt ttattagggg cacagtccac agctgaaact ttttacgaaa aactaggata
    14701 tacagcttac ggcgatcctt ttgaagatgc tggcatgcct catatttata tgaaaaagac
    14761 tttataaaaa tttatttcga aaaaagcaaa aaacagcaag cttttgagaa aaatctctca
    14821 ctggcttact gttctttttt gtgtttactt acgcgacaat tgcttgtaac ggttatacca
    14881 aatatcaata tagccttccg agaacgggcc atctccatta ttgatccagt caaccagaat
    14941 cttcacattt tcttttagga tgaagtcaat atcgttcggg taattcatca ttttgctgtg
    15001 catctcatat tcatcgacat ccaatagtcg tttctcgccg tcaggaaata ccttgatatc
    15061 caagtcatag tcgatatatt tcaaggcctc atcgtccagc aaaaaaggtg aagctagatt
    15121 acaataatag gaaaccccct tttctctgat cattgctatt acattgaacc agtatttttt
    15181 atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg cgtccatcag attcagtgac
    15241 taatgtgtga tcattcacgc cgatcataga gtgttcgctt gtttttaata ccatggtatc
    15301 tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt tggatcgtca caaactctcc
    15361 ttcttttgga actcccatag caacccttcc ttaagcagca ttttgtgctt tgtgtttggg
    15421 tgttcattac accgacatca taattattat atcaataaaa atgagctttg cctagaaagg
    15481 attcctattg tttcgatttt tctctaactg atctaccagt ttcatttgga ccttaggaaa
    15541 aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa gttttgttct cggtaaattc
    15601 ttgttctgct ccctcggttg cccgtccgta aaaaaggaga acatgccatt tcagatgact
    15661 aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa acaaagagtt cctgcttttc
    15721 aaatgggaga ttttgctctt cttctgctac taggtcatca aacagatcga tttctggctt
    15781 tgtttcccat tcttttttca atcgctcata ctcctcttgt gtcacttcca tcattggaaa
    15841 tgtccacata ttcgccagca atttttggct atctctttct tcaaaataat acgctccaga
    15901 atgattttga agagctgcac tgatataata aacgtctttt ggctttgctt tttttgtttt
    15961 tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa caaaaggctt ggatcggaca
    16021 agtctcacac tttggagaag ttggtgtaca gatagctgaa cctagatcca tcattgcttg
    16081 gttgaattca cctggatgct tttcatcaat gattttccgc atcgcttcat caaagatttt
    16141 cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac aatctgctga ctactcgcat
    16201 cacgttgccg tcaactgcag gttctggaag tccaaatgcg atacttgcaa tcgcgcctgt
    16261 cgtatatggt cctattccct tcaatgagct aatttcttca ggcgtttgcg gcatttcccc
    16321 atcaaattct gacatgatct gtttagcagc agcttggatg ttccgggctc ttgagtaata
    16381 accaaggccc tcccatgctt ttaaaagctt ttcttctgga gcatttgcta gctcttcaat
    16441 tgtaggaaac cattccatga aacgataaaa ataatcgatc actgtgtcta ctctggtttg
    16501 ctgaagcatg atctcagaga tccagatacg ataaggatca cgattatagc gccaaggcag
    16561 gtttcttttt tcttgttcat accattgaat gaactgatct tgaaattctt ttgtttcttt
    16621 atctgtccat ttttcccatt ctttattcac tttcctcttc cttttccgta atgaatcgct
    16681 ggatcatatc caaatcaaat cctttttgat ataagctttg ctttactttt agatttcttt
    16741 ttttaggttc aaaccttaaa tttcttcgcc atagtttatc tccttgtttg actaaataat
    16801 catattctat ttcttgatct gcttcttgcg gaagatcagc tatcgcttgt tggatcactt
    16861 cttgagaaaa gcctttcgtc atcaatgttt gttggatttt tctcaataat tctttttggc
    16921 tttttccgtg attttttcca aacgcttttt cggctgtctg atgtgcattt tgaacttggt
    16981 cttcaaatgt atattggctg agcgcttgtt caatgatttc aggtttcaaa cctttttgct
    17041 gcatctgctg cgctaatttc tttggtcctt tgtcacttaa gcgcatattc gtgcgcacat
    17101 aactttcccc atagaccaaa tcatcgacaa ggtccaattc ttttaagcgg gctacgattt
    17161 tatttcgatc ttcttgaagg atttcctttt cttttaagta tgttcggatt tctttttccg
    17221 atcgcaactg ataacttaag taattcattg ctaggtgaaa accgaaatca gctccgctac
    17281 tcttcttgat ctcttggata gtttcttcgt ctaattcccg tccttttaat aatcgatagc
    17341 gtactaatac atcttcagat acttgtaaag cttctccttc agataaatct acgcgataga
    17401 aagggccttt gcctttactg attctcgtaa cggttaacat aaacatcctc ctcgaacata
    17461 tattctctat cataccacag aaaatccccg taatcaggta ttatatgtta gaataaacca
    17521 cagaaaaaag aaaaagggag atacttatgg cagaaacaat tgtcaaaact ggacaaacgg
    17581 ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg aatcggttat ttcaaacggt
    17641 tgatcatatt tgttccatat gcactaccaa aagaagaagt actagtgaaa atcacgaaag
    17701 caactcctcg ctatgcagaa ggagaattaa taaaagtcaa aaaaacaagc aaagatcgcg
    17761 tagtggcacc atgtcctgtt tactatgaat gcggtggctg tcaattacaa cacttagctt
    17821 atcaagctca attggatttt aaaaaggatc tgcttttgca agcattggaa aaattcaaac
    17881 cagcaggctt tcgatcttat caacttttgc cgacgatcgg gatggatgag ccttggcatt
    17941 atagaaacaa agcacaattt cagttacgaa aaaataagca gactcaaaaa attgaagctg
    18001 gtttatacga agcaaactct catcagttag tccctataac agactgcttg gttcaagaac
    18061 ctcgaacaca aaaagtaatg aatactgttg ttcaactatt gaataaattt gatgcaccca
    18121 tctatgatga acggacaaac agtggaatct tccgcacaat catggttaga atcggtgtta
    18181 aaactgggga gttacaagtt gtttttatta ctcgcagtaa aaaattcccg caaaaaaacg
    18241 caatgatccg tgagatcaat gaacgactgc ccgaagttgt ttcaatcatg caaaatgtcc
    18301 aggcacaaaa aacgtctctc gtgatggggg atgaaacgat ccacttatgg ggaaaagaag
    18361 ccattgaaga acaaatcaat gaagtgactt tcgatctttc tcctcgtgcc ttctttcaac
    18421 tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat caaagcatta gatgtccagc
    18481 ctaatgaaac gatcgtggat gcttattgcg gagttgggac gatcggctta agtgtagcaa
    18541 aacaagccaa agaagtacgg ggaatggata ccgttcctca agcaatcgct gatgcaaaaa
    18601 aaaatgcgga aagattagga tatcacaaca ctcgatatga agtcgggaca gcagagcaag
    18661 ttctgcctaa atggttgaat gaaggttttc agcctgatgg tatcatcgta gatccaccac
    18721 gtacgggttt ggataacgca ttgatccaat cattattgaa ataccctcca aaaaaacttg
    18781 tttatatctc ctgtaatgtt tctacacttg ccagagatct ggttaattta agtaaaaagt
    18841 tcaagattga atatttacaa tccgtcgaca tgtttcctca gactgcacgt tgtgaagttg
    18901 tcgtgaagat gacgagactc gctgactaaa tgaaagaggt tgtgaaaaag tctgccctgt
    18961 atcaagtaat aagaacagcc aaacaaaaat agctcttcat gttttatgaa tactgtactt
    19021 gtgaccgaag agttaacagg aggacactat ttatttaaaa aaagggttgt gacatagttt
    19081 ttgtcacagc cctttttgat tattattgga tattcacgtc tatcacttga taaaatgcat
    19141 ttgtcgtatc gctgatattc catactgcta aaatcacatt atatcctttt cgatcctgcg
    19201 gaagtgtgat cgtttggttg actgtttttg gtggaacagt tgctttgtcg tctatttttg
    19261 tgatcaattc aaaatcacta aatttcaaag gttgattagg attccaagtt ggtttggtaa
    19321 tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa attccaagta atatttaatg
    19381 ttcctggatt aaccatgttt ttatgccatc tgttagatgt ctgttcatct aaaggctcaa
    19441 agccgctgac tcctgcacta gcaatttttc catcaatgaa tgtatttttg gtagcttcga
    19501 tactttgcgg ttcccattgt gctcttccga cattttgatt taagtttcct gctgctgatg
    19561 ttcctaggta tgcacggctt cctggagaag taatataacc atgcgcaaaa cctggaatgc
    19621 taaaaccgaa caaccctaaa ctaactaacc caataaacaa taacgtacta atctttttca
    19681 tgtagaaacc tccttcattt gataaccaca ttgtaaacgc tttattgtat taacacaaac
    19741 attatgcttt tataatttta tgttgaagta aaaaaggtga aaaaagtaag atgcagatta
    19801 taacagaagt agacgagaaa agtacaaata gaaaacatga gaatcaaatc atatgatctt
    19861 tttcaaaaac atcattcata taaacaaatg ttcaactttt taataacaac aattatcaat
    19921 ttttttctta cttcacaaac taaatacagg tcttgctgtg agccttcatg gtaaactaat
    19981 attatagaaa ggagttagta aaatgaagat gataaaagat attcgttttt gggtttgtgt
    20041 tataggaatt gtcatattag gctttttatc agggttgctt agcggaaatc ccggaaaata
    20101 ttactattct ttgcaacttc caccatttgc accacctagc tggatatttg ggccgatgtg
    20161 gaccttactg tatatattga tgggtatttc tctttatttg ttacttaatc acaacaacaa
    20221 aaaacagcga aacaatcttg tcggtctttt tgtgattcag ttcattttta attttatatg
    20281 gtctgctcta ttttttaatt taagaaatat tttcattgca gcaatcgata ttacactttt
    20341 agttattttc ttgtcagtgc ttatgtatca gttgtggctg catcatcgcc tggcaatgtg
    20401 gctcatgatt ccttattatt tatgggtact attcgcaact ctattgaatt attcaattta
    20461 ttttttaaac taagcgctaa aaagagacgt cctgtaattt aacacataga aaaagtgcca
    20521 cctaagttag attttttgtc taatttaggt ggcactcttt attaaccaaa ctattttttt
    20581 ctagtaccca cttgttgttc catactcatt attctggtca taaccataac cattttgttc
    20641 ctgtccagcg taaggatcat aactttgagt atcttgactt ggtgtatagt actgacttgt
    20701 atcttctgtt tgctgataat aggtattcgg atcttggtac tgatcatatg tgttttctga
    20761 ataggaagaa taatcggtgt acccttcact attcgatcca tcaccgtttg tcgaatcgcc
    20821 atcttccgaa tcatcaaaga acatcattga agcattctca tttttcaaat taggtaatgt
    20881 atcgcttgtt tttatattta attgtttctt cagttcattt tggatcgaaa gaagatcatt
    20941 ttttcctaaa atttgatagt agatatcatt gatcatttgt ccttcgcctt gcaactggtc
    21001 ttgtttgatt gtttcaaacg ccggggtgta gttcgtcgct acatcaagca tgtcatccca
    21061 atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa atttttctat aattgcttac
    21121 gccatctaaa cttaaaactt tattgacgat tttcgtcact acttcccgct gacgacgttg
    21181 tcgaccgata tcgccttctg gatcttcgtg tcgcattcga gaataggcta aacctttttc
    21241 tccattcaat gtttgttcac ctactggtac atggacacca tccaaagtaa agtcaatttt
    21301 attattcact ttgatgccac cgaccgcatt gatcaaatct tccatgccat ccaaattgat
    21361 cgttacataa tgatcaatag gaatatcgag cagttgctca attgaatcca tggccatttc
    21421 gacaccacca aatgcgtagg catgatttaa tttatctacc gttccataac ctacgatatt
    21481 cgtataaata tctcggtcca aactgatgat cgttgatttt ttttgtttcg gattaactgt
    21541 tactaccatc atcgtatcag aacgtccttg ttccgttctt cctaatccac cggtatctaa
    21601 acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg cttactctct cagaacgttt
    21661 ggaggatatt cgattagagt ttttagaaat tttgtttact gtttggctcg cttctcccat
    21721 aaatctgatt ccgtatgctg ataatccggc aatcagcaaa acgaataccc ctaacaaggt
    21781 taatagaact ttttgccatc gcttcataat acacctctgt ttcacatttt ctaaaatatt
    21841 gtctcatata actgttcaat cagcaaattc actttttaat cataagaaaa ctttaaaaaa
    21901 catttttgtg taacgattat tattttttaa aatttctcaa aattcttaac tatcatacca
    21961 taccgtgaaa aagatcgaaa gaccgaatac acgcagttcg caatcgtttt atcttttttt
    22021 atgtattttt tccagaaaaa gcgaacaaca aggtattttc caaataatcg tgcagtaaaa
    22081 tgatcgcaca tacctttccc atctttttaa cagtctttgc ctaccgttca gattatcatt
    22141 cgatttcacg acaatttctt cctatgactg gtccttttgt taaacccatc ttctattcgt
    22201 tttatttcca gctcactgca tggctcttaa aatgactcga actagtgatt gcattaccat
    22261 catcttttaa tggatagata tgctcctctt caaagtaaaa cgattttctt ttaaagtaat
    22321 agaattgaca tggtaatgag tatgtatact agtggtttca caaatggtct tttattcaac
    22381 atccaaaaac tgaaatttct ttaaaatcca ttactaacga agaaaatgtt ccttgcttga
    22441 taattagtta ggacatgtca agtgaacaaa tgcatttact tagccaaaaa atctaaaaac
    22501 ttcataaata gacaaaaaga cacgactctc taagaagtcg tgtctttatt caacaaatta
    22561 ttataattta gtcacttcag cagcttgagg tccgcgtgca ccttcaacaa tcgtgaattc
    22621 tacttcttga ccttcttcta ggctcttgaa gccgtctcct tgaattgctg agaaatgaac
    22681 aaatacgtca tctccgccgt ctactgtaat aaacccaaat cctttttcat tgttgaacca
    22741 tttaaccgtt ccgtgttcca taaatgtata tcctccaaaa aagttcttgc aagtaacctt
    22801 gcaagaacat tatacccaat ttagaaaacg ctagcaagct ttcgctcttt tattttccaa
    22861 attattttta agactgatat gacggaggaa atggttattt tgctgcttcg aaacgtttgt
    22921 ttacttcatc ccaattaata acattccaga atgctgcaat ataatctggt cggacatttt
    22981 tatatttcaa ataataagca tgttcccaaa cgtccaaacc tagaattggt gtctttcctt
    23041 ccatcaatgg agaatcttga tttgcagtag aggtaatagc taattttcca ttttccatta
    23101 caagccaagc ccatccagaa ccaaatcgtc cagcggctgc ttttttgaat tcttctttaa
    23161 aagaagaaaa atcaccaaaa gcttcattaa tcgcttcttt tatttctcct gtaggttcgc
    23221 caccagcatt tggtgccatg atttcccaga aaaatgaatg gttagcatgt ccgccaccat
    23281 tgttacgtac agctgtcttg atatctgttg gaatagcgtc catatcagat aataattctt
    23341 ctattgtttt ttcgcctaat tctgggtatt tctcaattgc tgaatttaaa ttcgtcacat
    23401 aagtattgtg atgcttatca tgatgcagat gcatcgtttc ttcgtcaata taaggttcca
    23461 atgcgtcata tgcataaggt agatctggta atgtgtaagt cataaaaaaa ttcctcctca
    23521 atgttttctt aataatgtct acattacaaa gagtaacaaa gagaaggaaa ttcttcaatt
    23581 gttatgccta gtcgtctttt tcactttgac ctacttcttc ttcaaaaacc tcagtttctt
    23641 taaaaataaa caacttgctg aaaatatagt tcgcaaggat aacaataatt tgagtgatga
    23701 ttttcgccac atactcattg ctgttcataa cattgatcaa caataccatt gcccccatgt
    23761 ccaatccgta agaaaggata cggtagaaga agaacttacc gcattcaata agtgcatgtg
    23821 taaaattcgg tgattttgaa tggaataccc aaagtttgtt cgttacaaaa gcaaacaata
    23881 cagaacaaaa ccacgaaata gaattggata aggccatact taaatcaaaa tattggcgtg
    23941 cgagagcaaa actaacaaaa tttacaatcg tagccaaacc accaaaaaag agatagacta
    24001 gtatctccca atatcctttt gcttctaaat atccttttaa ctgtcgatac tgcttcatga
    24061 gccaacctac cttattaaaa attcttattt atcatactct atatttcaat aactttcatc
    24121 tttatttaaa aaaattaatt ttttttgatc ctttgccttt gtctctgctt ggatcgtcac
    24181 atgtgtaata ccatatttta attttaataa agaaattata cgaggatata tcttttcaat
    24241 ctcgcttagt agctgatctt caaaatcaat atgtccgtcg aaaataatta tcttttcgct
    24301 atactgccaa atatggacat gatgaacatc ctttacccca tcgattgctt caatttcttg
    24361 ttccattttt tcatagtcga tagcaggtgc actttgtatc aaaatagcgg cagctttttt
    24421 gatcaccatt atcgcttcta tcaatatata tatcgaaatg atcagtgtaa taactggatc
    24481 gatccagtag atatcgaaaa aacggatcaa aattgctccg ataatcacac ttaccgaaga
    24541 cagtgtatca cttaacaaat gcaaataact agattttata ttcaaacttt catctgcttc
    24601 ttgtcgtaat aataatactg agaacaaatt cgcgagcaat ccaataactg ctactgttag
    24661 catcaaatga ctatttatct tttctggtga agtgaaccgc ttgaatgctt caacggttaa
    24721 aaacaaggat aatatgatca atatgaatga gttgatgaaa gcactcaata tttctaaacg
    24781 ctggtagcca tacgtacgtt ttttatttgc ttcttttcgg gccatttttt gtgccccgta
    24841 agaaaaaata attgccaatg tatcactaag gttatggaaa gcatctgaaa caagagacaa
    24901 actccctgac ataatccctc caataaattc tgataaagtg atcaccgcat tcaaaaccac
    24961 tactccaaat agtttttttg atgatctctg tggctgtgta ggcactattc cttcctccat
    25021 tttatttata ttggatagtc tttagacaag aataacattt ttttgatatt tcaccaatca
    25081 ttcagctgtt tccttaaagc acttgtattt cacttgatac ttcgctagaa ttgcaagtga
    25141 tgacactact agatgaggag tacttatgac aacaaaacaa ttaattatca gttcctttat
    25201 acgattcaaa gacctaaagg aggcgagaaa tgtcccattt tggaaagcca ttgtctatct
    25261 tttagttttg agtataatta tggctttgcc aatctctttt caagtttttc aagtcttaaa
    25321 caacattcaa ttggatgggc aaaagattgc tactcgcatt cctgatttta caatccaaaa
    25381 cgggcagctt caaaccgaag aaaaagaagg attcatttac caaacaaact ccattatctt
    25441 cacatttgat ccagagggaa aacgaacaga acaggacatc tctactgatt tgatggggaa
    25501 ttttgttagt gtcgggatgt taaaaaacaa acttatcata gctcttccta atacaggcac
    25561 gacttctgct ttgttgaaca ataaccaatt agaacttccc tacaccaatg aatctttaaa
    25621 aaacttaaca ggtaagcagc tccgttcttt tttaagtgaa gcatctatac cactttggat
    25681 aaaagtcctg acatttatat tttctattta tcctagtttt ctgaatctgc tgatcacttt
    25741 gctatttacc aacattgctg cttttctcta tgcacgcttt cgactaacaa aagctacttt
    25801 tcttgattgt ttaaaaacat tgatctattc aatttcattg cctactataa ttgcaacaat
    25861 cttgatggtc tttcttcctt cttttgacac tagcgccttc attgcttttg caggaatctt
    25921 tatttttgct caagctgtca aaggatgggc aaaaatatcg atcaattaag aaataaagac
    25981 aactcgttat ttctacttca atagaccctt ttagaggttg tgacattttt ttgtcacagc
    26041 ccctttttta tttgaaaaat atttctttct caaaaaacaa gcaaggcgct tcaaagttct
    26101 tcttgtatgc gctacactta gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat
    26161 catttatgca gatcgtccat ttcgtgatga atttatcgaa gaaatccata ctatagcgcc
    26221 tggttatatc tttaaaacag aattacaacc agaggatcta caaaatgtag agatcagttt
    26281 gggctggaac tcagactacc aagaaaaact attagcctct tctaccttga aatgggtcca
    26341 atctatctca gctggtgttg atcagctacc ccttgagact ttttcaaaga aacatatttt
    26401 actttcaaat ggtagtggca tccacagtga atcgattacg gagcatgtct taggaatcat
    26461 ccttgggtat tctcgtggtc tttttcaagc acaaaaggca cagagtgaca tgaaatggtt
    26521 aggttcatcg gtccattacc aatctatcca agggaaaaaa atactaatca ttggaactgg
    26581 ccatattgga aaaatgattg caaaaaaatg caaagcatta gacatgaact gctatggaat
    26641 caatacatcc ggtcatccag cagaaggaat ggaaaagact ttttctttaa atcaactaaa
    26701 ggaagttctt cctgaagtag atttcgtagt caatatctta cctctaacgg aggaaacaac
    26761 tgggctcttt gatcagcata tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt
    26821 tggccgtggt gcttctgtta agacacaaga tctggtgcag gctttaaata accagcaact
    26881 ttcctttgca gcattagacg tttttgagga agagcctctt ccacaagatc atcctctttg
    26941 gaaaatggac aatgtactga ttacttccca cattgcaggt ttgacacctg attttcaaaa
    27001 gaaattaatg gctatttttt taaccaatct gaaaagctac tttgccaccc atgagcttca
    27061 aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg aaaacaatat tttcaaaaga
    27121 aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt tttattgttt tttaaagtga
    27181 taggttggct cctccaggtt gggatcataa tcaacaagta aatcatatcc tttaaagaac
    27241 cattcatctg cttcctcgat aaaaaaaagt gtcccatcca tttccacttt ctttacagga
    27301 tgttctggct gttcaactga cattccaatc gaaaaaccat catgaacctc tgttttgccg
    27361 tagacttttc caaaaaaacg tattccgtaa gtttctggca gatcaatttc tgagtcaaac
    27421 cacttttgtg cttttggcgt taccgttaat ttcatcccaa caacctactt tctcttttct
    27481 ttctattgat tcatcttatc gtctagaata tccacatcgc ctgtctctcc aataaccagt
    27541 agattatcat tttcacgaac atattcatct gctgcaggag aagcgataac cgtttgatct
    27601 actcttcgta tggcgactac tgttaagtca aaacgttgcc tgaaatttaa ttcagctaat
    27661 gttttgttga aaaatttagg attggagact cggatttctg ctaaagaata tttatctgac
    27721 aactctaggt aatctaaaat attctttgac actaaattat gcgcaatcct tattcccatg
    27781 tctcgctcgg gatggacgac gcgatcagct ccgatctttt caagtactct ggcatgatac
    27841 tcattttggg cttttgctaa aatatttggt acgcccattt cctttaccat cagagtaact
    27901 aagatactgg cttgaatatc ttctccaata gcgacaatta catgatcgaa atttcttatg
    27961 cctaatgatc gaagtgtcat ttcatcctgt gcatttgcca caacagcatg agtggctata
    28021 ttcatatatt cattgacccg atcttcatta ctatcaatag ccagtacttc ttgaccagct
    28081 tcgatcagtg ttcggcagat acttccgcca aatcgtccaa gacctacaat tgcaaaattt
    28141 tgtttcaccg ttttcctcct ttagtatctt cttttctcag tataacaaag cctttacaaa
    28201 aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa ataatagtta ttttctcagc
    28261 ttttgattat cctctttatc atcgttttta tttcaccaaa ccatgtttga atgcatagat
    28321 agcggcttgt gtacggtctt ccacttctag cttagccaat atattggaaa catgtgtttt
    28381 tactgttttc agtgtaataa acaattcgtc cgctatttcc tgattacttt ttccttcaga
    28441 aatcagcatt aaaatttcat tttcccgatt cgttagttct tcatgtaata caggctcatt
    28501 tcgccgactc attttttcca tcatttttgt tgtcacttct ggttctaata ctcgctcgcc
    28561 acgttgcgtt gcccttatag catcagcaat ttcatgtgcg gtagaggtct tcaacaaata
    28621 ccctgctgca cctgcttcaa tggcaggata aactttttcg tcatcaataa aactggttac
    28681 gataatgatt ttagcttctg gccaatcttt taagattgct tttgttgatt cgataccatc
    28741 catttcttcc atgactaagt ccattaaaat gacgtccggt cgaagtgtca tcgccttctc
    28801 atagccttgt cgtccatttt cagcttctcc aattacttca atgtcttctt gaatagataa
    28861 ataagaagag acgcctaagc gcaccatttc atggtcatct actaataaaa cttttatcat
    28921 ccttatctat gcctccttca tcaaagggac cttgatttct acacttgtac cttgaccttt
    28981 aaaactaatg atttttactg ttccaccaat accagctact cgttctttga tattgttcaa
    29041 tccataactt cccgttttcg tttcattcat attaaaccct gttccgtcgt caataatacg
    29101 taacaaaaga ttattgtcta ttttgtgcaa atatacctct aattcattgg ctttagcatg
    29161 tcttaatgta tttgataaca attcttgaac gattcggaac aagtgatcct caatggaact
    29221 agatagtttt acatcttcta catcccattt cagcgaaatc tggattttat tttgcaattc
    29281 cttcaataac tgctcgatac cttgctttaa acttttctct tctaaattga ctggacgtag
    29341 atgaagaagc agcgcacgca tctcagactg ggaagcgttg atgatctctg ctaccatttt
    29401 caactgcttg cgaaacattt ctggtgtctc gcttttttct gcttgctccg tcaatgccga
    29461 catcatcatc attgctgcaa acaattgttg cgaaactgaa tcatgcagct cacgggctaa
    29521 tcgatgtctt tctaattcta aaatttcttc tttggtttgt ccatctacat attggggacg
    29581 gctcgttaca atttgtaatt cactagatac ttcaatcatt ttttctttta tcttagtgat
    29641 ttccttatca atatctttga tgtataaatc gtccgatgca cttgggatcg gaagaagcaa
    29701 caatttggat tcataattgc cagaagaaag tgcacgcagc ttttcttcga ttttcccgta
    29761 ttttgtcttt tgaataagtg ataataatag aaagacaatt aatcctacac ctaaggaaat
    29821 ggctaacaaa tggaagataa gcggaacata taataaacgt gcacgcagta attctcccca
    29881 ccaatgactt tgattgctgg cataaaaata agtgaaaagt gaaaatagaa taataaggaa
    29941 agccgcaatc cccgaataaa cagctagcat cgctctggat atttttccca tcatattcgt
    30001 atcacctcga catctccaat caaggtatta gtaataatct tcaatctacg agggttttca
    30061 tcataatcac tgctgtagat tttgatttgt tcatttttca aagaaaattg ctcttcttca
    30121 aacaagacat ttcctactaa tgtcgcatgc tctaatcgga tggctacgcc caaaggtact
    30181 aagatccgtg ttctgcctat cccttttctt acaatcacaa tattatcatc ttttggcaaa
    30241 agcgtattgc ccaaatctat gatagtatca ccagaaatga cggcgatgtt gatatcgtcc
    30301 cattcataga cgtcgtttcc aatacgttgg ttaccgaata attgctgtct tttccgctca
    30361 ttattatgtg tttttaattg ctctgtttgg atcatcatga tctgtttctt tcgccaaaaa
    30421 gcatttttgg ttaaatctat ccctgaaatc tctacacctt tcagaccaat aaataaaacg
    30481 gcaaatacag ccatcatcca taaagcagga ctgttgacta agctaaaaaa aatgaccaaa
    30541 cttcctaata tcaattgaaa attttgaaat ttagtacgag gatgtttgcg catagctaga
    30601 tatatattga aaataccgaa tatcactaaa agcaaaagtc cagtattgtt tacgatttgc
    30661 caaacagcaa ataacagcag tagtgcttca actaccacaa aaaaacgcca agaactattc
    30721 atcgctcttt ctccctcctg acacattctc atttcatcat acctattttt ttaactgttg
    30781 gctataggac tttagatgta agatcgtttg ttgaaatcta aaatacacaa agagaaaaag
    30841 atctagagtg tatcatcagt aagatgaact ctctagatct ttcagtcaga ctacgcttgt
    30901 tctactttga cgattttaac taacatatcg ccacctggag tagcaatagt tacttcatca
    30961 tttttacgtt tgccaatcaa tgcttgcgca ataggagaat cattggaaat tttgccagaa
    31021 aacggatctg cttcagcgct tccgacaatc gtgtactctt cttcttctcc atctggtagt
    31081 tcgatgaatg taactgtttt accaattgat acctcatctg catctacacc gtcgttatcg
    31141 atgatttgag caaaacgaat catattttca attgttgtga ttcttccttc aacaaatgct
    31201 tgttcatctt tcgctgattc atattcagag ttttcggaaa gatctccgaa accgcgggca
    31261 attttgattc gttcaatgat ttctccgcgt ttgactgttt ttagttcttc taattctttt
    31321 tctaattttt ctttaccttc tagagtcatt ggataaactt tctctaccat gtgtaacgtc
    31381 tccttttttc gataaaatac cgcaaaaggt aatcttttga ggaatatagt ccaaaaaaga
    31441 ttaccacgct tttcttcaaa tgtaaatgtt tttctaaaaa tgatcaatgt ctagagcctg
    31501 tttttctaag aattatttac atatttttcc actaattcca tatgttcttc ataagtttta
    31561 gcgaaataaa catttccagt gctcgtatct gctacgaaat aataatagtc attttctgct
    31621 ggttctaata ctgccttgat tgcctcttcg cttggattgt caaaaggtcc aggtccatag
    31681 ccggtattgg tatacaaatt atatggtgaa tctacagctg tatcttcata cgtcactaat
    31741 tctttgtgtt cacctaatgc gtataaaaca gagatatctg actgaagtgg catatttgct
    31801 ttgattcggt taaagaagac ttgtgcaatg tttttacgat cattttcttt tacgccttct
    31861 ttttcaacta gagaagctag agttaaaact tcttgtacag tcaaattttt ctgcttgata
    31921 gctgaataat agttttgcat gaccgtattc gtcttgttca ccatttcaat cactagatcc
    31981 ttaagcgaag tactcgtgta ataatcatat gtggctggga ataagtaacc ttccaaacgg
    32041 tattttgtgt tctcagcttt agatgcactt tccaacagtt ctgggaattt ttggtgcaac
    32101 tcgttaaaga aagtctcatc attcactaaa tctaaaaatt ctttcttgtc tttaccagtt
    32161 accttcgcta cccgttcagc aatctgatcg atatcgtacc cttctggaat ggcaattttc
    32221 ccatctgcaa cttttgttgg ctcagaagta ccgccttctt gcaactgttt gccaatctcg
    32281 tcaagagtca tattaggcgc taactgataa tagccagctt gaaagccagt taaattttta
    32341 aattttgtat aataattgaa aacaatcccg cttttaataa tattgtcctt ctccaaaatc
    32401 tcaccaatct gcttgttaga agagccactc gggatctcga cttggactaa ttggtcatct
    32461 gatttatcta aaggcttcaa cccgctatct acataacgat acacggtaaa tccaagaaat
    32521 ccgccaataa tcaataatgt aagagcaatc actaaaatga tcttcctcac aatcttatct
    32581 tcttttttac gcagatttct ccgcttgtcg attatatctg ctgtagctgc cttactggct
    32641 tcttttttat ctttacttgg gtgttgtttg tttcctgaca aaacaactcc tccttactat
    32701 caagttcgta actttattca ttatacatga tatttgaatt ggcgtgaatg attttattta
    32761 aatattctat caaaaatgta agtagtaaat ccattactat aagattaaaa taaatgttat
    32821 caagatacaa gctattttag ctttattctt tctgtaaaaa agaaaggaga ctaaataaac
    32881 tattatgaaa aaaggaaaac atgctaaaaa aagaaaatat tcatttttaa tgatcgttgt
    32941 cgtgttgttt agccttagtg cgttctctac aggaactatt cttggatggc aattttacaa
    33001 taatgaacac tctcagatag ttgcccatgc tcaatcagct caaaaaaagt tgatactgac
    33061 taccaatgaa caacagctcg ttgaaaaaat cgatcaacta ttaagaaaaa atgattatat
    33121 tggctcaatt tacgtaaaga aaaataatcg catcatcatg gaaaaaggct atggctatgc
    33181 gaataaactc acgcaacaac caaatgatcc ttctctttat tatcagattg gttcggttca
    33241 aaaagcattg actactcttt tgattttaaa acaagtcgag cgtgggcacc tttctttaga
    33301 tactaagctg gctcaattct atccacaaat tgctggcagc cagcaaatta ctatccgtga
    33361 cttgctgtat atgcgctcag gcttgcaccg aactgcttct ccaaccatac cgatgactga
    33421 tgaagaagtc gttcaatttg cgattcagca cttaaaattt acagattatc agacctctca
    33481 ttatgattca ttgaacttta ccatattgac tggaattttg atcaaattaa caaatcaacc
    33541 gtatgaaaaa ttattgaccg atgatatcat cgaaccttta ggattacagc atacaaaatt
    33601 ttatgaccaa gtcaaaaatt catccaatca tgcactatcc tatcaaatgt ctaaagagga
    33661 tgattacttt aaaccactga cagaatctga aacagagatc cataacgaat tagggactgg
    33721 taatatcagt atgtccgtct atgatttaaa tacgctgttt acacaagttt tatctggaaa
    33781 actagtttcc aaagacttac tcttctcatt atggcgtgaa aatgcgaatg gacatccata
    33841 tagtggtggg gtatatagcg gagataatta tatcctgtcg caaggaaata tcgactcttt
    33901 tgatacatct gctatattta gcaaagattc acaggatgct atcatcatgg aaagcaatat
    33961 aaaagctgac aaaactatca aactgcctac tgctgatcta agaaaccaaa tctatgacct
    34021 gatggaagga actactaagt tggcaaatag ctgatatgat gaaaagatag caaatactac
    34081 ttctttgaat agcatttgct taaaaaaagt actcgactaa acgtaggaaa gagtgtggga
    34141 caaatctaaa aatcaatttt tgctacagac tttaattcca aataaacaga acagaaagag
    34201 gtgttcttct ttctgttctg tccaaattat tctaagattt ctctttacca acattatttg
    34261 acaaagggcc tattaaatct aaaatagtat ctttagattg ctaaaatatg tgctagcaaa
    34321 tcattcatgg cttgttctta tttttaattt tatgcaataa aaaagaagaa caattagcgt
    34381 gattatagaa ataaataatg agattttaga aaaaaatgtg tatgaatacc tgattgttac
    34441 tttatttatt ccactttcac tttttactgt aacaaaagga cctccttgtt ttagtactgt
    34501 agcatttttt gaattaacag taaccgttaa tcctttatac cggaatacag gtaaaactat
    34561 tgttttgcct tttgaagatt taaacgtaaa tgtaatttca tctttatttg gagaaaagct
    34621 actataaata acttgaccat cttctgtaac ttgatggttt ttcaaaagca attgatcttt
    34681 tttatagcct ataggtacat aatcaaaatt ctcaaattct tcaagtttat tttccatttg
    34741 ttcattagaa taagttcctt gaaaattcat ttctttcacc atgttaacta ctgatgaata
    34801 atttaaaatt gaaatagcta atattaataa tagtataaaa tttcttctcc ttgtataaga
    34861 aaatttttcc aactgtggag aaataaatag agatactgaa aaagatatga acaatgttga
    34921 aaatatcata aaacgccaag gagcttgaat aatatttaaa ggagtatttt gtaaggcaaa
    34981 ccatggaaat atttttgtac aaagtagaat agatattata cccattatat aagtgatttt
    35041 agataagatt cctaattttt taaagttaaa tgttagtata attaaacaaa taagcaaagt
    35101 taagccaata ccaaatgata gtaattctga atctatactt ccaatcaaaa actctttacc
    35161 cagataaatt gctctttttt gtaatatatc cacataaggt ctattcagat cttgatacaa
    35221 tgtctgctca atcataggac ctataaacgc tagtgaaaga agtaaagtaa ctatagcagc
    35281 ttttattaat ttaataatta agaagatttc tattttcttt ttccgataaa attgaataat
    35341 taaagcaatt ataatcatta ccatagtcat agctacagaa agaacatgag tgtaaattaa
    35401 taaagtcatg ctaatcgcca acataaccca agattctttt tttgagtcgt aaagctttat
    35461 aaacccgagt aaaataagag gaagtattga cattgcgagt aattccccca ttgcccctcg
    35521 aagaaatatt tctacactcc taaaagaaga tgttgtatat ataatagcga aaaaggagct
    35581 agtaacatgt tttttggtaa tttcatacat tacataatga catataaata gagtaatgag
    35641 cgtaacaata taaagaaaaa ggttatagcc tattgctaaa ttagtaaact tgatcaataa
    35701 aaataaagga tatagagtta accatgggta aaaattattt acttgcatgc catatgatct
    35761 aaacgtttca aaattaatag gggagctaaa aatagattct aaacttaacg cacgattaat
    35821 gtgaaatgcc gtatcataaa agccaaatat ttccatgttt ttaaatgctg gaaataaata
    35881 taataaacta acaaatgcat aaactataaa tatttttaca gaaaaaattt ttttcaaatt
    35941 catataccac ctcatgtttt tttatattac atatagtatc atttattttt ttaaaacact
    36001 agattagtca ctatataaaa tgtgattaat tttattatta ataaaatatt ttttatatta
    36061 caaaagtagt gtttcaaagt tagtagagaa aatgtagtta tatatttaaa tttttaccag
    36121 aaagaaatga acaccttcaa ttatctaaat ctcgaaaatg gttagatgta atcatcagga
    36181 aaaacaatct taaacgaatt actattcatg gtcttagaca cacacatgct agcttgcttt
    36241 tagcaagcta gtgcaaatat taaagatgtg caagagcgtt taggccactc ttctattcaa
    36301 atcattatgg atctatatat tcatattaca gacaaaagaa aagaagaaac cgtagcgcaa
    36361 ttcgcaaaat ataggctcta taatttatgg gactgatgaa tcaaaattga ttcttcagtc
    36421 ccattgttat tttaggtata tgattcgtac attgaaagag ttgaaaaact atcttccgaa
    36481 gccgtcaaaa aggaccctgc caaaagtatt gatggtggat gaatttcgtt cgcacgcttc
    36541 aattgtggat aaaatgagct ttatttgtgc ggatggcgag actggaaaac tcattgatat
    36601 tttgcctaca cgaaaattgt ctcgattaac acactatttt agtcgctgtg caaatcctga
    36661 agcggtggaa tatttagtga cagatatgaa cgccgcctac ttccaactga caaaacgtgt
    36721 cttaccaaat gcgaaagtcg tcattgattg ttttcatatc atcaaacatt tgaatcaggc
    36781 gttcaatgat tttcgtgtac aagaaatgaa aagattacac cagttagggc aacgacggga
    36841 agccgagaaa gtgaaaaaga actggcgctt tcttctaaaa aaccgtcaaa aaatcaatca
    36901 ctatgattac aaaacatggc gcagttttcg tgcacctaaa tacccgctac tcactgaagc
    36961 aatgatgatt gatcgattgc tggcattttc aacgccttta aaggacgcct atactttttt
    37021 ccatgagctg accgaagcct ttagagacaa ggaatcagac ttatttttta c
//
LOCUS       DACCKU010000020        34522 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.020, whole genome shotgun sequence.
ACCESSION   DACCKU010000020 DACCKU010000000
VERSION     DACCKU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 34522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.020"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(133..306)
                     /locus_tag="IVN13_000926"
     CDS             complement(133..306)
                     /locus_tag="IVN13_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011434.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            754..3116
                     /locus_tag="IVN13_000927"
                     /pseudo
     CDS             754..3116
                     /locus_tag="IVN13_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            3484..4407
                     /gene="truB"
                     /locus_tag="IVN13_000928"
     CDS             3484..4407
                     /gene="truB"
                     /locus_tag="IVN13_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP7011435.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            4411..5352
                     /gene="ribF"
                     /locus_tag="IVN13_000929"
     CDS             4411..5352
                     /gene="ribF"
                     /locus_tag="IVN13_000929"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP7011436.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            5704..6489
                     /locus_tag="IVN13_000930"
     CDS             5704..6489
                     /locus_tag="IVN13_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7011437.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            6502..7353
                     /locus_tag="IVN13_000931"
     CDS             6502..7353
                     /locus_tag="IVN13_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP7011438.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(7477..7929)
                     /locus_tag="IVN13_000932"
     CDS             complement(7477..7929)
                     /locus_tag="IVN13_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011439.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(8394..8762)
                     /locus_tag="IVN13_000933"
     CDS             complement(8394..8762)
                     /locus_tag="IVN13_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP7011440.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            8912..9394
                     /locus_tag="IVN13_000934"
     CDS             8912..9394
                     /locus_tag="IVN13_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP7011441.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            9431..10441
                     /locus_tag="IVN13_000935"
     CDS             9431..10441
                     /locus_tag="IVN13_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP7011442.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(10556..11269)
                     /locus_tag="IVN13_000936"
     CDS             complement(10556..11269)
                     /locus_tag="IVN13_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011443.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(11459..12049)
                     /locus_tag="IVN13_000937"
     CDS             complement(11459..12049)
                     /locus_tag="IVN13_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP7011444.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            12533..12760
                     /locus_tag="IVN13_000938"
     CDS             12533..12760
                     /locus_tag="IVN13_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP7011445.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            12776..13942
                     /locus_tag="IVN13_000939"
     CDS             12776..13942
                     /locus_tag="IVN13_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP7011446.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            14176..15219
                     /gene="hrcA"
                     /locus_tag="IVN13_000940"
     CDS             14176..15219
                     /gene="hrcA"
                     /locus_tag="IVN13_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP7011447.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            15313..15876
                     /gene="grpE"
                     /locus_tag="IVN13_000941"
     CDS             15313..15876
                     /gene="grpE"
                     /locus_tag="IVN13_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP7011448.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            15927..17756
                     /gene="dnaK"
                     /locus_tag="IVN13_000942"
     CDS             15927..17756
                     /gene="dnaK"
                     /locus_tag="IVN13_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP7011449.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVN13_000943"
     CDS             17907..19073
                     /gene="dnaJ"
                     /locus_tag="IVN13_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7011450.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(19155..19820)
                     /locus_tag="IVN13_000944"
     CDS             complement(19155..19820)
                     /locus_tag="IVN13_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP7011451.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            19796..19969
                     /locus_tag="IVN13_000945"
     CDS             19796..19969
                     /locus_tag="IVN13_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7011452.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            complement(20014..20751)
                     /locus_tag="IVN13_000946"
     CDS             complement(20014..20751)
                     /locus_tag="IVN13_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7011453.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(20753..22255)
                     /locus_tag="IVN13_000947"
     CDS             complement(20753..22255)
                     /locus_tag="IVN13_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7011454.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            22514..24508
                     /gene="uvrB"
                     /locus_tag="IVN13_000948"
     CDS             22514..24508
                     /gene="uvrB"
                     /locus_tag="IVN13_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP7011455.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKEELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            24518..27334
                     /gene="uvrA"
                     /locus_tag="IVN13_000949"
     CDS             24518..27334
                     /gene="uvrA"
                     /locus_tag="IVN13_000949"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP7011456.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            27597..28862
                     /locus_tag="IVN13_000950"
     CDS             27597..28862
                     /locus_tag="IVN13_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP7011457.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            28866..29597
                     /locus_tag="IVN13_000951"
     CDS             28866..29597
                     /locus_tag="IVN13_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP7011458.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            29732..30616
                     /gene="rapZ"
                     /locus_tag="IVN13_000952"
     CDS             29732..30616
                     /gene="rapZ"
                     /locus_tag="IVN13_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP7011459.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            30613..31611
                     /locus_tag="IVN13_000953"
     CDS             30613..31611
                     /locus_tag="IVN13_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP7011460.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            31645..32580
                     /gene="whiA"
                     /locus_tag="IVN13_000954"
     CDS             31645..32580
                     /gene="whiA"
                     /locus_tag="IVN13_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP7011461.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            33123..33773
                     /locus_tag="IVN13_000955"
     CDS             33123..33773
                     /locus_tag="IVN13_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011462.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            33960..>34522
                     /locus_tag="IVN13_000956"
     CDS             33960..>34522
                     /locus_tag="IVN13_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP7011463.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
BASE COUNT        11454 a         5832 c         7324 g         9912 t
ORIGIN      
        1 aaatcagggg ttgcgggatt tttttgtttt cattaagacg tattacctaa gagttccatg
       61 actttagtct tgcggcgtgt cagctaacca aaaaagagaa aaagaaaagt aaaaaattcg
      121 actttctttt ttctagtagt atataaattt ttgcatatag ataaagatca agataccgat
      181 aacggaagct agattgtaat ctattttagt gacagtcagg tatagccaag taactagaag
      241 atcagtggaa cctgcaccat aaaagtttga gtttgtcggt ccgttgacag ttaaaagaaa
      301 aatcacgttg aagttattga tatttccgat tacgccgctt cgatttggtc acgatccaaa
      361 ttttggataa tccctgtaga aattagcata gtagctggga taccaattca catattgaca
      421 aagataacag agacttttac ataaaacgga tcaatcagga aaggaattgc tcgatccagc
      481 cccagttttc cagcaatgtg ttaactggcc cagcgtcatt cagcaaatta tgcatgatga
      541 ggagcgaaat gaactgcgga acagccattg tgataacgac aagttttttg gatcaatgta
      601 aaaaacgtca gcgaaaggaa aaatttcgtg gttttttata cctgttcatt tggctagata
      661 taaaatccaa acaaatgaac agaagtgctt attaggtttc aattgggagt atctgcgcta
      721 gaataagagt aataatgaaa aggagtgtta tgtatggatt attctagtaa agaatacttt
      781 gataaaatga cggcttggtg gcgtgctgct aattatcttt ctgttgggca actttatctt
      841 aaagataacc ccttactgag acgtacactg aaacccgaag atgtcaaaaa acatccaatc
      901 ggtcattggg gaacgattcc tggccaaaat tttatttatg tccatttgaa ccgagtcatc
      961 aataaatatg atttgaacat gttttatatt gaagggccag gacacggcgg acaagtcatg
     1021 gtatcgaatg cttatttaga tggaagttac acagagatct atccagaagt aacggaagat
     1081 gagacaggaa tgcaaaaatt attcaaacga ttttcatttc ctggaggcat tgcttctcat
     1141 gcagcaccag aaacaccagg ttctatccat gaaggcggag agcttggata ctctctttca
     1201 catgcagtcg gagctgtatt agacaatcct gaagtaatct ctgctgtcgt gattggtgat
     1261 ggtgaagcag aaaccggtcc tttagctgga agctggttct caaatgtctt tatcaatcca
     1321 gtgatagatg gagctgtttt accgatcctt catttaaatg gtgcaaaaat agccaaccca
     1381 acgatcttgg cacgtaaatc agatggagaa ttagcaaatt actttaacgg acttggctgg
     1441 gaacctttct ttattgaagg aaatgattca gaaaaattaa atcctgttat ggctgaaaaa
     1501 atggatcaag caatcgagaa aattaaatcc atccaaaaag aagctcgcct gaagacagca
     1561 acagatgtcg tgatgccaaa atggccagta ttgatcgtac gcacaccaaa aggatggacc
     1621 ggtccaaaag aatgggacgg ggaaccaatt gaaggaacct tccgagcaca ccaagtaccg
     1681 attcctgtag atcaagaaca tatggatcat gcagatgctt tactccgttg gctgaaatcc
     1741 tatgaaccag aaaaattatt tgatgcacaa ggacgtattt tagaagaaat cagagaaatc
     1801 gcgcctactg gtgaccaaag aatggcaaaa aatcctatca ccaatggtgg aatcgatcca
     1861 agaccgctta ttatgccaga ttggaaaaaa tatactttgc aatttgaaaa accaggctcc
     1921 gttaaagcag aagatatgac cgaacttggt aaattcgttc gcgagatcat tgagaaaaat
     1981 ccagaaaatt tccgtatatt tggaccagat gaaacaaaat caaaccgatt gaatcaagtt
     2041 ttcaaaacga caaatcgcca atggatggaa aaaatcgaac cagaaaatga tgaatggctt
     2101 tcgccatctg gtcgcgtcat cgacagtcaa ttatcggaac accaagatga agggttctta
     2161 gaagggtacg ttctgactgg acggcatggt tttttgcaag ttacgaatca ttcttgcgag
     2221 tggttgactc tatgttgact cagcacttca aatggatgcg gaaatcacat gacctttctt
     2281 ggcgaaatga ttatccttcg ctaaatctca ttgcaagctc aacagtcttc cagcaagatc
     2341 acaatggata tagccaccaa gatccaggaa tattgacaca tttggcagaa aagaaggcgg
     2401 agttcatccg tgaatatctg cctgcggatg caaatacatt gctcgcagtc atggataaag
     2461 cattccgctc gtcagaaaaa atcaatttga tcatctcttc caaacatccg cgtgctcaat
     2521 tttactcagc agaagaagca gccgtcttag tgaatgaagg cttgaaaatc attgattggg
     2581 cttcgacagc aaaagaggag gaacctgaat tggtcattgc tgctgcagga acggaatcta
     2641 acttagaagc attagcagct gtaactttgc tgctggaaga attcccaaaa ctaaaaattc
     2701 ggttcatcaa tgtcgtagat ttattgaaat tacgtcatcc aagtcaagat ccgcgtgggt
     2761 tgagcgatga agaatttgat caatatttta caaaagacaa accgatctta tttgctttcc
     2821 acggttatga aacattggtc cgcacgattt tctttgatcg tcataaccat catttaatga
     2881 ttcatggcta caaagaaaat ggagatatca cgacaccatt tgacatgcgt gtagtaaatg
     2941 aacttgatcg ttatcatttg gcaaaagacg cagctttaaa aatcaaagga agtcaagcag
     3001 aagattttgc gaaaaaaatg gatcagaaat tacaagaaca tcaaaactat attcgtgaaa
     3061 atggtattga tctgccagaa gtattggatt ggaaatggaa aaatcttgac caataaaatg
     3121 ataaaatcga tcccgcaaaa tagaattttt tacattgatt aagaaactga aaaaataaat
     3181 aaaaaaaacg gaaaaaagac tggaagaatg gccagtcttt tttttcgttt ttgggaagtg
     3241 aaaaccaaac tatttacctg taagccaccc ataaatccaa tcaaatggtg caaagaaaaa
     3301 atcttccatt ctattccctc ctttaaaaaa gcataacatg atttctattt acgtgagcat
     3361 gaattttgtt acgtttttat tgccgaaaaa taaaaaccgt tcattatgat gtcaaattcg
     3421 tcagtaattg atgcaatgtg ttataataat tcagttaaat caaaaatgaa atggagaaaa
     3481 gacatggacg ggttattacc tttatggaaa gaacgcggaa tgacaagtca tgattgcgta
     3541 ttcaaattaa gaaaaatctt acatacgaaa aaaatcggac atgggggtac gcttgaccca
     3601 gacgtagacg gtgtgttgcc catttgtatt ggaaaagcca caaaagttat cgaatatttg
     3661 acagattctg ggaaaatcta tgtaggcgag ataactctag gtttcagtac aacgacggaa
     3721 gatcgatccg gagaacgggt agaaacaaaa ccagtagata tgtctttatc tgaaaaaaaa
     3781 atagatgaag tgatggcttc atttattggt gagatcaccc agataccacc aatgtactca
     3841 gcggtaaaag tcaacggtaa gcggttatat gaatatgccc gcaataatga aacagtggag
     3901 cgtcctgtta gaaaggctca gatttaccgt tttatccgga caagtgaatt gacaagagac
     3961 gaagtgacag gaaccatttc ttggcgcttc gaagtagaat gcgggaaagg aacctatgtc
     4021 cgcacattag ctgtagatac aggcaagaaa cttggttacc ctgcgcacat gtctgatctg
     4081 acccgaacag caagtgccgg tatgtcccag aaggatgctc tgacattaga agaagtagct
     4141 caagctgtga aaaatgggac aatcgatgat tgtttgctgc caatagaaaa aggagtggaa
     4201 cggttcaaac gagtagatat tactgatgaa gtatggcaga aagtaaaaaa cggtatgaga
     4261 cttgattata ctgtatttgc gctcccagaa atgccgtcag aagaaattgc tttgttttat
     4321 aaaggaaaag ttgtcagtat ttatcagcca aacccgaaag aaaaaaacaa attaaaacct
     4381 agtaaagtat tgagaaatga ggaataacga atgaatatca tcaagatcag acatccttac
     4441 caatcagaac aaattccaga agacgatgtc gttttagtgc tgggattttt cgacggcgtg
     4501 catatcggac accagaaagt cataaagaca ggtatggaaa ttgcacaaaa agaaggattg
     4561 aaattagcat tgatgacttt caatcagcat ccatctattg tctttaaaaa gatcgatcct
     4621 agcagcgtga aatatttgac tagccttaag caaaaagaag aaaaaatggc cgctttgggt
     4681 atagattatt tatatgaagt tgattttact tcttcttttg ctcatttagc accgcaggaa
     4741 tttgttgatc aatacatcgt agggttacat gcaaaatatg ctgtttccgg ttttgattat
     4801 acgtatggac caaaagacat tgcagatgtc gctcattttc ctgaatatgc caaaggaaga
     4861 ttcaaaatcg tcacagtttc taaagaagaa gaacaaggtg aaaaaatcag ctctacccgt
     4921 attcgtggat tactagacag cggacaagta aaagaagcag caaaactatt aggttgcaca
     4981 tatgaggtag aaggggtagt tgtccatggc gatgcacgag gcagactact aggattccct
     5041 acagcaaatg tcaaagtaaa gagcacggtt catttgccta aagaaggtgt ttatgtgtcg
     5101 gaaataaaaa ttggggaaac atggtatcca gccatgggtt caattggaca taatgataca
     5161 ttcggtgaag gacgggagct gactgtagag ttgtatattt tggactttaa tcaagatatc
     5221 tatggtgaac atgtcgcggt acgctggaat caatttttaa gagatcaagt gaaattttct
     5281 agtgtagagg aattgattga acaactcaag gaggatgaac aagcaacagc tgaatatttt
     5341 tctcagcaat aaaacattat gcataaaatc tcttgtttat gtataactag caattacaat
     5401 aaaatgggtg ataaataagc ccaggaaagg ggctgtgaca aaagtcttgt cacagccttt
     5461 ttttccaaat aaatggtttt tcagaagtag cttcttcgga aataagccga aatttcttga
     5521 agctgaccac ttctgtcaca acctctttct ttatagaaat aaaaggacac tagcaattca
     5581 tgaaaacgat tgctagagcg tatcatttgc tttagactat atatcttggt gtacactcac
     5641 tttaaggcgg aaataaaagt tatccgtaat aacaggttta ggtatgaaaa aggagtgggt
     5701 actatgtaca aggaattaga aaacaaagtc gcagtagtaa ctggtggctc aaaggggatt
     5761 ggaacagcga ttgctcaacg attcgggaaa gaaaaaatga atgtagttgt caactataac
     5821 tcggataaag aaggagcaga aaaagcagtc aaagcaatcg aagaggcagg cggaaaagca
     5881 gtctctgtac aagcagatgt cagttctgaa gaaggaatac agcgtttact tgatacggct
     5941 ttagaaaatt ttgggacact ggacgtttgg gttaataatg ctgggatgga aaatcagcat
     6001 ccaacccatg aacttagcct tgaagattgg gaaagagtgt taaaagtcaa tttgactggc
     6061 gtttttatcg gaacgaaagc ggctttaaat tactttacag agaataataa aaaaggaaat
     6121 atcatcaata tgtcttctgt tcatgaacag atcccatggc caacgtttgc tcattacgct
     6181 gcttcaaaag gcggggtaaa attatttacc gaaacggtag caatggagta tgcaccgaag
     6241 ggaatcagaa tcaatagtat aggccctggt gctatccgta cacctattaa tgcggcaaaa
     6301 tttgatgatc ctgaacaaaa agaactcttg gaaagtatga tcccactagg acgcgtaggt
     6361 gaaccaaaag aaatagcggc gacagctgca tggttggctt cagacgaatc cagttatgtt
     6421 acaggtatta cactatttgc agatggtggg atgactttat atccttcttt ccaaggtgga
     6481 aaaggataag ggtgaataaa aatgggttta ttgattgcat tgatcccggc agttggctgg
     6541 gggatccagc cactgatttt gaaaaagatt ggtggcagac caacaaatga gatattagga
     6601 actggtattg gagcggtgct tgtcggctta ctcgttcaat tattcgcttc tccagctggt
     6661 atttcagtga ctacattttt gattagttta ctttctggtg cattttgggt tattggacaa
     6721 acaggacagt atacatcttt caacttgatt ggtgtttcta aaacgatgcc aatttcgact
     6781 gcattacagt tagtcggaac ctcgttgatt ggtgtcttcg cttttggaga atggtctgga
     6841 agtatgggga aaatcattgg agcagcagca atcattcttc ttgtatttgg ttctgcttta
     6901 acggcaatca gtgatgatcc tggaaaaaaa ggcggtatca caaaaggaat tagtctatta
     6961 gcatcaacaa gtattggtta ttgggtatac agtgcattgc ctaaactagt tgatgcaagc
     7021 ggactttcca ttttctttcc gcaaatgatc ggtgtctttt taggggcagt gatttatgtg
     7081 attttccgta aaccaaccgc attttctgaa gacaagagtt ggaaagcaac gatcgttgga
     7141 gccatcttta gtgtgtcagc attggcttat attttttcag caaatgaaaa tggggttgct
     7201 actgcatata tcattacaca gctaaacgta gttatctcta cattaggcgg tatgtacatc
     7261 ctgcatgaaa ataagacacc aaaagaaaaa aaattaacga ttatcggatt attgctgatc
     7321 gtagtaggaa gcatggtcac ggtctttatt tgaggataaa gatggtaaaa tttcatttat
     7381 taaaaaaata tttgtttgaa ccaaacagta agtacttata aaaaaatagc gctagaattt
     7441 ccaacatctc tggaatttct agcgcttttt aattgtttaa gatacaaggg aaatatctgt
     7501 ctggatcaat aatttttcat tgatccattg actaaagttt acgacataat tgaagttacc
     7561 accgattagt atctccgcat gattgtcttt ataaagatcg tattgtaaaa gacggtcttt
     7621 tttatttgcg taaacatcgt tatggtactg gactaattgt tctcggaatt gattgattgc
     7681 tgtaaaatca cgatattgaa ggttgaaacg catcgtgatt ggtgcattta gcatgaatcg
     7741 ctgcatttct tttgctgctt cctctgcttg atctccgtat aggaactcaa aaagattacg
     7801 ttcagaaaat ttccattgcc cgccaacctt tgttcctttt agttttcctt catgaagata
     7861 atttctgata gaacgtgtag taacacctaa ttgttcggct aattcattta ctgagtaaaa
     7921 atctttcaat gttcaccctc ctatgtgaat ggttatactg acttagttga tatttgctta
     7981 atcacgaaat gtagaaagaa cgattttaat taaggtcatt gtaaaaatca agtcagaaaa
     8041 ttctaacttt tatttctcta aagaaattcg ttctctccta aagtgatatt atatttaaat
     8101 atacctgaaa ataatgggca gtattatgaa agatcactga tattttctcg tattttttcc
     8161 ttattcaaaa gttttgcagg aaaattgctg aaaaaataat tttactaagt agaaaattca
     8221 aagccattca atcagtgatt tttttcagca caagaaagac tccttataaa aatagcagat
     8281 actttgtcaa ttaagtgttt aaccagggat gataaaaagg ataggattga acaaaacctg
     8341 tataaggtga tgaatagttt cctcgccagt aaaaggttta ttcgtataaa gctttatcgc
     8401 caaaatttat catgaccttt tttatcttca atattaaatg aaatcaattg ttcaatcaat
     8461 tcccagttga ttacactttt ccaaggaact ttgatgacgt tatccgtata tacgtagcct
     8521 gcttcttgga tatttttttc aagggcacga atcgctgctt tttccggtgc gatagaaaaa
     8581 tgatttttag caaaactaaa tgcaataata aaggtaccat ggtcagtaaa cataggttgg
     8641 ttccatttta cagttgtgtc tagctgggga tacgtttctg ttatcttagc aaaaaggtct
     8701 ttgatctttt gttgatgttc tttttctttg atttgattga tgatcggctg aaatttttcc
     8761 attctaaaac gcctccatta agtggtttga tttattttac cagaaaaaca atagttatgt
     8821 ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta tatttatatg ataagatgtc
     8881 tcaaataata aaatagaaaa gagacataaa aatgattgac ttggcaaaga aattcttaca
     8941 cgaaaatata tattttgagt tgtatcatca tcgagccatt tatacaaatg aagatgcttt
     9001 ggtaataaag aaagaacaag gatttacagg tacagagacc aaaagcttat tcttaaaaga
     9061 taaaaaagat aataactacg tatatctgac ttttactacg aaacaaacgg atttcaaagc
     9121 attagggaag atcgtcggta aaaggctttc tattgtttca tcagaacgca tggaagaaca
     9181 aacgggacaa aagcagggcg ctgtttctcc gtttggttat gaaaaacaga ttccgattat
     9241 tattgatgaa gagctcttat caaatgaaaa acttgttttt gcaccaggta gaccagatca
     9301 aacgatggtc atccaaacgg ttgacttaaa caaaatcatc cagctacttg agatgccgat
     9361 atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt aaaatagtgc atacttggag
     9421 gtgaagcaac atgataagtt atccattatt agaaagaaat gcaacgattg gggttactgc
     9481 accatcctca ggcgtatcag aggaacacca ttcgttactc gaatcagctt taaagagaat
     9541 ggaagaaaaa tctttccaag tagttactgg aaagacgctt tggacgcaag ataaggaaaa
     9601 atcaggtcca gcaagaaaac gagcagatga attcaatcaa atgatgcaag atgaaaatat
     9661 ccaaatgatc tttccgactt ggggaggaga attattgatt gagattctcg attttgtcga
     9721 ttttgatgct ctacaaccaa aatggatagt aggttattca gatattagta cgttgctact
     9781 tgctgtcaca ttaaaaacag ggatagccac tgcacatgga actaacctta ttgatttaag
     9841 aggagagtac ttggatgaaa cgacagcaat gtgggaaagt gtactgaaaa caagagaagg
     9901 agaatcgatt ctacagcaat cttctgaaaa gtatcaaaaa gtgtggcccc atgagcattc
     9961 atcatcttct gttttccatc tcactgaacc aacaaattgg caatcagtca gcggagatgc
    10021 agtaaggatg aagggacgat tattaggtgg ttgtattgat acgatcaggc atttaatcgg
    10081 tactccttat ggagatgtcc aatcttttaa aagaaagtat aatgatggaa agccgatctt
    10141 gtggtttcta gaaaactgtg agctatcgtc agttgattta agaagatcac ttgttcagat
    10201 gaaactggca ggctggtttg atggcagctc ggggatcatg ttcggtagaa gtgcggcaga
    10261 ggatttgtta gattataaaa tgaaggatgt atacgaagat ctgtcaaagg aattaaaact
    10321 accgatcttg tatgatattg attgtggtca tgtaccgcca caactgacat tgattaatgg
    10381 tgcctatgca gaagtagcgg tagaagatag aaaagcgact gttttgcaaa cgtttattta
    10441 aaaaattttt tcctgccttg gagtctatca aagaccaata aatctaaata gaaaagaaag
    10501 tgaaagaagc tgacaaaatg ctgtcagctt ctttttgtat aacatattag aaagtctaat
    10561 tatctataac tgtcacatcg attaactgtt tcacgtcatt gggatttcct gtaggaatag
    10621 ctactacaga tgcattatca atgatttctt gagttgtttc tctaatgaac gctggataag
    10681 atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg ataactagtg attgtcccgt
    10741 catggttact tgaatacact aaaactccct ggtaactgta tttcccagtg agcataacca
    10801 catctttcgg atagactgca ctccctgcat cataaggggc gataggtgtt cctgcaggat
    10861 aatgaataac atgtaattcg aaatcacccg atgtatctct aaaagtatta cctctaatag
    10921 ctagccaaac acgtgcgtac tcaatttgta gatcagaata tcctgctaac ggatctattg
    10981 actgagaaga ctgtgaggag gttgtgtcta ttgttgaaga agaaatattt gtctcaactg
    11041 tactactaga tgtagaagaa gtattgggct ctgagacttt actgcttgtt gaagatgtat
    11101 tcgtttcagt agaagctgat ttctgagtac ttgatttttc agctgaactg gtttgtctga
    11161 ttgaactttt gtctgtggtc gcagatggat cactattttc tttactagga gacgtacatc
    11221 ctacaaggaa taaagagacc aacaataaaa taaaacctaa ctttttcata taaaatacct
    11281 cattattttt ttaataccag tatagcataa tcctgaattt ttgaatccgt aaaaccttgt
    11341 ctatgaatat aaatagagtg tatcttcttt gataaatgtc agaaaaagtg tgacaaataa
    11401 tcatcagtaa aattacttgt cacactattg ataaggagta tttaattgaa gagaatgatt
    11461 aggagaaagg atctccgtac tttttatagt aatcatagaa tccaagtttg tatagacgaa
    11521 tcgttttttc tcttatttct tctgtttcaa aatggactgc ttgaagaatc aagtctgtga
    11581 attctatagg agctgttcca ttcgcagtaa tcaaattacg atctttcaca gcttgttgtt
    11641 ctataaaatc ttctttattc tgataatttt ccgattctaa ccaaggaaat tgcgcatttc
    11701 cggtgtgttt gtaaccttgc aataatccgc tctgagctaa ataatcaact gccccacaaa
    11761 tagcagctac tggtacgtct gtttttaaca atttatcgat tatttgatat aaatgatgat
    11821 tttttatgtc ccatgaattg ccaccgatca taataaaaag gtcaactttt tcaggtattt
    11881 gttcgattaa gtaatcgatt tttgttttta atcctccaat ggaagtgacc tctgattgga
    11941 tagaagcagt tttgttttgc catttctcat tttggtttag tgtgctagta atataagcac
    12001 cttcccaatc ggcatattcc tctaatagaa acgtaatcgc agttttcatt ttcaagctct
    12061 cctttactat ctataacaag gacaacagtt ttaacctaca gacattcatt aaaattgggt
    12121 agcgacagcg aaaacaacaa ttcttaagtt ttagacagag tatacagata gtcaacttaa
    12181 ttaaatacga actaaataaa aatgacaggt aatcattttt gtttaccagc attataacat
    12241 tttgcataca tggatgacaa aagaaccaat tcccacttgc ttgagtacaa tattaaaaga
    12301 aactggaagg caaaaagtaa ttatagaaac gaaaaataaa tcatatcggg cactcagaca
    12361 tgaaaacaac cctagagata tataactagg taaccagttt aatgaaagaa aaaatcatcc
    12421 aagaagtcga ttcttggatt ttttagtgga tttgttcgcc aaatacgttt agtatgcgta
    12481 tatgagaggt tgtaagcaaa gcgttgtggt ctgagcactt aaggaggaat ctgtgaaaat
    12541 agcttttcat atttttgcag attttgactt aatgccgaag gtcagctttg cgattaccgt
    12601 ttatcgagag gctgtaaaag caccgtgtag ctccgatgac aataagcaag catttttaga
    12661 gctaggtttc cttgattcta aatatgcggc ttatttcaat gagttggtgc ttgaacgcga
    12721 ttttaagaag gtcgaaaaca ggcacgactt ttcgacgtaa ggagaaagaa tttcaatgat
    12781 agaaacaata aatcccgcag aaaacaaggt ttctgcgtac attcatatcc cattttgcga
    12841 gcatatctgc tattactgtg attttaataa agttttttta gaagggcagc cagtagatga
    12901 gtatgtggaa atgctgctga atgaaatgaa aataatgcta gaaagtcatc cgacagcgaa
    12961 tttagaaaca ctttacgtag ggggcgggac accaacttct ttatctgcta aacaacttga
    13021 tcgactgtta tcaggtgcta gagagatcct gccttttcaa aatggtaatg agtttactgt
    13081 agaagcgaat ccaggtgatt tgacccgtga aaaacttcaa gtaatgaaaa actatggcgt
    13141 caatcgttta tccatgggag tgcagacatt tgataatcgc ttattgaaaa aaattggacg
    13201 taaacacacc gcagaagatg tgtatcaaac tatgcaattt ttagaagcag aaaattttac
    13261 caatgtaagc atcgatttga tttatgcatt accgggacaa acactagaag gatatctgga
    13321 tactttggaa aaagctttgg ctttggatct gcctcactat tctttgtact cattgatcct
    13381 cgaaaacaaa acaatgttca tgaactgggt acgacaagga agactcgaac tgcctgatca
    13441 ggaaacggaa accagaatgt ttgaagagac gattgaagca atggaaaagc atggacgtca
    13501 ccaatatgaa ataagtaact ttgggctctc cggacatgaa tctcaacata acctcatgta
    13561 ctggaacaat gaccactatt ttgggtttgg agctggtgct agcggttatc ttggagatat
    13621 tcgttaccgc aacaaaggac caatccaaca ctatttacgc ccattacgag caaatgaacg
    13681 cccggtattg gaagaagaaa aattgacacg aaaaaatcaa atagaagaag aaatgttctt
    13741 aggactaaga aaaaaaacag ggattactaa aaaacatttc tatgatcgct atcaaaaaac
    13801 aattgaatcg gtgtacggtg atgtgctaag ggaattagag caagaagggt tacttgttaa
    13861 tgaagaagac cgtgtttatt tgacaccaag agggaccttt ttggggaaca atgtgtttga
    13921 gcgctttttg tttgaaaaat agtttaaaga atatttgttt tttatctgaa tttaaataaa
    13981 aaaagttcaa aaatacaata aaggactatt gaaacgttga tataactgga tttacatcaa
    14041 ttatttagaa ttagcactct tgtgtttaga gtgctaaaaa atggagtctt tctcttgaca
    14101 ggagaagggc ttcttgctat atttataagt ggattagcac ttagaatata tgagtgctaa
    14161 tgagaggtga ggaagatgtt aacaaagcga caaaacgaca ttttacgtct gatcatccaa
    14221 cactatacga gtagtggcgt gcccgttggt tccaaaacat tgatgtctga gggagtcaag
    14281 gccagctcag caacgattcg caatgatatg aaagcattag aagatgaagg attgctgttg
    14341 aagactcact cttcttctgg gcggattcca tctgctttag gctatcgtta ttacgtggat
    14401 catttgttaa aacctgctcg cgtagcagaa gatgatttgc tgctgattcg tcagtctcta
    14461 ggcagagaat ttcatgaaat caatgagatt attaagcaat ctgctgagat attatctgag
    14521 ctgacaagct atacgacttt ttcattggga cctgaaataa aagaccgccg actgactggc
    14581 tttcgtatcg tacccttgaa taatcgacag attattgcta tcgtcgtaac agatatgggg
    14641 aatgtagaaa gccaagtgtt cactcttccc gaatatatgg gaagtcagga actagaaaaa
    14701 atggtacaga ttgtaaacga ccgtctagtc ggagatcctc tgatgacggt gtaccataaa
    14761 cttcgtacgg agattccgat gattcttcat aaatatttcc aaacgacaga agggatgtct
    14821 cagttatttg acaccgttct tggtcatgtt tttgaagata aggtttatgt cagtggacaa
    14881 atgaatctct tggattatga gccccgtcaa gatcttgatc agttcaaatc gatgttttct
    14941 tttatgaaag actcagacgg acttacacaa atgatcactc cgatggatag caatattcat
    15001 atcaagattg gtgctgaatt aggcaatgat ttattgcaga atatgagtat gattcaagca
    15061 agttacgaga tagatggtca tggaagaggt acaatagcct tgttagggcc tactagtatg
    15121 ccttattctc ggatgttcgg tctagtcgat gtttatcgaa gagaactagc agctaaactt
    15181 gctgattact accgttcgct tgacttgtca aactcgtaat gtgaataacg aagctatttg
    15241 cagctgatcg ttctctcttt cttagtaaga gcaatcaaac agctgactga ataatgaaag
    15301 gaagactgta tcgtgaaaga aaatcaagaa gaactagata aagaaatgac agatgctcag
    15361 ccagaaccgg aaatcgatgt cgaagctgca gaagattcgg gtatttcaga agctgaagcg
    15421 gaagaattcg aaacagcgaa attaaaagct gagttagaag aaatggaaga taaatacttg
    15481 cgggctcgtg ctgaaatcgc aaatatggca aatcgaggaa aaaatgagcg tgagcaatta
    15541 caaaaatacc gttcacaaga tttagccaaa aaattacttc catcaattga caacctggaa
    15601 cgtgcattag ccacagaagt gtcagatgat caaggggcag gtttgaaaaa aggcgtggaa
    15661 atggtcttgg aaagtttgcg taacgcactg gaagaagaag gaattgaaaa aatccctgct
    15721 aaaggagaag cctttgatcc taatcttcat caagcagtac aaactgttcc agcgactgaa
    15781 gatacaccag ccgatacgat tgtagaagta ctacaagaag gctacaaatt acatgatcgc
    15841 gtgttaagac caaccatggt cattgttgca caataattgt gcggatataa aacttacact
    15901 ttcaattaaa gaaggagacg ttaattatga gtaaaattat cggtattgac ttaggtacga
    15961 ctaactctgc agtagcagta ttggaaggaa acgaagcaaa aatcatcgct aacccagaag
    16021 gaaaccgtac aacaccttct gttgtatcat tcaaaaatgg agaaatccaa gtaggggaag
    16081 ttgcaaaacg tcaagctgtg acaaacccaa atacgatttc atcaatcaaa cgccatatcg
    16141 gtgaagcagg ttacaaagta gatgtagaag gtaaatcata tacaccacaa gaaatctcag
    16201 caatgatcct tcaatatatc aaaggattcg cagaagacta tttaggtgaa aaagtcgaaa
    16261 aagcagttat cactgtgcca gcttacttta atgatgcaca acgtcaagca acaaaagatg
    16321 ctggtaagat cgctggtcta gaagtagaac gtatcgtcaa tgaaccaact gcagctgctc
    16381 ttgcctatgg tttagataaa acagatcgcg acgaaaaaat cttagtattc gaccttggtg
    16441 gcggtacttt tgacgtatct atccttgaac taggagacgg tgtctttgat gtattgtcaa
    16501 cagctggcga caaccatcta ggtggggacg actttgataa caaaatcatc gactacatgg
    16561 tagcagaatt caaaaaagaa aacagcattg acttgtctca agacaaaatg gctttacaac
    16621 gcttgaaaga cgctgctgaa aaagcgaaaa aagatttatc tggcgtatca agcacacaaa
    16681 tcagcttgcc atttatcaca gcaggagaat ctggaccatt gcatttggaa atgacattga
    16741 cacgtgcaaa atttgatgaa ttaacagctg acttagtaga acgtacaaaa gtaccagtac
    16801 gtcaagcgct aaaagatgca ggtttatcac aatcagaaat cgacgaagtt attttagtcg
    16861 gtggatctac tcgtatccct gcagttgtag aagcagtacg taaagaaaca ggaaaagaac
    16921 caaacaaatc agttaaccct gatgaagtag ttgctatggg tgctgctatc caaggtggcg
    16981 tgatcactgg tgatgtgaaa gatgttgttt tacttgacgt tacaccattg tcattaggta
    17041 tcgaaacaat gggtggagtg ttcactaaat tgattgaccg caatacaacg attccaacaa
    17101 gtaaatcaca agtattctca actgcggcag ataatcaacc agcagtagac atccatgtat
    17161 tacaaggtga acgcccaatg gctgctgaca acaaaactct aggaagattc caattgacag
    17221 atattccagc agctcctcgt ggtattccac aaatcgaagt aacattcgat attgataaaa
    17281 acgggattgt aaatgtatct gcgaaagatc taggtacaca aaaagaacaa aaaatcacga
    17341 tcaaatcttc ttcaggtcta acagacgaag aaatcgaacg tatggtaaaa gatgcagaag
    17401 caaatgcaga agcagataaa gctcgtaaag aggaagttga cctacgtaat gatgtagatg
    17461 cattgctatt ctctgtggac aaaacattga aagaattaga aggcaaagtg gacgaagaag
    17521 aagtgaaaaa agcagaaaca gctcgcgatg aattgaaagc cgctgttgaa gcaaacaaca
    17581 tcgaagaaat gaaaactaaa cgcgatgcat tgaacgaaat cgttcaaaac ttgactgtta
    17641 aactatacga acaagcggca caacaacaag ctcaagaaaa cccagaagca gctcaaggcg
    17701 gagcagatga tgtcgttgat gctgattttg aagaagtcga tggcgacgat aaataatcag
    17761 ttcactgaat ttcgagagaa ggccaaagtt gctactttgg ctttttcttt tgaaaaatag
    17821 tttgaagtat cggtttttgt ttcaaacaaa atatgatatg ataacaagcg acgtgaaaat
    17881 gatgagaacc aataggagga aagcctatgg caacaaaacg tgattattat gaagtcttag
    17941 ggctctcaaa aggagcatca gaagatgaaa taaagaaagc ttatcgtaag ctttcgaaaa
    18001 aataccatcc tgacatcaat aaagaacctg atgcggaaga aaaattcaaa gaagtatcag
    18061 aagcatatga gattttaagt gacccgcaaa aacgcgctgc atatgaccaa tacgggcatg
    18121 caggcacaga tccaaattac ggtgctggtg gagctggtgg cggatttggt ggattcggcg
    18181 gcttctctgg tggcggcttc ggtggttttg aagatatctt cgactctttc tttggaggag
    18241 gcggtcggtc agtcgatcca aatgcgcctc gtcaaggtgc tgatttgcaa tatacggtcg
    18301 acttgtcttt tgaagaagcg atttttggtg tagaaaaaga aatcaagtat aatcgggaag
    18361 aaatctgtca tacttgtggt gggaacggtg caaaaccagg cacacaacca actacttgtc
    18421 ataaatgtca tggtgcaggt acgattaatg tagaacgcca aacaccactt ggacgagtga
    18481 tgagccgtca aacttgtgat gtttgtcatg ggacagggaa agaaatcaaa gaaccttgtc
    18541 caacctgtca tggctcagga catgagaaga aagcacattc agttaaagtg aatgtaccag
    18601 ctggtgtaga agaaggacaa caaatgcgtt tagctggtca aggagaagct ggagaaaacg
    18661 gcggaccatt tggtgatttg tatgtggtct tccgtgtaga agaaagtgat atctttgatc
    18721 gtgaaggttc tgaaatttac tatgaattgc ctttaagttt tgtacaagca gctttggggg
    18781 atgaagtcca agtaccaact gtccatggag atgtgaaatt gaagatccct gctggaacac
    18841 agacaggtac gaacttccgt ttacgtggaa aaggcgctcc gaagttacgt ggtggtgcaa
    18901 caggcgacca acatgtcaaa gtaaaactga tcacaccgaa aaatctaaat gaacaacaaa
    18961 gagaagcatt acgtgcattc gctgaagctg gcggcatcaa agttgaagaa cagcaagaag
    19021 acggtttctt cgataagttt aaagatgctt ttggcgggaa aaagcggaag tagtttttct
    19081 agtaaaaacg ctaagtcatt aataattaaa aaccagacga acaatcatat gttcgcctgg
    19141 tttttttgta gttgtcatgt ttgtaccaac aaaaaagcca gctttagaat atgataatcg
    19201 tcatagtttc taggggtata accagtgaga gaatggaact tgttcaatcg atattgcaag
    19261 gtgtttttat gaataaataa ttcttctgag caatttttga tgcttccgtt atttttactg
    19321 aagcatgcca aagtcttctt gaacaaatgt acttcttctt ctgtcaggct tttcaaaata
    19381 cgttttacat ataatgttcg ttgatctgac gggattgctg gtaaaacgat cccgaggtct
    19441 aaatcttcaa aaaagcggat ctcgttcaca gattgatgga aagctatcca ttcgcaaact
    19501 tgtttggcat gaaggaaaga ttgcggtgtc tcttgctctg tataacaaag cgacccaatt
    19561 ccaaagttta gctgtaactg atacatttta ttaaactttg tctggatttt atcaagcaat
    19621 gatagcaatg aggctctgga aatccgattg actaggataa tgatttgttg tctatgtaca
    19681 gtgagcaaag cctgtccgta ataagagaag accctttcga gcaactccaa taaaggattg
    19741 atccgttctt gacacaattg ctgattgtca gaaagttgtc ccacaactac ataatatgga
    19801 gaagaaaaat caagctcaat taagtgaggg tggtatcgac tatgtacttg atatcatcaa
    19861 tgaaccgatg gtactttctt atgcgatgga acaggtagag cgattgcttg tttgtgcagg
    19921 aaaacaagcg atgcggctga tacgattagg tgagaaaata agaagataaa aatacacggt
    19981 cccttcatgg cgaccgtgca tttttggttt ttattaaata tttaatactt tattcaagaa
    20041 atcttttgtc cgttcatttt gcggattttc aaagatttgt tgaggcgttc catcttctaa
    20101 aaagtttccg tcgtcaatga acatgacgcg attggccact tctttagcga accccatttc
    20161 gtgcgtaacg ataaccatcg tcataccttg ttctgctaac tttttcatga cgttcaagac
    20221 atcaccgacc atttcaggat cgagtgcaga agtaggctca tcaaaaagca tgatgtcagg
    20281 gttcatcgct agagcacgag caatagccac acgctgtttt tgtccaccag aaagcatttc
    20341 aggatacata tcttttttat cttctagacc aactgtctgc agtaatttga ttgctttttt
    20401 ctctgcttct tctctagatt ctttcttcag gtccatcggt gctaaaatga tgttttccaa
    20461 aatagaaaga tgagggaata ggttaaagtg ttggaatacc attccgatat gttgtctcac
    20521 caagttgata tctgtgcttt tatccatcaa gtgggcgccg tcaataataa tttcaccgtc
    20581 agaaggttct tctaaacgat tcaagcaacg caagaatgta ctttttcctg aacctgaagg
    20641 gccaatgata cagacgacat ctccttcgtt gatggataca ttgatgtcgt ttaatactgt
    20701 gttatcgccg tattttttga ctaaatgctc aactaagatt ttttctgcca tcttacttca
    20761 ccttcttttc taaaactttt gctaattttg tcaatgctgt gatcaaaatc aagtacatga
    20821 tcgcaatgat caagtaaaca tacgtacttt gtgttgtacg tgcaacaatg atcttaccag
    20881 tttgcagcaa ttcgactaca ccgattgcgg aaatgatcgt tgtgtctttt aatgagataa
    20941 cgaactgatt gacgaatgat gggatcatga tgcgtatcgc ttgcggcaaa atgatttttt
    21001 gcattgtccg attataagaa agacctaaac ttctggaagc ttccatttgt ccgacaggaa
    21061 cggcgttgat cccgcctcga acaatctctg cgatataagc acttgcgtta agagtcaagg
    21121 tgataacacc agcaaggaag tcaggaatcg taaatccaac gataccaggc aacccaaaga
    21181 aaataaagaa cgcaagaacc atcattggaa ttcctcggat gatatcgaca taaatactag
    21241 caattgtgcg aagagcgcgt attggtgcta cactgaataa tccaaaaatc acaccgatta
    21301 ctatagccaa agcaaaggag atcaaagtta ggagaattgt tctccaaagt cctttcaaca
    21361 atgcttgata gttgttttgg ataagtccaa gaaatgttga ttcatcagca gtggaagact
    21421 cactggaagt ttctgaacca tccgctacat attctttaat aatcttatcg tattcgcctg
    21481 ttctttttaa ttctttcaat ccttcattga acatattaag aagctcagga ttttgtcctt
    21541 ttttaacggc aaatgcatat tttccgccag attctttttt gatcggtgtt tccaattctt
    21601 gtccttgttt gatcgcataa ccgattaccg gataatcatc catcattgca tcgattgcgc
    21661 caacttttaa cccgtcgtac aattgatcag cagcatcaaa atatttgata gaatagccat
    21721 attcagattc atggtcgctt aaaaattcag cactttcggt accaactttt gctccgactg
    21781 tttttccttt caaatcttca taagacttga tcgaagtatc tccttttttg acagctaatt
    21841 ggatcccgct atcaaagtaa gaatcagaaa aatcatagct tttctttcgt tcatctgtta
    21901 cagtcattcc ggcaatcatt ccgtctgctt gtcctgattc caaagcttgc aaagcagcag
    21961 agaaaccacg gtagtccatt cggatgttaa atccttgcag tttggcgatg ttttccatca
    22021 attttacatc gatgccttcg tactcgccct ttgcgttttg aaattcaaat ggagcaaaag
    22081 cagagtcact ggcaattacg tatacgtctt ttttgggctc tattttttgc attgtctcgc
    22141 ttgtgcttga ctctgccgtt gtagtgttgt cagcttgtgc aacatgacca aacgcaagtg
    22201 tcacaagtcc tgtcattaag aaaacaaatg agtgaaataa atgctttctt ttcataatca
    22261 acctcctaaa tttccaatat tttattatta taggcgaaaa gtgctgaatc agacaagact
    22321 gtaagctcga aaaaatgaaa aaaatgcgag tttctaacat tttatgtcat aaaacataac
    22381 atgtataata agataaaaaa gtggcgaaag tatgttcggt gtggtataat tttttagtaa
    22441 ggaatgcctt tgctttcctt ttctttttat tggtcgtaaa atgaaaagga atgaatgaaa
    22501 aggaggacga agaatgattg agagagatac ctcccgacac tttgagcttg tctcgaaata
    22561 ccaaccggct ggtgatcagc ctgaagcgat taaccaatta gtggatggcg tagtcggcgg
    22621 gaaaaaagca caaatcttat taggtgcgac tggaacaggg aaaacatata cgatttccaa
    22681 tcttattgag aaagtgaata aaccaacact gattattgcc cataacaaaa cattggctgg
    22741 tcagctgtac ggggaattca aggaattttt ccctaacaat gctgttgaat attttgtttc
    22801 ttattatgat tattatcagc ctgaagctta tgtgccttca agcgatacgt atatcgaaaa
    22861 agattcaagt gtaaatgatg agatcgacaa attacgacat tcggctacta gttcattatt
    22921 agaaagaaat gatgtgatcg tcattgcatc cgtttcatgt atttttggat taggctcgcc
    22981 ttttgagtac caaaaacaag tagtttcgat tcgacaggga gcagaactag atcgcaatca
    23041 gttgattcgt gacttagtca gtatccaatt cgaaagaaat gatatagatt ttcagcgcgg
    23101 tcgttttcga gtgcgcggtg atgtcgtgga gatttttccg gcttcaagag atgaacgtgc
    23161 cttacgtgtg gagttttttg gagatgaggt cgaacgtatc cgagaagtca atgcactgac
    23221 aggtgaagtt ttaggagaaa cggaacacgt agcaatcttc ccggcgactc actttgtgac
    23281 gaatgatgaa catatggagc atgccgtagc gaatatcaaa gaggaattag aacagcgttt
    23341 aactgtcttg cgaaatgaaa acaaacttct ggaagctcaa cgtttagaac agcgaaccaa
    23401 ttacgatatt gaaatgatgt tagaaatggg ctacacgtcg ggaattgaaa attattctcg
    23461 gcatatggac ggacgtaaag aaggtgaacc gccttatacg ctattggatt tctttcctga
    23521 agattttttg atcgtggcgg acgaatcgca cgtaaccatg cctcaaatca gaggaatgta
    23581 taatggtgac cgagcgagaa aacagatgct agtagattac ggtttccgtt tgccatctgc
    23641 tttagacaat cggccacttc ggttagaaga gtttgaaaaa catgtcaatc agatcattta
    23701 tgtctctgcg acgccaggac catatgaaca tgaacaaaca gataccgtta tccaacaaat
    23761 catccgtccg acagggctgc ttgacccagt gatcgaagtt cgtccaatca tgggacaaat
    23821 cgatgatcta gtaggcgaaa taaatgaacg agtcgaaaaa gaccagcggg tgtttgtaac
    23881 gacattgacg aaaaaaatgg cagaagacct cacagattat ttcaaagaat tagggatcaa
    23941 agtcaaatat ttgcatagtg atatcaaaac gctggaacga acagaaatca ttcgagatct
    24001 tcgattaggt gaatttgatg tgttagttgg aatcaatttg cttcgtgaag gattagacgt
    24061 acctgaagtt tcccttgttg ctatcttgga tgcggataaa gaaggctttt tacgtagtga
    24121 acgttcctta gtccaaacga ttggacgagc tgctcggaat gaagaaggta aagtaatcat
    24181 gtacgcagac aaagtaacag attctatgcg tctagcgatg gatgaaacgt caagacgtcg
    24241 tacgattcag cagaagtaca atgaagaaca tgggatcgtt ccaaaaacga tcattaaaga
    24301 aatccgagat ttgatttcta ttacgaaaga atcggaagat gatacaaaag aagcggtaca
    24361 agtctcgtac gaagaaatga cgaaggaaga aaaagacacg ttattgatga aattagaaaa
    24421 agaaatgaaa gatgcggcaa aagctttgga ttttgaaaca gccgcgaatg tgcgagatat
    24481 gattttagaa ttaaaagctt caaagtagga ggacaacatg gctaacgata aaattacgat
    24541 tcatggtgca cgtgcccata acttgaaaaa tatagatgta acgattccac gagataaatt
    24601 cgtggtcgtt acaggtttgt ccggttctgg aaaaagttcc ctagcatttg atacgctgta
    24661 cgctgaaggg cagcggagat acgtagagag cctttctgct tatgctcgtc agtttttagg
    24721 acaaatggat aagccggacg tagatagcat cgacggattg agtccggcga tttctattga
    24781 tcagaaaaca acaagtaaaa atcctcggtc gacagtaggt actgtgacgg aaatcaatga
    24841 ctatttacgc ttgttgtttg cacgtgtagg acatccgatt tgtcccaacg atcatatcga
    24901 gattaccagt caatcagtag atcaaatggc agatcaagta atggctttgc ctgagcgcac
    24961 aaagatccaa gtgttggcac cgatcgtggt aaagaaaaaa ggacaacata aaaaaatatt
    25021 cgaacgaatc caaaaagagg gatacgtccg cgttcgagtc gatggtgaga tctatgattt
    25081 aagtgaagtg cctgagctgg agaaaaacaa aaaacatgat atcgcgatcg tcatcgaccg
    25141 gatcattgtg aaagaaggga ttcgttctcg cctatttgat tctttagagg cggccctgcg
    25201 attagcagac ggctatgctc tggttgacgt gatcggtgaa gaagaaatgt tgtttagcga
    25261 acactatgcc tgtccttatt gcggatttac tgttggcgaa ctagaaccaa gactgttctc
    25321 attcaatgcg ccttttggag cttgtccaga ctgtgatggt ttaggtgtca aattagaagt
    25381 cgatcaagat ttagtgattc cagatagaac gaagacactt agtcaaggtg ctatcgttcc
    25441 atggaatcca atcagctctc agtattatcc tcaaatgtta gcgcaggctt gtgaaagttt
    25501 cggtatcgat ttagatacac cattcgaaga attaccggaa gagcatcagg agatcatctt
    25561 gaatgggaca gatgatcttt tccatttcca ttatgaaaat gattttggtg gcgttcgcga
    25621 tgtagaaacg actttcgaag gggtattgaa gaatatcgag cgtcgttatc atgatacgaa
    25681 cagtgatttc acgcgtgagc aaatgcgtct ttatatgaca gaattaactt gtcagacatg
    25741 taaaggttat cgattgaatc cccaagctct atctgttcaa atcaatggaa cgaatatcgg
    25801 gcaaacaaat gagttgtcta ttcaaaaagc agtagatttc ttctcaaact taacgttatc
    25861 tgaacaagag aaagtaattg ccaaaccgat tttaaaagaa gtaaatgaca gactttcatt
    25921 cttggaaaat gtgggactag actatctaac tttgagtcgc gcttccggaa cattatctgg
    25981 gggagaagcg cagcgaatca gattggctac acaaattgga tcgaatcttt ctggagtttt
    26041 gtatatttta gatgaaccat cgatcggatt gcatcaacgc gacaacgatc gactgattgg
    26101 ctcattaaag aaaatgcgtg atcttggcaa caccctcgtt gtagtcgaac atgatgaaga
    26161 tacgatgtgg gcagcagatt atttgattga cgtcggacct ggggccggag aacaaggcgg
    26221 acaaatcgtg gctgcaggca ctcctgaaga agtagctaat gatgaaaatt ctttgacagg
    26281 gaaatatctt tcagggaaaa aatcaatccc tgtaccaaaa gaacgccgta aaggtaatgg
    26341 gaaagcaatc aagatcactg gtgcttcaga aaacaatctg aaaaatatta gtgttgaatt
    26401 tccattagga gaatttgtag cagtcacagg tgtgtctggt tctggaaaga gtacattggt
    26461 caatagtatc ttaaagaaat cgttagcgca aaaattaaat aagaattctg ctaagccagg
    26521 taaattcaag acaatttccg gctacgaaag tatcgaaaag atcatcgata tcgatcaaag
    26581 cccaatcggc cggacgccga gaagtaatcc agcgacttat acaagtgtat ttgatgatat
    26641 ccgtgggtta tttgctcaaa cgaacgaggc aaaaatgcgg ggttataaga aagggcgttt
    26701 tagtttcaac gtaaaaggcg gtcgttgtga agcttgtcgc ggggatggaa ttattaagat
    26761 cgaaatgcac tttttgcctg atgtctatgt tccttgtgaa gtatgtcatg gtaaacgata
    26821 taattctgaa acattagaag tgcattacaa aggaaaaagc attgctgata ttttggaaat
    26881 gacagtagag gatgctgtag aattcttcaa gcacattcca aagattcatc gcaaactgca
    26941 aacgattgtt gatgttggct taggttatgt gacgatgggg caaccagcaa cgacattgtc
    27001 cggtggtgag gcacaacgga tgaaacttgc cagtgaattg cacaaaatct ctaatggaaa
    27061 gaatttctat atactagatg aaccaacgac aggacttcat agcgatgaca tcgcccgctt
    27121 gttgcatgta ttacaaagat tagtagatgc tggtaacaca gttttagtga ttgaacacaa
    27181 tctagatgta atcaaaacag cagattatat cattgattta ggaccagaag gtggagaagg
    27241 tggaggaacg atccttgcga ctggaacacc agaagaaatc attaacgtaa aagaaagtta
    27301 tacaggtcac tatttgaaaa aaataatggt ataaagagga tagatagctg agatagttaa
    27361 aaaaacataa aaaaatgaat ttttgcttgt tttattaata tcagtgttct agaatcatta
    27421 catggtagtt ctttctacac tgattggtat agataaaaac ttcctataag ttcaatccgt
    27481 tcaaaaaaga attgagcaat cggaggactt tcctttgatt gcttttttct ttatggtatg
    27541 atattccttg tgtagttgga aaagaaaaac aagagattat gagtaagtga gagataatga
    27601 tgaacagtat tgaaaatgaa gaatttatcc ctatattatt aggaagcgac atgaacgttt
    27661 atggtatggc acgttctttt aatgaagcat acggcaaaat ctgccaagca tatgcttcgg
    27721 atcagttagc ccctacccgc tacagtaaaa ttgtcaatgt agaagttatt ccaggatttg
    27781 ataaagaccc tgtatttatc gagacgatgc ttcgtttggc aaaagaaaga tattctgata
    27841 agagtaaaaa atatcttttg attgcttgtg gtgatggata tgcagaattg atctcccaac
    27901 ataaacaaga gctgtcagaa tattttatct gtccatatat tgattattct ttatttgaaa
    27961 ggctgatcaa taaagtaagc ttttatgaag tttgtgaaga atatgatctt ccgtatccta
    28021 aaacattgat cgtcagagaa gaaatgttag taaatggaca tttggaacag gaattgcctt
    28081 ttgaattccc agttgctttg aaacctgcaa acagtgtgga gtatctttct gtccaatttg
    28141 aaggaagaaa aaaagctttt attttagaga ctagagaaga gttcgattta attttaggcc
    28201 ggatatacga agccggttat aaaagtgaaa tgattgtaca agattttatc ccgggtgatg
    28261 atagtaatat gcgtgtgcta aatgcgtatg tagatgaaga tcatcaagtt cggatgatgt
    28321 gccttggtca tccactatta gaagatccta caccagcatc gattggtaat tacgttgtga
    28381 tcatgccaga ttataacgaa aaaatctatc aaaccattaa ggcatttttg gaaaaaatcg
    28441 aatatacagg ctttgccaat ttcgatatga agtatgatcc tagagatgga gaatataaat
    28501 tatttgaaat caatttgaga caaggtcgta gcagtttctt tgtgacattg aacggcttga
    28561 atctggctcg ttttgtcaca gaagatcgag tgttcaacaa accctttgtt gaaacgacct
    28621 atggaacgaa tcaatcagat aaagcaagat tatggatggg tgtgcccaaa aaaatctttt
    28681 tagaatatgc tcgggaaaat gaagacaaaa aactagctga gcaaatgatc aaagaaaatc
    28741 gttacggtac tactgtattt tatgaaaaag accgatcaat caagcgctgg ttgttgatga
    28801 aatatatgtt ccataactat attccaagat tcaaaaagta ttttcatgtg aaagaaggct
    28861 agttcatgga gaattttttc agtattttag gcggaatggg cacgatggcg acagaaagtt
    28921 ttgttcgctt gatcaaccac cgaacgaaag ctacaaaaga tcaagaatat ttgaattatg
    28981 tcttattcaa tcatgcaaca gttcctgatc gtacagctta tattttagat cgatcagaag
    29041 aaaatccaat gccgtttctg ctggatgata ttgagaaaca aaatttattg cgaccaaact
    29101 tcattgtgtt gacttgtaac accgcacact acttttttga agagttacaa gcggcaacgg
    29161 atattcctat cttgcatatg ccaagagaag cagcaaatga acttgtccgt cagcatacga
    29221 cagggagagt agcgattcta ggaacagaag gaagtatgaa agctggaatc tatgaacgcg
    29281 aagtcaaaaa tcttggtttc gaaacagtga ttcctgatac tgcgcttcaa gaaaaaatca
    29341 attacctgat ctatcatgag atcaaggaat cggattattt gaatcaagag ctctattacg
    29401 aaatcttaga ggaagcggta gagcgcctaa actgtgaaaa agtcatccta ggttgcacag
    29461 aattatcttt gatgcatgaa tttgctgaag ataatcatta cccagtgatc gatgctcaat
    29521 ctattttagc ggatcgaacg atcgaacgtg cattagctga aagaagcgag gcattggata
    29581 cagcatcgga aaagtaaaaa aattggattt ggggcaaata tcctaaaaga tatttggtcc
    29641 aaatcttttt gtaaaagaaa aagaaaactg gtaatgatac gcccgggatg atataatttt
    29701 attatgcaca atgtgagagg ggttcgtaaa tatgacagat aatttgcaat tagtcatcat
    29761 aacaggtatg agtggtgcag ggaaaacagt agctatccaa agttttgaag atatgggcta
    29821 tttttgtatc gataatatgc cgcctcgatt gattccaaaa ttctgggaat tgatcaaaga
    29881 atcaggtaaa gtcacaaaaa tggctttagg agtggatttg cgttctcgtt ccttctttga
    29941 agaaatccaa aacatgctga tcgaaatcga aaatacgaac ttcatcgaca cttctatctt
    30001 atttttagat gcttcagacg aagaactagt ggctcgctac aaagaaacaa gacgagcaca
    30061 tccaatggcg atggacggtt tgatcactga agggatacgt aaagaacggg cgatactaga
    30121 cgacttgaaa gcccaagctt ccgtgatcat tgatacgaca acattgacac cacgacaatt
    30181 acgtgaaaaa atcaatgagt cattcaaatc aaagggagac caaggatttc ggatcgaatt
    30241 tgtatcattt ggttttaaat acgggttgcc gatcgattcg gatattgtga tggatgtgcg
    30301 ttttttgccc aaccctcatt atatcccgga actaagacca ttgaccggaa aagatcagcc
    30361 agtctatgat tatgtgatgt cctttgatga aacgcaatca ttctaccata aatttttgga
    30421 tcttctggaa actgtattgc ctggatacat taaagaagga aaaagcagct tgacagttgc
    30481 aatcggctgt acaggcggtc aacaccgttc tgtggcattg actgaacgtt tggcaaatgc
    30541 tttagggaaa aactataaag tcaatgtgac acatcgggac aaggataaaa ggaaagagac
    30601 ggtgaaccgg tcgtgaaaat gaaaacctac cggataagaa aaccaaagat cgtagtggtc
    30661 ggcggtggca ctggtctgcc agttatcctg aaaagtttac gaaatcaagg agcagatatc
    30721 acagcagtag tgactgttgc agatgatggt ggaagcagcg gtgcaatcag agaatcgatc
    30781 gctatggctc ctcctggtga cttgagaaat gtcttagtgg cgttatcgga tatgccacaa
    30841 ttctatgaag atattttcca ataccgtttc aaaaaggaag atcagttttt agccaatcat
    30901 acgatcggta atctgatcat tgccgcagtt tccgagatgc ggggaagcac ttatgaggcg
    30961 attcaattat tagcaaaaat gatgcatgta gatggacatg tttatccttc ttcagaaact
    31021 cctttgacct tgcatgctgt ttttaaagac ggaacgactg cagtaggaga atctaaaatc
    31081 gcgatcaacc gtaaaaccat tgaccgtgtg tatgtctgca acacaaatga taaaagtgaa
    31141 gccaaagcgg ctcgaaaagt cgtttcagca attatggacg ccgacatggt cgttttggga
    31201 cccggaagtt tattcaccag tatccttcca aatttagtga taccagaaat cggcgaagca
    31261 atgctcaaga caactgctga aaccgtctat atctgtaata tcatgaccca aaaaggggaa
    31321 acggaacatt tcacagatgc cgatcatata cgtgttttac acaagcattt gaatcagcct
    31381 tttatcgata cagtattggt caatacggaa aaagtgcctg aagattatat ggacccagag
    31441 atctatgatg agtatctagt acaagtcgca cacgacttca acggattgag aaatgaagga
    31501 tgtcgagtga tttccaccga ctttttagaa ttgaaagatg gtggtgtctt ccacgatgga
    31561 gacaaagtag tagaagaatt gttccgttta gttttcggtt caaaatatta agcaatttat
    31621 tgttggagaa aggaggagaa taggatgtca tttgctgcag atgtgaagaa agaactaact
    31681 ggtttggaag ttcatcgaga acatgcgaaa gcggaattag ctgctttgct gcgaatgaac
    31741 ggctctttag gtattgtgaa ccagcagttt attttgaacg tccaaacaga aaatgctgca
    31801 attgccagac gtatctattc tcttttgaaa gatcattatc aggtacgaag tgagctactc
    31861 gttcgtaaaa aaatgaaatt aaaaaagaac aatgtctata tcgtccggtt gaagcaaggc
    31921 acaaaagatg tgttgatgga tcttgatatc atggatggta tgatgttcaa tggacacgtt
    31981 tcggacgaga ttatgggaaa ttcgcaaaaa atgcgttctt acttacgtgg tgcttttatg
    32041 gcctcaggtt cagtcaataa tccggaaacg agtcgttatc atttggaaat attttctatt
    32101 tatgaacagc ataaccaaga catttgcgat atgttaaatt attacggtct acatgctcga
    32161 acgcttgaac ggcgcaatgg ctatatttcg tatttaaaag gagcagaaaa aattgctgat
    32221 ttcttaacat tgattggtgc aaccaactca atgctccgat ttgaagatgt tcgaatcgta
    32281 cgggatatga gaaattcggt caatcgtcta gtcaattgtg agacagcaaa tctgaacaag
    32341 acgattgatg cggcttctaa acaaatcgaa aacatcgaat tgatcgaagc aaaggtcggt
    32401 ctacatgccc ttcctgaaaa acttcaggaa atcgcagaac ttcggcttca acatccagag
    32461 gtcagcttga aagaattagg ggaaatgatt ccctcaggtg caatatcaaa atcgggaatt
    32521 aatcaccgaa ttcgcaaaat aaatgaattt gcggaaaaac tgcgtgaaga agcagtttaa
    32581 tccaaagaaa aaactgatac atgaagcagg agatgcgaat gtatcagttt tttttaaaag
    32641 ctatacttgc ttttcttccg tcaaactata attccaactt tactggagta acataatgtt
    32701 gaaaggcttc tattgttttt taatacaatc gtataaaaag cggggagaca actgaatgaa
    32761 gattttagtt ttatacataa acttttgctt aatatagaag tcctgatata gataggatca
    32821 agtatttatc tataactgga gattaattaa aaactggaag gaactgctcg atcaaccttg
    32881 agaactgttt ttcttacttc tggtcaagat tctcgtggtt ggtggataag gatggttgat
    32941 aagttcattg gattagctag tcgaaaacaa ccaggtgtaa ttaatgtaga agttttaaca
    33001 aggggatgga gtgatttatt cagtgataat cataagttca taaatagtaa ttgggatgta
    33061 tggttggtat acgttatacc tgaacttgaa ctatcaagat ttggtttaga atttgagaat
    33121 cgatgtttat aaagggaggg caaaaaatgt gcttttttac aacttttact atcattatga
    33181 ttgcagtact ttttattttt ctagatatag caaagcgaaa tacagcattt ttattatatc
    33241 gggttttact tcgagcaggt ctcataacat ttatatcgat cgttggattt tttctattca
    33301 cagttattgt atttatttgg agaactacag caccaccatt gccagagatt acatatggag
    33361 aatttccgtt tagtcttgaa tatgaaatag atgaggaaag aattttgatt gaagatacgg
    33421 tgattgcaga atttatagga agttttagag ggaatgtgac aactcaacct agacgaagtt
    33481 ggagtataaa acttttaaat aatagtggag agttgaaagc atttcattat gaattcttaa
    33541 taatagaaag tgaagatgta acaatcactt taagtagtgg ctctgctgga tattttatga
    33601 atgatggtgg aagcaagcca cagctcacag tccgtgatcg tattgcaggt tttatgcatt
    33661 tttctcacta tgacttagaa gaagctcgta aaatacttgc agaatatggg atcacattaa
    33721 tcaattggga atatgcgcca ccgataagaa attttttcaa tgattattat tagtcttata
    33781 aaaaacagaa tgtaaaattt atgtgcgcga gtatttagtt ttccattttt tgtttgatag
    33841 atatatagca ttgttgaagc atgggatagt ggtgccaatt ttatcaatga ggttggtcag
    33901 gcagttaaca taattggtga tgctataaat ccatttaatt ggaattggag gaattaggtg
    33961 tggaaacagt gagattatgg atattcatga aaaattttcg ttcggctttg agatatgtta
    34021 ttctggaatc aaaatcaggt tattatctga ttgacacaga tcactatact ttttggggag
    34081 gctacctttt tccgattttt aattggataa tcccccataa ggttatgaaa attaacttgc
    34141 ctgcagaaga aataaataga ttaatactca acaaagattt acaaaggaaa agagagaaag
    34201 aacattctaa tgttgcttgg ataggtccag catttgcagt agtgacttct tcaattgtcg
    34261 gtcctatgct agagagtatt tatagtgatt tttctattgg aatacgttat ttatttgtta
    34321 ctttaagtat agctgttctt ttgtgcataa agggttatgt tagcaagaag aataaagata
    34381 ttttaacact aatcggtgta aacaatttat ctagtactaa aattatactg tttcctgaat
    34441 ctataaaaga aggatcaagg ttgttgataa tttttataat gcttatactg ttttctgttt
    34501 tatttggggt gatgtttata ga
//
LOCUS       DACCKU010000021        33775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.021, whole genome shotgun sequence.
ACCESSION   DACCKU010000021 DACCKU010000000
VERSION     DACCKU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 33775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.021"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            211..1080
                     /locus_tag="IVN13_000957"
     CDS             211..1080
                     /locus_tag="IVN13_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP7011464.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="IVN13_000958"
     CDS             1289..2314
                     /locus_tag="IVN13_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP7011465.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="IVN13_000959"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="IVN13_000959"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP7011466.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3397..5100
                     /locus_tag="IVN13_000960"
     CDS             3397..5100
                     /locus_tag="IVN13_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7011467.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="IVN13_000961"
     CDS             5102..7135
                     /locus_tag="IVN13_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7011468.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="IVN13_000962"
     CDS             7146..7586
                     /locus_tag="IVN13_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7011469.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7786..8913)
                     /locus_tag="IVN13_000963"
     CDS             complement(7786..8913)
                     /locus_tag="IVN13_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011470.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8916..9593)
                     /locus_tag="IVN13_000964"
     CDS             complement(8916..9593)
                     /locus_tag="IVN13_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011471.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9857..11623
                     /locus_tag="IVN13_000965"
     CDS             9857..11623
                     /locus_tag="IVN13_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP7011472.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11638..12075
                     /locus_tag="IVN13_000966"
     CDS             11638..12075
                     /locus_tag="IVN13_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7011473.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12088..13239
                     /locus_tag="IVN13_000967"
     CDS             12088..13239
                     /locus_tag="IVN13_000967"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP7011474.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13301..13999)
                     /locus_tag="IVN13_000968"
     CDS             complement(13301..13999)
                     /locus_tag="IVN13_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP7011475.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13992..15344)
                     /locus_tag="IVN13_000969"
     CDS             complement(13992..15344)
                     /locus_tag="IVN13_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP7011476.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15567..16967)
                     /locus_tag="IVN13_000970"
     CDS             complement(15567..16967)
                     /locus_tag="IVN13_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP7011477.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17128..17865
                     /locus_tag="IVN13_000971"
     CDS             17128..17865
                     /locus_tag="IVN13_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP7011478.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17990..18469
                     /locus_tag="IVN13_000972"
     CDS             17990..18469
                     /locus_tag="IVN13_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP7011479.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18466..19233
                     /locus_tag="IVN13_000973"
     CDS             18466..19233
                     /locus_tag="IVN13_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011480.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19463..20032
                     /gene="lepB"
                     /locus_tag="IVN13_000974"
     CDS             19463..20032
                     /gene="lepB"
                     /locus_tag="IVN13_000974"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP7011481.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20136..20738)
                     /locus_tag="IVN13_000975"
     CDS             complement(20136..20738)
                     /locus_tag="IVN13_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7011482.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20844..21313
                     /locus_tag="IVN13_000976"
                     /pseudo
     CDS             20844..21313
                     /locus_tag="IVN13_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21419..22003
                     /locus_tag="IVN13_000977"
     CDS             21419..22003
                     /locus_tag="IVN13_000977"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP7011483.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22006..23079
                     /gene="prfA"
                     /locus_tag="IVN13_000978"
     CDS             22006..23079
                     /gene="prfA"
                     /locus_tag="IVN13_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP7011484.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23072..23911
                     /gene="prmC"
                     /locus_tag="IVN13_000979"
     CDS             23072..23911
                     /gene="prmC"
                     /locus_tag="IVN13_000979"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP7011485.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23904..24083
                     /locus_tag="IVN13_000980"
     CDS             23904..24083
                     /locus_tag="IVN13_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011486.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24105..25130
                     /locus_tag="IVN13_000981"
     CDS             24105..25130
                     /locus_tag="IVN13_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP7011487.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25305..26549
                     /locus_tag="IVN13_000982"
     CDS             25305..26549
                     /locus_tag="IVN13_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP7011488.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26702..27649
                     /locus_tag="IVN13_000983"
     CDS             26702..27649
                     /locus_tag="IVN13_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7011489.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27783..28412
                     /gene="upp"
                     /locus_tag="IVN13_000984"
     CDS             27783..28412
                     /gene="upp"
                     /locus_tag="IVN13_000984"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP7011490.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28558..28794)
                     /locus_tag="IVN13_000985"
                     /pseudo
     CDS             complement(28558..28794)
                     /locus_tag="IVN13_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28915..28987
                     /locus_tag="IVN13_000986"
     tRNA            28915..28987
                     /locus_tag="IVN13_000986"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28948..28950,aa:Thr,seq:cgt)
     gene            complement(29106..29744)
                     /locus_tag="IVN13_000987"
     CDS             complement(29106..29744)
                     /locus_tag="IVN13_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7011491.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29867..30499)
                     /locus_tag="IVN13_000988"
     CDS             complement(29867..30499)
                     /locus_tag="IVN13_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP7011492.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30850..31149
                     /locus_tag="IVN13_000989"
     CDS             30850..31149
                     /locus_tag="IVN13_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7011493.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            31644..33775
                     /gene="acm"
                     /locus_tag="IVN13_000990"
                     /pseudo
     CDS             31644..33775
                     /gene="acm"
                     /locus_tag="IVN13_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
BASE COUNT        11653 a         5824 c         6773 g         9525 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8641 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8701 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8761 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8821 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8881 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     8941 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9001 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9061 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9121 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9181 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9241 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9301 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9361 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9421 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9481 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9541 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9601 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9661 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9721 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9781 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9841 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9901 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
     9961 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10021 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10081 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10141 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10201 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10261 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10321 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10381 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10441 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10501 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10561 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10621 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10681 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10741 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10801 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10861 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10921 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    10981 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11041 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11101 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11161 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11221 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11281 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11341 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11401 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11461 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11521 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11581 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11641 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11701 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11761 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11821 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11881 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    11941 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12001 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12061 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12121 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12181 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12241 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12301 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12361 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12421 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12481 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12541 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12601 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12661 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12721 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12781 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12841 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12901 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    12961 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13021 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13081 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13141 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13201 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13261 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13321 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13381 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13441 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13501 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13561 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13621 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13681 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13741 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13801 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13861 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13921 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    13981 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14041 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14101 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14161 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14221 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14281 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14341 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14401 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14461 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14521 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14581 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14641 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14701 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14761 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14821 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14881 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    14941 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15001 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15061 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15121 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15181 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15241 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15301 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15361 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15421 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15481 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15541 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15601 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15661 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15721 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15781 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15841 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15901 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    15961 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16021 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16081 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16141 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16201 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16261 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16321 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16381 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16441 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16501 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16561 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16621 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16681 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16741 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16801 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16861 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16921 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    16981 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17041 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17101 gaaaaaagaa ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg
    17161 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17221 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca
    17281 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17341 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17401 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17461 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17521 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17581 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17641 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17701 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17761 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17821 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17881 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    17941 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18001 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18061 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18121 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18181 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18241 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18301 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18361 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18421 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18481 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18541 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18601 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18661 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18721 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18781 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18841 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18901 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    18961 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19021 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19081 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19141 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19201 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19261 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19321 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19381 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19441 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19501 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19561 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19621 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19681 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19741 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19801 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19861 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19921 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    19981 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20041 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20101 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20161 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20221 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20281 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20341 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20401 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20461 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20521 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20581 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20641 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20701 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20761 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20821 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20881 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    20941 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21001 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21061 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21121 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21181 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21241 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21301 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21361 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21421 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21481 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21541 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21601 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21661 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21721 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21781 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21841 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21901 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    21961 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22021 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22081 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22141 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22201 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22261 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22321 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22381 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22441 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22501 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22561 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22621 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22681 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22741 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22801 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22861 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22921 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    22981 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23041 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23101 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23161 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23221 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23281 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23341 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23401 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23461 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23521 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23581 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23641 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23701 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23761 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23821 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23881 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    23941 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24001 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24061 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24121 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24181 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24241 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24301 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24361 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24421 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24481 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24541 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24601 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24661 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24721 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24781 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24841 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24901 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    24961 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25021 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25081 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25141 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25201 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25261 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25321 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25381 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25441 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25501 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25561 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25621 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25681 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25741 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25801 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25861 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25921 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    25981 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26041 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26101 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26161 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26221 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26281 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26341 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26401 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26461 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26521 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26581 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26641 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26701 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26761 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26821 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26881 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    26941 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27001 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27061 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27121 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27181 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27241 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27301 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27361 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27421 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27481 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27541 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27601 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27661 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27721 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27781 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27841 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27901 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    27961 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28021 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28081 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28141 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28201 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28261 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28321 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28381 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28441 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28501 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28561 ttttagttct ttgtagttaa cgattactac acccttactt tgcgtcatca agttcgcatt
    28621 ttctgcagtt tcatcaatct caacaacagc ggaatttttc aattgctttg aaatcgttcc
    28681 agtttgcgac ttgccgtgaa ttgtgaacat tacgtgagta ttgatcttga atttttcacc
    28741 aatttccgat ggtacgatag ttgtatcaaa ttttccaaaa gttgccattt tcataaaacc
    28801 tgcctttcac atcaattata ccacgtttta tgaaaaatat catgaaatta aactaatttt
    28861 ccatcggact ctttacaatc agccaaatag agtatataat ttcttttgga taaggccagc
    28921 ttagctcagt tggtagagca acgcactcgt aacgcgtagg tcgtaggttc gatccctata
    28981 gctggcatta aaagagaaga cagatatatc aaggattttt aatccattga tacatctgtc
    29041 tttttgttta aaggaaataa agtatgcttt cttagcaata gagcatgtat tatttctata
    29101 aaaatttagg agaataaaac gactagcaat atagctgtga aatcaaatat cacatgaact
    29161 gcccaacttg taatgatcga gttgctttta atggcagctt ggttgaataa caatcttgcc
    29221 accccttgta tcaaaaggat gtgtgctaca gtgtgtaaaa tatttccatt atcataagta
    29281 ctgtaatgga taaatccaaa gatgattgct gacaagaaac tagcgacagg aatgctgaac
    29341 ttactagaaa aaatagcgag aaaatacact gagaacaatt cttctccaag tatcatgata
    29401 ggcaaaataa taaataataa cgggctgaaa gcctcattta ttggattgcc tgttaaaccc
    29461 catttcaaaa tttgttgcaa aaataccgag actgcggcac tgattactac attgataaaa
    29521 aaatatttgg ctatattttt gacaggagcg acaggtttcg agaacatttt tttcgtatct
    29581 tttttaccaa aaacaagtgt aaatgctata aatccagtga tagttaaaga aaaaatgcta
    29641 ataatggatg gcgcaaatag caaccctaac aaaccgagta caacgacaat agtgcccaat
    29701 atcccttttt taagttctat tttttttcct aaaatgttat tcatgataat aactcctact
    29761 attcagtttt tgaagataga gtcagtatac atgaagagtt aaaataggca atgttttttt
    29821 atggatattt gtattgctaa aaatttatct tatatattca ttttgattat actactttta
    29881 tttgtaaaaa ttttctagca aaaccaagtg cttgcaactg attaagcgta caatttttta
    29941 gatgttccga gcgtacttca atcatttcaa aggagttgct acttaaatcg atatttttta
    30001 aatcagtatc aaaaaagcta gcattttcta aatcattagt agaaaacatt attttagtga
    30061 gggaacagtc tacaaaatct gcgcttgata agacagagtg ctcaaaggta ctttctttta
    30121 tgtttgaacc agataaattt acatagtcca ttttacaatt ctcgaataac gtgttcccaa
    30181 tataactttc catcaattta gtacctgtta atttacaatt gacaaatttc acgcgatgca
    30241 acatgctttt tgtcaatata cagcctgaaa aatcacagtt ttcgaatata cagtcggtaa
    30301 tctccattct ttgaaaatca ttggaagcaa aaaatgcata cttgaaaata gatttgtaaa
    30361 taattggatg gcagattatt tggttttcga tgacctcttt ttgtgaaagg atattataca
    30421 gttcaaaatc ttcgttttca tagatatgaa aaaaggatcc tttattcaag cttgcttcat
    30481 taactttagg aaaaatcata tttaattacc tccattttca taaagtcata ttaacaaaag
    30541 ctaaaccaac ttacattagc agaaaatgag ataggttttc gcctttttcg ataacgatag
    30601 tgtcgctatc tcttgaaacg ttcttagaat tgccctgaga gacacaaatt aggcgtaatt
    30661 ctaataaaaa tcttagaata aaaattttta ctgatttaat ttagaatgtt tttaaaataa
    30721 aaatcgttgc cataacagtg tattgaaagg ttttaacaat ccagtaatga tttaaaaaaa
    30781 tctagatgta gcgtatactt tgtttataga atgtagtggg aagaaatata tttttgggag
    30841 gaaatcgtga tgtctaaaag tgaagaaata gaaaacatgg tactaaaatt atatgatctc
    30901 gttttaaatc cagagatcaa agaaaaggaa cgtatacttt tgatagacgc aaagacaggc
    30961 ttagaaaaag gtcagtatta tcctaaagtc ataaataatt tggaacgtag tttacgtccg
    31021 ttagcaatca ggggagaatt gtctaaacca gtatcaccgt tttatatgga gatatcaaca
    31081 ataggaaaat ttgaaaaaga attaggtcga ggaatggctt cggcaccgat tacttttggg
    31141 catttataaa aacgagttat tcatctcaat gaattaaaaa gtatagtcaa tctcataatg
    31201 caggctaggt tcatttattt gaacagagtc tgtatttttt tattctaaat aaagaaacag
    31261 atgtttcttt atattgtatt ttatccttta attaacacaa aaacagctag tataaactaa
    31321 taatgttaat taaaagtgta ttttatgaaa ctatcgttat ttattaatgt tgcatttttg
    31381 taagttaaaa attggaaata tagattgaaa aaatgaaagc aataagtaaa ataaaatgaa
    31441 tatatgcgaa aaaataattt acattaggaa aaaccttgta aaaacagttt tttggcatat
    31501 ttatttttta taaatatttg taaaataact atcaattttt tagttaaaag attggaaatg
    31561 agtgggggag gaattgaaga aaggattttt gagagatgat atagtaggtt ttatttttag
    31621 ttaaaatagg ggagatagaa aaaatgaaaa agtgtagaaa tattatttta tcaatgttat
    31681 ttattattac taatataaca tcactgattc ctgttcatgt ctatgcggat gcaggcagag
    31741 atatcagcag taatgtcact tcgttgaccg tggacccaac gaatattaca gatggaggaa
    31801 acataaaagt aaaattctcg tttgacgaaa aaaaacaaaa tatccaacca ggtgattatc
    31861 tttggatcaa ttggccaagt gaaggaaata ttcgcggtga aggattccaa aaagaaatcc
    31921 cattaatgat tgaaaataaa aatgttggaa ctttaacggt cagaaaagat tctgcgcagg
    31981 ttgtgtttaa tgaaaatatt aaaaacctag actcagtaga aggttggggg caatttgaga
    32041 tacaggccag aaacgtaacc gataccggtg aagaaaatac gggaccattc accgtgacga
    32101 gcggtgataa aacagctact gtaaatgtga caaagccagc ttctggttct tcttctagcg
    32161 tttttttatt ataaaacagg agacatgcta cctgaagata ctaagcacat tcgatggttt
    32221 ttgaatatta acaataacgg cacttatgtt gaacaaccag ttaaaatcag tgatgaaatc
    32281 caaagtggac aaagattaga tccaagtacg tttgaaatta atcaaataca cttaggtgaa
    32341 cagaaggttt atagaggaga agagggaatc caacaatttt tacaagattt ccccagcgct
    32401 acatttaatt tttcagtaac tgataattac attgaaataa ctataccaaa aaactttgtc
    32461 aatcttagga aaataatggt gagttataag acgatcatag aaaacccaga acaaataaat
    32521 tttgagaatc attcggaagc ttggtttaaa gaatttaata aacctgctgt tgatggagaa
    32581 agtttcaatc atacagttaa aaatatttca gcttctggtg gagtcaacgg tacagttaga
    32641 ggcgaattga agattttcaa atatatcaat gacacagaga ttggtatccc aaatgtgact
    32701 ttcgaattaa gacgagcaga tgaacagcct attcaaggac agtctagtat cctgttgact
    32761 tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag taggagacta tgttgtaaaa
    32821 gaaaaagaag cacccaattg gattgatttt gatccactaa gttcaaatga actcaaattc
    32881 tccataaacg aaaatgatac agagggagtc agtttaccta tttataacaa gaaaaaggtt
    32941 acgaacatca cagcaactaa aatttggaac ggtggaacca ctccaagacc gagtatttat
    33001 tttaaattat tcagagcaag tacaaacaat tgggaaccag ttcctgatgc agaaacaaaa
    33061 cgattagaca atggaataac ttcagtgact tgggaagata tccaacagta tgatgatagt
    33121 ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa acggaaatga ttatgtccct
    33181 tcaggttatc aaaaaattga aaacgggcta gtcgttacaa atgagaataa agaagtcatt
    33241 tcagtctcag gacaaaaaac atgggaagac aatgagaacc aagacggcaa acgaccgacg
    33301 acgatcacag taaatctatt agccgacggt caaccgatcc aacacaaaga agtaagtgaa
    33361 aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat acagagacgg gcaagaaatc
    33421 atctatactg taacggaaga caacgtgccc gaatacagta cgacgattga cgggtataac
    33481 atcaaaaaca gttatacacc ggaaaaaaca aatatatcta taccaggtag ccatataaca
    33541 ccgtggaaaa attttccaga gaagacagag agagagaaaa ttagaaatct attctctcaa
    33601 aaattgcaaa tatttgctca tacaggagaa tccagcgaac aaagaagcaa ttatattagt
    33661 aagagagctg tacctaaaca gataacaaat aaatcaaaaa gcattcttcc taaaacaagt
    33721 agtaaaaaat ctagttttgc tgtaatcatt ggtttattga tagtaactat ctgtg
//
LOCUS       DACCKU010000022        32657 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.022, whole genome shotgun sequence.
ACCESSION   DACCKU010000022 DACCKU010000000
VERSION     DACCKU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32657
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.022"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(94..390)
                     /locus_tag="IVN13_000991"
     CDS             complement(94..390)
                     /locus_tag="IVN13_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP7011494.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            746..1102
                     /locus_tag="IVN13_000992"
     CDS             746..1102
                     /locus_tag="IVN13_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7011495.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1112..2011
                     /locus_tag="IVN13_000993"
     CDS             1112..2011
                     /locus_tag="IVN13_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7011496.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGKLLDHYSNIKQVVF
                     GHTHRRFEDQLIHGAIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            2528..3259
                     /locus_tag="IVN13_000994"
     CDS             2528..3259
                     /locus_tag="IVN13_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7011497.1"
                     /translation="MMKKEIVLTATTMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRGTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            3404..5431
                     /locus_tag="IVN13_000995"
     CDS             3404..5431
                     /locus_tag="IVN13_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP7011498.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENENLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            5539..6636
                     /locus_tag="IVN13_000996"
     CDS             5539..6636
                     /locus_tag="IVN13_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7011499.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMRQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVIIETKITKKGHSKVLYREEKEALQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            6942..8410
                     /locus_tag="IVN13_000997"
                     /pseudo
     CDS             6942..8410
                     /locus_tag="IVN13_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            complement(8503..9237)
                     /locus_tag="IVN13_000998"
     CDS             complement(8503..9237)
                     /locus_tag="IVN13_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7011500.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            9563..9973
                     /locus_tag="IVN13_000999"
     CDS             9563..9973
                     /locus_tag="IVN13_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP7011501.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAGRVSVNEMEENQID"
     gene            9966..10667
                     /locus_tag="IVN13_001000"
     CDS             9966..10667
                     /locus_tag="IVN13_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP7011502.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            10667..12280
                     /locus_tag="IVN13_001001"
     CDS             10667..12280
                     /locus_tag="IVN13_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7011503.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHTFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKKTIKTITKKEKKDDI"
     gene            12270..12491
                     /locus_tag="IVN13_001002"
     CDS             12270..12491
                     /locus_tag="IVN13_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP7011504.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDECDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            12488..13249
                     /locus_tag="IVN13_001003"
     CDS             12488..13249
                     /locus_tag="IVN13_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP7011505.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEELSLRFPEIFAYALGDVRQMKELQQAVSKCQDRFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            13754..14263
                     /locus_tag="IVN13_001004"
     CDS             13754..14263
                     /locus_tag="IVN13_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7011506.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVVVGSVSTLLVLLINYKITSR
                     IKNMKIKIAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(14334..14873)
                     /locus_tag="IVN13_001005"
     CDS             complement(14334..14873)
                     /locus_tag="IVN13_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP7011507.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVAVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(15013..15624)
                     /gene="rpsD"
                     /locus_tag="IVN13_001006"
     CDS             complement(15013..15624)
                     /gene="rpsD"
                     /locus_tag="IVN13_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP7011508.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            15940..16737
                     /locus_tag="IVN13_001007"
     CDS             15940..16737
                     /locus_tag="IVN13_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP7011509.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAYGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPAATILV
                     EGMFANIVVNTAVLISMRMKEDAGKVITVVFIIFIFAFLGYEHVIANFPAFTLAYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            16765..17400
                     /locus_tag="IVN13_001008"
     CDS             16765..17400
                     /locus_tag="IVN13_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP7011510.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLTLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            17420..18193
                     /locus_tag="IVN13_001009"
     CDS             17420..18193
                     /locus_tag="IVN13_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7011511.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHKDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            18601..19398
                     /locus_tag="IVN13_001010"
     CDS             18601..19398
                     /locus_tag="IVN13_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP7011512.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            19376..19789
                     /locus_tag="IVN13_001011"
     CDS             19376..19789
                     /locus_tag="IVN13_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011513.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEQEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            19773..22418
                     /gene="mutS"
                     /locus_tag="IVN13_001012"
     CDS             19773..22418
                     /gene="mutS"
                     /locus_tag="IVN13_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP7011514.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAADVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            22436..24544
                     /gene="mutL"
                     /locus_tag="IVN13_001013"
     CDS             22436..24544
                     /gene="mutL"
                     /locus_tag="IVN13_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP7011515.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEEPKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKFVEEDEKIYEEELALHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            24566..25126
                     /locus_tag="IVN13_001014"
     CDS             24566..25126
                     /locus_tag="IVN13_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP7011516.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(25211..27199)
                     /gene="treP"
                     /locus_tag="IVN13_001015"
     CDS             complement(25211..27199)
                     /gene="treP"
                     /locus_tag="IVN13_001015"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP7011517.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            27422..28135
                     /gene="treR"
                     /locus_tag="IVN13_001016"
     CDS             27422..28135
                     /gene="treR"
                     /locus_tag="IVN13_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP7011518.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFIEFARRRKV"
     gene            28212..28658
                     /gene="msrB"
                     /locus_tag="IVN13_001017"
     CDS             28212..28658
                     /gene="msrB"
                     /locus_tag="IVN13_001017"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP7011519.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            28828..29430
                     /gene="ruvA"
                     /locus_tag="IVN13_001018"
     CDS             28828..29430
                     /gene="ruvA"
                     /locus_tag="IVN13_001018"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP7011520.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            29443..30444
                     /gene="ruvB"
                     /locus_tag="IVN13_001019"
     CDS             29443..30444
                     /gene="ruvB"
                     /locus_tag="IVN13_001019"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP7011521.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            30473..31051
                     /locus_tag="IVN13_001020"
     CDS             30473..31051
                     /locus_tag="IVN13_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7011522.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            31103..31585
                     /locus_tag="IVN13_001021"
     CDS             31103..31585
                     /locus_tag="IVN13_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP7011523.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIGENKITNNL
                     "
     gene            31702..32076
                     /locus_tag="IVN13_001022"
     CDS             31702..32076
                     /locus_tag="IVN13_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011524.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT        10841 a         5688 c         6828 g         9300 t
ORIGIN      
        1 aaagcaaatc gtagttgtca caatctcttc taaacgatgt tccatcagct gaccttcggt
       61 attagctcaa agcactgatt tcacaacctc tatttaggca tgtgtctcag caagcggttg
      121 atagttgatg atctgcattt ttcgataaaa atcggcggga agcttactcc accgttttaa
      181 taattgatag aaataactct cttctgcttt gattttatac tcgcttaata tcaatccaaa
      241 actattcgtt ttttttgaag ctaatttttt ataaatatcc aatgctttgg ttttatcttc
      301 attgacaaag agtgttttta cttcaatata agaaccctcg atattttgtg tttcttcgat
      361 aaaatagatg ttttttatat attccttcat agtgattcct ccaaagaagt ctttttattt
      421 gataagttta ctctaccgaa gagttgttaa taatccgtga acaaaaagta attctttttc
      481 caatagttgt taaaggtttt ttatttttta tagtattttt tgttatttaa gaaaggaata
      541 aggttaaaca aataacaggt tgctttttgg gtctttttcc gttcaagaaa ctgaacgaag
      601 aaaagggaaa gttgtctctt ttgtgataag agaaatcata ataaacgaat gacttacttt
      661 ccaataaatt gagaacagtc agaaccagac aaattagttg catctgatga ataaagtgat
      721 tattctatga catgaggtga tacagatgaa attagaagtg acaaaagaag cacaagaagt
      781 attgaagaat aaattagaag cagacgatca acttttatta gatatcgaaa acggtgacgg
      841 tccatttgca gaaagccaag tcagttgcca attggacacg gcatttcgat tgatcattgt
      901 caaaaaagat acacaaacag atctaagtct ttattctgta gtggctgata caccagttgg
      961 accaatcaaa atcaaagaca gtgcaatact ttacatggat gatccatcca cacttgcttt
     1021 agaaccaact tataatagtt tacaattaaa aggaccaagc ggcttgagaa aaggcaactt
     1081 gcaagttgtt cgaaaagact agagggataa aatgggaaaa ttggcgatca tcagcgacct
     1141 acatgtggac atcaataaat tcggcgaatc agagctgatg cagctttggc aagttttgca
     1201 aaagcacaag atcactcggc ttcatctagc cggagatacg gctaataaag tagaccgttg
     1261 cctagctgtt gtcgaatttt tcgtcaataa aggattatct acgacatttc atttcggcaa
     1321 tcacgagttg gcagatgtgc ccaatgaaca gatgatggac cattttccag acccacattt
     1381 tttaaatgaa cggtatattg ctttaaatga gcagaccgtt cttttaggga tgaatggctg
     1441 gtacgactat cagttttccg aattgaaaga ttacgaggca atcgtacgaa tgaagcgttt
     1501 gtattggtat gatcggttca tccagcgtgg gaaaacagat ctcgaggtca atgcagaaat
     1561 gcttgcagct gcaaaaaatt tgctagacca gctggaagca aaaaaacttg aggtgatttt
     1621 atccactcat ttcgttccta aacgggcatt tatcatgtat caatctgctc cttatgagcg
     1681 ttggaacaaa ttaaatgcgt ttttaggatc agaatcattc ggaaaattac tagatcatta
     1741 ctcaaatatc aaacaagtag tttttggtca tacgcatcgg cggttcgaag atcagttgat
     1801 ccatggagca atttatagtt gtcgaccatt cggttattat tatgaatggc aactgactcg
     1861 tgattttgtt ttgaaagaac agttgttaga cagctatgat cctatgaaat tacgtgtact
     1921 cttgcgtgcc catcatccgg cctttcaagc ctatcaaaca caacatttgc aagaggaatt
     1981 tgaacaggcg atgacaatcg ttccgtattg agacagcatt agtaatgtgt aaaagaaaat
     2041 accttgtaaa acataaggat aagtaagagc agtagatcca tttatcgtgg atatgctgct
     2101 ttttttgtgg aaaataatca gcgaatgttt atttgatcgg atgatttgta actcgcagag
     2161 tactagatgc aagcgtttat tctttttttg aacgataatc acaaaaaaaa atataatata
     2221 cattttttat ttaattataa ttaatattga ttgaagtcta ggcagtcaag tcacattttg
     2281 atagatagga gggggactta cgaattattt taaaggctat gacagctgta tttctagatt
     2341 gtgtttgcat caagttgcaa taaaaactag aaatcaaatc gggctgataa tcatttataa
     2401 ctaatcgata cataggacca aatataggtc tatgttcttt tatttcataa gtatcgtaaa
     2461 acagaatttt gctatggatt aatccaatcg actgatatgt ttagtaaata aagaaagagg
     2521 aatgtcaatg atgaaaaaag aaatcgtatt aacagcaaca acgatgttat tcagcatggt
     2581 agcaagtacg acttttgttt ctgcaactga agtataccca aaggaatata acacagaagg
     2641 aacgattaca tttgaagcag gagatgaggg tgtaactcct cctgttgatc cagagaatcc
     2701 tgatcccaat aaacctgtcg atccatcaga tcctccaagc cctggaacag gaggggcatt
     2761 atcgattgat tatggatcaa aattcaaatt tgggactcaa aaaatttcta ctgcggataa
     2821 aacgtattat gcagctgctg atgtgatgaa cgatgggtct agaaaaccga catatgtcca
     2881 agtgacagat aggagaggta ccttatctgg gtggaaatta agtgtctctc aaccagaaca
     2941 gtttaaaact gccagtgggg atgaactagt tggagcacag ctgaaattta cgaaagggca
     3001 ggctgtttct ttggttgatc caacatatac gccgcaaacg gtgaattctg aattaacgtt
     3061 gacaccggga ggaaacaata cgttagcaat taatgctaaa tcaggaacag gtgtggggac
     3121 ttgggtctat cgatttggtg caaatgaaaa tgaaaatcaa gatgcggtac agctttcggt
     3181 acccggtaaa tctgtcaagt tagcgcaaca atattccact aaattagttt ggacattaga
     3241 agatacacct aacaactagt ttactgaaaa gaactgttaa aaataaatac gtagtcatat
     3301 tgtcttgcga ggtaatcgag caaaaattgt caaaggctag gctaaatttg tttggatacg
     3361 aatagttcat ctaggaattt aacgatcagg aggaaaagat atattgaaaa agaggaggat
     3421 cgtgctcatt ttaagcagtc tcttagtgac taatatcttt ccgatcatag ggtactcttt
     3481 ggaattgaat cagttggagc atggaaatag aagagacgaa gaaagcaaac tcacttcgac
     3541 attcgacagt ttaatagaat ccaaccagac acaagaaagc agtgaggatt taagtatttc
     3601 gagtagtgga gagaccactg acaacgtaga agctgagggt tcggacaaac aaggactgga
     3661 agatgcacca atttctgcgg ttgagctgcc tgacgatccc aatattattt ctatcgataa
     3721 aatttttcaa acacctatcg gtactagcac gtccatattg gaaaatggga agttactgca
     3781 gttgaaccca gcagaaaggt ctcaaagagg agctatttgg tcaaataaga agattagttt
     3841 actctcggat tttacattta aatcttattt atacttagga aatcaacgag atagagcggg
     3901 agatggcatg acctttacat tgactggcga tcctagaatg gctagtaatc ccagtcaagt
     3961 tattggatcg ccaggtatgg gaattggctc atattctact cgttctggcc aaccttacat
     4021 tgcgaatgcg ctctcgattg agtttgatac gtataaaaat agtggttctt ctgatcgaat
     4081 ggatcgagag ataactgctg ataatagagg gcatggacat gtcgcttttg tcacgcctaa
     4141 accaaataat aataattatt ctggagagca tactggcgtg acggtagctc ctacatttct
     4201 ttctaatggc acatggcgct tgttaataat taaatgggat gcggagcaaa gaagtcttac
     4261 ttatgatttg gaaggtgtcg gtcgctcttc tcggattata cctgatctca ttaggcagtt
     4321 tggttctaca gaagtaaatt gggggtttac ttcttcaaca ggtacttttt ttcaagaaaa
     4381 tgctgtcgct gtcacgcgac ttccatcgaa tgtcacatca acagctgatg taaaagtcaa
     4441 cgatagtgat tatggggcca tcgctgaagc tggaggaaac gataagataa ctatcagaaa
     4501 tacattgaag gtcgaggggg attttcttga atataagaaa atgcagacta gtatcgacct
     4561 cccacaagaa ttaactattc ccgagcatat cttgatcaat ggaaatcaaa ttgatgccaa
     4621 agatatcttg ataaccggaa atcgagtcac gctaaatctg acagattatt ttattgctgg
     4681 agatacgtta ataatcgaat tggagacaac tttgcaggaa gtaattcctg aaaaagtact
     4741 cactatgcat tttgaatacc ttgaaaacga gaatttgatt gagcaatcga atgaaataaa
     4801 cctaaagatc gcaaagctga aggagataga tatccatgtt tattataaag ataaagacag
     4861 tcagattgat attgctgaaa tgaaaacaat cactgggaaa ataggagaga agtataaaga
     4921 aactcctatt gatattgaag ggtatgtctt tgactcggat tcagcgaata cagaaggcat
     4981 cttctccgaa aatacagagg atatctattt ttattacaga aaaggcaaac tttatctttc
     5041 aactgctcct caatatgtca attttggaaa acataaaata tccagtagac cattaagtga
     5101 attcggacag ttttcaggtg gattgaaaat tatagatgaa cgggcttttg gaagttggaa
     5161 attacaattg aaacaagaga aagttcttac aaatggagat tttgagcttc cagaagctct
     5221 ttcctttgtt acctctgaaa atacaacggt tatagggaca tccgctgtta cgattttcga
     5281 aagtgatcaa aagggtgaaa gcgatttgtc ctctctttta gattctacag ctcatcgtgg
     5341 gatcagaata gatattcctg tagaaaacca aagaatcgga acctttgagg ggaaactatc
     5401 ttggatactt gcagatgtcc caggaaatta acaggaagag aagaatataa caaaattatt
     5461 gagtttcaag aagggcacag gtaccatagg ctcttgtgtc ccacagagac aagatattgt
     5521 tatagtcttc tcttacatat gattaaagga gcgattttct tgagaccaaa acaacaatta
     5581 tttataacac tcctagttat tttaggggca ctttttaaca atatatcagt gtcagctagt
     5641 gcatttaatt tttcagtgac gccgataact tcagagaatc aaatcgacaa gagaaaaact
     5701 tacttcgatc ttcaacttga tccggatcag gaagtagaag taaaagcgga attgagaaat
     5761 gatacagaaa aagaagtgaa aatcgatatt tctgtgaata gcgcaaccac caattcaaat
     5821 gtgatggttg aatacggaaa aaacgagatt gaaaaagaca tgagcctgat ctttgatctg
     5881 gtagattatg tgagctatcc acagatagtg acgctaaaac ctaaaagtgt ccaaaccgtt
     5941 gcttttcatg tcaggatgcc gaatgagcga tttgatggtg tattggctgg tgggatcaca
     6001 ttccaagaaa tccagacgga aaaggaccaa acagaaacta aggatcaaag cttatcgatt
     6061 gagaatgaat atacctatat tattgctttg ttgatgcgac agacactgaa cgaagttgct
     6121 ccgaacctta ctcttcatga agttaaacca gatcaaatca atgcaagaaa tgtcatctta
     6181 gcaaacgttc aaaatgatca aaaaacttac atcaatcaag taattattga aacaaaaatt
     6241 acaaaaaaag ggcattctaa agttttatat cgagaagaaa aagaagcgtt gcagattgct
     6301 ccgaatacaa atttttcttt tccaactgct ttaaatggtc aaccgctaac cccaggtgaa
     6361 tatcacctta caatgactgt tttaggaaat gaaaatgaaa atggaaaatt ttcgagaaaa
     6421 aaaggaaata ctacgatcaa ctataccaat caatgggtat ttgaaaaaga ttttacaata
     6481 gacggaaaag tagccaagga attgaacacg acggatgtta cgattaaaaa agacaataat
     6541 tggatttata tcttagtagc ttcacttttc ttattgttta ttttattatg catacttttg
     6601 tttgttttga gaaaaaaaga agagaaagaa aagtaaggtt aagataacaa atagtaaatt
     6661 tttattattg taggatggtt gggctctttt tataataata taaaatatac cattttatta
     6721 ataaaaatgc atactagtaa atgatataat tcttataaca ggtttgatcg cgagaaaggc
     6781 caaataaaac gctctccaaa atgttaaaga aagacaggac gatgtatatt atacaaacaa
     6841 gaatcgctga caacagtgac tcggatccag gaaatcagtt ttatccccag ctagttggtg
     6901 aatgcatgga aagtaatata cgtagaaaga gctgatgact aatgaattat gagttttttt
     6961 taagcaaaga agatcacaga agttattcat tacttaaata tttagaagca agctcaaatt
     7021 tatcagagtc aatatcgaat gttcaagaag agttgtcgct atcgacattt ctcttgaaaa
     7081 aaacgatcga taagctgaaa catgatttac aaaagttcga tctagagaaa aatttgcgag
     7141 taaccgtcag tgaattggat atatgtctgg aaattaatgg aaaatgttct agcaaagctc
     7201 tgttatctcg ttatataacg gattctttat ctatgaaaat ggtattggct ttctttcagg
     7261 aaaaatatat ctcagtagaa aattttgcta aacagcatca cgtgagttat tctgtggctt
     7321 ataaagtatt acaaagacta aaaagaaatc taaaaaaata tcaaattttt ttgaaaagag
     7381 aaagttgaca gggaatccta aaaacattcg gcttttttta tataatcttt ttaactcaac
     7441 agagataagt tttgtggaat tgtatacgct acctataatt ataaaaacag aaaaaatgtt
     7501 aaaagattta aaacagtatt atcctctgtc tgcttatgaa aaaaagaaat tattccactt
     7561 tttagcaatc aacctgttgg ctgaacaagc tgattcttta gatattgcta gcaatcagtc
     7621 gctatttttt tcggatatag gattttacca cttaaaagga gtattaggag aaaaatccgt
     7681 tatttttatc aataagacac tctcttggct ttatcttcat gataaattgg atcaaaaata
     7741 cttgagaaaa aatgaagatg aaacaatcga atggttgaat gaacgattca ttgaaagttt
     7801 tgaaaatcag tttaatctgt tgcgagaaga gacgagccaa ttgatccgaa gagagttaac
     7861 caaaatacat tttaaacttt tgcattacac tattaataaa gctgatgaat ttccacttga
     7921 tgtttctttt tttagagaaa actatccagc attttattgc tatttgatag agtatataaa
     7981 tgatctaacg tcaaaacgca aagagttgct tcaagataaa ttttttcttt ttttcagtta
     8041 tttattatta ttgatcaatc acatacccat tcaactgatt tctgaaccag taaagatcat
     8101 catcgatttt tcttatggtg ctgcctataa tcaatttatc aagaaaaatt taagcttgta
     8161 cattaatctg aacacggaag tgatagaggc aggaaagcct gagttcccgg atgttataat
     8221 cacgaatagg aataatctat atgaagaaag tgcatctcaa gtagtggttt ggctcgatcc
     8281 gccaagagca gtagactggg ggaatcttac aaagattttg ttgactatcc aagaagagaa
     8341 atacaaagac agcaaaattg acaagcaaat agacgatttt cctgaagaaa tcatcgacag
     8401 gatcgaatag tgaacttctt ttagaaagtc cgactgcttt ttctaaaaat aaaaaaactt
     8461 ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca taaataataa
     8521 tttatataag aatactgcta cgacaccagc acagataggt gctacaactg gtacccatgc
     8581 atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta caagacgtgg
     8641 agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg aagcaactaa
     8701 tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt ggaagaatgg
     8761 tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga attcattgac
     8821 gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta acacatggtt
     8881 cacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca attgtccaat
     8941 cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag ggaatagacc
     9001 attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga ttccaccaaa
     9061 aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca tccagtcact
     9121 gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac ctagaacgat
     9181 tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt aatccataaa
     9241 tacctccaaa agttataaaa atgcaaaaaa aaagaaacat caacacgaaa agcatcggtg
     9301 cagtgtttcg tgtcggaagg atttctgcag tcttaacaaa tgtgaataaa aaaatgaatt
     9361 ccattactta taagaaacca gaccaacagt aaaactccaa ctctttttaa aagttctcca
     9421 caaatcacca agcttaacta taaatatacc aaaaaaaaaa cgtttttttt ctcaaaaatt
     9481 gtatcttaga aacagcatgt tttgaaactt tcagaaaagg tttgttagta aaatgaaagc
     9541 tatacgtaag gagtgttgtt tgatgaaatt ttttcaagag tttttcatta ttattttctt
     9601 ttctgtatta ggagaaggca tacggatttt tgctcattta cctattcctg gaagtatctt
     9661 aggtattctt tttttatttt tagcatttga aatgcatctt gttgatcctg aaaaaatcgg
     9721 agcaactggt gattttttat tgaataatct agctatttta tttgttcctg ctggtgtagg
     9781 tttattggaa tattttgatg atattgcttc gatatggcct gttttactgg gagcagtcgt
     9841 cgtgtgttcc atcgtcacga tggttgcagc gggaaaaacg gcagaaggtg tagaagcttt
     9901 gctgggctat gtccgtaaga aaaaagcagg aagggtatct gtaaacgaaa tggaggaaaa
     9961 tcaaattgac tgagtttttt gtgaatccac tatttggctt aagtttgact ttaggagtgt
    10021 atgtattatc tgataaattt ttgcggaaat ataaactgcc tgttttgaat cctctcgtat
    10081 tatcgattgt tgttattatc ttatttttgc atttttctgg gatcagctat aaagactata
    10141 atatcggggg caattttttg actatgctga ttactcctgc gacagtggca cttgccatcc
    10201 ctctttataa aaacttccgt ttactgaaag agaattttta tcctgtaatg gctgcgattt
    10261 tagcaggaat agtcgcaaat tgcttggtta gtgtagggtt cggttacttc ttctctcttc
    10321 ataaagagat ggtgatctca ttactaccta aatctgtaac gacggctatt tctgtcgatt
    10381 tatctcatac gatgggcggg gtgaatgccg tgacacttgc tattgttgtc tctacaggta
    10441 tttttggttc gctgatcgcc actcatattt tccgcctatt caagatcgaa agtccagttg
    10501 ctcgcggtgt ggcgttagga agtacttctc atgctattgg tacagcaaaa gcaatcgaga
    10561 ttggcgaaat agaaggaatc atttccggac ttgcgatatg cgtcaacgga atattgaccg
    10621 ttttattgct accgttattc tttcagcctt ttgctggctt gttttgatgc gaaaaccact
    10681 attttatgct acaataacca aaacgttcga gcataaaaaa ggagggaaag acgtgtattt
    10741 gtcttccgag tatcttaaac aatttgataa agatctatat tataagattc tcctcctaga
    10801 aagttttgag gatcaagctt ggcataccgc tgcacaatta gcgcaagttg ttcagctgga
    10861 tgcccgcagc gtgtcgaaat atctgaacga gctgtctaaa aactatcagc agtttagcgg
    10921 taagacacac cctttgttta caaaaaatca ccgttctggt tataactttt atgatacgct
    10981 cgattcaatt gaacatgagc gttttttgat ctatctcgtg caaagtacgc tgaaattcca
    11041 gttgctgcac gacatttttt ttgaagaatt ccatacaatg taccaatttg cgcaaaagca
    11101 ttacattagt gaatctactg ctcatcgaaa aatcaatgaa tggaaacagc aactgcaaac
    11161 ttacggaatt cggctgcaac gaggtacata tatcgctcaa ggcgaagaag aaatcattcg
    11221 actttacttg catatgacat tctggcagtt gtttcgaggg aaaatctggc catttgaaac
    11281 gatttctcaa atggacgtaa agaatatggc agaacatatc atggcttttt ttaatgttcg
    11341 tctgaacgag atcaaaaaaa gacgattgga atacatgcta ggtgcctttt ttttaagaaa
    11401 atcacagaag cattatgttg tattgaatga gaaaaaaaga cggctgattt ctgacaatct
    11461 gttgttccag cgattttgcc aagtcatgga gccggtattt cctaattatt ttcaagtaga
    11521 agacgagtta ggcgcattat ttcttgtact gatgactcgt gaagaatatt atagtgatcc
    11581 taagatacgg aagaaaattt ttgattttca tcaacaagca aaaacaccgc cctttacagc
    11641 cctttcagaa gcaaaagccg ctctttctct ttatcaggaa gagcaggggc tgccagcaga
    11701 aaatctaacc ttcgaggcag aaaactattt attcagcagt cattttttcg cctatctttt
    11761 tcctaatgcg aaagaaacga ttgatggcaa tagtagtgat tttattaatc atttagttat
    11821 tgaaaacaag gaattgaaac aatggctcgt acattttttt gaaagtagac acaaacatcc
    11881 caatcatctg gcatttaaaa atcatacgtt tttgatgggt cggtatctga ctgtgtttaa
    11941 aacattagga gcctttactc cacagcttcc aaagatcacg attcttttaa tgacagattt
    12001 cccattattt gaagagcaac tgttagagga gggattacgg acatttttcc gtaatgagta
    12061 tcagctgatt tttctgccaa cagattatcg ggggagagaa gttgatcttc tgatttcgac
    12121 aagtaaagtc caccgcaaac catgggcgga tcttgactat tttattgtga ccgaagaact
    12181 aaaattaatt gattacatcc aactatctca aaaatttgaa atgattcaaa aaaaaactat
    12241 taaaacaata acaaagaagg agaaaaaaga tgacatttga agaaatttta ccagaattga
    12301 aaaaaggtgc aaagattatt cgtaaaggat ggagtggatt tgaactttat gttgttctcg
    12361 tatccgatga tgaatgtgat ggctgtccag tgacacctta cttattgatc aaaacaagtg
    12421 acgaaggttt ttccagcttc gctcctacag tttgtgatat tttagcagat gattgggcga
    12481 tcgtagaatg aaaaaccagt atccagatct agctaaacag gttgtattta tcacaggagc
    12541 tgcttctgga attggtgctg cgcaagcccg agcgtttctt gaacaagatg cttttgtatt
    12601 tggattagat caaaagttcg gtaaaatgga agaactctct ttgcgatttc ctgaaatttt
    12661 tgcctacgca ctaggtgatg ttcgacaaat gaaagaactc caacaagcgg tatcgaaatg
    12721 ccaggatcgt tttggtgaag ttacgatttt actgaataca gcggggatat tggatgccta
    12781 caaaccattg atggaaactg acgaatcgct gtgggatctg atttatgaga cgaatgtgaa
    12841 aagcatgtat cagctgacaa aaatcattct tcctgatatg atccgccaaa aaagcggaac
    12901 gatcatcaat atggcttcga ttgctggctt gattgctggt ggaggtggga tcgcttatac
    12961 aagcgcgaaa catgcgattg tcggatttac caaacaactc gctctggatt acgcaggaca
    13021 gggtatccga gtaaaaggaa ttgctccagg agccattcaa acaccaatga atgcggctga
    13081 ttttgaaggg aaaggtgaga tggcagagtg ggtggcaaat gaaacaccag tcaagcgatg
    13141 ggcacagccg gaagaagtag ctgagctgac gttatttctt gcctctcctc aagcaagcta
    13201 catacaagga acgatcatcc caatcgacgg cggatggttg ttaaaataac cttgtgtttt
    13261 tttcaagctt ctgtatgata aaactaaaga gagaaacaac ggttgatgat aaaaaaagtc
    13321 gatgactgtt cccgacattc gatgccgaac agccagttca aaaaattctt tttaataatc
    13381 gttaatattc gaaactaagc ggcttttcac gaccttttga tataaacggt gttcaaaagc
    13441 ttgcaactgc ggtaacaagt ccagccaaat gaaaaatata aggagctatt tttatttgac
    13501 tattcttatt acttgttgca gggcagcttt ttcactacct cttgatataa acggtgttca
    13561 aaagcttgca actgcggtaa caagtccagt caaatgaaaa atataaggag ctatttttat
    13621 ttgactattc ttattacttg ttgcagggca gctttttcac taccttctgc ggcacttcag
    13681 ttggaatttt cttcccatcc tttggtcgcg gtcattacca agataaaatt atcttgcaaa
    13741 ggagaaatag aacatggaaa aaaataatac ccgttcagcc aaacgctgga caacgacaga
    13801 tgtgactcgg atggcgctag tcactggttt atatgtggca gtgacgctgg ttttgtctgt
    13861 ctttagcttt ggagtgattc agattcgtct ctcggagatg ttcaactatc tgtccttgta
    13921 taacaaaaga tacatcgtcg cggtaacagc gggagtagct ttggctaata ttgcttctcc
    13981 tcttggactg atagatgttg tagtaggaag cgtcagcacg ctgcttgtat tgcttatcaa
    14041 ctataaaatc actagtcgca tcaaaaacat gaaaatcaaa atagctgtga cagcatgcgt
    14101 atttgctttt tccatgttta cagtagctgg tcagctaacg attttatatc aactaccttt
    14161 tttcttgaat tggtgggtga ttgctttagg agaattggca tcaatgattg tcggtgggat
    14221 aattatttat tggatcggta aaaaagtgga tttgtcaaaa taaagatctg tttaatttaa
    14281 aatatggcag catggaagaa gactttgagt cttcttccat gctgcttttt tgattaattt
    14341 gtatgaagaa atcgatttag ccgttttttc atcaagatac cgatataagc tgctccagtt
    14401 gccttgattg cttccggcag taaaaacggc agtagtccac tggcaaatgc agttgtaaaa
    14461 gtaagatcac cacttatttt cagccagatc gttccgcaga tcaatgcagc aagagagccc
    14521 attagattgg caaagattgc atacaagtaa tgagcatccg ttctttcgat catccaacca
    14581 gttatccacg tttgtaagag aaatccaatc aaatatccgc cagttggccc aaataaaaca
    14641 gctatacctc cagtcatccc tgaaaataca ggaagaccaa tcaagccaag taaaagataa
    14701 actgcaacag atatcaatcc tagttttctt ccgagaaaag ttgctgttaa tccaatagca
    14761 aatgtttgcc ctgtcaaagg aaccaaacca aaaggaatgg tcatctgaga aaggacagaa
    14821 ataatcgcgg caaattccgc agcaagtatc aattctttca atgttcggtt cataacgccc
    14881 tcttttcgtt aactaaatat ttttatttgg ttaacgaaat tgtaaaacaa agtgattact
    14941 ttgtaaatag aaaatataaa aaaagaccag ctatccaata gatagcggtc tttttcaaga
    15001 aagcttttga aattataagc tttggttgta gtattcaacg acaagagctt cgtcgatttc
    15061 tggatatagt tcgtcacgtt ctggtaaacg agtcaatgaa ccttccaatt tttcagcgtc
    15121 aaagcttacg aatgctgggc gtccaacagt tgcttctaca gcttctttta ctgttacgat
    15181 gttttgtgat ttttcacgaa ctgagatcac ttgacccact tctacgtggt atgaagggat
    15241 atcaacgcgt ttgccatcaa ctaaaatgtg accatggtta actaattgac gtgcttgacg
    15301 acgagtcgtt gcaagtccta aacggtaaac aacattgtct aaacgttgtt ctagtaagat
    15361 catgaagtta acaccatgtt taccttcttt gattttgcta gcttttacga acaagttacg
    15421 gaattgacgt tcgttcattc cgtacatgtg acgaagtttt tgtttttcag ttaattgcat
    15481 gccgtattct gattgtttgc cacggctgtt aggtccatgt tgacctggtg cgtaagggcg
    15541 acgtgctaat tcttttcctg tgcctgacaa tgagatgcct aagcgacgag aaattttcca
    15601 tgatggtcct gtataacgag acattaaaat ttcctccaat gaatataagt ttttggagta
    15661 aaataatccg ttgaaaaatt cataatcata tagtttgttc ttcaatcttc acctttgcag
    15721 ccgcggttac acaatcgaac ccgagacggg gcaacaaact agggatgagt tattattttt
    15781 ctgctgcatt attttacaca aactacagta tatgatagat catccatgaa tgtcaagata
    15841 attagtacat aaaaacgctt tacaaaccta ggctgtcttt ataaaatgag agggtatgtg
    15901 caaatggaaa aaagcgaagg aagtttaagg agaaaatcaa tgaatccggt atcaccatta
    15961 tttgagaaaa tcgatgcttc aattgaaaag aaggcgaatc tttttgaaaa cagcttttca
    16021 cgttacgctg ttcgagcggt tctcgcctgt ttgtttttga ctttaggaac ggctgtggca
    16081 tttgcgatcg cgatgaaggg ggaagatatc gcgtatggtc taggaaagat tttatacgca
    16141 tttatgttca gctggggtct ggtcatgatt ctatacatga acgccgaatt aggaacatcg
    16201 aatatgcttt atatgaccgt gggtgttttt cggcagagac ttgctttaag taaggcagcc
    16261 aagattctat ttgcctgtat ttttttcaat atgattggcg gtatattatg tggatttcta
    16321 atttctatga ctacgccatt tcaagattta acgttggaca actttatgtt agaatcaata
    16381 gcagtaaaac tagcaaaacc tgcagcaacg attttggtgg aagggatgtt tgcgaatatc
    16441 gttgtcaata ccgctgtact gatcagcatg cggatgaaag aggatgccgg aaaagtgatc
    16501 acagtcgtat tcatcatttt catttttgct tttttagggt atgaacacgt gatagcgaat
    16561 tttcctgcct ttacgcttgc ttactttgct tctcacggac agatggatgg aatgacagtc
    16621 agcaatgtgt tacataattt gttttttgct ttggcaggaa attatattgg tggcggttta
    16681 gtaatgggac tgggatatgc ttggctgaat cagtcaaaat catcctatgt ggactaaaaa
    16741 gtcgttcatt aaaggggaag tgccatggaa gttttagttt ttccaaaaaa tgaggaaaaa
    16801 gcgttacaaa tggaaaagta catgaaaaat cttttttcct ttgctggagt acctaagccg
    16861 gaacgcgctc ttttacaaca accgttactg gatgagagca aaaatttatc tatttctgaa
    16921 gttttagctt ttgttcatta ttattatgaa caaatcgaaa gagagtatca gtatgtagcg
    16981 atcgatttag tccagaaaaa catcaaacgg cttacatttg aagacttagt cgtattgttg
    17041 acactagttc agaaaaaaga atggtgggac agcatcgact cttggagaaa agtatacagc
    17101 acatggtgta aagcctaccc agagcagcgt gaagaagtct ttcaattgtt tgaaaaaaag
    17161 gaagattttt ggctacgtcg aatagcaatc acgttgcaat taggttataa acagcaaaca
    17221 gacacaggac tattgatccg agcaatagac gaagatcgtc aaacaaagga attctttatc
    17281 caaaaagcaa ttggttgggc attacgcgat tatagtaaga ccgatgccga ttgggtaaaa
    17341 agacagctga caacagattt gagtaagtta gctgttcgag aggcaagtaa gtatttataa
    17401 gaaagcgaga cgacaagaga tgagacgcaa attatttgct tttgatattg atggaacgtt
    17461 gttagattca aacaaacaag ctttagatag cacgagagaa gcattggaaa aattacgcaa
    17521 agccggtcat ctggtcacga ttgctacagg gcggagccga tttcatgcgc aagagatcat
    17581 tcgcgatctt gattttacga actacatttt atgtaatgga gcggccggtt ttttagacca
    17641 tgaacaagta tataagaatt tactagatcg cgaccagttg gatgcgttcg ttcaagaagc
    17701 gcatcatgag cagatcgata cagcttttgt cagcttagac aatatcaagc gttcttcttc
    17761 caatgatgta acggtgatgg aagaagcgat gcattctttc ggtgctgtct tgcctgaatt
    17821 agatcagttt tttgcagagg aacaagaaat ataccaagcg ttagcatttt tcgatcaaac
    17881 atatgatggg aagttctctt catatgataa aattcgattt gtccgctggc atgagaacag
    17941 tgtagatgtc gtacctaaaa atggttctaa agcagcgaca attctgcact tagcagaaca
    18001 agtaggcatc cctcataaag acatcatcag ctttggcgat ggacaaaacg accgtgaaat
    18061 gttgcgcatg tcaggaattg ggatagctat ggggaatgct gtgccagaag tacaagcaga
    18121 agctaagatg gtcactgata caaacgatca ggatggaatt tggaaggcat tgaaagagtt
    18181 aagcgtgatt taaaagaggt tgtgaaaaag ctgttctgca tcaagtaata agcccagcca
    18241 aacaaaaata gctcctccta tttttcgttt ttctgggctt attaccgcag atgcaagctt
    18301 ttgaacaccg tttaaggaag aggtcgtgac agaagtggtc agtttcaaga aataagaagg
    18361 gatttacgaa aattgcttct caaatttttg taaaattacg gcttatttct gaagaagtag
    18421 atgaaacaac gtttattcgg gaaatgggac tgtgacaaga tgtttgtcat agtcccattt
    18481 ttttatttta tttttcaaaa ctaagaaata ccaggaaaga gttccttttg tttgaccttt
    18541 tctttcttat tgaaaacaac tataatgtag agtgatgtta aaagagaaga ggaatttagt
    18601 gtgcgggtat tgtttattgg tgatgtagta ggttcaatgg gacgagagat gattactgaa
    18661 tatcttccta gattgaagaa gaagtaccgt ccgcaagtaa cgattgtaaa tggtgagaat
    18721 gccgcttctg gacgtggaat cacagaaaaa atctataaaa aatttttgca agacggcgta
    18781 gatgtcgtga cgatggggaa tcatacgtgg gacaaccgcg gaatctttga gtttgtaaat
    18841 gaagctaaaa agatggttcg tccggcaaat tttccggaag ggacgcctgg acaagggatg
    18901 gtctttgtca aagtgaacca agtcgaactg gcagtgatca acatgcaggc tcgttctttt
    18961 atggtggact tggatgatcc ttttcgtaaa atgaatgagc tggtagaaga agccagaaaa
    19021 cgtacgccga tcatttttgt agattttcat ggagaaacaa caagtgaaaa acaagcaatg
    19081 ggctggtttt tagatgggaa agtttcagca gtggtcggta cacatacaca cgttcagaca
    19141 aatgatgcgc gaattttgcc aagaggaaca gcgtatctga cagacgttgg gatgacaggt
    19201 ccctatgatg gtattttggg tatgcgcaga gaacctgtaa tcgaaaagtt tctgaccgca
    19261 ttaccaaaac gttttgaagt tgtggaacaa ggccgatcga ttttaagcgg ctgtatcttg
    19321 gaattggatg atacgacagg tcatgcaaaa gaaatccagc tgattcagat caacgatgat
    19381 cgaccgttca tggagtagaa agagaggcag ttttttgcag caggagccgg aaattcaaaa
    19441 agaagtaagg aaactgacaa aacttttaag agagaatgaa acgatcattc gctacaaaga
    19501 acttgaagaa aagatccagc aaaaccagta tttagctgag ttgagagaaa aaatcaaaca
    19561 agcgcaaaaa gatgctgttc attttgccca ctatgacaaa ccagcagcag aacaagaagc
    19621 aatcaaacaa gcggatcaat tcatgcaaga atttgatcag catccgcttg ttgtagctta
    19681 tagaaaacag ctgttggaag cagatgatct gcttcatcat ttaacaacta tgatacaaga
    19741 agaaatcaat ggacaaatag aggaggaaaa acatgcctca aaaaactaaa aatactccta
    19801 tgatggaaca atatctaagc atcaaggccc aataccaaga tgcttttttg ttctatcgtt
    19861 taggagactt ttatgaacta ttttacgaag atgcgatcaa agcgtcacag cttttggaat
    19921 taacgttgac gagcagaaat cgaaatgcag aagaacctat tcctatgtgc ggtgtacctc
    19981 accatgcagc acaaggatat atcgatgcct tgattgaaaa aggctataaa gtagcgattt
    20041 gcgagcaggt agaagatcca aagacgacta aagggatggt caagcgcgaa gtgatccagc
    20101 tagtcactcc tggtacagtc atggacagca aaggattgtc ggcaaaagac aacaattttt
    20161 taacagctgt agttagtcaa ggaaatgaat ttggctttgc ttatgccgat ttaagtacgg
    20221 gagaattgaa gacggctcgg ttgcatgatg aagaagctgt tttaaatgaa gcctctgcat
    20281 tgcagacaaa agaactcgta ttaggcagtg agatcactga tacccttcgt gaacagctaa
    20341 gcgggcgttt agggctagtc ttttctcaac aaaatgagat ggaagaaaac gcggaattta
    20401 gctttttgac aagcgaacta gtcgatccgc tggaaaaaga agcaaccggc aaactattga
    20461 cttatttatc ggtgacacaa aagcggagtt tagctcatat ccaaaaagca gtagaatacc
    20521 aaccagatca ttttttgaaa atggattatt attcaaaatt caacctagaa ttaagccaat
    20581 ctattcggac tgggaaaaag cacggcactc ttctatggtt gttagatgaa acaaaaacag
    20641 caatgggtgg ccgtttacta aaacaatggc tagaccgtcc gcttatccag gcaaaacaaa
    20701 ttaaagcaag acaagaaatg gtcgcttctt tgctagacag ctattttgaa cgtatcgatt
    20761 tgcagtcagc attaacgaaa gtctatgatc tggaacgcct agcaggtcga gtagctttcg
    20821 gtaatgtcaa tgggcgagac ttgatccagc tgaaaacttc tttagaacaa gtgccaatga
    20881 tcagagggtt gattgaagga atcgatcaag gtcaatggca aagtctgctt tcagaaatgc
    20941 agccgatgga ccatctcgtc caattgatcg accaagctat ccaagatgat ccgccgttac
    21001 aaataacgga aggaaacatc atcaaagatg gattcaatga gcagctcgat atgtaccgtt
    21061 ctgctatgag aaacggaaag aaatggttgg cagaacttga agcaaaagag cgtcaagaaa
    21121 caggaatcaa gaacttaaaa atcggattta accgcgtttt cggttattat atagaaatta
    21181 caaaagcaaa tcttggaaat gcagaactgg agaagtatga acgaaaacaa actttagcaa
    21241 atgcagagcg tttcatcaca ccagaactaa aagaattgga gacacaaatc ctcgaagcag
    21301 aagaaaaatc tgttgattta gaatatcagt tgtttttagc tgtgagagaa gaagtcaaaa
    21361 aggctatcca gccgctacaa gtacttgcaa aagcaatcag tgcagcagat gtgctacaaa
    21421 gttttgcaac gatcagcgaa cgctatcagt atgttcggcc agaacttgtc agtgacaagc
    21481 atcagcttct gatcaaagac ggacgacatc ctgtagtaga aaaagtatta ggacatcagg
    21541 aatatatccc taacagcgta gaaatggcag aagacgaaat gatcttgctg attacaggac
    21601 cgaatatgtc tggtaaaagt acgtatatgc gacagttggc attaactgtt ctgatggcgc
    21661 aaatgggctg tttcgttccg gcaaaacaag cagttttacc tatttttgat cggattttta
    21721 cccgaatcgg tgcaagtgac gacttaattg ctggacaaag tacttttatg gtagagatga
    21781 tggaagccaa tcaagccctg cgttatgcta cgccaaacag tttgattttg tttgacgaac
    21841 tagggcgagg aacagcaacc tatgacggaa tggcgttggc ccaagcaatc atcgagtata
    21901 tccatcgtca tgtacaagca aaaacactct tttctaccca ttatcatgaa ctgacggtac
    21961 ttgagaaaga acttccgcag cttaaaaatg tccatgttgg agccgttgaa aaagacggag
    22021 aagttgtctt cttgcataaa atgatggatg gacctgcgga taaaagctac ggcatacatg
    22081 tggcaaagat cgcaggattg ccttccaatc tactagaacg agcagcagat atcctgcata
    22141 cgctagaaaa tggcgataac ggacaccata ctgttccagc tgaagtagaa gagaaaacta
    22201 aaacagccaa acaggagcaa ataaaaccac ctgcagaaaa agtaaatcaa gaccatatac
    22261 aagaccgtgt aaaagaagaa aaccaacagc tttctttatt cagtgaatta tccgaaaatg
    22321 aaacagaagt cattgaatca ctgaagcaaa taaatttact agaaatgacg ccaatggatg
    22381 cactaaacac attatatgaa ttacaaaaac aactgtagga agaaggcgga tatcgatggc
    22441 caaaatccaa gaattatctg aacgtttagc taatcagatc gctgcgggag aagtagtgga
    22501 gcgaccagct tccgttgtga aagaattagt ggaaaatgca attgacgccg gcagtactca
    22561 gattgatatc ttagttgaag aagcgggact gcggacgatc caagtgattg ataacggaga
    22621 aggcattttg gcagacgacg tagagaatgc cttcaagagg catgcgacaa gtaagatcca
    22681 taatcgcgac gatcttttcc gcatacgaac gcttggattt cgcggtgaag ctttgccaag
    22741 tattgcctcg gtttctgaaa tgactctgga aacggcaaat caggaagaaa aacaaggcac
    22801 ctatctgtcg ctgaaaggcg gagaagttat tgagcatcga ccagcaccac tgcgtcaagg
    22861 aacgaagatt accgtcagca atctgttttt caatacgcca gcccgcttga aatacgtgaa
    22921 aacactgcac acagaattag cgaacatcgg ggacatcgtc aatcgactgg cattgagtca
    22981 tccatcgatt gcctttcgtt tagtacatga tggaaacaaa atgctggcaa ctgctggaaa
    23041 tggcgatctg aaacaaacaa ttgcgggaat ctacggatta tctaccgcaa aaaaaatgct
    23101 gaaaatcgaa ggaaaagatc tagattttga gttatttggc tatgtttcat tgcctgaggt
    23161 gactcgtgcc agtaggaatt acttatcgac gatcatcaat ggacgtttta tcaagaactt
    23221 ttccttgaat aaagcaatcg ttgcaggtta tgggtcaaaa ttaatggtcg gtcgtttccc
    23281 tattgctgta ttggaaatca aaatggaccc gcttttagta gatgtaaatg ttcatccgac
    23341 gaagcaggaa gtccggctgt cgaaggaaaa agaactaaca gagctaatta gtaacgccat
    23401 tcaaaaagca ttgtctcaag aaaatctgat tccaagtgca gcggataatc tacggtttaa
    23461 gaaaaaagta cccgatcagc ctaaaacaga acaaatcgaa atcgatttgt cctacgcttt
    23521 tgaagaagaa aaacctaaaa agccaagtag tttgaatttt gatcgagaga caggaacctt
    23581 tttcgtagaa gaaccaaaga acacatttgt tgacagaatt gttgataagt ctgtggaaaa
    23641 tgttgaaaac tattctggtt tttctgaaga aaacaccact tatccagaaa aaacagcgaa
    23701 aacggtggat aagtttggtg aaaatgttga taactttgta gaaaaaacag tggaaaaatc
    23761 tgaaaagaaa gaatttccca ttgtggataa catggagaaa acaaatgaag aacaagcaga
    23821 taaattcgta gaagaggatg aaaagattta cgaggaagaa ttggcgctcc atcctgaatt
    23881 tgatttcact caaggaaacg acaaaacatt agaacaagcc atcaagaaat tagaaacgga
    23941 aaaaccagta caacgttttc ctcagttgga atattttgga cagatgcacg gaacgtatct
    24001 ttttgcccaa agtagcgatg ggttgtacat cattgatcag catgctgccc aagagcgcat
    24061 taagtatgaa tatttccggg aaaaaattgg ggaagtatct gatgatctgc aagaattgct
    24121 tgttccattt gtgttagact atccaaacag tgatgctttg aaattaaaag agcaaaaaca
    24181 cttgctagaa gatgcaggga tttatttaga agaatttggt caaaacagct ttattgtacg
    24241 ggcccatcct acttggtatc ccaatggaga agaagaatcg attattcggg agatgatcga
    24301 tatgctgctg acaactggaa gtgtgagtgt aaaaaaattc cgtgaagcaa cagcaatcat
    24361 gatgagttgc aaacgatcga ttaaagccaa ccattatttg aatgaagcac aagcacgtgt
    24421 gctgctgaag gatctggcac gatgcgaaaa tcctttcaat tgtccccatg gccgtccagt
    24481 attgattcac tttaccaatt cggatatgga aaggatgttc aaacgaatcc aagacccgca
    24541 ttaagtcaaa gagaagaggg aaaacatgaa gattgtatta gcatcccaat caccaagaag
    24601 aaaagagtta ttggctcata tcgtttcaga attcgagatt gaacctgctg atatcgatga
    24661 aacgccattt ccagacgaac agcccgtcga ttatgttcgt agaatggctg aagaaaaagc
    24721 acgaactgtc tgggaacaag atgaaaaaga agaccattta gtgattgcca gtgacacgac
    24781 tgttgttctc aatcaagaga tcatgggtaa accagaagat ctggctgagg ctgaattcat
    24841 gctgaaaaaa ctaagcgggg aaacacatac tgtatataca gctgttgttt taaaaacaaa
    24901 ggaacgagaa gaacggatac tagcagaagc acatgtcact ttttatcctt tgaccgacga
    24961 agagatcaaa cgctacttgg agacggggga ttatgcggat aaggcagggg catacggcat
    25021 ccaaaatgct gcgagtgtat tcgtcaaaga aatttctgga gattattaca gtatcgtagg
    25081 atttccgatt ggtgctgtca atcaggcact gaaaacattc atttaaacaa aaaagcggac
    25141 tatctgattt tcgattgaaa atccatgata gcccgttttt tttattgaaa agattactta
    25201 tctactctga ctaagatttt acttcagctt taccgactgc ttcgcccggg cgaacaagtc
    25261 ctgattggtc aagagaaagt cctgcaactt tttcattatt ggtcaaagca acaataatgt
    25321 cagttttctt gccagcttgt tctatttctg gcaaatccat ttcagcaatc agggtctctg
    25381 gtgtgacaag gtatccttct ttcacgaata cattgaaagg aacacccttt agttcaacag
    25441 tatctaatcc catgtgaaca agtacttcca gcccctcatt agaaagaata ccaatggcat
    25501 gttttgtttc aaagacactc gtcactttac cgtttactgg tgcatagact ttgccgtttg
    25561 acggttcgac agcataaccg tcacccatca ttttttgtga gaaaactggg tcatttactt
    25621 cagttatttc cttgatggtc cctgcagcga ctgcgaataa tgtttctttt gttgcaactg
    25681 ccgtaccagt tgctgaaaca ggttcgaaag actgtggcgt tgctgttgcg ccatttgctg
    25741 tttgaagttg tacatcagca gcattttcag gaacagcagg atcaatctta ttcaaaattc
    25801 cctgacgtct aaagatgatc gtcaagccaa atggaatgat gacagcaatg atcattgcga
    25861 tgatgaaagg tacccatgtt tctgcttgga tagctagtat tcctggcaaa ccgcctactc
    25921 cgatcgcgtt agctcgaaca cccatcaagt tagcaaacat gccagctaat ccagagccga
    25981 ccattgcagc gacgaaagga tagacgtatt tcaaattgat tccgaacata gcaggttcag
    26041 ttacgcctaa gtaacaagaa atcattgctg gaatcgagat ttgttcttct tttttattcc
    26101 cacgatgtaa gaagatgatg gctaagactg cagagccttg ggcgatattt gataaagcaa
    26161 tcatcggcca taaattcgtt gatccgaaat ctgcaatcag ctgcatatcg atggcattgg
    26221 tcatatggtg gagaccagta atcactaaag gagcatacag gaatccgaat acggcactaa
    26281 acaaccagct gatagaagac gtcaatccag tatttacgat atttgagatc cagcttccga
    26341 ctgtccagcc gataggacct aaaataacgt gtgcagctaa aacagttggt aacaatgaaa
    26401 acaatggaac aaaaatcatc gagatcgact gagggatata tttgcggaag aagatttcta
    26461 gatacgctaa cataaaccct gccagcatgg ctggaataac ttgcgcttga taaccaatca
    26521 tctggacttg agcaaaacca aaatcccaaa atggaatatc tgctgcggct gtggaagcta
    26581 cactataagc atttaataat tgcggagaaa ctaacgtaat tcctaaaacg attcctaata
    26641 tttgtgtagt acccatcttt tttgcaatac tccaagtgat acctactgga agaaaatgga
    26701 aaattgcttc acccggtaac cacaaaaagg cattgacacc attccaaaac ttcgagtgtt
    26761 caacgattgt tccaccaaga ctttcaaact ggataccttc aagtacattt ctgaatccaa
    26821 ggatcaatcc accgacaatg attgcaggaa tcagtggtgt aaagatttct gctaaaacgg
    26881 caatagcacg ttgaacggga tgaaggtttt gttttgcagc tgcttttcct tgttctttgg
    26941 aaaccccttc aactccagat actgcaacga agtcgttata aaaagtagaa acatcgtttc
    27001 ctatgatgac ttggaactgt cctgcattcg tgaacatccc ttttacgcta ggtatatctt
    27061 cgatcgcttt ttcgtccgct tttcctggat cattcaggac aaaacgcata cgagtcgcac
    27121 aatgactaac tgcagcaata ttttcttttc cgccgatatc atgtaacagc ttctcggcat
    27181 ctgcttgata ttttgccata tcaataaacc cctttctctt aacttgtcta tgcaagttgt
    27241 ttcttcattt actatacttt ttttcggaga gaattgcaag cgttttatta aaaagtacga
    27301 gagaaaatga tatcgtttta aaaagcttaa aataagcttt tcttaaaaat aggaacattg
    27361 aaatacaaaa agagctatag tatagtgaag aaatgatgga agaacgagtg agggaaaaaa
    27421 catgaataag ttcaatgaaa tttttttaga tttggaacag aaaattttag cgaaagaata
    27481 tcctccgcat tcgcttttac ccagtgagaa ccaattgatc aaaatttatg gtgtttcacg
    27541 agaaacaatt agaaaagcgc tgaatctttt gactacggaa ggttatattc aaaaaaaaca
    27601 aggaaaaggt tcgatcgtac tggatcggaa tcgcttcgac tttcctattt ctggattgac
    27661 tagctataaa gagctacagg agacccaacg tattccaagt gaaacgatca tccatacgtt
    27721 agaagaaaca gaagtgacaa aagcattgaa cgagatcact ggctgggaga tcggtgcgcc
    27781 tgtttggcat ttgatacgcg aaagaaaaat caatggcgaa gtagttatat tagacaccga
    27841 ttatctatta aaggaaatcg ttcctatttt aactccagca caagcacaag gatcgatcta
    27901 tgaatatttt gaaaatgagc tttctttgac gattgcttac gcgcaaaagg agatcacagt
    27961 agaagaagta acggaaatca tgaaaacagc gatggatctt cacgatgaca cttatgcagt
    28021 agtagtcaga agcctcgttc atctggaaga tacccgctgt ttcgagtatt ctgagtcggt
    28081 tcaccgatta gataaattcc gtttcattga gtttgcccga cgccgaaaag tgtaaatagt
    28141 tcaaaagaaa gcaaattgtt ggcatctgtc cactttggcg ctatgatagg atcacaaagg
    28201 gggctttttt aatggataaa ccaataagac cagaaaaaga agaactgaaa gaaaagttat
    28261 cacccatggc ttatgcagtt acacaagaaa atgcaacaga acgaccattt agcgggaaat
    28321 atgatgattt ttacgaaaag gggatctatg tagatatcgt cagcggtgaa ccgttattca
    28381 gctctgcaga aaaatatgat gcaggttgtg gctggccatc attcagcaaa ccaataacga
    28441 aaagacaagt gcgcgaaaaa gccgattttt ctcatggcat gcatcgaata gaagttcgaa
    28501 gcaaagaagc agattctcat ctcggacatg tgtttaccga tggaccagta gatcaaggtg
    28561 gtctacgtta ctgcatcaat tctgcctcac tgaaattcat tccatatgaa aagatggatg
    28621 aattaggtta tggggaattc aaatcacttg tagaataatg gaaatacaca aagagcggta
    28681 caatacgggg atttactcga tcgtccgctc tttttttatt caagcgattg ctatttgaaa
    28741 gaaacgctcc ccagactcct tttttcacaa ggtgaaatgt gctacaataa agggaacgta
    28801 tgtgtgcaaa agaaagagga ctagaatatg tatgaatatc tgacaggtgt ggttaccttt
    28861 atcaacccct attacatagt agtagaaacc aatggtatcg gctaccagat cgcactagga
    28921 aatccttacc gctacagtag caaactaaat caaaaaataa aaatttatgt ccatcaggtg
    28981 atacgtgaag atgcgcatct tctatatgga tttgattcgc tagaagaaaa gcaactgttt
    29041 ttgcgattag taagtgtttc aggaatcggt ccgaaaagtg cccttgcaat catggcaagc
    29101 gaagaccact ccggtttgat tcaagcagta gaatccagtg atgtcactta tttgacgaaa
    29161 tttccaggag tagggaaaaa gacagcacaa caaatgatct tggatctaaa aggaaaattc
    29221 ggtgagctga gtatcgatac accttttagt ctatttgatg aagcaaacac aaaagacgct
    29281 acagctctat ctgaagcaat ggaagctctc tcggctcttg gttacagtga tcgagaaatc
    29341 aaacgtgtag aaaaacaatt aaaagaagtc gataatcaga caacggatga gtatctacgc
    29401 caagcactca agctaatgat gaaaaaataa ggaggagacc aaatgacaga tgaatacttg
    29461 ttatcaccag aatctggcga aaatgaactg agtttggaaa aatctcttcg accacagctg
    29521 ctttctcaat atatcggaca gagaaaagtc aaacaagaac ttcagattta catcgaagct
    29581 gccagaaatc gggaagaacc tttagatcat gttctgttgt acgggcctcc tggattagga
    29641 aaaacgacga tggccatggt cattgctaat gagatgaccg taaatattcg tacgacgagt
    29701 ggccctgcta tcgaacgtcc gggtgactta gtagcaatct tgaatgaact ggaagcaggg
    29761 gatgtcctgt ttatcgatga gatccatcga ttacctagag tggtagaaga gatgctttat
    29821 tcagctatgg aagatttcta tgtagatatc atggtaggac aagggccaac cgcacatccg
    29881 gttcatttcc ctcttcctcc ctttactttg attggcgcga cgacacgggc aggtatgctt
    29941 tctgcacctt tacgagatcg atttggcatc atttctcata tggaatatta tgacgaacag
    30001 gatctgcagg agatcgtttt acgttcttcg gatatttttc aaacagagat cattgaagaa
    30061 ggggcattgg aaattgctcg ccgatcacga ggaacgccta gaattgctaa tcgcttgctc
    30121 aaacgggttc gtgattttgc gcaagtccaa ggagacggga aaatcgatcg tcctatagcg
    30181 gatcaggcac ttactttgct acaggtcgat caggctggat tggattatgt tgaccagaaa
    30241 ttgttgcgaa caatgatcga gttatatggc ggcggaccag tagggttaag tacgctttca
    30301 gtaaacatag gagaagagcg tgagacggta gaagatatgt atgaaccgta tctgatccaa
    30361 aaaggatttt taaagcgtac acctagaggg cgaattgcta ctgcttatgc ctatgaacat
    30421 tttggttacg actatcatat atgatttttg cttttagatt ggaggaaaat gaatgagtga
    30481 tacaacacgt acgaaacaat tgatcaaaaa cgtattgatt gatttgtgcc aaacgcaatc
    30541 ttttcgaaaa atcagcgtcc aaaaaatcgc aaatgaagca aaaatcaatc gacaaacatt
    30601 ttattatcat tttacagata aatacgattt gcttcgttgg gtctattgtg aggatgcatt
    30661 gcattattta gatccacaat gtctttcact tgataattgg gaagagcaag cattgaaaat
    30721 gctgaaagca attgctgaga agaaagaatt ttatgcaaat actgtgcttt ctgacccaga
    30781 aattttacaa aaagagtttt ctcaactgat ccaaaaatca tttatcaaga tttttgaaca
    30841 aatggatgaa gaaagacaac tttctgcaag agacaaacta ttttatgctc gttttttttc
    30901 ttacggttgc agtggtgtat tagtcaattg gatcacgaat gaatatgaag aaacaccgat
    30961 ggaaatcgct atgcagcttt tccgtttagc caaggatacg gaattatatt cttatcgtct
    31021 ttatgcaaaa gaagagttgg aagaacagta atagaaaaat tttttgttgg aaaagcaatg
    31081 atcgctaaaa ggagatgggg aaatgacaaa aattttattt gtttgtttgg gaaatatttg
    31141 ccgttcaccg atggcagaag gcttgctgag aaaacgaata gcattagaag gaagggaagc
    31201 agaatttttt gtggattctg ctgctacaag cacgtatgaa gttggaaaga caccgcaccc
    31261 tggaacgaaa aaaatattaa accaagaaaa agtagatatg acgaatatgg ttgctcggca
    31321 aatcacaccc cacgattttg aaacttttga ttggatcatt ggcatggatc aagaaaatgt
    31381 agaagaactg aaacgaagag caccacgctc agcgcagggg aaaatccatt tgtttttaag
    31441 tagcattcct ggaaaagaaa aagaaaatgt tcccgaccca tactatacca ataactttga
    31501 tgagacatat catttgatca cagaaggcct gaatcattgg tggaatattt ggattggcga
    31561 aaataaaata acaaacaatt tgtaaagttg acgctttctt tttaaggcga tgttagagca
    31621 tttccttgtt tttttgtaaa atgttaggta aaataaaatt aattaagtaa cactttctta
    31681 gctaaaggag gagtgcattc gatgaacgaa gagacagtat cgccaatgaa gaagcttacg
    31741 aagttagtta ctcatacttc gtatcaactt tttgattctt ttgtaaatga cactgaagaa
    31801 catgctaaga atgtacgatt gcaaggaaag tttattcttg agctgcaaaa agctgctact
    31861 gaacatagtc tagttatctt acaagtaaga gcaaatcgtt tgagtagcaa gtatgaaacg
    31921 atctcaggct gggtggcgac aaaaacggtc gaaaaagacc gagtgatgat ccgcctacaa
    31981 aacgaagagc agcagatccg gatggttcct gtagaacaaa ttgaaaaaat aacaatcctt
    32041 tcaccagcag gggaacagga acgattatca aaataatgta taccagatag aaaagcacgt
    32101 atgagcttgt accatcaatc attactaatc aaaaataacc gaatgcttta agctgatatc
    32161 agtaaaacgg aacgttttag gatgagtatc acagcatgac agcattcggt tgattagttg
    32221 aatcgattgg atggtacaag tttttttgat aaaaaaaggt cctgctagcc ggggagctag
    32281 caggataagg agttaaaaat gaaaaagtgt tgttagggtt gtttgttggt atgcttatat
    32341 aatagtcatt agttgtgaat attttgtgac gaaagttttc gaaagttgtt aagttttttt
    32401 gcgaaaacta tttttatatc atatatatgt ctaaaatcta tattataaaa gaaatgttgt
    32461 ttgatcagct tctcttcggt attagttcaa aacaaaacgg caaagtatat ggagctactt
    32521 tgccgttttg tttctctagt actcaggtca gtttatagac gctcaataaa cggtgttcaa
    32581 aagccagttt ctcggtatta agtcaaattt atgcaaaaat gtgagaagcc attttcacaa
    32641 aatatttctt aatactt
//
LOCUS       DACCKU010000023        32343 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.023, whole genome shotgun sequence.
ACCESSION   DACCKU010000023 DACCKU010000000
VERSION     DACCKU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 32343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.023"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IVN13_001023"
     CDS             591..1280
                     /locus_tag="IVN13_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011525.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IVN13_001024"
     CDS             1267..2475
                     /locus_tag="IVN13_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7011526.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IVN13_001025"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IVN13_001025"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP7011527.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRSVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4285..4980)
                     /locus_tag="IVN13_001026"
     CDS             complement(4285..4980)
                     /locus_tag="IVN13_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP7011528.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(5070..6170)
                     /gene="ychF"
                     /locus_tag="IVN13_001027"
     CDS             complement(5070..6170)
                     /gene="ychF"
                     /locus_tag="IVN13_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP7011529.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(6179..6370)
                     /locus_tag="IVN13_001028"
     CDS             complement(6179..6370)
                     /locus_tag="IVN13_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP7011530.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(6391..7281)
                     /locus_tag="IVN13_001029"
     CDS             complement(6391..7281)
                     /locus_tag="IVN13_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP7011531.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(7268..8035)
                     /locus_tag="IVN13_001030"
     CDS             complement(7268..8035)
                     /locus_tag="IVN13_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7011532.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(8047..8760)
                     /gene="rsmG"
                     /locus_tag="IVN13_001031"
     CDS             complement(8047..8760)
                     /gene="rsmG"
                     /locus_tag="IVN13_001031"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP7011533.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(8992..10893)
                     /gene="mnmG"
                     /locus_tag="IVN13_001032"
     CDS             complement(8992..10893)
                     /gene="mnmG"
                     /locus_tag="IVN13_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP7011534.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(10910..12307)
                     /gene="mnmE"
                     /locus_tag="IVN13_001033"
     CDS             complement(10910..12307)
                     /gene="mnmE"
                     /locus_tag="IVN13_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP7011535.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            12651..12989
                     /locus_tag="IVN13_001034"
     CDS             12651..12989
                     /locus_tag="IVN13_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011536.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(13157..13915)
                     /locus_tag="IVN13_001035"
     CDS             complement(13157..13915)
                     /locus_tag="IVN13_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7011537.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(13933..14751)
                     /locus_tag="IVN13_001036"
     CDS             complement(13933..14751)
                     /locus_tag="IVN13_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7011538.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(14748..15104)
                     /gene="rnpA"
                     /locus_tag="IVN13_001037"
     CDS             complement(14748..15104)
                     /gene="rnpA"
                     /locus_tag="IVN13_001037"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP7011539.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(15222..15356)
                     /gene="rpmH"
                     /locus_tag="IVN13_001038"
     CDS             complement(15222..15356)
                     /gene="rpmH"
                     /locus_tag="IVN13_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP7011540.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            15879..17213
                     /gene="dnaA"
                     /locus_tag="IVN13_001039"
     CDS             15879..17213
                     /gene="dnaA"
                     /locus_tag="IVN13_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP7011541.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            17411..18541
                     /gene="dnaN"
                     /locus_tag="IVN13_001040"
     CDS             17411..18541
                     /gene="dnaN"
                     /locus_tag="IVN13_001040"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP7011542.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            18770..19012
                     /gene="yaaA"
                     /locus_tag="IVN13_001041"
     CDS             18770..19012
                     /gene="yaaA"
                     /locus_tag="IVN13_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP7011543.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            18999..20123
                     /gene="recF"
                     /locus_tag="IVN13_001042"
     CDS             18999..20123
                     /gene="recF"
                     /locus_tag="IVN13_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP7011544.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            20120..22066
                     /gene="gyrB"
                     /locus_tag="IVN13_001043"
     CDS             20120..22066
                     /gene="gyrB"
                     /locus_tag="IVN13_001043"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP7011545.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            22086..24557
                     /gene="gyrA"
                     /locus_tag="IVN13_001044"
     CDS             22086..24557
                     /gene="gyrA"
                     /locus_tag="IVN13_001044"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP7011546.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            24793..25092
                     /locus_tag="IVN13_001045"
     CDS             24793..25092
                     /locus_tag="IVN13_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP7011547.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            25142..25654
                     /gene="ssb"
                     /locus_tag="IVN13_001046"
     CDS             25142..25654
                     /gene="ssb"
                     /locus_tag="IVN13_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7011548.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            25680..25916
                     /gene="rpsR"
                     /locus_tag="IVN13_001047"
     CDS             25680..25916
                     /gene="rpsR"
                     /locus_tag="IVN13_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP7011549.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            26059..28032
                     /locus_tag="IVN13_001048"
     CDS             26059..28032
                     /locus_tag="IVN13_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAP7011550.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            28038..28490
                     /gene="rplI"
                     /locus_tag="IVN13_001049"
     CDS             28038..28490
                     /gene="rplI"
                     /locus_tag="IVN13_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP7011551.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            28764..30131
                     /gene="dnaB"
                     /locus_tag="IVN13_001050"
     CDS             28764..30131
                     /gene="dnaB"
                     /locus_tag="IVN13_001050"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP7011552.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            30911..32203
                     /locus_tag="IVN13_001051"
     CDS             30911..32203
                     /locus_tag="IVN13_001051"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP7011553.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT         9937 a         6241 c         6424 g         9741 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gagcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctttg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca agacttttgt cacagtccct
     4201 tactattatt taactatgga taaacaaaat gaggttcttt actaccccta gatatctagc
     4261 aaaatagctg atctttttgc tagtttatct tcttgggacg gataaacttc caataatccc
     4321 gtattttttc ttcaagtagt aacggattgc taaagcagtt gccccgataa tgataactac
     4381 tactggatct aatactggat tgataactgg tggcaatagt gttgtcgcag aaaacagtac
     4441 gacccaaaga agtgtcgttc cacctaggat aagcaatgtc ttacccattc ctggtttctt
     4501 gcttcgatct gctccgggat attcatattg atagatgtat ttgtacatca tataaaagac
     4561 ccagccaccg attgcagaac ctagaacgag tgtagaaaag ccataagctg gcgttgttcc
     4621 acgtgaaaac aatccggtaa tcgcaatcat gatacttaat agaccaagca acagcaacgt
     4681 attatctaac cacatcaata aaggggtgga tgaatttttt acttctgctg gcttattgat
     4741 gattgcttcc gttctttcag agactgtccc aaacaattga cgggctgttc ttccgccttt
     4801 ttgttctgca actaaagtag gcagaatgtc atgaagggct tttacttttt cttcttcaga
     4861 caaattcgct gcttctaaag attttttcaa atcaaaaatg tactgttgat ttcttttggt
     4921 taattttgtt tctaaactat tgttttcttt tacgattgct cgtaattgtt ctggttccat
     4981 tatctaatag tcctccttag ccgttccttt tctcaacggt cgtcacaaaa gacattctac
     5041 catattctac agctagtttg atagcccttt tacacattaa agcggaataa cattacgtcg
     5101 ccgtcttgta cgacataatc ttttccttct aatcgaacac gtccagcttc ttttgctgca
     5161 tgcatgtttc cgtaatgatc caagtcttca aaagagacag tttctgcacg gataaatccg
     5221 cgttcgaaat ctgaatggat gattcctgct gcttgtggtg ctttgattcc ttttttgaat
     5281 gtccaagcac gtacttcttg ttccccagca gtaaagtaag tagctaagcc taacaaatca
     5341 taagcagcac ggatcaactg atccaaacct gattcttcga ttcctaaagc ttctaaaaat
     5401 tccgctttgt cttcttcatc taattctgcg atttcttctt ctgcacgagc actgaccacg
     5461 atcacttctg cattttcttc tgcagcaaag ttgcgcactt cttgtacata atgattcgcg
     5521 tctgcatctg ctacgtcatc ttctgagaca ttggctacat aaagaattgg ttttgtcgtc
     5581 aataaaaata gtgatttaac gattttttgt tcttcttcag taaattcgat cgttcgggca
     5641 gattttcctt cttccagtac aggtttgatt ttatccagta cagctagttc tgccactgca
     5701 tctttatctt tggttttagc aacttttgct acacgagtat agcgtttgtt gatagattct
     5761 aagtctgcta acactagttc caagttgatc gtatcaatat cttctagtgg atcaacacgt
     5821 ccttctacgt gagtaatatt atcatcatca aaacagcgaa caacgtgaca aatagcgtct
     5881 acttggcgga tatggcttaa gaattgattt cccaaacctt ctcctttact tgcgcctttt
     5941 acgattccag caatatctgt aaattcaaaa gtcgtcggaa cagttttttt cggatgtaca
     6001 agttctgtta agcgttgcaa acgccagtca ggtacttcta ccatccctac gttggggtcg
     6061 atcgtcgcaa agggatagtt tgctgcttct gctcctgctt ttgtgatcgc attaaacaaa
     6121 gttgattttc ctacgttggg caacccaaca attccagcag tcaatgccat aaatttattc
     6181 actctttctc tgcatttttt tcaattactt tttttaattt cttttcaaaa tctcgacggg
     6241 tcatcatgac gatatgtccg caattcatac atctgatttt gatatctgcg cccattcgaa
     6301 tgacttccca gcgattcgtc tggcaagcgt gaggtttttt catttcaacg atatcgtgca
     6361 agtcgtacat aaaaatagcc tcctttttgt ttattcttca tcaaattgaa tattcaagat
     6421 atcaagaatt cgagtcaaat ctttttgaga aagatattca atctcgatct tacctttccc
     6481 atttttttct tggatagcta cacttgtacc aaatttatcc atcaaacgat cttcgctttc
     6541 tcggatataa tatggttttt cctttgctag ccttgggatt tttttctttt cttttccatc
     6601 attttgattt aactcagaaa caagctgttc cagttggcgg acagttaaat tttcagttac
     6661 tgcacgattg gctaatttca agatcaatgt tttatttttt aaacctaaca gcgtacgggc
     6721 ttgtcccata gataaacgct gatcttgtac catttccttg acaagatccg gtaaagaaag
     6781 caagcgcaaa taattggcga tataaggtct gctctttcct aaacgactag ctacttctgc
     6841 ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct tctgcttctt ctaacggatt
     6901 cagatcttct cgctgcaagt tttccagcac tgctacttgc atcatcgctt cttcgtcaaa
     6961 gtctctaacg atagcaggga tcgtttcttt ttcagctagt ttggacgctc gaaaacgtcg
     7021 ctcccctgca ataatctcat atccttttac tgcggaacgg cgtacgatga tgggttgaaa
     7081 gacacctgat tgttcaatag aattagctag ttcctgcaaa gatacttctt caaatgtttt
     7141 acggggctga tacggatttg gtcgcagctc attcaatggt agctggacaa cggtatcatc
     7201 ttttacatcc accgtctcta agttttctaa atcttgaaac agtgcatcga ttcctcggcc
     7261 taatccttta cttttcttca cgacttacca cttcctttgc caatgcttgg tagacttcag
     7321 ctcctcgaga acgtggatcg tagtcgatga tcggttttcc atgactaggt gcttctgata
     7381 aacgaatatt tcttggaatg atcgtttcgt aaactttttc gcggaagtat ttacgtactt
     7441 cttctacgac ttcatttccc agatttgttc gtgcatcata cattgtcagt aagactccct
     7501 cgatctctaa ttctggatta aagtgttttt ggactaaacg aaccgtattc agtaattggc
     7561 ttaatccttc caatgcgtaa tactcacact gaacaggtat caaaatcgaa tcacttgctg
     7621 taaatgaatt gattgtcaga tggccaagag aaggcgggca atcgatcaaa atataatcat
     7681 actgactgct tacttctgct aaagatcctt ttaatcttga ttctcttgcc atcattgacg
     7741 tcaattcgat ctctgctcct gctaattgga gagtcgcagg cacaatactc aaattttcgt
     7801 gttcagtgat caaagttgcc tcatcaattg gcaattcatt gactagaaca tcatagatgt
     7861 ctcttgtcac atcaggtttt ctgatcccga caccactggt agcatttcct tgtgcatcca
     7921 tatccactag caatactttt ttgccaaggc tagcaagaca agcacctaga ttcaccgtcg
     7981 tcgttgtttt gccgacgcca cctttttggt tcgcaacaga tattatttgt gccatctctt
     8041 ctcctcctat ttgattggct gcttattagg cagtccgggc tttcttgggt atttctttgg
     8101 ggttggtttc tttttttgaa taacgatgat atgccgttga tcagagacat tcggcaaggt
     8161 aaccgcctct tctttgagga atttaccacc taataaagca atcgcttgtt ttgcttctgt
     8221 caattcctct tcactttttg ctgctttcaa tgcataaaaa tagccttctt gtttagccaa
     8281 gggcaaacac aattccgcta acacattcaa acgagcaacc gcacgagcag tgacaaggtc
     8341 aaatgatgca cgaaagtgag gattttgccc aaatgtttct gcccggtcat gatataagga
     8401 agcatcgatt cctaataatc gggttaactc agataagaag gttattcgtt tattcagaga
     8461 atcaacgatc gttatctgca gttctggaaa ggcaattttt aaaggtatgc taggaaaccc
     8521 tgcccccgaa ccaacatcgc ataaagataa aggttgcgta aagtctactt caaatgctaa
     8581 tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt tcatccgtaa tagctgtaag
     8641 gttcatcttt tcgttccatt cgactaatgt ccgataataa atagcgaatt gattcatttg
     8701 atgatcggta agctctatcc ctttttctgc caaggattgg cgaaattctt ctggtatcat
     8761 aaatattcct ttcattgtga aacagcaatt gtttcacaga ttctctccct actttaacgc
     8821 aaaatctcta gacatgcaat ccttccatat ggattcttaa aaatttgaaa agtaagaaaa
     8881 gccgatagac tttttctttc tagtatgact cataaaatat acacagaaga gagaactagc
     8941 tttgagcaat ttctgcaaaa actagttctc tcttctattt attattacgt attacgctgg
     9001 ttcactgact ttcgcaatct tcccttgttc gatatagacc attaggatac ttaggtctgc
     9061 agggtttacg ccactgattc ggctagcttg ggcaattgtt tctggttgga ttttctgcaa
     9121 tttttgtttt gcttctgttg ctaatccgtt gattgctgcg tagtcgatgt tttctggaat
     9181 ctttttcgct tccatgcgtt ttaatttttc tactttttcc atggcttttt tgatgtagcc
     9241 ttcatatttg atttggattt ctacttgttc gatttctttt cgtgtcaatt cttcattttc
     9301 tggaataaac cgtagaagtt ctgcataagt aatctcagga cgtcttaaaa agtcgcttgc
     9361 tagtacacca tcttttaatg gtgccgcatt aatcgttgct aaaaagtctt ggacttcttc
     9421 tgttggtttg attcgtacat tcatcaaacg tttgatttct tgttcaactg cttctttttt
     9481 cttcaaataa ttcttgtatt gatcttcttt tactaagcct acacggtgac ccatttctgt
     9541 taatcgcaaa tctgcattat catgacgtaa aagcagacgg tattcagcac gtgaagtcag
     9601 caaacgataa ggttcgttcg ttccttttgt taccaaatca tcgatcatga caccgatgta
     9661 accgtcactt cgtttgatca ctaaaggttc ttttccctga actttcaaag ctgcattgat
     9721 accggcaatc agcccttgtc cagccgcttc ttcataaccg cttgtaccat ttgtttgtcc
     9781 agctgtataa agattttcaa cgactttagt ttctaatgtc ggacggagct ggtgtggctc
     9841 tactacgtca tattcgatag catatcctgt acgcatcatt tctacttttt ctaatccagc
     9901 aatagaatga agcatgttga cttgaacatc ctctggtaaa gaagtagata aaccttgaac
     9961 atagacttct tccgtattta agccttctgg ttccaaaaat aattgatgac gtggtttgtc
    10021 tgcaaaacgc acgatcttat cttcgatgga tggacaatag cgagcgccta ccccttcaac
    10081 gatccctgta aacattgggg cacgatgtaa gttgtctcga ataatttggt gagtcgtctc
    10141 acctgtatag gtcagccagc aagattcctg atctagttta taggcactat ctggtgtgct
    10201 gaagctaaaa tgattcggct ctttgtcacc aggctgctct tctgtaacag aatagtcgat
    10261 cgtacttgat tttactcgag gcggtgttcc tgttttgaac cgttcgatag caaagccaag
    10321 ttcttttaga tgattcgcta gtccaataga aggttgagag ttattcggac ctgatgaata
    10381 tttcaattca ccgataatga tttctccgcg aagtgctgtt ccggctgtaa taacaactgc
    10441 tttggcccgg tagctcgctc ctgtggacgt cactactcca tgacaaacac catcttcgac
    10501 gatcagttct tcgacgattc cttgtcttag cgtcaaattt ggttcttttt cgatcgtatg
    10561 tttcatttct gttgaataag catgtttgtc tgcttgtgct cgcaatgctc ggacagctgg
    10621 gccttttcct gtgttcaaca tacgcatttg gatataggtt ttatcgatat tcttacccat
    10681 ttctccgcct aacgcatcga tttctcgaac gacaacgcct ttagctggtc cgcctacaga
    10741 aggattacat ggcatgaatg ctaccatgtc caaatttatt gtcagtaata atgtttttga
    10801 tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg tgtcctgcgc caacaacgat
    10861 cacgtcatac gcatctgctt cataatgttc caatcgtttg ttcctccttt tattttccta
    10921 aacaaaattg actgaataat tgagtgatta gctcatcttg tacactgtcg ccgacaactt
    10981 ctcctagata gtcccagcaa cgcgtcatat ctatttgaac gagatccaca ggcattcctg
    11041 aatcaatacc tgcaatgact tcttcaagtg agagggaagc tttttctaac aacgcgatat
    11101 ggcgggtatt agaaacataa gtagcgtctc tttctcctgt ttctccgctg aagaataaat
    11161 cagaaatagc tgattccaaa cgatctagtc cgtcgttttt agctacggaa attgaaaaaa
    11221 ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc cagctgtgct ggcaaatctg
    11281 ttttatttag aagaatgatc cgtttcaatc cacttgtcgc ttccaataat tgttcatctt
    11341 ctgctgtcag tggttcgctt tggtttagaa caagtaagat caagtctgct tctgctaatg
    11401 cttttcggct tctttcgacc ccgatccgtt ccaccacatc ttctgtttcc cgaattccag
    11461 ctgtatcgat tagcttgagc ggtacccccc gaacattgac atactcttcg attacatctc
    11521 gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc tcttaacaaa tggtttaaca
    11581 aacttgattt gccgacattt ggccggccga tgattgccgt acttaaccct tcccgcagaa
    11641 ccttcccttg cttggatgta gcaagtagcg cttggattct ttgttggatc atctgcgctt
    11701 tttcaagcaa taacttcgta gtcaattctt cgacatcatc atattctgga taatcaatat
    11761 ttacttctac ttgtgctaat gtctctaaaa tctcttgtct caatgagcgg atcaaagcag
    11821 acagattgcc gtctaactga ttaagagcta gtcccattgc tttgtctgtt tttgcccgaa
    11881 tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg accattcaag aatgctcgtt
    11941 ttgtgaattc gcctggctca gctaatcttg caccttctct taacaatagc tgcaaaattt
    12001 gattgactac aacgatccca ccgtgacaat tgatttctac tacatcttcc cgcgtaaatg
    12061 tctttggtgc tctcatcacg gagaccatga cttcatcgac aagctgattg ctcttgggat
    12121 caacgatatg tccataatgg atcgtatgac ttggtacttc tgataatcgt ttgtttccag
    12181 attgataaac tttatctgct agttgcacag cttgatcgcc acttaaacga acgatactga
    12241 ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt atcaaattct aaagtaattt
    12301 cagccatttt ttcacttcct ttttcatttt gcgcacaaaa aaagtgccca ctccaccctt
    12361 tatgcacaat aaacggtgaa cttcgcactt tgactgcttg ttcttgatta aattctggtc
    12421 atagattatc acacttgcta taaggaatca agtgtttaaa acaattagat gaagttgagc
    12481 ctgttctgct tccattacct tatatttaat tgtccttgga tgtctctttg cgttctttgg
    12541 tttattcgaa gagaagatac tgtataaaca aggctttatc tttcttaaaa aaaacggtac
    12601 actaaatggt agcgctatct aaactataca cttttgaaag gagaaaatat gtgagcccgt
    12661 tcagatctgc tgcattggaa gaagaaagtg accctcggat caacgatgtc gttttatcta
    12721 cagggaatat caaccagaaa tatatcgtcc gcgatatcgt ttttgttgtg gaatgcttag
    12781 aagctgattt gtttgaccca tcttttccca aagatgacct tttcttaaca acgaagagga
    12841 aattaaagac gaaggtactt gaatatggtg cgaatgcagt aatcaactgt cattttgagt
    12901 atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact ttttgcttat ggaacagtga
    12961 tccaatttat ccaaacaacg attggttaaa tgaaacagac aaaaaaagcg gatcaaaact
    13021 gaagacagtt tcgatccgct tttttctatc aaagttagtg actacttcat cgtatactct
    13081 gataagaatg atttctagta aaatatactt ggtttgaaaa actttgtcaa cgtgatttat
    13141 tcaaaaagta atggaatcaa taatattttt tagcaggttc tactactaaa taacgataag
    13201 gttcctcacc ttccgaatgt gtttttacat gttcatcttt gcttaatgca gcatggattt
    13261 gtttacgttc aaatgcaggc attggttcta agaaaacagg ctgccctgtc cgttttactt
    13321 tttcagccgt gcgatcagct aaacgctgaa tgatctcttg gcgcttttca cggtaattgc
    13381 ctacattgac tactacagac agcttatttc ttgctaaacg atggatgaag acttgtgcca
    13441 gatattgcaa tgcattcagt gtttttccat gtttcccaat cagtatgcct tgcttttgtg
    13501 tttctaaatg gaaaacaacc atattatttg attctctttc cattttgaca agagctggag
    13561 catcaagctg ttttgaaatt tcagtaagat aaactgccaa ttccgttaaa gctgcttcat
    13621 cacctagctc acttacttct tctgtttttt cttcaagaat cgtttcgttg actacctcga
    13681 tctcagctgt tggttcttct tccaaaattt cttctacagt ctgttctact gtttcagaaa
    13741 ctttttcgct gatacttggc tggatcgaaa gccgtgcatt ctttcgaccc attcctagga
    13801 aacctttttt tccttcttcc agtatttcga tttcgacttg gtctttggcc aaacccaaat
    13861 cagccagacc tagttggaca gcttcgtcta ttgttgcagc ttcataaatc ggcattagca
    13921 ttacctcccc tattatttcc gttttttctt aggattcatt gctttcttaa gagcacgttc
    13981 tctttcgcgg attctccgtg cttcttcttc acgctctttt cgaattttga aagggttgtt
    14041 gatcagcagc gtttgtacaa cttggaaggc attggaaact acccagtata atgaaagacc
    14101 gctggctaaa ttgacaccca tcactaaaat cattacaggc atcacgaaat tcatgatctt
    14161 cattgatgca ttcgattcaa tttgactcat gctagataaa tacgtacttg caaaagtaaa
    14221 gattgctgct aaaatcggca aaataaatgt tggatccgcg ttccctaagt tcaaccataa
    14281 gaaatgccct tgtttcaact ctggtacacg tgaaatagat tgccataacg ccatcaagat
    14341 tggcatttgt actaataaag gaagacaacc agcatatgga ttgacgccat tttctgcata
    14401 taaacgctgc tgctcttctc ttaacttact ttgtgtttcc ggatctcttg aactatattg
    14461 ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt ttacgcatgc tctttgtttg
    14521 gaaatgcatc aacggcaata ggatcacgcg gatgatcagt gtaaataaaa tgatccctat
    14581 ccctgcatta ccaaatgaca acgccttgat cgcctcagca aagtaataaa caatataacg
    14641 atcccaaatt cctgtactct gtgcgctgac ttccccagtt ccgcaaccgg ataagacgat
    14701 gaccaaacta acgataccgg ccatcaaaag taaacgtttg tatttcttca cttactcttt
    14761 tcctctctgt cgattatttt tgcaagtttt aagacgtgga ctaaattgga gcgaacctca
    14821 tcataagtca accccttcac acttggtcta gcaatcacaa taaaatccaa ctccggatca
    14881 atttcttctt ttatagaata aactgctgct cggatttttc gtttgacttc attgcggttt
    14941 accgcgttgc cgaccttttt tcctacagat aaaccaactc gaaaatgagg ctgtcccggt
    15001 ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag aatctcccgt atggaacacg
    15061 gcttgaaact ctcgttcttt ttttactcga tacgcttttc tcattagttt tctttccttt
    15121 tctatctctc acttacgctc ataatcatac cataattacg attgtccttt catcatttcc
    15181 ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg cctaagctga aagaactttt
    15241 cttcctttgc gacgacggct agctaacaca cgacgtccat ttttagtact catacgttta
    15301 cggaatccgt gaactttttg acgtttacgt ttatttggtt ggtatgttct tttcatatat
    15361 tccacctcca aaatgttctt atctatttta taaacacaga catactttga tagtataacg
    15421 acaaagtagt gattttgtca atataaaata gaagatcgat tgttaaaact tatccacaag
    15481 attatttttt tctatgggaa tcctatgtgg aaaaagttat gcacagggat tatccacaga
    15541 taataaaagg ctggttttac acagaaaaca cacgtgtaaa aaaagtaatc cacaatcagg
    15601 cgatttcctg tgtattccgt ctcctaacta ttggtatatc tgtatttttt atccacagac
    15661 gcaagaggaa atctattggt ttttacgagt tttccacctg tgcattagtg gtgtggaaaa
    15721 ctttttttga caactgtgtt tttctctttt ttctttttgt cgattgtgtg gataattgta
    15781 attaaaaaat ttttttcttc ggtttctgtg gaaaactatt gaaaaaaatg ctaaattagt
    15841 actagttatt cactttttaa tgaaggagga ggccttatat ggtatccctc gatgctttat
    15901 ggaatgaatt aaaagcaaca taccaaaagg atttatcgcc tgctagctat aatacatgga
    15961 tcgaaacagc gaaccctcga acccttgatc aaaaccagct cgttgttgag gttcctagca
    16021 agattcataa agaatattgg gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata
    16081 tgttatcagg aaatgagatt atccctcgct ttattactgg cgaagaagca gagcaagaag
    16141 aagttataga agaaaaaaat ccaaaagttg tggcacctag tccgctaaaa aaagccatgc
    16201 tgaaccctaa atataccttt gatacgtttg tcattggtaa aggaaaccag atggctcatg
    16261 cagcagcgct cgttgtagca gaagatccag gttctattta taacccgctc ttcttttatg
    16321 gaggcgtggg acttggtaag actcacttga tgcatgcaat tggtcatcaa atgctgcaaa
    16381 gtcagcctaa tgccaaagta aaatatgtaa gcagtgaaac ttttgctaat gatttcatca
    16441 attcgatcca aaacaaaaca gcagaagagt tccgtcaaga atatcgaaat gttgatttac
    16501 tgctagtgga tgacattcaa ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc
    16561 atactttcaa tgccttgtac aatgaaggga aacaaatcgt actaaccagt gatcggttgc
    16621 caaacgagat tccgaaactg caagaacgcc ttgtttctcg ctttgcatgg gggctgtctg
    16681 ttgatattac accgccggat cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg
    16741 ctgaacgtct agagatccca gatgacacac taagctacat cgcaggacaa attgattcta
    16801 acattcgtga attagaaggt gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg
    16861 aagatatttc gactagtcta gctgcagacg cactgaaaac gttaaaatca ggaaaaggac
    16921 atcctcaatt gtcaatcctg cagatccaag aagaagtggc aaagtattat cacatccaat
    16981 tgaaagatct caaaggaaag aaacgcgtca aatctattgt tgttcctaga caaatcgcta
    17041 tgtatttggc acgtgagctc acagataatt ctttaccgaa aatcggagct gaattcggtg
    17101 gcaaggatca tacaacggtt atccatgctc atgaaaaaat ccagcagctg atggattcta
    17161 gtgtcagtat gcaaaatgaa gtatcagaaa tcaaaaactt tttactaaat taatttgtcg
    17221 attctatcaa ctgaaaatag catgtggata aaagaaaaaa acgttcaaaa gttatacaca
    17281 ctttttccac agctgttttt tcgtttctct cttacgtttt ttgagttttc cacagaatca
    17341 acatgcccta ttacttttat tattcttttt aactaataat aaataaacaa acattcaaag
    17401 gagatacatc atgaaagtta ctttaaaccg agctagcttt atgcaggaat tgcaaactgt
    17461 tcaacgagct atttcaagca aaaccacgat ccctattttg acaggtgtaa aaatcacact
    17521 gacacaagaa ggtttgactt tgacggggag caacgctgat atatcaattg aaactttttt
    17581 gtctgttgaa aacgaaaaag caaatatgca aatcgaatct actggttcca ttgttttaca
    17641 agcacgtttc tttagcgaaa tcattcggag acttcctgaa gaaacattta ctttagaagt
    17701 tttagaaaat aaacaagtag cgatcacttc tggaaaagcg aattttatcg taaatggatt
    17761 agatgcagat aactatcctc atcttcctgt tgtcgaaagc cataaccaga tgaaattacc
    17821 tgtacacgta ttgactaaac taatcaacga aacagttttt gctgtctcac aacatgagag
    17881 tcgtccaatc ttgacaggtg tgcatttcat tttatctgat aattctttat tagctgtagc
    17941 tactgattct caccgtctaa gtcaacgcgt gattccagta gaacaagcgg ctgatcattt
    18001 tgatattgtt attcctggaa aaagtttgat cgaattatct cgctcattaa caaatgaaga
    18061 agaaatcgtc gaaatcagca ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta
    18121 tttctattcc cgtttgctag aaggaaacta tccagatacc aatcgtttga ttccttcaag
    18181 ttttaataca gaggttgaat tttctgttcc tagcttttta gcggcgatcg aacgggcttc
    18241 tttactttct catgaaggcc gtaacaacat cgttcgttta tctattcgtc cagatgcggt
    18301 tgttttatac ggaaattcgc cagaaatcgg gaaagtagaa gaaagcttga gttatacagc
    18361 aagcagtggc gatccattag atatttcttt caatccggat tatatgaaag cagcgttgcg
    18421 tgcatttgga gatatgagca tcaaagtgaa atttatctct gctattcgtc catttacatt
    18481 ggaaccaacg gaagatggcg ttcaatttat tcagctgatt actcctgtac gtacaaatta
    18541 acaatgattt tcaatgttct tgaaaattta attgaaaaga aatagaaaaa ataaaatttt
    18601 aaacagaagg ccctaaaatc tcttttaagg ctttctgttt atttttgttt aaatcacctt
    18661 ttttagatta aatcgtctta tatggaaaaa tatatggata aatttgtttt attgcgaaaa
    18721 aaaaggtata atagagtata cttttaataa tgaaaatgag ggatgggcat tgaaacaaca
    18781 agtcattcta aatacggaat tcatgacgtt gggccaaatg ttgaaagaag tcacagttat
    18841 cggtagtggc ggacaagcaa aatggtattt ggcagaaaac actgtgttag tcgatggcga
    18901 accagaaaat cgtcgtggac gcaaacttta tcccggcatg atggttgaag ttccagaagt
    18961 aggaactttt tttatggtaa aaaaaggaaa cgacgataat gaggctgaat aagctgtatt
    19021 taaaaaatta tcgaaattac gaagagctga acatcgaatt ttccaagaac ctcgtcatct
    19081 ttttaggaga aaatgcgcaa ggaaaaacga atcttttgga aagtatctac gtattagcta
    19141 tgacgcgaag tcatcggaca agtagtgagc aggaattgat tggctggact gacgatcagg
    19201 cgatgattca aggagagatc acaaaaggtt catccactct tccattagaa atcttattat
    19261 ctaaaaaagg ccgaaaaacc aagatcaacc atatcgaaca gaaaaagtta agtacttacg
    19321 tgggacaaat gaatgtcatt ttatttgcac cagaagatct ttctttagta aaaggcagcc
    19381 cgcagaccag gcggaagttt ttagatatgg aattaggcca gatcaatcca atctatcttt
    19441 atgatcttgt ccaataccaa gcaattttga aacaaagaaa ccagtacttg aagcaattga
    19501 atgaaaaaaa acaaactgat ttgctttatt tggatatttt gtccgaacaa ctagcagcgt
    19561 ttggcagcaa agtattgaaa gcaagagcac agttcgtcaa gcgattggag ttttgggcta
    19621 attctttgca tcagcaaatc acccatcaaa aagagcagct ggaaatcgag tatctcaccg
    19681 cagtcgattc tttagaaaca catacgcaag agcaaatcca agaacaattt ctcgcacttt
    19741 tgaaccaaaa taaaaagaaa gacctttttc ggggaacaac gacagttggt cctcatcgtg
    19801 acgatctttc cttttttatt aatcagaaaa atgtgcagac ttatggatcg caaggacagc
    19861 aaagaacaac tgcactaagt gtcaaattag cagaaatcga tttgattaaa gaagagacag
    19921 gggaatatcc aatccttttg ttagatgatg tgatgagcga gctggatgat aatcgtcaac
    19981 tccatctgct agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg
    20041 accatgtaaa aaataaaatg actgttgaac ctgagatttt ttatgttcaa caagggaaac
    20101 taaaaggaga agaaactgaa tgacagaaga aagaagttta gtagaacgcg ctaaagagta
    20161 tgatgccagt cagattcaag tacttgaagg acttgaagcc gttcgtaaac gacctggcat
    20221 gtatatcggt tcgactagtt ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa
    20281 ttcgatcgat gaagtattag caggttttgc aacgaaaatc catgtgatta ttgaaaaaga
    20341 caacagtatc accgttattg acgacggtcg ggggatacct gtagatatcc aggcaaaaac
    20401 tggacgtcct gcagttgaaa cggtctttac tgttttacat gctggaggaa aattcggcgg
    20461 tggtgggtac aaagtttctg gtggacttca cggagtagga tcttctgttg ttaacgcgct
    20521 ttctacacag cttgatgtca aagtatacaa agaaggaaat gtgtactatc aagaatatcg
    20581 tcgtggagct gtagtggacg acttaaagat tatcgaacaa acggatcgcc atggaacaac
    20641 ggttcacttt acgccagacc ctgaaatttt tacagagaca actgagtttg atttttcaaa
    20701 attggctacc cggatccgtg aacttgcttt cttgaaccgc gggatgcgta tatcgattga
    20761 agacaaacga gaagaagaac cagtcatcaa tgaataccat tatgacggcg ggattaaaag
    20821 ttatgtcgaa tacttgaatg caaacaaaac cgtgattttt ccagaacctg tatatttgga
    20881 aggtgaacag caagatatcg cagttgaagt ctctatgcaa tatacagacg gctaccattc
    20941 caatatcatg agttttgcca acaatatcca tacgtatgaa ggcggaacac atgaatcagg
    21001 attcaagact gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa
    21061 agaaaatgac gacaacttga ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc
    21121 tatcaagcat cctgaccctc aattcgaagg acaaacaaaa acaaaattgg gcaattccga
    21181 agtacggaca gtcactgatc ggttatttag cgaacatttc atgaaattct tgctggaaaa
    21241 cccaagtgtg ggccgtcaaa ttgttgaaaa aggattacta gcttccagag ctcgtctagc
    21301 agcgaaacgt gcgcgtgaag taacgagacg taaaggtgct ttagagatta gtaatctgcc
    21361 aggaaaatta gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga
    21421 aggagattcg gccggcggat cagctaagca aggtcgtaat cgagagtttc aagccatctt
    21481 gcctattcgt gggaaaatct tgaatgttga aaaagcaagc atggacaaga tcttggcaaa
    21541 tgaagagatc cggtctttat ttactgctat gggtacaggg tttggagaag attttgatgt
    21601 atcgaaagct cgctaccaca aattagttat catgactgat gccgatgtcg acggcgcgca
    21661 tatccgtaca ttgctgctga ctctttttta tcgttacatg cgtcctattg ttgaagctgg
    21721 atacgtctat attgctcagc cacctttata cggggtaaaa caagggaaga atattacgta
    21781 tgtacaacca ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc
    21841 ctcaccaaaa ccaagcgttc agcgttataa aggacttgga gaaatggatg atcatcaact
    21901 atgggaaaca actatggacc caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc
    21961 tattgcagca gatcagatat ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc
    22021 attcatcgag gaaaatgcac actatgtgaa gaacttagat atttaatcat gaaggaggaa
    22081 aaagaatgag tgaagaaatc agagaaaaca tccatgatgt caatctaacc agcgaaatga
    22141 aagactcctt catcgattat gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca
    22201 gagatggtct gaaacccgtt catcgccgta ttttatacgg catgaacgaa ttaggtgtaa
    22261 caccggataa agcccataaa aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc
    22321 atcctcatgg agactatgcg atctatgagt caatggtacg gatggcacag ccattcagct
    22381 accgttatat gctagtggat ggtcatggaa actttggttc tgttgacggt gacggtgccg
    22441 ccgctatgcg ttataccgaa gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata
    22501 tcaataagaa cactgttgat ttccaaagca actatgatga tacagaaaaa gaaccagttg
    22561 tactacctgc tcgcttcccg aatcttttag tgaacggaac aacggggatc gccgtaggta
    22621 tggccacgaa tatcccacct cataacttgt ctgaggtagt agcggctatc gatttgctga
    22681 tggataatcc tgatgtgacg accaatgaat tgatggaagt attacctgga ccagattttc
    22741 caacaggtgg tttggttatg ggaaaatcag gaatccgaag agcgtatgaa acaggtaagg
    22801 gttctattac cgtacgtgca aaagttgaga ttactgagat gccaaatggt aaagaacgga
    22861 tcatcgtgac agaattgcct tatatggtca ataaagcaaa attgatcgaa cggatttctg
    22921 aactgcacag agacaagcgt atcgaaggga tcacagattt gcgggacgaa tcttcccgtg
    22981 aaggtatgcg gatcgtcatt gacatccgtc gtgacgccag cgcatctgtt atcttgaata
    23041 acttatataa attgacatcc ttgcaaaatt catttggatt caatatgttg gcgatcgaaa
    23101 aaggtgtgcc gaaagttttg agcttgaaac aaattcttga aaactatatc gaacatcagc
    23161 gcgaagtcat cacaagacgg acagtctttg aaaaagaaaa agcagaagca agggcacaca
    23221 ttttagaagg tttgcgtatt gctttggatc atatcgatga aatcattgcg attatccgcg
    23281 ggtctaaatc agatgacgaa gcaaaagcga cgatgatcga acggttcgat ttatccgatc
    23341 gccagtctca agctatttta gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa
    23401 aaatcgaaaa tgaataccaa gaactcttaa aactgattga agatttaact gacattctgg
    23461 caagaccaga acgtgttaca gaaatcatca agaatgaatt agcagacttg aatcaacgat
    23521 ttggtgataa acgacgcaca gaattactag tcggtgaggt cttaagtctg gaagatgaag
    23581 atttaatcga agaagaagaa atcgttatta cattgacaaa caatggttac atcaaacgtt
    23641 tggcaaataa tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta
    23701 tccacaatga cgacttcgtc aaaaatctgg tttcttgttc tacccatgat acattgttat
    23761 tctttacgaa taacggaaaa gtgtatcggg caaaaggata cgaaattcca gaatacgggc
    23821 gcacagccaa aggaatacca attatcaatt tactaggtat cgattcgagt gaaagcatcc
    23881 aggcaatcat tgcggtgacg ggagaagcag aagaaggcca ctatctcttc tttacgacgc
    23941 gtcaaggtac agtaaaacgt acttctgtca ctgcttttgc taacatccga agtaatggtc
    24001 tgatcgcaat tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg acagatggtc
    24061 aatctaatat cattattgga acacataacg gctactctgt cactttcaaa gaagaagcag
    24121 tccgcgatat gggacgtaca gcttccgggg ttagaggaat acgcctgcgt gaggaagatt
    24181 atgtcgttgg ggcagcctta atgaaggaag atcaagaagt attagtgatc acagaaaaag
    24241 gttacggaaa acggacaaaa gtatcggaat atccagtgaa aggccgcgga ggtaaaggga
    24301 tcaaaacggc gaacatcact gaaaaaaatg gtccactagc tggattgacc actgtttctg
    24361 gagatgaaga catcatgctg attacagata aaggtgtcat tatccgtttc aacgtatcga
    24421 ctgtttctca aactggacgt tcaacattag gcgtacgttt gatgaagatg gaagctgata
    24481 cgaaagttgt cacgatggca gcagtcgaac cagaagctgc agaacaaaca gttgaagaac
    24541 aaggatcaga agaatagcca tcaagactaa ttgtgtaagc tagcaagaat gaccgattaa
    24601 aaaatctggt tattcctgct ggcttttttg attagttgaa tttctcttaa aatattcgaa
    24661 actgttcatt ctgtattgct tttgaccaaa atagagtgta taatcagagc ttgtgagtag
    24721 tctaattaat tagataactc tccttgctct taggaaccta agagccgaaa gtccacaggg
    24781 aggtgaatat caatggaaaa cacgaaatat gaaatcatgt acattattcg tccaaacatt
    24841 gatgaagaag caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa agataacgga
    24901 gcagaagtta tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga aatgaacggt
    24961 taccgtgaag gtatctatca catcgttaaa gtatcttctc catcatcagc aaatgctgtc
    25021 aatgaatttg accgtcttgc taaaatcaac gacgatatca ttcgtcacat gattgttaaa
    25081 gaagaagctt aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg agatgactat
    25141 tttgattaat aatgttgtat tagttggacg tctgacgaaa gatccagatt tgcgttacac
    25201 agcaagtggt acggcagtcg caacatttac tttggctgtg aatcgtaatt ttacaaacca
    25261 aagcggcaat cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac
    25321 tttagcaaac tatgctcgta aaggaacact tttaggtgtt actggacgta tccaaactcg
    25381 ttcttatgat aatcagcaag gacaacgtgt atacgtgaca gaagtagtag ctgacaactt
    25441 ccaattgctg gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg gattccaaag
    25501 ttctggtcaa tcagctggcg gatttggcgg aaacaataac tctaatcaaa catctcaatc
    25561 atccaacggt atgcctgatt tcgaccgtga tacttctgac ccattcggct caagttcaac
    25621 gatcgacatt tcagacgatg atttaccatt ctaaacgaat ttgataggag ggaaatagaa
    25681 tggcacaaca aagaagaggc ggacgtaaac gtcgtaaagt agactacatt gctgctaacc
    25741 atattgaata tatcgattat aaagatatcg aattactaaa aagatttatt tcagaacgcg
    25801 gtaaaatctt gccacgtcgt gtaactggta caggcgctaa aaaccaacgt aaaattactg
    25861 ttgcaatcaa acgcgctcgt atcatgggct tgctaccttt cgttagcgaa gactaataaa
    25921 tcctaggaat tgaatgactg atccttttga tcagctattc aattcctttt tttctgtaaa
    25981 aataacctct attcgtttag tcgctcgaat gacgaaagtt atggtaaaat caaattagtc
    26041 atatatggga ggaaaattat ggaaaataaa aacaatcatt tatccccgaa acctctcttc
    26101 cttatcctgt ttatattaat cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt
    26161 agcgcttcgg tcattctatt gatgaattta tggggtgttc gctattggtt gaaaatgaaa
    26221 cgttggctta ttttaaataa acgtgaaaaa atccagcagg cttcgactgt agctgaaacc
    26281 aatctagctt atgtctcaga agtcatgcct gtaggagtga ttgcttatca gccatcaagc
    26341 caaaaaatcg aatggctcaa tccattagca ttgaaaatca aagatcaaag tggattaaca
    26401 gaggaagaat tgctagatac tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat
    26461 ctagcagata aaacatttca gtttgatatg gatacagtaa agggtgttgt ttattttaat
    26521 gatattaccc aaaaccagtt gttaaaactc catcagacga acagtcagcc ggtgattggt
    26581 attttatcta ttgataacta tgcagatgcc attgataaat tggatgacaa agagatttct
    26641 tatttgaact ctttcgtcac tacactagtt tctgactgga tgaatgaata ccatgtcttt
    26701 ttcaaaagaa tcaacgcaga acggtttttc ttcgtagcac agatgatcga tctgaaaaag
    26761 atgatggata aaaaatttga tttgctggat cgctttcgag cagaagctga aaaacagagc
    26821 tcgcctatca cccttagtat ggggattgct tatggctcaa gaaccctcga tgaaatcggt
    26881 ggagaagctc agaataattt agatgttgct ttagtccgtg gaggcgacca agttgtcgtg
    26941 aaagaggtaa aagaaggagc aaaacctgtt tattatgggg ggaaatctgc taagaatgtc
    27001 aaacgcacgc gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa
    27061 acaggcgatg tgtttatcat ggggcacaag tttccggata tggatgcttt aggctctgct
    27121 tttggtgtca gttgtctggc acgcttcaac cagaaaaaag cttacatcgt tatcaacgag
    27181 aacgagatca ttccagatgt agaacggtgt cttgaagaaa tccataagca tcctgaatta
    27241 gaaatgcaac taatctctcc agaacgtgcc atagagctga aaaaagaaga ggacctcctc
    27301 gtgatggtgg attaccataa accttctcta tcgatctctc agggcctata tgagcaattt
    27361 gataagattg tgatcattga tcaccatcgt cgtggggaag agtttccaag taaacctctg
    27421 ctgacttata tcgaatcttc agcttcttca gcttcagagc tagtttctga gctgatccaa
    27481 taccaaagca gcaaaacaaa tcgcttagat cgttttgagg ccacattatt gcttgcaggg
    27541 atggttgtag atacaaatag tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc
    27601 agctatttaa ggtcttgtgg agcagacgca tccttagtac aatatttatt gagctcggat
    27661 ttagcgacat acttggagat cagcaatttg attgctggaa gcgactatgt gacttctgat
    27721 attgttgtgg cggcagggac agacgaaaaa gagtataata gtgttatagc agcaaaaaca
    27781 gcagatacat tgctttcgat gatcaatatc aatgcagctt tcgtcattac aaagcggaca
    27841 gacggcttga ttgggatcag tgccagaagt accggcacga tcaacgtcca aatcatcatg
    27901 gaatccttag gtggcggagg acacttcaca aacgcagcta ctcaactgga aaatacgaca
    27961 gttgaggaaa caaaacagaa gctatttgct gcaatcaaag aaaatatgaa tcaaatatat
    28021 gataaggagt aatccaaatg aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa
    28081 aaggggacat caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc aaaaataact
    28141 tggcaaaaga agcaacgaaa agcagtatca gtcagttagc aggacaaaag aaagcacaag
    28201 aaaaacacga agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag
    28261 aagatactgt agtcgaattg aaggcgaaag caggggaaga cagccgtttg tttggttcga
    28321 tcccttcaaa acaaattgct gaagggttaa acaaacaata caaaatcaaa ttagataaac
    28381 gaaaaatcga attagaaaat ccgatccgtt cactaggcta cacaaacgta cctgttaagc
    28441 ttcatcatga ggtgacggct acgatcaaag ttcatgtcgt acaagaataa caaatcttga
    28501 tagcgatgtt cagctggtaa gtataaccag aaggaaaaaa gacaaaccga aatttatctg
    28561 agaaaacagc tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt
    28621 tcttttttgg gaacagcaaa atagcataaa gaaaacgagt ataataaaag agaactattt
    28681 ttttattaca ctttcggtac gaatgatgac ttttctctca ttttcttgta aaatgaagca
    28741 attgaagaca aggagcgata cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa
    28801 atattgaagc tgaacaagcg gttttaggct ctgtttttct tgacgcagaa acgatcattg
    28861 atgccatgga gtacatagag ccaaaagact tttatcgtcg tggccatcaa attatttttg
    28921 agacaatgat cgagttaaat gatcgtaatg aagcgattga cgtggttact gtcaaagacc
    28981 ggctggaaca agccaatttg ttagaagatg ccggtggttt aagttatctc tcagacttgg
    29041 cattagcagt tccgacagca gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat
    29101 cattgctgcg tacattgatc cagacagcaa caggaatcgt tacgaaagga ttcgagcaag
    29161 gagaagaagt tcaaacaatt ttagatgatg ccgaacgcag tatcttggag gtttcggaaa
    29221 aaagaaatcg aagcggcttt ttatctattg ctgaagtact gaactccacg attgaaaata
    29281 tcgatcgact agctcaaaac aatgaagaaa tcacaggtct accaactggc tatcaaggtc
    29341 tggataaaat gacagcaggt ttccagcccg aagaactaat cattcttgct gcacgtccag
    29401 cggtaggtaa aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc
    29461 gttctgttgc gatatttagt ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt
    29521 gtgcagaagg ttcgattgag gccagccatt tacgtacagg tcagctttcg gaagaagaat
    29581 ggtcgaatct gatcgttgcg atggggagtc tttctcgagc aaatattttt atcgatgata
    29641 caccagggat caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag
    29701 gcaatctagg cttgatattg atcgattatt tgcagttgat tgaaggtaac ggtaaagaga
    29761 accgtcaaca agaagtatca gatatctctc gccaactgaa aaaactggcg aaagagctga
    29821 aagtacctgt cattgctctt tcacagttat ctcgtggagt agaacagcga caagacaaac
    29881 gaccagtgct aagtgatatt cgtgaatccg gatcaattga acaagatgcg gacatcgtag
    29941 ccttcttgta tcgagatgat tattatgaaa gagaaggcgg agatgacgat gatggaaacg
    30001 agccaaaaaa tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag
    30061 gaacagttga actactgttt atcaaagaat acaataagtt ttcgtcgctt tcgccaagag
    30121 aagaatttta aagaagaggt tgtgagcctt gagcaatccc gagagctctc gtaagaagat
    30181 gcaaggagta accgcaggaa cgactagagt acttaattta aacagatctc caaagggatt
    30241 gtgacaaaag tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgaccc
    30301 ttggtattag ctcagcaaac gtcaaaatat gaggaactat tttgacgttt gtttctctaa
    30361 tactcaggtc aaaacgctga tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa
    30421 ctcctcggtc acaagtcaca atatcatcaa acatgagaag cagttttttg aaattgctcc
    30481 ttgtgactca gagttaaacg gcaggctcac aacctctttc ttaaacggtg ttcaaaagct
    30541 tgcatctgcg gtaatacgcc caatcaaacg aaaaatatga gaagctattt ttgtttggct
    30601 gttcttatta cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccaaaa
    30661 gtagcttctt cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca
    30721 ttccttctta tttcttgaag ctgaccactt ctgttacaac ctctttttta ataaccattc
    30781 taaaaagata aagttcgttt tttaagcgtt tttataaaat aaaaataaaa atcattcgat
    30841 tttatttgaa atatgtatct attattgata caatggttca ggattaaata atatagaacg
    30901 aggtgttcac atgtcatctg tagtagtagt aggtacgcaa tggggcgatg aagggaaagg
    30961 aaaaatcaca gactttctca gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga
    31021 caatgcaggg cacacaatta agtttaacgg tgtgacttat aaactgcatt tgattccttc
    31081 tgggattttt tataaagaca agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa
    31141 atcattggtt aaagaattag cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg
    31201 tatttctgac cgcgcgcacg taatcttgcc ttatcatatc aaattggacc aattacaaga
    31261 agatgctaag ggcgaaaata agattggtac aacgattaaa ggaatcggac ctgcctatat
    31321 ggataaagca gctcgtgtag gtatccgtat tgcagactta ttagataaag aaatttttga
    31381 agaacgtctt cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc
    31441 agaaccactt gattttgacg atatttttga agaatattat gaatacggac aacaaatcaa
    31501 acaatacgtc acagatactt ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt
    31561 gttgtttgaa ggcgcacaag gtgtaatgct ggatatcgac cagggaactt atccttttgt
    31621 tacttcttct aacccagtag ccggtggggt aacaatcgga agcggtgtag ggccttcaaa
    31681 aatcaataaa gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt
    31741 tccaactgaa ttatttgatg aaacaggaga acaaatccgc gaagtaggac gagaatacgg
    31801 tacgacaact ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc
    31861 taaacgagtt tcaggtatta ccaacctatc tttaaactcg atcgatgtat taagtggatt
    31921 ggaaacagta aaaatttgta cggcttatga attagatggc gaactaatct accactatcc
    31981 agcaagcttg aaagagttga accgctgcaa accaatctat gaagaattgc caggctggtc
    32041 agaagacatt acaggatgca aaacacttgc agaactgcca gaaaacgcac gtaactatgt
    32101 acgtcgtatc tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg gacctgatag
    32161 aacacaaaca aacatcttag aaagcgtctg ggcgcaaatc taaggataaa aacgacgact
    32221 gaatcaggac gttcttacca gagcgagtcg aagtgtttat ctatacgata ataataactt
    32281 gaacactgtt tattcagaat aagggactgt gacaaaaacc gtgtcacagt ccctttttcc
    32341 gaa
//
LOCUS       DACCKU010000024        31701 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.024, whole genome shotgun sequence.
ACCESSION   DACCKU010000024 DACCKU010000000
VERSION     DACCKU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31701
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.024"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(717..3866)
                     /locus_tag="IVN13_001052"
     CDS             complement(717..3866)
                     /locus_tag="IVN13_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP7011554.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3879..5078)
                     /locus_tag="IVN13_001053"
     CDS             complement(3879..5078)
                     /locus_tag="IVN13_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7011555.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5068..6663)
                     /locus_tag="IVN13_001054"
     CDS             complement(5068..6663)
                     /locus_tag="IVN13_001054"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP7011556.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7247..7954)
                     /locus_tag="IVN13_001055"
     CDS             complement(7247..7954)
                     /locus_tag="IVN13_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011557.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8019..8855)
                     /locus_tag="IVN13_001056"
     CDS             complement(8019..8855)
                     /locus_tag="IVN13_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7011558.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8873..10282)
                     /locus_tag="IVN13_001057"
     CDS             complement(8873..10282)
                     /locus_tag="IVN13_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7011559.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10296..12119)
                     /locus_tag="IVN13_001058"
     CDS             complement(10296..12119)
                     /locus_tag="IVN13_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7011560.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12962..13288)
                     /locus_tag="IVN13_001059"
     CDS             complement(12962..13288)
                     /locus_tag="IVN13_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011561.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13334..13696)
                     /locus_tag="IVN13_001060"
     CDS             complement(13334..13696)
                     /locus_tag="IVN13_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP7011562.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13733..13948)
                     /locus_tag="IVN13_001061"
     CDS             complement(13733..13948)
                     /locus_tag="IVN13_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7011563.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14075..14410
                     /locus_tag="IVN13_001062"
     CDS             14075..14410
                     /locus_tag="IVN13_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7011564.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14486..16483)
                     /locus_tag="IVN13_001063"
     CDS             complement(14486..16483)
                     /locus_tag="IVN13_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011565.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16581..16856)
                     /locus_tag="IVN13_001064"
     CDS             complement(16581..16856)
                     /locus_tag="IVN13_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011566.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17088..17822
                     /locus_tag="IVN13_001065"
     CDS             17088..17822
                     /locus_tag="IVN13_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7011567.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17861..19003
                     /locus_tag="IVN13_001066"
     CDS             17861..19003
                     /locus_tag="IVN13_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7011568.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19098..20489)
                     /gene="sufB"
                     /locus_tag="IVN13_001067"
     CDS             complement(19098..20489)
                     /gene="sufB"
                     /locus_tag="IVN13_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP7011569.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20494..20964)
                     /locus_tag="IVN13_001068"
     CDS             complement(20494..20964)
                     /locus_tag="IVN13_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP7011570.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20951..22186)
                     /locus_tag="IVN13_001069"
     CDS             complement(20951..22186)
                     /locus_tag="IVN13_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7011571.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22183..23469)
                     /gene="sufD"
                     /locus_tag="IVN13_001070"
     CDS             complement(22183..23469)
                     /gene="sufD"
                     /locus_tag="IVN13_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP7011572.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23482..24255)
                     /gene="sufC"
                     /locus_tag="IVN13_001071"
     CDS             complement(23482..24255)
                     /gene="sufC"
                     /locus_tag="IVN13_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP7011573.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24452..25300)
                     /locus_tag="IVN13_001072"
     CDS             complement(24452..25300)
                     /locus_tag="IVN13_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7011574.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25324..26010)
                     /locus_tag="IVN13_001073"
     CDS             complement(25324..26010)
                     /locus_tag="IVN13_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011575.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(26003..27037)
                     /locus_tag="IVN13_001074"
     CDS             complement(26003..27037)
                     /locus_tag="IVN13_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7011576.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27432..27770)
                     /locus_tag="IVN13_001075"
     CDS             complement(27432..27770)
                     /locus_tag="IVN13_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP7011577.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27772..28128)
                     /locus_tag="IVN13_001076"
     CDS             complement(27772..28128)
                     /locus_tag="IVN13_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP7011578.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28134..29321)
                     /locus_tag="IVN13_001077"
     CDS             complement(28134..29321)
                     /locus_tag="IVN13_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7011579.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29512..30357)
                     /locus_tag="IVN13_001078"
     CDS             complement(29512..30357)
                     /locus_tag="IVN13_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7011580.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30591..31556
                     /locus_tag="IVN13_001079"
     CDS             30591..31556
                     /locus_tag="IVN13_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP7011581.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         9183 a         6632 c         5333 g        10553 t
ORIGIN      
        1 gactaataca ggtagtcctc ttttttgtta ttttgatatt ctaaatcatt ttttattgga
       61 ttattccctt gttcgatata gacccaaatt gatctatcaa taattatcaa actgagtcat
      121 atagagacaa aaatttcaaa aatgacctgt taaatggtca tcgaatatca tttaaaatca
      181 atacgacgga gtcagacacg gagtcaaaac aagcccgctg agagtaaaaa atatatacag
      241 tcccataggt aaagtgccac aggactgtat atttttttat ttgagattac ctgaaatgat
      301 tccatttact ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc
      361 tcaataaact attttgggtt acatggtatc tgaaacagct aaaaccttgt cattacagca
      421 aaaaatcact ttatctccgt ttcatttcag atacatttca cttaagcgga ttccaaaaaa
      481 acaaaagaaa aaggaacccc tagttagcct atgcttttta ttggttcctc tcaaaacacg
      541 gtcatataac aacggggtgg ggtaaatgaa tgaaaaaggt ggaggaaaca ttattcaata
      601 gctgtcttaa atacttcgtt ataaaatgtg aacaaataaa agacatctat ccctattttc
      661 tacacttcca ctttactacc atctttgaat cgtacaattt tcttaactct tctcgtttac
      721 ctcaattcat tttcaagtgg aatcagctca tcatctaaca ttttacgcca tgctcgcttc
      781 atttcttgtg ggtatttaaa tggcttcgct tctggatggt tggctttata tccttcgtaa
      841 tccttactgt tgataattgc tttcatatta ggaattggat cttcacctgg catatattgc
      901 agattagaag catgtaattc tttctcagaa acaaaccact cattggaaaa gacatgaatc
      961 aaatcatcac tcgcttgtgt aaaatagcga cgtttaatga caaagatatc ttcattacta
     1021 tctaaagtcc ccacatcaaa cgcatttaaa atttgtcggt aaacttcttt gactccttct
     1081 gttttattca atttagccaa agctttttca atttcttctc gtgcatctga actattcgcc
     1141 gcataagttc ctagtaattg atcaatataa gtagaatcaa tatcatacaa cttcgtggta
     1201 ttttcactat aaaattcgat cgtggaaaaa tcttctccct ctttttctgg ttcttcctct
     1261 attaatgaac ccttcacggt ctcgtacacg ccaatttgtt cttccaaaag ttgaatttga
     1321 ttgttcaagg ttgaagattt caccatgtca tcattgtaat cgtcataagt cactaacgat
     1381 tcataagcac gattcaattc ttggaatgct ttgacgtact cgatacggat ttcaagtggc
     1441 gtatgttcat caatggattc catggatgaa acctctttta acttaccata tgcttgcttg
     1501 aatctttttt tggattcctg ataacttgga taaatcaaac ccgattcttc ttttgcttct
     1561 gaatacagtt ttgtcgcatc ctcgacattc ttctccattg tggctggctt tcggaaagta
     1621 acaattagtc cttttgtttt ttctggatac gtacggttcg tacgtgaaaa agcttggatc
     1681 aatcctgcat actctagatt acgatccaca aatagggttt gaatcgtagg tgcgtcaaaa
     1741 cctgtcaata gacgatccac tacgatcact aaatcaattt gtttcccaaa ttgtttgaat
     1801 tccgctcttt ttcgtgccaa acgattattg atgtctccgt tataacgctc aatgtctgct
     1861 aatgaccagg aagtatcata atactgatta tattcctcaa taattttttg catttcttct
     1921 tgttgggcac ttgaatctcg gctattttct tctaatgaat aggtaatagc gattcgtgga
     1981 aaatcaggat cctccatcgt tcgtccttca cgaatgagct gattgggaaa ctcttttgtt
     2041 aaccaatcag gctctttcgt catctctttg atggcccgat aatacttttt ggccatgtca
     2101 atggaactcg tggttaagat tgcagatttc gttggcctgc catttcgaaa atcaaatttt
     2161 gtataggcat tatctggacg gaacatttta tgaatgactt tttggatatg ttcatctgct
     2221 tcaaaaacag ctgggtccaa ataactttct tttttgacag gttccatttg atcaatcata
     2281 cgattgatct gttctgatga atactctgca tatgtttcta cttcttttaa tttccgatag
     2341 attttattct ctagtgaagt cggctccacg gtgttttcat gctctacttg aaatcctaag
     2401 acagagccat cttctagcgc attcttaatg gtataggtat gcaagacctc tccatactga
     2461 tcatgggttg tacgtgccaa ttgccctttg gcttgtttcc gattctcagg aaaaatcggt
     2521 gtacctgtaa agccaaacca agtggatttc ggaaacattt ttttgatttc ttccatattt
     2581 tcagcactta acgctcgatg acactcatct actacaaaca cgatatgttg gcccattaat
     2641 ttttggaagc gattggttcc ttttttctct tcttgtttct ttgcatattt caacgccgca
     2701 tctagttttt gacgggttgt aatgattaca acatttgcat tggcatctgc caacagggtt
     2761 tcacttaatt ctttggcact tcccgttcca acgatcaagc tattcgcttt ggcgttcccg
     2821 gaagaaatcc cagtattaaa ttccgaagca aattttgtaa actctgtggt ggtttgatta
     2881 tctaaatctt tgcggtccac taacatgatc gttcgatcga tccccgattt tcgtgctaaa
     2941 agcttagtag atacaaaact agtcaacgtc tttcctgaac cagtcgcatg ccacacatac
     3001 cctgattgat gtttatttgc ggcaataaac aaggcttgaa tcgcatgtac ttgataagga
     3061 tgtaacacca tgagggtttt attatcttga tcttcactga cgattgtata atccgcaatc
     3121 aaacgatgag catccgggat atttaacact tgtttacaaa actcatataa gttttctact
     3181 ttttcattgt ctttcgttcg ccaactaaat aaaaacttag ggtgcaagtc tttcggtaag
     3241 gcatttgcaa aataccgagt ggtttgttca ttggaaacca caaacaactg taaggtcgaa
     3301 aagatattgt tccgaaacat accttcttct gcatattttt tgatttggtt aaatgcctga
     3361 taaacaccat cttttgcagt tacttgtttc agctcgattt ggacaatcgg taatccattg
     3421 attaataagg tgacgtcaaa acgtcgatcc cgtgcttcta tgttacttcc acgttttgca
     3481 atttgatgga ccacttcata agtagagatt cctccaccaa tgtcttgatt ggaataaagg
     3541 ataagtgaga cagaacccaa ttgcgaatct tcccgttcaa tcgtgatccg agccatccca
     3601 ttttcacctt ttaaccattt cgcagcatca aatggggttt tcgttcgcag cagtaattcc
     3661 gttttgatcg tttcaaattc tttatcggtc aaaggaaaat cattcagttc tgcttgatta
     3721 tttgagataa ttttttggcg taaattgacc caaaggtctt cttctgattt tagatccgga
     3781 cgatacgtcc actgattatg cccttctcca agtacacgaa tcaactgttc ttcgacttct
     3841 gcttcctcac ggtgactaat tttcattctt cttccctcct agacaaacat cttttgtaag
     3901 aagctttttt tcgtttcttt tagtaaatct aacttacgtt gatgaagagc gatcgtatca
     3961 tctagctgtt tgaagaaagt accgattttt tgttgttcat cctttacagg aaccttaaca
     4021 ctgatattct caatatcaga actgttataa gaaggcaatg cccctacttg ggagtatctt
     4081 ggcaaattta tagtttcaaa caatatttta ccaaaataga tatcccaagt attattaaat
     4141 aaataagcca tggtattgtt atctattaag aaagggcttg tgaccaaacg ttttctatta
     4201 agcattattg ctgcgccaac tttagcaaat ataatcgctg gcacatcttt tattggcttc
     4261 cattttttac gctcaatttg ctcattagat acataatgat ttgcatttct catttcatac
     4321 tcattaccaa tgttattcat gtcggaaact ttaaaaaatg gagtaccttc agaaccacct
     4381 tgctcggcat caggaaatcc aattcccgat tgagttttac ctaattctcc caacttacgc
     4441 tcttcccaat cttccgtaaa accaggaaac cggatttctg gcacttttgc tccgttttca
     4501 gggaacattt tttgtaagaa tccttttttc gtttccttca gcaaatctaa cttacgttga
     4561 tgaagagcga tagtgtcgtc gagttttttg aagaatatgc cgattttttg ttgttctttt
     4621 aaatcattgg gaatatagaa tactgtatct gaaacttttt tccagtctga acgaggcatt
     4681 tttgttccag atgtatcatt tgcagccttt tgatatctat cagcttgaat aagagaataa
     4741 ataaagtccg agtcgctctt cttagctcta aaaacccaga aatccccaat cgctacgcct
     4801 gtaaaagttg ccttgagcca attttttaaa tatggacgta actttccata tagaatatca
     4861 tttggtaaaa attcaatccc ttttctatta tcaaatttct ggctcacatc tttattcagt
     4921 ctaccttctc ctgccacgat atcttcaaat tcaacttttg gaagtctact ggagtttgta
     4981 cttttattaa gtctaacaac taaatcaatt aacttacgct cttcccaatc ttccgtaaat
     5041 cctgggaaac ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     5101 ttgtaaccac atttcgtttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     5161 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     5221 atcaattggt tcttcttctt cgaaagtatc tacatagcgt gggatattta agttatagtc
     5281 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     5341 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat agtttatttt gatttttccc
     5401 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     5461 caacacaatc accgtcgttg gaatcgatgt cccaaagaac aagttcgcag gcataccgat
     5521 cacagcataa atactgccat cttctagtaa tttttgacgg atgacccctt ctgcagcgcc
     5581 acggaataaa actccgtgtg gtaagacgat ggccatggta cctgtttcct tcaaatgata
     5641 gaacccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccataacg
     5701 attaaaccga gaatcatcta agaaagtcgt atctgctgac caattagccg aataaggtgg
     5761 gttcattact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5821 atccccattg cggagattca tttcttcggc gtcgacacca tgtaaaatca agttcatttt
     5881 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctgggtg
     5941 tgttaagtag ttacgtacgt ttaacatcaa tgaacctgaa cccatcgttg gatcaaaaac
     6001 actaaagaat cgtcgttcct tttgatcgag cgttacgatt tgcgccatca tatcagaaac
     6061 catatgaggt gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     6121 cagaaactca tacgcatccc cgatcacgtc cccatcgtgt tctaaaacat ctacatcatt
     6181 caatttcttg atgacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     6241 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     6301 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     6361 taagacgttg aaaagatact ctggtgcaat tgcatatccc aaaatatcaa cgattgtggc
     6421 aatcagatct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     6481 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     6541 tcgatcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     6601 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     6661 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     6721 ccccctttta taaaaagcaa aaaaaaaaga aagaaactta ttcttgctaa taaactacgc
     6781 gaaaggagtc taacgttgtt tgatataact tagactgatc gttaacttat tttttatatt
     6841 tctacatagt tatctttaga accatttcag atacatatga aacaattaca tatgattttt
     6901 tcataaaaaa acagtactgt ctagtagtat aaaatataca aatttcttaa aaattttttc
     6961 actaaaatca ctacctgaaa cagtctaaaa cacttatata tcaacgttta atgaaactat
     7021 ctccgtttca tttcagatac aaataaatat agtcgggctc caaatccgaa aaaaaatacc
     7081 cccagtagct tatcctaagc tactggagat gtataagtac ggtcatataa caacggggta
     7141 gggtaaataa atgaaaaaga tggaagaatc attattcaat aactttttta aatacttcgt
     7201 tgtaatcagt tacctgtccg ttcttaaata ccacagaata atctttttac ccattttcaa
     7261 gtaaccgaag tggaatgtct ttaccaactt gctctgtcac tgcattactg atacctgtta
     7321 gattagctaa taaatctgat aaagattgag catctgtaga aatctctgta tcagtattta
     7381 tcgtgattcc tttatcttca tttacaagct tccaagtagg tggtatagct gtgtatgttt
     7441 cgatgaatga atcaattctt tcatctatcg tcttcccagt atctacttct gtctcattac
     7501 tcttagatga atcgattctt tcatccatcg tctgctccgt aactgcttct gtctcattat
     7561 tctcaccgga aactctcttc acacccaaac ttatatattg tccatcttta gtgtgcatag
     7621 ttaaaattcc cacgctttta ctaataaatt cactataacc agttctttca atagagactt
     7681 caatgtattt cacactatca tactcaaaag aaaaatagac atgtaaaagc attttgttgt
     7741 ttacatctac ataataatcc tttgactcta cttgagacct tcctatacgg aatacagctt
     7801 tactgtctga tatctctata ccagcatcct tattattaat ctgccaatct ccaagtagct
     7861 cttctggcaa gctagtagtt tcttgtgaac actttaccac ttcactggta gaggtcgaaa
     7921 cattcacctc cgcactgtta gaagaatttt ccaaggaatc aggagcctta gcacaaccaa
     7981 caagtcttta tatattgtgc agccgatatt ttcttcattc agtacaccct tgaaatatgc
     8041 agtgttagat aaagcagttc gtcctgattt gctgaggcac ccatctcttc ttgaacgtac
     8101 tccttgattt tcaaggccgc ccgatactct ttcggaaaac gctgcttcac tgaagccagc
     8161 aaaaaatcat ccttctccgg caactcttcc ttcttaatca ttcgggcaat aaaaaactgt
     8221 aaatgcgtca caaatcttaa gtaaagcatc gaatctttat caaccttttt ttcgggaaac
     8281 agatatccta gcatagccaa gatatccttt agtgctctta ccgagagcat ggtttcttcc
     8341 atatttgctt gttcactttg ggcatttaca atgtgaaacg caatgtttcc agcctcctct
     8401 tctggcaact gaacattcat ttttttgttg atcaattgga tagcatagca gccgatctca
     8461 aattcttttg ggtaatactt ttgaatttca atcagcaggc gattttgaaa aacaatgcct
     8521 ttttgactgc gttcaatggc atgcttcaag tgatcaacca aggtaaaaaa tagactttta
     8581 ttcagcttaa agtcaaactt ggcgttggca taatcgataa ttcgcttgac gcaaataatt
     8641 agatcatccg gagcagcctc aaacagttta atgtaatcct tttttagagc atcagaaagt
     8701 gaatctaatt taaatacttt ttggattttg tcagccggaa ccaaatcgcc tttctttagt
     8761 tgaaatccaa tccctttccc catcaaaatt acttctttgc cttgcttatc taagcaaaga
     8821 attaaactgt tgtttaacgt tttaattact ctcattagat tcaccgttct ctctaagcta
     8881 agccatttct tgcgattact ttttgatacc aaaagaagct ttcttttttt attcgtttta
     8941 attctttaag attctcatcg gttcggtcaa catatataaa accataacgc ttattgaagc
     9001 cttgatgggt actaacaaca tcaatcgctg accaaggtaa ataaccaaaa acatctacac
     9061 catctgttaa agctgcttga atagccagaa tatgtttggc tagataatca attcgatcct
     9121 ggtcattaat tcgtctatct tcctcaacta tgtcgggtgc cccatagcca ttttcagtaa
     9181 ttaaaatagg taaatcatag cgatcgtaca atttcctcaa tgttaatcgt agtccagtag
     9241 ggtcaactac ccagccatat ggcgttttat ctacataagg attttcagct gcccggtaga
     9301 caccttcttc tcccaacatg atctgttgat cccccgctct agcggaaaca tctgatccgt
     9361 catttttgct ggcagcgatg gtggcagttg aataataatt aatcgcaata aaatcgggtt
     9421 taccggagga taaaattgcc tgatcctctg cctcaatttt aggatataat ccacgatcat
     9481 tcaaatagtt ttcaaacaaa taattgtatt ttccccgaac tgctacatct aagaaagacc
     9541 agcctctcaa tgtttcccaa ttatgggctg caattgcatc ttctggactg gaggtagctt
     9601 gatacatagc cgttaaattg attgctggac cgatttttcc tttaagattc aactgatgat
     9661 acaagtgaat tactctagct tgagctaata aaacatggtg attaatttga aagagttctt
     9721 tttttgaagg cagctctcca cttggtggca aaccaatcgc tccaggatga aggatcatcg
     9781 tgttttgctc attaattgtc agccaatagt tcaccttgtc accatactca gtaagcaata
     9841 cctgtacata gtccacaaaa taatcgatag ttgctcgatt taaccaaccg ccctgctcct
     9901 ctagaaaata aggtaaatcg aaatggtaca tggtgacaat cggttcgatt ccctgattca
     9961 ggcattcatc aattacttga tgataaaaag aaattccctt ttgattggtt ttattatttt
    10021 caatatccat aattcgcgtc caagcgatgg aaaagcgaaa agcttttaag cctaattctt
    10081 taaacagctg tatatcttct ttatagtgat ggtaaaaatc cgaggcaact tcaaaattag
    10141 ctaccccttc tgtatgctga tacttgtcaa taatagacgg ttgtttccca tcctcctgat
    10201 aggcaccctc tgcctggtag gcactaatgg aggcacccca taaaaacttc tctggaaatg
    10261 ctgaaagctg tttgtattgc acaaaaatcc tctccttaac taatttgcat caatggttcg
    10321 tgttccttta agcttccgct tgcttcacta attcctgcgt aattttcact gttggtcact
    10381 accacaatca ctgtatcatc gtagccagcg tttctaatgg cagcgaaatc ggcgttgatt
    10441 aataattgtc ctgctgacac ctgatctcct gcttttacaa atacttcaaa gggttcccct
    10501 tttaaattca cggtgtcgat ccccacatgg atcaataatt caataccttc tttagaacgt
    10561 agaccaatcg catgtttaga ttctaaaata gtaatgactt ctccctcaac aggtgaataa
    10621 atgctgtttt cttctggatg aatggctacg ccctttccca atagtccctt agaaaagaca
    10681 gcatcgttca cttcagaaat aggacacacg gttcctttta ccggattttc aacaatgact
    10741 gcttcggcta ttgccgtttg ctctgattca tcagaagtat cgtcaaatcc aattagataa
    10801 gcaatgataa aggttgcaac aaaggcgatt acacacgtaa taattgcgtg aacaatattc
    10861 attggatttt ctccaataaa ggctggtaag gctgctaagc caggggaaac aaaggcatat
    10921 ctgactaaac cgctcaagcc agcatacaaa ccagcaattg caccaccaat catggaagca
    10981 actaatggtt ttttcagctt caaggttact ccatagagtg aaggctcagt aattcccatt
    11041 aaagcagtga tcccagtgga cacagctagc tgcttcaact ctttgttttt agttttcgcg
    11101 gctacagcaa aggtagcacc tgattgagca atattagaag ccagcattcc tggcccattg
    11161 atcatttcag aacccgtact gctcaattgc atagtagcga ttggcgtcat tgcccaagct
    11221 gtacccgtaa ccaccaaaaa tggctgcaaa ccacccatca aaaatggaat taaccaagct
    11281 gctttgccgt taatccattc agcccccatt gctataagat cgttcagcaa cgttccaaac
    11341 ggtccaatga caaccaacgc taaaggcccc ataattaaaa ataccagcat cggcttagta
    11401 aagaatttaa taaacgaagg cgaaattcga tccgcaaagg gctctacata ggacatgatc
    11461 caaacagtaa agataatcgg taaaactgat cccgcataat ctgcagctaa taccggaatt
    11521 cctagaaaag aaacggcatc gccagtagaa aaaagctgtg aaatatttgg atgaagcaaa
    11581 atgccagcaa cagtaatcgc caaaattggg ttgacatcaa actttatggc tgccccatag
    11641 gctaatagta ctggcataaa ataaaaggct gcatcagcaa tagtattcaa aatgatgtaa
    11701 gtttgactac tgtctgatag tacaccagtc aaagtcaaga cggccaaaat ggcttttacc
    11761 atcccggcac ctgttatagc tggaatcatc ggtgtaaagg tggtagaaat gacactaatc
    11821 aatcttgaaa taatactttc ttgattgtct gaagaggggc cctttttaat tgaagttccc
    11881 acttcctttt gaatttcctt gaaggctgtt tggacttcat tgccaatcac cacttggtat
    11941 tgtccaccgc tatccaccac actaattaca ccaagtgttt tttctaaggc tttcgtatcg
    12001 gctttccttt tatctttgag ttcaaaacgc agtctggtgg cacaatgggt taagctgacc
    12061 acattttctt ttcccccaac ctgttgcaat acatttcttc ctacttctct gtaatccatt
    12121 attctctctc cttaaagcag taaaaaagac caagtcaaac cacgccccaa tcaacattga
    12181 agtgttagtt tgtcttggtc ttgcctgcat aatcagtaac atgccaataa cgttattttg
    12241 ctgtaatcca ataatagcac attatgatag cgcaatcaac agggaattta attttttatc
    12301 ttttcgtttg accgtatagg gtaatataac agttagatca catcaactag ggtatatctg
    12361 cgtatactta ctgctaaaat aagtatatct aatattaact ataccagtaa aaccattcct
    12421 acataaccag cgggaaattc ccaaaatcgt gaaagcatca aaaatgcata taacggatca
    12481 cacaacttca acaattctgc tcttgggaaa aattgatgaa tttggtgagt aaaggatgga
    12541 ataaacagca ttccaacaat ataggcaaca atagtgatac ttgttttttt ggtaaaaatc
    12601 agtaagcaat agccaatttc aatcattccg agatataaag gtatcaatag aatgatttgg
    12661 gctagtattc ttaaaaatta actagcaaat gtttaaactg atttatttct aatctacatg
    12721 tagtaagtat ttcaattcca ccaattatca ataaaaatac caaacatatc cataataatt
    12781 ttttcttttt catttctttt atattgttat aacagtgaat aacataaaaa ttcccgacaa
    12841 tcattgtaat atttttttgt cacctgtatt agtatttaat ttataggaaa ctataaaaac
    12901 tccctagtga atttacacct ttgggagttc ctgtttggac ggtatgcata atacatttaa
    12961 atcaatttaa cattaacacc tcagcatatg gtatctccac atatatacgg tctttctttg
    13021 gatagtactc aatatttatg ttgatagcac ttccattgtc taatatagct ttagcccggt
    13081 atgtgttatc tccattttca tgagtaatcg tcacatcatc acatgatcga tttaagttgt
    13141 actgttcttg taaaatctgt gttaccaatg gttttgatga cttctctaat ccagatgtac
    13201 ctgtcatttt tgaaaaaata atacctataa tagccaagac aacaatacct ataatgacaa
    13261 acttcttgtt catatttact ttattcaact ttttatctcc tatctccaaa ataatttgtt
    13321 cttatccctc ttatcagtac attgacatcc ctaaaagtac accaaagatt gctccagcta
    13381 cccctgctat catcaaaatc gtacccattt tctcatcttt ttctcgatat aagtagccca
    13441 taataacagc aacagcacca aatacaacag gaataaatgc taacgatact accatagaaa
    13501 tccaaccaat aatcaaatac agcttccctg tctcttgtcc atgtacgatt tgttccttca
    13561 tttcattaat tgtctgctca gtacttttct ttactgtgtc tgttccggtt gtctcatctg
    13621 tttcttgtag tgctccacaa aactggcaat acttactttc atctgttatt tctttgccac
    13681 attttctaca atacatgtct tctcctccta aaatcattca attgcttatt agttaactgt
    13741 ttactttatt aaatacatca gaaaacagct cattcattgg tacacttaat atctcagaaa
    13801 gcatcttcat ttgatagggt ttaggaatcg ttctccctgt ttgccacgaa cttacacatt
    13861 gctgtgtaac attcattttt tttgctaact ccgtttgaga aagtcctttt tcttttctgt
    13921 atcggctgag attacttact tttttcatga ttctcccctc ctgttttatt atatacaaca
    13981 atatgatgta tttcaactca taaaatataa aaaacttcaa ataccattac aaatacaaca
    14041 acttgttgta ctatactgag atgggggtgt attaatgaaa cagaaacaat atgcaaaggt
    14101 cattagagaa aagcgaaaag ctctcgggtg gacacaaaaa gaattagctg aaaaaatctt
    14161 ctctacacaa caggcagtag caagatggga aaattctgta acagagccta acctcgatag
    14221 cttaacagct ctttctagag ccctaggaac ccctgtaagc cattttctag acaatgttgt
    14281 cgtagattat gaggaagagt ttttggctct ctatcgttct cttagcactg aagatgcagt
    14341 tcgaacaatt gactatatga agctattaaa aaggcaagaa aatgaacgca atcagctatt
    14401 gaaagattga aaaataaata acccaccgaa ctttaacggt gggtgtattg agatgaaata
    14461 agattaagag ctatggataa cctgtctagt ttaatacaat tgggtctttt ctcttcctta
    14521 ctctccgtcc atcatctctt gttacacttg taaattgata cagaggaact atcccagctc
    14581 ctgataatat caattctttt attgtttcat ctatccctgc ccctttcgct aaatatatta
    14641 aaaaactact acttgctttc acattgtata gtttcttaat atccttagaa gctcgttcaa
    14701 gagtgaatcc ttgtttaaca aagtagcctc tgttttttat taaagcattt ttcacgctgt
    14761 tatccccaac attacgtagg cgttcacaaa cagtgttaat aagttctttc tctgtcatta
    14821 gatatgtccc ttgccacgat aagccctgta aaacttcatg tgcttcatct aaaatcaatt
    14881 gtttttcctt gctgagttct gatcgggaat tttgaggaaa tactctttta gcctcttttt
    14941 cgttaaaaca tatcttaacc cattcataag aaaaatattg atatgtaaaa tgaatttttt
    15001 ccagaagtgg aaggatatct tcattcaatt tttgaatcac agaagttgca ttaaaaacgt
    15061 ctaatcgata gacattgctt ctcttatgat taaagtttcc taacgctttc tcttccttta
    15121 acctatttag gtattcgagc atcttcaaag gaacatcttc atcatctaat ttttttatca
    15181 atcctataaa agtcataagt gatagtaact tattacattt atctatgtca taagttttac
    15241 catagtaaga actcattttt ttagctaatt gttcccccga caaaatacaa gtcacacttt
    15301 ccttactatc atcaaaaaaa ttaacatcag aaagcaagac atcatagaca cgtcgcattt
    15361 cctctagata attccttatt gtcctgaaaa gtttgtcttt cattgattca agagtgtcaa
    15421 aaagtacttt tgattcctct ttcaattttc gaaaatgtgt aggatacaag tctaggttct
    15481 ctaatagcca attaaatgtt tctacccgac tgtctttctt taaaattagc atcacaactc
    15541 caataaaatt ttcactgata tcacaattac tggaatgaca ataaaaaatg gaatgcccag
    15601 ttttactggt aaaaatacta gcagagggat tatgatcgtt gtgaaacaga catcttaagt
    15661 ttttacttga cttaagtaat ttattcatat caatggttaa caatctttca taaatctcat
    15721 taaaagacat gtcggaggaa tcaaacaggc agtctttaaa ccactgtttc atttcttctt
    15781 tgtcgtcatt ttttatcaac ttaacaaaat cggtttcatt gactagttca tactctctcc
    15841 ctgaatcact ttgacaattt ttagaacatg tcatggtaaa atcaatcgga agctcagtaa
    15901 tatctaacat gttgtcttca ttaattatta tcactgcatc tgacttatat cctccaaaaa
    15961 acattctatt cgaagcattt acattgtcat cacacccggg gatgttcttt ttcaagtatt
    16021 cgtaaacagc aaccatttta ccatagtcag taattgcttc ttttaataga aagacaatct
    16081 tgtatttttc tagattttca gagtaccgaa tacttttttg tatcgcaaac gcagaattat
    16141 gaactaaatt gatagataaa accttttcta tgcttaagta tctactatca tctttactca
    16201 aatctactac cttccccttc tcatctttaa cagtgttatc gacatccaga ataattactc
    16261 tggtttccac aaaactctcc ttgtcattgc ctttattctt tgtcttgctt ggaataaatg
    16321 attgtgatga atttagtatc tctacaaact catctcttgt aacttctcta tagttatcta
    16381 aacacctatc tcttacatca gttttttctt tttttgtaac tggcttctct aataattttt
    16441 cttgatctac ataaattgga atattaatca ttgatgtttc catttgtgtt tcctccttat
    16501 aaaattctgt taaagactaa aacatacaga taataaaaac aaactcttta ttactatatg
    16561 ttttagtctt atttgtttac tcaaccttta taccgttcca tgaaactatc aatatcagat
    16621 tttttaaatc taatgaccgt atcaattttt ataacaggta acccgtattg agaaacatat
    16681 tttctaatcg ttcctactga tacacctaga tattcacttg cctgctgata gtccatatac
    16741 tctagagtga ctccagaaat aatgtcttct agcataggct ttagcactgt aattatctct
    16801 ttcacttgtt gttcaacttg tatagaaaca atgctatcaa tgatttgtag agacatattt
    16861 ccccctcctt tcgtgattgc ttgaacaaat tttctaagaa ttatcttcgt attttttgct
    16921 caaaataaaa gtattttgtt attaaccgat taatcaaaca aaaaatgatt accagttcat
    16981 cgacatacac ataatacctc gataaaaaag gggtgtcaat agttttgtaa aatttattta
    17041 caacacccct tttttgaggt atactaattt taggaggtgt ggtcaatatg ataaaacgtc
    17101 caccaataaa ttatttagaa cgaaaaaaga tactcggaac aaaaataaaa gctataagaa
    17161 agagtaaaaa attaacacaa ccagcatttg gtcttatgat taacaacggt caattaattg
    17221 acaaaaagac tatctacgaa tgggagaagg gaacctattt accaatacca gagcgattat
    17281 cccgaattgc tgatttggga aatatgagta tagaggagtt agtttgtggc aatgtagaag
    17341 aatatatttt gggaattatt ctttatagag atagcattgt actagatggt ataacatttc
    17401 cagataaaaa cctgttccag catcttcgtc aacagttccc accagtacac tctaatttag
    17461 atacatggct ggatagatat tccaaattag aaccagagat gcaagaattt attgctaaca
    17521 agacttgtaa taaagttaaa aatgaaaaaa ttagtctttt caatatctta aaaatagaag
    17581 aattattcat caatgctatt gtagaagaat ttgacaataa cattcttttc ctcactagca
    17641 gtatagaaga actactagaa agaatggtag atgaatggct acctattcaa ttgaaagaca
    17701 tgtcttatcc cgaagaagct gtgagagaaa ttactgataa tataaacaag ctagaacaaa
    17761 cgatttcatc aattgggaaa aaatacacta agaaaaaaat gaagggaggt gataccatct
    17821 agtggtaagt actccttgta aactaagagg agatgatttc atggcaaaaa tatcaaagta
    17881 caaaaagaaa aacggtcaag tggcgtggat gtataaagga cacattgcca ccgatccacg
    17941 aactggtgaa aaaataaata ctacgagaag aggttttagt agcaaacaag aagctcaaaa
    18001 agattatgac gagtacagac atcaaatatt atacggtgtt aaaaagaaag cacctgatat
    18061 gacatttgaa gacctgtata atgaatggat aactcatcag cgtaccagtg ttaaagcttc
    18121 aacaatcgca atctcagtaa gatacgcaaa taatcaaata cttccagcat ttggtaagct
    18181 taaactatcg aatatctctg tcccatattg tcaaaaagtg gtagatgaat ggcacagtaa
    18241 atatgaaagt tacgattata tgagaaaaca gactgcacaa attttacgct acggggttgc
    18301 aatgcaatat attgacaata atcctatgga aaaaacactt cttccacgaa aaaaagagta
    18361 tgagaaaaat cgtaaatttt actcaaaaga ggagcttaat aatttactgg atgcttttaa
    18421 agattttggt aacatgaaac aatacgcctt cttcagatta ttggcataca caggtatgag
    18481 aaagagtgaa gtactcgctt tacagtggaa agacatagac acgtttaata aagaactaca
    18541 tgtcaataaa actcttgctg tagatgaatt tggtaaagtt ataattcaat ctccgaaaac
    18601 aagagcttct agacgagtta tctctttaga tactgagact ctttctatcc tcaacaactg
    18661 gaaattacag caaaaagaag agtatttaaa acttggatac aatactagct ctaaagagca
    18721 acatgttttt acaacagtta aaaacactct atacattcca aacactgtta atgattggtt
    18781 aagatatatt ttgaaaaaat ataacttgcc tcgtattaca cctcatgggt ttagacatac
    18841 acatgctagt ttattattag aagcaggaga atctgtcaag gtagttcaac aacgattagg
    18901 gcatgaaaat agtaaagtga cattagacat ctatgcacat attacaaata atgcacctaa
    18961 gaaaacaggt caagactttg ctgatatgat ggctcatcag taaaagagct ggtcaaaatc
    19021 tggtcatttc tagtatacag gcaaaacaaa aaagctagaa cccttatata tcaagggttc
    19081 tagctcctat aacaggttta cccaacactg ccttccatct cataactgat caatcgattg
    19141 agttcgactg catattccat tggaagttct ttggtgaatg gttcaacaaa gcccatgacg
    19201 atcatttcag ttgcttcaga ttcggtcagg cctcggctca tcagatagta gagttgttct
    19261 tctgagattt ttgagacttt ggcttcgtgt tctaaagaga cttggctgtt gtggatctca
    19321 ttgaatggga tcgtatcgga tttcgaccat tcgtccatga tgatcgtgtc acattcgata
    19381 tgagagatcg aacccgcact gtttttggca aaggttactt gtcctcgata gtttacttct
    19441 ccgccgtctt ttgaaattga tttagagacg atagagctag atgtatttgg tgcattgtgg
    19501 atcatttttg cacccgtatc ttggatttgg tttgctcctg caaaggcgat ggagagcatg
    19561 gtgcctcgtg caccttttcc atctaagtaa acacttgggt atttcatcgt tgtttttgca
    19621 cctaggtttc cgtcgatcca ttcaacggtt gcgccttcgt atgcttttgc tcgtttggtt
    19681 actaagttgt acacattgtc tgaccagttt tggatcgttg tgtaacggca atacgcatct
    19741 tttcttgtaa agatttctac gatggcggca tgcaggctgt tacttgaata agtaggtgcc
    19801 gtacaacctt cgacatagtg gatgcttgct ccttcgtcaa caatgatcag cgtacgttcg
    19861 aattgccccg tgttttccgc attgatccgg aagtacgttt gtaatggaac atcgactttg
    19921 acgccttttg gcacgtaaat aaaggtccca cctgaccata cggctgaatt cagcgctgct
    19981 aatttgttgt cggttggcgg tacaagtttt gaaaagtact ctttgaataa ttcagggtat
    20041 tctttcaaag cagagtcagt gtcagtaaag acgattccta acttttcgaa ttcatctttc
    20101 atattatggt acacgacttc tgattcatat tgtgcggaag caccggctag ataagctcgt
    20161 tcagcttctg gtataccaat cttttcaaat gtatctttga ttttttctgg tacgtcttcc
    20221 cagtcacgag cgggtttgtc acttggtttt tggtaatatt tgatggcatc gaaatcgatg
    20281 tctgaaaggt cggggcccca tgtttgcatt ggcattttgt taaatgtttc tagagatttc
    20341 agacggaatt caagcatcca ttctggttcg cctttgattc ttgacatctc acggatcact
    20401 tcttctgtca agccttctcc tgtactgaaa accggttcga catcatcgtg aaacccgaat
    20461 ttatattctt ctaattctgg tacgcccatg ttctcactcc tcttccttgt ggtaatgatg
    20521 gaaagctgcg gtaccatcgt tttccattgc ttttcctagt gctttccagg caagtgtcgc
    20581 acatttgatc cgagcgggaa atttagctac accaccaagc atagcagcat cgcctaattg
    20641 gtcgggatct ttgacttctt ttccttggac aagctgtaga aagtctacag ttaaatgttc
    20701 tgcttcttct attgattttc cgatgacggc gtctgtcatc atcgaggcac ttgccgtact
    20761 gatggagcag ccactgccgt caaaggcgat atttttgatg attccgtctt ttacatcgac
    20821 ttgcaattcg atcacatctc cacatgtggg gttgttcaat tcgatcttgc ttgtcgagtc
    20881 agtcaagctt ccacgatgat gcggatggga agaatgatcc aagatcactt gacggtataa
    20941 attgtctaat ttagatagtc ccatgttgga aaaactcctt tgttgccatg attgcttcaa
    21001 ctaaacggtc ggcgtcttgc gttgtgttgt agaaataaaa gctcgcgcga gcagtcgctg
    21061 atacttcaag gtacttcaat agcggttgcg cacaatgatg acctgctctg acggctaccc
    21121 cttccatgtc taaagctgtc gcgacatcat ggggatgtaa cccatccaaa ttgaatgcaa
    21181 cgactcctgt ccgaaaattc gggtcttgtg gaccataaac agtcaatcca tcaattgctt
    21241 gtaattttgg caacacataa gcggctagtt cttcttcata tcgatggata ttgtccatac
    21301 cgagtttttg caaataatcg attgcatggc ctaatgcgat agcgccggcg atatttggtg
    21361 tccctgcttc gaatttccaa ggtagttctt tccatgtact gtcgtataat tcgacaaaat
    21421 cgatcatttc gccaccaaat tcaacaggtt ccatttcttc taaccatttt cgtttaccat
    21481 ataaaacacc aatacctgtt ggtccgcaca ttttgtgtcc gctgaaagca aagaaatcac
    21541 agtctagtgc ttggacatca atggccatgt gcggggcaga ttgagcacca tctactacga
    21601 tgaccccgcc gttttcatgg acaagttcag ctaattcttg aacaggattg aagacaccta
    21661 agacattgga tacatgagca atggagacga ttttggtttt gtccgtaatc tttttcgctg
    21721 catcctccat atccaggaag ccttcttctg ttagttcgat atatttcaag acagcacctg
    21781 ttcgttttgc caactgctgc cacgggataa cattagaatg atgttccatg taagagagca
    21841 ctatctcgtc tcctgctttg atatagcggt cgccgaaggt tctggctacc caattcaagc
    21901 ttgtcgtcgt accacgagta aacagcactt ctgctgtttc ttttgcatgg atgaacgccc
    21961 gtactttttc tcttgcttgt tcataatcat gggtcgcgcg ttctgctaac gtatggacac
    22021 cgcgatgaac attggcgtta tcgtgttcgt aatagtacgc taatgcctgg agtacttgtt
    22081 ttggcttttg ggtcgtcgct gcattatcca gataaactaa aggttcatca ttgacgtctt
    22141 gaaataagat aggaaaatca ttgcgtagct ttttagcatc gatcatccat tcaacttccc
    22201 ttcgattact tcgactaatt ctttccgaac ttgttctaca gggattgctg ttaagacaga
    22261 accgaggaat cctcgaatca ctaagcgctc tgcttcttct ttatgcaatc ctctactcat
    22321 cagatagtac atttcttctg gatcaactcg tcctacactg gcagcatgtc cagcagttac
    22381 ttcgttttca tcgatcaaaa gaataggatt cgcatcacca cgtgctttat cagaaagcat
    22441 caagacacga ctttcttgtt gggcatctgc accttttgcc cctttcaaga tgtggccgat
    22501 accattaaag gtcaatgttc ctttttctcg gatcactccg tgttgcaaaa tatgcccgat
    22561 cgtatgaggg gctttattgg ttactcgtgt atcgattcct tggatttgtc tgcccgagct
    22621 gattgccacg attttgactt ctgcgtgtgc accttctcct gctaaatctg agtcaaagtc
    22681 agcgatcaca tgaccgtcat tcatgacacc aattgcccag tcgacagaag catctcgtaa
    22741 aatgtgccca cggcgattca tataagaagt aatattttct cctaattgat ctacggctga
    22801 atatttgatt tttgaaccag ctttagcgat gacttccacg atgatattac ctgatgattt
    22861 tgctacttgt tctttggttg tctggaaacg ttccagataa gaaaattcac tatgttcatc
    22921 tgccacgatc aacacatgct tgaagaagtg ctcatcggat gcgccatctt gaatgaataa
    22981 ggactcgagt ggttcatcga tcacgacatt tttaggcaca tacaggaaaa taccgctgtt
    23041 cataaaagct acatggagtg cagtcaattg gtcttcgttc atgtttactg cttttttcat
    23101 ataatattct tggactagtt ctggatattc gatcattgca gtgaacaagt cggtaaaaat
    23161 gactcctttt tctgctaatt ccacaggcag ttgttcaaaa atcgtccatg aaccttgttg
    23221 gacaagcaca ggattgtctt tcattgcatc aaatgccgga atcgtgcctg tttcactgat
    23281 ggtttgtgtt tctttcacat caaataacga ccagcgatgg aatttcaccc gatcgatatg
    23341 cggcaatggc agttcatctg ctttcgctag tgcgtcttga cggaaggttc tcatccaatc
    23401 aggttcgcct ttacttaaag aaaaatcatt taccgcgtca agataatcga gccagttttt
    23461 ttctttcatt cttgctcccc cttacgcttc ttcttcttta tagtcgattc ctaattcttt
    23521 actgatacca gcgtagcctt ctgcttccag acgtttagct aggtcagcat tccctgtcat
    23581 gactactcgg ccttccatca tgatgtgaac gacatcaggt gtgatatagt tcaataatcg
    23641 ttgatagtgg gtgatgatca atgcaccgaa attgtctccg cgcatttcat ttactccttt
    23701 agcaacgact ttcaaagcat cgatatccag acctgaatcg atttcgtcta aaattgcaaa
    23761 tgtaggttct aacatcaaca attgcaggat ctcattccgt ttcttttctc cgccagaaaa
    23821 accttcgttc agataacgtt cagccatttc ttctggcata tttagtaaag ccattttttc
    23881 atctaacttt ttcaaaaatg ccataacaga aattttgtcg ttttcatcac gtttcgcatt
    23941 gatcgctgca cgcatgaatt ccgcattcgt gatacctggt atctcacttg gatattgcat
    24001 agctaaaaat aagccaagtc gcgcacgttc atctacttcc aagtctaaaa catttttccc
    24061 gtcaaacaaa atttcgcctt ctgttacttc atagtttgga ttgcccatga tagcggcaga
    24121 taaggtagat tttcctgtgc cgttaggtcc catgatggca tggatttctc cggttttcat
    24181 tgttaggttt acgcctttaa ggatttcttt ttcctcgatc gacacatgta gattttttat
    24241 ttctaagaca gacatgcatt atacccccaa attctctttc gttctcactc attttagact
    24301 aattgagaat gaagcgcaat gaccttcctt actaaaatca aatatctttt atttattatt
    24361 aaacaatttt gatttctaaa ttagaatagg tataaaaaag atgagaaata agccattaca
    24421 gacttatttc tcatcttttt tgttccatac cttattttgc atcagccttt actggtttaa
    24481 cagagccgcc ccatttgtct gtgatccatt cttggacttc tttgcggtga agcacttcaa
    24541 tcagtttttt gattttgtca ctgtcttcat cacctttacg agtcgcaata atattcacgt
    24601 aaggagagtt gtccttttca agcaatacgg catcttctaa tggattcaac cctgcgccgt
    24661 aagcgaagtt tgcgttgatt gcaactaggt caccttcatc attttggtac gtagaagcta
    24721 aaagtgatgg gtcgatcgta tggttgaact tcaagttctt agggttttct tcaatatcat
    24781 cgaatgttgc tgtttccaga tctactccat ctttgacttt gactaagcca gcatcttgta
    24841 gaattgtgat gatgcgtccc cagtctgatt cagaatttga agtaatgatt gttgcaccat
    24901 cttttaattc ggaaacatct ttgacttttt tagagtacaa gcccattggt tcgatgtgga
    24961 cagcaccggc attgacaaag tcatatcctt tttctttgat ttctttgttc aaataaggaa
    25021 tatgctggaa gtaattggca tcaatgtcgc cttcttccaa agattggttc ggtaatacat
    25081 agtcatcgaa tttgacgatt tcaagatcga ttccttcatc tttcaataat ggtttcacat
    25141 gttccaaaat ctctgcgtgc ggtgtcggtg aagcgccgac tttcaatgtc gtgtcgtctg
    25201 ctttatttgc tgaatctttg ctgtcgctgt tgttaccgca tgctgctaat cctacagtca
    25261 gcaaaagcgc tgctgcaaat ccaaaaattt tctttttcat atcctacatc cccctatatc
    25321 ctattatctt ttgtctgttt tcttagttaa gaaatcaccg attccttgaa cgataaatac
    25381 gattagtaaa ataattaaag tcgcaacgaa tgtcactgtt agattaccac gctggtagcc
    25441 ttcctgccaa gcaagacctc caagtccacc tgcgccgatc gcgccggcca tcgcagtaaa
    25501 tccgatcatc gagatcgttg taactgttaa tccagaaatc aatgcgggaa tactttcagg
    25561 tatcaagacc ttccagatga tttcccagta acttgctccc attgcatctg ctgcttccag
    25621 tacaccaggg ctgacttcac gaaaagcaat ctctactaga cgggcataaa atggtgctgc
    25681 ggataaaaca agagctggaa tggctgcttg cgcacctagc atagtgccta cgattgcttt
    25741 agtaaatgga atgatcaaaa ccatcaggat cataaatggc gttgaccgga aaatattact
    25801 gatgatcgat acgattccat aaatgattcg agcatacggt ttgtttttgc gtccaataga
    25861 gtaaaggatc aatcccaaaa ctaatccgat gatcgtcact aagaccattg agattaatgt
    25921 catatatagt gtatcaagga tagcctgatt catcgtttcc gggttgattt gtttaaaatc
    25981 tagatatgtt tctgaaaagc ttttatccac gatggatcac ctcaattcct actccttgtt
    26041 ctttgatgaa cacttctgct tgatctaatt ctttggtttc accagtcatc aagacagtca
    26101 atgaaccaaa ggaaccttct ttcacttgtt tgatctttcc atacacaacg ttgacatcga
    26161 tgttatagcg tcgaatcgct tgggaaatga ctggctcgtt ggcatttctt tcgctaaagg
    26221 tcaaagttgc caaacggccg cttggatttt ctttgatgag ttcagctaat agttctgtcg
    26281 aatcagcttc tggttcgatg tcttgttgta caaagcgttt ggttacttct tttttcggtt
    26341 gtctgaagac agtcaatacg tctccttctt ctacgacttt ccccaactcc atcacagcga
    26401 ctttgttgca gattttgcgg atcacattca tttcatgtgt gatcaagacg atggtcaaat
    26461 tcaatttctt attgatcgct aacaataagt ctaatacttc ttctgttgtt tgcgggtcca
    26521 atgcgctagt agcctcatca caaagcagga tctcaggatc atttgccaat gctcgtgcga
    26581 taccgacgcg ttgtttctgt ccgccggaaa gttgtgaagg atatgcttgt ccgcgtcctt
    26641 ctaacccgac taaacgcaac agttcttctg cccgttcttt acgtttgctc tttggtacgc
    26701 cagctaattc cagcggcagc tggatatttt caagtacagt tcttgaccat aacaggttga
    26761 aatgctggaa gatcataccg attttctttc ggaatgtacg taattctttg ttttttaatt
    26821 ttgtaatatc ttgatcgttt acgatcactt cgccgctcgt gggaagctca agtccattca
    26881 ggagacggac taatgtactt tttcctgcgc cagaatagcc gacgattcca taaatatccc
    26941 ctttttcaac gtttagtgaa acgtcattga cggcttggat gtttccgttt ttcccattga
    27001 atgtcttttt gacattcgtt agttggatta atgacatgcc attcacatct ttctcttata
    27061 atttttcaaa caaaaaagcc ttcgccctct tgccttaagc aaaaggacga aagcttgaac
    27121 ttccgtgtta ccaccttgat ttaccgttac ttcgcaataa cagcctcttc cagtacttgg
    27181 acttagcttc tttccgtata ctgtcgcgct gtaacgggca ctcccgtagt aagtttgctt
    27241 acgctcgctt actctgctct aagaccatct tccatctcat tttcgttacc cattttcagc
    27301 aaacagggct ctcttcaaac gtccgtgaca tgtactcttc ttttcaaagc tttctcgttt
    27361 catataggat tgcttatgat tatagcaact ggaagaatca gtgtcaactt ttttcaagtg
    27421 attttctgat tttataattc gtcaaattct tttggatcaa catctttgaa acttaagatc
    27481 cacgcattca tttcatctga atcattcaag atattgatat cttcaccgat tttatcatta
    27541 acagaagaaa ctgtacccgt tagcgggctg gcaaattcat tgaccgcttt ttccgcttca
    27601 acttcgatca gaggttcatc tttttttagc gattgtcctg cttttggcag attggcaaac
    27661 gtgatgtctc caagttcttc ttgcgcttca tttgtcaaac caacacagta ttcttttcca
    27721 ttaaataaga tccaaaggtt gtcctttttc ttcaaatgct tgcctgccat tttatatcag
    27781 ttctccttca taaatttttt cattgaatcc aattgcaaca agctggtcct ctcgtaagat
    27841 cagtggtcgc ttgatcagca taccatctgt cgataagact tggctcgctt ttgaagccgt
    27901 aaactcgcct acttgatctt tcagtccaag ttcccgatat ttcatcccgc ttgtgttgaa
    27961 caggcgacgg attggtactt gactcgtttc tagccaagtc tcaatcgttg aggccttcgg
    28021 tacttctttg atcatatcaa cggtttgata actaacgcca tgctggtcta accacgcttt
    28081 ggcttttttg catgttgaac atttcgggta ccagtaaaag gtgtacaatt ttcttacctc
    28141 cttgaagatc ttgcaggtgc ttcattttgg tatctcatcg ataatatcaa cccgataccg
    28201 atcatgttcc ctaaaatcga ggatcctccc tgactaatga aaggcaatgg gatccctgtt
    28261 aaaggaagta atccgatatt tgctccaata ttctcaaaga catggaacag cagcatcatg
    28321 atcaaaccgc ttgcgatata ggcgtaaaat tcattattcg tatcgaaaca cacccgaatc
    28381 atacgataga ttaaaataaa atacaagaaa atgacgaatg caccaccgat aaagccgaag
    28441 ttttcaccga taacggtaaa aatcatatcg gactcacgaa caggcacata gatattgctt
    28501 acgttgaatc ctttaccaaa caagccaccg gtaccaattg ccaaaagtcc ttgggccggc
    28561 tgataactca ttcctgacgt atcatggaaa gggtccagcc atgaatcaat acgtgcaaac
    28621 tgataagatt tgaatccaac tttataaagc aagtctcttc cgccttcagt cgtaaccaag
    28681 aagatcgttc ctgctccaac gactactgcc aagattacta caggtgcgat gatgcgccaa
    28741 gagattccag acatcaggaa aatcccgcca aagatcgcaa gaaagacaag catcgttcca
    28801 aagtcatctt gggccatgat caatgctaaa accggtattg tcacgatcaa gagcttccca
    28861 atcagccaaa agtccgattt catcgtccgt tcttttactt gcgtattatg tttagttacg
    28921 atcagtgcca tcatcaagat gtacgcaatc ttcatcaact ctgctggctg gaaagtaaac
    28981 gcaccgaatc gaaaccagtt tttagaccct gttgaagcag ctaaattctg atcatagaat
    29041 ttgagcaaga gtcccattac cactaacccc aaaccataaa ggtaaggagt cagtttccag
    29101 atccatttgg aattcaaatg catgatgaca acgattgctg ccgctccaac taaataccag
    29161 acaacttgca tccctacgcc tttcaagact gctggattgt tcggatcatg gctaagtgct
    29221 acatataatg agagcagacc aatcaaacat aacagaaaga ctggtaaaat cacgccataa
    29281 tcaatacgat tatcgttcga agcttttaat tttttttcca tgtttcatcc cttttcttaa
    29341 aaaccatact gctgattatt atagcggtcc agttagataa tggaaagtct ttccatagac
    29401 atttgggaaa aattaacttt ccgtttgtgc agttgcttga gcaaatgcag tacaaaagcc
    29461 tactgcttat ctttagtgta tcatagaact ttttttgata catgcaatta tctaccagct
    29521 gagattctcc tcgctgtgag aagtttctgt gctttcattg tatcgaagga tctcttcggc
    29581 aatctctagc agctgatcag aaaccagatt ggttcgttgg atgaaataaa aatatcgatg
    29641 gaaattcaac ggagtcattg gcacattttc tggaacagag cgcttagaca aaatcgagcg
    29701 accgattcct tccttcagca acgcaacaat tacatcgtta cttttcacga tcatcttctt
    29761 atcttggata ttcatctctt caaaataacg ctgcgtgtaa tgaaaaacac cagagtttgc
    29821 ttctcttaca agccacaatt tgctttttgg gtccccagcg atgactagct ggtcttccat
    29881 caaagagtat ctttgtacgc cggaagtctc taatggcttt tcgataaaac caaaatcgga
    29941 ttcatgtttc gctacttgtt ctaatgattc atgagaattc gtgatttcga tctcaaaatg
    30001 cactttagga aaccttttga tcagatgttt catcaactga ggcaaaagat agacagcaaa
    30061 ggtgtgggaa gcaacgatcc gacatacttc ttcttcattt tgttcattga gcaatttttg
    30121 ctgagtttct tcccattctt ctaacaaaac cagtgtccgt tcatataata agtcggcttg
    30181 cttagttgga agcatctctt ttcttccgtt acgttgaaaa agatcgatat tcaattcccg
    30241 ctccaactgt ttgatttgat tcgagactgc tggttgagat aaaaacagta cttctgctgc
    30301 tctggaaaaa tttcttgttt catatactgc tcgaaaagtc gttaataact taaacatggc
    30361 accacttcca taacttttta tgataattac tataaaaact atttgattta aatatacaaa
    30421 aaaccatcta taatagaaat gtacaacaaa acagttgctg atccaactag taacttagat
    30481 gaagacaatc agctgtctgc ttgctcgtaa tcatacattc ctattcattt tacaatgtat
    30541 tcgttcatct tggtactaaa atctattaaa taaaaaggag aatattatat atgaagaact
    30601 ttttgaaaat tattccgatc ccgatctgcg ggttgatcct tgggttagct tcccttggaa
    30661 atctgtttaa agactatcac catgttgctt taggcaatct aactggcggt atctctatga
    30721 ttcttatgat attcattttt gttaaattgt ttatcttatt cgaacacacg aaacaaacgt
    30781 tgcatgatcc aatcatcgcc tccgtttcac caacttttac gatgtcactc atggttattt
    30841 gtacttattt tgtttcatat gaaagtattg caccaattgt caaattcatt tggctgtgcg
    30901 ctgttatctt ccaagtgatc cttgtctttt atttcaatta tcaccatgtt atcaaagctg
    30961 atctttcaat tgaagcaatt tatccaagct ggtttatcat ttacgttgga tttggggtca
    31021 tcactgtaac tgccgggaac ttcttcccac ttattggaaa aattttcttt tggatttcat
    31081 tgatctgcta tgcgttctta ttgccgatca tcatcaaacg tgtctttttc gtaagaaata
    31141 tggctcatcc gacacgtccg ctgattacga tcgttgcagc acctggttct ctttgcctaa
    31201 caggttatct taaaaacttt gctgaaccta atttttatct tgtcgtagca ttgttcgctt
    31261 tatctcaagt attgtatttc gttgtgctgt ctatgcttcc acgtttgttc aagcttcgtt
    31321 tttatccaag ctatgcagcc tttacattcc cgcttgttat ctctgcaaca gctatattta
    31381 ctactgttca ttacttcaac agtttaggta tctatagtag cgtattggat ttcttaaaag
    31441 ttgcggaatg tctgtttgca agtgtagttg tcttctacgt tcttgctcat tacgtacgtt
    31501 tcttaattaa agaacatagc aaacatggaa aatttgtttc taaaaaaatc gcttaataat
    31561 cgattcaata ggttgtgaaa aagctgttct gcatcaagta ataagaacgg ccaaacaaaa
    31621 acagctgttc atgtttttgt actggttggg cttattaccg cagatgcaag cttttgaaca
    31681 ccgtttacca agaggtcgta a
//
LOCUS       DACCKU010000025        31390 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.025, whole genome shotgun sequence.
ACCESSION   DACCKU010000025 DACCKU010000000
VERSION     DACCKU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 31390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.025"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(54..500)
                     /locus_tag="IVN13_001080"
     CDS             complement(54..500)
                     /locus_tag="IVN13_001080"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP7011582.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRIRGITVATGRFGADMSVSLVNDGPVTIIIDSKNK"
     gene            complement(511..2724)
                     /locus_tag="IVN13_001081"
     CDS             complement(511..2724)
                     /locus_tag="IVN13_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP7011583.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            complement(2957..3721)
                     /locus_tag="IVN13_001082"
     CDS             complement(2957..3721)
                     /locus_tag="IVN13_001082"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP7011584.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            complement(3722..4669)
                     /gene="prmA"
                     /locus_tag="IVN13_001083"
     CDS             complement(3722..4669)
                     /gene="prmA"
                     /locus_tag="IVN13_001083"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP7011585.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQIVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            complement(4682..5170)
                     /locus_tag="IVN13_001084"
     CDS             complement(4682..5170)
                     /locus_tag="IVN13_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP7011586.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            complement(5136..5807)
                     /locus_tag="IVN13_001085"
     CDS             complement(5136..5807)
                     /locus_tag="IVN13_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP7011587.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            5945..7231
                     /locus_tag="IVN13_001086"
     CDS             5945..7231
                     /locus_tag="IVN13_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP7011588.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            7303..7497
                     /gene="ssrS"
                     /locus_tag="IVN13_001087"
     ncRNA           7303..7497
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IVN13_001087"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            7575..7859
                     /locus_tag="IVN13_001088"
     CDS             7575..7859
                     /locus_tag="IVN13_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011589.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            7932..8411
                     /locus_tag="IVN13_001089"
     CDS             7932..8411
                     /locus_tag="IVN13_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7011590.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            8634..8777
                     /locus_tag="IVN13_001090"
     CDS             8634..8777
                     /locus_tag="IVN13_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP7011591.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            9355..10938
                     /locus_tag="IVN13_001091"
     CDS             9355..10938
                     /locus_tag="IVN13_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7011592.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNPYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDESTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(11375..12559)
                     /locus_tag="IVN13_001092"
     CDS             complement(11375..12559)
                     /locus_tag="IVN13_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP7011593.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(12584..13591)
                     /locus_tag="IVN13_001093"
     CDS             complement(12584..13591)
                     /locus_tag="IVN13_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7011594.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            complement(13763..14122)
                     /locus_tag="IVN13_001094"
     CDS             complement(13763..14122)
                     /locus_tag="IVN13_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011595.1"
                     /translation="MPYGQKPISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            complement(14094..14465)
                     /locus_tag="IVN13_001095"
     CDS             complement(14094..14465)
                     /locus_tag="IVN13_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP7011596.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKTDQ"
     gene            complement(14482..14784)
                     /locus_tag="IVN13_001096"
     CDS             complement(14482..14784)
                     /locus_tag="IVN13_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011597.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            complement(14801..15271)
                     /locus_tag="IVN13_001097"
     CDS             complement(14801..15271)
                     /locus_tag="IVN13_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP7011598.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            complement(15246..15524)
                     /locus_tag="IVN13_001098"
     CDS             complement(15246..15524)
                     /locus_tag="IVN13_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP7011599.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            complement(15521..16570)
                     /locus_tag="IVN13_001099"
     CDS             complement(15521..16570)
                     /locus_tag="IVN13_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP7011600.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            complement(16527..17456)
                     /gene="cpaF"
                     /locus_tag="IVN13_001100"
     CDS             complement(16527..17456)
                     /gene="cpaF"
                     /locus_tag="IVN13_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP7011601.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            17588..19342
                     /gene="ade"
                     /locus_tag="IVN13_001101"
     CDS             17588..19342
                     /gene="ade"
                     /locus_tag="IVN13_001101"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP7011602.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            complement(19440..20102)
                     /gene="trmB"
                     /locus_tag="IVN13_001102"
     CDS             complement(19440..20102)
                     /gene="trmB"
                     /locus_tag="IVN13_001102"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP7011603.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(20150..20929)
                     /locus_tag="IVN13_001103"
     CDS             complement(20150..20929)
                     /locus_tag="IVN13_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP7011604.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            complement(21136..22347)
                     /locus_tag="IVN13_001104"
     CDS             complement(21136..22347)
                     /locus_tag="IVN13_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011605.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            complement(22340..23077)
                     /locus_tag="IVN13_001105"
     CDS             complement(22340..23077)
                     /locus_tag="IVN13_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011606.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDTAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            23215..23649
                     /locus_tag="IVN13_001106"
     CDS             23215..23649
                     /locus_tag="IVN13_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP7011607.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            23642..23986
                     /locus_tag="IVN13_001107"
     CDS             23642..23986
                     /locus_tag="IVN13_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7011608.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            24166..25176
                     /locus_tag="IVN13_001108"
     CDS             24166..25176
                     /locus_tag="IVN13_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7011609.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLKSSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(25245..26189)
                     /locus_tag="IVN13_001109"
     CDS             complement(25245..26189)
                     /locus_tag="IVN13_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7011610.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(26173..28893)
                     /locus_tag="IVN13_001110"
     CDS             complement(26173..28893)
                     /locus_tag="IVN13_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7011611.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            complement(28890..30110)
                     /locus_tag="IVN13_001111"
     CDS             complement(28890..30110)
                     /locus_tag="IVN13_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP7011612.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSEMVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKIPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            complement(30391..31236)
                     /locus_tag="IVN13_001112"
     CDS             complement(30391..31236)
                     /locus_tag="IVN13_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7011613.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
BASE COUNT         9331 a         6451 c         5457 g        10151 t
ORIGIN      
        1 gtctaactta agcgacgtgt ttcatagttt ttactagtga aaaccagatt actttatttg
       61 tttttagaat caataatgat cgttacaggt ccatcattga cgagagaaac cgacatatct
      121 gctccgaatc gtccggtagc gactgtgatg ccgcgaattc tgattccttc attgaatgat
      181 tcgtataaag gaatagctgt ttctggtcgt gcggcttcca caaagcttgg tcgatttcct
      241 tttttcgtat ccgcaaacag tgtgaattga gagatactta aaatttgtcc tcccacatct
      301 tctacactaa tattcatttt tccttgttca tcttcaaaaa tacgcatttg tgcaattttt
      361 tttaccagat agtccacatc tttttgcgta tcggtttcat gaatgcctaa taaaacaagt
      421 aggccgtagc caatttttcc aatttcttgc tgatcgactg acacagcagc tttacttacc
      481 cgttgaacaa ctgctctcat atctgtcctc ctatccattc gtccggcgta cactgtagac
      541 atcaggtact gctttgattt tatccacgat actctttagg tgggataaat tctggatacc
      601 aaccgtcaaa tggatcgtcg ccattttatc tttattcgtc cgtgcttcaa cgctgttgag
      661 atttttcgtt agcgcattga ctgtttgaag tacatcattc aataagcctg aacgattata
      721 gccgtaaatt tccaaatctg catcatactc ttttcgtgta ttcgatgtat cttcccattc
      781 gacttcaatc aaacgttctg ctacatttgg tttgtcaggc tgaacattcg gacaatctcg
      841 acgatgaatg gatatccctc tgcctttagt gatataacca acgatatcat caccaggaat
      901 cggattacag cagcgactga tacgaattag taagttttct accccttgaa tgacaacgcc
      961 accttcatga cgtactttca ttttgtcagg ttcttttttc ggctgattca tgatttcttc
     1021 tgcttcttgc ttttgctgct cgattttttg ttctttacgt tctttttcag tcagacgatt
     1081 ggccatcgtc aaggggctaa cttctccata ccctacagct gcatagagat catcttctgt
     1141 ttgataatta aaacgatcga gtgcttcttg cagtttgttc ttcgtcaaaa tatctttagg
     1201 cgtaaatcct agatctgtaa tacatttgac cacggattcg tggcctttga tcacattttc
     1261 ttctcgatct tgggctttga agaaacgttt gatcttattt cttgctttgc tagtagcaac
     1321 taatttcaac cagtcgcgac ttgggccaaa tgaatttgga gaagtcatga tctcaatgat
     1381 atcgccgttt ttcaatttgt aatcaagctg caccattttg ccatttactt ttgcaccagt
     1441 ggttttgtta ccgatatctg tatggatact gtatgcaaag tccaatggtc cggatccttt
     1501 tggtaactca gtaacgtctc cctttggtgt gaagacgtag actttatcac taaagatatc
     1561 tccttttacg ccttccataa attcagaagc atcatagctt tcgtcttgaa gttcgagtat
     1621 ctcatggaac cagcttaatt gtttcgtcat accatctggt tctacttttt cattttttcc
     1681 ttctttatag gcccagtgtg cagcaacccc gaattcagcg atttcatgca tttcctgtgt
     1741 tcggatttga atttctaccg gatttcctgc cggaccgatc acggtagtat gcaaagattg
     1801 gtacatatta gcttttggca tggcaatata atccttaaat ctgccgggca ttggtttcca
     1861 ttttgtgtgg attgctccta atacagcata acaatccttg attgaatcta cgatcactcg
     1921 aatagctagc aagtcataaa tttcgttgaa ctgctttttc tgatctttca ttttacgata
     1981 gattgaataa atgtgttttg gccgtccata gatttctgca aaaatcccca gctcttctgt
     2041 tgctattcga atatcttcaa cagtaccact tacgtatttt tcacgttctt ctctcttcgt
     2101 ctgcatcaag tggacgatac ggtaatactg ttttggattt agataacgaa gtgctgtatc
     2161 ttctaattcc catttgatcc ggctgatccc tagacgatgt gcaagaggcg catagatttc
     2221 taaagtttcc tgagcgatcc ttctttgctt atcttctcgc aggtgcttca acgtacgcat
     2281 gttatgtagt ctatcagcta atttgaccat gataactcgt aaatcttgag ccattgctag
     2341 cagcatcttt cggtggtttt ctgctagctg ctcttcgtgg gatttatatt ttattttccc
     2401 taatttggtt acgccgtcca ctaacatagc aacgtcatcg ccaaattctt ctctcagatc
     2461 ttctaaagtg atttctgtat cttcaacaac atcatgtaag aagcctgtag ccactgtatg
     2521 gggatccatt tttaaatctg ctaatatacc tgcgacttgg ataggatgaa taatgtaggg
     2581 ttcgcctgat tttctaaact gtccgtcatg tgcagcagtc gcgtattcgc atgctttctc
     2641 aacaaatgct acatgttcgg gacccatgta ttgactaacg agctttatga caccgggtcc
     2701 cgttaaaatt tcttcttttg tcacaggaat cgatccccct ttcttttttc ggtaaaacgg
     2761 gaatcgggac aaatatctgt ttgataattg tcccaaaatc cgtgcagtta tacgtttttt
     2821 tacggaaatg ttcgcttttt aaaagctttt tcaaacgatt tttcgtatcc attgtataaa
     2881 taattgtttc tattatacaa attgttgact attttttcaa ttctttccgt cgatactctt
     2941 ttattaatga agaacgttag ctgaaagaag gattcattaa ttcccattga tagctagcag
     3001 cacttaataa ataaagaggt gctgtttctg tacgaaggat tcttggtcct aatccacaaa
     3061 gtatcccttt gtgttctaca aaataatcga cttcatcgcg ggataatcca ccttctggtc
     3121 caaagacagc taagattcga ctatctggtt ttgtttcact aagaattttg gctgcatttg
     3181 atttttcgcc ttgctttgct gattcctcat aagcaaccaa gaccacgtca taatcatcta
     3241 gccgctcaaa taaatcttgt ttcgtttcca ataactctac tgatggcacg atctgccgat
     3301 gggattgttc cgctgcctct tgagcaatct tttcaagccg ttgttgtcgt ttcatacgtt
     3361 tttttgcatc ccatttaacg acagaggctg atgccgggaa accaagaaaa gcttgcgctc
     3421 ctaattcagt ggatttctga acgatccaat ccagcttttc ccctttagga tagccactcg
     3481 ctatggtaat cgaacttggc agttcctttt gattttcttc ttttttaatt tctttcagat
     3541 aaacagcctc ttcagtaatc tccgtgattt ctgcaacaat ggctatttga tcattgaatg
     3601 ccaaaaaaac ttcatcctcg ggttccatcc tcataacccg tacaatgtgg tgatagtttt
     3661 ccccttctaa aacaaaatag tcttttgatt catatgattc ttttaaaaaa taccgctgca
     3721 tctattccac ctctggtttt ttcaaaataa ttgcatacca gtctttttgc tggaaaattt
     3781 gatccacgat aaacccttgt tctaacatgg catccagaac tatttgcttc ttctcttcaa
     3841 tgattcctga aacgatcagc gtgcctgttt gcttcagtaa acgccaagca tctgggatca
     3901 tcaataagat gatatctgct aaaatgttcg caacgattac atcactttca atctcgattc
     3961 ctgtcaaaag atcgttagct gatacatgga catctttggc tatttcattc atatccatat
     4021 tttctttcgc cgaacgaacg gctacttcgt ctaaatcaaa ggcatagaca tcttttgcac
     4081 caagatattt tgctgcaata cttagcacgc ccgacccagt tcccacatct agcaatgttt
     4141 caccaccacg caagactgtt tctagtgctg tcaatgtcaa ggaagtcgtg ggatgagtgc
     4201 ctgtgccaaa tgccatacca ggatctagtg tgataatttt ttctagagga tcttctgtct
     4261 ggtagttttc ccagcttggc gcaaccgtca gcattcgcgt aatacgcacc ggatgatagt
     4321 atttcttcca ggctgttgcc cagttgcttt cctctacttc actaactgtt actttatttt
     4381 ttccaatagc tagaccaaac tcaggtaatt gtgtgatacg atctttgata aaaggcaaga
     4441 tttccggtaa gaaagtcgtt tccggaaaat aagccatgac tagtgcgcct tctttcagcg
     4501 atgtaaaatc atttttatct aaaatctcac catattgatc cgactgaaaa tgttcaacat
     4561 ctaaagaatc ttcaatcgcg acaccactcg cccctgcctc catcaaaata ttagcaactg
     4621 cttcgactgc ttcactttca gtttctactt ttacttcatt ccatttcata tctgtcactc
     4681 cttaataact tgggtacggt aaataagtat ctgcttcatt ttgctgtttt acttctacta
     4741 attctgctag ttgctgatta tcccagtgaa gctcgaatac agcttcgtca tcgtcttcat
     4801 ccaaaaaatc aagcaaatcg ctttgccctt gagctaatac ttcatttaga tattccacta
     4861 aagaatcagc tactgatttt gccaaccctt tttttccttc ataagggatt actgctagat
     4921 aattgcgttc atcaaaaaca gatttctgag gattaaaaag aagtacgccg tcttcaaatt
     4981 cgataattgg ttcttgcgaa acgatccctt ctgcatcatc aatctcaaat tgtgctttat
     5041 tttctgcaaa caagcgaaca acgatctcga tcgtatgatt ttttgtatcc caatctaaag
     5101 ctacatcata gtctgtcact tgtttttcaa ttatttcatc taaatatgtt aatagatcct
     5161 tctttttcat tttctttcct ccaaccccaa tctttctcga tttggttttt tctttgtaaa
     5221 gagatataag gatttcccga cacaacaaaa cgcaacggct tttctgtcca tacacctttg
     5281 tttgggatac cgattctagg caaagcattg atcttttttg gtgtcttttt tttctcaaaa
     5341 actaagcgta acgaactatc tgctatagac tgaccataaa gttcttgagg aattgctaat
     5401 gcttccacta attttcccgg accgttgctg atatctggac cagtttttcc gccacgattt
     5461 ttactcattt gatcaatcat tgcggcgggt tcgattgcac gtatcattac tccttgagga
     5521 atcccttctg gctgagtaat gatgttcaaa atccgatgag tatgcatcgt gtaaaggtag
     5581 atcgtacctg gtttttcata cattgcccgc acacgcggtg ttcttcttaa accatagcta
     5641 tgggcggcct cgtcttctgg acctaggtat gcttccgcgt ctacaatata tccagctaat
     5701 cttccttctg gtgtgtcatg ctccaaatac atccccaata aatcccgtgc cacttctggc
     5761 gtcgttttat tccaaaaaaa ttcatctatc ttttgtttat ccgtcatttt tctcacctac
     5821 tttatcatac acaatattag ttggataagc tatgaaaaca aaacaacaga aggctgttct
     5881 ttctaggcat cttgctaaat atgatataat taagggacgt aaaaacaaga aggaggaaaa
     5941 tcctatgcaa cagccattgg cttatcgcat gagacctcga acgatcgatg aagtcgttgg
     6001 tcagcagcat ctagtcgggg aaggaaaaat catacgccgc atggtcgatg ctcgcatgct
     6061 ctcttccatg attctatatg gacctcctgg tactggaaaa acgagtatcg caagtgcgat
     6121 cgccggctct actaattatg catttcgcat gctaaacgcc gctacagatt caaaaaagga
     6181 tctacaagtt gtagcggaag aagcaaaaat gagtggtaca gtgatcctct tattggatga
     6241 agtacaccga ttagataaaa caaaacagga ttttctgctt cctcatttag aaagtggccg
     6301 aatcatcttg atcggagcga ctactgagaa tccttatatt accatcaatc cagcgatccg
     6361 cagccgtaca cagatctttg aagtcaaacc attaacagaa caagacatcc agttggcagt
     6421 cgaacatgct ttaagggata aagaacgcgg ccttggacaa caagcaatcc agttagacga
     6481 agatgctctt cttcaccttt cgcgtgcaac gaacggcgat ttgcggagtg cattgaatgg
     6541 gctggaactg gctacactat cgactccttc agataaagaa ggaaggattc atcttacact
     6601 gtcgatcatt gaagaatgtg tccaaagaaa agctcttacc catgacaaaa atggagatgc
     6661 tcattatgac gttatttcgg cttttcaaaa atcgattcgt ggaagtgatg tggatgctgc
     6721 acttcattat ctcgcccgtt tagttgaggc aggtgatctg gcaagtattt gccggcgtct
     6781 gatggttatc ggttatgaag atataggttt aggtaatcct gctgctgcgg ctcgtacagt
     6841 gaacgctgta ttagctgctg aaaaattggg attgcctgag gcacgtatcc cactagcaga
     6901 cgtcgtcgtc gatctttgct tgtcgccaaa atccaattct gcctacatgg cattagatgc
     6961 agcacttgct gatattcgag agggcaaagc tggagatgtt cctgatcatt tgagagatag
     7021 ccattacaaa ggtgcgaaat cactcaaccg tggcgtgggt tatcagtatc ctcatcattt
     7081 tgatcaggct tgggtcaatc agcaatattt accagataaa ctgaagaatg ctcagtatta
     7141 tcagccaaaa gatacaggga aatatgaaca ggctctgggt caacagtatt accgtataaa
     7201 agagtggaaa aagcatcctc caaaaaacta gacaagaaaa cggttctgtt tctgcgagct
     7261 ctctcattgc tctttttaaa ttcatatgct actatagagg tgggaaatct gtgatgttcg
     7321 aattgtcctc tttgtgtgtt gaccgaacat ttttattgat atcttgggac ccgtcttgtg
     7381 tcactgctgg taacatgcct tatcatttgg tagttcgaag ctacgaccat gggacccacc
     7441 tgcttgaatt cgcgggatca atactacagg acaaacaacg gcattaacgg agattaccat
     7501 cttttaaaga ataaagcaat ttgctcttgg caaattgctt tttctatttg ttgcttattt
     7561 tggaggattt gttcatgatt aggattttaa tgattatcgc cactttagta ttattattcg
     7621 ttagctatta tttatttaat aaacaggata tcttttttgt attgatcaac aaaaatgata
     7681 aaaatcaagg attcctacaa ttttacggag ctgcatatgc tgttttaggc gtaatgggta
     7741 ttttaacagc ttttttcaat caacgcttta ttgccttggt ctttttactg atcgtaatta
     7801 ttgtttctgc caccttcagt atccgatttg caaaaaaaat tgctgaacca aagcagtaaa
     7861 taattctctt tttccctaaa gtgttctaca ataaaagagt acaatacgat tgacttgagg
     7921 agtgaatgct tatgttacaa caatacaaaa aaatcatggt tgccgtagat ggttctgatg
     7981 aagctgaatt agcttttaaa aaggcggtca atgtagctat ccgaaataac ggagaacttc
     8041 tattagcaca tgtcattgac acacgttctt tccaaaccgt ttcatccttt gatggaatgc
     8101 ttgcagaaca agctactgaa atggcaaagc aaacattggc agattatgaa agcaatgcga
     8161 agaaagctgg cttgaataat gtaacctctg tcgtagaata tggctcacca aaacaaatta
     8221 ttgccagaga aatcccagaa gataatcaag tagatttgat tatgttagga gccacaggat
     8281 tgaatgctgt agaacgttta tttattggtt ctgtttctga atatgttatt agaaacgcag
     8341 cttgcgatgt gcttgttgtc cgaactgatt tagaaaacca attgccagct aaagaccaca
     8401 ccaaagctta acacgtagac gacctgccaa ctatcggcag gtttttttgc acaaaaaacc
     8461 gtgataaaaa caaaagacgc ggcaagcact catcggtctt tataatgttt aaaataaagg
     8521 gaaaacagtc aatgagttca agtaaaataa aaaaaagtga acaccagggc gattagtcgc
     8581 cctggtgtgg tttaaaacga cctaagcatg ccaaagctta ttggttcgtc aatgtggaag
     8641 tttccgatga atctgatacg aaaaaatatg aagtatcgat ggcggatacc gattttatcg
     8701 ccaaactaaa attttatttc gggttcgggt accgcgagaa ggcaagtgct acattcggta
     8761 aatcattcaa gaattgatga aaggctaccg cttgccattg caaccacaac gatactcctg
     8821 aacgcgatgt tggtgagatt tccaaagaga agagctgttt aatgcaacgg tattaagcaa
     8881 atggcaaaag gaagtcatgt agacaaagtt ttttatcaaa caccatatga tttcgcctga
     8941 acgctggttt agctcagaat acaatgatgt ggtccacgag acattaccct cagtcagata
     9001 aaaaaagctc tctgacagaa ttacacatcg ataaatatta agtaaatgtc agcgctagtt
     9061 gaactttctg acactttcag gtacactgga aaagacaact acacgaagga gggaaaatac
     9121 tatgatgcgc atgactaaaa taacaagtaa tgaaaattac caacgatacc atatcgcatt
     9181 tagccatgca attggtttta cagttgctgt tgacgggaca agtttgtcca aaacagttga
     9241 attcacgtaa aatcaatggc catctcaagg tatttttctc atgataatct gagtttaaat
     9301 aacacaggcc atggataacc atggcctttt tgagtggtct caaggagaga gatcatgatc
     9361 atacaagcaa tttctattaa aaaaacaatc ggcgggaatc cattatttga ggatttatcc
     9421 tgtcaaattg aagctggtga aaaaattgcc ttagtcggta tcaacggaac tggcaaatca
     9481 acctttttac aaatcttgac cggcagagaa ggtgtcgaca gcggtgttat cagtcgcaaa
     9541 aaaggcttaa agatcgggac ggtcgaacaa gaacttacag tttccgaagc gaccgtcaac
     9601 ccctacttgc tccattctgc cgctgagata caggacctca aacagcaaat gagtcgctac
     9661 gaagcattga tgacagaacc agatacaaac ttagagaagt gtctggcccg ttacggtgag
     9721 ctgcagcatc gctttgaaga actaggcggt tacgtattag aagaccggat tgcagcgatc
     9781 ttgcaaggct tagggattcc acaccgtcag gaagcacggt taacagaact tagcggaggc
     9841 gaacgagtta aagtcgcttt agccaaaatt ttagcagccg atgccgattt acttttactc
     9901 gatgaaccga ccaatcattt agatcttaac agtattcgct ggttggaaaa ctatttacag
     9961 aatacaaaaa aatcggttct ggtcgtttct catgatcggc aatttctcga tcaagtcacc
    10021 acaaaaacat gggaactgga agaaggtgca ttgattgagt atcccggaaa ctacagccgt
    10081 tttcgagtct tgaaagaagc tcgcttggct gagctgacaa aaaattacga actgcagcaa
    10141 aaagaagtcc agcggttgaa agtcatgatt cgtcgttttc gtcagtgggc acatgaagga
    10201 gacaatgagt ccttcttcaa aaaagccaaa gaactggaac gccgcctggc gaaactgaca
    10261 ctcgtcaaac cgccacctcc cccgaaaaat cggctgcaaa gcttgtctaa tggtggaaaa
    10321 tctggcaaag aagttttcat cattcaaaac ttgcaccagc agtacgctga tcaagtacta
    10381 ttcaaagaca gcagttttgc agtctatcgt ggagatcatt tagccatcat cggcgacaat
    10441 ggtgcaggaa aatcaacctt actcaaatta ttactcggtg aggaaaagcc ttacggtggc
    10501 accatcaaat taggtagtag cttgcaaatc ggttatttgc cgcagcagct gcagtttgcc
    10561 gatcccgata gccgtttgct ggcttacgcc atcacattga ctggaaacga agaagtcgct
    10621 cgacggcaat tagccaaaag tggcttttat caaagcgatg tgggtaaacg gatcaaagat
    10681 ttatccggtg gtgaaaaaat ccgtttggcg ttgctgaaac tctttttaga aaaaatcaac
    10741 gtcctgattt tggatgaatc aaccaatcac ctcgacagct atgctcgaga agaaattgaa
    10801 gaaatgctgc aagactatca aggaacgctc ctagcagtca gccatgaccg ctactttttg
    10861 cagcaacact tccaagaagc actcgttatt gatcaggagc agatcagccg ccagccgctc
    10921 ggccaactga ttgactagga cgttttttcg caacaaaaaa cttgattttc atacctgtgc
    10981 tttcacgatt caggcaaagt acaggtggaa aatcaagttt ttattattcg atcaatgaat
    11041 gtttcggatc ggttttctag ctaaacataa agcgaagaat aaagtcgaca gcccacgagg
    11101 ctgatctaaa aatgtttttt ctttttacat atgtaatgtc ccttataaag atgaatgaaa
    11161 ataaaatatt gggattttcc tgaaaatcaa taaataaaat gccagtcttt tcccttttga
    11221 ccacttttcc aagcaaaatt ccctaagaag atcctgtaaa aaagaaggga aattgaagtt
    11281 aaattaccat tttgcggtct tctccgcaaa aaaaacacat gcaagaattc cctcttgcat
    11341 gcgtttcatc ttttatccta acgaatgctt tggcttagcc gcgcagacgc tccacatcac
    11401 gggcaatcat tagttcctca tctgttggga tcactaatac tcttactttt gattcttttg
    11461 tagagatatc tacttcttca ccacgcaatt cattcaacgc tggatcgatt tcacaaccaa
    11521 accaagacat acctttgatg atttcactac gtacatgtcc gtcattctcg ccgataccag
    11581 cagtaaagac aattgcatca acaccattca tcgttgtcac ataaccgccg atgtattttc
    11641 ggatgcggtc cacgaaaatg tcataagcta aacgaatatg ttctttttca tagttctttt
    11701 caagatcacg catatcacta gacaatcctg ttaggcctag cagaccagat ttcttgttca
    11761 aaacattgat catttcatca atatcaatct ctaatttttc cattaaatat gggagaacag
    11821 ctggatcgat atcacctgaa cgtgtaccca ttgtcacccc agctaatggt gtgaatccca
    11881 tagaagtatc catggattta cctccatcga ctgctgtgat cgatacacca tttcctaagt
    11941 ggcaagtgat gatttttagt tcttcgatcg gtttgttcaa taattttgcc gcacgttccg
    12001 acacataacg atgacttgtg ccatggaagc catattttct tactttgaaa tcttcataat
    12061 aagacaccgg taagctatat aaataatttt gctttggcat tgttgagtgg aaagatgtat
    12121 cgaatacagc cacactcaaa atgtcaggta aaatcttttt aaacgctttg atccccatta
    12181 agtttgcagg attatgcaaa ggagcaaatt ctgctaattc agcaatgcgg tccattactt
    12241 tctcatcgat cactactgaa tccccataag tttctcctcc gtgaacaaca cggtgcccta
    12301 ctccggtgat ttcatcataa gagcttaaaa tttgcagttc aatcaactga tctaacagca
    12361 tttttactgc tacttcgtgg ttttcgatat ccatgatttg ttcgaatttt tgattatcgc
    12421 cgtacttgat ggtaaaaata gaatcttgta aaccgatacg ttcgacgatc cctttcgcaa
    12481 ctacggtttc ttctggcatt tgatataatt gccatttcaa acttgaactc ccagcattga
    12541 ttgcaattgt tttagacata tgactctcct tctcgttgaa tgattacaga gcagatgatt
    12601 tccagttttt aaactcgttg aaaaattccg tcaccttctc tggttctttt aatgaagcaa
    12661 gtttcacaag caagacttct tttacttgtt gtgcttgtgg tccttttttc tgtaagatca
    12721 aaatactttt ttgcgattgt ttattacgga acaattcatc tggcaattga atcattcctt
    12781 gaagataacc ttcttcttta aaccattttt tgatttcttc actttgatcc gtttctaaaa
    12841 atccactcgg catcaaaaac aaaccaaaac catccggctt cacatatttc attgattgct
    12901 ctagcaataa atgatgtgca taactatggc cttcttcggt acttgttaaa aactcttttg
    12961 ctttttcgtc attcggataa taaccgattg gcaaatcgct gattgcaaaa tcgacaggat
    13021 ctattaataa ttcctgcaac ccgtcttgat gaaagtattg aacatttgct tgtgtcaaat
    13081 cacttgtaga agctgcgaca gccaacaatg tatcatcaat atccacacca attccttctg
    13141 tttggtaccc cgcattcgat aggtttaaaa gaactgttaa taacaagttg cccattcctg
    13201 ccgcaatatc cagaatctta accggtgctt ttttatttgt gtatagttgt tcaataagaa
    13261 aaacaaataa aaaaccgata ctatcaggag tgagttgatg gttcgcctgc aaatgttctg
    13321 tctggctgcc cttcaataaa agtaattgag acagtctgcg ccattcttct ggctcgaaag
    13381 aaagtttctt caactcttca taaagagctg tgatccgttg cgttgtctct tttgtcggca
    13441 ctccatccac cacacgtact tgataatcat ctattaaatt ttctgcattt tcgacatagg
    13501 cttctaaaaa ggaagttccc aatgcattct gtaaaagctg aatagcttcc aggttttgat
    13561 taaacccttg ttccattttt tctggtaaca tggtgcacct catttctaga tatattataa
    13621 caattttttc acctgtttaa ttctatcgaa caattgacaa aatttcaatc gttttcactg
    13681 ttttttattt gtgaaacggc aaacattttt ttattaaaaa cagtgttata acagaaaaga
    13741 gggcgttttc acgaacattc catcattctg tactagtatc tgtatcattt tttacttctt
    13801 ctgtgaaccg aaagataaac ggtgagactt ccacctctaa gaatagggac tgttgtaccc
    13861 actgatatgt tacagatcca gttgtgtagt aaaaaactcc ttccgaactt tcggcaagcg
    13921 tagactgttc cgataaagcc atctgcttca ttatttttgc tatataataa tttttgattc
    13981 gtatattaag atccattgac aaacggtagc tttctaatgc tgccatcaat aaaaacgtac
    14041 ataaaagaaa taaaagcaag acagaaaata aaatagagcc cttataacat tttctactga
    14101 tcggtttttg tccatatggc atatccatct tctccatttt caaagtgtac ataaaaagaa
    14161 attgtgtgtt ctttttccaa taattgaagc tcagtgatac ttgttaacat aggttgatgt
    14221 ccgccggaag ttcttctttt acgtatcatt gcctgatatc tttcgatgat atactgttta
    14281 tcctcgatcg tgtagtgcag ttgctgattt ttcacagaga cattgtgact gctcttgagt
    14341 tcttcttcta attgtgctaa aaatattagc cactcttttt gatctttacg ctgcaaaaat
    14401 tcacttactt ggatggtttg ctgaatcaaa agattgaatg tgagcaatag actgctcaat
    14461 atcatcagtc caattagaca ttcaatcagc gtaaatgctg gaattacttg ttttttcaat
    14521 actgatcgct tctttctcca tattgacaaa cactttttcg attactccat tattatcgac
    14581 aatttggata tcgtatggga tcatttcttc ccttcgctct ggtcttttgt gatacatccg
    14641 ctgcgtcttg atatcttcat aaatcattcg aataaatagc agttcctccc cacttttctt
    14701 ttctgcctga agcaaaaaca gctggccaaa gctcactaag tagacacaga cagaagcgat
    14761 cataagcgct gtaagggact ccaacaaaac aaatcctgtt ttattcaatt ctttttgtat
    14821 agcgaccact ccctatctgg aattgatacg taatcgtccg ctctttttct tcccaataaa
    14881 attgataagc ttttagtgaa ctttcattcc cggttctagc agcaaaagta atagaagcat
    14941 ggcgtttcaa ggtaatctca tcaggaatat ctaatacttc ccaatttatt ttatctggat
    15001 tcggaacatg aaaaatgatt tgattctctt cactattcca ataaaagcct gtttggatct
    15061 gattcacaat agcgattttt tgcgtagaat agattcgttt ttcaaattgg ccgaaaaatt
    15121 gatagactgc caattcattc ttccatgcag aaaatgcgag aactggaaaa gaaaggatcg
    15181 ttgtaagtaa taaaatcagt aagagtgatt caaacaacgt atatcctgat gctaaaacct
    15241 taccttcaag gccgctgata gatttctgca tgttctcgtt tgatatagcc ttcattcaac
    15301 aattcctcca cagtaggaac tcttccatta ttttgaagcg catagagctc tttttgtgtg
    15361 tctaccaact ggatgatcgc ttcacgactt tcttggtcta catgattcct ataacgcgac
    15421 aaattaggga cgaacaacaa cactaataca ctgatgatca gtaagacgat tagcatttcg
    15481 atcaatgtaa aaccgctata ataagatatt tttctctttt tcatggcaaa acctcctcta
    15541 tgcttccgta aacaggtaat aaaacagcgg cataaactaa taaaatcaaa gccgctacgc
    15601 ccaaaaaaat gattggctga ataaaatgca tccagtgatg aagacgtttg aaaagctgtt
    15661 ttcttgtcca ttcgctgtaa aaaatcagtt ctttacctaa acttcccttt gcctctccct
    15721 gtaaaacaat tttactgaat tcttctgata gaaaacgata attctggaac tgctcagcca
    15781 atgacatacc tgcctccaat ccttgaatca acttagaggc aagatgctga atcagacttt
    15841 cttgtttcgt ttcctttaag cagactatga tttgccttaa ttcgaaacct tcatgaaaaa
    15901 gctttccaat ctctagtgac aaataagccg attgatacaa ggaatatatc ggtccaatca
    15961 ataattgtct gcttatccat tcactctgat caacaggaga caacttcatc agacgatacc
    16021 ttactagtaa agccaataga gtaaccgcta ggatgaatag tagaacatac caatgaaaga
    16081 cttgtatcag ctgaataccc caatgctctt gcatgaccat tcctgaacca ataagctgag
    16141 gcaaaacgaa caaccgcata ctaaacaaga tacctagcaa aaaggtgagc aatagcatcg
    16201 gatatgcaag cagctttttc aaatcattgc gaaatccttc aacaagccga aactgtttgg
    16261 caatttccaa caaagttgtc ggcagattcc catgacgttc agctaactcg atctgtacca
    16321 gttgctctgc tccatatcct atttgcgaaa gtacctcact aaacgcttgc ccttccttta
    16381 gctgatgttg gatatttgcc aaaataacag gtggaaacga acggatcgtg agtaataaat
    16441 aaagggattc ttgcagacta aaaccagata aaagcagttc tcccatggtt tgtgcaaact
    16501 ggttttgctg gcttcttgtt aatcctttag aatagttctg actgccaaat attttcctga
    16561 gcttcttcat aactttcctc ccatgatttt tttactgatt cttcctccat aaattgataa
    16621 ctccataaaa cagaaggatg gtgattggca tcttccaaaa gttcttgata aatgacacca
    16681 gctaaacatt cagatagctc ttcttttttc tttgtcagtt catggattct gaccaatgta
    16741 ccttcaagat tgcgtgcatg tacggttgca aaaacccggt gccctgttag tgctgctctt
    16801 accgcagcat gtgcagttga ttcatctctt atctcgccaa tgattagcaa gtctggacga
    16861 tgccgtaatg cgagtttcaa aagtgtatca taagtctgtg ctattttcgg attgatctgt
    16921 agctgcagaa aaaacggttc ttctatctct acaggatctt cgatagtaat cacttgcaat
    16981 ttttcttcca aagccaaccg atacattaga gaagtcttgc ctgatccgac tggtccgctg
    17041 aaaaggtata atccacgtct gacggttttc tgtaaaatag ttgcaaaatc tcgagaacta
    17101 aagcaacgat ggggtgtttc aaaagtaccg tataatagcc gaatcactaa actctctcgt
    17161 tgaaggtaat ctcctacaga agacaaccgc agacgaactt cctgtttttc taaagaataa
    17221 gtaaatgcac ctaactgagc ttttcttttt tctccgatat ccatctgtcc caaatactta
    17281 aatcttgaga tcagttgctg tccatctgcc gaagagactt tcttccagaa tgttcgtttt
    17341 tttccatagc gaaaatagat gtaaaatact tctgaagctg gcaatatata tacgtcttgg
    17401 gcatgtttgg caaaactttc acttatcatt tgatccgata atcgttttat ttccattatt
    17461 ctcccctcca caaaaaacat acgtcttttt gatctttttg tttcaaataa aatgaattta
    17521 caaaaaaata ttggcaaaag ccgaatttat gttatactta taaaaattta tcttgggagg
    17581 cactccattg gaaacaacat taaggaaata tatcgaagta gcaagtaaaa aaaagcctgc
    17641 tgacctagtg atcaagcatg ctaagatcgt caacgtcttt acgaaagaaa tcatggaaaa
    17701 agatgtagca atctgcgaag gaatgatcgt aggaatcggt gaatacgaag gaaaaagcgt
    17761 acataatgct aatgggcaat atctagtgcc aggattcatc gatggccatg tgcatatcga
    17821 aagctcactt ttatcaccaa gcgaattttc aaaagtatcc ttgcttcatg gagttactac
    17881 tgtagtgaca gatccccatg aaataggaaa cattgctggg agtacaggtc ttgagtttat
    17941 gatagaagat gcaagacaaa cactcatgaa tatctttttt atgctacctt cttgtgtacc
    18001 tgtaacccct tttgagacca atggtgcaaa tttaaatgca aaaaaattag cccctttttt
    18061 acaaaaaccc gaagttttag gcttagctga agtcatgaac tatcaagcag tagcaaataa
    18121 tgaaacagac atcctagaaa agatccaatt gatgcaccag cataagaaaa aaattgacgg
    18181 gcatgcagct ggtatcggca tggaagagtt aaatgtttat cctgcagcag gtattcggac
    18241 ggatcatgaa gctacaactg caaaagaagc taaagaacgt ttggatcttg gtatgtattt
    18301 aatggttcga gaagggaccg ttgcaaaaga tctggcatcg ctactaccgg cgatcacacc
    18361 tgaaaattca agaagatgct tctttgtcac cgatgataaa ctgatcaatg atttagtaaa
    18421 agaaggatca atcgatcata tcgtccgtca agctattgca cttggaatcg atccattaca
    18481 agccattcag atggcaacat tgaatgcagc agaatgtttt gatttgaaaa cactaggtgc
    18541 gattgcccct gggtatcaag ctgatttctt cttaacagac gatcttacca agctgccaat
    18601 tcatgaagtt ttctataagg gaacattagt tgttcaatct gggcaactcc aagaccattt
    18661 atttgctgat cattcaaatt catatacgtg ttgtctgcca aaactaaacg ctcaaccatt
    18721 aaatccacat tcgttagagc tgcttctttc ttcttcacaa gcccatgtga tcgaaattat
    18781 cccgaatagt ctagtgacta aagatttggt agaaaaagtg gaccgacaag gcgaacgatt
    18841 cattccttca atcgaaaaag accaattaaa gattgcggtg atcgagcgtc accatcaaac
    18901 gggaaacatt ggattaggca tagtgaaagg atttcagctg aaacaaggag cgatagcgac
    18961 aacgattgcc catgactcgc ataatctagt tgtcgttggg acaaacgacg aggatatgct
    19021 gtatgctgct aatcttttga ttcaaaaagg tggcgggatg accgttgtca acaaacagca
    19081 ggaaatagct tgtgtttctc ttcctatagg cggaatcatg acacaggaac ctttttcgat
    19141 cgtctatgaa cagttgaatg aattaataac taaagcacat caactaggag cagctaaaac
    19201 ctttgatcct tttttgacac tttctttctt aacattatct gtcatacccg aattgaagat
    19261 caccgacaaa ggattatttt cattttctcg cttccaattg attccacctt gtggaaaaac
    19321 cgatgaaata aatacatttt aaatcaaata aatcccagaa aatacattac atctgtaatg
    19381 ttcattttct gggatttatt tatggatatt aatgttttta tgaaagcaga aagttagttt
    19441 cacagacttt tctttttggt cttatcttga aattgagctt ctactcgata aatacgttga
    19501 ccttttgaag aaaatttttc ttcatactcc gtcataatat tgccttcaaa ttgactttga
    19561 tgtaaatcta accatacctg tttgaggatc atgccatatt gagaaaaact tgccagagaa
    19621 tactcaaaca atccttgatt atctgttttg aaatggattt ctccatttgg acgaagaatc
    19681 tgttcatcta cagcaagaaa agtcttatat gtcaatcgcc gtttttcatg acgttttttt
    19741 ggccatggat cggaaaaatt cagatagatt tgatctactt cgctgtcagc gaaatactct
    19801 gtcaacgctg atccatccac atgtagaagt ttcaggttag gaagcggttg ttcgatcaac
    19861 ttgtctagtg caatcgaaac aacactgacc tgcatttcta ctccaatata attgatttca
    19921 ggatgggctt ttgccattcc agtgatgaac tggcctttcc ccataccaat ctcaatgtgg
    19981 attggatgat cgttttcaaa caattcattc cattttccct tccacaatgt tggatcacta
    20041 attacaaaat tcgggtgagc cgctaacatt tccgctgctc ccggacgatt tcggacacgc
    20101 attatttatc tccattcctt tcctctaatt aaaaaggtct ggccaaacct cagccactga
    20161 aaattcgttt taattgtaaa atttcttcgt tcatccgtcg atgatcttca cgtttgcaat
    20221 atctggtgat ttcttggagc aaattcatgg ccgcatacca atgaaatttt tcaagcattt
    20281 cgtcagtcgg gcgtatgcca taactgatca gccactgatt ccagctagac agtggcacat
    20341 aatttcctaa aatcgttgca atatccaccg cagggtctgc aaacatgatt gaatcccaat
    20401 cgactagata taaataattt ttacatacta accaattacg atggtttaca tctccgtgta
    20461 ctactgataa gttttcctta tctattgacg ggatctgctc acgaagaaaa cgatggacaa
    20521 tttgcagaaa ttgatttttc tggagttctt ccggtaattc tttttcatat ttttgcagca
    20581 tcttctccgg cgtcatgaca cgaccaccga tacgggtcag catggtttta agagaatttg
    20641 aatgatgtaa atggtacaaa acatcaatca cgtcgtttct ttgaccaatc tcattaggag
    20701 tcagcaagcg accttccagc cactcttgtg ctgtcaaagt atcgccattc cccgttcgtt
    20761 ttgtccaaac aagttttggt gtaattcctt cccttgaaag agcagccaac atcggagtcg
    20821 tgtttcgttt aataaagact ttgtcctcat cacgcatgcc tatgtatgtt tttcctgtgt
    20881 cgcctttgat gggacgtaac cgccactctt ggtctaactg aaactccatc atgcattctc
    20941 ccgtcctctt actctttgtt ttgcagtact tattattcta gtcgcctgaa cactacacgt
    21001 caagcacata tacgttcttt ttcatttaat tgccattcaa aaaaccagct tttctcaaaa
    21061 aaaatagaac gaacagcatt cttactgtca acgctctatt tttcattctc atttttaccc
    21121 tacacagctt tgttttcagt ctgacatctt cttcaaacga gttggtacat atagtttaac
    21181 aaagagcata gtaacaatca aataagccgc tagcggaacc catcgctgct gcccatctaa
    21241 tacgactgca gcaatcaaac caaaaataac cgacgcgata attaataccc atgtgagtag
    21301 atactgaatc gcctgcttct tctgattctt atggattgga taaagctgaa cgagaaccat
    21361 atagcggaac tggttcgcta aaggtaacag ttggaaaccg atcaaatata aaaataagaa
    21421 accgataatc agactaaaat aaaagtcgac tactccggca atcaataacc cacctataat
    21481 tactaatcgg aaataaagac cactaaaatc agaacctctt gccaatcttc ttgcaaacaa
    21541 gtaaagataa gtgttttttg attctctttt aatatgagtc aatataggat ccaaatagcg
    21601 tcttcttttg atctttgccg taatttcagg aacatctgtg aataaattga taaattgata
    21661 gatctgatgc aacctatgtt cttctacatg caccattttc tcccaatcca atcctttttc
    21721 ttgttgcttc cacaaaaacc agtaatatag acttgcctgt actaatgcac ctgctattcc
    21781 tgcccatatt agccaataaa atgatagaaa taaaaagatc agagaagaaa aaaaccagcc
    21841 taatttccaa taacgaataa cttgttgatc tagtcgaaac aattctgctc tcttgatttg
    21901 cagatgactg aatttcaaac tccataatat gatcagatag acgaaaaaca tcgaaaagga
    21961 ttgctccgta ctaaccacca ctaatggcat ggccaatccg catgccaata tcaaaaagat
    22021 caacggaaaa atgcaagaat agcgaaaagc tcgttccaaa taggtttgca tttctttttc
    22081 tttaggaagt aaaaaaacaa tatctgctgc ctctgcaaaa gtagccatct tacccacgaa
    22141 taaacagagc atccaaaata cagagattat gagccctcca attgcaaacg gtggctgcag
    22201 ggttttcaac caattagaat aatataatcc cacaccgcca atcaagaaaa gacaaactaa
    22261 aacaaaatga tcgttcaata cataacgcat atatttcatc attttctttt gatgtcttgc
    22321 taaacgtttt tgaaaaaaac tactcatgtg cctgctcctt tgttaaagca atatagattt
    22381 cgtccaaaga agaatccggc agctgaaatt cttttctcaa catctcaaga gaaccgtctg
    22441 ctcgtatctc tccgttatgt aagaccacaa aacgatcaca atatttttct gcagtagcta
    22501 aaatatgcgt agacatcaaa ataccggctc cttctttttt catttctacc atcaattcaa
    22561 gcaatgcatt gatagctagt ggatccaagc ctaaaaatgg ttcgtcgatg atatataaag
    22621 aaggctcgat caaaaaggcg cataagatca tcactttctg tttcattccc tttgaaaaat
    22681 tagctgggaa ccagtcaaga cgattagata accgaaaagt ctccaacaac ttttctgctc
    22741 ttttcattgc tgtatccata ggtatatcgt aggccatcgc agtgacttcg atatgttctc
    22801 tcaatgtcaa ttcttcatat aaagaaggag tctctggaat atacccaatt tgtttgcgat
    22861 attcttccgt atttttcttt aaggatagat gattgatttc tatcgttcct tttgaagctt
    22921 ccagcagacc gataatgtgc ttgatggtcg tacttttccc tgctccgttc agtccaatca
    22981 accctactat ctctccaggc tgtacatcga aactgatatc ttttaacaca gggagatgtc
    23041 cgtacccccc tgtgacatga tcgataatta aactcattct ttttcctccg ccatttattt
    23101 ttatttatta taccacgaag aaaatgacgg tattttgttt ttttccttac aatagcttta
    23161 acttatggta aagttaagct gaagtattta ttctttatag aaaggatgtt ttatatggaa
    23221 aactgtattt tttgcaaaat catcaatcaa gaaatcccaa gctataaaat atatgaagac
    23281 gacaaggtct atgctttttt agacatctct caagcaacga aaggacacac attagttgtg
    23341 cctaaacgtc atgtagcaga tatttttgaa tatgatcccg aacttgcagg agaggttttt
    23401 tcgcgtgtgc ctaagattgc tcaagcattg gaaaaagctt ttccagaaat gaaaggtctt
    23461 aatatcttga ataacaaccg tgaactagcc tatcaatctg tttttcattc acatatccat
    23521 ttagtccctc gctacagcaa agaggatgat ttttctattc actttgtcaa tcatcaagat
    23581 agttatggat cggaagaact aaaagctatt caagaaacaa tcgtaaagca ggtgtcctgc
    23641 gatgattaaa aactttttca aaggactgtt atttggagct gctgctggca ctgtcggagg
    23701 tctgttattg gcgcccagga gcgggaatga aacaagaaag aaactgatcg aagaattgga
    23761 agaagcaaca actctgacaa atgatttaaa taacagcctg aatcatttta aaagcgcatt
    23821 gatcgaaaca aaagaaacag ctgaatctgt gattcctgct ttccaagaag caatcaaaaa
    23881 agatatcgaa gattttaaat tccaagccga accacgtatc acacaaatca atgaacaagt
    23941 agaaaaacta aaagctgatc tgccagagta tcctgaacaa atataataat gattattttc
    24001 tcaacagatt taactatata agtgagaaaa gtttgcgtat ttcctaggaa tgtgatgaaa
    24061 ttttgaattt tgtttgaaac taccacgaca acgtttttaa aggaaatggg atatggtata
    24121 gttaatatat aaaaataata actaattaca ggagtgcaaa ttttaatgaa aaaaaaatca
    24181 attattttgg cagctactag tgcgttggct gttttaactc tagcagcttg ttcaggcgat
    24241 actaataaag atatcgcaac gatgaaaggc ggaacgatca ctgtatctga tttctatgat
    24301 gaagcaaaac ttaaatcatc gaaccaatcc ttagtacaac ggatgatcat ttataaagta
    24361 ttcaataaca aatatggcga taaagtaacc gataagcaag tggatgcaga atacgacaaa
    24421 caagcgaaat cattaggtga tacttttgaa tcacaattag aagctgctgg atatacgaaa
    24481 gatacttata aagaatatat tcgtaacaat ttagcttttg aagctggctt gaaagctcac
    24541 gtagatatta cagatgacga tttgaaaact gcttggaaat ctttccatcc agaagtagaa
    24601 gcacaaatca tcaaattatc aagtgaagat gaagcaaaag atgtgaaaaa atcagcggat
    24661 gatggagatg atttctctaa attagccaaa gacaaatcaa ctgatacaga gacaaaagaa
    24721 gatggcggta aagttaaatt cgactcaacg acaacaacta ttcctgcaga agtaaaagaa
    24781 gcagctttca aattgaaaga cggtgagatc tccgatgtta ttactacaac aaatcctact
    24841 tcttatgcga cagaatacta cgttgtcaaa atggtgaaaa accaaaataa aggcaatgac
    24901 atggataaat acaaagatca actaaaagat atcgctactg aaactaaact aagtgacaat
    24961 gcattcacaa ctaaagtaat cggtgaagaa ttaaaagatg caaatgtaaa aatcaaagat
    25021 gatgcctttg aaaatgtgtt gagtgcattc acaacaactt catcaagtac gaaagactca
    25081 agtgaaacta cagcttcaac aaagagttct gacacaaaat caactgattc aacaaaagaa
    25141 tcttcaactg aagaaacaac tgattcatca aaataaaaga aaacaaaaaa agcgaagact
    25201 tatgtctcgc tttttttgtt ttgaatgaaa aaaataaatt tttgctataa atcttttggt
    25261 acatagaaac ttcggttgtc cataccgaaa atccgatcgg tgtattttcc tggttctgtt
    25321 tgacgaatcg aagtcaacat catctgcatc gaagcatcga tattgtcgat ttgatgtaat
    25381 atttctgctt ccattattct tggacgtaca ggtgagccat attctaataa gccatggtgt
    25441 gctaaaacca tatgacgtaa tacgagaaca tcttcgtcag cttcgctgat tttcaacgca
    25501 agacaggctt tagttatttc ttcgtcaatc aataccagat gaccgatcaa atttcctgca
    25561 agcgtatatt ctgtcgacat aggaccggaa agttcaatca cctttcctaa atcatgcaaa
    25621 atgataccag catacaacaa tgctcgattc agctcaggat attcatcggc gattgatttc
    25681 cctaagtgaa gcattgtcaa agtatgataa gctaatccac tggcaaatgc atgatggttt
    25741 ctttttgctg ccggaaactc aaaaaattgt ttttgatatt tattaagtag atagcgaaca
    25801 atacgattcc aatgcgcatt ggtgatctca aacaacaatt gattgatttc ttcttccatt
    25861 tcttctttct tcaaaggggc tctttccatg tataaactag gctctcctgg ctcatctacc
    25921 cttgttaagc gcatatgaag aatctttact tgagggtttc cttgataaac ctctctttta
    25981 ccgtttagaa aaacgactgt gccggctgta aatttattga tttcctcctc cgaagcatcc
    26041 cagtatttac catcgatagt acctgaagta tcttggaaag taaaagcaat aaattttttt
    26101 ccgtttttag cgattcttac atctgcactt ttgatcagga caaattgttc aaactgctca
    26161 tcgattacta aatcacgaat ttttttcatt atttgctcct tctaattgtt ttacgatttg
    26221 gttcatctca tggtaatagc ttaccatttc tttatcagaa gaaaaacaaa ttatttgata
    26281 cttttctcca aatttagcta aaagttcagc taattgatac tttcgctgat gatcataatg
    26341 aagccagcca tcatcaatga tgatcgggca tatcgaacgg ttagcttcta atgagaggta
    26401 agcaaatctc atcgccataa tcagctgatc tttcgtacct gtagataact catagatggg
    26461 aaattctgta tatccgtctg ttaaagtcag tgtatcattt ttcagtaata tcttctgata
    26521 atttcctttt gttaaaatac ctaaataatc acctgctgct cttaacaact ggggtaattg
    26581 tctttcactc atctctgttg aaagatcacc taagaatgtc ccggcgattt caagtgctcc
    26641 ccattcctcc agtaattcct gtaattttgc tttttgccgg ctagcttgtt gataaagttc
    26701 gtccaatgtg ccatctcttt gcaactcttc tacttgaagt tggagtcgtt gccgattttc
    26761 acttgcttct ttttcttctt gctggagttg ggattgcttt tctttcagtt ggcgcagtct
    26821 tacagataat ttctcttttg tcagctcttc aggaaatagt ggagtaagca tttccgataa
    26881 ttcattgtat cttttcatct gctgttcggc ttgtttggct ttttgtaaaa agacaggtac
    26941 ttccgaagga taagataagc ctgcttgagt caattgttcg ctgtattctt gtatccatac
    27001 tttttgtttc tttttcaatt gattgatttc ttgcttcaat aaagtatttt gctgatagct
    27061 tctggcaaat ttcatctgtt ccatctcttg tataaaacgg gaaagcaata ccattttttc
    27121 tgaaattgtc ttatcttgta tcggtaacca ttctttaaca aactcaaatt tttggtcgat
    27181 ttgtcgtagc ttttcttgta tttcctgttc tcttttatgt ttccgctcaa atacgccata
    27241 taatttctga taatctatga cttcctgtcc atattctttc atgagtttga gcgtatccat
    27301 ctttcctaag tgccgatgat ccgcaaaacg atatacttct tgctgaagaa tctgtttttc
    27361 ttctccagtt ctcttttgtt tttcttctgc ttcagctaat tgagctaagg ctagatcaag
    27421 ttgtcctagt ttttcttgcc acattccttt tatctcagag aaatcatttt tagttttaat
    27481 ttttggttgc cttttgtaga agaaatagcc aatcatgcct gcaccaatta gtaaaaggat
    27541 cactttcaag ggcattggta caaatatacc tgcaatgatt attgctccgc cagccatcag
    27601 catatacggt ttagatgaag agactggtgc tatctctgac tgattttgta ataatgctgg
    27661 ataggtctct tccaagtcat tcacttctgc ttcaatcatc tcttgctgtt ctttagctat
    27721 ttggagacgc acagaaatct ggctttctag atcagcaatc cttctttctt cagccagaag
    27781 atgcttccat tcttcttcat ataaaaattc ttccggtaac ggatttttct gccaatgcca
    27841 cctttgttcc aaaatcttca attctgaatt tgtttctcta gtctcaattt gtaatctttg
    27901 ccattcttcg tttaattgtt ccgctgtaaa ttgaagttgc tggagatctt ttagttcctg
    27961 ctcctgctct aaataaaaat aatatctggc tgactgctga tccatcccgc tatgcttttc
    28021 cagtgttgct tctaattctt ccaaacgctc atttgtttgc tgataccgtt tggagaaaga
    28081 ctccagatct aaaagaagtt gctcatcagg aagacctgta gtgctgtttt ttttgagaga
    28141 ttgccattct tcatataaag aaaaattaag ttcttgctgt ctggctaatg aaaattgttt
    28201 gttcgcctct tttaattgtg ccaataactg ctgctgttgc gactcgtatt cctgttcttg
    28261 ttggatcaac ttgccaaaat aagcttcctg ttcttgtttt tgatgaattt tttcttgcag
    28321 ctctttccat tcatttaatt tttgattgat tagtagtttt tggccttttt tcttgaataa
    28381 ttgttgtgct ttctgataat attcttgccg cttttggaat acttgagaac tgcccgtgat
    28441 gcctaaagaa atcagcgcat catgtagctc tttttctctc aaatgatcta gttccatcaa
    28501 ctgctcttgc tggaaagtaa atacctgctg gaataactct ttcgtcaaag gtgcgatcag
    28561 tttttctagt aactcatcac ttcccacttg atcccccagc cacaccttcg attttccgcg
    28621 attttgctgc ttgtagcgct cgacagcaaa ttcaccatat acaggatgtt taagccatat
    28681 tttgccgcca taggctgagc cgtcttttgg tgtataatcc ttttttttac ggccttttga
    28741 agggaaacca aataatattg cttgaataaa ttgataaatc gttgattttc ctgcttcgtt
    28801 tcccccatag atcagctgat tgcctgaata aaagtcaaaa cttttttgac gccattttcc
    28861 aaaagcagca atctcgatcc ttaaaatctt cattcctgat cctcctcaaa taaaaaatct
    28921 tgattaagca gctgcttggc tttttcaagt gtttcttctt gatattccgg cagttcgctc
    28981 aatattctag ccgcttcttg atgactatat acttcattta aaatttgttg gaaatcttga
    29041 ggtgtctgat agtattgaaa taactgttcc atcaatttag cagaagcagc aagtgggatc
    29101 ttcgtataaa aagtattctc tatgagagaa atacgccaaa taaagaaatc atttatccta
    29161 tctgagaatt cctctaacaa atagtctaat agctctcctg attgtatccg ttccaaaaca
    29221 tctgtattga gatggtcata atcttttaat tttatctcaa ttaaactaaa tccatcctct
    29281 acttttgaaa gctgctgacg aatgtgaaca atgacctctt ttgtcgttct tgctttcctc
    29341 aatgatattt cctttgtctt ccaatagacc tctgctactt taatcggatg tatttggcaa
    29401 cttcctttac ttaattcaac tagtagaata gatgtagaag cttgttcctt tttagtatga
    29461 ccttgaggcg cacctggata aacaattgtt tgcttttcat ttaagaccgt tggcacatgg
    29521 atatgcccta aagcccaata atcgtatccc ttttcttgca tctttgaggg ttggaaagga
    29581 gcatagtttc ctttttgaga gacgccaggt tccccgtggt acaacccaat atggtaatct
    29641 gtgagttctc tagaaggaaa ttccatcact ttgtcttttt gaatccacgg ttgacgataa
    29701 ctaaatccac tgagagagac agtttctcca ctttttatcg ttattttttt tgtctctacc
    29761 atttcggatg taaataaatg gacattttta ggaaaggaga accaataccg ctctttttga
    29821 tagaaatcat gatttccgaa tattataaat acaggtatat ggttcttttc taaccgtttc
    29881 atctgttctg aaaaatgttt ctgtattttt aatgaaggac gattttgatg aaaattatct
    29941 cctgcaagta aaacaaaatc aacgttattt attattgcct gttcaatgat atttgataaa
    30001 acggtcagat tcgtcttcaa tagtttttcc tgtacagttt tatcaagagt tgtcagtcct
    30061 tcaaatgacc gatccatatg taaatctgcc gcatggataa atttcaccat taaaaatttc
    30121 ctcctttttt tcttttatca taccattttc aatgtttttt ttcatcaata accgaaaatt
    30181 tgttcgtatt atgcaaagaa attacgataa atggccgact tcaaaaaata agtttattcg
    30241 aaaaaaagat tgtgacatga tttttgtcac aatcctaaat atctatgtta tattcgcttc
    30301 gtttgacact gaagaagtac tatttgtact ggaatttatg ctccattttt ccgtatttag
    30361 aattctatgt tctatactac cttaattagt ttatatggta agaattcttt caaatgcttg
    30421 agatactccg tctttatcat ttgaaacagt gacataatca gcttttttca tgatttcttg
    30481 atgactattt cccattgcaa aactaatatc tgcactttca aacatagata agtcatttaa
    30541 actatctccg atcgcgattc tcaaaggttt ctttaaattg tagattgatt ccaaacgatc
    30601 aaacaccaca cctttagata cattttgagg tacgatttca atatttacag gtgccgattc
    30661 agtaacagac acatttagca tctttttcaa atggttaaat atattagctt tcaattgacg
    30721 attaggaata aaaataaaaa atttgtaaat gctgatagca tccttttcta taatttcctc
    30781 aatattatta taagggattg aacaaataga tttttcatac tcaagctcat agtttagctc
    30841 tttgatagaa aattcactcc ctacctcgtg aaaaattttt ttcattttat ttttataatt
    30901 ctgagattca taacttccga gatttgtata tattttggtt ggaaatttcg ataggacaga
    30961 atcaattttt tttacatcat ttttatcgaa ggaagactca aatatctttt tttctccatc
    31021 aaatccaaca gaaccattta accctactgc tggtatcctt aaaccagatt ttttgagaat
    31081 attattaatg tcttctactt ctcttccaga acaaatgaat ggaaaacagt cttccggtaa
    31141 atgtttcatt gttcttgcat ttttttgact aatgctctgg tcagaattca aaagagtacc
    31201 gtctaaatct ataccaatta gtttcatttc atccatgtct cctatacgtt gattataaat
    31261 tcactgctta ttgtaagtct aaatttctca caaaacatat actaattata cctaatttaa
    31321 gtaagtacaa acatcatttt aagaatagga aaggacattt atatactatt ctctgtattt
    31381 ttgatttata
//
LOCUS       DACCKU010000026        30258 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.026, whole genome shotgun sequence.
ACCESSION   DACCKU010000026 DACCKU010000000
VERSION     DACCKU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 30258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.026"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            135..1454
                     /locus_tag="IVN13_001113"
     CDS             135..1454
                     /locus_tag="IVN13_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAP7011614.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            1895..3535
                     /locus_tag="IVN13_001114"
     CDS             1895..3535
                     /locus_tag="IVN13_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP7011615.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAVFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            3689..5011
                     /locus_tag="IVN13_001115"
     CDS             3689..5011
                     /locus_tag="IVN13_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP7011616.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(5073..5504)
                     /locus_tag="IVN13_001116"
     CDS             complement(5073..5504)
                     /locus_tag="IVN13_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7011617.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(5568..6404)
                     /locus_tag="IVN13_001117"
     CDS             complement(5568..6404)
                     /locus_tag="IVN13_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAP7011618.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQAIVIVVLLINLYNAFVILQTLEGSGFKQVKITEKSF
                     FK"
     gene            complement(6594..6896)
                     /locus_tag="IVN13_001118"
     CDS             complement(6594..6896)
                     /locus_tag="IVN13_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP7011619.1"
                     /translation="MTEHNHSHEHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEE"
     gene            complement(6922..7353)
                     /gene="ruvX"
                     /locus_tag="IVN13_001119"
     CDS             complement(6922..7353)
                     /gene="ruvX"
                     /locus_tag="IVN13_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP7011620.1"
                     /translation="MRVMGLDVGSKTVGVAVSDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(7350..7616)
                     /locus_tag="IVN13_001120"
     CDS             complement(7350..7616)
                     /locus_tag="IVN13_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP7011621.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7870..8595)
                     /locus_tag="IVN13_001121"
     CDS             complement(7870..8595)
                     /locus_tag="IVN13_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP7011622.1"
                     /translation="MSKKTTKAIVGIVAAVIAGALGISTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8961..9031)
                     /locus_tag="IVN13_001122"
     tRNA            complement(8961..9031)
                     /locus_tag="IVN13_001122"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8997..8999),aa:Gly,seq:ccc)
     gene            9148..9900
                     /locus_tag="IVN13_001123"
     CDS             9148..9900
                     /locus_tag="IVN13_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP7011623.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9916..10395
                     /locus_tag="IVN13_001124"
     CDS             9916..10395
                     /locus_tag="IVN13_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP7011624.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(10646..12226)
                     /locus_tag="IVN13_001125"
     CDS             complement(10646..12226)
                     /locus_tag="IVN13_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP7011625.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(12219..12962)
                     /locus_tag="IVN13_001126"
     CDS             complement(12219..12962)
                     /locus_tag="IVN13_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011626.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(13138..13914)
                     /locus_tag="IVN13_001127"
     CDS             complement(13138..13914)
                     /locus_tag="IVN13_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011627.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(14162..14998)
                     /locus_tag="IVN13_001128"
     CDS             complement(14162..14998)
                     /locus_tag="IVN13_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP7011628.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(15009..15938)
                     /locus_tag="IVN13_001129"
     CDS             complement(15009..15938)
                     /locus_tag="IVN13_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP7011629.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            16130..16483
                     /locus_tag="IVN13_001130"
     CDS             16130..16483
                     /locus_tag="IVN13_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7011630.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(16557..17213)
                     /locus_tag="IVN13_001131"
     CDS             complement(16557..17213)
                     /locus_tag="IVN13_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7011631.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            17439..19370
                     /locus_tag="IVN13_001132"
     CDS             17439..19370
                     /locus_tag="IVN13_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7011632.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEIAELLAQDEPESAPAPTNKSDYYQSKEQ
                     QKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQDQ
                     ETKLTEWEELSLELEEYEN"
     gene            complement(19456..20679)
                     /locus_tag="IVN13_001133"
     CDS             complement(19456..20679)
                     /locus_tag="IVN13_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP7011633.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(20673..22571)
                     /locus_tag="IVN13_001134"
     CDS             complement(20673..22571)
                     /locus_tag="IVN13_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP7011634.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(22565..23683)
                     /locus_tag="IVN13_001135"
     CDS             complement(22565..23683)
                     /locus_tag="IVN13_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7011635.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(23766..24599)
                     /locus_tag="IVN13_001136"
     CDS             complement(23766..24599)
                     /locus_tag="IVN13_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7011636.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVSATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(24592..25509)
                     /locus_tag="IVN13_001137"
     CDS             complement(24592..25509)
                     /locus_tag="IVN13_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011637.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(25736..26860)
                     /locus_tag="IVN13_001138"
     CDS             complement(25736..26860)
                     /locus_tag="IVN13_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7011638.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTTTVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            27043..27951
                     /locus_tag="IVN13_001139"
     CDS             27043..27951
                     /locus_tag="IVN13_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7011639.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            28092..29705
                     /locus_tag="IVN13_001140"
     CDS             28092..29705
                     /locus_tag="IVN13_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP7011640.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         9424 a         5947 c         5172 g         9715 t
ORIGIN      
        1 attaaaacca tgtgaaactg cctcacggca gcttacaatt actttttcat cttcacccgt
       61 taagtgatga aaaaagaacc cctttctgct atggttaaag tgactaaacc aaccaaaaga
      121 aaggagttct tttcatgact agcttacaca aaaaccaagt aaaattcaat tcaaatatca
      181 ctatttctca tacaggtggt caattatcga gtgattcggg tctcgtccta gtgaaagagc
      241 tgatgaacat ctttggtttt tctgaactgg ctaagcaaca cattcacatt gaagacgaac
      301 gagcgtattt tactcatgac aacttatcca tacttgagca gttcattatg caattaattg
      361 ctggttactc agctgattct gcagctaatc tcctgagaca agatcctgtg tttaaagctg
      421 ttttagatag gaaagaactc gcttctcaat cttcactttc ccgattttta gatcgactat
      481 ctgaggagaa tatccatgaa cttcaagctt tgaaccaaga acttatcgat aaagcacgcc
      541 tcatccgtaa tgatacggaa ttaatcattg atttagattc gacccattca gatacatttg
      601 gtcaccaaga acaaacgaat tataatacac actaccaaac ctatggttac catccattgg
      661 tagcttttga cggattgact ggtgacttct taaaagctga actacgttca ggaaatcaat
      721 atacatcaaa aggcgtaaag gagtttctca cacctttatt agagcactat aatcactctc
      781 taccaaacac tgacatcttg gttcgtggag acagcgggtt cgctacacct ggtgtgtatg
      841 attcgtgtga atcaaaaaag agtcattatg ttattcgact gaagaataat cgtagactag
      901 gtcaaatagc tgagaaatca gtactttatg gcgataatca aaagtgggaa gaacgagaag
      961 ttcagtactt ctcgaccact tatcaagcac aatcctggtc acaaagtcgt cgcgtgtgta
     1021 tacgctcaac acgtgaagcg ggcgaactac tctttcgaca tgaatttatc gtgacgaatc
     1081 tatcagaaaa tgtttctcct gataccatct tttctatcta tgccaaacgt ggcacaatgg
     1141 agaacttcat taaagaagcg aaagctggct tttactttga caaaacagac agtcctcgct
     1201 ttttggagaa tcatgtccga atgatgctca gcttgatagc ttacaactta gtcaacttct
     1261 taagaacgat tggctttgaa gaagtccaaa agggaatgac cattcattct attcgattga
     1321 agtttctgaa agttgctggg aaattagtcc aaacgggtag acgagtctat ctcaaattat
     1381 ctagttatca tgtgtatcag aatgaattct acagggtctt tgctcgcctg aggcgagcca
     1441 gtcaatggat ctaatccaac aatctaacga tttttttact gggaagttat ccagggagaa
     1501 gtatgctcaa aatgccttac acccaaaatt tattcctcaa tatttttgaa cagtgaatgt
     1561 ttgaagtaac tttttagtca aaatcagttg atagggatat tagtgataca tatttcaata
     1621 aaatgtgcca aagaattaaa gctatgaatt attcaggatt ctatttatct atctaacata
     1681 tcctttattc ttctactttt ctttctttag catataagag atacgcatag taatcaagca
     1741 aggattgttg atataaaaag aacaaatacc gctattttga tttcttttga ctctattact
     1801 catataaata aaatttatta tacctgctct ttttgactat atcctcatct gactcctata
     1861 cttcgtttgt attaaaactt aggaggaatc attcatgctt acagggctaa acttattaaa
     1921 taatcctttt ctaaataaag gaacagcttt tacaaaagaa gaacgggcaa aatacggaat
     1981 cactggtatg cttccaagta ctgtccaaac attggaacaa caatctgtcc aagcttacgg
     2041 tcaatactta agcaaacaga ccgatttgga aaaacgtatc ttcttaatga atctattcaa
     2101 tacgaaccgg acattgtttt ataaattgat gggcgaacat ttagttgaat tcatgcctgt
     2161 cgtttatgat cctgtcgttg cggattcaat cgaacaatac aacgaaattt tcttgaaacc
     2221 acaagatgct gtatttcttt caatcgacga accagaaaat atcaaagcaa gtttgaaaaa
     2281 cgcagcagat ggacgcgaca ttcgtttgat cgttgtgaca gatgcagaag gaatcttagg
     2341 tatgggtgac tggggtgtga acggtgttga catcgctatc ggtaaattga tggtctacac
     2401 tgccgcggct ggtatcaatc ctgcacaagt cttgcctgta tcaatcgacg ctggtactaa
     2461 taacgaagaa ttattgaata atccattata cttagggaat cgtcatgctc gtgtagaagg
     2521 cgaaacttac tacgaattca ttgatcaatt cgttcaatca gctactgaat tattcccaga
     2581 attgttatta cattgggaag attttggacg aggaaatgct gcaacgatct tagaaaaata
     2641 cgaagataaa atcactactt tcaatgatga tatccaaggc acaggaatcg ttgtactagc
     2701 tggtgtatta ggtggattga atatatcagg tgagcaattg aaagatcaaa cgatcttgac
     2761 atttggtgct ggtactgctg gtgtaggtat cgcaaacatc cttttagatg aaatgatccg
     2821 tcaaggtgta cctgaagaag aagcacgcca acatttctat caagtagata aacagggttt
     2881 attgtttgaa gatacaaaag gattgactcc tggtcaaatc ccattcgctc gtaaacgctc
     2941 agaattcact aatgctgatg aattgacaaa cttagaagca gttgtaaaag caatccatcc
     3001 aacgatcatg atcggtactt ctacccaacc tggagcattc actgaagaaa tcgtgaaaga
     3061 aatggcggct cataccccac gtccaatcat catgcctttg tctaatccaa caaaattggc
     3121 agaagcaaaa gcaaaagatt tgattgaatg gacggacgga aaagctttag ttggaacagg
     3181 tatccctgct gctgatgtgg aatataacgg tgtaacttac caaatcggtc aagcaaataa
     3241 tgcgttgatg tacccaggtt tagggcttgg tttgattgct tcaacagcta cacgagtaaa
     3301 tgctgaaatc atttctcaag ctagtcgagc attaggcgga atcgttgatg taacaaaacc
     3361 aggagcagct atcttgcctc cagtagcaaa aatcactgaa ttctctcaaa cgattgctga
     3421 aactgtcgca aaatcagttg ttgcacaaaa attgaatcgt gaagaaatca cggatatcaa
     3481 agaagctgtt gaatctgcta aatgggttcc tgaatataaa tctttagaag attaagattg
     3541 ttatagtaaa agaacataca gaaactttgt atgacgtcta aaaagaaaaa agcgactgcg
     3601 gcaaaacagc agccagccaa ctactgctct tttgccgctt ctctttggct tttttcatac
     3661 gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa acaacaaccg caactgcagt
     3721 tgaggaaaag aaaaaagaag ggttttggtc aacaaaaatt accggcgtaa gtttgcccct
     3781 ttatttgatc atggtccttg tcctgatcat tgcaatggct ttaggaaaat taccgacgaa
     3841 tatgattggc ccaatcgctg ttttggtcat tttcggtaat ttatttcatt ttatcggaac
     3901 aaaaatccct atagtcaaaa gttatttagg tggtggctcc gtttttgcga tctttgcctc
     3961 tgccgcgatt gctacatttg ggatcttacc tgagtatgta gtaaaatcca cagaaaactt
     4021 tgtcagcaat atgggctttt tagactttta tattgctgcc ttgattactg gtagcatcct
     4081 tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt tttattcccg ttgcacttat
     4141 ttcaatggtc gtctcctttt ttgccgtagg actcgtaggt atgcttatcg gaaatggttt
     4201 tgcaaattcc gttttatata tctcattgcc cgcaatggct ggtggtgttg gagccggagc
     4261 tgtaccttta tcaacgattt atgcaaatgt tttaggtacg gacgcttctt ctatcatttc
     4321 ccgcctgatt cctgcgacag caatgacaaa tgtcctagct atcattggtg cagcgttagt
     4381 tgctcgtgcg ggacaatcaa tgccaaaatt caacggtaat ggtaaattaa tggaaaaagg
     4441 cgaccttggc gatggaaaac ctagaaatgt aaaaccttcc attccacaat taggtgtcgg
     4501 tttgatggta tcattgactt tcttcatctt aggacaaatc tgtaactatt ttgttcctaa
     4561 agtccatgct tacgcattca tgatcattat cgttgttatc tgtaaaatcg caaacctatt
     4621 accagaatat tacgaagatg cagctatcat gttcaacaac ttgattgtca aaaacttgac
     4681 agcagctgtt cttgccggta tcggtattgc gttattgaat ctaaatgtat tggcttctgc
     4741 tttgacatgg caatttgttg tcttatgttt gacaagtgtt atcgcgattt cagtagtttc
     4801 aggatttgtc ggtcgtttgt ttggtttata tccaatcgaa tcaactatta ctgctggctt
     4861 atgcaacaac tcgatgggtg gtacaggaaa tgtagctgtc ttatctgcag ctaatcggat
     4921 ggaacttatt gcttttgctc aaatgggcaa tcggcttggt ggtgccatcg ttttgatcat
     4981 ctcaggtttc ttgatgcaat tactttctta gtcaaaaaaa atacctgatc atcaagtatc
     5041 ggcttgatga ccaggtattt tttgataaaa atttacagga ttttcaaaaa tcttttttgc
     5101 attttatcgg aatgataacc gatgcgttca taaaatcggt gtgcttctac tctgtattct
     5161 gcagaattaa gtcttattgc tgatattcct cgtcttttcg cttcttctac gccagtcatc
     5221 aaggcctttc cgattcctct tttttcagca tgtttagaca cagcaagtgc cataatattg
     5281 aacatcggat cgctgaaaat cgattcatag gactccgctt gtacatagcc agctgctttt
     5341 ttagaagtag gatcttcata gaccaagaaa aaattttttt ctggctcctt caacagtttc
     5401 ttcatttgtc tcaccgtaac ggttaatggt acatcatatc ctaattgttc cgcattgatt
     5461 tctcgtagtg cttctgcatc tcctaaacgc atttcccgca acatccaaat caccagccaa
     5521 tttttatttt tttaaatgaa tttttttatc acagtatact ccttttttca cttaaaaaaa
     5581 gacttttcag tgattttgac ttgcttgaat ccagatcctt ctaatgtttg caagatgacg
     5641 aaagcattgt aaagattgat caaaagaacc acgatgacaa tcgcttgaaa aataaaggtc
     5701 actttttttg gagacaaaac cctgctaaat cttgctcccc acaaacctcc aataacagca
     5761 gcggggataa cataaaacaa catagataaa tcaaagcgca taaagctact tgttaaagca
     5821 atcgttacca attttgccag ttgagaaaag aaaatcgtag atagagaata tagtgttgct
     5881 tcttttattg gcaatgcaaa catcagcatc aacaaagaaa cattgactgg tccaccacca
     5941 atacctaaaa aactagctaa aaagcccaga acccccccac atataaggta ccaagtccat
     6001 gacgtcaaat gaaattttgg ctgatgatgc ttggtgtaga agaacgcaaa aaccaaggtc
     6061 aataccgtca aaaaaatttg gatcagctgc acatgcttct cgttttcaaa taactgtaaa
     6121 aaaacttcga atacaatgtt gccgactact cctccaacaa cagctccact agatacccaa
     6181 ccgacaatct gccaattcag tgatttcccg cttgatactt gtcgcacagt cgaaacaatg
     6241 gacatcgtga ataccgcaac tgttgagtaa aaagaaatcc ctgcaacaga atgggcgccg
     6301 ataagatcaa gaatcggttt gatcaacacg cctccaccca tcccggaaat agcgccaact
     6361 gtgttcgcca gaacgatcac cacaaaataa atcagaccaa tcatcttgta tttcctttct
     6421 cttgtttata tcttttagcg attgttactc ttttctttta ttatacagta aaagaaacaa
     6481 acgaggcttc gaaagctttt ctacttttta aggaaattta gtgctaaacg aaaaaaccgg
     6541 gctctacttt tcagagagtc cagcttttct tacaccttat cgattctttt gttctattct
     6601 tcttccgcca tgaatgtatt gaatacttct tcgatcatat cccattctgc gtctgtttcg
     6661 atttgttcta actcgccttc tgttccatct tcgttttcga tgtatgcata agcttgtaat
     6721 tctacatctt catcttctga aacaccagct ggatataaaa gaacatagtt acgtccaaat
     6781 tcttcttgtc cgtcaactgt tagcaagatt tcgtataatg tttcatttcc ttggtcatcc
     6841 accaatgtga tgtgttcgtg tccttcgtgt tcgtgggaat ggttatgttc tgtcataaga
     6901 ttacctcttt tctttttatt attaaaataa ttgcttacct gagccgtcta aataattttg
     6961 taaaatcatc acagctgcta atttatcgat gacttttttt cgtttcgctc ttgaagtatt
     7021 tgcctgctct actagcatac gttctgcctg aacagtggtt agtcgttcat cttgataatc
     7081 aaccggcaag gagaatagtt ctttgatctt ctctccatat gacatggaag cttctgccct
     7141 tggtccgatg gaattgttca tattcttagg taatccaact acaaaacggt ctacttcgta
     7201 ctgcgccact aattccttca accgatcaaa accaaactct tcttctgcct cattgattcg
     7261 aatgatttct actccttgag ctgtccagcc aaagggatcg ctcacagcaa cgcctactgt
     7321 cttagaaccg acatccaagc ccattactct cataaattca ctccatgatt tgagaggtag
     7381 tctttgacaa ttacttccat gatttcatcg cgttcgtgac ggcgaatcaa atttcgtgca
     7441 tcacgataac gaggaatgta ggccgggtct ccagaaagca agtagccgac aatctgattg
     7501 attggattgt agcctttttc ttccaaagcc cgataaacaa tcgctaatgt ttcgctcact
     7561 tcttttttgc gactatcgtc aaaaccaaaa cgtacggttt catctgtaaa acccatgatt
     7621 ctacacctct ttcccgcaag gtaattccag cttaccttct acacccttat tttactcgaa
     7681 gaaagggaaa actacaaaca aatattccaa acttctgaat aaattaagaa acacgatttg
     7741 tctgattttt ccttttttct gtggcttttt caaaagccct ctttccataa agtatacgct
     7801 atatttcttt gaaagtatgt ttttacacaa ttttttcata aagttcataa aaaattcgaa
     7861 ttgctgctac taatttttcg ttttcgttgc ttgccctgtc tcataattga tggtgtagcc
     7921 atcttgaaca ttgtagataa agacattgaa ttcaattttt ttgtcttcaa tgctttctgc
     7981 ttccaactgc acacctcttg ctaccagttc atttccctcg aaatgaggag tgacacgata
     8041 gcgtacatga tgatttgttt ccttgatata tgccgcaata tcattttcgt gggcaagcat
     8101 gtacggagca ttcaacgaac gtgtccctgt cattaagttt ttaatgttat tattttgacc
     8161 agtcaactgg aaaccaatca aatggctgcg gttgtataac cattgtccgt cacttaattt
     8221 cttttgtttc caacctgtcg gatcaatata caaaggttct ctcttttcag taggaaacaa
     8281 gtcttttccc aacatcgcat tggcttgtcc tactctgttc aatttgtcta tatttgaata
     8341 gctttcccat gtccctttgg aaagactaag atcctctttt gtaaactccg gctgattccc
     8401 attgatttct atttcttgat gttttccatc atattctggc acatccgcta cttgagaagc
     8461 ttgcggtgtg gaatcaaaaa tggttgtaat ttgttggaag atatctgaat ctttttgtgt
     8521 ggtggaaata ccgagtgccc ctgcaatcac agcagcgacg atccccacga tcgccttggt
     8581 tgtttttttg ctcatttctt tactccttta ctaacagttc cctaaatctt aacatatgtt
     8641 cccctataaa taaatactgg aaagaaatag aaacagtaaa aaaataaaac cagtttttag
     8701 acggcttaag aatacaaaaa aagagacgga ttcatcgtct ctactcaatc acaaaacact
     8761 atttacaagg tatttattat cattcaaaaa atttttctta atactcaaaa ctaaacggct
     8821 tctgtacgac ctcttggtaa acagtgttca aaagcttgca tctgcggtaa taagtccaac
     8881 caagtcaaaa acataagaag cttttttgct tgactattct tattacttga tgcagggcag
     8941 ctttttcaca gcctctttta agcgggtgac gggaatcgaa cccgcgacat cagcttggga
     9001 agctgggatt ttaccactaa actacacccg cgatacaaaa acagtatacg cctctttcac
     9061 tccttcctca agttttttta ttctttacgc ttccgatatg ttttgtagta aaataaaact
     9121 aatcaatcaa ttattggaga agatgctatg acgggaaaag aattagatct gttgctggac
     9181 acttgtttac tcgctgggaa aatcatgatg gaaagcaatg cggagatgta tcgggtagaa
     9241 gatacgatga gtcgaattgc tttagcttct ggaaatttcc gtttggtcag ctatgtcact
     9301 caaaccggtc tctttattgg tcttgatcgt acctcgacca tccgaatgga acaaattacc
     9361 aaccgttcga ttaatttaga aaaagtagta aaagtaaatg atctttcacg taaatatgtt
     9421 gcttcagagc taacattaga agaactttac tgcgaactgc aagcaatcga ccgagaccgt
     9481 tctttctttc ctgtctggtt gcaaatcgtt agcgcggcag tgatcagtgg aacaattatg
     9541 gtgttatttg gcggaaagat gaccgattta cctgtgactt tagtcattgg aggattggga
     9601 tacgcagctt atctatatag tttgaaattt tttagaatca aatttttatc ggaattttta
     9661 tcttctttat tgattggcat agccgctatc tacagcgtac gttttggctt aggaagcaat
     9721 caggatctaa tcattatcgg atgtgtgatg cctttagttc ctggagttca aattacgaac
     9781 gctatccgtg atttgctagc tggacattat gtctcaggag tttctcgcgg aacagaagcg
     9841 atgatgactg ctacgatgat cggttttgcg attgctttta ttttccagct tttttattag
     9901 gaaaggaaca caagtatggg gacgatattt attcagtttt cttttagttt tttagcgact
     9961 gcggcatttg ccgttattac aaatgtacct aggcgttcat tgatttgttg tggtctttct
    10021 ggtgcttttg gctggatgat ctactggatc tgtgttcaaa taggcggatc tgctgctttt
    10081 ggttcattgc ttggttcttt aggagtagca ttcatcagcg atcttttctc taaacgtttg
    10141 aaaatgcccg taacgatttt caatattccc gggatggttc ctctagtccc tggtggttta
    10201 gcttatcaag cggttcgaaa tctcgttact gaatcctatc gagaagcggc tcattatgcg
    10261 gtccaagcaa ttatgattgc tggagcaatc gctttaggtc ttgtattatc agaagtcttt
    10321 aaccataaca tccgtaattt caaagtaaag cgtgacgcaa tcatgatgaa acgaaaaaaa
    10381 cggcttttta aataaacgaa gtgtaacaaa gaaaaggttg tgaaagagac gttcagcctt
    10441 gagcgatgac aaaatatctc acaaacattg cgacaagtaa ccgcaggagc aataagaagt
    10501 aattatgagt attaaaaaaa gttttgtaaa aatagcttct cacattttta cataaaattg
    10561 acttaatact gagaaactga cttttgaaca ccctctatct gtaaaaaagg ggctgcgaca
    10621 tgatttttgt cacggcccct cttctttatt caaatttttt ccaaaacgtt cgttgataat
    10681 aaacgatcca tagagcggaa agtattagaa ctaaaccgat cacaggaata ttcactaacc
    10741 aaaatggata aaacaaagcc aacattccgt ataagacgat aagaataata ctgatgatca
    10801 atgaacccat catagttgcg actaaaccca aacttcctga acctctgtta aataattggt
    10861 taatattggt ccagttcagc agcagcaacc gataatctct agcgaaataa cgtagtgaca
    10921 ataagtagca gccgaataaa gaaccaaata gcaagctagg gataaaaaac aaaggcagct
    10981 gaaaagacag tccgcttaat aatgcgatcc caccagttaa aaaactctgc aatatccatc
    11041 cgaatctgaa cttttctttt aaatattggt tcatagagat tggcaaagaa cggataaaca
    11101 gaaaattttc ttgatccaaa gaaataagat tgctgataaa agaagtctga ttaactgtta
    11161 agaaagccaa tgcgatccct accaggaaaa caacaccgat taacttatta tctaaatcag
    11221 ttaattttat ttcgcctcct agggcaaaag caagaataaa aatgattgga gttaacaagg
    11281 aagaagaaag aacttgcata atcaaattag gttcttttac taattgagca ttgtaactaa
    11341 ataaaagctg atgcacattt tggttctttt tatgggttct ttttattttg aactcactgt
    11401 ttgaatcagt cagttgctct gtaagctttg gtactatcag ctgttttaca gcaaggaaaa
    11461 atcccagagc cgtgatagct agtattcccc aacccaataa accttgttga gaaagtggct
    11521 gactggcagc atcatagaat ggcaataata tcgaaatcgg atgccgatct gttaactcat
    11581 tttcaatagt ccccgtttga tgattcatcc acatgattcc agcaaatgcg atcagcatcg
    11641 agctgccaag caagatacta gaaactgttt ttctatgttt tttaaacact gctgttcgag
    11701 ccaaaccaaa aacaatcaaa ctagccagac aaaaaagaat aatcaaaaat agagaaaaca
    11761 aaaggattga gataatgata gtaagcaaaa tccctagatc agctcgccat ccggttaaaa
    11821 taaacatgac tagaagcgga aaaacaaaag gaatgatggt caaagcaact accaaaatct
    11881 ttgctgaaaa aacctgttct tgtccaaaag gcagaggcaa aaatacttgc aggtcatttc
    11941 cttcaaaaaa gatattataa atgactgata tactttgagt taaacctaac ataccaaaaa
    12001 gtgccacgta ataagtaaag aaacctggca atttaacaaa gttcatagca aacatagcaa
    12061 atccgtaaat cagtaaaaaa atcagccctg aaaaaagatt ttgagacaaa agatagatag
    12121 ttaatcgctt tccctttttt cctttctctc ttgctttatt cgtgagctgt ggattaacat
    12181 ataaaagatt aactcgaatt aattctaata attgtcgctt atacatacgg tccatctcct
    12241 gtcgcttgcc ttccagccat tttcaaataa atcttttcta gcgtttcatc aggtgagcca
    12301 ttcaataatt cactaactgc cccctgataa atcaattcac ctttcttcaa aatagccagt
    12361 ttatcgcata actgttgtgc ggtatccaat acatgtgtag aaaaaatgac tgtttttcct
    12421 tttgcggcat gagccttcat catttctttc aaatcaaatg ctgcttgagg atcaagtcct
    12481 tgcaaaggtt catccaatac ccaaatatct ggatctggca gcaacgtgcc gatcaaaatc
    12541 gttttttgac gcattccatg tgaaaaactt gcgattgttt cattttgatg actatacata
    12601 tcaaaaagcg ctgtcaattc tgctaaacgc ttttcttttt tttgtgtgtc taattcatag
    12661 gcagctgaaa tcaagtccca atattctcca gccgtcaatt gtaaaaaaat atctggggaa
    12721 tcaggcacat aaccgatttt ttgtttgata ctcaatcgat tttccgtcaa tttcattcca
    12781 tctactaaaa tcgttcccga tgatggttca ataatactaa ctaaactttt aatcgtagtt
    12841 gattttccgg caccattatg tcctaaaaat ccaaaaattt ccccttgtct tatcgagaga
    12901 gacaagtctt ttaatgcttg ttttttcccg taacttttac ttacattgat gaattcaatc
    12961 atttctgtac tccttttgtt tgttattact ttttattgta cccttcgtta ttggtggagt
    13021 aaatgtcaaa aagagtgcat aacaaaaaag gatagaaacg actaggctgc tacttgggtt
    13081 gttctcttct acaggaaaac actcttctaa caggtaagat atgagtcttt gttttcttta
    13141 aaatgattca ttttctatct tttcttgcat aaaattcttt agtttatcaa tacttctctt
    13201 tcccatctct agttgttctc catttgcaaa tgttactatt tcgtttactc tgctaataga
    13261 tgtaatgttt tcagtattaa tgatataagc ttttaaatct ttacataaat actcatattc
    13321 gagtgatttt ttatattttt taattgtgcc gtttacgaga atctcttcat ctttcaatac
    13381 taggcctatt ttatttttat aacctggcat gctcattaga taaaggattt catcgtaatg
    13441 gatatagtga tcgctattac cataacggac agttgtccac ctttttttcg tagcatgttg
    13501 aacaggatag ttttcttttt ctttcaataa gtaactttgg atgctttttt tatttttttc
    13561 ataagtgaat tcttttatca aataaccact aggcgtaatc tttcgattaa taacagttat
    13621 tgctttattc ggatgagacg ttagataaac aattttacac ttttcattct tttctctaag
    13681 catttcgcct agttcgatac cgctataata ggcgttcaaa tcgatgtcta ggaaaaaaat
    13741 atctgaggaa tagatttcat tatttttaat gaattcaaca aattgtataa cgttcaatcc
    13801 ctgaacgatc tcaaatttcc aaggacttaa taaaattctg gattcttcca aaatttctac
    13861 tagcattttt tcaaaaacca aactatcatc caaaataaat atacgcgtta ccataaaaag
    13921 tacctccact cgatatttta ctatcaaaat catttcatac aacttttcaa catacatgtt
    13981 aggaataaat tgtaattctc ttcacaatct tttccaacac ctttgaaacc gatatcaaat
    14041 actttcagga tgctgcttct aaaataaata tcaaacaaat gaatacatca tgcaaaaaaa
    14101 gccagacaac cgacgtcctt gcgtgtcagc tgtctgagct atgtttattt attttgttct
    14161 tttaaaaaga acgatacaca tactgatttt tcggagcaga gactttctca gcgtttgtaa
    14221 caactacaga tgggatttca cgcttgaatg gttgataata gtctgattgg atggttcctt
    14281 ctattttata ccattcatca tttttaaccg ttactccttc cggcatatgc gtcaacaaac
    14341 caaagactcc tgaatctgcc acacaatgga taatgccgaa tcggaacaag aagatatctg
    14401 tattgctatc gttcggtgtt tggtaaacaa atccttcata gctgattttt ttgccaatga
    14461 attcgctagg gtaattataa attagctcca taacttctaa atagttttca tctgtgatct
    14521 tgatagtttg cttatcccca tacttttctt tcaactcatt catttggtct aagtagtctg
    14581 atttgttgaa ataaatactg gtatccggct ttaagtattg tgtctgcatc tccggatccc
    14641 ctactgattc tttacttact gggaaattaa atcctttggc ttctacgatc gtagtgtcga
    14701 gactaactgt tggaaacaac gtccccacga ttactggcaa agctagcagt acataagcga
    14761 ttccacgttg ataaggtttt gacaacccat ggtcatgatc gtgctcgtgt gccttttctt
    14821 ctgcttttcc gtttttgacc cacaaataca gctgaactaa agccaaaaga gcagataaaa
    14881 tcattgaaag aatagctaag tagcgataat ggacattgat atattgatcg agtttgccag
    14941 aaacttgcaa gtacatcatc aattcgaaat acccagataa aatcaaaaac cgcagcatat
    15001 tttccctcct aaataaataa tgtgaatcca ataacgctaa gggcaatcag tccgatcagc
    15061 gataaaataa agcgtcctgt aaaatatcgt ttcatcatca gtagattttt gatatcgacc
    15121 attgggccaa agaccaaaaa ggcagctaca ggtgctgtgc cgaacagact taacaaagaa
    15181 gatcctataa acgcatctgc ttctgagcaa agagacatcg taaaggccaa taacagcatg
    15241 atgataatgg ctaaaatctt tgttgagccg agcgttagca taattctagt tggtaaataa
    15301 gtctgcatcg cagctgcgat taatccgcca atgatcaaat aacgacctgt atcaaaaaat
    15361 tcgtcgattg aatgtgtcaa cacatgccca gtttgatgga tcagctgatt ttttggttca
    15421 ttcttctcac tttgttcttc ttcatggaca tgatgatggt gattttctgc tactttagac
    15481 gtgatttttt ctttcaatac agcgtctttt tgaaaatagg caagccagac accaatcaca
    15541 agggcaacaa aaaagctgcc tatcacccgc cagcttgcca tctgccaaga attgccaaat
    15601 gcgataaaag tggaaaaaat cacgattgga ttgatgatcg gagcagtcag cataaaagca
    15661 aacgctgtgt gaactggtac gcctttttta acaaattgat gaacgatcgg tacgatgccg
    15721 cattcacatg aaggaaagaa aaatcctaag atcgaaccag taaaaataga gaagatacga
    15781 ttttcaggaa gccatctctt cacacgttct ggggtcaaaa atacttgtaa ggcgcctgag
    15841 ataagacagc ctaataatac gaaaggtaat gcttcaataa cgatcgataa gaaaatcgta
    15901 ctcatctgca aaaccgaatt tggaataaaa gaaaacatga gtttcctcct ttttatttct
    15961 agttcattcg ctttgcaaat atacgctttt attcaaaaaa aacaagttgc tctgtaaatt
    16021 tttacacaac tcttattttt taccaaagca cttcaattga ctttttctgt tattcccatt
    16081 aaattaaggt ctcatacata tcacttctat cataaaaggg agaataaaaa tgaaacaaac
    16141 cactaacttg actgaagttc aagacatact gcaacagtca tccgttgccg tcatttatct
    16201 ttcgatgcct aattgcagtg tttgtcacgc tgttcgtcca cgactagagg aacttctcac
    16261 acattatgat attccggcac ttcatttgga tgcatttgaa acaccagaag tagctagtaa
    16321 atttgaagtg ctgactgcgc cagttgtcct gatttttcat caaggaaaag aagtctttcg
    16381 gcaagcacgg ttcatcgact ttaaaaaaat ccgttatcta ctagatgaac tgacagcaga
    16441 agatgattcc ctttcttatg aagaaatctt ccgaacaaaa taatcgttgt ccaaacagaa
    16501 aaaagcgatt gaacaagtga aagttttgcc ttcatttgtt caatcgcttt ttatttttat
    16561 tctgctgtat tttcggaatg cgcatttgcg tcagaatgca agaaatagat caaggtttgc
    16621 aattcattcg tcaaatcaac attttggatc aatacatctg acggagcatt caatcgagca
    16681 gcggtaaagt tcaagattcc tttgattcct gcttccgtta actgattgac tacttcttgt
    16741 gctctttcag cgggtatcgt taaaattgct acttcgattt gttggatctt gatttgttct
    16801 gacatatctt ccattggata aacggggata ccgtctacga tgcgcccaac gatttcctca
    16861 ttcacatcaa aagcacaact gatacgtatg ctattacttt gatggaattt gtattttagt
    16921 aaagcacttc ccaagttccc tacaccgatc agcgcaacat ttgtcaattg atcgtcgttt
    16981 aatgtctttg caaaaaagtg cataagatct tctacatcat aaccataccc acgttttcct
    17041 aattcgccaa aataagaaaa atctcgacgg atcgttgcac tatctacttg aacggcttca
    17101 cttaattcag ttgaagacac ttttttcttt ccagcattat ttaaaatcct taaatagcga
    17161 taatacaaag gcagtctttt agctgtcgcc tttggaataa tatgatcttt cacgaaatga
    17221 cctccacgta ttgtctaatt acatctttga tttctccatc atagcagtat ttataggaaa
    17281 aaatcaattc cattgctcag atattgtgaa attttattct ttattcattt atttatcatt
    17341 tctgcaaatc tcgcagtata atggaattta tggtagacta ctaattgaaa aatagttttt
    17401 tatttgagga gaattcacaa agtagaaaag aggattccat gatattatta caagccaatc
    17461 agattgctcg ccattttggt gcagaagtct tatttgaaaa catacattta gaaatcagta
    17521 caggcgctcg tgtcgcttta gttggcagaa atggcgctgg aaaatcgact ttattaaaga
    17581 tcattgctgg aattgaagca cctgatgaag gaaagatcgc taaaaataaa acagcgactc
    17641 tcggatacct cgctcaggac acaggacttg attccaatga aacagtttgg aacgagatgc
    17701 tgaaagcttt tgaacaagtt agacaaatgg aagagcggat gcgcgagcta gagattttca
    17761 ttagcgaagg tacgccagac acacctgctt atagttctta tttaaaagaa tacgaccggc
    17821 tgcaacacga tttttcagat cgaaacggct atggttatga aaatgaaatt cgttccgtat
    17881 tgcatgggtt taaattcgat tcttcatttt atgatcagaa aatcgatact ttatcaggcg
    17941 gacagcgcac acgtttagct ttagctcgca tgcttttgca acaccctgac attttgattc
    18001 tagacgaacc aacgaaccat ttagatatcg agacactttc ctggcttgag aattatctgc
    18061 aaagctatac tggtgcatta ttgattgttt cccatgaccg ttatttttta gataaagtag
    18121 tcaatgaagt ctatgaatta agcagaagaa aaatgcgcca ttacaaaggt aactattcga
    18181 aatatctaga tttgaaagca gaacagttag caagtgaatg gaaggcttat gaaaagcagc
    18241 aaacagaaat ccataaatta gaagacttcg ttgctcggaa ccttgttcgc gcctctacca
    18301 caaaacgagc gcaaagccgc agaaagacat tagaaaaaat ggaccggctg gatcgtcctc
    18361 aaggggatga aaaacaagca aactttctat tcagtataga aaagccttct ggaaatgtag
    18421 tcttgcaaat cgaagatgcc gctatcggat atgataatgt caccttatct gaaccaatca
    18481 atctagatat ccgtcgtcaa gaagcgattg ctctagtagg tccgaatggt atcgggaaat
    18541 cgacgttgct gaaatcaatc attcaccaga tcccttttat ccgtggaaaa gaaacgctag
    18601 ggacaaatgt caccatcggc tattacgatc aaggtcaagc tgatctgcat acaaacaaga
    18661 cgatcctagc agaattgtgg gatgagcatc ctaccactcc agaaaaagag atccgaagtg
    18721 ctttaggtgc tttcttattt tctggcgaag atgtagaaaa accaatccct ctgctaagtg
    18781 gcggtgaaaa agcacgggtt gctttagcca agttagcgat gaacaaagaa aattttttga
    18841 ttttggatga accaacaaac catttagata ttgacaacaa agaagtatta gaaaatgcat
    18901 tgatcgatta tgacggcaca ctgctttttg tttcccatga ccgatatttc atcaatcgga
    18961 tcgcgacaaa agtcgtcgaa ctctctgaaa atggcagcaa agtctatcta ggagactatg
    19021 attattattt agagaaaaag caggaagaag aaatcgccga attgttggca caagatgagc
    19081 cagaatctgc tcctgctccc acgaacaaat ctgattatta ccaaagcaaa gaacagcaaa
    19141 agcttatccg tagccttcaa cgaaaaatta ctcaggttga agaagaaatg gcgcggatcg
    19201 atgaatggat cgatcagttg gaacaagaaa tggtcgcgcc agaaaacctg gaagatcacg
    19261 taaaactcaa tgagctgaac caagatttag aagctgctag acaagatcaa gaaacaaaat
    19321 tgacggaatg ggaagaacta agcttggaat tagaagaata tgaaaattaa cgaaaaaacc
    19381 aaaacagaaa ccattcatgc tgttttggtt tttctttttt tattttatca aacaaaaatt
    19441 ccatatttac ctttttcacc aataagaaga ccaattacta gtcattctca ctaaagcttc
    19501 taaataatag taatcacccc aaatacaagg tgtatttacc ccattatttt tgggcttatt
    19561 ataaacagac tgtgttaaaa agccatcttc ttcttccgtt gtagcatatc tttctttcaa
    19621 ttgtcgcata attttccaaa cagcagactg gtataagttc ttttctgttt tgtctaatag
    19681 acctgattct attaattcta ataatccaca aaccatgatc accatactag atgtatcttt
    19741 ttcttctctg ctagaatcat caaagatcaa atcccaataa caaatggtat cttctggcaa
    19801 ccgattcata aaataattgg ctaatatttt gcttaaatca aaatattttt gttctttcaa
    19861 ataacgataa cttaatgcaa aaccgtaaat cccccatgct tggcctctag cccaacagga
    19921 attatcacta taaccttgat gcgtacttcc atatcttggt tttccatctt tgacatccat
    19981 ataaaatgta tgataagttg aaccatcttc tcttactaaa taatctgctg cattttgaat
    20041 atgagcctga gcagcttttt tatatttttg atttccagtt tcttcactcg cccaatacaa
    20101 taatggaaga ttcatgcaac aatccatgat cattctacct tgttgtgaag gatcactagg
    20161 atctccccaa gcttgtataa ctcctgcctt ctcccaaaaa cgctgaagta atgtttctgc
    20221 agcatcaatt gaagcttcct tcatttttcg atttcccgtt attttataat ctgctacggt
    20281 tgataaagaa tacaaaaaac caagatcgtg atgattagtg ataatttgac agtcaatcct
    20341 gtggataaaa tctatatctg ctttttcagc tgtttgttga aataaaggat cacccgtctt
    20401 ctcaaataac aaccaattaa ttcctgtcca aaatccttct gtccaaccaa tattgtctct
    20461 taatggataa cgtcctttta taggacactc gcttgggaat tgatactgac tgttactgat
    20521 acgtttgatt aacctctccg tgtgtctgcg acaacgctcg atctcattat tccattcttt
    20581 ttccattaat tctttataac attcatttat ccatttttct ggatgtatta tctcttcaaa
    20641 catcacttta tcaagcattt ccccaccagc ctttagcata attttagtct ttttttattg
    20701 tttttatttt ggatagttag cccggttgtc ccatcaaaat caattttggg acgattgttc
    20761 catacttcta aactataatc ccgatcttga tgagcaacca ctcctgttcc gagccaatgg
    20821 gttccttttc caaatgtacc ctctaatcca ggaataaaag tgttttccca tgtcatcaag
    20881 tttgtattgg gcaaacccat aatagctgtg gattttcgtt taatagtatc gtcacttaaa
    20941 cagataatac ctgaaaagcc agcttcactc accctatagt tttccttgtc ttcttctatg
    21001 attattcctt ctttctcagg agaacattca atagcaaacc ctgtctcata tagaacatat
    21061 tctgtatcta attctacttt gtgtacgcga atctgccagc cttcaaaagg aataagccaa
    21121 gttctgattt ttgcctgtaa agtaggttcc catgtagagg caagtacctc tttcttacat
    21181 acaatgtctc gtacattatt tcgaacgatg aactgtccat aggttttgat gctctcattc
    21241 ttaaaactgg tcgtgatata tgtgtccgct cgctgaatcc ctagcattga atccattgcc
    21301 atttctgctt tcaattgatt cgctcgcggt atcgaaaaac caaattgact agaataagca
    21361 aattttaaat atttttcatt ggtcaatgta tggaaatttg gccctaattg tcccgcattc
    21421 aaaaaaaatg tatgtccatt atcatgttgt atgagcaaat tggcttctga aagaagttta
    21481 gttccttcac gaataggcat aggcatttct ttagccgtcc aatactcatg gtcttcaggt
    21541 agcgccaaca ataaaaaaat cttattcagc cataatggag ataacgtact attatagggt
    21601 tcagtcaatg tcaattgagg gtaggtataa ccaatattca ggataccttt ttcatcaaat
    21661 atttcttttt gcatccacca tcttatattc cgattgacta accccttgat ttcacctaat
    21721 ttaaaaggca aaagatcggc ccacaacata gctgaccaaa agcttgaaac tgcaaaacga
    21781 taaatcatgc ttcgaccaaa aggcacattt gcaccatctt ccgcaaaaaa atgaatatag
    21841 tctctagaaa aatcaatagc tcggttctta tatatctggc taatgggctt ttctggttct
    21901 agtacgctat aaatcaaacc ataataatga aatgcaaaag gattataata atcttggcga
    21961 cctcttgaac tgtctcgata ccagccattt tgaagataac aattttctat attatcaaat
    22021 gcttctttca gtgctgattc tttaactgga taaccaaatt tttggataat tgttccgaca
    22081 attatcttaa aaaattgcca attaccatct gcacattcat attcaaaaat ttgcgagaac
    22141 caatgaataa tctggctttt ttctcctgta tctaattttt tccataataa ttgttcatga
    22201 tgatataacg ctaataacat tgcgggcatt tctaccatac gctggtctct ttcatgtatt
    22261 tctccccaat aatcgggatg ggaaggatct gttccagctt ttattcctga tagaattaat
    22321 ttcaaccatc gttcatctaa ttggtcaatt gcgggccccg ctccccataa aacccgagag
    22381 aatccttcca ttcctgcaat tttatttcca tatcctaccc cttgatgtat ataatcaatt
    22441 tgtgcgccac ttgctcgata aaacggctgg agtggttgat ttatcttttt aaataagtca
    22501 aagtaatcct gtctggtcct taatagtggt actggatgtt tcattgtatt ttctcctctt
    22561 tttactacca aatagctaat tccgactgtt ctaatcgaag taagccttca ataaaaaaat
    22621 aatcagaata gatcagtggc acattataag tcagttctct atggtatgct tctgctccgc
    22681 ctccaatgat cccatcttca tccatattcc aatttgcata gtgcttttct gtcttttgga
    22741 gtatattgat agcagcttca tgatacattt ttgatcgtac agaggaaaca tatcctgcta
    22801 attccagaag accgcatgca gcgcatactc cagcactggt atcaatattc gaatctttta
    22861 cagcaaaatc aatgagagga atataaccag ttttttcgat tttctgaata aaatttactg
    22921 ctatcttttc tgctatagct agatagtacg gattttttgt gtaacgataa cttaacgcga
    22981 atccatataa agcccatgca tttcctctgc tccaacaaga attctcattg tatccttgcc
    23041 cgccaagtaa ccgaatatat ttccctgatt ctgcatcaaa ttctgcgata tgcccggttg
    23101 atccatcttt cctcactaga tacttagctg ttgtatctgc atgctgccga gcaattttag
    23161 agaacctcgg atcaccagtt tccctcgaag cccaatacag taatggtaaa ttcatcatac
    23221 tatcaataat catccaacca ggtttatggt cattccatgc tactaaatag ttccctgaaa
    23281 tattatagcg tccagcaagt accgtagctg catgtaaccc tagttgtcga ctatgtttgt
    23341 tttgagactg ttgataatgt gcaatagccg atggtaagaa taaaaaacct acatcatgat
    23401 gaacgtctga aaaatggtac agggcagatt ctaataattg ttcttgttgt attgctgcct
    23461 ctttgaactt ttctcgtttt gtcttgtgaa agagctgcca taaaatacca ctccaaaaac
    23521 cattggtcca ccagtcgatt cctctgtcct ttattacgtc tgtatatttt ccgttgtaag
    23581 aaatatatgg aattttattc cccattctgt ctaattccgc atctatcttt tcagaagcta
    23641 cattcaaaat ttttcttgac cattccttcg tctgttcatt catggatatc ccgccattct
    23701 tttaattttt atgaatgtgg agttgctcct agaacgaact agatggttaa cattcgttat
    23761 tttttttact tatttgcagc tccaatgtca ttctccttaa atttagactg cgcccacaca
    23821 taaatcaaga ccattgggat cactgaagtt gctaaaatta gcataattgc gccataatta
    23881 attggctcac ctgtactaga aaccgtcata tttttgataa ttaagggaac cgtatattta
    23941 tctttatctg aaagcatgac taatggaaat agatattgat tccaacttcc cataaagata
    24001 agtaaaccac tagtaataat cgtaggtgat aaaatcggta cagcgatttt cgtaaaaatc
    24061 acccactcat tggcaccatc gattctggca gcctccatca gtgctgtagg aaattgactg
    24121 ctatattgtc gcatcataaa cactgaagtt gtactggcaa tcgaagggat gatcacacct
    24181 gcataagtat tcgataatcc taaacgactc ataatgtaaa ataaaggaat caatgtggta
    24241 aaaaaaggca ccatcctaga tagcataact gtattgaaca acagtttatt ccccataaaa
    24301 cgatactttg ctaatgcata acccgccatt gtagaactgg ctgtagctga aactgtactg
    24361 attacagcaa taacaatcga attccaaata atcaagggat aattatatct cgccatgatg
    24421 gcttgccagt tttttaatag catatcccca aacttgaaat tgattccgcc acttaaaatt
    24481 tcttgattac tttgcgttga agctacaatc atatataaat atggcatcaa aaatattaag
    24541 gcaccaaaca caataatgac gtacgtaaaa attctgcttt gtatcttttg tttagtcatt
    24601 tttatcacct ccgaatttaa attgaatagc cgataatata acagatacaa acaccatcgt
    24661 ccaggcaata gcagaggctt ttccaaaatc aaccatttca aaacttgttt tatataaatt
    24721 gactcctacg accaaagctg aattttctgg tccattggaa gtcccaaaga atatatttgg
    24781 gatattgaaa ttacccaagc cattgatagt ggctaaaact gtggccatta aaatgattgg
    24841 ctttagcatg gggatcgtta ataaaaagaa ttgccgaaat ttcgatgctc catctatttc
    24901 agcactttcg tatatctctt tagaaatccc ttgaagacca gctaaaaata aaatagtgtt
    24961 atatcctaca ctaagccaaa tcatcgtgat gatcattgat attcgtatcc aaaaaggatt
    25021 tgttaaccac tgtacaccgt taattcctag cgcattcaaa aatccattga ataacccagt
    25081 cggattgaaa aaagtcatga atacggaagc tacagccacc gttgatgtaa tggttggaat
    25141 ataatagaca gttctaaaaa atccttttcc ttttaactgt gctgaattca ataaaacagc
    25201 aaagtaaata gctaatagca tcgttattgg aattgatcct gccatgataa tcaaaacatt
    25261 cgtgtaagat ttaataaaca atggatctcg gaagatagct agataattgt caaatccaac
    25321 aaattcaaat gagtagccaa ttttcataaa actaaaagca aacgagataa tgatgggaat
    25381 caaaatcgtc acaataaaga aaataacaaa aggtgtgacc aatccataag cacttttttt
    25441 atgagacgta tctgttgctt ttttcttgct gttctttatt gtcttgcttg gtaacgaaga
    25501 ttgcatcatc gttaagtcct cctaatattt tttaatcacc agttaatttt acgccgtact
    25561 tatgagcaaa attttctgct tcttttagat atgaattttc aaaatcacct gtttgccaat
    25621 agttatatgc agcggtagtc aaataagacg tagcatctga ataagtggct ggtaaaacag
    25681 tatttgatgt attttcattg attttattca atagttcata gtatttttgt cctccttagt
    25741 agtcaaactt tttctcagct gcttctgttt gataggccaa tgagttagat gtagccacgc
    25801 ccagctccag tgcgttggct aagcattctt catctgtcag cataaatgta agaaattgtt
    25861 gtgccacttg tggatttttg gcatctttag ggacataaaa acttgatcct cctgatactg
    25921 caccttttcc tggattttct tttgaaaaag gaatcagtgg agccatgcgc catttgtcag
    25981 atgcatcagg aaaattcaaa gacatattcg tagcatacca gccgccagca acaaacattg
    26041 cagattcttg aaatgctttc tcagcatctt gtgcaccgta gaaatgtaca atatctgagt
    26101 cgatcatttc tttgattaac tttgcagcat gtttcgcttc tttagtattc agacggatat
    26161 ttccttcttc atcaagcaaa gattgattct gctgggctag catgttgata aacaattctt
    26221 ggtccccaga ttctggcaag gcaattccaa aactaccagt tttttctttt aatgcttttg
    26281 ccacctggat atattcttcc caagaactga tttcttcaaa atctacacct atttcagcaa
    26341 atttttttgg ttgataataa caaagtgctg gggtaaaatc actagcccaa ggaataatcg
    26401 tttcattact cgtttgttga cgcaaaatat tcaatttttg atcataaaac tgatcgccat
    26461 atttgtcata aaatccatat tcatcagcac tataaaaagc atcttgaaat ttttccagca
    26521 cacctgctgc tgaagtgtct gccatggaga taatatccgg catatcttcg tgtgttacca
    26581 attttggggt gatcatttgt actgttgttg ttcccgaagc tacattcaca atttttaagt
    26641 caatatttgc tccagttttg cggttataga tttttgcagc attttcaaca tagtttgtca
    26701 gtttgccgtt tgcgacatat acttctaatg tagtggcttt ttttatcatt ggatctgcga
    26761 tgtttgctgt tacttcttcg ttagtttttc cacatgcact taagctgaat aatagggtaa
    26821 tgtttagcaa agctatcacc aatttgtatc tttttttcat gtctatccct tctttctttt
    26881 caagttctta tataaaacgc tttcacttat gtatattaac ggaattagta acacaaatat
    26941 ttatataaaa aaatatttta cttgtcgttt tggcttttca tttttgttat aatagtcatt
    27001 aattataaat agaaagcttt ttatataaaa agagaaagga aagtggttct tttgttaaca
    27061 aacaaccaat cctccaatct tgtcaaaaaa acactaacct attccataga tacccatctt
    27121 catttatctg ctgctcccta ttttcgacta ctaataaatg aaagcgctaa tttatatgct
    27181 gaatttgcac ataaatctat agaactagat aatccttccc ttttattcat tgataaacag
    27241 caagaagtcc aaatcttttc tactcaaaag gaaacacaaa cttttatcat tatccagttt
    27301 tattttgatg agcaattatt agcacatatt acgggacaat ctttttctat agatcaagaa
    27361 ataacatcta tcattacttt tctgaaaaat gaacagttca aaattgatta tctaaaacat
    27421 agtaagccag ctatttccta tgatcaggaa atcaattctt ttttcatact atgccaacag
    27481 atgagcattc atttgctttc cttattagtt acttattgtc tgcaaaaaat tcaattgtct
    27541 tccttaccaa ggcattatca acaggaactt gctttattga caataccttc aaatgagaaa
    27601 gtcattccta aaaaaaagca tattctttat tcagatgaat tagttgatcc aatcatacga
    27661 tatttagaag aacatctttc agaaaatttt ttgcttgaac aattagccca aatttttttt
    27721 atcagtccag ccagcttaaa aaaacatttt agaaaagtga atggaaaaag tgttattagc
    27781 tattttaaag aactaaaaat ggaaaaagca aaacgtctcg tgctagaaaa tgaattaacc
    27841 tatacagaaa ttgcagatat attaggattt cattcgatcc atcatttttc ttcagctttt
    27901 aaaaaatata cagggttatc cccttcaaag tattatctat ccttaaaata gaaaaaaatt
    27961 tttaggtttt atccacaaat tatttctcta tattttctgt ctattcaaaa taaacaataa
    28021 ttttccggca caattatttg ctgtttttta ccataacata gtacgatcaa cgtagaaaag
    28081 gagatgtttt tatgaaagtt gttgtcattg gcggtgtagc tggcggtcct tcatttgcca
    28141 ctcgtttccg tcgattgaat gaagcgcacg aaatcattat ctatgaacgc ggagaaaata
    28201 tttcttatgc aagttgtgct ttgccttatt atttaggagg tgtgatcact gaccgtgact
    28261 cgctgatcga acgtacacca gaaatattga aaacaaaaaa caacatcgat gtctttacta
    28321 aacacgaagt aacagcaatc gatccttcta ctaagcgatt aacagttaaa gacctatcca
    28381 caaatgaaga aataaaaaca gattacgata agttgatcat ctcttctggt gctagaccag
    28441 attatccgga tattcccgga gtttttgaag cagaaaacgg ctttgtcctc cgtagtgtga
    28501 cggatgcgga tcgaatcaaa tcgttccttg aagaaaaaaa tccacaacat gtcgtcattc
    28561 ttggtgcagg tgttatgggt ctggaattag ctgagaattt caagcatcgc ggcttagacg
    28621 tgactttaat cgatcaattg ccacaagtcg ctttccctta tgatccagaa attgctaatt
    28681 tagtttatga caaattgctg aaagaaggat tagccgttca tttagaaaca agagttactg
    28741 agatccgtga taaaggtcga gaaatagtat tatcagatgg ctccgtcctt tctgctgata
    28801 tgctaatttt tgctgttggt gtttctccga agaatgaagt ggtgaaagca accggcatac
    28861 aattatcaga tacaggtcag atcattgttg atgaccagtt acaaaccaat ctgccggaca
    28921 tctatgcgat tggcgatatt atcgaaacga ctagtgtagt gactggtcag ccgatccaaa
    28981 gtatgctttc cagtgcggcc aatcgtcaag gacatatgct ggcagatatt ttaaatggta
    29041 cgcctatgcg ctatcgcgga tatattggtg caggtgtcgc aaaaatcttt gatcatacag
    29101 caagttatgc gggaatgaca gaacatgaat taaaagcatc aggcataaca aattataaaa
    29161 ctgtttttat cactcccttt gaccatgcct atttctatcc agaagctaca agattaaatc
    29221 taaagctgat ttttgatgca gatagcggtc gtattttagg tggacaagca tttggagaaa
    29281 aaggtgtcga taaacggatg ggagaacttt ctgtagcaat caccggaaac ttgacagtct
    29341 ttgatttgcc tgatttggag ttgccttact ctccaccgta ttctactacc cgtgacccgt
    29401 tgaatatagc tggttatgtc gcaatcaatc aaatgacgaa tatcgtagaa acgatcaaag
    29461 caagtgatat acccgaaaac gatttgaaag aagcgttctt tttagatata cgtgaaccta
    29521 ataaagcacc ttctggcagt atctcggcaa caaaaaatat tccaatgaat gagttacgag
    29581 atcgaatcaa tgaaatccca aaagataaaa aagtttacat tactttcaga agaggattga
    29641 atacttatac ttctgcccga atcttggcag gtttgggtat caaagcgatt ttgattgaag
    29701 aataaaaaaa cgaccgcggt gggtagccgc ggtctaggag ttatttttac ttgaggagta
    29761 aaaatgaaaa agtatatagg gttgttggtt ggtatgtata tagaatagaa gataaatgtg
    29821 aagaaattgt gcattatttc ttcacattta ttaggttata gtttaagaaa gtttaaacat
    29881 tgcgcaattt aattatttta gctgtttctt ttcataaaaa aacatgttgt gactctgaga
    29941 caaatcatag tccatagtga tgtctctaga gtaactacgc ctaatattac gaggcatcat
    30001 attaatctat cagcactagt acctaaacta gcatcccttt cgaatatttt ttcttatata
    30061 ataaagtttg ctttcactcc tttaatctca ccaaatcgac tggcttgcgt tgtgtgatac
    30121 taggaactga ttggtttaga acgacttttc tattgtaatc atatttttcg ccatcctcgt
    30181 tgtcttctaa tccttcaatg aatgatccct aatgtgtgta ttaaataatt taattttttt
    30241 gttcctattt atttaaca
//
LOCUS       DACCKU010000027        29605 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.029, whole genome shotgun sequence.
ACCESSION   DACCKU010000027 DACCKU010000000
VERSION     DACCKU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.029"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(81..1376)
                     /gene="purB"
                     /locus_tag="IVN13_001141"
     CDS             complement(81..1376)
                     /gene="purB"
                     /locus_tag="IVN13_001141"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP7011641.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1389..2522)
                     /gene="purK"
                     /locus_tag="IVN13_001142"
     CDS             complement(1389..2522)
                     /gene="purK"
                     /locus_tag="IVN13_001142"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP7011642.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2506..2994)
                     /gene="purE"
                     /locus_tag="IVN13_001143"
     CDS             complement(2506..2994)
                     /gene="purE"
                     /locus_tag="IVN13_001143"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP7011643.1"
                     /translation="MAAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3410..4717)
                     /locus_tag="IVN13_001144"
     CDS             complement(3410..4717)
                     /locus_tag="IVN13_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP7011644.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4720..5301)
                     /locus_tag="IVN13_001145"
     CDS             complement(4720..5301)
                     /locus_tag="IVN13_001145"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP7011645.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5417..5514)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5873..7165)
                     /locus_tag="IVN13_001146"
     CDS             complement(5873..7165)
                     /locus_tag="IVN13_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7011646.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7162..7785)
                     /locus_tag="IVN13_001147"
     CDS             complement(7162..7785)
                     /locus_tag="IVN13_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011647.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7788..8624)
                     /locus_tag="IVN13_001148"
     CDS             complement(7788..8624)
                     /locus_tag="IVN13_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7011648.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8641..9528)
                     /locus_tag="IVN13_001149"
     CDS             complement(8641..9528)
                     /locus_tag="IVN13_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011649.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9544..10827)
                     /locus_tag="IVN13_001150"
     CDS             complement(9544..10827)
                     /locus_tag="IVN13_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7011650.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11510..12193)
                     /locus_tag="IVN13_001151"
     CDS             complement(11510..12193)
                     /locus_tag="IVN13_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7011651.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12211..12828)
                     /locus_tag="IVN13_001152"
     CDS             complement(12211..12828)
                     /locus_tag="IVN13_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7011652.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12838..13662)
                     /locus_tag="IVN13_001153"
     CDS             complement(12838..13662)
                     /locus_tag="IVN13_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP7011653.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13655..14215)
                     /locus_tag="IVN13_001154"
     CDS             complement(13655..14215)
                     /locus_tag="IVN13_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011654.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14388..15344
                     /locus_tag="IVN13_001155"
     CDS             14388..15344
                     /locus_tag="IVN13_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7011655.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15649..16605)
                     /locus_tag="IVN13_001156"
     CDS             complement(15649..16605)
                     /locus_tag="IVN13_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP7011656.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16709..17311
                     /locus_tag="IVN13_001157"
     CDS             16709..17311
                     /locus_tag="IVN13_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7011657.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17399..18898)
                     /locus_tag="IVN13_001158"
     CDS             complement(17399..18898)
                     /locus_tag="IVN13_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP7011658.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18914..19438)
                     /locus_tag="IVN13_001159"
     CDS             complement(18914..19438)
                     /locus_tag="IVN13_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011659.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19736..20485
                     /locus_tag="IVN13_001160"
     CDS             19736..20485
                     /locus_tag="IVN13_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP7011660.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20476..21261
                     /locus_tag="IVN13_001161"
     CDS             20476..21261
                     /locus_tag="IVN13_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7011661.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21230..21946
                     /locus_tag="IVN13_001162"
     CDS             21230..21946
                     /locus_tag="IVN13_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7011662.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22048..23067)
                     /locus_tag="IVN13_001163"
     CDS             complement(22048..23067)
                     /locus_tag="IVN13_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP7011663.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23086..24144)
                     /locus_tag="IVN13_001164"
     CDS             complement(23086..24144)
                     /locus_tag="IVN13_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP7011664.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24365..25513)
                     /gene="nagA"
                     /locus_tag="IVN13_001165"
     CDS             complement(24365..25513)
                     /gene="nagA"
                     /locus_tag="IVN13_001165"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP7011665.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25589..26425)
                     /locus_tag="IVN13_001166"
     CDS             complement(25589..26425)
                     /locus_tag="IVN13_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7011666.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26609..27826)
                     /locus_tag="IVN13_001167"
     CDS             complement(26609..27826)
                     /locus_tag="IVN13_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7011667.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28209..28292
                     /locus_tag="IVN13_001168"
     tRNA            28209..28292
                     /locus_tag="IVN13_001168"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28243..28245,aa:Leu,seq:gag)
     gene            complement(28532..29530)
                     /locus_tag="IVN13_001169"
     CDS             complement(28532..29530)
                     /locus_tag="IVN13_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011668.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8850 a         6313 c         5071 g         9371 t
ORIGIN      
        1 gcagaaccgc taacggatct gcttcttttt tggttatttt tgctattcta ttttaattga
       61 attttgcaac tcactcttct ttattccaaa cctactcgtt tgaatatcac gtcaacgttt
      121 ttcaaatgat gatgataatc aaaggcatcg tctatttctt ctttagataa taccgatgtg
      181 atggttggat cttcttctaa cagcgggcgg aactgcactt ggttgtccca agcgtacgca
      241 gtttttggct gaacaagatc ataagcttct tctcttgtca ttcccttatc gatcaatttc
      301 agcataacgc gctggctata aatcaaacca aatgttgcag acatattccg cttcatgttt
      361 tcaggaaaga ccgttagatt tttaacgata ttgccaaaac gatttaacat gtaattcaat
      421 aaaatcgtgg tatcaggcaa gatgattcgt tctgctgaag aatgagaaat atctctttca
      481 tgccataatg tgacattttc ataggcagtg accatgtgtc cgcgcaccac tctggctaga
      541 ccagccatat tttctgaacc gatcggattc cttttgtgcg gcatagcaga agaacctttt
      601 tgacctttcg caaaaaattc ttctacttca cgtgtttcgg atttttgcaa tccgcgtatc
      661 tctgttgcaa aacgttcaat acttgtagca ataagcgcca tcgctgagaa atattcagca
      721 tgtagatccc gagggagaac ttgagtagag atttcttgtg gacggatccc caattgttta
      781 catacatact cttcaataaa tggcgggata tttgcgaacg ttcccactgc tcctgagatt
      841 tttcctgctt ctactccttt tgccgcatgt tcaaatcgtt cgatgttccg tttcatttct
      901 gaataccatg tggcaagttt caatccaaaa gtagttggtt cagcatggac accgtgtgtc
      961 cgacccatca tcactgtata tttgtgttct tttgcttttt cgccaatgat ttccgtaaaa
     1021 cgtttcaagt cttcccgtaa aatatcattt gcttgcttga tgagatagcc ataagcagta
     1081 tctacgacat ccgtacttgt tagaccataa tggacccatt ttctttcttc tcccaatgtt
     1141 tcagaaaccg ctcgtgtaaa cgcaacgaca tcatggcgtg ttaatttttc gatttccaag
     1201 atccgatcga tatcaaatga tgcatgctca cgaatttttt tcacatcttc ttgcgggatt
     1261 tcaccaagtt ctgcccaagc ttcatctgct aaaatctcga cttcgagcca cgcacggtat
     1321 ttgttctctt ccgtccagat attccccatt tcagggcgtg tataacgttc taacatgttt
     1381 tttctcctct aatcccaaat attcgtttgt ttgatttctt ccagcgtttg ctggatgtct
     1441 tctgtcaaga tagtgatatg tcccatcttg cgaccttttt ttgcttgttt ttttccatag
     1501 aaatggaatt gccagtctgg cttattctca atcaagcgat agctttccag cagttgttct
     1561 cccaaaacat tgaccattac tgcattgctt atcaaacgta ctttttctcc taatggccaa
     1621 ccacaaatac ctcgaatatg ggcatcgaac tgactcatag aacaagcttc aatagaataa
     1681 tgtccagaat tgtgaggacg aggagctaat tcattcacat atagcccgcc tgtttttgtt
     1741 aaaaacattt ctacccctaa aacacctgaa aggctcactg cttctgcaat cacacgggca
     1801 atacgctcag cctcctcgat cacatcctga tcaatcgcgg ctggagcaat cgtttcatga
     1861 agaatattgt tgtggtggat gttttccacg accggaaaag tcgtgaaatc tccttgacca
     1921 tttcctgcca ccatgatcga aatctctttt tcaaacggga tccatgcttc taaaacacag
     1981 gttccttctc ttaaaaggtc catggcagga gctagatccg cccgactttt caaaacatac
     2041 tgccccttgc catcatagcc gcctcgagtg gttttcaaaa cacaaggata gccgatcccg
     2101 tcaatcgcat cttggatatc cgtcggactg acgatcgttg cataaggagc aatcacgatg
     2161 ttattcgttt ccaaaaaaga tttttctaac aggcggtctt gcgtaatcgc aagcaagtct
     2221 gtcccttgag gaataaatga catgggtaaa atcgcattta acgcctctac actgacgttt
     2281 tcgaattcat aggtaatgac atctgttctt ctggcaagct ctttcagcgc aaaggtgtca
     2341 tcatagacac tttcaatgtg ccaatctgca acttgtgcgg ctggacaatc gctcacagga
     2401 tccagcacac ctactttaaa gcccatttct tttgcactaa tcgccatcat acgacctaat
     2461 tgaccgccac cgacgatccc gatcgttgaa ccaggcaaca aaggtttaac caagttgatc
     2521 actactttct ataacagttt tagccaacat tgaacgtctt tcagccaatt tagcctccaa
     2581 ttctaaatca tacatggaaa gcatctgtat cgcaagtagt ccagcattaa ttgctcccgc
     2641 ttttccgatt gctgtagtag ctacaggtac tcctccaggc atttggacaa tcgaaagaag
     2701 cgaatccaaa ccatttaatg ttcgtgattg gaccggtaca ccgataaccg gcaaagttgt
     2761 ttttgatgcg accatacccg gcagatgcgc ggctccccct gctcctgcaa taatgatttt
     2821 gattcctttt tttcgagcgt tttgtgcata ttcaaacatc aaatctgggg ttcgatgagc
     2881 agaaacgacc tttttttcat aagacacacc gaattcatcc aatatttgac atgcttcttt
     2941 cattgtttcc caatctgaag tactccccat aatcacagag atttttgctg ccattgtttt
     3001 tcctcccagt ttttctacat tcatatttta acaatcactt tttttgatgt caaagaaatt
     3061 tcgaatgtta acaaagaaaa acattttatt gttcgtgttt attaaagata aaaaaaagga
     3121 atgtgacaaa agttaggtca cattcctttt tatggataaa cggtgttctc ctgccaactc
     3181 ctcggtcaca aggaacaata ttcaacaaac atgagaagct tttttttgaa attgttcctt
     3241 gtgactcgga gatgaacggc aggctcacga cctcttcctt caacggtgtt caatcagctg
     3301 accctcggta ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc
     3361 tctaacaatc aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga
     3421 aggcttcttc caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga
     3481 tagccgtaat gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca
     3541 tttgactgaa taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa
     3601 caatcaatag atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga
     3661 ctgccaccat cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt
     3721 gtgctaacgc accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat
     3781 agatgggttt tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc
     3841 cagtataagg gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat
     3901 atcccttttt caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat
     3961 atacacctgt tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc
     4021 atacgtcgaa agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat
     4081 ctccaatcgc gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta
     4141 aaccaatcaa taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa
     4201 ttaggagaat cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg
     4261 ttgcattacc tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg
     4321 tgataaccgt tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag
     4381 aaactaacac aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt
     4441 cattggttcc gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag
     4501 gcaagccaat tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca
     4561 taaagatatc aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac
     4621 cgatcaaaag aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta
     4681 aaaccgcagc ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa
     4741 ctcaacttgt ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat
     4801 ttcttcaagt aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc
     4861 tacttgcgct ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc
     4921 caagaaatcg tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga
     4981 aatcgtactt gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc
     5041 catcgtcaaa ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc
     5101 aaataaagct ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc
     5161 tttaaatact tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg
     5221 agtgacgaag ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat
     5281 acgttcaact aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac
     5341 cgcttttgtg caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat
     5401 aagaaaagaa gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc
     5461 tagaaactgt catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg
     5521 taaattcatt ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa
     5581 aatgttgttt caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt
     5641 tacttatttt tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt
     5701 aaatagttat ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga
     5761 caaaaagcat gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg
     5821 caataaggca agattttata aactacggga agtaatttcc gtaaatctat tcttattctt
     5881 ttaagctgac aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg
     5941 cgattcctgt caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat
     6001 cttcttcttt ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc
     6061 cgcccgcttc ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga
     6121 tggactgaag aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact
     6181 tttcgatcac tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt
     6241 cccacggcat atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg
     6301 ttacgcctag tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat
     6361 atccactgtc tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt
     6421 ctatagcaaa tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt
     6481 ctactccgcg tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata
     6541 ccttttgggg acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat
     6601 gaacaagaag cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc
     6661 cgtgttgcgt atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac
     6721 tttctatcag ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc
     6781 gcaggcttct tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga
     6841 tagtcagagg tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat
     6901 catgatgcgt tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta
     6961 gttgctcaaa ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa
     7021 gtatccgatt tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac
     7081 aagtttgatg atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc
     7141 cgacttgtac atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac
     7201 tgtgacaaat ggcagccctg gatacagtaa tgcagtgagt aaaaaatcat gctgtataca
     7261 ccagaacagt ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa
     7321 cagccagata ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat
     7381 atcaattagc gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg
     7441 aatcagatac caagctccaa tctttcccac taataaatac caatcaactg caaaaataat
     7501 gacaaacagc tgaaacaacg taatgatgaa acacatccgt ttgatttttc ctttcctcca
     7561 acataccttt ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa
     7621 aaaataaatc agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc
     7681 tagaactaaa gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata
     7741 gtagttgagc ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga
     7801 ccgcttgtac tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc
     7861 ggtagcagcg acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg
     7921 tctgcaaata atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc
     7981 aatggtccca tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc
     8041 gaaggtttca gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca
     8101 ataatcgctg attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa
     8161 atgaaaaatg cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa
     8221 ttcagccgat tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc
     8281 gttactaaca tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg
     8341 taagcaatac aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac
     8401 gaattcctaa agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa
     8461 ttttcccatt gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc
     8521 gttttaaaag aacctgcgat catccataag accggataaa tcatgactag acctaatagc
     8581 aaaataaaac cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat
     8641 tcacacaacg acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat
     8701 caacagcatg atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg
     8761 gttatacaca taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat
     8821 aaatgcctgc gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat
     8881 gatcggcgaa agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc
     8941 gatctctgcc gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat
     9001 cattgccgaa ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg
     9061 atcgctaaac caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc
     9121 gtttttagaa aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact
     9181 aggcacataa tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata
     9241 agcaatcaat agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt
     9301 gactcggatg gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc
     9361 gatccatgag actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat
     9421 catcggtatc actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc
     9481 gaccacattg tcttgatcca agaaccgttt tagtttttct tttttcatgc cttcctcctg
     9541 ctgctatcct agaatgtttg acgcaaactc ataaagactc tctgctgctt cttctgctgt
     9601 tacttcttca taaatgactt tttcaactaa aagattgtat tggtcttgaa tttccggatt
     9661 caaagcacta tcaaagacat tcacttcgcc gttgtccata tcggaaatca tttctaaata
     9721 atcaaatgtc gcttgcaaag ttgcatcatc tgcacgagcg agctgttctc gaatattcga
     9781 catgattggt acaccacgtt ctccttttaa aatttcattg gcttcttcac tgttttgaaa
     9841 gaaatcaata aacttagcag cttcttcttt atgggctgag ctttttgcca tactcagcat
     9901 ttgcgaagaa cgtaaaggga taccagattc tccatctgct tttctgcgag gtggattaat
     9961 caactgaatc tccatatctt caggagcccc tgacaatatt tctgtcaact ggttacttga
    10021 tagaaacacc attgccgctt ctctatccct aaccgcctga aaagacagat ccgtcgtact
    10081 tgcagctcca ccacctggtt ctggatgggc ccctttgtct accagatttt tacgcagctg
    10141 gaaaaaatct gttagatagt ctggattttc aaatcccaag ctgttagata gatcttcccg
    10201 attataaaaa ttcaatcctt tctcaacttg tggtatttga ataatacagc cagctataaa
    10261 atcttcgaat cttgaaatag catagattcc taattcttca gtgatttgat agctgatttg
    10321 ttcaaattca tcccatgtcc aatttgaagc tggttcaggg atatttaatt gttcaaatat
    10381 atttttattg taagcaatcg caaacgaatt caccccgtta gacagtccaa ctaattgccc
    10441 gtcgtaagtc gtcgttgcta aataggattc atctgcatta gaaatatcaa ttatcccttg
    10501 ctccacatat tcatctaatg gttcaatctg attgatgtat tgaggaaatt gattgttcag
    10561 ttggaaaatg tcccagatat catctgatgc tgctagtgta cctaattttg tgtaatatcc
    10621 ttcataatca taaaattcat aatcgatcgt cacatgggga tttttttctt cataaagctc
    10681 gattactttt actgttgcat cgtgtcggac ttgcgaaccc caccaagcca tgttcagcgt
    10741 gatttttttg tcatcttctt gcccacaagc cgtcaagaag aataacataa cacttaaagc
    10801 taagatgatt ctcattcttt tcttcattct ctgcacctct acttccattg ttatttttca
    10861 atatactgta tttttataca gaacatattt attaaacgat ctttttcaag gggaataaaa
    10921 accaagactt gccttatcgt tctgcctagg atgaacaact caatactttc aagaaaaatt
    10981 agatagtgct gattcttctt tcatcgctaa aaacacttct tgcttcaatg atgggaggat
    11041 aattacgcaa agaaaaaagg atatcagaca actgcccaat atccttgaca tagctaaata
    11101 agcatgcttc ttcgtgtgcg ctcttcccta aaaaacagaa aaataactag taattctgta
    11161 tgaaattagg tacaatatac acgtcgatgc acaagcattg actatggcaa cttgattggt
    11221 cagttcccgc tgacttttca agccccgaca atttagctgc tactaaattg tcgggtgcct
    11281 tttttagttt ttattcgttt ttcattctat agaaagcgct tcaataaatc aagacatttt
    11341 tttgttagaa tgctaataat aacaaaaggt tttaactctt cttatgtaca ttacaggtaa
    11401 aaaaacaaaa aaatttctct tttttttgaa ttctgaacaa atagtactgg agtacaaaag
    11461 acgaggaaga gccactatct gctcttcctc gtcttttgat tctcacctgt tattcatgat
    11521 tctttttctc agtttcccga tatttttcca ttttacgact aaaaagctct ccgctacttg
    11581 gtggcgtata tgtgatggca tttgctcctg cttgaatggt ctcagcaata ctttcttccg
    11641 ttggaccacc tgtcgcaatg ataggaagtt caggatattt tttacgaaaa tggcgtactg
    11701 cttctgcagt ttctttacct gcactgatat tgataatcga aataccagct gctaactttt
    11761 catctatttc tgtatatttc gacgttaccg tactgataac tggaatatca actactcgac
    11821 aaacttcttt tactgtctct aaaggcgtag gaccgttcaa aacgacccct aacgaacctt
    11881 gcgcttctgc gaacatgctc atgtaggaag aacgagcacc ttgtgtcaaa ccaccaccta
    11941 caccggagaa aactggaatg tcagctgcct caataatgct tttagtaatc gctggatgag
    12001 gtgtaaaagg atacacagca attactgcat tcgcatttgt attacgtatg atcgcaatat
    12061 ccgtagtaaa gatgatcgaa cggatcaatt ttccaaatat cttgatcccg cttgcttcta
    12121 aaatgatttc cggtactctt acgatatctt ttctcaattc cgtcatgatt tctggcgtaa
    12181 attgttctgc catcagccac ttctcctttg ctatctccgg tcatactgat tcaattgata
    12241 cgtctcgatg acctggatga ttttttgcgc ataaccgggg tctgtcgcat aacctgcatc
    12301 ttgcaatgct tgtgcggctt cctgatagtt ggtggccgta atcacttttt catacagcgc
    12361 aggattccaa tctactcctt ggacgaacag ttgtgtatgg tcatccatgg actgttccca
    12421 tgaatcatac actttgaagt ctccttggat agttatccat tcttcgttaa caaattcctt
    12481 tgtttccagg cttactttct tctgatcccc ataagccttg atcccgaaaa gattattgta
    12541 tttgctagcc aaggtacttt ttccccaatt tgattctaat atagcttgtc ctaaaatgat
    12601 actaggaagc actccataac catcttgtag ttcttgggca tgcggaacaa gccgttcaat
    12661 aaattgctga tgggattgcg cttcattttc ttgctgatta tcagtaaaag gagaagacag
    12721 attcttcaag gaaaaaacaa aggctaagag tatcactgct acaccggcaa ccactgctgg
    12781 ccaacggata ttcttgcgct tttgcttttt ccactttttt ttcgccatga tcaattccta
    12841 cttttcattt aattcattca gatattcttc tagcgtcaat tggtgtttgg tgatatactc
    12901 agcgttttct ttaccgacat aacgtaagtg ccaaggttca tatgtgatct tagttatatc
    12961 ttcgtgtcct tttggataac gaacgataaa tccatactgc gcagcattat ctgcgatcca
    13021 tttagctcca ggctgatctc cgtaacttgc atccaataca gtagaaggat aactgttgta
    13081 ccagttttga tcgacgacat cgactgccag acctgtgtgg tgttcgctgt atcctggctc
    13141 cgtaatcgtt tctttagtaa tctttgtcgc ctcctcttct gatacacctt gacgactcat
    13201 cacatcttgg acattttgtt cgaaaacttg ttgttgggat gatacagacc ggtaagctga
    13261 gaccatcact aatggaaatc cagctgcttc agcagccttg gcaaatgctt catattctgg
    13321 agcaatgcgt ttgtcgatca agtatccgtt acttaatgat gccaattgag tagactcatc
    13381 aatttctttt tctaatttgt gatctggacc aaccaaaact agcttccaat cgtctgtttt
    13441 aacatctggc aattgtttgg acgtacgatc ttttgtttct ttgtccttta ccttgctgct
    13501 ggttgtttga ctgttctttt ctttactagc agttgttttg gtgcttgttc gttcgactgc
    13561 ttgattgccg gcttgaacat gcgaatcatc atcgctaaaa ataacatagc ccattgctgc
    13621 gatcataatc acaactgctc caaatcctaa aaatttattc acttgaaaaa tcctttcgtt
    13681 gttcttcacg ttccccattc gcctcatgaa cgtaagtttc ttccagattt tcattcaccc
    13741 aatcgagaag ttcgattttt tctccttcga tctgtttttg aacatctgct ggcagacgaa
    13801 aattcaaaat atcatcatag ccccattcat catattgaat acttactttc aagtcaaggt
    13861 atctaccagc ctgctgatta tttttcaaag ggatcagctc gacttttgct gctttgctgc
    13921 taagccattt ttgcgcgatg cgtgttttga tttgcttgta ctctgcaacg gcaacgtttc
    13981 gtttttcggg aaaaataatc gtttgacgca aaatcttttg cgttagcatc cattcataat
    14041 cagtgaaaag attttttact ttctgcatcg cttcagatgg cagatttttc tcgccatctt
    14101 tccatttttg ccattgttct ggactgatca gcaagtattc atctaaaaaa tttttttcag
    14161 tttcaaactg ttctaataat acactcataa taagatcaat atatacttga tgcacgttta
    14221 tcccctcctt ttaactattc tacttaagtt ttgatcaaaa agcacctctt aaatcgctgc
    14281 tttcattttt ttaatgtttc aagtcctctc ctctttattc ttcgtgattt ttgttataat
    14341 aatagacaga caagaaactc aatccaacta tgaaggagcg ttttcagatg aaaaaatatc
    14401 aatggggaat cgttggtctt ggtaccatcg cacatgagtt tgccgaaagc ttcaatcaag
    14461 agacgagcga actaacagca gttgcttcca gaacttcaga aaaagcggaa aacttcgctc
    14521 accgttacaa tattccaaaa gcttacggaa gctatcaaga aatgttagac gatgctgaaa
    14581 tcgacatcgt gtacattgct gtaccaaata gacagcatat cgaccatatc ttggcagcat
    14641 tggaagcagg caaacacgtt ctatgtgaaa aagccatcac gatgaataaa aaagaactgg
    14701 ctgacgctat gcgcttagca gaagaaaaga atttgatctt agcagaagcg atgacaatct
    14761 tcaacatgcc actttatcag cagttacgct caataatgga tacaggaaaa ctgggggctt
    14821 taaaaatgat ccaagcacct ttcggcagtt acaaagaacc agaccctaaa aaccggttct
    14881 tcaatccaga attagctgga ggagctctgt tggatatcgg tacgtatgct gtatctttcg
    14941 ctcgtttctt tttgagctct caacctgaag tagttgcctc tacaatggtt ccttttgaaa
    15001 caggcgtgga cgaacaatcc gtcactatct tgcgtaacaa agaaaatgaa cttgctgctg
    15061 tcagcttgac ttttcaggca aaaatgccaa aagtcggtat cgttgccttt gagaacggtt
    15121 atgtgacgat tgctgattac ccgcgtgctg atcgtgcgga gatcttattt acagatggta
    15181 caaaggaatt catcgaaagc gggaatacat ctgaagcaat gaattacgaa ataaagaata
    15241 tggtacacat gattgaaggt gacttgccca atcgctctct cttcttgaca aaagatgtca
    15301 tcgaaatctt ggatcaaatg caagttctat ggaaaaatat gtaattttaa aaggttgtga
    15361 aaaagctgtt ctgcatcaag taataagaac agccaaacaa aaatagctcc tcctattttt
    15421 cgtttggctg gacttattac cgcagaggaa agcttttgaa caccgtttaa gaaagaggtt
    15481 gtgagcctac cgttcagctc cgagtcacaa ggagcaattt caaaaaacag cttctcatgt
    15541 ttgttgaata ttgtgacttg tgaccgagga gttgacagga gaacaccgtt taaagaagag
    15601 gttgtgaaat cagctttttg acctgaatat tagctgaatt cacaacctct atttttggat
    15661 tttttgttgg gagaattctg ccttgatttc aataaaactt ttaaaagact gcaaaaactg
    15721 gaacaattct gctcgatcct cgaattcagg gcgactagtc ggcagaggca tttgccgata
    15781 caatacataa acttcttcaa tacgtgttaa aatttgtctg ccatcatttg actctgaaaa
    15841 tgtttctgca gtataaatca ataaaccata gatatgtttt ccataataag ctggttgctg
    15901 gattctctct agattttcca gcatgtcttt aatcacattg gcctgtgctc ttctcataga
    15961 gaagtaagtt tcgtagtata gcggctgtct gagccagtag ttttcttgat gttctctggc
    16021 atcttcttgg ctttcgcgaa tgaatgtcag taaatgttcg catttgatct gcacatcttc
    16081 tttattttct gataaaagaa attctgccat ttgtctcaaa atatttcgaa attctttttc
    16141 gatctgtatt tggttttcct tcagctgctt cgtacgatct ggcatgtaaa catttgcaag
    16201 caaagcaaat actaatccaa tcgccattaa tcccgcttca ttgccgatca gctgccaaga
    16261 catgttttcc tccactaaat aatgagtgac caaaaccgaa tttactacta tcccttcggt
    16321 taatttgaag cgagaggcaa gcgggatgaa caggaataaa aaaagactga agccaaaaat
    16381 agtataccca acagcaaaaa aactgaacag tgctgttgcc gtagctaata taaaggctgc
    16441 gattcgattc atgcccgtac gcaatgtaga gcgcttggta tttcctacac ttaataatgc
    16501 aatgatccct gcagctggcc aatacaacag cgataagcta cccgctgtga ccatcgaaaa
    16561 gaagacactg atcactgttt tgactgttcg cattcctata cgcatttact tccccctttt
    16621 ttattttaat tatacttgaa taatgaaaaa ctagctatca gacaaaagtt taggtatgat
    16681 agtaaaaaga ccaaaaagga gaatctctat gcaatttggc cgttttcgac taggtatgcg
    16741 tacgatcaaa agtgccttag ctgttttttt atgtatcttg ttttttcatt tgactcaacg
    16801 aggaatcccg atgattgctg cattatctgc tgttttttct ttgcgtcagg acttgactac
    16861 aaccgtttct tttggaaagt ctaggatcat cggcaataca ttaggtggta cattagctat
    16921 catttatttt tttgtcaaaa gctattttca caatgatttt ttggtagaat tgatcttgct
    16981 tcctattcta gttattattg tgatcgtcgt gtcagatggt attaacaata attccggtat
    17041 catttccgca atagctacgt tgttgctgat tgcgttgagc atccctcaag gcgaatccgc
    17101 tttgtatgct atccaacgtg tactagatac ttttataggg acattcattg caatcggtat
    17161 caatttcttt cttcgtcctc ctgaaacaga aaagaaggaa gaactatcag aagatttggt
    17221 cgaactccaa aagaaagaac gattgctaag aacaaattta caagagatag aagctaagat
    17281 ccaaaaacaa aagaaaaata gcgaggacta attttctttt gcaataaaat aaaaaaatgg
    17341 agagaagaac tttaaaaagt cttcctccat ttttgattga acggtgaagt gtactacttt
    17401 attcttcctt catcgagttt tgttctgcct cttttggcgc ttcatacata tttacttggc
    17461 tagtagtccc acgtgtcgac aaattctcta cgatttcctt caagtcaagt cctgttgtcg
    17521 cttttagtgt ttcttgagta gaagaaagca aatttgtggc ataatttgtc actcggttcg
    17581 ctccactggt ttctcctgaa ctgcttcctg tatcgacaac agagattttt tcgatatttc
    17641 ctagcggctg tgctgcttcg cgcatcaatt gcggtagcat atcgataacc atactcaaga
    17701 cagctgcttc gccatattcc ttgaaggcat tggcgatctt ttgttttgct tcggcttctg
    17761 cttttccttt tgctagtgct gcttcggctt ctgcttgtcc agctaaacgt gtcttattgg
    17821 cttccgcttc tgccagtgct tccacacgga atcgttctgc ttcggcttca gtcacttctt
    17881 tgactttttg agcttgtgct tcttgttctt tagcgtaccg atcggcatct gcttttttct
    17941 tcacttccga atcatattgc ttttcacgac gtgtgatttc tttttcttcc agttcgattt
    18001 gtttttgacg ttctacgatt ttaatctgca tttcctgttc gattacttgt tgttgtgctc
    18061 gggcactttc taaattgtag gcttggtctg cttttgcttt agcaacatct tgttcttgtt
    18121 tgtaggttgc taatttcagt tctttttctt ttaatgattc tgcgatctct gtttgtcgct
    18181 gtaattcagc tgcttgtgat tctttttctg cttgagcttt tttgatacgc gtttctttta
    18241 gagcttccgc ttctgctata tctgcatctc ttttgacttg ggcgattctt ggtttcccta
    18301 aagagtcaag ataaccgttt ttgtctctga cctctttgat agtgaaagaa acgatgatca
    18361 accccatttt tgcaaggtct acagaagcga cttcttgtac gctttgactg aacttatcgc
    18421 gattttgata gatttcttct acagtcatag aaccaagaat cgagcgcaag tgtccttcta
    18481 atacttctct tgcttcattt tccagttctt ctcttgtctt gcctaaaaac tgttctgctg
    18541 ctgtagcaat ctcttctaca gatgaaccga ttttgatgat cgaagtacca tcacacatca
    18601 ccggtacgcc ttgttccgta tacacttcag gggtggagac gtccaattta ctcgataata
    18661 gactgatccg attcgaacgt tgaaaaactg gcaagacgaa tgcgccacct ccacgcacaa
    18721 ttttgatttt gttattgctt tcatcaatgt gcacattttt gcttcctaaa taacttccac
    18781 tgatgatcaa cgcttcatcc ggttttgctg tctggtactt cgctacaaag acgatcagca
    18841 acatcagtaa aatcaaagct gcgattgcga tcgcgataaa aacaactggt atttccattt
    18901 aacacacgct cctttaagct tttatcatgc tttcataagg catgacataa cagattcttt
    18961 ccttgatctc aatgatcaat actttcgttc cccgctcagc tgtctgttct tgggggctgt
    19021 aaaaacaagc tggacgacta atggaaccgg taacactttt caactgaatt tctcccatac
    19081 ctcgtacagg gatcggtgtg atcacaacag ctacttgtcc ttccagagtt ttttcagaag
    19141 acgacatcgt tgattcggca tgtttcatcg gcatcaatat atagaaatta agcaaaaata
    19201 ccaacactat cgatatgcct ccactagcaa taaataaaaa taaagatgat tgatctgtta
    19261 aacgctcacc tagaaaacca aataagcttg tgaaagttaa ccaagggata ataagcatcg
    19321 gatcaagcgg gccatcaaaa tcgaatacat ctccaaaaat aatcaatagg actgccaatc
    19381 ctgcacaaat cagcaatgta taccaataaa tcgtctcaag tgcgattccc cctatcatat
    19441 acacgcctcc ttccatttct acgtctatca taccaatgtt tgaaaattct gtatagcttc
    19501 ttttaccgac tcagtggtgt tttttatagg aaggttgtgt ttatcgttga caatttgtta
    19561 aatttttgtt ttcatgttaa aattttcaca ataaattctt tcgaggtagc ctatgtttcc
    19621 tttttttaag aaaaaaccat taaagctcca gaacacgatc atccttgtcg tttgtttctc
    19681 cacattcgtt tctttgattg tttctgccat attgatcagg aattttattc tttcgatgaa
    19741 ccgatctagt gtgtcagata aaatctctag cattgctaaa atcgttgcca atgataaact
    19801 catccaaaaa aagctgcaga ctggtgaccc gacacatgcg attcaaggaa taacaaatga
    19861 aatcatgatt gcatcgaatt tagattttat cgttgtcatg gaccctgaag gcattcgata
    19921 ttctcacgca gacccaagtt acatcggtct tcctttctca agtcatgaag atgtgaaaaa
    19981 agtgattgga ggacaagaat ttttctctga aaaagaagga atgttagggt tcggcttccg
    20041 ctatttcgtc cctatttatt atgatcaaga gattattggt gcagtttgtg ttggcttaac
    20101 aaacaacacg attgacaatc aagtcaaaga tggacaaaaa atgattgttt tttcattgat
    20161 tattggactt agcgtaggca tcataggcgc cgtttttctt gcccaaaaaa tgaaaaaagt
    20221 actgcttgga cttgaaccac aagaaatagc agaacagtta agtgaaaaaa cgattatcga
    20281 aaatgaagta gatgaaggaa tcttagccat ctcttctgat aaaactatcc tcttgatcaa
    20341 tacacctgca aaaaacattt tctaccaaac agagcagcta ttggatttcg aagtaggaaa
    20401 aaaatcagtg ataatctata tgacgttttg tttaaagaga cgattgagca tcaaacgacc
    20461 gttcggactc aatccatgta tttaaatggg atagaaacta tttctagcgt tacacctctt
    20521 tttagcaatg accgtttttt aggagcagtc gcaactttac gaaatcgatc agaagtaatg
    20581 aaattaatgg aggagcttgg ggggacgaaa caatatataa actccatgag agctcaaacc
    20641 catgaattca tgaataaact tcatgtcatt tctggtttga ttgatttgaa gaagtatgaa
    20701 gaagtcaccc attttatcca gcagctaaat caaaattata aaaaagagat tggccaagtc
    20761 tcaagtctga tcaaagtacc tgcaattgcc ggttttttat taggcaaaat aaacgaagcc
    20821 aaagaacaat ctgttgaaat gctgttagaa agcgattctt ttatccctaa aatggagatg
    20881 gatgactcca tccattcact tttgcaagtg ttagggaact tacttgataa tgcaaaagaa
    20941 gccgtgctac aaaaacatga aagaaacgga caggtccgga ttcatttaaa ctatgacaca
    21001 gaagctgaag tattcatcgt gaccgtctgc gatgacggtg tcgggatatc agaagaaatc
    21061 tcagaaaaaa tatttacgct aggtttttcg acgaagggaa tcaatcgtgg gtttggattg
    21121 aatctcatcc attcaatcgt caaacgccac ggtggatttt tagaattttc caaacaaaca
    21181 aatggcggta caatcgtcta tctagaatta ccaaaaaagg aagatcaaaa tgatgacaaa
    21241 tgtcctgata atcgaagatg atccaatggt tgcagaattg aatcgtactt ttgtggaaag
    21301 catccctaat atgaacgtaa tcgaaagttt cagaaatgta gaagaaggaa aaaaatattt
    21361 cagacaaaaa aacgcagatc tgctgctgct tgatgtatac atgcctggcg aaagtggtat
    21421 ccatttttta tcttggctta gacagcaagg aaatgatatc ccagtgattt tgatcactgc
    21481 tgcagatgat atcgaaacca taaagaatgt gatccgctac ggtgccttag attatttgat
    21541 caaaccgttc actttgcaac gttttcagct ggcgatcgaa aagtatcttg ctttctctca
    21601 gacaatccaa acgactgaaa aagccgatca gaaattgatc gatcaagtat tcaaccacac
    21661 cactcccatt cacgaaatac catcattcat cgatttgcct aaaggtctgt caaaactgac
    21721 tttaaaaaaa ataagtgcat ttattctaaa agaagatact attttttcaa caaaagaact
    21781 ggctcaaaaa agctcactgt cacgtatatc cgtcaagaaa tatttgaatt ttttagtaga
    21841 acaagatata ttgaacgaaa gaatcaaata tttggaaatc ggccggccgc taacctgtta
    21901 tgaattgaca aagaatgggc ggaaaaagct cacagattac tgttgagtca tcgaattatc
    21961 ccatacaaaa tagtccaagt atacagaagt aacaactgtt tacttggact atttttaaac
    22021 attttattga cgaatatcca tgttttctta tcttaattga cgggctttag gaagagatac
    22081 ctcatttttc gtttttccag tttctattat ttcacgaaag ttctcgaaac ttgtcgaaat
    22141 catatttgat aatgcctcat ctgtatttga acctacatgc ggggttacaa gaactctagg
    22201 gtacatttct actaattgct ggatagcagg gtctggaatc gtctcccatg gcttgaagga
    22261 tcggaaaaaa acctctgttt catttgcaaa aacatcggtt gcaaaaccag ctaaatgatt
    22321 gcttttcaaa gctcttaaaa tagcttcatt atcttgaagc tctccacggg ctgtattaat
    22381 caagatgcta ccttttttca tttgagctaa aaatccttca ttgatcattt gatcgttttc
    22441 acctgggata tgaggtacat gaatactgac aatatcgctt ttggctaata actcttcaag
    22501 cgagtggaac gtcaatactt ttttagcagc ttctgtctga tgaatatcgt agccgatcac
    22561 tttggctcct agtcccttaa acaaaacagc ttccgtcaac cctatcctgc ctgttcctat
    22621 gatccctact gtgcaatttc tgatttcacg actaaacatt tgctcatcca ctcgaaaatc
    22681 tttgaaagat gttcggatcg tcgtatacgc agtatttctc aaaagcatca tggcaaaagt
    22741 caaagataac tctgctatcg catttggcga atacgatggt acacgggcta ctgtcatacc
    22801 aaaatcagct gccgcttgta gatcgatatg attgactccc acagtccgtg taaaaacata
    22861 acgaatccca tattcctgca tttttgcaag attggtctca tctgctatac aatttcccct
    22921 caacaataca gcatcatggt cctttgctga cacgatattg tcatgtgtca acagttcttc
    22981 ataaagactg agatcataat ggtaaatatt caatttttca aaaaaatcta tctcgttcgg
    23041 acgaacaccg taacatgcga ttctcatact ttcttcctcc atttcttatg aaaataaacc
    23101 tatgccatga atcatttgta atacaacgat ccatacaggt gtcagtacca cagcaaaaat
    23161 agttgacagt aaggatgcat tggaggctaa aagtgcctcc ctatcaaaac tgatagcata
    23221 ggctgctgcc actgttgcag ctggtgtagc catcatgata acgattgttg ccagtgcttc
    23281 aaaattgact ggtagaatat tcgttgccgt caaaactgct agaatcacga tattcaatgc
    23341 aggtacaaat accactttga ctagactgta ataccatgaa gttttttcag atgcagcttt
    23401 tttgaagctt acttcaccta atgttgcgcc gattgctagc cacgccaatg gtgaacttaa
    23461 accagctaaa taatccatcg gctgaaatag ccacggtgca gtctggtcaa ttcgcaaaaa
    23521 cgctacttgg cttatctctc cttctgcacc tgtaactgac acttgtggca gatacccttg
    23581 gaacagccaa atgaaaaggc cggcaaaagt agcgataatg attggattga cgaacatcgc
    23641 tttgatattt ttagcttcca tcttcaagcc actcattttt atgtatccat aagaatacaa
    23701 aaagattcgg tagccaatat tgaaaattga ggaatacatc acgccgacag gaccataaat
    23761 cgcactgacg ataggcgttc cgaaaaaggt cgtagaacca aagatcgtca ggacacgcaa
    23821 cgttgtttgc ttatcccctt tatagcttaa gaaaaaaggt tttgaaacta gaataagaat
    23881 aatataaata actaatcccc aaatcaagac attgattcct tgcatcagta attctgtatt
    23941 gatatcttgc atgaaagctc gaaatgccag tgctggcaaa gccactgtta agacgacttt
    24001 cgatagaatt tttccaacag attcagaaaa aatccccttt tttctacaaa caaaccctaa
    24061 taatataata aaaactgttg aagtgattgc actgacaatc gacatatctg tcaaagtcga
    24121 tcgaaaaatg tcaaataagt tcatctttct cctcctaatt atgttcattt attcacaata
    24181 aaaaaagctt ttattttgct aatacgtatt aacattttta attttatctt tacattgtta
    24241 cttattgaac aataggaaat taaagaaact tttgaaactt atgtaaagtg atttttacta
    24301 gaacacaaaa aaaaccttaa aaccaaactt aagaagttta gttttaaggt taacttaaat
    24361 tatattatgc gtggaaacgt tccacgccat ctagataagt tgcttccaat gtcatatcag
    24421 ggttcaatac gataaagtct gcatcgcgtc cttgagcgat ttttccacat gtatcatcga
    24481 ttttacagct gattgctgga atcagactag ccatcatgac tgcttgttct ggtgttgcta
    24541 atccccagtc tacaacattt ttaattgctt ctttcaattt caatatactt ccagctaagt
    24601 tgccagattc tagacgtgct gttccttctt ttactactac tggaaattcg cctaaattgt
    24661 aatttccatc aggcatccca ccggccatca tacagtctgt aattaatgcg acatgatcat
    24721 gtcctgcttt ttccattagg attcccgcag catttggatg tacatgatgg ccatcacaaa
    24781 tcaattcaga aaagacatgt tctaaagata ataacgcacc caccatacct ggttcgcgat
    24841 gattcaatcc acgcatacca ttgaaagcat ggacaaatac acttgcgcct gcttcaacag
    24901 ctttttgcgc ttcttctagt gtagcattac tgtgtcctag agaaacaact actccttctt
    24961 cagtcacttg tttgacaaac tcttctacac cttgacgttc cggtgctaaa gcaatttttt
    25021 tgattaaacc acctgaagct tcttgccatt catggaaagt atccaaatca ggatccccga
    25081 aataacttgg gttttgtgcc cctttgtgtt cttctgtgaa gaaaggacct tcaaaataga
    25141 ttcctttgat tttagctcct ggtgcttcct gatacatttt gccgattgtc tcagctacat
    25201 cttttaaacg ttctttgctt gacgtcaatg ttgttggtaa aaatgaagtc actccacaag
    25261 ataacagacc ttcagacatc actttgatgc cttctgcatc attatccatt acatcatgat
    25321 tcatatagcc gtgaatatgt gtatctacca aaccaggagc aatccatttg ccgctttggt
    25381 caatgatgtt gacttctccc tcaggtagtt ctttggtgta ttcaccaaaa agtccatcgg
    25441 taatttcaag atagcctgca tgtttgacac ctgacgctaa aaagaatttg tcagcataga
    25501 taaaagttct cattctattc tctccctttg tttttctacc tctaaagtac tcttatttag
    25561 agaagaagtc aagaatggtc tacaccattt acaaattttt tgaaatttgt ttcacagctt
    25621 gtgctaagcc ttcatagaat gctgctcttt ctggttcttc tttacgcttg tatattaagc
    25681 ttttaagcac aaataaatca ttgaggtaat agtagctatt cttctcttga gcccaataga
    25741 ttccttgatt cacctgttct aacgcttcgt cgatttcatt ttgatggaat aagaaatttc
    25801 cataattata aaaaatttgc gaaaattcac tttttgatcg ttcatcagct gattgatgca
    25861 gcagttggat gcttcgactc aaatagtagc gtgcttcagc cgtttttcct gccacaccat
    25921 atatttttcc gatacaacta atcagttgta tttctgtgtc agaaacatgg attctttctt
    25981 tttggctaga gtagcttaat gcttctttca aataataaag ggactgagag atattttgac
    26041 gcaacgtgaa ttcgcaaaca ccgagataat aataatagcg ttgaaagtca gttgcttgat
    26101 acagattttc agaaatatca gaagatttta gtacttgttc taacttccga taatcacgtt
    26161 tttggaaaca actatccaac agttcaaata atttatcgtt tgtccgagta gtttttcgag
    26221 agagattcat cacatgatca atcgttaccc ctagccgttg acaaatctgc atcatcacca
    26281 atacattcgg cagttcctca ttattttcga tccgacttaa tacactttgt gaacaaatat
    26341 catgcgaaag cattttttgc gtcatttttt gttcttttcg aatctcttta atgatcgttc
    26401 caaaggactc gattaacgtt tccataagta tcctccccaa aatatgcgta ttcgcattct
    26461 attcgtattt tactttagga agtcgcaaac ttcaagaaaa gacttgcaac taaataatat
    26521 actttagtta tttaagaaaa ctccactaga aagagagcct cagtgtgttt gtctgtggcc
    26581 ctcctactga aaaatttggt ttttaaaatc atttttggcg ataggttgct aggaattctc
    26641 tcattttctt agctgtttgt ttcaaatcat ccattttagg aagataaacg attcgaaaat
    26701 gatctggctg gttccaatta aatccaccac catgtacaag tagaatatga tgttcatgta
    26761 aaaagtccaa aacgaatttt tcatcatcca aaatattgaa tcgctttgta tcaatttttg
    26821 ggaatatata aaaagcagct tttggtttta cggctgaaag accaggaata tcattgattg
    26881 cattatagat atattctctt tgttcataga tccttccgcc tggcaggagc agctcatcga
    26941 cactttgata accgcctagc gcggtttgga taatttgctg agacaataca ttggaacaca
    27001 aacgcatgga ggacaacata ttcagccctt ctatatagcc tttgacgtgt gacttatctc
    27061 cacttaggac catccagcct actcgaaatc ctgctacacg atgagatttg gatagaccgt
    27121 tcagcgtaac aacgaacaaa tcaggcgcta atgtagcaat cggaatatgt tctagaccat
    27181 ccatcactaa gcgatcatag atttcgtcgg aataaataat caggttattt tgtcgagcta
    27241 cttcaacgat ttcttctaat aattcttttg gataaagtgc cccagtagga ttattaggat
    27301 taatgataac aatagctttt gtccgactcg ttatttttga tttgatatca tcaatatcag
    27361 gataccattc tgcctcttca tcgcaaatat aatgaacagg tgtgcctcca gctaatgcaa
    27421 tagaagccgt ccacaacgga tagtccggca ttggaaccag tacttcatct ccattgtcca
    27481 gcaacccctg catacacatc gtgatcaatt cgctcactcc gttgccggtg tagatatcat
    27541 tgatcgtcac attcggaaaa tgtttcagtt ggcaatattg ctcaattgct tttctagcag
    27601 aaaagatccc cttggaatct gagtagcctt ctgagtcacg ggcattcatg atcaaatcac
    27661 gaatgacttc gtttggtgct tcaaatccaa aaggtgcggg atttcccgtg ttcaatttta
    27721 aaatactaat gccctcttct tgcatccgct ccgcttcttc tagaacggga ccgcgaacat
    27781 cgtaactgac accttctaat ttgttggatt tttcaaattc tctcatcact tagccaatcc
    27841 ctcatttcaa aaagttagaa ctttccaaat tttttgttat ttactatttc aatatcatac
    27901 tccatcctag aaaattttgc cacttattta ggaaaaacgt aagtattaaa aaaacacaaa
    27961 caagcgttga gaacttaatt gtcatgatag tcattccata gattcgctgc ccaatccatt
    28021 gcaattccac aaatgattcc tagcataaaa ccaagcaaaa aacgtccgaa tgcaagtcca
    28081 atcaatacac ctaagatcat tcccagtcct gaaaattctc cgtcatattt ctccatctta
    28141 ataagtcctc ccatttttga gcttgacaac tatgtaaaac ctcgctatca ttagtgtgtt
    28201 attattacgc agatgtgttg gaattggcag acaagcatga ttgagggtca tgtgagcgtt
    28261 agctcgtgtg ggttcgagtc ccatcatctg cactacaact cgtgaagtta attttgcttc
    28321 acgagttttt ttgtttgaac gtttctttat gccattatat acatatcact gtatccttta
    28381 caaaaagaac gtacaatcat gtaaacagcc tgaggtgagt atatgaaggt cggaaacaag
    28441 ctactcgtag aaacttgcaa gtgttaaaac aattaatcga aaaacgagta aaatccctca
    28501 cctaatacta aagcgaggga ttttttgtat attatctgtt tatcaccgtt attataaagg
    28561 tacaaatacc aagcacggtt aatccaaatt gcataaattc atttttttgt acactcatgc
    28621 ttttcattag ctctttaact gccattagta ctaagatggc gataacaaca taaagtatat
    28681 agtacgtccg cattccggaa gcccatactg tgggagaaaa tcccataatt aaccttgaac
    28741 aaaatcccat tatcatcaaa ataactaaaa aaatagaagt tttcttattg tcaaaactta
    28801 aataaattcc aacaattaaa catagtaata atgccaagac tactaaagta gctatccaag
    28861 tacctggata aaaaagtgac agtttcgttc ctaactctgt aaagagatta ttcaggttac
    28921 tactactcca aatcattgcc cttttatttc cattgacgta agtaaagctt tgacccattg
    28981 tattaccaaa ataaataatc aggtttaaga aaaacggtaa tgcagtcaat atacgcacat
    29041 aatagttttg acatttccta tatgttagaa ggaaaattaa gaaaaatagc aataaaaata
    29101 agatattcat gtctaaaaaa aacggctttc caaatgatga aatacctaaa tctaacttat
    29161 taagaaagtt gaaattttta tattcaggaa accatttatt aatttcatgt gcagcacgta
    29221 atgcattccc tggagttgtc aaagagaaaa ttaattctgc taagcttatt attgaaaata
    29281 ctgataactt ataattatat cgtccattga caatcaaata taaactaact atacttgtta
    29341 gaacaaaaaa acaggcgttg acttgttctt gattagttaa aaatatcaat aaaggaatac
    29401 ttacccaata tatttttcta tttatttctt ctcctcttaa gagttgaaaa aacggataaa
    29461 aagttagtaa actaaatgct actggccact gatagttcaa ggtggttgct ctccaaccgg
    29521 tttcccccat tactgtcaat ggtattaaac agtatataga gcaaaccaat aataaatttt
    29581 taacatttat tttctcaaaa acata
//
LOCUS       DACCKU010000028        29108 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.030, whole genome shotgun sequence.
ACCESSION   DACCKU010000028 DACCKU010000000
VERSION     DACCKU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.030"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1029)
                     /locus_tag="IVN13_001170"
     CDS             complement(<1..1029)
                     /locus_tag="IVN13_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7011669.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1111..1800)
                     /locus_tag="IVN13_001171"
     CDS             complement(1111..1800)
                     /locus_tag="IVN13_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP7011670.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IVN13_001172"
     CDS             complement(1906..2274)
                     /gene="rplL"
                     /locus_tag="IVN13_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP7011671.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2327..2830)
                     /locus_tag="IVN13_001173"
     CDS             complement(2327..2830)
                     /locus_tag="IVN13_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP7011672.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDAVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IVN13_001174"
     CDS             complement(3240..3929)
                     /gene="rplA"
                     /locus_tag="IVN13_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP7011673.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IVN13_001175"
     CDS             complement(4033..4455)
                     /gene="rplK"
                     /locus_tag="IVN13_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP7011674.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4798..5466
                     /gene="sdaAB"
                     /locus_tag="IVN13_001176"
     CDS             4798..5466
                     /gene="sdaAB"
                     /locus_tag="IVN13_001176"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP7011675.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5479..6351
                     /gene="sdaAA"
                     /locus_tag="IVN13_001177"
     CDS             5479..6351
                     /gene="sdaAA"
                     /locus_tag="IVN13_001177"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP7011676.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6442..7461)
                     /locus_tag="IVN13_001178"
     CDS             complement(6442..7461)
                     /locus_tag="IVN13_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7011677.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7634..8521)
                     /locus_tag="IVN13_001179"
     CDS             complement(7634..8521)
                     /locus_tag="IVN13_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7011678.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IVN13_001180"
     CDS             complement(8598..9143)
                     /gene="nusG"
                     /locus_tag="IVN13_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP7011679.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IVN13_001181"
     CDS             complement(9244..9414)
                     /gene="secE"
                     /locus_tag="IVN13_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP7011680.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IVN13_001182"
     CDS             complement(9438..9590)
                     /gene="rpmG"
                     /locus_tag="IVN13_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7011681.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9752..10396
                     /locus_tag="IVN13_001183"
     CDS             9752..10396
                     /locus_tag="IVN13_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7011682.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10452..10955)
                     /locus_tag="IVN13_001184"
     CDS             complement(10452..10955)
                     /locus_tag="IVN13_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP7011683.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10968..11014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11552..12517
                     /locus_tag="IVN13_001185"
     CDS             11552..12517
                     /locus_tag="IVN13_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP7011684.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12616..13788)
                     /locus_tag="IVN13_001186"
     CDS             complement(12616..13788)
                     /locus_tag="IVN13_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011685.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13775..14920)
                     /locus_tag="IVN13_001187"
     CDS             complement(13775..14920)
                     /locus_tag="IVN13_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011686.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14934..15866)
                     /locus_tag="IVN13_001188"
     CDS             complement(14934..15866)
                     /locus_tag="IVN13_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011687.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15983..17062
                     /locus_tag="IVN13_001189"
     CDS             15983..17062
                     /locus_tag="IVN13_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7011688.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17059..17688
                     /locus_tag="IVN13_001190"
     CDS             17059..17688
                     /locus_tag="IVN13_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011689.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17798..18025)
                     /locus_tag="IVN13_001191"
     CDS             complement(17798..18025)
                     /locus_tag="IVN13_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7011690.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18128..20716)
                     /locus_tag="IVN13_001192"
     CDS             complement(18128..20716)
                     /locus_tag="IVN13_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP7011691.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20736..21395)
                     /locus_tag="IVN13_001193"
     CDS             complement(20736..21395)
                     /locus_tag="IVN13_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7011692.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IVN13_001194"
     CDS             complement(21444..21947)
                     /gene="trmL"
                     /locus_tag="IVN13_001194"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP7011693.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22168..23034)
                     /locus_tag="IVN13_001195"
     CDS             complement(22168..23034)
                     /locus_tag="IVN13_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7011694.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23143..23784
                     /locus_tag="IVN13_001196"
     CDS             23143..23784
                     /locus_tag="IVN13_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP7011695.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23846..25207)
                     /gene="mgtE"
                     /locus_tag="IVN13_001197"
     CDS             complement(23846..25207)
                     /gene="mgtE"
                     /locus_tag="IVN13_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP7011696.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25225..26124)
                     /locus_tag="IVN13_001198"
     CDS             complement(25225..26124)
                     /locus_tag="IVN13_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7011697.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26126..26923)
                     /locus_tag="IVN13_001199"
     CDS             complement(26126..26923)
                     /locus_tag="IVN13_001199"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP7011698.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26901..27605)
                     /locus_tag="IVN13_001200"
     CDS             complement(26901..27605)
                     /locus_tag="IVN13_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP7011699.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27736..28311
                     /locus_tag="IVN13_001201"
     CDS             27736..28311
                     /locus_tag="IVN13_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP7011700.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28429..29106
                     /locus_tag="IVN13_001202"
     CDS             28429..29106
                     /locus_tag="IVN13_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP7011701.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6169 c         5233 g         9143 t
ORIGIN      
        1 tacagtatta tttcggttgc ttgctgcttc acctagccct tgtactcgtg tttgattggc
       61 gacttgacca aattgattcc cgacgtctaa taaaataata gctaaaagca aaatggccaa
      121 atggcttccg ccaaccaata aagcaataaa gcttattgtt tgcataacaa gtcccatata
      181 cacgattttt ctttcagaat aagaatctgc cagtcgtccc acgataggtg cagcaactgc
      241 tccagacaaa ccaaaaatcg ctaatatccc tgcttcgaat gttccccaat tgtaggcggg
      301 actgctgata taaaaaatca acgtagacca aaagatcgaa aatgttccaa acatgaaaaa
      361 tccactgact gccgcttctc ttaatagacg ttgagaagca actaaatgcg gcaaactttt
      421 aagtgaatca aggtatgaaa cagctgtact actttgctgg acagaacgcg gcattttcca
      481 gtaaagcaga agtgtaagaa ttccgactgc acataaagca aataaataga tcatccgcca
      541 agaaaaaaga ctggcaacaa taccagatac cgtccgagaa agcaaaatcc ccacgagtaa
      601 accgctaagc aatgtaccca tgaccttccc tcttgctttt ggtcccgcta gtaccgcacc
      661 gtaaggaatg atgatttgcg gcacgatcga aagtaatcct attaaaaaag aagcgcatgc
      721 aaataacgta aacgaaggcg caaaaaaagc ggccaacaga gagacggcag ataacgctac
      781 cattcgaatg atcaattttg gacgatccac cacgtcccct aaaggaacta aaaacagtaa
      841 ccctaacgca tagcctaatt gggtcaacat cgtcaatatc ccgactgcac ttgtgctaac
      901 agataaacta gctgctactt ttgttccaat cggctggatg taatacatat tggcaacaac
      961 aacgccacaa gtgattgata atagtaaaat aagcgaagtt gttaaaggtg ttttttcatt
     1021 actcttcatt gatttccccc atttcctatc ttagtttaca acttatggaa aagattggaa
     1081 atagaaaaat acaactattc gccacactta ttatttgtgc ttttgttttc cttgtttttt
     1141 cttgcgatct ttataaatga agacaactaa ccctactagc acagtaacac ttgcaaccaa
     1201 cgtgactgca gttttgttat atccgtgaaa cagagcttct ccaccacacg catacaacac
     1261 agacgaaggg atgactccaa tcacgatcga caatatgatt tgctgtagag gcaatttcat
     1321 cgcatctgca gcaaaattga ccatagaaga tggaataacc ggtatcatat agcctaacat
     1381 tacacctatt tttggatgtt tcatttgtcg gattgcttgc acccaatgat tcgtttcggt
     1441 tttcttatct aaaaatttta agcgatgcat aaggaaaatt gcagtgatgt taccaagagc
     1501 attacccaaa atgttgatga tcgcacctgt cagtggtcca aagctcaaac cagcaatgac
     1561 tccaactact gaagtaggaa tacctggaac agcacacatc accgctgtca atacgatcaa
     1621 gagtatcgat gcaaaagtcc catgcgaacg aatatgtgcg agtagctctg ccttactcgc
     1681 tctcggattt aaaaataatt ggatgtctgt ctgatattct aaatacaacc gatagcccac
     1741 taccagtatc agaatgattc ccaacgagat caacatcaat cggattattt tctttttcat
     1801 atttcctcct gaaaaaaatc tatattcatt aaataatagt ttaacataaa aacaaaaaag
     1861 caccgaaatc caacatagga tctcagtgct tttaaatagt aaaaattatt taacagttac
     1921 agatgcgcca acttcttcaa gtttagcttt caattcttca gcttcttctt tagatacgcc
     1981 ttctttaact ggtgcaggag cgccatcaac tacagcttta gcttctttca agcctaaacc
     2041 agttgcttca cgaactgctt tgataacttt aactttttgg tctccagctg cagttaattc
     2101 tacagtgaat tctgtttgtt cttcagcagc agcagcgcca ccagcagcag ctacagctac
     2161 aggagcagca gcagatacgc caaattcttc ttcaatagct ttaactaggt cgttaagttc
     2221 taggatagtt gcttctttta actcagcaac gatgttttca atgttcaatg ccattataat
     2281 ttcctccgtt taattgttct tttttattta atgctttatg cggcaattaa gctgcatctc
     2341 catcttcttt gtctgccact gctttgacag cgtaagccac gttgcggact ggcgcttgta
     2401 gtacagatag caacatagat agaagtcctt cgcgatttgg caattttgcc aatgctgtga
     2461 tttcttccaa tgaagagact ttaccttcga tgataccgcc tttgatttct aatgcttttg
     2521 cttctttagc aaattcgtcc atgattttcg ctggtgcgac tgcatcttcg ttactgaatg
     2581 caacagctgt aggtccagtg aatacttcgt ccatgccttc aaggccagct tttttagctg
     2641 cacgtgaaag aatagaattt ttgataactt tcatttctac gttagcatca cgaagttgtt
     2701 tacgtaaacg tgttacttca tcaactgtta atccgcggta gtcaacgact actactgatg
     2761 cagctgctgt aaatttttcg cttacttcat caacgattgc tgcttttttt gcgataattg
     2821 cttcactcat ttttgagttt cacctcctga attttgatgg aagagttttt tgcactgaaa
     2881 aggcaagtca cttttatagt tactttgctc aaccagtatt attcatcacc tattcacttc
     2941 gttcatagtg attctactcg ctttgctcga aaggaaactc ctttcgcttt gctcaaccag
     3001 tattgttcat catctcttca cttcgttcat agtgattcac aataaaaaac tctatgccac
     3061 cgcagacata gagggactat tgaatatatt caataaacgt cctcggtagg aaattaaggc
     3121 ttgatgccac ctactgtctt cggtacaagt taattattaa cctcaactag cttagcatgc
     3181 taaactagtt gagtcaagaa ttatttttca gttggatcaa tcaacattct aatatttaat
     3241 tagaaagaag cttggtcaac atggatacca ggtccaaatg ttgttgtgat cgttacattt
     3301 ttcatgtatt gacctttagc tgctgatggt ttagctttta gcaatacatc atggattgct
     3361 gcaaagtttt caactaattt ttcatcgtca aatgaaactt ttccgattgg tacatggata
     3421 tttccagctt tgtccacacg gtaagttact ttaccagctt tcacttcgtt tactgctttt
     3481 gttacgtcca ttgtaactgt accagtttta gggtttggca ttaagccttt tggtcctaat
     3541 actcgtccta gacgacctac agtagccatc atatcaggtg ttgcaacaac tacatcaaag
     3601 tcaaaccatc cgttttggat tttttgaacc atgtcgtcgt cacctacgaa atctgcacca
     3661 gctgcttcag cttcttttgc tttgtctcct ttagcaaaaa ctaaaacagt ttgtgtttta
     3721 ccagttccgt ttggtaatac aacagcacca cggatttgtt ggtctgcttt tttagggtct
     3781 acgttcaaac ggtatgcaac ttcaactgtt gcatcaaatt ttgcaatgtt tgtttctttt
     3841 gctaaagcta ctgcttcggc aacaggataa actttacttg aatcaacttt ttttaatgca
     3901 tcttgcattt ttttgctctt tttagccatt ttggttcctc cttgattgtg gttataacgg
     3961 ttgtacctcc cacgtgttct atatctttca atatagagcc aactgagaag ctgctgtctt
     4021 agggtgtata gattattcta cagtgatccc catgcttcgt gcagttcctt caaccatgcg
     4081 cattgctgct tcaacgttag ctgcgtttag gtcttccatt tttaattcag cgatttctct
     4141 tacttgatcg ctagaaactt tcgcaacttt atttttgttt ggttcgcctg aacctttttc
     4201 gattttagct gcttttttca atagaacagc agctggcggt gtttttgtaa cgaatgtgaa
     4261 tgaacggtct tcgtatacag aaataacaac cggaatgatc aaacctgctt gatcagctgt
     4321 acgagcgttg aattcttttg tgaatcccat gatattgata cccgcttgac ctagtgcagg
     4381 acctactggt ggagctggag ttgctttacc tgcaggaatt tgcaatttaa cgattttttc
     4441 tactttcttt gccacgagac atacctcctt aagtccgtga tgtggttaat tggagatgca
     4501 tatttctcct cccacgtttt atctgatctg aaaaaatcca gacaaataat gtgcaaaaca
     4561 atgcacactg gtacagtata gcaaatttat ttgttttttc aagatttttt tctgctattt
     4621 ttacattttg cttgaataaa cgctaaattt attctaaaaa aacaacaatt tttcgttctc
     4681 atttcttctt ttatgttaca cttgcgctag catggttcaa cagatgaaag caaacaataa
     4741 cacggttttt tagacccttg tacaaagatt cctacataac aaaatgaggt ggagaaaatg
     4801 aaagatttaa ggtatagaag tgtctttgat attattggac ctgtgatgat tggtccaagt
     4861 agctctcaca ctgccggagc tgctagaatc gggaaaatca tacgtagtat atttggcgaa
     4921 cagcctgatt ctgtagatat ttatctatat gaatcatttg caaaaaccta tcgggggcac
     4981 ggaacagata ttgcattagt tggcggcctg ttagggatgg aacccgatga tgaacgttta
     5041 gctgattctt tgaaattggc atatgaagcc ggtatggaag tcttgtttgt tcctaagagt
     5101 gaaaaagccg aacatccaaa ctctgtaaag atgcttctaa aaaaaggaac acgaaagctt
     5161 tcagtcactg gcatttcgat cggcggtggc aatatccaga tttctgaatt gaatggattc
     5221 aaaatttctt taagcatggg tactcctacc ttgatcgttg tccaccaaga tgttccaggt
     5281 atgattgcta aagtgactag tatactttct gagaccaata cgaatatcgg cactatgaca
     5341 gtgacacggg aatcaaaagg tgaaaatgcc attatgataa ttgaagtaga tgatcctcaa
     5401 gtagaagaaa cggtaaaaag actcaaacag ctgccaaata tcgacagcgt aaattatttc
     5461 gattaaggag ctaaaaaaat gtttttatcg attgaagaat tagtagatca agcaaaagct
     5521 tatccaagtg tagcagaatt gatgattgca gttgaaatgg aaatgagtgg ccgttcccgt
     5581 agtcacatca tcgagatcat ggaaaaaaac ctctcagtaa tggaacaatc aatcaacgag
     5641 ggaaaagaag gtgttacctc cgtcacagga attaccggtg gtgatgcaaa aaaattagat
     5701 gattatttaa atgaggcaga ctttttaagt ggagaaacaa tcttaaaagc tgtacgcaat
     5761 gctgtagctg ttaatgaagt caatgcaaaa atggggttga tttgtgctac accgactgcc
     5821 ggaagcgcgg gtgttgttcc tggtgtgctg atggctgtcc gagatcggct tgatttgaca
     5881 catgaacagc aattggaatt cttatttacc gctggcgctt tcgggttagt catcgcaaac
     5941 aacgcttcta tcagtggtgc agaaggggga tgtcaagcag aagttggttc tgcaagtgca
     6001 atggcagcag cagctttggt ttgtgcaaaa aatgggactc ctcatcaagc agcacaagca
     6061 gtagccatta cattgaaaaa tatgatggga ctgatttgcg atcccgttgc aggcttagtt
     6121 gaagtcccgt gtgtcaaacg taatgcttta ggctcttctc aagcttttat ctctgctgat
     6181 atggcattag cgggtatcga aagtgtcatt ccgccggatg aagtcgttgc tgcaatgtac
     6241 caagtcggac gtcaaatgcc gtcgattttc aaagaaacag cagaaggtgg cttagctgtt
     6301 acgccaactg ctaaacgact tgcggaagag attttaaata agagggcgtg ataaagctcc
     6361 ttaatttcga gtattaagag attagttatc ttcataacaa aaaacgaaga gaggactaat
     6421 tgcccttctc ttcgtttttt attaatccat caatttcgta ttttttactt catcgatcgt
     6481 ttttgttcct gcaagttgca tcgtgatcga caattctttg ttcaggtgtt caaaaacaga
     6541 tgttactcct tctgccccac ctagattcaa cccataaatc actggtcggc caatcgcaac
     6601 taagtcagca ccacttgcca gagctttgaa gacatgctct cctcgacgga caccagagtc
     6661 gaaaatcact ggtacacgtt tgttgactac ttcggcaatt tttggcaata cttcaaagga
     6721 cgcaggacct ccatctaatt gacgtccacc atgattggat acccagatac catctgcccc
     6781 tgcagagata gcgattactg catcttctgg cgattgtatt ccttttacga taactggaag
     6841 atgggtgatt tctttgattg ttttgatgtc atctggtgtc aagccttgtt ttgcagctgc
     6901 ataaatttct gcgattcctt ttccttcgcc attaccactt gcactttgtt cgctatacgc
     6961 agctaagtta ggcattggta atggaaattg gaattgattg atgacgtctt cttcacggta
     7021 accaccgagc gttgaatctg ccgttaaaat gatagcttta gcccctgctt ctactgcttt
     7081 gttcaaaatg aactcattga atctgtcgtc tttactcata tataattgga aaaactgcgg
     7141 tgcgcctggc gctgcatcgg ctgcgtcttt gattgtcgta tttgcataag tgctgatgga
     7201 aaagattgat ccagcagcag ctactccttt agctgtatca gcttcccctt tttcatgtgc
     7261 caatccttgg gccgctgatg gcgcttggat aatcggtgta tctaattcaa ttccgaaaac
     7321 agatgtatgc aaatcggctg agtcgattcc gcgtaatatc cgtggcatga ttttctttgt
     7381 attaaaagaa gtcgtatttt ctttcatcgt ccactcatct tcagaaccac cgcgaatata
     7441 gccaaaagct cctgcttcca tgcgctcttt tacacgacct tctagttagg caatgtttac
     7501 aatatcgatt ggatgttctt cattacttgt atgataagtc atagtagatc cttctttcat
     7561 cttcttgatt acatataaat catactaaac ttacaaaaag taagcaaaca tttactctca
     7621 gatactttta tcgctattct ttccaaataa atcgacttac aagctgatct acttcttggt
     7681 tttcatgaag catactatga gaggcatttt cacctgtcac tactttttcc tctatttcat
     7741 ttccgcgttg cttcaacaac gcatagaccg ctaatgcact gtttaacggt actgttccat
     7801 cactcaagtc tgtttcatct agttgaccag caatcaagaa aaatcgagtg gttataggaa
     7861 tattaccgat catttgctgg taatcttgat agcggttaga aacgacagca ggcccctttg
     7921 ctaactcatc tgtcagagac tgcgcactat catcgacaaa atcattaaaa ggcgcgccaa
     7981 ttgctgttaa tgtctgaact tgtggcaatg acgtatcctg tccatacgtt cctaaatagc
     8041 gtagtgcact aactcctccc atggaatgcc ccactatgtt gactttatct atgccgtagt
     8101 tttctttcag atacaaaaga caagttttga tccattctgc ttgattccac tcgttatttt
     8161 tattatcttc aaacaatact tggaccatcg gattgtctgc ttttccagac aaatctcctg
     8221 ttgcttgtat ttgtccatct gttcctactg ttaaaaccaa ttcttttact gcttgctgat
     8281 ttttttccaa acgccgcaac atccctttaa aggaattttc tgtcccgctg tagccgtgga
     8341 aaaaaagtgt tggaatagtg gttttttcta tcactttact ggattctgcc gttttttctt
     8401 tatcagaaac ttcagtagta tttatcttcg tcgtctggca accacttaac gcaaacaaac
     8461 tgataaggac taaagctgct gcagttgcac gtttgcataa atttttgatt ggttctgtca
     8521 tacacctctt cctttccata tagaaaaaag aaagacttga aaggccaatc atatgatcga
     8581 catttcaagt ctctttatta atcgatttta tctacttgat cgaaatcaag ttctgtactg
     8641 gtttcacgtc caaacatatc gatgttgact ttcaattttt ctttttctcc gtcaacttct
     8701 gttacttgtc cttctaatcc agcaaatgcg ccttcgatga ttttcacgac ttcgccaact
     8761 tctacttcta gatcattttg tcgcaggctc ataccgatag aacgcaagat atgattgact
     8821 tcttctggaa gtaatggtgc tggtttgctt ccggctccgt gtgaaccaac gaatcctgtc
     8881 acaccaggtg tgttgcggac aacataccat gaagcgtctg tcatgatcat ttcaacaagt
     8941 acgtatcctg ggaatgtttt atgaacgatt tctttttctt tcccattttt gacttcagtt
     9001 tctttttctt caggaacaac gacacggaaa ataaagtcgg ccattttcat actttgcgca
     9061 cgagattcga tatttgcttt tactttgttt tcatatcctg aatatgtatg caagacatac
     9121 cagttttttt caaaagattc cattgaaggc tcctcatttc attgtaaaat aaaaaaacct
     9181 cagtacaccc gaagtttttc tttcgcttca ttatagcact aaaagtacgt tgtggctagc
     9241 gagttatttt aaaatccagc caaataagct ttggatggct gtatccatta cgtaaaacat
     9301 caatccgaaa ataatagaag tttcgatgac tactaacgta tctttacgga gttgttttct
     9361 gcttggccaa gttacttgtt tgatttcatc ttttacgctt tttagaaatt tcatgagaca
     9421 acctcccttg ctactgttta tttcgtttct ttatgtaatg tatattgatt acaatattta
     9481 caaaacttat tgatttccaa acgttcgccg ctttttcctt cactgactga ttttgtgtag
     9541 ttgcgagacc cacaaacgga acaagcgagt gctgcttttt tctttgccat ttacttctgc
     9601 ccccttgctg agaatcagac ttatacttca taacagtatc acgtaacttt ttacttgtca
     9661 atttgtgaaa taaactatct attgttttgg ataaaattat gatatgatta atttgaaatt
     9721 aagctaaaat gagacaattg gaggtatagc gatgctatta aaaacacttg tatataagaa
     9781 aaaagatctg acaaccgtta cagagactag tacattagaa gaagcattaa aaattttaga
     9841 aacttccgga taccgttgtg tgccgatctt agattcttca ggtaatattt tccgtggaaa
     9901 catttacaaa atgcatatct atcgtcataa agcaaatggc ggtgatatga cgcttcctgt
     9961 cactcatctt ttgaaaaacg ctacaaagtt catttacctg gatacttctt tcttcaaagt
    10021 attctttacg atcaaagagc ttccttatat tgctgtcctt gacagtgaaa atcattttta
    10081 tggtattttg acacatagta cgttgcttaa tatgctctct caatcttgga gcgtagaaca
    10141 aggaagctat gtactgacaa ttgcttccac aggaaagcaa ggagatttgg ctgctattgc
    10201 aaaaatcatt tcaaaacaca gcagcattgc cagctgtatt actcttgata tcgaaaaaga
    10261 cgaatacatt cgacgtacat tgatcacgct accggctaac actgaagcga aagtatgtga
    10321 aacgatcgtc aaccacttag agaaaaagaa ttataaagtg gtagaaattg aaaatttgca
    10381 aaaccaagaa gcataatcaa aaagcgaaaa aagaaaacag ctaatctgct ttcttttttc
    10441 gctttttata attagacacg cttttcaaaa tcaagcgcgc gatctaaata atacatcaat
    10501 ggtgctgaaa cagccatgac gataaactca cttaatgcaa gcgtgccata ggttgtccaa
    10561 aatgcttggc cacctgttgg aactaacata taagcaatca gtcccatgct gatggtaaag
    10621 aagatcgtgt tcaatactaa acgcatcttt acatttttca ctttgttttg caataaagct
    10681 gtcaggccta aagaaagcaa tgtttggccg ccaccaaaaa ccacatccat gataccaaaa
    10741 ccaaagaagg cattatagac gatgaccccg cctaaaactc cccaaagcaa tttacggtta
    10801 aagacaacta aatgattcaa actttctgaa atacgaaact ggatcggccc tgaggataca
    10861 ggcgcaacta aaatagttaa cgctagataa agagccatga tgatgccatt tacaacgatt
    10921 acggataacc gttgtctgct tttcgttttt tgcattttct ctcctctttt ctcctagttt
    10981 ttttaacgcg ggatggttga tgaaccgcgg ttatgtgaaa caaacaataa ttataacaga
    11041 cttttcattt atttcaatcc gtcttatctg cttttaaaaa agcaaaagct taaacaatgt
    11101 aaacgtttgc taaaaatcgt gggatatggt ataaagtaag tgtaaggggt cataagaatt
    11161 cccagtaaag cagacgaagt aggatctgca agaacagaat tgttgtgaaa cttaaataaa
    11221 taaaaaagga tgtgggaatt tgagcaacct cttactagag aacggtctag caagcagctg
    11281 aagatctaag tggacatgcc gaaccttgga acttctcctt acgtaagtaa tggatcatgc
    11341 gcttgctagg cttttttgtg tttttcggtc ttcggacctt tttcattttc ctagacttct
    11401 gctataataa ccaagaatta attttgtaac agattttttt gaaaaaacaa gtttctttca
    11461 ttcaaaaaat agaaaaagct tgctaattat tggttaacca gcaaacagga gtattaaaat
    11521 atcttgtttt attgaatagc aggaggaaat catgaaattc cattatcata tattgatcaa
    11581 tccatcggca ggcagcggaa atggctataa agtagcagaa agaattttac cggtattaaa
    11641 aaataagcat attgactata cacttcatta tagtgaatac aaaggacacg atgctgagat
    11701 tgctgaaact ttggcaaaag aaacattgat tccttggata gaagaagaac agaaaacaat
    11761 cgatactttc ccccttttga tcatcgtagg aggtgacggc accttacatc aggtacttga
    11821 tactttctat cagatggaag tagaattccc agttgcttat attccagcag gttctggaaa
    11881 tgattttgct cgaggggcag gccttccaaa aaatcccaaa aaaggacttc agctgatttt
    11941 agcagctcaa tcaccagaaa aagtccacat aatggcttat gaagaaaaaa taagtgaaaa
    12001 aaaaggaata gctgttaata atttcggtat aggattagat gcagctatcg tccatgcgac
    12061 aaatcattct tccacaaaaa agcgattgaa caaatacaac ttaggatcat tttcgtattt
    12121 attctctatt ctccgtgctt tattcactca aaaaggattt ccaattctgg tagatgcagg
    12181 aggaaaacaa ttaagttttt caaacgcatt tttatgtaca gcaacgaagc atcctttttt
    12241 tggcggagga atcgcgattg ttccaactgc tgatgcgtct aaaccagtca ttgattttgt
    12301 cgtggtcgaa cgaatcaatt tctttaaaat cttgtggctc attcttttac ttatacagaa
    12361 aaaacaattg aaatcgaaat atttccatca ctacacaaca aacagattac gtatcgtttc
    12421 aacaacaccg caacatggtc aagaagatgg agaagaaatg gaaaaacgac cttttgatat
    12481 cacaataacc acaactaaac agttgttttg gtgttaataa aagaggtttt gtcagaagct
    12541 acatctggaa caccgtttat tcggaaaaaa ggactgtgac acgacttttg tcacagtcct
    12601 ttttttacta tgcagttaat tctttgccca attgggttgc catcgatagt ttctttcttt
    12661 acccaaaata aattggatca ttgaaaacac cgcagcaaaa gcaactaaga cacaaccatt
    12721 gaaccaaaaa gattctgcaa atgtttgatt gtaactgagt gtttcactga tcaagttatt
    12781 attttgaaca aaccaatatg ttggtgtgaa tgccgctatt ttgctaacca tttctggcaa
    12841 gtattctgct ggtacaaaca ccccaccgat aaagcaactg cctgtgataa aaatatcttt
    12901 gatcccaccg ctggtttctg agttttttac caatgaagtg atacaagccg aaaagctaat
    12961 gatcggcaat aaaaacaaga aagtatttaa aatatgatac cataccgtta tatcccacga
    13021 ggtgtggatc actaacagaa ccattagaag taagaagcca ctgaacaaca gcagcgagta
    13081 actaatcaaa ctaaaggtta ctttgcgatt caagtttcgt ttggtaatgg gcgcactctg
    13141 gtttcgttta ctgatctctt tgcgattaaa cgctagattg atgacgctga aaccgctaaa
    13201 aatggataga aacaacccat aagacaagaa attaaaggtt gtgcccgtca acagcttttt
    13261 catccgctga ctatatcccg cacttagctc aacttctcca gtaaccgaga gtgccatttg
    13321 tgtctgttcc atagctgcat cgattgtttt atcttcttct tgaaacagaa tcagtgtttg
    13381 caaaaaggtg ttcacttgct gtgtaacaac tgttttagaa aaggcatctg ggcgactctg
    13441 tatcttgatc gtcgccatct ttcctacttc gacttgttcg gtaaagtctg caggcagatg
    13501 taagataatg tttacacgat taaaataaag agcatcatcg atttcttttt gtgaggttcc
    13561 atccaatgta acgactgttt ccttgtcatt caaatattta cgaaggccat cgactacagg
    13621 cgactcttct tcactaatca aggcgatttt cgcatcttgc agagaggtat tttcaacatc
    13681 aatattttgc gcaaaaaaga atgtggcaac aacagtgata attacaccta tcagtaaatt
    13741 cgatttattt gctttaagca atgtccaaat cgttttataa agcgtcataa cgcacccctc
    13801 tttccatcca agcatttcct aacgcaaaaa ggaaaataaa tccgatcaac catagaagat
    13861 tggtataaaa actagccaaa tcagtgtaga aataaagttt atacaaactt tcgctgatca
    13921 agttgacgag atttaattga ccaacaatcg gaatatgaag atcaatccaa tacttcactt
    13981 gttccgaccc catcatacca gcaaaaaagc tcatcgcaat cgtcactgaa atcaagatgc
    14041 tatccttttg caccagattc atttttggta tcaaattacc aaccaatgta cctaacaaaa
    14101 tcgcacacaa cgaccccaga gcggtaacaa tcatcaacca ttgccaacgt tcaccaaagt
    14161 ctacttgata gataaaacga aagacagcta acataatata aatctctgtc aaaacaatcg
    14221 taaagcttgc tgccaaatta gcgattgaaa ctaacgattg ctttctagga atcaaggaca
    14281 agcgaatacc atttgacgat tgatttgctt gctgatcctg tacattgcgg accccccaca
    14341 tcgtcccgta gagaatcgcc attccaatca gagtgaagaa ataaaaactt ttgaaactca
    14401 tattacgacc tgcatgaact tcttgaaaag atacttcttg ttcaaaaaca gtcatgatgg
    14461 gcgcgagatc aacgttctcc acttctgcca ctttactcga aattatacca atacgctgta
    14521 aaaattgatc catgacgttt ttcatcaacg tctgactcat tcctgcttga cccacatgga
    14581 gcgaaacaga tgaaccatca atgagataaa aaccagccaa atcatctgca tttagctttt
    14641 cattcgcttc tacttccgat agttcctgta cttcaaataa tggtttatca ttgacttcga
    14701 cttgttctaa gactgtggta aaattttccg aaagcgctgt ttgatccgct ttgattacaa
    14761 ttccagtatt aatcgtttca agattgccac cttgatcaat ctcgccaaag gccgccataa
    14821 acatcaatcc taaaagaatt ggaaaggcca atgtccaaaa caacatggat ttatttcgca
    14881 gcagtatttt tgtgcgatat ttataaaaat gccaaaacat tatttcccct cctctagtct
    14941 ctcaaatcct tgccagtaat ctctaaaaag acatcattca aggacgcttc tttggttgct
    15001 atttttccgt aactgatctt ttcttcttgt aagattgtaa gaatcggcaa caatactgta
    15061 tttagttcag tagttgttac ttggagctga ccttttttat aagcgattcc atcagaagct
    15121 attgttcgct gaatcgctga caacacattt tctgggagta atgggatgtc gatcgtcaaa
    15181 cgatccgccg tccgtaccat atcttttaga gattctttgg ttcctttagc aatcacctgt
    15241 ccttgatcca tgatcacgat ctggtcgcaa agaatttcta cttcttccat gtaatgtgtt
    15301 gtatagacaa tggttgctcc ttgtcgattc agctccttga tactttctaa aattttattc
    15361 cgactttggg ggtctacagc tactgtcggc tcatctaaaa aaattaaccg cggcttgtgg
    15421 gcaatcccac atgcgatgtt caaccgacgt ttcaagccac cactaagttc tcctggacga
    15481 aacgaaacat atttttctaa tcccactaat tgaatcacct cttcaatcaa ggctttcctt
    15541 ttctcttttt cggaaacata taaaccgcaa aaataatcga tattttcttg cacacttagt
    15601 tcgtcaaaga ccgcaacgtc ttgaggaacg accccaatca ttgccttgac agcataattt
    15661 tcaggagtca ttgttgtccc gaacaattta atggttcctt tgtcataatt taacaaagaa
    15721 agcatacagt tgatggcagt cgatttccca ctgccgtttg gacctaacag acctaagact
    15781 tctccttctt gtacttgcag atcaaaatga ttcaatgcta caaaatctcc atagcgtttg
    15841 actaatttat caatttcgat aatcatcatg aaaaactcct tccaaatttc ttgttgatct
    15901 gactataata aagtgcaaga aaagaaacta gtgacgattg gctaaaccac acatgacatt
    15961 tgtcacaatg aaggagtctg ctatgcataa atctcggcta ctcaacgaaa ctagctttct
    16021 actggttatc tcattgcttt tgctggtaag tcaaggattc gttgctatgc aagttatcta
    16081 tttattgatc gccgttttat tcactggtgt cagttattta ctgcctacaa agccaaattg
    16141 gattttccaa gttattctat ttcttttatg tgccttttta cctgatcttg tcctgtttct
    16201 gccagcgacg atgcgagtga tcgcccctga agaaagccac cttgattact tgtttttgtt
    16261 aggttttttg ttgatttcga cggttggtaa tcatcccccc atcaaggtcg cctttgtcgt
    16321 aattttgctg tttttttcct tttacttacg aaaaaaagat caacgaacga ccgatcttga
    16381 gaaaaagtac attgtctcca aagatactca ttgggaaaaa caacaagagt tatcggaaca
    16441 aaataaagca ttggccctgt cacaagaaac acaattagcc ttgcaaattg tggaagaacg
    16501 gaaccgaatt gcccgagaca tccatgacaa tgtcggtcat ctgctgtcaa gtgcgatttt
    16561 acaaattggt gcgcttgaaa tgatcaatca agaaccaaaa ctaaaagaac cattagaaaa
    16621 tttaagtgcc accgttcaca ctgggatgga tcggattcgt caaagtgtcc atgacctcca
    16681 tgagacatcg atctcttttc aacactcctt agagtttctg atcgctgatt ttccttcttc
    16741 tgttacgatt gaaggaaact tgtttgaagg attgaatgaa acgcagcaaa actgcttctt
    16801 aatggtcatc aaagaagcct taaccaacag tatcaaacat agtcatgcca aaaccgttgt
    16861 tttacatttt cgaacgttgc cggcctttta tcgcttgcaa atcacgaatg atggcaccag
    16921 cccacaaaaa aattctgctc ccggtattgg tttgaaaacg atgcgtcaac gtgttcaaga
    16981 acaaaaggga caagtccata ttttccaaga aaaccagcaa tttcagttaa cgatcatttt
    17041 accaagggag ggacaccaat gataacagta gtcattattg atgatgatcc atttgtgacg
    17101 aaatcgttac aaaccatttt agaaagcacg aaagagatcc gtgtgttagg cattggtcat
    17161 tgtgccaagg atgcgctagt cttatacgaa acccatcgtc ccgatgtttt gctgacggac
    17221 atccgcatgc ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc
    17281 aaagccatcc ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt
    17341 tccatcggtg tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc
    17401 aaatccgctt ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa
    17461 atgatgaaga ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag
    17521 cttgcaatca tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg
    17581 tacttgagtg acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta
    17641 cgggatcgca cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac
    17701 acaaaaaaac ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc
    17761 aattgtcccg caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag
    17821 gattcgttgg attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt
    17881 ctagttttga ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt
    17941 ctttttccat ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta
    18001 atttatcttc ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat
    18061 atcacactga aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc
    18121 tttaaaatca agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa
    18181 tcatcggatc tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa
    18241 cagcaggttc tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt
    18301 ccactgtttc cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta
    18361 gcttttcttt tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc
    18421 cgaccaaaga atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat
    18481 tatccatgat ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag
    18541 tgacataggc ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg
    18601 tgtagagcaa attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca
    18661 tcttgaattc actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat
    18721 tccattcgtt tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca
    18781 ctttatcttc agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga
    18841 atacattcaa ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg
    18901 tgtcgttcca atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca
    18961 ttttatttaa cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat
    19021 cttgtgcacg gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa
    19081 tttgataagc atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg
    19141 gcagacgtcc ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc
    19201 gataaatctg attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta
    19261 cttgaccagg accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat
    19321 tcgtcggaat cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat
    19381 caacaatcaa aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc
    19441 cattcagccc cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc
    19501 gtttagctgc tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt
    19561 ttgaaggatc caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca
    19621 cagtagtttt ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt
    19681 cagcgatagc ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc
    19741 gaatattctt ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc
    19801 gctggatcga attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag
    19861 tctcttcttg ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat
    19921 caggctggat ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg
    19981 tctccgccgg tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga
    20041 tagctgctcg aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat
    20101 ctgcacgttt gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga
    20161 tgatatccaa agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga
    20221 acccgtaacg attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct
    20281 cagcaagcat ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat
    20341 cagcaatgat tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt
    20401 tcttcccgat tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg
    20461 ttggcttttc ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca
    20521 ctaattctcc ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga
    20581 ctacaatctc tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt
    20641 tgtaaaaatt actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca
    20701 tttcttctgg ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt
    20761 tcagttccta aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa
    20821 ggctcttcag tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac
    20881 tcacttagct cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct
    20941 acgaataaaa gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata
    21001 cgtgtgattg ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa
    21061 tgattacgca aattcggcag attatccgga tatttctggt acatttcatc gatcgactga
    21121 ccttctaaag tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga
    21181 cgagcaagct ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa
    21241 taaattgctt taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa
    21301 ctttgtggca aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat
    21361 tgtgtcttcc catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt
    21421 tattttactc tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat
    21481 tctaaattag ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc
    21541 gataaattca atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg
    21601 ttttcacgca taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc
    21661 tcttctccat cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa
    21721 tgtagtttac gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata
    21781 tttacatcat tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa
    21841 cctagtggct cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt
    21901 ccagtgtttg cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct
    21961 ctcatattta tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta
    22021 tcaactcggt gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt
    22081 atcagaacag gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc
    22141 tcaacttttt tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt
    22201 tccttttaat aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc
    22261 tttgattcgc tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt
    22321 ttcctctggt atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc
    22381 agcaaacttg gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata
    22441 aaaagcctga cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc
    22501 atcttctgag agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac
    22561 cgcgttgaat gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc
    22621 ttcggcagat tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc
    22681 attttgaatc tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac
    22741 atctaatagc gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata
    22801 catgccagat tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt
    22861 aaccgcatgg gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt
    22921 tttgcatatc aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa
    22981 cagctgatga ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc
    23041 ccctcatttc tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga
    23101 tacaatgcct agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc
    23161 gctgaaaact acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg
    23221 tgtgacaact tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta
    23281 agttatgctg gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac
    23341 ttcgtctata acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa
    23401 ttagggacgg atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag
    23461 gccttagaac tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt
    23521 gacagtattc ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt
    23581 gaccgtatct tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg
    23641 cttaaagaat tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc
    23701 actgcagaga atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc
    23761 aaaatcgtgg atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat
    23821 gcttattgca aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat
    23881 tgaaataaat cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta
    23941 ctgctggatc aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg
    24001 ccactgtgat tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt
    24061 tccaaaaacc tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg
    24121 tcaagacttc agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa
    24181 gtcgacgaac agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca
    24241 gcgaaatgaa gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta
    24301 aagaagcggt cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg
    24361 ctgcctttac tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat
    24421 cgctagcagc ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca
    24481 aatgatcatc ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg
    24541 ccacatcttc ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag
    24601 aaatcattgc gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct
    24661 gaccttcttg attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct
    24721 tcagcacgta catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg
    24781 tcatgatcgc gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat
    24841 cgtctgccga catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca
    24901 agtcaacagc attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca
    24961 tttcatctag gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg
    25021 ctaattcttt aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt
    25081 ggtagaattg cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa
    25141 tattattttc ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt
    25201 catccaaacc aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc
    25261 tgcggctaaa ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag
    25321 atcataacaa tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc
    25381 atcaccgagc agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc
    25441 tgtatgcagc tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc
    25501 agtctgtgcc gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg
    25561 ggcgatcggc aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa
    25621 ggcctgatat tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc
    25681 aaatccatgc tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac
    25741 atggattact tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat
    25801 tgtcccattt ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac
    25861 cagaacgtgt tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc
    25921 atgttctcct tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa
    25981 gacattttct acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa
    26041 cagacccttt gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt
    26101 ctcataatcc catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat
    26161 gccaaaaatg catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat
    26221 aaattgagcg gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt
    26281 ctgtattttc caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca
    26341 aagtacgaaa cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac
    26401 taggatggat cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag
    26461 aaatcgtcaa tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg
    26521 ctaccatcgt acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt
    26581 ccggtgtttc atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt
    26641 cccggttcgt tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt
    26701 aaaaccctaa atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt
    26761 agtgatggaa cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct
    26821 catgatcatc tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt
    26881 ctatagtctt tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt
    26941 tcgctttctt tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg
    27001 gagaataacc gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac
    27061 tgataggcag cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct
    27121 tgatatttat aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga
    27181 atctggattt ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc
    27241 accagatgat aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg
    27301 agaactttca tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca
    27361 aactgacaca tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt
    27421 ggaatatgcc gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg
    27481 acaaattcaa tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt
    27541 ttcactttca attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt
    27601 tccatagctg atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag
    27661 ttttttcgta ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa
    27721 aaaggagtcg atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag
    27781 caagagtacg agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag
    27841 actaatattt atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg
    27901 atccgtttcg ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt
    27961 ctcgaaacaa cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt
    28021 ctgcctcgtt caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca
    28081 ttaatccaaa ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt
    28141 ttagctattg atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta
    28201 ggggatgcat ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct
    28261 tatcgtcctg ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc
    28321 attttcacaa ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga
    28381 aagaatctag tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc
    28441 tatttatttg ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa
    28501 tttatcgaag aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct
    28561 ttggtcaatt tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat
    28621 cttgaggaac gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg
    28681 gcgcagtttc aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc
    28741 ggctgtaatg acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc
    28801 ggcgatttga atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt
    28861 tctgatcaaa aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac
    28921 aattatgcct gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt
    28981 tggagaatca cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa
    29041 catttacata caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct
    29101 aattaaaa
//
LOCUS       DACCKU010000029        28134 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.031, whole genome shotgun sequence.
ACCESSION   DACCKU010000029 DACCKU010000000
VERSION     DACCKU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 28134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.031"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(135..2288)
                     /locus_tag="IVN13_001203"
     CDS             complement(135..2288)
                     /locus_tag="IVN13_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP7011702.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2582..3430)
                     /locus_tag="IVN13_001204"
     CDS             complement(2582..3430)
                     /locus_tag="IVN13_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7011703.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="IVN13_001205"
     CDS             complement(3654..5594)
                     /gene="thrS"
                     /locus_tag="IVN13_001205"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP7011704.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5931..6110)
                     /locus_tag="IVN13_001206"
     CDS             complement(5931..6110)
                     /locus_tag="IVN13_001206"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP7011705.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="IVN13_001207"
     CDS             complement(6157..7980)
                     /gene="cadA"
                     /locus_tag="IVN13_001207"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7011706.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8258..10537
                     /locus_tag="IVN13_001208"
     CDS             8258..10537
                     /locus_tag="IVN13_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7011707.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10630..11277)
                     /locus_tag="IVN13_001209"
     CDS             complement(10630..11277)
                     /locus_tag="IVN13_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011708.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11456..12175)
                     /locus_tag="IVN13_001210"
     CDS             complement(11456..12175)
                     /locus_tag="IVN13_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7011709.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12306..13457)
                     /locus_tag="IVN13_001211"
     CDS             complement(12306..13457)
                     /locus_tag="IVN13_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP7011710.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13674..14414)
                     /locus_tag="IVN13_001212"
     CDS             complement(13674..14414)
                     /locus_tag="IVN13_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7011711.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="IVN13_001213"
     CDS             complement(14418..14741)
                     /gene="rsfS"
                     /locus_tag="IVN13_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP7011712.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14798..15391)
                     /locus_tag="IVN13_001214"
     CDS             complement(14798..15391)
                     /locus_tag="IVN13_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7011713.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15375..16022)
                     /locus_tag="IVN13_001215"
     CDS             complement(15375..16022)
                     /locus_tag="IVN13_001215"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP7011714.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="IVN13_001216"
     CDS             complement(16035..16346)
                     /gene="yhbY"
                     /locus_tag="IVN13_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP7011715.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="IVN13_001217"
     CDS             complement(16362..17471)
                     /gene="yqeH"
                     /locus_tag="IVN13_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP7011716.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17468..17995)
                     /locus_tag="IVN13_001218"
     CDS             complement(17468..17995)
                     /locus_tag="IVN13_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP7011717.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18175..18960)
                     /locus_tag="IVN13_001219"
     CDS             complement(18175..18960)
                     /locus_tag="IVN13_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP7011718.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18953..19822)
                     /locus_tag="IVN13_001220"
     CDS             complement(18953..19822)
                     /locus_tag="IVN13_001220"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP7011719.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19823..21202)
                     /locus_tag="IVN13_001221"
     CDS             complement(19823..21202)
                     /locus_tag="IVN13_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP7011720.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="IVN13_001222"
     CDS             complement(21210..21629)
                     /gene="fabZ"
                     /locus_tag="IVN13_001222"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7011721.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21654..22130)
                     /gene="accB"
                     /locus_tag="IVN13_001223"
     CDS             complement(21654..22130)
                     /gene="accB"
                     /locus_tag="IVN13_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP7011722.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVISEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="IVN13_001224"
     CDS             complement(22133..23368)
                     /gene="fabF"
                     /locus_tag="IVN13_001224"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP7011723.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="IVN13_001225"
     CDS             complement(23387..24124)
                     /gene="fabG"
                     /locus_tag="IVN13_001225"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP7011724.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="IVN13_001226"
     CDS             complement(24136..25053)
                     /gene="fabD"
                     /locus_tag="IVN13_001226"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP7011725.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEATPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25160..25384)
                     /locus_tag="IVN13_001227"
     CDS             complement(25160..25384)
                     /locus_tag="IVN13_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7011726.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25437..26399)
                     /locus_tag="IVN13_001228"
     CDS             complement(25437..26399)
                     /locus_tag="IVN13_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP7011727.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26409..26849)
                     /locus_tag="IVN13_001229"
     CDS             complement(26409..26849)
                     /locus_tag="IVN13_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7011728.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27155..28120
                     /locus_tag="IVN13_001230"
     CDS             27155..28120
                     /locus_tag="IVN13_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7011729.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         7781 a         6185 c         4877 g         9291 t
ORIGIN      
        1 ttttttgccg agtaaacggt gttcaaaagc cagtttctcg gtatgaagtc aaatttaagc
       61 aaaaatgtga gaagctattt tcacaaaatt tttcttcata ctcgaaacta agcggctttt
      121 tcacgacctc ttttttattt tgccatgaac atatcataat acgcgtccat tatttcttta
      181 gcaatcgtca agttcatgtg gtcttgttcg tctaataagt tcggcaagac aacactgatg
      241 gcaatttccg gattgtctgt tggtccgtat gccacgatat tactattgac tgtcgtaata
      301 tctggatgac cttccgcaac cgtttctgca gtccctgttt ttgcggaaag gtccattttc
      361 gcactggcta atggtttggc tgctgtgtaa ggatcggtac catggacaac ttggtggaat
      421 ccttcacgga ggatcgccat attttctgca gaaatattga ccgtattttc tactgttgtg
      481 ccaatttctt tcttcacttc acccaagttt ccattttcgt cattgccata gatcgctttg
      541 actaggtgcg gctgtactcg tttcccgcca ttagcgactg ttgaagcata ttgagctaat
      601 tggattggtg tatatgtgtc aaactgtcca aatgccaaat cggtaaagtt acctggagta
      661 taccattctt tgccattatc cgcattgaac ttcttcatat aatcaacaga acgtgagatc
      721 ccaggagatt cattcggcaa atcgatccca gtcgttgtac caagaccaaa ctctttgaaa
      781 gagtctctca attgctgata agctttagct tcttcatcta aactaggtag tcccattcca
      841 ggtgtatagt ctagtcctaa catctttaac gccactttga tcatatacgt attagaagac
      901 aattccaatg ctttgactgc atcaagagca acttgtccgc ttcgattaaa gacagagctt
      961 ttttctgaag caccttgcag acgaatcggc tcatcaatca aaacttgatt tccgctgatc
     1021 gcgttacttg cccagcctgc tgtcaaagta ccagccttga ctacagaccc tggagcaaag
     1081 gcgctagtaa tcgtgcctaa agcattctcg gtgatttctt tggaaccttg ttcatgactg
     1141 aaaccagtca ttgctagcac ttctcctgtc tgaggattca tagccactgc ataagcacca
     1201 ggcgaatact gtgctttgcc gttgttgatc aatgtttgat aattcctttt caaaatttct
     1261 tctacttttg attggaattc tgcattcata gataacatta aattagaacc tttttcccca
     1321 gagaagattt ctttttgatt cgaaacattt ccttcattat caagggaaac ttcatattgg
     1381 gttttggttc cttgcaggac atcttcatat tgtttttcca aataactttg tcctacacgg
     1441 tcattccgag aataaccttt tgctaggtat ttctctgctt catctgctgg aagtccttgc
     1501 ttttcagtcg tcacactgcc aagaatactt ttcagtgaac ttgctgcatt atattctcgt
     1561 gtccagtctg tgccagtcga aactcctgga agttcagaag cgcgttctgc cacaacagct
     1621 aactcttgat cagtaacacc agagtttttg atatagacag tgtttaattc atatgcacta
     1681 ttcatccgtt taaagatggt agcaattttt aattgctcct cgtttaaatt aatctcatca
     1741 gctgtgactt tattgaccat cgcactatat tgttcgctgt tccctaattg cttttctttt
     1801 gccgaaagtc tttttgtggc ttctttcaaa tgtttgggat cagccagcca aaaatctttt
     1861 ttatctctat ctgtcagatt ttcatctacc ggaacatcga tcagcgagtt cagcttcgtt
     1921 gcgatttcaa gcaaatcttt agccgtaact tgtgttcctc tcgtatatgt gatggcctga
     1981 ttcgctttat tcgtaactaa agcttttcct gtggagtcgt agatcattcc gcgaggagta
     2041 ctttcttgga ccgtaatcgt agaagtagac ttcactcgcg cctccatctc ttcgccgttc
     2101 acgatctgca aataacccaa tctagcaatc aacgcaacaa ataacgtaaa tataatgaaa
     2161 aaaagaaaat ttaagcgaaa aggaacatgg gaccggcgaa acgttttgtt cttaggttct
     2221 tgtgcacctt ttcttatttt ttctaaccaa gaagtattta atattttttt cataaaatct
     2281 ttcatcattt cctatccttc cgcctattta cattcatgct tcattgtacc caaaaacttc
     2341 ttaataaaaa agaaaaaatg ggcggagcaa gaaattcttt acctaatttc aagataaaat
     2401 caactttttg tttcacagca atcacttaaa gttacatatc caactatttt tttcattaaa
     2461 aaataaactt atttttgaaa attatgtttt cttaagtcgt gaaacacttt gatggatcat
     2521 ttatttgtgg aaaactacgt tttgttattc taaaaaactt atataaaaaa tagtaaggtt
     2581 tttatttcga atccataaaa tattcttgga ttgcttctcg tgtttttttc actttttcgc
     2641 taagcttttc gtttttagcc atatagaaat aaaaaatgat atcgatcgta gcaaattggg
     2701 ccaaaagtga attagttgcc gcacttcgat tcgtgatttc ttttggtcta gatgtttgta
     2761 cgagtacatc ggctatcttg ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt
     2821 cgatattcat gcttttcgcc agctcggcta atgcgactac atctgcactt ctacctgagt
     2881 ttgaaacaag ccaaagcact ttctttttct cattgctgac aagttgcggg agcaagacgt
     2941 ggatatcttt ttcaaaaatg attggcttac cgacacgaga ccatttttgc agaatatctt
     3001 ctactactaa agaagaggcg ccaaccccat ataaaaaaac agtctctgcc gattccaaac
     3061 actctacgac tttttggatc gtttcttctt ctagaatatc tactgtttct tttatcgcta
     3121 cttgtgcatt gttaccaagt ttgtttttga tagagtaaaa ggactcattt gcttctatat
     3181 cactaaaacc ttcatcgatt ccagtctgta gttctgttga aatcgtgatt ttgaagtccg
     3241 tcagactttg aaaaccgatt tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg
     3301 aagcttctgc taattcatta gctgtcattg tagggacctt ctcaatatta ccgataatgt
     3361 agttggccaa ctttttttct gctgaagaat aatcttccat catactttca atacgactaa
     3421 ttacgctcat aagttcctcc acccttttaa tgatagtcta tgtaagtata ccatatcaga
     3481 cccttattca cggtcgtata ctggataaaa aacaaaaaaa caatccgccc aacagaatat
     3541 cttatcccat caatcccaca gattctattg agcatctgtg tctttgagga atacctgtta
     3601 tctattgttc ggattgcttt ctatctacta cttttttaca ggaaatttac ctattagttt
     3661 tctctgctgt aatttttgac ttctgcttcc atagcatcaa taaagatatt caaatcttct
     3721 acagctgttt ctttacttcc ataacgacgt acgttcactg ttgcatcttc caattccttg
     3781 tctccaacaa ctaattggta agggatcttt tgtgtttgag aggcacgtat cttatagccc
     3841 attttttcat tacgatcatc aacttctaca cgcaacccct tcatttgcaa acgttctttg
     3901 atttcataag cataatcgcc atgtgcatca acagaaacag ggatgattgt tgcttgtata
     3961 ggtgccaacc atgttggaaa agcgcctttg tatacttctg ttaaataagc gacaaaacgt
     4021 tccattgtag aaacaattcc gcggtggata actactggac gatgcgtatt ttccccgtct
     4081 tctccaacat atgtcaaatc aaagcgctct ggcaataaga agtctaattg tatagttgat
     4141 aatgtttctt ctgtaccaag agctgttttg acttgaacat ctagttttgg tccgtaaaag
     4201 gctgcctcgc cttctgcttc aaagtaatct agttctaatt catccatagc ggctttcagc
     4261 attgcttggg ctttttccca cattgcgtca tcatcaaaat atttatctgt attgtttgga
     4321 tcgcgatagc tcaagcggaa acgataatct gtaatattga agtcagcata tactgccacc
     4381 atcaattcaa gcgtacgttt aaattcatct ttgatttgat caggacgaac aaatgtatga
     4441 ccatcattca acgtcatttc tcgtacacgt tgtaaacctg acaaagcacc tgatttttca
     4501 tagcgatgca tcattcctaa ttcagcgata cgaattggta attcacgata agaatggatc
     4561 gtgtttttgt agaccatcat gtgatgaggg cagttcattg ggcgaagaac aagcatttcg
     4621 ccatctccca tatccattgg cgggaacatg tcttcttgat aatgatccca gtgtccagat
     4681 gttttgtaca actcaacatc tcccattatt ggtgtatata catgttggta acctagacta
     4741 acttctttgt ctacgatata acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc
     4801 cagaatggca aaccagaacc tacttctggg gaaaccatga acaagtcaag ttcttttcct
     4861 agtttacgat ggtctctttc ttttgcttct tcacggagac ggatatattc tttcaaagct
     4921 ttcttgtcaa aaaaggctgt cccgtataca cgttgcatca tttgattgtt tgagtttcca
     4981 cgccaataag cacctgccac tgataataat ttaaatactt gtatacggcc agtggatgga
     5041 acatgtggtc cacgacacaa atcgatgaat tcgccttgtt gataagcagt aatcacttct
     5101 tcttctggca attcactaat cagttctact ttgtacggat cacttgcaaa aatttctaat
     5161 gcttcctgtt ttgaaagaac acggcgttcg atcggcagat tttctttgac gattttcatc
     5221 atttctgctt cgattgccgg caaatcttct gcagttactt ggttttgtcc attatctgta
     5281 tcgtagtaaa accctgaatc gatagctggt ccaacgccaa agtggatatt tggatagagg
     5341 cgacgcatgg cttgggccat caaatgagct gctgaatgac ggactaagcc aagtgcatct
     5401 tcatggtctg gtgtgatgat ctcaatactt gcatcttcct cgattggacg agtcaaatca
     5461 acgagttttc cgttcacttt accagctaaa gctttttttg ctaagctttt tgaaatgctt
     5521 tcagcaattg ccaatgttgt tgttccagac tcaaattctt tgactgcacc atctggaaaa
     5581 gtaattttta tcataatagt ttcctccttg aaatgttttg attgatcaga tcatcaagct
     5641 gaaaaacaaa aaaatcctag catctcttga aaagagatac taggacgaaa agttcgtggt
     5701 tccacctaat gttcaaacgc aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt
     5761 gcttcaacaa gggtttcgaa agtggtcatc ctttggtttt ctcctggaaa agtttcagcc
     5821 tagcttttcc tctcttatcg aagactctca aattcagttg gctttctcat caatcaatta
     5881 tgtgtttatt taaccactaa taaatagaaa aatcaagtgg gaaactaaac ttattttttc
     5941 cattccccat ttacaccgta tgttcctttt ttcatctcat tgatgatgac atggatgtgt
     6001 tcttttggtg cctgcgtgtt tttatgaaca gcttccgtga tatctttcat catattttct
     6061 aattgttccg gactgcgacc ttctactaat tcgacatgta cgaatggcat aatgctctct
     6121 cctctttctg atcgttcttt tttcaactcg attctatcat actatgtttg tcttgggtac
     6181 tgaatcagga aaaatggctg gatctttttt tgctctcaag gcactcaaaa tagagaccgt
     6241 atccacgact tcttgcaaaa cagctccgaa taatgcgggg attagtcccg tacttgcaat
     6301 taacatcaaa aaaatacaaa taaagatgcc aatcagtaca gactgtttcg ctattctcat
     6361 cgtatctctt gaaatcacta ctgcttttgc tacacgcaag agatcatctt tcaagatgac
     6421 cgcatctgca ctttcactag cagctgtaga accatgagcc cccattgcaa taccgatatc
     6481 tgcagcagca agtgctggtg cgtcattcac gccgtctcct accatcgcaa caggtctttg
     6541 atctttaggg atactttcta aaatggtcag tttatcttga ggcaagctac gggcatgtac
     6601 agaatctaat tggacttttt ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat
     6661 catcatcaaa tttttcacat caagttttct tagctccttg attgtctttg ctgcttctgg
     6721 gcgaatctga tccgaaaata ggatcgttcc tgcaaaagat cgatcaattg ttacatagat
     6781 tgctgtattg gacgtttcta tttcttcagc tgaaacaaaa gaggcacgtc caatctttac
     6841 ttcatgatta ttgactactg cttggattcc ttgaccggtc acttctttca gttgactaac
     6901 aggatacagt tggctgtttt tcacgttgtt cactaacgaa cgagctaaga tatggctaga
     6961 ttcctgttct gcactagctg catacagaag tagctcttct ttttttattc cattgactgg
     7021 ctgaatcgcc tctacttcca atatcccttt ggtaagcgtt cctgttttat caaaagcaat
     7081 cgtttttgta cgtgcgagct tttcaatcgt tgttccagtt tttacaacga tcccgttttt
     7141 acttgaacgg ctcattcctg caactaaagc aatgggcgca gctagaatca gtggacaagg
     7201 tgatgccact accagtacct cagcaaatcg taaagggtct tttgagaacc accaagaaat
     7261 accaccgatc aaataagcaa tcaacgtgaa agggactgca taacgatcag ctaaacggac
     7321 aaatcgggca ggcttttctt ttgattcttc gactagttta ataatcattt gatactggct
     7381 gtctgcggca cgtttttcag cgatcatttg gatcgatccg tctccattta aagaccctga
     7441 cataatctga tctccaggtt tcttggtttc cgggcgggac tcacctgtca aagaagattc
     7501 atcgaccgaa gattctcctt tggtcactcg accatctgcg ggtacaagtt cgccaggctt
     7561 gactagtaac tggtcatcaa tatttatttc atcgattact atatcctgca attttccttc
     7621 tttgaatcga tgggcaattc ttggggtgtt atctaacaaa gactgcagtt ctttacccgc
     7681 tcgtttattc gcgtaatcct ctagactatc tccgcccgta agcatgatca atatcatcaa
     7741 actagcccag tattctttga cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc
     7801 tacgccatat cttcctgaac gtaacgtctt gatcatttcc aaaaacatca agaaggcgag
     7861 aatccctcca gtaaatgcga caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc
     7921 acttaataaa gctatcccac caataagcaa agtaagtaaa aattttttga aatgtgtcat
     7981 tgttttttct cctcttttat gtgacttaga gaggttagta ataaatttcc agctgcctct
     8041 ttcatcttaa gcatagtcct tgactagcac tttttaaaac aataggcctt aattgaaaac
     8101 aactatcact atcagcgttt ccaaaattca tcagttctac aaacaaaaat attcagcaag
     8161 gattactttt tcgattcatc agttaaaaag tacctttttt cgaaaaacta tgctatactt
     8221 ttaattcgtt atttacggcg gaaggagcaa acatgcgatg aatgagaaac aactagaaca
     8281 aacgcacgtg gatgaaacga tcgaactaat ccagatcgaa caaaatatgc tggatacaaa
     8341 acaacaagaa ttgactcagg aaatgaatca ttcgacaaaa gacacagcta atcacaagat
     8401 tcgagctggt tccaatgaag ctttttatga atctgctgtt gaatatcgcc aacatgagca
     8461 ggaattgctt ttgaaatacc atactcttga agcacagcaa aaacgcctgc aaacgttgtc
     8521 tgtcatgaaa gatagtcctt attttgcccg tatcgacttt aaagaagacc gtgaaacaga
     8581 aacattatat ctaggtatcg cttctttaag agatctggca gaagaaacaa tcgttattga
     8641 ctggcgcgca ccaattgcca atctttatta tgaaggtgag attggtcccg cattttatga
     8701 aactgaaaca gatagaatag atgtcgatct gcagttgaaa cgacaattta aaatccaaga
     8761 aggacgtatt ttatctatgg tagatacgtc ggaagtaatc aatgatgact ttttgctaga
     8821 gatattggat gaatcatcaa gcaaccatat gaaaaacatc gtctctacga tacaaaaagc
     8881 acaaaatgca atcattcggg acacaacaag taaagtaatg ctgattgaag gaatcgccgg
     8941 aagtggaaag acatccgctt tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa
     9001 gtggctcgag gctgaaaacg ttcttctatt ttcacccaac catttgtttt ctgattacat
     9061 ctcgactgta ttgccttcat taggtgaaag cggcgtgcct acacagacat tcaaaaatta
     9121 tttggtgcag ctgctgccag agttttcttt actagatgaa cagcaacaag aaagtggttt
     9181 tttgctaggg gaaaaagatc cgatacaagt gatgaaaagc ggattgacct tagtcgatca
     9241 gattgatcgc tatatccaag gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat
     9301 aaatggccgt acgatcctat caaaggaatc gattcgccaa tggtataaag aaacaaatga
     9361 acaattacct ttacatcata gactttctct attacaaacg aagctcctga aaaaattagg
     9421 cggtctgcaa aaagacgaaa cacgtcagca atgggtcaaa gatctggcgg aagaacagct
     9481 ccaagaacta tacgcaacag atccaaatct tgaatataca gaacaaaagg agcgttcatt
     9541 acgtaaaaaa ctggccaacc aaatcgtacg taaaagattc cgtcgaatcc atcgagggat
     9601 catgaattat caatttgtca atataacgaa acaatatctc cactttctac aaactgtgcc
     9661 actttctctt ctaacacgac ataaaatcag cgagaccagg tggcaggaac atttgacaac
     9721 tgttcgtaca cacttgcgtc aaagagaatt gcgtcaagag gatgctgtac tattcttttt
     9781 actgatgcgc cgaacatatc cagtgacggt tacacacaaa gctcgctata tctttattga
     9841 tgaaatgcag gatttcgcac catcacaagt ggcattgctg caagccttgt tcccaaaagc
     9901 aaatttgact ttctgtggag atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg
     9961 atcattggaa gaccttttcg tggaccgtga agtcactcgt ttccaattaa cgacaagcta
    10021 tcgctccaca aaagaaatca ctgattttgc taatcatttt attgtgaaag aacatcaagt
    10081 agaaacaaca gcaagaaaag gagaccttcc tttgctcatc caaggaacaa cagatgatat
    10141 caaattgaat tggttagcag ataccattcg aacgactcag ggaaaagccc ggtattggcg
    10201 tacagcaatc atagctaaaa cacaagaaga atgcgaagct ctttatgctc aactgccaga
    10261 cgagttgaaa caacaagtcc agctgatttt aaatgagagt gactttatga agcgttccat
    10321 tattttactt ccagcctttt tagcaaaagg tctggagttt gaccgagttt tcttgtggga
    10381 tgtagatgca gaacattttt cttctcagca agacaaattg atcctttaca ccatgtgtac
    10441 acgtgcgatg catgaattga ccctttttac ctccaataat gaaagtcctt tgttaaacaa
    10501 tatggatatt tcatcttatg agaaaaaagt actgtaagta aaaagacaac tgctatcatt
    10561 aatgttagca gttgtctttt tatttacaat aataattgca gaaaaagtaa ttcaatcatt
    10621 taaggtcatt tattctatat ataaatccaa atctgtttca ggatatttta atttgacaag
    10681 cgaaataatc attttcagtt cttccatgtt ttctggatat tctgggtcaa ataccttgct
    10741 atgacggcca atcccgccat tgtcgatcaa tcggtaaatt cgattccatt caatgaattg
    10801 gtcaattgat tggaaattgc tgattggata gccgccacaa tccaaaatct ctgatacacg
    10861 aaatgctagg tatgcactat cgatcacttt attttttttc gtgtctatct tctcatttct
    10921 ttcgataaga attttttcag gacgatgttt caagctccac tctaatagta gaaagaaagc
    10981 ctccatccga tactcttctt caaataaccg aaaggctgaa tcagaacgac gaaaaagcag
    11041 cggaatcaga taattttgtc tataaatcag cattggtgcg cggtccacca tacactctaa
    11101 ataacggttc agataccctg taccgacata gtttcctaat cgatagcctt caacgccgaa
    11161 tcttgcgtat ttttgcgcta attttgaaca atcagttacg ttcacagcta actcttctac
    11221 tttgcggtaa ccgcttaaga tacgttcaaa aggttctaaa gtgatttttg cttccatatt
    11281 cttccccgtc catcttccac atacttcata tcatttcaca tgatacttat gctctcatca
    11341 taccacaggt tgaattagaa aagaaaataa atcacacaaa aaagcaagac aatctgttag
    11401 tgagattgtc ttgcttttcg aaaaagactg aatacttttc gttaaaactg gttgcttagt
    11461 cttctaatgc cacattatgg aacacatgct gtacatcttc tagatcttcc agtgcatcaa
    11521 tcattttttc aaatgtttct tgatcttctg gagatagtgt tacctcgttt tgagggatca
    11581 tttggatctc agcgattgaa aaatcagtga ttcctttttc ttttaatgct tcttgtatac
    11641 tatggaaatc cgctacttca ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc
    11701 gaacatcgat gtctttttcc attaggtatt caaggatctc gtcagcgtct tctcctgcaa
    11761 aagcaaagat tgctgtattg tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc
    11821 cgccattttt cccaaatgct gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca
    11881 atgtatcaac aatcaccatt gagccgcttg gtccaaatcc ttcatagcgc aattcagagt
    11941 atgtttcatc tcctgcacct tttgcttttt cgattgctcg gtcaataata tgttttggta
    12001 cattataagt tttagcgcga tcaatgacga aacgtagttt ttggtttgca tgcggatcag
    12061 gatcacctga ttttgctgcc gcgtagattt caatcccaaa ttttgcataa atccggctat
    12121 tattagcatc tttagcagtt ttctttgcta cgatatttgc ccatttacgt cccatatttt
    12181 cacttcgctt tctgatttga tagtttcata acgcaaaaac gtcctgttcc attatacttg
    12241 tgaatcacga ttctgtgaag ctttttttct ttcttccttc attcttctca tttttagtca
    12301 attatctata ctcggattgg gatttgcccg aaattctgtt ccggaatatt gcctcccaat
    12361 tgatagattt gatcacttct aactaacagt tccgcttctt gtgcctcttt ttttccgatt
    12421 cgggaataaa tagaaatctc ttttttttct tttaagaatc ttcttccagc atcggtaaat
    12481 ccaagtagtc gaagataatt tttattttgt tggacaggaa tctcttgctt tttgatattc
    12541 agcaagacat aacaagataa tcgttggatc cgtgtccatg tatatctttt tgtttttacc
    12601 tttgacacaa aatcctcaaa actatcagca gttgcagctg ctgttttcat gcgcgattct
    12661 attccttcgg tcatctgata gatatctgtc agttccagca aagtagaaga taacaaccga
    12721 taacgcaaat acggccaata agattcccaa gtctgcagcg aacaagttga aagatcttct
    12781 tctgtctgag gcggcacaag tccagctatt ctttttcctt cttctaacgc atggcgaatg
    12841 gctgttgcac tggctatcct ctgatctgtg atgatttttt catgatagcc tgcactttta
    12901 cgtcggatcg gatgtaattc catagggtgg acatacttgg cattttcctt tgcataactt
    12961 aatcccaaga tatgatttgg cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga
    13021 aaaacctcag tcattttttg cggataacta tacgtttgat cagacagttc ttgaaatgcc
    13081 tcttctatcc tatcttgatt ctcttggaca aagcgtccga aactgctgta atcaaacgaa
    13141 gtctcttctt ctgttccaaa acacaaactg tcacagtcca gtgtttgcaa gatttttact
    13201 gcacctttag caaaatagtc agctgactga accgcccatt cgacgggaag ctccactact
    13261 aaatccacac cattttgtaa agcttcattc gcgcgatgcc atttatcgat gatagccggt
    13321 tcgcctcttt gcaaaaaatt cccgctcatt acagcaatga cgacctctgc tcctgttatt
    13381 tttcgtgcca tttccacatg atagcgatgt ccattgtgaa aaggattgta ctctacgata
    13441 atcccgcagc ttttcatgcg catcctcctt catagttatc gataaaaaag aaatagagac
    13501 aagtaaaaaa aggcaaaaat cggaaaaaat acccgaacta tataaactat tctcgcaaac
    13561 acaatacttc tgcgacgaaa agctgcactg caagagcaat gcctatatcg ttcggatata
    13621 taatttatgt tgaagattta ttcttttctt gttaactcga gcaggcaaaa ccgctagtct
    13681 ttgtatgcta caaagaacca gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca
    13741 gaaaatgctt cgactttcga aaagccgctg ttttccagca ttcttaaata actttccaat
    13801 ggataggtac gctcttgatg tagttcatct tcacgaataa atcgaccaga cccttcttgt
    13861 tcttcaacga aaaaggtaag aaaatgttcg atgctgtgtt tctcgcttcc tggataacta
    13921 tcccacaaaa aagcaaaatt atccccttga taatgatagc tatattctgg aaaaacctca
    13981 tctacttgat aggtggagtg tacgtcaaat aaaaatcggc cattttcttc taacgcttga
    14041 tagacttcat caaacacttg ctgtacctct tgtctatttt tcatatagca cagcgaatca
    14101 gaaaaacagg taattgcctg atattgtccg atttctgata aatcgagcat attgccttcc
    14161 acaaattgga tcgatgcgtc ttcttcatat gctcgctggc tggcaatcat caacatctct
    14221 tctgaaaggt ctaaccctgt cactttatat ccatcttttg caaaggcaac cgctaatgct
    14281 ccagtaccgc atgccagttc caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa
    14341 aaagctaacc attgctgata aagtgtttcg tccatcactt catcgtaaat gaaagcaaat
    14401 gtttcataag ccatatgtta atccaaccat tcactaagat cgactagcgg agcatctgac
    14461 catagttttt ccagattata gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca
    14521 tctccaagat cgatcaatac ccattttccg ccttcttttc cttcaatacg ttttacttca
    14581 tatttgtttt cttcttcttt atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg
    14641 ctatttgctg aacagatcat gaaataatct gccaacaagg aaacatttcg aacgtctaaa
    14701 gcgataatgt cttctgcccg ttttgaatct gctgcttcaa tcgcaatttt taattgttct
    14761 ttactatcta tgatgatttc ctcctaatat gagcagctta tttttttgct acccagtaat
    14821 tatatgtttc gattgttttt ggataaatct gttgttcttg cgcaagcaaa tgggccaatg
    14881 tatgttttgt ttcaaatgct acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat
    14941 cttgtacccc aggaaaatct cgacctggtt caatatagtc tgctacataa atgattttgt
    15001 caaggagact catcttagca gccccagtcg tatgcacccg gattgcatga agaatctctg
    15061 catcggtgat tcctaattcc cgctctacaa acgaagcgcc aacaagacca tgccagattg
    15121 cattattata attcaataac tccggatcaa aaccgtctct tacgattacc attttgaact
    15181 cctcatctgg tcgttctttt gcataatcat gtgtcaatgc agcaatacta gctttttcag
    15241 gagaagcacc gtattttttg gcaagagcca cggctgtttc ttctactcca agcacatgct
    15301 tgaagcgttg ttcgctcatt ctcatctgta ttttttgcat caggttttct ctagaataag
    15361 cagtatattc attactatag tccatattca tacaaccctt cattttgaat ataatcaatt
    15421 actttatctg gaacaaggta gcggatagaa cagccttctt tgatcttttg acgtattttc
    15481 gtggaactga tgtcgatctc aggtacatct acccaaatta caggatatgg agtatccgtt
    15541 gtgtatcctg gtcgacgtat acccacaaag ttgaccatag aagtcagctc atcgatttta
    15601 taccattttg gcaaatattc taccatatcc ccaccgataa taaagtaata atctgtatcc
    15661 ggattgtttt gcgtcaattc cttcatagta tcataggtgt aacttttccc accacgagct
    15721 aattcaatcg tttcaatctg caaaaaagga ttgtcttcca ccgctaattc caacatgttg
    15781 agtcgatgtt ttgcatcaat cgtctgtttc tcatcaacat gaggtggctg ataagaaggc
    15841 atcaaaaata cgcggtcaag acctaaattc cggccagctt gttcagccat cactaaatgt
    15901 gcaaggtgaa cgggattgaa tgttccgccc aagatcccta cttgcttttt cttctctaag
    15961 aattgaggca tttcttcagg aaataattgt gaacgaacaa atgtcttagc ttgcgtattc
    16021 atcatggtca ctccttatat tgcttttact tcttttgata gcctttgata cttttctttt
    16081 gtggaaggtt tgtataatac aataacacgt ccaatgattt ggacgatttc gcaatctatt
    16141 tctttttcta atacttgagc tgcttcttct gcaatctcat ctgtattttg aagtaacgat
    16201 actttgatca gttctctttt ttctaaagct tcttcaattt gaacgatcat tgctgaattg
    16261 atcccacctt ttccgatttg aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa
    16321 cgcttttgtt ttcctcttaa actcataatt ttccttcttc cttaaatcaa tgctttgcgt
    16381 ttcaatacat ctacaccctt tggcgcccag cccgcaatta ctactggcgc tgttacagtg
    16441 atccagccta gaccggcaaa aacgatgtct gttttttctt tcacagaaaa ctcaaaacga
    16501 acaagctccg gaaatgcctc gacttcatca ggacgaggag gctgtaataa tccacctaca
    16561 tgtttttgat aaaattcgtc tgctttttcc agtttcgttc gatgaatatt tacatcattt
    16621 gacacataag caataaatga agcacgattg ccgctgataa aatcaaatct tgcaagtccg
    16681 cccaagaaaa gtgtttgccc ttcattcagc tgataaactt taggtttgat ttcttttaac
    16741 ggtgcaatga tccgcaaatc ttttttacct aagtagtgcg ccatttgatg gcgatggata
    16801 attcccggtg tatcaatcaa aaaatgcccg tcatctaaag gaatctcgat tttatccaag
    16861 gtcgttcctg gaaactgcga agttgtgatt acatcttgta ctcccgctgt atttttgatg
    16921 atttgattga taagcgtcga ttttccgaca tttgttactc cgacgatata gacatcccgt
    16981 ccatcgcggt attcttcgat tttttctaat agttccgtca tctcatgaga tttcttagca
    17041 ctagttaata atacatctac tggacgaatt cctgcttcgt aagcacgttc cttcatccat
    17101 tgtgttaatt tagttttttt caatgattta ggtaatatat ccactttgtt cccaactaaa
    17161 agtactggat tgtcaccgac aaaacggtgt agtcctggga ttagtgaacc attgaagtca
    17221 aaaatatcca ccacattgac gatcaatgca tctttttgcc ctaattcatt cagtaagcgc
    17281 aaaaagtcgt catccgtcaa tgagacatct tggatctcat tataatggcg taatctgaaa
    17341 caacgctgac aatagacttc cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg
    17401 tatcctaatt caccgggttt gtttgtttgt ataacggcac cacagccgat acattgtaat
    17461 tcttctgtca ttcgattcca cctcgccagc ccatatcagg gtgttttgct aatagatagg
    17521 ccataattct tttttcccaa aaacgattaa attgtgtctt ccaactatct gttgcgataa
    17581 tgggttttac aaggatgcta cgtatccctg cacgattggc tccacgtata tcagtcataa
    17641 tctgatcgcc taccattact atctcatcat ctgctaaatc caattgtttc tttgctatgt
    17701 tgatccctct tgctaaaggt ttcattgctc gtgcaacata caaaagatca aatttttcaa
    17761 tcgctcgcgc gacacgtttt gaattattgt ttgaaacaac aacaacaggg attcctgcat
    17821 ttttcatttc taaaatccaa gtaagcaact cttctgttcc atctggatta ttccatgcaa
    17881 tcaacgtatt gtctaaatcg gtcaatactg ctttgatgcc tagattcttt aattgggcag
    17941 gtgtaatctg atagatggct tctaccatcc atgttggttt gtaatttgaa aacatgctgt
    18001 ctcctcatct gcaaaaaatg aaggagcaca taaagtactc ctcaaccccg tattatactg
    18061 catatcagca aaaaaaacta cctctatact cgttcgtctt tcagaaaaag catttcttta
    18121 caaaatgacc aaaaataaag attgatcaca gaaaaattta gaaacaaaaa acaactaata
    18181 ttggcggaac ctcttgtatc gtttttccag taatttatgc tgatctaatc gaacaagttc
    18241 cgtcaatctt tcaatcaacg cttttttcat aatgtgtgcg actttatatc gtggcaactg
    18301 ctcgccgttg aacgtttcag gaataactcg atcaacgatt cctaactcgc gtagttctgg
    18361 cgcagtaatt ttcatcaact cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat
    18421 cgacgcaaat ccttctggag aaagcacagc ataaatcgtt tcttccagca tccagacttc
    18481 atccgctaaa cctagagcaa gggcaccgcc acttccacct tcaccaatga tgatggcgat
    18541 gatcggtacc tttaaatcag acatttcgag cagatttctg gcaatagcct ctccttcgcc
    18601 gcgctcttct gctcctacac cgcaatatgc acctgctgta ttgatcaagg taatcaccgg
    18661 tctattgaac ttttccgctt gtttcatcag tcgtaaagct tttcgatagc cttcaggatg
    18721 gggagaacca aaatttctgc ggatattttc ctgcagatcc cgtccttttt gaataccgat
    18781 cactgttacc ggtttgccat ctaatcgagc gattccacca acaatcgctt cgtcatctgc
    18841 aaataaacga tcaccatggc attcctcgaa agaatcgaag atctcttcaa aaaaatccaa
    18901 gcttgtccat cgttctttag ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac
    18961 gatctatcca tcctttctgt gtatgcaatt caagaagcgt atggattttc tgtttcaatt
    19021 ccatgcgggg aacaatctga tcgacaaaac catgagataa taagaattca gccttttgga
    19081 aatcttctgg aagttcctgc ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga
    19141 tcaagctttg gggttctgct agaataatat cgccttccat tgcaaaactg gcagttactc
    19201 ctccggtcgt cgggtccgtt aaaacagtta gataaaaaag accttcattg ctatgccgtt
    19261 tgacagcagc agagattttt gccatctgca ttagagaaaa gattccttct tgcatccgtg
    19321 cgccacctga agctgtgaac aagacgactg gaagactttc ctttgttgct cgttcgaata
    19381 atctggtgat cttttctccg acaatcgttc ccatacttcc catgataaaa ttaggatcca
    19441 tcactccaat aatgaaggga atcccctgaa tggttgcttt accagtgagg actgcttcat
    19501 gaagtcctgt tttatcttgc atttttttaa ttttttccgt atattcggga aatgctagcg
    19561 gatctttggt tacaagatcc gtatcccatt cttcaaaact tttttcatca ataatcaaag
    19621 ctagacgctg ccatgcaccg attcgaaaag cgtatccgca atgagggcat actttttctt
    19681 caccgatgtc tttcgtataa aggatatgtt tgcaatttgg acactttgcc cacatgttat
    19741 caggaacaga tggcgagtta gcaggtttgc tctctctatt agggttgatc cgaatatatt
    19801 tctttttctt aaacagagcc atctatgcgc ctcctatctc cgtttctggt gtccaattcg
    19861 gtaaaaattc ttcttgtaaa aatgctgtgc tgtaatcacc tgctatgact gcaggatgac
    19921 taatcaaatc catctgaaat tctgcgttcg tgatgattcc ttctgtgata aattcactta
    19981 aggctcgctg catcttcatt aatgcttcaa aacgcgtatt tccatggacg attatttttg
    20041 caatcattga atcataatat ggcgggatcg tatatcccgg gtaaactgca ctgtctactc
    20101 gcagtcccat cccacctgct ggaagcagta agttttggat cttccctgga gaaggagcaa
    20161 aatggaaagc aggattttct gcattaatcc ggcattcgat tgcgtgtcct tggaatacta
    20221 cttcttcttg acacacattc aaaggctcac cggctgctat ctccacctgt ttttttacta
    20281 aatcgattcc tgtgaccatt tctgttactg gatgttccac ttggattcga gtattcattt
    20341 ccatgaaata aaaatcgcct tcttcatcca tcagaaactc gatcgttccg gcattttcat
    20401 aattgactgc tttagctgct cgaacagcag cgtctcctag tgcttctctt tttgtttgtg
    20461 agatcactac agaaggagat tcttccagta ctttttgatt gttgcgttgt aaagaacaat
    20521 ctcgctcgcc taaatgaatg acatgaccaa atcgatcacc taagatctga acttctatat
    20581 ggcgagcagg ataaatgatt ttttccaaat acatatcatc atttccaaat gccgctgctg
    20641 cttcttgctg agctgaagaa aaatgctgtg ggagctcttc ttttgattga actttccgaa
    20701 tgcctttccc gccaccacct gcagctgctt tcagcatcac tggataacca attctgtctg
    20761 ctatttccaa tgcttcttct actgtagata atacaccagt acttcctgga atgactggga
    20821 catttgcttc ttgcatcaat tgacgcgcat tgatcttatt tcccattgca tcgatcgttg
    20881 cacttttagg accaataaat gtgatattgc actcttcaca catggaggca aatcggctat
    20941 tttcagataa aaaaccaaat cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc
    21001 tcagtacttc ctgaacattc agatatgaat caacggcttt tgccgggcca atgcagattg
    21061 cttcatctgc taactgtgta tgaagtgctt cggcatctgc ttctgaatat atcgctacgg
    21121 tttggatccc tagttcgcgg catgcacgaa tgatacgtac agcgatttca ccgcggttag
    21181 ctatcaatac tttagaaaac atatttcacc tatccaatca taaaggttaa ttccgcttcg
    21241 gctacttttt taccgtctac tttagcgatt cctttgccta ttccggctga agctttcact
    21301 ttcaatatct caacttcaag gtataaggta tctcccggtg taactttctt acggaatttt
    21361 gctttgtcca atccgccgaa ataagccgtc tttcccttga attgttcaag agaaagtaat
    21421 gcaactgctc ctgcttgagc cattgcttca acaatcaata cccctggcat aacaggttct
    21481 tctggaaaat gtccttgaaa aaagggctca ttgatcgtta catttttctt agcaacgatc
    21541 cgttctcctt caaccatttc ttccactcga tcaatcaaaa gcattggata acgatgtgga
    21601 atgatttctt ttatttcttg tatgttcatt gttttccctc tttcctgctt tgcttatttt
    21661 tgctgaatcc taaagagtgg ctgtccatat tcgacaactg actcgttttc tacaaggatt
    21721 tctgtgatca ctccgtcggc ctcactagtt atctcattca tcagtttcat cgcttcaata
    21781 atgcagataa cttctccttt ggcaacttga tcacctactt ttttgaaagc tggtttgtcg
    21841 ggcgctggtt gcagataaac gattcccacg attggcgaaa caacctcgat tccatcaaat
    21901 actgccgatt ctttttcttt cattatttct ggagcagttg ttgcttgttc agatatgaca
    21961 ttgactggct ccgaaactgg caacgtttcg tttgtttgaa ccatattcat gcctcgactt
    22021 gttttatttt tattcatata caattcaaat tgaccttcac gcaaatcgaa ttccgttagc
    22081 gaggattgat caaattgact gactaattct ctgatttcat tgatatccaa aattattcct
    22141 cccagcgttt catgcacaga accgcattat gtccgccaaa ccctaatgaa ttactcattg
    22201 cataagtaaa acttgcaggt ttgctttcgt ttttgacaac tgtcaagtca atctcaggat
    22261 cttgtttttc tacattgatc gtaggtggga taaattgatg ttctaatgcc agcaaagttg
    22321 ccaaggcttc gatcccgccg gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc
    22381 tgacgtaagt atctctgaaa ttttcaccta atgcgatttg gattgctttt gcttctgcct
    22441 ggtcattggc tggtgtactt gttccgtgag cattgatata tccgacttgt tccggctgta
    22501 caccagcttc ttccatcgca agcttcattg ctttagatgc accagaacca tctggagtag
    22561 gagctgtcat atggtaagcg tcacagtttg caccgtatcc aacgatttct ccatagattt
    22621 tggcaccgcg agcttgtgca tgttctaaag attcaagaac tagaacgcct gctccttcac
    22681 ccatcacaaa accattccga ttttcgtcaa acggaatcga accacgatta ggatcagttg
    22741 attctgagag tgctgttaaa gctgcaaatc cagcaatccc catctcacaa atcgttgctt
    22801 ctgttcctcc agcaagaatg acatcagaat agccatgttt gatgttacgg aatgcttccc
    22861 caattgaatt cgttccgcta gcacaagctg ttacgatagc tgtacaaata ccttttgcac
    22921 ctacacgcaa agcaatattt ccagctgcca tgttggcaat cgccattggt acgaacattg
    22981 gagaaactcg ttttgcacct ttttcatgta gacgaataac ttggttttcg attgttggaa
    23041 gaccaccgat cccagatccg atgatgacgc caaaccggtc ttgattcatt ttttctttat
    23101 ctaaaccgct catctccatt gcttctaaag cagcgtagat tccatagatc gaaaacatat
    23161 ccattcgttt gctatctttt ttaataaaat atttatcgta agggaaatct ttgacttcac
    23221 ccgctaaggt aatccctgtt tctgaagcat caaatttagt gatcggtgcg atgccattcg
    23281 tcccctcttt taagctattc aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc
    23341 catagccagt aattaccact cgattcattt gtttgctctc cttttcttat ccgtgcatga
    23401 ccaatccgcc atcgacattg atgacttgtc cggtgatata tggattttgt gctaagaaaa
    23461 ctgctgtttg tgcaatatct tctacttgtc cgaaacgctg catcggaatt tgcttttccg
    23521 cttgttcctt gactttgtct gctaaaactt ccgtcatatc agttgtgatg aatcctggcg
    23581 caatggcatt acatgtaatg cctctagttg cagcttctcg tgccacagat tttgttaatc
    23641 ctactacgcc agctttacta gctgcataat ttgcttgacc aatatttcca atcaacccag
    23701 atacactaga gagattaatg atggcccctt ctcgctgctt catcattttt ttcaatacgt
    23761 gctgcgtcat attgaaggtt ccaatcaaat tgatatccag acattttttg aaatcttctg
    23821 catccattcg catcacaagt ttgtcatttg tgattccggc attgtttact aacacatgga
    23881 tcgatccaag cttttcttct gcttctttga tcatttgtcc cgctttctca taatcagaga
    23941 tgtcaccgga aactcctacg cattttacac cgaatgcttc gatttcttct atcttttctt
    24001 ttggaatctc tccgcgacca ttcaaaatga tatttgctcc agcttttgca aaagctaagg
    24061 ccatcgcttt gccgattcct cttgtactac ctgtgataaa tacatttttt ccttttacat
    24121 ccataacgtt cctccttata gtattgctat tgtttcatca aatgtctgct tattctcaac
    24181 acgcagaact ggtattgttt tatcgatttt tttcataaat ccgcttaata cttttccggg
    24241 gccgacttcg atgacttgtt cgatgccgat tgtttttaac ttatggatac tctcatagaa
    24301 acgaacaggt tgcatgactt gctgttcaag tagtcccgcg attgtttctt tcttcattat
    24361 ttccgttgtt gtgttgctta tgataggaaa agaaggttca gagaattgaa tctgacttag
    24421 gtccttcgcc agtttttttg ctgccggttc taaaatagct gtatgaaaag gcccgctgac
    24481 atttagaggg atcattcttt tgaacccttt ttcttttaat agtaaaacag cttcgtctac
    24541 tgctttttct tcgccaccaa tcacgatctg ctgaggtgta ttataatttg caggagatac
    24601 gatgccgtac ttgctagctt catgacagct ttcctcgatc gtttcaatcg gcgcattcat
    24661 cactgcaacc attttcccgc ttccagctgg ggtagcttct gtcatataag cgcctctttt
    24721 tgctactaac gcaactgctt cagtgaagga tagcgcatta ctagctacta atgccgaata
    24781 ttctcctaaa ctcagtccta aagctgcatc tgggatgatt ccatgctcct tcaataagcg
    24841 ataatacgcg atgctgactg ttagaatagc tggctgtgta tattgcgtct gatccaaacg
    24901 ttcattttct gtaaagcaaa gttcagccat atcgtagccg agaatctcac ttgcttcatc
    24961 aaatgtctgc tttacaacgg cttcttcata taaatctttt cccatacctt gatattgtgc
    25021 accttggcca ctaaataaga atgctgtttt catgtttttc tccaatatat ccagaatata
    25081 tgtttgattc ttcctattca aaaaggccga gacattccta tttcaggaaa tgtcccagcc
    25141 cccaacctaa ttttaatgat tatgcagttt gtttttctac gtaagtcact aaatcttgaa
    25201 ccgttttgat tccgtcttct gtttcgattt tcacgtcaaa ttcatcttca atttcattga
    25261 tgatttggaa taaatccaag ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt
    25321 tgatttcttc agtttctttt cctaattctt ctgcgatgat ttcttgaatt ttttcgaata
    25381 ccataataat atattcctcc gatattgttt gctttttatt tttataattt aacagtctac
    25441 aactccatga gaatacttcc ccatgttaaa cctccgccat agcctgtaaa tattactttc
    25501 tgctgttttc ctaaggtgat ctttccacta gaaactgctt catccaataa gatagggatg
    25561 cttgcagcag atgtattccc atatttatcc atattggtca ggaacttttc ttgcggcatc
    25621 tttacttttc gggcaatttt ctcaataatt ctgaggtttg cctgatgcaa aagcagataa
    25681 tcaactggtt tgtctttcat gacttcttgg atattgtttg caacgtctcg tacggcaaaa
    25741 tcaaaaatat ctctgccagt catttgcaaa taatacgaac cttctgaatc ttctgaatag
    25801 aacggatttt tattttgctg gtagccagcg gtcaatgagc tacttctggt accgtcagct
    25861 gctaattttt ctttcagcaa ttttttctgt ggattcgctt ctaagagaat cccacctgct
    25921 ccatcgccaa acaacacagc agtcgtacga tcagaccaat cgattagctt agatagtgtt
    25981 tcaccaccaa tcaccagtcc cttagttgag ccgcagcgga tcattttttc agccatgctt
    26041 aacgcataaa caaatcctga gcacgcagca ctgatatcaa aagcgatagc atgagttgct
    26101 cccaatttcc cttgtacttg tgctgctaca gatggtgtcg tgtaatcggg cgacattgtt
    26161 gctactaaaa taaaatccaa atctttaggg tttagatttt tctgttttat caatttttcc
    26221 gccactaaaa tgcataaatc agaagtctcc tgatttgtta cacatcttcg ttcacgaata
    26281 cccgtccttg acgaaatcca ttcatcagag gtatccatca tcttggaaag atcattatta
    26341 gtaataatct tttcaggaag gacgctggct gttgcagtaa tcattccaaa ttgttccatt
    26401 aaatatcctc acttatagtc ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag
    26461 gcatgctgtt cctccgtagc cattccgtct aagatgttct tgaccatttc acgatgaaaa
    26521 tgctgatgga cccgaaatag taactttcct tttttcgtta gtcccaactt tacaacgcgg
    26581 cggtcatctt cactgcggat acgttcgaca tagccttttt ttactaactt gttaatagca
    26641 gtggtcaagg tcccaacagt aattgatagt tctttggcta cttctgaagt tgtttttttc
    26701 ttgtacatcc cgattgcctc gatcgtgtgc atctctgtga ttgacagatc tttgaattga
    26761 gattttttca gctcagactc ttcgattgtc aaaatatcat tgaagacact aacaagataa
    26821 tcatttactg tttttaaatc tggttccatg ttccaccgtc cactaattag tttgattatc
    26881 aaatcttttg atgttcaaac tatatcttaa cttttttcaa aatgcaacct taaaagatca
    26941 tttttttact atatgagctt tttttaagat tttactcatt taaaacaact gataaatcag
    27001 catttataat ttcttttatt agaaaaatca tctatctcac atattttgat tatcaaagta
    27061 ttttctgtga acagaactat agaattccct aaaacttgtc cattatttca aaatttgtta
    27121 tagtcttttt attcaaagcg taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct
    27181 ccccatgctg ccgggtgtct atcttatgaa agaccgagat ggtaaggtca tctatgttgg
    27241 taaagcaaaa cggctcaaaa atagagtaag cagctatttt catcagaata aacagcattc
    27301 aaaaaaagta ttacgtatga ttcatcatat aacagatttt gatttcgtcg tagtagacac
    27361 ggaattagat gctttattgc tcgagtgtca attgatccaa cattaccgtc ctttttataa
    27421 ccgacaaatg aattatttca gtaattataa ttatgtacat attaccaata agggatttgt
    27481 attaacagat actcctacgg ctcgaacata cgggcctttt cgtctctata aaaaaatgcc
    27541 gtctattttg cgtataatgg aagaaactta tcaaatgcct tggttgtctg aaattagttt
    27601 gttagctttg cgagtccagc ttcctgacct acaggaaatg acttttgaac agaaaaaaaa
    27661 ggaactccaa gggctgtttc aaggccgaaa taaaaagcta ctgacttact tgaaaaaaag
    27721 acaacaacat ttcatctatc agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat
    27781 cgaattagtt acttatttta ttagacggat ccaggaacaa aagcagtttt taagaacacc
    27841 atcgctcacc ttcagcatgc cattagcagc agatgagagt cagaaaaagc actacctcgt
    27901 ttgttatggt caacttgcag aaacaatgat tacctctggt gacgtttctc ctgacttcta
    27961 ttatgaaaag aaagaagctc atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga
    28021 tcctgtacaa atcttgatca gttaccgcaa aaaattagaa aaagaacagt ttgaaattga
    28081 actgttaaac aaaaaagaag ctgaaaagca actaaattag ttgcttctcg gctt
//
LOCUS       DACCKU010000030        27886 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.032, whole genome shotgun sequence.
ACCESSION   DACCKU010000030 DACCKU010000000
VERSION     DACCKU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.032"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1153..1551)
                     /locus_tag="IVN13_001231"
     CDS             complement(1153..1551)
                     /locus_tag="IVN13_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011730.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1767..2822
                     /locus_tag="IVN13_001232"
     CDS             1767..2822
                     /locus_tag="IVN13_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAP7011731.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2842..3195
                     /locus_tag="IVN13_001233"
     CDS             2842..3195
                     /locus_tag="IVN13_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011732.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3377..3673)
                     /locus_tag="IVN13_001234"
     CDS             complement(3377..3673)
                     /locus_tag="IVN13_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011733.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3677..4495)
                     /locus_tag="IVN13_001235"
     CDS             complement(3677..4495)
                     /locus_tag="IVN13_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7011734.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAVNENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWMVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4721..4942
                     /locus_tag="IVN13_001236"
     CDS             4721..4942
                     /locus_tag="IVN13_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAP7011735.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKELMKEHEENDQSISHEDMLKELGL"
     gene            4944..5210
                     /locus_tag="IVN13_001237"
     CDS             4944..5210
                     /locus_tag="IVN13_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7011736.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5323..5862
                     /locus_tag="IVN13_001238"
     CDS             5323..5862
                     /locus_tag="IVN13_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011737.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGAYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKQQLDRIKEENNKPNLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            6356..6640
                     /locus_tag="IVN13_001239"
     CDS             6356..6640
                     /locus_tag="IVN13_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011738.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGVVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKNSFIGKLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            6914..7168
                     /locus_tag="IVN13_001240"
     CDS             6914..7168
                     /locus_tag="IVN13_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011739.1"
                     /translation="MNYTDEEQITLEYVFTNEKGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            7250..7435
                     /locus_tag="IVN13_001241"
     CDS             7250..7435
                     /locus_tag="IVN13_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAP7011740.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            7502..7897
                     /locus_tag="IVN13_001242"
     CDS             7502..7897
                     /locus_tag="IVN13_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7011741.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELFEK"
     gene            7887..8264
                     /locus_tag="IVN13_001243"
     CDS             7887..8264
                     /locus_tag="IVN13_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011742.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            8265..8661
                     /locus_tag="IVN13_001244"
                     /pseudo
     CDS             8265..8661
                     /locus_tag="IVN13_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8967..9365)
                     /locus_tag="IVN13_001245"
     CDS             complement(8967..9365)
                     /locus_tag="IVN13_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAP7011743.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(9556..10323)
                     /locus_tag="IVN13_001246"
     CDS             complement(9556..10323)
                     /locus_tag="IVN13_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7011744.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPNKLKKT
                     GEIVLPVKAIIKYMDVDQNLETELKNIVNTLQRQAVYEISNLDKEYKTYATVSAIDQI
                     HWDLEEQIITFNFSQEVIPLILSIKKKYPYFVFQDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LKIDFAKVRKGRNYNFAVQFNISSEFDEKPYREKKLG"
     gene            complement(10358..11581)
                     /locus_tag="IVN13_001247"
     CDS             complement(10358..11581)
                     /locus_tag="IVN13_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7011745.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            12562..13365
                     /locus_tag="IVN13_001248"
     CDS             12562..13365
                     /locus_tag="IVN13_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7011746.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            13845..14300
                     /locus_tag="IVN13_001249"
     CDS             13845..14300
                     /locus_tag="IVN13_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011747.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            14419..15477
                     /locus_tag="IVN13_001250"
     CDS             14419..15477
                     /locus_tag="IVN13_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP7011748.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            15519..17468
                     /locus_tag="IVN13_001251"
     CDS             15519..17468
                     /locus_tag="IVN13_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7011749.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            17471..21112
                     /locus_tag="IVN13_001252"
     CDS             17471..21112
                     /locus_tag="IVN13_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011750.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            21512..21850
                     /locus_tag="IVN13_001253"
     CDS             21512..21850
                     /locus_tag="IVN13_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011751.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            21960..22181
                     /locus_tag="IVN13_001254"
     CDS             21960..22181
                     /locus_tag="IVN13_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011752.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            22201..24357
                     /locus_tag="IVN13_001255"
     CDS             22201..24357
                     /locus_tag="IVN13_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011753.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            24369..24602
                     /locus_tag="IVN13_001256"
     CDS             24369..24602
                     /locus_tag="IVN13_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7011754.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            24592..25458
                     /locus_tag="IVN13_001257"
     CDS             24592..25458
                     /locus_tag="IVN13_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011755.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            25473..27443
                     /locus_tag="IVN13_001258"
     CDS             25473..27443
                     /locus_tag="IVN13_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011756.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            27610..27837
                     /locus_tag="IVN13_001259"
     CDS             27610..27837
                     /locus_tag="IVN13_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011757.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARVFFNEVKGMREQYEVVEA"
BASE COUNT         9989 a         4240 c         5028 g         8629 t
ORIGIN      
        1 acttttgttt cgtgttaagt ttctttacat tcctacgcac ctcgagtgta aaatttcttt
       61 attcctccca cgctccatgt tattttatat ttcctttttc atgttatttt tatccagaaa
      121 ccgatcaagt ttttacctat tcccaaaatc catttattcg ttattttttt cactgattaa
      181 gaataattat ttgatgctta aattagctaa aaaagcgatt tgttactgat ataatcatga
      241 tattgcagct gaattaggcg gaaagataaa atgttcgcaa aatcaaagta tcaattatct
      301 tgttttttta taaatttgta agtaaaaatc taaaaaagtt ttcttttaat tggtatatgt
      361 attaaaatca catttaatca cccataaagt atgcgctttc atttttttgg atgagattaa
      421 tatcaataaa taacgatgta aaaatcattt attatgctct tttataagga tattaattga
      481 tctaatttga aaaaaaatca aaatttaaat gttttaatat tacaaataaa atcatgttat
      541 tgtaaaatgg tataaaaaag ctaaatattc tatttttgtt attttattag caggaaaatt
      601 taaatagttt tacaagaatc cgtagaaata tattggtttt gccttcagat taatactgga
      661 tcatattaat ctgaatataa tatatgaact aataaatagc ttaacctcgc tattttgttt
      721 tttctgatca ctatctatac agcattagta aatagaattt taaaaataaa aaaattaaga
      781 caaaaaagta tcaacataaa catatacata tacacgttta tgttgattca cttaatttgt
      841 cctttaaaaa gcacttattt taatataatg ttattttata atcttattac aagctgattg
      901 ttaggcgttg tcctttcatt acatttatta acggttattt tattgtttaa tttattaatt
      961 atttttgatc taaaacttag cttaacaaat aaataattaa ccaaccgcca gataaaaatg
     1021 cgatcattgc taatacataa gcaaagataa aaatatgttc gatcagctta aaccctgatg
     1081 cgattaagca gaccaagtaa aatagaaatt ctcttacaga taatggtaat tttctaacta
     1141 gcatactcat aattaacacc ccaaagtaat attgtatttt gaacatttta catccaatac
     1201 agcatcaatc aaccattgcc aatcaaaaat acaaggcttc attgaagtat gtttttcaga
     1261 tgaaaagata taatataatt tcttcggaat aatattttca gttgttggga tcaagctaaa
     1321 tctaataccg tctaactcat agctaccagc tttctctcca ttaaaaattt ccttagcact
     1381 ttcttctaaa gaatcaaaat atttttcgac agcaatcttt gaagctttta ctattgaagc
     1441 atttcccttt tcgataaaat ctgcttttgt tttaccgaca ataagtaatt cttgatcagt
     1501 aaaatcttct aaaataatgg tagtgtaatt cttatttctt ctcaatcgca tcaggatcac
     1561 tccttaatat aatttatgtt tttataatat cataataaga taaatttgca agcatttatt
     1621 tacacctttt tatttacact ttattataat taattagact tttatgattg catttttttc
     1681 ttataaacag ttgcaactaa tagtaaatag gtgtaaacta ttttcgtacc gaaataccat
     1741 aaacacaaac agaaagaggt cataccatga aagaaccatt attgttttcc gttgacttag
     1801 ggaataaatc agtaaaagaa atgagtacac acgtttctcc actctcactt ccctctcgta
     1861 ttttgaacaa aaatatcatt aattcaggac gtttttctaa tataggttcc tcattaaaac
     1921 gatcactaaa cgtttctgaa tatagcctag taaacactac tgaaagctat tactttggga
     1981 aggatataca cgagctagga aaagatgact acgtgataga atcactaggt tttgggatca
     2041 gcagatacaa gcaggaaacc taccaaaata tcctatcttt ctctttagct aacctagcac
     2101 gtttagaaga tcaatctgat gacgttttaa ccgctaatct agttttaggt ctgccttcat
     2161 ccgactatac cgaaacaatc gtcaaatacg tgatgcaaca agccacacga caacattcag
     2221 ttgaagttga cggtaaagct tataatataa acgtagatac cgtttgggta ctgccacagc
     2281 cgattggaac actatataac ctcctacttg ctgatgatgg tagtgtacaa gatgaaacat
     2341 tgatcagtca gaagatcggt tttgttgata ttggcggtgg cacgaaactt tttgatgttg
     2401 tccaaaattt tgaaattgac gaaaacaatc gtgaacaaaa ggataccggc atttttactt
     2461 tatataatcg tatcgtaaat atgcttgacc ttccgatcag tcctaatgct ttccaacttg
     2521 aaaagattat ccgagaaggc aaagaaaaag gcgtatatct atttaagcca agcggaaatg
     2581 aaagttttga tatttcagaa gtagttgaaa gagaaataaa aacatacacc aatatggttg
     2641 ttgcagaagt tttagctaca ttcaaaaact tcacaacctt agataaagtt gtctttactg
     2701 gtggtggagc ttctattatc gacaaacaac ttgtaaaaga taaaattccg cataccttgt
     2761 ttgttgaaga cagtgaattt tctaatgtaa aaggctttta caagtttggt ttaactcaat
     2821 aaaacagcga aggagatcaa catgaataag ggaaaatcag agcgttatgt agttataatc
     2881 aatagagaaa aggttagcga gttaccttta gcagagtttc ttgaatccga atatgaaaca
     2941 ctaggcgtta aatactctca gacgatcaaa aaagctttga acctatatcg gcgtgtaaag
     3001 aacaatgaag ttacaattat tgaagcaggc aacgatagaa acgatagcaa agtagcagaa
     3061 gtaaagaata aaccaactga tcataaccct attcaacaag agcatccttc aacggttaca
     3121 tccgatcatt cagcaaagaa agaaaagcca atgctgaaag ggttcaatgc accaagtaac
     3181 aaagacttac tttaaaaata aaaaggcgag gttacaatac ctagcctttt tattatgtac
     3241 aaatataaac ataaacatat acgtatacat gtatataaac atgtatacgt atatatccat
     3301 aaacacgtat attaaattta gttaagctat acctaaccgc ttattccaat aaaaaaagtg
     3361 atcagcttta aactgatcac tcaccaaaaa cttcatcata caagttatct atcatttcac
     3421 caagtaaagc tgaatctgat cgaattccta acttttcgct gattttagat agtttctttc
     3481 tttgcgatgg ttgaagcgtg aattgataat ttttcttctt ttcagaacta tcacttttta
     3541 gtttagctac caaagaactt gttgtttctt taggtgtttg tttaggttct tcaactgctt
     3601 caaccacttc aggaacttct tctacttcag cttcagcgaa ttttttaaat ctattttctt
     3661 tcacttcaat cactccttat tcttgtgccg tatcaattat tttgttgtat agcgtaaacg
     3721 tgctttcaaa gaaatcctta ttcttacgat agatattctt atccttagcg aactcagcaa
     3781 tattcttacc ttctaagcta gttgcattaa gcaattcctt ctcttgtaca tatccaagat
     3841 aattagataa cgttggtacc agttctagga actcacgact tgaattagta ttatgtttga
     3901 tcctgctacc gatcgtagcg tatttagttt ccatcagtgt ttcaccagtc ttaatatcaa
     3961 tcaactgttt tgctagtgca tcaatatctt taccaagctt cagcaaagct tcaaaaccat
     4021 ctttagatgg atcagaaatg cctacgacaa gatcagatac agcccaagca ttttgagcta
     4081 gtagacctgc atcattatga gtatcaatca gaattagatc atacttctct ttaagctgtt
     4141 catagttaac aaccatccat ctaaacagta gggttctgtt gttaggcata tcacgcattt
     4201 gcatctcaac ttgttttaaa tcttcacctg ctactaaaat atccatgttc tcatttacag
     4261 ctaaaggttc agctgatcca tgaagatata aatgattgat tgtattctct cttttcgctt
     4321 tactgaaagc atcaggaaat cgttttgata ggttttgcga cttatcttca tcaatataca
     4381 gcaccttcat acctaaattt tcgacagcat atccacctag attgtagatt gttgttgttt
     4441 tacctgttcc acctttttca atgtttccag taatgattga caacttcttt tccatgatta
     4501 aacctccctt attaaccttt tgtaactcca ttatacacta taaacacgtt tatgtatatg
     4561 tttatataca taaacgtgtt tatatttata tttgctttta tattctacaa cataaacata
     4621 tatatgtata tgtttatgtt tatgtatata ttcatatata taactattgc atttgtcata
     4681 caatcgtagt acaatgtaat tgaaaaggag ttgataaact atgccagtaa taagtattcg
     4741 tgtatctaaa gaagaaaaag attttcttga aaaagttgct gaattcaacg gagataacat
     4801 ttctgaattt atgcgtagta attccctgca aacagcagaa acgttagtag actttgccac
     4861 ctacaaggaa ctaatgaaag agcatgagga aaacgatcaa agtatttccc acgaagatat
     4921 gctaaaagag ttaggcttat aagatgactt accaagtaag atatgaacgt gaagctcaaa
     4981 aagcgctaaa aaaaatggat aagtttcagg caaaggttat cctaaactgg atcgagaaaa
     5041 atcttgtagg cactgatgat ccaagaaaac atggtaaagg tctaacagct aataaatcag
     5101 gctactggcg atatagggta ggtgcctacc gtataatagc tgatattagc gagcatgaag
     5161 tgacaattct tatattgtct attggtcaca gaagtgaagt ttataagtaa cagacaagct
     5221 gataaggctt gtctgttttt ttgttaaccc aacaaaatca ctgtttacga tcccacttta
     5281 cctaaaaaag aacaataccc tcttgttatt atacacactg gtgtgtataa tataagtgta
     5341 aacaagctaa ttaactttca aaaggagcga ttttttatgg gatggtatgg tgcttattca
     5401 tcagcaaatg aagtgatcga agaaaagaaa gggtacgcca aaaatggttt ggaaatcgta
     5461 gataccaaag taacgaagac ctatggtatt atccttttca aagatgcaga aggaacctac
     5521 gatattgaca gcttcatctt tcgagatgaa atgtacaaac caatctactt cttaaatgac
     5581 agtcctgctt actttaagaa aattccgaaa aaatggttga aattctttgt cggtgatcaa
     5641 gaaaaaatgg ttaagcaaca actagataga attaaagaag aaaacaataa accaaacctt
     5701 gatgacttat tagttgcagg taaaacttac tcaatctggg atggatcgta tgtcgctgtt
     5761 tatcagtata aactcaaacg tagctttatt tttagactac aagatggatc actcactcgg
     5821 ttcactcgac tatcagcagg agatgttaag gaagtagcct aaaacttaaa tacataaaca
     5881 taaacgtgta tacgtttatg tttatgttta tgtatatgta tatgaatata aacgtattag
     5941 gtacgaacgt ttttaaatgc ttttctgggc tttgatttta gggtagggct aaaacatcca
     6001 ttgatccaaa acagctcaga aacgcattta aacgtatctg ataccaatat tacagaagga
     6061 gtgtatcgta tgattaacct atttctttta gtgacagcta gtacattatt ctttactgga
     6121 atatcactga taatttccta tttgatctat aagaaagccg aaacttacac aagcaaagca
     6181 accggactag ccgtttcctc ctttttaact ttgctgactt tttttgttac tctatcgttt
     6241 tcttttgttt agcacagctg atccatcagc aaaaaacctc tctttacatt atacacacta
     6301 gtgtgtataa tgtaattgta aacaagctaa ttaactttta ggaaggagtg acaatatgct
     6361 aacagctaca ttacagcaac ctactcacgt tgctattgca gaaagctata caattaatgc
     6421 aggggtcgtt aaacagctga tcaaggatcg agaattcaaa aagtttaaca gtcgtcaagc
     6481 gtataacaga cagttaaaga aagctattct aaagcacaaa aattccttca tagggaaatt
     6541 ggaaagtgtg gatgttaaaa ctgtttcaac ggcattttca gttgttgaag tgactattga
     6601 cggtcgcttg atttctctat ttgcttttgg aattaattga tagggagtgt ggactttgga
     6661 aggtaagact tttgcagaaa tttggactga atttattgag gtaccaactc gcattgatcc
     6721 gataacttct gaaaaggtat tggatgcaga cttctacaat ctcaaagcag gtactagcct
     6781 tgaatgcgtt tagatgtatt tagaagagca tttcaaaggc gtaacgctcg gtcacgtctt
     6841 ataccacaat ggtgccgagt gggatgcaat tggaaatatc catttttctt aatacaagaa
     6901 aggagaacac aatatgaatt ataccgatga agaacaaatc actttggaat acgttttcac
     6961 aaatgaaaaa ggggaggaaa ttgaaaaagt cttgatcatt tcatcaaaag acttggagaa
     7021 cgctttgact tatgaaccat acgaatcaac cgtaactgtc aatgaattct tagataccta
     7081 tacttctaat gatacttggg aaattatgaa tatttgcaac gctaataaca ttcaatttgc
     7141 tgaaaaaacc aataaatcat tttattagtt ttgcaataaa acatcctctt tttctattga
     7201 ttccatacac aacaatgtgt ataatagatt ttgtaaaggg agggtagcta tgcctaagac
     7261 agcaagagaa attgtaaaaa ttctcaaatc taacggcttc aaaaagactg gacaaactgg
     7321 cagtcatgca aaattttata attccaagac taaaagaacg gtcgttgttc cgatccacaa
     7381 tggtgatgta ccactaggaa cagaagctaa tatctggaag caagcagggc tttaacagcc
     7441 ttgccttcct atttggtatt aggcatacta tcctctctta cactagaaaa ggagcgagga
     7501 aatgaagaca aaaaatattt tttatccggc aatcttcgag aaagaaggag aagcttataa
     7561 catcgttttc cctgacttac cggaaatatc tacctttgga aatagtcttg aagaagcata
     7621 tttaaacgct aaggatgctt taggattagc gctatatagt gtaccagatg cagaatttcc
     7681 taaaccatca gcagtggaag aattaacttt gaaagccaat caagtggtag taatcatcca
     7741 actaaacatt aaacttttcc gaagaagctt aaatacaaaa acaattagaa aaaatgttag
     7801 tgttccggaa tggttagttc ttttagggaa ggagaaaaac ataaacttct ctcaactact
     7861 tcaaaaagca ctagaagaag agttgtttga aaagtaaaaa ggagaccgaa cctatgaaag
     7921 ctacacactt aaactatttc tctcgaacgt taatcaataa tctattaaaa gatacaactg
     7981 gcattagcgt tagtaccaaa cactttgcca atgatctaat tatttttgag gataataact
     8041 tgcgaattga gatgtttatt gaacaggcaa cccctaactc aatacctgaa ttctgctata
     8101 cgcacactga aaaaatgaaa catgcaaaat cagttaaaga tgttgaagca ttaagtgaat
     8161 cactaacgaa cgctttaaac ctcgttaaga aggcaaaatt gttatttgaa tttcaaaccg
     8221 actaccacaa tgagttgctt gctcaatctg aaattttaga ttaattgaaa caggagaaga
     8281 gaaagatgaa acaaacacaa acagaaccca ttaacgatac gcttgctgat caattagcag
     8341 gatattataa attatttgag atgagtaaag cacagcgctt gatccactat ctttggaaat
     8401 acaaaatttt gtttcttttt tgggaagcaa cactaacatt ctcagttctt tttttataag
     8461 tcagattgtt ataatcaaca cttagtttta gggagttttt tgcttatcta caccattttg
     8521 atgcttaatt tatataccta catcacactt gaagaacact tagtaattag cgatgcaggg
     8581 atcgagagaa acatcaaaag ggtatctttc aagatcacgt taaacaagcc taccgataaa
     8641 aagctctcag aagcgttctg agagcttttg acattttctt gtataaatta tccaaacaca
     8701 ttacaaagca cctaattcga gcttatatca agcaaatttg ctatttattc ctcaatacat
     8761 atctaaaaat tacttgatgg aaaactagat acatggtaag catgaaaaga aaatcaattt
     8821 gagcagatgc agtataatac atagcaattt aaaagctctc agaacgcttc tgagagcttt
     8881 taatatattc tttgataatt tgtctttctt aaatccatac gtcctaaaac aagctgtttt
     8941 tgcttaatat gatcatctga gagacatcaa atcaactttt ctctcaacaa atcattcaaa
     9001 gctttcgaca gattaatatt ctcttcagcc gctaaaacat ttaaatagtg ttccacagtc
     9061 actgattttc gatcgtaatt ttgcttgttt cgagctttag caactttcgt attagctttt
     9121 acgacaaata aattaacatt ctcagatcgc ttgattcttg catttaactc ttgaacaggc
     9181 gtaggcgatg gaatttcttt ttcttgcaac tctaaaagca ccaagtatcc ttccaatact
     9241 tcttgcgcca tctcaaaagc atcttctaaa gtttcaccat gcgtatataa gtctggaaaa
     9301 tctgggaatt taactgcata ttcatttcct ttccattcga atactgcaca aaaaaagtat
     9361 ttcatcgcat tgcacctctc taattaaaaa ataatttttg ctttttagtt tcaaaaaaag
     9421 aatcgcctac caaaaaaatt gataggcgat cacaagctaa ttaacctcta aaggagatta
     9481 ataccttgct cccaaggcac tacatatctt agcatacgtc tgaagcatct tcaaacgagt
     9541 aagatatatt ttgcattaac ctaatttttt ttctctataa ggtttttcat caaactcact
     9601 agaaatatta aactggacag caaaattgta atttctgcct tttctaactt tagcaaaatc
     9661 aatcttcagc attgattttt cattaatttc ctcaactgat tcccttaaaa cacgtctatc
     9721 aaaattcgat attgttttgt actcattttc tgttttcgta atctcacgaa ttttaactat
     9781 ttcaatcagg ggattcttat atatttcagc tttctcaaaa tctcctttat ttttggcgat
     9841 aagatatttt ttataataca tcgccaaata tttgtataac acaatgctat attttttttg
     9901 aaagcttgct aaatcttgaa aaacaaaata tggatatttc tttttgattg aaagaattag
     9961 gggaattact tcttgcgaga aattgaaagt tataatttgt tcttctaagt cccaatgaat
    10021 ttgatcaata gcagatactg tagcataagt cttatattct ttatctaagt tcgatatttc
    10081 ataaactgct tgtctctgaa gggtgttaac tatatttttt agttctgttt caagattttg
    10141 atcaacatcc atgtatttga ttattgcttt aacaggtaaa actatttccc ctgttttctt
    10201 taatttgtta ggagagatat aactgaacag caattgataa agttcatacg actttaaatc
    10261 cattttcatg tcaattgttg ctaagaactc ctcattgtac tttgtcagtt catctttttt
    10321 caaaagtttt tccttccttc acgcattttt tcagttgtta atcataaagg ttcacataag
    10381 gcaaataact cgtaattgat ttttcgagat atttaacagt attttttatt gtgtaatcct
    10441 ccttatgagt agcaacgtaa gataggtgat tgacaactga ttcaaatcct ctatctttgg
    10501 ataaacgatc ataaagcgga taaactttat tctgaagtcg gctcataagt ttaagatcgc
    10561 ataagtcggc agaactgatc aaaaagtttt tgattagtaa tttagtgtat ttgctattca
    10621 tagctttcaa gtacgattga ttcattttct ctgttttatc ttctgcgcct tttaaataag
    10681 cagggtctct ttgttcttgc ttataaggta ccggaactga catggtacgt gtaagcaaga
    10741 actttatgtt tgtataagta tgacccttct tgataagctc atagtcaatt ttaaaatcag
    10801 tgtatttatt cagttgggca atcggttctt taattacacg tctttcaaag tctcgaaaag
    10861 ctttatattt tttctcaata ctaaatttct ttcgtaaatc tgcaatggag aaaacaggat
    10921 tttttaatct atcaagtgat tttgtactcc atgttaagtt atctctattt gcttcataca
    10981 gattaaaatc tttagtgaat aattgaaaaa aactgatcgc atacttgctt gtcaagttaa
    11041 ctacttgatc aaatgtatat tgcgtaaaat gatcttctaa gttcgttaat aaaggcataa
    11101 ttgaactact aaatttaata gatactgcat cagaaaaatc attgaattca gtaatttcta
    11161 gcggtgagat gactttgtat tcagtcttac cgttaggttt agttctttga attttaatga
    11221 cagattgctg atgcaatttt tcaataactt gttttaatct tgaatgtttt gaagaagatg
    11281 aactagaaga catcaacgca aataaagcct ttttagatac tttgacggta ttattaaaag
    11341 gcggattttt agggtcaaga tgtcctatag cgtaaagata aattttttgt ggcatagcat
    11401 ccatttcaac aatgctatct acaagttgat tcgataaagt aacagttaaa tcaaactttt
    11461 ttagattgct ttcggtagtt tcaaatttcg ggtttacatt cttttctgtt attttcatac
    11521 gattgcctct tctttctaag cttggtacga gcttaaaaac ttggaactgg tacgattcca
    11581 ttgaataagt atagacctgt tttcgatacg ttacaataag aaatgtatcg taaatttata
    11641 acataactca atgtcagtaa aacacatttt attttttcca atcaaatttc attgaaaata
    11701 cagcagaaat cgctatttta gaacattttc aaaataaaaa tccttttcaa taaaatacga
    11761 aattataaaa attaccaaaa ttaaaaaaca aatatttttc tagtttttag aaataaattc
    11821 acatactttt tctttttttg gatatattcg gaataccaca tcgctttttg tccacgtttg
    11881 catcgctttt tgtccacatt cacatcgctt tttgtccatg ttcgcatcgc tttatgtcca
    11941 ccctaaaatc tatttaagat tgatataaag cctttttgga ggttgctaaa gttttaaaga
    12001 ttaaaatata tattataaat ataaataaaa ggggaagctg aagcttcctc cttttccttt
    12061 ttttcaatta ttcaaagtta cacaagcaaa ataaactcct agatagtgtt agtttttttt
    12121 aagatttttt atttttaaga ttttgaaata agagaggagt aatcctcctt tattaaatat
    12181 ctgttaatat atctgttaaa tatctgttaa tatatctgtt aggggaggct taatctcttg
    12241 ggagagtaag tattaaaggg gtataaaggt aacacttttg gacagtaaag gtaacaaaag
    12301 tggacagtaa gggtaacagt tttggacagc aaaggtaaca aaagtggaca tttgaggtat
    12361 cacactagga taagacgtta ccttcggttt tcttgatttg gaaggcgaaa aaatagaaaa
    12421 ttagattagt aacaaatctt tgttgactgt tacctttagt tgtgatttaa tttaggtaac
    12481 aagcagaaaa aattgtttcc tttaattagt accagattaa catgattaaa ggagcgcaaa
    12541 aagaagattg gagagtaatc aatgtctcaa gaagttaccg aaaagattat tgatagtgat
    12601 gtagatttag tatcagaaca aacaaatgca attgtgcgtt ataacaatgg tctgaataca
    12661 acagctttgc gaggatttaa gcctgttgaa atggatgtgt tttgggctat tttatcaaaa
    12721 atgaaacgtc aaggaacagc agaagtaact tttgatttca acctctttcg ttatttggtc
    12781 aaatacgaca gaagagaaaa ggacagtttt tattctgtgt taaaagattt gagtgacaaa
    12841 ctaggtaatt taacctaccg ttttgaagat tcagacgtct atgaacagct gtggcttttt
    12901 caaaaattcc ggatagataa acagaaggaa acagttacaa ttaaggcaaa tgaggaattt
    12961 gaatttatct taaactcaat tggcaaaaac tttactcgtt ttgaacttga taattttaca
    13021 aaactaagaa gtagctacac aaaagaattg tatcgtcatc tcatgcaata cagaaataaa
    13081 gagtggcgaa atggttattg ggtagtcaaa gttgaagatt ttagaagagc attaagtata
    13141 ccttcaagtt atagaatgac aaatattgat aaaagaatac tagagcaggc gaaagaagaa
    13201 tttttagctc ctgatgaaaa cggaatacag gtatttgaaa cttttgatta tgaaaaaatc
    13261 tatgcaaaga aaggaagaag agttgatcgg ctcagattca cttttagtga gccagaatca
    13321 aacttgccaa ccatttcaat gcataattgg ttagaagaag attgagaaga agcaggttgt
    13381 aattttaata caacctgctt ctttaaaaaa gtatgggtaa cacttttgga caagtcaaga
    13441 aaaaaaacaa tttttcttaa tttgttgatc gactaaaggt aacacttttg gacaacacag
    13501 aataaaaagt ccggtgatat cagttttggt aggaaacatc ttaatatctt tgtgagattg
    13561 aaggtaacat ttttggacaa gtcaactatt tgttaaaagt taatatagag taattagata
    13621 agttttttct tttcgtctga taaatggata atattagtta ttttattgct aattagtctt
    13681 tcatgatgac tgaaatagct aaaataaata tctcgttgga taattgctga tactttaagg
    13741 gatatctaaa tttgctgatt gatttattta tgttattatg atattataat aacataaatg
    13801 tgttacgccc gaacctcatt ttaaaaaaat gaaggggtga gacaatgaat gctattaatt
    13861 ggcatgaatt tgttaactat atggagagct tactagatga tctaaaaagt tttagtattg
    13921 ctgatgcagt aaagcatggg ttaccagtta gtacaacgat gaaattacga ggattgaaaa
    13981 ttccagatga aattattaag ctgagaacta aaacaattat tgaaaccgca aaagtcgttg
    14041 acaatatgaa ccatgaacta aaaggcaaga aaacaactta cgagaaagat tttgaacgtt
    14101 ttttaaaatc tgttattgga aaatatctag gtaaaaaatc tttcagtgaa ttgagagatg
    14161 aagagattgt acctactcca atggttagca aaatccgaaa aaacaaagcc gaggggaatt
    14221 actatcttgg tacaatagca aaaatcattg attatttgga aaataaagag caaaagaaag
    14281 gaacgaagaa aggaagataa tttttgtgtt aaatattaat ggggaagaat ataccgaaat
    14341 gattcaaaat cccgaaccta gaattaccga aaaaacgtct gattgtttgc tcttttatga
    14401 aagggggcag acaaactaat ggaaaaaaac gtcaattcaa catctaccaa agatcaatac
    14461 agtattattg gagggttgct tggtgataag gataagagaa cacgtcacgg attacgcatt
    14521 atgcttgcaa cttttctagg agtgcttttt ttattaacag gcttgggtag ctacatttat
    14581 caattaggaa actataatta ttatctcgtg aacaatacaa cacctatagg ctcttcattg
    14641 gagtttgctc gatcaggagc agagatcact atttcagatg tgtggacaga tcaaaacaga
    14701 gatgtaacag tagtaaaact cggttactcg caaaacgcta gaaagttgtt atctacgcaa
    14761 ggaaaaaact acaacttata tatggcaacg aaagaaaaag atcgtcctaa aaagcttgaa
    14821 atgagctatg ggatgttaag tactgatggg gatgctttct tattcctaaa aggtaaatta
    14881 gatgaaagag cttatcaagt ttttattgca aaccaagtat cactaatttc tggtgatgat
    14941 acagctgacg atgataccaa agtcaatgtg aatgaagcag attcagttac aaaagcactt
    15001 tctcaatatt ctatgaatga tgttaataat gaaggagtaa tttcttttgg gaaaaaagca
    15061 aaacctgtta atactcctga taatatcaat ttcagaataa atccatattc agattcaact
    15121 aatgtctatc aaggaagctt tcttaccaaa gatggcgata ttgattattc aaaagttgta
    15181 gcagttacaa gtaagaataa tgttgtaagt aagttagaag atcagttaaa tgcttcaaat
    15241 actaacatca agaaaattga agctacaaaa gctgagtatc tggaacgttt acaacaaaat
    15301 cctgacgata aagattcgca agaaagtatt cgtcaactag acagtcgaat ggagaccgaa
    15361 attaaagcaa tcaatgatac acaaaaattg attgatcaat acaaaaatgc tgactttaac
    15421 aaagattctt ttggagacat gaagacgaca tttaagtacc taactctgac tgactagaag
    15481 taaattattc ttattttaga ggaagtgaac acaaacagat ggaaaatagt accgagtttt
    15541 ttaagaatat tcaaactgaa tttaatcatg ctctcacacc aatgaaacca ccgaaacata
    15601 agttagtggg acgtgatgac gaactccatg agttgtcact gatcttgaat agaccggaaa
    15661 caccgcaagc tatcttgtta ggtgaagccg gaacaggtaa aacagcactt gcaagtgaat
    15721 gggctgaaag acagcgacaa gatggaaaga aagtacaagt atacaaatta tcaatcggac
    15781 aattgcgtgt tgggggagat gatgtcctcc aaacacgttt agaaacatta attccaagat
    15841 tgaaagaata tgagcgaatt ttaaaatctg aggacaaaga aaatgaattt gtcctcttca
    15901 ttgacgagtt tcataaagtt gttagctctt ttggtagtgg caataaaatt gggggtgatt
    15961 cgctaaaaga accgttagca aatgaatcac ttcaattgat taccgctact acaagaaaag
    16021 aatatgaaaa ctatatcaaa agagatgatg ccttagggag acggttacaa ccgttagaat
    16081 tgaatgaact aaaaagcgat gttgtcgtcg atattttgag acaatggcta aatacatcct
    16141 ctgttaaaga tgatcctcag gcttctttta aattatcgca aaggattcct gatgaattac
    16201 ttcaagaaat cgtacaagca aacaagcttt atcgtgagga tttgtccgaa ccggctaaat
    16261 caattgccgt aatggaggat ttgattgcaa aagatcgagt agacggaaat gcttataatc
    16321 gagaaagtct attgcaaata tttggtaagt ataatattca gctagattct gattttgata
    16381 tggaaaaact tcaagaggaa ttaaagtcca taaaagggca accgttagca gtttatacta
    16441 taacaatgtt gtttaaacgt atgaagcttg gatttgatcg ttttaaaggt cgaaagcaac
    16501 caaaagcaac tgctctgttt acaggatcaa caggggtcgg taaaactgca atggctaaag
    16561 ccgtggcaag aggactttat ggcgatgaaa ataaagttct cttagttaat atgccggact
    16621 acaaaacaaa agatgatgca atgcgtttta gacgactcat aggtactcat gtaaggcatg
    16681 atccttctgc gattattgtg tgtgatgaat atgagaaagc tggtgtagaa acactcgatg
    16741 cctttttatc aattttagat gaaggtagga taagttttac tgaaagagga tgggatgatc
    16801 gagaagttca cgacactgtt tcattgtcaa atacaatttt atttgcgaca acaaatgcag
    16861 gtgcggagat gttcgattcg ttaaacagat atagctcact atctcgtgtt acaaacaagg
    16921 aaaattctaa tgaatatagc actgaactaa aaagagaata ccgaaaagtc gcatcaggga
    16981 tcaaacaagc cttgattggt gaaggtctaa agcctgaagt tattaaccga tttggacgaa
    17041 tagttccatt ttgggcttta tccgaacaaa ctcttctgga aattgcttat aatgaaatgt
    17101 tgaagagtgc tgaattagta cgaaaacatt taaatataga tttacgtctt ccacctagga
    17161 aagaaagagt atatggtcaa aatttttatg cagacgatct ctccatgcac ttagtttttg
    17221 acatggcttc acaagaggat tctaaatcag ggggtgcaag gaatatcttg catttagttg
    17281 aatctgagtt tgaagaagcg attactgaag caataattac ttatccaaac gcaaaaatat
    17341 tcgatgttaa gttgagtgaa gccggagcgt ttgaaggaaa aggtgattct aatgctgaaa
    17401 ttattgcaaa gccttccgtt gctgaaagta aagaaaacaa gattgtattt tcgattggag
    17461 gggcgtagct atgaaattca agaaatattt actacaaaac cttagaataa aacgatgggt
    17521 cgtaagaggt ctagttattg gaggagtatt agcagttact ttaggcgctc cttttggaat
    17581 gaatgatcca actcctgaca atcaagatag caaacaagaa acagttataa ataagaacgt
    17641 tgcatatgcg gaaagtaaac cggcagaaca aggaatgtgg acactatatg ctcgtatcct
    17701 aatgggagat aaagaagtta atgatagtgc cgctaaaact ggacaaatga atttgttagg
    17761 acaaggtggt atcagtgccg atttccctta ttctgacatt tctaaacaag gtaattcgat
    17821 tgcagggggt aatgcaggta ctcaactagc atctacttta gcaacttata gttactacgg
    17881 atatattgaa acagtctcag gaaatgctat tgccagtcaa gcatcatcca ttctaggaag
    17941 tattggtagg acgattggta gttttatcgg atcaattagt ttaatccttt ctaatattgc
    18001 tgaatccata aatgaactcc ttggaaaatt tttcatgagt atgaatatat tttctcttat
    18061 ggggtgggga gaaggtggat cggatgtatc taatccggta tctaaagcat taactagctt
    18121 gcttaacaat ttgggattaa ataatgaatt atttaaagcg atagcaagct tatctatgct
    18181 ctttttcaca gcaatactag gttatcaagg aatgaaatta ttgtcgaaag atggtgttaa
    18241 ctcgaaggga ttatcaccag tacttgaaaa atggattgtt cgtatttttg tattgtttat
    18301 attcttacca gtggcaggat cgctattggg agtttatatc aacggcatcc agtcagcaaa
    18361 aaaagatgga gatataccaa caaatgttgt ttctgactac atcattaaca caagactttg
    18421 ggcttctact caaaatcttg ctccatcatc tgatactggt ggtgatgtac ctgacggaaa
    18481 gcctacatcc gaaatcaagt tcgttgatac agattatgaa ccatctaaaa aatctgcaat
    18541 gataagagcg atcaatacta gcagttatgc cagacaaaat aatgatctag attctaattc
    18601 agtcggtatg gctctattaa aagaatggtc atcgaatgat actttcaata ttaatactta
    18661 tgccggtgat gttaatgtcg ccgaaaatga tgcttatatg ggaagagcgc ctgctgtttg
    18721 gacaggctat aatttagggg atggaaaaga aggaagtgca attaagaaac caaccaattt
    18781 aaatggatat atttggtcag caaatcaaaa tgtgacagac gacaatcgca agcctgatgg
    18841 aaaaggaaca gcgcataact ttgatgctac tcaaccttgg ggcgttgcta acaatgcaac
    18901 ttttagcaca cagtcggtag ctttattgct ccaatcggca cttgatacat cgggagctaa
    18961 gttttatgcg tataatatcg ctccttcagg aattcaaggc agtgctaaga atatgtcaac
    19021 agtaaaaaca gaatggcgta cagcatcctt agttggtcat ggagctatgg gtaagtttgg
    19081 ctcgtggcta gcgatgaacg ctgaatcaat cgttttagca gttctttact tagcatgtgc
    19141 ttgggcgatg ttcaatgtga atatcgtgaa agcatttaaa cgtgaattta ttcggatttg
    19201 ggaagtagtt ctaacaggat cacttgcatc aggagcaggt gtgatggcaa tttcattagg
    19261 tgttgttaca acatctctga tcggactaca attacctatc tttctgatta cttttattcg
    19321 tgcatttggt gatactgctg tgggattcat tcctaataca ttagttggcg atggcgtagc
    19381 aaatattgtt cgatctgcta tgattttagg tgtaggatat cttttagtct ttaaaaaagg
    19441 cgaaaaagta aataatttga ttaatatgct tattggctta ccacttgaag tagcgtttgg
    19501 gtttgatgaa cgtgctaagt tattatttgg taatagaaat gatgctgtgc gagttgtgga
    19561 cggcgtttct aaagcaatca agaaaagcca aggagctaaa aatccctatt atgatggtgg
    19621 caacagctca gtaaaagata tggttgcagg aggtcttgga gcagttggag cgcaaaaaat
    19681 ggctgaacgt ttgaacaata tgggagatag caatgctgat gctaagtcca ttgctgataa
    19741 agtaattcct gaaacaccag aaggaatgtc attcaacggt ggcggagttg gttccacaat
    19801 caataatacc tctttcaaag atttagttcg tcaagatcaa ctacgtaata actcccaaca
    19861 atatattgat catttaggac gtatggttga tgaaaacgga aactttgtcg atgaaaatgg
    19921 tgaaatatta aaagatagta aatacggtaa agatgctaaa ggcaacttta ttgacgaaaa
    19981 agggcgtttg gtcgatgaag atggaaacct tatagatact aatggtaaac cattggaaga
    20041 tagtgaagat ggtaatattg gtatggatag cgaaggacgt ttaattgatg aaaaaggtcg
    20101 cttagtcgat gaagatggaa atcttgttga cgaaaatgga cgcaggttgc taaatggtga
    20161 ttttggtcat gatagtaacg gacgtttagt tgacgaaaaa ggtcgtttag ttgatgaaga
    20221 tggaaatctt gttgatgagt ttggtaataa acttggtaaa cacagcgatg gatataatgt
    20281 gaatggagat ttagtcgatg aggatgggaa tatcattgag acgagagatg cgcttgtggc
    20341 agatcgaaat agagaactcg aacaacaacg gcatacgcca attgctgttg caattcctga
    20401 gaatgaagaa gacgagatta ctttagatca aactcatcaa gaactgagga atgaaatgag
    20461 tggtgaggac gtgtcagaca gagtgcatcg ggaattagga gatggagcat ctggtgaaag
    20521 tcagtcaaga gagcttaaca gcaactttga taatagtgct attcggaatg acacagtact
    20581 taaagacaga gaatcgctaa caaaagaaac ggataaagaa caatcagatg tgaagaaaaa
    20641 gttcattcct actcctaatg ttaatcaaac agataaatat gaaacaaata ttggatcagg
    20701 aatgtcgaaa gacaatcaat caattagaga acctgaactg acaaataaaa ataatagaac
    20761 aaataattct aaatctactt cagataattc agggaaatct aatcaatcaa ccaagaaaac
    20821 atcaacagct aaatcagccg ttgaatatct atcaaaaact ggtaaaaatg tttacacctc
    20881 agcagataaa gctttagcaa aagctccaaa aggtagtcta gcagggataa cctatgaagc
    20941 taaaaacgaa gctattaaaa taggtaaagg aacggctaag gttgccaagg ctactgctac
    21001 aggagcatcc aaaactggta aagtagtgaa gaaatctacc atgtcagtca aggactatat
    21061 tgtaaaatac ggtaatcaaa aatcaactga aaataagtct gaagacgagt aaaaagaagg
    21121 agcattgatt tgctccttct ttttactttg atttctaact gaagatgtca aggaaaattt
    21181 ctatttatgc aaaaaaatta ttttaaattg tctataaatg aattaaatat ttactgttca
    21241 aagttgtata attaaaaagg gcttatgctg gtaaagaaca gaattattat ctaaacaaaa
    21301 acttgataaa ccagcatttt tagccaataa aaaaagtgaa ataatttaat ggaaaatctc
    21361 ttttaatcat cgaaaattta tgttattatg ataacataaa gatgtaaata tcgagacgtc
    21421 tttttcttag ttagatcatt gacaagtgaa caaataagat ttgtcgatga tataattaac
    21481 ttattaaaaa tattaaaaag gaggttttta tatgttcgat tctattaaaa atttggttag
    21541 cggaatgacg cctgatatga aaactttagt acaagttgtt ttgtatttct tggcattcgt
    21601 tttttcgttg aaaccaggat tcactgcaat gaaatttttt ggtgatgcaa aatggggaga
    21661 tggattaaaa tatgttgggg cgattattgc aatctttatt attcctgtaa ttacaaccgt
    21721 tatgctttac acaatgggta ctaaaacagg taatgatttg aaccaaaaag ctaatatcat
    21781 atcagcactt attccagttg ttggtatcta tctagtttca aaatttccaa aaaatgaact
    21841 gttaggttaa aagtgaattg aacaaatttg gataatatgc cgatttgttc aatttacttt
    21901 ttaccaaaaa actacgctcc aagcgtagtt tagaagtgag ggaagaaagt aaatgtaaga
    21961 tggtttttga tagagaaaaa atgttagctc acgccaacga agtattaatg agcagtctga
    22021 aaggtaccga actggcaaag ataatgaata tgaatgttaa ccagttctat gattatcgaa
    22081 atgggagtaa aaagattgaa aaagccagac ttgaaacgct tattaaattc gaaaaagcat
    22141 atgtatatat gctagataaa cagaaaagaa ctattgactg aaaaaaggag ttttacaata
    22201 atggcaaaat tcaaagatat taccgaatct attaattatg tggcagaaac agttcctgta
    22261 gttgcactct ctttgaagcc acaattactc gaagtgtaca aaaaagaaga aaaagaagcg
    22321 aaggcaaaaa aagctaaagc aaaggtatgg aaagaattcg atgcgttgct gaccaaagat
    22381 ttaccaaaga aaaattatat ggtaattgaa gatgtacaaa agctcaacag tgagggagtt
    22441 atagatgagg atgatgctat tctcgattct gagaagtatg tcgttctatt gaagtcaagt
    22501 ttgaattatg tagctgatct accagaaaaa tttaaagaga agctattagc aggtgtccgt
    22561 tttatggata gtagtggtca attattagtt attccggatg atcctaatgt gtttgaatca
    22621 gaagttgaag aagctacaga cttctatagc gcagtagagc aattagaagt agctactaat
    22681 ggattagaac gaattgctga gaccttcaac tcttctgaag aagttgaatt tgaagccgta
    22741 gcagaaaatg taccagatga aaaagaagct ctttttgtgg atgataacaa accaaccgaa
    22801 gcagaaaacc aagaagtaag catagaagaa acacaaatac aaacagaaac ctaccaacaa
    22861 ccaaaaattg atgaaaaacc cgaatcccaa agtgacaaag aaaattatgt ttatgaagct
    22921 gaaaaaatca catctgaaag tgatgtaccg aaaaacttag atgctgattt tttagaaaaa
    22981 aattttgaac agatcattgc tgacaaattt caacctgtag ttttagatga gattaatgac
    23041 cctgtcgatt taaacgagga gatgctatcc atacctgagt tgaaaccatt gctgttttta
    23101 gctaaagaga gtatgaacgc tcgcatctct attgctaata ataaaatcaa cagtgaatta
    23161 aataagacga aggagcaatt gatcctcaaa ggaaagcaaa tgttagctga tgagcttttg
    23221 cggatagagg aagcaacctc attaaatggt caaaatcagt ttacaaatac actatcggtt
    23281 gctaaagaga gctacgaagc agaagcatct aaaattgatg atttagtgat ggaaaaatcc
    23341 aatatcattc gtaaagatta tgatatggcg aaacaggctt atttggatgc tgaactaaag
    23401 aaactagagc aacggtatga tgctgagcat ttaccagaag ttaaacaaca agaagatgaa
    23461 tatagaaaag caactgaaga aagtcttgat caacagtacc aagaagcaat tgaatctatt
    23521 aaaaacgatg cattgaaaca aagccaagaa gcaaaagaag ttgtggtgga acgaattatc
    23581 aattcattga aaccagaaat tgaaaaatca ttaacagacg tgcaagaaaa aactttagag
    23641 attgtcgaaa aaacaaagtc agaaaatgaa gctgagttcc atgatacaaa agagaaaaca
    23701 acagatgtgg ttgtcaaata ttttgcgaac aaagaagaag ttgaaaagca ggtagctaac
    23761 aacacgaaag aattagaaac aaaacttgta gagcaacaaa aaactatcga agaatataaa
    23821 gatcgaatga aagaggtcaa agatttagca gagggttata aattaaatag cgaaaagttg
    23881 gcagaacaaa gtgaagcttt aagagaagac ttatctcgtt cccgaagtga tctagctcaa
    23941 atggcagaaa aaacgaaatc attagcagtt gctacagaaa atcaatcaag ttcaaaacgt
    24001 gattttacaa gtaaaaagat ggctactatc atttctgtcg ctgcaatggc ttgcggaacg
    24061 atcttgggat cgtcctactt tattagtcat aataatcaac aagcacaggc tcaaactcaa
    24121 acatctattt cgagagtgga aaattcgatt gggacaaata cgaactctgc ttcaacaaat
    24181 agtagctatg aagcaacacc aactacagag ttcaaagttg ggaaagatgt tgtcatcagt
    24241 attgatggga aaacaactaa ggctgaagtg atttctattg atggagaaaa agcagttgtg
    24301 aaaactccag acggaaatca gtacaaaatt caagctcact tatctgataa taactaagga
    24361 gggctattat gcgagaagaa aaaatagttt atccagtaat tcaaaacaat caagaaaaca
    24421 tgacctcttc tcagcactta caggaagcac agctcgtgta tcaggtaaat gaactaccac
    24481 aaacgaacga gacaatgagc aagtcaatat ctttaattgg cttgcttatt gttgcaatag
    24541 tgataactgt aggtgtttac cgtaagttga acgtgaaagg aagtatttct gatggcagat
    24601 aactcaaact atggatttaa acataactta catcaaaaac cttctgaaac aattgtcaga
    24661 gttgaatggc ttgaaaagca tgtcaatagc acctattctg ttacagcaaa aagattaatt
    24721 gtatcagcta tagttgttgc agtcgttgtt cttggatcaa ttgtttactt agtttctgat
    24781 acgattaaac tgaaatttat ttttgttcca acgttattaa tgataattgg atttcttatt
    24841 ctaattagcc agtatatgtt tattgatcca tctagtggtt cattgatttc tggttctatt
    24901 agactgttca aatcagtagt taattcagca agagtatcta tggggaaaaa gaaaccattt
    24961 cgaggcacag agattttaaa agtgaaacaa gatggaacaa tcgtatatcg agatgggagt
    25021 gtagcattaa tgttcaatct agatggggct acatcactaa ccgcttttcc agaagaagtt
    25081 atggctcagg aatcgaaatc aatacaatat caaaatacca gagatcgttc cttagctgaa
    25141 atcaaaatca cttcatctca acctcagaat acggaaagac aaactcgaaa tatgaaaata
    25201 caaaaacgaa atgcaaggag taacaaagca gtctttgaca taatagatca gcaagaacga
    25261 tttgtatcag aatatattga tggtgtgaaa acaacagtaa tccaatatct tatcttgaaa
    25321 tctcaatcta agatggtatt ggatgaaggt gtggaacgtt tagaaaacta tacgagtaga
    25381 ggactttatt atagtgtcac tcagttgtca gctaaacaaa caaaagaaat attatctgat
    25441 atttatgcac tgaaatagag gtgagagatt aaatggaaat tatgggtaga tcacaactat
    25501 taaatttcaa aaaaaagaga aaaactaaaa aagaacaaaa aactagaaat tcaaaggtag
    25561 tgaataatac ctttgttagt gatttaacat ctttagttgg ttatcaattt acaccatctt
    25621 acattaggac aggtaagcgg tggggaacaa ttgtgaaatt agtgaatact tatggaatga
    25681 atagaaatta tggatttggt tggatggtta aagctattcc tgaagtgaga gtagaagggg
    25741 taaaagccta tctaattgaa gcagataagc cagtgtcaca aaaagatcaa caagaaataa
    25801 tgcacgagaa tgttggggga tcaacgaata gttatgaaaa taatttagaa ggaaaaaatt
    25861 cagatgctga aattagcaaa actgttatgc ggattatgga tttgaataca gcatctttac
    25921 tagacagcat ggcaaataaa attattgatt ggaagctaga aatcctatta gtatccgatt
    25981 ctcctaataa aatcagcgaa cagctaatta aactccaacg gttgtatgat gaaaaaatgt
    26041 atggattaaa gcttatgtca tatggaggag atcaacgtga tctgtttgat agacttttaa
    26101 gtgaaccaat cgggaatgtc tatgagtata cgtctatgag ttcggatttt gcaggaaatg
    26161 atcatgcaat tcgcaaaggt ttagatgatg cgaatggatg ggctatcggc actatgtcta
    26221 ctagctacgc aagtggtgta gcttttatgg cactggatga ttcttttaca agtaaagttt
    26281 tagtggcatc acacaaaaac gctacaatta aaggctatga tgaaaaaatc tcagcatcat
    26341 cactttgggg gcaattgatt gctaataatg caatggcaaa tcataaacga acttttcata
    26401 ttgtcttaaa taaatttgat tactttggtg atccaactgg tagaacattt gttgccaaac
    26461 cttcgttaaa cagtgtaata gaaaaggtta atttggctga agaaggtctt aactctttgg
    26521 agctttttgg taatcaaaag gacattggtg atttatatag acggaatctc gataaactaa
    26581 ctcagatttt ttatttgtta gcaggcagaa cattagattc tgcaactaga gttttattga
    26641 aaaaagaatt aggtaacttt tatataagtc agaaactttg ggatattaat tatgaagaac
    26701 gttcaaaact tttggtgaac agtagtgata caatgccagt tcttggggat tttattagct
    26761 atttgactaa tgctgaaaca aaagccaaaa aatctggtac tgaaaaagaa gtcgatcgta
    26821 ttattgtatt aaaagacaca ttgagaacag cattagacag cttctctagg atgtttaatc
    26881 aaagaacgac actgaaagaa atcggtaaaa gtgacaaatt acagttttac tataacttag
    26941 gtgatctaag agcagaaacc aatgttatgg aagctcaatt tttaaatacc tttgatttca
    27001 tcacatcaca agcaaaaaaa gatgatattg ttatgattca cggcttcgat aagttatcta
    27061 ttgaaaccgt tgggctaatt aaaaaaagat taacaatgct tgaagaagat ggaattcgtc
    27121 ttgcctttct atttgataca atcggttcag gagaagacaa tgaatacaaa gaagatattg
    27181 aaacagctga tatattcaat acagatggaa aaatttacca aagttttgaa agtgatttta
    27241 attacactat cattggaaag atgaccagtg gagagttaat gaagtatgaa aaaaaagttg
    27301 ctcctgctag aaaaggagaa gatgatcaaa aactttctgt tgatttgaga cagtatttga
    27361 caacaggaag aagtaataaa tttcagatca ggagaccagt ggatctgact aataattttg
    27421 ttattggaga gtttattctt taagttttat ctttgatttt gagtgtataa ggaaaggata
    27481 agaaaaaaca tgtatatttg agactaaaaa cagtcttaga gaacatggta cgttttgtga
    27541 aaagagaact atagaaattt cataaagtga aaccttattg agtgaaataa ttatagtgga
    27601 ggttaaataa tgaaaccaaa cataaaaatt ttagatcgaa tatttctagg tagagacaca
    27661 gaagtaatcc taattcaaca tgaagaagga tttgaagtta gtataggtat tcagaaactt
    27721 caaaaacctc attattgtaa tcagttgtat aaaaacttta ctgatgaaga aaaagcgaga
    27781 gtttttttta atgaagtcaa aggtatgaga gaacagtatg aggtggttga agcatgaata
    27841 agttaccaga aaagaaccgt gtatttagat tgaaaggtga tctcag
//
LOCUS       DACCKU010000031        27761 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.033, whole genome shotgun sequence.
ACCESSION   DACCKU010000031 DACCKU010000000
VERSION     DACCKU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.033"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            324..1985
                     /locus_tag="IVN13_001260"
     CDS             324..1985
                     /locus_tag="IVN13_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7011758.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2055..2402)
                     /locus_tag="IVN13_001261"
     CDS             complement(2055..2402)
                     /locus_tag="IVN13_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP7011759.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2486..3427
                     /locus_tag="IVN13_001262"
     CDS             2486..3427
                     /locus_tag="IVN13_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011760.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3428..4471
                     /locus_tag="IVN13_001263"
     CDS             3428..4471
                     /locus_tag="IVN13_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7011761.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4477..5547
                     /locus_tag="IVN13_001264"
     CDS             4477..5547
                     /locus_tag="IVN13_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011762.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5537..6490
                     /locus_tag="IVN13_001265"
     CDS             5537..6490
                     /locus_tag="IVN13_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7011763.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6502..7512
                     /locus_tag="IVN13_001266"
     CDS             6502..7512
                     /locus_tag="IVN13_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7011764.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7797..8618)
                     /locus_tag="IVN13_001267"
     CDS             complement(7797..8618)
                     /locus_tag="IVN13_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP7011765.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8715..8993)
                     /locus_tag="IVN13_001268"
     CDS             complement(8715..8993)
                     /locus_tag="IVN13_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011766.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9261..11270
                     /gene="metG"
                     /locus_tag="IVN13_001269"
     CDS             9261..11270
                     /gene="metG"
                     /locus_tag="IVN13_001269"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP7011767.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11749..12336)
                     /locus_tag="IVN13_001270"
     CDS             complement(11749..12336)
                     /locus_tag="IVN13_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011768.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12511..13833
                     /locus_tag="IVN13_001271"
     CDS             12511..13833
                     /locus_tag="IVN13_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7011769.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13988..14764
                     /locus_tag="IVN13_001272"
     CDS             13988..14764
                     /locus_tag="IVN13_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7011770.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14757..15326
                     /gene="rnmV"
                     /locus_tag="IVN13_001273"
     CDS             14757..15326
                     /gene="rnmV"
                     /locus_tag="IVN13_001273"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP7011771.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15319..16203
                     /gene="rsmA"
                     /locus_tag="IVN13_001274"
     CDS             15319..16203
                     /gene="rsmA"
                     /locus_tag="IVN13_001274"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP7011772.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16654..17283
                     /locus_tag="IVN13_001275"
     CDS             16654..17283
                     /locus_tag="IVN13_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011773.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IVN13_001276"
     CDS             complement(17407..18516)
                     /gene="ugpC"
                     /locus_tag="IVN13_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP7011774.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18725..19147
                     /gene="mgsA"
                     /locus_tag="IVN13_001277"
     CDS             18725..19147
                     /gene="mgsA"
                     /locus_tag="IVN13_001277"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP7011775.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19470..20006
                     /locus_tag="IVN13_001278"
     CDS             19470..20006
                     /locus_tag="IVN13_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP7011776.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20046..21809)
                     /locus_tag="IVN13_001279"
     CDS             complement(20046..21809)
                     /locus_tag="IVN13_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011777.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKNIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21812..23527)
                     /locus_tag="IVN13_001280"
     CDS             complement(21812..23527)
                     /locus_tag="IVN13_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011778.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSIPEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24306..24530)
                     /locus_tag="IVN13_001281"
     CDS             complement(24306..24530)
                     /locus_tag="IVN13_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011779.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24730..27498
                     /locus_tag="IVN13_001282"
     CDS             24730..27498
                     /locus_tag="IVN13_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7011780.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27579..>27761
                     /locus_tag="IVN13_001283"
     CDS             27579..>27761
                     /locus_tag="IVN13_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7011781.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEII"
BASE COUNT         9050 a         4997 c         5582 g         8132 t
ORIGIN      
        1 aaactggctt ttgaacaccg tttattcaga taagggactg tgacaagact tttgtcacag
       61 tttctttttg tttattcttt tttatctttg tatggcaata atagacaata taacgaaaat
      121 tatttaatgg cttttggaag accatggaaa accttgtaca aagctatttt ttctgaaaaa
      181 ttccttgaaa cctcacgaaa aaatcattat tttatgaaaa agacaaaaaa aagtgttgat
      241 tttttaataa ttattcatta aactcttaaa caacttcaga aaatattaat tattctgaaa
      301 attccaataa aggggtacgt aaaatgaaaa agaaatttac atttggtttg gttgcggtat
      361 gcggccttgt acttgctgga tgttatggcg gcagttcagc cacaagcagt agttcagcaa
      421 aaagcggttc agcagatgga ggaggcgtat ttaacttagt cgtgcctcaa gaaatgccta
      481 cagctgactt atcagttgca acagatacaa tcagttttac agcattgaat aacgtttatg
      541 aaggaattta ccgtcttgat gaagacagca aaccacagcc ggcaggagct agcgagttgg
      601 cagaagtcag tgaagacgga ttaacttaca aaatcaaatt acgagaagac gcaaaatggt
      661 caaatggtga tcctgttaca gcagcggatt acgtttatgg ctggcaacga acagtagacc
      721 cagctacagc ttctgaatat gcttacctgt atgcgccagt tgaaaatgca gaagacatta
      781 cagctggaaa gaaagataaa tcagaactag ggatcaaagc agttggtgac tatgaattag
      841 aaatcaaatt gacgaaacaa acaccatatt tccaatatct gcttgctttc ccatcatttt
      901 tcccacaaaa acaatccgta gtggaagaaa atggggatgc ctatgcatct gcaagtgata
      961 aagctgttta taatggacca ttcacattag aagattttga tggaccagga acagatacag
     1021 aatggactta taagaaaaat gatcattact gggacaaaga tacagtaaaa ctttctgaaa
     1081 tcaaagtcag tgtagtaaaa gaatcttcta ctgcattgaa cttattcaaa gatggccaag
     1141 cagatgacgt gatcttgtct ggtgagttag cacagcaaaa cgcaaacgat ccagcttata
     1201 cttctgttaa agaagcgcgg acaagctata tcgaattcaa tcaacgtgaa aagaattcac
     1261 cattcaacaa tgtgaaccta agaaaagcta tttcttattc tatcgatcgt gaagctttag
     1321 taaaacaagt actaggtgac ggatcagttg tctctacagg attgatccca tcagatatgg
     1381 taaaaagtcc aacaacgaac gaagactttg caaaagaagc tggaaaatta gtttcttatg
     1441 acaaagataa agcaaaagaa tattgggaaa aagcgaaaaa agaattgggc gttgattctt
     1501 tagaatttga tttgatggct tctgatgatg attcatcgaa aaaagtaatc gaatatgttc
     1561 aaaactcaat ccaagaaaac ttggatggcg taacagtcaa accaacacct gttccattct
     1621 ctgtacgtct tgaccgctca acttcgggtg attttgacgc agtactaggc ggatgggctg
     1681 ctgactatgc agacccaagc agctttactg atttgtttgt cacaggtaat tcatacaacc
     1741 gtggtcgctg gagcaatgca gaatatgacg aagcagttaa agcagcttca tcaacagacg
     1801 ctggcgatga acaagcccgt tgggatgatt tccaaaaagc aatcaagatc atctctgatg
     1861 atatgggtgt cgttccggtg taccaaaaag cagaaggtca cctagtgaat gaaaaaatca
     1921 aaggtatcgt tcaccatgcg gcaggcgctt catgggatta caaatggaca tacgttgaag
     1981 aataatcgtt aataccgtga gcatgagaaa aaacgaggct gcggcaaatg tcgcaacctc
     2041 gttctcatat tcacttatcc taacaaagaa aaaagcatga aaatagcaga aagagcaatt
     2101 agtattccgg caatgatcag ccaaaagctg tacataccgt agctggcaga cgagagcgag
     2161 gaaaattctg gttttttctt tcggttcctt gttcgtgcaa gatggaccga tcgctgaaaa
     2221 tgatcgaaga aacccgaaga aaagacccat aaaaaaccgc cgataatcag aaacggcaat
     2281 gaccataaga atagatcatt agataattga atgagtgtaa aggaatggtt gatgagattt
     2341 tttattaaaa taaccagaat cagtagaaaa gctactgcgt aggggataga tttttttttc
     2401 atttgattgc tcctttattt ttggttcatt atctaataat gtagcgaaac atctgtcaga
     2461 agtcaaaagc tagggggaaa cattagtgaa tagttatatc aaatatgtat taaaacgtgt
     2521 ctttttcatg atcatcacgc tttggttaat tgcaacaatc acctttttct tgatgcaatt
     2581 gctgccgggc acgccatata cgaatcagga acgtttgagt cctgaaacaa tcgagatgct
     2641 aaataaacaa gtcggtttgg ataaaccagt tatcgtacaa tatgggattt atctttctaa
     2701 tttgcttcaa ggagattttg gaatttcctt ccaatttaaa aaccagccag tcgcacattt
     2761 gttagctgga agaatcggac catccttgca gttagggtta caggcaatta tctttggaac
     2821 agtcctaggg acgattttag gaacgatctc tgccatgaag caaaatacat gggcagatac
     2881 atcttctaca ctcgtagcga tattaggtcg ttccattcca aactttgtat ttgctgtttt
     2941 attacaatat atctttgcaa tcaaattaca agttttgcca atcgcaaaat gggatggctt
     3001 tgtgtacaca atcttaccaa caattgcgtt ggccatgtcg ccgcttgctg actctgctcg
     3061 gtttatccgt acggaaatgg tggaagtact gcatagtgat tatgtggaat tagcaaaagc
     3121 aaaaggattg agccgttggg aaatcgcctt taaacatggt cttcgaaata gtttgattcc
     3181 attgatgact ttattaggac cgttagctgt agcattgatg accggatcac tggtggtaga
     3241 aaatatcttt gctattcctg gtatcgggga acaattcgtt aagtcaatca caacaaatga
     3301 ttatcctacg atcatggctg taacgatcct ttactcattt atgctgattt ttgttatttt
     3361 gatcgtggat ctattgtacg gtctagttga tccaagaatt cgtgtttcag aagggagccg
     3421 aggataaatg gaaatgatac caaagcgtta tgaaacagtt gcagatatcc ctgcggatga
     3481 atttcttcca ttacaaaaaa ataccgaaga agaacgtgaa cagattactg caccttcttt
     3541 gaattttatc caagattcat ggcgccgttt aaagaaaaac aaagctgccg ttatttcaat
     3601 ggttttacta ttggtcatca ttttgatctc tgtgatcaca atctttgttt ctcctcatga
     3661 tccaactgcc caaaacgtgg cttatatcaa tttgcctcct cgtattccgg gaatcaatat
     3721 cgatggattg aatggaaaag caatggttgg tggtgaactt gttgataaat atgctcaagc
     3781 aaacgtccct tcaaacgtaa atttcttttt aggaacagac ggtttgggac gtgatgtatt
     3841 aagccgctta tttatgggaa tgagaatctc gttgctgatc gcatttattg ctgcattact
     3901 agatgtaacg attggtgtag cttacgggtt ggtttctggt ttgttaggcg gacgtgtcga
     3961 taacgtgatg caacgttttt tggaaatctt atcaggaatt ccaaacctcg ttgtcatgat
     4021 tttgatgttg gtcgtttttg aaccaggaat tttttcaatc gtggctgcta tggccatcac
     4081 aaactggata ccaatggcac gtatcgttcg tgcacaaaca atgaaattaa aagatcaaga
     4141 atatgtttta gctggattga cattaggtga atcaagaatg aaaattgcat tcaaacatat
     4201 tttgccgaat atctctagtg tcattattat tcaaatgatg tttagtattc catctgctat
     4261 tttcttcgaa gcctttttaa gctttatcgg tttaggtctt acgcctccgt cagcttcatt
     4321 aggaacgatg ttgagcgaag ggtacaagac attcctgtat ctgccttatc tattatggat
     4381 tccggcagta actttatcgg ttatcatgat tggctttaac ttacttgctg atggattgcg
     4441 tgatgccttt gatcctaaaa tgaaagagtg aagacgatga cgaaaatttt agaagtgaaa
     4501 gacttagaaa tctcttttga tacgtatgct ggaaaggtac gagcgattcg cggtgtcaat
     4561 tttcatttga ataaaggaga aacattggcg atcgttgggg aatctggaag tggaaaatca
     4621 gtgacgacca gaagcatcat gcgcttgctg tcaagcaacg ccaatataga tgctggtcaa
     4681 atcctcttta aaggtaacga tatcgttgga aaatcagaaa gagagatgca aaaaatccgt
     4741 ggtaaagaaa tagcgatgat tttccaagat ccaatgacct ctttagatcc gacgatgccc
     4801 attgggaagc aagtcgctga gtcccttaga aagcataata aagtttcaaa aaaagaggga
     4861 caaaaagctg cattagaatt gcttaaatta gttggaatac ccgaagctga gaaacgtatc
     4921 aacagttatc cgcatcagtt ctcaggaggt cagcgtcaac ggatcgtgat cgcgattgca
     4981 ctggtatgtt atccggaaat cctgattgct gatgaaccta caacagcgct tgatgtgact
     5041 attcaagcac agattttgga gctgctgaaa gaaattcaga caaaaatcga tacatcaatt
     5101 atctttatca ctcatgacct tggtgtagta gcgaatgtcg ctgatcgtgt cgctgtcatg
     5161 tatggaggaa gaatcgttga agttggaaca tcggaagaaa ttttctacaa cccgcaacat
     5221 ccctatactt ggggactgct tggttcaatg ccgacattag atagtgcgaa cgatcgattg
     5281 tatgctattc ctggttctcc accggattta ttgaacccgc caaaaggaga cgcattctat
     5341 ccaagaaatg aattcgcgat gaaaatcgat gcagaacaag aaccaccatt ttttgaacta
     5401 tcaaaaacac acaaggcagc aacttggcta cttgctccgc aggcacccaa agtgacaccg
     5461 ccagaagaaa tccaacgtcg ctgggctatc tttaaagaga aacaaaaaca tacttacggt
     5521 caaggaggaa ttgctgatgc caaataaact acttgaagtt aaaggattga aacagtattt
     5581 caatgtagga aaaaagaatg aagtacatgc tgtaaatgac atcagctttc acatttatga
     5641 aggagaaaca ttcggtttag ttggagaatc tggaagcgga aaatccacta ctggccgtac
     5701 gatcattcgc ttgaatgaac ctactgatgg tgagatatta ttcgacggtc aagatataac
     5761 gaagatcaaa gataaaaaag gtttgacgaa attccggcat gatgttcaga tgatctttca
     5821 agatccatac gcttcgttga atcctcgtat gaaagtcaga gatatcattg ccgaaggaat
     5881 tgatgttaat ggactagcta aatcatctga agagcgtgca aaaaaagtcg atgatctatt
     5941 gagaactgtt ggattgaatc caagccacgg tactcgttat cctcatgaat tttcaggtgg
     6001 gcaacgccag cgtattggta ttgcacgagc attggcagta aaaccgcgtt ttatcatttg
     6061 tgatgaacca atttctgcac tagatgtgtc gatccaagcc caagtggtca atttgttgca
     6121 agatctccaa aaagaacatc aattgaccta tctatttatt gcacatgatt tgtcgatggt
     6181 taaacatatt agtgaccgaa tcggtgtaat gcataacggc ttgttattag agatgggaac
     6241 aagtgatgaa atctataatc acggtgttca tccttataca gaaagtttgt tatctgctat
     6301 tcctctacca gatccagacc atgagagaaa acgcagaaga atcaaatatc aacctgaacc
     6361 agatgacgga caagtacgtc aacttcgtga aatcgcacca gaacattttg tctatgcaac
     6421 tgagcaggaa gtcccatatt acgcaaaaaa actaaaacga caaaaggaat ctcttctggt
     6481 tgctaactag gagggacata gatgctaaaa gcttttaaat tccgtatgta tccgacagaa
     6541 gaacaaaaac agcaattgat tcgtactttt ggatgtgctc gatttaccta taaccatttg
     6601 ctgaaaaagc ggcagaaaag ttggcaacaa acaggagtag ccaatttttc gctcacaccc
     6661 gctacattaa aaaaagaata cccattctta aaagaagtag acagtcttgc tttagcaaat
     6721 gcacagctta atttggatcg ggcttttcgc aattatttca agggacgtgc cagcttccca
     6781 aagcttaaaa cgaaaaaaag tatgtggcaa tcttatacga ccaacaacca aacccgtacg
     6841 atctatctgg aaaatggcta cttaaagtta ccaaaacaaa aagaactaat caagataaat
     6901 cagcatcggc cagttgaagg atcgattcgc tccgctacga tttctgctcg atataatgaa
     6961 gaattttatg tcgcactact ttgtgatgtt tcgccagtaa aaaaagaatc tttagctaaa
     7021 tggatcggga tcgcctatca tccgaagaca ttgatccaaa catctcgacc tctagaagta
     7081 actttaccaa aatttcacca gacagaagaa aagctgcaac atgcacagcg gaaattgaat
     7141 gtgaaagtcc gctccgctca ccatagaaag atcaggttag atcaggcaag caattatcag
     7201 aaacaaaaga gaaaagtaat ggatctttat ctgaaacaaa agaaccagcg agaagattat
     7261 cttgaacaat tatctgggaa attggtcaaa caatatgact acttatttgt agaatctttt
     7321 ccaaaagaag aggcacatgc tgatttttct attcacgact ggcataaatt aataacaaaa
     7381 ttgcgttata agtcccaatg gtataataaa aaatttcttt tgatcaatac cgacggagca
     7441 gaagaaagca attccgtaag aaagagccaa gtagtagaaa aaatgggcag acattcggtt
     7501 atcaagggat aaaaaatgaa tgaaactgat aggagcgtta gggttagcct tgttaataaa
     7561 aagtcttaat gacttttgtt tctaggaatt tttcttttga agaaaagctc actaattatg
     7621 acaaaccaat aaaacaatag cagaaaaagt tcgtacgagc tttttctgct attgttaatt
     7681 ttttattcag aaagtttacg aatacatcaa aattctgaaa caaaaaacgt aactcacaaa
     7741 gagttgtttt tgcgtttttt cttgaatgat taggtctgaa tcttttgctc acaaccttat
     7801 tcatggtgcc ccggcttgag ctgatagatt tttgaaggac ggccgattcc tacaggacgt
     7861 tcaccgatct catcgaaata ttggagcata gctttcttaa aattggaatg atcgatcgtt
     7921 ttataatcga tccctaagaa tttagcaaat acttttcgag cttccgtaat cgtgaaatct
     7981 ttacctaaaa cttgtagaac ttgcggctcg tgttccattt tattgactac acggttgaat
     8041 gctttcaaga tgatttgact atggtcaaaa gccaaagtat ctttgccaga agactctcca
     8101 gtgactaaat caagcgagat agccacttct ccgctagata aatggatctt gttactatga
     8161 cgttctagtg tgaaccagcg tacttccttg gcatcatctc cggcgattag cggttcttct
     8221 ccaataaagg ctaagtagct gactgtgacg acccagcctc gcggatctcg attaggcgta
     8281 ctgaatgtat gcagttgctc aatattttct tttgaaataa ccacaccagt ttcttccttt
     8341 gtttctctta agacgctttc tccagttgac tcatcctttt ggacaaagcc accaggcaat
     8401 gcccatgaat ttcgataagg atgccctttt ctttggatca acaaaacttt cagctgatct
     8461 tcttctttgt tatagcacat cagtacgata tcgacagtca aagagggctt gtcatattct
     8521 ggacgttcct gtttttgata ccagttaaga aattcttctt cacttgcttt atgttcataa
     8581 tataattttt cttcttcttt agaagcaaaa cggttcatct gaccacctcc attgacagta
     8641 aaagtacttt ttaccttatt gtagcagact ctgaaaaaaa accaaggatt tacttgaaaa
     8701 atctaggtta tcatttaaat atgaaaaata aatggaagta tgagattgaa taaaaagaaa
     8761 agcagataaa acggcaatac tttataccgc tcttcacgaa tggcaaagaa gccacaaata
     8821 acgatatata cgtcaatgat aaaacgcaaa atccaagctg ctgtcataaa actgttcaaa
     8881 tgcccatgga ggttaaaata accatacata cgaatataaa aatagctgaa tattaaatgt
     8941 gctaatgaaa gaaaagcaat actaagtgta aaaaaaagat gtttcttctg catgattgtt
     9001 ccagtccctt actaagatag atagtcttta ttataccacg tgtgagggga gaaataaaaa
     9061 gccgtttttt agtagatatg aaacaaaaca aaaacccgca tttattcaac ctattcatcc
     9121 ttttaaagga tagattgaac gtagatagaa gtcgtttatc cagtcttttt cattttgaaa
     9181 aaaagctaac agcatgcttt ttttatggta tactagcaag gaaacgaatt cgcatcatgc
     9241 gataaaggag gaagagcccc gtggcagaaa aggaaacttt ttatatcact acgccaatct
     9301 attatccaag cggcaaactt catatcggca actcgtatac tacgattgca tgtgacgcaa
     9361 ttgctcgtta taaacggttg atgggctttg atgtgtttta tctcactgga gtggatgaac
     9421 atggtcaaaa aatcgaaaca aaagcagaag aactaggtgt aaaaccgcaa gaatatgtag
     9481 ataaaatggc ggctgatgtc aagaaattat ggaaaacgct tgatatcagt tatgataaat
     9541 tcattcgcac gacagatgat taccataaag cagctgttca aaaaatattt gatcgtttat
     9601 tagaacaggg agatatttac ttaggtgaat atgaaggctg gtactctgtt tctgacgaag
     9661 aattctttac agaaacacaa cttgcagaag tgtacaaaga tgacgaaggt aacgtgatcg
     9721 gtggtaaagc gccaagtgga cacgaagtag aattggtaaa agaagaatca tacttcttcc
     9781 gcatgagcaa atacgcagac cgtctattgg aatactatga agaacatccc gaattcatcc
     9841 agccagaatc tcgtaaaaac gaaatgatca acaactttat caaaccagga ttagaggatc
     9901 ttgcagtttc acgaacaact tttacttggg gagtaccagt caagagtgat ccgaaacatg
     9961 tggtgtatgt atggatcgat gcattatcca attatattac tgcactagga tatggctccg
    10021 aagatgaaag tctattcgaa aaatactggc ctgcagatgt tcagatggtc ggtaaagaaa
    10081 tcgttcgctt tcatacgatc tattggccaa tcatgctgat ggcccttgat ttgcctctac
    10141 ctaaaaaagt atttgggcat ggctggctat tgatgaaaga cggaaaaatg tctaaatcaa
    10201 aaggaaatgt cgtttatcct gaaatgcttg tggaacgtta tggtttagat gcactccgtt
    10261 attatttgct tcgtgctgtt ccgtttgggt cagatggtgt ctttacgcca gaagattttg
    10321 tttcccgtgt caattatgat ctggcaaatg atctagggaa cttattgaat cgaacgattg
    10381 ctatgatcaa caaatattgt gatggactcg ttcctgacta tgcctcactt gtcactccat
    10441 ttgatagtga actgtctacg acggctgcta acgttgtcgg acgttaccat gatgcaatgg
    10501 aaaaaatgga attcaataca gcattggctg aaatctgggt gctgatttct cgtgcaaaca
    10561 aatacattga tgaaacagaa ccatgggtat tagcaaaaga cgaagaaaag aaagctgaat
    10621 tgaatagtgt catgatccat ttagctgaat ctttgcgtat cgcagcgata ttacttcagc
    10681 caatcatgac agaaacgcca gagaagatct tcaatcaact aggattggac tctgaaacaa
    10741 tgaatcttga aggattacac tttggcgaat ttccttcagg aacaaaagta gttgcaaaag
    10801 gtacaccaat cttcccacgt ttagatatgg aagaagaagt tgcttttatt caagaaaaaa
    10861 tgtctgaagg tactcaaaca aacgaagata cagtcaaatg ggatccagaa gaaacagaac
    10921 ttgtttcaac gaaagaaaaa caaatcaaat ttgatgtatt tgaaaaagta gaactaaaag
    10981 tagcagaagt catcaactgt caaaaagtag aaggggcaga taagttgctg caattccgac
    11041 tagatgctgg agacagccaa gaccgtcaaa tcttgtcagg aatcgctgaa ttttatcctg
    11101 atccaagcga attgatcggg aaaaaagtcg tcattgtagc aaatctgaaa ccacgaaaga
    11161 tgcgtggaca aatcagtcaa ggaatgatcc tttctgcaga agcaccagat ggctctttac
    11221 aagtaatcga agcaccaaaa tccatgccaa atggctcaga aatcgcatag aaattcaaag
    11281 ggggtatgac actagttgtg tcataccccc tttttccgaa atttgagaag caattttcgt
    11341 aaattccttc ttattacttg aagccaatca tataatacag aaacagagaa ctatccaaaa
    11401 taaatcctag aaaaagaagt acgagcttcg ctgatttttg ttaatttaga ataatactaa
    11461 taggaatcgt aaaaacagaa aaaaagcata atgataaagg gtcgggcaga ttttaattga
    11521 ttcatctggt actctttttt attgtcctta cattcataaa gtttgaatgc aatgacttta
    11581 ttacatctat atttatatct aacagtagcg ttgatgtgtt aattataagt tctatcactt
    11641 tatgttcaat tagtttgtgc tcttatatgt gtgattgaaa atatagataa aaaaacaatc
    11701 atcggctttg acttcgatga ttgttttctt tattttgtaa aaatcctatc acttgatttt
    11761 atggtaacct tttatttttg tcttcaaacg gcttggtaag agagcagtaa gcagtgcaaa
    11821 tgggatcgaa aagacatacc agaaaatcgt acgtccgata ccataatgtt gatagagctt
    11881 tgtcaacatc acgtgtaaca agatcaaccc taaaagcaag gagccagcta taaaccaatg
    11941 attatcccaa tgcatgactt cactaagtgc catcactcca aaaggataga aataaaaata
    12001 gatcccgact aaggtcatcc gccagttctt tgtaaatgtt ccgatattca ataaaaagaa
    12061 aatcgctgaa agcaaaatac cgataggcgg aaaaagccca accaaaaagg aatacaacat
    12121 cccccacaag atcatcacag gacctttgat caatgcaacg attgcgatga acaaagtcag
    12181 caatattagt ttttgtgata acgttgcaac agtcattaat ccaaataaaa ttaatgtgaa
    12241 ccaatgccaa tgggactgct gttgttcttt acgggaaaaa tcgacccggt tggataaata
    12301 atctgctaat ttcggataag aacgcttaaa atccataaac tgttcctcct tcgtggaaaa
    12361 ggtaggttta gtatacgaaa aataaaaaag aaatacatcc gtcttaagag ggagtttttc
    12421 actgaaagtg gagaaaaaag aatggtaaat taaaatattc tcagttatac tgaaaattat
    12481 accaaatttc aaagaagaaa gaggatggga atgaaaggca aactaaaacg agaaatcaat
    12541 ctgtttggcg cattagctac cgttatggga accgtgatcg gtgcaggcgt cttcttcaaa
    12601 actgctgctg ttactgctag tactcagtct acaagtttga cattgctagc ttggcttttg
    12661 ggtggtttct taacgatctg tgctggattg acagtggctg aattagctac agcaatcccg
    12721 gaaactggtg gagctgtaaa atatattgaa gctgcgtatg gaaaattgcc cagcttttta
    12781 ttaggatggg cgcaaagctt gatttacttt cctgctaata ttgctgcatt atcgattatc
    12841 tttgcgacac aattgacgaa cttattacaa ttatctaccg attatctttt gttgattgca
    12901 gtgatcaccg ctgtttctgt gacagggttg aatttgttag ggacaaaagt cggtgcttct
    12961 gtacaatcag tgacgttgat tgttaaatta ataccaattg ctgtgattgt catatgggga
    13021 ttattgaccc caggacaagg aactgtccag ttatttccaa tcgaagctgg aaaagatgtt
    13081 acttttgtcg aaggattgag cagtgcgttg ttagcaacgt tgtttgctta tgacggctgg
    13141 ttaggtgtcg gtgcaatggc aggggaaatg aaacggccgg aaaaagattt gcctaaagca
    13201 attattcttg gtttgagttt tgtaacagtc gtttatctat tgatcaattt tgtttttttg
    13261 aaaactttgc cgattgatca tcttgccggt aatttgaatg cagcttctga agcatcagac
    13321 gtcatttttg gaggaatcgg tggaaagctg gtcactattg ggatcttgat ctcggtatac
    13381 ggtgcattga acggttatac actaactggg atccgcgtgc cttatgcgat ggctttagaa
    13441 gacgagctac ctttcagtaa acagctgaca aagctttcaa agaagtttac ggttccttat
    13501 gttcctgcgg ttttccagtt agcagtagct tgtattatga tgagccttgg ttctttcgac
    13561 tttttaacag acatgttgat ttttgtgatg tggctattca gtttactgat ttttatcggt
    13621 gtctttattt taagaaaaaa agcgccggaa ttgccacgtc catataaagt accactttat
    13681 ccaatcgttc cgattattgc gattctcggt gctattttca ttttaggtat gacgatgatg
    13741 acgcaaacga aattagcatt gatcggtata ggtgttacct tgataggtat cccagtctat
    13801 tatcaaaaaa agaaaaaacg ctcagaagag taaaatattt caacaacaga cagaaaaagt
    13861 acgaactagg tcctctagat cttcgtgctt tttctatttc aataaaacaa gttcttctat
    13921 tttgattttt gctacttctg caagtagtgc ttatgtgcta aagtaagaat gaaaaggaga
    13981 gataaacatg atatttgatt ctcataccca tttaaatgct gaacagttca atgaagatat
    14041 tccagaaacg attcagcgag cacaagaaat gggggtaacg aaaatggcgg tcgtgggctt
    14101 cgatacgcca acgattgaaa agtctttgca attaagccat gactatccaa atatatacag
    14161 tattatcggc tggcatccga cagaggctgg cagttatacg aaagaaatcg aaaataaatt
    14221 acaagaacaa ttgactttac caaaggttgt agcattaggg gaaattggct tggattatca
    14281 ttggatggaa gacccgaaag aagtccaaga taaagtattt cgcagacaga ttgctatcgc
    14341 aaaagaaatg aacctaccaa tcagtattca tactagagaa gcacttgaag acacgtatca
    14401 aatcctaaaa gaagaagatg ttcgagatat cggtggaatc atgcatagtt tcagtggtga
    14461 ttatgaatgg gcaaaacgat ttcttgactt agggatgcat atttcattta gcggtgtcgt
    14521 cactttcaaa aaagcattag acgtccaaga ggctgcagca aatgtaccaa tcgaacgatt
    14581 acttgtggaa acagatgccc cttatctggc accagttcct tttagaggaa agcgaaatga
    14641 accaggctat actcgttata cagtagaaaa gattgctgaa cttcggaatc ttccgacaga
    14701 agaagtggct gcccaaacat ggacaaatgc gcaccgatta tttggtctaa gcgaacatgg
    14761 ataagatgaa gatccaagag attatagtcg ttgaaggaaa agacgataca agaaggctgc
    14821 gggaagtagt tgatgcagat acgatagaaa caatagggtc tgcaatcaat gaagagatcc
    14881 tgatgcaaat tgaacatgcc caagagactc gtggagtgat cgtttttacc gatcctgatt
    14941 tttcaggaga aaaaataaga aaaatcatta tggaagctgt tcctcaggca aaacatgctt
    15001 ttttgccgcg cacacaagct aaaccaaaga aaaagggaag ccttggtgtc gagcatgcaa
    15061 gtgatgctgc tattttagaa gcattgaaaa aggtcgtgac gccagacatt tcccaaacgg
    15121 ctgtttctga aatcactaga caagaccttt tagactatgg tttaatagct ggggcacaag
    15181 caaaagaaaa acgagaaaaa ctaggtgacg agttgcgcat cggttacacg aacggcaaac
    15241 aattggaaaa acgtttgaag atgtttcgta ttacgccgaa agaactcgcc caagcaatga
    15301 aaatagtgga ggaacaacat gagtgaatat cgtgaaattg cgacaccctc tagaacaaaa
    15361 gagattttat tgaaacatgg tttttcattt aaaaaaagtt taggacaaaa ttttctgact
    15421 gaaccaaata ttttaaggaa aatcgtagaa actgcgcaaa tagatgaaca tacgaacgtg
    15481 atcgaagttg gaccaggaat cggtgcattg acggaacaat tagccaagca tgcaaaacaa
    15541 gtcgtagcat ttgagatcga tgatcgtttg atccctgtat tggctgatac gatgcaaccc
    15601 tatgacaatg tgactattgt acatcaagat attttaaaga ccgatttgtc aacagctgtt
    15661 agagaaacat ttcatgagga gcttccgtta aaagtcgtag cgaatttacc ttactatatt
    15721 acgacaccga tcatgatgca ttttcttgaa tcagatttgg ttgtggatga actagtcgtc
    15781 atgatgcaaa aagaagtagc tgatcgtatc tctgctgaac caggaacgaa agcttatggt
    15841 tcattatcga ttgctgttca atattatatg gaagccagtt tagcattcat cgtaccaaaa
    15901 acagtatttg tccctcagcc aaatgtagat tcagcgattt tgaaactgac aagaagagat
    15961 ataccggcag tcgaagtgac agacgaaaaa gcatttttcc gattgacaaa agcggcattt
    16021 cagcaaagaa gaaaaacgct ttggaataac ttgcagcatt cgtatggcaa agatgaccag
    16081 acaaaagcat ggttagcaaa aagcttggaa acagctggaa tcgatccaaa acgtcgtggg
    16141 gagactttgt cattacaaga attcgccgca ttaagcaatg cgatgtcaga aaatatgcag
    16201 taaaaaaaga ggttgtgaca gaagttgtga aaaagctgtc ctgcatcaag taataagaac
    16261 agccaaacaa aaatagttcc tcatgttttt cgtttgtttg ggcttattac cgcagatgca
    16321 agcttttgaa caccgtttaa gaaagaggtt gtgggcctgc cgtttaactc taagtcacaa
    16381 ggaacaattt caaaaaacag cttctcatgt ttttgagtta gttggtctta ttacttgatg
    16441 caagcttttg accactatta tgccgaacaa ggagatgtga cacaaatttt gtcacatctc
    16501 ctatttttgt ttcttgatca aaaaattctt atacttgata ctcacactca ttttgtatgg
    16561 atttagaaaa gaatatgtgt cttgacagtt attagtaagt gagttatact aagtcgacat
    16621 ttatgtgtca agacatatta ggagagagaa aaaatgagaa aaaattcagt tcgaaaacta
    16681 acgatttctg ctttattgat tgggatgggg gtcattatcc caatggtcat gcctaaaatc
    16741 atgattccac cagcttcctt tactttggcg tctcatgtgc cattatttat tgcgatgttt
    16801 tttacaccag gagttgcagt ggctgttgcg ctaggtacga catttggttt ctttttaaca
    16861 acgccggtta tcatcgcatt gcgagcattg tcacatcttg tgtttgcgtt gattggggct
    16921 tggtacttgc aaaagcatcc gagtatcgtt ttaaaaaatg gacagtttac ttttgctaat
    16981 tggcgtttcc aagggttcaa ttttgtgatc ggtgtgattc attctttagc agaaatgcta
    17041 gtagtcagca tcttttactt tattggtaat atgcctgaaa catattattc tcaaggatat
    17101 ttttatacag tattcatctt gatggggatt ggtgggctta tccacagttt agttgattat
    17161 aatattgctt attttatagc aggaacactc agcaaatatt ttgatatccc cgtatttacg
    17221 gcagcaaaaa aagaaaatcg gaaagagtcg attcaagggt acgcgaagaa aacgacatat
    17281 tagacatgat ctctcaaaaa aaaaactctc atctagtaat ggataataaa tcctctaaga
    17341 tgagagtttt ttgtcctaaa aagagctgca ccaacatctt ctggcacagc tctaacaggg
    17401 ggcaatttat ggaagtttga tgacttgttc tgtttcttta tcaaagaagt ggcctttgct
    17461 gatgttgaag gcaaggtcga ctatttcgcc tggtttatgg aaatctcttg catctacttt
    17521 tgagatgaac tcagtagaac cagtttttgt gtaaagcatc gtttcagctc caagtaattc
    17581 ggaaacgaca acttctgatt tcactacggc ttcaggtgaa gcttcaaggg ctacttgttc
    17641 actgtggatg tcttctggac gaataccgaa gataacttct tttccttcat aacctttttc
    17701 aactaatact ttgttttttc cttcaggaat acgaagctta agcccatgtc catctgtgat
    17761 aacaccattt ttcaatgtca cattaaagaa gttcatggca ggagatccaa taaagccggc
    17821 aacgaacata ttgacgggtg tatcataaac ttctttcggt gtaccgattt gttggataaa
    17881 tccgtctttc atgataacga ttcggtcagc catagtcatt gcttctgtct gatcgtgagt
    17941 aacatagatg gtcgttgttt caagacgctg gtgaagtttg gcaatctcag cacgcatggc
    18001 tacacgtaat ttagcatcta aatttgataa tggctcatcc attaagaaga cttttgcatc
    18061 ccgaacgatt gcccgtccca atgccacacg ttggcgttgt ccaccagata aagcagctgg
    18121 ttttcgtttc aagtattctg tcagacctaa gatctctgct gcattgtcca cacgtttttt
    18181 gatgtctgct ttatcatatt tacgtaattt caagccaaaa gccatattat caaaaacagt
    18241 catatgagga tacaatgcgt agttttggaa aaccatggca atatcacggt ctttaggtgc
    18301 cacgtcattc atgacttttc cgccgatatt tagttctcct tcagagatat cttccagtcc
    18361 cgcgatcatt cgcaaagtag ttgactttcc acaaccggaa ggaccaacaa aaacgataaa
    18421 ttcacggtct ttgattgata gattaaaatc agttacagaa taattttctg cattgtcata
    18481 ttttttatat acgtttttca gagccatttc taccataata cgttccacac tttctttttt
    18541 agttttatta actatttaaa gtataaatga aagcgttaac taacttcaat gtcatgttga
    18601 acaagaattt tttctttttt ttgtcaatgt gctagtttta agctttaata aatatccata
    18661 agaaataaaa actgatacac tggagtaaat aagtatgtta acaatatcaa aaggagtgtc
    18721 tcttatgaaa atcgctttga tcgctcatga ccgaaaaaaa cctatgatgg tgaaattagc
    18781 aacagcgtat aaaacaatat tggaaaaaca tgaacttttt gctaccggga cgactgggca
    18841 gaggatcacg gaagcaaccg gcttgccgat ccatcggttt aaatccggac cgttaggcgg
    18901 ggatcagcaa attggagcca tgatttcgga ggatcagtta gatttggtga tttttcttcg
    18961 agacccgctg gctgcacagc cccatgaacc agatgtaaca gcacttatcc gtttatctga
    19021 tgtctatgag attccgttag ctacgaatat cggaacagct gaggtgctac taagaggact
    19081 ggaagccggc ttcctagatt ttcgaagaat cgtccatgaa atagacaagc atccactttc
    19141 attttgatgg ataagcgtat ggaaaaataa cgtttaatgt tcgcctatat gtagctataa
    19201 aagctaaaca aactgataat atgcgatata atacgaacgt attggataat ataaacaata
    19261 atattagttt ttcgtatttg cagtaaatgt gaaaatatgc tatagtttct gtgggaaaag
    19321 atcccaaaaa taatacagag tagaaaataa agcgttaagt gctaagagga tgggatgttg
    19381 cctcttggac gaaggacgta atgttcgcgg ttttattttc gcattcgctg catgatacga
    19441 tgtagcagct cttaaaggag atgctaaaaa tgaagaaaca ccggttagac gcacgtatga
    19501 ttgctattat gggcttattg attgcattga tggtcacttt gtcccgactg gtagcaatcg
    19561 agaccccatt tattaaaatc agtgtcacat ttattccaca agtgatcatg ggcattcttt
    19621 ttggtccttt ttggtcaggg ataggcgcag ttttagctga tttagtagga atggccctct
    19681 tctcgaaatc tgcattcttt attggattta cattgaatgc atttattgaa ggagcaatct
    19741 acggattctt tttctatcga aaagagatca cttggaaaaa tgccattctg gctacgctta
    19801 gtgtcacgct gatcatcaac ttatttttga caccattatg gcttgcgttg atgtaccatg
    19861 tgccattgtt cagctgggtc gtttgggcgc cccgcctgtt aaaaacagta atatggttac
    19921 cgattcaatc aattgccatt tattacgtag gacgtagtat tccttacaaa aaaattttaa
    19981 gaagtttagc gatccacgct aaataaaaaa caagcagtct tcccatccca gaagactgct
    20041 tgtttttatt gtgctgtgaa ttgtgcttga tagagccgtc ggtaataacc atctttttga
    20101 agcagctcat catgggtgcc catttcgaca atagctccct gatccatcac taatatctgg
    20161 tccgcatttt tgattgtgga caggcgatgg gcaatcacaa aactggtttt tccttccatc
    20221 attgctaaga aggcttgttg gatttttttc tctgtcaatg tatcgactga gctggtagct
    20281 tcatctaaga tcaacatagg gggattactg atcatcgttc gagcaatcgt caacagttgg
    20341 cgttgtccat ctgatatctt taacccttga ccgccaatct tagtatctaa tttttccggc
    20401 aaacgttcta caaattcata catataagag gcttttaggg catcattgat ttctgcgtca
    20461 cttgcttccg gattcccaaa acgaagattt tctcgcaatg tactgtcaaa tagccaagta
    20521 tcttgtaaca ccattccaaa acttttacgt aatgtatcac gttggataga gcggatgtca
    20581 tggtcatcga ctgtaattgt tccttttgtt gtgtcataaa aacgcatcag caaattaaca
    20641 agtgtggatt ttccggcccc tgtttttcct actatagcaa tggtttgacc tggataagca
    20701 gtaaaattga aatcttcgat caaacgctgc ttggggtcat aagaaaaaga aacatgatca
    20761 aaagagactt ttcctttgat atttttgggt tccaaggcat aggcatctgg caaatcaggc
    20821 ttttcttctg gctgatcgat caaatcaaat gctcgttcta gaccagaaaa agcggtttgc
    20881 agttgtgtcg tgattccaga aagttcgata aatggtttag aaaactgact tgagtagatc
    20941 gtaaagctgg aaatcacacc aacagtgatt gacgaaccac tcttcaatgc caataggccg
    21001 ccaactaaac caatcgataa ataagcaagg tgatctacaa aacgagaaga cgggtttgtc
    21061 aaggaagaag aaaattgtgc ccgttgtcct ttttcatata gatcagcatt tagcagttca
    21121 aattgctgtt gactagcttc ttcttgctga aaagctttca cgattttctg gttaccgatt
    21181 cgttcggtca caaagcccga gatatcccca ataatccttt gctgtctatc gaaatttgtt
    21241 tgtgaagcac gagcaacgat ccaactcaca aggaaaataa ttggtgtaga acaaagaacg
    21301 accaatgtca gaagtggact tagacgcatc ataaaaataa acgaaagaat gatgattgcc
    21361 actccagaaa ataattgatt gaaggccgca gaaattgcaa tggaaatatt gtccatatca
    21421 tttgtaaatc gactgacgat atttccgtgg gagttttgat cataataacg aagtggcaat
    21481 cgattcagat gagcaaatgc atcttttctt aattttgcaa cggataagta agctaaccga
    21541 ttgcttaaca cttggatgag ccattgactg actacgttga ttaaaacgat accaccgaat
    21601 agaataagta aatggaacag tcgattgaag cggacctgtc cttctccaat catttgatcg
    21661 atactttgac caatttgtaa cgtcatcaca actgttgcaa tcccattgat gattcctagt
    21721 atcacagcta gccaaatgtt ttttgcatag gcagtaaggt aaggaatgaa tcggcggagc
    21781 gtctgcaagg ataatttttt tactttcata actattcttc ctcctgtgat gcgacgattt
    21841 cctgatattc tgctgattga gcaagtaatt cttcatgggt accctgagca gcaatcttac
    21901 cttgatccat cactaagata tgatgtgctt cttgaataga gcggacacgt tgggagataa
    21961 taatcactgt actgccaaga taagtctgta gcgccttacg caaatccaag tctgtttgat
    22021 aatccaacgc acttaaagag tcatctaaaa tcagtaatgc tggcttggca atcaaagctc
    22081 ttgcaatcgt cagtcgctgt ttttgtccgc cagagaaatt tttcccattt tccatgactt
    22141 ttgtatctaa accatttgga agctgacgga caaattcttc tgcctgagcg attttcagag
    22201 cttcccaaca ttctttttct gtggcgtctt tcttacccca ttgaaggttt tcacgaatag
    22261 tcccggtaaa tagtacagaa gtttggggaa caagagcaat ttgctgacgg agatgatcca
    22321 gctgccaaga acgaacatcc attccgtcca ttataactgt accggaactg acatcataga
    22381 aacgcggaat caattgtgtc aaagtcgttt tcccgcttcc ggttggacca atgattccaa
    22441 ggactgtatt tcgcttcagc tgaaatgtaa tattttggag agaaagacca gaatcaggag
    22501 tataacggaa gtcaacatgg tcaaaagcga taattgcttc agaagaaaaa tcgggagtag
    22561 tactgacctc agcttcagga atcgaatttt ctgtgtctag cacctctttc acccgatttc
    22621 ctgaagcttc ggcacgtgtg aagatcacga cgaggtttga aacaacgatc agtgccaaaa
    22681 gcatttgatt catataattg atcaaagcaa gaacttgtcc ttgttgcaag ctaccaatat
    22741 ttactttgat cccgccaact gtcagcaaac agatgatccc gacattcatg atcaaggttg
    22801 tagcaggtga taatagggca gagatattgg aaacacgtaa ataattgttc gctagatcat
    22861 ttgtgctttt atcgaacttc tctgtttctt tttttgtctg cgcaaatgcg cggatgacac
    22921 gaacaccgct aagattctga ctaacgtgtt cattgagctg atctagtttt tcttgcactt
    22981 tttggtagag aggaacggtt tttttgatga taaaaaataa aatgattgag aaaataggca
    23041 ataatgctaa gaaaaacaga cctacttccc agtcgatcac aaaagccatg accactgacc
    23101 cgatacttaa aaacggtgct ctgatcaata aacgaatgac cattgctaaa gctagctgaa
    23161 gctgattgat gtcgtttgtc attcgggtga tcaaggtatc tgttccaaaa tgattcaact
    23221 cgttgtgaga gaaagtattg atttttttga ttagctgatt acgtaactct gtaccaaatc
    23281 cttgtgaagc tactgaagca tagtattggc aaatgatagc acaaatcaga ccaatcacag
    23341 acatagcgac catccataag cccatttcaa tgatttttcc tcggtcattc atttttagtc
    23401 cattatcgat caaggaagcc atcattaaag gcaggacaag ttcgaagaca gcttcaagaa
    23461 atttgaagaa cgggcccaaa atgatttgct tacgatagtc ttttgcataa cgtaataact
    23521 gaatcatatg attcctccta ttatttattt gtccacaatt gaaaaaacaa gtactaccac
    23581 attgtagctt aggaaattta gaatgaaaag agaacaagcc ttttcataaa tgaaagtttt
    23641 attatgaaga agctttagaa attgaataga ctattttttt taaaaaaaag aaaatgagaa
    23701 aacatgccag tctcttcatt cataggaacg tctattttac ttaatttttc tataaatgaa
    23761 ttctgcataa aaaatatatc aagtaaatgt ttatagatga aactctattt acttcttttt
    23821 taatttgttc ttgcaatctt ttttgctacg taatttttgt aaaaaagtgg gttgagagaa
    23881 atctaattga ttttcaaaga aggagtatga aaatgagaaa aatatttatt cgtttattgt
    23941 gggtactatc ttagaggaat aaataatagt ggagaaaact atggtttggc gcagattcta
    24001 tcaggtttga tggaacgaag acaacctccc gacatcaagt caaaagctac ttgagatttt
    24061 gatctgaaaa taaaaaaaga aaacaaggta ggaacatcaa tgtttctacc ttgttttcgt
    24121 aatcaatggg aaaaaactaa gagtggtgta cgtaatcaat aaaaaggatg gtgacaacag
    24181 tacctcttta ctctctgaaa gcatcataac tcgtttttcc tcattatgcc aataaattgt
    24241 tgttatacca catataagga tttgtaatag ctaaaaacag acaggaaagt tgaaatctgt
    24301 gaatcttatt ttgtatggaa ggatttatag aacgcaaaaa tcaagataaa gatgaataaa
    24361 aatcctgttt cggctatata ttgcgtatct acgccaataa aacgagtttg ttctgtcata
    24421 gccatataaa gcaaaggcaa aaatacaact acagcgagat aagatccgat gatttctcga
    24481 aaagttttcc aggaagtatg tttcctgttt ttatatttat ccgatttcat tgaatagtca
    24541 tagcctcctt attctttcat atgttgaaat tccagtataa aaactggaag gaactgtacg
    24601 ttttttagaa taaaaattat aagggataag taggctgctg tctataataa gatatatttt
    24661 tcaattcttt attaaagatt ttattgacaa aatgaatccg ttttctatga tggaggaggg
    24721 gtgaataagt tgttgaagaa ggaaatttta tgttacttag agaaccagac agcctttttc
    24781 gatttagaac atatgaacga ggtctttaca gcacaaaggt tagctgatca ttttggggta
    24841 aaaagaaaca cagtcagtca ttatttgaac cagttaaatg atgagcaact actagtcaaa
    24901 atcaattctc gaccagtgat ttatttccac aaagaagctt ttgaaaaaca attttttaag
    24961 ttacgcacga atttttattt ttctatcgat gatctcaaag aggaacagcc ttttttcgcg
    25021 caaccaaaag acttattctc ttttatgatc ggatacgacg ccagtttgaa agaaagtatc
    25081 gaacaaatca aaacagctct ttactatcca gatggcggat taccactttt gattacaggc
    25141 gaaagtggca caggtaaaag ttatttagtt aatttggtct atcagtattg tctgcttcat
    25201 gatctgctag aagattctgc accattcatc acttttaatt gtgcacaata tgcagataat
    25261 cctgaacttt tgactagcaa tttgtttgga catgttaagg gagcgtacac tggtgcagaa
    25321 gaaaataaaa aaggggcttt tgaagcagca gatggtggga tcttgttttt agacgaggta
    25381 catcgcttaa caccagaagg tcaggaaaaa ctgttcacct atcttgatca aggtgtgatt
    25441 tatcgtatgg gagaacctaa tttgtcacgc cggatcaaaa cacgtctctt ttttgctacg
    25501 actgaagaga ttactagtca tttcttaaca actttcattc gaagaatacc tatccgaatc
    25561 gaactacctg cacttaatca gcgtagtcgt aatgaacggc tagaactgat ttactctttt
    25621 tttctcgaag aacaaagaaa aatggggagc cctttgtcag tcagtggacc agctttgtca
    25681 cttttaacaa gtcagaaatt atccggaaac ataggagagc tgaaaaatat cgtcaaagtt
    25741 tcagcagcaa aagcatatgc tgaacagcgt gaacagcctg aaatccatgt cacgatccat
    25801 catttgcaaa aagaactact tgcgcagcct attactaaaa atacagcaca gcaaggaatc
    25861 tatattacgc aagaccagac cttagagcaa ttaatggaga agcaacagcc cgaacagcag
    25921 cgaatcgtta aaagttttga gcaaatccta attgatttca agaaaaataa ctgtctgcta
    25981 tattcttgcg aaggaaaatt aaagcaagaa gtgattcaat tatttgattt tttgctgttt
    26041 gagaccagta gacaagaaaa acacgagctg ctggtttatt ggacacaatc tatccgagag
    26101 acctttcgac aaatggaaac tgcctatcaa atcaaattcg atggaaacag tgtctatgcc
    26161 ttgagttact atctctatca gcgaagcact atcaaatggt taccggaaga acatgatatt
    26221 atccaactaa tcaaagaatt agaacaacaa gtttctcagt cttatccatc tagttatcac
    26281 tatgtccaac gaattctgga attaagtaag atcaaattag atattgaaga cacaagtatg
    26341 gatcggattt tactgacgat ctatctaaaa aaagcgaaat ggtcaaaaga aaaacgactg
    26401 ccaaaagcga tgattgctgc tcacggttat gcaactgcta gcagtatggc aaatgtagta
    26461 aatcgtttat tacaagagga tctatttgaa tcatttgata tgccactaga tgtcacacca
    26521 cagcagattg cttcagaaat ccttgattat tgcgaaaaca gcgatgtttc aaatggattg
    26581 atcatattag tagacatggg atctctgaaa gaaatccatc agttttttaa aaaacagtta
    26641 tccgtaccgc ttttgatttt gaataatgtg acgacacctt tagcaatcac agtaggggaa
    26701 tgcctgcaaa aaaatgtatc attagaagag atagcagaag agactgttcg acagatccag
    26761 ccagaatggc gactgctcta tccagaggaa aacaaaccca aagcactcat tactacttgc
    26821 ttcactggaa tcggtacggc ggtccactta agtgaactgc tagaaaaaag cttacctacg
    26881 gcttgtcagt tgaaaatcat cccatatgaa tatcagcaat taaaagataa gaaaaacagt
    26941 gacccattat tttccatata cgaagtcgta ggaatgattg gcaccacaga tcctgatatc
    27001 accaatatcg actatctttc attagaagac ctcatctcgg gggaaaagat gtctgtacta
    27061 gctgaatggt tggacactac tatgaatgct tcagagaagg aaatattcaa tcagcgcatc
    27121 attcgtaatt tttcattgga aaaagtactg gattccgtga ctattctaga tacagagaag
    27181 gtgatgggag aaatcgatca ttttatgaga gaattagagg tgcagttgaa tcgaagcatc
    27241 agcaatccca aaaaattagc gttgtatgtc catgtgagct gtctcattga acgattgatc
    27301 cgacaaatgc caatcgaaac ctatcaagga ttagagaaat ggaagcagtg tcaaaaagaa
    27361 gggttatctg ctattaaatc tgcgtttagt gtcattgaag agcattatag tgtcatgatc
    27421 cccaattctg agattgccta tatctatgat attttgagtg aagatacaga gtttctctcg
    27481 atagaagaag aattttgaac ataagcaagt tgttgccact aattttggca tgcaacttgc
    27541 ttttttagta tgtgtaagtg aaaagaggag atgaaaaagt ggatagagaa attatcttag
    27601 cttcccatgg aaaatttgct tcagggatat tagattcatt agagctgatc tatgggaaaa
    27661 atcattctat tactgtattc gattgctata ctgatgagaa ttttgattta gcggaaacag
    27721 taaggcagct ttttatgagg tacaaagata aagaaatcat t
//
LOCUS       DACCKU010000032        27713 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.034, whole genome shotgun sequence.
ACCESSION   DACCKU010000032 DACCKU010000000
VERSION     DACCKU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.034"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(45..1511)
                     /locus_tag="IVN13_001284"
     CDS             complement(45..1511)
                     /locus_tag="IVN13_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP7011782.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="IVN13_001285"
     CDS             1898..2494
                     /locus_tag="IVN13_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7011783.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="IVN13_001286"
     CDS             complement(2552..3034)
                     /locus_tag="IVN13_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011784.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="IVN13_001287"
     CDS             complement(3140..3337)
                     /locus_tag="IVN13_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011785.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="IVN13_001288"
     CDS             3632..4390
                     /locus_tag="IVN13_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP7011786.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="IVN13_001289"
     CDS             4450..4878
                     /locus_tag="IVN13_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011787.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="IVN13_001290"
     tRNA            4924..4996
                     /locus_tag="IVN13_001290"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="IVN13_001291"
     CDS             complement(5146..5517)
                     /locus_tag="IVN13_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7011788.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="IVN13_001292"
     CDS             5732..5917
                     /locus_tag="IVN13_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011789.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="IVN13_001293"
     CDS             complement(6000..6188)
                     /locus_tag="IVN13_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011790.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="IVN13_001294"
     CDS             complement(6212..6475)
                     /locus_tag="IVN13_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011791.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="IVN13_001295"
     CDS             6633..6890
                     /locus_tag="IVN13_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP7011792.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="IVN13_001296"
     CDS             complement(6961..7152)
                     /locus_tag="IVN13_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011793.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="IVN13_001297"
     CDS             complement(7293..7613)
                     /locus_tag="IVN13_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011794.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="IVN13_001298"
     CDS             complement(7636..8070)
                     /locus_tag="IVN13_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7011795.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..9043
                     /locus_tag="IVN13_001299"
     CDS             8405..9043
                     /locus_tag="IVN13_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7011796.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(9366..9449)
                     /locus_tag="IVN13_001300"
     tRNA            complement(9366..9449)
                     /locus_tag="IVN13_001300"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9413..9415),aa:Leu,seq:cag)
     gene            9977..10477
                     /locus_tag="IVN13_001301"
     CDS             9977..10477
                     /locus_tag="IVN13_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7011797.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(10667..11011)
                     /locus_tag="IVN13_001302"
     CDS             complement(10667..11011)
                     /locus_tag="IVN13_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP7011798.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(11165..12085)
                     /locus_tag="IVN13_001303"
     CDS             complement(11165..12085)
                     /locus_tag="IVN13_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7011799.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            12272..12763
                     /locus_tag="IVN13_001304"
     CDS             12272..12763
                     /locus_tag="IVN13_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP7011800.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(12787..15162)
                     /locus_tag="IVN13_001305"
     CDS             complement(12787..15162)
                     /locus_tag="IVN13_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP7011801.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            15488..16114
                     /locus_tag="IVN13_001306"
     CDS             15488..16114
                     /locus_tag="IVN13_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7011802.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            16137..16841
                     /locus_tag="IVN13_001307"
     CDS             16137..16841
                     /locus_tag="IVN13_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7011803.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            17033..17866
                     /locus_tag="IVN13_001308"
     CDS             17033..17866
                     /locus_tag="IVN13_001308"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP7011804.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            18063..19991
                     /locus_tag="IVN13_001309"
     CDS             18063..19991
                     /locus_tag="IVN13_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7011805.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            20129..20584
                     /locus_tag="IVN13_001310"
     CDS             20129..20584
                     /locus_tag="IVN13_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP7011806.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            21054..21323
                     /locus_tag="IVN13_001311"
     CDS             21054..21323
                     /locus_tag="IVN13_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011807.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            21723..21977
                     /locus_tag="IVN13_001312"
     CDS             21723..21977
                     /locus_tag="IVN13_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP7011808.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(22066..23112)
                     /gene="holA"
                     /locus_tag="IVN13_001313"
     CDS             complement(22066..23112)
                     /gene="holA"
                     /locus_tag="IVN13_001313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP7011809.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(23150..25423)
                     /locus_tag="IVN13_001314"
     CDS             complement(23150..25423)
                     /locus_tag="IVN13_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP7011810.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(25416..25955)
                     /locus_tag="IVN13_001315"
     CDS             complement(25416..25955)
                     /locus_tag="IVN13_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP7011811.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(26091..26309)
                     /locus_tag="IVN13_001316"
     CDS             complement(26091..26309)
                     /locus_tag="IVN13_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP7011812.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(26306..26551)
                     /locus_tag="IVN13_001317"
     CDS             complement(26306..26551)
                     /locus_tag="IVN13_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7011813.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(26667..27395)
                     /locus_tag="IVN13_001318"
     CDS             complement(26667..27395)
                     /locus_tag="IVN13_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP7011814.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            complement(27554..>27713)
                     /locus_tag="IVN13_001319"
     CDS             complement(27554..>27713)
                     /locus_tag="IVN13_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7011815.1"
                     /translation="DDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVKTVQEA
                     LDYLEKMK"
BASE COUNT         8575 a         5391 c         4719 g         9028 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattcaatt tctgatatta acctgatcta ctcaggacaa caattaacaa tcccaacaga
     8641 aggctctgca caagcagctg ctgaacctca agcagccgtt caagaagcac cagtccaagc
     8701 tgaacctgtt caagctgaac aacctgttgt acaagaaacg gttcaaacgg aaacacaagc
     8761 agcgcctgtt gctgaaacac aacctgctcc tgctgtaaca gaaactgctg ctactccagc
     8821 aagtacaagc tctgcaaaag aatggatcgc acaaaaagaa tcaagcggtt cttacacagc
     8881 aacgaacgga cgttacatcg gccgttacca attagattct tcttacttaa atggtgacta
     8941 ctctgctgca aaccaagaaa gagtagcaga acaatatgtt acttcacgtt atggttcttg
     9001 ggaagcggcg aaagcattct gggaagcaaa cggttggtac taagattcat tatttgaacg
     9061 tttaaacaca ttcaaaatat agtaagaggt catgcgattt attctttcgc atgacctctt
     9121 tttgagttta agtttagtct ttattcctta actatcgttt gccagtattt cttgtatcag
     9181 aatctataaa tctactattt gaatcaatct attaggtttt aaactgtccc accttcacac
     9241 aaattttttt gatttatttg aatctggttc cgttgtttaa aatttttcct atattctttt
     9301 agataatatg ttattttctt tctcatcgct ccaggtgtac cttctcttta taaaaaaaca
     9361 aattatgcgg ctaagaagac tcgaacttcc acgaggtcac cctcactagc gcctgaagct
     9421 agcgcgtctg ccaattccgc catagccgca tcacatcttt tagtttagga ttacatgtgt
     9481 tttttgtcaa gaacatattt ttgttctttt tcatttctgt acgccctccc ttatgccatc
     9541 tacacaaata attcatccga gcaactccat catctattga gggttggtga aaaaatctgt
     9601 tttatccata tgatcgattt caatgttaaa cgaacgactc tgggattagt aactatttta
     9661 tttttttctt catttctttt ttcctctttt tctcgattca gctatcttct ttcatttgga
     9721 tattgattat ttcttctgtt gattactatc cttctggaat tctagacgct gaagtaattc
     9781 acgttttctt tcttctagag atcataagta acgaatgaac gaacttcaaa taattttttt
     9841 attgtaattc atatttgtat tgttcgatga ttcttaatac gatatgacac aataaataaa
     9901 aataggcatg gctatttaca acaatagtgc tatagtagaa aacaaatgaa taaggttact
     9961 taaagaggtg aatacgatga aactgatcaa actatgggaa gcagatttaa agaaagctta
    10021 tcagctacaa actagctttg gaccaaatga gagtggattt atcaacaatg cttatggcta
    10081 tacatttgct gaatttttag attatgtaaa aaaatgcgaa aaaacgtctt taggaatcga
    10141 tcttcctgag agttacgttc ccgcaaccgt ctttatttta gaaagtgatg aacatgagta
    10201 tgtaggaatt tttaatttgc gccataaact gaacgaaaca ttaagaaacg gcgctggcca
    10261 tattggttac ggcatctccc ctaattatcg taaaaaagga tatgccacac aaggattagc
    10321 attagtacta caagaggcaa aaaaaatcgg cattgatgag gcacttttgt ctgtaaataa
    10381 aaaaaatctt ggcagtctta aagcacaaca aaaaaatggg gctgcgattt atcgtgaaga
    10441 tgagaaagaa tactatacga aaatcaaact gatgtaaaaa agaaggattc caaaaggaaa
    10501 gggatttgaa tgtcctttat caatagaaca catttattta attaacttat aatatagcag
    10561 acaaaatttt ataaacaaag aaacctttta tctcttttct tctagaaagg ataaaaggct
    10621 tttttgtttt tcctatttgc ttctcatagc tagtcagcca agatttctat cgtatcccca
    10681 acgcaatccg tgcataacgg ctcatcttat ctgtcgtcca tgctggatac cagactagtt
    10741 tgacttctgc tttcgttact tctggcactt cttttaatgc gtcatggata gattctgtta
    10801 aaatatctgc taatggacag cccatagttg ttagagtcat cttaatgact gtttctccat
    10861 tttcaggatt gaattctata tcataaatca agcctaaatt gacgatgtcg attcctaatt
    10921 caggatcaat caccatttcc aaggcagcaa gaatacgttc tttgattagt tctatttctt
    10981 gtccagaccg ttcttggttt gcttcgctca tcgtacgaca ctccttaaca cgatttttat
    11041 ttatcatact ctttttttag ctgcttgtaa atgagaattt agaagtaaga ttctcgttta
    11101 aatttataaa aatgttctta ttagatgtaa tagattaaat ttattctaaa ataaacctga
    11161 ttcctcattt tttttgtgaa cgatttttta tttctggcaa tacgccagtt gttcgataca
    11221 ttttttgttc gttttcattt gcaaaccggc caaatactga atgagttggt aaaatattga
    11281 taaatgcatc gtcgtcgatt tcatgaacga tctgatcaag atcatacatt tcgtagcggg
    11341 tgatgaccat catgatcatg ttcccttgtt tttttgaata gccaccgaca gacggcagta
    11401 atgtcattcc acgtaccatc cgttttgcta gttcttctgt tacctcttct ggcttgacgg
    11461 taacgatcat tgctgttact ttttgatgac tcgtgtggat ggcatcaatt acttgagtca
    11521 ttgcataaat ggaaataatc gtatacaagg cactttccca gttaaagaag aagcctgcga
    11581 tgactacaat gataccattt agaatcagca tgtagtttcc gactgttttt ccagttgttt
    11641 gtgaaagtac aagagagatg atatccatcc caccagttgt aaagcccatt tttaaagata
    11701 atcctacgcc aagacctaat aaaacaccgc ctactagggc attcatcaaa atattatcag
    11761 tgatctgtcc gactggaagc agcatcgtca tcaccgatac acatacaaca ttcacaaaac
    11821 taaggatcgt tgctcgtttt cctactttga gaaaccctaa tacaaacaca gggatattca
    11881 aaagaaaaat aaatgttcct gtgtctatct tgatatgaaa ccattcatat aaaagcgagg
    11941 aaatgatttg agcgatccca ttcatccctg cagaaaagac attggctgga atcaaaaaat
    12001 aatttaaagc gaccgcagcg gtcaatccgg ttatcaaaat aaccactatt ttctttaatc
    12061 tttcattttt atgatacgca gtcataattt tactcacaag cacctctccc ctactttggt
    12121 attccaaatg taagaataac agtttatcta ttaaaataaa atacttcgaa ctcaaatgag
    12181 aagaatttta gcaaacaggt aagaatctgc cctaatccta tgataagata gatgtatggt
    12241 aaatatttat atgtatatca ggagggttcc tatggcaaaa gatgctagct tcgacattgt
    12301 atcagaagtc aatacagaag aagtaaaaaa tgcgattcag caaacaacaa aagaattgtc
    12361 caatcgcttt gacttcaagg attctacagt agaaatcaaa ttagagaaca gcaatctagt
    12421 tgtattgagc gatgatgatt tcaaaatcga acagatcaaa gatgtattat ttggtaagct
    12481 gatcaaaaga aatgtgccaa tcaaaaatat ccatttttcc gaatcagaac acgctctagg
    12541 tggcaaagct cgacaaactg ccgatttggt caacggaatc gatcgtgaaa acgcgaagaa
    12601 aatcactaca gctatcaaaa atgaaaaact gaaagtaaaa gcacaaatcc aagaagatca
    12661 gattagagtg acaggtaaga accgtgatga tctacaagct gtgattgctt tattacgtaa
    12721 acttgactta ccaatcgagt tgcaattcac taactatcgt taatcaaaaa acgaggctga
    12781 cgtctgtcat tcctcgtttt tttgatttac tgcttctttt gataaccaaa aacgctgtat
    12841 tccttcttcc aattccccaa cacttcgtac ttctcttgct gtttgccaat gttgtgggaa
    12901 taaacgtcga taagaaaaac tagaaaatcg ttgatctgcc aatacaacga ttccttgatc
    12961 cgccatgcca cgaatcactc gaccaccagc ttgcaagact ttgttcattc caggaagctg
    13021 ataagcgtaa gcaaatccaa ggtgttcctt ctcatcataa tatgatttga tcagttcctg
    13081 ctcatgattc atctgcggca aacctacacc tacgatcata ctgccgatca atcggtcccc
    13141 tctcaaatcg attccttctg aaaaaatccc gccaagtacg caaaatccaa tcaatgtttc
    13201 agcgggattt gtttgaaatt ttgttaaaaa tcgttcgcgt tcttcctcat tcatgtccgt
    13261 ttcttggatt agcacattgc tgtcgggata ttgctgtctg aatgcttcca ccgcaagatc
    13321 taaatattga taagaaggaa agaaaacgaa ataattcccg acctttccat cagcaaaagc
    13381 atgaatcgtc tctactaatt ttgccagact gtgttccctt tttcgataag tcgtctctag
    13441 gtaatttgcg atgaaaactt gctggtttcc ttctgggaaa ggactcggca gtttgtaagc
    13501 cagacttttt tgtcccccca gtgtttcctg ataataagag agtggtgaaa aactagcaga
    13561 aaaaagaata ctgctttttc ctttatccaa cgactgctca agaaacaagc ttggatcaat
    13621 acaaaactct tttactatca gatcgcgata cgttttttcg actgttgttt cgtagtgatc
    13681 atcataaaat tcactgacct tcaaaaaatg aagcaaatca aaataataag gaagcaattg
    13741 ttcttgtgat gggtgctcag gaaattctgc cagccattct ttaatttttt cgcttaattg
    13801 ataacctgcc tttactaaag attcagccgg tgctttttga tgatgataat cccaatgatc
    13861 ttcttttgct atttctcgga tcgaatcaaa ttcttttaat agtttgttga aacggcgatg
    13921 aagttttttg aattcctttg gtatagcttc cttacttcgt ttcgtttttt cataagatag
    13981 ttctgccgaa tacatctctc gggaacgatt caccaaatta tgtgcttcgt caatcaaaaa
    14041 cagatagtcc tcgttttttt catcttcaaa aaaacgtctt aaataaacag ttggatcaaa
    14101 taaataattg taatccccaa tgattacgtc acaccagata ctgacatcta atgatagttc
    14161 gaaaggacaa agtgtatgct ttcttgcata agtttcgatg acttcacgag tgatctgatt
    14221 ctcatgattc aaaagatccc ataaaccttc attaatccga ttgtaataac cattggcata
    14281 tgggcactga tctggattac aggtcgtctc atctaaaaag cagatcttgt ctttcgccgt
    14341 caaagtgaca ctttttactt cagctcctgt atcttttaat gctgtcatag catcttcagc
    14401 tacttgtcta gttattgttt tagccgttag ataaaagatc ttttctccct cttcttcccc
    14461 tacagctttc aaagcgggga ataacgtaga gattgttttt cctgttccgg taggtgcttc
    14521 tacaaacaat ttctgtttcg tacggatcgt tttataagca gctaccgcta gttcacgctg
    14581 acctttccta tactcatcat gggggaattt caaagccatc aaagaggcat tgcgtacagt
    14641 gcgccagttt tcttgaaaaa caagccattc tttataatct tcagtcagct ttttaaaaaa
    14701 agtttctaac tgctctcttg tctgatgttc aacctttctc gtgatcactt cttctgccgt
    14761 ctggaagtaa gtcaattgca gatttatttc ttgtaaatca tgttcttgac tatagatata
    14821 ggcatacacc cttgcttgat gaaagaaaag ttccacttgt tctggttcta aatcttcaaa
    14881 tcgtggttcg gaggttttga tttcatctat ataataaata ccgtctcttt tgaaaatacc
    14941 gtctgcacgg ccttcaacga tcaggtaata ttcatctaat tgaactgttg tttttaaaaa
    15001 gacttctttt tcatagtttt ctcccgcatc tttttggagc ttgcggtgga ttctcgctcc
    15061 ttctaatgca gtatggttac tttttttacg attgtcgata cttccacgtc gcaaaataaa
    15121 ttctactaag tgccgcacag atatttttga ctgtttgatc atttccacca cctctttctc
    15181 tctaagatta cctgaaaaaa cctagtttag caaaaagagc tctctttcca aacataaaaa
    15241 aacaccctgc tgattaggca gggatatagg atatagaatt tggatttttg gatgaatcta
    15301 atgtaacaag gacatattaa aatgctatga aaacttactt tccttctctt tagaatatcc
    15361 aaatactttt catatagtct gataattcag ataagtaaag agaaataagt acctgttttt
    15421 gtttcctagc aatgaatgcg ttatactaat ggatgaaaga agttttggct atcaaggagg
    15481 aatgcacatg catatcgaaa gattaaaaac aggaatgatt gaagaaaact gttatttggt
    15541 ctacaacgaa gaagcacttt tgatcattga ccctggagaa gatgcggaac gaatcaaaca
    15601 agaaatcaaa aaaacaaacc aacagccggt agcgattttg cttacccata ctcactacga
    15661 tcatatcggt gcagtggaac ctttacgcgc gtattatcaa atcccagttt atgtcagccc
    15721 actagagcaa aaatggctag gtgatccgat tttgaatctt tctggactcg gtagacatga
    15781 tgatattgct gatgttattg tctcgcctgc agaatatgaa tttgaaatga aaacttatac
    15841 tttaggaaac atgtcttttc gcgtcgtccc taccccaggg cattctatag gaagcgtcag
    15901 ctttattttt gatgattttg tcgtttctgg cgacgccctg tttagaggaa gtatcggtag
    15961 aaccgatctg tatacaggga atttagaaca attacttcat agtattcgga cccagctgtt
    16021 tgtattacct gatgaatttg cagtctatcc aggacatggc gatgcaacga cgattgaaca
    16081 ggaaaaaagg acaaatcctt ttttcaatcg ttaaaacgac ttaggaggag aataaaatga
    16141 cagaaactac actttatatc gtacgacacg gaaaaacgat gttcaataca attgaacgag
    16201 tacaaggctg gtgtgataca cccctcacaa agcaaggaca agaaggcatt cattatttag
    16261 gtcgcgggtt aaaaaatgtc gacttttcat ttgcttattc tagtgacagt ggtcgtgcaa
    16321 tcgaaactgc ccgtattatt ctaggagaac attctaaggg aaaagaaatt ccttatttta
    16381 ttgaaaaaag gattagagaa tggtgtttcg ggtctcttga aggtggatat gacatggaaa
    16441 tgtggggtgt gatccctcgt gtattgaatt ttaaaacata tgatgaaatg tttaccacag
    16501 aagtttcttt tgaacaaatt gctaatgctg tatacaactt agataccgct gggtgggcgg
    16561 aaacttatga aacattgcga gaccgtgtat ggacaggatt tgaagatatc gcccatcact
    16621 gcgaaaaaaa tggtggtgga aatgttttag tcgtatctca tggtttgacg atcgcctttt
    16681 tgcttaatct gatcgatcct aaacagcctg ttcgtgctgg gcttttaaat ggcagcgtaa
    16741 caaaggtcac ttatgcaaac ggtcgatttt ctatccacgg catcaatgat acgtcttata
    16801 tcgataacgg gaaaacacat agtcgcgact cgtttctata atggggatat tttaattttt
    16861 accagagaat cataaaaggc aaagtctgaa cgagggcttt gtcttctttt ttctcttcgt
    16921 gtacttatct gctttatttc tcaagttttt cttaatatta aaaaacagat tgggtttctc
    16981 aaaaaataac ggtatactat aagatgtatc ttaattggga aggaaggaat ttatgttttt
    17041 tgcaaatatt atcaaagcag tgattctcgg tattattgaa ggaataacgg aatggctgcc
    17101 catcagtagt actggtcatt tgatcctagc agatgaattc atcaaactgg atgccagttc
    17161 acaatttatg tcgatgttca atgtcgttat ccaattaggg gcgatcttgg ctgtcgtcgt
    17221 tctttatttt cacaagttaa acccattttc tccaagtaaa tcaacggtag aaaaaaaaga
    17281 tacgtggaca ctttggttta aagtgatcgt tgcttgcata ccagccatca tcattggttt
    17341 gcctttggat gattggttag aagcaaattt ccacaagttc ttaccagttg ccattgtttt
    17401 gatcgtttac ggtattgcct ttattattgt cgaaaaacgc aacaaaaaca aaacacctcg
    17461 ttggacttct ctgaatgact ttacgtacca agcagctctt cttgttgggt tattccaagt
    17521 cttggcactt gttcccggaa catcccgttc tggcgcaacg atcctaggat ctatcttgat
    17581 tggtgcttcg cgttttgtcg ctacagagtt ttcatttttc ttagggattc ctgtcatgtt
    17641 tggcgcaagc ggcttgaaga tcgtcaaata tttagctgaa gggaattcat tcaaaatgga
    17701 agaaactgtt gttcttcttg taggaacatt cgtatctttt gtggtttccg tcttagctat
    17761 caaattctta ctaagctatt tgaagaaaaa cgactttacc gcttttggct ggtatcgtat
    17821 tatccttggt atcattttga ttggttattg gtttattgca atgtagaaaa aaccagaaca
    17881 cttctcttat ttgagaaatg ttctggtttt ttattttctt taataataat cattataaat
    17941 aataaaaaca acatcaagct taatcaccaa atgataacca ttatcgttct catttagctg
    18001 ttattattat caattacatt tttattatgc tatccttgtt ttgacaagga ggtctaataa
    18061 cgatgactaa tcagaaaaaa gcttttgcag caacaatcgg ctgcctttta tttatgatct
    18121 tcggttttat cttcgaccga ttctctctta ccatcacccc acttttttat atcagtgcca
    18181 ttatcatcgg tggatataaa caaacatcgg aaggcttgaa agaattgtgg aatgatcgga
    18241 cactgaatgt ggatttattg atggctttag ctgctattgg tgcctgtttg atcggtaatt
    18301 actttgaagg agcaatgttg acattcatct tttgtttaag cggtgcatta gaggaataca
    18361 caactaataa aagtcaaaaa gaaattactg ctttgatgaa tcttcaacca caaaaagccc
    18421 aattattaaa agaaaatgga caaatgcaag aagtagatgt ttctcaacta aaggtggata
    18481 acttggtatt tgtcgctaaa ggagcagctg ttccgataga tggcttttta gaaagcacac
    18541 aggcaacgat cgatgagtct gcattaagcg gtgaatctgt gcctgtagaa aaatatttag
    18601 gcgatgagat gtatgcagga acgctgaatc aaggaaatcc catcacgatt cgagtaacaa
    18661 aaacgagttc cgaaactgtc tttgctaaga ttatccaatt agtagaagaa gcgcagaata
    18721 cgccaactca aacagcttca tttatcgaac gaatcgaaaa taattatgta aaattgattt
    18781 tgcttgctgt tcctctaatg atccttctcc cccacttttt cctaggttgg agttgggatg
    18841 aaagctttta tcgaggaatg gtattattag ttgtggcttc tccttgtgcg cttgtcgcat
    18901 ctgctacacc agcttctcta gctgctcttt caaattctgc aaaaaatggg attttaatca
    18961 aaggcggtat ccatttagaa aagctttctg aattaaaagc gattgccttt gataaaacag
    19021 gaacgctcac taaaggaaaa ccagttgtaa ctgacagctt tttctttgat gaaaaggatt
    19081 tggcacaaca acttcttgtt gctatggaac aaaaaacgac gcatccactt gctcatgcga
    19141 ttttgcaata tttcgattgc acaatacctg aagaaatcca atacttacca atagaagaaa
    19201 ttactggttt tggattacaa actacctatt taggtgcgca atggaaagtt ggcaagcacg
    19261 catatgatcc tgagacgatg ataatcagca aagagattgc ggaaatgatc gaacgtttgg
    19321 aaaatcaagg caaaaccgtg atttatcttt ctaaagatca gcagttaatt gctgttttag
    19381 gtctgctaga tattccaaaa gcaaataccc aacaagtaat ttcctacttt aaatcccaaa
    19441 acatccatac aagtatgata accggcgacc attctggcac agcaaaggcc attgctgaac
    19501 aagtagggat tgaacattac tatgcaagct gtactccgga agaaaaaaca caactagtca
    19561 aaaaagagaa tgaactttac caagtaaatg cgatggttgg tgatggagtc aatgatgcgc
    19621 ctgctttagc agccgcttct ttaggtgtcg ctatggggca agggacagat gccgctatgg
    19681 atatcgcaga catcgtctta atgaaaagcg acttaaataa atttgtctac agtcatcggc
    19741 tatctttaaa aatgcggcgg atcatcaaac aaaatattat tttttcgatt cttgttattc
    19801 tcttgttgat tgcttctaac ttcctgcaat ttttaaatct acctcttgga gttgtaggac
    19861 acgaaggaag cacaatattg gttattctga atggcttgcg tttactaaga cctttacctg
    19921 atctttctga ttcttcaaaa aaatgtaagt cttgtccact atacaaagcg cggctgtctt
    19981 ctgtcaaata agcaaagtgt ttttttatga ttttgtttat tttatgaaaa aaacagctta
    20041 tgaataggtc ttgaaggatg aattcctact tttgtaactt tctaaaaaca tgttatgatt
    20101 gtttcgaaga aagtgaggaa tatatttcat ggaaagttgg ctttttttag gaattatttt
    20161 acttgtagca attttagcta aaaatcaaag tctgatgata gcggcagctt ttgttttgat
    20221 tatgaagctc ataccagcct cccgttcctt tttcccttgg cttcaaggca aaggaatcaa
    20281 tattggggtg acgatcatca cagcggctat tctaatccct atcgcaacag gagaaatcgg
    20341 tctacgtgat ctattagaag cctttaaatc ccctattggc tgggtagctg ttttttgcgg
    20401 tgggctagtg gctgtcttat cttccaaggg tgtaggattg attgcttctg atccacaaat
    20461 cactgttgca cttgttttcg gcacgatttt aggtgttgtc ttcttaagag gaatcgccgc
    20521 cggaccggtc attgcttctg gtatcactta ctgtatctta caagtatttg ctttaggaaa
    20581 atagaacaaa ttttgtctat tttaggagat tcttaacacc cccaatttac tgatccgtga
    20641 tatactgaca acgaggtgat acaatgttcg aaaaattaaa gtatttcata ggaacaatcg
    20701 gctggtttct tctagcggta cttttgattc tgggcttagc ttatctatta tttcctgatt
    20761 tgatacaaaa tgtgtttgat actgcttttt gattgaccta ttaaaacgat ataggggatg
    20821 ctcctgcgat gcaggggctt tttttaatca atgttttgta ataatttagg ccgtgccaat
    20881 gagacttcct atagaattcg atgctctttt ttctttttgg ctcttctgct cttttttgtt
    20941 atactagatt acagtggaca aaagaaagaa tgatcgcatc cacttcaacg aatatcaaaa
    21001 tggaagaatt agtgcctgtt ttcaggttac tttgaaaaga aaggaagacg cttgtggaaa
    21061 cttttgaagg tcttttaatt gatacgtacg ttttacaaga acagccaatg atgctgactg
    21121 ggatcaactt gcagcttgag gaccgtgtga tcaatggcct gcttaaagat cgctatttgg
    21181 caaaatatat cttacgtttg cccctaaata aatatgtgct ggaagtaagt ggcaaatgga
    21241 accgtcggaa ccagttggtc ataaaaaaaa tgttgataaa aaatatcgat gaatacatta
    21301 aaataatcgg tacaccagaa tagcctttac tgatcaaagg atactggtta taaaataact
    21361 gcagtttctt aatagaaaaa caaagaacaa cagggaatcg tatccctttt caaaaaaaac
    21421 tttgcaaacg gttctctttg ttatataata acttagtacc aaacattccc attcaaactt
    21481 tgtttaaata gataaataaa aatctagttt ggtttgacgc tattgcctcc cacaatagcg
    21541 tctttttgat ttttttcagc ttgtactatc cgtttttact gaaaaaaaat cctaataaaa
    21601 gactgttaag gaaaaaaact tgtcatgttc atattgcaaa gcacgctgcc tttaggtata
    21661 ataatgaatg ttgaattatg cggtaacacg cgtaagaact taagttatcg gaggtgaaac
    21721 taatgccaaa cattgaatct gcaatcaaac gtgttcgtac aagcgaaaac gctaatgtga
    21781 aaaattcatc tcaaacaagc gctatgcgta ctgcaatcaa aaaatttgaa gatgctgtag
    21841 cttcaggtgc tgacaacgta gatgcgttat ataaagaagc tgttaaagct attgatatgg
    21901 ctgaatcaaa aggtctaatc cacaaaaaca aagctaaccg cgacaaatct cgtttaagca
    21961 aaaaaatcgc taaataaaag aatcggcttc ggccgatttt tttatttgtc aaaaaaagga
    22021 caaaaacaaa cgaccttccc gcatgctttg ccctcgtctt tcttcttatc gatttcttaa
    22081 caactgccct gtgcgcaaaa cgaatagttg aaaacttaat tctttttcga tttgtcccgt
    22141 tttgatttga taatccatct caactaattg atcaaacaac tgtaccagca aatgttcatc
    22201 gaattttttt acttcctgca tggcaagttt tacacgatat ggatggattt tcaatgtttc
    22261 agcaatattt gcctgctgat aaccaatctt tacaagaaat tttgtctgta aaaataaacg
    22321 taattgagaa agcaaaatcg cattaatttt gattgtttct tccccttgca taactaaatc
    22381 ttcgtataaa cgcaaagctt gttccgtctt cccttttaaa atatattggg tcatgtcaaa
    22441 aatattatgt tctaacgttt taggaactaa ttcttctact tctcttaccg tgatttgctg
    22501 ttgattttgt ccataaagga ccaatttctg taactctctc atcattttgg ataaatctaa
    22561 gtcggtcaac ctcaagaata aatcaattgc acttcgttcc attttgattg aggaattctc
    22621 taaggtattc atcagatatt gacgtacttc tttttcattc aactgttgga cattgatgat
    22681 aattgctgtt tttttcaatt gtttcgttac ttttttccgt tcatccaact tttcatatgg
    22741 cgcaaagaat actaatacag tcgattctaa tggttctttc aaataagccg tcaataaatc
    22801 agtattttgt tcgattccat tgtttacttt ttcacctgtt aaaaaataag gattctctac
    22861 gaatactaac cgttgatctc cgaaaaacgg caacgtctct gcttcagcta ctacctcatc
    22921 taaacttgca ttttccatgt caaaacttaa aaaatttaag tcatcttcgc cctctaattg
    22981 gatttttttg acaatttctg tccgaacttg atcttggaga aacttttctg ttcctaaaat
    23041 caagtaacaa gatgcaagtt tttctttacg tattttttgt aactctgctt gtatactcaa
    23101 attactcacc actttcaaaa attatcctac catgagcaga tgagaattac tagtctatca
    23161 ttgtttccac tagcccatga ggatgaaaaa ctggatgcca gcgataggtg atcattcctt
    23221 gttgatccgt tcgccatgtt tttatctgat tttcttctag tgtctccaat acttcgctgt
    23281 gcgggtgacc ataacgatta tttacgccgc aggatataat cccatgtttt gcttcaatat
    23341 gggaaagaaa atctgtgtta cttgacgtac ggctaccgtg atgacctaat ttgactacat
    23401 cggctttcag attaggataa atcaaaagca attgtttttc cattgcttga ttcaaatctc
    23461 ctaaaaataa aaagcgccta ttgttatatt ccatccatag accaatcgaa tcttcatttc
    23521 ttccctctcc cttttcaaaa ggagtcaata cataaagatc ataataaccg ctgatcctgt
    23581 ccccatgttt gatttcaatg atctcagtac catttttttg agcttcagat aaatgccttt
    23641 gtaatggctc atttcgtcca gcgccccaac cgatataaat ttttttgata gtaaaatgct
    23701 gagataacgt gggtaattct cccacatgat ccgtatcgtc gtgtgtcaag atgagtttat
    23761 cgattacact tatcccctga ccttttagat atggaacgat atttctttca gcaggaggac
    23821 gtatcatacc ttgcgcccac ttttctcttt caaagtgtaa ctgacctcca gtatcgatca
    23881 aaatcgtttc ttttcgaaaa ggactttgca aaaagatact gtctccttga ccgacatcga
    23941 taaaagcgat cgttgaagaa aagcgtaaaa atggcaaagt caaaaaaata aaagaaaaaa
    24001 gaccaacata aaacaagtgt ttcacaggtt gttctttcaa cctatccata caaatcagta
    24061 atccgaaaat atacatccca gatagcacaa ggggcaaacg tcctataatg agagacgacc
    24121 ctgaagaaaa atcgattatt cgctcaaata agattaatcc ctcttctatc aacaaaagta
    24181 aagtttccgg cagccagcga ttgaagagtg tgagaaataa cacaccagga agaaaaagaa
    24241 acttgaatac cggtgcaatc agtaatgtaa aaagactacc taaaataggc cactcataaa
    24301 aatgccatac aatcaacggc atcgtgatca tagtaaacag gacgtgagag gccgtttttt
    24361 tcaaccaatc tccatcaaaa gaaatcagga taaacaaaaa actcatgaaa aaactaagct
    24421 gtccacccgc atgaaaaaga aatcgaggat caaatagtaa aagaagccaa aagacaactg
    24481 caaataagtc catggtggaa taatgcttat tcagcaattt gactagcaat cgcaataaaa
    24541 acagcaaact agcacgcaaa acagaaatag aacttcctgc aatagaaaca taacagatag
    24601 ttagtagact caacggaata atactgacgt gtagtggcca tccgatacgt cgaaataaat
    24661 aatacagcca ccctaaataa aattggatgt gtagacccga tagactgaac agatgcagca
    24721 aaccactctc cctaaaaatt tcttctggtt ctctgaatcc tgcatcttta tagccccata
    24781 ataaagaatt aatatataca cttgtttttt gcggaaattc agattggaca aaggagataa
    24841 aagatcctct ttttctctga agccagtttc gccagtgatt ttgcatggtg atctgttcaa
    24901 tcttttgaat ttggaaccgt ccagaaattc tatggttcct gaagtaagct tcttggtcaa
    24961 aattataaag attccgcttt tttgctggag agatgaactc gccttcagca gtcaattcta
    25021 ttccctttcc tcggaagtta agccatgttt tcaattctgc ttctgtgttt acttgataag
    25081 aaagctggag cttttgttta ttctctgata accttccttc cattgtcaaa aaattaccat
    25141 ttgcacgaat cgtatctgta aatacagtaa tttgcgcaga tgttgttttt tcctctaaaa
    25201 atgaatactc ttgtagccta taagaaatgg ataccgttat gctacacaaa atacacaagt
    25261 acgcgataaa acgtccttct tttagcgaaa accataaaaa aattccggca agaagcaaac
    25321 gataacccca attgtcagtg taatacaaca aacagataat catcgacgta attgccggaa
    25381 aaatcaaccg atttcgaaca tgcctttcta aaacgctaga catgttttcc tgttaattca
    25441 ttttgaagtt ttgaaaaatg ctgtgagtct aaagcaactt ggtgtacaga tacacctaat
    25501 tgttcgatca acgcttgggc ataaggatca tttcgatagt catggagata attgatccct
    25561 ttcacaccag cttgcaacaa agcctttgta caggccaaac atggaaaatg tgtgacatag
    25621 atttctgaac cttccgtcgg gataccaagt tttgcacact gtaggagtgc attcatctct
    25681 gcatggatcg ttcgcaagca gtgtccatcc acgacgtaac acccttcatc tatgcagtgg
    25741 acatctccac tgactgctcc attatagccg ccagcaatga tccttttttc tcgaacgagt
    25801 gtcgctccta cttctaaacg tgtacacgta ctacgtaaag ataacagtac tgcctgagcc
    25861 ataaagtact ggtcccaagg aatgcgttca ttatgtgact tatcagtaac tttttcttta
    25921 ttcgtatcgc atgcttttga agatttttct aacatgtttt atattcctcc gttttttcaa
    25981 gttgattaaa aagtactcat tgcttaaaca aaatattctt taaatataac gaatcatcct
    26041 gtctgacaaa agatcctacc cttcactgcc gaaatgaagt ccgtcatcca ttagcccatt
    26101 ataaaaattc ttcgaatcca tttgttcaag aaattccgta taaaaatcga tacttcacaa
    26161 tcatggcgtt ttgctacttc tgtgagaaca gagatatatt ctttgatcaa cttgttattc
    26221 cgatattttt gttttgtctc atcgactgct ggtggcgtga taaagaaagc atgatcagaa
    26281 ctatagtctt ttttcagctg aactatcatc cactgcagat tttttggtac gtttctttag
    26341 aaagttgttt atgctctgct agatcattcg tcccaatgag aataaatact ttatctgctg
    26401 tatagctaag tacatctttg gcaaagcgtt ctcgtagagc ttgactgtta ttgccgctga
    26461 cagccgtatt cataatatga atcccagata ttttattttg gagatagaca ttcagcattg
    26521 gtttttttaa tgtttctttg cgaacaatca agctatttcc tatccaaaaa tactacctcc
    26581 tcgtcctgcc aagctcttag aattggatta ttcttagtat ccattagcgt ccatttattg
    26641 ctcgtgccag tcgattttga atcattctat ttaattgtaa cgaagttttt tagtttttca
    26701 actgtctttt caccgatacc agaaatctcc tgcaggtctt ccactttctg gaaggatccg
    26761 ttttcttctc taaatcgaat gatttcttgc gctttgactg atccgatccc aggaatctgc
    26821 tgaagttctg atggagtggc tgtattgata tttattttgg gaggctcgga atctgctgta
    26881 ggctgttttc cttccgagga tgccaactga tcaagatgtt ctggcagctc ttcacctaca
    26941 gcagggatat ataggacttg ctgatcttct atcttcgcag aaaagttgat tcgatcgcta
    27001 tccgcttctt ctaataatcc acctgcacgc tttagtacat catagacccg ttcttctgaa
    27061 gaaaaggaat agataccagg ctccttcaca cttcctttga tatcggtgta catgaccttt
    27121 ggacgagcat cctcttctac atcgtttcct tcagctgatg gatcttctcc actggaacta
    27181 aacgaagaaa gtatttcttt tgcagcagtc tcttctgttt ctctataaaa tagcaaattt
    27241 gataataaaa aaccacttgc gatacaaaag ataagaacta tcatagacat ccaaatgatc
    27301 aatttgggtg aagctagtat tttcttgctg attttttcta tcattgttct tttttcaaag
    27361 aatgaccagt aaaaatcatt cattactcct ttcatttaaa attgaagaaa cagtgagctc
    27421 tggcaaaatc gtcccttcaa catctaatta cgtcaatttt ttcttttgtt tttattttta
    27481 tcgtgaagat ttttgacgaa aaagaggggt tgtgacagaa ctcgtgtcac aacccctctt
    27541 taattattta gtttcatttc attttctcta gatagtcaag cgcttcttgt actgttttta
    27601 ctggtacgat tttcattgag gaattcaatt ttttagcggc agcttttgct tcttcgtagt
    27661 tggtttttat ttcaggatat tctttcttaa catcgtctga aatcgtatca tct
//
LOCUS       DACCKU010000033        27406 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.035, whole genome shotgun sequence.
ACCESSION   DACCKU010000033 DACCKU010000000
VERSION     DACCKU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 27406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.035"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            153..1859
                     /locus_tag="IVN13_001320"
     CDS             153..1859
                     /locus_tag="IVN13_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP7011816.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="IVN13_001321"
     CDS             complement(2017..2919)
                     /locus_tag="IVN13_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7011817.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3620)
                     /locus_tag="IVN13_001322"
     CDS             complement(2916..3620)
                     /locus_tag="IVN13_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP7011818.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="IVN13_001323"
     CDS             complement(3651..4292)
                     /locus_tag="IVN13_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7011819.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="IVN13_001324"
     CDS             complement(4330..4644)
                     /locus_tag="IVN13_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011820.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5261)
                     /locus_tag="IVN13_001325"
     CDS             complement(4632..5261)
                     /locus_tag="IVN13_001325"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP7011821.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="IVN13_001326"
     CDS             complement(5268..5984)
                     /gene="pyrF"
                     /locus_tag="IVN13_001326"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP7011822.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            complement(5981..6913)
                     /locus_tag="IVN13_001327"
     CDS             complement(5981..6913)
                     /locus_tag="IVN13_001327"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP7011823.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="IVN13_001328"
     CDS             complement(6910..7686)
                     /locus_tag="IVN13_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP7011824.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="IVN13_001329"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="IVN13_001329"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP7011825.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /gene="carA"
                     /locus_tag="IVN13_001330"
     CDS             complement(10930..12006)
                     /gene="carA"
                     /locus_tag="IVN13_001330"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP7011826.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="IVN13_001331"
     CDS             complement(12011..13291)
                     /locus_tag="IVN13_001331"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP7011827.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="IVN13_001332"
     CDS             complement(13310..14236)
                     /locus_tag="IVN13_001332"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP7011828.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="IVN13_001333"
     CDS             complement(14249..15532)
                     /locus_tag="IVN13_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP7011829.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="IVN13_001334"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="IVN13_001334"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP7011830.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="IVN13_001335"
     CDS             complement(16320..17231)
                     /locus_tag="IVN13_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP7011831.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /locus_tag="IVN13_001336"
     CDS             complement(17228..17692)
                     /locus_tag="IVN13_001336"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP7011832.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="IVN13_001337"
     CDS             complement(17697..18395)
                     /locus_tag="IVN13_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7011833.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="IVN13_001338"
     CDS             complement(18382..18768)
                     /locus_tag="IVN13_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011834.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="IVN13_001339"
     CDS             complement(18915..20585)
                     /locus_tag="IVN13_001339"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP7011835.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="IVN13_001340"
     CDS             20768..21001
                     /locus_tag="IVN13_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP7011836.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="IVN13_001341"
     CDS             complement(21100..21531)
                     /locus_tag="IVN13_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP7011837.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..22023
                     /locus_tag="IVN13_001342"
     CDS             21610..22023
                     /locus_tag="IVN13_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP7011838.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22061..23209)
                     /locus_tag="IVN13_001343"
     CDS             complement(22061..23209)
                     /locus_tag="IVN13_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP7011839.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23331..23927)
                     /locus_tag="IVN13_001344"
     CDS             complement(23331..23927)
                     /locus_tag="IVN13_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7011840.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="IVN13_001345"
     CDS             complement(24127..26769)
                     /gene="alaS"
                     /locus_tag="IVN13_001345"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP7011841.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27157..>27406
                     /locus_tag="IVN13_001346"
     CDS             27157..>27406
                     /locus_tag="IVN13_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7011842.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKG"
BASE COUNT         7728 a         6056 c         4826 g         8796 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta acggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcagata atcttgcacg tcaggctctc cataaacaat taaaagaaaa aaagactctt
    22021 taaattaaaa actttaagga gtctttttat agtttctcat ttaatataaa agataattgt
    22081 gatcaaacgt aatttccaat tggtaatctc cgattttttc ttctatttga ctacgtaaga
    22141 ttctttctag ttctgtatca acaaactgga atttctcagg gaccactaca tcaaattgca
    22201 gcgtttttcc ttgatgaatt actctaaaat catgaatatt aagccctgta cctgtttcaa
    22261 gaagaatttg ctttaactgc cgcaagatat tatagtattt ttcgttatca acaggcattg
    22321 gatccaagtg acagacaagt tccacatcca aagacttttt aaagtctttt tctatcagat
    22381 caataatctt atgagccaca ttcaaattca ttgattcatc tacttcgatg tggactgatg
    22441 caaagcgttt attgggtcca tagttatgca ccaataaatc atgatagcca agtatatcat
    22501 cgcaagcatt taaatgctct tccatttctt caatctctgc tggcgttggg cgacttccta
    22561 atagttcgta tacaaaattt cgaagcatcg tcaatccact ataaaggata tataatgcta
    22621 aagcaaaacc tatatatcca tcaatacgcc atccagttac ccattcgatt gctgctgaaa
    22681 gcaatacagc cagtgtagta aagacatcat tataactatc tgttgctgta gctctcaatg
    22741 tttcagaatc aatggattgc gcaataacct gatacatttt gccttgtgca aatttcaaaa
    22801 agatggaaat gattaggaca aaaaagacga tgggtgttaa agagacattt tctggatgga
    22861 tgattttttc agcagaagac tctaaaaatt gaaatcctac ataagtaatg atgatcgaaa
    22921 caaataaccc actgatatat tcgaagcgtt cgtgtccata aggatgttcc tgatctgctg
    22981 gctttgcagc aattttaaat ccgatcaaag tcaatatgga tgaagcagta tctgatagac
    23041 tgttcactgc atcagccata atggagacac ttcctgaaaa aagcccgatc attagcttag
    23101 agacaaacaa gatcagattc gtaaccaatc ctaagatacc cgctagtact cctttttttg
    23161 tccgctgttg cggtatttgt ttcttttttt cttcttgttt cttagccaag ttttttaccc
    23221 cttctagttt gcttgttgct caggtaaaaa gatggagagg acgattgttt tcctctcctc
    23281 tcttcctttt tctatctatc gtgcgtccta ttactattaa aagatggtca ttattctggt
    23341 ttcaacggtg ttgctgcaaa taacgctttt gctagtaatg gtgttatgat tcccgcacct
    23401 attgcttctg gtatcccatt gattgctgca acccctgcta aggttttaaa taaaagagca
    23461 gagtctacac cataggcatc tgccgttgca cctgtataaa ataatcccat gaatcctaac
    23521 actaagactg tattgaccaa tgaacccagt acccctgcaa aggctgagct gatcgttatt
    23581 gatttatttt tttgataaat ccagtggaaa accaagcctg ctactaagcc aacaagtact
    23641 ctgggaacta ccgagataat cggattcgtg aacactaatg tatcaaatgg cgttgttggc
    23701 atggcatagg cacgaacaat tgtagcgatt ccccaaaaca agccaatcac catgccatct
    23761 tttgggccta atactactgc agcaataatt actgtgatat ggataattgt taaactaatg
    23821 aatcctaatg gaataaaccc cagccaagga accatcgctt gtacgatgat gattgctaac
    23881 aagatcgcac gtaagactag tcgaaaggtc ttatttctac tattcatttt ttcacccctt
    23941 tttatttata ctaatcgtaa aaatttctgt cactttcgtc aactaaagac catccgaatc
    24001 cgaccatttg attagatgcg ccatccaatt gaaaaaagta tgagggcaag acttgtttgt
    24061 cttcgccccc ataatcatgt ttcgttcaat gtttgaactt ttgtttttct gtcaaacgta
    24121 aacgccctat tgttgcgcaa cccattcttt cgcagcttta agtgcatctg gaataccagc
    24181 aggatttttc ccgccagctt gtgccatgtc tggacgtcca ccaccgccac cgcctacttt
    24241 cggtgcgatt gctttaatca agtcgccagc ttttagtcca gcttcatttg attctttcgt
    24301 cattgccgct agcagactga cttttccttc ttgttcgttt gctaaaacaa gaatatctga
    24361 aatatttttt tgtttccatt ggtcagctaa ttgacgcagc tgattcatat ctttcacttt
    24421 tacttgtgct gcaatgattg tttttccatt gatcgtttct ggatctttga atacatcacc
    24481 agcttgttga ttcgctaact tgctagctaa ttgttcattt tccttttgta gctctcttac
    24541 ttgctgttgc aattgttccg cttttgaaac aacttctttc aattgaggtg atttaacaat
    24601 tgttgctact tgttttagtt gtttttcttc ttcttcaagt agttcgtacg cttctttact
    24661 tgttacagcc tcgattcgtc gcacgcctgc accaattcct gattcagaaa cgattttgaa
    24721 gattccgatg ttttctgtat ttgctacatg tgtgcctccg cacagttcga ttgaccagcc
    24781 gccaatattt actactcgaa cgtcatgtcc gtatttttca ccaaacaaag ccattgcacc
    24841 catgtttttg gctgtatcga tatctgtttc aattgtttca actgggatag cttcccagat
    24901 tttttcgttg acgattcttt ccatttcagc taattcatct gaagtgattt gaccaaagtg
    24961 ggtaaagtcg aatcgtaaat gtccaggagc gactaaagaa ccagcttgat ttgcatgctc
    25021 gccaagtact tctttcaatg ccttgtgcag tagatgggtt gctgtatggt ttttgatgat
    25081 ccgattgcgc atcgattgat cgatcaatag atgataggtt tgtccttcac tcattggtgc
    25141 ttttacttct acggtgtgca taaattgacc attaggcgct ttttgaacat ctgtaacaac
    25201 tgctacgatt tcgcctactt cattcatgat ccatccttga tccgctactt gcccacccat
    25261 ttctgcatag aaaggtgttt gatcaaagat catttgagct tcaccttctg cgacttcatt
    25321 caaaagagac tcacctttaa cgatgaccaa taattgacta tctgattctt gttctgtgta
    25381 tccaatgaat ttactttcta ctttgatatc tgtcaataaa gcagattgta cccccattga
    25441 tttttcagta ctgcgggcag agcgagcacg ttcgcgttgt gcatccattt ctttttcaaa
    25501 accagcatga tcaactttca gtccttcatc ttctgccacc tcttccgtta attctaccgg
    25561 gaagccatat gtgtcgtata gtttgaagat gtcttttcct gctagagtcg tcccgttttc
    25621 gttttttact tcttggatca cttgatttaa gatttccaaa ccttcattga ttgtttcatt
    25681 gaaacgttct tcttctgtac ggataacttt ttcaataaat tctttttgtt gaagtacttc
    25741 aggataataa cttaccatga tttccccaac aacagggact aaacgatata agaatgcttg
    25801 ttcaatacct aattttttcc catgcatgac agcacgacga agcaaacggc gtaagacata
    25861 ccctctccct tcattcgatg gcaatgcacc gtcgccaata gcaaatgaaa gggcgcgaat
    25921 atgatctgcg attactttgt aagaaatatc tgttactggg ttttcaccat attttacttg
    25981 tttgcttagc ttttctacgg cgtggataat aggcataaac aagtctgtct caaagttcgt
    26041 tgacgcatct tgaacgattg agaccatgcg ttccaatccc atccccgtat caatgttttt
    26101 atgaggaagt ggttcaaatg tgttatcagg cttatggttg aattctgaaa atactaagtt
    26161 ccagatttct aaataacgtt cattttctcc tcctggatag ttttcaggat cgtcttctgc
    26221 taaatcgtta taagcttctc cacggtcata gaaaatttcc gtatccggac cacatgggcc
    26281 tgcaccgata tcccagaagt tgtcttctac atcaataatg tgatctagtg gcaggccaac
    26341 ttcttcatgc cagatgcgtt ttgcttctgt atcttttgga taaactgtta cataaagttt
    26401 ttctggatcg aatgccatcc attttggatc agtcaaaaat tcccatgccc agtggatggc
    26461 ttcatttttg aaatagtcac caatcgaaaa attacccagc atctcaaaca tcgtatggtg
    26521 ccgtgctgtt ttaccgacat tttcgatatc atttgttcga atagattttt gggcatttgt
    26581 gattcttgga ttttctggca caactgaacc atcaaaatat tttttcaatg ttgcaacacc
    26641 agagttgatc cacaataagg ttggatcgtt cacggggatc aaagacgcac taggttcaat
    26701 cgtatgtcct ttagattgga agaaatcaag aaacatttgt cgaacttgtg cactacttaa
    26761 ttgtttcatg tcattacctg ctttctataa attaaataca aaaaaacacc atcacagcca
    26821 aggacgaaaa ctcgcggtac caccttgatt gcaatgatgt gtaatcatta cctcttaaac
    26881 tcgataacgc tgagctgcgt cggtatttct accaaaatag aaaaagggag caatttgatt
    26941 agtgactatt cttttttcag ccaagaaaga attttctgtg agtcttaaaa aacaaatcat
    27001 atccttttcg tacttattta gtatacgcat tctattgatc actgtcaata tctcaatttt
    27061 tattttcatt tccttttggt catactccac gatttttggt acgataagaa taaaggaatc
    27121 aatacatctc tgctattcat gacaaggagg aatatgatga aaaaaatagt tggagcggat
    27181 gaatggatac gcgcagctag ctgctttttg agatatcaag tttttgtatt agaacaaggg
    27241 attcctgcag aactagaatt cgacggaaaa gatatcccgc aacaaaccta ttatctattt
    27301 cttgatcaaa aaagaccagt agctactttg cgttatgaaa agaaaacacc tacttgcttc
    27361 aagccggaac gattttgcgt agcaaaagaa taccgaggaa aagggt
//
LOCUS       DACCKU010000034        25895 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.036, whole genome shotgun sequence.
ACCESSION   DACCKU010000034 DACCKU010000000
VERSION     DACCKU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25895
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.036"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(178..585)
                     /locus_tag="IVN13_001347"
     CDS             complement(178..585)
                     /locus_tag="IVN13_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP7011843.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(774..1601)
                     /locus_tag="IVN13_001348"
     CDS             complement(774..1601)
                     /locus_tag="IVN13_001348"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAP7011844.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1910..2128
                     /locus_tag="IVN13_001349"
     CDS             1910..2128
                     /locus_tag="IVN13_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7011845.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2149..2784
                     /locus_tag="IVN13_001350"
     CDS             2149..2784
                     /locus_tag="IVN13_001350"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7011846.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2977..3216)
                     /locus_tag="IVN13_001351"
     CDS             complement(2977..3216)
                     /locus_tag="IVN13_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011847.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3240..4763)
                     /locus_tag="IVN13_001352"
     CDS             complement(3240..4763)
                     /locus_tag="IVN13_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7011848.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4781..4903)
                     /locus_tag="IVN13_001353"
     CDS             complement(4781..4903)
                     /locus_tag="IVN13_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP7011849.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4933..5916)
                     /locus_tag="IVN13_001354"
     CDS             complement(4933..5916)
                     /locus_tag="IVN13_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7011850.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="IVN13_001355"
     CDS             complement(5940..6089)
                     /gene="rpmG"
                     /locus_tag="IVN13_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP7011851.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6110..6487)
                     /locus_tag="IVN13_001356"
     CDS             complement(6110..6487)
                     /locus_tag="IVN13_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011852.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="IVN13_001357"
     CDS             complement(6519..6698)
                     /gene="rpmF"
                     /locus_tag="IVN13_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7011853.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="IVN13_001358"
     CDS             complement(6713..6982)
                     /gene="rpsN"
                     /locus_tag="IVN13_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP7011854.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7505..9277)
                     /locus_tag="IVN13_001359"
     CDS             complement(7505..9277)
                     /locus_tag="IVN13_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011855.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9231..10985)
                     /locus_tag="IVN13_001360"
     CDS             complement(9231..10985)
                     /locus_tag="IVN13_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011856.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11095..11664)
                     /locus_tag="IVN13_001361"
     CDS             complement(11095..11664)
                     /locus_tag="IVN13_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP7011857.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11682..13106)
                     /locus_tag="IVN13_001362"
     CDS             complement(11682..13106)
                     /locus_tag="IVN13_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7011858.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13108..13776)
                     /locus_tag="IVN13_001363"
     CDS             complement(13108..13776)
                     /locus_tag="IVN13_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7011859.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13907..14491)
                     /locus_tag="IVN13_001364"
     CDS             complement(13907..14491)
                     /locus_tag="IVN13_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7011860.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14822..15055)
                     /locus_tag="IVN13_001365"
     CDS             complement(14822..15055)
                     /locus_tag="IVN13_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP7011861.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(15070..16020)
                     /locus_tag="IVN13_001366"
     CDS             complement(15070..16020)
                     /locus_tag="IVN13_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP7011862.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16017..16859)
                     /locus_tag="IVN13_001367"
     CDS             complement(16017..16859)
                     /locus_tag="IVN13_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7011863.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16992..17717)
                     /locus_tag="IVN13_001368"
     CDS             complement(16992..17717)
                     /locus_tag="IVN13_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7011864.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIVHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18178..18492)
                     /locus_tag="IVN13_001369"
     CDS             complement(18178..18492)
                     /locus_tag="IVN13_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7011865.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18508..20103)
                     /locus_tag="IVN13_001370"
     CDS             complement(18508..20103)
                     /locus_tag="IVN13_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAP7011866.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20126..21016)
                     /gene="iolE"
                     /locus_tag="IVN13_001371"
     CDS             complement(20126..21016)
                     /gene="iolE"
                     /locus_tag="IVN13_001371"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAP7011867.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21033..22058)
                     /gene="iolG"
                     /locus_tag="IVN13_001372"
     CDS             complement(21033..22058)
                     /gene="iolG"
                     /locus_tag="IVN13_001372"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAP7011868.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22082..23089)
                     /locus_tag="IVN13_001373"
     CDS             complement(22082..23089)
                     /locus_tag="IVN13_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7011869.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23202..24362)
                     /locus_tag="IVN13_001374"
     CDS             complement(23202..24362)
                     /locus_tag="IVN13_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAP7011870.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24438..25892)
                     /locus_tag="IVN13_001375"
     CDS             complement(24438..25892)
                     /locus_tag="IVN13_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAP7011871.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
BASE COUNT         7945 a         5296 c         3974 g         8680 t
ORIGIN      
        1 aataagtaat ctaaccgact gttaactaaa ccattgcagg tcccgttaaa gatacttgtg
       61 cctgttgtca ccgcttcttt ttcttgtcca caagcgctta acacacttaa taaagcccct
      121 gctattatta ataataatat ctttttctta ttaaaaacct cctcatctat ttccagatta
      181 gtaaatgtag taatctttgc tttctttttt ctcaatacta ataactaact tatggggtaa
      241 acaaatactc gtttgaccgg gttttgagat ccatccagtt ctaaccgcga tttgatcagg
      301 actattatct tctttaactc gaattcttcc atctttaatc tcaacaatat tatattgtcc
      361 cttcgcagga tgataattca cgagtttatg attgatctta tccaaatcga agcgatccat
      421 ttccttgcca tcaatttgaa cgatggcata tttgatagca tcttcagttg tttcattctc
      481 cttttctata gagaatacaa ataagggaat aaaagaggga ataatcaaaa tgccaataat
      541 tatgacatcg cctatcttga caccagcttt tcttaaaaat ttcattaacg atatctggct
      601 aaacatatta gaatttttcc atggaaatgg tgaaattttt cggaaattat ctgcattaag
      661 agaatcagtt aaacaaccct caagttgttc cttaatacat tgttgatcca tcttcatacc
      721 aataaaaact agtttattaa tgcgttcacc aaacaacgga tgccaggctt ccttcaattc
      781 gggttttttt gtataatcat ttgacactca attttaggta aactagctac ccaataattt
      841 aatgattcaa tacttttatg gctacccgat gtttcaaaga tcgtcgaaaa atcactatgt
      901 tgggcaaacc aaagaaaccc ttttgctctt gtaatattgc caggaaaatt tttatccgta
      961 agatgggcaa atttttgtgc gtcaaggagt aaccgatttc gatagataaa acttgagata
     1021 ccatattctt ctgtttcagg aatatgattt tgatagccaa gctctaactc tttgaaccag
     1081 cccattgaat aggaagtctc atctatatta aaaagatttg tatttagaat tttcgataag
     1141 tctacttcac ctttaattgt cctaattatt ttggcattgg actgtaactt agcaattaga
     1201 ccgttaattt tgtaaatcgt cttggttgac actaagtcac tattatttaa aagtaatatg
     1261 ttacaacact cgaattgatc aattaacaag ccacgaatat cctgttcttc ttcaactaca
     1321 ttaccttcag cttttctttc ctgaatcttt tcacccgttt cgaaattatc ccaaaaacgt
     1381 tttacatcca ctactataat aatggcacca atctctaatc gttgggtcaa atcaatgtct
     1441 aaagggtttt tagctaatat aattgcttga ataattggaa ttggtttgct gattcctgaa
     1501 gcttcaataa caatttgatt caagtcagaa aattgcacta attgatctag ttcaacaatc
     1561 aaatcttcgc gtaatgtaca acaaatgcat ccattcggca tcgaaacaag tttttctcca
     1621 gttcttttaa aacctttctt gtcgactaat accccatcaa catttacttc ccctatgtca
     1681 tttacaatga cagcaatttc ctttccgtga tcaccgtaca gaatatgatt tagaagtatc
     1741 gtttttcctg aaccaagata gccagttaag attgtaatag gtattttttc atagggaacc
     1801 tctttctata ccttgaattt acactttaag tgcaacacat aaaaatgagt cataatcgat
     1861 atccaactag tataagtttg tcgagaacac tcctagaagg aaaataatta tgagccagta
     1921 ttgtcatctt actatactca aacgagaaag aatctttctg cttcacgaag aaggattttc
     1981 tattagtata attggcaacg acattcagca tagcccgttt actattttac atgaactgag
     2041 aagaaacaag aaaaaaacta atcaccttcc aaagcggaaa aaaattatgc ttatcgaaga
     2101 aaaaaatgcg gacgttatca cattctaaat gatccgtgag tttggtcaat tgttcgtcat
     2161 ttatttgtcg attttcagtg gtcgccagag aaaatctcga atcgtttgaa gcatgaacat
     2221 atggactttt caattagttt tatcacgatc taccgtgcaa tttgcaatga tcttttcgat
     2281 acagaacccc tctcacacgg caataaaggc ctgatccgtt cattgcacca tcgaggaaaa
     2341 acaagacata caaaaaatca tgtcgagtct cgtgggaaga tttgccttac tcattcgatc
     2401 catggccatc caaaatccgc agatgataga agtgattttg gtcattggaa agccgatatt
     2461 gtagctggca aaactagtgg tacttgttta gtgactttga cagacagaaa atcatgcttc
     2521 cttttagcag gaaagaaggt ccccagaaag aaagccgctt atgtgagcga gaaaatgatt
     2581 gaacttttaa gcagtattcc aaatgaggcg gtcaaaacga tcactccaga tagaggaaaa
     2641 gaattctcca atcatttcgc agttacagaa gtattgaata atatgccctt ttattttcca
     2701 gatcctcatg cccttggcaa caatgaacaa attaaaatac gaatagactc attcgagaat
     2761 acttgccaaa atcgaaagag atagctacgg tagataactc aacgataaat cagatgattg
     2821 aaaaactgaa taacagacct agaaagtgtt tagattggag aacaccttat gaagtcttct
     2881 ttgataaagt gttgcgcttg atttgacaat tcaagaaata ataaaaagca gatcaaatcc
     2941 aaataaggga aaaatgatct actctggtat tatcaattaa ttggccaagc tattatgccg
     3001 ttttgctaaa cgtctacttc ttccagtatt gaccttgata aaacaacaat caggatcccg
     3061 ttttaatact ctatgaactg tgtcaatact aacatgtaaa gttgcagcaa tatcagctaa
     3121 tgtactccca tttaatctca tttgctttgc aaattgataa cgccgataag catcaatatt
     3181 cttggaatcc atagacaata atacttttct taccatgtat ttcctccaaa gattggattt
     3241 taatgggcca tcatttcttg agcaatctca tgctcagata atgaagctgg atagaaagta
     3301 ggccaattat gcatttcatt ataaagtttt tcttgacttt ctccaccaaa gaaaatatgg
     3361 aaatgctctg ccttacttgg tgcaattcca tgatcgctaa attgaatata tttaaaacga
     3421 ccagcacctg catcatcagt ttcaaaatta aaacgaacac cacggtttcc tttttcataa
     3481 ttcaaaattt tgtatccaac atatttgtat gtgtattgat gatgttcacc gttgactaca
     3541 aaatcaatgg tgttgtcttt aatgttgata gtatcaatat ctgtcttata gccagtagta
     3601 taataatctt tgtactcggc agctgtcatg tctttattga gttttgcttt gtaatcgaaa
     3661 acttcatcca aaattccatc ttttaacaac ggataaacag attgccattc gcctgtataa
     3721 tcactcaatt cgcgatcctt cacatcggca tctttaaagt acccattagc aactgtcttt
     3781 tcatccttcg cttctttttc tggaactacc tcttcaccag caaccatagt tgttttctct
     3841 aacgctttta gattgtcttc cattactgaa acataatctt caccattatc catttgttct
     3901 tttgtaagac tttcaagagg attcaatact tccaatttta tcccagcttc attggccaaa
     3961 gtttttgcga ttttatcatt agcattcttt tcaaagtaaa tataattgat cttatttttt
     4021 ttaacatact cttttaattc agctaatcga ccagaagatg gctcttcttc aggacttagt
     4081 ccagcaatag gaacttgtat caatccataa tccaaagcta aatatccaaa ggctgcatgt
     4141 tgtgtcacaa aacttttttg tttcgcttct ttaagactag tggtatagtc agcatccaac
     4201 cgtttcagtt tagtcaaata cttttcagca tttgtctcaa atacttttgc ttttttcgga
     4261 tacaatttaa ctaactgatc cttgatattt gaaacttcct taattgccat tttgggtgaa
     4321 acccacgtat gaggatctag ttcatgatgg tgatcttctt ctccgtgatc atgatcttct
     4381 tcttcactac ctggcaataa aatcatgtct tttgttcctt ctactacatt aggcccagct
     4441 ttcttccaac tttctttagc ttttggtacc catgattcca tgttttcgtt atgatataca
     4501 aaaacatcag catcatgagc ttttgccata tccttagctg agggctcata gtcatgaggt
     4561 tctgatcccg ctggaaccat taaatccacg ttcgcttcat cgccgacgat gtttttggtg
     4621 aaatcataca ttggatagaa tgttgttaca atttctaatt ttccatcttt atcattcgat
     4681 acttttgcat cattagtgct ggagcaagct cccaataatc ctagagctgc tatcccaata
     4741 agtcctacac tgattttctt cattcttttt ccttccttaa ttatgctaat ttcttcattt
     4801 tattttgttt atgttctttg ataattttta atgccctttt tctagttttg atattcgggc
     4861 ttttaagtcg tcgatatgca ctagatatat ccgttttctc cattagaaat tacccctaat
     4921 caatacttat cattatttgc tttccgccag taatttatca aaactctgtt tgatcctttc
     4981 ttcatccaac atttttccaa ttagaataat agtggtttgg tctttttgtc gagtggtgta
     5041 ttgaaaatta acctgttcgt ttaccccttg tatggcaact ataaaatctt gatcttgcaa
     5101 agcaatcaat cctttaaaac gatacaggct cccttggttg gttaaaatca gccggtcaat
     5161 taattggaat aaaaaatctt tttttatgtc ttgatctgaa gttaagagaa ttgatttaaa
     5221 ctcatgttct tgatgatgtt gatgatagtg tttgccatta tagtgatttt ctgatgaatc
     5281 actttcaaaa actaagggat gattgaattt ttccaaatta taaaaatcct ttttttgaat
     5341 cgtttcacta tgtgtgaaca ttcgaatatc ggataaaggg ttaatacttt tgatttctct
     5401 ctgtaatttt tttagtgcag ataaattcaa gttttgtttt ctagacacaa aaatgcgatc
     5461 tgctatgaca agttgcttaa gggcttgtgg ttctctttca ataagttcaa aactgtcact
     5521 atcaataaca gtcaataaac tatcgatata tactcttcct cttagttgag gcgctgttaa
     5581 aagtgtttga atgattggtt ggggatctgc tatacctgta gtttcaatga tgatttgtga
     5641 aattggttgt tgatgttctt ctattgtttc cagtaaagaa atcaatattg ctaataaatc
     5701 actgcgaaga ctgcaacaca tacatccatt atttagttgt aacacccgtt cctcgctatg
     5761 aactaataat ttgtgatcaa tcccaacttc accaaactca ttttcaatta taataatttc
     5821 ttccctgggg tagggtgaga cttgcaaggc ttgattgatt agcgttgttt ttccagcccc
     5881 taaaaaccca gaaacgattg taactggtat gcccattttg tcaccttttt accacttagc
     5941 tatttcactt ctttgaaaat tgcccgtctg cgcaattttg gcgaatactt ttttaattct
     6001 aaccgttcag ggttattccg tttatttttg ctggtgagat acagacgttc tccagtttcg
     6061 acacattcca aaataatatt ttgacgcata aatcttcctc cttcaatact tatcgatctt
     6121 caagatatgc ttgaatttgt tgtgcatctt caaaagttaa ctcgccagat tccaattgtg
     6181 aaactaaatt ttctttcata tcaacaggta tctcccaata accatcaata cattgaaata
     6241 actcctcgtc atactcttca aaaattggat agattcttaa aaaatcatca aatgataagc
     6301 cttggtcctc atagtaatct ttgactaatc cattttcaat tgcctgttgg gtcggtaaac
     6361 ccgtttcagt aataattcct ctctctacca tcgaacgata aaaagtgcat ttatctagcg
     6421 attgctgggt cgtttcaaga taatacttta atccttcttc aactaaaggt gggtaaataa
     6481 tttccatttt gattccctcc taaaagttat atattaaact attttgaagc tcctttaatt
     6541 actttctttc cattatagta cccttttaaa gatacatgat ggctacgtct ataatcacca
     6601 ttcgcttcgt caaatgaaat ttctggtttt actacagttt gatgagttct tcttaaccgt
     6661 ttcttcgcct tagatgtttt tcttgctggt actgccatga tatttcctcc ttttaccaac
     6721 ttgctttttt tactccagga atcaaccctt gatgggcaag ttctctaaac ttaatacggg
     6781 acataccgaa ctttcgcata taccctcttg gacgaccatc cgtctgatca cgaagcctca
     6841 gacgattcgg atttgaatct cttggcaatt tagcgagagc ggtgcgatct cctgcggctt
     6901 ttaattcctg acgtttatct gcgtactttt caatcatcgc catttgtttc tttgctttag
     6961 caattttcga cttttttgcc atataccttt atactccttt tattttgatt tagacatctc
     7021 aaatcgtaat cgttacgttt tgacactgat actttaccaa caagagttgc tcttgtcaac
     7081 aataaataac atgaaataaa actcatatga atagataact atttgaaaac gaatttatcg
     7141 gtacatagag ataatcaaat catttaaaag aatatgcagt ttaagctctg aaaaataaga
     7201 attcactttt attagtccaa tcaaacacaa ataccgtttt aagtagcttt tcctctactt
     7261 aaaacggcaa tatgaaagaa actataactt tttattcaat cttttatgtt taactggttt
     7321 atcgccaatt ttttaaaact ttgattattc cttatattat aagaatagac attaagtgaa
     7381 ctaattcaat catactgatt ctttcgtgta ttcaaaatta gatgctgttt aataaaatta
     7441 ttctttttga aaaagaatta taactatatc ccaaaaattg taggttaatt taaagcctat
     7501 ttcatcatcg tgattgagct tctttttcat agacgaattt ctcagatagt ttagttttct
     7561 ttatgagatt tttgtatatt tctgaccttc gatacaactc actatgagta ccttgatcaa
     7621 ctatttttcc ttgatccatc actacaattt tatttacctt ttcaatagac ttcaagcgat
     7681 gcgatataat tagtactgtc ttatcttgta ttaaacgatt taaactttcc tgaatacgtt
     7741 tttcattttc cgcatctaaa ctggcagtaa tttcatctaa aattaaaatt ggcgcatttt
     7801 tcaaaaaagc ccgtgctatg gataggcgtt gtcgctctcc tccagaaagt gaacttccgt
     7861 tttctccgac caatgtatga taaccgtcag gtagtttttc aataaagtct tcgcagttag
     7921 ctaaacgagc tgcttttttc acttcctcat ctgacgcttg actatttcca atacgtatat
     7981 tttccaaaat acttgtatta aacaaagtta cctcttgaaa aacaatagcg attttgctaa
     8041 acaaagaagc tggagataca cgtttaatat catacccatc aattaaaata cagccttcat
     8101 cataatcata cagtctagac actaatttca agatactagt tttcccggaa ccactagcac
     8161 ctactaacgc agtcacttct ccttgtttag ccgtaaaaga gatatgatct aaaattgggg
     8221 tattgttatc atatgagaaa gagacatctc gcaactcaat gtcaaaagat ttcagaggac
     8281 tatctgatcc ctcttggata ctggtttcct tcatagctct aattcgctgt attttaggag
     8341 ctaaataaaa tatttcaagc actgcttctt tcatagaatc gaaggaatcc tttattttta
     8401 ttgcggctag taagtaacca acgacgtaga gtatagtcac ttcacccgtt aacagtaagt
     8461 gaacacctac tagaatgact actgctaaac taacatatga gaaaatagag gataaggcca
     8521 tgactgaaaa agtacttaat tctaccttta aatgaatcct ttcactctct tccatttttt
     8581 tgaaaagtct gtcttgaact tttttagata gattaaagct attaatctct tgctgcaatt
     8641 ctatagtttc ttgaaattct tccgagtttt ctctcaaagt atcgtaatat ttagtattgg
     8701 ctttcgtttg ggatttcttt gataacaaga ttaagacaaa actaaataac gttggcaaaa
     8761 taacagctaa tcccattttg acattaccaa ccaaaagcat cactgaaata aaagggaaaa
     8821 acagagccat accgccggct ttaggtattg catgactcat cgcatgctca ataccttcaa
     8881 cgtcagacat gatagtttga gataaatctg ataaattatg ttttgaaaaa taggataatg
     8941 gtaaatttga caaatcgtct gcaatttgca ttcttaaatg cgcactttct ttataagtac
     9001 tattatataa gttttcatac tccctatcta acaaccaata catgaaaagc aaagtaacga
     9061 acgatactgt aaaataaagc caaggcggct ttacattttc taaaactaat tcgtctatca
     9121 gcatcattat aatgaatgct ggtaccatat taatgcagta tacaaaaaag ctagaaatag
     9181 atgctttaac caaatcttct gcgccctgat tagttaaagc gaatttgttt ttataaaaac
     9241 ttttcattgt caagcctcca atcatttgca ttctcataat tagtgaccaa ttgtttatac
     9301 acttgatgtt tagccattaa ttcattatga tttccacgtt ctactatttt cccttctgac
     9361 aagacaagaa tttcatctac agatttaatg ctggataatc gatgagctat cataataact
     9421 gttttttctt tcatcagatg ttgaaaagcc ttttgtagtt catactcatt atctggatca
     9481 atagatgcac tggcttcatc cataataaca atctttgcat cttttaaaat tgctctggca
     9541 atagctatac gttgtttttc tcctcctgac agataaactc ctttactacc aattaaggta
     9601 tgctctcttt cgggaaattt ttctaaaatc gattcgcaac cggctaatct tagcgcgtca
     9661 aatactttac ttgctggcgc ttgaggattt gctatagaaa cgttatcgta aatactctga
     9721 ttgaatagtt tcgtatcttg aaagacaaaa gcaattgtgt taataattgt gtcttttgaa
     9781 tagtgattta aaggcttacc accaatttta atctgtccct tatccacatt gtagaaacct
     9841 gaaattagtt tagctatcgt actctttccg cttcctgatt ttcctactaa tgcataaatt
     9901 cttttttgtt ctaaaactaa gttgaaattt tctaaaacac actcttcttt gtatgaaaag
     9961 ctgacatttt caaatgcaat gtcacaattt ttaatctgtg tctcgttccc atattttagt
    10021 tggtcttgct tcatttctaa ataaagggag tctagtgttt cgactgcata tcttgcttca
    10081 aacaagtgca tacttacata cattattctc atgaaagaaa caaaaataat tccacttaaa
    10141 aaaaacaaca tcaacaagtc aacagctaat atcttgggag tggtaagaga agttataaaa
    10201 aaggttatag gaactatcaa aatgcaaact atgccaaaaa atagccattg aaaaagaaca
    10261 aaaggtgttt tcccactcaa agaatactta tatgcaaact tagcataatc gttaattgct
    10321 ttattcaagg ctttaaatga tgttacttta gcaccaaata tctttataac ttgaattcct
    10381 cggatatatt ctaccgtttc agagcttaac acatctaacg attcttgata tttttgaata
    10441 aaatttgtat tctctgtcat tttctttaac aagaatacac caattactaa catggcgatt
    10501 agaaccaatc caattttaaa attaatgaaa aagcctaagg caactgataa aacaggtgtt
    10561 actatggctt gagcactatc tggaatcaag tgagcaattg ccatatgtgt tttagccgcg
    10621 ttgtcatcaa ttgtctttcg aatatatccc gaagaattta aatcaaaaaa acgaaaactt
    10681 gcttctgtta aaccgctaat tccctttttt ctcaaattag tctctaagcg aaacccaagg
    10741 atatgagaaa ataagccaga aaaaatataa aataaagctc ctaaagttaa ccaagcaaca
    10801 gtttgaaccg cataactctt agctaaatat tcatctccta caataattag agacttaaga
    10861 aatttgtaaa tactgtaata tccatagact gttaatacga cagaaaaatc agaaaataaa
    10921 gtggctaacc atccgtagta tttcatttta gggacataac taaataattt tttgtatact
    10981 ttcatttttt cctcccccgc attcaatttc ttctcttatt cgtttacaac tgttaatgct
    11041 ttccgtaata gcgtaaatta gagatgtaca aacagataga aggtaaacta cgttctattt
    11101 tttgtatttt tttaagactt ttcttccaat caatacacca atcatagata aacttgctac
    11161 aactaacact gaaatgattc cccatatacc taccgcatag tggatcattg catccattgt
    11221 tgcagcagat tgtcctttat ctatccaatc ttgacgaaaa cgatctgtaa agaaccatat
    11281 aggaatccac tgtccggcag cactgcctaa ttgcattaga gcccaagacg caacttgagg
    11341 aattgctcga tcatatccaa atacagatag aataatatca gctaagatcg ccatactaat
    11401 cgaactgata aaccaaggta agtacgcccc gcccattaac gtaaaaaata acaataaaac
    11461 aattgtgtaa atagtaaaaa ttccttttct agggtagtta tagctaatat aaacaaatat
    11521 gattccagat aataatccaa aaatccctgg ggatatgctg tgaccaaacg ccccaccaat
    11581 actactaacc attattgaaa aaaagtaaat tactagggaa ataactgtag ctaaaccaat
    11641 aaaaatataa tcttttgttt tcatatatat tctcctcaat tctatttcgt tatgtcatta
    11701 aaaatctgtt ctatcaaagt tgaatcttct actctaagtt ttatatcttt ttttattcca
    11761 ccatttttta aataaataat tctattgcat gttttataaa gaaattccgg atcatgagtg
    11821 gctataacta ctgcaccctt ttcttttaag tctttaataa ggtcagcaat cctttccata
    11881 ttaatataat ccaacccact tgtaggttca tcaaatagaa ataaattagt ttttgacaat
    11941 gaagctatag aaaccaataa tctttgtttt tctccaccgg ataaatcaaa cggtaattga
    12001 tctttcattt tataaagttc catttttttt aaaatttttt cttgctgatc tttactaact
    12061 atttcattcc ccagggctaa ctcgctcctc actgattctg tgaaaaattg gtaatccatt
    12121 tcttgcatta ctaaaaacgg cgatgacaaa ttttttttat tttcgattat ctttcctttg
    12181 gtagggcgaa taatcttcat gagtattctt agtaaggttg ttttccccac accgtttttc
    12241 cctaccaaac caataacatc tccaactttg acatcaaaat ttatgtcttt tagaatattt
    12301 tcataagtaa tttcttcaac ttttaataca tctgcactta ccttttcgat atagggaact
    12361 tcaatcgaac tattaaaaat gtcaaatttc cttaatgcgt tattgttttc agtttttaac
    12421 aactgaatag ttgtagtttt tacagttcta tttttaacaa atagaacttg atcaattaat
    12481 tctttcaaat agtaaaaacg atgttcagct ataacaatag taaatccttt attttttaaa
    12541 tcacaaagta agtcagcaag tttcttagtc gctttgaagt ccaaatttga agacggttca
    12601 tccaatataa ttaatttaga gtttagcgat aaactacatc caatagaaat tttctgccgt
    12661 tcaccacttg atagcgaaaa aatgttttta tctaatatat ttgaaagatc taataattca
    12721 gctaattcat tgattctttt atccatatgc tcttttgtaa atccaaagtt ttccatcgaa
    12781 aatactaact cactcgttgt atttgttgta aagaattgtc ctctaggatc ttgacttacg
    12841 tagccaatat tttttgatat actttcttgt gttaaggatt gtagcgagtt attttcaaat
    12901 aaaatttccc caaccaattt tccgtcataa agtcttggaa taagtccatt cattaacttt
    12961 aatagtgtgg atttcccact cccagaagaa ccagtaatca atgtaagact accttttttt
    13021 attgaaaaat taaggtcaac aagcgcttgt tccttaacat ccttatatga aaaagaaaca
    13081 ttgttaaatt caaacaattc tttcacctca aatcaaaata cttaagaaaa aaatagtaac
    13141 ggaaaacagg atgatatagt ctaataagct catgtttgtt ttatagtaag aacttctttt
    13201 cacaggagat tctatcccct ttgttattgc attagcggtc atttcttcag caatcatagt
    13261 tgcacgtata agaacgggaa ctaacagaaa ttccaaatat tgcagaggct gaaaaaaaga
    13321 caagcgaatg tttctcattt ttaaagattc tttaatcatt cttatttcag aacgataagt
    13381 cggaaagaat ctgatcacta ctgtaatact taacattatt tttttcggta aatgtatttt
    13441 ttctaaagag gataataatt cattgctagt atagacacta accaacaacc atgagatcat
    13501 tacgaccggc aacattcgaa atacaatata taaggatgtc ccaaaaaata tagtaaaaaa
    13561 ctctatcttg atagcatcaa taattttcat tactaaaaac agaactgaat aaatcaagat
    13621 gaaataaata catgtcttta ttttacgaaa catcaatact aaaactgtag caataatgaa
    13681 acaccccact tgaaatatat ttccctttcc taaaaaaata atgaccggaa aaaaaatatt
    13741 taaagtaatc aaggttaacg gattgatttt tgacactctt tcacctccaa aaaataagat
    13801 cgcactgtta tcttaagctg ataaaaatgc accgttattc agataacagt gcgatcttat
    13861 caaaaagcat aactgagaac aattctcaat gcaagctttt ttttaattaa ctcagtaatt
    13921 caatccaccc aggaacagaa aattgataca atcttctggt taatagcata gtcttttctt
    13981 ttgaatagct atgcttcaat atatcataga atgtagcgta gaatgagtaa atgtagaagt
    14041 ggatttcttc ttcattaaag actaaatttt gattgatcat tccgatagtc tttaacttat
    14101 ctagacaaca gactgtcttt tttgtcacaa atgatgtaat tttttcaaca atatcttgat
    14161 atttggttcc agctgattgg aataaaacca aatcgaatat acgtttattc tcaaaaatat
    14221 aattaataaa tggatctaat tcattgaagt tctctctcca acttttctct atatctatat
    14281 tttctaagtt taagaggtat ttggtaaact cttcattata catattaata agcgcattaa
    14341 tatgcggctg aataatttct tcaaatagcg cttccttaga agtaaaatgt ttataaaaag
    14401 cacccgtagt tacgcctgcg gtcaagcaca gttgccttat cttagtttgc tgaaatccta
    14461 ctttttcaaa atggcttagt ccactttcta ttaatttttg gtgagttgta atataatctc
    14521 tggtttctat gggtacttcc tcctaataag atgaattaac tatattatct tacacaggac
    14581 ttgaagtttc aatcgttaac ctctattact gaactcattc cttagagcta aactaatgag
    14641 aaagagcaaa ataactgata caaaaagcaa tcctacaaga attaatagac tcatctaaat
    14701 cattcacctt ttctaaaata atccgagaaa gatttaaaaa aattatatcc agctattagg
    14761 ataagtccta tcataatcac tccaatagcc attttcttat cctccctaaa aataaagcct
    14821 tttacttaaa accttttttg taatcgtcaa taaatttttt ccctttatca gttaattgaa
    14881 atcctatatc taaatagtcc tcatatctaa gtttagtcag catctcactt acagatgctg
    14941 gactaacact tagaactttt gctaaatcat tattttttaa tttctttttt ctttttgata
    15001 attcctgaat tgctagtaaa taaattagta tagtagttgt tttaatttgt accataccta
    15061 ccctccaaat tatttcgaaa ggccttcagc aattttatcc aggttccatt tcatcatcgc
    15121 atagtaacta tcaccatctt gtcctttttc tgcaattgaa tcagtaaaaa tcgttgaata
    15181 gataggaata ttggtatctt ttgatactgt tttcattggt ctatcgtcca cactgctttc
    15241 tacgaataag gagggaactt ttgttgtgcg taatttttca actaagtgtt ttatttgatc
    15301 tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa gcagaaggca cattatacgc
    15361 tttcgagaaa tatttaaagc atccttcact tgtaacaatc atttttttat cattcggaat
    15421 tgaagcaaat ttagatttag cttctttgtc tagagaatcc aatttttcaa tatacttatc
    15481 tagattttct ttatagaatt ttttattatc aggatctttt tccgctaact gtttttcaat
    15541 atttttagcg taaataatac cgttttctaa atttaaccaa gcatggggat cttccttccc
    15601 tttctcactc tgaccctcta agtaaataac atctatgcca tcacttgctg caaaatagtc
    15661 tttgttttcc tctttgttcg cattttttac taattttgta aaccaagcat ttcctccagt
    15721 ttccaagtta acaccgttat aaaaaatcaa atctgctttt gaagtttttt gaacatcttc
    15781 aggcaaaggt tcatattcgt ggggatcttt cccaataggt acgatactgt gtaaatcaat
    15841 tttatctttt gctatatttt cagtaatatc tgctaaaatc gaattagtaa ctactacctt
    15901 taatttttcg ttcgaagcca ctgtatcttt gtctttacta tttgttgaac atgccccgaa
    15961 tataaaaaga gcaatcaaaa tccccagact tcccagtact gctttccatt ttctcatcat
    16021 aactttctct ccttctttct taaaaatttt tgttttggtg agattagaaa actaattaca
    16081 aataagaatg ccgctgttaa aacaatactt gatcctgctg ctatgttaaa actataacca
    16141 atgaacaaac ctagcaatga tgagcacgct ccgactgtcg ccgaaataaa tatcatagtt
    16201 tttaaactgt ttgcatataa atatgctgta gcagctggtg taattaacat tgcaacaatt
    16261 aatatagttc ccacactttg catagctgtt actgctacta aagttaatag aatcattaat
    16321 agataatgat aaaaactgac attcatcccc attgcttttg ccattaatgg atcaaaagaa
    16381 gtgattaata attctttaaa aaatagaaca actgtaacca acactaaaac agaaacgcca
    16441 attgtaatcc acttatcaat atcttgtaca gctaaaatat ttccaaataa gatatgaaat
    16501 aaatccgttg agctatttgc aactcctata aggataactc ctaatgctaa aaaagaactg
    16561 aaagtaatac caattgctgt atctcctttt attacactat tgctttttat aaaagtaata
    16621 atgatagaag ctaatagacc aaatacaatc gcaccaataa aaaaattaat acctaaaata
    16681 taagaaattg ctactcctgg caaaacagca tgagaaatag catcacccat aagagacatt
    16741 cctcttaaaa taataaaaca acctactgtt ccagaaacaa tccctataac tacagatgta
    16801 attagagcat tctgtaaaaa gtgaaagtca tacagaccat taataaaatt ttgaagcata
    16861 ttattcccct tgtatattag agttagggtt ttaaccttcc ggttttgatt ggataaccct
    16921 atgaaccatc gttattttaa tttggtatta actcatttct tttgagataa tatatttatt
    16981 ggattgagag gctaagccgt tcccccctct ccaataaata tagtatctcc ataggctttt
    17041 tttaaattct cttcattaaa tacagattct acacttccgt gaacaattag atttcgattc
    17101 aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc
    17161 actgtttttc cctgatgttt caattctttt aacgtatcca taattattcg ttcactaact
    17221 aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat
    17281 cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa
    17341 tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta
    17401 attctttgaa aaactttcat tccggcatat gttcccatag atacacattc ttttactttt
    17461 attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt
    17521 ttattgatat cttcattatt aatggagacc tctccctgat gagggataat atttaacatt
    17581 gctttaagca atgtagattt tccagcacca tttggaccaa taatacctgt taaagcacct
    17641 tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc
    17701 tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg
    17761 tatacaaaaa ttatatttta gtcaacccta aattttataa tttagatttt tttctgttgt
    17821 tgaagattag gatgtcaaaa ttatttgaaa ctactcgtat agtaaatagt aagtaatttt
    17881 caaaacacga agaaaaacaa aaagaaaaaa ggaaacgccg aagcatttcc tttcagtgat
    17941 ttaatgagaa caacacgatt tgacttagag tctcaaaaag tcgaataggt acactcattt
    18001 aagtgtctcc attcgacttt ttggggatga gctggctatt gaggtccgct taccctattg
    18061 aataaataca agattgggta tttctaataa gtccgccctt catattgggg tgatcggggc
    18121 gaacatgcca tcttttcata taccacctcc tcgacactca ataatcaaag attctcgtta
    18181 atcttcttcg atgcttgcaa tgatgttttc aatattcata taacgaaaat cttcttgttt
    18241 gatataaacg atctgagcgg tacatttttc ctttttgatg atttctttta ctttttcctc
    18301 agaacgtgcg acctgcggcg ttaaaagtaa aacgtcatag tctttataat tcagtttttc
    18361 ttcaacgttt cggatcgttg cataatctat ctcatacgat agatttttgg ctctgatcgt
    18421 ttcaagcatt tttgatacga gcagcgacgc actgattccg gtctcgcaaa taatcaatag
    18481 atccttatgc ataataccct cctgttttta gcctgcaaag ccaattggtg aaaatgccac
    18541 ataagttaaa ataacaatga ctaccaatgc tatagcagtc accttggtgt atttccaagg
    18601 ctcgatatcg acctttgtat cgaatgagtt caacacaaat tctccatcag gtttcaattt
    18661 ggaaaccacc atcaaaatga taatgtccag aacgaataat gccgtcaaaa cataaaggaa
    18721 atggatattt tggaaaagca tttttgatag tgcatacaag acgatatgca cggcaatcgt
    18781 cgtttttgcc ccaaaagcag ttgctttttt tgaataaaag gcaaagagaa tgatcacgag
    18841 cagcggcatg ttgtatagtc cattgaattc ttgaacgaac tgatacaatc cagccggtgc
    18901 aaaagaaatg aatggcgcta cgataatgga gataatcccc acaacgaccg ttactgtttt
    18961 accagcacga gcgatttgct tatcatttgc tttcttattg ataagcggtt tataaaaatc
    19021 cagtgaaaac aaggtcgatg tcgcgttcaa agctcccgca aatgatgata agatcgcgcc
    19081 aaagataaca gcggcgaaaa taccaaagat cattttaggc aatacttcac tgactaaaaa
    19141 aggatacgcg ttatctggat ttgacattaa actatcgcca aaaatattgc gagcgacgat
    19201 ccctggaaag acaaggaata acgcacctaa gattttgaag acgccgacta ggaaagcgcc
    19261 cttttgtgcc tcagctaaat tcttgccgga caatgctttt tggacgatca tttgattggt
    19321 acaccagaaa tacagattgt taaacagcat ccctgtaaat agtgtcggcc atggaacaac
    19381 agccgagtcg atcgatccca cagagttaag cagccatggc gtgtttacca caacgtgttc
    19441 gatcccgcca acaaagctgc cgtcaccaag cttcattaag cctaaaattg ggaccagcag
    19501 accacaagcc agcagaccga ctccataaat ggagtcacta ttggcactta acgacaagcc
    19561 gccgatcagc agatagagga tgccgacaat accaattgcc atcgctacaa ttatgatcgc
    19621 gacgatcggc tggacgccca gcagctcatc aatatggaag atcttattga acaccagcga
    19681 tccagaataa agaaccacgg gtaagaaaga agtcatatag gtcacaataa ataagatcga
    19741 aataattcgt ttggtaaccg tatcataacg aatctcgatg aaatctgaga ttgtattgat
    19801 gccgtattta aaatattttg gcaagaatat cacagccaaa gctacgatcg caatcgtagc
    19861 agttacttcc caggccatta cttccatccc tgcatgataa ctttgaccat tttgtccgac
    19921 tatttgttcg gtggacaggt tcgtcatgat gatcgtgccg gcgattggca aagcagttaa
    19981 gctccgccct cccatgaaga acccttctga tccagaagta tcgacacttc tggatctaga
    20041 ataggcaaat aaccatacac agatgacaat cactaggaaa ctgattatag aaaaaagatt
    20101 catcgtctag ctccttttca atagattaaa gaccggtgac ctctcgaatg tagcttctgg
    20161 ctttgactgc atattcaaat ggattggctt tagccgggtc ctgttctgct tcaactacga
    20221 tccagccttc atagtcgctg gcatggattg ctgtccagat tggagtgaaa tcaatcatcc
    20281 catcaccagg aaccgtgaag acgcctttct tcacgccgtt taagaagctg tctttttcct
    20341 tttctacttc ttcagccgtt tcttgacgaa tatctttgaa atggatgtgt ttgatacgat
    20401 cttgatgacg gttgtaaacc gcgattggat tttcaccaga aaagaccaaa tgtcccgtat
    20461 cgaacaataa tttaactttg tctggatcag tcatttccat taaacgatcg atttcttccg
    20521 tcgtttgaac gccggtaccc atgtgatgat ggtaaacgat ctccatatcc ttttcgtggg
    20581 ccaaatcccc taatttttct aatccttccg ccagtttttt ccactcttca tcagtgaaaa
    20641 ctggtttatc atcaaagact ggcgctgtca taatcccttg gatactatga ccctgctcag
    20701 ctactacgat gactttagcg cccatcgcat gtaagaaatc ccgatgtgct ttgaaggctt
    20761 cggcagtttc ttcaaaaggt ttagttgtca gaaacgcact gaaccatgca ctggcaacct
    20821 ttaagccgcg aatattcaaa tattgttcca acacctctgg atcttttgga tatttgttgc
    20881 caatctctgt tccggtgaac ccagcaaggg ccatttcact gatacattgt tcaaaggtat
    20941 tttcactacc aagctcgggc atatcatcat tggtccacgc gattggtgcg atggctaatt
    21001 gaatttgttt ggtcatattc tcacactcct gcttattttt ttagttgtac taaactcttc
    21061 gtatcgaatg attcttgaca agccaacgcg atcttagttg attgcaatcc atcttctgca
    21121 ttaaccgctg gttgacgttt ttcttgaata catgagacaa attcttgggc ttcacgaatg
    21181 aaggcttctc tgaatcgttc tgggaagttt tgtgaacatg gacggatgat cccttgatcg
    21241 ttcatgatcg tcacaagatt tttctcaggc gcttgcgcta cccgcagcat tcctttggtt
    21301 cccatgatct ctgtttcgac atgatagcca tgggctgcgt ttctgccggc aactagtaaa
    21361 gctattgttt tatcgctcag gctcatcatt gcggctccag tttcaagctc accagcctga
    21421 tccaattctg gatacgccgc atttttccca atcgcccaca cttgatcgac ttcttggccg
    21481 gtaaaccagc gaaccaaatc aatatcatgg atcgccatat ctaagaacaa gccgccgctg
    21541 tcgttattca tggcaaattt gacaaagctt tccaagcctt cgctcggatc gatcccataa
    21601 caacgaatca gtgtgatttc gccgatttcg ccatcttcta ccattttctt cgcatactga
    21661 tacgactcat catagcggcg catgaagcct aacatgaaga cctgttcagg atgctcttcg
    21721 atgacttttt gggtcttttt aatatcttca acttcaaggc ctaatggttt ttcagtaaag
    21781 acatgcttgc cattttccat cgccaagcgg acttggtcac agtgaaagcc tgatggtgaa
    21841 acaataaaga ctgcatcgat cacggaactt ttgaccattt cttcatagct ggaaaaagta
    21901 tgcttgacgc ctaatttctt ctgtgcgaat gctaattctg tttcaactaa actgcacgca
    21961 gccgtaagct cacaacaact gacacctgtt gctaaatttt gtgcatgtac ttttcctaat
    22021 cttcctaaac cgacgatgcc tactctaatg ttgttcatga aatacctccg ttttctgaga
    22081 attactcatt gtatagtgct ggtcgttcat ccatttgaat cattactttt gtttttgttt
    22141 cacgagactc atgacaagcc atcatggtga tcgctgctac atacccatcc caagcggtag
    22201 gaccgacaac cgcatcattt ttgatcgaat cgatccacag cttgaattca taatcatagg
    22261 catccaaaaa gcgggtctgc caatctggtg aaacaggtgt gtaatttttt tcttccgatt
    22321 taattcgtgt ataggctggg tccgtcattc ctacttctgc cgtttcgaaa accacatcac
    22381 atttaatatc gtagccaaag cggcaattca caaagatttc caaatcaatc gttacaccgg
    22441 attttgtttg aaagatcagc agctgcggat catgcaaatc ggcatgggag ttgctggttt
    22501 ttttcggcaa gatcatttga acagattcat aatcttcatt tagcaaccag cgaagcacat
    22561 caatctcatg aatcgcggtg ttgctgatcg ccatcggtgt tgtgtagttt tcatctgcgc
    22621 taggatttct atgggcacaa tgcatcatta atgcttctcc gtattgcttc gattcgattg
    22681 cttctttcag ttcaatgtag ccgcggtcat agcggcgcat gaagccaacc tgaaccaatt
    22741 tcttcccata agcaacttcc gcatcaacga tccggcggca gcctgctgca tccatcgcta
    22801 atggcttttc gcagaaaaca tatttcccat gcttgattgc ttctaacaca tactgctcat
    22861 gggtcgggtc ccaagaagtc acaacaacga catccacatc ttcagaagcg atcaatgctt
    22921 ctccggtttc aaagaattta gcatcgacac gtttggcaac ctctttagca gcttttacat
    22981 tgatatctga gactgctgtc acataagccc cttgtgtttt ctgattgatc ctgtcgatat
    23041 gctcgatgcc gattgcacct gttccgataa cgcctacttt tactctcaat tacttatccc
    23101 cctgttctgt tgtttaagca cacatagtat gcgcttacaa aattattcct gtgtagcttt
    23161 tattcttaaa aggtagagcc ctctctttgc tccgcctttt actacccata agcttgtacg
    23221 tataattcct taatttgact atgactaggc acctttttat tattttgcgg acttccgctg
    23281 gtcaacgcat ccgcggccat tttgtctaag gctgccataa acgcttgctg atcaatacca
    23341 taatctcgaa tcgttgggat cgacagctgc tcacagagct cttggatttt tttaatcaga
    23401 taatccgcag cctcttccag cgataaatct tcggctgccg gataaataaa tctggcgatc
    23461 gtcgctaact cagtctgaat agcatccttt gagaattcaa aaaagaccgg caataacatc
    23521 gcatttgata tcccatgagg aatgtgaaaa agtgccccca ccggtcgact cattccatga
    23581 atcaatgtga ctgacgcatt cgaaaaggca atccccgcct ccgttgaagc taagctcatt
    23641 tgttctctgg cttcagtgtc gtcgctattt tcatatgcct gagaaagcgt gttcaaaatt
    23701 ttcttcatcg cttccagtga aaatagttgc gttacttcat tggctttgac tgaaacataa
    23761 gattccaaag cgtgacatag cgcatcgact cccgttgccg cggttacttt tggtggtaca
    23821 gttttcgtaa aatcagagtc aacgattgag atctgtggca tgaaaacgag atccttcatc
    23881 atcattttga ctgctgtttt tgtattggta ataactgcca catcggttac ttccgaacca
    23941 gttccagcag ttgttgacac tgcaatcagc ggaatgcccg cttgcagctg atcaaaattt
    24001 cttgcatgaa tcgcttcgag acttaattga ttagcagcca aaacggctac caccttggct
    24061 gtatccagac agctgccgcc accaaaagca atgatatgat cacattgatg cttctgaaaa
    24121 agcttcagcg catcataaac attttcttgc gtcggttcgg cttgaatgtc tgtatactcc
    24181 gctaattgat catctgtttt tcccaacagc tccttgatct tttgaagatg cccggtctgt
    24241 tccataaccg tatcactaat gattaacggc ttgttgccta gttgttctga ttcttccata
    24301 agaaattgga ttgttccgcc gccaattctt aaattggcag gcattttcag ttgaatattc
    24361 acagaaaaaa cctctctctg gcttatagcc tataacagaa tcctcgaagc tctttaacag
    24421 agcttcgaag caaatcttta aatctcactt tgcgcataac gtgctgtaac aactttccga
    24481 tgggtataaa actcaacact gtctttgcca ttggcatgca aatcaccaaa gaaagagtgc
    24541 ttccagcctg aaaaggcaaa ccaagccatc ggtgcaggca ccccaagatt gacccccagc
    24601 atcccagcat caacattttc tctgaagtag cgaatcgctg atgcgttgtt ggtatacaga
    24661 cacgaaccat tcgcaaattc agattcattc gtcagctcaa cggctgccat caaatctttt
    24721 acccgcacga ttgatgcgac cggggcaaaa atctcatcct gccagatctt catatccatc
    24781 gtgacattat caaaaatcgt tggtccaaca aaatagcctt tttctggaat gttttcgcga
    24841 ccatctagta ccagtttcgc tccttcatcg accccttttt ggatatagtc aaaggtccgc
    24901 tgctgtgcat cttttcgaat cactggtcct aaaaagaccc cttcgtccaa gccattgccg
    24961 atcttgatcg cttttgccgc agccttaaat ttttcaacaa actgatccgc gatttcctct
    25021 tccaaaacaa tgacggaggc tgccatgcag cgttcaccag cagaaccaaa agctccgcca
    25081 ataaggtcct tgactgcccg atcaatattg gcatctttca gcacaacaac atgatttttc
    25141 gctccagtca acgcttgtac ccgttttaga ttttttgttc cttcacggta aacgtattca
    25201 ccgacatttt ttgatcctac aaagctgatg ccttttacca gtggatcttg cagcaatgtg
    25261 ttgacaacat cgtgggcacc atagacaaca ttaaaaacac cgctaggaaa gcctgctttt
    25321 tcaaccagct caacaatttt ctccatcaac aatggtgttt tttctgaagg cttcagcacc
    25381 accgtatttc ctgccgccag agccatcggg aagatccaga agggcaccat cattggaaaa
    25441 ttaaacggtg tgatccctcc gattactccg attgggtaac gataattcgt gatctcaata
    25501 tcagtcgccg ctgttgataa agaatctccc atcattaaat tggtgattcc cgctgcgtgc
    25561 tccacatttt cgattcctcg ttgaacttct cccagtgact cggacaatga tttcccattt
    25621 tccagcgtga tcaaacgcgc caattcactt ttatgatctg ttaaaagctg atgcagcttg
    25681 aacagataac gtccgcgaac aggaacagga acttttttcc aagtttgaaa cgcagcatcg
    25741 gcagctttaa ctgcttcggc tgtctctgct ttgctagaca acggtacatt cgccagcgct
    25801 tcttctgtag ccggattcat tacttcttca aactctgttg agcgactagc gacccaacga
    25861 ccatcaatat aatttcttaa aaatcttgtc attta
//
LOCUS       DACCKU010000035        25535 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.037, whole genome shotgun sequence.
ACCESSION   DACCKU010000035 DACCKU010000000
VERSION     DACCKU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.037"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVN13_001376"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IVN13_001376"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7011872.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IVN13_001377"
     CDS             complement(937..1518)
                     /locus_tag="IVN13_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7011873.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVN13_001378"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IVN13_001378"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP7011874.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKGFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IVN13_001379"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IVN13_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP7011875.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IVN13_001380"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IVN13_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP7011876.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IVN13_001381"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IVN13_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7011877.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IVN13_001382"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IVN13_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7011878.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7566..7874
                     /gene="rpsJ"
                     /locus_tag="IVN13_001383"
     CDS             7566..7874
                     /gene="rpsJ"
                     /locus_tag="IVN13_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP7011879.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7905..8534
                     /gene="rplC"
                     /locus_tag="IVN13_001384"
     CDS             7905..8534
                     /gene="rplC"
                     /locus_tag="IVN13_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP7011880.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8562..9185
                     /gene="rplD"
                     /locus_tag="IVN13_001385"
     CDS             8562..9185
                     /gene="rplD"
                     /locus_tag="IVN13_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP7011881.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRLPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9185..9475
                     /gene="rplW"
                     /locus_tag="IVN13_001386"
     CDS             9185..9475
                     /gene="rplW"
                     /locus_tag="IVN13_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP7011882.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9514..10347
                     /gene="rplB"
                     /locus_tag="IVN13_001387"
     CDS             9514..10347
                     /gene="rplB"
                     /locus_tag="IVN13_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP7011883.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10390..10668
                     /gene="rpsS"
                     /locus_tag="IVN13_001388"
     CDS             10390..10668
                     /gene="rpsS"
                     /locus_tag="IVN13_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP7011884.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10691..11038
                     /gene="rplV"
                     /locus_tag="IVN13_001389"
     CDS             10691..11038
                     /gene="rplV"
                     /locus_tag="IVN13_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP7011885.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11052..11708
                     /gene="rpsC"
                     /locus_tag="IVN13_001390"
     CDS             11052..11708
                     /gene="rpsC"
                     /locus_tag="IVN13_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP7011886.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11711..12145
                     /gene="rplP"
                     /locus_tag="IVN13_001391"
     CDS             11711..12145
                     /gene="rplP"
                     /locus_tag="IVN13_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP7011887.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12135..12323
                     /gene="rpmC"
                     /locus_tag="IVN13_001392"
     CDS             12135..12323
                     /gene="rpmC"
                     /locus_tag="IVN13_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP7011888.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12348..12614
                     /gene="rpsQ"
                     /locus_tag="IVN13_001393"
     CDS             12348..12614
                     /gene="rpsQ"
                     /locus_tag="IVN13_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP7011889.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12672..13040
                     /gene="rplN"
                     /locus_tag="IVN13_001394"
     CDS             12672..13040
                     /gene="rplN"
                     /locus_tag="IVN13_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP7011890.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13077..13385
                     /gene="rplX"
                     /locus_tag="IVN13_001395"
     CDS             13077..13385
                     /gene="rplX"
                     /locus_tag="IVN13_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP7011891.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13413..13952
                     /gene="rplE"
                     /locus_tag="IVN13_001396"
     CDS             13413..13952
                     /gene="rplE"
                     /locus_tag="IVN13_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP7011892.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13971..14156
                     /locus_tag="IVN13_001397"
     CDS             13971..14156
                     /locus_tag="IVN13_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP7011893.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14192..14590
                     /gene="rpsH"
                     /locus_tag="IVN13_001398"
     CDS             14192..14590
                     /gene="rpsH"
                     /locus_tag="IVN13_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP7011894.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14622..15158
                     /gene="rplF"
                     /locus_tag="IVN13_001399"
     CDS             14622..15158
                     /gene="rplF"
                     /locus_tag="IVN13_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP7011895.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15319..15675
                     /gene="rplR"
                     /locus_tag="IVN13_001400"
     CDS             15319..15675
                     /gene="rplR"
                     /locus_tag="IVN13_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP7011896.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAKAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15696..16196
                     /gene="rpsE"
                     /locus_tag="IVN13_001401"
     CDS             15696..16196
                     /gene="rpsE"
                     /locus_tag="IVN13_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP7011897.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16211..16390
                     /gene="rpmD"
                     /locus_tag="IVN13_001402"
     CDS             16211..16390
                     /gene="rpmD"
                     /locus_tag="IVN13_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP7011898.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16434..16874
                     /gene="rplO"
                     /locus_tag="IVN13_001403"
     CDS             16434..16874
                     /gene="rplO"
                     /locus_tag="IVN13_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP7011899.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16874..18169
                     /gene="secY"
                     /locus_tag="IVN13_001404"
     CDS             16874..18169
                     /gene="secY"
                     /locus_tag="IVN13_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP7011900.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18231..18878
                     /locus_tag="IVN13_001405"
     CDS             18231..18878
                     /locus_tag="IVN13_001405"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP7011901.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19069..19287
                     /gene="infA"
                     /locus_tag="IVN13_001406"
     CDS             19069..19287
                     /gene="infA"
                     /locus_tag="IVN13_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP7011902.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19320..19436
                     /gene="rpmJ"
                     /locus_tag="IVN13_001407"
     CDS             19320..19436
                     /gene="rpmJ"
                     /locus_tag="IVN13_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP7011903.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19454..19819
                     /gene="rpsM"
                     /locus_tag="IVN13_001408"
     CDS             19454..19819
                     /gene="rpsM"
                     /locus_tag="IVN13_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP7011904.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILANAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19847..20236
                     /gene="rpsK"
                     /locus_tag="IVN13_001409"
     CDS             19847..20236
                     /gene="rpsK"
                     /locus_tag="IVN13_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP7011905.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20316..21254
                     /locus_tag="IVN13_001410"
     CDS             20316..21254
                     /locus_tag="IVN13_001410"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP7011906.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21282..21662
                     /gene="rplQ"
                     /locus_tag="IVN13_001411"
     CDS             21282..21662
                     /gene="rplQ"
                     /locus_tag="IVN13_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP7011907.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21934..22773
                     /locus_tag="IVN13_001412"
     CDS             21934..22773
                     /locus_tag="IVN13_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7011908.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22749..23618
                     /locus_tag="IVN13_001413"
     CDS             22749..23618
                     /locus_tag="IVN13_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7011909.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23608..24405
                     /locus_tag="IVN13_001414"
     CDS             23608..24405
                     /locus_tag="IVN13_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP7011910.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24608..25354
                     /gene="truA"
                     /locus_tag="IVN13_001415"
     CDS             24608..25354
                     /gene="truA"
                     /locus_tag="IVN13_001415"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7011911.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
BASE COUNT         8299 a         4712 c         5495 g         7029 t
ORIGIN      
        1 agttggcagg agaacaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgtactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catatttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aggacttctc cgttttcgtc
     1861 tgtacgcaag acaggttgga atcctttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatcccttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat tatggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact acactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg atcgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attacttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt ctcaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataatag aaggaacgcg aaataaaaga gtcagtattt
     7321 tctaaatgaa actttatgaa aacaaaatgt tttttacaag aaaaatcctt gctttgtgag
     7381 tttatatata ttataatgac aaaggtgcgt ttactttagt gaattgcaca gacgtaatca
     7441 gaatgatttc gtcggctaag aaacgagagg ttgcgacacg cccggacgct ttgccacgaa
     7501 cgagcgtgac ggaaattttc gtggagctat gtctactttt caaaataggc gaaggaggga
     7561 acaagatggc aaaacaaaaa attcgtatcc gtttaaaagc gtatgaacac cgtattttag
     7621 atcaatcagc ggataaaatc gtggaaactg caaaaagaac tggagctagc gtgtctggtc
     7681 caattccatt accaactgaa cgcagtcttt acacaatcat ccgtgcgact cataaataca
     7741 aagattcacg cgaacaattc gaaatgcgta cacacaaacg tcttatcgac attgtgaacc
     7801 caacaccaaa aactgttgat gctttgatga agctagactt accatctggt gtaaacatcg
     7861 aaatcaaatt ataaaactaa aacaaaaata atggaggtgt actcatgacc aaaggaatct
     7921 tagggaaaaa agtgggaatg acacaaatct tcactgaatc tggtgaatta attccagtaa
     7981 ctgttgtaga agctactcca aacgtagtac tacaagtgaa aacaatggaa acggatggct
     8041 acgaagctat tcaagttggt taccaagata tgcgtgaagt tttatcaaac aaacctgcga
     8101 aaggtcatgt tgcaaaagca aacacggctc ctaagcgctt cattcgtgaa ttcaaaaatg
     8161 ttgagctagg agaatatgaa gtaggaaaag aaattacagt agatgttttc caagcaggag
     8221 acattattga tgtaacaggt actacgaaag gtaaaggatt ccaaggggtt atcaaacgcc
     8281 acggacaaag ccgcggacca atggctcacg gttctcgtta ccatcgtcgt cctgggtcaa
     8341 tgggtccagt tgcacctaac cgtgtattta aaaacaaacg tcttgctggc cgtatgggtg
     8401 gcaaccgcgt aactatccaa aacttggaag ttgtacgtgt cgatgcagaa agaaatgtta
     8461 tcttgatcaa aggtaatgtt cctggagcta aaaaatcttt aatcactatc aaatcagctg
     8521 tgaaagctaa ataattgtag gaagaaagga ggaactaagg aatgccgaat gtagcattat
     8581 tcaaacaaga tggaagccaa aacggtgaaa tcacacttaa cgaagaaatc ttcggaattg
     8641 aacctaatga aagtgttgtt tacgatgcaa tcgtaatgca acgtgcgtca ttaagacaag
     8701 gaacacacgc ggttaaaaac cgtagcgctg ttcgtggcgg tggacgtaaa ccatggcgtc
     8761 aaaaaggaac aggtcgtgca cgtcaaggat ctatccgctt accacaatgg cgtggaggtg
     8821 gcgtagtctt tggaccaaca ccacgttctt acagctacaa acttcctaaa aaagttcgtc
     8881 gtttagcaat gaaatctgta ttgtcagaaa aagttgctga aaacaacttg gtagcaatcg
     8941 aaggcttgaa cttcgacgca ccaaaaacaa aagaattcaa acaagttctt gctaacttat
     9001 ctatcgactc taaagtattg gttgttctag aaaaaggtaa cgactttgct gctttatcag
     9061 cacgtaactt accaaacgtt tctgtagtaa cttctgataa tgtgagtgtg ctagacgttg
     9121 tatcaaatac aaaagtgtta gcaacacaaa ctgctcttac tcaaattgag gaggtgcttg
     9181 cataatgaac ttactagacg taatcaaacg cccagtgatc actgaaaaat caatgcttgc
     9241 catggatgac aagaaataca cttttgaagt ggacactcgc gcaaacaaaa ctttagtaaa
     9301 acaagcggta gaagctgctt ttgacgttaa agtcaaaaac gtgaacatcg tgaacgtacg
     9361 tgcgaaattc aaacgtatgg gcaaatatgc aggatataca aaaaaacgtc gcaaagcgat
     9421 cgttacattg actgaagatt caaaagaaat ccaattattc gaagctgctg aataagcact
     9481 aatagataac tcattaaatt aggagggaaa aacgtggcga ttaaaaagta caaacctacc
     9541 tcaaatggtc gtcgtaacat gacaggttct gattttgctg aaatcacaac atcaacacct
     9601 gaaaaatcat tgttacaacc attgaaaaac catgctggac gtaacaacaa cggccgcatc
     9661 actgtacgtc atcaaggtgg cggacacaaa cgccaatatc gcgtgatcga cttcaaacgt
     9721 aacaaagata acgttgttgc aactgttcaa acgatcgaat atgatccaaa ccgttcagct
     9781 aacatcgcgt tagtacacta tgaagatgga gtgaaagctt acatcttagc acctaaagga
     9841 ctagaagtgg gtatgaaact tgtttcaggt ccagaagcag atatcaaagt cggaaatgct
     9901 cttccattag aaaacattcc agttggtact gttatccaca acattgaaat gaaacctggt
     9961 aaaggtggac aattgatccg ttctgctggt acaagtgctc aagtacttgg taaagaaggc
    10021 aaatacgtat tgatccgctt gaactctggt gaagttcgta tgatcttagc tgcatgccgt
    10081 gctacaatcg gttctgtagg taacgaacaa cacgaattaa tcaacatcgg taaagctgga
    10141 cgctctcgtt ggatgcgtaa acgcccgact gtacgtggta gcgtaatgaa ccctaatgat
    10201 cacccacacg gtggtggtga aggtaaagct cctatcggac gtaaagcacc agtttctcct
    10261 tggggtcaac cagctcttgg ttacaaaact cgtaacaaaa aagctaaatc agacaaactt
    10321 attgttcgtc gtcgcaaaaa taaataatta ctttgaccat gtgtcgcaca ttttgagagg
    10381 aggttcacca tgggtcgtag tttaaagaaa ggaccttttg tcgatgagca tttaatgaaa
    10441 aaagttgaag ctcaagcagg ggttgaaaag aaaaaagtta tcaaaacttg gtcacgccgt
    10501 tcaacagttt tcccaagttt tgttggatat accatcgcag tttatgatgg acgtaaacat
    10561 gtaccagttt acatccaaga agacatggta ggacacaaat taggtgaatt tgcacctaca
    10621 agaacttatc gtggacatgc tgctgacgat aagaaaacga aacgctaatt tgaggggagg
    10681 ataaatccaa atggcagaac aaatcacatc agctaaagca actgcaaaaa cagttcgcac
    10741 ttcacctcga aaagcccgtt tagtaattga ccttatcaga ggtaaaagtg tcgcagatgc
    10801 aatttcaatc ttgaaattca caccaaacaa atctgctgga atcattgaaa aagtattgat
    10861 gtcagcagtt gcgaatgcag aaaataactt tgacttagat gttgaaaact tggtagtaag
    10921 cgaagctttt gttaacgaag gacctacaat gaaacgtttc cgtcctcgtg caaaaggatc
    10981 agcttctcca atcaacaaac gtacaagtca tatcacggta gtcgtatcag aaaaataagg
    11041 agggataatc agtgggtcaa aaagtacatc caattggaat gcgtgtaggc atcatccgcg
    11101 actgggatgc taaatggtat gctgaaaaag agtatgctga gttcttacac gaagatttaa
    11161 gaatccgtaa atttatttca actaaacttg ctgatgctgc tgtatcaaca gttgaaatcg
    11221 aacgcgcaac aggtcgcgta aacatttcaa ttcacacagc taaaccaggt atggttattg
    11281 gtaaaggcgg ttctgaagtc gaaagcctaa gaaaagaatt aaacaaatta actggtaaaa
    11341 gaatccatat caatatcgtg gaaatcaaaa aaccagatct agatgcaaaa ttagtaggtg
    11401 aaggaatcgc acgtcaatta gaaaaccgtg ttgctttccg tcgcgctcaa aaacaagcca
    11461 tccaacgctc aatgcgtgct ggcgctcaag ggatcaaaac tcaagtatct ggtcgtctaa
    11521 acggagcaga tatcgctcgt tcagaaggat actcagaagg aactgttcct cttcatactt
    11581 tacgtgcgga cattgattac gcatgggaag aagcagatac aacatacgga aaactaggag
    11641 ttaaagtgtg gatctatcgt ggagaaattc ttccaactaa aaaaaacact gagaaaggag
    11701 ggaaataatc atgttagtac ctaaacgtgt aaaacaccgt cgtgaattcc gcggaaaaat
    11761 gcgcggggaa gctaaaggcg gaaaagaagt agcattcggt gaatacggtt tgcaagctgt
    11821 tgattcacat tggatcacaa accgccaaat cgaagctgct cgtatcgcaa tgactcgtta
    11881 catgaaacgt ggtgggaaag tatggattaa aattttccct cacaaatctt atactgccaa
    11941 agcaattggg gtacgtatgg gttctggtaa aggggcacct gaaggatggg ttgcaccagt
    12001 aaaacgtggt aaaatcatgt ttgaaatcgc aggcgttcct gaagaagtag ctcgtgaagc
    12061 gttacgtcta gcttctcaca aattaccaat gaaaactaag atcgtaaaac gtgaggaaat
    12121 gggtggtgaa tcgaatgaag gttaaagaaa tcagagaatt aaccactgcc gaaatgcttg
    12181 atcaagaaaa acaattaaaa gaagaattgt ttaatcttag attccaatta gcaacaggtc
    12241 aattagaaaa cactgcacgt attaaagaag tacgtaaatc gattgcacgc atcaaaactg
    12301 tgttgcgtga acaagctaag taattatgga aggaggccat taagcgtatg actgaaggaa
    12361 gaaatcaacg caaagtttat caaggtcgcg tggtttcaga caaaatggat aaaacaatta
    12421 ccgttgctgt cgaaacaaag aaaaaccacc ctatctatgg caaacgcatg aactattcta
    12481 agaaatacaa agtgcatgat gaaaacaaca cagcaaaagt tggcgacatc gtgagaatta
    12541 tggaaactcg tccattatca gctacaaaac gtttccgttt gttggaagta gtcgaagaag
    12601 cagttattat ctaataacac gagattttcc tatatagatt gaatgcaaaa tctgaaagga
    12661 ggatacacaa cgtgatccaa gcagaaagtc gtttaaaagt cgctgataac tcaggcgcac
    12721 gtgaaatttt gacgatcaaa gtccttggtg gatctggtcg taaaactgct aacatcggtg
    12781 atgtgatcgt tgctacggtt aaacaagcaa cgccaggtgg ggttgtcaaa aaaggtgacg
    12841 tcgttaaagc cgttatcgtt cgaacaaaat caggagctcg tcgtacagac ggttcatata
    12901 ttaaatttga tgaaaatgct gcagtgatta ttcgtgatga taaaagccct cgtggaacac
    12961 gtatctttgg gccagttgca cgtgaactac gtgaaaacaa cttcatgaag atcgtttcac
    13021 tagcaccaga agttttataa tcttgagaca cttatcaaag gaggtgcgaa acagttatgt
    13081 ttgttaaaaa aggcgataaa gttaaagtta tcactggaaa agacaaaaac aaagaaggcg
    13141 ttgtactagc agcgttcccg aaaaaagata aagttattgt cgaaggtgta aatgtcgtta
    13201 aaaaacacca aaaaccttct caagctgctc ctcaaggtgg aattgttgag atggaagcgc
    13261 caattcatgt ttctaacgtg atggtggttg actctactgg tgtagctggc cgagttggct
    13321 acaaagaagt agacggcaaa aaagtccgtg tttctaaaaa aaccggtgaa gttttagata
    13381 aataaaagtt taaggaagga gggtcttact gaatgaaccg cctaaaagaa aaatatctta
    13441 aagaagtaac tccatcattg atggaaaaat ttaactatag ctctgttatg caaacaccta
    13501 aagttgaaaa aatcgtcatc aacatgggtg ttggtgatgc ggtatcaaac gctaaaaact
    13561 tagataaagc agttgaagaa ttaactttga tcactggtca aaaaccaatg attacaaaag
    13621 ctaaaaaatc aatcgctgga ttccgtttac gtgaaggaat gccaattggt gcaaaagtta
    13681 cattgcgcgg agaaagaatg tacgaattct tagacaaatt ggtttctgtt tcactaccac
    13741 gtgtacgtga cttccatggt gtaagtaaaa aagcctttga cggtcgtgga aactatactt
    13801 taggtattaa agaacaattg atcttcccag aagtagatta tgatttagta gataaagtac
    13861 gtggtatgga catcgttatt gtaacaacag caaactctga tgaagaatct cgtgagttgt
    13921 tgacacaatt aggcatgcca ttccaaaaat aattaaagga ggcgaactac gtggctaaaa
    13981 aatcaatgat tgctaaaaac aaacgtcctg caaaattttc aacacaagct tatactcgtt
    14041 gcgaacgttg cggacgtcca cattcagttt atcgtaagtt ccatctttgc cgtatttgct
    14101 tccgcgaact tgcctataaa ggtcaaattc ccggcgtgaa gaaagctagc tggtaaaaag
    14161 taaattcgca taagaaggag gtaaaagatc aatggtcatg acagatccaa ttgcagattt
    14221 tctaactcgt atccgtaacg caaacatggt taaacatgag tctttagaag tacctgcatc
    14281 aaaaattaaa cgtgacatcg ctgaaatctt gaaacgtgaa ggtttcgttc gtgatgttga
    14341 atacatcgaa gatgacaaac aaggcgtgat ccgtgttttc ctaaaatttg gtaaaaacgg
    14401 cgaacgtgtt atcacaaact taaaacgtat ctctaaacca ggtttgcgtg cttatgtcaa
    14461 agctgacgaa gtaccaaaag tattgaacgg tttaggtatc gcaatcattt cgacttctga
    14521 aggtgttgtc actgataaag aagctcgtga aaaaaatatc ggcggcgaag ttatcgctta
    14581 cgtatggtaa tcaagtagaa tctacaagga ggtgtcttta agtgagccgt atcggaaata
    14641 aagtcgttgt tatccctgaa ggagtaacag ttactcaaga cggaaacaac gttacagtta
    14701 aaggaccaaa aggtgaattg actcgtactt tttcagcaga tatcaaaatg aatatcgaag
    14761 gaaacgaagt aactttcact cgtccaaacg acagtaaaga aatgaaaaca attcatggaa
    14821 caactcgtgc gaacttcaac aacatggttg taggtgtcag cgaaggtttc caaaaagcgt
    14881 tagaactaat cggggttggg taccgtgcac aattacaagg atcaaaactt gtattgaacg
    14941 ttggttactc acatccagta gaaatcacac caccagctgg agtaactgtt gaagtaccag
    15001 ctaacacaca agtaattgtt aaaggtgcga acaaagaaga agttggcgaa ttagctgcaa
    15061 acattcgtgg aactcgtcct ccagaacctt ataaaggtaa aggtattcgt tatgttggcg
    15121 aatatgtacg ccgtaaagaa ggtaaaactg gtaaataatc gtttagaaaa attgcataga
    15181 agccagcagc aggaaaaagg catttggctg aagtttacct tcaaaaaaat acatttttac
    15241 ttgtgccgca gcttctattt ttcgaaacgt atttactgct tgaaatagta gtacaataaa
    15301 aataaagagg tgacaattgt gattacaaaa ccagataaga acaaaacacg tcaaaagaga
    15361 catcgtcgtg tacgtaacaa tatctctggt actgctgagc gcccacgctt gaacgttttt
    15421 cgttctaaca aaaacatcta cgctcaaatt attgatgacg tagcgggtgt aacgctagca
    15481 agtgcctcta ccttggataa agaaatttca ggtggaacaa aaacagaagc agcaaaagct
    15541 gtcggtaaac tagttgctga acgtgcagct gaaaaaggca ttaaagtagt agtctttgac
    15601 cgtggtggat acctttacca tggccgtgtg caagctttag ctgaagctgc tcgcgaaaat
    15661 ggactagaat tttagaagaa ggaggaatac cattcatggt ttatatcgat ccaaaacatt
    15721 tggaattaga agaccgcgtt gttgcaatta accgtgtaac aaaagttgtt aaaggtggac
    15781 gtcgtctacg ttttgctgct ttagttgttg tcggtgacaa aaacggacac gtaggatttg
    15841 gtacaggtaa agctcaagaa gttcctgaag caatccgtaa agcaattgaa gatgcgaaga
    15901 aaaacttagt tgaagtacct atggttggtt caacaatccc acacgaagta atcggtgctt
    15961 ttagtggtgg tagaatccta atgaaacctg ctgtagaagg ttctggggtt gctgctggtg
    16021 gaccagttcg tgccgtatta gaattagctg gggtagcaga tatcacttct aaatctttag
    16081 gctcaaatac accaatcaac gttgttcgcg caacagttga aggtttgaaa caattgaaac
    16141 gtgcagaaga agttgcagaa cttcgcggca aatcagttga agaattaatc ggataaggag
    16201 gacaacaata atggctgaat taaaagttac tttaaaacgc agtattatcg gacgtcctca
    16261 aaaccaacgc gatacagtta aagcgttagg tcttacaaag attaacagtt cagtggttaa
    16321 gccttctaat gaagcaatta aaggcatgat caatactgtt tctcacctag tggacgttga
    16381 agaaatttaa tttagaaaaa tcataactag aacattaagg aggtgccaaa cctatgaaac
    16441 ttcatgaatt aaaacctgct gaaggttcac gccaagtacg taaccgtgtt ggtcgtggta
    16501 cttcatctgg taacggaaaa actgccggac gcggacaaaa aggacaaaaa gctcgttcag
    16561 gcggcggtgt gcgtttagga tttgaagggg gacaaacacc attgttccgt cgtttaccaa
    16621 aacgaggatt cacaaacgtg aaccgtaaag actatgcagt cattaatctt gatgtattga
    16681 accgctttga agacggagct gaagtaactc ctgttgcttt agttgaagct ggaatcgtga
    16741 aaaacgaaaa agctggaatc aaagtattag ctaatgggga actaaacaag aaattaactg
    16801 tgaaagcagc aaaattctct aaagcagctc aagaagcaat tgaagcggct ggtggatcaa
    16861 tcgaggtgat ctaatgttta gactattaaa agatgctttt aaagtaaaag acattagatc
    16921 gaaaatccta tttactgtat ttgtgttgtt tgtatttcgc ctaggtgcac atattactgt
    16981 accaggcgta aatgctgctt cattgcaaaa cctttcttct ttgccatttt tcaacatgtt
    17041 gaacttggta agtggtagtg cgatgcaaaa cttttccatt ttctcaatgg gggtttcacc
    17101 atacattact gcttctatcg tcattcagct tcttcaaatg gacatcgtgc ctcgatttgt
    17161 tgaatggtcg aaacaagggg aagttggtcg taaaaaactg aatcaagcga ctcgatatct
    17221 gacattagta ttggcattta tccaatctgt cggtatcact gctggattca atcaatacgc
    17281 tcaattaggt ttcgtgaata atcctaacgt agcaacttac gtaacgatcg gtttgattct
    17341 aacagccggt acgatgtttg ttacttggct aggagaacag atcacagaaa aaggtatcgg
    17401 aaatggcgta tcaatgatca tttttgcagg tatcatttct agattgcctg aaagtatcag
    17461 agaaatttat gaagattact ttataaatat tgatccttca gacatatgga aatctgctat
    17521 ttttgtggcc attttactag tagcggtctt gttgatcgtg acattcgtaa catttttcca
    17581 acaagctgaa cgcaagatcc cgatccaata tacgaaacgc gtagctggcg cgcctacaag
    17641 cagctatctg ccgttgaaag taaatgcagc tggagttatt ccagttatct ttgctagttc
    17701 gtttattgct acgccaaatg ctgttttaca agcattgagc ggtagattcg gttctgaaaa
    17761 ctggtacaaa gtgatgatgg agatctttaa ctataataca gcacctggtg ctacgattta
    17821 tacgattctg atcgttgcct tcactttctt ctacgccttt gttcaagtaa accctgagaa
    17881 attagcggaa aacttgcaaa agcaaggaag ttatatacca agcgtacgcc ctggtaaggg
    17941 gactgaagag tatgtctcaa gattactgat gcgtttaagt acagttggtg cagtcttcct
    18001 tggtttagtg gcattgcttc caatcattgc tcaaatggtt tgggatcttc cacaatcaat
    18061 cggattaggc ggtacaagct tactgatcgt tatcggtgta gctttagaaa ctgcgaaaca
    18121 gttagaaggc ttaatgctga aacgtaaata tgttggattc attgagtaag aaagcgtgtt
    18181 gaggtttccc ctcaacacac gttctcacac atttattagg agggaaaaca atgaacctca
    18241 ttttaatggg gctgccaggc gcaggtaaag gaacacaagc tgaaaaaatc attgatgctt
    18301 atggtattcc gcacatctca acaggagata tgttccgcgc agcgatgcaa aacgaaacag
    18361 ctcttggcct ggaagcaaag tcttatatgg ataaaggtgc attagttcct gatgaagtaa
    18421 caaatggaat cgtgaaagag cgactagctg aaccagatac agagaaaggc ttcttattag
    18481 atggctttcc acgtacgcta gatcaagcaa aagctctgga cgcaatgcta aaagatttga
    18541 ataaaaaaat tgatgctgtc atcgatatcc atgttggtga agaaatcttg gtcgaacgtc
    18601 tagccggccg atttatctgc agcaattgtg gagcaactta ccataaaatt ttcaacccta
    18661 caaaagttga agacacatgt gatcgttgcg gtgggcatga attctatcaa agagaagatg
    18721 ataaacctga gacggttaaa aatcgtctgg ctatcaacat caaaaatagt gaaccaatct
    18781 tggcttatta taaagaacaa ggtttgctaa acactattga tggtgctcgt gaaatcgatg
    18841 ctgtatttgc agatgtacaa aaaatcattg aaaaatagca cgattgcccg tagccgactt
    18901 gcattatgca gttactcttg tcaagttcaa taaattatga tagaatatgt cagtccgact
    18961 tctagagaat cctctagatg atccattttg agaattttga ggtgggaact gcagtttccg
    19021 ccattttatc gtgtgaaaat gcgaaacaag tacaaggagg tactgtgcgt ggcaaaagaa
    19081 gatatgattg aagtcgaagg tacagtcgtc gaaactttgc cgaatgcaat gtttaaagtt
    19141 gaactagaaa acggacacca agttcttgct actgtttcag gtaaaattcg tatgcattac
    19201 atccgtatct tacctggtga caaagtaacg gttgaattgt ctccatacga tcttaaccgt
    19261 ggccgtatta cttatcgctt taaataattg tactccgtaa aactacagga ggtattatca
    19321 tgaaagtaag accatcagta aaaccaatgt gcgaacattg taaagtaatt cgtcgtaaag
    19381 gacgcgttat ggtgatttgc ccagcaaatc caaaacataa acaacgtcaa ggataattgg
    19441 aggtgctata aatatggctc gtattgcagg agtagatatt cctcgtgata aacgtgtagt
    19501 agtttcactt acttatatct atggtattgg taacactact gctaaaaaga tcttagctaa
    19561 tgctggtgta tctgaagatg ttcgcgttcg tgaattaacg aatgaacaaa cagatgctat
    19621 ccgtgcagaa gtagataaat taaaagttga aggggatctt cgtcgtgaag tgaacttgaa
    19681 catcaaacgt ttgatggaaa tcggttctta ccgtggaatc cgtcatcgcc gtggattgcc
    19741 tgttcgtgga caaaacacga aaaacaatgc acgtactcgt aaaggcccag ctcgcgctat
    19801 cgctggcaag aaaaaataat tcttaagtga aggaggttaa aacttcatgg cagcaaaaaa
    19861 agtgaatcgt aaacgccgtg tgaagaagaa tatagaagca ggtattgcac atatccattc
    19921 tacattcaat aacactatca tcatgatcac tgatactcac ggaaatgcat tagcgtggtc
    19981 atcagctggt tcattaggtt tcaaaggaag caaaaaatca actccttttg ccgctcaaat
    20041 ggcagcagaa actgctacaa aagcagcaat ggaacacgga ttaaagactg ttgaagtaac
    20101 agttaaagga cctggttctg gacgtgaagc agcaattcgt tcattacaag ctacaggttt
    20161 agaagtgact gcaattcgtg acgtgactcc agttcctcat aatggtgccc gccctccaaa
    20221 acgccgtcgt gtttaatgag tgccgctcat tttgagtttt atcagtaacg tcattgaaca
    20281 agacactttt cgttttgaaa ggggtaaaga taagaatgat tgagttcgaa aaaccaagaa
    20341 tcgcaaaaat tgatgaagaa aaagattatg gcaagttcat cgttgaacct cttgaaagag
    20401 ggtatgggac tactttaggg aattccctac gtcgtatttt attatcttct ttgccaggtg
    20461 ctgccatcac taatattcaa atcgatggtg tactgcatga attctctacc atcaaaggtg
    20521 ttagagaaga cgtcactcaa atcatcttga acattaaagg cttagcgctg aaaatgtatg
    20581 gccaagaaga aaaaaccctt gaaatcgata ttaccggacc tgctacagta actgctggcg
    20641 atattatcgt tgatagtgat gtagaaattc ttaacaaaga tatgtacatc tgtagtgtct
    20701 ctgaaggagc tacgttccat gctcgcttaa cagtgaaacc tggacgtggt tatgttcaag
    20761 cagatgaaaa taaaaaagaa gatatgccaa ttggcgttct tccagtcgat tcaatctaca
    20821 cacctgttcg tcgtgtgaac tatcaagttg agaacacacg tgttggacac cgtgaagatt
    20881 ttgataaatt gacgatggag atttggacag atggttcaat tgaaccgttg gaagcaatga
    20941 gtttgtctgc gaaaatcatg actgaacatt tggatatttt tgtgaactta actgatgaag
    21001 caaaaaatgc tgaaatcatg gtcgaaaaag aagaaacaca aaaagaaaaa atgttggaaa
    21061 tgacgatcga agagttagac ttgtctgttc gctcttacaa ttgtttgaaa cgtgcaggaa
    21121 tcaatactgt acaagaactt acaaataaat ctgagcctga aatgatcaaa gtccgtaact
    21181 taggacgtaa atcattagaa gaagttaaag caaaattgca cgacttaggt ttaggtttac
    21241 gtaaagaaga ttaaaacctt ttacgaagga gggaaaccaa cgtgagttat cgtaaactag
    21301 gacgcacatc tagccaacgt aaagcgatgt tgcgtgattt aacaactgat ttattaatca
    21361 acgaacgtat tgtgactact gaagctcgtg cgaaagaagt tcgctcaact gcagaaaaaa
    21421 tgatcacttt aggtaaacgc ggagatttgc atgcacgtcg tcaagctgcg gctttcgtac
    21481 gtaatgaagt tgccagcgtg cgtgaagaaa atgaggacat cgtcattgaa tcagctttac
    21541 aaaaactgtt caacgatatt gcacctcgtt acgcagaacg tcaaggcgga tacacacgca
    21601 ttttgaagac agaaccaaga cgcggtgatg gtgcaccaat ggttgtctta gaacttgtct
    21661 aaaatcacac taataacatc actttataga tgattctttt agagtgttat gatgttggaa
    21721 gatatttttc cgagtctagc tcagcgctgc tcctcaagcg aatatcgctt ggggagtaag
    21781 cactcatcat aaagtgtttt tttatacctt caattacctg tcatgacaga tatgtttgaa
    21841 ggcataaaaa aacattttaa gcgagaaata attgaaaatt ttgtcttttt ttgatagtgt
    21901 tagtaggaac tttaggatga agaaggagta atcatggagc caattattga attagaaaag
    21961 ataaattata aataccagcc agatgatctt cgccccgcat tgaaagatgt ctcctttaca
    22021 attgataaag gtgagtggat cgctattatt ggacacaatg gttcaggaaa atcgacactt
    22081 gccaaaacga tcaatggatt gcttctgcca gaatcaggga tcgtgaaagt gggaaatcaa
    22141 atattggatg aagaaaatat ttggacgatc cgacagatgg ttggaatggt ttttcaaaat
    22201 ccagacaatc agtttgttgg ttcgacagta gaagatgacg ttgcttttgg tcttgaaaat
    22261 caagggattc ccagagaaga aatgctagtg cgtgtgaaag atgcacttga gaaagttaga
    22321 atggctgagt ttgcttcacg cgagcctgct cgtttatcag gagggcaaaa acaacgtgtt
    22381 gccattgccg gcgtggttgc cttacgacct gatatcatta ttttagatga agcaaccagt
    22441 atgctagatc cagaaggtcg tgaagaagtg atctctacga tcaagaagat caaagaagaa
    22501 agtcaattga ccgttatatc tatcactcat gatattgatg aagcagctaa tgccaatcgg
    22561 atactagtga tgagacaagg cgaacttgtc cgtgaaggta caccaaaaga aattttttct
    22621 gctggtcctg aactgatcga cttaggtcta gatctaccat ttcctgaaaa actgaagagc
    22681 gcactgaaag aacgtggggt tgatgtgccc agtgaatata tgacagaaga aaggatggtg
    22741 gattggttat ggacatccgt tttgaacaag tagacttcac ttatcagccg aatacaccat
    22801 ttgaacaaag agccttattt gacatcaata tgacgatcaa agaaaacagt tatactgcat
    22861 tagtagggca taccggaagc gggaaatcaa ccttacttca gcatttgaat gcgcttgtta
    22921 agccaacaag cggtacagtc catattggag aacgagatat tcagccggat acggataata
    22981 agaatttaaa gcccatccga aaaaaagttg gcatcgtctt tcagtttcca gaagcacagc
    23041 tattcgaaga aacggtagca aaagacattg ctttcggtcc taaaaacttt ggtgtcagcg
    23101 aagaagaagc actagtccta gcaaaagaaa cattagaaca agttgggctg gatgaaagct
    23161 atttggaacg ttcgccattt gaactttcag gagggcaaat gcgtagagta gcaatcgctg
    23221 gtgtgcttgc catgaggccg gaagtacttg tattggatga accaacggca ggacttgatc
    23281 cccagggtag aaaagaaatg atggagatgt tttggcgtct tcataaggaa cagcagatca
    23341 ctattgtatt agttacgcat ttaatggatg atgtagccaa ttttgcagat tatgtctatg
    23401 tgttggaaaa gggacgaatc gttaatagcg gggagccgca agaggtcttt caaaacattg
    23461 aatggctaaa agaaaaacaa ctaggcgtac ctactgccac cgagtttgca gaagaattaa
    23521 tggctaaagg gatgaacttt gctaccttgc ctttgacagc tgaagaatta gcagacgcta
    23581 tcgtccatta tgctggagga aatgcccatg atgaataaat tgatatttgg tcgttatatg
    23641 cctggagatt ccttgatcca tcgattggat ccccgagcta aattattagc cagtttttat
    23701 tttatcggaa tcattttttt agcaaataat tgggaaacgt accttttgtt ggcatttttc
    23761 acattacttt ctgtcttctt gtcaaaagta gatttgaaat tttttatccg aggcattcgc
    23821 ccattgatct ggctaatcct cttcactgta gctttgcagg tcttgtttac aagtggcgga
    23881 gaaacttatt ggagttgggg tgttttccat atcactgaat ttggtgtgat gaacggcctc
    23941 tttattttct gtcgattcgt gttgatcatt tttatgtcga ctctactcac gcttactaca
    24001 ccgcctttgg aattatcgga tgcgattgag tacttgcttc gtccattgaa agccgtacgg
    24061 ttcccagtcc acgaaatttc attgatgctg tcgattgctc ttcgttttgt gcctacattg
    24121 atggacgaaa cagaaaagat catgaacgct cagcgcgctc gcggtgttga ttttggtgaa
    24181 ggaaacctag ttcagaaaat gaaagcagtc attcctttgc tgattccttt atttgtcagc
    24241 agcttcaacc gtgcggaaga tcttgcgact gctatggaag caagaggcta caaaggtggg
    24301 gaaggaagaa cgaaatatcg tgtattgcat tggcataata gagacacgat ggttatcatt
    24361 gcttttgtat tattaactgt gctactgatc tttttaagag gataaacgtt cagtgggccg
    24421 ctatctattc ttttcttgct gatctgtttg caagaaagga tgcgagcagt gtatgacaag
    24481 aaatattagg gtctgtggca gactaaggtc acaggcctct ttcttttatc tgaaaagaaa
    24541 gaacgaaaaa attttataaa aaaatcatta ttccagataa ccttttagga acaaaggaga
    24601 gaaaaacatg gtacgttaca aagcaatcat tgcatacgac ggaacaaatt ttaatggatt
    24661 ccaaaaacag ccaaatgggc gtactgttca agaagaagta gaaaaaacat tacaaaaaat
    24721 ggcaaatgga aaagaaatca cggtctttgg ttcgggaagg acagatgccg gagtacatgc
    24781 gattgggcaa gtaatccatt ttgattatcc agaggagcgt ccgttagaac gaatgcgttt
    24841 cgcgctagat acccagtcac cagaggatat tgctgtaaga caagtagaaa ttgtttcaga
    24901 agacttccac gcaagatatt tggtcaaaga aaagacgtat caatttcgag tggatatagg
    24961 gaaaccacgc agtccttttc gacgccatta tgcaagttat tttccttacc ctcttgattt
    25021 ggaaaaaatt cagcgggcgc ttcctgattt attagggact cacgatttca cctctttttg
    25081 tgcatcagga agctcgatag aggataaagt acgtacgatc tacgaggcta agatggaggt
    25141 aaatgaaaca aaagacgaat tgttgtttac atttagggga aatggcttct tgtataagat
    25201 gatacggatc ttagtaggaa cgcttttgaa aattggcaat ggccgattgc ctgaagatag
    25261 tatcccagat attattgcga aaaaagatcg taatgcagca ggacctacag cacatcctga
    25321 aggactttat ttgtatgaag tgaaatatga ataagaggtt gtgacagaag tggtcagctt
    25381 caagaaataa gaaggaattt acgaaaatta cttctcaaaa ttttgcaaaa tttcggctta
    25441 ttttcgaagg agctacttct ggaacaccgt gaatcagagg ttgtgaaaaa gctgccctgc
    25501 atcaagtaat aagaacaacc aaacaaaaat agctc
//
LOCUS       DACCKU010000036        25323 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.038, whole genome shotgun sequence.
ACCESSION   DACCKU010000036 DACCKU010000000
VERSION     DACCKU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 25323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.038"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(97..474)
                     /locus_tag="IVN13_001416"
     CDS             complement(97..474)
                     /locus_tag="IVN13_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011912.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            730..1458
                     /locus_tag="IVN13_001417"
     CDS             730..1458
                     /locus_tag="IVN13_001417"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP7011913.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1458..1712
                     /gene="purS"
                     /locus_tag="IVN13_001418"
     CDS             1458..1712
                     /gene="purS"
                     /locus_tag="IVN13_001418"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP7011914.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1714..2385
                     /gene="purQ"
                     /locus_tag="IVN13_001419"
     CDS             1714..2385
                     /gene="purQ"
                     /locus_tag="IVN13_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP7011915.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2386..4608
                     /gene="purL"
                     /locus_tag="IVN13_001420"
     CDS             2386..4608
                     /gene="purL"
                     /locus_tag="IVN13_001420"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP7011916.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4593..6032
                     /locus_tag="IVN13_001421"
     CDS             4593..6032
                     /locus_tag="IVN13_001421"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP7011917.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6064..7107
                     /locus_tag="IVN13_001422"
     CDS             6064..7107
                     /locus_tag="IVN13_001422"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP7011918.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7104..7682
                     /gene="purN"
                     /locus_tag="IVN13_001423"
     CDS             7104..7682
                     /gene="purN"
                     /locus_tag="IVN13_001423"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP7011919.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7679..9214
                     /gene="purH"
                     /locus_tag="IVN13_001424"
     CDS             7679..9214
                     /gene="purH"
                     /locus_tag="IVN13_001424"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP7011920.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9230..10504
                     /gene="purD"
                     /locus_tag="IVN13_001425"
     CDS             9230..10504
                     /gene="purD"
                     /locus_tag="IVN13_001425"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP7011921.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10626..11540
                     /locus_tag="IVN13_001426"
     CDS             10626..11540
                     /locus_tag="IVN13_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP7011922.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11673..13238)
                     /locus_tag="IVN13_001427"
     CDS             complement(11673..13238)
                     /locus_tag="IVN13_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7011923.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13231..14682)
                     /locus_tag="IVN13_001428"
     CDS             complement(13231..14682)
                     /locus_tag="IVN13_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP7011924.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14696..15712)
                     /locus_tag="IVN13_001429"
     CDS             complement(14696..15712)
                     /locus_tag="IVN13_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7011925.1"
                     /translation="MKRVLFFLALVVTAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15932..17020
                     /locus_tag="IVN13_001430"
     CDS             15932..17020
                     /locus_tag="IVN13_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7011926.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17074..18600
                     /locus_tag="IVN13_001431"
     CDS             17074..18600
                     /locus_tag="IVN13_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP7011927.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18602..19807
                     /locus_tag="IVN13_001432"
     CDS             18602..19807
                     /locus_tag="IVN13_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7011928.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20064..21041
                     /gene="guaC"
                     /locus_tag="IVN13_001433"
     CDS             20064..21041
                     /gene="guaC"
                     /locus_tag="IVN13_001433"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP7011929.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21305..22729
                     /locus_tag="IVN13_001434"
     CDS             21305..22729
                     /locus_tag="IVN13_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP7011930.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23190..24299
                     /gene="pdhA"
                     /locus_tag="IVN13_001435"
     CDS             23190..24299
                     /gene="pdhA"
                     /locus_tag="IVN13_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP7011931.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24302..25279
                     /locus_tag="IVN13_001436"
     CDS             24302..25279
                     /locus_tag="IVN13_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP7011932.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8189 a         4308 c         5690 g         7136 t
ORIGIN      
        1 ttttagttta ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc
       61 tttaccgtgc ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt
      121 ataactaaaa tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat
      181 ctcttgtcca ttgaataaga tctggatacg gtaatgattt ttgaaacgct tctgttcatg
      241 aatctgactg attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg
      301 gcattcagca cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc
      361 tttattgcat aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc
      421 ttcagctgtt aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa
      481 gtgcctcctc gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta
      541 taaatgtaaa cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa
      601 aagaatctaa tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa
      661 atttaaagtg aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga
      721 gagacgtaca tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt
      781 acttctgatg agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc
      841 gcgaggaaag accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt
      901 ttccgttatc tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc
      961 gaacaattag caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca
     1021 gcaggaagtt tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc
     1081 attgaattct actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc
     1141 tatttcttac aacttgcaaa gccggaagaa atcagtgagc tgaaagccca agcgttggct
     1201 attaatgaaa ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag
     1261 ctagaatttg gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact
     1321 tgccgattat gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat
     1381 ctaggtgatt tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa
     1441 gaaaaggagc gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt
     1501 tagatcctca aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg
     1561 aagatatccg aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg
     1621 atacagtgat tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc
     1681 gatatgaaat caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca
     1741 ggttcaaatt gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc
     1801 gaatttgtac ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga
     1861 ggattctcct atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg
     1921 gaagaagtga ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc
     1981 cagatattga cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat
     2041 tttatttgca aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat
     2101 gaagccgacg aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac
     2161 gaagaaacac ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac
     2221 atcaacggca gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga
     2281 atgatgcctc atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg
     2341 ttctttgctt ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa
     2401 gaaccgacag ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat
     2461 gaagaatatc gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact
     2521 ggactgtttt ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta
     2581 cgcaaatttc ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc
     2641 gtcgacatcg gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca
     2701 gccgtggagc cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc
     2761 agcatggggg ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat
     2821 ccaagaacga aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt
     2881 atcggcatac cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca
     2941 ttagtcaatg caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc
     3001 gctggcgtcg gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac
     3061 ggagcaactt ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta
     3121 caagtaggcg atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg
     3181 catcattcgg attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct
     3241 agtgcggaaa tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca
     3301 caaagagaaa ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg
     3361 ttgatttgtg ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat
     3421 ctagatgcag tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa
     3481 ggagaagaag tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat
     3541 aaagaaatga aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca
     3601 gttatcgaag aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca
     3661 aaacgttcaa tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca
     3721 ggaagcgatg ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca
     3781 gactgcaacg cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca
     3841 gaagctgcta gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg
     3901 aactatggtt ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga
     3961 attgctgctg cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat
     4021 aatgaaacgg atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt
     4081 gaggatcata aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata
     4141 ttaggtaaga cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga
     4201 atcgaaggtg tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta
     4261 ttgacagcca tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg
     4321 gcaattgcac tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa
     4381 ttgtcctctg ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg
     4441 gaaaataaga cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg
     4501 acggatgaag cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca
     4561 gtagcaagaa agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg
     4621 aagaatgcgg gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg
     4681 ggctccatag tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag
     4741 gaaagcttaa ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa
     4801 aattgcagca gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca
     4861 atggaagtgt ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct
     4921 tagcgcataa tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg
     4981 gggcgatctt ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg
     5041 aagaaacaga ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg
     5101 cctatttatt aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc
     5161 cattagcaat cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat
     5221 tagaagttat tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca
     5281 atgacgaagg aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga
     5341 tggaatatat ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg
     5401 cacggaaaaa tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg
     5461 ttggcgtacc taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc
     5521 cttatgaaat cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc
     5581 ctgagctaag agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag
     5641 gtaaaaaggt gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg
     5701 tccatctttt gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt
     5761 tgaaatatcc atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa
     5821 atcacacgat tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg
     5881 aagagggtct gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta
     5941 tggcttattt caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg
     6001 aatcactaaa agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat
     6061 tcaatggcaa atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta
     6121 gaacggatca agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga
     6181 ggattcggcg gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg
     6241 gggaccgatg gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca
     6301 atcgggatcg actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg
     6361 ttatatttct tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta
     6421 gtaaaaggtg tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca
     6481 gctgaaatgc ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg
     6541 atagctgaaa aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt
     6601 ggaatagcat ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt
     6661 gagcagcagc agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa
     6721 gaattgcttg agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag
     6781 aaaataaaag gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg
     6841 ttaccagaat ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc
     6901 ggctgtttac aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg
     6961 ggcatcggta tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt
     7021 gagcaagctg gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt
     7081 gtggtgttta taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca
     7141 actttcaagc gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt
     7201 tgttttgtga tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg
     7261 cagattgttt ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc
     7321 ataaattgaa agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg
     7381 gaccggtatt gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac
     7441 ctgccttccc tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg
     7501 gtgtcacaat tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa
     7561 aagtgagaat tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag
     7621 agcatcgaat ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat
     7681 gacaagagca ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt
     7741 gattgaacaa ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc
     7801 tgggatcaaa acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg
     7861 tgtcaaaaca cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag
     7921 ccatatggag gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt
     7981 atatcctttc aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa
     8041 cattgatatc ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac
     8101 ctctgttgtt gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca
     8161 tacttcactt tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata
     8221 tgatgcactg atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt
     8281 gacacttacg tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc
     8341 tgccttttat cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca
     8401 tgggaaagaa ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa
     8461 ggaatatgac gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac
     8521 agcatctacg attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt
     8581 tggtgggatc atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa
     8641 gctgtttcta gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca
     8701 aaaaaagaaa aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg
     8761 agaagaaacg gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga
     8821 aaatgaagaa gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc
     8881 attagcgttt gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa
     8941 cgaacgtcag acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat
     9001 tgcgattgaa caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt
     9061 tccaatggat gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc
     9121 aggaggaagc atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat
     9181 ggtctttact gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt
     9241 ggtgatcggt gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa
     9301 ggtggatacg gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg
     9361 tgtcgctatc agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat
     9421 tgattggagt atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat
     9481 ggcggaagga ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa
     9541 aagttttgct aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt
     9601 tgaatgttac gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa
     9661 agcggacggc ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct
     9721 ggatgcggca gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt
     9781 cgtagaagag tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa
     9841 ggtgtatccg atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc
     9901 aaatacaggc ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga
     9961 aaaagctgtg gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc
    10021 tttcacgggt gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga
    10081 attcaatgcg cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga
    10141 ctttgcgcaa gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca
    10201 agaaggatac aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac
    10261 aggaatggtt ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc
    10321 ctcaaataac ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg
    10381 tgagacgatc aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga
    10441 tggaacattt tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa
    10501 gtaaatcagc tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt
    10561 agtgaagcct tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag
    10621 ttcagatgaa aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag
    10681 gctgtatgcg tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa
    10741 acggcattga tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct
    10801 ttgcagatgc tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat
    10861 gtggaatcgt tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag
    10921 aaaaaagttt gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag
    10981 atacattggt agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa
    11041 aagttcgtta ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat
    11101 atgttcgtca accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga
    11161 tcgatcaagg gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg
    11221 tacttgatta ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg
    11281 gattttttga aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag
    11341 aagaactagc agctaaatat caaacgacac caactggctt ggcttctgct tggattttac
    11401 gccatccagc caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa
    11461 tcgctcaagc ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg
    11521 ctggaaacat tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa
    11581 gacagaataa cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa
    11641 tggaaagagt atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc
    11701 tgtgtccgat attcttttgg agaaatccct tgttttttct taaataaata actgaaatag
    11761 ttagggtcat tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc
    11821 aataattttt ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta
    11881 aacgattgag aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc
    11941 caagttaagg acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc
    12001 aaaattggct gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg
    12061 aacatataaa ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg
    12121 gtggatgtgc aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg
    12181 agtagcttag tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt
    12241 ttcgtcaagt gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt
    12301 gagaggtcaa agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca
    12361 tagctgataa tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca
    12421 aaagatgcag gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta
    12481 tttttttgtc ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg
    12541 atcacatttt cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca
    12601 gagaagatca cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg
    12661 taatcttcaa agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct
    12721 gctaaagagc gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct
    12781 ttgaacaaac cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg
    12841 tgcgtttctt tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat
    12901 tcgctacttt taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc
    12961 atataatcga aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc
    13021 cttgcttctt ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg
    13081 atgtcgggct gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg
    13141 acataagtga ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga
    13201 atgaggtatt cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt
    13261 gggagataga gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt
    13321 gataggtttt tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc
    13381 gtagccgata ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag
    13441 tttttctgca gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat
    13501 tttgcatgtg gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac
    13561 agcattttca atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc
    13621 tggtatatcg atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata
    13681 atcattgata tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga
    13741 cgtacgaaaa aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg
    13801 ttcgatcaaa gaaataatgg catccaaact gttataaata aaatggggag taatctgagc
    13861 ttgcaataca cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa
    13921 agactggttt agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag
    13981 tgtttccggc agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc
    14041 gatcagctga ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc
    14101 atagaaaaaa atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg
    14161 gacaagtgtc gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa
    14221 gatatctgac agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc
    14281 cataaaatct tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc
    14341 gatcgtacgt agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt
    14401 gatctctcga atatcattcc taagttcttc taggatattt tcctcattga actcatctac
    14461 ggttacttgg ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa
    14521 agcaattgca tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg
    14581 cgaatataac agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt
    14641 tgcttgggca aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat
    14701 aatttctggt tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa
    14761 tatctttagg taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg
    14821 ctacatacca gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta
    14881 gcttctcgat catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt
    14941 tgattgatgt ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt
    15001 ataatacatc gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc
    15061 ctttcatccg aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt
    15121 cgatggttcc atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg
    15181 agcgtagctt ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct
    15241 gtttctttga atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt
    15301 tgaaactggg gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt
    15361 cgacaagtat gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg
    15421 attcttgcaa aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat
    15481 cttggatttg ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt
    15541 tttcaagcgc agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg
    15601 attgcgaaaa accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca
    15661 tcatttggta agcggttaca acaagggcta aaaaaaataa aacacgtttc actttaaatc
    15721 actccatctg gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag
    15781 gaatcaatcg aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta
    15841 aaaaatttaa agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac
    15901 tcggtgtatc aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg
    15961 gactatcgct attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc
    16021 agatgatagt acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat
    16081 cgctgatggg aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca
    16141 atatggagaa gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg
    16201 ggtagataca cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa
    16261 agcaaaagaa gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa
    16321 tgtcgattat tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat
    16381 tgaagagcac ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg
    16441 ctcgcctgac gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc
    16501 atatatggac aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc
    16561 tacgcttcgc tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa
    16621 ttatactgac caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat
    16681 catttcgtca ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac
    16741 tggacaagat gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac
    16801 gatctttaaa gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag
    16861 tgatggagaa gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt
    16921 ccctacgtat ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat
    16981 cgacaccgat tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact
    17041 aaagagatct aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca
    17101 gatatcacca aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa
    17161 cgtggggaaa tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat
    17221 gtcttgagcg gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag
    17281 ctgtgtcaat ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa
    17341 gaattagcac taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa
    17401 gcagaaagag gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat
    17461 aaagtcggtt tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag
    17521 caactagttg aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag
    17581 cctaccgctg cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg
    17641 aaagagcaag gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc
    17701 gcagatcgaa tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg
    17761 atcagcgaag agcatattat tcgcggaatg gtgggcagag atttagttga tcgttatcct
    17821 gaaagaaaac cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac
    17881 cctcttgatc acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag
    17941 attgtaggaa tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt
    18001 ggacattctt atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca
    18061 gttaagaccg ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa
    18121 tctttaggac tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat
    18181 aaaataagtc aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc
    18241 cggaaaaaga tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt
    18301 gggaatcaac aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt
    18361 ttggatgagc caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc
    18421 gaagaaatgg cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata
    18481 ttagggatgt gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg
    18541 cgagaagatg cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa
    18601 gttggaacag gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag
    18661 agcagctgat ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc
    18721 gtttcaattg atgacggatg gtattttctg gcgtccattg aatataacta accttgttct
    18781 gcaaaatagc catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca
    18841 tgtggatttg tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat
    18901 ggtcaatcgc caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct
    18961 gatcggcgca tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac
    19021 tttagcagga ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct
    19081 agcacctttt ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga
    19141 aggaagcagc cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt
    19201 tgttggtcaa tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc
    19261 aacagctttc ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat
    19321 ctttgcctct tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc
    19381 atatgggttt ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa
    19441 tcgaaaagcg gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt
    19501 caacatggga gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc
    19561 aacacctcaa gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg
    19621 tgcttcgact tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat
    19681 gggtgtattg aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat
    19741 caaaggattg atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac
    19801 aacgtaggag aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct
    19861 ttttttcgct aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt
    19921 ttttttacca ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa
    19981 attacatagg gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat
    20041 gtgaacatca ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc
    20101 ctaataaatg tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac
    20161 atacattcaa aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg
    20221 cagaattctt agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc
    20281 gaattccgtt tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg
    20341 tcaagaaaga ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt
    20401 atatcactat cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata
    20461 ttaaaaaata tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg
    20521 tccgtgaatt ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag
    20581 tatgtattac aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta
    20641 gatggtgtgc gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg
    20701 gcgatattgc aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg
    20761 ccggtcacga ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat
    20821 actttggttc tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa
    20881 tctggatcca acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc
    20941 aatcatctat ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg
    21001 tcatcgtgaa aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag
    21061 gatgttgtga aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct
    21121 ttatatgttt tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt
    21181 cagaataagg gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt
    21241 tttctttttt ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa
    21301 gcttatgtgg gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca
    21361 gattatgaga gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg
    21421 aaaactaggg caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct
    21481 tagggagctt ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga
    21541 atgggggaaa ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga
    21601 gcagacggac tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt
    21661 tcttgattta tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc
    21721 ttattctctt gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc
    21781 gttacgtaac aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa
    21841 ccagcttctt gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct
    21901 ttcagctgga gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt
    21961 caaagcttca ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga
    22021 aaaactgaaa gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt
    22081 tcaaaaccct acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg
    22141 tcgacagtat cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa
    22201 ccaagaagaa gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc
    22261 gttatctagg gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt
    22321 tatctcgcaa ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca
    22381 aatcacagtg gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa
    22441 aaagttgaaa gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa
    22501 gcttcattca attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga
    22561 ccccgaacta gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt
    22621 tgggcaagat ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc
    22681 gttgttttat tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt
    22741 aaatagatta aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg
    22801 aaaaagaata cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat
    22861 gtgtaaataa aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa
    22921 cagatggttg taagtttgat tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt
    22981 ccttattgtg attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca
    23041 tcgtgaaaat ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga
    23101 agacggttaa atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt
    23161 cttatagatg agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc
    23221 aagcactgct agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg
    23281 gaaacgtagt taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa
    23341 tgactcatat ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag
    23401 gacgcctagg cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt
    23461 acgcatttga taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa
    23521 aacatggttt acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt
    23581 cttatccgga agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac
    23641 aagcagccgg tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt
    23701 atactggtga tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg
    23761 cttaccatgc aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc
    23821 gtgaaaaaca aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg
    23881 gtattcaagt cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg
    23941 aatgggcgat cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac
    24001 cacatacact atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt
    24061 ggcagaaaaa agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt
    24121 ctgaagaaaa agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg
    24181 ctgaagccga caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag
    24241 tatctccaca atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat
    24301 catggcacaa aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa
    24361 tgacgagaat gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc
    24421 aactgaagga ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga
    24481 atctggaatc gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga
    24541 aatccaattc tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg
    24601 tacaagatat cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg
    24661 tggcggtgtt catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc
    24721 tcctggtatt cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc
    24781 ttctattaga agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt
    24841 ccgagaagaa gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg
    24901 tgagggaaca gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc
    24961 agcagacaat cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc
    25021 gccattagat gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt
    25081 ccaagaagca caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg
    25141 cgctatcctt tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc
    25201 atttggacaa gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa
    25261 agaaatcgcg gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa
    25321 att
//
LOCUS       DACCKU010000037        24935 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.039, whole genome shotgun sequence.
ACCESSION   DACCKU010000037 DACCKU010000000
VERSION     DACCKU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24935
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.039"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(12..2156)
                     /locus_tag="IVN13_001437"
     CDS             complement(12..2156)
                     /locus_tag="IVN13_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7011933.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2513..3886)
                     /locus_tag="IVN13_001438"
     CDS             complement(2513..3886)
                     /locus_tag="IVN13_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7011934.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3886..4281)
                     /gene="rbsD"
                     /locus_tag="IVN13_001439"
     CDS             complement(3886..4281)
                     /gene="rbsD"
                     /locus_tag="IVN13_001439"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP7011935.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4309..5226)
                     /gene="rbsK"
                     /locus_tag="IVN13_001440"
     CDS             complement(4309..5226)
                     /gene="rbsK"
                     /locus_tag="IVN13_001440"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP7011936.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5334..6347)
                     /locus_tag="IVN13_001441"
     CDS             complement(5334..6347)
                     /locus_tag="IVN13_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7011937.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6428..7027)
                     /locus_tag="IVN13_001442"
     CDS             complement(6428..7027)
                     /locus_tag="IVN13_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7011938.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7213..7914
                     /locus_tag="IVN13_001443"
     CDS             7213..7914
                     /locus_tag="IVN13_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7011939.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7924..10635
                     /locus_tag="IVN13_001444"
     CDS             7924..10635
                     /locus_tag="IVN13_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7011940.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11086..12714
                     /gene="treC"
                     /locus_tag="IVN13_001445"
     CDS             11086..12714
                     /gene="treC"
                     /locus_tag="IVN13_001445"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP7011941.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12774..13955)
                     /locus_tag="IVN13_001446"
     CDS             complement(12774..13955)
                     /locus_tag="IVN13_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7011942.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14207..15730
                     /locus_tag="IVN13_001447"
     CDS             14207..15730
                     /locus_tag="IVN13_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP7011943.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(15799..16527)
                     /locus_tag="IVN13_001448"
     CDS             complement(15799..16527)
                     /locus_tag="IVN13_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7011944.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16540..17820)
                     /locus_tag="IVN13_001449"
     CDS             complement(16540..17820)
                     /locus_tag="IVN13_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7011945.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17961..18563)
                     /locus_tag="IVN13_001450"
     CDS             complement(17961..18563)
                     /locus_tag="IVN13_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP7011946.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18808..19014
                     /locus_tag="IVN13_001451"
     CDS             18808..19014
                     /locus_tag="IVN13_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011947.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19326..20045
                     /locus_tag="IVN13_001452"
     CDS             19326..20045
                     /locus_tag="IVN13_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7011948.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20126..20809)
                     /locus_tag="IVN13_001453"
     CDS             complement(20126..20809)
                     /locus_tag="IVN13_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP7011949.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            21027..23213
                     /locus_tag="IVN13_001454"
     CDS             21027..23213
                     /locus_tag="IVN13_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7011950.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            23559..23969
                     /locus_tag="IVN13_001455"
     CDS             23559..23969
                     /locus_tag="IVN13_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7011951.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            23983..24339
                     /locus_tag="IVN13_001456"
     CDS             23983..24339
                     /locus_tag="IVN13_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7011952.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT         8352 a         4427 c         4864 g         7292 t
ORIGIN      
        1 tttaatttat tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac
       61 aaggttagta caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt
      121 cttgccgtaa aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg
      181 ttgttgacgc gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc
      241 catttgattg gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca
      301 gttgttgctg cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc
      361 gcgttcaatt tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt
      421 gcttctttgt agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca
      481 gtcagtccat ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat
      541 ttacggaatt gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg
      601 aaggtcacgc tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg
      661 cttgataata agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat
      721 gcgtagattc cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaat
      781 tgatcagttg tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat
      841 gagccgcctg tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca
      901 aaatcaggcg tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca
      961 tggataaatt gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt
     1021 ccccagaaga cgaggtcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat
     1081 tgctgttcgc ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa
     1141 gtcaaaccag aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca
     1201 agatgcttga atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat
     1261 acagcatcaa ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata
     1321 ccgctttgat ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa
     1381 ccattgtttg gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg
     1441 tttgtttgat tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt
     1501 gaaccattat tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt
     1561 gtactaggga cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct
     1621 ttaaattctg cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa
     1681 tttacttctt tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta
     1741 atagtgtaag ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata
     1801 tccaaaggtt gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc
     1861 aaagaaggag caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca
     1921 gccggatcaa tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg
     1981 tcttctgtaa aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt
     2041 gtgactgttt gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca
     2101 gacggagcaa tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata
     2161 aactagtcct tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt
     2221 ggaacagata atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa
     2281 taaaacaatt tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat
     2341 tgcatagacc acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt
     2401 aaattatacc aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag
     2461 aaaaagactg aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat
     2521 aagacattag gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac
     2581 taacagccag tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg
     2641 tcagactacc aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac
     2701 cagcagctgc accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt
     2761 aaacagttgg ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg
     2821 aacgggtttt tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa
     2881 aattggctgc tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga
     2941 tcatgacagg gtttacagcc cattggatac caaatattac caaaatattc caaccaccag
     3001 cgatgattgc gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta
     3061 aaccttcgcc aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga
     3121 taactaatga aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga
     3181 ggaatttttc aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg
     3241 tatactgcat cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg
     3301 aaagtaaaga ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc
     3361 cattcacttt aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga
     3421 cggcattcga cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa
     3481 tcgtagtcgc taaaacaacc agacctttta atataccaga accggcaaac agcggaatga
     3541 tcggcgtaaa gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt
     3601 tcggagctga atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat
     3661 ccatgattat ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct
     3721 gttttaattt ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg
     3781 cacatggaag gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa
     3841 tctgctcgga cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc
     3901 actcactaat ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca
     3961 gttattcaac tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa
     4021 caataatttg attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc
     4081 aatcgattgt acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag
     4141 accgtttgag acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc
     4201 cccgatactt aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt
     4261 gattactttt ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga
     4321 ttgttctaaa gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac
     4381 agaagaggct agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag
     4441 tgtgctgctt aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac
     4501 tttaaaagca gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc
     4561 tagtgtaatg attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa
     4621 actgttatga tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat
     4681 aatgatatct gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt
     4741 caggatcgtt gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa
     4801 gctactttca aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag
     4861 gacttcttgt tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc
     4921 tgctgcgtct accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc
     4981 agatgtatcg atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc
     5041 cccaacgcct ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt
     5101 ggcaccttta cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt
     5161 gggcatgttg gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt
     5221 gttcatttta caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa
     5281 cgttttagta aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc
     5341 aagggtgcag ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct
     5401 ttttctggga attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag
     5461 attggttgtg cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca
     5521 tagccaataa tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat
     5581 aatccaaaag ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt
     5641 tctgctgtga tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg
     5701 tcgtgaggga tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca
     5761 ttttccggtg gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag
     5821 tgcatgcccg ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct
     5881 tcagattttt tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg
     5941 attgcatctg tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg
     6001 agctcggcta gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg
     6061 acgaaattct gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga
     6121 ttcgtgatat caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta
     6181 gcaaaatagt caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt
     6241 ttcggactga atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg
     6301 ccggaaagtt tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta
     6361 tctaaatcct gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg
     6421 ccagtactta tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg
     6481 cagttttcgg aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc
     6541 aagcatcttc agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat
     6601 atacaccgtt tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa
     6661 gttgaagatc aagagaagta ccaaaaaaag cacttggatt gtattttgtg atagtcaaca
     6721 tattatctgt atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag
     6781 aagcagtcat tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta
     6841 cgttcactaa aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt
     6901 ttgtcagaat agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt
     6961 gcatgtcgat cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat
     7021 cattcatgat gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg
     7081 tttatttgtc aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa
     7141 ataaataagt gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat
     7201 ggaggaatca gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga
     7261 gaaacgacca ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg
     7321 attatacttg gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg
     7381 gatacacctg atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat
     7441 aaacagctta ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc
     7501 gttcctaatc taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg
     7561 cttgatcctg tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg
     7621 tcccaattat caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat
     7681 cccaaacttc ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa
     7741 gtattgaaat tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca
     7801 cataattctg ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta
     7861 aggtctgttg aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat
     7921 tttatgaaaa caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga
     7981 cgattcattg caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa
     8041 actgctggac ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct
     8101 gatttgcaaa tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag
     8161 atcccagatg caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac
     8221 gaagtgattc aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact
     8281 gatggaaaac tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa
     8341 gggtataaaa tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat
     8401 accatcacgg gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg
     8461 aacgtcggaa atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca
     8521 gatatttatt ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc
     8581 aaagcttaca aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat
     8641 cgacccgaag aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca
     8701 cagcaaaaaa tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat
     8761 gctaaaaaac agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga
     8821 acaatcgctg ttcaaacatt atctcaacaa gaagctcaac ttgctgaaca agaaaggcaa
     8881 ttagttgaga aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa
     8941 aaaattgaaa atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt
     9001 tttcaagaat ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt
     9061 ttctttttct ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa
     9121 aaccgcaggg aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa
     9181 aaatatgcaa tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta
     9241 ggaacgaatc ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg
     9301 agtgcgctta ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt
     9361 gccgcatttg gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca
     9421 ttgcttcaac cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg
     9481 ctgtggtcac gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag
     9541 tcaagaatgc ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc
     9601 gtgggattga aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac
     9661 tatcaagcaa tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat
     9721 atgctgcaga agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa
     9781 ttgagaaaaa aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct
     9841 gatttggatc gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa
     9901 acaggaatcg ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat
     9961 atttcgcttt atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa
    10021 aactatctag gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa
    10081 tcgatgatgg ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa
    10141 atgctttccg agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa
    10201 caaatcgaaa cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc
    10261 gtattatcag gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc
    10321 gagcgtgtac gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg
    10381 atgtacattg ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt
    10441 gtaggatata tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt
    10501 ccattagtga tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg
    10561 gttattgtga tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg
    10621 aaatcgaatg aataaaaaca gaggttgtga aatcagcggt ttgacctgag tattagagaa
    10681 acaaacggca aaatagtttt tcatattttg ccgttttttg agctaatacc gaaggacagc
    10741 tgattgaaca ccatgaatca gaggttgtaa aaaagccgtc ctgcatcgag caaaaagaat
    10801 actcaaataa aattagctcc tcatgtttta catttgagaa gactgattgc cgtagataaa
    10861 agcttttgaa caccatttat agaagaaaca agaatatttt gctattttaa acaagaggat
    10921 agacaaatac atgtctgatc tcttgttttt tattgggcaa cagacctctt agaatgtata
    10981 aagataaatt ttacaaacgc tttcggttca gttataataa taaatgataa cttgtatata
    11041 taagttgtgg taggtaataa atcagttgaa tggaggtaaa tatccatgac atttaaagac
    11101 aaagtcattt accaaatcta tcccaaatct tacaaagaca gtaatggtga tggaattgga
    11161 gatttaaaag gaatcaaaga aaaactcccg tatctgaatg atctaggcat cgacatgatt
    11221 tggctaaacc ctatttatcc aagccctcaa aaagacaacg gttatgatat ctctgattac
    11281 acagccatcg atcctatctt tggtacgatg gaggaattcg aacagctaat agccgaagca
    11341 aagaaatacc ggatagaaat catgctcgat atggtgctga atcacgtttc gacagaacat
    11401 gaatggttca aaaaagcact agcaggggac cagcattatc aggacttctt cattttaaga
    11461 gaagagccaa cggattgggt atcaaaattt ggtggaagtg cttggtcgcc atttggagat
    11521 acggggaaat attatttgca cttatatgat cggacacaag ctgacttgaa ctggcggaac
    11581 caagcagtaa gagaggaact attcaaagtt gttcgttttt ggatggaaaa aggggtaaaa
    11641 ggattccgtt tcgatgttat caatgttatc ggtaaagacg aacaattgaa aaataataaa
    11701 gaaaatgatg gcaaacctga atatacggac aaaccaatca cccatacgta tctaaaagaa
    11761 ttgaaccaag caacgtttgg gacggattcc actatcatca cagtcggtga aatgagctct
    11821 acgacgatcg aaaattgtat cttgtataca gaacctagta gagaagaatt atcaatggtc
    11881 tttaatttcc atcatttgaa agtcgattat gaaaatggga ataaatggtc tactcctcca
    11941 tttgattttg cagaattaaa gcgactattt catacatggg gagaaaagat gagtgagggt
    12001 aacggctgga atgctctatt ttttaataat catgatcagc cacgtgcgct caatcgattt
    12061 gtagctatcg atcagtatcg aaaacaagga gcagagatgc tagcaaccat gatccatttg
    12121 aaccgtggga cgccctatat ttatatggga gaagaaatcg gcatgatcga cccagactat
    12181 tcatcgattt ccgactatat tgacgtagaa agtttgaatg cttataagtt actccttgaa
    12241 caaggattca cagaaaaaga agcgtttcgt cggatcaaag caaaatctcg cgataactct
    12301 cgaacaccca tgcaatggtc agacacagaa caagcaggat ttactacagg gaaaccttgg
    12361 ttaaaagttg gaggcaagct agaagagatt aatgtagaaa aagaactatc ttctgaagat
    12421 tctattttgt cctattacaa gaaattgatt cggctgagaa aaacatatcc aatcgttgca
    12481 caaggagatt atcatgctta tgaagcaaat catccggaag tttatggctt tcttcgccaa
    12541 tttgaaggac agcagctgct tgtattaaca aatttctatg ataaagatac aaggatcgcc
    12601 atcccagaag gattcacaga agccaatatt cttatcgata attattccga tactgttctt
    12661 catcaaagtc tggagctaaa accataccag gcaattgctt tgctgaaaaa ttaaaacaaa
    12721 aacacaaaac gtcgttccta cgagctattc tcgtaagatc gacgttttgt gttttattta
    12781 aaataatctt cttcagaaaa tgaagagttt tcatactctc ctttatcgcc ataaaattta
    12841 ttgtagcgtt gtttgaattt acggaaaata aaagtaacga gtaatttgac taacgtatag
    12901 ataggaatac caaaaataac gcccacaatt cccattagat cacccatgac aagtaaaacg
    12961 attaagatcg taatcggatg gatctgtaaa cgatcaccca ttactcgagg aatcaccaag
    13021 tcaccgtgga gaagttgtac ggcaaaccag acaatgacaa atttgacagc cataaaagta
    13081 gagtcttgga atgcaatgat caaacctgga aagaaggcga taaacggccc aatatatgga
    13141 atgatacata acaacccagc agaaatagct aaaacactag caaattctaa tccgatcagt
    13201 aagaaacaaa cccagtagac tgctccaagg ataaatgaag caatgatttg cccctttaga
    13261 aatgaaccca attgttgatt agcaatcttt gtcaaatttt taaaatctgt acgaaatgct
    13321 ggtgggacaa tcgctaaaac tgactgataa aatttttgag gatctctcaa aaggaaaaaa
    13381 gcgacaattg gaccagtaaa taaagtaata aaaatagctg taacagttga aaaaatgttg
    13441 ctgatccgtt gtgcgccact ttcccaataa tttccaataa atccagctat atcagacgtc
    13501 acgtcgtcaa tagaagcgaa aaattgatta aatgaatcgg caaaaggtgt tttctccaaa
    13561 aattgtcgga gactctcttg aaaatcttga aacaaatctg gcaaactact gatcaatgtc
    13621 tttgcctgtt cttcaagaaa aggaataagc catatgcttc caagccccaa taaaaataaa
    13681 ataatcacat aaacgataat gactgacaag ttcctcgaaa ggtgcttgct caaacgtttg
    13741 atgactggtt ccaaaagata atacaagatc agtgcaaaca ataatggggg caagatagta
    13801 gcgataatga ttccgacagg cttgaatata aacgagatct gatggaaaaa ataaatgatc
    13861 agcgcaagta agataactat tcctaaaaca tagtaagagg tttttcctcc taaaaagcgg
    13921 atcaaacgat tcgttgtatc tgtttctttt ttcactcttt ttcctccttt atctataact
    13981 tcattataga caaaagcggg tataaaagga aaatgataga ggttgattgc aaaaatatcg
    14041 tcaatcattc atataaactt tctcatcaat taaaataaga aaacgcttga cagttctttt
    14101 aaccatatga taaattaaat cctgttgcta aaacgtaact attacggttt gcgaaaagcg
    14161 aatcggaaca ttcaaatgaa agaagatgag aagaggagtg tcataaatga aacgattgat
    14221 aggtatattg gccatgctgg ttttagcagg tatgttaact gcttgcgggg caagtgggaa
    14281 agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccca tgtatgattt
    14341 tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag cagggactga
    14401 ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata cagatatctt
    14461 tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa cattacaaga
    14521 aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg gaagtgaaga
    14581 aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc tagacccaca
    14641 tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg atcagctgat
    14701 tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct ataccgagaa
    14761 acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac aaagatcttt
    14821 tgtcacgcaa catgctgcat tcaactatct agcgttagaa tatggattga atcaagtatc
    14881 gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg aactgaaaca
    14941 ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca aagatagtat
    15001 tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc ctttggaagg
    15061 attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg aagcaaacct
    15121 cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt tgacccccaa
    15181 aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag atcgctctct
    15241 ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg ggacttttga
    15301 tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag aatataaaga
    15361 atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg agaaagagat
    15421 ggcatttacg tttaagaatg gaacaacgaa acgttcaaat tatcgttatg tagggaaaga
    15481 aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag cagaagataa
    15541 ggaaagtggc gcttttagat atattcaatt cagtgatcac acgattgcac cggctgactc
    15601 cgaacatttt catttatatg caggaaatga aagtcaagaa gtattgctga aagaaatgga
    15661 gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac aggaaatgct
    15721 tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctggtcat ttagtcggtc
    15781 caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag aggctgggat
    15841 ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc gcacattttg
    15901 tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt tctcgatttc
    15961 tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca gtttatgtgg
    16021 cgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt tgatctcatc
    16081 attaaactct attttgaaga tatctttttg gatttgggac atttgaaaat cttcgaaaat
    16141 tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt tgacgatata
    16201 atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat gggaagtcac
    16261 atagattatt tttcctaaag gatctaattc acggatcaat ttaccgatcg ctacaccatc
    16321 catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag aagttctgat
    16381 cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat aactgtctaa
    16441 gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt gttttggatc
    16501 gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt gtatataaat
    16561 cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag gattggtctt
    16621 tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg tttttgttga
    16681 gaaccctttt tgtttcaact ggtttaacgg taatgtattt gttgttgtat tgatcacacg
    16741 aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct tctttgcagc
    16801 actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa ggtttaccgt
    16861 atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgatca accctttttc
    16921 ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt tcatattatc
    16981 caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta atatcgtttg
    17041 atcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt gaagagaaaa
    17101 aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt tttctagatc
    17161 tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag ctaatttctt
    17221 attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat atagaatgaa
    17281 aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg tataaaaaat
    17341 aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac ggcggtccac
    17401 agtgtgaaac caagtctcta acgatttttt taaacaaaaa gctaataaag cagataaaaa
    17461 agcggataag acaatgtgaa aatactgcaa tgaatctcct ggttcaataa ccgaagattt
    17521 caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg tcaataaccc
    17581 aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa aagtaagcat
    17641 cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga cccatccaat
    17701 caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga tgtgatggtc
    17761 aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc ataaaaacat
    17821 aaagtacagc tcccattgat ttttaccaaa ttatagcaca attggcgtat tattttttta
    17881 atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat acaaaaaagt
    17941 cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc tccaaaattc
    18001 atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc aagtagcaaa
    18061 taggtagaaa agtaagaagc tgagaaaaat aacgtaatga gagaaaaaat tagaaacatg
    18121 ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt gattgctgta
    18181 cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa gaccatcatt
    18241 gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca taaataaagt
    18301 agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc atatctgcgg
    18361 atgcagatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt caccagagaa
    18421 aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa gacaaatttt
    18481 acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc ttctaataat
    18541 ttgttcacaa ctttatcttt catctcatac gtacctccac cacaaatttt atgctaaaaa
    18601 aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc gtcatgatct
    18661 cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt ttgatacgaa
    18721 aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa aattactgac
    18781 cttataaaag gtgatggagg gattcttatg gaaaatgaac aattgtttga agtcggcgaa
    18841 acatatgcat ttaaatctga tttatttgaa tcaccaataa aaggaaaaat agaaaaaatc
    18901 tatgaaaata gtgtgatgat acgtgtcatg aaatgcgcag tagaagacaa tgataccgct
    18961 cataatctgc actggcagtt agttgtacgc aaatcaagta caatagaaga ataaaaacag
    19021 ataaaatgtt gagagcataa agaggttgtg agtctcccgt ttagctctga gttacaagga
    19081 ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccttgtgacc gaggtgttgg
    19141 caggagaata ctgtttattt gaaaaaaggg ctgcgacaag acttttgtca cagcctttat
    19201 tttcatgaat aggagttact tttggctact tttagagaag tagcttttaa ggagcagata
    19261 aagtcaaggc ttttatttct ataaatgaag tagtaaaatg aagaacagat tttattacag
    19321 gaaggatgcc gataattgtg atcattgttg caggaacgat cggtgcaggg aaaagcacac
    19381 taactgaaat gcttgcccaa gatttagaaa caaaaccttt ttatgagaac gtggaagata
    19441 atgaagtatt gccgctattt tattctaatc cagaaaagta tactttttta ttacagattt
    19501 tctttttgaa caaacgcttt ttggcaatca aagatgcttt cagccatgat gacaacgtac
    19561 tagaccgctc catctacgaa gatagtatgc tgtttcattt gaatgcagat ttagggcgtg
    19621 tttctgaagt agaagtaaag cagtatgaag gtcttttaga aacgatgctg aaggagttag
    19681 aagaaatttc gccacaaaag aaaccagatc tactggttta tattcgtgta tcctttgaaa
    19741 ctatgttagc gagaatcaaa aaaagaggcc gggaatatga acagctagaa caagatcctg
    19801 aactctattc ctactataaa gaactcaatc gacgatacga agagtggtat gaacagtttg
    19861 atatatgtcc caaaatagtg atcgatgggg acaaatatga tttcgtagcc gatccggcat
    19921 gtggagaaca aatcgtacaa gaaatcaaga tgcgggcaaa gaaaatgaca gaagaggcag
    19981 atgcccagtc gatccgctac actacaaata acccgaaagc aaagggaatc tttacaacta
    20041 aataatataa aaactaagca aattgctcac tcgcagatat ttctctgtga gcagcatctt
    20101 gcttagtttt tttgattcat tgagatcaaa tagtgaaccg attcgtatga taaaattgcc
    20161 ataaatgaaa tgtctgagtg agggccatga tcaaaaataa aatggaaacg atcaaaatcg
    20221 aatgggcgcc aaacagatga tgacagaaaa caaggacaat agctcctgcg ataaaggcaa
    20281 gaacgacact tagatagtgg aaaaatcgat caaaatctgc tttttcttta gaaatacaat
    20341 aatcatataa gttcgttgct acccctttta aatttccagt tgtaaataaa ttagtgtagg
    20401 cacgcccgtt gattttatca aaagctaccc actggatcga tgcagtaaaa gcaatcaaaa
    20461 tcgttactaa tgcgggatgc tcatggacgg gtgtcaaact aacaaagaaa aaaatcactg
    20521 cttcataata tagaacaaac aagcgccaga aatgtttttc tttttttcta aaataatcaa
    20581 ttagatactt tgttagaaga atccctaaga aaaagaaaag ggtagacaat acttttttac
    20641 caacacttag ccattctccg tcaaatgctt ggatgatcga tagaatgata ttgccagttt
    20701 gagcagaggc aaaagcacca tattggatat aagtataact gtccatggct cctccgataa
    20761 atgtcagcaa aagtcctatt gtacgcccct cgtgaaagga agtctgcatt tttttcatca
    20821 ccttttatga atatttttgt cttccttatc ttagcataaa cgattgaaaa aaaggaaaaa
    20881 ttttcagaaa actaaaatca tatcgcttcc cattttcatt tttagaaagg tctgttagta
    20941 taaagaaaaa atagacaacg aaactttaaa taaccaactt gaaataaaca aattcaaaga
    21001 agaaagatgg gaaggaaatc aatcagttga atgaaaaaga aattctccga ttacaaaaag
    21061 aattgaaacg ctgggagaac aagtataaaa agcttcaaca agtctttcgg ctacaagaag
    21121 aaatcattgc tacctatgaa gcggcttatg gcgaagcacc cggacttatt gaggaagaag
    21181 tacaagtaaa agaaagtgaa ggaaacagct ctttctttaa aatgatggag aaacgttcct
    21241 ataatcgctt caatcaagaa caaaaagcag ctatcacata tgatatgaag aaacatcttc
    21301 gaatcatagc aggagctggg agcggcaaaa cgcaaacaat ctgtgcaaaa gctgtttatc
    21361 tgatgacgca aaaacaagta gacgaagaac gcatactaat gatcactttt acaaggaacg
    21421 ctgcaaatga actaaaaaaa cgagtagata atttcagcca gagaaaaacc gctgtccata
    21481 tcggaacatt ccatagtatc ttttttagga tatacaatga aatttgtcga aaatttcctg
    21541 aagtagcaat gcaaggaata caaggagact tttcaaagga tactgcacaa aaagtgaatg
    21601 ctgttttgca acagctgata cgtaaatata atctttatat atttgatcaa tatggagaaa
    21661 aaacgattgc ttctcggtta gactattggc agaatatgaa cctttcagta gaagaaatgg
    21721 ttcagttagt taaagaaaaa tatgattcca ttgataagaa tgcacgacag ccgatatcag
    21781 aacgactgta tggattgctc acggaactcc aagaacaaaa aagaagccag caattgctag
    21841 agttcaatga tgtactccaa aatctgaaaa gcgctttgga gaatgaggag atccagcatt
    21901 atatcggcca aaaatatgat tatctattca ttgatgaatt tcaagatact aatccacttc
    21961 aatgggaaat cgtgaaacgg atgacaaagg aaaacaggat caagttgatt gttgttggag
    22021 atgatgatca gagtatctat ggattcagag gatcagagcc agcatacatc aagaactttg
    22081 aaaaagaata tcctaccaaa actcttttct tattaactaa ttatcgttct cgggcagcta
    22141 ttgtccaagg agcaaataga ttgatcagct ataataaaaa tgatcggata ccaaaggcga
    22201 tgattccagc acaaaaagaa gcaggtgtta tggaagccta tttgttttct gatactaaag
    22261 cagaaagtca gtggctaatc aaacaaattc agacatttat tcaaaaaaat gggaaatata
    22321 aggaatcaat catattatac cgatcagctt cacaaacgca gcagcttgtc caatctctgc
    22381 tgaaaacgaa tattcctttt gtactggaag cagatagtcc atatgaaggt atctttggta
    22441 tcaagtcttt ccaacatttc tatcaaaaaa ttgttcattg gcaaacagca ctgaatctgc
    22501 aaaagaaaaa tcaggcctac cagcaattgc tccggcagct gatggcagac tgctacctga
    22561 aaaaaagtga atgcgatcaa tacttttctc ctaaatatca aaatatagcc gttgcagatt
    22621 atattttgtc gataagcccc aatttaaaat cgaaatctgc agaaatccga gagtttgagg
    22681 aagcgttgcg taaaacagac cagcatcaag aaatctattc gcttgttcaa agctatcttc
    22741 atctgccacg tatagctaag gaaatcgacc agagtgatca agaatggata cgagaagaag
    22801 taaagcagca tgctactttc agaagactcc aagccttatc gcaagaaacc caagcgacag
    22861 ctaaagaatt aaaaacccgc ttgattgctt acagacaagg aaaacttgat gcactatgta
    22921 tccagagcat tcacaaaagt aaaggtctta gttaccgaaa tgtctttttg attggttgta
    22981 acgaaggaag tttgccttat aacggagcga cagaaaagaa agtaatcgac tttaatgaaa
    23041 taaaagcaga accagtaacg acgatagaag aagaacgaag actattttat gttgcgatga
    23101 ctagagcaag aaatcgtctg tatctatgtg tacctcaaat gaaaaattca agaaaattga
    23161 aagtatcgag atttatcaaa gaaaccggga gtcgtgtgaa aaaaggaaag tgatagaaga
    23221 gagggtgtga cagaagtggt cagtttcaag aaagaagaag gaatgtacga aaattgcttc
    23281 tcaaattttt gtgaaatttc agcttatttt cgaagaagct atttctggaa cacagtttat
    23341 tcggaaaagg gaggtgtgac aagaattttg tcacacctcc cttatttatt ttgaaaatga
    23401 gaatactatt atcaaaatag agaatatata gaaaaattct cattattcaa acgtaaataa
    23461 tcagttattt tgattttttt tatattttat tagctaataa ttggtatttt aaaagaaatt
    23521 actgtagaat tggattataa ttaaaaagga gtgagaaaat gataaaagta tatacttcgc
    23581 ctagctgtac ttcctgtaga aaagcaagag catggttgca agcgaatcaa cttgaatttg
    23641 aagagaagaa tatttttgcc gaaccgttaa cagaaagtga gatcaaaaag atcttggcag
    23701 cgactgaagg aggagttcaa gatattattt ccactcggtc gaaaatttac gaaaagctgg
    23761 gcatagattt taacgaatta actttaaagc aaatgatcgt cttattcaaa gaatatcctt
    23821 cattattgag acgacctatt ttgattgatg aaaaaagaat actaatcgga tttaatgaag
    23881 atgagatccg gtcatttgtc cctcgtgaaa ttcgatgtgt tgccagaaat ctgatctatt
    23941 tacaaaacaa taacatctta aattcatagg gagggagcct tcgtggaagt gaaagtctat
    24001 ggatcagcgc actgtgtaac aagtctgaag gcgaaacaat ggttagaaca ccatcaattg
    24061 ccttatcaat ttgttgactt agaagaacgc gatctaagta cagaagaagc agaagaatta
    24121 tgcagttttg aagaagtgca agcagaacag ctatttgcga cttggtctga ggcttttcac
    24181 aacattgcga ttgacctttc ctgtgtagga aaaaaacaac tgattttttt atgcacacaa
    24241 cataccaatc tcctcagacg tcctttgatt attatcgatg aagttctatt tattggatac
    24301 aatgagcagt tgatggaaca gcttatctta aataaataga ctttaactgt tttaatcaag
    24361 tcactgagca tttcatacag ttgtaacagt cgaacactta gcatcaagcg tgttatgtgt
    24421 tctttttttg actttatttt tctactatag agacatgctc aatttatgtg gtcaaaattc
    24481 aaatctaggt ggggatatag agttaaaaat atattgacat gtgtaaattt acatgatata
    24541 ttcatttctg ttgttttaag tagcaaagca ataaaatagt attttaaaaa acatataaaa
    24601 tagttgttga catgttagaa atgacatgat aagataactg agttgttaaa aaagaaccac
    24661 ttgtgaagct tgattctatc gaaaaaaact tcaaaaaagt tcttgacaaa taacaaatga
    24721 taagttatga taaatgagtt gctgtaagat ttcgaaaaac gaaatcaaaa aaacttttaa
    24781 aaaagagttg acaattactt gtctctctga tatgatataa aagttgctac ggcagccaaa
    24841 aacgaatcgg ggaacactcc tgatgaagta gacctttgaa aactgaacaa agtaagacaa
    24901 accaaatgtg tagggcgtct tgattcaatt caaga
//
LOCUS       DACCKU010000038        24855 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.040, whole genome shotgun sequence.
ACCESSION   DACCKU010000038 DACCKU010000000
VERSION     DACCKU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.040"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            123..1382
                     /locus_tag="IVN13_001457"
     CDS             123..1382
                     /locus_tag="IVN13_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP7011953.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGDFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(1470..2417)
                     /locus_tag="IVN13_001458"
     CDS             complement(1470..2417)
                     /locus_tag="IVN13_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7011954.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDHRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            2811..4103
                     /locus_tag="IVN13_001459"
     CDS             2811..4103
                     /locus_tag="IVN13_001459"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP7011955.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            4118..5887
                     /gene="aspS"
                     /locus_tag="IVN13_001460"
     CDS             4118..5887
                     /gene="aspS"
                     /locus_tag="IVN13_001460"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP7011956.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            5996..6871
                     /locus_tag="IVN13_001461"
     CDS             5996..6871
                     /locus_tag="IVN13_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7011957.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            7137..9020
                     /locus_tag="IVN13_001462"
     CDS             7137..9020
                     /locus_tag="IVN13_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP7011958.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(9095..9379)
                     /locus_tag="IVN13_001463"
     CDS             complement(9095..9379)
                     /locus_tag="IVN13_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP7011959.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(9369..10103)
                     /locus_tag="IVN13_001464"
     CDS             complement(9369..10103)
                     /locus_tag="IVN13_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP7011960.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            10232..10855
                     /locus_tag="IVN13_001465"
     CDS             10232..10855
                     /locus_tag="IVN13_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP7011961.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            11047..12168
                     /locus_tag="IVN13_001466"
     CDS             11047..12168
                     /locus_tag="IVN13_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP7011962.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            12175..15303
                     /locus_tag="IVN13_001467"
     CDS             12175..15303
                     /locus_tag="IVN13_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP7011963.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            15322..18516
                     /locus_tag="IVN13_001468"
     CDS             15322..18516
                     /locus_tag="IVN13_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7011964.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(18662..19369)
                     /locus_tag="IVN13_001469"
     CDS             complement(18662..19369)
                     /locus_tag="IVN13_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7011965.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            20134..20532
                     /gene="spxA"
                     /locus_tag="IVN13_001470"
     CDS             20134..20532
                     /gene="spxA"
                     /locus_tag="IVN13_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP7011966.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            20864..21517
                     /locus_tag="IVN13_001471"
     CDS             20864..21517
                     /locus_tag="IVN13_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP7011967.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            21743..22741
                     /locus_tag="IVN13_001472"
     CDS             21743..22741
                     /locus_tag="IVN13_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP7011968.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            22917..24725
                     /gene="pepF"
                     /locus_tag="IVN13_001473"
     CDS             22917..24725
                     /gene="pepF"
                     /locus_tag="IVN13_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP7011969.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT         8352 a         4259 c         5212 g         7032 t
ORIGIN      
        1 tagactgaaa aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca
       61 gcagtcggaa aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga
      121 agatgaaaga agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa
      181 aaggatacaa catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa
      241 agattttcaa taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg
      301 atccaaggaa tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg
      361 gtcaaggtgg agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta
      421 tggatctaat gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga
      481 cacaagcact tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt
      541 tctttacatc tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg
      601 tcgaaaaatt agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg
      661 atcacataaa aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg
      721 aagaagctcg acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca
      781 atgccacaga tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga
      841 acatcatccg tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt
      901 tcagtagacc agatttgtat cagcaattaa aaaatgactt ttcgacgttg caagatccgt
      961 ttattcaaga aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga
     1021 aagaagtaga agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat
     1081 atggaacaga ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt
     1141 atgtagggaa agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg
     1201 ggggagattt tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac
     1261 gtgtgttttc cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga
     1321 cgattccatt caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt
     1381 agattagcat aaacttatcg aaaaaaggaa gatcaaacag tgctgatctt ccttttttca
     1441 tattactata aattgataag gtcaattgat caaaatccac gaaatgcttc atcaatggtt
     1501 tgataatctt cattggataa atgcaaatct aaagcttgga cattgtcaga tacttgctct
     1561 ggacgcttag ctccaggtat aactacagac actcgtggat tcttcatgta ccaagctaaa
     1621 acaagttgcg caggagtggc ttggtatctt ttagcaatgg ggcgaagtac atcgacagcg
     1681 gttaaaattt ctctgaaacg ttctccttgg aaatctggat ttctttttcg atgatctcct
     1741 tcaccaaatt gtttttcctc accaagttca tattttcctg tcagcaaacc ggaagcaaga
     1801 ggaaaatagg gaacaaaact gattttgttt ttttcaagat aagggaataa ttctttttca
     1861 gcctgccgat ggatcaaact gtatttatct tctactacat cgacatatcc atctgcattc
     1921 gcttctttca attgttctaa cgtgaagttt gacacaccga ctgctcggat ttttcctgct
     1981 tcttttaatt catgcaatgt agcaactgat tcattttttg gtgtgctttc atccggaaag
     2041 tggatataaa aaatgtcgat gtaatcagtt tgcagacgtt ttagtgcatc ttccaccgat
     2101 tgtttgagaa actcaggaga attatcaaat gttcgccctt tatttggtac atgagcagct
     2161 tttgtagcga tgacgacacg tgagcgatca tattcttttt ccttcagtac ctcgccaatc
     2221 agttcttcgg aacggccgtt tccgtaagca taagcggtat cgatcaaatt gatcccgcta
     2281 ttcaaagctg tacgaactac ttgttttcct gtctcatctt ccaaaccgtc aaaaagatta
     2341 tgtcctccaa cggcatttgc tcctaatcca agctgttctg cgtagacttg tgaatgtccg
     2401 attcttattt ctgccatagc tgattcctcc tttttgtcta gtctaaaaca aaataccaaa
     2461 aagctgaact aaaacgtttt tgtgagggga aatagtcaga tatctcttga ctttgagact
     2521 ggtttttagt aggttataag taatatcgaa tgtcaatgaa agagcgagta agtttttgaa
     2581 actttacaag agaggatcat cgaggctgaa aatgatctaa agtttcatta gcctaaagac
     2641 actctggaga tggacgagtg aaaagccgtc ccgcgtacga gcgttattcg taagaggaaa
     2701 gagaaatctt tcaaaattag gtggtaccgt gtatgaattt cattcaccct tgttcatgat
     2761 gaacaagggt gattttttta tttacatagc taagaaaggg agagaaaact atgagtttcc
     2821 aaagaccaaa aggaacaaat gatattttgc caggcgaatc agaaaaatgg cagtttgtcg
     2881 aagaaacagc acgtttatta ttcagagatt atcagtataa tgaaatgcgt acaccgattt
     2941 ttgaacacta tgaagtgata tcccgaagtg taggggatac aacggatatc gtttcaaaag
     3001 agatgtatga tttttatgac aaaggggaac gccatgtgac gcttcgacca gaaggtacag
     3061 caccaatcgt tcgttctttt gttgagcata aattgttcgg cccagagtat gcgaaaccgt
     3121 tcaaaacttt ttatatggga ccaatgtttc gctacgaacg ccctcaaaaa ggacggttac
     3181 gtcaattcca tcaaatcgga gtagaagcat ttggatcaga aaaccctgca actgatgtgg
     3241 agagcatggt gatggcactt gatttcttca aacagttggg gatcacgcaa atccggctag
     3301 tcatcaattc attaggtgat aaagaaacgc gtcaagccta tcgtcaagca ttgatcgact
     3361 atcttttacc ttttgaatca gagctgagtg aagactctaa acgccgcctt catgaaaatc
     3421 cattacgcgt attggacagt aaagacaaac gagatcaaaa atttgtggca gaagctccat
     3481 ctatcttaga ttatctaagc gagcctgctc aaaaacattt tgataccgtt gtttccatgc
     3541 tggatgcatt ggctgttcct tatgaaatcg acagtaatat ggttcgcgga ctagattatt
     3601 acacacatac gatttttgaa atcatgagtg acgcaccaaa aatgggggca caagcaacga
     3661 tttgcgcagg cggacgttat gacaatctag tggaagaact aggtggtcct tcaacacctg
     3721 gcttcggatt tgcaatgggt atcgagcgat tactcttgac tatggaggca gaggaagtag
     3781 ttatccctgc atttaatgag ttggatgctt acgtggtcgc actcggagac gaaacaaata
     3841 tcgaagcatt gaaagtggtc caagcgattc gaaactttgg tttttcagct gaccgcgact
     3901 tcatgaaccg taaagcaaaa gcgcaattta agacagcaga taaagcgaat gccaaattag
     3961 tattggtgat cggtgaagat gagcttgcta gtcaaacgat taatgtcaaa gcaatggcaa
     4021 gccgaaaaga aaaaagtttt ccattagaag agatatatga acattttgat gaagtctatg
     4081 atgaaatgac tttattcgag taggaggaaa aaaataaatg gcacaaagaa cgatctattg
     4141 cggattagtt tccgaacaac aattagaaca aacagttaca ttaaaaggat gggtacaaaa
     4201 aagacgagac cttgggggag taatctttat cgacctacgt gatcgtgaag gaatcgttca
     4261 aatcgtcttt aatcctgaaa tcaacaaaga agcatgggaa atcgcggata aatgtcgcag
     4321 tgaatacgtg atcgaagtga ctggggaagt tagaagacgc ggagaaaatg cagtaaatcc
     4381 taaaatggcc actggtgaat tcgaagtgat tgcaagtgaa atcgtgattt tgaatcaagc
     4441 aaaaaccccg ccatttcaaa ttgaagatga tcaagcaatc agtgacgaaa tacgtttgaa
     4501 atatcgttat ttggatctgc gtcgtccaaa aatgactaaa aacatcaaat taagaaatgc
     4561 gacaacaaaa gcgattcgtc attatttaga tgataatgac tttctcgata tcgaaacacc
     4621 ttacctaggg aaatctacac cagaaggcgc aagagactac ctagttcctt cacgtgtcca
     4681 tgctggacat ttctatgcgt tgccgcaatc tcctcaattg tttaaacaat tgctgatgaa
     4741 tgctggattc gaccgttatt atcaaatcgt tcgttgtttc cgcgatgaag atttacgtgg
     4801 agaccgtcag cctgaattta cgcaagtaga tattgaaaca acgttcttaa cagcagaaga
     4861 aatccaaact tacacagagg gattgattgc gaaggtaatg aaagaagttc gcggtatcga
     4921 agtcacattg ccatttcctc gtatgaccta tgatgaagca atggctcgct acggcagcga
     4981 caaaccggat acacgttttg atatggaatt gatcgatcta agcgatacag tcaaagaagt
     5041 agaattcaaa gtcttccaaa tggctcttga aaacggcggt gtcgtgaaag cattgaatgc
     5101 taaaggcgct gcagatcgct actcacgaaa agacatggat cagttaggtc aatatgtagg
     5161 acaattcgga gctaaggggc ttgcatggct gaaagtagaa gaagacggat tgaaaggtcc
     5221 aattgctaaa ttcatgggag aagcaacaga agcaatcatt aaagcaacag atgcaaaacc
     5281 aggtgacttg ctgatgtttg gcgcagataa aagtgaaatc gtcgctgctg cattaggtgc
     5341 tatccggaca cgtttaggaa aagaattagg actaattgat gaatcgaaat tcaatttctt
     5401 atgggtaact gattggccac aatttgaatt ttcagaagaa gaaggtcgat atgtctctgc
     5461 tcaccatcca ttcacgatgc caaaagaaga agatatcccg ttattggctg aagaccctgc
     5521 aaaagtctat gcagaagctt atgatatcgt cttgaacggt tatgaactag gcggaggttc
     5581 tatccgtatt catactcgtg agcttcaaga aaaagtacta gaaactctag gctttacaaa
     5641 agaacaaatg gaagaacaat ttggtttctt gttaacagct ttagattacg gattccctcc
     5701 acatggcggt attgcattag gattggaccg ttttgcaatg ttattagccg gagaagataa
     5761 tattcgtgaa gtcattgcct ttcctaaaaa cggaaaagca gcagatgtga tgagtgatgc
     5821 accaagtatg gtttcgcctt tacaattgtt cgaattgaat attgatgtaa cgaatataga
     5881 agaatagccg ataaaaaggt aaaaaggatt tgtcgaatac gcttcttttg acaaccttga
     5941 tttaattgaa aaacacttta taatgaagcc ataacatata aagcggggct tttccatgaa
     6001 aaaaacattc gaaaattggg cttatcatga ttacacgacc aagctttcgg tatctatcgt
     6061 ttatgcgttg atcgcatcag tcgcattgaa tttcttttat cagccaggac atatttattc
     6121 aagtggtatt actggtctag ctcagatttt gacgactttg tcaaaatcac tgataggatt
     6181 cgaagtaccg gtatcaacaa ctttgtattt actgaatata cctttgtttg tattggcgtg
     6241 gttaaaaatc gggaagaagt ttacaatttt cacttttatc acagtcactt tgacctccct
     6301 ttgtatccat attttaccgg aaacagtcct atctaccgat ccaatcatct gtgcgttgtt
     6361 tgggggggct gtgatgggac taggtattgg gctagcgttg agaaacggcc tatcatcggg
     6421 cggtctagat atcgtgagta tcacgattcg taaaaaaaca ggaaagtcga ttggttctat
     6481 ttcgatctac ttcaatgcat tgatcgtttt tgttgccgga attctatttg gctggcaata
     6541 tatgttttac agtacgttgt ctattttcgt cagtggaaaa gtaactgatg tcgtttatac
     6601 taaacaaaag aagatgcagg taatgatcgt aacgaaacat ccagaaaaag tagtaaatga
     6661 aatccaaaac tgtatgcgta gagggatcac gatcatcaat gaagcagaag gtgcttataa
     6721 gcatgataag cagacagtat tgatgacgat cgttacacga tacgaactgc ctctacttag
     6781 agaagcaatg aaagcttctg atccacaagc ttttgtaagt attgcagaaa acgtccagat
     6841 acttggtcgt ttctatgagg atggcattta gatcgagttt gacaaaaatt catgaaatac
     6901 tgctgtagac agacgttcgt ttgtctgcag tttttttcta ccatagtaga atcagcaaag
     6961 aaaaactgct tttttatttg aaattttctt aaagttgaaa aaaagcatgt ttattttttt
     7021 aaatttatgg gatgatagaa aaaaatagta gagaaaggac acgattctac ttgctatttt
     7081 cacttaatac ccgtatatta cgaatgaaaa aagtttatga aaagttggaa tattgaatgg
     7141 aaaaacaaaa aaggttaaaa aacagccgct acatcactgg gtttgatggg ctccgttcat
     7201 tagctgtggt gggtgttatc ttctatcacc ttctgccaac tagtatgaaa ggcggctatt
     7261 tgggcgttcc gatttttttc gtcgtatctg gttatttgat cacggatctg ctgcggcagg
     7321 aatgggaaca aaatggaaaa atcaatattt ggcaatttta tgtcagaaga atgaagagac
     7381 tctatccggc acttgttttt atgcttgtga cagcttcggc ttatatcact ttgtttcagc
     7441 gagggctgtt aaacaatctt cgaggagttg tggtcagcag cttactgtat gtcaacaact
     7501 ggtggcaaat caataacggt ttatcttatt tcgatcgatt tgctaatgag tcaccgttta
     7561 cgcatatctg gtctttagca gtagaaggtc aaaactattt ggtttggccg atcttgtttg
     7621 tattgttgat ggtatttgtt cgtaaaagaa aatggatctt ttatactgtg ttaggtacat
     7681 ccttcctctc cgccatcttg atgatgatct tgtatacacc aggaggcgat ccaacccgtg
     7741 tttattacgg taccgacact cgtctgtttt ctatatggtt gggaagtgcg cttgcgtttg
     7801 tttggccaag tacgcgttta aagaaaaaca tcccgaaaca agcgaaaaat attctgaata
     7861 tcgctggagg aatctcgctt ttggcactga ttctttgctt cttcttttta gatgatcatt
     7921 acaatttcat ctattacgga ggaatgctcc tagtgagtct gttatgcgta gtgctagttg
     7981 cagtgacagc acaccccgga gcaagtttga atcgatggct gacaaatcca gtatttactt
     8041 ggattggcaa acgaagctat ggcatttatc tgtatcagtt tccagttatg attttttatg
     8101 aagcaaaggt gactgatatt gccgatcatg ttttgttgca tactttagtg gaaatcacat
     8161 tgattctaat catcagtgaa ctttcttatc gatatgtgga gcgtccgcta gcacgttttg
     8221 attatggtcg tacattccaa gtgatgaaag gatggttcag caaaccgatc atcagtaaga
     8281 gcaaaccatg ggttgttcca ggcacattac ttgtagcaat tgcactcgtt ggtttcgtta
     8341 cggcaccgaa aaacgctgta acagccgatc aaaaacaact gcaggcaaaa atcgctgaaa
     8401 gtaaaaaatt agcagaagaa accagaaaca attcagaaaa caccactagt tcggttgacc
     8461 aagcgatcct tgataagtac gagttgtcca aggcacaagg gaataaggca caaaaattag
     8521 aactaacggc atttggtgat tcagttatgt tggatgctgc aacggatcta caagaacttt
     8581 atccaaaagt agtggtagat ggagatgtcg gacgacaatt atatgccagt gtaccgtaca
     8641 ttgaaaaatt aaaggaacaa gatttgttga aagacactgt tctgatcgga ctaggaacga
     8701 atggctcatt ctcggaagct cagtttgata gtgtgatgga tgcaatcggt gatcgaaaag
     8761 tatactgggt caatgtccga gtaccgacga agcgctggca aaatgacgtg aatgcgatgc
     8821 ttgaacagat gacgaaaaaa tacgacaata tgacgctgat tgattggtac agctacagta
     8881 atgaccatag tgactggttt tatgatgatc aagtccatcc aaatcctgaa gggatgacac
     8941 attacattca cttagtcagc gaagccttgc taggaggaag ttcaagtacg aacagcagtg
     9001 agacaaacgt gacagaatga tagctacaaa aaacttcagg caaaaacatt tgtttttgcc
     9061 tgaagttttt ttatatttac cgtgttttta acattcatat agattcagga agcaaaacgg
     9121 ttgagtgtgt ctgtaaataa cgttcttttt gttttcgggt gagttttttg aatgcagctt
     9181 ctgcttttgt tgcttcgctt cttgaagaaa atacttctgc atggatcatc ttaacaggtc
     9241 ttcttttttg aagacgagta tatttagcac cagtaccatt gttatgttcc tctaatcgtc
     9301 tagaaagatc ggttgtatat ccaccataaa atgtctgatc tttacagaat aatacgtaaa
     9361 aataatgttc atttgccata gagcatctca cgaatctctg cagtgtattc tccatccata
     9421 ttgtaagtga tcaatggtgg agcgactttg aatccgtcta atttgccttc tttgattcct
     9481 tcaactaaca aaatatttgc ctcttttcca ggttttgggt aaacaaattg gatcctttta
     9541 ggtgcaattc gatagtcttc catgacctga atgatttcta agaaccgatc aggacgatgg
     9601 accatagtaa agcgtccatt tgtttttagt agcttacttg aaatatcgat cacttcttct
     9661 aacgtcgtat gaatctcatg acgggcgatt gccaggtgtt tattagggtt cgttttattt
     9721 gttggcagcc ctttgaaata aggtggatta cagacgacta agtcacaaga attatgtcga
     9781 acgacagata agctgtcttt cagatcgatg gtgtggacag tcatttgttc atttaaatgg
     9841 ttaagcgcga tactacgtct tgccatgtct gctagacgag gttgaagttc gatagcgtcg
     9901 atagccgcct gggttcgttt acttaaaaaa agtccaactg cgccatttcc tgcacaaagg
     9961 tcaacaatct tccctttttt aggtacacgg gaaaagtgag caagcagcac agcatctaaa
    10021 gaaaaagaaa acactttgga actttggata atcttgatat cttctgcgta taattggtcg
    10081 attcgttcgt cttcgtgaag catggattcc ctccaaaatg aaaaaaatag tcgtctgtat
    10141 tatactattt ttattaaaaa gaaccaaggt ggcttgtcaa acctctattt tttatgcata
    10201 ctatgaccat aagaagggaa aggaagagac tatgttttat cgatttatgc gcggggtagt
    10261 tcgagtacta ttagccttag ttaatggaaa tgcccgctat gagaataaag aagttttacc
    10321 gcaagatgag aattacatac tagttgctcc ccatcgtact tggtgggacc cattatatct
    10381 ggcggtagct gcagcaccca aggaattcag ctttatggca aaagaagaac tgttcaaaaa
    10441 tccagttctg cgatttatat taactcgatc caatgctttt ccagttaaaa gagaaaatcc
    10501 aggaccaagc gtcattaaaa cacctgtaaa gattttgaaa tcgacaaatt tgagtttgat
    10561 catgtttccc agtgggacaa gacatgctac agaacttaaa ggcggaatgg cattgatttc
    10621 cagaatgtct aaagcgaaaa tcgtgccagc tgtatatcaa ggtccgttga cacttgggga
    10681 tctgttcaag cgtcagagag tgacagtgcg tttcggcgaa ccaatcgact tatcagatat
    10741 caagaaaacg gataaagaag gtatggcgga gatcgaacga cgaacacaag cagcttttga
    10801 caaattggac aaagaagtga accctgattt caaatatgaa gtcaaaaaaa aataaaatga
    10861 taagtttatg cgtgggcggt cttttgaatc tactgtcctg ttcagttcag actttttcaa
    10921 tgaaaacgag ggagagacgt caagtctgcc ctctttttgc gttccaatat gtaaaataga
    10981 agacaggtgt catgtaatgg atcaaagtgt tataataatt agaaggacat tttttgaggt
    11041 gattgaatgc gttttttaca tacagccgat tggcatatcg gaaaaaaact acaaggttat
    11101 gacttgattg aagaccagcg tcatgtacta tcttctatac tagatatcgc gaaagaagaa
    11161 aaagtagagg caatcgtgat tgccggagac ttatatgata ggagtgtccc agctgttgat
    11221 gccgtagaat tatacaatga attgatggtc gattggaatt taaaggaaaa gatcccgatt
    11281 tttgctatat caggaaatca cgatagcagt acgagacttt cagcgggtac gccatggttc
    11341 agttattcga actatttttt gcgaacacag ctagcagaag catttgaacc agtcgagttt
    11401 aaaaacgtac aatttttctt tcttccttac tttgaaccaa ttgcagcgag attatatttt
    11461 gaagatgaca atatccgaac gatcaagcaa gcggtagcca aggtgataga taaaatgcag
    11521 gagctatttg attcagaaaa aaaacaagta ttgattagcc atttttttgt aatgggaagt
    11581 gtaaaatctg attcggaaac gaaaatcgaa gtaggcgggt tggattcgat ccctggtgaa
    11641 ctacttacac cgtttgatta tgtagcacta ggacatttgc ataatcaagc tgctttgaga
    11701 caggataatg ctcgctatag cggttcccca ttgaaattct ctttatctga gatcaaccaa
    11761 aaaaaaggcg tatggatcgt tgacctgaca acaggcctgg agttgaattt tagggagatc
    11821 gaacccctgc atgatattga agaaataaca gcgagtttca aagaattgct ggatcctgcc
    11881 ttttatcagt ccgtcaatcg ggagaattac atctatatct atctcactga tagaacgatc
    11941 atcccgaata tgatgaacca gcttcggcaa atatatcctc gtattttggg gattgaacgg
    12001 gcaaacggtc gagcagaaca aacaaagcgc aaacgaaaag tactagaaat gaaagatcct
    12061 caaaaattag cagaagcatt ttttagtgaa atgacggaag agcagctaac tgcaaaccaa
    12121 gaaaaatggc tcggagaaac gttgcaagaa atcatggaaa tggaatgatc aagaatgaaa
    12181 ccgaaaaaat tacaacttaa aaattttggt ccttttatca atgagaccgt agatttttca
    12241 cgtttgacag aagcaccgct ttttttgatc agcgggaaga caggagctgg caaaacgacg
    12301 atttttgatg ggatcacctt cgcacttttt ggtgaaactt ccggaagact tcgctctgga
    12361 aaagagatgc gttctttgtt tgctacacca gacgaagaaa caagcgtgac tttttctttt
    12421 gaacatcagc agatgagcta tgaaatcgaa cgaaaaccag aacaagtttt agcaaaacga
    12481 aaaggaaacg gaacaaaaaa acaggcagcc aaagtaacgt tgaccatttt tgatgggaaa
    12541 ggaaaagaaa taaaacaaat cacaaaacgt acagaagcag atcagttgat cagagaactg
    12601 atgaatctcg atgcgaaaca gttttctcaa atcgttttac taccccaagg agaattccgt
    12661 aattttttgg tttcatcaag cagtgataaa gagactgttt tacgtcattt attcggtact
    12721 caatttttcc aacagttcaa tgagcgatta aaagaaaaag caaaacaaca gcaacaaaaa
    12781 ttggatcatt tagagcaaga gctgcatctt ctgcaaaaaa ggtttgtttt agtcaaagaa
    12841 gaggaaccag cagcagggtt tgaagccgtt ttagcccaat ggacaaacca tcaggcaata
    12901 ctcgaaaaac aaatcacgga agaaaaagaa gctctaacgg aacttcagga aaaacaaaag
    12961 caactagaaa attcctacta tcgttttgaa agcttgaaaa aaagttatga agaaaaaacg
    13021 atattaataa acaagcaaaa ggaattagct gctcaaaaag acgaaataga ggaaaagaaa
    13081 cgatggattg ggtattatga atttacgcaa cgattagtcg aaccaatcca gcgcctacaa
    13141 gaaataaaag ttgaaaaaat agaactgtca caaaaaagta gtgagcaaac aaagaaacta
    13201 gcgatgacta cgcagaatta tgaagagtgg caaaaaaatg aagcaaaccg cgtacgccga
    13261 caaacaaaaa tcaaagaagc tgctcaaaaa ctgcaaaaca aacagatgat ggtgccaatt
    13321 gtcgaatcct tacgtagtaa aaaacaagac gcagaacaat taacaatttc attggataaa
    13381 gaagaattga atgccgagaa gctgtatcaa aatctcgcta tctatcagga aagaactcga
    13441 attcttcaag aaaatttact ggatcaggaa aaactgcaag aagatcgtct tgcttttgga
    13501 cagttaaaat atctagatga aaagcagcaa gaaatagaaa aacaaaaagc aaaagacact
    13561 gggtcgctcg cacagataag agaagaactc aaaaaagcaa ctgaagaaat aacaaaaaat
    13621 agtcagctat tgaaagaaca gacagaacaa gcagaacagc tgaaaagcca atgggcaaag
    13681 cagcaaattg ctcgtcttca gctttttttg cgtccagggg agccatgtct tgtttgtgga
    13741 tcaacggaac accccataca acaattaaaa cacgaacagc caaataagga agatatctta
    13801 aaagtagaac aacaactggc agaagctgag tctgctgtag aaagaactca gcaaatcatt
    13861 gccaaaagtg aagcacatga aaataatctt caacaacaat tatcagattt agaacaaaaa
    13921 atcatggcaa cgaatataca attggctgaa caagaagaac acttgcaaca ggctttgaaa
    13981 aaggcatttc ctgagataac agaacaaaaa acaaaaacaa ctcttgtagt aatcgaacaa
    14041 tctttagata gacgaaaaag caagatggcc aatgcaagaa tagagctgga agaaataaga
    14101 caacaagcag aaaagctcga acaacaatta gctgtctaca aacagcagca gacagaaaaa
    14161 aataatcagc tccagcaact gcaaggagaa atcaaaagtt tagaacaaca attgcaagat
    14221 acggatagta actgtttaga agaggagata cgacgacttg aaaaacagtt agaagaagac
    14281 tcgaatcaac tagctgttga gcagaaagca ggagtgcaat tgttgcaaga tcttcatacg
    14341 cttcggacac agcttgatct tcaacatgaa caaatagaaa aaatagatga aaaaatagaa
    14401 aaggaacaag aaagaatagc taaaaagtta gaaagccagt cgtatttttc aacaattgaa
    14461 gaaatctgtg catttgcaca acagcaagca caatataaag aaaaccaacg tacagttact
    14521 tcttatcatg aagagcaatt agtgacagct gaccggctgc gtcaattaga aaatatccag
    14581 ctaactgaga cattcccaga tacagaagag atcagccaac agttagaaga aataagacgt
    14641 atgatcgaaa taaagcaaga aaagatatac ggtttacaag aacaaaaaat tagtaatcaa
    14701 aaaatctacg atgaatgtct ttcgatttat cacacaagcc agacgaaatt agatgagctt
    14761 acgcaattac agcagctttc gcaaacattc aacggagaaa atcctaagaa gactagtttg
    14821 gaacggtatg tgcttcaggt ttatcttcaa gaagtgttac aagtagcaaa tgatcatctc
    14881 caacgtttaa cgaaaaatcg ttatcaattt gagctagcgg ataccattgg cagttatcga
    14941 gggaaaaccg gattagaaat caatatctat gatgatcagg caggtatgtc tcgaagtgcg
    15001 cataccctgt ctggcggtga gagttttatt gcagcacttt ctttagcatt agctcttgcc
    15061 gatgtgatcc agacacaagc tggtggtgta gcaatcgatg cattgtttat tgatgaagga
    15121 tttgggtcat tagatgaaga agctttggaa atggcaatgg aagctttaga aacgatcgaa
    15181 agtgaaggaa gaatgatcgg catcattagt catgtaagag aattgaaaga acgagtatta
    15241 caacaaataa gaattgatac agaaggtagc ggacaaagca aaatacgcta tcaacttgga
    15301 taaaaaggga gattggtaac tatgaaatgg agtattccag aaaaagtcat tgcacgtgga
    15361 cgtcaatact tgcaagaagg ccgagtgtta tcggttgtcc ctgaccaaga agaaaacgtg
    15421 tggcatgcag aagtcttggg cagtgaattg tatatggttg atttggatgg tacagcaaaa
    15481 gagatagatt actgccaatg tccctattgg gaggaacata aatattgtaa gcatacagtt
    15541 gctgtggaac tttatttacg gtcgaaaaat ctatctcgta tcatgaaaaa agaccagcca
    15601 atgctagaac cggtaaaagc aacaagtgag ggagaaatca ttgcaaaagg tttttctcgt
    15661 ctaaaaacac aaaacaatcc tcaagcggtg caacctttga ttattgaata ccaagtagaa
    15721 acgattgaga ctaatcagta tcacccagag ctttctattt tggcacttta cttgcgtatc
    15781 ggttcattgg catcaccgaa aaaaacatat atcgtaaaaa acatctatga ttttttacaa
    15841 gcttacacag aaaaagaaac ttacactgtt aataaacagt accagtttcg tctagataaa
    15901 cgggcgtttc aaaaagaaga tcaaaaaata ctcacgcatc ttgcaggaag tgctcagacg
    15961 catcaattat taggtacgaa cggcttacaa gtcaaaggaa aactagataa aagatatctg
    16021 cttctcccag tagaacaagc ccgcttcctg ttagagcaga tgaatcagac tacacgtttg
    16081 catctgcaga taaatgaaca aaacgatcaa ggaatacaat tttccgatca aaacaaacct
    16141 ttatcattca aagtggaaaa atttggagaa gattatttgc tgcaagcatt gaatgatttt
    16201 gattttttct ttatgcatta tcagtgggga gttatccaag ggacatttta ttctctgacg
    16261 aaatatcaac aaacggttta tcaaacttgg aaacaattga ttcgccgatt ggagaaacca
    16321 gaagtagtgt tcaagaaaaa agaattaccg tctttgttta aagagattat ccctctgctg
    16381 tcagagatcg gcgacgtagc tattgcccct gcagtagaag aggaagtagc agatatcccc
    16441 gtcgaattta ttttcttttt ccgaaaagca aaaggaaaaa tccaagctcg agtcgatttc
    16501 gtctatggcg aagtcatcta ttcgactgac ccgaagcatg aggtctcgac agaacaggct
    16561 tttcaggtgt tacgtgacac agtagaagag aaacgagtac ttgatctatt cagaatgtat
    16621 cgctaccaga aaaatgaaac tggttatgaa cgtccattgc caagcgggga agaactatat
    16681 gcctttttcc gcacagaact atctgtcttc cgtcagtttg gtgaagtcag acttggaaga
    16741 aaattgcgag agttgtattt agatgcgcat aaacatcaac cgaaaatcga agtaaccgag
    16801 accggttcat ggctcgatat tcgattcgat gtgacaggga tagaggagca agaaatcgat
    16861 catgtcctgc aaagcttgct gagaaatgat gctttttata cattagaaaa tggtcaggtt
    16921 ctttcttttg attcagaaga atttcaacaa acaagccaag tgttgcaaca attacgggaa
    16981 tcgatacgta cagaagaagg aacgatccat gtacctaaaa atcaaggatt gatcattcaa
    17041 aatcaattgg aaaaaagcaa tgctactttt agtgaatcgt tccaaacgat ggtacaagat
    17101 ctcattcatc cagaaagata ccaggcacag cttcccaaag gattgaatgc aacgatgaga
    17161 gattatcaaa agcaaggttt tcgatggttg aagatgttag gtcattatca gtttggcggt
    17221 attttagccg atgaaatggg acttgggaaa acactacaga caattgcctt tttactttcg
    17281 gaaaaagaag aacggaaaag cttctctgct ttgatcgtag cgcctgcgag cttaatttac
    17341 aactggcagg cagaggtgag aaaattcgcc ccttctttaa gtatccaagt aatcaatggg
    17401 aataaaaaag aacgtgaaga attactggct aaagacacag atatccgagt gacttcttat
    17461 gccagcttaa gacaggatct agctaattat cagtcgcaaa aaattgatta tttgattctt
    17521 gatgaggcac aaatggtcaa aaatagcagt acgaaaactg cacaagcatt gagagaatta
    17581 gctgtacctc agcggtttgc actgagtggg acaccaatcg agaataattt agaagaattg
    17641 tggtcattgt ttgcaacgat tatgccagga tttttcccga ctaaaacaaa attcagggaa
    17701 cttgccgcag aagaaattgc gcaaatgatt cgaccgttta ttttacggcg agataaacaa
    17761 actgtgttga aagacttgcc ggaaaaaaca gagatgaatc tatattctgc acttacagaa
    17821 gaacaaaaaa ctgtttattt agcttacttg cgtcaaatgc gtgaagaaat cacatcaatg
    17881 gattctgaag cattcaagaa aaaccggatc gggatacttg ctggtctgac aagactgcga
    17941 caaatttgct gcgatcctcg tctgtttatt gaagattacc aaggcggttc cggcaagctc
    18001 gaacaagtaa aagatctact ggtagctgcc aaagagaaca aacgaagagt tttgctgttt
    18061 tctcaattta cagggatgct gactatcttg caagaagagt tagccgagct agggatctcg
    18121 acattttatt taagaggcag tacgaaacca caagatcggt taagtatggt agacgcattt
    18181 aatgctggcg aaaaagatgt ttttctgatt tccttaaagg ctgggggaac tggattgaat
    18241 ctcacgggag cagatacggt catcttgtat gacctttggt ggaatcctgc cattgaagaa
    18301 caagctgctg gacgtgcaca tcggatcgga caaaaaaatg tggtagaagt gtggcgtatg
    18361 atctctgaag gaacgattga ggaacgcatg gattcactcc agcaagaaaa acgcgaactg
    18421 ttccaaaaag tgatccaagg taatgaagaa cagttgacga aactgactga agaagacatc
    18481 cgcatgattt taagtgtagg agaaatcgag gagtaagaga ctcgaaaaag cgttcagtaa
    18541 tgggtaagaa agcctttcta aagaagcgat tgtttatttg atttaaaaaa tagcaaggag
    18601 gctgggattc cattttatcc cagcttcctt gctatcattt ttttatttct atttttaaag
    18661 gttatacaaa gattgtcttc attgtctcta acagctgatc aaaagaagaa atctcgatat
    18721 cgcaaattgg agtctgttca attttgcgtt ctcgatggtt gaaccaaagc gcttgccagt
    18781 tagcactttt tgcacctaga acatcattgt cgtaattatc gccaacgtat aaggtctctt
    18841 ctggaagcat atgaaattct ttctcagcta gctgaaaaat ctctttttct ggtttttgga
    18901 aacctgttgc ttgagaaatg agcatattgc gggaaggaat ccaattgttc aattgaagtt
    18961 gcttcagttt ttttgtctga tggtcagtcg gcccatttgt gataattcct aatgggacat
    19021 tcttttcctt caagaaatcg agggtttttt tgacctcctc atgcaagcag atattatcaa
    19081 gttcttcctc gtaaattttt tgaaataaga ggccatcttc ttccgtaata tgagggtagt
    19141 ctaaatcttt tagtgattgg ctgatccgat gtgctcgcat atattctagt gtccaatctc
    19201 ctgccattac ttttgggaag ttttcatcac tatgatggcg gaaccggata tatagtggat
    19261 gcatatctgc atctgaaaca agcggaacga ctcttttgac cgcgtttcga aatggctgct
    19321 gctgatcata catcgtatca tcaacatcaa acacaactga tttaatcaaa attttacacg
    19381 cctttcggtt ttcattgatt ttttcacaaa tccttgacta ttgtaacaga aaaaattgtc
    19441 taaaaaagct gataaataaa ggtttttctt actatatttc tatttccttg acagaataag
    19501 gtttttatat ttcagttttt ttcactaaaa atcgtgaaac acgtcaataa gtttgatgaa
    19561 caaattaatc attatggtag aagtcgattg atttatttta atgcaatctc tgtggaattt
    19621 tcgaaagatt tattttttta atacagtctc aatataacta ttcaaacata aaataacgtt
    19681 atttgattta tttgtttata gtaaatttta tttagtaatc attgctttaa aatactacgc
    19741 ttatataaca gagaaaataa gcgttataat aaagaacagt attatttaaa atgacagaaa
    19801 acattactat taaagaaata aaaaacataa aaaatcttaa gaaatcggtt tcatttagct
    19861 tttgagataa aataatagta cagttttata cgctgtttca tatgtttaga aaatttgaat
    19921 tagatttgaa aactctaatt tttaaatgat aaaaaaagcg agaatgctgg ttttactgta
    19981 tattaacctt agataacttt cattttttta aaaaaattat gaaaaaaagg tagctattta
    20041 aaagttgatt atgtataata gaaacagatg ggagattatt atgatcttct gttacaaaaa
    20101 agtgattctt atcacgaaaa ggagtggtgc acgttgttaa cattatatac ttccccaagt
    20161 tgtacttcct gcagaaaggc tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa
    20221 cgtaatattt tttcagaacc attgaacagt tctgaattaa aagcgatttt acaaatgaca
    20281 gaagacggaa cagaagaaat catctcgaca cgttccaaag tattccaaaa actgaacatg
    20341 gatttagatg atctgccgtt acaagaatta ttggaacttg tgcaaaacaa tcctggattg
    20401 cttcgccgtc caatcatgat tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa
    20461 attcgtcggt tcctaccgcg cgatgtacga caattagaat tacgtcaagc acaattgatg
    20521 gctggattat agaaggataa tacgaaactt ggaaaagact attacaaaga tagtttttcc
    20581 aagttttttt gtttttacaa aaagtattct tgattagatg tataaaaagg tagtagaaga
    20641 tgatgattgc acgaaaaata gagagggaaa attagctgaa aagtcggcta tatagtattc
    20701 gtctgccaaa aatgttatag ttaaataaga tagcttgaaa ggttgaaaaa tccgctggtt
    20761 ttattaaatt cgcttaacag cgagattttt tttctttaca tttaagctga tttcgctaca
    20821 atgaatctat attaaacatt gtggcaaagt gaggtgtagc aaaatggaaa tggaacatat
    20881 taatgaaaat acgatccgtg tactaattgg tcatgaagat ttagaggaac ggggcgtttc
    20941 atttttggat ttacttggca atcacaaaga aatcgaaaat ttcttttata gtattttgga
    21001 agaagttgat atcgaagagg agttccgagg aagcgaagct gtcactttcc aagttttgcc
    21061 aaaaggagac ggattagaac tgtttatcag taaaaatctt ccggatgaag aaatttctca
    21121 atcagaagat tctactgttg attcagaaga tgtgagtgac tttcttcgtc agcagattat
    21181 tggcagcgat ccggaggaag aattatctgg aagcgcccta gaagaacagg aaagacaagc
    21241 aacttttgct tttgaagatt ttgaacaagt gatccaactt gcaacagatg tcgaactaga
    21301 gtctgcttgg actgatttgt atttattcga agagcgctat tacctagtcg ttcacttctg
    21361 gatggaaaat ctcaaccaag cggatgtaga gaatcagatt gctcgaattt tggagtttgc
    21421 tgaaaagagc gatcgtacac cagaaacatt gaatgaatat ggacaatgtc tgatggcgcg
    21481 taatgctatc gagcgaacga aattttattt tgtataaatc agcattatag taagagactg
    21541 tgacaaaaat tgtgtcgcag tctttttttc gtataaatgt gttcaaaagc ttgcatctgc
    21601 ggtaacaagc ccaaccaaac gaaaaatatt gggaactatt tttgtttagc tgttcttatt
    21661 acttgatgca gaatagcttt ttcacgccct cttttttcgt gtaaataaat tcttagacgt
    21721 aaataagttc agagggagaa aaatgctgct tgcaatgaat gaatataata agtgctgttt
    21781 tgctaaacat gcagaaaaag gaaagaaata ttattgtcgt ggctgtcgaa aaagagtgat
    21841 tttgaaaaag ggaaagaaaa aatgtgctca ttttgcccat caaaaatcag ataactgttc
    21901 ggtctttagt gaaagagaat caaaagagca cttacagtta aaggaatgct tcatggattg
    21961 gctgggacag tcagtagaac cagtattttt ggaagcctat ttgccgaggt tgcggcaaag
    22021 gccggatatt ttgttggcga atctagcaat agaaatccaa tgttctagac tgtctcacca
    22081 gagatttatt gaaagaaccc aaaattattt gaataacagt taccaagtat ggtggatact
    22141 tggtaattct tttcttggac aatcgcaatt ctcattgata gaaaaaagtt gctgctacta
    22201 taatagaaaa agaggagtgc attgctggaa agctgatctc aagaaacaaa agctttattt
    22261 gtatcatcat attacagaga ctgtatcagg gcatatttcc ttttgcagta gctgctggac
    22321 tttttctagt agggacctta aagaaatatt cactagtaac gaaattaaaa tgaaccaaat
    22381 gaaaaaaata gaaaaagtga gtgaagatgg gaagaaatgg ctgacacgcc aacttattca
    22441 caagcaaaag aatacagtat ccatacaaga acaatgctac ttgcgtcata aacatctttt
    22501 gcatctatca tcgtggatct atcaaaaaag tcggtttttc ttttatttac aagaacaggt
    22561 ttttctttat cgaatgttgt acgaagaagc cttggaaaac cagaaagttc ctgatttcaa
    22621 ctcatggctt tgccaaataa aagaacataa aagggaatgg ctttttccta tgattgatga
    22681 agagatggtc tatcagcagt tttttaacga atgcatacac ttgtcttctt tgaaaaaata
    22741 gtaagtataa aaataggcta ggaatcggtt ttctatgtaa aacaagattt agttatgcta
    22801 aaattgatgg aagcagacac atgaatgagt agaaaagtta gatgctgtga catgtggatg
    22861 ttagactatg atctattttg atagatagag ctaagggaag aaaaggagtg gatttcatgg
    22921 aagtaaagca gttgccaaaa cgagaagaat tgcctgaaaa tttaacttgg gacttgacca
    22981 agatcttttc aagcgaccaa gagtttgatg agaaatattt ggaattatca gaagagttaa
    23041 aacaatctga aaaacacaaa ggaacacttg atcaaggcgc ttctcaattt ttaaatgcga
    23101 ttgaattcgt attgagggtt tatcgccaaa ctgaagtcat ttatgtatat gcgcacctta
    23161 aaaacgatca agacactgga aatacagatt accaagcgct ttatgcaaga gcaagcagtc
    23221 tgttttcgaa agttagtgaa gccgtttcct ggtttgaacc agaaatattg caattgtcag
    23281 atgaccagat ttggcaatat ttcaaagaag aaccaaaatt ggaagtctat cgccattata
    23341 tccagcaaat agtagataat cgagcccatg tcttatctgc tgagcaggaa tctcttcttg
    23401 ctggagcagg tgaaatcttt gatgcttcaa gtgatacatt tgctgttttg aataatgcag
    23461 atctagtttt tccaacgatt gaaggagaaa atggtgaaat agtccaatta tctcatggcg
    23521 tgtatggtca gttgctagaa agcacggatc gaagggtgcg cgaagcagca tttaaggggt
    23581 tgtacagtgt ttacgaacaa tttagaaata catttgcttc tactttaggc acacatataa
    23641 aaggacataa ttttaaagcg aaagtccgta attacagctc tgccagagaa gcgtctttga
    23701 gcaataatca tattcctgaa agtgtatacg atactttggt agacgtggta aacaagcatt
    23761 tgcctttgtt acatcgatac atggaattac ggaaacgttt attagaagtg gaaaaactgc
    23821 acatgtatga tctttataca ccggtcttag gggaagctcc aattaccttt acgtacgaag
    23881 aagcaaaaga aaaagcttta gaagcactga aaccaatggg tgaagaatac atggccatcg
    23941 tagaaaaagc attctctgaa cgttggatcg atgttgtcga aaataaaggg aaacgaagcg
    24001 gtgcttattc ttcgggaagc tatgacacaa atccatatat tttattgaat tggcatgata
    24061 cgctggatca gctatttacg cttgtccacg aaatgggaca tagtgttcat agttatttca
    24121 ctcgttcgaa ccagccttat gtgtacggcg actactccat ctttttagca gaaattgctt
    24181 cgacaacgaa tgaaaatatc ctaacggagt atttattgga aacagaaaaa gaccctcgtg
    24241 tacgggctta tgtactcaac cattacttgg atgggtttaa aggaacagtt ttccgccaga
    24301 cacaattcgc tgaattcgag catttcatgc atacggaaga tgaaaaaggt gtgccattga
    24361 ctagtgaata cctaagtgat agttatggta aattgaatgc aaaatattat ggtccagcag
    24421 tcgaagaaga cccagaaatt aaattcgaat ggtcacggat accgcatttt tattataatt
    24481 actatgtttt ccaatactcg actggctttt ctgctgcttc agcactggcg aagaaaatac
    24541 taaaccagga accagaggca ctagagaact acttggctta cctaaaagca ggtaacagcg
    24601 attatcctgt ggaagtaatg aaaaaagcag gagttgatat gacacaggct gcatatattg
    24661 aagatgcaat gtcgatgttt gaacaacgtc taaatgaatt agaagaattg atcgatcgtt
    24721 tataaggctt tctatttttg attttctcga atagaaacga aaaaaatacg tcggctctct
    24781 tgtaagggag tcgacgtatt tttttaaggc tctatacttt ttgatgttgg tgaacttcac
    24841 caacatcttt tttgt
//
LOCUS       DACCKU010000039        24725 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.041, whole genome shotgun sequence.
ACCESSION   DACCKU010000039 DACCKU010000000
VERSION     DACCKU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24725
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.041"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            161..724
                     /locus_tag="IVN13_001474"
     CDS             161..724
                     /locus_tag="IVN13_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011970.1"
                     /translation="MIKKTQNKIQRICQKHFVSRLNSNNETTSTTSSTQLIDPTEKEI
                     TAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVAVNGSMSAD
                     KTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNTIDSVTPNA
                     DKSYSFTPSTWGGLPAGGGMSTFSIGI"
     gene            926..2050
                     /locus_tag="IVN13_001475"
     CDS             926..2050
                     /locus_tag="IVN13_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011971.1"
                     /translation="MGDKVVITPTAVTGYSFTQTNGSLTALASNVNTVTYTGKQAPIT
                     IKYQDYFGQTIAPPKMMNITYGSAAQDLTTNAPTINGYTFTAVSATETKNQSAMSVSA
                     SLDTNGNVIVKDANGKQISEVILYYKTNATVSINANGSKYYDGLSVVPIVKYTFNDKT
                     ESTSLLNNDLSIDQITWNPTDFKAMNEKGVEVTEPTEIGTYTSWQLTKSGLAKLAART
                     NYLFTIVQTSDVYTIKQISGAVELGDSKTYDGKAGVPSIHVKLAEGVTSSLTPVQVAL
                     SSMDYTVDAQSAKNMVNAGSYTIKLTNSGIDKVKAANPKLSFTDIANTSGTYTIYKAN
                     AVITVDDAAFNYDAQSHSIPVGNVHVTGVLRRIIGLYPNS"
     gene            2028..2288
                     /locus_tag="IVN13_001476"
     CDS             2028..2288
                     /locus_tag="IVN13_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7011972.1"
                     /translation="MDYTLTVNSRTDIGSQIVTISLGEETVNNNYTITATDTAKLTIN
                     PTLLLPSTGGIGLLPFVFLGMIFIFSGFFYFIHRKKKAGEQR"
     gene            2285..3787
                     /locus_tag="IVN13_001477"
     CDS             2285..3787
                     /locus_tag="IVN13_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7011973.1"
                     /translation="MKKTKNVRVDIFAVLFFILLVFFIGSPTVSARESPITFTLHNRM
                     FESDKMPSISQNDGINIPDNFQEAKGLNGVTFAIYDVSDEFYKLRSEGSSVEDAQRKL
                     AQKSDSEKILAENVTKTVDGEEGIASFSASDKDEQGRDAVYRFAEIKTSDQVKEKSAP
                     FVVVLPVTNSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDKVPYLITVT
                     IPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKTKHGFVLSDSGKDL
                     SSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQFVKVDRQT
                     KQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHGLSFGSYEL
                     VEIKPPKGYILSQSTILFEVEKSSYDPLHPIPLEIINEPKANPTNNGKTPVQMQTNGP
                     SNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK"
     gene            3784..5175
                     /locus_tag="IVN13_001478"
     CDS             3784..5175
                     /locus_tag="IVN13_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7011974.1"
                     /translation="MKNIKPKIWTFLGTAEVSAAVSPTPENVTVNLHKLKFTSAPENQ
                     INNGTELTFPNSEPLNGVEFNEYDITATYYPSKDTAVPADATPFASVTTSGEGLANLT
                     LPGKSDGKDAVYVFVETPKPGVETSPNIVLSLPFYNPNSSDPDKPLDTIHLYAKNVIK
                     ESNIKIQKAGNFSGVDKLAGAQFQIKNSENQFVTGFDENTGVALYDPAESALTFTTDT
                     EGSISVNGLIDGNYTLVETKAPDGYQLTDPQKETTFIVKNGKLQQTTGIIHGADEANL
                     GNTNGVVNRENTTTDNTITVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVK
                     LMKKIANLASGEYPYAWSDEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPE
                     GYALPKNEAAYTEFTVDDDPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGA
                     VIYFKKRHQETEA"
     gene            5383..6555
                     /locus_tag="IVN13_001479"
     CDS             5383..6555
                     /locus_tag="IVN13_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7011975.1"
                     /translation="MNKSRIRILDIFMAIILVAGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGGNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(6717..7211)
                     /locus_tag="IVN13_001480"
     CDS             complement(6717..7211)
                     /locus_tag="IVN13_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011976.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            7800..8222
                     /locus_tag="IVN13_001481"
     CDS             7800..8222
                     /locus_tag="IVN13_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7011977.1"
                     /translation="MQTFLTKVSQNKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNDNNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(8388..9581)
                     /locus_tag="IVN13_001482"
     CDS             complement(8388..9581)
                     /locus_tag="IVN13_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7011978.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLILTTLTGVTALR
                     ESLQH"
     gene            9805..10182
                     /locus_tag="IVN13_001483"
     CDS             9805..10182
                     /locus_tag="IVN13_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7011979.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            10170..10508
                     /locus_tag="IVN13_001484"
     CDS             10170..10508
                     /locus_tag="IVN13_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7011980.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            10642..11085
                     /locus_tag="IVN13_001485"
     CDS             10642..11085
                     /locus_tag="IVN13_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP7011981.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSSVVNELIHRFKEAFEQ"
     gene            11334..11654
                     /locus_tag="IVN13_001486"
     CDS             11334..11654
                     /locus_tag="IVN13_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7011982.1"
                     /translation="MKRFRKCLTLVFVLSCLIQLSAFKQVVFAASEKSSVGITFEGSV
                     PQSSTDPSVPSSQDAEQSANQSRQKKYPKTNEIQQSFITWGGIFLLVISLLLIFWKRK
                     RNRE"
     gene            11651..12310
                     /locus_tag="IVN13_001487"
     CDS             11651..12310
                     /locus_tag="IVN13_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7011983.1"
                     /translation="MKKTILAAALISTTLFAGTLAVHADGLNGKSTAKIGLTAQDPDH
                     PVGPIDPIDPDDNPPSNDPTGNTGDLRIDYMSKIDFGKQTISGKTETYTAEKPALTTE
                     VQVSDLRGTGAGWNLQVSYDTEKPGFTSEDKTLNGAELTLPVGTPETVEDNSSAAPAT
                     SKVVVNNDAQNIMSAAANTGLGTWADEMDTANVSLKVPSGNLVGDYTATLVWTLSDAP
                     A"
     gene            12352..13062
                     /locus_tag="IVN13_001488"
     CDS             12352..13062
                     /locus_tag="IVN13_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7011984.1"
                     /translation="MKKQVFYTLAATVLLSSSLLSTLNVQADETEPTKDSATSTAKIT
                     FTGDNSDPVNPVDPDDPDNPVNPVDPDDPDNHGTGDNGPLSIDYVSNITFGTKDISTT
                     SQVYNAENAEPHVQITDKRGVEGGWTLTAAATKFVASDNSELKGAELLFKNGVAETTS
                     DNTSKAPVTSDLIFDNTEAKTVMTADSLKGQGTWLDVFKGTEGNNTNVQLSVPSGSAQ
                     AKEYTSTITWTLTAGPTA"
     gene            13076..14092
                     /locus_tag="IVN13_001489"
     CDS             13076..14092
                     /locus_tag="IVN13_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP7011985.1"
                     /translation="MNKLQSLFVAALSLLSIVVMPLTTYADSNESAYSVNANIPDFQK
                     NKNVSYFDLQLDPNQKKEISIHLANSAKEKAIFNVDVNNAATNSNGVIDYGQTDLKKD
                     SSAKYVLNQLVTPNNQEIELQAGESKDVTFQVQMPKDRFKGIILGGIHISKKGNVSEK
                     VDGTAIRNKYAYVIGVKLQNDTDAVTPDLKLNSAKAGLQNSYTTVFANVQNPTPGIIS
                     KVNFDAKITKKGSKDVLYETKKENMSIAPNTNFDFPISLNKEKIEAGDYTVTIDANEG
                     NTDKTWHLTTDFTIQSEKAKKINKEAVTEDKGISYLPAIITVGILLLLIIAFLSYKLL
                     KQKK"
     gene            14106..15842
                     /locus_tag="IVN13_001490"
     CDS             14106..15842
                     /locus_tag="IVN13_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7011986.1"
                     /translation="MKLNQPLNKLYVLGLLFIGAFSVIFQSTEVSAEANYPEIPEATI
                     FDGYVKASISYDKDHSPNYTAVGDTVTVTTTLEKLDDGVLPDKYKQSSVVILFSDETQ
                     GLQLKGEPEFKYELGGLEDTGKFVDPTKDLQTNVISVFNFSTKILYNDTATYDGRMKQ
                     VPISSTELDDGPYDDTYSLYKLLVAKGSKVTLSYKATVTEEALKNKQFSLHTAIYDPT
                     IGGGDFDWDHFLTTSMPSIQNLGISFDEATKDKKIDIKDDSSYQTTLTGSWAGAKDDI
                     HPVLTIDGKEVPIPDGSFKEDGTFEIPVDLKNIGNIGTNNVHIKISDSNGQVAEDDAA
                     ITLVQTLVPPQIKLSDSLANASISVFPIDQSLTIDGEWKDKDSNAVSLFYKLNGQERV
                     LQDNVSNTAKDSWLPFSQEISLADLNTGDNQVEVYAKDAEGQLSNHESFKIILNQGNV
                     SFKSINPEIQFNNMVISGTTVYSSPQATVGVVVEDTTGIPKDWQLKVKQTTSFSDGTR
                     DLPAQLTYLNGEEKLSINDSSAVDLPVVQNNATDYSLQQDANHQFRLAVSPGAYTGEY
                     QSQLEWTIERAP"
     gene            15919..17178
                     /locus_tag="IVN13_001491"
     CDS             15919..17178
                     /locus_tag="IVN13_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP7011987.1"
                     /translation="MESIMFVLIAYLNLFIWMSLLFKGASKWRFLLFYLGLMIANMFF
                     VPYTFVFFAIAYAAMCWFLYLLVENIFLSILLQNFLLNVVGISFFLAIDIPRYLGFYT
                     SYLNLSVELVLATSLFLLIRKADQKYDIFDYFSGYTKKLKGLTILSVVIYVLIYIFHL
                     GISFYSLDYILTSIITLLYSIFYTAFFIVFIIYKKKFQVIELYLKDNQETKEYYEKLD
                     DFRHDYLNYISALEYSLMNDSQENSIKLLTHLKDYSLEAIDDARHNPLKRISDPAVRG
                     LFYHFMERANQSGISYDIQVLNIINNIKADYIDVLRLLSIALNNAIEHCDETRKEEPI
                     SITINQTKERFYFKIENPADMSSTSLAKLMKRGSSTKKNGGLGLYNFSKITDKYLNAN
                     YGIKYLKRTKMFVLELTIYEKSICKTV"
     gene            complement(17231..17980)
                     /locus_tag="IVN13_001492"
     CDS             complement(17231..17980)
                     /locus_tag="IVN13_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7011988.1"
                     /translation="MKIYYIEDNLFHQELLKKELLKHKELSALSLQIYPNSELETIYK
                     RLDTLVFSSSDLFIIDIDLKTLHSGIDFAKKIRNKNPECSIIFLSNDNTRGLEIINQQ
                     IRPDRYLDKSNLEEVAQSLKQLLLEKLEAQKQSSSIIELQGATKVFLIAPDEVNYIST
                     INGSRSTLEFYGTNENFVIHDKMKNIKQKFQDYHFFNDLKSFSINPFNIKEVDKQMME
                     VHFKNKDTLLLSSSSVKKLLRHIDQLKQTNG"
     gene            complement(18100..19245)
                     /locus_tag="IVN13_001493"
     CDS             complement(18100..19245)
                     /locus_tag="IVN13_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7011989.1"
                     /translation="MDLLYKKYLIPMVLFDSSGEVCHPKISWEIQPIMEKFISPKENQ
                     SVCLFNHFPFMFAKLDFITDSKPYSLLIGPCGMIGESPDTFLFKGHSYHYGIHYTKET
                     TAAFEEFVQLTYAFLTHELLKKEELHWVYQKEIKQRSTTDQRLEDSLYDRRMQEATFD
                     SYQFELRYTEYIKRNQPEKIDWLFKKMQETYHVELSENELEGLKLKFSAFVAILTRIS
                     IDEGVPINQAFSLSDALIQGLFRIHSSSEWHTYMKEATYRFMQLIHQQPLADKSMLVK
                     QLINYIDSHIYDRVTLDELAETFDKHKTHLSALFKKEMGQTIHSYIQERKINESKHLL
                     LFTDKSYKEISVLLSFASQSHFIQVFKKTTSLTPREYRDQHYAYSVH"
     gene            19453..21657
                     /locus_tag="IVN13_001494"
     CDS             19453..21657
                     /locus_tag="IVN13_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase"
                     /protein_id="HAP7011990.1"
                     /translation="MDEKQLKQLVDSLTIKEKIAQTVQLNGDLFTDSDVMNTGPTKEL
                     GFPDDFDLSQIGSIYNINEPEKLKKIQKKIMEERNKKIPLLFMSDVIYGFRTIFPIPL
                     AQAGSYDFDLIQKAARETAKESYLSGLHVLFSPMLDLVRDPRWGRVMESPGEDVYTAK
                     EFAKHAVQGYQGDNLQLIDKYRVGACIKHFAGYGAPEAGREYNTVDMSNQRLFNEYLA
                     PYQAAVEANCLLVMTAFNVLNGVPSTGNRWLNRDILRKRFGFNGVLVSDYAAIEELQV
                     HGYAKDQADSAKKALEAGVDFDMMTSVYANSLEKLAEENKEILQLLDEAVWRILDLKN
                     KLGLFEDPYRGLEEENTGEILTEESKEIAVKLVEKSCVLLKNEDTLPLKKHQKIAVIG
                     PYGKSRLTIGFWASVSGKPQDSIPLKDGIEQHFSKEQLLFAKGFNLFDSYEPFGPLKA
                     GIEQLNGTIEDEETLFQEALETAEKSDVILLTIGEQFLESGEGASKTNLRLPEKQKRL
                     IKALAALGKPIVGILYTGRPLVLTEIEPYFSSLLLVWYPGTMGGIGIANLLAGKAAPS
                     ARLSMTFPRSEGQIPIYYAHASTGRPLETSTHSTRFVSKYIDESNEPLFAFGTGKTYG
                     KVEASWQEIQDQEDKLQLVYEVINHSAKPIETVIHIYVKDKTASIVQPVRRLIASQVV
                     SIHADEKKSINYTLSKEALMFYNNQGEKIWESGEFTFSLSTVDSEDDRTIQL"
     gene            21673..23019
                     /locus_tag="IVN13_001495"
     CDS             21673..23019
                     /locus_tag="IVN13_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7011991.1"
                     /translation="MEKLEQFLSSRLLPMSEKLQKNKVLAALMEGFIRTSPITLGIAF
                     ITIIGNFPFPGWIDFLKNVGIFPHVEAITNGATGVFSLYVVYSLAFSYAKQLGTNERN
                     ASIISLASFIMLMPQSVMTTVTQDGKAVEQAVGALRLDYLGGQGLFIGMLVALLITRF
                     YAFLAKKNIMIKLPESVPPMVTQSLSPVFVVTIIFVVVFALRVLFGLTQSGTIFQFFI
                     DVINAPLNSLVASPLSIIIIMELLAVLWFFGIHNAVLQGPLGAISMTMVVGNIAAFQE
                     GKALPYLVPSVIYMGMYAAGFMGFVTFFMIKSKSAKMRQLGKLSFVPSLFNVTEPLMF
                     GMPIILNPIFFIPQIFTQLIAGFVTWGLTTTILPISLNPTMSLLPWTTPTFVKMPLSG
                     GLNYTILMVICMAIGVVMWYPFIKIADKKELELEAALEKEEAETKAEKIINAEVEA"
     gene            23019..23933
                     /locus_tag="IVN13_001496"
     CDS             23019..23933
                     /locus_tag="IVN13_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7011992.1"
                     /translation="MKGKSMWTLSIDIGGTYIKSALIMDTQIREKRQIETPKTDKDNF
                     ILVLVELIYSYQQIEPIEFVGFSVPGAVKEASTVFFGGAVACLNEVNLKQEIEKHLPV
                     RVFVENDAKAAVLGEASFGHLKGIENGAGIILGTGVGVGLLLDGQVRKGPHCQAGEVS
                     FLIQDRGINGAESFAGINLSAVRLVKELAKLFQCEPEGPLVFDYLYQKEDEQAQTLYR
                     TYCNQVAILCFNIQCLLDLDKIIIGGGISKQKRLIRDIQKNYEAIFSVSPMIEQTITK
                     MTIEAAAFESEANLIGAAKGVRLNEISN"
     gene            23917..>24725
                     /locus_tag="IVN13_001497"
     CDS             23917..>24725
                     /locus_tag="IVN13_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7011993.1"
                     /translation="MRFPTDFLWGASTSAYQTEGAWDADGKVPSVQDMKEIPDGTSDF
                     KVAVDHYHRFEEDVRLFKELGLKAYRFSISWSRVMSNGKVNPKGLEFYKKLIALLKEN
                     EIEPIVTVFHFDLPYEIHEKGGWENRATIQAFADYCQLLFDTFGQEVNYWQTINEQNV
                     MALAGSIIGTSNKSMKEKFQENHHMLVAQALVTKNYHDGNYPGKIGPAPNIASVYAAS
                     DKPEDQLAAMNMSALRNWLFLDAAVYGVYNHNAWHILSKINACPEVTEEDK"
BASE COUNT         8340 a         4404 c         4942 g         7039 t
ORIGIN      
        1 aataaaatct ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa
       61 agccccaaac tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa
      121 aggattattt gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa
      181 taaaatccaa cgtatatgtc aaaaacattt tgtttctaga ctcaatagca acaacgagac
      241 aacctcaacc acatcctcga ctcaactgat tgatccgaca gaaaaagaaa tcacagcagc
      301 aaaagcagca ggggagaaag cttattcaga aacaggtaga agccagacat ttagtactgt
      361 tgcggccact gcgaatttca acggacatac cataccagat ggatatacag tgatatatgc
      421 caaagatgga gtcattgttg cggttaacgg atcgatgagt gcggataaaa ctgcgttaca
      481 aggcgtaacc aaagcaatcg cttttattga taaccgtcca gatatccata atctgtttat
      541 tggctccaca gtaaatggta ctactatcac attcccaaaa agtatctata tgggggtggc
      601 taccgctgga aatacgatcg attcagttac acccaatgcg gataaaagtt attcttttac
      661 accctcaaca tggggagggc ttcctgccgg aggaggtatg agtacttttt caatcggtat
      721 atagttagca aatgatattc cattcacagg gtactcgatc aaaaatgggc agatcgttga
      781 tggtaaggga aatgtttcag ttttcccaac ctatcaagca gcgacggttc aggtcaatgc
      841 gaatcggaca ggaacttttt cttatacagt caatggtgga gcagcaaaaa tcggaacgta
      901 cgggactaca ttatctggaa tccatgtggg tgacaaagtg gttatcactc caacagcagt
      961 aacgggctat tcttttacac aaacaaacgg ttctctcaca gctctagcaa gtaacgtcaa
     1021 tacggtgacc tatacaggta aacaagcacc aatcactatt aagtaccaag actattttgg
     1081 acagacgatt gctcctccta aaatgatgaa tattacttac ggaagtgccg cacaagacct
     1141 gacgactaat gcgcctacta tcaacggata tacctttaca gctgtttcag cgacagaaac
     1201 aaagaaccaa tcagcaatga gtgtttcagc tagtttagat acaaatggaa atgtaatcgt
     1261 aaaggacgca aatggaaaac agatttctga agtcatccta tattataaga caaatgcaac
     1321 agtatcaatc aatgcaaatg gcagtaagta ctatgatggt ctttctgttg taccgatcgt
     1381 taaatataca tttaatgata aaacagaatc aacatccctt ttaaacaatg atctcagtat
     1441 tgatcaaatc acatggaatc caactgattt caaagcgatg aatgaaaagg gagttgaagt
     1501 aactgagcct actgaaattg gaacctatac gtcttggcaa ttgacaaaaa gcggactagc
     1561 taaactagct gccagaacaa actatttatt tactatcgtg cagacatctg atgtctatac
     1621 gattaagcaa atcagcggtg cggtagagct cggtgacagt aagacatatg atggaaaagc
     1681 aggtgtcccg tcaatccatg tcaagctagc agagggtgtc actagttcgc ttactccagt
     1741 acaagtggca cttagcagta tggattacac agtagatgca caatcagcaa aaaatatggt
     1801 taatgctggc agctatacaa ttaaactaac gaatagcgga atagacaaag tcaaagcagc
     1861 aaatcccaag ttgtcattta ctgatatagc caacacatca ggaacttata ccatctataa
     1921 agctaacgca gtgattactg tcgatgacgc tgcctttaat tatgatgccc aaagtcactc
     1981 gattccggtt ggaaatgtcc acgttaccgg tgttcttagg cgaatcattg gattataccc
     2041 taacagttaa cagccggaca gatattggat cacaaatagt aacgattagt ttaggcgaag
     2101 aaactgtaaa caataattat acgattaccg caacagatac cgctaagctg acgatcaatc
     2161 cgactctctt gcttccttcg acaggcggga taggtctact gccctttgtc tttttaggaa
     2221 tgatctttat tttttctggc ttcttttatt ttatccatcg taagaaaaaa gcaggtgaac
     2281 agcgataaaa aaaacaaaaa atgtacgtgt ggatatattt gctgttctct tttttatcct
     2341 actggtcttt tttataggtt ctccgactgt gtcagctaga gaatcaccta ttacatttac
     2401 tttgcataat cgaatgttcg aaagcgataa gatgccaagt atcagtcaaa acgacggaat
     2461 aaatatccca gataactttc aggaagcaaa ggggttgaac ggcgttactt tcgctatata
     2521 tgatgtctcg gatgaatttt acaagctgcg gtcagaagga agtagtgtgg aagacgcaca
     2581 aagaaaacta gcacaaaaaa gtgatagtga aaaaatactg gcagaaaatg tgacaaaaac
     2641 agtagacggg gaagagggca ttgcttcttt tagtgcatcg gacaaagacg agcaaggtcg
     2701 tgacgctgtt taccgatttg cggaaataaa gacttcagat caagtaaaag aaaaatcagc
     2761 accatttgtc gttgtgcttc ctgtgacaaa ttcatctaat cataaattga cgaccatcca
     2821 cttgtatcca aagagtgaac aaaaaatacc tgctgcactc acattaacaa aaacagtaga
     2881 gaataagcag acagattttg cagatgggga taaagtccct tatctcatca ccgtaacaat
     2941 tcctgagaac atcaacgaga taggaaccta tacgatcaaa gatacagctg atcctcagtt
     3001 atggctagaa ccagaaacga tcgatttttt gattggtgga gacaagattc atacatttca
     3061 cacacaaaaa acaaaacatg gttttgtctt atcagattct ggaaaagatc ttagttcgtt
     3121 tgctgggaaa aaattaacga tccgttacca aatgacattg aaagaaaaca gtgaaaaaac
     3181 gacatttgat aacgatgtac gtttgacgac tgacaatcaa acagtagaaa cacatcttgc
     3241 catccaaaca ggaggcaaac aatttgtcaa agtggatcgg caaacgaaac aaaaactagc
     3301 agatgctcaa tttctagtca aaaaaaatga ccaatactta gcgcaagaaa acgggataaa
     3361 tcattgggta gccaaagatg attcaagagc aacgattttt acttccagta aagatggaac
     3421 attttctgtc catggattgt cttttggaag ttacgaacta gtggagatta aaccgcctaa
     3481 aggatatata ttgagtcaat caaccattct ctttgaagtt gaaaaaagca gttatgatcc
     3541 gcttcatcct ataccattgg aaatcatcaa cgaaccaaaa gcaaatccga cgaataatgg
     3601 aaaaacgcca gttcagatgc agacaaatgg tccatcaaat gaaaaaaatg gaggaagctt
     3661 tccaaaaaca agcgaagaga tgcttggagg tttctcaatt ttagggttgt tgctagtcct
     3721 ttcaacaggg acagcttggt tttataaaaa acaacaaaaa ggaaacaata ggagggaaat
     3781 aaaatgaaaa acatcaaacc aaaaatatgg acgtttttag gaactgcaga ggtttctgca
     3841 gccgtatcac ctacaccaga aaatgttacg gttaatttgc ataaattgaa attcacttca
     3901 gcaccagaaa atcaaataaa caacggaacg gaattgacat ttccgaatag cgaaccgttg
     3961 aacggcgttg aattcaacga atatgacatc actgctacgt actatccaag taaagacaca
     4021 gcagttccag cagacgctac acccttcgca agtgtaacga cttctggtga aggattagct
     4081 aacctgactc ttccaggtaa atctgatggg aaagatgctg tatacgtctt tgtagaaaca
     4141 ccaaagcccg gtgtcgaaac gtcgccaaat atcgtattaa gcttaccctt ttataacccg
     4201 aattcatcag accctgacaa acctttagac acgattcacc tatatgctaa aaatgtcatc
     4261 aaagaaagca atatcaaaat ccagaaagct gggaactttt caggggtaga taagttagca
     4321 ggggcgcaat tccagatcaa aaattcagag aatcaattcg tgaccggttt tgatgaaaac
     4381 actggtgtcg ctctctatga tcctgctgag tcagcgttga cattcacaac tgatactgaa
     4441 ggatccatca gtgtcaatgg attaatagac ggcaattata ctttagttga aacaaaagca
     4501 ccagatggtt atcaattaac cgatccacaa aaagaaacta cttttatagt aaaaaacgga
     4561 aaattgcaac aaacaaccgg tatcatccat ggcgcagatg aagccaatct tgggaataca
     4621 aacggtgtag tcaatcggga aaatacaact acggataaca cgattactgt agaaaatgtc
     4681 caaaactacg gtgatttcca cttcatcaaa caagatgtga acacgcaaga aaaattagcc
     4741 gatgcagaat tttctattct aagagcaaaa gatccaactg ttaaattaat gaaaaaaata
     4801 gcaaaccttg cctcaggaga atacccatat gcttggtcag atgaagtcac aggttctgac
     4861 tgggaagaag tgaaattgat ttctgattca gaagggaaat tttcaatcga agggctgaaa
     4921 caaggggcat actgccttca agaaacaaaa gcaccagaag gctacgcatt acctaagaat
     4981 gaagctgcgt acactgaatt tacagtagat gatgatccaa caacttctga tgaaacaacg
     5041 atcgataacc aacctaaagg aatcttgcct tcaactggtg gtatggggat catcgtattc
     5101 atcttggtag gtactgcttt agttggagga gcagtgatct acttcaaaaa acgccatcaa
     5161 gaaacagaag cttaaactat tggaaacaaa aatcatacga taaataaacg acagtgattc
     5221 aaatggagag aaaggttgtg acagaagtgg tcggtctctt ggtattcgct caacaaacgg
     5281 caaaatagga ggaactattt ttccgtttgc ttcgctaata ctcaggtcaa ggcgctgatt
     5341 tcacaacctc ttcatataca tattagaaaa taggtggaca tgatgaataa aagcagaata
     5401 agaatactgg acatcttcat ggcaatcatt ttagtcgctg ggatcgggat cttttcttat
     5461 ccttttgtag aagattcact gaatgatttt cttgcccagc aaatgatcat ccattatcaa
     5521 aagcaagcat cgaataagaa ttcagaggaa ataaaaaaac agcaggaaaa aatgacaaag
     5581 aaaaaccaac agttggcaga gcaaaacgta tcaccaggga taggcagttt caaccaagca
     5641 gttgatgcaa aagcattaaa agatttacct agtaatgcct tttttatgga acatatgctt
     5701 ggagtcatcg aaatcccaaa aatcaatgtc agcttaccta tattcgatca gacaacggaa
     5761 atatttttgc aaaaaggaac gtcattattg gaaggaagtt cttatccgac tggcgggaaa
     5821 aacacccatg ccgttctttc tgggcatcgt ggactcccag aagcaaaatt atttacagat
     5881 ttgcctaagt tgaaaaaagg agaccagttt ttcatccaga tcaatggaaa gacattggcg
     5941 tatcaagtag aaaaaatcca agtggtctta cctgatgaag tagattcttt gggtatccaa
     6001 aaaggcagag atctcgtgac tttgcttacc tgcactcctt atatggtcaa tacacatcga
     6061 ctgcttgtga cggggcatcg tattccttat caagcaaaag aggcaaaaaa agcgatccaa
     6121 ggaattgatc aatggaaaaa atggaaattt tttatttggt ttatcggtat actacttgga
     6181 tcaatcggct tagtttggct actcatcgct tatttagatt ctttggccat cgctaagcga
     6241 aactatcctt tatcttttta tgtaaagaac acaaacggac gaccaatcga aggaatggtc
     6301 ttttctgtaa aaacgctcaa cggcaaacac tatattacta gagaaaaagt tccatttgtg
     6361 aaagcttctg atgaatacgg actagtgaga ttttctgatt taaaaggcgg gaactatcgc
     6421 ttgcaacatg aggagctcct gcttaaaatc catgtaaaac ataaacattc gaaacagttt
     6481 tcaatgaaat tgaaaaaagg aagatataaa ctgcgaaaag aaaaagaggt gtactatttg
     6541 attgagaaag aatgaatgaa gatgtccaaa aaagcagagg gaacagacta aatgaaaaaa
     6601 gagagaagct gacgaaatgg acgtgtaaac tgctattttt ggtttactat ctggaacgcc
     6661 agcttctatt tttttatgtt tcagaagaat tttattatta tagtataact tttgaattag
     6721 tttccataac atttggagca aggggttaaa ccacgagctt gagcttcgct taaagtggca
     6781 ggggtatacg taccatttcc acatttatgt gtatggtatt ttgaaccggt cggtgtgaca
     6841 tagacagttt gttcattatt ttgctcagga gctgcggctt gtgcctgctg agaggcctgt
     6901 tcagctgctg cttgctgtcg cgcctgttct gcttgttgct gtgcgattgc ttcttgcctc
     6961 gcctgttctg cttgttgctg tgcgattgct tcttgttgcg cttgttctgc ttgttgtcgt
     7021 gcaatttctt cttgcttttt ccgttcttct tctgcttctt ttgctttgat tgtggaatca
     7081 attgcagcaa gacggcttgt caactcttgt ttgttttcag gtagcgcttg gatagctgct
     7141 gaagcagttg catagttttc ttttgtagga tttgcttcag cttgtgacaa agcagattcc
     7201 gcttctgtca cttttttttc tagtgcagct ttttccgctt tttgtttctc ttcttctttt
     7261 ttcttttcaa tttgttcttg ttctttcgat gaatctgccg tactttcgga gatcgtggta
     7321 ctgctgctgg aagcctgagc tgaacttgtg ctttgagtag tagacgtagt agcaccacac
     7381 ccactcaaca aacttcctgc gaccaaaaac aacgcaacta attttttcac taaatttcct
     7441 ccttaagtaa aaaacaaccg ttttcaataa atgtcctttt caattgttta cttacttgtt
     7501 ataataattg tgttatgaat agtgattgag gaaacttaat ctaatccctt gttgatacac
     7561 gggatttttt gctatagttc tgttattaag tatgcaataa taaaatatgt acgttatttt
     7621 attattagat tattgacatt tatataataa acgattaaat gattcatatc aatagaatac
     7681 ttaggaaaaa aagagaaata ataacaggtt tatgcactat tgacggtgtg acaatatgat
     7741 gtttgtatcc gtttttttct tgtatgtagt atagtaaagt agatacagga ggagaaaata
     7801 tgcagacttt tttaacaaaa gtttctcaaa ataaaattaa aatgattcat ttactgcttg
     7861 aaacagaacg ttggtgttca atagaagaac tacaaaacga attgaaggta tcaagtaagt
     7921 ctatcttgaa ttatctaaca gatttagaag aattgttcca acaatatcct gataaggtca
     7981 ttttgaagaa tgataataat cggcgctttt ctatggaaaa gcaggagaac ttcccaatct
     8041 acattattta tcttcatttc tacagaaaat catataatta tcatttgatt gaattcatgt
     8101 atcaacatcc tgagaaaaat ttggaagatt atgcagacgc tcaatatacg agtgtatcca
     8161 cagtttttcg ttatgctaag ttattagtaa agtactttga aatgacggac gtcaaagaat
     8221 agtcgccgcc agatagaagg aaattagcag ttgaaaaagc tgccctgcat caagtaatac
     8281 gaccagccaa acggaaacag tgtctcatgt ttttgatttg gctggtctta ttaccgcaga
     8341 tgcaagcttt tgccacacct ctttcttgtc atttacagac tcaactatca atgctgcaag
     8401 ctttccctta atgctgtaac accagtaaga gttgtcaaga tcagcagact gactaggtaa
     8461 ataccgctca aaaacaagat tacggcagtt aaactataat gatccatcaa gaaaccgatg
     8521 acgatagaag aaaaaccacc aacagctcgt ccaaggttca taatgaaatt attcgccgtc
     8581 gcgcggattt ctgttggata caaactacta atcactgcgc catatccacc atacatgcca
     8641 ttgataaagt agccgactat cacagcggct gctaccaaac tccactggct atgggctaac
     8701 acaagtagat agacagataa ggatgaacaa attaaaaaca aagaaaaggc tagtttaggc
     8761 cccaatttat ccatgattat gccaaaagtc atcatcccca aagacatgcc taaaatagta
     8821 ctgaccatcc acaatgaaga cccagaaacc gaaagcccta atcgattttg cataattgaa
     8881 ggcagccaat tcattaaccc aaaatagccg gcaatttgga cagttaccat gatactaagg
     8941 ccacatgttt gccaagcaag atgttttgat tcaaacagtc tgctaagttt ggttttcttt
     9001 tcggcagatt gtacagattt gaattccttt ggttcagtca agccgcgacg aatgaacaag
     9061 accatcaaca ccggcagtac acctacgaca tacaacatct tccaaccaaa cgttggaatg
     9121 atcacagcag ctaaaatcgc agccaaagcc gcaccgacct gcgcaccaat accagcaacc
     9181 gaagaagctc gtccaatttg tttttttgaa aaactttcgg aaatcaagga catacacgcc
     9241 ccatattctc ctcccgctcc tacacctgcg ataaagcgaa agagataaac caagtagatg
     9301 tttgaagcaa aatacatcaa taaagaagca atcgaaaaca gaaggaccgt ttgagaaaat
     9361 actttcacac gtccatattt atcagctatg atgccaaaaa aaatacctcc aatgagcatc
     9421 cccaaattag tgatcgttga aatgaaaccc gcttgcgtac cactaatatg aaaagaagta
     9481 atcattgaag ataatgaaaa agctaaaaac ataatatcca tattctctaa agcaattccc
     9541 gccgatgtcg ataataatac ctttcgctga tacgttgtca tttttatacc ccttttccta
     9601 aaatttccct aaattgttaa aagtatatcc aaaaaagaat aaaaaaacaa gactttttta
     9661 tttgaaataa tacacaaaca accgatatag ataatccagc ttagttttaa tagaaatgag
     9721 tattgacttg gagtacactt aaagttgtag aataaataaa atacataagt aaagtggcaa
     9781 gaaattaaga gaaagagtga tcagatgaat atcaaacaag tcagtgaaga aaagggcatt
     9841 tcggccgata cattgcgcta ttatgaacga atcgggttga ttccaccagt taatcggaca
     9901 aaaggcggaa tccgtgatta tacagaagaa gacctaaaat gggtcgactt tacgatctgt
     9961 atgcgaaatg ccggattatc gatcgagtct ttgattgaat atattcgcct atctagcgaa
    10021 ggagaagcaa ctgtatttga gcggcgtaag cttttgattg aagaaagtga acttttgacg
    10081 aaaaaaattg ctgaaatgca agaatgtttg gaacgtttac aaggaaaaat caagaaatat
    10141 gatcgtgtgt tatctagaaa taaaatggaa tgtatcaagt aaaaaaatca aggagcatcg
    10201 ctatggagtt acgcttacta aaatattttt ggacaattgc agaagaagga acaatctctc
    10261 aagccgcaga agttctacat cttacacaac caaccttaag ccgacaactg aaggagttag
    10321 aagaggaact aggaacagaa ctgtttgtac gcgaaaaccg caaaatgatc ttgacagaag
    10381 caggttattt tctaaaaaag ccgagcagaa gaaattttag atttaacgaa tcaaaccact
    10441 caagaattcg agaacagaaa aaaacaatta ttcagcggcc aatttacgat cggctgtgtg
    10501 gaagctgata attcgaatac attggcacta ttactagaaa agttgatccg tgattatccc
    10561 agcgttcaat ttcatatcca cagcggaaca agtgatgata tcacggagtg actagaaaaa
    10621 agcctgttag atttagcgat tttgctagat ccagtagata cagagaaata cgaaacgatt
    10681 ttgttaccaa gaaaggaaaa atggggattg ctcattttag aaaattctgt tttagcaaaa
    10741 aaagacgtga taacaaaaga agactttgcg aatgtacctc ttttgatctc aaagcgtcca
    10801 gaaatacagc gaatgtttgc tgactggtta ggcataaaaa aggagcagtt gctgatcaca
    10861 ggaacgtaca atttgatttt caatgtattt gcgctaattg agaatcaggt aggtgctgct
    10921 ttagtcatag aaggtgctgt aagtcatcat tcaaagcgag tgacattcgt tccgcttgac
    10981 ccaatgattg aaacgaactg catgctagtc tggaaaaaga atcggatttt atcatctgta
    11041 gtcaatgaat tgattcaccg tttcaaagaa gcttttgaac agtaataagt atctctaatc
    11101 gaagaaattt caatacaaaa gcgagacgta tgacagtctt gcttttgtat tttttcattt
    11161 ttaaatcaaa aaaaataaag agatttacat aacttgttaa ataagcgctt acaaacataa
    11221 gaagaacgga aatgaatcag ctataataac cgttgttctg gattgtaatc attacaaaga
    11281 aatgaagatt ataactttgg aggtgttcga aaagaaaaat gggaggagct ttcatgaaaa
    11341 gatttagaaa atgtttgaca ttggtttttg tgttgagctg cttgatacaa ctttctgctt
    11401 tcaagcaagt agtgtttgct gcaagtgaaa aaagtagtgt aggtatcact tttgaaggaa
    11461 gtgttccgca aagctcaacc gatccatccg taccatcctc gcaagatgcc gaacaatcgg
    11521 caaatcaatc cagacaaaag aaatacccaa aaaccaacga gattcagcaa tcatttatta
    11581 cttggggtgg aatatttctt ttagtaatca gtttgttatt aatattttgg aaaagaaaga
    11641 ggaatagaga atgaaaaaaa caattttagc tgcagcatta attagtacaa cacttttcgc
    11701 aggaacatta gctgtacatg cagatggcct aaatgggaaa agtactgcaa agatcggttt
    11761 gacggcacaa gatccagatc atcctgtagg tccaattgat ccaatcgacc ctgatgataa
    11821 tcctccttca aatgatccaa ctggaaatac tggggacttg cgtatcgatt atatgtcaaa
    11881 aatcgatttt gggaaacaaa cgatttcagg aaaaaccgaa acatatactg ctgaaaaacc
    11941 agcgttaaca accgaagtcc aagtttcaga tctacgagga actggtgctg gctggaattt
    12001 acaagttagt tacgatacag aaaaaccagg atttacttca gaagataaaa cattgaatgg
    12061 tgcagaacta acattaccag tgggtacacc agaaaccgtg gaagacaata gttctgcagc
    12121 tcctgctaca agcaaagtag tagtgaataa tgatgcgcaa aacatcatgt cagcagctgc
    12181 taatactggt ttagggacat gggcagatga aatggataca gcaaatgttt cgcttaaagt
    12241 cccatcaggt aacttagtgg gtgattacac agctacactt gtctggactt tgtcagacgc
    12301 accagcttaa ttaaacattt attacgaacg aataaggaga gaataacaac gatgaaaaaa
    12361 caagtattct acacattagc agcgacagta ttgctatcaa gctcattatt aagcacgttg
    12421 aacgtacaag cagatgaaac agagccaacc aaagattctg caacttctac agcaaagatc
    12481 acgtttacag gagataactc tgatccagta aacccggtag atccagatga tcctgacaat
    12541 ccagtgaacc cagtagatcc ggacgaccca gataaccacg gtactggaga taacggtcct
    12601 ttgtcaatcg actatgtctc aaatatcaca tttggcacaa aagatatctc aacgactagc
    12661 caagtataca acgctgaaaa tgcagagcca catgttcaga tcactgataa acgtggagta
    12721 gaaggcggat ggacattaac ggctgcagct acaaaatttg ttgctagtga caattcagag
    12781 ttaaaaggtg cagaattatt atttaaaaac ggagtagcag aaacaacttc tgataataca
    12841 agtaaagcac ctgtaacaag tgatctgatc tttgacaaca ctgaagccaa aacagtcatg
    12901 acagccgata gtttaaaagg tcaaggaaca tggttggatg tctttaaagg aacagaagga
    12961 aacaatacaa atgttcaatt atctgtccct tcaggatctg cgcaagcaaa agaatacaca
    13021 tcaacgatca cttggacact cacagcaggc ccaactgctt agaatggaga gaaaaatgaa
    13081 caaacttcaa tcattatttg ttgctgctct tagtttatta agtattgtag tgatgccatt
    13141 gacgacatac gctgattcaa atgaatcagc gtatagcgtc aatgcaaata ttcccgactt
    13201 tcaaaaaaat aaaaacgttt cttactttga tttgcaatta gatccaaatc aaaaaaaaga
    13261 gatttcaatc catttagcta actcagcaaa agaaaaagcg atttttaacg tagatgttaa
    13321 taatgctgcc acaaacagta acggggtcat tgactacgga caaacagatt tgaagaaaga
    13381 ttcgtctgct aaatatgtat tgaatcagct agttactcct aataatcaag aaattgaatt
    13441 acaagctggt gaatccaaag atgtgacttt ccaagttcaa atgccaaaag atcgattcaa
    13501 aggaatcatt cttggtggaa tacacatcag taaaaaaggt aatgtgagtg aaaaagtaga
    13561 cggaacagca atccgtaata aatatgcgta cgtgatcggt gtgaaactgc aaaacgatac
    13621 agatgccgtc acgcccgatt tgaaactgaa ctcagcaaaa gctggtctgc aaaattccta
    13681 tacgactgtg tttgctaatg tgcaaaatcc aacacctgga atcatcagca aagttaattt
    13741 tgatgcgaaa atcacgaaaa aagggtcaaa agatgttctt tatgaaacaa aaaaggagaa
    13801 tatgtcgatt gcccctaata cgaactttga ctttccaatc agtttgaata aggagaagat
    13861 cgaagctggt gattatacgg tgacgatcga tgccaacgaa ggcaatacag ataaaacatg
    13921 gcatctgaca actgatttta cgatccagtc agaaaaagcc aaaaaaatca acaaagaagc
    13981 agtgacagaa gacaaaggaa tctcatatct cccagctatc atcacagtcg gaatcttgtt
    14041 attattgatc atcgcatttt taagttacaa attacttaaa caaaaaaagt aggtgagtct
    14101 ttcaagtgaa attgaatcag ccactaaata aattatatgt gttaggtttg ctttttatcg
    14161 gcgcattcag cgtgattttt caaagtactg aagtatcggc agaggcaaat tacccagaga
    14221 ttccagaggc aacgattttt gatggatatg tcaaagcatc gatttcttat gacaaggacc
    14281 attcacctaa ctatactgca gttggagata cagtaactgt tacaacaact ttagagaagt
    14341 tagatgatgg tgttttacca gataaatata aacaatcatc agttgttatt ttattttctg
    14401 atgaaacaca aggtcttcaa ttgaaaggag aaccagaatt caaatatgag ctaggtggtt
    14461 tggaagatac ggggaaattc gtcgatccaa cgaaggattt gcaaactaat gtgatctcag
    14521 tttttaattt ttcaacaaaa atcctataca atgatacagc gacatatgat ggaagaatga
    14581 agcaagtccc gatatcgagc acagagctag atgatggtcc atatgacgat acttattcgt
    14641 tatataaatt gttggtggcc aaaggaagta aagtcacttt gtcttataaa gcaacagtta
    14701 cggaagaagc gctgaaaaac aaacaattct cgctccatac agccatatat gatccaacaa
    14761 ttggcggtgg agattttgac tgggaccatt ttctaactac atctatgcca agtatccaaa
    14821 atttagggat ctcatttgac gaagctacta aagacaaaaa aatcgatata aaagacgatt
    14881 cttcttatca aactacttta accggttctt gggctggtgc aaaggacgat atccatcctg
    14941 tattgacgat tgatggtaaa gaagtgccta ttccagatgg tagttttaaa gaagatggta
    15001 catttgaaat cccagttgac ttgaagaata tagggaatat tggaacaaat aatgtccata
    15061 tcaaaatttc tgatagtaat ggacaagtcg cggaagacga tgcggctatt acacttgtcc
    15121 aaacattggt accaccacaa atcaaactct ctgattcttt agcaaatgcc tccatcagtg
    15181 tgtttccaat agatcagtca ttgacgatcg atggggaatg gaaagataaa gacagtaatg
    15241 ctgtttcttt attttataag ctgaatgggc aagaaagagt cttgcaagac aatgtcagca
    15301 atacagcaaa agactcatgg cttcctttct cacaagagat ttctctcgct gatcttaaca
    15361 ctggtgataa tcaagtagaa gtttatgcca aagatgcaga aggccagtta tctaatcatg
    15421 aaagttttaa aatcatattg aatcaaggaa acgtcagctt taaatcgatc aatccggaga
    15481 tccaattcaa taacatggtt atttccggta ccactgttta ttcttcacca caagcaactg
    15541 ttggggtagt tgtagaagat acgacaggaa ttccgaaaga ttggcagctt aaagtcaaac
    15601 agaccacatc tttttctgac ggaacaagag atttgcctgc gcaattgacc tatctgaatg
    15661 gggaagaaaa gctgtcaatc aatgatagct cagcagtgga tttgccagtc gttcaaaaca
    15721 atgctacgga ttattctctg caacaagatg cgaaccatca attccggtta gctgtttcac
    15781 caggcgctta tactggtgaa tatcagtcac agttggaatg gacgatcgag agagcaccat
    15841 aaaaaataag aaggggaaac gtggctgttt ttccttctta tttttgttat gatagaggcg
    15901 aaagggtgga gttagttgat ggagtcaatc atgtttgttc tcattgctta tctaaatttg
    15961 tttatttgga tgagtctgtt gtttaaaggg gcgtcaaaat ggcgattcct cttgttttat
    16021 ttggggctaa tgatagctaa tatgtttttt gttccataca cattcgtttt cttcgcaatc
    16081 gcttatgctg caatgtgttg gtttttatat cttttggtag aaaatatttt tttatcgatc
    16141 ctgttacaaa attttttatt gaatgtggta ggtatttcct ttttcttagc aatcgatatt
    16201 ccaaggtatt tgggatttta tactagctat ttgaatcttt cagtagaatt agtactagca
    16261 actagtttat ttttgctgat cagaaaagca gatcaaaagt atgatatatt cgattatttt
    16321 agtggctata cgaaaaaact gaaaggcttg acgattttat ctgttgtgat ttatgtgctg
    16381 atttacatct ttcatttagg tattagtttt tactcgttgg attatatcct tacttcgatc
    16441 atcacgcttt tatatagtat tttttatact gcttttttta ttgtctttat tatatataag
    16501 aaaaagttcc aagtgatcga actttatttg aaagacaatc aggagacaaa agaatactac
    16561 gagaaattag atgatttccg tcatgattat ttgaattata tttctgcgtt ggaatacagc
    16621 ttgatgaatg acagtcagga gaattctatt aaactattga cccatttaaa ggattattct
    16681 ttagaagcga tcgatgatgc gagacacaat cccttgaagc gaatcagtga tccggcagta
    16741 cgtgggcttt tttatcattt tatggaaaga gccaatcagt ctggcatttc ttatgatatc
    16801 caagttctga atatcatcaa taatatcaag gcagattata tcgatgttct tcgtctgtta
    16861 tctattgcac tgaataatgc cattgagcat tgtgatgaaa caagaaaaga agagccaatc
    16921 agtattacga tcaatcaaac aaaggagcgg ttttatttca aaatcgagaa tcctgctgat
    16981 atgtcctcaa caagcttagc taaattgatg aaaagaggaa gctcaacgaa aaaaaatggg
    17041 ggactcggtc tttataactt ttcgaaaatc acggataaat atctgaatgc caactatgga
    17101 atcaaatatt taaagcgtac gaaaatgttt gtcttggaat tgacgattta tgagaagtca
    17161 atctgtaaaa ctgtatgaat agctaacaaa gtggaataag gaagttaaaa acgggcagga
    17221 cagacatgtc ctacccgttt gtttgtttta gttggtcaat atggcgtaac aattttttta
    17281 cactagaaga acttaaaaga agtgtatctt tatttttgaa atggacttcc atcatttgtt
    17341 tatctacttc tttgatattg aatgggttga tagaaaaact tttcagatca ttaaagaaat
    17401 gatagtcttg gaatttttgt ttgatatttt tcattttatc atgaataacg aagttctcat
    17461 ttgttccata aaattccaag gtggaacgag aaccattgat cgtcgagata tagttgactt
    17521 catctggcgc aatcaaaaag acctttgtgg cgccttgcag ttcaatgata gaggaagatt
    17581 gtttttgagc ttccaatttt tctaaaagta gctgtttcaa tgattgtgca acttcttcta
    17641 agtttgattt atcaagatag cgatctgggc gaatctgctg attaatgatt tctaatccgc
    17701 gtgtattatc gtttgataga aaaataatcg agcattctgg attcttgttc cgtatttttt
    17761 tagcaaaatc aatgccacta tgcaatgtct ttaaatcaat gtcgataata aacaaatcag
    17821 aagaagaaaa gaccagtgta tctaaacgct tataaatcgt ttccaactcg ctgttcgggt
    17881 atatttgaag agagagggca ctcaactctt tatgttttag taattctttc ttcaataatt
    17941 cctgatgaaa caagttatct tcaatgtagt agattttcat ggttcgattc ccctaagaca
    18001 gatttcatta cgattagtat aacagatttt ttctataaat ttcatcatat aaatgatatt
    18061 tgttttttta ttaatcagaa aatatacact tttatcgttt taatggacgg aataagcata
    18121 atgttgatcg cggtattctc ttggcgtaag ggaagttgtt tttttaaaga cctgaataaa
    18181 atggctttga cttgcaaatg aaagcaatac gctgatttct ttataactct tgtccgtaaa
    18241 aagcaacaaa tgtttacttt cattgatctt ccgttcttgg atgtaggagt ggatcgtttg
    18301 gcccatttct tttttgaata aagcagaaag atgtgttttg tgcttgtcaa atgtctcagc
    18361 tagctcgtca agcgttacac ggtcgtaaat atggctgtct atataattga ttagttgctt
    18421 gactagcatg gatttatcgg ctagaggttg ctgatggatc agttgcatga aacgataagt
    18481 cgcttctttc atatatgtgt gccattcact agaagaatgg atacggaaca agccttggat
    18541 caaagcatca gataaactga aggcctgatt aatcgggact ccttcatcga tactaatacg
    18601 cgtcaggatg gcaacgaaag cagagaactt cagcttcaaa ccttctaatt cattttcgga
    18661 caattcgaca tgatacgttt cttgcatctt tttgaagagc caatcaatct tttctggctg
    18721 attccgtttg atatactccg tgtatcgaag ctcgaattga taagaatcaa aagtggcttc
    18781 ttgcatccgg cggtcgtaca aactgtcctc taaccgttga tccgtggtag atcgctgctt
    18841 gatttctttt tggtaaaccc agtgcaattc ctcttttttc aagagttcgt gtgttagaaa
    18901 agcataggta agctggacga attcttcaaa agctgcagtt gtttctttcg tatagtgaat
    18961 accatagtga tagctatgtc ctttgaaaag gaaagtatca ggagattctc cgatcatacc
    19021 gcaagggccg attaataagg aatagggctt gctgtctgta ataaaatcca gtttagcaaa
    19081 cataaaagga aagtgattaa ataaacatac ggattgattc tctttaggcg aaatgaattt
    19141 ttccatgata ggctgtatct cccatgaaat cttcgggtgg caaacttcgc cgcttgaatc
    19201 gaataaaacc atggggatca gatatttttt gtataacaaa tccattattt tagaaatcgc
    19261 tgtctgtttc aattattttc ctcctgtcta tcctttttct tccattgtat cgcaaaaata
    19321 acatgttgaa tattaatttt ttcaggtatc tctcttctat ttttatataa agtcttcttt
    19381 tgttgtataa caaagaaaat tttatcgtta taattaaatg tgtaaaaacg gttacaaaaa
    19441 aaggagaaaa taatggatga aaaacaatta aagcaattag tcgattcctt gacaataaaa
    19501 gaaaaaatcg cacaaactgt tcagttgaac ggcgacttat ttactgatag cgatgtgatg
    19561 aataccggac caacaaagga attaggcttt cctgatgatt ttgatttaag tcagattggt
    19621 tccatttata atataaatga acctgaaaaa ctaaaaaaaa tccaaaaaaa aataatggaa
    19681 gaaaggaata aaaaaattcc gctcctcttt atgtcagatg ttatttatgg tttccgaacc
    19741 atttttccta ttccgttagc acaagctgga tcgtatgatt ttgacttgat tcaaaaagcc
    19801 gcacgagaaa cagcgaaaga gagttattta agcggactgc atgttctatt ttcacctatg
    19861 cttgatttag taagagatcc tcgctgggga cgggtgatgg agtctccggg agaagatgtg
    19921 tatactgcaa aagaatttgc taaacacgct gtccaagggt atcaaggaga caatctccag
    19981 ttgattgaca aataccgagt gggcgcatgt atcaagcatt ttgcaggata cggggctcca
    20041 gaagctggga gggaatacaa tacggtcgat atgtccaacc aacgtctctt caatgaatat
    20101 ctcgctcctt atcaagcggc agttgaagcc aattgtttgc tagtcatgac cgcgttcaat
    20161 gttttgaatg gcgtgccttc tactggtaat cgctggctta atcgggatat tcttcgaaaa
    20221 aggttcggct ttaacggcgt actggtatct gattatgcgg ccattgaaga gttgcaagta
    20281 catggctatg cgaaagatca agcagattca gctaaaaaag cattagaagc cggtgtggac
    20341 ttcgatatga tgacttctgt ctatgccaat tctttagaaa aattagcaga agaaaataaa
    20401 gagatcttgc aattgttaga tgaagcagtg tggcggatct tagacttaaa gaacaaactt
    20461 ggattattcg aagaccctta tcgtgggctc gaagaagaga acacgggaga aatcttgacg
    20521 gaagaatcga aagaaatagc agttaagtta gtagaaaaga gttgtgtctt gctgaagaat
    20581 gaggatacgc ttccattaaa aaaacaccag aaaatcgcag ttatcggtcc ttatggcaaa
    20641 agtcgtctga caattggatt ctgggcttct gtcagtggta aacctcaaga tagtattccg
    20701 ctaaaagatg gaatagaaca gcatttttca aaagaacagc tattatttgc aaaaggattc
    20761 aacctgtttg attcttatga accatttgga ccgttaaaag ctggaattga acaactgaat
    20821 ggaacaatcg aagacgaaga aacattattc caagaagcat tggaaactgc tgaaaaatct
    20881 gacgtgattt tactgacgat cggagaacaa tttttagaaa gtggagaagg agcttctaaa
    20941 actaaccttc gattgcctga aaagcaaaaa cggttgatca aagcgttagc agcattgggc
    21001 aaaccaatcg tggggatttt atataccgga cgtccgcttg tcctaacgga gatcgaacct
    21061 tatttctcta gtttgctgct cgtttggtat cctggaacga tgggaggcat cggtattgcc
    21121 aatcttcttg caggaaaagc agcgccaagt gctcgactca gcatgacttt cccacggagt
    21181 gagggacaga ttccaatcta ttatgcacat gcttcaactg gccgaccttt agaaacaagt
    21241 acgcattcca ctcgctttgt gtccaagtat attgatgaat ccaatgagcc actgtttgct
    21301 tttggcactg gtaaaacgta tggaaaagtc gaagcaagct ggcaggagat acaggatcaa
    21361 gaagataagt tgcaacttgt ttatgaagta atcaatcatt cagcaaaacc aatagaaaca
    21421 gttatccata tttatgtaaa ggacaagaca gcttctattg tgcaaccagt tagacggttg
    21481 attgctagtc aagtcgtttc tatacatgca gatgagaaaa aatcgattaa ctatacatta
    21541 tcaaaagaag cacttatgtt ttataacaat caaggggaaa aaatttggga gtctggcgaa
    21601 tttacttttt ctctctcgac agtagattca gaagatgacc gtacaattca attataaaaa
    21661 aggagaagaa agatggaaaa attagaacag tttttaagta gcagactatt gccgatgtcg
    21721 gagaaattac aaaagaacaa agtgttagcc gcattgatgg aagggtttat ccgaacaagt
    21781 ccaattacat tagggattgc cttcattaca attattggta atttcccatt tcctggctgg
    21841 atcgattttt tgaaaaatgt ggggattttt cctcatgtag aagcaatcac taatggtgct
    21901 acaggtgttt tctcgttgta tgtcgtatat agtttagctt tctcttatgc aaaacaatta
    21961 gggaccaatg aacggaatgc ctcgatcatc tctttggcaa gtttcattat gctgatgccg
    22021 caatccgtca tgacaactgt cacacaagat gggaaagcag ttgaacaagc tgtcggtgcg
    22081 ttacgattgg attatctggg cggacaagga ttatttatcg gtatgcttgt cgctttgctc
    22141 attacccgct tctatgcctt cttagctaag aaaaatatta tgatcaaatt accggaaagc
    22201 gtgccgccaa tggtcacaca atccttgtca ccagtatttg tcgttacgat catttttgtt
    22261 gttgtatttg ctttgcgagt attgtttggc ttgacacaat caggaacgat tttccaattc
    22321 tttatcgacg taatcaatgc cccattgaac tctttagtag cgagtccgtt atcgatcatc
    22381 attatcatgg aattattagc tgttttatgg ttcttcggta tccacaatgc tgttttgcaa
    22441 ggacctttag gcgcaatcag tatgacgatg gtcgttggga acattgctgc ctttcaagaa
    22501 ggaaaagcat taccctattt agttccgtct gttatctaca tggggatgta cgcagctgga
    22561 ttcatgggat tcgtgacatt tttcatgatc aaaagcaaat cagccaaaat gcgccagtta
    22621 ggtaaattat cttttgttcc atccttattc aatgtgacag aaccattgat gtttgggatg
    22681 ccaatcatct tgaatccgat tttctttatt ccacaaatct ttacacaatt gattgcagga
    22741 ttcgttacat ggggattaac cactaccata ttgccgattt ctttgaatcc gactatgtcg
    22801 ctattacctt ggacaactcc tacatttgtg aagatgcctt tatcaggcgg attgaactac
    22861 acgatcctaa tggttatttg tatggcaatc ggtgtggtga tgtggtaccc attcattaag
    22921 atcgcagata agaaagaact agagttagaa gcggcgctgg aaaaagaaga agcagaaacg
    22981 aaagcagaaa aaatcatcaa tgcagaagtc gaagcataat gaaggggaaa agcatgtgga
    23041 cattaagtat cgatatcggt ggaacgtata tcaagtcagc tttaataatg gatacgcaaa
    23101 taagagaaaa aagacaaata gagacaccga aaacggataa ggacaatttt atccttgtcc
    23161 ttgtcgaatt gatctattct tatcagcaga tcgaacccat tgaatttgtc ggattttctg
    23221 ttcctggtgc ggtgaaagaa gcgtcaaccg tcttttttgg tggagcagtc gcatgcctga
    23281 atgaagtcaa tttaaagcaa gaaatagaaa aacatctgcc cgttcgtgtg tttgtagaaa
    23341 atgatgcgaa agctgctgta ttgggagaag caagtttcgg tcatttaaaa gggattgaaa
    23401 atggtgcagg aattattcta ggaacaggcg tcggcgttgg acttttgtta gatgggcagg
    23461 taagaaaagg accccattgt caagctggag aggttagttt cttgatacaa gatcgtggga
    23521 taaatggggc tgaatcattt gcagggatca atttgtcagc tgttcgatta gtaaaagaac
    23581 tggcaaaatt atttcagtgt gaaccagaag gacctcttgt atttgattat ctatatcaaa
    23641 aagaagatga acaagcacaa actttatatc gcacatactg caatcaagta gcgattcttt
    23701 gtttcaatat ccagtgtttg ctggatttgg acaaaataat tattggcggt ggaatcagta
    23761 aacaaaaaag gctgatcaga gatatccaga aaaactatga agccatcttt tctgtgtcgc
    23821 cgatgatcga acaaacgatt acgaaaatga cgatagaagc tgccgccttt gagtctgaag
    23881 caaatctgat cggtgcagcg aaaggagtac gattgaatga gatttccaac tgatttttta
    23941 tggggagctt ccactagtgc gtatcagaca gagggagctt gggatgcaga tggtaaagtg
    24001 ccgtctgttc aagatatgaa agaaatacct gacggaacga gtgatttcaa agtggcggta
    24061 gatcattacc atcgctttga agaagatgtt cgtttattta aagagctagg attaaaagct
    24121 tatcggttct cgatttcttg gagtagagtc atgtctaatg gaaaagttaa tccaaaaggg
    24181 ctggagtttt ataagaaact cattgcttta ttgaaggaaa atgagatcga gccgatcgtg
    24241 acggtgttcc attttgattt gccttatgaa atccatgaaa agggaggatg ggaaaataga
    24301 gcaacgatcc aagctttcgc tgattactgc cagctacttt ttgacacatt cggtcaagaa
    24361 gtaaactatt ggcaaacaat caatgaacaa aatgtcatgg ctttagctgg ttcgatcatt
    24421 ggtacaagta ataaatcaat gaaagaaaag tttcaagaaa accatcatat gctcgttgct
    24481 caagccctag tcacgaaaaa ttatcatgat gggaattatc caggaaagat cggacccgcg
    24541 ccgaacattg ccagtgtata tgctgcttct gataaaccag aagaccagtt ggctgcaatg
    24601 aatatgagtg ctttaagaaa ctggctgttt ttagatgctg cggtttatgg tgtctataac
    24661 cataatgcct ggcacatttt gtcaaaaatc aatgcctgtc ctgaagtaac agaagaagac
    24721 aaaaa
//
LOCUS       DACCKU010000040        24389 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.042, whole genome shotgun sequence.
ACCESSION   DACCKU010000040 DACCKU010000000
VERSION     DACCKU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 24389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.042"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IVN13_001498"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IVN13_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7011994.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IVN13_001499"
     CDS             complement(1551..2972)
                     /locus_tag="IVN13_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7011995.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IVN13_001500"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IVN13_001500"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP7011996.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IVN13_001501"
     CDS             complement(5383..6639)
                     /locus_tag="IVN13_001501"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP7011997.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IVN13_001502"
     CDS             7219..8520
                     /locus_tag="IVN13_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7011998.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IVN13_001503"
     CDS             complement(8589..9188)
                     /locus_tag="IVN13_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7011999.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9442..10323
                     /locus_tag="IVN13_001504"
     CDS             9442..10323
                     /locus_tag="IVN13_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012000.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10336..11946
                     /locus_tag="IVN13_001505"
     CDS             10336..11946
                     /locus_tag="IVN13_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP7012001.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12023..12682)
                     /locus_tag="IVN13_001506"
     CDS             complement(12023..12682)
                     /locus_tag="IVN13_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7012002.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12965..13630)
                     /locus_tag="IVN13_001507"
     CDS             complement(12965..13630)
                     /locus_tag="IVN13_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012003.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IVN13_001508"
     CDS             complement(13777..14382)
                     /gene="dhaL"
                     /locus_tag="IVN13_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP7012004.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IVN13_001509"
     CDS             complement(14410..15393)
                     /gene="dhaK"
                     /locus_tag="IVN13_001509"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP7012005.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IVN13_001510"
     CDS             complement(15395..15790)
                     /gene="dhaM"
                     /locus_tag="IVN13_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP7012006.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15944..16264
                     /locus_tag="IVN13_001511"
     CDS             15944..16264
                     /locus_tag="IVN13_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP7012007.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16376..17245)
                     /locus_tag="IVN13_001512"
     CDS             complement(16376..17245)
                     /locus_tag="IVN13_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7012008.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17341..18645)
                     /locus_tag="IVN13_001513"
     CDS             complement(17341..18645)
                     /locus_tag="IVN13_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP7012009.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18642..19151)
                     /locus_tag="IVN13_001514"
     CDS             complement(18642..19151)
                     /locus_tag="IVN13_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012010.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19326..21460)
                     /locus_tag="IVN13_001515"
                     /pseudo
     CDS             complement(19326..21460)
                     /locus_tag="IVN13_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(21543..23198)
                     /locus_tag="IVN13_001516"
     CDS             complement(21543..23198)
                     /locus_tag="IVN13_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7012011.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23200..23514)
                     /locus_tag="IVN13_001517"
     CDS             complement(23200..23514)
                     /locus_tag="IVN13_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7012012.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23684..23944
                     /locus_tag="IVN13_001518"
     CDS             23684..23944
                     /locus_tag="IVN13_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP7012013.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23958..24272
                     /locus_tag="IVN13_001519"
     CDS             23958..24272
                     /locus_tag="IVN13_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP7012014.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
BASE COUNT         6948 a         5042 c         4401 g         7998 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcata tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaagtt
     9361 ttataaaaag gtataaaacc tttttattaa actatttaat cttgtttagt atatattttt
     9421 agaaaaagga gtggggtttt tatggcagaa atcgtcaaag tcacacattt gaaaaagagc
     9481 tttggtcgtt tcgaagcttt aaaagatgtc tctttcaccg tacattcagg tgaagtcgtt
     9541 ggatttatcg gaccaaatgg ttctggaaaa tccacgacga ttcggacact tttagggatt
     9601 ataaaaaaag acggcggaac ggcaacgatt tttgggcaag atgtatggga agatagttta
     9661 tctatccatg aacgtctttc ttatgttcca ggcgatatct ctttgtgggg aaatttgact
     9721 ggaggagcga tcatcgatct cttcatgaag cttcatggaa aagggagtgc tcaaaaaaaa
     9781 gatgaactga ttcaacgttt ctctcttgat cctaagaaaa aagcaaaaaa ctattcaaaa
     9841 ggaaatcgcc aaaaagtggg cttgattgct gctttggctg ttgattcgga tctctattta
     9901 tttgatgagc ctacttctgg tttagatcct ttgatggaaa cgattttcca agaagaagtt
     9961 gaaaaattga aagaacaggg caaatccatc ctactttctt ctcatattct cagtgaagtc
    10021 gagcgactag cggataaagt cgtgatcata cgcgaaggaa cactcgatga agaaggaaca
    10081 ctcgatgaat tgcgtcattt gactcgttcg accatcacta tagaaacaga agcagagaat
    10141 agtcacttag cacaattgaa cggtgtccat gactttgtgc aaaaaggtca cctcgctcaa
    10201 tttgctgctg ataacgaagc tttcgatgtg attttagctg aagcgtccaa attaggtgtg
    10261 aaaaaatttg aatctattcc accgactctt gaggatttat tcatgagaca ttatgaagga
    10321 tgaggaggaa gcgaaatgac tgataaattt tcacgctggt ccaccctttt ttctcaaaac
    10381 cttagccgtg actggaaaaa gatcctgata tggctagtcg gtgtcactgc tttttctggt
    10441 ggttttgtcc ctgcgtttga agaattgact tccggacaag gattagctgg attatacgaa
    10501 accttgcaaa acccggcaat gattgcaatg gtcggaccaa ctccagcttc gaatgctgct
    10561 gagtatactt taggagcgat gtatgcaaat gaaatgttgc ttttttgcag tttgtttgct
    10621 gctgtgatct ctgttttaca cgtggtgagc caaacgagaa aaaaagaaga acttggtctg
    10681 attgaactta ttcgttcctt tcgagtcgga cgtcaagcaa actctttagc tgtcatcgct
    10741 gaaagtattc tgatcaattt gcttttagca atatcgatca gctgtacgat gatttttttc
    10801 caagcggatt cgatcactgc agaaggtgct tggctttttg ggctatctgt aggtagtatg
    10861 gggattttgg ggagtgccgt tgcactgttt gctgcacaac tgatgccaag ttcagccagt
    10921 tcgaatggtc tgtctttagc gtttttaggt ggactttacc ttctacgagc aataacagat
    10981 gtagctgatt cctccctttc gatgtggaat cctttaggct ggtcttattt gacctatcca
    11041 tttacaacta acaactggat tcctttgctt tatttgtttt tgttcagtct aatcttgata
    11101 atcagttcat ttgttttaga ggaacatcga gatttgaaca ccggctatat ccccgaacgt
    11161 gaaggaaaac cgtatgctaa acgttctctt ttatccttgc gtggtttatt attctatcta
    11221 aataaaggga tgacaatcag ttggcttatc acttttggtt tgatgggggc ggcatacggc
    11281 tctatctacg ggaatatgca acagttttta gaaagtaacg acttaatgaa acagatgttt
    11341 atccaatctg gcgtctcaat tgaaaaaaac tttacaagca cgatcatggt agtcttgatt
    11401 tgtctagctg ctattttacc aattgctagc atcaatcgtt tatttacaga agaaacccac
    11461 ggacgtatga atcagctgtt cgcaacaaaa atttctagga accaacttta ttggaccaat
    11521 atgttactag ctattctatt tgctatattg gcaatcctga ttagttctgg tgcactaggg
    11581 ctttctgctt tggcttcgat gaaggatgcg aaagacatgg atctattgat gtttcttaaa
    11641 gccgggttca atttcctgcc tgccgtttta gtgtttatcg gaattagtgg ctttctattc
    11701 ggatggcttc ctcgaatcgg taaacttgct tatatctact tagcttactc ctttgcactc
    11761 aattactttg gcggaatttt ggatttacca gaatggtttt ccaaaacagc tgtccctagc
    11821 tgggttccac ggatgccaaa cgattctttt gatctttccg tgttccttac gttatcatgt
    11881 atcagccttg cactgttcgt ccttggttct attggttata ggcgaagaga cttactagaa
    11941 ggataataaa aaagcatgta caatttcatg ctagcctcca aatctgggtc aaaaatccaa
    12001 catttggagg tcattttttt atttacaagt cttctaactg ctctttatca atgatccaga
    12061 cttctcgatt ctttcttttt acataaccat tctcctctaa taatttaaat tttctagata
    12121 aagtttcggg ggtcgtaccg ataaatgcag caagttcttt cattttcatt ggcaggaaaa
    12181 agtgatcctt ccctgtcgct ttagctaagt caagcagata gacagcgact cgttcttcta
    12241 ctttttccat catcaaaaaa gttgtctgct gctgtacttt tgttaatttc tcaacgttga
    12301 ttgctaaaag tttcagacta agggcgggat aatgcagtaa gagttccgag aaatcttttt
    12361 gtctcaatat gcaaatttcc gttttttcta gtgcttcgcc aaataaagac tcatttggaa
    12421 cgcctagcaa ctggctctct ccctcatatc cgcccggctc tattactcgt aaaagctgtt
    12481 cctttccgct agcagataat tgatacactt tcatactgcc ggaggccacg atcactaatt
    12541 gaggttctcc attcggcgca agaatctgtt ctcctttttc cacatagtga tgtttgacaa
    12601 gtgcgtgtac tttttcttga tcttgctgta ctaagtgatt gaacaaagga acaaatgaaa
    12661 cacataaatg atcatgactc ataatctctc ctccttctaa acagctattt ctaataaaga
    12721 aacataactt cccttattcc aactgataat tttcttttat catagcataa acgtcaagcg
    12781 aaccagatac cagcaaatag agtggatttc tttttgtccc ttgctttatt ttccttcttt
    12841 tgagacctta agtaattgat tcctatcaat tttttaaata tttacttttt gaataatcaa
    12901 aaacaaacct tcactcgcaa gccgagagta aggtttgttt ttcgcattgc ttatttcgtg
    12961 ttgtctattg aaatttttcc gcacctagct tttgatatag tggtgctagt tcttgtaagg
    13021 tttcagcggt tatttgctca acgtgttcga tttcttgcgg atagaagaat agtttaccca
    13081 tttgaaaggt tcggtctctc atcgcttttt tcaactcttc ttgtttcttt gtatcggaca
    13141 aatagtcaga caaagctaag tggtcgtcaa gttcatgttc tggttgcggc catatgtaat
    13201 aatccttgat atcgactgtt ttgctccact ctttcagtat cgcaagcaac tggttatacg
    13261 ctgcaatgct accatatcgt tcttcgcttt tataatgttg gtacatcaaa tagatttggt
    13321 attgtttttg attcaacaaa actgctagac aggcattctc gtcttgacga gattcgcttc
    13381 ggtacgcgct ccaaaaatga ctgcgtaaat tccagccatt cgtccagctt tcgattttag
    13441 gtttgctcat gccgtacata tcaggcaaaa gtgcagctgt ctggagctga atctctttcc
    13501 atttttgcca gtgttcttta tattctttct tgatttgttc aatcttatct gttggatggc
    13561 tctctttgag ctgtttgaat gcgtagattg gtcgatcaaa caactgataa tctgattttc
    13621 caagttccat tgtttcgcct ctttccgtta tcttttgtat tctacctgaa ttttgcgata
    13681 tggacaaata gaggttgtga aaaagctgtt ctgcatcaag tcataagaac agccaaacaa
    13741 aaaactgcga tgagcatcac cgcagtttta ttgaacttaa ggttgattga tcacatctaa
    13801 aagcgttgaa aataaaatgg cactagaagc agctcctggg tcgatatgtc cgattgcacg
    13861 ctctcctaga taggaagctc gtcctttttt agctaccaag actttggtgt gttcttttgc
    13921 ctggtcaatg atttcttttg ttaaattccc tgccttcaat gcctctgtga ctggcagcca
    13981 aacatctaac atcgttttat cgtcagcttc tgcctttcct cggctttgaa tacccgctgt
    14041 tccctgctcg atcacttcgc ctaatttttc ttgagaatca atagaatcga cctcttttgt
    14101 cgctttcatc atgttcatga atgcagatcc gtataacgga ccagaagccc caccgacttt
    14161 cgatatcatc gccatagaga atactttgaa tgtatcgctg attgtttcag gatgatcggc
    14221 ttgaaaagct tcttcataag ctgaaacccc tcttcccatg ttatttccat ggtctccatc
    14281 gccaatcggg gtatctagat cactgagata ttgtttgttc tgattgattg cctctgcaaa
    14341 ttgcgtgagc cattcttgga tttcttgaac ggttagtttc atattatcac ccttttatct
    14401 tgagattttt taccatgaaa tcgtctgtac attgctttct aaagaatctt tccattgtgt
    14461 ctccgtcaag tcgatcagtg ttagagaaag accttgcata tctagtgagg tcataaaatt
    14521 accgactttt cggaaaacta cattgacgcc tctttcttcc aacagagcta aaacatcatt
    14581 catgaacacg aactgttcca tcaaaggcgt cccgcccatt ccgttgacta gtacacctgc
    14641 ttcttttggt aaattcgagt aatcatctaa aatcttagta acaagttcct ttgccagtac
    14701 tttagaaggc tgcatctttt ctcgacgata gcctggctca ccgtggatac caacaccata
    14761 ctcgatctca tcttctccta gttcgaatcc tggtttccct acttctggta cagttgctgc
    14821 tcgaagcgct agaccgatcg tcttagttgc ctgtacgacc ttttctccca gttctttcaa
    14881 ctcagctagg gaagcaccgt ttcttgctgc atcgcccacg attttgtgta caaggacagt
    14941 acctgcaact ccgcgttttc cagcagtata cgtactattt tcaacggcga tatcatcatc
    15001 tacgatcaca ctttctactt caatatcgtc catagcagct aattcctttg ccatatcgaa
    15061 gttcaatgca tcccctgtat aattcttaac gatcagcaaa actccttttc cactatcggc
    15121 ttccttaata gccgtctgaa tctgatctgg agttggagaa gtaaacacat caccaagcac
    15181 cgctgcactt aacatccctt cccctacaaa cccggcatgg gatggttcat gtccgcttcc
    15241 tccaccagaa accaaaccaa cttgttccgc tttctgattc ttggctacaa cacgggaaga
    15301 ttctacacga tgaacaagtt ctggatagct ttttactaat ccttccagca tttcttcgac
    15361 aatattgctt ggatcattca tgattttttt caatttattc ccatcctttc tttccggcat
    15421 tttgtgcttc tgttaagatt tgctgagtat catctgttat accggcagtg atggctgctg
    15481 caaaagctcc ttccactaaa ggcgcatcca ccagatgata acgttgctgc tgctcttctt
    15541 ccaacatgtc ttttgccagt tccgagttca ataccgcgct accgatatcc gcaaaaacaa
    15601 aataactagc tgcatcgctt gattcattga ctgcttctac gattttcatc gggtctgatc
    15661 cgatctcgcc atttattccg ccgagagaaa aaatttgcac ctcttcggat ttttgcatct
    15721 gctcaatcat ttctttaata ccatctgtta ctttttgact atgagaaata agcaaaatac
    15781 tttttttcac aagaatcttc ctttctttta catagttgct aacgattggt ttcgctttca
    15841 ttaatgaaca attcaagtat ataataaatt tctaattttg ggaaaatatg tgtagtgctt
    15901 cttgaagttt gttatacttt aattaaaaga gaggagtgat cgaatgatta caacaacaac
    15961 gaacagtgta gagggaaaag ccgtagtagc ttataaaggg atcgtttttg gtgaggtcat
    16021 tactgggatc catgcattta aagacttgga ggcggggttg cgaaatatct ttggcggccg
    16081 ctcaaaaagc tatgaaaacg aactaatggg tgcaagagaa gaagcacttg ccgaaatggc
    16141 cgaacgtgca aaagcattag gagctgatgc gatcattggc gtaaaaatgg actatgaagt
    16201 attaggcagc gacaatggca tgctgatggt cacatgtagt ggaacagctg tctccacgag
    16261 ataagtaggg gacatctgtt ttaataacta aagagagatg ggacatgtag tggcactcaa
    16321 gaattagatt tttcagtcta acttcgaggt actctacctt cccatcctct cttttttatt
    16381 tctttttgcg gatgattccg taagctgctg cgactaacac acccgcccct gctaaggaat
    16441 aactgacaga agctgttttt tcatttgttt ttggcaaagt ctgacttgtt gatttggatg
    16501 gctctgtcgt ttgctgaact gtttctgtcg tgcttccaga ggtcactgat ttttctcctg
    16561 ccgaatgcaa aatatctttt attcgttgga aactagctgc taagtcagct ttttttgctt
    16621 caaaatcatt agcaactgtt tgtgcttcgc tgatctttcc gtggattgct tgactggccg
    16681 ctgtgatatc cgcttgagtt tgacttagct ttccagaagc tgtttgagca acctgattta
    16741 attgctgatt gaattctttt gcttgttgga tcttcccgtc aagatctgtt tggacagctt
    16801 ccgcttttgc tttcgcttcg gcaagctttt tctcaagttg tgcacggtca tttcccactt
    16861 tatttttaag atcagctaaa tcttgatcga ttgctgcttg gtcttctttc aatttgtttt
    16921 ttgcagcttc taatgcttct tcgagttctt ttcttttatt ttccaagtct tgcaaatgat
    16981 cgatttggtc agaagaggta gaactattta ttgatgtatc ttctgaagat gaaatcgttt
    17041 ctcctgaatt atctgtcggt acattggaag tcatttctgt ttccgaggca ccgttttcag
    17101 caccagaaga gcttatggtt gttgaaggtt ctgtttgagc aggttcagaa gtttccgcaa
    17161 aagctgtctg tcccaataag aaaatactgg aaacagcaat tggccagatt aacggcttca
    17221 ttcttttttt catcactttt cccatcctta ctacttttta actaatttct tattataagc
    17281 aaaaatgttt ggaagaacca taaacacaac aggctgtttg tctaaaaaac ggtgcatctt
    17341 ttattttttt gtacgttccg ggcttttcca tgtcccgctt ccataaccta aaatcgtttt
    17401 gactgctgga atgaaagtcg tcacgatcgt tatcgcgttc actatccaga acaaaagcat
    17461 atatagtggt gcgaaaagaa ggtatttcaa ttttcttcgt cgatcatcta caatcagcga
    17521 agctagcaac tggaaaaatc ctgcgaccat ttcaaagcag atgaacacaa aactgaatgt
    17581 aaaagtaata taaatctgtt cgtagtttcc ttgatataca taatgcccga ttaatccagc
    17641 aaaaagaact acggaaatac aaaagaatat tgaccagaca atactcaatg tttgatcaat
    17701 gaagatgatc gttcttccaa tatgtttaaa aggatggagc atcacttttt tgaaattcgt
    17761 cagccagact tctgttccac cttttgccca acgttttctg tgtcgatata gcatcttcaa
    17821 cgtcacaggt acttccatga aaaagataat ctcaggagca aaaacagata accagtcatt
    17881 taactgatga tcccaagcaa tactgatgtc ttctgtcgct cgatcttgtc tgaatccgcc
    17941 cacatcgatc aaggcttctt ttcgatacat cgtatttgct ccactgtacg cataaagacc
    18001 accgaaaaca gctgactgtg ttcgtttaat gatgcccacg atacttgaga attcaaccgt
    18061 ttgagatttt gcaatcaatt tggtacgatt ttggacatcc atatttgcag tgaccgcagc
    18121 aatatgtctt gcaccagggc gaatgaaata attcacatac ctaatcagtg catctggctc
    18181 cggtaccgtg tctgcatcat tgcttaaaat caatttccct tttgcaaagg caagtccgat
    18241 attaaacgcg tgggcttttc ctttattttt ttcgatccga accacacgaa gattagcgta
    18301 ttttttcatc agtctagcta atatttctgg tgtttggtcc gttgaaccat cgtctgtcac
    18361 taatacctca tagttgtgat aattcaattt cgtcatcaaa tactcgattg tatattcgat
    18421 cacaatttct tcattatggg ctggcaccat gatcgtgatg aacggttcga cactcaaggg
    18481 aacatctacc cattcttttt gcttatgttt gtataaaaac acataacacc agacaccaat
    18541 aaaccataca aatccgccta aaatcggata agaaacgaga aaaaaataca taatagacaa
    18601 acaagtatct aagaataaat tacctgtatg ggtgattgag atcatttcag ttccacccca
    18661 ttttctttat acaaatctgc aatgaatgtc gtatctagat ttttctcttc ggagacagaa
    18721 taaaaacgga ccgtttctct ctcttctttt gttccaaaac gttccgtata cacttcttca
    18781 agtaattctt gtcttttttt caatttttca tcttcatatt caaaagattt atttactttt
    18841 ttgtatagat ttcgattgtt ccacctagtc aatacaaatg aggtaatcag gaaactgaaa
    18901 aaaatcaata caaaaaagcc gattaagaaa tacagcattt tcttttctga taaaaaagcg
    18961 tacaaggaaa aatccttggc tctttctggt ctcgttaatg ggaaaaagac ccatataaaa
    19021 ggaagaacaa cgatcatcca tcctaaaaga gccactaacg tttgccaaat ctttaaccag
    19081 taattacctt ttgaaaaata cttatctttc accaaaattg atgcaacttc ttctcttgtt
    19141 acttttttca tatcctcacc tcacgttttt ttgttacgac gtaggagttt accatacatt
    19201 tcatttcttt tcacataaag tgttcctaaa tttttcctaa gaaaaagaac aaaacatttc
    19261 tcatcagcaa atgaaaaata ttttgttctt tttatcagtt gttcttttta ttttttatct
    19321 cttaattatg ttgctctttc caatacgaca atgcttttca ttcctctaga tatcgccgtg
    19381 tacatttgtc tgcgtcttct ttgctcatct ccatcgtttt caaatggata taagattaca
    19441 tgatcaaatt ccagtccttt tgctagactc aaaggaatga tttggcagtt ttctaatcca
    19501 tatgaatttt ttttaatcga ttcttgtagc gtttgggttt cctcttctgt ttgggtgata
    19561 actgccgctt tttctccttt tagagaaacc aaaatctcag ctagtatttt caacaattct
    19621 tcagaggatc gaaaagagcg gtattcgggt tcttttcctt gcggtcggat cgatacgatg
    19681 tcgatcttct cttgatccac agcataggtt ttgaaaagct ctgtgattgc tccgctagaa
    19741 cggtaactat ttctaagatc ataccgatgg atcggcagat ggtagtcgtc gaaacaacgc
    19801 ataatatcag caaaagtagt ggaagaatta aagattgcct gattctcgtc acctactaaa
    19861 gtaaagtgag tttttggaaa taattcgatc aatagaccta attgtgccag ggtataatct
    19921 tgtacttcat caatcaatag atagcgaaat gctggtacat ttaccttttc aacaagtaaa
    19981 tgacgaatag ctaatataat gactgcttca tcaacagtac gtgtgggttg ccaagcataa
    20041 gctgagccat atcgtttttc ataaattctt tcaaacaact gatgctcatc cacccatgcc
    20101 atttcttcga tttgtctggt tatttttcga tatttttttg taaaagtttc cttgcataag
    20161 ctacaatact ttgttccgaa tcatcagaaa tcaatttacc gaataattcc aactgcctat
    20221 cttctgtcaa cgagagtacc tgattctgca cagcaggact ttttgcttgt ttcaaaagat
    20281 gattcttcca ttcgctcatc agtgcctttt tcgttgcttg aagcctatcg atcaatttag
    20341 gatgagcagg aactttctca tatatttttt cgatcgtttt tttagaaagg actgtttttc
    20401 catttttagt aagtccacgg ataaaattcg gatgatctaa aaacaaagga tctgattgtt
    20461 ttagattatc gataaacttt ttgctacgca aacgttctgt ctgctccgat acattttctc
    20521 ccgtgatacg tttgaaataa gcctcttcac cttctatttc ctctgctgaa agctgtgaaa
    20581 ccagctgtat gatggttaga ttcagtggat ttctttcacc taatgatggt aggacattcg
    20641 aaatatagtc gataaaccga tgattaggtg atagaatcag caagtcatcg gatgtcatcg
    20701 tatctcgata taaatagagc agatacgcaa tacgctgcat gatagcagag gttttaccac
    20761 ttcctgctac gccatttacc aagatatttt gacttttcac atcccgaatg attgtgtttt
    20821 gttccgattg gatcgtagca gtgatagcct tcatttcatt ggtctcatct tggcctaaga
    20881 cttctaataa tacgtcatct tgaatagcta cagacgtatc aaaaaacttg accaaacgat
    20941 ctctttccaa aagaaattgt cgtctttctt taataaccgt atcgatttta ttttgacgaa
    21001 ccgtataaga agatggacca atcatgttat tgtaaaaaag ttcggcaatt ggtgaacgcc
    21061 aatcataaac aagattttca tctgcatcat ctgtgaaatt tgctgtaccg atataaaagg
    21121 attcttttgg ctcattatcc agaaagtcta caacgacttt tccaaagtag ggacttttca
    21181 ataaacgttc gatctttgct agctgatttt ctgcgttttt ctgtttgata ttcatctgat
    21241 cgatttctct atttttcatt tcaatcatcg aaaaagtatc caggttatct aagtaactgt
    21301 caaaattcaa tcgaagctcg ccagccattt ctttcaacga ctggtttcct tctatttttg
    21361 ccgtatccat cgattcccgc agttcgtttt ttttatcaat tagcttttga tacacgttct
    21421 tcagatatgc ttcttctaac tgtttttgtg tgttgaccat acacattctc ctcttttttt
    21481 aaagtggtgg aaccgattag tttacctctt ttaagagtcg aatgcaagct ttttaacgag
    21541 tatcatttgt taggatatat cagttcttca ggtcggacag tttgatataa agaaaatgca
    21601 ccatcaaggt tttttacgtg gtagccagac tgcttcaaga ttctttccgc tagatagcta
    21661 cgtaatccgc tatgacagct gacgatatat tcttgttggc tgtctaattc atttaatcgg
    21721 tcccgaagtt cattcaaagg gatatgtttg gcttccttga atttgccctt ttccaattct
    21781 tctgctgttc gcacatctaa taaaattttt ccttttgcta gttctgtcgg caattcatgc
    21841 cattgtacgg tttcactaag cccttctgca atgttcattg ctgcatatcc tagcatgttt
    21901 actggatctt tggctgagcc aaatggcggt gcatacgtca attctaattc cggtaaatcg
    21961 aagatcgtca aatgcccttt gattgctgtt gctaaaatat cgatccgttt atccacacct
    22021 tttgccccaa caccttgtgc accataaatc tcgccagtct ctggatggaa gatcaacttc
    22081 agtaaaatat ctgttgcttc tggataatag cttgcatgat cttttcctga tacatgaatg
    22141 acttcaaaag acagttcgtt ttctttggct gttctttcac ttaaaccggt ggatgcggca
    22201 gtcattccaa acgttcgtat aattgctgtt ccaataccgc cttgatttct tcgagaaatt
    22261 ccggaaatcg tgtccgcaac ttgtctacct tgacgattgg ctggtgaagc aagagaaata
    22321 agtgcatctt ttccagtgat ttgctgtttt acaacgatag catcccccac cgcataaata
    22381 tcaggaatat tcgtttgata acgttcgtct actaggatac ccccgcgtat gcctaattcg
    22441 attccagcgg tcactgctag ctggttatca ggacgaacac caatggataa aataactaaa
    22501 tcagcaggga tggtacgccc atcatccagt cgcaatcgtt tcccgtggtc ttcaaatcca
    22561 gcgacagcat gagaagtaat cacttctact ccttgatgag acaattcttt ttctataaaa
    22621 gctgccattt cttcatccag tgttggaaga atatgtggtg cttgttcgat caccatgact
    22681 tctaaacctc ttctttttag gttttctgcc atttccaatc cgatgaatcc tgcaccaacg
    22741 atcacggcac gctttggctg tttttctaat gcatgcatca ctttatcgat atctggtaca
    22801 tttctaatag aaaaaacatt gtcggcttct gttatccctg gaatcgatgg aacaactggt
    22861 tttgcaccag gagataaaac caatgcatcg tactgttcaa tgtgtttttg accctcatgt
    22921 acgacttcta ccgttttgtt ttcgggaaag atggctgtca cttcatgatt tggacgcaca
    22981 tctaaacaaa aacgagcgga taatgattct ggcgtttgga caagaagatt ttcccgttca
    23041 ctgatttctc ccgaaagata ataaggcaag ccgcaatttg caaaagaaac atacggtcct
    23101 ttttcaaaaa caacgatttc tgcatcttcc attaatcggc gaagccgtgt agcagcagac
    23161 attccacctg ctacacctcc gacaataacg attttcatct tatttccctc ctactgttcg
    23221 atcaaaccat tgattcatgc caccacgtac attcactaca tcataaccag atttctttaa
    23281 ttctttagct gcttgtttac tcctcatccc tgattgacaa atcacataga cggtcttttc
    23341 tttgtctcca tcatatctgt ttatactttg taatggaaca tttttagctc cttttatatg
    23401 cccgccccgg tattctgcag gtgttctaac atcaagtaac gctggatttt ccaaaagctt
    23461 ttcctttaat tctgggactg ttatcgattt tattgattga aatagcaaat tcattttctg
    23521 cacctcattt acatttttat aaatacctca tggggtatat taaacaggag ctttattaat
    23581 atgtcaacca gaatgactct tcattttttt cactactttt tatatttgca taaaataccg
    23641 ataaggggta ttatctaatt ataaaaatag aggagaatgt gttatggaga ttgataataa
    23701 aaatatcctg aaccgtttga aacgtacaga gggacaaatt cgtggtatcc aaaaaatgat
    23761 cgaagacgaa aaagaatgta tggatgtcat cactcaatta agcgctgttc gttctagtat
    23821 tgatcgcgta atgggcatga tcgttgcaga taatctgaaa aattgctttg agaaacctga
    23881 tagtaatcca gaagaacaag cggctaaact agaacaagcg atcaagatga ttattaaaaa
    23941 gtaaaggaat gatttcaatg aaagtcgttt tccatattga tgaattagaa aaatggtcag
    24001 aaacagggaa aaacgtaaaa aacctgataa aagcttctcc agaaacgacg attgtcgtat
    24061 ctgtaaatgg catcgcgatt accggttatc tagactctgc caatgcagaa ttcttagatt
    24121 tgcaaggagt cgtttttcac gcttgcgcca atgctatgcg tgctaatcat atttcagaaa
    24181 gttctttacc tgaacaagta atcgttgtac cagcaggtgt ccttgattta gttgaactgc
    24241 aatcccaagg ttatgcttat atcaagcctt agtcagtaga ggttgcgaca gaagtgatca
    24301 gcttcaagaa atttcggctt atttttcgaa gaagctactt ctggaaaacc gtgaatcaaa
    24361 ggttgtgaaa acgctgtcct gcatcaagt
//
LOCUS       DACCKU010000041        23356 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.043, whole genome shotgun sequence.
ACCESSION   DACCKU010000041 DACCKU010000000
VERSION     DACCKU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 23356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.043"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IVN13_001520"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IVN13_001520"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP7012015.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IVN13_001521"
     CDS             complement(1173..1868)
                     /locus_tag="IVN13_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP7012016.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHAELYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IVN13_001522"
     CDS             complement(1881..2597)
                     /locus_tag="IVN13_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP7012017.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTSADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3535
                     /locus_tag="IVN13_001523"
     CDS             2927..3535
                     /locus_tag="IVN13_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP7012018.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3862..4185
                     /locus_tag="IVN13_001524"
     CDS             3862..4185
                     /locus_tag="IVN13_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP7012019.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4367..5737
                     /locus_tag="IVN13_001525"
     CDS             4367..5737
                     /locus_tag="IVN13_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP7012020.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5867..7168)
                     /locus_tag="IVN13_001526"
     CDS             complement(5867..7168)
                     /locus_tag="IVN13_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP7012021.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALVAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7522..9513)
                     /locus_tag="IVN13_001527"
     CDS             complement(7522..9513)
                     /locus_tag="IVN13_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP7012022.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9657..9923)
                     /locus_tag="IVN13_001528"
     CDS             complement(9657..9923)
                     /locus_tag="IVN13_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7012023.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10433..12559)
                     /gene="pnp"
                     /locus_tag="IVN13_001529"
     CDS             complement(10433..12559)
                     /gene="pnp"
                     /locus_tag="IVN13_001529"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP7012024.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12747..13016)
                     /gene="rpsO"
                     /locus_tag="IVN13_001530"
     CDS             complement(12747..13016)
                     /gene="rpsO"
                     /locus_tag="IVN13_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP7012025.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13267..13830
                     /locus_tag="IVN13_001531"
     CDS             13267..13830
                     /locus_tag="IVN13_001531"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7012026.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14073..14594)
                     /locus_tag="IVN13_001532"
     CDS             complement(14073..14594)
                     /locus_tag="IVN13_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP7012027.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14597..15439)
                     /locus_tag="IVN13_001533"
     CDS             complement(14597..15439)
                     /locus_tag="IVN13_001533"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP7012028.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEEPLEESLETLRQKIDTIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15432..16157)
                     /locus_tag="IVN13_001534"
     CDS             complement(15432..16157)
                     /locus_tag="IVN13_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP7012029.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16333..16629
                     /locus_tag="IVN13_001535"
     CDS             16333..16629
                     /locus_tag="IVN13_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012030.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17197..18789
                     /locus_tag="IVN13_001536"
     CDS             17197..18789
                     /locus_tag="IVN13_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7012031.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18802..19542
                     /locus_tag="IVN13_001537"
     CDS             18802..19542
                     /locus_tag="IVN13_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7012032.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19710..21059
                     /locus_tag="IVN13_001538"
     CDS             19710..21059
                     /locus_tag="IVN13_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7012033.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21187..21777)
                     /locus_tag="IVN13_001539"
     CDS             complement(21187..21777)
                     /locus_tag="IVN13_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7012034.1"
                     /translation="MTKSRLEAFTDGVVAIVLTVLVLEIKIPNPSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            21924..23276
                     /gene="brnQ"
                     /locus_tag="IVN13_001540"
     CDS             21924..23276
                     /gene="brnQ"
                     /locus_tag="IVN13_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP7012035.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVDQAPIQPNYQDHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPILMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLHVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         6670 a         4722 c         4457 g         7507 t
ORIGIN      
        1 agggggtgcg acaaaagagc ttcttcaaaa aaataagacg aaatctcaca caaattcgag
       61 aaataatttt cgtaagtttc ttcttattta ttggatctga ccacttctgt tacaacctct
      121 cgttaaacgg tgttcaaaag cttgcatcta cgataataag cccaacccaa cgaaaaacgt
      181 gaggagctgt tttatttgga tgctcttatc gcttgatgca aagacagctt ttttacaacc
      241 tctacttcac ggtgctcaat cagctgtcct tcggtattag ctcaaaaaac ggtaaaatat
      301 aaacagctat tttccgtttc ttctttttaa tacttaggtc aagatgctgt tttcacaacc
      361 tcttgttata aataaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag ttcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ctgatgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagttatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 agaaagcaca ggtgaaggtg gcgaaccaga agtaatcggt tcaacagaag aataatactg
     3541 aaacaatcag gtggtgttag aagcggcggg tcagcagctt ttaacacgcc tttttttctt
     3601 tctaatcgtt tattttcata tgaggcttcc acttttacaa atagctttgc aaacgcaaaa
     3661 atcctaattt accaagcttt tcctcgcata cttttatcgt acctttataa aaatttatac
     3721 tttgaaaaaa agtattcaat tattgttgca tttttcctga ttctttatta gaatgaaccc
     3781 tcgttgaagt aagtatttct agtttatcct aggattaatt ataaaaataa aacattcgtt
     3841 gattcgaagg aggttaaaca gatgtttgaa agttttgata gcagccgcaa ccgttacgct
     3901 tcagttggtg tcgtatcaag tttgccagat gaattgattg acagtatatg gtttattatt
     3961 gacctagatc ttaagggagt cattccatta gaaaatatat taacctttga tttgatcgaa
     4021 aatcgtggaa aagtgacgat gcatttttct caagcaggaa gcaatgtgga aatggatatt
     4081 gatttgccgt ttgcctattc gaaccaattt cctgctcaag tatacgccta tgatgacggt
     4141 acgagagaaa cgatcctttt accaagtgag atcagacaat attaaggtga tagataaacg
     4201 tggatgtccc ttattttaca gaccaggcca agttgttgat tctttgactc aattcttggc
     4261 ttctttttat ggagtttcat aaattatcat tgatttctaa ttggtattct caggataaac
     4321 gctatatact actaactaaa ttttaaagat ataaggagtt taaaagatgc gcgatttaac
     4381 aaaaggaagc cccgctaaac tgattttgat gttcactgtt cctttactga tcggaaatgt
     4441 ttttcagcag ttctacaata tgatcgatat gatcatcgtg ggtcagacgc taggtaaaaa
     4501 tgcgctggca gcggttggtg caactggaag cctgacattt ttgatcattg gctttgccca
     4561 aggtttaaca gctggtttag ccattattac cgcacaacgt tacggtgcaa aagattatcg
     4621 gggtctgaag aaaagttttg ctgctagtgt tgtcattagt cttgtcgtca ctgtgatttt
     4681 aactgtccta agtctcctgt ttattcgtcc gatgcttcaa ttgatgcaaa ctcctcctga
     4741 aatacttgat caagcacaaa catttatttc aattatttta ttagggatct ttgcatcagt
     4801 cagtttcaat ttgctttcta atgtcattcg cgcacttggt gacagccgca caccactatt
     4861 ctttctgatc attgcggtca tcatcaacgt cgtacttgat ttgatcttta ttatctattt
     4921 tggcatgggt gtggaaggtg cagctattgc tacagtaatc gcacaagttt cttccagcgt
     4981 actgtgtcta gtgtatatca agaaaaaaat gcctttgtta cagttacgca aaaaagattt
     5041 caaatttgaa aaagaagagt ttgctgtaca cctaaatgct gctttgccaa tggcgtttca
     5101 atcttcgatc atcgctgttg gtgctatcgt gttacaagca gcactaaata gtctagggac
     5161 agatgtcgta gctgctcaag ccgctgctgg tcgtatcgac caatttgcaa atcagccgat
     5221 gatgtcattt ggtattgcaa tggcaacttt cactgcacaa aactatggtg cgaaagaata
     5281 tggccgaatc ttgcaagggg tacgtcaaac tttactgatg agtgtcggat ttagtctctt
     5341 agcaggtgct atcgtcattt tcttcggtca tttctttgtt caattgtttg ttagcccatc
     5401 agaaacacgt gtatttgaat tagcacaaac ctacttcaat atcaatgggt ctctttactg
     5461 gatcttagca gtcctgttca ttttacgcta cacacttcaa gggcttggac aaagcaagat
     5521 ccctacgatc gccggtatga tggaattgct tatgcgttct tttgcagcta tcgttcttac
     5581 aggaatgtgg ggatacccag gagcagcagc agcttctcct ttagcttgga tcggttctgt
     5641 tgccgtgttg ctttactctt atatgcgttc gatgaaacaa ttgaaacaca tgggtgaaga
     5701 ggaacatttc acattagaac atgcagaagc aagatagcct tgattaaaag aaatcgttgc
     5761 ggctaaaaat gaaaactttt attgaaaata tcttcttcgt ttaccgaaaa caggtttttt
     5821 agataacaaa aaggctgtga caaaaaatgt cacagccttt tttcttttat tctgtttttc
     5881 tttccgtgga ctctactttg aaataatcga tatgtgcatg ctggtattct aataccgtga
     5941 aacggaatga tgcaatctca atgattgttc ctgcttttat ttctttatat cggctagaag
     6001 caaagcccgc aagtgtagta aaattcgttt cttcaaactc tggaatctct acatgaaaat
     6061 aacgctggaa atcatctaat gttagtttgc cggagatgat ataagagcca tctgactgtt
     6121 ccgctaccaa tgcttcgtcc aaatctgtct catctcggat ttcgccgacg atttcttcta
     6181 aaatatcttc aatcgtcact aagccgcttg tccctccgta ttcatctttt aagatagcaa
     6241 tatgctttcc cttttttcgc atgatcgtca ataatttttt tatagggatc gtttcagttg
     6301 ttacgatcgg ctcttcaatc aactgttcta ctgtcagctg gtcatcttta taagattgct
     6361 tgatcaaatc tggcaaggtg acataaccta aaacattgtc ttttgattct aagataaccg
     6421 gatagcgagt atgtccttgt tcaatcgaag cttttatcgc ttctttgacg gtttctgaag
     6481 cttcgaatac ttgcatggac gtacggtcta cttgtatatc ttttgccaag gtatcatcaa
     6541 attcaaacac gttttccaaa taatgatatt cttctttggt caactctcct ttttcataag
     6601 aattcaatgc gactaatgtc agctcttctt gagacaatgt ctcttctcct tcagatacga
     6661 gacgcacacc taatagcttc cctagccctg cagctgaatg attcaatacc caaataaaag
     6721 gaaacattac tttataaaaa taatgaagtg gtttaactac gaacaagacg accttctccg
     6781 tattgacgat actatagctc ttaggaagca gttcgccaat aaccacatcg atatacgtaa
     6841 ctaaaataaa tcccagcgcc acagagattg tactgctaat agccgaacta aaaggtagtt
     6901 taccgatcac tggttctagt aaagccgcga cagatccttc accgacccac ccgatgatca
     6961 aaccggttag cgtaattcct aattgactag ctgataaata gttatctaaa tggtgtgtaa
     7021 catgaagagc tagcttggcg ttctttacat tttccttttc tagttgttcc aatcttgatc
     7081 gtctgatttt cactaaagca aattcacttg ctacaaaaaa tgaagtaatt gcaatcatca
     7141 aaaataaaat aatgaaattg ataaccatat tctcccgcct ttctcttttt ctattgtacc
     7201 ctttttctac aaaaagattg aaaataatgc gtattcatat ttaggaataa aaaaggagat
     7261 gcggcatttt tcagccacat ctccttatca cttcaaaatc attgcgttac ttgttgctaa
     7321 acagcttttt catgacctct gataaaaacg gtattcaaaa gcttgcatct gcggcattaa
     7381 gcccaaacag atgaaaaaca tgagaagctg tttttatctg ctcgttctta ctgcttgttg
     7441 cagtacagct ttttcatgac ctctgataaa cggtgttcaa gtagctgaca ttcgacatta
     7501 gttcaggtca aagcgctact ttcacaaccg ctctttcaac catgttttcc gagtatttcc
     7561 aatgataagc ggaacagttt catgttttac ttcgctgtac tctagcccaa accatgtttc
     7621 tggcgaggta gcgatttttt tgattgccag ttttgcctgc atgatttgac gttgccattt
     7681 tcctagagtt gtcacattgg aaactacttc atcaatcagg acaaattgga aatcgccaac
     7741 ttctcgccct ggtgtcagag agtatctttg tggctgtttt ggcaaacggc cttgtttcat
     7801 caaatcatgg atgacttggc gaacatatac gttgatttcc tgttggaccc tgaaacctaa
     7861 gaacagattc acttggacaa taaaatcagt gtccatcata tctacttgat attctttcgt
     7921 atatggctca tccgtcactt ccacatgaac aaaccaatag actcgtgctc ttttaggccg
     7981 tttatctaaa atcgaataga tgaactgtcg gccaacttta tcttcagcca tatcaggaac
     8041 caagaagacc acatttgtct gatagattgg taaagaagta tcatcacgta attctctaag
     8101 ctgcgggata taatccctca aagacacatc ctcggttgac gattcgcgaa taatgttccc
     8161 ttgttcccaa ataaacatca cgactaagat gatcaaacca atcagaacgg ctacaaaacc
     8221 gcctcggaag aatttgacaa cacttgagat aaagaaaacg acttctatcg caccgaagaa
     8281 aaaggtaact aaatatgcaa gccaacgaga atgcccattt tggataagat aaaagtaaag
     8341 cagtgctgtt gtcatcaaca tcgtcacagt gatcgataga ccgtaagcag cttccatatg
     8401 atgagacgtt cggaatgtga caaccactag agaacaggcg atccataaaa tcagattgac
     8461 tgctgggata tacatttgcc cgatactgtt cccaggatac atgatcttta accgaggcag
     8521 cagtttcaac ttgattgctt ctgaaactag cgtaaacgaa cctgtaatca atgcttgcga
     8581 agcaatgact gcagcgactg ttgcaaaagc aacgccgatc acgatccaac tttgaggcat
     8641 catttggaag aatggattca ggttcttgat ttgctgtgct tcagggtttt gataaacatt
     8701 caacaaccac gcagcctgcc caaaataatt gataaccaaa caaattttga cgtaaggcca
     8761 gctcagacga atatttcgtt tgccgacatg tccaagatcg gaataaagcg cctctgcacc
     8821 tgtcgtagcc aggaaaatat ttccaagaat gaacaagcct aatttgttat ctgcactaaa
     8881 aagcagctgg atagcataat aaggattcag tgcacggata actgtccagt cttgactgaa
     8941 gttcattaat cccattacgc ctaaaaaagt aaaccaagcg aacatgattg gtccgaatgc
     9001 ttttccgaca atatctgtac caaagcgttg gaccatgaat aaaacaaaaa taataactaa
     9061 agtgataatg acgatcacgt tctggttatt accgaaccgg tcaaaaaact gcggaattcc
     9121 ccgcaagcct tcaatcgcag tcgttaccgt cacagcaggg gtcaataccc catctgctag
     9181 taaggcagct cctccgatca ttgcaggaat gatcaaatat ttcccacctt ttcggacaag
     9241 tgtgtagagt gagaaaattc caccttcacc atggttatct gcattcaatg caatcacgac
     9301 atatttgatt gttgtcagta gagtcaatgt ccaaaaaatc aatgagactg atccgataac
     9361 aaaatcagga ttgattcctg acaatccgcc attatcttca acaatcgatt tcataacgta
     9421 aagcggactt gtcccgatgt ctccgtagac gactcccatc gctacaagca ggccagccat
     9481 tgatatcttt ttggtattgt gcgttgttgc cacgtttttc cctcatttct tgttatatgg
     9541 atatccttta ggtaagcttg aatgatagat gatattctta tccatgacac ccgctttcct
     9601 aaattgcttc taattatcca agtaacacac tgaagtgtca acttatttga ataaaattat
     9661 tctttctttg gacgttctct gatacgtctt gccacaaatg aataaagagc aaggcaaaaa
     9721 attgcaccca ataatgctgg tatcaaataa aaaccgccga gttccggtcc cagtggtcct
     9781 agtaaaaatt cgccgagcca actacctaaa aaaccgataa tgatatttcc agatacgcct
     9841 cccgggactt cctttcctaa aattgcgcca gctatcaaac ctaatatacc gccgataaat
     9901 aacgaccata aaaaattcag catctccttt ccctccttta tccatatttc tcacttttat
     9961 tttacgcttt attcagaagg actccaaaaa tatgcgccaa gaaactccag aaaaaaagaa
    10021 gttgtaacaa aagtgttcag cttatttccg tagaagctgc ttctggaaca ccgtgaatca
    10081 gaggttgtga aatcagcgat ttgacctgag tattagagaa ataaacggca aaatagttcc
    10141 tcatattttg ccgttttttg agctaatatc gagggacagc tgattgaaca tcgtttattg
    10201 gagaggttgt aaaaaagctg ttctgcatca agtaataaga acagccaagt aaaaacagct
    10261 cctaatgttt ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt
    10321 attcgcaaaa aaaggactgt gacacggctt ttgtcacagt cccctttttt cgaataatta
    10381 ttgtccccct gtcaccttca cgatcacaag tacattattc ctatgaatta aattattttt
    10441 cttcattttt cgttgaacca tcttcgttca acattgcttt tcttgacaga tttacgcgtc
    10501 cttgtttatc gatttcagtt actttcacaa ggatctcatc acctagttta acaacatctt
    10561 cgactttatt gacacgatcg tttgctaatt gggaaatatg gacaagtcca tcttttcctt
    10621 tgatcaagtt gacgaatgca ccgaattttt cgatacggac aactttccct agataaactt
    10681 caccaacttt gacttctttg gtcaaatctt cgatgatctt gatagctttt ttgatcattt
    10741 ctgcatcagc tgaagcaata cttacttttc catcttgatc gatatcgatt ttcacaccag
    10801 tttcatcgat gattccgttg atcgtatcgc cgcctttacc gataacatct ttgattttag
    10861 caggttcgat ctggatcatt tcgatttttg gtgcatattg gcttaattct tcacgtggag
    10921 cagcaatcgt tgaagtcaat tcttctaaga tttccatacg tgcttgtttt gcttgagtta
    10981 atgcttcagt taggatttgt tctgtgattc cttgaatctt gatatccatc tgtaaagcag
    11041 tgataccatc ttttgtaccg gcaactttaa agtccatatc gccaagatga tcttccagcc
    11101 cttggatatc agttaggatc gtatagtttt caccatcaga aacaagtccc atcgcaatac
    11161 cggcgactgg cgctttgatt ggtacacctg catccatcaa tgctaatgtg ccagcacaga
    11221 tacttgcttg ggaagaagaa ccatttgatt ccaatacttc tgctactaaa cggatcgtat
    11281 aaggaaattc ttcttctgaa gggattactt gtgctaacgc acgttcacct aatgcgccat
    11341 gtccgatttc ccgacgccct ggagagcctg cacgtcctgt agaaccaaca gaaaattgag
    11401 ggaaattgta gtggtggatg aatcgtttgc tgacttccac ccctaaacca tcaatgattt
    11461 ggtgctctcc tagaggtgcg agtgtacaag ctgataaagc ttgtgtttgt cctcgtgtga
    11521 acaaacctga tccatgtaca cgtggcaata agctgacctc tgatgacaat ggacggattt
    11581 catccaattt acgcccgtca ggacggatct tatcgatggt gatcaattca cgtacgacat
    11641 ctttttcaag atcttctgtg atttgtttaa cttctttcaa caagtgatct tcatcttcat
    11701 gacctgcaaa tttctcagca taagcttcgc ggatatcgat cttcacttgc tcgatattat
    11761 cttcgcgagc aagtttttct tcagtcatta ctgctgcttt cattgtagcg taagaagcat
    11821 caaagatttc ttttttcaat acttcatcaa cttgcagtaa agtcacttcc attttttctt
    11881 tccctactgc ttgaacgatt tcttcttgga aagcaactaa ctctttgata gcgtcaaaac
    11941 caaacagcaa tgcaccaagc atgtcttctt ctgaaacttc tttcgcgccg ctttcaacca
    12001 tattgatggc ttgtttcgtt ccggcaacag tcaattcgat atctgtttgt tctgcttgtt
    12061 ctactgttgg attcaagacg tactcgccat ttacacgacc aacttctacg cctgcgattg
    12121 ggccgtcaaa tgggatatct gagattgata gtgctaaaga agaacctagc attgctgcca
    12181 tagctggcga gcagtctgtt tctacactca tcacgatgtt cgttacttgg acttcattac
    12241 ggaagccttc tgcaaacatc gggcgaattg gacggtcgat caaacgggca gtcaatgttg
    12301 cttccgtact tggacggcct tctcgtttga tgaaacctcc agggattttc cctgctgcat
    12361 acattttttc ttcataattg atcgttaatg ggaaaaagtc tgtatctttt gcttctttag
    12421 aggccacagc tgcacttaag actactgtat ctccataacg gactaaaacg gctccgtttg
    12481 cttgttttgc tagctgaccg acttcgattt gtaagggacg gccgccccat gtcgttttga
    12541 atacttgttt ttctgacata acttctcctt ttccttctcg tgaaagcaag acgctactaa
    12601 acaaatgaaa actgccgaaa gccgtctttt gagcagcttt catttggtta gtaggagtcc
    12661 atctagcttg atcacggaat tttacttaat tgatatacga gtaaaaaaag tgagattcat
    12721 gtgaatccca ctctttttga ctaaaattaa cgacgcaagc ctaaacgttt gatcaattcg
    12781 cggtaacgtt gaacgtcagt tttacgtaag taagctaaca agttacggcg gtgaccaact
    12841 tttttcatta ggccgcggta tgaatgatgg tcttttttgt gcacgcgtgc atgttcattc
    12901 aagtggttga tttcttcagt taatacagcg atttgtactt ctggagaacc agtatcccct
    12961 tcatggcgag cgtattcgtt gatgatttca ttcttgcgtt cttttgaaat tgccatttct
    13021 ttcacctctt tcgattattc ccctgcgact gagtaaaacg ttggtgattc gcttaaccaa
    13081 gtggcaggtc atgtgcctag agcacacagc tatactttac ctgaaaccgg gttgaaaatc
    13141 aagtagttaa tcattaagaa aaggtagttt tcaaacaatc ttactactac ttttccattt
    13201 ttgtggtaaa ctataatgcg aaaatagact tgaaacacag tcttttaaga aaggggtaca
    13261 tattccatga ttacaatgga tgatatcatt cgtgaaggaa atccgacctt acgcgaagtt
    13321 gcaaaagaag tttctcttcc attaagcgaa gaagatatct ctttaggaaa agagatgctt
    13381 gagttcttaa aaaacagcca agatccaatc aaagctgaag aactacactt acgaggcggt
    13441 gttggcttag ctgctcctca attagatatt tctaaaagaa tcattgctgt tcacgtacca
    13501 agtcctgacc cagaggcaga tggaccttct ctaagtactg ttatgtataa tcctaaaatt
    13561 ttgagccatt ctgttcaaga tgcttgttta ggggaaggtg aaggctgttt atctgttgat
    13621 cgagaagtac caggatatgt tgttcgccat gccaaaatca ctgtgtctta ttatgatatg
    13681 aatggtgaaa aacacaagat tcgtttgaag aattatgaat cgatcgtcgt tcaacatgaa
    13741 atcgaccata tcaacggtgt gatgttctat gaccacatca acgatcaaaa tccatttgca
    13801 ctaaaagaag gcgtattggt aatcgagtag agaattggca ggttccttcg atactatgct
    13861 acaagaaagc tttataaaaa aggggagtgc tcaaaagctg cacttccctt ttttatgttt
    13921 ctccactaat cgaaatttag atagacatct agaaatcgca attccaaaaa attctgtgta
    13981 ttggatacgc atcttttctt attttttcca tatgggtgtt tatctcagag atgatagaat
    14041 agtttctagg ataaaaagta gcagctacga tgttatcctt cgtagaggat tcctgcttca
    14101 gctagttttt gtttgatgtt tttcagcata tgcttttctg ggtagcggat cgtatgcaga
    14161 tggatgccgc ctgttaattc ggataacaaa gtagcattac ttttttgata acgtctcata
    14221 aaggaatcga tatcttgtgg cgttttgatg tgcaaagttc ctgctaactc accgtaaagg
    14281 ggatgttcca caattacatc aatgatttca ccaccatttt caacgattag tctcagttct
    14341 tcttctgttt gatcttttcc atgctggaca gcgattttac tgatgtgccc agtattggtt
    14401 tcttctgttt cgaggatata acctcttgct gttgccacga tacttttacc tgcagctcgt
    14461 agtaaagcaa tatcgcctac aatgatctga cggctgactc caaaagtttt cgcaaacttg
    14521 ctagcactca ctggtttttc tgtatttgcc aaggtttgca tgatttgttt tcgccgtgtc
    14581 tctccttcga tcacacttat tcctccttca ttttgttttg gtattcgcgc gagtgtttca
    14641 tcaaatcaac atacgtgtct gttattgttt ccttatatcc cttatttttc agcagactaa
    14701 caactttatc caatacttgc tgctctcttt ttgcatcata aactgcttgg ttttcttctt
    14761 ttttcacttg tcctatttta gttaccgtat ccattctttt ttctaataaa gcaaccaact
    14821 ctcgatcgat cgtatcgatc ttttgacgta atgtttcaag agactcttct aacggttctt
    14881 cctctactgt cttgatgata cgagcaggat ttcctgcaat cactacattg tcgggaaaag
    14941 atttggtcac aactgaacct gcgccgacta ctacgttgtc ccctagagtg acacccggcg
    15001 tgacgataac tcctccgcct aaccaaacat tattaccgat tttaatgggt tttgcgtact
    15061 ccagaccgct gttacgtttc actggatgta gtggatgcgt cgccgtgtaa agctggacat
    15121 tcggagcgaa catgcagtta tcaccgattt caatcgtgct gacatctaaa aatgtacaat
    15181 tgaaatttgc ataaaaattc tccccaacaa atatattata tccgtaatca aaattgataa
    15241 ctggctccat atagattttt ttccctgtac ggccaaaagt ctctttcaac aattgggatc
    15301 gcagttccgc tgattctgct tggttgatga tttggctttg acttcttgca aacatacggt
    15361 cggcaaccag ctcaggatca ttggcaaaat aaagctcgcc tgcaatcatt ttttcttttt
    15421 cggttttgaa ttcagtcata atctgctcct aattcttgta tttttttgaa aacaaccgct
    15481 ccgtcttctt gaataaacgt ataaggctcc gctaaataaa caggcgtttg attgattact
    15541 aagattttgg ctgacggctg gcgaaactgt accagatcac aaaacggatg aacttgaaaa
    15601 ctcgttccaa caatgacgat cagctctgct tggctgacgg cataagccgc ctgctccatg
    15661 actccatctt gaaatccttc accatataac acgatgtctg ggcgtatttg tcctccgcat
    15721 gcttgatgtt tgtcgctgtg aagatattct ttccagtcta ccgtttgttg acattttcga
    15781 caatgacatt gatacagact cccatgaaaa ttgacgagtt gcgggctttt agcctttgcg
    15841 tgcaaaccgt cgatgttttg ggaaacaacc caaacatttt tttctttcgc tagatgtgct
    15901 atttccaaat gaatgatatt gggttttgct tcaggatgat aaatcttttt gacaaattga
    15961 tagaatttat ccggttcatt caccattgct tgatgactca gcaagtattc cggctgctcc
    16021 atcccgtggt agattcctga caaagatcga tagtctggca ctccagaagg tgtggatacc
    16081 cctgcaccgg tcaaaaatgt aatttgctga taagctaata tctcttttgc tgcttcgtct
    16141 actgttaatt gtttcatgtt ttgtcacctc actttccatt atagcccata ctcgaacaaa
    16201 tttattgttg aaaagtcaga aaaatgggaa taaaaaagtg ttatgggcat ttgagcaatt
    16261 tatcacaatg ttcactgaca aaagaatgaa aacgtgttac aatactgctg tataaaagtg
    16321 aggtgaatcg atatgttacc attctttatt tattggggcg ttattctggc ttgtatcgcc
    16381 tggttagcgt taagcgttta tttctctgtt ttttatcttg tacgcaaaga aaatggaaat
    16441 ctttgggctt ttgctttatt caacgtgatt gccgcaattg ttttagccat tacattagcc
    16501 gtatatcgaa catggggctg gggaatcacc caatactcaa gtttgattta tctaatatta
    16561 gccatttatg gcgtcgttgt tcttttgcaa gcgatcctag gacgtgagcc aaagaaagcg
    16621 gcagcttaaa tgatcgagat ggaactggac acaggaaatt ttctgttcta gttctttttt
    16681 tattgtatta catacatttt tgtgtataat tatactatcg tttgcaatgg atagatgatt
    16741 cacattttga ttgttttttc ggtaaaagta aagatttcta tatttatcag tattttttca
    16801 aaaaacagca gttatagatt gacaaagcct ctcgagtatc gtaaactttt tttgtataaa
    16861 taactgaata cgagatgcct caatcaatga ggtagaggtc gcgagaatca ttaaccctgt
    16921 ggagtgaagc aaacatggag aagcaggaat agggaggatc gccgaaatgt gcactttttt
    16981 gcttggagtg tatgttggga cgcaagttaa gagcttgcgg actgtcttag cagtgatgct
    17041 aagttgcgct atgttttgtg agaggtttat caccttatgg aattttagcg gcctttttat
    17101 atctataaat cggtcgcttt ttattttttc agataaagct ttaccgcatt tcatctagac
    17161 cgctcgtgta tcatataatc agaaagggag aaaaaaatga aaaaaaatat ggtttcagtt
    17221 gccgtactat ttgcttttgt cttctcacta ttcagtgtgg gatctctggc aaatgcagat
    17281 gaaaaaacac cgagcgatga gtttcgcgta ggtatggagg ctgggtatgc acccttcaac
    17341 tggtctcaac aaacagatga gaataatgcc ttgccaattc aaggccaaaa tagttacgcc
    17401 ggcggttatg atgttcaaat cgctaaaaaa gtagcagatg gtttagggaa aaaattagtc
    17461 atcgtccaaa cgaaatggga tggtctggct cctgcccttc aatcaggcaa gatcgacgcg
    17521 atcatcgccg gaatgagccc tactgctgaa cgacgaaaag aaattgattt taccgatcct
    17581 tattacgaat cacaattagt agtagtagtt caaaaagata gcaaatatgc aaaagcaaaa
    17641 agtttagcag atctttctgg agcaaagatc actgctcagt taaatacatt ccactatact
    17701 gtgatcgatc agataccaga tgtgcagaaa caacaagcaa tggataattt ctcagcaatg
    17761 agaacagctt tagcttctgg aatgatcgat ggttatgtca gcgaacgtcc ggaaggtgta
    17821 acggcaacta gtgtgaacaa agacttggaa atgcttgaat tcagcggtgc agatggcttc
    17881 cagacaaacc cggaagatgt tcagatttct gttggtatgc gtaaaggcga cccggatatc
    17941 caaaaagtaa accagattct ttctggcatc tctaacgatg aacgaaccaa aattatggac
    18001 caagcagtca aagatcagcc tgctgcaact gatagtgaag aaaaacctgg catcatttct
    18061 gatttcacta cgatttggaa acaatacggc aatatgttct tacgaggcgc tggacttacc
    18121 ttgttcatcg cattgatcgg gacagttgtc ggtactacac ttggattact gattggtgtc
    18181 ttccgttcgg tacctgaatc aggaaatgca ctgactcgct tcttccaaaa aatcggaaat
    18241 gcacttcttt ctatctatgt cgaagtattc cgcggaacac cgatgatggt acaagcaatg
    18301 gtcatcttct acggtctggc actcgctttt ggcatttcat tgaaccgaac gattgccgct
    18361 ttgtttattg tctctctcaa tactggagct tacatgacag agatcgttcg tggggggatt
    18421 tttgcagtag atgaagggca gttcgaagct gcgcaagcca ttggtatgac ccatggccaa
    18481 acaatgagaa aagttgttat tccacaagtt ttacggaata tcttgccagc aacaggaaat
    18541 gagtttgtta tcaatatcaa agatacagca gttttaagtg taatcggcgt agctgatttg
    18601 ttcttccaag gaaactctgc ttccggtgcg aatttccaat tcttccaaac atttacgatc
    18661 gtcgggatca tctacttagt catgaccttt acgatcacca gaatcctgcg ggcggtagaa
    18721 aagaaaatcg atggtccttc agcttatacc aagcttgaag aaatcgacaa ttcaaatttg
    18781 caggaataag gaggaagaat catgaatgaa aaaattattg aaatcaaaca tctaagaaaa
    18841 agcttcggtg aaaatgaagt attgaaagat atcagcgtaa cagtcaataa aggtgaagtt
    18901 gtgacgatca tcggttcttc cggttctggt aaatctactt tcttgcgctg tatcaattta
    18961 ctggaaaaac caacaggcgg cgagatcatt tataatggag aaaatgtatt agctcctaaa
    19021 tataatttac ctaaatatcg aacgaatcta ggtatggttt tccaatcctt caacctgttc
    19081 aacaatatga atgtcttgga aaactgtatg tctggtcaaa caactgtcct gaaacgagat
    19141 aaagaaactg ccaaaaaagt agcaatcgaa aatctggaaa aagttggtat ggatcgttat
    19201 atcgaagcca aaccagcaca attatctggt ggacagaaac agcgtgtcgc aattgctcgg
    19261 gcattatcaa tgaatccaga tgtattgcta ttcgacgaac ctacttcggc gttagaccct
    19321 gaaatggttg gcgaagtgct taatacgatc aaagacttag cacatactgg cttgacaatg
    19381 cttatcgtga cgcatgagat ggaatttgca aaagatgtat ctgaccgtgt catttttatg
    19441 gataaaggtg tgattgcaga agaaggtaca ccagaagata tctttgtcca tccgaaagaa
    19501 gaacgtacaa aagaattcct ttcacgtatt ttaaatgctt aggataaata gctgattggg
    19561 acaaagagct gcggtgttta ccgcagttct tttttatgaa ttactcataa aaggtaaatt
    19621 tctgaaaatt ttggaatctt agttgtgttt ttcagaaaac tctcttaata tggaagtaat
    19681 taaacttttt tagttaggat gatttaaaaa tgaatgaaga gcaagaactg cagcgtggac
    19741 tgaaaaatag acatgtccaa ctgatttcta ttggcggagc gattggaacg gggttgtttt
    19801 taggttccgg aaagtctatc cagctagctg gcccttcgat attgctcgcc tatcttatta
    19861 ctggatgcat ctgttttttc attatgcgtg cgttaggcga acttctgtta tcaaacacca
    19921 ataaccattc ttttctggat tttgttgcag aatatcttgg caaaaaagcg gctttcatca
    19981 ctggctggac gtattggttc tgctgggtat cgattgcaat ggccgattta acagccatcg
    20041 gaatgtatgt acgctactgg gcgcctggtg ttcctcagtg gcttcctgaa ctgattgcat
    20101 taggtcttct tctttgcttg aatatggtgg ctgtctcatt gtttggtgag ttagagtttt
    20161 ggtttgcatt gatcaaagtt gtagcgatca ttgcctttat cctcgtagga gcttatatga
    20221 tcctcaccca ctatacgaca gatattggca cagccagtat cacgaatcta tggtctcatg
    20281 gcggattctt tccaactggt gcaaaaggat ttatcttagc tttccaaatg gtaacctttg
    20341 cttttgctgg gatagaatta gttggattag tcgctggaga aacagaaaat cctgaaaaag
    20401 tgttgccgga agctatcaat aatattccta tcaggatcat cttattttac ttaggatctc
    20461 tttttgtcat tatggcaatc tatccatgga acagcttgaa tccagataac agtccatttg
    20521 tagaagtatt ttcggaaatt gggatcacga ttgctgcttc cttgatcaat ttagtcgtat
    20581 taagtgcagc agcttctgcc tgcaacagtg caatatatag tacaggacgt atgcttcgtt
    20641 ctttagcgca agaaggcagt gcccctaaaa aattcaaaaa gttaacaaca catcatgtgc
    20701 cagggaatgc tttgacattt tctactatcg tcattttcat ttctgttatc ttgaattacg
    20761 tgatgcctag tgaagtattc acactagtaa gcagtattgc cactacttgt ttcttgttta
    20821 tctggagtat gcttgtctac acgcacatga aatatcgtaa aagtatcctc ggcaaaaaag
    20881 ctcattcatt taaaatgccg ttgtacccat ttagtaatta tctggtattt ctttacatgg
    20941 cctttgtgtg tgtcgtcttg ttcctaggaa aagatacgag aatcgcttta ttactaacac
    21001 ctgtgtggtt cttgcttcta ttactaatct atcatatgaa atatgaacgt aagaagtaag
    21061 aaagtagttg tgacagaagt ggtcagcttc gagaaataag aaggaattta cgaaaattgc
    21121 ttcttaaatt tttgtgaaat ttcggcttat ttcttggagt taaaagcttc tgtctcagcc
    21181 tcttttctat ttccccggaa acaaccgctg ataataggca tctgcttttt tataggaaaa
    21241 aatccaaata ccagatacaa aaaaacgtcc gaataacccg atcaacggta caaaaaagac
    21301 accggcaatc aagcataagt taatcatcat ggagataaga cttcgacggt cctttgacaa
    21361 aactctcacg acatgggaat ctggtttatt tgctttcatg atttgcctca tcaagatacc
    21421 aaaagaaaaa ctccacatcg tgtatactaa gacataagcc atctcaggcc aaaaagcttc
    21481 agggttacgc ccgacccaac tagtaacaga aggacataaa gtcagccaaa agagcaaaaa
    21541 gatattcgac cagaccactc ggtaattaat tcgttctgcc atctgaaata tacgatgatg
    21601 gttcacccag atcaccgcaa caaatataaa gctgatggta taagctaata atgtatttcg
    21661 gatgacccat aaagaatgaa agctagatgg attagggatc ttgatctcta atacaagaac
    21721 tgtcaaaaca atggctacca ctccatcagt aaatgcttct aatctcgact ttgtcacgat
    21781 gattcctcct cagatgattc agcgctttta ttttatcact tacgttatta aataaccatc
    21841 gttttatata ggaaattttc tgaaaattgt tgcatatttt tcgaaatttt gttactttta
    21901 aaaaaaccct aaaggagtta tgtatgacaa aaaaattatc atttaaagac tatcttttta
    21961 taggctccat gcttttcggt ctattttttg gtgctggaaa tcttatcttt cccgttcatt
    22021 tgggtcaaga agcaggcgct gctacctttt gggcgaatct aggtttcttg gttaccggta
    22081 tcggtcttcc cttcttaggg gtgattgcca ttggtgtatc gaaaagctca ggtgtatatg
    22141 aattagcaaa acggataaat caaacatatg cgatggtttt taccatcttg ctttacttag
    22201 ttatcggacc gttttttgct ttacctcgcc ttgcaaccac atctttcgag attggtttag
    22261 caccatttat cgatcctagc agacagacgc tatatctagc aattttcagt attgtatttt
    22321 ttgtgctggc ttggtggttt tcaagaaaac cgacaaagat attagattat gttggaaagt
    22381 tcctgaatcc tgcatttttg gttttattag gtgctttgct tctccttgct tttttgaatc
    22441 cattaggctc tgtcgatcaa gcgcctattc agccaaatta ccaagaccat gcttttttca
    22501 caggatttac tcaaggatat aacacattag atgcgcttgc tgctttagca tttgggatca
    22561 tcatcgttac gacgatccaa aacatgggtg taacaaaacc tgctgaaatc gctaaagaca
    22621 cgatcaaatc tggtgcgatc agcattatat tgatgggaat catttatact ttgctcgctt
    22681 atttaggtgc tatgagctta ggtagctttg ctctaagtga aaatggcggg atcacgctgg
    22741 ctcagattgc tgatcattat ttaggtactt atggcagtat cttattagca tttatcgtta
    22801 tattagcttg tttgaagacc ggtattggtt tgattacagc ttttgcagaa acattcacag
    22861 atttgtttcc gcaaagaaat tacgcattct ttgttgcact tgctagtatc cttccttgtt
    22921 tagcggcaaa cgtaggcttg acgaatatca ttcaattttc gttgcctatt ttgatgttca
    22981 tctatccatt agcaatgaca ctgattttac tagtgttagt tggtcctttg tttaagcagc
    23041 gtacggctgt ttatcgaatg actacttatt ttacattgat tgcttcgatt tttgatgggt
    23101 tgaacgcttg cccagaaact atcaaacaaa cacctattgt tcaaaatctt ttacatgttg
    23161 cggaaagcta cttgccgttt ttcaaattgg ggatggggtg gatcgtacca gcagttatcg
    23221 gttttgttat cggactgatt tggagtttcg ctaagaaaga agaagtagca gattgataaa
    23281 agaggttgtg aaagagacgt tcagccttga gcaataaaaa gtaattttga aaaatggctt
    23341 ctcacatttt tgcata
//
LOCUS       DACCKU010000042        22920 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.044, whole genome shotgun sequence.
ACCESSION   DACCKU010000042 DACCKU010000000
VERSION     DACCKU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22920
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.044"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(159..443)
                     /locus_tag="IVN13_001541"
     CDS             complement(159..443)
                     /locus_tag="IVN13_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012036.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(461..1351)
                     /locus_tag="IVN13_001542"
     CDS             complement(461..1351)
                     /locus_tag="IVN13_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP7012037.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1371..1589)
                     /locus_tag="IVN13_001543"
     CDS             complement(1371..1589)
                     /locus_tag="IVN13_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012038.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1559..3259)
                     /locus_tag="IVN13_001544"
     CDS             complement(1559..3259)
                     /locus_tag="IVN13_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP7012039.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3526..4299
                     /locus_tag="IVN13_001545"
     CDS             3526..4299
                     /locus_tag="IVN13_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012040.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="IVN13_001546"
     CDS             complement(4380..4949)
                     /gene="thiT"
                     /locus_tag="IVN13_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP7012041.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5015..5117)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5123..6094)
                     /locus_tag="IVN13_001547"
     CDS             complement(5123..6094)
                     /locus_tag="IVN13_001547"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP7012042.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="IVN13_001548"
     CDS             complement(6188..7561)
                     /gene="glmU"
                     /locus_tag="IVN13_001548"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP7012043.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8064..8882)
                     /gene="purR"
                     /locus_tag="IVN13_001549"
     CDS             complement(8064..8882)
                     /gene="purR"
                     /locus_tag="IVN13_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP7012044.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9049..9873)
                     /locus_tag="IVN13_001550"
     CDS             complement(9049..9873)
                     /locus_tag="IVN13_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP7012045.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9846..10526)
                     /locus_tag="IVN13_001551"
     CDS             complement(9846..10526)
                     /locus_tag="IVN13_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP7012046.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10549..11487)
                     /locus_tag="IVN13_001552"
     CDS             complement(10549..11487)
                     /locus_tag="IVN13_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP7012047.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11732..12583)
                     /locus_tag="IVN13_001553"
     CDS             complement(11732..12583)
                     /locus_tag="IVN13_001553"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP7012048.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="IVN13_001554"
     CDS             complement(12737..15310)
                     /gene="mprF"
                     /locus_tag="IVN13_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7012049.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15451..16257
                     /gene="proC"
                     /locus_tag="IVN13_001555"
     CDS             15451..16257
                     /gene="proC"
                     /locus_tag="IVN13_001555"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP7012050.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(16336..17892)
                     /gene="rny"
                     /locus_tag="IVN13_001556"
     CDS             complement(16336..17892)
                     /gene="rny"
                     /locus_tag="IVN13_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP7012051.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(18303..19352)
                     /gene="recA"
                     /locus_tag="IVN13_001557"
     CDS             complement(18303..19352)
                     /gene="recA"
                     /locus_tag="IVN13_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP7012052.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(19461..20702)
                     /locus_tag="IVN13_001558"
     CDS             complement(19461..20702)
                     /locus_tag="IVN13_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP7012053.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="IVN13_001559"
     CDS             complement(20942..21520)
                     /gene="pgsA"
                     /locus_tag="IVN13_001559"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP7012054.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(21729..22613)
                     /locus_tag="IVN13_001560"
     CDS             complement(21729..22613)
                     /locus_tag="IVN13_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7012055.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
BASE COUNT         6520 a         4990 c         3969 g         7441 t
ORIGIN      
        1 ataccgagaa aatggttttt gaacatcact tattcaaaat aaaaaaagat tgtgacacga
       61 attttgtcac aacctttaaa tgtattccta gagcaaagaa gctaagcgaa gaaatccaat
      121 tctgattatt tattgtattt ctttgttcag catccttgct aaaagcccat attggacaaa
      181 gtattcacta cacccatgat ctcatctttt cgagaacgtg ctttttctgt cggatcttct
      241 acctcttctg tgtcaattcg aacaataaat ttccgattca tcgtcacgta agcaccgtcg
      301 ttgatcaaaa taaacagttc gttctcatta tagtctgcac aggcatcttt ccatacgtca
      361 aagccgtttt tttcagaagg gatatcccgt ttaaccacag tttttccact taattcgtgg
      421 aacgttacgt cataatattt cattatctct acctctttcg tcattcaata ttttgtactt
      481 gttctctgat tttttctaag atcgtcttag actggacgac ttgattttta atttcgattg
      541 catttgattt ggaaccgatc gtgttgattt cccgattcat ttcttggatc aggaaatcta
      601 attcccgacc tacaggacat tgtgcttcga taagttcatc tagtttttgt acatggatcg
      661 ccagacgatc cagttcctcg tgaatatctc cgcgttccaa caagatggct agctccgtca
      721 acaaccgttg ctcatcgact ttcgctccaa gaaattcctc cagctttttt tgatacctta
      781 tttgaaattc cttttcatag atttctacaa aactatcgag tttttttagt acttctttga
      841 attcttcagt attctttttc aacactaaag ctaaagcttg accttctttt tctcgactcg
      901 cctcaataga atcaagtgcg cttttcaatg tatctagagc taactgtgcc aattcttcca
      961 tgctctcttc cggtttttct tctaccgtta cgaagttttc gtccagcgcg aagcgttccg
     1021 ccatccgact aattgacact tgattttgac taccgtatcg ttgttgaatt ccgtctgtca
     1081 attgagtgat aagtgaatca atcaattccc agttgatgac gatctgtttt tcgtttttgc
     1141 caatataagc tagattcaca aatacttcga ttcgaccgcg gcttaactgc tgtttaataa
     1201 gctgacgaaa atcgttttca acaaagttca ggattttagg acttcgtatt tgaacatcta
     1261 gaaaacgctg gttgacactt tttatttcaa tatctaactg atacgctttt gtttcgcgga
     1321 tggctttgcc aaaaccagtc atacttttca ttcgacaggg tccttcctca ttacatattt
     1381 tctattgctt tttgcagttg ttcaaactct tcgttcgtca atgtcaggcc tttccccatt
     1441 ttttcatggt ctggcgccca atctcgaaga tcgaattttg gcgggcgacc attccagctg
     1501 actaggttca gttctttacg ccaccccttg ttgttttctg acaatatagc gatttcttct
     1561 acgatttcat atgaaaattc ttgtgccatg gttgttcctc cttgagataa tataaatata
     1621 ggatcagtaa tgtgtcaact ggttgcttaa gcaattcatg ctgcaaacga tccttgcgtt
     1681 ttccttgtag cagataatca aaataaacag ttttcgtgcg ctcattttgt attttttgct
     1741 ggtattttcg ataccttcgc cattcttcag tacttaattg accaatttgt gccaataaat
     1801 catccggcat actttttata tcttgttggc agatcatttg aatcatcatg tctacttgat
     1861 agatttcatt gggcagtaaa aaatgttttt ttgctaaaag ttctagcatc gccaatccgc
     1921 atgtctctgg tacgaccctg ttcttatata aattacgcag tttttgccaa aattttatgc
     1981 ttttgaaaaa agataattct aactgaattt cttttgttaa gtaagacaaa aatcctcgcc
     2041 agtatgttct tgcaggttta acagaatgaa atgtatacca gttcccatag taatagccta
     2101 atcgtttttt catttccaga tagcctaatt gtaaatttaa ttggttcctt tggctatcaa
     2161 aaagcagaaa gctgcctagt gtccacaatg tttgacattt ccaatggata aaaatgtctg
     2221 gcatacgaat ttttttcgct gggcctggcc cttgcacatc tacgacaaat gcctctgttg
     2281 ctcctttgtt aatcgcaata tcaataggta ttttgttgcg atacccaccg tctgcatatt
     2341 tattttttcc gatctttcga taagccatag cgggataaaa tgaagcagag gcgagtatcc
     2401 aatctggtat ttcttctgtt tttgtctgtt ggatatcagt cactacttcc gctagttctg
     2461 gcagtttagt agacaccgta tataaacgta ttttgggatt atgtttgagt aaggacaaat
     2521 ccaaattgtc tttgaccatc ttttccaatg gagcagaaga aaccccgcct tcaattagtg
     2581 ctgttttcgt catttggcgt ctttctcgat agaagcgttc acgaaggtct tcaactgcag
     2641 ccatttctgg taacgcaagc acttgtcttg tcgacagctt tttccataaa ttcacagctt
     2701 ttttcatatc tccttgtaaa atcaatgcgc cgttcaacgc acctaccgag gtacctgtga
     2761 taatctcaaa gttgatatca tgttccttca gcgcctgcca cacaccaatc tgataagcac
     2821 cgtgcgctcc tcctccacct aaaacaagtg ctgtatgata gaccagcttt ttatttaatt
     2881 gtgtggttcc ttgatagccg tttttatgaa aaatacgcaa ccattcttct gatagaggag
     2941 attgaaataa aaaacaacag ttttctttaa taaaaaaacg agcgcaagct tccatatacc
     3001 gaaaaaattt tttccattca aaagtttcac cttcaatcaa cagattatta aaaataacat
     3061 ctcctctact tttagcaata gtaaaataga cggtatattt tttcgatact gctttaaaag
     3121 cacattgctt ttttgcagaa cgccaaaaag cgtctctact ttcttgatga ctttgaaaaa
     3181 atggaagggg ttgttttgct tgctggatag gcgaatacag cgacattttc tgtgaaaagt
     3241 aaactttttt tatctccatc gcgctctcct ttttcttcaa gtataccagt acgtcataaa
     3301 aaggtcatga gtctcgtatt cgactttgta gattccataa gaaaacaatt cataaaacct
     3361 cctttaaata aaaaaataca aaaatcacaa aaattaattg aaaatgagag tgttttcaat
     3421 gaaaacgtga ttttgtaatg gaattttaaa ataaaagaaa caagcttttt taatgcaaat
     3481 gttgtaaaat aaaagagatt aagaggaaat aagaggagga acatgatgac tgagtcagtt
     3541 tatgtacata ttgataccac aagtaatgct gttttaacaa aagggctgac tgctactgac
     3601 tttgcaaaca gtatcgtgca ttttcctcaa aacttgctct tgcttgatcc atctgcttct
     3661 gctggagaat atgaatccca tacagggctg aaagtaatcc gtggtacaga aaatattcag
     3721 cgtttctttt cctcttctcg taacaggcgt ggattcgatg aattgaagtg gatcgacttt
     3781 actgatttaa cgatgctgaa agaattgaca cctttagaaa tttcagaatt attatatttt
     3841 ggtcatatga aaacacatct tcattcgccg tttttttata aactacagaa taattttgta
     3901 tacttcgatc ttaatgatca gctgaatcgg atttattatc gctatttaga tgaattttat
     3961 aggatttttg ctagtaaatt aagttcgatc ttgtcagata agatcaatgt aaaaaaatct
     4021 tttttcaaaa aagaaacacc tgttgaaaaa atcagtgtgg aattattgaa agaaatgcgt
     4081 aatttgatgc aggaagggat cattttttct tttgatcaag ttggtttgat cgatggtaaa
     4141 tatcgtatcc caatctatgt gattgaagat tatatttgga aattaaaagg cacccggtat
     4201 cgagaagagg aaatcatcgc tactttaatc tactctgtca ccgatcgaca ctggcaattg
     4261 gtagaagatg ccgatctttc ttatctgcca ttgaagtaga tctatttctt atcaaaaaat
     4321 aaaaaggaaa caagaaacta ggacatgatt ctgtctcaag tttcttgctt cttttttatt
     4381 tacagctcct gtcgtttatc tggcacaaaa atcgaaggag tcgtttgttg gatccctaat
     4441 aaaacgatga tcgttacaat agctgtactc aacccgctgg caccattcat tacgaaagaa
     4501 aagagccaag gattcatccc ccatagtgca tacgctcccc agaaaaccac tcctgcaata
     4561 aaatgccaaa aatatctgga aagtgcgcca atcaatgcac caaacacgat gctttttcta
     4621 atttgtttcg tattatcgtc tacatagcct ttttggacat ttgaagccat gagccctgct
     4681 gcgcctgcaa aagtataggc aatcgggtac tcaatcaaca cttgaacgac acttaaataa
     4741 taaacttgtc ccaacggaaa atgtaggatg ccccaaatga aacctgccag catccctgcc
     4801 tttgtccctc ttcttaacgc aaaaagagtc aatggaatct gccctaatga aatagaaaaa
     4861 ctgctgccga tctggatcgg aatcaacgac aacaccatcg ctaacgctgc aacaatcgtt
     4921 ccttctaccc aaactctatt ttttcccata aaaaaacttc ctcctttgaa taaaaaagtc
     4981 acaaaggagg aagcagcttt cgaaaatagt caaacagctg tttattcaca attcctacgc
     5041 tcgcataacc gatacaggta cgaagggttt agaaatagat tcaatctcag cccaattgga
     5101 ctcccctttg tgagtttatt tattatttat tttttgtttc aaacaatgga ctaactggtt
     5161 tgttttcatg gatacgtttg attgcatctc ccattagtcc gccaacactt acttcatcga
     5221 ttttgtcgat tttacgatct tctggaagat agatcgagtc tgtaacaact agacgctcga
     5281 tagctgaatc ttctattctt tgtaaagcag ggccggataa tactggatgt gtacatgaag
     5341 catacacact agttgcacca gcttctttca aagcattggc agccaatgtg atcgtacctg
     5401 ctgtatcgat catatcatcg atcagtacac aagttttgcc tttgacttgt ccaatgatat
     5461 tcattacttc tgcaacattt gcttttggac ggcgtttgtc tatgatcgca atcggagatt
     5521 tcaaaaattc cgctaattta cgtgcccgtg tgacaccacc atggtctggt gaaacaacga
     5581 caacatcatc gcctttgatt cctttttcta agaaataatt ggcaataagt ggcgcaccca
     5641 tcaaatgatc gactggaata tcaaagaaac cttggatttg aactgcatga aggtccaaag
     5701 tcagcattct tgttgcacca gctttttgaa tcatgtttgc tactaatttc gctgtgattg
     5761 gttcccgtgc acgtgcttta cggtcttgac gcgcatagcc ataataaggc atgactacgt
     5821 tgattgtttt tgcacttgct cgtttcagtg cgtcaatcat gatcagcaac tccatcaagt
     5881 tatcattgac tgggctgctc gttgattgaa taacatagac atgcgatcca cgaatacttt
     5941 cttcaatgtt tacttgaatt tctccatcac taaattgagt aacagatgat tttcctaact
     6001 cgacgccgac tgctgctgca attttctctg ctaatgggcg attggaattt aacgcaaaaa
     6061 tttttaatct tgggtcaaag taatgttttg acatggggac ctccactttc tttttcacaa
     6121 ctctagaatt ctacctaata aatagtagtc atatttttta aataaatcaa gtgattttgc
     6181 aaaagtctta gtttagataa gggagttttt ttgcatatcc ttctttattg acttgtcttg
     6241 ctctcgcaat tgccagcgca tattctggaa tattgtctgt aattgttgac ccggcagcaa
     6301 tcgaactgtt tttagctacc tcgacaggtc cgatgatatt cgtgctagaa ccaataaagc
     6361 tgtggtcgcc gacagtcgta tgatgtttgt tctttccatc atagttgacg aagacaacac
     6421 cacatccgac attgatttct tctcccagcg tagcatcacc aacataagtc aagtgaccga
     6481 ctttagttcc tttgccgatt tgagcatttt tcacttccac aaagtttccg atatggacgt
     6541 tttcgccaat ttcagctttt ggtcgtaaat gtgcataagg tcccacatct gcatgtttct
     6601 tgacatggct gctttcaatg actgagtttt caatgacgac gtgatcctca atcctactgt
     6661 ctacgatttt tgaatgtgac ccgatgacac aatcagaacc aatgactgta tttccttgga
     6721 tttgtacacc tgcttcgatc aatgtgtctg gaccgatttc tacgcctgca tcgatgtatg
     6781 tcgttgctga atcgacaaag cttacgccat tgaccatatg catctggtta attcgctttc
     6841 tcatgatctc atttgcttta gctaacgcga tccgatcatt gactcccatt gattcttcaa
     6901 aatcatccgt ttgataagca gcaactgtat ggccttcttc ttttaagatt tcgatgatat
     6961 ctgttagata gtattcacct tgagcattgt ttgttccaac tttgcttaac gcatcgaaca
     7021 aagcttcgtt gtcaaaacag taagttcctg tattgatttc ttgtacaaga gcttcttctg
     7081 gtgttgcatc tttttgttcc acgatttttt ctacgatccc aatatggtca cggatgattc
     7141 gtccatatcc tgttgggttt tctgcttgtg ccgtcaagat cgttgcactt gcattttttc
     7201 cttggtgata ttcaaaaaga ttgttcagcg tttcagtagt caataatggt gtatcgccac
     7261 tgataactaa cgttgttcct ttttttcctt ccaagaaaga agcagcttgc aaaacagcat
     7321 gtcctgttcc caattgttct gcttgtaacg catatttact gcgttctcct agttgtgctt
     7381 taacttgttc tgctccgtgt ccaacgatcg taatcacttc atctggtttc gtttcactga
     7441 cccgattaat gatatgttct accataggtt taccagaaac gggatgtaaa actttatata
     7501 attttgactt catgcgggta cctttccctg ccgccaaaat aattgcataa cgtgcgtcca
     7561 actgcgtcac tcctaaatat agtttgctga tttcagttta tcccagtata cccatttttt
     7621 caacaaaaca agtgattttc ttttatccca gcgttttctg gataaaaaaa tagatagacc
     7681 ataacgggcc tatctatttg gtaaccggtg ctctcctgcc aactcctcgg tcacaagtca
     7741 cactactcat aaaacatgaa aagctgtttt ttgaaatcgt ttcttgtgac ttagagttaa
     7801 acggtaggct tacaacctct tcattaaacg tttttcaatc agctgacctt cggtattagc
     7861 tcaataaacg gcaaaatatg aggagcaatt tttccgttta ttgagctaat actcaggaca
     7921 ggacgctgat ttcacaacct ctagataaac ggtgttcaaa agccagtttc tcggtattaa
     7981 gccaaattta tgaaaaaatg tggaaagcca ttttcacaaa atttttctta ctactcgaaa
     8041 ctaagcagct ttttcacaac ctcttattct gaaaaataat ttcctggcac aaccgtaata
     8101 tttttcgttt gagtatctac atctttgaca taaagcagtg acgtataatc atcgatggcg
     8161 cgatgaccgt tgaattttga ttcggcaaat acagtgatgc caacaagttc tgattcgaat
     8221 tcttcaatca agctcttcat tccgttgact gttccgccgc ctttcatgaa atcatccact
     8281 acaaggacac gtgaaccacg tttcaaactt ctcttagaca gttccatctt ttcaattctt
     8341 tcagaagatc cagacacata gttgacactt actgttgatc cttctgttat ttttgagtcc
     8401 cggcgtacaa taacgaatgg cgcattcaga taataggaca cagcttgggc aataggtacg
     8461 ccttttgtgg ctacagtcat tacagcatcg attttttcac caaggtattt agaagcaatg
     8521 attcttccaa cttgacgcag cagttcaggt tctcctaaca aatcagagag ataaacatat
     8581 cctcctggaa gcagacggtc ttgttctgat agacgttctg ctagagattc aatatatttt
     8641 ttcgcctctt catatgaaat ttcagggatg aacaatacac cacctgcagc acccgggatt
     8701 gtttccaagg ttccataccc tcgttcttta aatgttttct tgatgattcc caaatcttca
     8761 ctgatagaag atttagcaga ttcataacgt tcggcaaagc ttgttaagga aatcaattca
     8821 tgcgggtgat tcaataaata ctgtgtcatg tcaactagac gttcactgcg acgtactttc
     8881 acctattcca actcctttta agaaatttta tcgttttaca ttatataagt ttttcttcaa
     8941 aaattcgagc attttatgaa aaaataagaa aatagttcgg tatttattaa aataaaaaga
     9001 agcctataaa aaatttttcg tttttataag gcttcttttc tttataattt agttattttt
     9061 tcttctgaag gcaaccaata ttcgtttgat gatatttaca atcaggaaca aagcaataaa
     9121 gactaatgtg attgttgctc ctggaggtgt atctagataa aacgagctag ttagtcctga
     9181 cagcatccct agaaatccta cacaaacact gatagcaatc acgacattga agcttttgcc
     9241 taatcgcata ccgattgctg cgggaagaac catgattgcg gatatcaaaa gtgcaccggc
     9301 aatcgggatc atcacagcaa tagccacacc agtaatcaca ttgaacatca tcgacatcaa
     9361 atgaacgggt agcccatcca cgtgggcggt atcttcatca aaagtcagaa catacatcgg
     9421 ccgtttgaat aaaaagaaca atataccgat ggcaacgaat aagattccta aaaagatgac
     9481 ttgctcccat gtaatggtca cgatcgaacc aaacagatag gattggatac ttgttgctga
     9541 attccctccg ctcaaattca tcaatactaa cgctagcgcc aaaccaccag ccattaaaat
     9601 ggcaatggag atttctgaat aggttcggta gatcgttcgc agatactcca atatcacagc
     9661 tgctaataca acgactagca tggtcgtaag tgtaggatta aggttcaaaa agaatcctaa
     9721 cgctacccct gctaaagaga catgagaaag cgtatcagcc atcagcgatt gtcttctgat
     9781 cactaaaaac acacctagca tcggggcgat tccggcaatg aagatagcgg caagaaacgc
     9841 tcttctcatg aattcatatg aaagcaacgc cattgagaat cctcctttct caccagtcgg
     9901 atctgtctgt ccgcatactg tttgatatct tcatgatcat gggtgatcat tagaactgat
     9961 ttcccatgga catgcgcact atgtcggaga agacgataaa attcatttct ggattcttca
    10021 tccatacctg ttgttggttc atcaagaaca aataagtcag ggtcagtagc aaaaatacgc
    10081 gctaaactga ttcgctgctt ttgtccgcca gacaattcac ctattcgccg atttcgcata
    10141 tcccacatcc ctacagcttc taaagcacgc tgtacatgtt gatgatcttt ttcagagaga
    10201 cgcttgaacc agcggcctct tggatatctt cctgaacgga ctaattcgat caccgtacta
    10261 ggaaaacctg cattgaaaga tgcaacttgt tgaggaatat aaccgatact taattttttt
    10321 ccttcttgat ttttctcagc aatctttatc attccttttg aaggtttcag taatcctaac
    10381 gatgctttca ccaaggttga cttggctgct ccattttctc cagttaaaat gacaaattca
    10441 cccgcatcta catgataaga gacatcttct agtacaggtt catcatcata gtaaaaggtc
    10501 aaatcagata tttcgatata acgcacctta caacctccat tctaaaactt aattgatact
    10561 ttcttttaaa gctttcagat ttttttccat gacagaaata taattttcgc ctgctttgat
    10621 ttgttcatta gtcaagcttt ctaaaggatt caacacttct agcttcactc ctgtttcatt
    10681 cgacaatgtc tcagctacct tcgaagaggc attttcttca aaataaatca ctttgatgtt
    10741 gtttttcttc acaaattctt ttagctcagc taagcgactt ggtgtgggtt cctgatctgg
    10801 tgaaacgccc gaaatcgcta cttgttttaa gtcgtattga ttcgctaaat aagcaaaagc
    10861 agcatgctga gtgacaaatg tgcggtttgt tgcatctttt agctcatcgg agtatttttg
    10921 atccagtgct tcaagctttt tgatataagc atcggcattc ttcgtaaaag tttccttttt
    10981 atctggatat ttttcgccta attcttcagc aatcgttctg acttctttta tagctaatac
    11041 cggatcgagc caaacatgtg gatcgtattc atgtgaatga ccatgccctt cttctgattc
    11101 ttcatgttcg tctgcttctt gcgattctaa acggtcgatt cctttagctg cttcgatcat
    11161 caaggtctgt ttgctatcca ctgaatcctt taacgaatcc acaaaaaatt ccatgtctga
    11221 actattatac acgaaaacat ctgcatcgga aatttctgct ctttcttttg cacttggttc
    11281 gaaatcatgc ggctccgttc cagcagggat caaaagtttc acgtttcctt catcccctac
    11341 gatctctttt gtaaaatcat acatcggata aaaagtcgta acgatcgtca aatcctcttt
    11401 tttgtctgct tccttgttcg tatttccaca tgctgcaaac agtaaagcac ctatcattac
    11461 cgtagctgtc aaaagatatt ttttcattta tatgctcctt tcaatgaaca aatcggaatt
    11521 ttacgattta ttaacgtgaa ctaatgtatc aaaataaaaa aacacagtca atagaaatcc
    11581 atcaatttct gatttaagca tgtatattct tgattaaatc gtaaaatgga tgagttgctg
    11641 ctcatatcta gccatttcac tacaataaaa aactgaagaa taccatttta ggattcctca
    11701 gtttttcatc acctatttta cttcttatat tttattgcag ggttcgtact aaataaactt
    11761 cctcacaaaa accttttaac ccattatata ctctctgcgc tcttgaataa tgttgacaca
    11821 aggcaaaaac ggttggtccg cttccactca tcaatgcagc atctgctccg tacttgatca
    11881 tacgctcttt gatttgatgg atgactggat gccgctttcc cgttatatct tctaaactgt
    11941 ttcccatttt tttgatcatc tgctgataat cttgctctcg gatggcttca gcaagtccgt
    12001 ggatgtcttg atgttggatg gcttgcgtat ctacttctct aaacactgta cgtgtagaga
    12061 cactcattct tggtttgacc aacacaaccc agcactgtgg catagaaggt aatacttgga
    12121 tttcttcccc tttgccaccg acaaatgccg tacttccgta gacacagtaa ggaacatctg
    12181 taccgacttc caaaccgatt tctgccagtt cttctaacga acagcccaga ttccataatt
    12241 ggttgatccc tcttagagca gctgcacagt ctgtacttcc tccgcctaat ccagctgcta
    12301 ctggaatatt cttcttaaca aagatcttga caccggtttt gatattgtat ttatttttta
    12361 gcaataaagc agcttgataa acattgttcc gttcatctac cggtaaaaat gctttatttg
    12421 tttctacaat gattttgtct tctgttattt cttcaaaaaa tagatgatct gccaagtcta
    12481 cgctcgacat caccatttcc aactcatgat acccatcttg ccgtctatat aatacatcca
    12541 gtcctaaatt tatcttggct ggcgcttttt caatcaattc catcctctca cccattcgaa
    12601 aagtctaagt cgcaagtatt ctatcatagg gaaaaataaa agtatagctt atgtctgcct
    12661 agtggttgat tttgtcatcg tttcaacagc tttcacagaa ttttctcttc ataatccaaa
    12721 caagttctag tgattcttat aattctgtga gcacttcttc tgccaaactc ggaacatttc
    12781 ctttttcttt tcctatcaga ctgagtagct gcatggtagc aaatccgaaa ttacttcctt
    12841 tcctataagc aaaataacgt ccttcccaat gatcgacata ttttaatttc ttttcataag
    12901 cagactggaa ggaatagaaa aaattaccat aattatatac gatatgcatg actttttctt
    12961 gaaagaaaga aaaatctgtt tctccaacat tagctaatgg agctaagcct agattggctt
    13021 ccgagtagcc cattttctcg tatacaataa acagattggc taacagatag tcagctaatt
    13081 cctcagattc tttaggtaag acccgcaaca aatcatatcc cgcctgcttc ttcgtcatcg
    13141 gctgagcggt gatgaagcca acgacttttt gatctttacg gacaataccg acaccgctag
    13201 ataatacata tgatttatca aatcgtccca cagagaagta tttttctcgt gcaccttgaa
    13261 gccatttttg tgaaacttca tttacagctt gatgtaattc ttccggcagt tgatcgtacc
    13321 aagtaaaatg gtatccttct ttcatcaaac gttgatattc aagttgctga ctcagccgag
    13381 acaagtcttc tgttgctaga tcaacaatcc ctgcttcacc tactttcgtg aagtgaaaac
    13441 ccaattcatg aagcatcact acgtagcttt ctgatatctt ataaaacgca agctgatagc
    13501 ctaaacaatc tgcttgtttc ataaactgat tcgttgctgc ccgcatcatt ttaggattgc
    13561 caactggatc tcctaaaaca aagcatttgt ttcctttgat ctgataacca aaaacgactt
    13621 gatcttggtc gtcttcttgg taataaaaat aactgtaacc tgacaagcga agctgatggc
    13681 ttgtagctgt gcctccgtat gtttccaata aacggtcaaa acggtctttt tcgtactcac
    13741 tgccaagctt cgtagtatct tcagctaaat attgataaag cgccactaaa gctagcaatg
    13801 agatgcccaa accaataagc cctgagaacc acacatcatc tgaaggaaac aaaataaaat
    13861 gatttcctgt tattcgaccg actaaaaagc gtccgctgtg atagcctgca attgcgtaaa
    13921 cgataaacaa aaagccgaac aagctagcat caaagattat agctccccaa gaataaacga
    13981 attttgttcg ataaaattct tttctggata accacacagc taacagaatc aagacatata
    14041 ccaatatcaa ctgccaagat aacgtacgtg tgattgtatt taaaatacca aaagatagta
    14101 aagcaatcgt aggcagaaat gcctttttga cttttactga aagtgctcta gcaaggccga
    14161 gtaataaaaa gccaaccaat agattcactg tctgatctaa aaaattgaat gagaacggca
    14221 ataatacttg aaatagccga ctgattgcag acagattagt gattgttgat agaagtacca
    14281 tcatgatccc cgcaaaatag acagcactta cgagcacgat atgcgcaacc ttttggaaaa
    14341 acagtcgcgg aatgttatct aaaaatcgat tgattttgac tcctgtatgt gtgatgaaca
    14401 atagaagtcc cgtcataaaa ggtagaacat aataaaacaa ccgataatat aatagccaaa
    14461 ctactgctac atcttgccga acaggcaatt gactcattcc taagatcatc aaaacgtcaa
    14521 aagtccccat tccacctggg accatcgtca gcattcctat caatgtagca atcatgaaca
    14581 taggatagat cgaaagcatc gaaatatcta ttctcatcaa cttgccgatg atcaaaaata
    14641 ccgcaagggc tcccgtccat tgtccaaagg atgccagtag cagaccaagc aagccttttg
    14701 gataaaactc gaagaacaat gtctttctct ttagataagc aaaaaagaaa atgactggtg
    14761 ctaaaaaact accgccgaaa agccagatcc aatagcttcg aaaaatactt tctgactgaa
    14821 taaaaaagac atcaataaat gtaagaaacg accatacaga caaaccagtc agcataaata
    14881 gtgcgacttt tgatacagtg gctaaaacca tttttctatt tgtctgcttt ccataaaaac
    14941 tagcccgcag acttgctcct accacaccac caaaaccggc gagattgttg atcgtgtttg
    15001 ttacccacgc agcaagaaga aactcttttc tttccatttt tggttttcct tgtttttcta
    15061 agactctgat cgtcacccaa tcatacaaca acatcggagt gacacccagc agtccagcca
    15121 gaatcatgag caggatcgtc atgcggcttt gttgtcccat cctttgccaa atatcttgcc
    15181 aactcatgcc attagcaata tttgcgactt gatttgccac aaagactaac acggagccga
    15241 aaaaaatcag ttttaaaatc agactatgtt cctttatcca ttgcagcatc aagtatcgtt
    15301 tatttttcac attttcccat tcctaacttg tatacttttg tgtcataccc caatgttatc
    15361 ttatttattt taatttttcc actttagacc tgttctttgc ttgcgatgat agaaaaagaa
    15421 agatatccta atgctaaagg agaggataac atgaaagtag gatttatcgg cacagggaat
    15481 atggcacaag ctattattga tggtttatta aaaaaaggaa cagtctcgaa agaagaggtg
    15541 ttcgtttcca gtggacattt tgaaaatgca caaaaatttg ctgctgaaac tggcgtccat
    15601 gcatgcaaaa gtaatcagga agttgcatca caatcagata tcgttctttt agccgtgaaa
    15661 ccaaaaatca ttgaaaaagt attggaagaa ctaagagaat atagccagaa agtcttattc
    15721 atttctattg cttcaggaaa atcattaaca gatttagaaa cagcgcttga agatcctcag
    15781 gcctccatcg tacgtgtgat gccgaatgtc aatgtcagcg taggagcagg cgtttctgcg
    15841 atttgcaaaa atgaagctgc cacggaggaa cagcttcagc aagctacgat gatttttgaa
    15901 gcaatcggca gcgtctatcc tttagcagaa tcagatttca gtacattcat tggccttgct
    15961 ggcagctcac ccgcattcgt ttacatgctg atcgatgcga tgggacggac tggtgtactg
    16021 aatggtttgt ccaaacagac agccactgag attgctgcaa aagcaattat gggttcttgt
    16081 caaaaattcc tttcaagtga taaaaatcca tgggaacttg tagaccaagt ttcttctcct
    16141 ggcggtacaa ctgttgcagg tgtagttgcc ttagaagaag caggattcat cccagcaatc
    16201 atcaaaggaa tcaatacgac gatcgagaaa gatcttgaga tgttgaataa aaaataatga
    16261 tagcaatgtt ttttcgtgcc ttacaaaaaa agaagagctg tggcttttgc cgcggctctt
    16321 cttttgtatt ttttattatt tagcataatc agttgcacga gtttcccgaa taacgattac
    16381 tttgatgtgt ccaggataat ccagttcttc ttcgattcgt ttacgaatat cacgtactaa
    16441 acgaacagcg tctaaatccg agatttcttc tggcttgacc attacccgaa cttcgcgtcc
    16501 agcttgaact gcgtaacttg attctacacc ggcgaagctg ttcgaaatat tttccagact
    16561 tcttaaacga cggatgtagt tttctaatga ttcacttcgc gcaccaggtc ttgctgctga
    16621 gagcgcatct gctgcagcta caaggacaga gataacagaa gtagcttcca catctccatg
    16681 atgagaagcg atcgcgttga ttactactgg gttttctttg tacttggcag ctaattccgc
    16741 accgatctca acgtgagatc cttcaatctc atggtcaagt gctttcccaa tatcatggag
    16801 caacccggca cgttttgcta attggatatc ttcgccaagc tctgctgcta agataccagc
    16861 taattttgca acctcgaccg aatgcttcaa gacgttttgt ccataactag ttctgaatcg
    16921 cagacgtccg atgatcttga ttagatctgg atgaagtgta tgagcaccga cttcaaacgc
    16981 tgcctcttcc ccgtattcgc gaatacgttc atccatttct ttacgagatt tctcgaccat
    17041 ttcttcgata cgcgctggat ggattcgtcc atcttgtatc agtttttcca aggtcatccg
    17101 tgcgatttct cttcggattg gatcgaatcc ggaaagaaca actgcttccg gtgtatcatc
    17161 gatgattaaa tcgataccag tcagcgtctc aagcgttcga atattacgac cttctcgtcc
    17221 aatgatacga cctttcattt catcattcgg aagggtaacg actgaaacag tcgtttctga
    17281 aacttgatct gctgcgcagc gttgaatcgc taatgataat aagtttttcg ctttgcgatc
    17341 tgcttcttct ttggcacgct gttcagattc ttttaccatt agagttaact catgattcag
    17401 ctcttcttca gtcgatttca tgatgacatc tcgtgcgtcc tcttttgata aaccagcaat
    17461 tttttctagt tctttttgtt gatcttcaat cattttttca acgtcttttt ctcgctcttc
    17521 aattaattgc tgttttgaac taagtgcttc ttctttactt tcaagtgagt tctcgcgttt
    17581 ttctaacgag tcatctttac gattcagcgt ttgttcgcgt tgaatcaaac gattctcttg
    17641 agattttaat tctaacttac tttctctcag ctcactttca acttcagaac ggtatttctg
    17701 attttcttcc ttagcttcta acaatgcttc tttttttaga gtttctgctt ctttttttgc
    17761 actatcgatg attccagcag ctgatgattg tgcaccagct atttctttct cgtgacgtga
    17821 ctttgcgacc ataaacccta aaccaagacc gacaattaaa ccgatgatag cgaggagaat
    17881 gttgaatacc ataaaatcca cctccatact atctttttca tttttatcat ctatacagct
    17941 tctatgataa attattctaa actcgtcaat atgcatcttc gcatatatgt gtacaaaaaa
    18001 catgcacaac tttattctaa tgtttgtacc ccgtagtgtc aactttaaat aaacaaagaa
    18061 acggctttct acttattcat taaacgatgg tttttaaaat aaatgacact agtttcatat
    18121 aggtaaacct ttaaattgag aaacttttct attaaattat tctttgtaga tgaacagata
    18181 tttttatttt atgtacccac tcgtataatt aaataactca cgatttcttt attcaataaa
    18241 aaaacggcag gtcaaggaga gtacctactc ttcagaccag ccgtttttgt ttatcataat
    18301 ttttattctt ctactaaagg caattcttct tgtccttttt catcttctgg gacagcgact
    18361 tcttcaccaa taccataagc tgctctaact agagcagaaa cttctgccat catttcagga
    18421 tgattagcca tatagatttt agcgttttcc cgtccttgac caatccgatc ttctttataa
    18481 gaataccaag caccgctttt atcaacaatg tctttttcta ctgccatgtc tagaagttct
    18541 ccttcttgtg agatccctaa accatacatg acatctactt cagcgatttt gaaaggaggt
    18601 gctactttgt tttttacgac tttgatcttt gtacggttac caacaatatc tgtaccttgt
    18661 ttcaattgtt ctgcacgacg tacttccaaa cggatcgttg cgtagaattt caatgcgcgt
    18721 cctccaggag tgatttctgg attcccaaac atcacgccta ctttttcacg gatttggttg
    18781 atgaaaatag cgattgtttt tgttttgttg atcgaaccag agagtttacg caatgcttga
    18841 gacatcaaac gtgcttgtaa cccgacatgt gagtcaccca tttcgccgtc gatttctgct
    18901 cgtggaacta aagcagcaac tgaatcgact actacgatat ctacagcccc acttgatact
    18961 aaagcatcag cgatctctag accttgttct cctgtgtcag gctgtgaaag aagtagttca
    19021 tcgatattca cacctaattt ttgtgcatat tgcggatcta acgcatgctc agcatcaatg
    19081 aaagcggccg ttccgccatt tttttgtact tctgcaatag cgtgtagtgc aactgttgtt
    19141 ttacctgaac tttcaggtcc atatacttcg atgatacgtc cacgggggta accgccgaca
    19201 cctaaagcga catctaacgc aagagagcca cttggaattg tagagatttg ttggtcgatt
    19261 ttttcgccca atttcataat agagccttta ccatagctct tttcaatttt tttcagtgca
    19321 gcatctaagg ctgctttacg atcatctgcc aatacgataa tcctccttat atttacaaat
    19381 cagagcaaca tttcattatc cttatcatat cttttttagt gaaaaaaagc aagaaaaaac
    19441 cgaacaaata ttcgcattta tcattttctt aaaagctgtt ttctcaaaag atcaaatcct
    19501 tgcataaccg cattatatct gatagcagca cgatctctgg caaagtgata tttttccgaa
    19561 accacgccgt ctttagatga aatagcgatc catactgttc ctgcagattg accttctaac
    19621 gtgtccggtc cagccactcc ggtgaaagct acactgtaat ctgttcttgc tatctcacgt
    19681 gcttgaatgg ccatctgctc cacacattct ttgctgactg ttccatattc tgctaatagc
    19741 tccggatcaa tctccagaaa accagctttt gtttggagcg agtaggtaac gaatccgcct
    19801 ggaaatactt cagatacacc agcaatgtcg cctaatgcgg cttggaaggc gccggctgtc
    19861 aaactttccg ctgctgttac agtctgcttg ttttctttca gtagttccac tacgactttt
    19921 gctagcgaat tatcatcgcc gtatccataa aaatattccc ctactcgttc ttggattttt
    19981 tcttctaatg ctaaaagagc ttgattccct gcctgaacat catttgtttt cactgtcagt
    20041 cgtaatgtca cttcatttgg tttggcatat ggtgcgatcg ttggattgat ctgtgtctca
    20101 atcagatctt ttagctcagt gaccagtcgc gattcaccaa tcccataaaa gcgcaaaact
    20161 ttagaaatta gtttttcttc agaaggaaaa cgttcctcta gtaaaggacg tacttgttcc
    20221 acaaacattg gtttcaactc attaggcgga ccgggaagaa ggatatacgc atgtgtatct
    20281 gtttgataaa aagtccctaa agcaagccct gtacgatttg gtagaggaac cccgccttcg
    20341 ataatctggg actgttgcag attgtttttg gtcatttttc gatgggttgt ttcaaaatat
    20401 gccaataatt ttttgtatcc ttccgtattt tgtatgagag acttaccgag gtgagccgca
    20461 gttgcttctt ttgtcaggtc atcttctgtc ggtcctaatc cgccacataa gagtatgagt
    20521 tcacttcttg tttcagctag agcaatcaac tcttctaaac gctgctgatt atctcctaca
    20581 actgtttgat aataaacttc gatacctaga tcagctagct gttctgacaa gaaagtggca
    20641 ttcgtattca cgacttgtcc tagtaaaagt tctgttccga cagctattat ttctgctttc
    20701 attacttccc gctcccttat atagtatacg ctgtattttt atattttttt atttaaaaag
    20761 agggcaagga gctgtgacaa aaatcttgtc acagctcctt gttccaaata aacagtgttc
    20821 tcctgccaaa acctcggtca taagtcacaa tatacataaa acatgaaaag ctgttttttg
    20881 aaattatccc ttgtgactca gagttagacg gcaggctcac aacctctcca ctaaacgata
    20941 cttacattga tcctttgaac acaccgctgt ttttgatgaa gtaatccaca cctgaataga
    21001 tagtaaagat cagacaagca taaagcatga tttgatctac tggaataccg atcaggctga
    21061 atgggatatt attgataaac agcaagataa tagcaaccat ttgtgtagcc gtcttgactt
    21121 ttcccggcca agctgcagcc atgacttctc catgttcgac aagtagtaaa cgcaagcctg
    21181 tgacggctaa ttcacgacag acaatgatcg ctactaccca accaggggcc tttccttggt
    21241 caaccaacat gataaaagca gtcataacta gcattttatc tgccaacggg tccgcaaact
    21301 tgccaaaatt agtgacaagg cctcgcgcac gggcaatttt tccatctaac cagtctgtga
    21361 tactagctaa agcaaaaata actgcaccta ctagctgtgt gacagccagt gtggtgtctc
    21421 ctacgttaaa ttgtccccaa tctaaaggta aacctacaac caaaatgaaa atcgggatca
    21481 tacaaattct aagtacagtt aatttatttg gtaaattcac actatcgact cctttcttcc
    21541 ctattctacg tgactatcag cgaagtttcc accgatatta tttacttttc caaatgaatt
    21601 tattcaaagg agtattttta ttgaaattga tctttttgaa ttaacgaaga aggaccgcga
    21661 cacagatttg tcacgatcct tcttttttat acctacaagt aaacagatgc gtaaactaat
    21721 ttatacgatc attctgcgta ttgaagagta aggttcacat tttttggact ctgattgttt
    21781 tcccctggat taactggtac ggattctcca ttcgctttga tcgttgcatt gcttgctgca
    21841 ccaatcgtga tggtcgcatt tgttgctgtt tccggtaaca cggtactctg tgtttcattt
    21901 gctgccagtg tcccttgata aacataggca ttgtctacta gcacacctaa ccatacacga
    21961 ttgacagcag taaattcaag ggttacaggc ttggtagcgt ctgtgatatt tactgcgatg
    22021 gcactgccag aatcttctcc tgtagctatt gccattttct tttcttcact tgattttgtt
    22081 gttgattcta ctgtagaaga ctctttggtc tgcgtgcttt gctctgagga agaggactcc
    22141 tcgactgatc cttctacttt aagcgaagaa gctgtgtctc cgattattgg atcagcttga
    22201 cgatcttgcc aagtcatata gcccacgaca acgacaatcg ccaaagcaac caatcctaat
    22261 aaaatgacag gaagacttgt ccaaaagcga cttggatgcg attcttcctg atgcattgct
    22321 tttctagaag tacttactgt ttcaggctgc ggacgttcag ggaccgatcc tgtcaatttg
    22381 cttttcccat caaaaacatc gactaaatgg tctccatctt ctcctacagc ttcagcgtat
    22441 tgccgaataa atgcccgcac gtaaaattta ccaggcaatt ggtcaaaatc atcattgtcg
    22501 attgcttcca aataacgttt ttgaattttt gtaatctgct gtaattcatc taaagatatt
    22561 tttttattca ggcgtgcttg ccgtaatttt tctccgatgg tttcaaaagc cactcgttca
    22621 tcttctccag tctgtttgat tctccgaagc atctgctgtt gcaacgaaag acgttatgtt
    22681 tgggtgctgt gacaaaagtc atgtcacagc tccttttttc cgaataaaca ttgttctcct
    22741 gccaactcct cggtcacaag tcacaatatc caataaatat aagaagctgt tttttgaact
    22801 tgttccttgt gactcggagt taaacggcag gctcacgacc tctttcttaa acggtgttca
    22861 atcagctgtt cctcggtatt agctcaaaaa ccggcaaaat ataaggagat atttttccgt
//
LOCUS       DACCKU010000043        22743 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.045, whole genome shotgun sequence.
ACCESSION   DACCKU010000043 DACCKU010000000
VERSION     DACCKU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22743
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.045"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            493..1200
                     /locus_tag="IVN13_001561"
     CDS             493..1200
                     /locus_tag="IVN13_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7012056.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(1259..1903)
                     /locus_tag="IVN13_001562"
     CDS             complement(1259..1903)
                     /locus_tag="IVN13_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP7012057.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            2030..2317
                     /locus_tag="IVN13_001563"
     CDS             2030..2317
                     /locus_tag="IVN13_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012058.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            2425..2745
                     /locus_tag="IVN13_001564"
     CDS             2425..2745
                     /locus_tag="IVN13_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012059.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            2826..4139
                     /locus_tag="IVN13_001565"
     CDS             2826..4139
                     /locus_tag="IVN13_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7012060.1"
                     /translation="MNHFWGRRLLTRDIEAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEISKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            4136..4855
                     /locus_tag="IVN13_001566"
     CDS             4136..4855
                     /locus_tag="IVN13_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP7012061.1"
                     /translation="MRCSCCSKDIFQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            4983..5534
                     /gene="raiA"
                     /locus_tag="IVN13_001567"
     CDS             4983..5534
                     /gene="raiA"
                     /locus_tag="IVN13_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP7012062.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            5756..8290
                     /gene="secA"
                     /locus_tag="IVN13_001568"
     CDS             5756..8290
                     /gene="secA"
                     /locus_tag="IVN13_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP7012063.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            8416..9517
                     /gene="prfB"
                     /locus_tag="IVN13_001569"
     CDS             join(8416..8478,8480..9517)
                     /gene="prfB"
                     /locus_tag="IVN13_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP7012064.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            9622..10308
                     /gene="ftsE"
                     /locus_tag="IVN13_001570"
     CDS             9622..10308
                     /gene="ftsE"
                     /locus_tag="IVN13_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP7012065.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            10292..11185
                     /locus_tag="IVN13_001571"
     CDS             10292..11185
                     /locus_tag="IVN13_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012066.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIRPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            11337..12785
                     /gene="cls"
                     /locus_tag="IVN13_001572"
     CDS             11337..12785
                     /gene="cls"
                     /locus_tag="IVN13_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7012067.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            13122..13979
                     /locus_tag="IVN13_001573"
     CDS             13122..13979
                     /locus_tag="IVN13_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP7012068.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            14094..15014
                     /gene="pstC"
                     /locus_tag="IVN13_001574"
     CDS             14094..15014
                     /gene="pstC"
                     /locus_tag="IVN13_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP7012069.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            15014..15898
                     /gene="pstA"
                     /locus_tag="IVN13_001575"
     CDS             15014..15898
                     /gene="pstA"
                     /locus_tag="IVN13_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP7012070.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVMKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            15913..16719
                     /gene="pstB"
                     /locus_tag="IVN13_001576"
     CDS             15913..16719
                     /gene="pstB"
                     /locus_tag="IVN13_001576"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7012071.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDIHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            16735..17493
                     /locus_tag="IVN13_001577"
     CDS             16735..17493
                     /locus_tag="IVN13_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP7012072.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            17506..18183
                     /gene="phoU"
                     /locus_tag="IVN13_001578"
     CDS             17506..18183
                     /gene="phoU"
                     /locus_tag="IVN13_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP7012073.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            18406..19437
                     /locus_tag="IVN13_001579"
     CDS             18406..19437
                     /locus_tag="IVN13_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7012074.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNSDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNKSILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            19434..20201
                     /locus_tag="IVN13_001580"
     CDS             19434..20201
                     /locus_tag="IVN13_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP7012075.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALIVLPLTCVTTAYLFTKKKEQLKME"
     gene            20301..21869
                     /gene="liaX"
                     /locus_tag="IVN13_001581"
     CDS             20301..21869
                     /gene="liaX"
                     /locus_tag="IVN13_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP7012076.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDKASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQALEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            22048..22353
                     /locus_tag="IVN13_001582"
     CDS             22048..22353
                     /locus_tag="IVN13_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP7012077.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            22353..22703
                     /locus_tag="IVN13_001583"
     CDS             22353..22703
                     /locus_tag="IVN13_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP7012078.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         7463 a         3921 c         4908 g         6451 t
ORIGIN      
        1 gttagttcga gagctctttt ttttgctttg ctaccttgtt ccttgaagat tctgctgaat
       61 taagatagag ttaagttaac tacaagaatt ggagggatcc acatgaaatg gaaagaaaaa
      121 gtagacgaag cagctgaaaa gttatatgat ttggtcaaat cagaaaaata caatattgaa
      181 gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa aatcaaaacg cccaacgaat
      241 catacaaaaa aatggatggc taaaaaccgc taataataac aaaagatcca taagtcggca
      301 ttttcatcga ttttagggtc ttttgttatt ttctaggttg aacatatcta aaaaattgtt
      361 tctgacaata ggagtaggga gaacgatgtg tgtctaaatc ctctgttgat ggatagaaac
      421 ctttatcttt ttcagttgtg aatcaggtat ataccagtta taatagaaga aacgaaagga
      481 cgggtgtacg ttatgtcgaa tcaattacct gtatatatac agattcatga ccaattaaaa
      541 aatgaaatcg aacagggtat atggaaagtc ggagaccgtc ttccttcaga aagagaatta
      601 gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag caattcaaaa cttggcagat
      661 gaaggaatct tagaaagaaa gatcggttcg ggtacgtatg ttgcgcgaga aaaagtacaa
      721 gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg aatcacaaaa tcgagtaccg
      781 tctagtcgga caatctctta tttcttgact tcgccaagct caagtgaaat ggaaaaacta
      841 aacttgagta aagatgacac gatcttgcga atggaacgta ttcgtttcgc agatgatgtc
      901 cctatttgtt ttgaagttgc cagtgttcca caaaagctga tcgatggcta cagcaaagca
      961 gagattactg aatcgttgta tcgcacgcta gaagaaaaag gcgggaaaaa gatcggtcca
     1021 gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa ttgctgacta tttagaggta
     1081 aaacgaggag atgcagttct tcgtttgcgc cagatttctt attttgaaga cggtacgccg
     1141 tttgagtatg tgcgtacgca atacgtagga aatcgttttg agttttattt ggaaaaataa
     1201 aaaacaggtg atccctgatt ccttattaga aataaaagag aatcagaatc acctgttttt
     1261 atatatgatc agttatcagt tgctcgtgat aagaaacaga accctcttca aattggactt
     1321 gtccgttcag cagctcaatg atggattctt taaagtggtc agcacttttt tcatctacag
     1381 cacagatgac gctaacttga tcagtatatt ggatttcttt tataaaaaaa tgttggagtt
     1441 caagataatt ctgtaatttt cctagttgcg gatacgtgat catcacagag atctcctgtt
     1501 gcaaggtacc ttctacgatt ccaatgtcag acaatgcttg agagaccgca tgagtgtatg
     1561 ctcgtattaa tccgcctgtg ccgagtttcg ttcctccaaa atatcgagtg actactgcag
     1621 ctacattgat cagttgattt tttttgagga cttccaacat cggtacgccc gccgttccac
     1681 ttggttctcc atcatcgctg cttcgctgga tttcattttt ttctccaatg atgaaggcgc
     1741 tgcagttatg gttcgctttc caatgttctt tttttatttc ttgaatgaaa ctttttgctt
     1801 ggtcctctga ttctattcgt ctaagagaac agataaagcg tgattttttg atctcaatct
     1861 cactttgtcc attttctttt attgtacgat agcgctcgat catcttgtca cctctttttt
     1921 tcattttctt tattcagtat aaataaagtt ctaggttgtt tcaacactgt aacggttgcg
     1981 caattgctgt agtatgcgat aataaagaca aaggcaggtg gatctgggga tgcaggaacg
     2041 aatgaaaaag tacgatgcaa ttacgcatta tttgaagaac aatggcggct ctcaagtaac
     2101 gttgaccttt acccaatttg acgaactgct gtttcccagt aacggacttc ccaaaacagc
     2161 aagggaaagt acagattggt gggcaaatga ttataaacac cctgaaaaag gtgcctatgg
     2221 ctggataaat gcaggctatg aagtagttgt catcaatttg gacaaagaat atgtcgtgtt
     2281 caataaatta gtgaaatcta gttggctatt tgattaaata aaccgcgttt agaaaacgtt
     2341 gacaaatttc tttcttttta tataatagag taacagacgc gaagaaaacg tttgcatagt
     2401 ttgttttaaa aaggagagaa aagtatggct aaaaaaacaa tcatgcttgt atgttcggct
     2461 ggtatgagca caagtttact agtaacaaaa atgcaaaaag ctgctgaagc aaaaggaatc
     2521 gactcggata ttttcgctgt gtccgcttct gacgcagata acaaccttgc aaataaagac
     2581 gtggatgtgt tgttattagg cccacaagtt cgctttatga aagctgattt tgagaaacgt
     2641 ctggaaccaa aaggcattcc tttagatgtc atcaatatgg ctgattatgg tatgatgaat
     2701 ggcgaaaaag tgttagatca agcaatcacg ctcataggtg aataaataga acagtataac
     2761 tatcgaacaa gcgatccaaa aaatagatcg cttgtttttt cgtatatttt tttagggcga
     2821 agaatatgaa tcatttttgg ggtagaaggc ttctgacgag agatattgaa gcaatagact
     2881 gtgaggaggt aaatttacca aattatgaga agatcgcagc agtggaacgt ttgggcaact
     2941 ggattctttg tcatcgctgt ggaatgagga cgtccgtcat tgatggacag cttgcagatt
     3001 acggttattt ttgtacacat tgtctatatt tagggcgatg cgacactcaa caagatttat
     3061 atttgtttga tcagcctgaa gcaaaacctc gagaagtcgt ctttttttgg acaggacagc
     3121 ttactgaaaa acaaacggaa atctcaaaga gaatactttg ccattcagaa aaaagacatc
     3181 atcttatttg ggctgtaaca ggtgcaggga aaacagagat gctttatcct attttagtgg
     3241 aaactttaaa agctggagga agagtagcga tttgtacacc gcgagtcgat gtttgtaatg
     3301 aattgttttt acgctatcgt tatgtttttc cagaagaaaa aatcacgttg ctccatggga
     3361 atacagcaac ggcttatacc tttacttctt ttgttatctg tacgattcat cagctatacc
     3421 gtttttatcg gtcctttgat ttaatagtga tcgatgagat cgatgcgttt ccttataacg
     3481 gggatgcagg attagctcat gctgtgcaaa cagcgattaa accagacgga cgattcatct
     3541 acttaagtgc cacacctgat aaaaagttac taaaggaaat caagcagacg tttgacatcc
     3601 acaaacttgc tttacgcttt catcggcgtt tgcttcctga acccgtcttg gttttcatga
     3661 ataattggcg acaaaaatgc aagaacaaga agaagataaa atctttggtg cggatcattc
     3721 atcaattaat taaagaaaac catatccttt tattttgtcc aagtatcacg atgatgcatc
     3781 gtttgaaagc cacacttcaa gaggctcttc cgagatatga gatcacagat gtttcttcaa
     3841 gagacaatca gcgggcggaa aaagtccaga aaatgcgaga agcaaaatat gatatactgt
     3901 taaccacgat gatccttgaa cgtggagtca catttgaaag aatatccgtt atcgttttag
     3961 gagcggatca tccggttttt actaaatctt ctttggtaca aatcgcagga agagccgatc
     4021 gtaaagggcc ttatacgaac agtcaagtat actttttcta tgaagaaaaa actgcagcta
     4081 ttcgaaaagc ttgtcaggaa atcaaagaaa tgaatgaaga agggaagaag atgctgtgag
     4141 gtgcagttgc tgcagtaaag atatcttcca aaatttaaca ttcagcgaga ttattggtgt
     4201 ttcgaaatac agattacctc agacttgttc agtttgtcgc gcacaatttc atttattgga
     4261 aacaagaggc tgttctggct gtcaaaaacc aatccatttc aatctagaga gtacgaaaaa
     4321 gaaagtaaag tctattcccg tttactgcag tgactgtcaa gaatggaaga agaaatatcc
     4381 gacctatgat tttcagcacc gtgccttttt tgtgtacgat gaaaatatga aggcctggct
     4441 caatcaatac aagtttatgg gagatatccg actagcaggc acctttgcat ataaatggac
     4501 gcagataaaa aaagaatacc cgacatacat ctattgtcca atacctttat ctgaaaaaag
     4561 gcgaacagaa agaggattca atcaagtaag cgagatgttg aaagcagcaa gaattccttt
     4621 tcagatgcta ctcaaaagag agaagcatct gctggctcaa gcaaaaaaca cgcgaaagga
     4681 aagattggag atgcctcaac cttttgaact tgcagtcacc ttttctaaga tacaaaataa
     4741 aaaaatattg attattgatg atgtctatac aacaggacag actcttttcc acgcagcaga
     4801 ttgtttgcag gtcgcacagc cgaaaatcat ccgaaccatt tctttggcga gatgaattag
     4861 atgtggaaat ttgatagcga tttaatttgc aaataaccta gagttcgata taatagaatt
     4921 aagaagaggg aaagagtggt ccttctcctt cttgattgtg gtagacgaaa ggggtaattt
     4981 ttatgtttag atataatgta cgcggcgaaa atatcgaagt aactgaagcg attcgcgact
     5041 acgttgagaa aaaagtaggc aaactagaac gctatttcag tgacgcaccc gatgcaacgg
     5101 cgcatgtgaa cttaaaagtt tatacagaaa aaacagcaaa agtggaagta acgatcccat
     5161 taccttatct tgtattacgt gccgaagaaa cttctccaga tttatacgca agtatcgatt
     5221 tggttgttga taaattggaa cgccaaatcc gcaaattcaa aacaaaaatt aatcgtaaat
     5281 cccgtgaaac gggcatgaat actgcagatg ctgctgcttt attcaatgaa gaggacgaaa
     5341 acgaaaatga ttctgaatta gatatcgtac gtacgaaacg cctttcattg aaaccaatgg
     5401 atagtgaaga agcagtctta caaatgaaca tgctgggaca taacttcttt atttttgaag
     5461 atgcagaaac aaatggtaca agtatcgttt accgccgtaa agatggaaaa tacggactga
     5521 ttgaaacaga ctgatcagcc ttacttaaat aacaacgaaa acttgcagca gctactgaca
     5581 gctgccgcaa gtttttttga gtttcctcct cttttagatg aaatatgaat aaattatgaa
     5641 gttcttactt taatacgctt aagatatttc aattatctat aactaatgat aaaatgaaaa
     5701 gtaaagtcta catagtctgg ataatacaca gaaggaaaag gagattacac aacgcatggc
     5761 caatttgatt cgttcactta tcgaaaacga taagaaagaa ctaaaacgtt tagatagcat
     5821 tgcaaaaaaa gttgaagcat acgctgacca aatggcagca ttaactgatg aacaattaca
     5881 ggcaaaaact gctgaattca agggcagata ccaaaaagga gaaacattag atcaattact
     5941 tccggaagct tttgcagtcg tccgagaagc agcaaaacgt gtattggggt tatatccata
     6001 ccacgtacaa ttgatgggtg gaatcgtgct tcacgatgga aatatccctg aaatgcgtac
     6061 tggtgaaggt aaaaccttga ccgcaacgat gccagtatat ctaaatgctc tgactggcga
     6121 aggggtacac gtagttaccg tcaatgaata tttagcaacc cgtgactcga cggaaatggg
     6181 cgaactttat aatttcttag gattaacagt ggggttgaat atcaactcaa aatcttcgga
     6241 tgaaaaacgc gaggcttata attgtgatat cacttatagt acgaataatg aattaggatt
     6301 tgactacttg agagacaata tggtcgttta ccgccatcaa atggtacaac gtccgctgaa
     6361 ttatgccatt gtcgatgaag ttgactcaat cttgattgat gaagcgagaa cgccgctgat
     6421 catttcaggg caagcagaaa aatccacagc tttatataca agagcagaca acttcgtgaa
     6481 acgtttgaaa gaagaagaag attataaaat cgatgtacaa tcaaaaacaa tcagtttaac
     6541 cgaagcaggg atcgaaaaag cagaagaaac attcggtttg gataacttat acgacattga
     6601 aaatactgca ttgacccatc acttggatca agcattgaga gccaactata tcatgctttt
     6661 agatatcgat tatgttgtcc aagacggaaa agtactgatt gttgaccagt tcactggtcg
     6721 gatcatggat ggacgccgtt attcagatgg acttcaccaa gcaatcgaag ctaaagaagg
     6781 cgtagagatc gaagacgaaa cgaaaaccat ggcgacgatc actttccaga actatttccg
     6841 tatgtacaag aaacttgcgg gaatgactgg tacagctaaa acagaagaag aagaattcag
     6901 agaaatttat aacatccaag tataccagat tccgacaaat cgtccggtta tccgtgatga
     6961 tcgtccagat ttactttacc caacattaca aagcaagttc cacgctgtcg tgcaagatat
     7021 caaagatcgc taccgcaaag gtcaacctgt gttagtcgga actgttgcgg ttgaaacatc
     7081 tgagttatta tcagatatgc tgaatcaaga acgtattcct catgaagtgc tgaatgcgaa
     7141 aaatcacttt aaagaagcag agatcatcat gaatgctggt caaaaaggtg ctgtgacgat
     7201 tgctaccaac atggctggtc ggggaacaga tatcaagtta ggacttgggg taagagaact
     7261 tggtggtcta gcagttatcg ggacagaacg acatgaatct cgtcgtatcg ataaccagct
     7321 tcgaggacgt gcaggacgtc aaggagaccc tggtatgtca caattctact tgtcattgga
     7381 agatgatttg atgaaacgat tcggttcaga acgaatcaaa gctttcttag atcggatgaa
     7441 gatcggtgag gaagatgctg ttatccaaag caagatgctg acgaaacaag tagaatctgc
     7501 acaaaaacgt gtagaaggaa ataactatga cacacgtaaa aacgtcttac aatacgatga
     7561 tgtgatgcgt gaacaacgtg aagtaatcta taaacagcga caagaagtaa tcatggaaga
     7621 aaaaagctta tccaagccac taatgaacat ggtcaaacgg acaatcagtc gtgtagtaga
     7681 tgcgcataca caattagaga aagaaaattg gaatttggaa agtatcatcg attttgccgg
     7741 cagtactctt gtccatgaag atagtattac tcttgcagat atcgaaggaa aaacatctca
     7801 ggagatcaaa gagtatctaa acgaacgagc acaagaagta tttaatacca aagcggctca
     7861 actaaatggt ccagagcaac tgttggaatt tgaaaaagtc gtgatcttgc gtgttgtgga
     7921 ctctaaatgg acagaccata ttgatgcaat ggatcaatta cgtcagtcaa tcggcttacg
     7981 tgcttatgga cagaacaatc cattagtcga atatcaaaca gaaggctata agatgtttga
     8041 agatatggta ggcgccatcg agtatgaagt cactcgaatc tttatgaaag ctgagatccg
     8101 tcaaaacgta cagcgtgaac aggtttctca aggaacagct tctcactcag aagacggaga
     8161 tgccaaagaa aatagtaata ctagcgcgaa aaaacagcca atccatgtga acaaggtcgg
     8221 aagaaatgat ccttgtcctt gtggaagcgg gaaaaaatat aagaactgtc atggaaaaaa
     8281 tgcaaactaa tttctgacag caggattggt gagacaaatt ctttttttaa gaattcgtct
     8341 caccttcttt ctgttatttg ctgattctgt tagactagat ataatgaaaa aagtatagtg
     8401 aaagaaggaa aaaacatgga aatatccgaa attcgaaatc tcctgacaga aatgcaggaa
     8461 aaggtcacaa gtttcaggag gtctctttga cttagatcag ctggaagaag acatcgcgga
     8521 agcagaagca aaaatggctg aacctggttt ttgggatgac agcgaagccg cacaaaaagt
     8581 gatcagcgaa aataacgcta ataaagaaac ctatgaccaa ttccattctc ttgccgaaga
     8641 actagaagaa atcgaattga tggtggagat gctgcaagaa gaacctgatg cagcaatgca
     8701 agaagaagca gaaacacgta tcaatgcatt agatgaaaaa atgaagacgt atgaattatc
     8761 tatgctgtta gacggacctt atgatcgaaa caatgcgatc gtcgaattgc atccaggggc
     8821 tggcggaacg gagtcccaag actggggcag catgctttta cgtatgtata cacgttgggc
     8881 agagcagcat ggctatcaag tagaaacact agattatcaa gcaggtgatg aagcagggat
     8941 caagagtgtg accctgctga ttaaaggaca taacgctttt ggttatctaa aatcagaaaa
     9001 aggtgttcat cgccttgtac ggatttcgcc ttttgactca gcgaaacgac gtcatacttc
     9061 tttttgttcg gtagatgtga tgccagagct tgatgaaacg atcgatatag aaatcaatcc
     9121 agatgatttg aaaatcgata cgtaccgagc aagtggtgca ggtggtcagc acatcaataa
     9181 aacagaatct gctgtacgga tcactcactt gcctacagga acagtagtag ctagtcaggc
     9241 acaacgctca caattgaaaa accgtgagca agcgatgggg atgttaaaag ctaagcttta
     9301 tcaattagaa gtggaaaaga aagaacaaga agcagctgca cttcgaggag aacaaaaaga
     9361 aatcggctgg ggttcacaga tccgttctta tgttttccat ccctattcta tggtaaaaga
     9421 ccatcggaca aacgtagaga caggaaatgt ccaagcagtt atggacggtg accttgattt
     9481 atttattgac ggctatttaa aaatgcatct acaataagaa ctcattgtta aattgaaata
     9541 aaattgtaag attttgcaag catgttgaaa cattgccatg ctataatagc taaagactga
     9601 aaaagatatg gagagaacgc catgattgaa atgaaggatg taatgaagaa gtactcaaat
     9661 ggtacgaccg ctatccgcaa tctctcgatt gtcatcgaac aaggagagtt tgtctatgtg
     9721 gttggtccgt ctggagccgg taaatctaca ttcatcaaat tgatgtatcg agaagaaaaa
     9781 gcttcaaaag gaactttgaa cgtcgcaggt catgatctaa tagcaatcaa aaaccgcgaa
     9841 gttccttatc tacgcagaga aatcggcgtg gtgttccaag actataaatt attaccaaga
     9901 aaaacagtat atgaaaatgt tgcgtacgct atgcaagtta tcggcagaaa acctcgagat
     9961 atcaaaaaac gagtaatgga agtgctggac cttgttggtt tgaaacacaa agttcgagtc
    10021 tttcctagcg aattatccgg tggggaacag caacgtgtct ctattgctcg ggcaatcgtg
    10081 aatacgccta aagtacttat cgcggatgaa ccgacaggaa atctggatcc agaaaactca
    10141 tgggaaatca tgaaattatt agatcggatc aatgcccaag gaactactgt cgtgatggct
    10201 acacataaca gtacgatcgt aaatacgatt cggcaccgag taattgctat cgaaaacggc
    10261 cgcattattc gagatcaagc ggaaggggaa tatggatacg atgattagaa cctttttccg
    10321 ccatttatta gaaagtatca agagtttgaa acgtaacggc tggatgacag ttgcttcagt
    10381 cagtgcggta acgatcacgt tgactttagt cggtgtattc atggcagtta tcatgaatgc
    10441 aactaaatta gctcaggata tagaaggaaa cgttgatgta tctgtattcg tagatatcgg
    10501 tacaaaagac gaagagatga aaaagctaga aaaagaattg aatgacttag atcatgttaa
    10561 aaaagttgaa ttttcaagca aagaacagga attgaaaaaa atccaaaacg aaatgggtga
    10621 ttcctggaaa ttgtttgaag gtgacaataa cccgttatat gatgtgtatg tagtaagtgc
    10681 tactgatcct tcttatacaa aacaagtagc agaagaagca gcagatttga agaatgtttc
    10741 tcgttctgat tacggtggag cttcctcaga tcggattttc caaatcgctg gtgcagttcg
    10801 gacatgggga ttagttgcag cggcattgct gctgttcgta gcgatgtttc tcatttcgaa
    10861 cacgattcgg atcacgattt tgtctagaca gcgtgaaatt caaatcatgc gtttagtcgg
    10921 tgcaaaaaat ggatatattc gctggccgtt tttccttgaa ggtggctgga tcggtctgat
    10981 cggtgcgatt cttccgattt tgatcatcac atttggttat gcatggtttt tccaattgat
    11041 caatccatca ttattacgtt cccattattc attgattcgt ccacaaaata tcgtttggaa
    11101 aatcaatctt ttaatggtcg gcattggtgt gatcattggt tcacttggct cgatcatttc
    11161 tatgagacgt tttttgaaat tctaatacca caatataaat aaaatgagaa agttggacag
    11221 tttcgttcca gctttctttt tttattggct aggagaatag aaggaagcgg cttttttccc
    11281 tttactttaa aaaacaactt gggtattctt aagttggtag aagaagaggt gaagccgtga
    11341 aaattatcgt tgacaatttt tttacgatcc ttttgattat gaatattttg ctttccttta
    11401 tcatcgtttt tcgagaaaga aaggaaactg ctcaaacatg ggcatggctg ctcgttttga
    11461 tgttcattcc agttgtagga tttattttat atattttttt aggaagaggg atttccaaag
    11521 agaaaatctt tgatttgaag atccaaggga aaatcggtaa gaacctggaa attgaagaag
    11581 acaaacaggc attgatgcga gggctttatc ctcatccgcc tactggacaa gtagatgtga
    11641 aacaattgat ctatatgctc acggtatttg aaagtacgct ctatacgact aaaaatgaaa
    11701 tcactttatt tacagatggt cgtgaaaaat tcgatgcgct gattgaagat atcaagcagg
    11761 cagaagacca tatccacttc cagtattata tttatcgcag tgatgcatta ggtgaagaag
    11821 ttcgagatgc attgacagag gcggcaagac gaggggtaaa agtcagagtc cttcttgatg
    11881 catggggttc cacacaagtt tcttccagct tttttcagaa tttgaaaaaa gcaggcggag
    11941 aagtcgcatt tttctttcct ctatttgttc cgtatattaa tcctcggatc aattatcgca
    12001 atcatcgtaa aatcgtagtt attgatggga aaatcggata tacaggtggg ttcaatgttg
    12061 gaaatgaata tctaggattg gtaaagaaat ttggttattg gcgagacaat catttgcgta
    12121 tttacggaga agcggtgtat accttgcaaa accgtttttt gatggactgg aattctcagc
    12181 atacaaaaga agtaggctat tctcccaaat ttttcccgtc cattcacact actggtgata
    12241 tagctgttca aatcgtgaca agcggaccag ataccgaaca tgaacaaatc aaaatgactt
    12301 atttgaaaat gatttcgtta gccaaacggg agatattgat ccaaacgcct tattacatac
    12361 cggatggatc gattcatgaa gcactgaagt tggctcttct ttcaggcgta aaagttcata
    12421 tccaaatacc taacaaacca gatcatcttc tcgtttactg ggcaacgtat tctttcgctg
    12481 cagagctgat cgagtatgga gcaaggatcg aaacatatga gaatggattt attcatgcaa
    12541 agacgatgat tatcgatggt ggcattgttt cagttggctc agcaaatatc gatgttcgtt
    12601 catttcgctt ggactttgag gtcaatacac tggtttatga tgaacgaatg gcttcacgtg
    12661 taagaaaagc tttttttgaa gactcgaaaa tttctacgca tctcacaaaa gaaatgtatg
    12721 aaaaccgagg aataatcatc aagatgaaag aaggattagc aagattgatt tcgccgctcc
    12781 tttaaaacat aaaaaggaca tgagaaccat caacagatgg agatctcgtg tccttttcta
    12841 acggggccat ttctgagagt acgtcagaaa tggagaaagc gtctcgcatg agcgaaatta
    12901 actttggggt atctcttgtt ttttggggaa agcaagaaat aagctaatat atgctattaa
    12961 aagagacaca tttcaaagcc agtatagcat agaaactttt aatgagatag ttttttttac
    13021 aattatgaga atttattttg ttatttaatg attttgattc gttttcatca agtaatgaaa
    13081 atgataaaat aaacatactt taggattaaa ggagatttct aatgaaaaaa cttaaattat
    13141 tcttaccaat aataggatta gccattcttt tatcaggctg ttcaaagtgg attgatcgag
    13201 gagaatccat tacggctgtt ggctcttctg ctttacagcc tttagtagaa acagttgctg
    13261 aaacttacca gacagaaaat cctggaaaat tcatcaatgt acaagggggc ggaagcggaa
    13321 ctggtttgtc tcaagtacag tcaggagcag tcgacatcgg aaactctgat ttgtttgcgg
    13381 aagaaaaatc ggggatcgat gcctcagcat tagtcgatca tcgagtagca gttgttggga
    13441 tcactcctat tgtgaacaag gatattggcg taaaagacat cagtatggaa aacctgaaaa
    13501 aactatttac aggcgagatc aaaaactgga gtgaagtcgg tggaaaagat cagccagtag
    13561 ttatattgaa ccgcgcttca ggaagcggaa cccgtagcac gtttgaaaaa tgggtattgg
    13621 acggagaaac agctgttcag gcacaagaac aagattcaag cggaatggtg cgccagatcg
    13681 ttgccgacac acccggtgca atcagctatg ttgctttttc ttatgtttcc gatgatgtag
    13741 atacgttgaa tgttaacggt gtagaaccga cagatgaaaa tgtgacaacg aacaaatgga
    13801 cgatttgggc atatgagcat atgtacacaa gagatcatcc aaaaaaactg acaaaagaat
    13861 ttttagacta tatcttatct gatgaagtac aagataacat cgttggagaa ctaggttata
    13921 tccctgtttc tcatatgaaa gtcgaacgtg attggcaagg aaatatcgtt aaagagtaat
    13981 ctttacacaa aatttacaaa cttagctaaa cttttacatg ttattaaaac caaattgatg
    14041 ttcaatttgt acactgagtg agtatgttaa acctgattaa gggggagtca tctgtggaag
    14101 atgtgaaaaa agtattatta acaaaatcaa aacgttcaaa aatggaacag cgtggaaaat
    14161 tcatcagttt tttatgtatc gcgctgatcg tgttggttgt attatctatt ttttattttg
    14221 tagctagtaa aggattggcc acctttttta ctgataagat caatgtattt gattttctgt
    14281 ttggtacaga ttggaatcca agccaaatcg gagcaaatgg gaaaccaatg gtaggggcat
    14341 tgcctatgat cacaggttcg tttattgtga cctttttatc tgctatcgtt gccacgccat
    14401 ttgccattgg tgcagcagta ttcatgacgg agatctctcc taaaaacgga acgaagatct
    14461 tacagccggt catcgagctg cttgtaggta ttccttctgt cgtttatggt tttatcggtt
    14521 tatcagttat cgtgcctttt gtcagaagta tttttggcgg gacagggttt ggtatcttag
    14581 ctggtacatt tgtgttgttc gttatgatct tgccgactgt cacaacgatg actgttgatg
    14641 cattaaaggc agtaccacgg cactatcggg aagcttcatt agcgcttggt gccacaagat
    14701 ggcaaacaat ctacaaagtc gtgctacgag cagccgtgcc cgggatttta acagcagtcg
    14761 tttttggtat ggctcgtgcg tttggtgaag ccttagctat ccaaatggtc atcggtaatg
    14821 cagcattgat gccaacagga cttactactc ctgcttctac attgacgtcc atcttaacaa
    14881 tgggaatcgg aaatacaatc atgggaacag ttgaaaataa tgtgttatgg tcattagcgc
    14941 tgatcttgct attgatgtca ttattcttta atatcgtgat ccgtatgatt gggaagaaag
    15001 gagagctgaa ataatgaacg caaaacgctc agataaaatt gctacaggtg ttttatatat
    15061 agtttcgggg atcatcgtat tgattttagc agctttacta ctttatatct tagttcgggg
    15121 aataccgcac atttcatggt ccttcttgac cgaaccttcc aaagcctacc aagaaggtgg
    15181 tggaatcggg atccagctat ttaactcgat ttatttgctt ttgatcacga tgatcatcag
    15241 ttttcctatt tctttaggtg cgggtatcta tctttcagaa tatgctggaa aaaactggct
    15301 aacagatgtg attcgcacgt cgattgaaat cttgagctcg ttgccttctg tcgttgtcgg
    15361 tttattcggt ttcttggttt tcgttattca attccagtat ggtttctcga ttatttctgg
    15421 tgcattggct ttgactgtat tcaacttgcc tcttttgact agaacagttg aggagtcttt
    15481 acaagcggtc cattacacac aacgagaagc aggattggcc ttaggtttgt cacgctggga
    15541 aacagtgatg aaagtggtgg ttccagaagc gacaccaggg attttgactg gtgtgatcct
    15601 aagttctgga agaatttttg gtgaagcagc tgcattgatc tatacggctg gacaaagtgc
    15661 acctgcgtta gattttacga actggaatcc attaagtgtt tcaagcccga tcagcatttt
    15721 ccgtcaagct gaaacattag ctgtccatat ttggaagatc aatacagaag gcacgatgcc
    15781 tgatggtgtt tctgtttcgg ctggcgcttc tgccgtgttg atcattgctg ttttactgtt
    15841 caacttcggc gctcgtgcga ttggcaaacg attataccga aaaatgactt cagcctaagg
    15901 agaagaagca aaatgaaaaa atataactgg aatgaaacaa atgtcatcac catgccaaaa
    15961 gagaaagaag tagcactata tacggatgat atacatgtct ggtatggcga caatgaagca
    16021 atcaaaggag tggatctgga atttgaaaaa aataagatca ctgcactgat tggtccttcc
    16081 ggctgcggaa aatctacgta tcttcgttcg ttgaatcgaa tgaatgatgg gatcgctaat
    16141 acgaaaatca ctgggaaaat catgtatcaa ggcgtagata ttaatgcgcc tcaagtggat
    16201 gtttatgaga tgcgcaaacg gattggcatg gtttttcaac gtcctaatcc gttcagcaag
    16261 tcgatttatg acaatattac atttgctttg aaacagcacg gcgaaaaaaa caaaaagaaa
    16321 ttagatgaaa tcgttgaaac aagcttgaaa caagctgcct tgtgggacca agtaaaagac
    16381 agcttggaca aaagtgcctt ggctttatct ggcgggcaac agcaacgttt atgcattgct
    16441 cgtgcgatcg cgatgaaacc tgatatcttg ctgctagacg agccggcaag tgctttggac
    16501 cctatttcga caggaacggt tgaagaaaca cttgtaaact tgaaagaaga ctatacgatc
    16561 atcatcgtga cgcataacat gcaacaagca gctcgtatca gcgactatac tgcctttttc
    16621 tatctaggaa aagtaatgga atatgacaaa actaacaaga tatttaccag accaaaaatt
    16681 caggcaaccg aagattatgt gtccggtcat tttggttaag acggaggcct caacatgaca
    16741 aaagaaatta tttcatctaa agatcttcat ctgtattatg gaaagaaaga agcactaaaa
    16801 gggatcgatc taagtttcaa tcaaggggaa atcactgcga tgatcggtcc ttctggttgc
    16861 ggaaaatcca cttatctgcg ttctttgaac cggatgaatg acctgattcc gaatgtaacg
    16921 atcaccggaa gtgttttata taaagacaaa gacatctatg gtccaaaaac agatgttgtt
    16981 gaactaagaa aagaaatcgg catggtattc cagcagccaa acccatttcc tttttctatc
    17041 tatgaaaatg tcatctatgg attgaaatta aaaggggaaa aagataaaaa agtccttgat
    17101 caagtagttg aagaaagcct gaaggcagcg tctgtttggg atgatgtgaa agacaaattg
    17161 cataaaagtg ctttatcttt atccggcggg cagcagcagc gtgtatgtat cgcacgtgtt
    17221 ttggcagtta atccggaaat catcttattg gatgaaccaa caagtgcgct tgatcctgtt
    17281 tctactggga aaatcgaaag tatgctgtta gaattaagag agcggtatac gatgatcatc
    17341 gtgacccata atatgtctca agcttcacgt atttcagata agacggcatt ctttttagat
    17401 ggtcacttga tcgaatacaa cgatacgaag aagatattca tgaatccaga aaaacaagag
    17461 acagaagatt atatttcagg tcgatttgga taaggaggaa acggaatgct acgtacacaa
    17521 tttgaagaag aattgctgaa tctccataat cagttctacg aaatggggat gatggtgagc
    17581 agcgccgtgc acaagtcagt tcgtgcatac atcaaacacg ataaaatctt agcccaagaa
    17641 gttatcgaaa acgatataaa tatcaataac atggaaacac gcttggagaa gaaaagtttt
    17701 gaaatgatcg cattgcaaca gccagttaca actgatttgc gtatgatcat tacggtcatg
    17761 aaagctagct cggatttaga acgtatggcc gatcatgcgg tgtctgttgc taaatccacg
    17821 attcgcgtaa aagggcagac gagaataccg gaaatcgaaa aagagatctc cgatatgtcc
    17881 gattatgtga aaaaaatggt ggacaacgtc ctagttgctt atgtcaaaac agacgaaaaa
    17941 gacgcacgta cgatcgccaa tatggatcaa agagtcaatg actatttcaa tcgaatctat
    18001 aatgcaacaa tcaaaaatat gcaggaaaat cctgagacag tcatcagtgg aacagactac
    18061 ttgaatgttg ccagttatct agaacggatc ggagactatg tgacaaacat ctgtgaatgg
    18121 atcgtctact tggctacagg gaaaatcact gagttaaata cgaaccacaa tgagattttc
    18181 taaactgaag ctgtgattat gccgtttgtt tatgaaaaga ggttgtgaaa aagctgttct
    18241 acatcaagta ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg
    18301 acttattacc gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa
    18361 aacatgtcac aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt
    18421 agcatcatca ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagcc
    18481 gctttatatg aagtacctgt catcgagata gaaaaagtac gaataaaaga ggatcatcaa
    18541 ttgattacag gaaaactgat gaactcggat caaaaaggag aatcgattta tttatcggtt
    18601 ccttatcaaa aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc
    18661 caaatgcatg gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc
    18721 ttcacaagta tcttcattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct
    18781 ttaggaagtc tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa
    18841 acagaaggga tttctctatt aaataaatct atcctgttcg tcgttatagg gatgattatt
    18901 agcatattgg ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt
    18961 acgagtgttt ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa
    19021 ggactgcgct atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct
    19081 agcgtactga ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca
    19141 ttagaagaat tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga
    19201 aggcaagtgg gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc
    19261 agtgggtcag ttccatcgct tcttttatat ttaggaaatc aatggtcttt caaagaaacg
    19321 atagaacagc atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta
    19381 ttatcgatcc ccatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg
    19441 tacttattat acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag
    19501 gtatgtatat tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg
    19561 gttatcatca gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg
    19621 tggttatctt attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta
    19681 tactgatctt tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc
    19741 aaggttttac atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg
    19801 attttagatt acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg
    19861 cgagtgtagc aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt
    19921 tttaccaatt gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa
    19981 tgacacttct ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt
    20041 tagacattcc ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg
    20101 ctattcttac cacattgtcc gcactaattg ttcttccttt aacctgcgtg actacagcat
    20161 atctctttac aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct
    20221 atgacatttt tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt
    20281 tgcttaagga ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag
    20341 gaatcctttc gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg
    20401 aaaagcaaat caaaaaagaa gcagacaagg taactgctag tcataaagaa aaagataagg
    20461 caagtcaatt gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc
    20521 caaaggaaag agaaagacaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa
    20581 ctgaagcgaa taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata
    20641 aggcagcacg caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag
    20701 gcgagttgac agaagaagaa ttagcacagc gtcaggcgtt agaggcagaa atcaaagagt
    20761 tagaagctag tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag
    20821 atatccgtaa gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc
    20881 cagatgattg gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag
    20941 aagctggatc acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag
    21001 tgaatgacaa catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga
    21061 aatttgagca tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag
    21121 caaacggaaa cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga
    21181 tcaaacttta cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga
    21241 ttgaggttaa tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc
    21301 ttgttttcta cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca
    21361 acatcatgat tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca
    21421 tcatcgtgga ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg
    21481 atattcgaaa cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat
    21541 ttggtgcgac agcagaaaat ctttctgtgt ctttagtaaa tggtgatgta cgtcttacga
    21601 ttaaagaaga taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg
    21661 ctttaccaga tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaata
    21721 gccgtctgtc caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc
    21781 aattccgtcg tgtttcagat gatgagatcg cgcaagtgca actttcaacg acaacaggaa
    21841 gtatctattt aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag
    21901 caataagaag cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc
    21961 gataggctat ctcttgaaca ccgattaaga agaaggttgt cacaacgatt ttcttcattt
    22021 aaaatagtag aacagaggtg agacatgatg aaaaaaaagt tgacaaaatc caataaaaat
    22081 gttgttttga caggtacttt agcagggatt ggggaatata tcgggattga tcccactgtt
    22141 atccgtgtgc tttatgtatt cctaagctta gttgcttttg gaagtccaat cattttatac
    22201 attttattag ctttgattat cccatctgcg cgttctgcac gaacaaacta tggacataac
    22261 aatgcatatt atgaaaaaaa caattatcgt gaacaaacaa aaaaacgcaa agaagcggaa
    22321 aaaatcgatg atgacgaatg gagtgacttt taatgtcgta ctttcagcgt attttggtta
    22381 atacgttaac attcatatcg ttggctgttc tttttcctgc gatggtccat gtgagcagta
    22441 tttggacagc cgtccttgcc agcttcgtat tatctgtgtt gaacatgttt gtcaaaccga
    22501 ttctgacgat cctgtccctg ccctttacat tagtcacatt gggattgttt acgtttgtta
    22561 tcaatgcagc gattcttaaa atgacttcct tttttgtagg agcagaacat tttagttttt
    22621 cttcatttgg tgcagcgtta ttcgtagcta tcttgatgtc acttgtcaat gtaatcgtta
    22681 gtgaacataa cagcagaaag taaaaaaagc tgtgccgaga tctcggcgca gcttttttgt
    22741 tta
//
LOCUS       DACCKU010000044        22728 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.046, whole genome shotgun sequence.
ACCESSION   DACCKU010000044 DACCKU010000000
VERSION     DACCKU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 22728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.046"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IVN13_001584"
     CDS             23..646
                     /locus_tag="IVN13_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012079.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IVN13_001585"
     CDS             1096..2145
                     /locus_tag="IVN13_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7012080.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IVN13_001586"
     CDS             2142..3023
                     /locus_tag="IVN13_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012081.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IVN13_001587"
     CDS             3026..3796
                     /locus_tag="IVN13_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7012082.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IVN13_001588"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IVN13_001588"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP7012083.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IVN13_001589"
     CDS             5107..6270
                     /locus_tag="IVN13_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP7012084.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGGQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IVN13_001590"
     CDS             6387..6797
                     /locus_tag="IVN13_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7012085.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IVN13_001591"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IVN13_001591"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7012086.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKNEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IVN13_001592"
     CDS             9337..9654
                     /locus_tag="IVN13_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012087.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IVN13_001593"
     CDS             9814..10050
                     /locus_tag="IVN13_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7012088.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IVN13_001594"
     CDS             10047..10445
                     /locus_tag="IVN13_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP7012089.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="IVN13_001595"
     CDS             complement(11112..12056)
                     /locus_tag="IVN13_001595"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7012090.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="IVN13_001596"
     CDS             complement(12352..13296)
                     /locus_tag="IVN13_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7012091.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="IVN13_001597"
     CDS             13517..14731
                     /locus_tag="IVN13_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7012092.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="IVN13_001598"
     CDS             14753..16768
                     /locus_tag="IVN13_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP7012093.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="IVN13_001599"
     CDS             complement(16851..18224)
                     /locus_tag="IVN13_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7012094.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="IVN13_001600"
     CDS             complement(18250..18534)
                     /locus_tag="IVN13_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012095.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="IVN13_001601"
     CDS             complement(18536..20551)
                     /locus_tag="IVN13_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7012096.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="IVN13_001602"
     CDS             complement(20730..21035)
                     /locus_tag="IVN13_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP7012097.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="IVN13_001603"
     CDS             complement(21124..22155)
                     /locus_tag="IVN13_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP7012098.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..>22728
                     /locus_tag="IVN13_001604"
     CDS             22362..>22728
                     /locus_tag="IVN13_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7012099.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHED"
BASE COUNT         7393 a         4048 c         4403 g         6884 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg tgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataatactg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagca
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggg ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaatgaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctgtttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagatt
//
LOCUS       DACCKU010000045        21903 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.047, whole genome shotgun sequence.
ACCESSION   DACCKU010000045 DACCKU010000000
VERSION     DACCKU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21903
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.047"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVN13_001605"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IVN13_001605"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP7012100.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVN13_001606"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IVN13_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP7012101.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVN13_001607"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IVN13_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP7012102.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVN13_001608"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IVN13_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP7012103.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVN13_001609"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IVN13_001609"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP7012104.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVN13_001610"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IVN13_001610"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP7012105.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IVN13_001611"
     CDS             complement(8053..8523)
                     /locus_tag="IVN13_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP7012106.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IVN13_001612"
     CDS             complement(8569..8994)
                     /locus_tag="IVN13_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP7012107.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IVN13_001613"
     CDS             complement(9061..9330)
                     /locus_tag="IVN13_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP7012108.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IVN13_001614"
     CDS             complement(9341..10936)
                     /locus_tag="IVN13_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP7012109.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVN13_001615"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IVN13_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP7012110.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IVN13_001616"
     CDS             complement(14489..15049)
                     /locus_tag="IVN13_001616"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP7012111.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IVN13_001617"
     CDS             15402..16376
                     /locus_tag="IVN13_001617"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP7012112.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IVN13_001618"
     CDS             16546..17946
                     /locus_tag="IVN13_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP7012113.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IVN13_001619"
     CDS             complement(18024..18746)
                     /locus_tag="IVN13_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7012114.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IVN13_001620"
     CDS             complement(18825..19331)
                     /locus_tag="IVN13_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7012115.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IVN13_001621"
     CDS             19454..21253
                     /locus_tag="IVN13_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP7012116.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..>21903)
                     /locus_tag="IVN13_001622"
     CDS             complement(21321..>21903)
                     /locus_tag="IVN13_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012117.1"
                     /translation="TEESTTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDT
                     GNGTGSSTPAPTPTPTPEQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPS
                     GFDCSGFTRYVYMQVTGRDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAI
                     ALGGGQYIHAPQPGESVKVGSVQWFAPDFAVSM"
BASE COUNT         6060 a         4800 c         3981 g         7062 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gttttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc tcctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatgggat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttatact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggtcgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gtt
//
LOCUS       DACCKU010000046        21764 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.048, whole genome shotgun sequence.
ACCESSION   DACCKU010000046 DACCKU010000000
VERSION     DACCKU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.048"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IVN13_001623"
     CDS             complement(218..1015)
                     /locus_tag="IVN13_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP7012118.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IVN13_001624"
     CDS             complement(1012..2196)
                     /locus_tag="IVN13_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7012119.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IVN13_001625"
     CDS             complement(2221..3468)
                     /locus_tag="IVN13_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP7012120.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IVN13_001626"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IVN13_001626"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP7012121.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IVN13_001627"
     CDS             5448..5762
                     /locus_tag="IVN13_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP7012122.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IVN13_001628"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IVN13_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP7012123.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IVN13_001629"
     CDS             6447..7091
                     /locus_tag="IVN13_001629"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP7012124.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IVN13_001630"
     CDS             7106..7435
                     /locus_tag="IVN13_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012125.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IVN13_001631"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IVN13_001631"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP7012126.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IVN13_001632"
     CDS             8423..9247
                     /locus_tag="IVN13_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP7012127.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IVN13_001633"
     CDS             9240..9587
                     /locus_tag="IVN13_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP7012128.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IVN13_001634"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IVN13_001634"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP7012129.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IVN13_001635"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IVN13_001635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP7012130.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVN13_001636"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IVN13_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP7012131.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVN13_001637"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IVN13_001637"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP7012132.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IVN13_001638"
     CDS             15244..16011
                     /locus_tag="IVN13_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012133.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IVN13_001639"
     CDS             16001..18043
                     /locus_tag="IVN13_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012134.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IVN13_001640"
     CDS             18324..18473
                     /locus_tag="IVN13_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP7012135.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IVN13_001641"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IVN13_001641"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP7012136.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IVN13_001642"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IVN13_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP7012137.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IVN13_001643"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IVN13_001643"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP7012138.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..>21764
                     /locus_tag="IVN13_001644"
     CDS             21481..>21764
                     /locus_tag="IVN13_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7012139.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRP"
BASE COUNT         6773 a         3917 c         4512 g         6562 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catt
//
LOCUS       DACCKU010000047        21547 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.049, whole genome shotgun sequence.
ACCESSION   DACCKU010000047 DACCKU010000000
VERSION     DACCKU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.049"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(685..1983)
                     /gene="asnS"
                     /locus_tag="IVN13_001645"
     CDS             complement(685..1983)
                     /gene="asnS"
                     /locus_tag="IVN13_001645"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP7012140.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(2023..3213)
                     /locus_tag="IVN13_001646"
     CDS             complement(2023..3213)
                     /locus_tag="IVN13_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7012141.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNSEIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRISYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(3234..3761)
                     /locus_tag="IVN13_001647"
     CDS             complement(3234..3761)
                     /locus_tag="IVN13_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP7012142.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(3808..6597)
                     /locus_tag="IVN13_001648"
     CDS             complement(3808..6597)
                     /locus_tag="IVN13_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP7012143.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHAFFADIAHRGTGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQKLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDCGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            6855..8156
                     /locus_tag="IVN13_001649"
     CDS             6855..8156
                     /locus_tag="IVN13_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7012144.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
     gene            complement(8245..8445)
                     /locus_tag="IVN13_001650"
     CDS             complement(8245..8445)
                     /locus_tag="IVN13_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7012145.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(8897..9217)
                     /locus_tag="IVN13_001651"
     CDS             complement(8897..9217)
                     /locus_tag="IVN13_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012146.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFASTHTQKYDGHHPV
                     IYEV"
     gene            complement(9720..10838)
                     /locus_tag="IVN13_001652"
     CDS             complement(9720..10838)
                     /locus_tag="IVN13_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012147.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPEIEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            11309..12616
                     /locus_tag="IVN13_001653"
     CDS             11309..12616
                     /locus_tag="IVN13_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP7012148.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTVENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            12735..13934
                     /locus_tag="IVN13_001654"
     CDS             12735..13934
                     /locus_tag="IVN13_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012149.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTISFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(13961..15229)
                     /locus_tag="IVN13_001655"
     CDS             complement(13961..15229)
                     /locus_tag="IVN13_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP7012150.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKAEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(15392..16039)
                     /locus_tag="IVN13_001656"
     CDS             complement(15392..16039)
                     /locus_tag="IVN13_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP7012151.1"
                     /translation="MEQTIKPYQYFFIKREVEQLLNAYSSVNDPKTVQTVLALAAEKI
                     RDILEHELPEIDTFLTTVLDVKLTKAQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQEHTYIGWNDPGTQKKFMVYYEDEKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(16447..16950)
                     /locus_tag="IVN13_001657"
     CDS             complement(16447..16950)
                     /locus_tag="IVN13_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7012152.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17073..17837)
                     /locus_tag="IVN13_001658"
     CDS             complement(17073..17837)
                     /locus_tag="IVN13_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP7012153.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMMLTSK
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            17944..18219
                     /locus_tag="IVN13_001659"
     CDS             17944..18219
                     /locus_tag="IVN13_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP7012154.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            18658..19608
                     /gene="yidC"
                     /locus_tag="IVN13_001660"
     CDS             18658..19608
                     /gene="yidC"
                     /locus_tag="IVN13_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP7012155.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKKNPPKQVVTPR
                     KDVTESAKPADNKQATKQIKQQKNSTNNKGRNAGKQQKRK"
     gene            19730..20939
                     /locus_tag="IVN13_001661"
                     /pseudo
     CDS             19730..20939
                     /locus_tag="IVN13_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(21307..21528)
                     /locus_tag="IVN13_001662"
     CDS             complement(21307..21528)
                     /locus_tag="IVN13_001662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7012156.1"
                     /translation="MFKNYTINQTILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
BASE COUNT         6339 a         4460 c         3897 g         6851 t
ORIGIN      
        1 aattgaatga acgctcatca agtgtctagt tttttatgct atttttttct taacgtgccg
       61 gattcccctt gattccttcg ttgtttggtc cagagagatc gatcacaccc aagcttccgt
      121 caatcagacc actgatgacg ccatcgaaac atatatttat aggctcatct aagacttcta
      181 gtaaatgtcc ctttactaat atctcttcta gattgcttgg gatagattgg tcataacttt
      241 atctggtccg atgatttcaa gggtgaattg aacttacata tggattattg catgctgaaa
      301 atacggacct actccatctt cgaatgttac tattagtttg ttttcaggtg ctagatgctg
      361 ctttatttca acagcagaag catcatcaaa ttagatttcc atttgaatta ccttctttgt
      421 aaatgtttga ttgattgcga tttcgttata gaaccttgtg aatcaaatag caatttcttt
      481 tgctttcttc tcttgtctgc tggaaacttc ctttatcccg tatttaaaaa aaagaactgc
      541 gagagttcgc agttcttttt tttaaataaa cgttgctctc cggccaattc cttggtcaca
      601 agtcacaata atcatcaaac atgaaaaact gttttttgaa attactcctt gtgactcgga
      661 gctgaacggc agacgcacaa cctcttatgg ataaattcgg ttcaacaaac gtgggaatgg
      721 aatagcttca cgtacatggt cgataccagc aatccaagtg actgttcgtt caagacctag
      781 accaaaacca gagtgcggaa cagaaccgta tttacgtaga tcaagatacc atgaatattc
      841 tttttcatcc aaaccgtgat tacggatttg ttctagcaag aaatcatagt ccgttgcccg
      901 ttcgcttccg ccaataattt caccgtaacc ttctggtgcg atcatatctg cacaaattac
      961 cacgtcttct cttgttggat gtggtttcat gtagaatggc ttgatggctt ttggatagtt
     1021 taggataaag acaggctgtt cgaatgaatt cgcaatgaac gtttcatgag gtgaaccaaa
     1081 gtcttcgccc cattcgatgt catcaaatcc atttttcttc agtaattcga ttgcatcatc
     1141 gtaagtaata cgagggaatg gcaattttgt ataagttttt aagacttctt tgtctcgtcc
     1201 tagtacatct aatgcgtatt cgcaatgatc taaaacattt tgtactaaga atgctacata
     1261 ttgttcctgt acttctaaac tttcttcttg atgagtgaaa gccatttctg gttcgatcat
     1321 ccagaattcg atcaaatgac gtcgtgtttt tgatttttca gcacggaaag tcggaccgaa
     1381 tgaaaagact tttccgaaag ccatagcagc agcttccatg tataattggc cactttgaga
     1441 taaataagct ttttgatcaa agtaatttgt ttcgaataga tccgtagtgc cttctggtgc
     1501 agaaccagtc aagatcggcg gatcaatttt gacaaaatga tgttgattaa agaattcata
     1561 cgttgcacga atgatttcat ttctgatctg cataatcgca tgttgtcttg aagaacgcag
     1621 ccataaatga cgatgatcca tcaagaagtc tgttccgtgt tcttttggtg tgatcggata
     1681 atcgtggctc tcgcttacca cttccacatt tgtaattccg atttcataac caaattttga
     1741 acgactgtct tcacggattt ctccagttac taacacggat gtttcttggt gtagattttt
     1801 agctgtttgg aaaacttctt ccgacacttc gctttttaca actacacctt ggaaaaaggc
     1861 tgttccgtct cttaattgta aaaatgcgat ttttccgctt gatcgcttat ttgcgaccca
     1921 agcgccgatt gttactgttt ctcccacatg gtttttcgaa tcgatgattt gaatttgttc
     1981 cacgtatgac tctcctttta tattccttga atttcttcag gattaatttt gacttttact
     2041 ctcaataaat tgcttgattc tgcgtaccgc ttcttctaac gtagcgcgat cagtagcata
     2101 acttatgcgg acattttctg gtgccccaaa gccttcgcca gtcacaagcg ctacccctgt
     2161 ttcttctaaa agatcttcta cccattgtgt gacattttca tatttacaca tgcgtagcgt
     2221 ttcttttaca ttaggaaaaa gataaaaagc tccttgcggc ttttcaagag aaacacctgg
     2281 cagttctgcc acaagcggat ataacgtatt caggcgttct tcaaaggcgt tccgcatttc
     2341 ttctactgta tcttgctcac ctttcaatgc ttcaacagct gcatattgac tgactgctgt
     2401 cggattactg gttgactggg aagcaacagc aatcattcca ttgatgatct cagaattccc
     2461 caccgcataa ccaatgcgcc agcctgtcat cgcatatgtt ttagacacgc cattgatgat
     2521 gatggtctga tttttgattg cttctgaaat cgttgcaatt ggagtaaact cattgccatt
     2581 ataaactaaa cgaccataaa tatcatcagc aactatcaaa atatcatttt ttactgccca
     2641 ttcaccgatt gcctgaagtt cattcttgct ataaatcatt ccagtcggat tcgatggaga
     2701 attgatgatt acggcttttg ttttttctga acgtgcttgt tctagctgtt caacagtaac
     2761 tttccagcta ttggattctt cccctttcac aaagacagga agtccttctg ccagttttac
     2821 ttgctcaccg taactgaccc agtaaggcac aggaatgatg acttcatctt gcgggtcaag
     2881 gatcgtttgg aaaagggtat acaaagcaaa tttcgctccg tctgttacga ctgtttgtga
     2941 aggttcatag acaagtccat agtttttctt cagataatca acgattgcct gtcgaagctc
     3001 tgggattcct gctgttggtg tataatagct agctttccca ttgcggattg cttcgattgc
     3061 tgcttcttgg atgttttctg gtgtcgcaaa atccggttct cccacagtca aactcagcac
     3121 atctctgcct tttgctttta atgccttggc ctttgccgca gctgccaaag tgacagacgg
     3181 ttctaatcgt tttgttcttt ttgataactc catcccattc attcctttac cttttaaatt
     3241 cccttgattg tcttgacttc atctccagat tcaaagccaa tcaggtaata attcagtttc
     3301 ccatttgttt cttttcctac tacttcccac acaggctgat catcatacat ccctaaagca
     3361 gctttttcga tttggatttc aggatgggcc gcagcaattg tctgttttgc ttcgttttca
     3421 gtcagtccct ttgtttgatc caatactttt actttttcac cagatttagg aatgataacg
     3481 gcgatttctt ttcctttatc atttgtacct gtcagtgaaa agtatgtctt ctttcgagta
     3541 aaccaataaa aatgatcggc ttcctcaaga tttgcatatt tctttgcgat ttctactgct
     3601 tctttcttcg cctgcaccgc cggacgactg gaacgcaagt aaaaaatcga gctgatcacg
     3661 atgaccgcta ataaaaatac gatcaagcca agtaacacgc gattaatcgt tttttccttt
     3721 gtctgttttt tcttcaaggg tcgtcgctcc tttatcttca ttccttttca ttatagcaga
     3781 ttttaattat gaaaaaagac agctcctcta acttccttct tcatttttct ttaagaattc
     3841 atggatgtcc tgatagattt cctccatagc tagctctttg atcggtactt ttttcggtaa
     3901 tgcatggatg atccgtgcac catattttgc agtgactagt cttcgatcca aaataagcaa
     3961 tacgccacaa tctgtctccg tccttatcaa gcgacctaat gcttggcgta acttcaaagc
     4021 tgctttaggt acggattcct ggtaaaaagg attgatcccc tctgattcta aataagcgtt
     4081 cctagctttg accatcgggc gaagcggatt atcgaagggt aaacgtgtga ctacaacgat
     4141 ctgcaaagca tctccaggca aatctacacc ttcccaaaag ctgtcagcgc caaacaatac
     4201 gcttttttct gacaaaatga agcgtttcaa aagcttctcc cggcttccgc caaacccttg
     4261 agctagtatt tctcgtcctt tttctagcat agacagatgc attttctgat agaccttctg
     4321 caaaacatca tgagaagtga ataacacaag tattggccga tcttcttgtt cgatcagacc
     4381 tgtaagtgct gtctctaaat aatcgatata tgcttgattg ctgacttgat ggacggcagg
     4441 tgcctctttt ggcacataga tccttgcctg tttctcataa tcataaggag aggcaatcac
     4501 tttaagaggt gctgcttcaa tgccaagccg cttagagaga tagtgtcgat tagggccgat
     4561 cttcagcgtt cctccaatat aaagaatacg atcatatcga ggataccaaa cagtcgacgg
     4621 caacatagcc gcttgaaaat catggagatg aaaaatcatc tgctgcgctg tgttcccagg
     4681 aacgaaccaa tggatcaaat gatctcgcca gtcttccagc cattgatcca tcagcaagta
     4741 ctgcttgtct aattgatcaa ataagctata taaacttgct aaatcgatcc gcatctggtt
     4801 tgtccagctt tcttggataa tctcaaaagt cagacgcatt tgtgactgta gcttctggat
     4861 ctcctgataa aaaagctgca ggtgatcaat ggctgtccca gattccagtg gcagctgatc
     4921 caacatctct tttgtcatca tgacttcttc tggatactgt ccgttgggtt gcttgccgct
     4981 cactaaactc attaatcctt ctgccataca aatctgtgct tcgcatagcg cagaaagttc
     5041 ttctcgataa atcgataacc agcgtaaggc ttcatgttct ttcggtaaat agctttgtat
     5101 tcggtcaaac agctgatttg gttcttctaa ttgctggatt tgtttatgga aatagttaat
     5161 acttaatcgt ttgtcattta ctttttcagc gatttccggc aaatgatgta cttcatcgat
     5221 caacaaaaat ggactagctg gaagcaatgg ttcttttcgc tgtgtttctt gtactaaaaa
     5281 cgcatgattg acaatcaaaa aattactttg cctgattttc ttttgcaaga aacgaacaaa
     5341 gtcttgttcg taaaacgact gatttcttgc taaaaaccca gtacctctgt gcgcgatatc
     5401 tgcaaagaat gcatggttca agtttgttaa atgtaattca tccaaatcac cggtcttcgt
     5461 ttgcgtcagc caaactaaga ccgccatttg atacaaagca tattgttttt gttctggtgg
     5521 ttccacaagt gttcctttaa atcgttgcag gtcgatatag tggcgataac ttttgataat
     5581 tacaggttgt atgggctgat ctagcaattg attcagcaat ggaatgtctt tttctatgat
     5641 ctgctgctgc aaaactagcg aaacggtact gatgacagcg ggtttttcag gtgtagccaa
     5701 ataactcaat ggcagcagat aaccgattgt tttacccatt cctgtagctg cttcgatcat
     5761 caaatctttt gattcatctt ttgtgaaatg gtcgtagacc gcattcatca gtctgttctg
     5821 ttcttttcga tacatcagtg ttttaccaaa cattttttct tttgcttgtt tttttcttgg
     5881 atatgtccgc tcgccataat ggacggatgt gaatagttcc acttcttttt tctgcaaagc
     5941 tagcccatcg acgatttcta atgagggatc aagtggctct ggtttttctt tcatttcttc
     6001 aatcacatga taaatgaaac gactcgtatc catacctgtg tgtctactta atcgcgcaat
     6061 ctgctctaac gtgatgattg gcaattttct catccgttct tcgatcagca aaagaagttg
     6121 acctgtcact tcggcatcac tatctgcctg atgaggattc tcatgaataa atccaaggct
     6181 ttctgacaaa tcagctaagc gaaagcttgg ttccgtcggc aagaaaattt gtgcaagttc
     6241 tactgtatcg atgcctggta tcttcaaact aggaagtccg catcgcatga gttcatggtt
     6301 caaaaaatta taatcaaaat aaatattatg cgcgacaaat atagtgtctg ccaaaagatt
     6361 gtaaatcgtt tgggcgacat cttcgaaata aggcgctttt tggacgcgtt gattcgtgat
     6421 ccctgttaaa tgctggattt gttttgaaat cttccgccct ggatgaatat cgatcgaaaa
     6481 acgtgaagtg atccgacctg attcaatcat gacgcagcca aattggatga tccgatcttc
     6541 ttttggattc gtcccagtcg tttcgatatc aacgactgca taagttcttg gtttcaaaat
     6601 atcttcctct tctcctgcct ctaatgttct tttgaaatta tactacaaag tgcagaaaaa
     6661 tactctcatc gctttttaaa gaaaatagac aaaaacgccc agacacgcaa atgtctgggc
     6721 gtttattttg gctctataat ttctaggact gatagaaaat cattctatcg gtcccttttg
     6781 agcatgaaaa aacaaaacat acagaagctc cgttagaatt gaatcaccac aacacaatca
     6841 aaggagcgtt ctgtatgcct gatcttcatc atatcaaact actacttgga atcaaagata
     6901 aaaacatttt catcacaaat gtggaatcga agagcattaa gaatatcaag agtcttgttg
     6961 tttctgcgac gttaagtaaa gaaattcgtc gctgtccttt gtgtaaacag atgaatcatg
     7021 aaggcatgat cgttaagaac ggaaagaaaa aatcgctcat tcaacttaat aaatgtgcga
     7081 accaactgac ttatcttgct ttagcgaaac aacgctacca ctgtcgtggc tgccacacct
     7141 attttacggc gaatacctac attgtcgata gaaactgctt tatcgctaaa caagtgcgct
     7201 ataaaatttt agaagagcta actgaaaaac aagcaatgac gacaattgct aagcattgcg
     7261 gcgtttcttg gtcgaccgtt tctcggacac tcgcttctct gatccccatg acaaaagtaa
     7321 aaagaaattg gttaccgcgt tgtttattaa tggatgaatt tcgttcactg aaaaatcaag
     7381 tcggtcccta ctccttttct tgtatggatg gcgatactgg aaaattactg gatattcttc
     7441 cttcacggaa gaaaaaggct ctcgtttctt attttatgca atttgaacga cgcgcacgat
     7501 tgaaggtaaa ggtcatcgtg acggacatga atgtttctta tgcctctctg ataaaggaat
     7561 gttttccaaa ggcaaaactc gttgtggatc gttttcacat tgtcaaacat ctcattcgta
     7621 aatttgagga tattcgcgta cgaatcatga aaagctttga tcgaaacgat ccaacacaag
     7681 ccaaatatta tcgacaatta aaggcgttgt ctcggcttct gatcaaacga caggacaagc
     7741 ttgtttatga caaatggacc aaatggcgca attttggttg ggcgtatctc accgaaagtg
     7801 aagtggtgga tcgtctgctt tcgatctctg acgaattacg aatcgcctat ttttactatc
     7861 aagaaattct tcaagcgttt cacgacaaag aggcggacac tttctttaaa cttgtaagaa
     7921 cgatgcccaa ttccgttcca aaagaactgc attctataaa aaaagctttt ataaactatg
     7981 aatccggcat tcgtttagcc ctcgaattgc cttattcaaa tgccaaaata gaaaatctgc
     8041 atacccacat caaagcattg aaacgagtcg cttatggctt tcgaagcttt cggaaaatga
     8101 agaccagaat tttcttacta aataatctga ttacctatga atcaaaaaac atctaaagta
     8161 gaaaaggaat tctaccttag atgttcttat ctcactctat cagtcctatt tgacaaagag
     8221 cctttatttt ttagtatagg ttaattaagc tttagtaacg tttgttgctt gaggtccgcg
     8281 ttggccttct tcaacatcga aagttactgc ttggccttct tctaaagttt tgaatccgtc
     8341 gccttggata gctgagaaat gaacgaatac atcgtttccg ttttctgcag tgatgaatcc
     8401 aaatcctttt tctgagttaa accattttac tgtacctgtt tccataataa aaatcctcct
     8461 tgtgtatcaa cacatatttt gcaattactt gaatggtgta tatctggaag atgtttattt
     8521 gaaacaagct accacagatt accgaacaaa aactactcct atagtatatc ataccgttta
     8581 caaaaaagct agagtttttc gacttttttt attaaaaaaa gttttctcag cgattacata
     8641 aagttaaaaa ccgaactttt tttaaaataa acgacatatt gttcagttgg tgtttttttg
     8701 ctctgatgtt cttctttaaa ctaaaaaaat tattctttct gactacttat cggttctgtt
     8761 tcaacggtcg aggctgggga agattcttta gtaggtctga tgttctcatg gtatttctga
     8821 tagcctatga cagtgaccag aagaacaagg caaaccaata cgatcaacga gattagtttt
     8881 tcttttttgc tcatatctat acctcataaa taacaggatg atgtccatca tatttctgag
     8941 tatgagtaga agcaaaagct gcagctgtag aatgtaagaa atgccagcaa ctttcataat
     9001 cacaggtcgg acagtaccaa aagtaaccaa atcttggctg caaagaccgt tcttctgttt
     9061 caggaatcgc agaatcaaga tctgttgtct gtacataaac atttttgtac agacgtgtgc
     9121 ctgtaatagg agaccgctgt gtttcttcag caaaaactgg ctgacttgtt aaacttaggg
     9181 atagaaacag caaacaccat aacagtcttt ttttcataaa gacctcctta tcgataagtt
     9241 tttcccaatt aaaatcatat ctcaagaacg cccgttattc acattttgtt cataaatgat
     9301 gttttttgta tcataatcgt caaaaagagt tcgtgaccat gtcacaacct cttttttaaa
     9361 cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa tttgagaaat
     9421 aatttttgta aatctcttct tgtttcttga agctgactac ttctatcaca acctcttcct
     9481 taaacggtgt tcaaaagctt gcatctgcgg taataagccc accaaacaaa aaatatgaag
     9541 agctattttt gcttgctgtt cttattactt gatgcagaac agctttttca caacctctca
     9601 tacacggtgt ttaatcagct gaccttcggt attagctcaa aaaacggcaa aatatgtgaa
     9661 actatttttc cgtttgtttc tctaacactc aggtcaaaac actgatttca caacctcttt
     9721 taataacgaa gaatcgctgt cagactcttt tcatctggcg catctttttg atccaaaata
     9781 aatactttcc cgccattaat gatcacatca tctgctaatg cattcaacac ttgacgccga
     9841 tcatattcta tttcaggatc ttcaccgaaa ccatcagcta agtttgacgt agcgataaat
     9901 agatgcgaca cttttccttc tttcgcagct ggaacgatat caactaactg atcgatgaat
     9961 ttcttatcta agagtttatt gtattctttt gattcttgag ctgcgagttc acttgacagc
    10021 ttcttcgccc cttcttcgat atctctaaag gatagctgcg caggagaagc agaaatcgca
    10081 acgtctgcac aatagtaagg atttttggcg atttttttga acatcgtctg attttctggt
    10141 agagcgaata aataaatcgg ccattgttct ggattttcga aagaatcctt caagtacgta
    10201 tcaacagctt ggtaatagtt gacccaatcg atctctactt cttcatcttt tggatttacg
    10261 ccatggaaag ttactccatc tcgggctccg cctcctccat tagaagagaa gttcaaaccg
    10321 ccacctgtca gttcagatcc caatgcagta acgacatcaa ccggcgcccc ttctggtaga
    10381 gatacttctt ttacttgttt gttgctgacc tcatacaatt tcatggagtc gcggttcaat
    10441 cccaataaat aataagaata gttgaactgc ccatttttga taatagccag taaataagga
    10501 agatcactta catagtattg atcgtctacc gggatactca aacgatggat ataggtttgg
    10561 tcagcagtta taatgaccgc tacactcgta gttgcactac gccaaaacga aggatctcct
    10621 aacaaatttt caattttttc ttggaacggg gaccagtcat gttcggaata ttttttctca
    10681 aaacgtttct ttgcttcttt cgcaaaattt ttgaatttga tttgatcttt ttccacatct
    10741 tgatgggcga catgcgtatt cagcatgatc gtgacaaatg gtcctttagc gccttctgaa
    10801 gtcaattgtg acaataattc tttatccttt tttgccataa acgtacccct cctaaaaaaa
    10861 tcattccttt gtttctatct aaagtctacc acaacactat ttttccatac aataaaacgt
    10921 atctaaaaaa ataaaacggt ttctacggtt ttaaatttag ctattttttt aagttatctt
    10981 cataatttcc tcaattatat agccccagtc ttcaactatt cttcatgaaa cgctgataaa
    11041 ctaagaaaaa gaataaaaga ggtggatctt atgcatttga ttatgatttt cagtttatta
    11101 agtattatca gtttatttgc tttcagcgct tcccgcttat tagatgcgga aacacagcct
    11161 attcctgtaa aagtacgtaa aaacgaccgc taaccttcca taacgtgcgt tcttatccta
    11221 aagttttttc agaaacattg aaactctctc ccctctctga taaaatttta atttgtagcg
    11281 aaacaaatta attacaagga gatcggatat gtccataaaa aaaacacgtc tagtcctatt
    11341 attggctagt ctcattcttt gccttgcttc ttttctacca gctgttgctg tgcaagctga
    11401 agatacattc aaagtaaatg caaaagcagc ttttgctgta gatgcgcaaa gcggtaagat
    11461 tctttatgat caagatggag aaaaaaccat ggggatcgct tcaatcacaa aaatcatcgg
    11521 tctttacatc gtccttgatc aagtaaaaga aggaaaacta tcatgggatg ataaagtttc
    11581 gatttcagat tatgcagaaa atttgagtat caccccagat ttatcgaatg ttcctttgca
    11641 taaagaaaac acctacactg tcaaagaatt attcgactct gcaatcatcc agtcagccaa
    11701 tgcttcaatg gtggccttgg cagaaaaaat ctctggcagc gaagctaaat tcactgaacg
    11761 aatgaaagaa caattgaaag attggggaat caaagatgca acaatcgtta atgcatcagg
    11821 attgaacaac tcttatcttg gcgaaaaccg accagaaggc actgtagaaa acgacgaaaa
    11881 tcaaatgtct gctcaagatg tggccatcgt tgcccgccat ttgatcttgg atttcccaga
    11941 aatactggat gtatcaagta ctacgaccca aatgtttggc gaaaatacgc aatcaccagt
    12001 ggaaatggtc aactggaact ggatgctccc aggattcgtt aattacaagg aaggcgtgga
    12061 cggattaaaa actggtacga ctgagcttgc aggtgcttgt ttcgttggta cgatcactaa
    12121 aaacgatcaa cggatcatca ctgtcgtatt gaatgcggac ggccatgcgg aaaacccgag
    12181 tgcccgcttc aatgaaacag accgtttgat ggactactgc tatgacaatt ggtcagaaaa
    12241 agagctcgga aaagctaacg caagtattcc tagcctaaaa accattgatg taaaagacgg
    12301 aaaagaatca acagttcctg ttgtgcttaa aagccctgta aaagtatggg tacgaaacga
    12361 tatggatact ggcaaattaa cgatcacgcc aacacttgac aagaagcaag tcactgacaa
    12421 cgagatccaa gcacctatgg ataaaggtac aaaaatcggt accgctacga tcaccttggc
    12481 agacgataaa ttaggttatt tagaagataa caatgcgcca tcgacagaga tcattacgga
    12541 taaaacagtg gaaaaagcga atatattcgt gattggctgg cgacgcgtcg cagaattttt
    12601 cggcaacttg ttttaattga acaataagaa aaacatcttt ccatcggaaa gatgtttttt
    12661 tgctttttct agtttaaata acagcttttt tcatctattt tcgttatact gtataagaaa
    12721 aaggagttga atttatgaat ctaaccagag atctgatctt tttagttctc tatcttattg
    12781 gggtattctc tatcgtttat tggcgaaaac agaagaagga tccttccttg ttcttttacg
    12841 gcagtacatg gacatttacg gtaatcttaa cttattttgt cgtgttggaa attttttatt
    12901 cgacctttta ttttttgatt ccattgttgt tctttagtat ctttgctttt tcttatttca
    12961 cagaaaaacg aaggctgctg aatggtcttt tattcaatgt tttcctcatc agtttcggca
    13021 tctacttgtt tgtcttgctg tatgaaacgc aaaatatttt ccttggcgga ctgatcgcct
    13081 tgatcacgat tccattgcta cttgtccttt tatttggtat ttatggattg attgtttttc
    13141 ttttttggaa cggggtcaca gttttacgta gagaatcgca ttcattagct aatcttttaa
    13201 cgttgatact agccattttt cttacactgt ttcttgtttt tgactttttc ttgttaaaat
    13261 atttgccgca atggataaat gctttgtttt tctgcgttcc cctgatcttg atctacctat
    13321 ttatcgtatt ctacaacttt ttgactatct cattcttgta tcagttcaac cggcctcgat
    13381 acaatcaaga cttcatcgtt gtgttaggcg ctgggttgat caatggagaa acagtttctc
    13441 ctcttctagc caagcgaatc aataaggcaa ttgcttttta tcgggctcaa agccgtgcta
    13501 cattaaatcc gcccatctta ttgatgtctg gcgggcaagg agcagacgaa aaagtaccag
    13561 aggccattgc gatgaaacaa tatgccatgg aacaaggaat ccctgaaaga gacattcttg
    13621 tcgaaaccaa ttcgacaacc actttggaga acatgctcta ttcaaaggaa atcatggatc
    13681 agcaaatgaa agggccatac cgcgcgatct ttagttccaa caattaccac atttttagag
    13741 ccggtcttta tgcgcggcaa gccaaattga aagcaaatgg tattggtgca aaaacagctt
    13801 tctactactt gcctaatgcg tttttaagag aatacatcgc catcttgatg atgaacaaaa
    13861 aaagacatat gatcatttgc ggattgcttt tcgcaggctc cgcattctta tcgctgatta
    13921 ctttactttt ctaaaaaaac aaggtgtgac aaaacatatg tcacaccttg tttttttctt
    13981 tcattctctt aactattggc aaaaagagta tccctttttt gaccgctact tgctgagcag
    14041 aataaatacc gctgttgcct gaacacataa agctgatcaa gcagatcata aagaagtata
    14101 ctgccgcttg tgaaccgaac aattcgatcc ccatcacaaa gcaagcaatc ggtgtatttg
    14161 tcgcaccaga aaatactccg ataaagccca atcctgcaag aaaaggaaca gaaacatgga
    14221 gcaaaggcgc tagtgctgca ccaagcgttg caccaatttc aaacaatggt gtcacttctc
    14281 ccccttgata tcccgcgcct aaagacaaaa ctgtgaagaa taacttccca ataaaatcat
    14341 agacatgtgc ctgtccatta aatgattctt gaagtaacgg aagactcaat ccgagataac
    14401 gttgtgtttg caaaatcaat gctgctgcca caactacagc tccacctatc gtatttcgcc
    14461 aaacaggatg aggaatccat tgtgcatatc gcgcttttaa aaagacgatc gaacggctga
    14521 atacccaacc aatcaaacca aaagcaatcc cagcaacaaa aactttgaag aacagcaaca
    14581 tactccattc aggaatcaca cccatttggt aatgtagatg agaaacacca tagctttctg
    14641 tcatgaaatt tgcaaacagt gcagcaaaga agctagggaa gatcgcttct gttcggactt
    14701 tcccgattgc caacacttct aatccaaata atgttccagc taaaggtgtg ccaaatacag
    14761 aactaaaacc cgcactgatt ccactgataa tcaagatttc ccgttctgct ttatccaatc
    14821 ggaacaaacg tgctgtatta tctgctaacg ctccccccat ttggactgct gttccttcac
    14881 ggccaacaga tcccccaaat aaatgggtag ttatggttcc aaacagtgta agtggaataa
    14941 gccgcaacgg gattttttct tctccaccat ttccttgatc gatcactaag ttgtttccac
    15001 gactagaaag accgccgtga tacgcataga gataagcaaa aagcgcacca ctaactggta
    15061 gtagataaag gagccaagga tgtgatagtc ggatatctgt cacaactgtc aggctattta
    15121 agaaaaaagc agataagctc cccatgaata taccgagaat cgctgtgatc accagccact
    15181 tcagcatata cactaaaatg cgcactggac tcggtaattg tttttccata aaaaattcct
    15241 cctcaataaa aaaacgccta ctaaagaaag gggctgtccc cagactttag taggcgtcat
    15301 tagctatctt tcgttagcat ttaaaggcga acaccatcac ctattcactt ccattaaaag
    15361 atagcatatt tttctgtaaa aaacaataaa attatcgttc tttctttaca cgttcagcaa
    15421 attcatagaa aggagccaat ttcgttaact gttgattaca ttggtcacta tcatgacaaa
    15481 tataatttcc ctttttcgta tacgtgccat cagaacctgc tttcgttgta gaaagaaaaa
    15541 gcgaaacatt cgatgttttc tgacagattg cacagattcc cttatttatc gttggtgata
    15601 aagtaccgct aatccctttt aatttttcat cttcgtaata gaccatgaat tttttctgcg
    15661 tacctggatc attccagccg atgtacgtat gttcttgcaa gtcgatttca ttccaagctg
    15721 gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg ctaggctgca
    15781 taaacggctg gacgatttgc ttcagctcct ccaatgctct ttctgcttgc gcttttgtca
    15841 gttttacgtc taaaactgtc gttaaaaatg tatcgatttc tggcagttcg tgttctaata
    15901 tgtctcgaat cttttcagct gctagtgcca gaactgtttg taccgttttt ggatcattga
    15961 cagatgagta cgcatttaat aactgttcta cttctctttt tataaaaaaa tattggtatg
    16021 gttttattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
    16081 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact cttttaaata
    16141 agatacgttt tgcatcttat catcaccccc tttaatgctt attcttctat gtacatgatt
    16201 cattcgataa ataaaagtaa accgtatctt gaacagatag tcaactgcaa acagaaaaaa
    16261 ggactgtgac aaaagtcgtg tcaaagtccc ttgtttcgaa tgaatggtgt tcaaaagctt
    16321 gcatctgcgg taataagccg aaacttcaca aaaatttgag aagtaatttt cgtaaattcc
    16381 ttcttatttc ttgaagctga ccacttctgt cacaacctca atatagtcta ttattggttt
    16441 tacctattaa atcgaggatt ctttcccaaa gtaacgttgc catctcttac gcatacttgc
    16501 agacatgggc tgagccgctt ctttgactgc gcagtatttg tccacgaatg tcacgatata
    16561 tccttctttg tacttaggcg gtgcaatcgt tgcaccccac atgtgcttca aaatgatatc
    16621 tcgctcaaga tccgacaaat cggtgatttt ttctgcattc tttacagcaa ttcttggatg
    16681 gatatatgca tgtgtcccct cgtcaaattt cgtcgtacgc caatcatagt agaacagatc
    16741 atgcaaaagt ccagcacgcg ctgttgcttt agcattgcca ccccattttt ttgcgagcag
    16801 ataactatag taagaaacac tgatggaatg ctccaaacga gtggaatgaa catgctgtgt
    16861 gtaattcgct aattttttca ctgcttctgt ttctaaaaga tcttgtacat aagacatata
    16921 ttcttcgtct tcacgccatt tttctgtcat aattatcgat ctgccacctt tttgaatttc
    16981 attttcttct gaaattgatc taagtatacc accagtgttc ggattattct agtcgctcta
    17041 gataaaatag ctaaagctta atagttcctt aatcataaag aaaaatgata aagaacgatc
    17101 cctgctgcga ctccgacatt caatgattcc gcatttcctt tcattgggat atatacgttc
    17161 tgatcagtca aagacaagat ttcctgactt actccctgac cttcgtttcc catgattata
    17221 gcgaaatcct ctgttttttc tactttgtct aatgcatgtg cttcttcatt taattctgta
    17281 ccgtacaccg taaacccttt gcctttcatt acagggatca cttctgccaa atctttttgt
    17341 aacacaggca aatgataatt gcttccctgc atgcttcgca gcacctttgt actataaata
    17401 tctgccgttc ctttacctaa taaaacacca gccataccaa acgcatctgc cgtacggatc
    17461 atcgttccca catttccagg atcctgtaca ttatctagta aaagccagcc tcctgaaaaa
    17521 tctggcgtat ctttagatgt taacatcatc actgctaaga tcccttgtgg tgtaggtaga
    17581 tctgacatag cggtcatgac ctcatctgaa actagaaaag tagaaatctc tttgtttaca
    17641 gccagccagt ctccccattc tttcatacct ctctgtgtca caaaaagttc ttttatttct
    17701 gcatcagacg acgcagcttc ttcgatcaga tggaaccctt ccaaaagata ttctgccttt
    17761 tcttctctgt atttcttttt atgtagtttt tttaattctt tgattcttgg attttttgct
    17821 gattggatct ctttcacatt cctcacctca cctgattata tcatggaata ggtgcttgag
    17881 cttgtttcat cctaaaaaaa tcggtatcat tgatttataa aacaggaaag aaaggtgacg
    17941 aacatgcgca aagttaaaat gaatgtccaa ggacgtgtcc agggcgtggg atttcgctat
    18001 atgaccaaga tggttgctga tcagttaggc gtgaccggaa cggtaaaaaa cgaagacgac
    18061 ggctcagtca caatcgaagc aatcggaaat gacgacatca tccaaaaatt tatcgaagaa
    18121 gtcaagaagt ctccgagccc aagtggacga gtgcagtatg tggatgtcca agaagatccg
    18181 atgattgagg aacgaaagaa atttgatgtg gtgggataag gttgtgaaaa agctggtctg
    18241 catcaagcat taaggactgg ttgtgaaaaa tagctctcca tatttttcaa caggctggac
    18301 ttaatgccgt agatgcaagc ttttgaacac cgtcgataag gttgtttttg aggcgctttg
    18361 cctcgagtat taaaaacagg tagtaaaaaa gctgatctat aacttgcaat aaatttgctt
    18421 aaccctcatt ttccagacag aacaaaaaag tttttagttt tttaaataag aaagaaacac
    18481 ttggaatcac tcttttcttt ccaagtattt ctttcttgat tttttgtctt ttttcaaaat
    18541 ttttcgctac gaaaaagaaa gaatatttat atgaaatttc tatgaagttg attgaattcc
    18601 atgcgctttt ttagtatagt taagtttgtg taaaaattat agaagaggaa gtattctatg
    18661 aataaatgga aaaactggtt attgggatcc ggacttgtca gtatcctgct ctttttatct
    18721 ggctgcgtgc ggatggattc aaacggaaac cctgacactt caggaatcgt ttatcgagta
    18781 ctcgtccatc ctatgggcca agcgattaca tatctagttg agaatttcaa ttggtcttac
    18841 ggctgggcag ttattgcaat gactgtgatc gttcgtatca tcatcttgcc actaggtatc
    18901 agtcaatcga aaaaaacaat gatccaatct gaaaaaatgc aagcgttaaa accacaagtt
    18961 gaagcagcac aacaaaagct aaaagctgca acaacacgtg aagaacaaat ggcagcacaa
    19021 gcagaaatgc agcaagtata tcgcgaaaac ggattgagta tgaccggcgg aatcggctgt
    19081 ctgcctctat tgatccaaat gccgatcttc tctgccttgt attttacggc acgctacaca
    19141 gaaggtatcc gggaatcatc tttttacggc atcgatctag gctcaccaag tatggtgctt
    19201 gtggccatcg caggggtcgc ttatctgcta caaggatata tttcaacaat cggtattcct
    19261 gaagaacaga aaaagacgat gcgtacgatg ctgatcgtct ctcctgctat gatcgtcttc
    19321 atgtcgatca gtgcgccagc cggtgttaca ctttactggg tagtcggtgg tgtgttcagc
    19381 tgtctgcaaa cattcatcac aaacgttatg atgaaaccac gtttgaaagc acaagtggca
    19441 gaagagttga aaaaaaaccc gccaaaacaa gtcgtcactc caagaaaaga cgtgacagaa
    19501 tcagcaaagc cagctgacaa caaacaagca acaaaacaaa tcaaacaaca aaaaaacagt
    19561 acaaacaaca aaggccgcaa cgcaggaaag cagcagaagc ggaaataaaa atatatgaca
    19621 aaaaactgta gacaaataaa aaaatttctt tgttctacag tttttttgtt acaattaaag
    19681 aaaacgttcg cttttcgagg aggaatgtat aatgatgtcc aaataatcaa tgtatagtcg
    19741 aatggaagaa gacaaaattt cgctagatgc acttgttcct aaaaatcatt tagtccgtaa
    19801 aattgacaaa ttattgtcat ttgattttat ttatctcatt gttgaatcaa cttactcgac
    19861 cattggtcgc cctagcgttg atcctgtcgt cttagtaaaa ttagtattca ttcaatattt
    19921 atttggaatt tgttcgatgc gtcagaccat taaataagtt gaaacaaacc tagcttatcg
    19981 ttggtttctt gggcttagtt taattgataa agttcctcat ttttcaactt ttggtaaaaa
    20041 ctatgcacgg cgtttcagag aaaccgattt gtttgaacag atatttatca ttattctcga
    20101 ataagcagtt gatgctagat ttattcatga agaaattttg tatatggatt caactcatat
    20161 taaagcgaat gcgaacaaac gtatttttac aagagatatg gcacataaag aagccatttt
    20221 ataccaaaaa caattggaag aagaaatcaa tgaggcaaga attggcgaag gcaaacgccc
    20281 ttttggttca atcattcgtc aggaggtggt cgaaaggaaa tttagcatcg cagatccaga
    20341 aagtgtttat tatgtaaaaa cagaacgaga aaaacagttt gcttattctg ctcataccgt
    20401 tggtgatgaa aatggttttg tattagatgt catgattacg gctggaaatc tacatgacag
    20461 ccgaatgctc gtgccacaaa tcgaacgcgt taaatcacgt tatggtgtag ctgctaatgc
    20521 tgcgtacaaa acaccttgga acgccaagta tttaatcgag cgaaaactac gacccatttt
    20581 cccttatatc agtttcgcac aactactcga gatggctata aaaaatatgt atctaaatct
    20641 tttatttgtg aaagttatcc attattgaaa aattgtacag aaagccaaaa acaccaaaaa
    20701 gaaatacatc gacatatttg ggaagactac ttagatgaag cagaacattt acgccatacg
    20761 gtgtacaatc gtgaaaaata taaaaaaaga aaagaaacga ttgaacgcat ttttgcagac
    20821 gcaaaggaaa aacatggaat gcgctacacc aagtatcgag gcttggaaaa aatcagatgc
    20881 atacgatgct tgtttttgtc gcaatgaatc tgaaaaaatt aaccacttgg cagtgggagc
    20941 atatgtcgtt atatctttta ttatacaaaa atagacaaaa gtatcagtct acactcttag
    21001 taaagaggaa actgatgctt ttgtctacag tcttagagag tgggacaata gtcttttctc
    21061 caaaagaaaa caagtccgac tttgatagtt aatttcaaaa tcggacttgt tttttcttct
    21121 ttacgtcttt tcctctccat gtatttccct aattttctta atttaacgcc atgagtgcca
    21181 agccaatgtc aatcttggct ccttgtttcc ttcataagtg gaaatccgtt tccgatagat
    21241 cgaaccttct ttttccttta aaagtttttt tacattctgc tttgaaataa cgccatgtat
    21301 tattcactaa aatttgtcgg tttcgctcac tttttgcttt cgtacattga ctacgaacag
    21361 gtcagcaccg accactttca cattcgtaaa ttttgaagac acgtataaat ccacttttat
    21421 cttttcttcg actaaagcgt tgaccgcaaa agcgacatct gtttcagaaa tatattgttc
    21481 catatctaat ggtaaaattg tttggttgat ggtataattt ttaaacataa agcactcgct
    21541 tcctttc
//
LOCUS       DACCKU010000048        21394 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.050, whole genome shotgun sequence.
ACCESSION   DACCKU010000048 DACCKU010000000
VERSION     DACCKU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 21394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.050"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            185..1183
                     /locus_tag="IVN13_001663"
     CDS             185..1183
                     /locus_tag="IVN13_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7012157.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="IVN13_001664"
     CDS             1295..1804
                     /locus_tag="IVN13_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP7012158.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="IVN13_001665"
     CDS             complement(1834..2493)
                     /locus_tag="IVN13_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP7012159.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="IVN13_001666"
     CDS             complement(2495..3259)
                     /locus_tag="IVN13_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP7012160.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="IVN13_001667"
     CDS             complement(3267..3944)
                     /locus_tag="IVN13_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP7012161.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="IVN13_001668"
     CDS             complement(3981..5366)
                     /locus_tag="IVN13_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP7012162.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5335..5589)
                     /locus_tag="IVN13_001669"
     CDS             complement(5335..5589)
                     /locus_tag="IVN13_001669"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012163.1"
                     /translation="MSDYNETTTDFRVCFFYFYSTVKMNVPDFLARLIYRILYLYNLL
                     QGRKNSEIELVSMEEKAAYGMIKIKRGERVHGKNSDFTYE"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="IVN13_001670"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IVN13_001670"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVN13_001671"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="IVN13_001671"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP7012164.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="IVN13_001672"
     CDS             6305..7162
                     /locus_tag="IVN13_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP7012165.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="IVN13_001673"
     CDS             complement(7243..7578)
                     /locus_tag="IVN13_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012166.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="IVN13_001674"
     CDS             complement(7645..9015)
                     /locus_tag="IVN13_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP7012167.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="IVN13_001675"
     CDS             complement(9093..9629)
                     /locus_tag="IVN13_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7012168.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="IVN13_001676"
     CDS             9938..11419
                     /locus_tag="IVN13_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7012169.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTTKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHKLYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="IVN13_001677"
     CDS             complement(11522..12229)
                     /locus_tag="IVN13_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP7012170.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="IVN13_001678"
     CDS             complement(12308..12646)
                     /locus_tag="IVN13_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP7012171.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="IVN13_001679"
     CDS             complement(12757..13719)
                     /locus_tag="IVN13_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP7012172.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGL
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="IVN13_001680"
     CDS             complement(13734..15065)
                     /locus_tag="IVN13_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7012173.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="IVN13_001681"
     CDS             complement(15073..15753)
                     /locus_tag="IVN13_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP7012174.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="IVN13_001682"
     CDS             complement(15972..16451)
                     /locus_tag="IVN13_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP7012175.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="IVN13_001683"
     CDS             complement(16578..18257)
                     /locus_tag="IVN13_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7012176.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="IVN13_001684"
     CDS             18805..20307
                     /locus_tag="IVN13_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012177.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="IVN13_001685"
     CDS             20320..20982
                     /locus_tag="IVN13_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012178.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="IVN13_001686"
     CDS             complement(21083..21286)
                     /locus_tag="IVN13_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP7012179.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
BASE COUNT         6354 a         4435 c         3635 g         6970 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaac caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca taaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag agccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgtt
//
LOCUS       DACCKU010000049        20985 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.051, whole genome shotgun sequence.
ACCESSION   DACCKU010000049 DACCKU010000000
VERSION     DACCKU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.051"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(344..1465)
                     /locus_tag="IVN13_001687"
     CDS             complement(344..1465)
                     /locus_tag="IVN13_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP7012180.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWKGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            1591..2532
                     /locus_tag="IVN13_001688"
     CDS             1591..2532
                     /locus_tag="IVN13_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7012181.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(2682..3578)
                     /locus_tag="IVN13_001689"
     CDS             complement(2682..3578)
                     /locus_tag="IVN13_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP7012182.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            3847..5187
                     /locus_tag="IVN13_001690"
     CDS             3847..5187
                     /locus_tag="IVN13_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7012183.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            5405..5848
                     /locus_tag="IVN13_001691"
     CDS             5405..5848
                     /locus_tag="IVN13_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP7012184.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(6352..7770)
                     /gene="nhaC"
                     /locus_tag="IVN13_001692"
     CDS             complement(6352..7770)
                     /gene="nhaC"
                     /locus_tag="IVN13_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP7012185.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(8173..9396)
                     /locus_tag="IVN13_001693"
     CDS             complement(8173..9396)
                     /locus_tag="IVN13_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP7012186.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(9427..10266)
                     /locus_tag="IVN13_001694"
     CDS             complement(9427..10266)
                     /locus_tag="IVN13_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7012187.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(10283..10963)
                     /locus_tag="IVN13_001695"
     CDS             complement(10283..10963)
                     /locus_tag="IVN13_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012188.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(10965..12038)
                     /locus_tag="IVN13_001696"
     CDS             complement(10965..12038)
                     /locus_tag="IVN13_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7012189.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(12685..13356)
                     /locus_tag="IVN13_001697"
     CDS             complement(12685..13356)
                     /locus_tag="IVN13_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7012190.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(13443..16121)
                     /gene="mgtA"
                     /locus_tag="IVN13_001698"
     CDS             complement(13443..16121)
                     /gene="mgtA"
                     /locus_tag="IVN13_001698"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP7012191.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            16780..17217
                     /gene="ndk"
                     /locus_tag="IVN13_001699"
     CDS             16780..17217
                     /gene="ndk"
                     /locus_tag="IVN13_001699"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP7012192.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(17239..18105)
                     /locus_tag="IVN13_001700"
     CDS             complement(17239..18105)
                     /locus_tag="IVN13_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7012193.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(18265..20358)
                     /locus_tag="IVN13_001701"
     CDS             complement(18265..20358)
                     /locus_tag="IVN13_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP7012194.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         6115 a         4332 c         3704 g         6834 t
ORIGIN      
        1 tcaaaattta gatttgcttg ttcaaataat aatgtgcctt ggctgtcgta gccgaatgtc
       61 agatttttta tttcgatttt agacattttc catcacctcg taaaaaaaga ccagtcctat
      121 tttcagaact ggctaaagag gatgcaaggg ttggatacac acaaaaagac ccatacatcg
      181 aaaaaaccaa gccaatcccg aaaatctgca cactgcttcc ttgtctccac aaggtacagt
      241 ctgaatgcag ctttcatcaa tcgacttaat ttttcaatgc acggatccct cactttgtcc
      301 tatatttgaa attacgatag cataaagaaa atgattttgt caactacttc gggaggatgg
      361 tgaaaaatct gctaagtgtc tgtcctaaga tttttgtttt ttctaatgtt tgcctgtgtt
      421 ccattgcttc ttctaaacct attggaccca aatgaatact taagacaatt gggagaaaat
      481 cagaaagtcc ttctagctct tggtctctac ttccgcaaat agcgattatt ggttttcgat
      541 atttttttgc aagcactgct acaccgtatg gaacttttcc gtggatcgtt tgctgatcga
      601 tccgcccttc tcctgtaatg atgacatctg cttgttcgat ctttttttcc aatccagtga
      661 tttggctgac taaatcgaaa ccagaaacca tcttcgctcc caacaaaaaa agggctccac
      721 caataccgcc tgctgctcca cttcctggaa tatcatctaa atccacctcc cagaaatctt
      781 ttatttgctg agcaatcttt cctgcttgtt tatctaagaa agctagcgtc gtatctattc
      841 ctcctttttg tcttccaaat tgccaagcag ctccttgttg acctgtatat ggattggtaa
      901 catctgcagc gataatcaat tttactttag aaacaagaac gtttcttcga tcaaaatgaa
      961 gttttttagt agataataaa ggatttcctt ctgtgtaccc ttctaaagaa gcacccaagg
     1021 attggagtaa tcctagacca ccatcgctac acccgcttcc tcctaaagtg acaataattt
     1081 gttctacttc ttgaagcagt gcatctttaa taagttctcc taaggcataa ctggaagcaa
     1141 gtcggatagt ttgatccgtt ggtttatcaa gtagatctaa ccccaatgct tgtgcagatt
     1201 cgatgactgc ggtgtttttt cctttccaag ttgtcaataa atagctgatt tttcgctgac
     1261 ggaataagag atccatccca tcaatggtac gccatgttcc cctgagcgcg taatagattg
     1321 cttctgcagt attttctccg ccatctgcaa tcggcacatt gatttttctt cctttacgat
     1381 cttcccagcc atttagaact gcctgattta gttcaaatga agaagcactt cctttaaatg
     1441 aatcaattgc cgccaaaata tccatctgta tccctcactt tcttcttaag taagacactt
     1501 tttttcattt ttttcaaaca aaacaatgct attttcttcc atttttctat atactaaaag
     1561 ggaactcatt gaataaagga gagaaaaaac atgaaaatcg caatagctgg ggccggagca
     1621 atgggcagcc gaatcggatt gatgctacat caaagcggaa atgaggtcct cttgatcgat
     1681 cgttggcctg ctcacattga agccattcgt acaaatgggt tgattgctga ctttaacggt
     1741 aaagaagtgg tcgcaaaact gccaatctat tcaccagaag aaatcatcga aagcaacgaa
     1801 cacgtggact tgatcgtagc attaacaaaa gccaatcaat tagatgatat gttttgttcg
     1861 atccaatcca ttattactga caatacttat gttttgtgtt tactaaatgg attaggtcat
     1921 gaagatgtac tggaaaaata tgtgccaaag aaaaatatct tatttgggat cacgatgtgg
     1981 acagctggtc tagctggtcc cggaaaagtc acattgttag gtgatggaga aatcgaacta
     2041 gagaacttgg aaccagaagg cgaagcattt acaaaaaaag ttgtagaagt ctttcaagaa
     2101 gcaaatttaa atccgatcta tagccataac gttcgttatt ccatttggcg aaaagcttgt
     2161 gtaaatggaa cattgaatgg tttatgcacg attttggatt gtaatattgc tgaactaggc
     2221 gcgcaaaaag cggcagagag catggttcga acaatcgtat ctgaatttgc ttctatcgca
     2281 gcaaaagaag gaatcatact agatcaagaa gaagtgtacc aacatattgc ttctacttat
     2341 gatcctgaca acatcgggtt gcattaccca tctatgtacc aagacctgat caaaaaccat
     2401 cgtttgactg aaatcgacta catcaatggt gccatttggc gaaaaggaca aaaatatgat
     2461 attgctacac catattgtgc ctttttgacc caattagtcc atgcaaaaga aggaatcctt
     2521 ggcgcagaat aaaaggctct ctgatgagaa tcagctgaat cagcagctgg ttctcttttt
     2581 ttaagcccaa taaaagatca aaaagaaaac acaactctca tgatgccata agaattgtgc
     2641 ttgtttgttt cagtacataa tctcttcaaa atgtaggttt tttatttgtt ttcaacagag
     2701 gagaggtcat attcgtattg ctcataaata gtggaatcgt tagtgataaa tcgttttgcc
     2761 caattttctg catcataaaa ttccaatagc tctgatagga ttggcttaaa tggtgtatgc
     2821 agaaattccg gtaattcatc taaatcacgt tctcgaagca aaaaaatatt ttcaggacga
     2881 taaaaatgat aatgagcaat cgcttgatta tccgtcacta gtttcttttg aaaatacatg
     2941 gattcaaatg tccgcaaagt cactccttgc tgaccctccc gcaacagttc cagaatcccg
     3001 cgcgattccc ctgttttttt caggtactct tcataagtaa gatatccagg ttgttcattc
     3061 cctcgatgtg gaagaatgaa atagttggtc gtaacatctc tttttataaa ttcctgctta
     3121 tagctttctg ctcgttcttt tctcccatta ttcgttgcac caaaaaatag atccgtcttt
     3181 ggttcgctag aagaaaaatt catatgtttc gaataaaatg aactattatg tttcagccca
     3241 agttcttttg cttccaaaaa atcaaaatga taaatctcat cgattgccgg ttcatcttgt
     3301 gcctgcttca gcatcgcctc gtgttctgct ttgaagtgat tccaaaaaaa taacacaatt
     3361 tttgcattgg taaatgggcg cacgcgtcga gccatctcca tatcaaatcc ataatctaaa
     3421 aaaacgatca gctgataatt tgccaactgt tgtctagctg atttaatgaa ttccgactgt
     3481 agtaaatctt ttccggacca aacatctaaa gagcggatgg aaggcaaaaa ataaagtgat
     3541 ttattttttg caactagcag tacgtcattt ttgttcatct tttccgcctc actttctctg
     3601 tcactttttc ctatgatagc ctgtttaaat agtaaaaagc cagtaaaacg tttcctatat
     3661 ttgtatgaaa atttaacatt tgtatgaaaa tatgatgaaa taaatcattc ttctattaaa
     3721 attcgcctaa aagttttctg aatagttgta attgtctgaa aatttgttat cattagtcat
     3781 tgttaaatat tgctatttat tcttaatata aattttgatt gtgaatttaa aagaaagagg
     3841 tcatttatgg aagataaaaa attgaaaaaa caacttagct ccagacatat caccatgtta
     3901 gctttaggtg gagcaatcgg tgcaggtcta tttaaaggaa gtggcgaagc gattgggatc
     3961 gccggccctt ctgtattgat tgcttttttg attggtgggc tgattttata tatcgtgatg
     4021 aaaggtctgg gaaaaatcgt tttaagcggt ggcgacacgc atcacggatt atctggtttg
     4081 atacgccctt atctaggtgc tcattctgct gattttactg attgggtcta ctggtctatg
     4141 tggattatca atatcatcgc tgaagctgtt gcagctgctt cctttttgca gttgtggttc
     4201 ccgcacattc cagcctgggt atttgttttt atgttggctg ttttaactac gatcatcaat
     4261 ctttattcgg tacggttatt cgcagaaaca gaatattggc tggcttttgc taaaatcagt
     4321 gtcattatct tactgatcat ttttgctact tatcttgtcg gtcagcaaat gttgggcaca
     4381 ggcgttttcc ctacccttca gcaattgact gaccatggtg ggtttactcc tcatggtatg
     4441 aagggaatca tcagttcttt gcttgtagtt atctattctt acggcggttc tgaactgatt
     4501 gctatcacgg taagcgaagc agatgatcct aaacgtgcga ttcctaaagc aatcaaaggt
     4561 gtcatgggaa gaatcatttc cttttacatc attcctttgt ttttgctttt gattcttttt
     4621 ccttggaata ctttagcagg cacgaatgtc agtccttttg tgatggtatt tgaaaaaatg
     4681 aatatcccat ttgcagcgga tatcgtcaat tttgtgattg tccttgcttt gttctcatct
     4741 atcaattcgg gtgtgtatgc atcttcaaga attttgttct tccgtttgaa ggatcgaaaa
     4801 ggttcctcta gaaaactagc tgttttaaac aagcatcaag taccgcaacg tgctgtttta
     4861 ttttgtacca gtgttcttta tcttggtgta gctctttctt attttgtcgg tgacaaatta
     4921 tttggctatc tggcaggttc cctttcgtat acagtattat tgatctggat cattatcagt
     4981 gcggcatcct ttgtactggc actaagaaaa ggaacattat gggataaagg aatcagcttt
     5041 tttgcattag ctgtcttagg attgattttc cttggtatcc tgttcacgaa ctccattggg
     5101 gtaactgtat tgactggcct gctttacctg ttcatttatt tcagttatca aaaaaagaat
     5161 gatgcgttcg tattgacgaa tgaatgaaat cgatagaaaa ttttatttta tttatgtgga
     5221 atagatggag ctattgaaga aaggagttgt gacaaaagtc ttgttgcaac tcctttttcc
     5281 gaataaacag tctcatgacc tcttggtaaa cggtgttcaa aaaccctctg tgcaaaataa
     5341 ttgattgttc tccatgctca tgacatgata gaagaaagga atgaaaaaaa ggagtttgtt
     5401 ttaaatgaaa aagtattgga agtttttgtt gatgatcggt gtttcaacgg tagttatgtt
     5461 tggtatgatg tacttgaatg tctatgcgct cgatcatatc ttcttcagtc gtacacgagt
     5521 attcatggcg ttgatgatgg gtgcagtcat ggcagtcatc atgctgttgt tcatgtggaa
     5581 aatgtatgaa aacaaaaaga taaatgttat gatcttagcc gctagtcttc ttgtttttgc
     5641 cggttccttg tttatggtac gaagccaaac tactgtcaac gacacagctt ggatgaaggc
     5701 catgattcct catcattcta tcgctatcct ggcaagtaaa cgagctgatt taaaagatcc
     5761 tgaagtaaaa aaattagcgg aagaaatcat tcaggcacaa gaagaagaga tcgacaaaat
     5821 gaaaaaaatg atcaaagaaa tggaatgacc attatccgtc caaataaaaa aagaggatgt
     5881 tccagaagtt atcagcttca agaaataaga agaagctact tctggaacgc cgtgaataag
     5941 gggttgtgaa aaagctgccc tgcatcaagt aataagaaca gccaaacaaa aatagcttct
     6001 cctatttttc gtttggttag gcttattacc gcagatgcaa gcttttgaac accgtttatt
     6061 aagaggttgt aaaatcagcg ttctgacctg agtattagag agacaaatgg aaaaatagtt
     6121 cctcatattt tgccattttt tgagctaata ccgagggtca gctgattgaa caccgtttat
     6181 cgagaggttg tgaaaaagtc gcttagtttc gcgtattaag aaaaattttg tgaaatggct
     6241 ttccacattt ttgtataaat ttggcttaat accgagaaac tggcttttga ataccgttta
     6301 ttcgtaaaag ggattgtgac aaaagtcttg tcacaatccc tttttatgtt tttattgttc
     6361 taattgcgct gtcttgctta ctgcttcttg cggtttcaca aatttttttg agattcccca
     6421 acctgtgaag gcactgacca tcgaaaacac aggagaaagc aaactgaaaa atacgaatgg
     6481 caagtaagtg agtgtaggaa taccaaatgt acttgctacg aaacttccgg caattcccca
     6541 aggcactaag tagttgatta ctgttccacc atcttctagc gttctaccta atacagttgg
     6601 atcaaatcct gctttttcat aagtttcttt aaatgcatta ccaggcagaa taacagatag
     6661 gaattgttca ccaataaaaa tatttactcc gatacttgtc atcaatgttg taaaaatcaa
     6721 atttggtgtg ttcgtcatct ttcttgatac tcgttccatc acggttgaga tcaagccaaa
     6781 ttcgatcaac aatccaccta atgacaaagt tagaataatc aatgacacag taggcatcat
     6841 actttcgatt cctccgcggc taagcagtga atcgatttgt tggtttccag tatttgaaac
     6901 aaaccctgat tcgatgaccc cagccaactt cgttaaggaa gtttgtgggt tttgaataaa
     6961 aatcattcct actgctacag cgatattcaa caaaatcgtt aaaattgccg gcaccttttt
     7021 ccatgcacac agtaacatta aaaataaagg aatcagtgac cagattgaaa tagtaaagtt
     7081 tgcttctaga acatcagtca cttcacgaat agatgaaaga cttgcggctt tacttgtgtg
     7141 tcctaaaatc atgaataaaa agaaagaaac aacaaatgcc gggacagttg accacatcat
     7201 atgcttgatg tgtttgaaca aatcggcatc aacaatagct gctgctagat ttgttgattc
     7261 ggataatgga gacatcttat ctccaaaaac ggcacctgaa acaatagctc caacaaccag
     7321 cgctggattt aagcctagtg ttgtcccaat accgaaaaat gcaatcccaa tcgttgacat
     7381 aacagtaaag gcactcccta cagcgctacc tacgattgca cacacaacaa atacagaagg
     7441 gacaaaccac tggacactga tcaatttgaa cccaatgacc attagagttg gaatgatccc
     7501 tgcttgtatc catacagcaa tcaatgaacc gactaaaata aaaataaaga ttgggatgat
     7561 tcctggtttg atgccattga tgattccttc gtgcagccaa tctgtcgggt atctttttat
     7621 cactgcatac aacatgacca ttccgatcgc tgctaagatc gtgatattag gtgagacctt
     7681 taatccgaca acgccaatcg caatgcctaa aacaataacc attaatacaa gtgttgcttc
     7741 taaaaaactt ggtgttcctt tttttcccat gataatctct ccttttttag ttgttcttat
     7801 gttctaaaaa agttaagata tcggattact ccgccgcaat tcatccgcga ttcctccttt
     7861 ataaaaataa aaagacctgt tttaaactaa ggacgaatga ctccgcggta ccaccttaat
     7921 taaaaacaga tctgtttttc actttcaaac ttttattcaa atgtatggac tttccatgta
     7981 attcgtcaat taggcttgat cggttcacag cgaccaccga ctttctgaac aaaacaccta
     8041 accgctactg cgtgaaaagt tgacttgtac gaagtttacc ttccataaga tcatttgtca
     8101 atgaaaaaga aaatattttt tcatttttct gaaaattatt tttataactt tcacacacaa
     8161 gtttattttt tactagaaag tagcgccctc ttgttttttt cttgctacta tttcttgatg
     8221 ttcttgcttg attctttcta atagctttgg ctctttcaac agatcaagag ctgtattggc
     8281 gagtagttct gctgcaatcg caatagaagc aaggcctttg tcacttttag cagcagcttt
     8341 gaattcttct gtgtgtccgg caatatatgt ctcagagatt gctaccgtag gctggattgt
     8401 tggtatgact tggctgacat ttcctacatc agaagaaccg agacttgttc tttgttccgt
     8461 ttctatttct tcatctggaa cacctgcttc ttttgcatgc tggaagaata agtcatcaaa
     8521 agaaggagta acgatgacat catcaactgc attttggaac aatccaaact caaaagtcgt
     8581 gcccgtactt atcgcagcac ctcgtacgat attttccact ttttcatata ctttatctaa
     8641 tgtttgccga tttcctgcac gtaaatagaa acgtccagca gcgtaatctg gaatgacgtt
     8701 ggcagctact cctccgtctg taatgatccc atggatattg acatcttttg gcaattgcaa
     8761 gcgtagagcg ttgattccat taaacacttg gatcaatgct tccaaagcat ttactccttt
     8821 ttcaggtgct gctgctgcat gggaagcgac accgtaaaat ttgatgtcga ctggatcatt
     8881 cgctaaagat tctgtcgttt tcccataacg aaaagctgga tggacgcata acgctacatc
     8941 gacatcctca aaaaaacctt ctcgaacaaa gctgccttta gctgatccgt tctcgccccc
     9001 ttcttcccct ggggtgccat atacacggat ttctccacca gtttcatcaa tgaattgctt
     9061 cactatgctg gcggctaata cggagtaagt accaaacaaa ttatgcccgc atgcatgacc
     9121 aatacctggt aaagcatcga attctgccaa aaaggcaatt gtgggaccgg gtttattgga
     9181 aatataacgt gcatcaaagg ctgttcggtg tccagctact tctttttcta cccaaaacct
     9241 ttctttttct aattgtttaa tcaacgctgc tgaggaataa aactcatagt tacttacttc
     9301 aggatgttcg tggatatcta gagcaagtgc ttgatattct ggtaaacgat attggattag
     9361 atccttcaat tggtttgttg agattgttgt tgtcataaaa aattcctcct atgctagtca
     9421 tttttcttat tttctcccga atgtttccca tgcaggcacg cttgatcctt ttgatgtttt
     9481 ctcgattact ttgactgtgt cttctgtttg ataagcgtct actatttttt gatacgtttt
     9541 attgttttca tcttcttttc ttgcaacgat gatattcaca taaggacgtg catggtcatc
     9601 cactggttct aagaagatgg catcagaagc aggattaaat ccagcatcta cagctacacc
     9661 gctattgatc actgaaatgt ctacatctga caaagcacgt gctgtttggg aagcatctaa
     9721 ttcagaaatg tctagatttt ttctattctc ggtaatatca gaaacagtgg gcgcttgtcc
     9781 ttttgcctta tccactttga tcaatccagc agtttgcaat aacagaagag ctctgccgcc
     9841 atttgtaaca tcattaggga ttgctacttt tgctccgtct ttgatatcag ctacatcctt
     9901 caaagtgtta gaataaattc ccagcggtgc attgacagta tttccgatcg atactaaatc
     9961 agttccgtgt tctttattgt aattgtctaa aaagaattga tgttggaaag aattcaaatc
    10021 aatcgatcca tctgctaaag ccacatttgg ttgattgtaa tctgtaaatt tcacgtattc
    10081 caaattaatg ttctcctttt tcaaacggtc tttcacgttg tcccatacat ctgtatcttc
    10141 tccaataatc cctagtttta cgatagtttc ttttttgtca gttgtatctg cttttccttg
    10201 gctacaccct gccaaccaaa taattgctac aaatgtaatg actacaaaac gaataatttt
    10261 tttcattttt tctcctccac tttcaatgtg taattttttt aatcagttga ttactgataa
    10321 attgacttaa aaaaactaag attagaatca ataatgtagc cacaatcgtt acatctgttt
    10381 ggaatcgatt ataccctctc gatatagcaa gattccctaa tccgcctgca cctactgctc
    10441 cagccatggt agtcagtccg atcacgttga tgatagtcaa cgccgagacc cgaatgatac
    10501 caggcaaacc ttctaacaaa tacacacgta aaatgatacc gattggactg gttcccatcg
    10561 cttctgccgc ttcgatcacc ccaggatcaa cttctagcag tgcgttctcg atttgacgag
    10621 caaaaaacgg aacaactcca aaaatcaatg gcactaaagc tgctttcgca ccaatcgtcg
    10681 ttccaactaa gaaacgagta gtcaatgcta aaagagaaag caagataatg aaagggatgg
    10741 aacgaatgat attgatcagc ttatcaagta tttcaaatag cagacggtta gctagtaatc
    10801 cattagggcg tgtcactact aacgtcacac ctaagatgat tccaatcatc ccgccaatga
    10861 atgctgtcca aaaagacata tatagtgttt gcttcgtcgc ttcgacaaat tcatccggaa
    10921 tcaaataaac attgggcaaa tatgttttaa agaaatcagt cattttatct tcctccttcg
    10981 atcactttga tacgactatc taaatcgttt ttttgtaaaa tatttatttt tacaccagat
    11041 ttgatcaaat aatccaatgc ttttttccgc tgtttggttt ctccagctaa agtaacgatc
    11101 aaactcccta acggcgtttc ttgtaataac tctacattgc catataaaat attcgctgtt
    11161 acttgatatt tgctgtacag atgagcgatt agaggttcgt tcgtctgatt tccaatataa
    11221 cttaattcca ccagccattt attggcgatc tgatcggcaa aagcagaatg ggcaatgatc
    11281 gtttctaatg cttggtcaag atgagtagct gttcgaataa aatctttggt caattcttct
    11341 tcaggattgc tgaagatctg gatgctgcta cctttttcaa cgattcttcc atcttccata
    11401 accgctacct tgttgcagat ttctttgaca acttgcatct catgagtgat caggacgatt
    11461 gttaaaccta actggcgatt caattttttt aacaatgcaa ggatctgaag ggtcgttttc
    11521 ggatcaagtg cactggttgc ttcatcacat aataatacct taggttcatt cgccaaagct
    11581 cgtgcgattg ctactcgttg tttttgtccc cccgacagtt gtttaggata agcagatgct
    11641 ttttcttcca aaccgacaag ttccagtagt tcattcactt tttgacgacg ttcctgtttc
    11701 gattttccag aatactttaa agaaaaatca acgttgtcaa aaatagttcg gctttccatt
    11761 agattgaaat gctgaaagat cataccgatg gcctttcttt cttttcgcaa ttctttggga
    11821 gagagctgac tcagattcgt ctgattgaca atgacttctc ctgctgttgg tttctgcagc
    11881 aaattcatca cacgaactaa cgtacttttc cctgcacctg agtaaccgac gattccataa
    11941 acatcgcctt tttcaatcgt taaatctaca gaatctactg cctgtatctc tttttctttt
    12001 tgtttgaaac atacagaaac atctcgcaat tcaatcatta aaattcctcc ttatctaaaa
    12061 aaagcgtcct caagaaaatg aaaatgtcat tttcttgagg acgctttttt gcgtgttacc
    12121 acctcatgtt caagctgtgt tcacacacaa ctcctcttca agtacgatca tactctagca
    12181 agatatcggt cgcagccgtc atcgccttac atctatgctc gggatgaatt gctcgatggc
    12241 cattttccaa tagtcgttcc ttgcttcttc tcagctaccg aagctctctg tcaaaggttc
    12301 ttctatcgta cttaccattt caatgcaaat aatattcaaa aaaaaagtcc ttttactaac
    12361 ataaactatg tcagtaaaag gacgacgtat cgcggtacca ccttttttcg gaagtttcct
    12421 tccctcaagc ccaacatctc aaaaagagag attggcgttc gttttaacgg tcgaacagtt
    12481 ccgaatttgc ctacttatcg acaaattatt ctccaaggcc attttcagct tttcttactt
    12541 gactcttctc accagccaga gcctctctgc aaagtttcca aacttacttt ccttttcaac
    12601 gaaatcgata ttttgttgtt gggattaatc tacatgagga aaactgaaaa gtcaagtacg
    12661 aaattcaaat tattttttct atatttattt ttctgttttt ttatttaaca tttcaataac
    12721 agttcgaaga tcttttcctt ccgcaagaag tttcaatttt tccttttctt cgacggtata
    12781 atttcggaca aaactggaat caggtatcaa aacaacgcct aatcctgctc gtgaagcagc
    12841 tgttgctcct actaaagaat cttccactgc aaaggctgaa ctagcttcta atcctgttcg
    12901 ttccaaagct gtcagataag gaatcggtga aggcttgttc tccgacactt cgtctccgaa
    12961 tgtaaacgtg tccaagtaat cagctaattt aaagtgatcc aagatatctg ttgctctttt
    13021 cgtaacagtg gtcgtagcaa tgcctagttt catatgacgt tcttttaagt ctgccaatat
    13081 ttcttttgca tatggtttta agtggatggc tccgcttgtc agctgttgat aaaaatattc
    13141 ttcacgcttc gctcggactt cctgtacttt ttcagggcta cctgctgctt tcactaaaat
    13201 atcaaaactt tgtttccagt tttttccgcc ccaagcgtca acgatttctt ttgggatatg
    13261 gatatcaaat tgctgaaatc cccaaagcca gccatcacga tacgtttttt ctgtgtcaac
    13321 taacaaacca tctaaatcaa atattgcacc tttcatctat acattaatcc tttccaaaac
    13381 aatacacata gtttagcacg actggtccaa aaagaacaga agcaaagaac cagccttctc
    13441 gtttataaga gttcttggaa tcgacggata tagattttct tcacagctgt cacaagcgcc
    13501 agataagcga tgactgtagc aaataaccat aaccagtaat tgccaggaag tggcatcaag
    13561 cctaattcag caccaaaact tgtaaaagga aggacagaac cgacaataat tcctaatgta
    13621 gtgataacaa acatggcaaa tgttgcgttg ctttggataa atggtacttt cggtgtacgc
    13681 agtgcatgga gcactaaggt ttgtgtccat agtgattcga caaaccatcc tgcatggaat
    13741 aatgcgatga aaatcactcg ctgtgaacta tccaatgtat ggaaacttcc gcccactaca
    13801 gcaggacaaa taacaaaata catcaacaag taagtagtaa tatcaaatat cgaacttgtt
    13861 ggaccaaagt aaaccatgaa ttccccaata ctagatgcat tccatttttt tggctcgtcc
    13921 aaatattctt tatccatccg atcccatggc aaggaaatgc aggaaacatc atagatcaga
    13981 ttcaaaaaca aaagttgtaa aggcagcatt ggtaaaaatg gcaggaacgt acttgcaatc
    14041 agcacggaaa acatattgcc aaaattagaa cttgctgtag ccttgatata tttcatgata
    14101 ttcccaaaag tttctcgtcc agaaagaagg cctctttcca aaatcgtcaa atctttttct
    14161 aaaaggatca cgtctgcaga ttctttcgca atatccacag ctgtatctac agaaataccg
    14221 atatcagcca ctttcattgc aggagcgtca ttgataccat ctcctaaaaa tccgacagta
    14281 tgaccatttt tacgtaaagc tgtcgtcaaa cgtgcttttt gctgaggatt caattttacg
    14341 aatacttcat ggtcttcagc tacttgtgct aattcatgat cactcatttc cgagattttc
    14401 tcaccggtaa ccaattcatt gatctctaga cctacttgac ggcacactga acgtgtaact
    14461 aattcgttgt ccccagtcag aacttttaca gctacaccat gttctttcag agctttcaac
    14521 gcttgtttcg ttgtttcttt tggtggatca aggaaagcaa gataaccaat caaaaccatg
    14581 tctgactcat ctttgactga aaattctcca acgacgcttg gattggtctt ttgtgcgaca
    14641 ccaatcactc gtaagccatc ttcattaagt gactgtactt ttgcaagtac ggcttctttt
    14701 cgttcttttg ttaatggtgt aacagtaccg ccaagatcaa taaatttaga aatgttcagc
    14761 atttcttcaa tcgcgccttt tgtgatcatt tgtgttttac cagctggatc agcaaccaca
    14821 acactcatac gacgacgttc aaaatcaaaa ggaatttcat ccactttttg ataattgatc
    14881 ttatctgtat ctaaagtttc tgttgcttca tcaatgatag ccacatctaa caaatttttc
    14941 agccctgttt gatagtagct atttagatac gcatggcgca aaacacgatc atcttcattt
    15001 ccttcacaat ccatatgata ttcaagaatg attttatctt gtgtcaatgt ccccgtttta
    15061 tccgtacata acacatcgat tgcaccaaag ttctggatgg cattcaaatt tttgatcact
    15121 gttccttttt tggccatcac acttgctcct ttgaccaaat tcgtcgtaac gatcattgga
    15181 agcatttcag gtgttagacc aactgctaca gatagtccaa aaagaaaagc ttctagccaa
    15241 tccccttttg taagcccatt gatgacgata actgctggag ccatcaatga cataaatttg
    15301 atcagtagca tggatgtttt atggatgcct aattcaaagc ttgattgaat tggtttttca
    15361 gatagcgttt ctgctacatg accaaatagt gtttcatttc caacagaaac aatgattcct
    15421 tccgcacttc cactgatgac attacttccc ataaagacaa gattttcgta acttgtttcg
    15481 ctttgctgat cgtcttcaca tactttcgca tgcttttcta ctggatagct ttcccctgtc
    15541 aatgctgctt gggaaatgaa taaatccttt gcggacaata aacgcatatc cgctggtatc
    15601 atatcccctg ctgaaagccg aacaagatct ccacagacga tttcttccat aggaatctct
    15661 gtgtactttc cattacgttt tacagctgcc gttactttca caagtgattt cagtttttca
    15721 gccgcttgat tggaacgtac ggactgaatc aacgtcattg tcccgctgac gataaccata
    15781 gtgaagataa tggctacacc gaaaagatct ttatctccag gagctgctaa tacatattcc
    15841 gtgatgaatg aaataattcc taatgcgatc aacactaatg taaatggcgt gatgtatgct
    15901 ttcaatattt ctacaatgaa tggtgttttt ttgccatatg aaatcgcatt ttcaccgtaa
    15961 ttttctcttg aacgttcgat ttgttcttct gtcaatccat ttgctgatgc atggaatagt
    16021 tcaaataatt cttgtttcgt ttttccagca aaagctgcat agattttttg tgtatctttc
    16081 tttttttcca tctcaatctc tcctttgtgt ccaaaagaca gctgttctcg ctgcttttgg
    16141 tctttagcta ttcatcggat cgatagagaa aaaactgaaa ggggctgaga acagatagat
    16201 aatagccctg gcctttcctc tagatccttg tttttcgaat gactgtcatc catttgttct
    16261 catctccttt actaagtaat tttaattcat gacttccaat catttttcga atagttattt
    16321 ttccaattag accacctcct ttttattttt caccgatggg gatttgcggc aacaaaaaaa
    16381 cctctcgcca aatactcagc gaaaggtttg aaaaagcata ttaaaaaaag cttctcagat
    16441 ctcatcgttg agttttgaca ctgtacaacg taaaaagcag atacctgctt ttagctacct
    16501 tatgaaaagc cttatctcga caattcctgt tctacccatt ggcgtctctc gacgtttttg
    16561 ggcagtagcc tatgtttgta taggagcctc acctaacaaa tcttattcgg ttttccttcg
    16621 ttaatataac aactaaaaaa gaggatgtca aatcaatttt ccatgttttt catacttttt
    16681 ttaaaataca cacgttatat tcctgtctca cgcggtttta tttgtctcat tttccacctc
    16741 ccgctataat agaaatatag taggaaacga ggtgctgtta tggaaagaac acttgttatt
    16801 atcaagccag acggcgtgag acgccattta gtcggaagca tcatccaacg ttttgaagca
    16861 aagggactgg ccattgctga aatgaagttt gaaactatga ctcctgaatt agcaaaagaa
    16921 cattatcagc acttgactga gcgcagtttt tttgatgaac tgatcgatta catgacttcc
    16981 ggtcctgtcg tttatcttgt gctggtcggc gaggaagtga tcgatatcgt ccgtaagatg
    17041 gttggagcaa caaaagctgc cgatgctgtt ccaggtacga tccgaggaga ctacgcgctg
    17101 ccggggactg aaaacatcat tcacgcttct gattcccgtg atgctgcggt aaaagagatc
    17161 gcccgctttt tcccgccatt cgatataaac aaaccgtctg aaaaagcggc gaagtgatac
    17221 aaattctaaa ctgctggtct agttgctggg ttcagcaatg gccagcagtt ttttctctaa
    17281 ggacctccgt tgattctttc ctaacatata ttcgagcact gtgaagaatt cttggtagtc
    17341 tttcgtcttt tcttccccta aagcagcaga aatcaatctt tgataataat ctgccatcaa
    17401 tgtttcatag atcactgtac ttctttcttt atgtgctaag ctccgtttca tatcttcatt
    17461 cacactacgt gcctttttca accgaccatt ttctatataa aacgaagtca ggttcattaa
    17521 aaaagtaaag agatactgct catgattgtt caactgtgct aatcgtcgca ttcttttttt
    17581 gattttcaaa taattgttct caatgaaccg atcactgaat atccaaggaa aatcagctag
    17641 aatagatagt tcaaattgac catagtctgg tatctgcatc aagtattctt caatgattcg
    17701 gcattcataa cgtaaaaatt caaattctgt ctcagatact tctgtttttt cgaccatgtg
    17761 aagattcaaa accaaataat acagatcttc tgttttctta aaatgcctac ggttattgct
    17821 gatctcttta taaggatcaa agctgtcttc ttgtacccga tctagcattc gtcttttttt
    17881 ctttaacttt attggttcat ccagctggtt tcgcatccat tcatattctg caagtgtcat
    17941 gttcattcgc tctaaaaatg atctcaatgt tacaaatgac atttttttca ctgaatttgt
    18001 ctcaatcccg actagagaag tcttaaaaca gattccttcc gctaattggg actgtgtcaa
    18061 cttcttgttg actcgaagtt tcttgatcaa ttcaccatca tacataatag ttctcctttt
    18121 taatttaaat ttttccacat tcaataaata gatacgttaa aaaggaaaaa tacacgaaaa
    18181 aaataaaaaa atgttttttt cataaaaaat accaaccttc aagcattaga atgttgtcta
    18241 actactgaag gttggtttga ttgtttattg attatttttt tgctgttcaa cgtctgttct
    18301 tgttactgga tataagttgg ttgttgtttt attaccattt tttgaaaaga cacttgtcga
    18361 atgttcgcct ttttgctttt cgattgcttc attacgttcc gcttcatttt tatagtcata
    18421 ttttttcgga tcaatcgtct caaatccgtt tggcacataa aaccgcaaga gattttcctg
    18481 attcagctta tctgaaagac ttaacgcagt cttgactttt tcctgatctt tggagatttc
    18541 ctcatcgatt cctgctgctt tcgtatcgat gatttctcct gtctgattat tgtaagcttt
    18601 tcctcctagg acggtgtatt tgggtgtcac aaagttccca tttcgaaaag caactacttg
    18661 atcatgctga gaagataata agtctgtacc aaaatgaaga taatctttat catcgattcc
    18721 caatagatgt agtaatgtcg gcaaaacatc gatttctcca ccgtattcat gattgactgt
    18781 ccctttagta taaccaggca tatggatcat caatggcact cgttgcatct gtgcgttgtc
    18841 aaattcatcc catgtatctg gatctttacc aagagcgctt gctaaatctt tattatctgt
    18901 attcgagatc ccaaaatgat ccccgtaaat cacaaagatt gaattttggt aaataccaga
    18961 ttttttcaaa tacgtaaaaa attgttccaa tgattgatcc agataatgtg ccgttcgaac
    19021 ataattatct actgtgctat tgccagtatt caaagatgga aagtcgaaat tcttgtcatc
    19081 cgtataataa ggcgtgtggt tggttacgga aagaaatttc gtgtaaaacg gctgttgtag
    19141 atgctcaaga tattgggcag attctctgaa taaatcttta tctaatatcc cataacctaa
    19201 cgtttcgtcg gattcatcaa aataagatcg atcaaagaaa ttgtcataac ctaagttttt
    19261 atatacatga tctctattcc aaaaactagc tacattccca tggaaaacag cgcttgtata
    19321 gccttgcttt tgtccaagga tcgctggtgc ggcttgaaag acattgtctg acccaagttc
    19381 tgtgaataat gagccttgag gtaaaccaaa tgtgccagtt tccaacatat tttcagcatc
    19441 acttgtcttt ccctgtccta cttcatgaaa gaaattgtca aagctgatcg ttgcttgatt
    19501 ctggaaaata ctatttaaaa acggtgtgac ttcttgtccg tctaccttca tattaatcaa
    19561 aaactgctgg aaactttcta aatggagaac gatcacattt ttccctttag cgatcccaaa
    19621 tgtttcggga tttggctctg cataatgttt ctttgtataa tctaaaactt catcaatccc
    19681 attactgctt gcatgcgcgc gaacactgct agtcattcct gttttgattc cgtcataaat
    19741 agtatacgca tcgattccta gatatttgac aatatatgaa cggtcaaatg ttcgctgcag
    19801 caattgtgga cggttggctt cgctcagcgc tagatttcct gaaaaaataa gccctgaaag
    19861 acaggtaaca gcgatagcca tcggtttgcc gacagggttg cttttgatag gtattttctt
    19921 gaccttcaac caaaccagta gcccaatgaa cacagcgata tccagccagt agatgacatc
    19981 atggggcttc atcaaagaaa aggagcttcc tgacaatcct tgcgacactt tcgaaaagcc
    20041 taatacagat ttgactgtaa tgaaatctgt gaattcccga taaaaaataa tattcaagta
    20101 caacaaaagc gtatttaaaa tatcaatgat caaaagaata gctaacgctg gctttggctt
    20161 tttaatatac aaagaaaggc caaaaaacaa taaggtagtc gcaattgggt tgatccacaa
    20221 gataaagtac tggatcgtac cttccacacc taatgaaaaa tctgcatagt aggcaatgat
    20281 cgtcttcagc cagaaaaaga aaacagctgt caaaacgatt gctaaacgat tttggcacaa
    20341 ttttttaagt tgtgtcacgt atatcacttc tctctattca cattttttct tattttgctc
    20401 ctgaaaaatg aaaatttcct aaaaaagttg ttatcttaag atttttttat tctttctaaa
    20461 gattattccg tccacttttc acagcataaa accgctgcga tgtaagcgat taaactaatg
    20521 aagagataaa agtcttgtgg tgaccgcttc actccacggt attcaaaagc cagtttctcg
    20581 gtattcgtac aaaaaaagag gctgtgacaa aattcttgtc gcagctcctt atttcttgat
    20641 gcaggacagc tttttcacaa cttcttcctt aaacggtgtt ctcctgccaa ctcctcggcc
    20701 acaagtcaca atatttataa aacatgagaa gctgtttttt gaaattgttc cttgtggctc
    20761 agagttaaac ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccctcgg
    20821 tattagatta tgtcaggacg ctaattttac aacctcttgg taaacggtgt tcaaaagctt
    20881 gcatctgcgg taataagccc aaccaaacga aaaatataag gagctatttt ttgaaattgc
    20941 tccttgtggc tcagagttaa acggcaggct caagacctct tgata
//
LOCUS       DACCKU010000050        20942 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.052, whole genome shotgun sequence.
ACCESSION   DACCKU010000050 DACCKU010000000
VERSION     DACCKU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.052"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            168..362
                     /locus_tag="IVN13_001702"
     CDS             168..362
                     /locus_tag="IVN13_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012195.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            631..930
                     /locus_tag="IVN13_001703"
     CDS             631..930
                     /locus_tag="IVN13_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012196.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1027..1461)
                     /locus_tag="IVN13_001704"
     CDS             complement(1027..1461)
                     /locus_tag="IVN13_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012197.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1693..2403)
                     /locus_tag="IVN13_001705"
     CDS             complement(1693..2403)
                     /locus_tag="IVN13_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP7012198.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2393..3253)
                     /locus_tag="IVN13_001706"
     CDS             complement(2393..3253)
                     /locus_tag="IVN13_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP7012199.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3258..3896)
                     /locus_tag="IVN13_001707"
     CDS             complement(3258..3896)
                     /locus_tag="IVN13_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP7012200.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3911..4210)
                     /locus_tag="IVN13_001708"
     CDS             complement(3911..4210)
                     /locus_tag="IVN13_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012201.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4253..5737)
                     /locus_tag="IVN13_001709"
     CDS             complement(4253..5737)
                     /locus_tag="IVN13_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP7012202.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5757..6218)
                     /locus_tag="IVN13_001710"
     CDS             complement(5757..6218)
                     /locus_tag="IVN13_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7012203.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVN13_001711"
     CDS             complement(6236..7303)
                     /gene="ulaG"
                     /locus_tag="IVN13_001711"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP7012204.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7521..8291
                     /locus_tag="IVN13_001712"
     CDS             7521..8291
                     /locus_tag="IVN13_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7012205.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8564..9391)
                     /locus_tag="IVN13_001713"
     CDS             complement(8564..9391)
                     /locus_tag="IVN13_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7012206.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9959..11194
                     /locus_tag="IVN13_001714"
     CDS             9959..11194
                     /locus_tag="IVN13_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP7012207.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12193..12453)
                     /locus_tag="IVN13_001715"
     CDS             complement(12193..12453)
                     /locus_tag="IVN13_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP7012208.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12480..13781)
                     /locus_tag="IVN13_001716"
     CDS             complement(12480..13781)
                     /locus_tag="IVN13_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012209.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14073..14531)
                     /locus_tag="IVN13_001717"
     CDS             complement(14073..14531)
                     /locus_tag="IVN13_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP7012210.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EETLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14544..15869)
                     /locus_tag="IVN13_001718"
     CDS             complement(14544..15869)
                     /locus_tag="IVN13_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP7012211.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15886..17298)
                     /locus_tag="IVN13_001719"
     CDS             complement(15886..17298)
                     /locus_tag="IVN13_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7012212.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17315..18079)
                     /locus_tag="IVN13_001720"
     CDS             complement(17315..18079)
                     /locus_tag="IVN13_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP7012213.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18183..19058)
                     /locus_tag="IVN13_001721"
     CDS             complement(18183..19058)
                     /locus_tag="IVN13_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7012214.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19313..19397)
                     /locus_tag="IVN13_001722"
     tRNA            complement(19313..19397)
                     /locus_tag="IVN13_001722"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19361..19363),aa:Leu,seq:caa)
     gene            complement(19415..19486)
                     /locus_tag="IVN13_001723"
     tRNA            complement(19415..19486)
                     /locus_tag="IVN13_001723"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19452..19454),aa:Gln,seq:ttg)
     gene            complement(19496..19568)
                     /locus_tag="IVN13_001724"
     tRNA            complement(19496..19568)
                     /locus_tag="IVN13_001724"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19533..19535),aa:His,seq:gtg)
     gene            complement(19630..19714)
                     /locus_tag="IVN13_001725"
     tRNA            complement(19630..19714)
                     /locus_tag="IVN13_001725"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19678..19680),aa:Leu,seq:caa)
     gene            complement(19735..19805)
                     /locus_tag="IVN13_001726"
     tRNA            complement(19735..19805)
                     /locus_tag="IVN13_001726"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Cys,seq:gca)
     gene            complement(19851..19922)
                     /locus_tag="IVN13_001727"
     tRNA            complement(19851..19922)
                     /locus_tag="IVN13_001727"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19888..19890),aa:Gln,seq:ttg)
     gene            complement(19934..20006)
                     /locus_tag="IVN13_001728"
     tRNA            complement(19934..20006)
                     /locus_tag="IVN13_001728"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19971..19973),aa:His,seq:gtg)
     gene            complement(20009..20082)
                     /locus_tag="IVN13_001729"
     tRNA            complement(20009..20082)
                     /locus_tag="IVN13_001729"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20048..20050),aa:Trp,seq:cca)
     gene            complement(20089..20169)
                     /locus_tag="IVN13_001730"
     tRNA            complement(20089..20169)
                     /locus_tag="IVN13_001730"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20133..20135),aa:Tyr,seq:gta)
     gene            complement(20171..20246)
                     /locus_tag="IVN13_001731"
     tRNA            complement(20171..20246)
                     /locus_tag="IVN13_001731"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20211..20213),aa:Phe,seq:gaa)
     gene            complement(20282..20354)
                     /locus_tag="IVN13_001732"
     tRNA            complement(20282..20354)
                     /locus_tag="IVN13_001732"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20319..20321),aa:Asp,seq:gtc)
     gene            complement(20364..20436)
                     /locus_tag="IVN13_001733"
     tRNA            complement(20364..20436)
                     /locus_tag="IVN13_001733"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20401..20403),aa:Val,seq:tac)
     gene            complement(20452..20523)
                     /locus_tag="IVN13_001734"
     tRNA            complement(20452..20523)
                     /locus_tag="IVN13_001734"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20488..20490),aa:Glu,seq:ttc)
     gene            complement(20538..20627)
                     /locus_tag="IVN13_001735"
     tRNA            complement(20538..20627)
                     /locus_tag="IVN13_001735"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20591..20593),aa:Ser,seq:gga)
     gene            complement(20667..20740)
                     /locus_tag="IVN13_001736"
     tRNA            complement(20667..20740)
                     /locus_tag="IVN13_001736"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20704..20706),aa:Asn,seq:gtt)
     gene            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="IVN13_001737"
     rRNA            complement(20750..20865)
                     /gene="rrf"
                     /locus_tag="IVN13_001737"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6602 a         4392 c         3330 g         6618 t
ORIGIN      
        1 taagaacaat agccttttat tatatatatt attcaaatta gttatatcga gtgaaaaaaa
       61 ctttcaaatt gattcttttt atttatcata aagatataga tacataatca ttttcactta
      121 catatatttg aatattaact aaaaacagca agaaagaaga ggatcaaatg tattatgtag
      181 aagtaaaaac aaaaggcgtc aaaaacaaac aatatgtgaa aggtatcagt aacgaatatc
      241 ctttacttgg ttcatggaaa gaagctgctc ctttttccaa accatgtgcc ataaaaataa
      301 agaacgagct agaaaaagaa ctgacttgtg gtaaagctgt tgtagatatc atcgaaaagt
      361 aaatccaatg tatagaaagg aagatcacac atgaaaagcg acgtacaaat tgctaagatt
      421 atagaattag aagaagaaga cgcagtgttg acaattgaaa aaaaataaat gtttgtatac
      481 catatgatta tttcaatatg tatcctggaa ttggagatct cgtagagcta tatcaagatg
      541 aatcaaatat acttgtcatt catcatgaaa tatagagact tataaaattg tttattttta
      601 taatgtatca gtatagaaag gaaataaaac atggattata ctgttgaatt tgacagtgaa
      661 ctacttaagc aatttccaca agaagaacat ttcatgtata aagaagcaat ttcccacaaa
      721 gtgacagagt atttggagca taattttttt cgtataaagc cagtgcgtac tcgttcggca
      781 aaaggaatat acgagatgaa agtcaaaatt aataaaaaaa attatcgcat agcatttgct
      841 ttaaaaaaaa atcatgtgtt cattttttat attagttcga atttgcaaaa aattgtattt
      901 gataaagagg tgagtaaaaa aataaaatag ctgagcattg gaggatagat tgagtgatac
      961 taagtctatc ctcttgtttt tctaaagaac taagctagaa ttttttcttt tgtacgttat
     1021 tatatgttat ttgtcattac caaaaagcac cttttttctt ctagtatcta tgacataaga
     1081 aataccaccc atttccttta cttgaacaaa tcctactgat tcataaaatt tttttgtttt
     1141 ctcagtatca tctgtcaata attcgatttg gactggtcca taactagtga tcttctccaa
     1201 agcttcattc aaaagcactt tgcctattcc ttggcgctga tacctcggat gaatgaggat
     1261 atcttgaata aatgcgatcg tcgcaaaatc agtgatccaa cgaacgattc cgataatctt
     1321 cccttcccca ttacgcttta tcagagattc agattgttca aatgctctag ctaaccttgc
     1381 agtgtctttt gtgtaattcg tccaaccaac gctcttatat aattcagaaa tctcatcgat
     1441 cataagggaa tcaatattca tttttttctt cctttcttat acaattctcc ctctaataat
     1501 acagttcttt gactatttaa tctatcacat ataactctcc tgtaaagatt ctctcgattc
     1561 cttaaaattc ctctttcctt ttagaatgca tactttttga taaataaaaa ggttgtgata
     1621 aattttcttc acaacctctg tttctctcta tcaagtgtta tcttttaatt ttctaccctt
     1681 accctagcat ctctattgcc cgtagtatgc atactttcca tgtttgcgaa aataatgttt
     1741 atctagtaaa tattgcggga tttcacatat attggggttg atcagctcgt tttctttggc
     1801 catcagacaa atttcatcta agatcaaact gttttctact gctttcatag gtgaatcccc
     1861 ccatgtgaac ggtccatgtc catagacaag tactccaggg acttcgtttg gatctaattt
     1921 tctttcctta aacgtctcga caatcacatt ccctgtatga acttcgtaag cttctcttac
     1981 ttcttctttc gttaattggc gtgtacaagg aaccttacca taaaaagcat cagcatgagt
     2041 cgtgccataa gctggcaaat cgcgtcctgc ttgcgcccac atgacggagt gcgtggaatg
     2101 agtatgagta atagcagtta tatcttcaaa agtttgatac aaaacaacat gtgtggggag
     2161 atctgaagat ggttttaagc tatcttcttc tattatattt cccgacaaat ctgttaccac
     2221 catttgatct gcttgcatgc attcatatct gacaccgctt ggcttaatga cgatgactcc
     2281 tagctcacgg tccacttcac tcacattgcc ccaagtcaat ttgactaatc cgtatcgcgg
     2341 caatgccaga tttgcttcaa ggacacgttg cttcattgct gcaatttttt cttcacaaga
     2401 catcatagcc agcctccttc aatttagaac ttaagtagtc ttttgcctgc tggatttctc
     2461 ttcgaaagtc ttggcttttt tcactccaca tttcaatcag aaatgttcca tcataatcta
     2521 gacgtttcaa atgtcttaat aatccaagaa aatcaacaca accgctacca aaaggaacat
     2581 cacgaaactg gcctgaatat tcttttgtta cagggtaagt atcttttaag tggatagcag
     2641 tgatataatc gatccctttc tcaagttctc gagccggatt attttccggc catgcggaca
     2701 aattgcctag gtctggatat acttgtagaa aaggtgaatg aatctgttct ttgatttcta
     2761 aaaattttga aatggagttc ataaatggat catccatgat ttccacggaa agaatgatgc
     2821 catatttaga agcttccttt acgctttctt ttattccttc gataaagtct tctctggaat
     2881 tcattgattt ctcttcataa taaacatcgt atccagctat ttggattact ttgacgctta
     2941 gattatgcgc caaatggatt gcttttttca ttagttgttt cgctgctttt ttcttttctg
     3001 gatcagcaga accaaacgga aatcgccgat gcgcacttaa acaaatcgaa tggatacgta
     3061 caccggagct gaacatctct tctctcagct cagctatctt ttcttcagac caatctaatc
     3121 gtgctaaccg ctcatccgtc tcatcaatcg acatctcgat aaaatcgaag tccatttctt
     3181 ttacaaggga aaaacgttct ttccatgaaa tatctttggg taacgctttt tcataaatcc
     3241 caatttgagc catttcctta cccccagata cgaatgatct catctttgaa gttttgtgcg
     3301 gccttcatcg gatcatttgc tgctgtaata ccacgaccag tgataaaggt atacacatcg
     3361 atcccttcaa ataagtgtaa tgtgtgtgga tttaatccac cagttacaga aacattaaag
     3421 cctagttcaa tcagtttttt gatcttcgat aaatcttttt caccccaagt ttcaccagat
     3481 aacagcgcat cacggctttg atggtagatt acctgccgga taccgatgtt atgccaatcc
     3541 atggcttgtt caaaagtcca attgccgtag agttctactt gcaattcacc tatttctttt
     3601 tgtgctgcct ccatagttgg aatcgttgca caacaaatga ctgtcatcca gtctgctcct
     3661 gcttttgcaa cattactcgc cactgttcct ccagcgtctg cacatttcgt atcagcgact
     3721 agttttttat ctgggaacat actgcgaata cagcgaatcg ctttttgtcc ttcctgtaag
     3781 caaagaattg tgcccacttc aataatatct actacatcac caactttcat aacatctcct
     3841 aatgcatgtt ctaagtcatt gtgatctaac gcaatttgca aattcggcct gctcatctta
     3901 gttccttcct ttacttgttt ttttggtacc actttgtttc agcaaatttt tcactgattt
     3961 ctttgactga catcacattt ttgataccga taactgtaac tccttttttc tctgcctgag
     4021 taaacatatt caaaaaattg actggcgtaa aaactacatc gtattggcca gctgaacttt
     4081 ttccctctga aatggcacaa tgatggattt tggtaattgg tacgtttagt tctttcattg
     4141 ctttttccac acttctcatc atcatcaagc ttgttcctga tccatttgca cacgatacta
     4201 aaattctcat cttaatccct ccattatgag tgttgtctaa tcagattttc ttttatactc
     4261 tctgcttttc tgcatatgtg tcatagtctt ctacaatcaa aaaataacca tcaggatttt
     4321 tccgatattg taactgcgga atagccaata aaccaatgac aactacggca attccagcat
     4381 aacctaggac tttcatgatt actgtaaaca atggccaagc tgttgcccag tcgaacatcc
     4441 cgatataacc accgtatgtt gacatcccaa tccatgtagc gaataatgct gaaccgccta
     4501 cttgtatgat tcctgaaaga aacgggaaca ggcaagcggc tttgaatcca cctcggttat
     4561 tggcataaac tgctataacg gcattatcaa agaacaacgg aatgaaaccg gctatgacga
     4621 tcgttggtga tttcaataaa atcagtaaac caatcatcaa aaactgtccc aaagcaccaa
     4681 acaagaaccc aatcgttacc gcatttggcg aaccaaagcc aaaagtagca gctacgtcaa
     4741 ttcctggaac agctcctggt aaaatcgtgt ttgagattcc ttggaaagat tgagttagtt
     4801 catcgacgaa tgtccgaaca cctaattgca agatcgccag atataccgca aaatttaaag
     4861 aagtcgtcat aatgtagaat aagaaactct gcccttcttg catgaattgc gcttgaatca
     4921 aataatcttt gcctagtaca atcagaatga tcccaaagaa aaatagcatg agtaacgaag
     4981 tagcaaccat attctcattg aaaatcgaca agaatcctgg taatttgata tcttcaattt
     5041 ttttgtcttg cttgattgat ctatttttct tttcatcccg ctttttcatc cactcagcta
     5101 attttgcaaa tataaagatt ccgaacattt gctgatgtgc cactgcaaat ccggcacctt
     5161 ctgtcaattc ctgcgtgatt cctactgtta agttagaagc tactgcccaa taacaaccta
     5221 aaatcagtcc cataaaaatc aatacttgta tcctacctag atcaggaaag caaaacagta
     5281 aaatccaaaa agcggtagct gcttgttgaa cctgaacgtt tccggtagtg aaaacggaac
     5341 gcagtttcgt atatttctga aaacggacta ataagatatt taaaacaaat gcaatcagta
     5401 ataaaatcat cacatctcca aaggtacggc caaatgtctc cattaatcca gcatccacag
     5461 cattttgtcc aaaataagga tctgtaacca tggcatccaa attgaaccgt tcctttagtc
     5521 caaccaagat tggccgaaaa ttattgacca aaccaccaga gccaacagtc aaaataaagt
     5581 aaccgaccgt tgcctttaag aatccagcga tgcattcatg aagcgggcgt ttcagcaaaa
     5641 tatagcctaa taacacgata aaaccaatca ggtatgctgg ctgcgtcaag atatttgttg
     5701 caaaatacgt ccaaatgctt aacaaaaatt cccccatctt ttttctcctc tatcaattaa
     5761 ttatattctg ccatcacaac ttggtaatca tcgatggagt tcgtagagat gagacgctca
     5821 accaatccat ctgtcattaa taattcggat aatttttgga tattttctag atgttcttct
     5881 ggatcttttg ctgcgagtgt aaaaaacaaa ctagccgttt tctcctcatt ctccccttca
     5941 ctttcaaaac ggattggctg tttcaacttg gtaaatgaaa tagctgttcc aaaaacacca
     6001 tcactttttt ctgaagaatg cggcattgct acttgaggtg cgatcacaat ataaggaccg
     6061 tatttttcta cacaatggat gatctcctcc acatacgttt catcaataat ttccttatcg
     6121 atcaatacaa gacagctttc ggcgatagct tcacgccaat ccgtgatttc tttatctggg
     6181 taatgaatca attgatggtc aaaaaagtat ttcagcaaga aactcccctt ctttcttata
     6241 aaaacgacgg aaacggtgta tcattttcaa tcgagaagac atcttcaaat cctcgataaa
     6301 aattaaactc aagatcatct ttgtttgtcg gatagatgaa tttcccgcct acttgccaga
     6361 tataaggttt aaatccatac ttcaggcgat cctttttgaa tttccaaatt tcagtgatct
     6421 cttttggatc agccataaaa ttagaccaaa tatcgtagtg gacaggaatc actacttttg
     6481 cattcaatga ctccgccatg cgcagcatat caacagaagt aactttgtct gttatccctc
     6541 gtgggttttc accataggcc cctaaacaga catcgatttt gtgttcatta ccatgtttgg
     6601 caaataaatt ggagtaatgt gaatctcctg catgataaag attccctcct gatgtttcga
     6661 ataaatagtt tactgcaatt tgatccatct cctgaggcat ttttcctttt aatacctctt
     6721 ctggatcttc acaagtgacc aacgctgtcc gatcaaacgc ttctaaagct actacattga
     6781 tgtctttgat tttgactgca tcacctggct ttactgtaat cgttttttct tcaggaattc
     6841 cccatttttg ccacgtgcga accacttctt ttggcccaat gaatgttgct tcaggacaat
     6901 tttgatgaac tgctgcggct gtatggatat ctagatgatc ggaatggata tgtgtaacga
     6961 ttaaagcgtc tacatttttt atcgcaaaag gatcgattac gaaaggctga gtacgtaaat
     7021 taggctgcat attttgaacg ccactcatac gcatcatctg atggcctttt ttcatcatgc
     7081 cattcccatg actacgtttt cccgtcccac accatagatc acataaaata ttcgttcctt
     7141 catgactttt cagccaaatt cccgtacagc ccagccacca catagcaaaa gttccaggat
     7201 ccactacttc tgtttcgatt tcttcattca agtatgtgcc ccactcagga aatgtttcta
     7261 ctaaccactt ttcttttgtt acttcattga cagttttact catttgcttg ctccccccat
     7321 atcgttgttc caacttacaa gtaaatatta atgtgtatca gcatccatga aaaccctttt
     7381 atatgaccgt ttgtttttta tatgattttc tttcagtaat caatgattgt ttcagtataa
     7441 taaagacaga tatgaatgtt taaatgaaca actagtcaaa taaatccatg gatcaagatg
     7501 tccctcagga ggcaaaagtc atgaaaaact cacttgtcaa tattgaaaaa cgtcaaaaag
     7561 aaattttgga actgcttcag cagactgaac aactatcaac tttcgaatta gctgaatccc
     7621 ttgatgtatc tattagtacg atacgaagag atttaaattt attagaagaa aaaaacgaga
     7681 tcatacgcaa ttacggttat tgttcttata attacaaaaa ccagactgat tttgatcaat
     7741 ctggtcctga aaggattaaa caagcgattg ccagagaagc tagtaaatac attaacgatt
     7801 atgatacgct gtttatcaat tctagctcga ctgcgttgaa aacactggat tttcttcaat
     7861 ctaaccattt gacagtcatt acaaacaacg tgaaaatcaa ttactctccg cacaaagata
     7921 attgtagtta tattttaact ggtggagaga tgcgctttcc aaaagaagca ctagtgggcg
     7981 acatcgcatt aaatacgatt tcttcagtca atgcagacat ttgtatcatt ggctgtagcg
     8041 gagtcgatgt agtacatggg gtcacaacaa agatattaaa tgaatcaacg atcaacgaaa
     8101 tgatggtcaa caagacaatc aaatgtaaga ttttagtcgc tgatcatcga aaaatcggac
     8161 tgacatcaaa gttcaagatt gctgatattt cagtctttga ttatttgatc actgataaat
     8221 tttgttctga aaaagtatta gaggagatca gaaatacggg gataaaaacc attcaagtag
     8281 attcatttta agaaccagga cgtaaatatg taaataggat tatcaaaaaa atgaataaca
     8341 aatttatcga taaatgaaaa acacgctacg ctcaaattga taaaggaatc ccaagatgta
     8401 tccatccaat cgataccatc ataatctatt gcttctttta taaccgttat atgttgccta
     8461 aataggaatc aaacaatatc gaaaaaagga ggtatgacaa aagtcacacc tccttttttc
     8521 gaataaacgg tattcaaatc agaacactga tttcacaacc actttatttc aggtcgtttt
     8581 caatcacctt taccgcgtaa tcatttccgt tgaaacgcgc tatttttttt gcgtccatat
     8641 atctttctaa ataatcatct tctcctaatt gcttatggca agaagcaatt tcataaagca
     8701 agtattccaa tgcataagtc gatttatgtt tcttattata ctcaataccc ttctcacata
     8761 attggattgc tttatcataa tcctcgattt tacggtaata ggtggcgata tcataatata
     8821 caatgcagac catcagatta tcaaaatcag tctcactcaa tattttaagt gtacgctcca
     8881 cataaacttt cgcaaaatct aattctcctt ttgacaagta gatcattgcc acgtttgcag
     8941 ctgcttgtaa gtgatggata tctgtttcgg tcgtttgttc caacacttga ttaaaataaa
     9001 ataatgctgc atctggtttt ttcgcataaa gatgatcaag cagtccttga taaaataaaa
     9061 tttgatttaa aagatcagaa ttacttactt tttcctgatc gatcgtttgt aaaattttat
     9121 aagattcttg gtattttgat acagaattca atgattgaat ctcatttatt tttttcctga
     9181 ttttgtcttc ttctgtttct actaatatat cggagatctc gacattcagc tttttgcaaa
     9241 gcgcacttaa gatattgatg cctggcgaca tattcttgtt ttcgatattg ctgacagttg
     9301 cttgttcaca aatgccttct gctaattgta cttgtgttaa cccctttttc tttcggtatt
     9361 cttttagtaa tgctccatct actttaatca aaacaatttc cttcccttta aaaaattatt
     9421 tctcctttat atcatacaca aaaaaaagtt ctaaacaacc aaaaatgttt agaacagaat
     9481 aaaaatttta attttaaaat aatattcaaa ttaaacaact ggcgtatctg ctccttgatt
     9541 tctttttgtg ttatctgaat aattatcgtt tacagttata acacatagtg aaaattgtca
     9601 tggcgagaaa gtctaagaat atttttaaac ataactattt ctttcttacg tgtgtataat
     9661 gacgacagca cacctatcaa ttaatataat tattttattt ttttatgttc tcatgtttat
     9721 ttttaatgac acaacttctc gtatttatct ggcacaacat ataaatcgat caaaaaattg
     9781 ttgcgtgttt ttttgaaaat tggagtttca acttgattac aaaatttaaa tttagaagtt
     9841 gtgaaaacag cgagagacag tttaatattc aaaatataca taggctactc gaacgctgtg
     9901 ttttttagta tactagtaag aaacgaaggg atagactcat tccaataagg aggatttcat
     9961 gaagacaatt ctttttgctc ctgcagtatt caatttagct gagacaacac gaatgattga
    10021 ggttgccaaa gctatgaaag accgttatta ctgtgtattc tttggctttt ctaaaacttt
    10081 tgctcctttg atcacagaaa gtggctttga gtatcattta ctagcgccga tccttacgaa
    10141 aaaacaagaa gaacaaatca taaaatttga ccagttgcgt acacttaaaa acccttttac
    10201 caaagaaatg gttgcctctc gtatcactag tgaacagagt ctgattgcta agtttgagcc
    10261 tcgactgatt gtgacaggtt cgaatgtgac aattttttta tcggcccgca ataaaaagat
    10321 tcctttagta tatgttaaac cctatgcttt gagtgaacct ttttttcaat cagagcatgc
    10381 gctgatgccc cacgagttac gtttcttcgg ccctttaagc aatccccttt ggcactggac
    10441 aaaaaagcgt atagtaaata cccgatggat ccccaatgcc ttcaaacacg ctgcacaaaa
    10501 agaaatgctt cctttaccaa aaacaagttt acaaatgatg gatgctgatc taaatctcat
    10561 cacaacgcct aaactatttt tggaagaaga acagctgccg gaaaattatc gaatcgttgg
    10621 tccaattttt gctaagctca gtacttcctt gcctgaagaa gtgctaactt ttatacagcg
    10681 aaagcaacaa gaaagaaaac gtattatcta tttcgcaatg ggtagttcag gcaatccgcg
    10741 tatgttacgc aaaattttgt tcttcttaca aaaacaaccg gatctagcgg ttattgcacc
    10801 tattgactat ctgcttgatg acattcaatc tgactcagaa gatcttttaa taacagaata
    10861 tctgtctagt catcttttgc atgaattcat tgatttttct tttatccacg gcggggaagg
    10921 gaccgtccaa actgcctgtg aatccggcaa accgtttatc ggtgtgggga tgcaatttga
    10981 gcaaagctgt aatattgact attgtgtaaa atacggaaac gcaatcgccc tcacaaaacc
    11041 agtgactgaa agaaaactgc agattgctct gcgcgaagtt tcttcaccca agattcgtca
    11101 gcaggcacgc acgctgcaag ccgtttttcc aataaatggt ccacaacaag caattcaaga
    11161 aatcgaaacc ttcctgagca accacccaat atagtttcaa gtagaaaaag cttttttaat
    11221 agagactcat cagtaatttc gctattcgag acccagacag tgaaaataca aaaaaagtaa
    11281 atagtggatt gaaaagttag agttacttaa attacggtgt ctaaaaaaca caaaatcatc
    11341 cttaaaataa aagatctgac gctatccaaa tgagcatagg tagcgtctgc catttattct
    11401 tagtcaaatt ctttgacacc tttctctggt tggatcatct caacgtttct tcaaaagaaa
    11461 gaccataata attaggcaaa tacattttct taaaatgtgt catatagatc tagaagcatc
    11521 gctttgtaga gatttgtgcc gctagtcccc tcaacaatta ctaaatcaaa tgtaggattt
    11581 accactttat ctttgataat caacctccct cttctgttga gacaatgatc tcgcaatccg
    11641 atgcatactc ctatataaac tgccagcaat ccccacatat tgagtagatc atgtcagaat
    11701 gttctatgtc gtcagcaacg atacactaaa agtcacgttc accatgactg attgctattc
    11761 ccagcacaat tatttctata cacgtttcat gatttgaata aacactcaca tcatacatat
    11821 taccattcat acaccatact gcagccccta ctatatggtt gtaagctccc aaatctatat
    11881 ttgacttgat cactttggta gtagatctac taattctaaa tccattgctg ataaaatttc
    11941 cattttttcc tccttctttc tgccaaaaca gttaaaacaa taataatctt tatgtaaaaa
    12001 agggctgtga caaaagcctt gtcacagtcc catattccga ataaacggtg gtcaaaagct
    12061 tgcatctgcg gtaataagtt caaccaaacg aaaaataagc caacatttca caaaaattta
    12121 agaagcaatt ttcgtaaatt tcttcttatt tcttgaagct aaccacttct gttacaacca
    12181 cttttagaac atttaaagct tcgatgtgac aaaccatcta tgctgctcga tcataaaatt
    12241 gatagcaaaa attccacaaa gtacatatgt cccccatgaa aacaaaacat tcatatgcgg
    12301 tacaatgatc tttggaagaa tcacgatcaa aaatgatgcc agtgcccaaa tccatcgatt
    12361 gatttgaata tgatatgtag ctagcatatt caatataagg tgactagccg ttaataaaaa
    12421 gataaaaaca aaaaataaaa tcagccaata cattgtttcc caccttctaa aaaccaaact
    12481 tagacagtct ggacttcgtt ttctccacga ctttgttcat ctgacagcat ctttttatca
    12541 taagctttta caaatggata atagataata aatgctatca atgtgttgat catgatcaag
    12601 ataacaacac gccagtcatt tcctgctgaa agataaccgc cgattggagc tggtaatgtc
    12661 catgttggca aaacagtaaa tctattgacc atttctaatt tgactgcaaa ataactgata
    12721 atagtcaaaa ctaaaggaac taagttgaac gggatgaaca tgtaaggatt caacatcata
    12781 ggagctccaa aaattatggg ctcattgata ttgaagatac caggaataat agaaaaacga
    12841 ccaacctctt tcaagaattt tgatttacag aagaatataa acaaaacaga taccacgatc
    12901 gttgctcctg caccaccaat tgttaccgtc cattgataaa actgttcagg agcgatatag
    12961 ggtaattggg tggcagctgt accatctgcc aatgcctttg cattctcatc caacatgatc
    13021 agccacattg gacgaacaac agcccctaca atactcatgc catggatacc aaatgaccac
    13081 aacaaatgga tcaaaaacat tggtagtaaa acaccaagca aattattccc taaaatattg
    13141 gttaaaggct cgaataacga taataaggca gcgtttacat caaagcccgc gacgcttcga
    13201 atcagccaag taattgttaa aatgactgca cctggaatca atgcctcaaa actacgggca
    13261 acagcaggtg gcacttgttc tggcattttg atagtaatat ttcgattttt aaagaaacga
    13321 tatacttcca ctgcaaaaat catagataag atccccgcaa acatcccaga tgctcccaaa
    13381 gaactcatag gtaagaccca gccaagaggt gcctcttcag gagttaaaac gtttacagga
    13441 acttgtaaca taaagaacgt tcctaaagat aatataccac ctgtcacact atctaatcca
    13501 tatgatttag ctagatccga acccataaca aaagttgcat aaatagccat caatcccatc
    13561 gtcatcctaa agggtaaaac aatcgttgcc gaataagggg caataaattc attccatcca
    13621 ggaaacggta aattgataaa gaccataaaa aatgtcccaa tcaaaatcaa tgacaatgtt
    13681 gaaacaaccc cattacgaat tgctaataaa tgacgctgtg taccaatttt tgccattggt
    13741 gggacaagtt tctcttcgac ccatttcatc agcttttcca ctaaaaccac tccttttcaa
    13801 taaaatatga accattcatt gcatgacaac gcaaagaatg ttcataacta attatataat
    13861 taagaaattt tatttcaata tactctctta aataatcaaa tttattttta agaatcctga
    13921 aaataagttt tctaaatcat aggagcaaca aaaaaattcg ataattaaca aaaatcaaaa
    13981 tgattaaatc tagtgattga aacatatcat ttcaaaaaaa ttttgtaaat tcacccataa
    14041 atatcaccct atttaacttt tttcatcgta cactaaaatt caaaacaact atctttaatc
    14101 tttacttctt ggttttgttt taataaattt ccttcaaaaa tgcctggaga aactacaatg
    14161 atcaccgtat ctttttgtgc ttgctccaac ttcttcaaat ctataatagc taacagtgtc
    14221 tcttctttta cctcctggtt actttcaacc cgtatctgaa acggacttcc ttcgagttca
    14281 actgtatcta aacccatatg gatcagtatt tttctgccag attcactttc gatcgttatg
    14341 gcatgtcggg taggaaatat attatcgatt attcctttaa ctggcgcatg gatatatcca
    14401 tcatgattcg ttatagcgta tccaactcct atcatttttg aagaaaagac ctcgtctttt
    14461 actgctgtta atggaataat ttgaccacta gctacagctt ttagaatgtt tcttgtttta
    14521 aagaaatgca ctttttatca cctctacttt aatgctggcc aatatttttt gtttactttg
    14581 atcatttcat ctagtatttt ttttgcgact ctggcatttg gtacggtttg attcaatgta
    14641 aatgcttgta aagctttttg ataagaacct tcaaagtaag catctactag taacttctct
    14701 actgcaactt gtgcctccat caatccttta tgaaaatcac tgattggttt tcttagtgta
    14761 attggctcca caccagttgc accaacatat gccggtattt caacaactgc atctgaccgt
    14821 agattcggaa tcgccccttg attaggaacg attagcataa agcgacgacg atcatcattc
    14881 aacaaggaaa tagctatatc gacgatatat tgtccatgct ccccaaaatt aaagttcaat
    14941 acttcttcca caataccgcg gcgtatttta tcagccattt ctttggtatt tttctctcgt
    15001 ccatccatga ccatattagc acgtgtatac tctttgtcag tatgctcaac gaccatatca
    15061 ggatataagt aatactctaa gtaattattc gggatattac ttggaaaatt tttcgtgatg
    15121 actgacatca tttgaaaggt tttttgccaa gttttatctc ctatattaaa atcggcgact
    15181 tgcatttctt ttgttattag tttttcaata atctctggca taatttctcg ttgcaattct
    15241 ttatcataaa tcgatgtata ccagccaaag tggtttaaac cataatacgt aacgatccaa
    15301 ttcttacgat cataaccgta attcactgca atcgtttctt caattgaaat agtcatatca
    15361 caggcattaa tcatttttac attcggatat ttacgacgaa cagcttcaga aacgatcgac
    15421 tctggatttg tataattcaa aatccatgct ttgggggcat agtcttgtat atgatcgaca
    15481 atttccaata aacctttgat tgagcgcatc ccataagcaa atcctccgag tccacacgtt
    15541 tcttgtccta ctaatccatg tttaagtgga atcttttcgt ccttttctcg catctccaat
    15601 cctccaacac gaatttgtga gaaaacaaaa tcgcatcctg taaaagcaag ctttggatca
    15661 tccgttgcac gaatgcgaat atttgaaaaa ccttttcttt tcagcataaa tttaacaatc
    15721 aagtacatat ccatatttct ttcttcatca atatcatata aacgtatttc agataatggc
    15781 aaccgttttt cattgttcaa aatggcttga acgattcctg gtgtatacgt actcccgcct
    15841 ccagctatag tgataatctg ttttttcatc atcatctttt cctttctatt cttctaataa
    15901 tttattgaat tctgccataa cataagtcac ttctaagccg atgataattt gataattttt
    15961 ttcagttggt ttaatgatcc cgcttatccc taattgcttg attcgctgtt cctcgaccat
    16021 ggtgggatcc actacctcta cccgtaggcg tgtattacaa ctatagacat ccaggatatt
    16081 ggcttttcct ccaaacgctt gtagcatttt tgttgccata tattcataat ttttacttgc
    16141 taaccctaac tctgtttctt ctaaggttac ttcattgcca aactctgcct tagcttcacg
    16201 tcctaatgtc tgaatgtttc tcttattgat caaataataa aaagtaacgt aatacaaggc
    16261 aaaaaagaaa attccgattg gaagaatcaa aatagcattg gtagcgattc gaaaattgat
    16321 gagataatca acaatacatg ctccgaatgt aaaaccaatt cgtatattga ataagtaaca
    16381 gacaatccct gctaaaccgg caaaaagtgc atggatcacg tacaacatag gagaaagaaa
    16441 catgaaacta aattcaacag gctcagtgat atttgcaata aatgaagttc ctgcaccgct
    16501 tcccatgatt cctttgacca tctctttatt ttctttataa gctgctttat agatagctag
    16561 acatatagct ggtataccaa acatcattac tacaaaaaaa cctgataaaa acagtccagc
    16621 ctgaggatct ccgttgataa agcgtgtcat ctctcctctg aaaacttcct tgctaggaga
    16681 tgtatagctt cctaaatcgt aataaatata cgtattcaac acatggtgca gtccaaaggg
    16741 gatcagcaaa cgattcaaaa acataaatat ccccacacct aatgctccca tatcaacgag
    16801 cagttttgca aaattatcaa tcccaatttg gatgagcggc cagataaagc ccaaaaaaac
    16861 agctgcgatt gtttgtagaa tcatcaccat agtcaatggg aatttttcgc ccgcaaaata
    16921 agaaaaaact actggtaatt tttgttcttt aaagcgatta tacgtccatg ccgctactaa
    16981 accggaaata attccaccaa aaatccccat attcacttct gggtccataa tttctaagcc
    17041 ttttttcaag gtagcaatcg ctaaaaaacc agtaagtgca ggtattcctt tgtccttatt
    17101 tttgacaaaa ccgattgcaa ctcctatagc aaataagata tccaagtttc caaaaacggc
    17161 atttccagct gcaccaatta ctgggatgtt cagcatatcg tcagctgata aacgcatgat
    17221 caatcccgct aatggcatcg ctgcaactgg aagtagcatc gctcttccaa gcttctgcac
    17281 atttgattta acattcattt ttattcctcc tctattaaat atcctcatat gttactaact
    17341 caatcaaagg atcatttttc actttttgag caaatgctga atgaattaaa aattcgactt
    17401 ggtgggtacg aggatacaca aaacttgaac catttaacaa ttcctgatcc aaatacgctg
    17461 cgtgacacat gacttcgatc gttttatctt ctctcaatat catgagtaaa cttgccaaat
    17521 attcttccac actcgtattg atttgtcgtt ttttttcatc agtttctcct acgacatcga
    17581 aacaaggctc aaaataatcg acatgacgaa cttcatcttt tcgttcaaag ttgctccgaa
    17641 ccggcaaatc atattttttt gctaatgtaa caactacctc ctgattttca tccaaagtat
    17701 gagtatgatg atgactgtct aaatgagttg gtatgatccc cgcatgataa attttttgaa
    17761 tctgagcatt ccattcctga tacagttgat ctttgtcaat gttaaatgga tgctgataat
    17821 aagataagga tctgaactct ccgctttcga taagagtatc gacagttttg aggagtggtt
    17881 tgccacacgt caatgttaaa tggactccta cacctagcct aggcatttcc tttcttagtt
    17941 tcacagcatg ctcgaaaccc ggcatatttg ccatcaatgt agttgatgtc aaaattcctc
    18001 gctgataagc atccataatc ccataattca caccatggct atagccaaaa tcatccgaat
    18061 taaaaatcac ttttcccatt tcattctcct tcttttttct actaaaatag gcgcaaccaa
    18121 ataatcatag tccatttact atgtgtcatt tggttgcgcc tatagatagt tacgccctta
    18181 ttttaataga ttcttgctag atgaattgcc aaatagatga tttcattttc ttgaatggca
    18241 agttgaaatt gctgttcgat gatatcttta atctttagtg ctgtacaata cgctttgcta
    18301 tgtttaaatg tttcagtaaa aaaaccaata ttttctactg gcgtttcttc ctgataaatc
    18361 aagcgtttaa ccaggaattt gatatgaacc attaagcgat gataagtcag tgattcttta
    18421 tcataaacgt ttgaatagga tttttctaga tgggacaaaa tcgtggtcgt catttctact
    18481 atttttaagc tctttaaact attaggctct cctagctcag cattaatgat atgcatagtg
    18541 atgaatccta cttcatttga ccctaactcg gtttgatatc gatgattgat tcgctcgacc
    18601 acttctactg ctatatcata ttcatttgga taaaggaatc tcatttctgg caacataatt
    18661 gctgtgacat ctctatcttc tctttttcgt tgaatagcaa aataaatatg atctgtcaat
    18721 gtaatataga tatttgaatc taatggagag gagatcctag ctgttatcag ttcaatcact
    18781 tcttccgcaa aatccataaa ttcgattgga atctgcgaga ctatctcact gaatcgtttg
    18841 atcttttctt tagaatctaa tacaaataat ttgtcagcat cctcaacatc aaatggctta
    18901 ctcactttag cagaaaagcc aatccctttc cccatcgcaa ttttttctat tccatcgtca
    18961 tcgatcaata cagcattatg attcagcttt ttcatcacat acaccgttaa ttcacctcct
    19021 ctatatgata aaacaaaaca acttgcagac tccttcatac atatttttgt ctccaaacaa
    19081 tttttaaaga caaaaaacga cctaagaaag aaccctttct taggtcgtgc ctaattgaat
    19141 agtagcaccc tacataattt acaaaaatag tttaacacat aataaaagcg ttatcaatat
    19201 cttttttact cgttttatga taatacttgt atcgaattga tttggaggta attacataaa
    19261 aacaaaaaaa atatagcaac tctctataag aaagttgcta tattgataat tataccggcg
    19321 gccggggtcg aaccggcacg tcctcaacgg acactggatt ttgagtccag cgcgtctgcc
    19381 aattccgcca cgccggcaaa tttaaaaata ataactgggg tagctggatt cgaaccaacg
    19441 catgagggag tcaaagtccc ttgccttacc gcttggctat accccaagaa taaagggcga
    19501 atgatgggaa tcgaacccac gaatgccgga gccacaatcc ggtgcgttaa ccacttcgcc
    19561 acattcgcca tcataaaaga tgttatttaa tttctcaata aaaaactaag atgaagacat
    19621 cttagtcaat accggcggcc ggggtcgaac cggcacgccc tcaacgggca ctggattttg
    19681 agtccagcgc gtctgccaat tccgccacgc cggcaagttg atttattagt actaaggcgg
    19741 taaccggatt tgaaccggtg atgaaggttt tgcagacctc tgccttacca cttggctata
    19801 ccgccattat actattatca atctataaaa taaagaataa attatagaaa ctggggtagc
    19861 tggattcgaa ccaacgcatg agggagtcaa agtcccttgc cttaccgctt ggctataccc
    19921 caagaataaa aagggcgaat gatgggaatc gaacccacga atgccggagc cacaatccgg
    19981 tgcgttaacc acttcgccac attcgccatg gcaggggcag caggaattga acccacacta
    20041 acggttttgg agaccgctgt tctaccttta aactatgccc ctataaaatg gaggagagtg
    20101 gattcgaacc actgaaccct aaggaacgga tttacagtcc gtcgcgttta gccacttcgc
    20161 tactcctcca tggtggcgcg ggacagaatc gaactgccga cacacggagc ttcaatccgt
    20221 tgctctacca actgagctac cgcgccatat tttaaaatta ttaattgaat aacaagaacg
    20281 acggttccga cgggaatcga acccgcgatc tcctgcgtga caggcaggca tgttaacccc
    20341 tacacctcgg aaccagttca ttatggaggt taacgggatc gaaccgctga ccctctgctt
    20401 gtaaggcaga tgctctccca gctgagctaa acctccaaca ttcaattaaa atgacccgta
    20461 cgggattcga acccgtgtta ccgccgtgaa agggcggtgt cttaaccgct tgaccaacgg
    20521 gccaggtaaa tttgctacgg agagtaaggg attcgaaccc ttgagacagt ttggcaccat
    20581 ctacatgatt tccaatcatg ctccttcggc ctctcggaca actctccaga gtgaaagaag
    20641 ctcaatactc tttctcaaaa tgagaactcc ggcagtagga ctcgaaccta cgacatcatg
    20701 attaacagtc atgcgctact accaactgag ctatgccgga ataacatcag cgtggcgacg
    20761 tcctactctc acaaggggca acccctcact acaatcggcg ctaagaagct taacttctgt
    20821 gttcggcatg gttacaggtg tatccttctc gctatcgcca ccacactgtg gtgttatctt
    20881 ttattgagta aattttgttc actcaaaact ggatttgaag tcacataagt ttttttccga
    20941 gt
//
LOCUS       DACCKU010000051        20358 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.053, whole genome shotgun sequence.
ACCESSION   DACCKU010000051 DACCKU010000000
VERSION     DACCKU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.053"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            292..1431
                     /locus_tag="IVN13_001738"
     CDS             292..1431
                     /locus_tag="IVN13_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP7012215.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLARHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHSIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1498..2073)
                     /locus_tag="IVN13_001739"
     CDS             complement(1498..2073)
                     /locus_tag="IVN13_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7012216.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQVGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(2078..3052)
                     /locus_tag="IVN13_001740"
     CDS             complement(2078..3052)
                     /locus_tag="IVN13_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP7012217.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPVDIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGLDGLGDTNFPMVLAKQAESL
                     HAVDFLTDYFKEKTDTSVIALGPLTNIASALKNSPSVGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFEKLKQPIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIDESICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(3076..3120)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            3335..4303
                     /locus_tag="IVN13_001741"
     CDS             3335..4303
                     /locus_tag="IVN13_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7012218.1"
                     /translation="MVIFYFILILIFIGISYHFLSYFYAKKLVRAGVQRGYDWYEETG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGIPKTIKSYDGLKLAGQMFLQPTQQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLIPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPDASIILHGSSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMIMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            4416..4694
                     /locus_tag="IVN13_001742"
     CDS             4416..4694
                     /locus_tag="IVN13_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7012219.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARREGKKIIPSCPYAKKEFEKKPEYHDVLRQS"
     gene            4812..5048
                     /gene="secG"
                     /locus_tag="IVN13_001743"
     CDS             4812..5048
                     /gene="secG"
                     /locus_tag="IVN13_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP7012220.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAVWMILLFVLAFLSSK"
     gene            5160..5906
                     /locus_tag="IVN13_001744"
     CDS             5160..5906
                     /locus_tag="IVN13_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7012221.1"
                     /translation="MKQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVETNVPENFEKYVRQVLKIAGKSEKEINEKAVSYRSLAEH
                     QLMDIQDQAAIAESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            5909..8266
                     /gene="rnr"
                     /locus_tag="IVN13_001745"
     CDS             5909..8266
                     /gene="rnr"
                     /locus_tag="IVN13_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP7012222.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILVEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSDEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDIVGRRDLRDQLIVTIDGEDA
                     KDLDDAVTVKKLENGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHEIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILEMMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKSGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            8288..8752
                     /gene="smpB"
                     /locus_tag="IVN13_001746"
     CDS             8288..8752
                     /gene="smpB"
                     /locus_tag="IVN13_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP7012223.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            9054..9773
                     /gene="atpB"
                     /locus_tag="IVN13_001747"
     CDS             9054..9773
                     /gene="atpB"
                     /locus_tag="IVN13_001747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP7012224.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            9826..10041
                     /gene="atpE"
                     /locus_tag="IVN13_001748"
     CDS             9826..10041
                     /gene="atpE"
                     /locus_tag="IVN13_001748"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP7012225.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            10132..10656
                     /gene="atpF"
                     /locus_tag="IVN13_001749"
     CDS             10132..10656
                     /gene="atpF"
                     /locus_tag="IVN13_001749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP7012226.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            10643..11185
                     /locus_tag="IVN13_001750"
     CDS             10643..11185
                     /locus_tag="IVN13_001750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP7012227.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            11208..12764
                     /locus_tag="IVN13_001751"
     CDS             11208..12764
                     /locus_tag="IVN13_001751"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP7012228.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEETLNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            12778..13680
                     /locus_tag="IVN13_001752"
     CDS             12778..13680
                     /locus_tag="IVN13_001752"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP7012229.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            13703..15109
                     /gene="atpD"
                     /locus_tag="IVN13_001753"
     CDS             13703..15109
                     /gene="atpD"
                     /locus_tag="IVN13_001753"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP7012230.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            15123..15545
                     /locus_tag="IVN13_001754"
     CDS             15123..15545
                     /locus_tag="IVN13_001754"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP7012231.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            16014..16244
                     /locus_tag="IVN13_001755"
     CDS             16014..16244
                     /locus_tag="IVN13_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP7012232.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            16341..17642
                     /gene="murA"
                     /locus_tag="IVN13_001756"
     CDS             16341..17642
                     /gene="murA"
                     /locus_tag="IVN13_001756"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7012233.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNCPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            17655..17831
                     /locus_tag="IVN13_001757"
     CDS             17655..17831
                     /locus_tag="IVN13_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP7012234.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            17966..18262
                     /gene="yidD"
                     /locus_tag="IVN13_001758"
     CDS             17966..18262
                     /gene="yidD"
                     /locus_tag="IVN13_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP7012235.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            18326..18709
                     /locus_tag="IVN13_001759"
     CDS             18326..18709
                     /locus_tag="IVN13_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012236.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHH"
     gene            18878..19762
                     /locus_tag="IVN13_001760"
     CDS             18878..19762
                     /locus_tag="IVN13_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7012237.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            19879..20112
                     /locus_tag="IVN13_001761"
     CDS             19879..20112
                     /locus_tag="IVN13_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP7012238.1"
                     /translation="MSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            20200..20280
                     /locus_tag="IVN13_001762"
     tRNA            20200..20280
                     /locus_tag="IVN13_001762"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20234..20236,aa:Tyr,seq:gta)
     gene            20287..20358
                     /locus_tag="IVN13_001763"
     tRNA            20287..20358
                     /locus_tag="IVN13_001763"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20319..20321,aa:Gln,seq:ttg)
BASE COUNT         6758 a         3566 c         4425 g         5609 t
ORIGIN      
        1 tttttttgca actagcttct gtatatgatg tttacaaaga atggtacaag aaaagatcat
       61 ccttgaaaaa ggaaagctaa caataactag catcatataa gagcttttaa ggatagaaca
      121 gtagaatagg aatctatggg caagtcctct cgtataaata aaataggagg gaagtagcag
      181 catatttttc ataaagtggc tctctttctt catttttact ttggttgctc ctatttgaaa
      241 attcgagcac actcaaatac aataaaaaga aatggcaagg gagaggatta gatgaaaaaa
      301 gaagaaaaaa tcgcgatttt acaagagatt atccgaatta aatcagtgaa tggaaacgaa
      361 ggtgaagtag cagcctattt gaataagctt ttggcaaggc atgacataac aggagagatt
      421 gtttcttaca gagatggtag agataatttg attgctcgtt accaaaaggg ccaatcagga
      481 aaagtgttag gcctatctgg acatatggat gtcgtggcgg ctggtgatga atcgtcttgg
      541 acctatgcac cctttgcagc agaaatccat gggaaccgtt tgtacggacg tggtgctaca
      601 gatatgaaga gtggcttggc agcaatggtg attgccatga tcgaactgaa agagtcagga
      661 aaaccattta acggaacagt caaattatta gccactgtcg gagaagaagt gggtgaacta
      721 ggtggggaac agctcacgaa agcaggctat gtagatgact tagatgcctt gatcatcgga
      781 gagccaacca attacagttt gatgtatacg cacatgggat caatcaacta tacagtcacc
      841 tcgcacggaa aagaagcgca tagctctatg ccagaccaag gttataatgc aatcaatcac
      901 ctaaatgaat tcattacgaa agcaaatgct gaaatgaatc acctcgctga aacaatcgag
      961 aatcctgtcc taggaaaaac aatccataat gttacattga tcagtggcgg gaatcaagtc
     1021 aacagcatcc caagccatgc gcagctccaa gggaacattc gatcgattcc agaataccca
     1081 aatgataaaa tcattgcatt gctgcaatca atcgtcaatg aattgaatca agagacagac
     1141 tatcatttgg aattgatgat cgattacaac aaaatacctg tcaaagctga tccagattct
     1201 cctttgattc attccattca gcaacaattc agccagccat tacctttagt tggagcggca
     1261 gcaacaacgg atgcagccga atttacgaaa gctaaccatt ccttcgactt tgtcgtcttt
     1321 gggcctggtg tcgttacttt gcctcatcaa gtagatgagt atgttgaaat cgataattat
     1381 ttggatatga tcgaaaaata tcaaggaatt attttatcct acttagcgta agattttttt
     1441 aataaatgaa aaaacagagc cagattagca ttattttgct aatccagctc tgtttattca
     1501 gcctattttt ctttttgaat atatcaagcg ttggggcaac atagtgatga ctaatgaaac
     1561 aacgacgata gacaacaagc gatcaaggta gtctgtaccc acctgtacca agatcacact
     1621 tgttgtctga cttaaaccta acccatgtaa tagctgaacg atcatactgg atcctgagga
     1681 ggtgatcccg ccaaataact gaacggtaat gatcgaagaa acgatcgttc caggtaccga
     1741 caaaatcaaa gcgagtaata acagtttcaa gctgttttta tctttgccga gatatcttgg
     1801 caaaatcaat ccagcaagca agcctgtgag aatttggatt ggcgaataat acagagaaaa
     1861 aatatctgta gtcactccac ttaaaatacc gctggacaat cctgtcagca ttccaggtac
     1921 aggcccaagc aatgcaccag caaaaatcgt tccgatagaa tcgagataga ttggcaaacg
     1981 aagccataaa gcaatcgttc ctccaatata attgatccca atacacaaag ccatcactgt
     2041 aagtacgcga gttgccattt tgtttttatt cattttgtta gcccatcttt aacctttcca
     2101 aatctttcat aatcgtttct tcttgtgcat ttaaaagaac ggttaaaaac tttcggaaaa
     2161 aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc caaaattcat
     2221 aacgatcaac caatgtttgt ccgcgactta ttccttgagt ttctacagca gtgaagcttt
     2281 taaagccttc acagatggac tcatcgataa aataagcaac agctaatgga tcattgatga
     2341 cacatcctaa gattctttct tgtttccagt gaaagtcaaa ataaaaacga gtgatcgctt
     2401 ttagatactc tccctcttca ggattcattt ggcagcaata ttcaaggata gtcggtgtca
     2461 agacgatttc tcttgtcaca tctaacccaa ccatttctat cggttgtttg agcttttcga
     2521 atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact ggtgaacagt
     2581 taccatggct tttgtaagtt ccgcccattg agacaaaacg atccatatgt ttcccaacag
     2641 atgggctgtt tttcaaagca gaagcgatat tggtcaaagg ccccaaagca atgactgatg
     2701 tatctgtttt ttctttgaaa taatcagtca gaaaatcaac tgcatggagc gattctgcct
     2761 gtttggctaa gaccatcggg aaatttgtgt cccctaatcc atccaaccca tgagtgtctt
     2821 gcgcactaat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca gggatatcta
     2881 aacgatcaca gcgctccaaa catttcagca cattctgcgt accgatatct acagggacat
     2941 tcccgcaaac gacggtaata gcaactactt cgatatcagg ggatttgaca gctagtgata
     3001 aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tactttacgc attatttcca
     3061 atctcctttc tttagagata cttagttttt tattctggga agtttccgaa ccagtccaag
     3121 acgagtgtct tgtttataat gcacgaaatt agtatactgg cttttattgt gaatgtaaat
     3181 gctctcttac aagtaaatct attgagaaga actttaagta aatgatacaa tgaaaaagaa
     3241 atggaattta cgtgtgccga agaaaaagaa tcctattgat cggtatattg tataataaat
     3301 agattatagt ttggcagaaa gcggcgggtg tacgatggtt attttttatt tcattcttat
     3361 tctgatattt ataggaatca gttaccattt tctttcttat ttttatgcaa agaaattagt
     3421 aagagcgggg gtacagagag gctatgactg gtatgaagaa actgggcacc gcttgatgga
     3481 tcctgctgca gtcacgtcag tagaaaaaaa gcgacaaaaa ttagaagaag agtgtgaaag
     3541 cttctgggaa caaggaatac cgaaaacgat caaaagttat gacgggttga aactagctgg
     3601 acagatgttc ctccaaccaa ctcagcagaa taaatgggtc atttgtgtac atgattatcg
     3661 aagtacaggg aaaagagata tgtctcatat aggtaaaaga tatgcagaga aaggattcaa
     3721 cgttcttata cccgatttac gtgcgcatgg agaaagtgaa ggagaaatca tcggaatggg
     3781 atggctagac cggctagatt taattgcatg gatccaactc atcttagatg aacaaccaga
     3841 tgcatccatt attttgcacg gcagttcgat gggcgcttct actattatga tggcaagtgg
     3901 tgaaaagctt ccatctgcag tcaaaggatt tatcttagac agcggatatg tttctgttta
     3961 tgcggaattt cggtatatgt taagtaaaat cacggtcttt cctaaaaaaa tgatcatgag
     4021 atatgcgaat cactatgctc agaaatacgc aggttattct ttaaagcaag catcagctac
     4081 aagacagctt ggtagtaacc atttgccttt gttagtcatc catggtgaaa gagatcattt
     4141 tgttcctaca gaagcagcat acacgattca aaacgctacg gcaggggata aagcattact
     4201 gcttgttcca gaagcagaac atttggaagc atcgatgaaa gatccgaaaa cctattggac
     4261 agtgattttt tcatttattg aacaaagaat aacgatggat tgattttctc ttcctttttg
     4321 taagcaacac gaggataaat aattgtttat agaccatgag actgttaaaa taaggtagga
     4381 ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagag gaaaaaaatc
     4441 gtcttgcttt attcaatgat gagcaacaag aaatcggaga aatgacatgg tcggatgcgg
     4501 ggccagacat catgatcatt gaccatacat ttgtcgatcc agcatatcgt ggtcaaaaat
     4561 tagctgaaaa gctagtgtat actggagtag aactagcaag acgagagggg aaaaagatta
     4621 tcccttcatg cccttacgca aaaaaagagt ttgagaagaa accagagtat catgacgtct
     4681 tacgccaatc ataataataa cagctgaaag atttgcactt tcagctgttt ttccatttat
     4741 atagagttgt cacggagttt tatctatgat aaagttaaag agacgatatc ttttgggaag
     4801 gaggctgcta tatgtataat ttaattttgg gaatcgtgat cgttttatcg attgttatgg
     4861 tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca tttaccggcg
     4921 gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc atgcagcgat
     4981 caactgcagt catgggagct gtctggatga tcttattatt tgtcctagca tttttatctt
     5041 cgaagtagtc gaataagcct ctcgcacaac ggcgggcggc tttttttatt gcaaagagaa
     5101 cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag gtgaaacgag
     5161 tgaaacaatt accaaaaccc ctttatgcaa aacatggaaa acgagcagtt ttgctgcttc
     5221 atgcgtactc aggaagtcca aatgatgtcc ggatgctcgc acggttttta gaaaaagcag
     5281 attatacagt ctatgcgccg ctatttaaag gacatggaac gatggagcca tatgatatct
     5341 tgcaagaaaa agcagaaagc tggtgggcag atactaaaaa agctatccat tttttacagt
     5401 ctgaacagtt ttctgatatt gccgtattgg gattatcgat gggtggcatc tttgctgtac
     5461 gggcactaga agaagaatca gtaattggcg gcggtttttt ctgttcgccc ctgtctcctg
     5521 tagaaacaaa cgtgcctgaa aattttgaaa aatatgtacg tcaagtgctg aagatagcag
     5581 gcaaatcaga aaaagaaatc aatgaaaaag ctgtgtctta ccgctcgtta gctgaacatc
     5641 aattaatgga tatacaagac caagcagcaa tcgcagaaag tagactatca gatatccaac
     5701 agccaatatt tttagctcaa gctgggaaag atgaaatgat cgacccaaat ggtgtatttg
     5761 aaacggcacg caaattgagc cgccaacgag tgacacttca atggtatcct gaaagcggtc
     5821 atgtgattac agttggcaca gcaagacgcg aattagaaaa agacgtgttg gaatttttag
     5881 aaaaattacc ttggaatgag gaataaatat gacaaaagaa acaataaaag agaaaattct
     5941 ctttttcatg gaaagcagtc gcaaacgtag tttttctatg gaagaaatcg ctgaaggtct
     6001 aggctttcaa aaaagcgatg attttaaatt actagtacaa acgattgctc aaatggaaag
     6061 agaacagagt atcgttttca ataaaaaagg aaaagtcaaa cttcctttaa aaccgatctt
     6121 ggtcgaaggg acatttcggg caaatgaacg aggatttgga tttgtaacga ttgatcccga
     6181 agaagatgat atctatatcc cgaaagaagc gactggcttt gcaatggatg gggataccgt
     6241 agcaattgat atcatgaagc gagcaaatac agatatggat cgcggagcag agggaaaagt
     6301 agtggagatt cgagaccgag cgactactca gttagccgga gaatttgttg cttattcaga
     6361 cgaagagata caggaaacgg atctttatgg tgtggtcatc ccgaaagata gaaagcttaa
     6421 ccaattcaaa gtgtatgcag cggcagaggg agttcgtcca gttgacggaa gtattgtatt
     6481 gatcgaactg acccattacc ctgaaaaagg gtatgcaact agtttagaag ggataatcaa
     6541 gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcgattg tcgtggctaa
     6601 cggtattcct acaaaatttc ctgatgatgt gttatctgag gcagatgaag ttccagacca
     6661 gatcagcgaa aaggatattg ttggacgcag agatttaaga gatcagctca tcgttacgat
     6721 tgatggagaa gatgcaaaag atctagatga tgcagtgacg gtcaaaaaat tagaaaatgg
     6781 taattatttt cttggagtac acattgcaga tgtatcttac tatgtgacgc aaggcagtca
     6841 actggacaga gaagcatacg aaagaggaac aagtgtctat ttgaccgatc gagtgattcc
     6901 gatgatccca caacgtttat ccaatggtat ctgttcattg aatccccatg ttccacgact
     6961 caccatgagt tgtgaaatgg agatcgatcc aaatggacaa gttgtctctc atgagatttt
     7021 tccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc aaatcttaga
     7081 agatcaagat gaagaaacga tgcaacgtta tgctcagctc gtgcctatgt tccaagaaat
     7141 gggtgaattg catcgaatcc ttgaaatgat gcgaatcaaa cgtggagcga tttcttttga
     7201 ggatcgtgaa gcgaagatcc ttgttgatgc tgaaggacac ccaaatgata tccagctaag
     7261 aagcagaggg ataggtgaac gcttgatcga atcctttatg cttgctgcca acgaaacggt
     7321 agctgaacat tataataaat taaatcttcc gtttatttat cgaatccatg aacagccaaa
     7381 agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc tggtccgagg
     7441 aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag tagaagacaa
     7501 accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag cacgctattc
     7561 agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact ttacatctcc
     7621 tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca gtgaagataa
     7681 aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag atcatagttc
     7741 taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga agaaagcaga
     7801 attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg ttgtgaaatt
     7861 cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca atgaattgaa
     7921 acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa gaactggtct
     7981 gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc cagatgaacg
     8041 agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac gtccaaaagc
     8101 acgcaatgga aaatctggaa aacgagaagg aaaacaagca ggcaaacgct cagaaaaaaa
     8161 tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga aaaaagggaa
     8221 aaagccattt tacaaaggtg tcaaaaagaa gaaaaagaaa aaataaaatg agttggagga
     8281 aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag ctcgccatga
     8341 ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg agatcaagtc
     8401 gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca acggggaagc
     8461 tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta atcatgatcc
     8521 gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg aaaacgaatt
     8581 gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg gctatgccaa
     8641 ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag atttgaaacg
     8701 aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat aatttcatta
     8761 tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg ttagcattta
     8821 atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg tgcatgggga
     8881 aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga aaatgttttg
     8941 aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg aaaaaaccag
     9001 atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat ggattggatg
     9061 aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt tgtataatgg
     9121 tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga aatcttaagt
     9181 tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt cgaggaatcg
     9241 taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca tttacattgt
     9301 ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc ttacctaacg
     9361 aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact ttggcattta
     9421 taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa ggatatttcg
     9481 taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt gaagaattta
     9541 caaacttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg gaagttttgc
     9601 taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca ttagcaattc
     9661 cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa gcatttgtct
     9721 ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa taatactcgg
     9781 ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa ttatatcgca
     9841 gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg tcaagttatc
     9901 tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt ccgtacaaca
     9961 atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt tatcgccttg
    10021 attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat cgtttgttac
    10081 tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc tatgctgaat
    10141 caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt tgtcagcggc
    10201 tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat cagcgatatt
    10261 ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca atctcgcatc
    10321 aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg ttctgatgca
    10381 gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa tattttgaag
    10441 gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat cactgtagaa
    10501 cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct tcaaatcgcg
    10561 gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat caatcaatac
    10621 attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa gacgttacgg
    10681 gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct atcaagatct
    10741 cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt taagtgatgt
    10801 tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg gttatgatgg
    10861 aatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg accttttgtt
    10921 catgattgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat tgggaagtgt
    10981 cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga atgttgcaaa
    11041 aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta ttattggtgg
    11101 cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc aattagaaaa
    11161 aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg gccatcaaag
    11221 cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac gtgctttcag
    11281 tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca catggtttag
    11341 aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga atggcgcaaa
    11401 acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc attcgtgaag
    11461 gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag gccttgatcg
    11521 gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc gttactgaca
    11581 aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct gttaacgaac
    11641 caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc ggacaacgtg
    11701 aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgattgat acgatcatca
    11761 accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa gattctacag
    11821 ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca atcgttgtga
    11881 atgccggtgc gtctcaacca gcaccattac tttatatcgc accatatgct ggtactgcaa
    11941 tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat gatctttcaa
    12001 aacaagctgt tgcttatcgt gaactttcct tgctacttcg ccgtccacca ggtcgtgaag
    12061 cttatcctgg ggatgttttc tacttgcact cacgtttatt agaacgtgca gcaaaattaa
    12121 gtgatgatct aggcggcggt tcaatgactg ctttgccatt tgttgaaaca caagctggag
    12181 atatctctgc ttatatccca acaaacgtta tctcaatcac tgacggacaa atcttcttag
    12241 aaagcgactt gttctacgcg ggtactcgtc cagcggttga tgccggacta tctgtctctc
    12301 gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact ttacgtctag
    12361 acttagcaag ttatcgtgaa ttagaagcat ttacacaatt cggttctgat ttggatgcgg
    12421 caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa caaaaactgc
    12481 atgcaccgct agctgttgag aaacaagtag tgattttgta tgccttgaca catggattct
    12541 tggacagtat ccctgtagac agtattttag acttcgaaca tgaattattc gaatatctgg
    12601 ataccaatca ttcagatatt tttgaaacaa tccgtacaac aaaagatctt ccagaagaag
    12661 aaacgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca aaaggaaata
    12721 attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt gagatgaatg
    12781 ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac gagccaaatc
    12841 acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc ttcttcacag
    12901 aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac tgctactcag
    12961 ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag catgctgctc
    13021 agccgtcctg tgaagaaaac cggttatatc gtcatcactg cagatggcgg tctggtcggt
    13081 ggatataaca gttcgatatt gaaacaaacg atgtctattt tagaagaaga ccaccaatca
    13141 cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt taaagcacgc
    13201 ggtatcaacc tagcatatga gctgcgcaat ttatctgatc agccaagctt tgatgaagtc
    13261 cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga tgaattatac
    13321 gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga aaaaatgctg
    13381 ccaatctcag acttagatcc agaagaagca acgactttcg aacaagaata tatttttgaa
    13441 ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag tttgatctac
    13501 ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc aatgaaaaca
    13561 gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttataa ccgtgcaaga
    13621 caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc actagaatag
    13681 tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa tcggtcccgt
    13741 tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg cattagttgt
    13801 ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag aattagggga
    13861 cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag gaatggaagt
    13921 cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag gtcgcgtatt
    13981 caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg cggaaagaag
    14041 cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag aaattttgga
    14101 aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga aagtcggact
    14161 attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc ataatatcgc
    14221 ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc gtgaaggtaa
    14281 tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca tggtgttcgg
    14341 acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt tgacgattgc
    14401 ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca acattttccg
    14461 tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt ctgcggttgg
    14521 ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca catctacgaa
    14581 aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact acactgaccc
    14641 ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac gtaaattaac
    14701 agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg ctttagcacc
    14761 tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg tattgcaacg
    14821 atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat ccgatcaaga
    14881 aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa actttaacgt
    14941 ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa cagtgaaagg
    15001 attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt tccgaagtgt
    15061 tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga aaggggtgcc
    15121 ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc tatgatcacc
    15181 atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg cctaaacatg
    15241 caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca gattcagata
    15301 cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat aatgttgttt
    15361 caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt gcggaacgtg
    15421 caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat acgaacgaat
    15481 tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta tctaagcact
    15541 cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag gttatgacag
    15601 aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca aatttttgtg
    15661 aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatca gaggttgtga
    15721 aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct catattttgc
    15781 cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg gaataaggga
    15841 ctgcgacaag acttttgtct cagtcccttt ttttgcatat ttttagcatt tatttcctaa
    15901 aaaatgtaag gggattccta tatttttaag tatacttgtt tttcctttta tacattttat
    15961 ggtattatca ttttattagt tattataaaa caatcgttag aaaggacgtc attatgcaag
    16021 tatacggaat tgatgcggtg cttcgaatcg tgagtcattt ggcgtttatc tacttagcct
    16081 tctggtcttt acgttcgtta cgcattgaaa cttttttcag accaatgcat aatacgcaaa
    16141 ttagaatggc aatcgtactt ttttcaattt ttttagggta cactgcaagt aattttttct
    16201 tagaagttat cgctttatgc cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta
    16261 ctattttaaa agcaaggatg ttataataga aaagattttg agtaagtgta tgctcattgg
    16321 aattttttgg agggaacaac atggaagaga tcatcgtaag aggtggcaat caattaaatg
    16381 gaacagtaag aattgaagga gctaaaaatg ctgttttgcc tattttagct gctagtttgt
    16441 tagcagaaga aggtattacg acattagaca acgttccgat tctttctgat gtcttcacta
    16501 tgaatcaagt gattcgtcac ttaaacgtag atgtagattt tgatgaacaa aagaatcaag
    16561 taacaatcga cgcttcaaga caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa
    16621 tgcgtgcttc aatcgttgtg atgggtccgc tacttgccag aaatggacat gcaaaagtag
    16681 ctatgcctgg tggatgtgcg attggtaaac gaccaatcga tttgcattta aaaggatttc
    16741 aagctttagg agcaaaaatt attcaaaaaa acggctatat cgaagcaatt gcggatgaat
    16801 tgatcggtaa cacgatttat ttagatttcc ctagtgtggg tgctactcaa aatatcatga
    16861 tggctgccgt aaaagcaaaa ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa
    16921 tcgttgattt agcaaacatt ttgaataaaa tgggcgctca agtctatgga gctggtactg
    16981 aaacaatgcg tattgaaggc gtggatcatt tacatgcagt caaccactca atcgtacaag
    17041 atcgtatcga agcaggtacc tttatggttg ctgctgcaat gacacaaggc aatgtgctga
    17101 ttgcagatgc tatctctgaa cataactgcc cattgatttc taaattgatc gaaatgggtg
    17161 cagaaatcat tgaagaagaa ggcggagtcc gtgtcatcgg accaaaacat atcttaccta
    17221 cagatgtaaa aacgatgcct catcctggtt tccctacaga tatgcaagct caaatgacag
    17281 ctatccaatt agttgcagaa ggtacaagtg tggtgactga aacagtgttt gaaaatcgtt
    17341 tccaacattt agaagagatg cgccgtatga acgctcatgt caaaatcgac ggaaatgtag
    17401 caatcatgga tggaaaccat gaattgcaag gtgcagaagt atatgcaaca gacttgcggg
    17461 cagcagcagc attagtttta gctggcttga aagcaaatgg aatcacacgt gtgcgtaact
    17521 tgaattatct agaccgtgga tattataatt tccatatcaa actacagcaa ttaggtgcag
    17581 acgtcgaacg tgtagacatg gatcaaactt ctgctgaaaa aacagcacaa acgattgctt
    17641 aaggaagtgg ctgaatgagt aaccaacgat atatcttatt aactttgata aagattttag
    17701 tggtgatctt attgcttatc cttctttttg tggccggaac gatgatcgga tatggtgtca
    17761 tcggtggcgg caatccattt aaagtattcc agccaagttt gtggattcat attcgagact
    17821 tttttcattg ataaataaga atcacagtgg ttctagtcga tatgaagtga gccatgaagc
    17881 agaacatgat aaaagaaaac agaagggaac gttgacgtta gtcgcgatcc ctttttttag
    17941 taaaagaaaa gaaggaatct aattgatgaa gaagatattg atttatttag ttagaggata
    18001 tcagcgcttt atttcgcctg catttccacc tagctgccgt tattacccaa cctgttccaa
    18061 ttatatgata aaagcaattc aaacccatgg tgcgctaaaa gggacggtaa tggggactgc
    18121 acggatctta cgctgtcatc cttttgtcaa aggcggtatc gattatgtac cattgacatt
    18181 ccgattaacg cgaaataaga aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa
    18241 tacgggtagc actgagcgat aaagaaaaag aggaaattta ttgtcaaagt aacaactccg
    18301 caactactta tatgggggac aaaaaatgat cgaagaacta aacaagaaag atttaccatg
    18361 gtccttgtta ttagatgcag atcctgataa agaaaaagtt caagtatatg tttccagagg
    18421 gagtggctgg atatggaaag aaaaagaaaa aacaataggt gtactgatct atgtggcaag
    18481 ggaaaaagaa tacgaaatcg tcaatgtagc agtaacccca acgtatcaag gaaaggggat
    18541 tggcggaaaa ttacttgaga cagcttttca gaagctttca cagctagctt caaaccaaac
    18601 tcggatcatt atacgtactg gcactactag tagcgctgcg ttacacctct atcaaaaaat
    18661 gggctatgtg gagattggtc gagtcaaaga ctattttatc catcattaat ctgagcccat
    18721 ttttgaaaat ggagatcaat taagggatca agtgatttta gcaattcact tacctaaaaa
    18781 tagctttaac taataaactt tcaattcgag aaaatgcttt caaatgctaa atagatacat
    18841 tacaatagct ttagcaaaca aaaaggagaa tatttttatg ggaatattag tttttgattt
    18901 tggtggatcg gctgtcaaat atggttgttg ggatggcaaa gagattaaag gtaagggaca
    18961 atttgcaacg cctgaatctt gggaagaaat gaaaacgcaa ttatttcaag tttataaaaa
    19021 aatactcgtt tcttttgaag tcgaaggaat tggaatcagc tctcctggag tagtgaataa
    19081 tgagaaacag gtgattgagg gaatcagtgc gattccttat attcatggat ttaatatttt
    19141 ccgagatttg gaaactctgt ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc
    19201 cggaatggct gaattttatg aaggtgctgc ccgtgattat caagatgttg cttttgttgt
    19261 ggcaggaact gggattggcg gggcgttgtt cacgaaagga caaataaata aaggggcaca
    19321 tctttacggt ggagaatttg ggttgatgtt cttagatggc gataagacat tcagtaaact
    19381 tggtacagcg gtacagatgg cttggcgtta ttgtgaacgc aaaggtctcg ataaaaatgc
    19441 ttataccgga aaagatgtct ttgaattagc agaaactgga gatgcaatag caaaagaaga
    19501 agttgagagt ttttatacgt atcttacaaa gggattattc agtattcaat tttcgtttga
    19561 tccagaagtg atcgtattag gcggcggggt ttctgcaaaa gaagggctca ttgatgagat
    19621 caaaagccga atgaagaagc tgacagaaca attcgatctg catgatttcg aaccgcagat
    19681 tttattatgt gagtacagaa acgatgctaa tcttgtaggt gcagcagcaa actatctcgc
    19741 tgtacgtcaa ggagagaatt aagagcttta gagaaggtgc agcttgcata aaagaagaga
    19801 atgaaatgaa taggagattt ttacctaagg aatgttttat ttgctgcagt atgataaaat
    19861 atctgagagg tgaaaatgat gtcaaaaaaa aataaagata ttgaagtgcg tatagaagaa
    19921 gtgaaaaaag atatcaaagg taagaattat gaagtcactc agttatttat agggaaaaaa
    19981 atgattggtg agatcttagc ctatgggcca aaagaatatg aaattttctt tggtgaagaa
    20041 gattttggaa aagaaaaaag tcttgaaaat gcgatagaaa cagtgattcg acactggaat
    20101 ctgcacgaat aaaaaaatat caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa
    20161 ttaggtatta taactaagtc aggttgatac aaacctttcg gaggagtagc gaagtggcta
    20221 aacgcgacgg actgtaaatc cgttccttag ggttcagtgg ttcgaatcca ctctcctcca
    20281 tttcattggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
    20341 cgaatccagc taccccag
//
LOCUS       DACCKU010000052        19693 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.055, whole genome shotgun sequence.
ACCESSION   DACCKU010000052 DACCKU010000000
VERSION     DACCKU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.055"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(18..710)
                     /locus_tag="IVN13_001764"
     CDS             complement(18..710)
                     /locus_tag="IVN13_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7012239.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="IVN13_001765"
     CDS             705..971
                     /locus_tag="IVN13_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7012240.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="IVN13_001766"
     CDS             complement(1147..1716)
                     /locus_tag="IVN13_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP7012241.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKELKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="IVN13_001767"
     CDS             complement(1790..3079)
                     /locus_tag="IVN13_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012242.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKVQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESENKSKLTKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="IVN13_001768"
     CDS             3433..4281
                     /locus_tag="IVN13_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP7012243.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="IVN13_001769"
     CDS             4426..5367
                     /locus_tag="IVN13_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP7012244.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFELLLDLYFSLWHLRKEKQPE
                     LLILGSRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5722..7524
                     /locus_tag="IVN13_001770"
     CDS             5722..7524
                     /locus_tag="IVN13_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HAP7012245.1"
                     /translation="MTVDYDYWYAPFLSLMAYFMMLTVFRKKDLLYEMIAVVTVLFVY
                     LAARQFFSIRLLSGNTRFQSLTTVLLICFLTFSAILPLYLPKVHQSLEETSEPIREYL
                     NDEGLYDTLHEYQLTGSARTGFSENDEQLGGPLYDNGEVVFTANQKGNGYWKIENKNH
                     YTGSGWIEDQDAPIQPIQEFPVDITSYQTVIEDKENITLTLKKQAFLPYPYGNTTLTD
                     FSLASGQTLYYLPESQRFIPVPNESTRSATLQTERLVFTREELKSSQLKADDLENQQT
                     NLQLPKSLPKRIEDLAVELTKNETTLIRKVEAVEQYLKTSGNFRYSKTDTGHTPKEHD
                     YVDYFLFDSKVGYCDNFSTSMVVLLRTLGIPARWTKGFTNGLRTGTDNQGNEEYSILN
                     SHAHSWPEVYFPGFGWLPFEPTPSFANPMGSQPVAETTETVQPAIEQPNESENMAETI
                     SSERSEVIPSEETTLTPAYKIVLFSLGVCLSVLLFFLLLFRKSLRWTSYLIYLHFFTS
                     TNTIAYQKLLNPLETLVPRKPAESLQEYSLRIEQEFKLDLRFIRLTTQYEAEIYGRQA
                     DPVKFDKKTYLEITHIIRKTVYKKILTKGSRFKK"
     gene            complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IVN13_001771"
     CDS             complement(7636..9807)
                     /gene="copB"
                     /locus_tag="IVN13_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP7012246.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9827..12013)
                     /locus_tag="IVN13_001772"
     CDS             complement(9827..12013)
                     /locus_tag="IVN13_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP7012247.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTIIGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IVN13_001773"
     CDS             complement(12013..12222)
                     /gene="copZ"
                     /locus_tag="IVN13_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP7012248.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINRLGYKAEVI"
     gene            complement(12235..12675)
                     /locus_tag="IVN13_001774"
     CDS             complement(12235..12675)
                     /locus_tag="IVN13_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP7012249.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12749..13288)
                     /locus_tag="IVN13_001775"
     CDS             complement(12749..13288)
                     /locus_tag="IVN13_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAP7012250.1"
                     /translation="MKIIVIGQSGSGKSTLARNIKEITGFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13780..15246)
                     /locus_tag="IVN13_001776"
     CDS             complement(13780..15246)
                     /locus_tag="IVN13_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7012251.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15557..17641)
                     /gene="cadA"
                     /locus_tag="IVN13_001777"
     CDS             complement(15557..17641)
                     /gene="cadA"
                     /locus_tag="IVN13_001777"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP7012252.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTAEGLMTVNPEDVLIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDPTGIDPKKFMQYVASAEQFSTHPIAQSVLEGYAGPLL
                     PTANIQEFAGEGILAEVDGKQVLVGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGY
                     LVIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDK
                     VQRLEEILQSNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQP
                     SKIAEAIHLAKKTLKIVKQNIVFAIGIKILVLSLGAFGFASMGDAVFADVGVTVLAVL
                     NAMRSLHVKNKR"
     gene            complement(17783..17971)
                     /locus_tag="IVN13_001778"
                     /pseudo
     CDS             complement(17783..17971)
                     /locus_tag="IVN13_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(18165..18524)
                     /locus_tag="IVN13_001779"
     CDS             complement(18165..18524)
                     /locus_tag="IVN13_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP7012253.1"
                     /translation="MSCLGNVIWFIFGGLIGGLIWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHIVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18554..18895)
                     /locus_tag="IVN13_001780"
     CDS             complement(18554..18895)
                     /locus_tag="IVN13_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012254.1"
                     /translation="MMEIRWAVKGDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(18892..19566)
                     /locus_tag="IVN13_001781"
     CDS             complement(18892..19566)
                     /locus_tag="IVN13_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP7012255.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
BASE COUNT         5629 a         4158 c         3406 g         6500 t
ORIGIN      
        1 aaagaggggg gaatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tcccttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttag
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttc
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttgt cagctttgat ttgttttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagca aaacagcaga tatcttactt ttctttctga accaaagatc tgtacttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattca atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgggttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgcaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatacaagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacttctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcagccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggtttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagtcacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattac attgaccgtg gattacgact attggtacgc accctttctt
     5761 tctttgatgg cttacttcat gatgcttacc gtttttcgga aaaaagattt actgtacgaa
     5821 atgattgcgg tagtaactgt tttgttcgtg tatctggcag caaggcaatt tttctccatc
     5881 cggttgttgt cgggaaacac tcgattccag tcattgacta cagttctgct tatttgtttc
     5941 ttaactttta gcgctatctt gcctttatat cttccgaaag tacaccaatc tctcgaggaa
     6001 acgagtgaac cgatccgaga atatcttaat gatgaagggc tttatgacac actccatgag
     6061 tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga atgatgaaca gcttggtgga
     6121 cctttatatg ataatggcga agttgttttt acagcgaacc aaaaaggaaa tggctattgg
     6181 aagatcgaaa ataaaaatca ttacaccggt tctggatgga tagaagatca ggacgcaccc
     6241 atccaaccaa tccaagaatt ccctgttgac atcacgagct accaaaccgt tatagaagac
     6301 aaagaaaaca ttacattgac cctaaaaaaa caagctttct taccttatcc ttatggaaat
     6361 actaccttga ctgatttttc attagctagt ggacaaacgc tttactattt gcctgaaagc
     6421 cagcgattca ttcctgtccc gaatgaatcg acacgttctg caactttaca aacagaaagg
     6481 ttagttttta cgagagaaga attaaaaagc agtcagttaa aagctgatga tttagaaaat
     6541 cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac gtattgagga cttggcggtc
     6601 gaattgacca aaaatgaaac aactttaatt cgtaaagtcg aagctgtcga acagtattta
     6661 aaaacaagcg gaaatttccg ctattccaaa acggataccg ggcatactcc gaaagaacac
     6721 gattatgtgg attatttcct ctttgattca aaagtcggct attgtgacaa cttttcaact
     6781 tctatggtcg tccttcttcg aacacttggt atcccagctc gctggacaaa aggttttaca
     6841 aatgggctca gaacaggcac agataatcaa gggaatgagg aatacagcat tctcaatagt
     6901 catgctcatt cttggccaga ggtatacttt cctggatttg gctggcttcc atttgaacca
     6961 accccttcat ttgccaatcc aatgggttca caaccagttg ctgaaacaac cgaaaccgtc
     7021 cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg cagaaactat ttccagtgag
     7081 agatctgagg tcattccatc agaagaaaca acactcacac cagcttataa aatcgtctta
     7141 ttcagccttg gagtgtgttt atccgtcttg ttgttctttc tattgttatt cagaaaatct
     7201 ctgcgctgga caagttatct tatctatctt cattttttta cttctactaa tacgattgca
     7261 tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc gcaaacccgc agaatctttg
     7321 caggaatata gcctgcgaat agaacaagaa ttcaagctag atctgcgttt cattcgatta
     7381 actactcaat atgaagcaga aatatatgga cgacaagcag atccggtaaa attcgataaa
     7441 aaaacctatt tagaaataac gcatataata agaaaaacgg tatacaaaaa aattctgaca
     7501 aaagggagcc gcttcaaaaa ataagataaa atttctggag aatctacttc tggaatgccg
     7561 agaaccagct gattgagtat cgattactcg aaaaaaaggt tgtgacacgt tttttgtcac
     7621 aacctttttt cattattatt tgattttcaa tgtcaaagca tttagagcta caactaccgt
     7681 acttaacgac atcaagacag caccaactgc tggacttaat atgataccaa ttggcgcaag
     7741 aattccggct gctagcggaa ttgcaagaat attgtaacct gccccccacc agaggttctg
     7801 gatcattttc tttcgcgttt gcttggctag atcaagaaaa tgcaaaatat cttttggatc
     7861 gctgtttgtc aatacgacat ccgcagaatc gattgcgata tctgtccctg caccgattgc
     7921 catcccgatt gaagcacggg ctaaacttgg cgcatcattg atcccatcac ccaccatgat
     7981 tacttttttc ccttggtcaa ggtatttctg gacaacagct tctttatcat ctggtaataa
     8041 accgccataa tattcttcga tccctaaata atctgctact gcttgggcag cgtttttatt
     8101 gtcaccagta agcatgacag gagtgatttt tctttctttc aatgttcgga taaattcttt
     8161 tgcttctgtt tttactacgt cacctaaagc aacgattgca gctagatgat tttctaaaat
     8221 caggaagctg atggtatttc cttgttcttg gtaaggtttc aataatgctt gttctacgtt
     8281 taatccgaga cgctcaactt ctttttcatt aactattttc acttgttgtt ttttgactgt
     8341 tgcctctagc ccgactcctg aaagattttt tagattttca gcttgataag gcttgatttc
     8401 atgttctttc aaataattca taatgccaac agccaatgga tgattggcat tcgcttccaa
     8461 ggctcctaga tattgaagga tttcattttt attgaacgca tcatcaagta cttcgacacc
     8521 cgttacagta aattttcctt ctgttagtgt tcctgttttg tccagcatga tcacatctag
     8581 atcattggct tgctccagtg cattgcggtt tttcaaaagt aatccgtttt tagctgctat
     8641 tgatgtggaa cgagcaacaa ctaatgggat agctaaacct agagcatgag gacaggcgat
     8701 gataaagact gttaccatac gttctaatgc attaggaaga tctgtcaaga acaaccaggc
     8761 gatgaatgcc aggataccca caattaatgc gacataaaac agccattttg ccactttgtc
     8821 agataatgac tctagcttgg atttttctcc ttgtgctttt cgcaccattt ccataacctt
     8881 cgctagataa ccattttcac cagttccagt tacttcgatt tcgatcgttc catccccatt
     8941 gatagaaccg ccaatcactt tgtcattggc ttgttttttg attcctcttg attcgccagt
     9001 cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt acaccatctg ttggcatttt
     9061 atcaccagac cggacgatca aacgatcacc ttcgtttact tctttcaatg aaactgtctc
     9121 ttcttttcca tctttcgtca agcgtttcac tgattctggc agtaattctg ctagtttttg
     9181 taaagcattg ctggcattcg acacagcatt catttcaatc cagtgaccta gtagcataat
     9241 aacgatcaaa gtagccaatt cccagaagaa atccatgacg tgtgtatgtg gattgagcag
     9301 atttgcgata aacgaataga cactataaat atatgcaact gtaatcccca tcgcgatcag
     9361 tgtcatcata gcaggacttt tttgtttcaa ttccatctta gctccactta aaaatggctg
     9421 accgccatag ataaatagta tagttgctaa gatcaatacg agccaatcag aaccaggaaa
     9481 cgttacttga aatggaaatt ccattcccat cattggtgaa aaaagaatga ttgggatagc
     9541 taggataagc gatagccaaa atttttgttt gaaattcccc atgtgcatgg aatgatccat
     9601 gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt tgtttgctgt ttgctatatc
     9661 tccatggtta tgtcccatgt gcatggaatg atccatctga ctatggtcat gtttcatctc
     9721 catctgtttt tcatgagatt ctgtactttc tttgtttact acttcttttt tatgctcatg
     9781 ttttttctcc atatcgttcc cattcatttt tactccatcc cttctcttac tttttcatgt
     9841 gtcgatttaa gctcagggag ttcaacaata cactgacgga actaaacgcc atcgctcctc
     9901 ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg aataccaatc gtattataga
     9961 caaaagccca gaaaagattt tgtttgattt ttctcaatgt caacctagac aactcgattg
    10021 tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac atcggctgtt tccatcgcaa
    10081 tgtccgttcc acttcccatt gccataccga catcagctaa tgctaaggcc ggtgcatcat
    10141 tgattccatc gcctgccata ccgacttttt tgccatcttt ctgaagtttt tctacgtagg
    10201 ctgctttatc ctctggaagt acttcagcaa agatatgctt tggatcaata ccaacttgtt
    10261 taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag cataaaaaca tctaatcctt
    10321 tattctggag ctgcttgatt gcttgtttgg cttctagttt gatttgatca gccacggcaa
    10381 tcaacccgat cacttgcttt tcatcagcta aaaacatgac tgtttttccc tgctgttcta
    10441 attctaacgc gtgttcttgg tattcatcaa atgataggtt caattcaatt aatcgtttac
    10501 gtgtacccgc aaaatagcgt actccgataa tcgtaccgct gattccagca cctgggtgag
    10561 ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag ttttccgtat tccacgattg
    10621 ctttacctag tggatgttca gaagcttgtt ctaaagtata aaaaatagat aggacttctt
    10681 tagaaccgac tagatctgta actttgggct ttccttctgt aatcgttcca gttttatcca
    10741 aaacaatgct gtctaagtga gcagctgctt ctaatgcttc tccgccttta attaaaattc
    10801 catttcgtgc accaacaccc gttcctacca taatagctgt tggagtcgct aaacctaatg
    10861 cacatggaca cgcaatgact aatacagaga cactatggat caatgcgagt tgccaatcac
    10921 cagtgatcaa acctgtagct atgagcgtga taaacgcgat gactaatacg atcggaacaa
    10981 aaatccctga gatccgatct gcgatttttt ggattggggc tttgctccct tgagcatctt
    11041 ccaccatttg aatgatttgc gctaatactg tctgcttgcc gacttgtgta acttccatgt
    11101 gaagtagacc attcgtattg attgtcccgc caaagagcgt gtcatccgga ttcttttcta
    11161 caggaagatt ttcaccagta agcatacttt catctatcgc actgcttcct gaaataattc
    11221 gaccatctgc tggaatctgc tcaccaggac ggatgactag ttgatcacca acgactactt
    11281 cttcaatcgc taacgtttct tctttcccgt ttcttatcac ttgggcggtt tttgtttgaa
    11341 gagacatcaa ttgtttgatt gcattacctg ttttcgtttt tgctgtatgt tccaaatatt
    11401 tgccgagcaa aatcagcgtg atgatcatac tgctgctttc aaaataaaga tcttgggggt
    11461 gtccacggaa aaatccgttg taaatgctca atgcaaaggc agccgatgtt ccaattgcta
    11521 ccaatacatc catatttggt gctttcgtct tgattgcatg gtacgcacct ttataaaaac
    11581 gagcaccgac ataaaattgg acaggagcag acagcactaa ctggacgatt ggaaaatgaa
    11641 aaaagtgaac aatcgccgca tggcttccta acatcatagc aatcattgac aacatcaacg
    11701 gcaaagtcaa aaccgtactc aatataagat cgaatagcat ttttttcaaa tacgcttgct
    11761 tttcttctgc aattttttgt ttgtgggctt catcgtacaa aattgcacca tagccgatgt
    11821 tttctacgct ttggattaat ttttcagtgg tcgtcccttc atatttgaca cttgcttttt
    11881 cagtggccaa gttgactgtg gcattgacca caccaggttg tccgttcagc tctttttcga
    11941 tcctagcaga acaatttgca caagtcatac ctgtaatgac gaacgtatcc attttggttt
    12001 ctgctgttgc catcaaatca cctctgcttt ataacccaat ctattgattg cttgacaaat
    12061 cttttcggat tgaacttttg tttcatcaaa cttgaccgta ccgtttgctt ttttcaaatt
    12121 tactttcact ttttgcacgc cgtcaagtgc gctcactgtt tcttctactc ttgctgcaca
    12181 atgattgcag ctcatacctt cgattgaaaa ttgttgtttc attttttctt cctcctattg
    12241 atgttgttta cattcacatt gacctgggat acagttacat tcgatcgtat ctactggttg
    12301 cttttgttcc agttgtttca aaatctcatt gatatcttct tgagtcaaag tggcttcttc
    12361 tactagatcc gcaatcgtct ctccaatcct ttttgcacag atatgggaaa aaagattttc
    12421 cgtcgcactg cgaactgttt ccatctccga gacagctgga tgataaatga atttttttcc
    12481 ttcttgttct gtccagatca cttctttttt taccaaacga cccagcaaag ttttgatcgt
    12541 tgctggtttc caatccatcg aatcagctaa gatctgcgtg atttcttggg ctgtcgtctg
    12601 tccaagtgtc caaataacac gcatgatctc ccactctgaa tcactgatct ttactggcgt
    12661 tttgactttt tccatttatt tcacaccttt cgtttacaaa tgtaatcatt atcttatgaa
    12721 tacagattac acttgtaaac aaaaactgtc aattattttt gctctctatt ttcaatttag
    12781 ttaaaaattc ttgtttctct cgtcttgttt taaggatgat cagtttattt cttgcattag
    12841 ctacttggat cagctgctgt atttttggtt cattgttttc ttcaaaatgc cagataaaag
    12901 ataaaaattc taagtattcc ctgtttagtt tctccgaaaa tgattcaggc atatctggcc
    12961 gagatttttt gtttatcttt ctttttagag aacgccaaac tactcggtag atggcgagat
    13021 aacgcggtac tttaagccaa atgatcttat ctgcttcttt aatcctttcc tctaacgtag
    13081 aagtataatt tccttcaacg atccatgacg gattagatac cataaacgat tgctgttctt
    13141 gcaaaaacca cttttttgcc tgaatactgt agtctgttgt atgccataaa aggtctagcg
    13201 gcagtagcgg aaagccggtg atttctttga tattccttgc gagggtcgat tttcccgacc
    13261 cactttgtcc aatcacgatg atcttcactc ttctccctca cttctctttg gaatttggtt
    13321 cgatcatacc acttataacg ccgcaaaaaa acaagggact gtgacaaaag tcgtgtcaaa
    13381 gtcccttgtt tcgaataaat ggtgttcaat cagctgacct tcagcattag ctcaaaaagc
    13441 ggcaaaatat gaggagctat ttttccgttt gtttctctaa tactcaggtc aaatcgctga
    13501 ttttacaacc tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagcccagc
    13561 caaacgaaaa atatgagggg ctatttttgt ttggctgttc ttattacttg atgcaggaca
    13621 gctttttcac aacctctgat tcacggtgtt ccagaagtag cttcttcgaa aaacaagccg
    13681 aaacttcaca aaaatttgag aagctatttt cgtaaattcc ttcttatttc ttgaagctga
    13741 ccacttctgt cacaacctct tgtaacaagt atttctattt taaggctgaa ccactgtttc
    13801 tggttcagtg atttctttgt ttttgtctcg acgagcaatt gctggcaaga tcatacctaa
    13861 taagatcaag acaattggtg tgatgatatt cgtgaataat tggaaccacc atgctttagg
    13921 atcagcagta taatctactt ttggcaccat tcctaggata catgcaaacg cagtgaaggc
    13981 aaagcaccaa gcaccaaaga taaaaccgat ttttggattt ttaacaaatt tatattcaga
    14041 attgaatttc ttataagctt tattcaacat catatatgcc aagaacaccc ataggtaacg
    14101 cattggcatg acaactgagt tcaagttcgt caaccatttt actaattcat tcatatcttt
    14161 gataccaaag atcggcaaca aaataatgat tcctactagt atacctgtca gaagataacc
    14221 attcaccaat gtacctttac gattacgttt acgtagccag tttggtacaa attctggatc
    14281 cgcatctgct aacaagattt ttagtggtgc atcaattgaa aaggctaatg cggagatttg
    14341 tccaatcatg tttgtcaatg cgtaaatgat catcaatgag ttaccgatac catagtaatt
    14401 acctaaaatt tgaaaagcac tgtatgcccc atttgccatc aagtctttag ggatattatc
    14461 gctagcaaac agcataccca ttgcgactga gcctaataca gcacaaatcc ctaccattgc
    14521 tgctaagaag atcatccctt taggaaattc ctttgcagga tttcttgtag agttgacata
    14581 aggggatatt ttttccgccc ctccaactgc aaatactagc atcgaaacgg ttgtgaaata
    14641 actgaaatca aatgttggaa tatatgtttt cacttgattc atatgtggtg tggcaaattc
    14701 catcgttgga ttgatgaacg gtgccgttac agctaaaaga ataaataaga tcgacatgac
    14761 gaacatcgct gttccggcca gtccgccaat cactttcaaa gtagaaagtc cttttgttga
    14821 taaatataaa aaggcaagaa atataataag tgaaataact gctaccattt gtactgacat
    14881 cgtatttacg atatttccat ttccttggac tgcccaacca ccagcaatca gaattgcttg
    14941 aggcttttgt gccaaataag gaatatggac gacccagtaa gtccaagcgg catagtatgc
    15001 cagtcgttta ttagatgtcg tattttcgac ccatgaactg acgcctcctt tactatcctt
    15061 gaaggctgat cctaattgtc caacgatcaa tgcgtatgga ataaaataaa tggccaaaat
    15121 caatagccaa gaggtgacaa ctgatactcc ttgctgagca tagttattaa ctacattccc
    15181 catgccccaa accatgttaa atgcaattaa cgcaacagta aaccagcgta attgtttgtt
    15241 gttcattcgt tctctccttt atgataaaaa tttgttatcc agatgtaatc atattcaatt
    15301 tttaacaaac ttacaagaca gtgttatttt atgtgtgtat tcttttattt aacacacatc
    15361 gttttttcat attttttcca tcaaaaaaat aagttatttt cttttatttc attgacctta
    15421 ttgaatttca agctattttg aattttctgc aaaattaaaa acattagtta ttttataatc
    15481 ctatttattt tctttacatt tttcattaag aaaaggatat aaaaaaaaga ggacgttcat
    15541 tcgtcctctt cattgatcaa cgtttatttt ttacatgcaa gcttcgcatg gcattcaaaa
    15601 cagctaatac tgtcacccca acatctgcaa atactgcatc tcccattgat gcaaaaccaa
    15661 aagcacctaa ggaaagtacc aaaattttta tcccaatagc aaagacaatg ttctgtttga
    15721 cgattttcaa tgttttcttt gctaaatgga ttgcttctgc aatcttcgat ggttgatcat
    15781 tcatgatgac tacatctgct gcttcaattg cagcatcgct tcccaatcca cccattgcga
    15841 ttcctacatc tgctcttgct agcaccggtg catcgttgat tccatctccg acaaatgcgg
    15901 tctttttatt gttactttgt aagatttcct ctaaccgctg cactttatct tctggaagca
    15961 attcgctata tatcttgtcc acgcctactt gcttcccgat atgatcagca atttttttcg
    16021 agtcgcccgt caacatgact gtgttcttca caccagcctg ttttaattga accaatgcgt
    16081 ctacggcatc ttctttcaag gtatctgcaa taacaagata accactgtaa ctttgatcga
    16141 tcgctagata aagcaatgta ccgatttctt gacttgaagg aaaagagatt tcaaatcttt
    16201 ccatcaattt gtgattacct actaatactt gtttgccatc aacttcagct aagatccctt
    16261 cacctgcaaa ttcttggata ttagctgttg gtaaaagcgg acctgcgtag ccttctaaaa
    16321 cagattgagc aatcggatgt gtagaaaact gttctgcact tgcaacatat tgcataaatt
    16381 tctttggatc aattcctgtt ggatcgatcg tctggatcga aaagtcacct ttcgttaatg
    16441 tgccagtttt atcgaatacc actgtctcag tttgtgcaag caactccaag taatttgaac
    16501 cttttactaa tacaccgatt ttacttgctc ctccgatacc gccaaagaaa ctaagtggca
    16561 cggaaatcac taatgcacat ggacatgaga tcactaaaaa tgtcaatgct cgatagatcc
    16621 attcagaaaa cgccgctcca gaaacaacaa gtggaggtaa aacagctaat aaaacggcca
    16681 atcctacaac gactggtgtg taatagcgcg cgaatttagt aatgaaattt tcagctggtg
    16741 cttttttact gctggcattt tctactaatt ctaataattt gctgatagta gaattttcat
    16801 aagatttttc tacttgaact aaaaatggtt tatttttatt gataaatccg cttaatactg
    16861 tttcaccaac atatacagat ttagggactg attctcctgt aagtgctgac gtatcgacta
    16921 atgattctcc ttcgatgatc gttccatcta acggtacacg ttcaccaggc ttcacaagta
    16981 caaactgacc gatcagcacg tcttcagggt tcacggtcat caatccttcc gccgtctgca
    17041 cgtgagctgt ttctggacgg atcgccatca attcgcgaat cgattttctt gaatgactga
    17101 ctgccaatcc ttggaaatac tcgccgattt gataaaatag catcactgct acggcttctg
    17161 gatattcacc gatcaacata gcgccaatcg tagcaacaga cataagaaaa ttctcatcaa
    17221 agatttttcc tttgacgata ttcatcactg ccttcttcac tacatcatat cctatcagca
    17281 gataaatcaa taaataccct atcaagcgaa ttggttcatt aaaatcaaac aatagcaata
    17341 aagcgatacc tgcaagggaa aataaaatcc ggaacagcgt atttctctct ttttcatttc
    17401 catgttcaga tactttttcc ttatctattt ctgttacttt gacatctggt tctaattcct
    17461 tcactacttt ttttgtttct tgttcaatcg tagccaaatg atcttcctct gcgtcaatcg
    17521 ttagttttcc agaagtgaag tttaccgttg cttccttgac tccattgatt ttctgaactc
    17581 ctttttcgat tttcattgca cagttcgcac aatctaaacc ttctaatctg taacttttca
    17641 ttttaatgac ctcctcgcct caacatatga atatctcttc ataactaatt ataaatgaat
    17701 ggttattcat atgtcaaggt atccgaaaga aaaaatgaaa aacatctgca tcggaaaatt
    17761 tcttccatac agatgttttg gcttattctt tctttttctc ttcgatcatt gccactgctc
    17821 ttatccaacc cgcacttgcc ttcttgatct tgacaggata gcaactaagt ttgaagcctg
    17881 ttgctggcaa tttgtctaaa ttcattaatt tttcgatagg acaatatctt ttttcaatcc
    17941 ctgcaaaatg tccttcccag atatacgaag ctccactacg ctttcaaaaa tatagaaaga
    18001 gttctttaat gaaaattatt tcaactttcc ataaacagta accccatata aataaaagac
    18061 gtaaaagtca catggaaaaa cttatacagg aaaacgcata gagtcaggtg aaatacatgt
    18121 atcccacctg actctatgct ttatctaacc tttattattc tcttttatac aatttctgca
    18181 ccgaatggca tcaacgccaa tcgagcaagt ttgaaagatt gcttggcaaa aggtatccct
    18241 atgatcgtga tacatagaag gatgccgctg acgatatgtg cgagagcaat tggtaggcct
    18301 gaaacgatca gccagatgat attaacgatc aatgacatgc cagactggtg ataaattact
    18361 tctttgccaa aaggcgcaaa agaaagggag gcgatcttaa aacattgcat accgattggg
    18421 atacctatga tcgtgataca ccaaagtatc cccgctgcta accagataag accaccgatt
    18481 agtccgccga atataaacca gatgacattt cctaaacaac tcattgtatt tctccttcta
    18541 ttactgtttt tgattaaaac tgggcaaaaa ttgattttat tcttcttttt ttagcttcca
    18601 gcatcaagta ttctgcaaat tttttttgga tttcttccgt attttctttt gttactaatg
    18661 tgtgttggat tagttttcct gatggtcgct gatccaatcg ttcttctgac tgttgcacgt
    18721 ccgcttgcgc aaatccgata atccgttttt cttttgttac agcaacgagc aatagtttat
    18781 tcttatcatt taggtcagct gccgtttttg cttttacgaa tgattctgga tgatcagtaa
    18841 tcagctgatt gatttgaggt aaatctcctt ttactgccca ccttatttcc atcatttttc
    18901 acttttcttt cgctcatttt tatctccact aaaaaaatcg actgcggcaa ttgcatctat
    18961 ccagctttgt gcaatgatct catgtccgcc aatcgttggg tggaccccat cttctcttgt
    19021 atatttcgaa aaaccataag caatccctaa agcattgaat ataccatcta aaggaaccag
    19081 caatgcgcca tattccttag caagtttacg aacaatttgg attttagggt ctaagtcttt
    19141 acgccagcca tgacggtcgt cggttatagg aagtacaaac ggctccatca agatgatgtt
    19201 gcagatttcc gctgtttgaa gttcatttag taattttcga taaacggatt caaattgctg
    19261 agcagatttc tcagttccga atatagcctg atcgctagta ttatgccagg tatcattgat
    19321 tccgatcaaa atcgatacat agtcaggttg taaagctaaa caatcttctt gccagcgtac
    19381 ttgcagatct gcaattttgt ctcctcctat cccacggttc atgcattcaa attccatcgt
    19441 tggatattgg gagattagtt ttccggcaat caaaagtgga taacctttac ctagatcagt
    19501 tgcttctgct cggtctcttc ctgcgtctgt aatgctatca ccgataaata atatacgttt
    19561 tttcatttgt caaaaactcc tttttgaata aaacgatgat tttcttcctt atattatccc
    19621 ataagcccta tcaagacgaa agagaaaata tatgtataaa aaatgacacc agcctagtcc
    19681 cttcaaacaa ttg
//
LOCUS       DACCKU010000053        19543 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.056, whole genome shotgun sequence.
ACCESSION   DACCKU010000053 DACCKU010000000
VERSION     DACCKU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.056"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(443..1396)
                     /locus_tag="IVN13_001782"
     CDS             complement(443..1396)
                     /locus_tag="IVN13_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7012256.1"
                     /translation="MKKLVVGILILASFGLAACGNSDNTSQADTKKSSTQAETALTIK
                     DSNGDQIEVPKNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(1419..2174)
                     /locus_tag="IVN13_001783"
     CDS             complement(1419..2174)
                     /locus_tag="IVN13_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7012257.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKKLVQEIGKTIVIVLHDINFAASYADEIIAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(2171..3130)
                     /locus_tag="IVN13_001784"
     CDS             complement(2171..3130)
                     /locus_tag="IVN13_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7012258.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFAWALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTFLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(3123..4073)
                     /locus_tag="IVN13_001785"
     CDS             complement(3123..4073)
                     /locus_tag="IVN13_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP7012259.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFPQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            4223..4675
                     /locus_tag="IVN13_001786"
     CDS             4223..4675
                     /locus_tag="IVN13_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP7012260.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPVEKEKPKKKKSFWDRLF"
     gene            complement(4763..7030)
                     /gene="gshAB"
                     /locus_tag="IVN13_001787"
     CDS             complement(4763..7030)
                     /gene="gshAB"
                     /locus_tag="IVN13_001787"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP7012261.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEAIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDGIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(7217..8215)
                     /gene="ftsY"
                     /locus_tag="IVN13_001788"
     CDS             complement(7217..8215)
                     /gene="ftsY"
                     /locus_tag="IVN13_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP7012262.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKIEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(8227..9036)
                     /locus_tag="IVN13_001789"
     CDS             complement(8227..9036)
                     /locus_tag="IVN13_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7012263.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(9045..12626)
                     /gene="smc"
                     /locus_tag="IVN13_001790"
     CDS             complement(9045..12626)
                     /gene="smc"
                     /locus_tag="IVN13_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP7012264.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLLKEVAEKETEIQKAEASLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYVQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPNQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(12646..13332)
                     /locus_tag="IVN13_001791"
     CDS             complement(12646..13332)
                     /locus_tag="IVN13_001791"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7012265.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(13680..15461)
                     /locus_tag="IVN13_001792"
     CDS             complement(13680..15461)
                     /locus_tag="IVN13_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7012266.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(15487..16401)
                     /locus_tag="IVN13_001793"
     CDS             complement(15487..16401)
                     /locus_tag="IVN13_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012267.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(16428..17390)
                     /locus_tag="IVN13_001794"
     CDS             complement(16428..17390)
                     /locus_tag="IVN13_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012268.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(17390..18334)
                     /locus_tag="IVN13_001795"
     CDS             complement(17390..18334)
                     /locus_tag="IVN13_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012269.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(18335..19339)
                     /locus_tag="IVN13_001796"
     CDS             complement(18335..19339)
                     /locus_tag="IVN13_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012270.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         5390 a         4233 c         3465 g         6455 t
ORIGIN      
        1 tgcgatttgc tttgagtaat aagaacgctg gacagaaaat agcttctcat attttctagt
       61 ctagtggact tattgccgag aagcagcaga tggaacatcg tttaaagaag agatcggtac
      121 aaaataaaaa tatctaaatt taagttttat ctaagtttcg ctttgtacct ttgattcaaa
      181 tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac tattttttgc cactccccat
      241 tctagaatca acccgtatga tgtagagaat gaattcacgt tgaaaaaaga tttcccaaat
      301 aaataaaaac catccgtaaa atcatcaata cgattttacg gatggttttt gataacttct
      361 ttgaggatag gctcttgcta tttaagaatg taagaaacat tattgttaca tagtatgaga
      421 gtttaatcct ctgttattta tgctatttaa atgcttgatt cacatcttcg atcatcagtt
      481 tcattgattc taaaccgccc ccgcttaaat accagacatc tggttcaagg gagatgatct
      541 gctgattttt accagcattc gtttgtgcta ccagttcatt ggcagaaata tcatctttgc
      601 tgtcgtctcc accaatagct tttgttctgt ctactacaaa taagatatcc ggattttttt
      661 ccagcacata ttcataagaa acactttgac cgtgagtgga tgcttcgatt tgatcatctg
      721 cttgttcaaa gccaaataaa tcatggatga aaccgaatct agaaccagat ccgtaggctg
      781 ataattgccc ttcattcaca agtgtgacta gtgctttttt gtcagtagct tctgctttct
      841 ttttcgtctg atcgattgca ctagataatt cctctaattg agttttagct tcttcttgct
      901 taccaaagat ctcccctaaa gcagtgacat tttgctggaa agattcccat ggatttttcg
      961 catctaaaga aaggaaaatg gtcggagcaa tttgacttag atctttttga aaatctcttt
     1021 gccggccaga aatgatgatc aggtctggct gaagctgatt gattttttct aagtctggtt
     1081 ctttgatccc acctgctgat tccacttttt tataggatga aagatactca ggaaggctgc
     1141 ttgtagctgc tccaactacc ttatctccaa caccaagagc atccatcgta tctaaagaac
     1201 cattgtcgaa tacgacgact ttttcaggat tctttggcac ctcgatttga tcgccattac
     1261 tgtcttttat cgttaatgcc gtttcagctt gcgtactgct tttctttgta tctgcctgac
     1321 tggtgttgtc actatttcca catgctgcta aaccaaatga tgctagaatc aagataccta
     1381 ctactaattt tttcatctct tctctcctaa tgttagtttt agaaataaag acagaatctg
     1441 cgaccttcaa tctcacaaat tcgtatgtcc atttcgtaca attcgtccag tacatctttt
     1501 tttatcattt cttgggtcgt tccttgcgtg aataactgcc catctttcat tgcaataatt
     1561 tcgtctgcat aacttgcagc aaagttgata tcatgaagaa caataacaat tgttttcccg
     1621 atttcttgta ctaatttttt taagatctgc atgatttgaa ccgcaaaatt catatctaga
     1681 ttgttcaaag gttcatccag taaaatataa tccgtgtctt gcgctagcac cattgcaata
     1741 tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg attgatccgc tatttcttct
     1801 aacccaaggt gttccagtga ttcctgaacg atttcctcgt cttttgtcgt caattttcct
     1861 ttcgtataag gaaatcgtcc aaattctact aattctttca cggtgatttg gacatttatt
     1921 tcattcgatt gtcgtaggat cgataatttt ttggctaatt cattggattt ccaagccttt
     1981 acttcgttat ggtctaaata gatttcacct gtatcttttg gtatcaaacg actgatcatc
     2041 gacaacaccg ttgactttcc agcaccattt ggaccgataa aagcagtcaa cttcttttca
     2101 ggaatcgtta actgtacatc tttaaccact gattttccat ggtacagttt attgaccttt
     2161 tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa ttttgtgaca aaataaattc
     2221 ctccgacaaa ttcaacgacg acactcactg ttgtattcca gccgaaaatc tgctctactg
     2281 cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa gcttccgata aatagctgac
     2341 catgacgata agtagaaaag acacgataac tgatatttgc gacaataaac cctaaaaaag
     2401 taactggacc cacaagagcc gttgatgtcc ctgtcaaaag actgacacaa cataaaatca
     2461 aaaattcaaa acgatctgtg tctatcccga ggtttactgc ttgttcattt ccgagatgga
     2521 aaacgtctag cacatgtcga tatcgaaata acaggtacca gcagccgata atgatcaaag
     2581 aagcaggcaa aagatactga ctctggatat tgccaaagct tgcaaataat ctgccttgaa
     2641 gcaaatcata ttcattcgga tccatgatga cttgtaaaaa ggtactgaca ctaccaaaaa
     2701 gcgtacctaa aatcatccct gacatcagta ataaaaaaag attcgctctc atttttttca
     2761 aaagaaagaa aaataacaat agactcagcc cgaccatcag cccaacgttc aataagaaaa
     2821 aaactgtacc tgtctgctga agcatcgtca ctcctccgat aaagaagaaa aggaacgttt
     2881 ggacaaggac atataacgac tcaagtccca atactgaagg agtcaaaaac tggttatgcg
     2941 taattgtctg aaagctgatt gtggcactgc tgacagctag cgagacaagg ataaatgcaa
     3001 aaattttctt actcctcaat gcccatgcaa aatgccagtt tccatatgta tggatcgtca
     3061 agtacattcc tgtaacaaat aaaacaagaa caagcatacc aataaaaata gatggctttt
     3121 ttttaagcat atcgtcgccc ccttatcaga agataaagga agatagcccc accgatgatt
     3181 ccaacgatca cactgactgg tatttcataa ggaaaaatca acacgcgagc gacaagatcg
     3241 caaaatacta agaacaaact acctgctaaa gcagttacgc caattgtttt gtgcacttga
     3301 tctccatata ttctagagac aagattcgga atgatcactc cgataaaagg aatgcttcct
     3361 accatgatca acatgactgc actagccaat gatactaagc caatgcctaa aaactgcatc
     3421 ttctggtaat ctattcccaa atttactgca aacgattcgc ccagaccaat gatcgtaaat
     3481 cggtaagcaa atagccaaac caagagcaac aatggaacaa cagcatacaa taactcataa
     3541 tttcctcgta tcacagtcgc aaaatttcct tgaagccatg aagtcatatt ttgtaccaac
     3601 tggaattgat aagcaaaaaa agtagcaact gaaccaatga tattccctaa catgactcca
     3661 atcaacggaa gcatcaattg atttttctgg gggaacaatc gcgtaaggga taaaaaaagc
     3721 aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc ggacaatcaa ggatgcacca
     3781 ggtaggaaaa tcatagccac taaaatacct aaacgcgcac tatccatcgt gcccgcagtc
     3841 gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga tcaacccaca aatactactg
     3901 gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac gagtcactag caagatttgt
     3961 aagctttgcc catctaactg gagcagttct gacaattgta cattcgatac tccgatgaac
     4021 aatgaaatac tacttaaaat aactaataat gctactagtc gtccgtagcg catccaatcc
     4081 acctttctaa ttgagaatga ttttcattct tattttaagt gaatcgttga acaaaagcaa
     4141 ccgctttttc atatgtttta ggtattattg ggaatatgct ataatcgtaa gaaagacaaa
     4201 gagaagaagg agggtatttt ttatggcaca agatgatgta aaaagccatt tggataaggg
     4261 actttatggt acacctttag tgaaccctga agaacagcat aaatatttag ggacattcag
     4321 ggaacgctgc tatcttagta tgactgttgc tgaaatgatc caaccaaaaa acaaagaaaa
     4381 ctttttaaaa gaactcaaaa aacatccaga tgcttccata ctcttgaatg gggctctacc
     4441 cctttctgtc caaaaccaat atatccagct agcaacgaaa aacaatagcc gatttactgt
     4501 agtcaacgat tacgtcactg caaatcctga ggaattcgga ttgctcttaa cagcaaaaga
     4561 agcagtaaat gaacctgtga ttgatatcga aaaaaaatac ccagaagaaa actcttcgcc
     4621 agttgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg gatcgtttat tttaaaacga
     4681 aaaaacacat aaatttttac atgcaaaaaa gcttttgatc agattgacat caaataggac
     4741 aatctgtcaa aagctttttc ttttaaaata cttcaggata taacaatttc aatacattca
     4801 tcgtcaatct tctgcctttt cctgcaaagg gataaacatg catatgcatg gctggattga
     4861 aatttgcttc gatgatccca tatgcttttt tatcagttgt aggatcgatt tctttatctg
     4921 gtataatcaa atcgatacca ctaatcttag ctcctagtgc ttctaccgca gaaacggcga
     4981 tttttttgta agcttcagaa aattcatcag tcatatcgat cgaatcacca cctgtactga
     5041 tatttgagtt ctcccttaga taaacaattt gattggcttg gggaactgat tcgatcgtca
     5101 acccttgttc tttcaacatc aattgttcta attcacctaa ttggatcaat tctaaaggtg
     5161 cacgatgatt cgttccacgc aaagggtcac tatttttttc ttcaacaagt tctttcactg
     5221 ttcggatacc gtcaccaata acattagcag ggactcgcaa catgatggct tggacttctc
     5281 cgtcgatcac aaagaaacga tattccgttc ccggcataaa ttcttctact aaaaccgaac
     5341 tgtcttcacg aaaagcaatg gctaaacctg cttgataatc ttccagacta gctccttcct
     5401 tgaaaatcgt gatacctaac ccgtaattgg ttgatttagg tttgatcaca aatgcctgct
     5461 cggcaaatct cggataagct tttaccgctt cttccatgga tgaaaattct gcgcctcctg
     5521 gtacccggaa cccagcttct tttaagactt ttttcgttac tgttttgttt tccatgataa
     5581 gtgggacgat atagctgtcc ttacttgtca tgtttgcatt tttcacatat tctacatggt
     5641 cttggaattg cagacgcaaa aattgatccg actcatctaa tactttgact tggacacctt
     5701 tttggatggc atcgaacata aatatctgag tagaaagttc catttcgcga aaaccggcca
     5761 actgataggg acgttcatgg ctttctttat aatattgatg tcccaatgcc acagcaactt
     5821 cttgctgact cgatagctgt gcttcttgat agagtttcgc tgctaacgtt tgttcaggat
     5881 gatccatttg agtcaatgct tcctctacta aataaagaga atccaaccaa tccaattcaa
     5941 ccagcatttg cttcatccct tcaagaattt ctctaccttc ttgatgaaat tctgtttgat
     6001 ctgatggttg ttctagtgct actttgttat ttcgagtcgt accttctgct acccattggt
     6061 cacagtcttc tttttcttct gtccataaca tgtacatcag gaataattgt aagaaccgaa
     6121 cttgctctgg attgataccc aatcttgcat aaggattcaa atcgatattc cgcaattcaa
     6181 tataacgaac acctgttgtt gctaaatctg cgactttctt tccgcctcgg aaacgaagcg
     6241 gtgtataaaa ttctttctcc tccgaaagtt tcccttcttc gatcattcgc tcgatatctg
     6301 ctaaatactg tttcatagaa gcataggaaa ctttcacatt tggatgattc gtatagccca
     6361 gcgcactgtt acgaatactg cgaacaggtt cttgcggatg tgattcatct aaaaagtagt
     6421 tcttctcact cattggtgaa gcaccgaata aataagtgat catccagcga tagcgcataa
     6481 agtttctcgc cgttttcaaa taaagttctg ttttgaattc ggaaaaatct tgaaattctt
     6541 cctgatggct gaacaatgtt cttagcaact catcgccaaa ttcaaaattg aaatggatac
     6601 cgcttaccat ctgttttcgc ttcccgtatt cttttgctaa ataacgtcga tacaagacat
     6661 cttcgaaatt ttttaatttt gcaataatga tcgcttcgtc tttttccggt aaggcaggtg
     6721 gcatacttaa tggccagatc atctcttctt tcggtataga acgagcagtc acatcataaa
     6781 cagcagcgag atactgaaat aattcgggaa tagaatcggt aacaggagtg atcatctctg
     6841 tttgtgtttc gctaaaatct gtttggatat aggggtgata ggatcgatcg ccaaagattg
     6901 ctggatgatc ggtttttgct agatttcctg caagatcaac ccgctgtcct tcccgttcta
     6961 taccaaatcg agcttggtcg ataaaagggc gcgcattgac atgcaataat aattgcttaa
     7021 aattcatcat taagtgacct ccaattcata tgaatctaat tataatcaaa aagtctaaga
     7081 aaaagaacga aattgtttga gaaccgctta caaataagta taaaaatgaa ggcatcaaaa
     7141 acacagctat gttaaaaaca atcattcaaa caaggagctg caacttttgt cacagctcct
     7201 gctttttaga catgggttat tcttctttta gtaatccttt gaacaatcca acgacaaaat
     7261 catttggatc aaacggctcc aagtcatcga ttccttcacc aagaccgacc aatttcactg
     7321 gtaaatgcaa ttcattacgg atagcaagta cgataccgcc ctttgctgta ccatctaatt
     7381 ttgtcaaaac aagaccggtc acatctgttg tttctttgaa ttgttttgct tgaaccatcg
     7441 cgttttgacc agttgttgca tctagtacaa gaagtgtttc ttggggtgct tctggtaatt
     7501 cccgctggat tactcgtttt attttttcta attcgttcat caagttcact ttattttgca
     7561 gtcggcctgc agtatcaact agtaaaacat ctgcctgctc tatctttgct ctttccaaag
     7621 catcgaatac gacagctgct ggatcgccgc ctgcattgcc acgaacaact tcaacaccag
     7681 cgcgttcgcc ccagacaact aactggtcga ttgctccagc gcggaaggta tctgctgctg
     7741 ccatcaatac ttttttacct tcgtttttga actgatgggc aagtttacca atacttgttg
     7801 ttttccctac accattaaca ccgacaaaca aaataactgt tagtccgtct tcttggatat
     7861 ttagttcatt tacttcattg attccttccg cttcatagag atccacaagt ttctcgatga
     7921 tcgcattttg gacttctgcc gattttttcg catttctgag tttcacttct tgacgtaatg
     7981 cttcggtgat tcgtatcgct gtttcaaaac ctacatctgc accgataagt gtttcttcta
     8041 attcttcaaa aaagtcttca tcgactgttc gaaaattggc aaacaactca ttcatccgtt
     8101 caccaaatgt tttacgcgtc ttttctaaac ccttgtcgta cttctcctgt acttcaactt
     8161 cttctgtttt ctcttcgcca gtaaaagcac gcttgatttt atcaaaaaat cccatttgac
     8221 tcccccctac tctaatacaa atttttggat cactttggct acgccatttt cttcattgct
     8281 ggctgtcacg aaatctgctg cttctttgac gaaaggcaca gcattctcca tcgctacgcc
     8341 tagtccagcg tattggatca tcggtaaatc attttcttca tcaccgattg ccatgatttc
     8401 ttcttgtttc aaaccaagat cttttgccaa taaagaaata ccataagctt ttgtaacacc
     8461 ttttggcata aattccaaaa gcatacttct cgttttgatg atctcataca tttcatggaa
     8521 ttctgctggt atttctttga ttttaggatc taattcttct tggtcataag cgattactgc
     8581 cttattaagc acaaattcat ctgtaatatc gcttatatcc gctggttgga attgcagcag
     8641 cttattcaac acattgtaca aggattgctt ccttggggca ctaggcagct gtaaaaccac
     8701 attgtcagac aaaacatcta gtggcagatc caattgctga gccagtttgt ataagcgttg
     8761 gatatctgat acttccatca ctttcttttc catgatttca ccagtatcat ttttttgtac
     8821 caaaccgccg ttgaaagtga tgctgtagtc accttcatct accagtccta attcttgcag
     8881 ataatgcgcc atcgcagcta aggggcgtcc tgtacataga acgatcttga ccccttgctg
     8941 ttttgcttgt gctaatgctt gtttattctc atctgaaatc agcttttgtt catttaataa
     9001 tgtaccgtcc aaatcgattg ctactaattt gatcattttc gttcctacac tttcgtttcg
     9061 ttgattttcg cctcttcatt gatatcttcc atccgtacag aaacgatttt tgatacaccg
     9121 gattcctgca tcgtcactcc gtacagtaca tctgctgcga ccatcgttcc tttgcggtgt
     9181 gtgactacga taaattgtgt gtcatcttgg aaatcgctca agtaacgacc aaaacgtttc
     9241 acgttggctt catctaacgc tgcttctacc tcatctaaga tacaaaacgg aacaggacag
     9301 acacggataa tagaaaagag caacgcgatg gctgtaagtg ctcgttctcc tccagaaagc
     9361 aaacttaaac tttgcagttt tttgccagga ggttgtactt caatctcaat acctgttttc
     9421 agcaaatcag ttggttctgt cagtactaat tcagctttac cgccgccaaa catatttgga
     9481 aagacaattt tgaattcttg acggatagct tcaaatactt ctttgaatct tgtccgcact
     9541 tcatcatcca tctcatccat cgtttcaaac aattggtttt tcgctgctaa cagatcatct
     9601 cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt cgtattgttc gattgaattt
     9661 aagtttactg gtcccagttt ttcaatctgt tcttttaagg atgaaacctt tgttctactg
     9721 tcttcgatat cggttgtttc ttgatagtct tgggaagctt tctcaaatgt cagctgatat
     9781 tctgactgca gataggtcaa atgattatct aaaatcattt cagctcggtc tttttggact
     9841 tctaatttgg attgttccga cagtcgtgct ttttgctcac gattacgctc tgccaagact
     9901 gcttcaagct tatcgatctc cttttgcctt ctatctcgct gctctttggc ttttgccaac
     9961 tcggctttca atgtttctcg ctgagcagat aattcgttaa tttgtttttc caaactttct
    10021 tctgtgactt catgatcaga aaagtctgct gtcaatgtcg ccaactgctt ttctatcgct
    10081 tcttgacgtt cggttgcctc tgctttttgg actcgggcac cgcggagctg gatctgcaga
    10141 tgattaaact gctctttcaa gacagcaaga tctgcttgtt cttgtgcttt ttgactttgt
    10201 acttgtgctc ttctggcttc catctggtcg ctttcttgac ttaacgattt gatttcttcg
    10261 tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt ctttcaattc gttcgtttgc
    10321 ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct caaaagttga gatttgcttt
    10381 tctttttcaa aacgttcaag ttcatttatg atgttttgca gctgatttgt cgcttcttgt
    10441 tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt cttgttcttc ggctaaacga
    10501 gctgtttctt gttgcagctc ctgaactttt ttttcttgtg cttggagttg tttatctgct
    10561 tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt gtacaaacaa actgccttgg
    10621 tttcctcgtt tattcgctcc acctgtcatg gatcctccgg cattcatcac atcaccttct
    10681 aaggaaacca ctcgatattg atagcggatc gtttgcgcga ttgcattggc actagttaag
    10741 tctttggcta aaaggatcgt tcctagcagg ttttgaacca cagtttggat ttgattggga
    10801 taactgactt gttcactggc aatccctata aacccttcta cagctgctgc ttgtgtcaat
    10861 atatgagccg gtaattgtct tggtttgatt gtcgtcagtg gaaggaatgt tgctcttcct
    10921 cctctttgct gtttcaaata cgtgatggct tgccgagcat ctttttcatt ttcaacgatg
    10981 acatgctggg ctgccccacc taaagctgtt tcgatagcta acgtgaaatc tgcaggaaca
    11041 tcgatcagtt cagccactgc tccgacaata cccgaaagct gctgtttatg ctgcaagacc
    11101 aatcgaaccc cttggtaaaa accaaaatag ttttcttgga tttcttgaag gcttttctta
    11161 cgagcacgta gctgctggac ttgattcatt aattgataca ttttaggctg ttcttttgct
    11221 aatgcttctt ggaacttttt cccgttttgt tggatcattt ctaacttctc ttttgtttca
    11281 gttaaagatg attttagctg cgcctgcttt tgtgtcaatt cttctttttg taagatcaat
    11341 cggtctactg atgcctctac ttccgattgc ttggctaacg tctgcttact ttttgctgtt
    11401 tcttgtacat attgacgttc tagatacttc agttcgtttc ctacagctgc ttgctcttgc
    11461 atcaaatcaa catattgatc tcttagttct gccagtaatt ctttcgttga tttttggtat
    11521 ttttctaatt cttgctgtgt ttttatgaga cttgcttcag ctttttggat ttctgtttct
    11581 ttttctgcta cttctttcaa caagctttct tgaagctttt caaaatgttt aacctttttt
    11641 tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt tttgtgtatg ctttgtccgc
    11701 tcctgcaaaa catctttttg tccttctgtc tgcttcagtt tttccgaaag atctaataat
    11761 acttgctgat ttttttcgat cagacgatcg gcttgcgcat tttctttacg ctgcttcgcc
    11821 aaaatggatt cttgttcttg gatcgactca gatagtttgc ctaattctag attgaatttc
    11881 gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg tcttgatttc ggcaaccatc
    11941 aaagagacat cggtttgagt cagtgtttct tttagccgta aaaactcctt tgctgcctca
    12001 ctctgggcgg ccaaaggagt taactgttct tccaattcgt ggatgatgtc ttggacgcgg
    12061 ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt tctttttccg ctgcttgtat
    12121 ttcaagacac cagctgcttc ttcaaagatc ccacgacggt cttccggttt actactgaaa
    12181 atcgcttcta cttttccttg agaaatgatt gagaacgatt cttttcctaa tccagagtct
    12241 aagaacaatt cttggatatc ttttaatctg cagctttgtt tattgatgaa aaactcgctt
    12301 tccccagtcc gtcgataacg gcgggttaca ctgatctcat tgtactccaa tggcagataa
    12361 tgatcactat tgtctaaaat cactgtgact tctgcaatat tcagctgctt acgggtatcg
    12421 gaaccggcaa agatgatatc cggcattttt ccgcctcgca gacttttagc agattgttct
    12481 cctaataccc agcggatagc ttctgtaata ttgctttttc cactgccatt tggtccaacc
    12541 actgctgtga cgctattttc aaagtcaatg actgtcttgt ctgcaaatga tttaaaaccg
    12601 gctatttcaa ttcgtttcaa atacaaagct ttccctcact tacttttaca gtgctgccaa
    12661 tgcattttcc gccgcagctt gttctgctaa ttttttcgat tttccttgtc cagtgccaat
    12721 caattgatca tctacataaa cttcgatcca aaatactcgt tcatgagctg gaccttcttc
    12781 attgatcaat cggtattcga ttgatacatc acctgatttt tgcaagactt cttgtagttt
    12841 tgttttatga tccatctcat gtgaaaaagc acctgctttg actttaggaa aaatcacttt
    12901 ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga tataacgcgc ctaaaaaagc
    12961 ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct cctgaattct cttctccctt
    13021 gcccaacaaa acatattgat caaagtgaca ttctttggca aatttcgaca agctgtcttc
    13081 gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct ggcaaatgag gatataaacg
    13141 gaataaatac tgagaaacca tcagttctaa aacggcatct cctaaaaatt caagtcgctc
    13201 attatccgat aattgcagat tgcgatgctc attcacatag gatgaatggg taaaagcttg
    13261 ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt tcttttagtt cttttgtcag
    13321 ctggttgtcc attttccaca ttccttttat ggatattttt tcgatacttc cttattatac
    13381 tggttttttg tcaaatttga aatggttcgg tcaaaaggta agtaaagctt taataatcct
    13441 agtgattgac gagaagttgt gaaaaattcg tttagtttcg agtatgaaga aaaaattttg
    13501 tgaaaatggc tttccatatt tttgcataaa tttgacttaa taccgagaaa ctggactttg
    13561 aacaccgttt attggagaag tcatgaaatc agcgccttga cctgaatatt agagaaataa
    13621 acagaaaaat atcaaaaaac cgaaaaagga catcgccctt tttcggtcat ttttagtgat
    13681 tatgaacgtg aatctgctgt aacagaaaca tttgcccatg ggtttggtac atcataagac
    13741 caatcccagt ctttgacacg gtcgttgact ggcaatactt cattacggaa caacgttgga
    13801 atcgcaaatg cttcttcaaa tgcatattgt tgccattttt gatatgcttc tttttggtac
    13861 tcttcatcaa atgctttgct tgaactgata tcgttgatca attttgtatt ttcttctgat
    13921 tcataacgag tgtagttgaa cgcagcattt ggtccccata gtccacttgg atttggatca
    13981 gttcctgtat tccaagcagc ttggtaaaca tcaatttcag gatcatcgtt ttctaattta
    14041 tcatagaaag aattgaattc gatcagacga cctgttgtgt attggacatc caaaccgatt
    14101 tctttccact gttggatata gtaatctgcc aatggttgcg ctgtttcccc accggacatc
    14161 gaagcaaagt tgattgttag tttatttcca tctttgtctt ctcttaaacc atcgtcatcc
    14221 acatctttgt agccagcatc atctaatagt tttttcgctt tgtctagatc ttgtgtgaat
    14281 cctttcatct catcatcatg gtatgactgg aatactggag gaatcaatga agtcgcattg
    14341 cttctcaaac ctgcatagaa acgctcacct actgcattat tatctaatgc atagcccatt
    14401 gcttgacgta atgatttatc agccattttt gaatctggat tgtattccac actctttgta
    14461 tctgcattcc atttacctaa cttgaatcct agataagtat aagaagtttg ttcacggcct
    14521 aagtttgtat agccagcaat ttctttatag gtatcgtaag tatctgttgg catttggaaa
    14581 atcatatcat ataatttaga gttcaatgct tcagttgcac ttgccatcgg tacagatttc
    14641 atcgtgatct ttttcaattt aggcgtacca ttccagtagt gctcgtttgg caagtattct
    14701 actgattctc ctgcaacgat attactcatg taataagggc caaaagtcac tggattttta
    14761 cgtacttgat cgcttgattc catatctttg attgctacac cttctaacgt atgttttggt
    14821 gctgcatatg accaaatacc accacccgat tgaagcatgc ttgggtttac ttctttatat
    14881 tcgatttcaa ctgttttgtc gtctattttt ttgattccgg agattgtgtc ggcacttcca
    14941 tcatgatatt cttccatacc tacgatgtta cggaaagtgt cgtcataacg gataccagtg
    15001 taatctttat tcccaatgat ttcgtaaggg aaaatcacat cgtccgccgt tacatcttca
    15061 ccatctgacc atttgacgtt gtcttttact ttgattgtcg cttttttcgt atcttcatct
    15121 agatcaagcg ttgcagctcc gtcgtctgtg attctgaaat cgtcatcgtt tgcaaatagc
    15181 ggttcatgag aaggagccat aaaagctgca tcatatgaat cttggtaaaa ctcccactgg
    15241 aacaatcctt taaactgact gtccgttgcg accgcagctt ctaattcgcc gtcttcgatt
    15301 gcatcatcat tattttttgt tgcaattgga aaagttaatt cctctttgtt attgccgttg
    15361 cctccagagt tgtttccaga ggtatctgta cctccgcccc cgcatgctgc aagaacgaca
    15421 gcagacatca gcgctgcaaa tccaaaaatc gttttcttca ttcctatttc ccccttcgct
    15481 tctatactag cctaaccgtt ggcgcgcatc tgcagaacgc ttgaatgctt gtccaacata
    15541 attgatactc aacatcaaaa ctaaaataag gattgatgcg ggtaaccaga tccattgccg
    15601 attcactaag acttctccgt tacttgcata accgatcaat gtacctaaac taggtacatt
    15661 tgccggcaag ccgaaaccta agaaagtcaa agttgtctcg ataccgatgt ttgccgcaaa
    15721 gttcatcgtc aaattagtaa tgattaatga acttagattc ggcatgattt cgcggaacat
    15781 gattttaaaa tcattggttc ccattgtttt tgaggcactg acataatctc ttcttgcttc
    15841 agataatgtc ttgcttcgga ataatcgggc tttagctacc caataaaatg cactcatgat
    15901 gaatataaag gaccagacat tgtatctgga aataatcgat acgaagacga taatgatcag
    15961 catgatcggt agaatcatga taaaatcaac gatccgcatc aagatgttgt caatgatccc
    16021 tccgtagtag ccagagatga tccctaatcc aaccccgatg atcgaagtaa ggatcgtgat
    16081 cgcaaatccg atcgtaacag agtttctagc acctatgatc aattgtccta atacatcacg
    16141 tccgccttca tctgctccca gaatgaactt ttgtccttgt gtcgaaactg ttcctggtgc
    16201 agcgtatttg tcgaagatac taacatacat cactttgtct tgatcggtca gcatcgcacc
    16261 gataaagatc actaataata aaaccactag caaacctaat gaaaaaatag caattttatc
    16321 ttttttaaat tctcgagcaa tcattcgtat ccccatagga ggaatacttt cttgaactgc
    16381 tgtattgttt ttttcactca tcgatccatt ctcctttctg attggtttta ttgaatccga
    16441 atacgcgggt caacgatact catgatgata tctgaaagta atgtccccac tagcgtcgcg
    16501 attcctaaaa tcaatactaa ggatgtgatc acggtatagt cccgttggcc gatggaatca
    16561 ataaatagtt ttccgactcc cggataacca aaaatctttt cgataactac agaaccagca
    16621 atcaacgaag tgatttcata ccccatttga gaagccatcg gcaacgctgc atttcggaaa
    16681 atatgtcgac tgaatacttt attcgtcggt acccctttgg aacgggctgt tctgacaaaa
    16741 tcgagcgatt tcgaatcgat tacttcactt cgcagatatt ggattgtgac ggcagtcgcc
    16801 aaaaatgctt gggtaattga tggtaacaga atatatttca aacggtctat ccattgagtg
    16861 ataaatccac cttgcaaacc tagtgtttgt gatccacttg ttgggaacca gcctaaacga
    16921 taaccaaaaa caaatagcat gatcaagccg aagataaata aaggaacagc aaaactaaag
    16981 aagttgtaga ttacgacaat cttatccaag attgaatttt gataccggcc agcaagcatc
    17041 cccagaggca cagcaatcaa ataagtaagg acaactgtca agatcgatag atataaagtg
    17101 ttgatgatcc gttcgccgat caagtcagca acagagcgtt tgtataagaa actttgacca
    17161 aaatcgcctt gcaaagcatt tccgatccaa cggaagtact ggacatacca tgggtcattc
    17221 aagccagctg cttgtcgcat ctgttcgatc actgccggat cctgatttgg attgatcaaa
    17281 ccggtaaatg gatcccccgg catcgcttgg gctaataaga agatcagaac acttaaaata
    17341 ataacttgcg ggatcatcaa taaaacacgt cgcagaatcg ttttccacat tagcttgcac
    17401 ctccatcttt caacgccact ttgtgtgtag ttgtgatcgt acgcaaatcg tagactcgac
    17461 cagtcgcatc atagtattct ttttggtttt ggatatactc ttgttctact cttcgtcgat
    17521 tttctttgtg gctttcccta ttttctacat cgatttgcgg aatagctgaa agcagacgtt
    17581 tcgtatagat atgtcgcggg tcattataaa tgtcttctct tgtaccgatt tctacaaatc
    17641 gacctttgta cataattgcg atattgtcgc acatgtgttt tactacacct aaatcatgtg
    17701 aaatgaataa atagctgagg ccgtattctt gctggattcg tttcatgaaa ttcaatactt
    17761 gcgcttgaac agatagatcc agagcagata caggttcatc ggctacgatc agttttggat
    17821 tggtagcaac agcacgggct actcctagac gttgacgttg tccgcctgaa aattcatgag
    17881 gatatttata tagcgcatct tccggcatcc caacgatatc tagcagtcct tttacctttt
    17941 tcttttcttc ttgatcgctt aaccgttcaa aatttcggat gggttcagca atgatatcca
    18001 acactcgctt tttaggattc aaactggaca ttgaatcttg gaagatcatc tgcacatcct
    18061 tgttataacc catttttttg cggttgcttc gcttcgttac atcttttcct tcatacatga
    18121 tttgtccggc tgtcaccttt tctaatccca caacggcttt tcccgtagta gattttccag
    18181 agccagactc tcccactaaa ccatatgtct ttcctttttc gataataaaa tccacaccat
    18241 ctacagcata tacatgatcc gttactcgat tgaagaatcc actgcgaata gggtaatgaa
    18301 ctttcaggtc tttgattgtg attaattctg tcatctacac ctctcctttt tcgtctctaa
    18361 agtggaaatg cttatagcag gagcaacgta ccaagtggtt cggtgcgact tcatgaagga
    18421 taggctcttc ttcatgatcg cttgctggta tccatggaat acgtggtgca aaccgacaac
    18481 ctttacgagg catgtttttc agagaaggaa cgacaccttc gatcacatgg agttctgcat
    18541 catgatttcc ttcttgtggt atagagttca gtaaagagcg tgtataagga tgcttaggat
    18601 gatcaaacag ttcttttaca cctgctactt caacgaactg tccgccatac atcactgcca
    18661 ctcgatcagc catttctgct acgactccta agtcatgagt gatcaagatg atccctgttt
    18721 ttgtttcttc ctgaaggtct ttcagtaaat caagaatctg tgcttggatg gttacatcta
    18781 gggctgtcgt cggttcatct gcaatgacga ttggaggttt gcaggcgatt gcgattgcga
    18841 tgatgacacg ttgtctcata ccgcctgata gttcatgggg atattggcgt ccaactctcg
    18901 caggattagg aatccctact tgttctagca attctagtgc acgagcttga cgctgttctg
    18961 cattcatctt tgtgtgatag gaaagtcctt ctttgatctg gtcttcgatt ctcatgagag
    19021 gatttaatgc agacaacgga tcttggaaaa tcattccgat atcatttccg cggattttat
    19081 tatagagcgt ttcgttcagg tcgattaaat tcagatcgtt atataaaatc tcacctgtaa
    19141 tttttgtgtt attaggatca agtaatccca taatcgtagt agccaaggta cttttcccgc
    19201 acccagattc cccaacgatt gctaaaatct cgtttttgtc taaagtaaat gaaacgtcat
    19261 ctactgcatc gtaatagtca tctttgagtc ggaatcctgt atgtaggttc tgaacttcta
    19321 ataattggtt atcggacaat cgaatacccc tctctgcttc ctaaatggaa gtttttctta
    19381 ataaaaaagg acattacaca gaataaagcg ttttataaca aaggttattc ttatctttgt
    19441 gtaatgaatt attaataatt atatagaaaa tcgctttact ttgcaacata atttctgttt
    19501 atcgccagaa ttatcagaaa aatgactttt acgtgtcaaa aag
//
LOCUS       DACCKU010000054        19460 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.057, whole genome shotgun sequence.
ACCESSION   DACCKU010000054 DACCKU010000000
VERSION     DACCKU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.057"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(39..1240)
                     /locus_tag="IVN13_001797"
                     /pseudo
     CDS             complement(39..1240)
                     /locus_tag="IVN13_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1357..1872
                     /locus_tag="IVN13_001798"
     CDS             1357..1872
                     /locus_tag="IVN13_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7012271.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(2071..2517)
                     /locus_tag="IVN13_001799"
     CDS             complement(2071..2517)
                     /locus_tag="IVN13_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7012272.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IVN13_001800"
     CDS             complement(2564..4042)
                     /gene="msr"
                     /locus_tag="IVN13_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7012273.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(4396..5025)
                     /locus_tag="IVN13_001801"
     CDS             complement(4396..5025)
                     /locus_tag="IVN13_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7012274.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(5009..5473)
                     /locus_tag="IVN13_001802"
     CDS             complement(5009..5473)
                     /locus_tag="IVN13_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7012275.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(5960..7228)
                     /locus_tag="IVN13_001803"
     CDS             complement(5960..7228)
                     /locus_tag="IVN13_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP7012276.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(7459..8196)
                     /locus_tag="IVN13_001804"
     CDS             complement(7459..8196)
                     /locus_tag="IVN13_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7012277.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYSDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(8263..8832)
                     /locus_tag="IVN13_001805"
     CDS             complement(8263..8832)
                     /locus_tag="IVN13_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP7012278.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            9010..9831
                     /locus_tag="IVN13_001806"
     CDS             9010..9831
                     /locus_tag="IVN13_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013711932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7012279.1"
                     /translation="MSRAAKSLHITQPTLSKQLKDLEEELGVTLFTRGSREIQLTDSG
                     RYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAISFISDSLHSLIKAHPDI
                     QFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLPAQDRWGLLIHRNHPLAK
                     QTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVGTYNLLYNASLMVDNGTF
                     GALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSPAARKFLDVLTEKYS"
     gene            10173..11045
                     /locus_tag="IVN13_001807"
     CDS             10173..11045
                     /locus_tag="IVN13_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7012280.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHTETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            11042..13000
                     /locus_tag="IVN13_001808"
     CDS             11042..13000
                     /locus_tag="IVN13_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7012281.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSDKA"
     gene            complement(13251..14102)
                     /locus_tag="IVN13_001809"
     CDS             complement(13251..14102)
                     /locus_tag="IVN13_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7012282.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            14225..14692
                     /locus_tag="IVN13_001810"
     CDS             14225..14692
                     /locus_tag="IVN13_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP7012283.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(14955..15437)
                     /locus_tag="IVN13_001811"
     CDS             complement(14955..15437)
                     /locus_tag="IVN13_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7012284.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LSESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(15537..16400)
                     /locus_tag="IVN13_001812"
     CDS             complement(15537..16400)
                     /locus_tag="IVN13_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP7012285.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            16998..17846
                     /locus_tag="IVN13_001813"
     CDS             16998..17846
                     /locus_tag="IVN13_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012286.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(18017..18358)
                     /locus_tag="IVN13_001814"
     CDS             complement(18017..18358)
                     /locus_tag="IVN13_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAP7012287.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SVEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAVEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            18444..19151
                     /locus_tag="IVN13_001815"
     CDS             18444..19151
                     /locus_tag="IVN13_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP7012288.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            19138..>19460
                     /locus_tag="IVN13_001816"
     CDS             19138..>19460
                     /locus_tag="IVN13_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP7012289.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
BASE COUNT         5829 a         4003 c         3467 g         6161 t
ORIGIN      
        1 acttgatgca ggacagcttt ttcacaacct ctgatttctt attcttcttc atttgtagca
       61 aaataattac ggtgataaat gatacaacat acactgataa gaacaatggc acctgcaaaa
      121 taaaacgcat agctttcacc aatatgttca ccaatcacac ctgcaataaa tggtgctaaa
      181 gctccaccga aaaagcggac aaaactatag ctggacgaag caacgtttcg ttgcagacct
      241 ggtatttcca tagcaatcgt cgtcaacagt gtattgataa ttccttggaa aaatcctgct
      301 aacacaatgc tgatcggtac aaggactggc atagaagttc cagctcctaa taacagcaaa
      361 caaataatga ataagacaaa tgctgctaaa attgtccgat aagtcgtaaa cttccgctca
      421 agagccggtg caacgaaaat cgaagcaata gccagaagca caccccagcc aaaaaataca
      481 aaaccgactt ctaattcaga atagccaatc aataaaaatg gcgcatacgc aagtaaagta
      541 aagaatccga aattatataa caatgcgatt agtccaactg aacgaagttt atgattgctt
      601 aacgctttga caccttccca aaggcctact tttctctttg gtttctcagt ttcaggtaaa
      661 agtaccgcta gggctataaa accgcaaaac atcaaaaatg ctaccccaaa aaatggataa
      721 cgccatgaac cactgcccaa gaccccaccg actagagggc caacagccat tccgcagcca
      781 agtgctgctt catacatcat gattgctttt tctgtgttac caactaaaat actgacgatc
      841 gttgctaaag cagtagagac aaataaagca ttcccgacac cccagccggc acgtaaccca
      901 atcagcattc cgatattccc agataatccg ccaagaccag cgaatacaac gatgataaac
      961 aatcctgtca tcaatgtttt ttcaaaccga tccgactaga taagaaacct gtaaatagca
     1021 tcaccacacc agtgactaac atgtaactag taaataacag ggtcgtttca gctggcgtag
     1081 cgtttaactg tgcagcaatc gtttttaaga ttgggtctac tagacccact cccataaaag
     1141 cgatcataca ggtagctgct acaactagcg ctgaactttt cccctttgtt tcttcatttg
     1201 tttgttcttg tatatctatt tgattctcca atatttccat ccctttctct ctattagcat
     1261 catacatgat ttattttaca tgtaaatatg cacatgtaaa acgtggaaac tttactgtga
     1321 tataataaag aaaataagac gagaggtagg atttttatga atactttagc ctatatatta
     1381 ttatgcgtgc ttttatccaa accacgttct gggtatgaat tgaagcagct gatcacaatt
     1441 ttttgggaag cccatcacag tcagatctat acaacgcttg ccaagttaga ccagcagggt
     1501 tatgtgaccg ttttggacaa agacgagcac tctcaaaaaa agatctacca cttgactgat
     1561 gaaggacgtg ctcttgtaga ggaatggatc aaagaggaaa ctcccgctcc tacgcaaaaa
     1621 gatgaatttc tagcaaaaat ctatgctttt tcgacgctcg ataaaacaac agctactgga
     1681 ctgctattca ctagagaaca acagctgaac aaaatcatgc aaaaaaatca aaaaaaattg
     1741 aatgacctct cgctagacaa gaaacaagaa tttggacgtt atgttgtgat ccagcgcaga
     1801 attatgcttt gtcagcagga actccaatgg tgtcagcaag taagagaaca aatgcagcag
     1861 ttttttgagt aagggataaa cagaaccttt ctagaaatat ctattgaaga gcaaattatc
     1921 ttcaataaaa gagcaataca gttggaaaag aaaaagcgat ccgaacgaca ggccaaaacc
     1981 tatcgttcgg atcactattt ttctagaact gcattcaaga gactatgaca aaaagcatgc
     2041 caaggccttt tttcaaataa cagtgttcca tcattcatta ctttgaattg cttttagcac
     2101 acttcttgaa agcgggccaa cgatgatcaa ctgcaacggt aaagcggcaa tcacattcat
     2161 tttccaagca ttcatataag cagagaataa ttgatcaggt attccttgtt ccatgatcac
     2221 accgaataaa gacatacatg tcaccattcc taaaaccatc aagcatgaaa tagttaagat
     2281 catttggacc ttgctttctg gatttactgg tagtttaaag gcaatttttt tcgccagcac
     2341 accgatgatg aagacatcca aaatgaaagc caccacgaat ccaggcacta gaccaatcaa
     2401 caaacttgat aaatgaagat cttgatgcag ccataaatta tacgcgctca tccccaagac
     2461 catcaaaaaa cacatcattg ttgtaaaaat tattccttct tttttatttg taggcaatct
     2521 gtaccactcc tttaaatttg tcacgaaaat catcgtagca catttaaaaa tttctcgtaa
     2581 gtactttttt tattttctgc ccttttattt cataaacttc atcagccgta cgctcgacaa
     2641 agtaagaatc atgagaagta aacaaaatcg ttcccggata tatttgcatt agcttctcta
     2701 aagcttcgat cgttgccata tcaataaaat tagtcggttc atctaacagc aacacattac
     2761 ttggcttagt aaaaagcaaa gcaatcgtca gacgggtcgc ttctccccca cttaatgtac
     2821 ataacggttt cgtcaaagtt tcctgagcaa accctaagtg attcagcaaa ctacggacaa
     2881 gcgattctga aaactctgtt tgtctcatga caaaacgaat gatggattct tcagatgtca
     2941 tttgataacc cttttgctga tatacttgaa agcttacttt cggagagaga aggattcctt
     3001 ctccttgttt gcggatatgt tctaataagg ttgtcttacc tgaaccattt tcaccgacaa
     3061 tcgcgatttt tttacctaaa gaaaactgaa aatcaccatt taccagtaat gttctcccac
     3121 ttctttccaa ttggacattt tgtcccatga tgggatagcg gctgtggagt tcaagagatt
     3181 ttggtactgg aaaacggatt tgtttcatct gctgcggctt ggttgtttct cctatttttt
     3241 ggagtcttct ctctaatgtt ttcgcattct tttggatggc tttttgtaca ctgtcttttt
     3301 gtttagagga agacaaccga tccggtctga tttgttgctt tttcttttta ctggacactt
     3361 tttccaactt ttccgcttgt tttttctttt cttggatcga tttttccaat cgttttttct
     3421 ctttcatgat cgactctgct tctcgtagct gagtcttttt ctccaattcc ttttgagtga
     3481 gataggcact ataattcccc gaaaactctc ggatttctcc gtcttttact tcccagattt
     3541 tcgatgctag ctcatctaga aaatagcgat catgactaac aaaaaccagc gtaccataat
     3601 aataacgaag ctcttcaatc agtcgatcaa tactttttct atctagatgg gttgttggct
     3661 cgtctagaag catcccttgt tcataagaag aaagaagacg tatcaactcg atctttcgtt
     3721 cttcccctcc gctccatttc cgctctgaag tttcttccag tcttaactgg aaatgactgg
     3781 ctaattccaa atttaggtcc tttgcttcag cgagataggt taattgaggc aggtagttga
     3841 aggtgatctc tgtttggatt ttcccatgat cgggaaaaag ttcaccggct attagtttga
     3901 gcaaagtcga tttaccgaca ccattttctc ctatgatccc tatacgatct tgctgataga
     3961 cacgaagaga atcaatagat agttctagct ggttgccaaa gctgacttgt agatttgtta
     4021 tatttactgc taaattttcc atatgtttta cactcctatt tcatgtaaaa caacaattat
     4081 cgatactaaa aaagagcaga aaaatctgtt tgcaaaaaaa ctgcatcaca tcttttccta
     4141 ctctgaacga aataactatg actaaatcac caaatctgtc atagctttca cctattctac
     4201 tgtcgttcga aaaaatggac atactccatc catgaaattg ttgtttatac atgtcttttg
     4261 aatttattta gttgttattc aaaagttcga aacgtaattt catcgatgca gtcatttttc
     4321 cgacctcctt ttttattagg ttaccacaca cgattcaaaa aaagcaatag ctactgaata
     4381 gtaactgaaa accttttatt cgaacgtagt gatcttttct ggtgctaaac gaacagactc
     4441 gtaaccttct tctaccgctt ttcctattga taaaataata actggaacat aacggtcttt
     4501 atccaaacca aatgcctctg ccaactgatc tgcttcgaat ccaccaattg gatttgtttc
     4561 atagccatga tcacgcgcaa ctaacatgaa ttgcatagcc gctaagctag aatcgacttt
     4621 gactacatcg ttcatttctt ctcttgaaaa actcttgtaa taaggaatga tcgcagctaa
     4681 ttgttgatct ctgacttctt ttggcatctt ccctgattcg taagcttggt tatagatttc
     4741 ttcgccatat tcgtaacatt gcatatcgcc aaaaatcatg accattgcag aagaagtatc
     4801 attttgtctc gtattgaaac ggatcaaagg acgcaagatt tcttttgctt cctcgctttc
     4861 agcgaccaca aagcgccatg gttgcatatt gacagaagaa ggtgcagtcg tcgcttcttt
     4921 gatcatttcc agcatttctt cgtgtgggat tttgtatgtt tcatcatata cgcgaacgga
     4981 tcttctacca gtcataattg ttgaaaaatc atttttttgt tccattatta ttaacctctt
     5041 tcgtttctgt tgttatattg caaagttggg tattcaattt atctaaaagt gttagaaatt
     5101 gtttcatttc tacatcgctg aaagccgggg atagcacgga acctgtatct ttatggtcca
     5161 tttcacaatg ctcgattttc ttctgggctt tttctgttag cgctacatac acttcccggt
     5221 tgttctgagg atttctctga cgcgaaacat agccatttgc ttccaatacc ttcagatgac
     5281 gagtaactgc tccttgatca atctgcaact tttcttgcaa actgctttgt agcaatggat
     5341 tttcttcttt cagtctcatc atgatctcgt atctagtaag acttataccg atattttctt
     5401 caaagatctg gacgattttt tgatcggcta atttgatttg atataaaagg tgactaatct
     5461 cttctaaaga cataatttcc tcctttctaa taattgactc gtcaacgatt gacttgtcaa
     5521 taatagaata aaaaaagaaa aaagtcaaag aatcagctgt gtaataaaaa aaacgctctc
     5581 aatagaaaag tatagagaaa agaaattttt tgtagaatga acggaaaaaa tgataaagaa
     5641 agcacaacgc taaaaccaag attattgatt cttatcaaac tatagaagat agctcttctg
     5701 cagtagaaaa ttttacaccg ccaaaaattt ccgaaatcgc cttttgcaag aatcccgtcg
     5761 aatattagaa attggatcag tatgcttaaa cagtaataaa aaagttatga caagatgttg
     5821 tcacaacctt ttttgagtaa acggtgttca aaaacttgca tctgcggtaa taagttcaac
     5881 caaacaaaaa acatgaaaat ctgtttttgt ttggctgttc ttactacttg atgcagggca
     5941 gctttttcac tacctctttt taataaccat aaaatcctat tccttcatat ctccagccca
     6001 tcggtgttag ccaatctctt tccttagcac tcaaggtata gtgatgggta ccagtttttt
     6061 catttggatt aaatagtcga taaattggct tggaaccacc agaataaaaa cttagacctt
     6121 cataacgcca accgattttt gttaaagagt tgatctcaga ttgtgccatt gtgtaatgat
     6181 ggtctccact gtaaggattg tataaacggt agacaggttg tccgctgttt ggtgcttgcc
     6241 aagcaatccc ttcatattcc cagcctacag ttggcagata atttttttct ccggcattca
     6301 atgtatataa atgttctcct gtatgacggt tatacagacg atacatcgga gaagttgaag
     6361 ttggtgctgt atcatactgt gtcagattat aggtttcgat tacattgttc aatgtagaac
     6421 cataattagg cgcagtggca taacgacctg ttagccaagc cgtagcatct ttgtaagagt
     6481 tcgtattgct tttccatgca ccggaatagt agtaaacgcc tggctgaaaa gaagtcgtct
     6541 tcaataccgt tacgttatct tgcaatgatt ctttgtacga aggatatttt ctaaaagcgg
     6601 cattcaccgt aatccattgc ccattcacat attccgaagt aggcatcgta acagattggc
     6661 cattatagct tcctttaatc ccaaatagat tgtaattagg ggcagaagcc aaagtactgt
     6721 tcccccagcc actttcaacg atggcttgag caatcatgac tgatgcatat aagtcattgg
     6781 cacttgccaa actttgtgca gttgaggcaa tactatcaat gaacgcttgt tgagaattag
     6841 cagatgagcg agcataaaaa gcatagccag tatagttttc gaattcagac aaatctgctt
     6901 gatgggtatg tgtcaatgtt tgcccatctg tcaattggtt cgtgatttct tctgcattct
     6961 ctggattgtt ctgagcttct gctgatgtac tgtcttcggt ttgtgtttgt tctgttgttt
     7021 cagttgttgg ttcttcggtt gaaattgtat tttctgagac agtagctgac tgcaaagtac
     7081 tctctgtatc gttttcaatt cctgtttctg aagaactcga tgtctgagtt gcgacagttt
     7141 ctgtcgattc ttcagctagc actgttgtgt tggcaactgc tagatttcct aaaagaataa
     7201 cgctaaataa taaattcttt gctttcatct tttttctcct ttgaattatg aaacttttcc
     7261 taaataacag tgtaactaaa aaagtaaaag caagtcaatg tccatattta aataacattt
     7321 gtttcttttt gataaatttg ctttttctac ataaaaagac gtaaatgaag aatattcttc
     7381 tccattcacg ccttaaattg atggtcgttt gaataactat ttaacaagta attctctttg
     7441 gatattatcc gttatggatt atacttcctg tgtcgtcgga cggataatca tgttactgac
     7501 agacatccgg tctggcgttt ctatcgcaaa tgctacagca gaagctatat cttcggaggt
     7561 caatccccat tctttttgcg cttcatgaag ttcaagagca gcctttttgt cgctgatggt
     7621 ttgatataat tcagtttgaa cagctcctgg agagatgatc gtagatttga tgttattttg
     7681 ccgttgctct tgacgtaatc cttccatgat tgcacggaca gcaaattttg ttccgcaata
     7741 aacggcggaa tcagaataaa caacatgtcc cgccacagaa tcagttgaaa tgatatgccc
     7801 agatttttgt ttttccatga ttggaagtac ggcggaaatt ccatttaaca cacccatgat
     7861 gttgatatca agcatatttt gccattcatc acggtgacct tcaactaatg gagcggttgg
     7921 catgattcct gcattattgt acaatacatc gattcgaccg aacttttcca tcgctaaatc
     7981 gataactgct tggacttgtg caaaatctct tacatctgct gtttgaatat atagctcagc
     8041 ttctggtaaa gattctttta ttgcttttag gcgatcttct cggcgtgcag ctataactag
     8101 tttagcccct ttttctgcta atagcttcgt tgttgcttca ccgattccgc ttgaagcgcc
     8161 catgatgaca atcacttttt ctgataaatc attcattcta ttctcctcct gttttgaaaa
     8221 atctcttgct tatactcact atctcttatt tcatcttact gttcaaataa tccgttcgac
     8281 aggtttgacg attttggcag ggattcctgc aacgatggta ttgtctggga catctttcgt
     8341 aacagttgca ttcgctgcaa caatggcatt ctcgccaatc gttacgcctg gcagaatagt
     8401 tgctcctgct ccgatccaag catttttctt gatgtggatt ggctttactg acaagcctct
     8461 tcgttctttt ggatcggtta gatggttcac cgtgatgagg ttgacacgag gaccaatcaa
     8521 tactgaatct tctaaacaga tgccacctaa gtcaacgaac atcacttgac gattgataaa
     8581 gacatttttc cctatactaa tatgtttgcc gaaatctgta taaaaaggca gcagaacagt
     8641 caccgtatca tctactttgt cactaatgat attttctagc cgtttgcgga tatctgctgg
     8701 ttcatggtac ccagtattca gcgctgctaa aggctcttcg ttttcagctt ggatctgatg
     8761 gatcacttca aagagcggat ccgttactcg aatctcttta ttgacgattt gtttttcgat
     8821 gaggcttgtc attttgctcc ctccttttat ccttacttta taacggacgg cttatcatgt
     8881 ctaatactta taaaacatac ttaactattc cttttaacta tacttagtgg tatgcttttt
     8941 taaaaacagg agggaaaaaa tggatattcg tatactgacg tatttttagc agtagctaga
     9001 aaaaaaaata tcagccgagc agcaaaatct ctgcatatca cgcaacctac tctctcaaaa
     9061 caactgaaag atttagaaga agaacttggt gtgacacttt tcactcgggg ctcccgagag
     9121 atacagttga cggattctgg tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg
     9181 gtagaaaaaa cggcgaacaa tttaacaaat gacgcaatca tctctggaga aatctacatt
     9241 ggcggaggcg aaactaaagc aatctctttc atttctgata gccttcattc gttgatcaag
     9301 gcgcatcctg atatccagtt tcatttatat agcgggaatg cagatgatat catagacaaa
     9361 ctggatagcg gattacttga ttttggactc gtaatcgaac cgacaaacaa acaaaaatac
     9421 cagtcatttc gactgccagc ccaagatcgt tggggacttt tgatacatcg aaatcatcct
     9481 ttagcaaagc aaaccgtcat ccatccgcag gaactgaaaa ctgttccttt gatcatttcc
     9541 cggcaaacgt cagtggacag tcaactagct gaatggctag gtttcagcat agatcagtta
     9601 tcgatcgtcg gaacatataa cttattgtac aatgcttctt tgatggtcga taacgggact
     9661 tttggtgcat tagctctaga tgggatcatc aatacttatg ggacggaaaa aatattcatc
     9721 ccactcgatc cgccattaca agcttcactg aatctcattt ggaaaaaaga ccagcctctt
     9781 tctcctgctg ctcgtaaatt tttagatgtc ctaacggaga aatacagtta agtagagttt
     9841 ttttccttcg ctttcagctt tttaacaacg aataagataa gcaaaagatc caacgattaa
     9901 ataacataag tttcttaatt tatttcttcc ttttatttct gataattcca gtaaattact
     9961 ataattataa aaaaagaatt gacaaaacat aactgtctat ataaaattga ttacgttaac
    10021 aataattcgt ttcatgtgac tagatcatac taggcatgaa aaatctctga ggaacatggt
    10081 ttccctagga ggtttttcgt gtctattttt gtttggccgc aatcaactgg tctagcaaac
    10141 gtgaaaacca aatcttcaaa aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa
    10201 atgcagtatt ggtagatgac ggtggacagg aaaaagtagc tattggaaaa gggattggat
    10261 ttgacagaaa aagaaatgat ttgatttttg atcgtgatgt cgaacggata tttgtcatgg
    10321 aaccagaagg tcaaatcaag ttgcaaaatt tacttaatca gattgatgaa cgctttttat
    10381 ttgcagcaga aacgatcatt gatcatacgg aaactgtttt gatggaaaag ttgaatgaac
    10441 atgtattgat cgctttgacc gaccacattg ctttttctgc ggagaacatt aataacggta
    10501 tcgtcatccg caacaaactt ttaagagaaa tcgaagtgct ttacagtgat gaattcagta
    10561 ttgctcaatg ggcagtagag tatctgacaa aagagcttgg gattccatat acttatgacg
    10621 aagcaggata tatcgcaatc catattcaca gcggacgaag cggtcggaac aataaccatc
    10681 gcagtatccg tgaagtgacg attgtatctg acatcatcca tttagtggaa tcagaattgg
    10741 aaacagatat ccattcagaa ccattttcac tgaattattc aagactggtg aatcatttac
    10801 gactgcttct acaacgaacc catgcgcaac aatatgctgt tttagacacg gaaatcgtag
    10861 aaatggtcaa acgaaaatac ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc
    10921 tgaccaaaga ataccagctc gcgatcacga aagaagaact gggttacttg gccatccata
    10981 tcgagcgttt aagaagcgca atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa
    11041 attgaaagca tatctacaac gaatgggacg ttcccttcaa cttccagttg cggtcttacc
    11101 tgctgctgct ttactagtag ggatcggaaa ctggtgggca tcttatagta acgacattgt
    11161 cgctcatttt ttacaagctg gcggtaacgc agttttaggt caattacctt tgctgtttgc
    11221 cgttgggtta gcactaggga tgtcagtgga caaatcaggt gctgctgcct tggcaggttt
    11281 ggtcgcattt gaagttccag taaatgtttt aaaaactgat tcagtagcta ctttattgaa
    11341 tatcaaacca gaatcagttg atccatcttt tgctcagatc agtaacgtat ttattggtat
    11401 tattgccgga ttgattgcgg cagctcttta taatcgtttc cacgaagcaa aattaccgat
    11461 ggctttatct ttctttagcg gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt
    11521 gctgatcagt gcagtgctac tgctcgtatg gcctccggtt tactctgctt tagtttcttt
    11581 tgctaaattc attttgagtt taggctgggg tggtgcaggt ctttatggct tcttcaaccg
    11641 tttactgatc cctacaggat tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc
    11701 aggaatcaat gatattggta aatttttggc ttctgaagga acaaaaggcg taacaggaat
    11761 gtatcaagct ggatttttcc caatcatgat gttcgggctt ccagcaggag catatgctat
    11821 ttacagaaat gcacgtccag aaaacaaagc aaaaacagct tccttgatgc tagcagcagc
    11881 ttttgcttca ttctttactg gtgtgacaga accacttgaa ttttcattta tgttcgttgc
    11941 ttggccatta tatgtcttgc atgcaatttt cactgggatt tctttagcag tgagtgcatt
    12001 cttccattgg acagcaggat ttgcctttag tgccggattt gtcgattact tcctatcttt
    12061 gaaaaatccg attgcgaatc agccattgat gttgatcgtc caaggattag taacagcagt
    12121 gatttactac tttggcttta actttgctat caaaaaattc catctgatga ctcctggccg
    12181 cgaggaagca gatctttcag atgacgatac tgcaacgact aattcagaca ataaatatgc
    12241 ggcacaagca agcaaaatct acgctgcatt aggcggagcc gacaatgtaa cttcgatcga
    12301 taactgtact acacgccttc gtttgcaagt caaagataca ggcctgatcg atcaaaataa
    12361 aatcaaagcc actggcgtac ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat
    12421 cgtaggtaca gaagtacaat tcgttgcaga tgagatggca aaactacatg gtggtgctgc
    12481 agcacgacct gcacaaacaa acaccgtggt taaaacagaa acatttgccg gtgaaacgat
    12541 cgaacaagat atttacgcta tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga
    12601 tgatgtattc gcggaaaaaa tgatgggtga cggctatgct gtattaccag aaaatggaga
    12661 aatcttctct ccaatcgctg gtacgatcac aaacattttc ccaacgaaac atgctgtcgg
    12721 aatccaaaca gatgctggta tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt
    12781 aaaaggcgaa ccattcactt tatatgtaga agaaggtcaa aaagtcgcaa gaggtcaatt
    12841 gattgcttta gttgatcttg ccgctattca atctgcaggc aaaaacacgg atatggtcgt
    12901 tgtattcaca aatggcgaca aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa
    12961 agcaaatgat aaaatcggct ctgtttccga caaagcctga gccatcgtct tccccagact
    13021 tttttatcga taaacaaaca ggagctgaga caaaaatctt gtctcagctc ctttttccga
    13081 ataaaaacgg cgttctcctg ccaactccgc ggtaataagt ccagccaaac gaaacatatg
    13141 aagagctatt ttttatttga ctcttcttat tacttgatgc agaacagctt tttcacaaaa
    13201 tttttcttca tactcaaaac taatcggctt tttcacaacc tctttataca ttattcctga
    13261 tccaatattt cttttaatgc tgcttcaaga gaagttaatg gttttcccaa tactttttct
    13321 aaatcatcag aggaaatagc taactgatcc tttttaatat catgttggat cgctaaaaac
    13381 atgtcagctg cttcttgagg gaatccggct tcttttagat tttcgacaaa cgctgcatca
    13441 cttgcttcaa taatttctaa ctctttacct gtcgcacgct ctaatgcctt ggctaattct
    13501 tcatagtcag ttagattgcc ggataattct aagatttctg gagagtcact ttctactagt
    13561 acttttgctg cagcttctgc atattcacgt ttcaatgccc aacctgtttt tccatttcct
    13621 gcagcataaa cgaattgacc agtttttaaa gcctgcccaa tgataggtaa ttcattctct
    13681 acataccaat tattccgtaa aaatgtatgg gaaatacctg aatccttaat cattttttca
    13741 gtgaattggt gatctggcgc taatacactg atcgagtgat cggcatctgc aaaacttgta
    13801 taagcgatat aagatacccc agctgcttgt gctgccttca ctacattctt atgttcggct
    13861 tgacgatttc ctggaacgcc agagacaaaa agcaatcgat ctattccttt caacgcttct
    13921 ttcattgagt ctaaatcatc ataatcacca atacgtatat taaatccagc ttctttcagt
    13981 gcttctcctt tttcttcact acgtactagt acatagattt cattttgtgg aaccatcttt
    14041 tttaagaatt ccagcgcata accaccaaaa cctccagttg ctcctgttac taaataatgc
    14101 atatttgcca ctccctattc tttttatttg ttaagattta cagctgtaca tttataaatt
    14161 acacctttat cataaagagt gtggtaagat aagtcaagca aaaaaaatga aaggatgaca
    14221 actgatgaag tattctctgc aatttagtga tgcaatccat gtacttgcct atattgaaat
    14281 gtttcaagat acccgatcac tttctagtga gatgattgcc agtagtatcg aaacaaatgc
    14341 agcaaatgta cgtaaaatca tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac
    14401 cggaaaaccg cagccttccc ttgccaaaag tccagatcag atttctttac tagatgtcta
    14461 tcgcagcatc gaaggtaata cacagcttat ccaagtcgat ccaaaaacaa atccaaattg
    14521 cgtcgttggt gcgaatatcc aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa
    14581 ggcagaagaa gaaatggcta agatcacact tgatagtctg atacataaaa tagccgtttt
    14641 ggaaaaagaa aaaagaccag aaaacacagg cctcgtagag agatttttat aattctctct
    14701 actgtgcctg catttttctg atcggaatcg tctacataaa ttcttttatt ctccggggga
    14761 caaaagcctt gtcatgcccc tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat
    14821 gacgtcttga taaacggtgt tcaagtagct gaccctcggc attagttctg aaaaatgcaa
    14881 aatatggaaa gctatttccc atttttcctc tctaatgctc aggtcaaagc gctactttca
    14941 cgacctctat attatcatgg tttattgact accggtatcc agatttctcc catatatttc
    15001 ccgttggtaa ctcccatttc gactacagca tttggacccc caacataggc gtaatcttct
    15061 acttctctta aaacatgccc aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc
    15121 gattctgata aagcgcttac aacaagatac gttccttttg ggaatgggat gattcgcgta
    15181 gtgtttggaa catcctgact cgtttgaaca ccgacatagt acatcatttt cccgttatag
    15241 ggttcgttga ctgtaaagaa ataatgattt tcagcagctt gtttcaatgc atctattgtt
    15301 ccattaccga taactttttg ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc
    15361 gtattgctac tcttcaattc cacaccaata cccgtcaccg taaaatctgg tttttcttcg
    15421 atcctatact ttgccatttc tcccaccctt tctattatat taagttcatc ttacataaaa
    15481 ttaaagggaa atgacacgat tacgctcttt tttctaggga tatttttatt ttttaactat
    15541 ttttttgctt tcactgcaat ccaaatctca ctgtaaacat tactgaaatc ctctccccat
    15601 tttgtaaaag agatctcggg agcttcgact aactcataat ctgaagaagg aagccattct
    15661 gcatagattt tcccccaagt atcttgtaaa gtttggggaa atggcccttt attaggaaaa
    15721 atggcccaag tgtgttcttc tatagcaagc atttctaaat cagcgtacgg attttctttt
    15781 gtcgtagcaa agccaatcat gtgcatcaaa ctgccctttt cttccaagcg gtctccatca
    15841 aatccataag aaacgttgat tacttgattt ggatataaat ctcctaactt atgcatctct
    15901 tccctttgag tctccgtgat cgtctgcgct aacttttgta tttcttgatt gactccttca
    15961 aatttgacag gaactttttt cgccacacct gccacataaa actgttcttt cttttcgata
    16021 cgaaattcca tcgattttcc tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat
    16081 ttttgtatgc cgtatctgct gatttctgaa ggcagatatc ctgaccaatc acgaaaagct
    16141 cttgaaaaac cttcgactgt ttcatatcca tatctaaacg ccgtttctgt cactcttgat
    16201 ccatctagaa gatcgcgatt agccaaagcc aatctacggt ttttgatata gtccattagc
    16261 gacatccctg caataaacgt aaagatgcgt tttaattgat attcagagat tcctgtattt
    16321 tcggctatta ttttatagga aagtccttct gtcaaatgtt cttcgatgta atcgatcaat
    16381 tggttcagat ctttaagcat atttatcttc ctccttgctt gcctttttat catattatca
    16441 aagaaagtca attacttgat gaatcaggaa ccaatttagt cagatgtaga aaatgttgta
    16501 tggatctaaa gaaaattctc aagcagcgtt tcgtaacgga taattggtat tatctgccta
    16561 tctatgtttt acctttagaa aaataaattt tggggaagac agattctaag taaacaaagc
    16621 ggtaaaaata tcttttattc ccttagttaa tccgcttact acctactgta caaagcaaat
    16681 tgttgttcaa actgctgatc agaacgctgc ttggattttt ctttgcgata catcatacct
    16741 cttttatcaa ttggaacaat tatttatttc taacaaaatc acttcatttt tgtcagataa
    16801 ataaaaaaca aaaaaagttc catctttaaa acacgctatt cttttacttt ttctcacatt
    16861 ctctaagaaa agaaaaggag aatgaatttt tgacaattcg agcaaaagtg attatcaaat
    16921 atgtaaagtc agctactttt ttcttaaacg tttctttata ctaaatataa ataagtgttt
    16981 catgaaagga gaactagatg aataattggc ttgagtattt tcctgaaaac gttttggaac
    17041 gcggttacag ctatcatctg cacggttttg tccgtcattt gaactataca agcaagtact
    17101 tgtccgcaac agtttctggc acggaagatt ataaggtagt gatcacttgg gatgaaaaga
    17161 ccaacatgac ttgtgattgt ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg
    17221 ctgtcttatt cgcttacgaa gaacgtccaa taaaaaaatc caactactca ctttccgaac
    17281 tctcgtccct tgtttcctcc gctagttcct ctcttgtacg ggagctttta acagagattc
    17341 ttatagaaca tccgcacttt atcgaacgct tcaaagttaa gatgccattt catgcaataa
    17401 attattcaga taaattaaca actatcatcc ataaatacga tcatattatc aagaaaaaca
    17461 aaaacaggaa aacagcgaag ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg
    17521 aatcgcttat tcagcaaaat gcctatcttc ccgcattcga attgatcaat gaagtgattg
    17581 ctactctcga aacattctat tgggagcctg aggatgaacg aacccttctt ctgatagagg
    17641 actgttatta tttatggaaa gaattattag cagaagcacc tcatgcagaa aaaaggcaaa
    17701 tgttctcttg gttcgtctgc caagtcgatc atactgacgc ctcttattca aaacgctact
    17761 ccataaaaat cctgaaagaa gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa
    17821 tagataaaaa actaaagaaa cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa
    17881 cgataacaac catcctaaat ccacaagaaa tacagcagac cttgctttaa aaacacgaat
    17941 catgcaaagg atgaagtaaa gaagacttaa cgcgtaccca atattagtca tttgactatt
    18001 aggtacgcgt tttgttttat aagtcatgac aaatttcctt cgttgctgga tagattttcc
    18061 ggcattgttg atagatttta tgatactttt ctacattttg taagagaggt tccactgcat
    18121 ctgtatattg gataaaattc tgacaacaac tttcaggtgt gtcgaaccac cctaacccta
    18181 gtgcagcaat cattgttgcg cctaatgcag gtccttcctc cacacttaaa caatagaccg
    18241 ttgcatcgaa aatatcagct tggatttgta gccatgcaag atttttcgcg cctcctccca
    18301 ccgaaacaat acgtgtaaat ctgcgacccg ctagttcttt acgtttcaat cccaccattg
    18361 acaaatgctc attaaaaatt tagaatattc acaagtttaa cgacaattat gaaaatttat
    18421 gtcgtgctaa aggagttttg ttattggatg aaaagttaga tataaaaact gcgatcaaag
    18481 acacgttacc taccgttttc ggttatatcg gtattggact tgcatttggt atcgttggga
    18541 aagctgccgg atttcatcca ctagtcgtca cgttgatgtc cctgctggtc tatgctggtt
    18601 ctgcccaatt tatcacagtc agcatgcttg ctagtcacag cccattgctt tccatcgttt
    18661 tctcgacctt tctagtcaat tcccgaatga ttctgatgag tatgacgatt gctccttatt
    18721 tcaagaaaaa tcggctgctt caaaatctgc tgattggcac attgctgaca gatgaaagtt
    18781 tcgcactggg aatgaataaa ttgaactata ctggacaaaa gttaaacttt cgttggatga
    18841 atacagctaa ttggatttct tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt
    18901 tagggaattt tattactgat ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt
    18961 ttatcgggtt actttatctt caagtcattt ctgatagaaa cacctctaaa cgattacagt
    19021 tgatcctgat tggtctcact ttgattctgg tatatgtcgg actgattttt atcccaagca
    19081 acttagtgat tgtagttgta acactgattg ggtgcggatt gggggtgtgg atcaaacatg
    19141 ccttcttttg agtttacatt gctgactatt ttaggttgta ctttagcaac ttggctttct
    19201 agggtcctac catttgtctt actgaaaaaa ttcgatttac cgcaaccgtt gcttgagtat
    19261 ttaagttttg tcccgatcgt cattatgtct gctttatggt tcagcagtct atttacccaa
    19321 aatatcggac atctgccgca gatcaatctc gaaaatgctt tggcttcagt tcctacactt
    19381 ttagctgcta ttctttcaaa aagcttattg gtgatcgtct tggcaggaat tctttccctt
    19441 tcgttgattc gcttattata
//
LOCUS       DACCKU010000055        19140 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.058, whole genome shotgun sequence.
ACCESSION   DACCKU010000055 DACCKU010000000
VERSION     DACCKU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 19140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19140
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.058"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(144..1013)
                     /locus_tag="IVN13_001817"
     CDS             complement(144..1013)
                     /locus_tag="IVN13_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7012290.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(1033..2154)
                     /locus_tag="IVN13_001818"
     CDS             complement(1033..2154)
                     /locus_tag="IVN13_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7012291.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(2156..2551)
                     /locus_tag="IVN13_001819"
     CDS             complement(2156..2551)
                     /locus_tag="IVN13_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7012292.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(2570..3367)
                     /locus_tag="IVN13_001820"
     CDS             complement(2570..3367)
                     /locus_tag="IVN13_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7012293.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(3369..4157)
                     /locus_tag="IVN13_001821"
     CDS             complement(3369..4157)
                     /locus_tag="IVN13_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012294.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(4171..4647)
                     /locus_tag="IVN13_001822"
     CDS             complement(4171..4647)
                     /locus_tag="IVN13_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012295.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(4719..7376)
                     /locus_tag="IVN13_001823"
     CDS             complement(4719..7376)
                     /locus_tag="IVN13_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7012296.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(7395..7799)
                     /locus_tag="IVN13_001824"
     CDS             complement(7395..7799)
                     /locus_tag="IVN13_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP7012297.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(8031..8288)
                     /locus_tag="IVN13_001825"
     CDS             complement(8031..8288)
                     /locus_tag="IVN13_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP7012298.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(8413..9345)
                     /locus_tag="IVN13_001826"
     CDS             complement(8413..9345)
                     /locus_tag="IVN13_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012299.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            complement(9751..10506)
                     /locus_tag="IVN13_001827"
     CDS             complement(9751..10506)
                     /locus_tag="IVN13_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7012300.1"
                     /translation="MDFEQNVRLNFDLLSDNEKDMVFFIRTHKAQVVHMSIVELGEQL
                     LSSKSSVLRLAKKLGFRGYSEMKYSLEQSIVQSVIPPTDLIALLKDEINRTFQLADQT
                     NFQPILEQLKAAKTVVVYATGFTQNNFSKDFVNDLILSGRPAMLISGETNFEMLSHTL
                     TKDDFVIVTSLSGETPSIKSTIKNLNMNRIPLCGVTELGKNFLSEHSDFLLYYETREL
                     PSNLIEGSRSMIGLNILLSILSRKYREFILFDE"
     gene            complement(10613..12283)
                     /locus_tag="IVN13_001828"
     CDS             complement(10613..12283)
                     /locus_tag="IVN13_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7012301.1"
                     /translation="MNEWWKSSVVYQVYPQSFKDSNNDGIGDLMGIVEKLPYLAKLGV
                     DVIWLTPIFDSPLIDNGYDIRDYEKISENYGTLEDFKRLLEQAHQLNIRVILDLVVNH
                     TSNQHAWFIESQKNKENPYSDYYIWKDPKEDGSAPTNWGSTFGGSAWEYVESRKQYYL
                     HCFAKEQPDLNWENPKVRQSIYKMMKKWFDLGIDGFRMDVISLISKHPDYPDAPNSMP
                     YTKSYYVGASNGPRVHEFLQEMNREVLSKYDSLTVGETPNTNSRQAQLYTFPERKELN
                     MVFHFDHMHLDYGKYGKFSDVRFKLSDLRATLSEWQEALSDGWNSLYWSNHDQPRAVT
                     RFGDENEYRIESSKMLGTLLHMMKGTPFIFQGEEIGMKNVPFSSIDEYQDIETNYFYK
                     KMKEEGESDEYIKRAIYLKSRDNARTPMPWDHSKGYGFSNSDPWISFSPDNQQINVED
                     ALKNPNSVFYHYQKLISLRKNYPIIQHGTYKLINENDSAVFGYIREYKNELLVVICSF
                     SKESVPYELPKSLQGRKGVLLLSNYEQSPSELEDKFILRPYEALVYHI"
     gene            complement(12287..12706)
                     /locus_tag="IVN13_001829"
     CDS             complement(12287..12706)
                     /locus_tag="IVN13_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HAP7012302.1"
                     /translation="MKKIIFASHHNLAVGLKDTVQYILPNVGEITAIAGYIDNSSIEE
                     KINEVMVRDNEDTDILIFTDMLGGSVNQAFIKYINQPNVYLITGMNLPVVLTILLNTL
                     NKRLTDNHIREAIQEAQDQLVYVNDYLKNIVVDEEDE"
     gene            complement(12719..13537)
                     /locus_tag="IVN13_001830"
     CDS             complement(12719..13537)
                     /locus_tag="IVN13_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7012303.1"
                     /translation="MILMNNEGNKLSKKDIRRAAFRYMFMACNIFNYENQQGPAVVYG
                     LEKALRKIYPNDEDYRASLNNHFKYFNTTTWMANLLLGASLAMEEKDGVSSLDAVQSF
                     KTGMMGPLAGVGDTLVWVLYPTIMGSIAAYMGLEGNPTGAIIWLCLNLFFLFFRFKLF
                     SIGYTSGLKLVTALGSKLSIFTESASIMGLTVVGALIPSVVKMNVAVVFQSGKVKLPI
                     QTEILDKIMPALLPAVLTIIVYKLIASKKMTVIQIIFSVIIFALILSFFGILKV"
     gene            complement(13521..14321)
                     /locus_tag="IVN13_001831"
     CDS             complement(13521..14321)
                     /locus_tag="IVN13_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018664850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012304.1"
                     /translation="MEIIQGILIIILAVWVVLDQQGLVITTWFPIMIALFAGLIMGDM
                     TTAMVIGGTFQLMALGVANIGGSSVPNWGLAALVGIYVAIRTTNNIEEAKAVALAVGV
                     PVGMLGIQLDVLAKILNTYVTHAAQKAANKMDFKKMNQILWLGPVIFGLSTAIPTALC
                     VVFGDRIVRLILDFVPKWFTDGLSIAGSMLPVVGIALLLQVMPVKKYMTMLLIGFVLS
                     AYLNMPILGISIVGFALAYYFFTTEIKKAETASPVAANIVDEGDDFDE"
     gene            complement(14336..14812)
                     /locus_tag="IVN13_001832"
     CDS             complement(14336..14812)
                     /locus_tag="IVN13_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012305.1"
                     /translation="MGLVLARIDQRLIHGIVVTQWAGATKAKRLMVVDDQVSKDDTQK
                     AAMRMSKPAGTGMSIIDTDTAIINFKLGKYDSHNVLMIVKEPSTLVKLAENGIDIPKV
                     NIGIMFDGEGKQTVKKMVSVDEQEIADLKKLEAMGIPVTFHFVPSDPEEPLDVRIK"
     gene            complement(15207..15686)
                     /gene="rlmH"
                     /locus_tag="IVN13_001833"
     CDS             complement(15207..15686)
                     /gene="rlmH"
                     /locus_tag="IVN13_001833"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP7012306.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            16114..17400
                     /locus_tag="IVN13_001834"
     CDS             16114..17400
                     /locus_tag="IVN13_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7012307.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTTSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSGF
                     GGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKDG
                     TKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANS
                     VTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKI
                     ASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQE
                     QILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKV
                     GDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(17439..18044)
                     /locus_tag="IVN13_001835"
     CDS             complement(17439..18044)
                     /locus_tag="IVN13_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP7012308.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLGADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWSGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(18249..18713)
                     /locus_tag="IVN13_001836"
     CDS             complement(18249..18713)
                     /locus_tag="IVN13_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7012309.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(18735..19055)
                     /locus_tag="IVN13_001837"
     CDS             complement(18735..19055)
                     /locus_tag="IVN13_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP7012310.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
BASE COUNT         5922 a         3881 c         2876 g         6461 t
ORIGIN      
        1 ggataatttt tctcttaaaa aatatcatta tttgtggtat ggagctgggt atcatgagtg
       61 aatgatattg ctgaatacaa atagtccgac cacattaagt tgatcggact tcataagcat
      121 tttattgact aatacttttt tgcttaagaa ttgattctaa catcttttgc taaagttaag
      181 catcctatag ttcctgcatt atctgctaac ttaggtgtga caatgtattc ttcgatattt
      241 ggcgtatcga catagccgtt aattagttct aaaaaacgtc tttgtacttt ctttttcaga
      301 tgttcttgtt gcatgacacc accaccaaaa attataatat ctggtgaaag catcagggtc
      361 gtattacagg cacattgtgc aatataatcc gcctcgattt cccagaacgg atcttctata
      421 gaaagatctt gtcccttggt tcccgtacgc atctcgatag cgggtccaga tgccaaccct
      481 tcgagacaat catgatgaaa aggacaagta ccagagaaag tatctcctga atgtctctta
      541 actaacgtat gccccatttc tggatggcta aacccttcaa caaaaatccc atcttgtata
      601 gctcctccac caattccagt tccaacagtg taataaacaa cactggataa accttttcca
      661 tgacctgaaa catactcccc gtaacaagca gcattcacat cggttgtcca ggcaattgga
      721 atttcgaaat gcttattcaa cgttccaaca aaatcaaaat ttttccaggc agtttttgga
      781 gtagaggtga tatgtccata ggtctttgat tcacgatgga tatcaatcgg tccaaatgac
      841 cctactccaa tgctttctaa ttgttcttta tagtgattaa agaaatcaat taccaaactc
      901 attgtttcat tcggtgtagt tgtaggaaaa ctcactcttt ctaaaacctt taaatcatca
      961 ttgccaatgg cacaaacaaa tttagtccca cctgcttcta ttgagccata catttcttac
     1021 tccaccgtcc ttttatctag gaattcgttc ttccctgtct aatagttttt cgaaaaactc
     1081 atgtggttcc ttttggtacc aataggcaac agaagaaata tcgtcttgac gctcataaag
     1141 cttgatgtca tcatttccta gggtttggaa agtaacacgt aaattttctt tgaaactaat
     1201 ggggtcgggt aaatgccagc ggtacaaccc atgcatcggt aaagcatcat cgccaaaacc
     1261 atgaactgga tttggattag gttttccagc actaaaataa tcccgggtgt gatctctttt
     1321 agattgaaaa ggatagccca taaatagcgt attaaaattt tgagcatatg ccctttctcc
     1381 aaagttcttt ttatggaaag cccaagcgcc gccaaaataa tcttctgctc ctgtagatgt
     1441 tattgtaggg aaatcttcat ctccatctat ataaaactta aattctcctt ccccccacca
     1501 gtatctttcc aatgcgcaca aagcaaagta ggttcctaca taatacccag tgcctttgat
     1561 tccatcaata agggtgtaat cttttccttt tgtcgtgatg ggttcacggt tccatttagc
     1621 atgaaaatac atcgcatcat ccggtacttc agtcaatgca taattaaatg tataaaaaat
     1681 gtgcttaata aactcaggat gttcattcgt taaagtaatc ttagcttttt ttcgaaatgg
     1741 catctcaaaa tagcaattca tccctgatgt tggattcact acgattggta aggaattcac
     1801 ttcgcatctt gtcccaaacc cattacaaaa aaaatctcct aatggacttt ctacagaagg
     1861 agtggattca tcatcccaat aaatacgcaa aattaaatca cgtaagacga aactcccagc
     1921 gattgttttg tctgtcactg tcatccaaaa atgacgtaaa attccagggc cttccatttc
     1981 tgctaaagta attgtctctc cagcatttat cggaacagaa ggtgaccctt ttctcccaat
     2041 tcctagatga ctcgctgtca ttgccgcttt ccctttttca ccatttttat tttctggtga
     2101 aatagttcta ctttcctctc ccttgtggag ccatatatta tttaaacaat tcattttatt
     2161 cgtcctctat cctttcttca cttttttgat tattcatata gatgatttgt tgttgtgcct
     2221 ccgcaataat tgctccaata tctaaattat catttcctaa ctgcaaagcg attgacattg
     2281 ccaagggaag attcattcct gcaattacgt gcactctgtc agaaatatag gtaaaaaact
     2341 tttgatttac acttcctcca gccatgtcag ttaaaacaat tatttcgtct gtagtatcca
     2401 ctaactcttt aatttgttca tctatatcat caatttcttc gacatatgca cataagctac
     2461 taatttgatt atgcatacct gttaaaaact ctagtgtctc tttaaaaccc tcagctagta
     2521 ttgaatggct agcaatgatc aatcttctca taattcaaat ccttttcctt tacgctaata
     2581 ttccaaatgc agccataacc atcgcaaaaa caataacgag tagaatcaaa atggtcatac
     2641 tgattttttt cttactgagt agataatatg ctgcagcagt aataattact ggaattaatg
     2701 ttggtaaaat tttgtccaac attggttgga ttttcatact aacttcacca aaagtgaatt
     2761 taagtggcgt tgaaacactg ataactgaag caattaatga tccaactact gttagcccca
     2821 atactgaagc agcttcagtt aaataattaa tcttttcacc gtattcagta acaatcttca
     2881 ttccctgctt ataaccaaat tcaaagcctc tcattcggaa aaagaagatt accatgatta
     2941 aaatgaacca taaaaagatt cctacaggat tattttctaa agccatataa gccgcaattg
     3001 atcccatgat ggttgggatc atcgtcatca aaagagagtc cccaatacca gcaattggac
     3061 ccatcgtacc aactttaaag tcattaatcg catcttttgc ttctaatcct tgcttttcct
     3121 ccattgcaac acaagcacct aaaataatgg ctgccagcca aggctgtgta ttgtaatact
     3181 gaaagtgatt atttaaagct tcgatgtatt catcatcatc tttgtataat tttcttaaaa
     3241 taggtgacaa tgcgtaagtc acagatgcac cttgctgtgt ttgataatta aaggtacaaa
     3301 ctgacatcaa ccaacgatag ttcgctttcc taagatcctt tttcgttact tgatattttt
     3361 cgctcatttt attcatcctc cccaataaac ccaacatcag aagctcccgc agaagcagtg
     3421 atttgtgcat ctttttcagc tctatgatac acattaaatg ctaaagcgaa tcctatgatc
     3481 gccgctccaa taataggtac cttcaaaaaa gcggctaaga aaaatccaac aagtaaataa
     3541 ctaatgtatc gacctgtagg cataaagcgt aacaacatcg caataccaac agcaggcaac
     3601 atttttccgg ctaatgttag cccatccata aaccaattag gtaaaacatc caaaacgtaa
     3661 ttaacagcag actgtcctaa agtaacggca ataaatactg gaattgctgc agataaacca
     3721 aagaaaattg gccccaacca caaaacacta ttcattttct taaattctcg tttattcgca
     3781 tattcctgtg atttgcgaac aacaaaacca tttaagattt tgacaattac atctagttgc
     3841 acccctaaca tacctacagg tagtcctatt gctaatccgg cttctactcc aagctggcta
     3901 ccaaatgccg ttgcaataat tgcgcacgtt ccatagtccg gtacagatga gccaccaaaa
     3961 ccggcaactc ctaatgacat caattgaact gtaccgccaa ctatcaaacc tgtttttata
     4021 tcacccatca aaattcctgt gaataatccc caaaaaactg cgtaatttac aaagatcatc
     4081 ggaatcccat aataatcaac atgtttaata aatgctaata tagtcagcat aataatttga
     4141 aaaatagtaa aatgcattat ccttactcct ttatcctaat tttgattgaa tttcctgtgc
     4201 cttatccgcc ggaacaaatt gtgtaacaat ttctacacca gaatccaaca attttttata
     4261 cgtgctgata ttttcatctg atgcataggc tctagaagat agctgtaatg cattatcttt
     4321 aacaacagta ccgccaacaa ttagtcggtc aattctaata ccgttatcta ctaaatgaag
     4381 gattgtttcc ggctctttca cgatgacaaa tactttttcc tgatcgtatt tccccttttt
     4441 aaaattcgca atcgctgttt cttcagtgat aattgataca gccattccag caggtctagc
     4501 taaacgcata cttgattttt ggatgtcgtt atttgcgaca gaatcatcga tcaccatcac
     4561 tctgtttgct ttagacattg gtgcccattg cgtgacaata atcccatgca ataaacggtt
     4621 atcaactctc gacataacaa tactcataaa tacttcctcc tttatctttc attttcttac
     4681 aagcaattac catgccaaaa caaaaagaac ttgataaatc aagctctttt tacataatca
     4741 taaacgtatt taacctcatg aatcgggatg tcgacactat aataatgttc aacgacactg
     4801 aaagattgct taaaatgtgt ctgaaatttt tgaaattcct cactcgtttc attgaaataa
     4861 atttcatctt ccaaagtatt ttgttttact aatcgttcaa ttaagcaact aatgtgaaca
     4921 tataatccga aacacgtatt attatcaaat gaaattccta gatcattttg taagatgtat
     4981 atagcatctg aaacccgttc taataattta gtcggattta atatggtcaa ttgctccatt
     5041 aaattcgtta gactaaaatt cttcagaata ttattgttca aaactttcag ttcattatca
     5101 ttaaagtgtc tattcaattg attggagaat ttacttatat tcattcctaa aatcatatct
     5161 tcaattgaaa agaattctac atcttctaga tgtggattta gtgttccaac aataaaaaga
     5221 atattgttct ctttacgaag ctccgataaa tagttttcat tcactaaaga gtaatagtca
     5281 cacgtttcta tgacaagatc agtatccctt ggaaagctat cttcaagtat cttttttaat
     5341 ttaaacgaag ttcctaatcc gcttgcacaa gaacagacaa tcatatcctt cttaatattt
     5401 gatgattcat aaatataatc cacattttga ttttctttta cttgttcaag aatctgttta
     5461 agcggtaaat ctaataatat ctgatttgca acatcaatag ccattcttgt tgaaacattg
     5521 tttagtagtc cgaatttttt ttgagcgtct agttccaaat ttagataaat tttctccagt
     5581 gaacccatat caacaagtaa aataatttct tcatatccgg tacactcttg taagtatcta
     5641 ttaattttgt ctgcaatctc actacttgaa gtcattagtg gcatatcaat ggcgtcgtat
     5701 atatatctct caacgatcct attagcagta cttgctatac aggttgccgt tagatctcca
     5761 tgagccaaaa tcagtgctaa acgtttttgc tgaaattctt tactacacat taacttaaat
     5821 aaaataacca aaacattttt ttgaattttt gttaaataaa tatcccctat ttgaataaaa
     5881 cgattgacta attgaacttc tcgactacac caactattaa tatcattgat gaaggaatat
     5941 aatttatcgt ccgatgttaa ttggatttga taaatccaag aattcaaaaa gatagacgtt
     6001 atttgaagat aataatagtc aaattctatt tgaaaatctc tctctatttc cttaacatac
     6061 tgtctaaatc gaatctcttg ataggtggga tatgttaaag ctgaattatt ttggatatca
     6121 acattctttt gtctaaaata tctatcatat ttctcaaaaa gctgctgctt atcagtgtct
     6181 ttcttccacg tttgataaag atactctata agttcgttga tattaaaaac ttcgtctact
     6241 ttttccaaat catccatttg aatatatttt aattcattct gtcttttcat aaccttcatg
     6301 ccgttgaaat tcacccattg tggtaaatga tacatctgaa tataaagttc ttccaaatca
     6361 tctaagaaat aaaaggcatt ggcacaggtc aattgaatga tatttttcaa attgccaaca
     6421 ttttcactaa agcgatagtt taataaaaaa tctaataatt gttgagatat cagtattttc
     6481 tttgctagtc tttcttcttc tgatttaaaa aatatcttga ttaactgttt tctttcataa
     6541 agagaacgac tgtctaatgt tggaagtgtt agctttacag gtattcttct caacaaagtt
     6601 gataatagtt tatcctcagg tggttctgat gttgcaaata taatccagca acttgatgtg
     6661 taccagtttt cattatcacc catccgatga aatttcctcg tatccataaa ctgaaaaagt
     6721 ttctcctgac attcattgct caatgcatga atttcatcta aaaataagac tccattatta
     6781 gctaattctg ctaaaccctt tcttccatct tcggctcctg tatatgcacc ttttacatgc
     6841 ccaaaaaaat tggacaagaa aagttctgga ttattggcat attcactaca attaactgaa
     6901 atgaaagatg ccttctcttc tatataatta ttggccaaca aatccttata cacataattt
     6961 gctaaatagc tttttccagt tccagtctcg ccatgaagta aaatgggtaa gccatttggc
     7021 ggatagtaga ttgctgcttt aatttgagca attacatttc gcagacttcc ttgacttccg
     7081 ataacccttt ctattccaac cctaaaagag ctatcatacc atttttccgc ttcctctgaa
     7141 gagctaaaaa tttgttgatt tggaactttc tccggtaaag aaaagaagat tactggacga
     7201 gtattgattt ttccaaattc tccatctttt acgaattcat tcaaatattg agagacaata
     7261 ttacgactaa catttaattt ttcagatatt ttctgtgctg tattgttccc ccatttattt
     7321 ttaatctcag agttctctat gacataattg acaatgcgtt cttttaattc ttccatcaca
     7381 gcacctcctc gacactattc gaattttgcc gacagtattt ttccataaat atgacttgct
     7441 aaaaaggctt tgtttagatc attcaattgg aacgttgctt gttttgcttg atccgctaag
     7501 acgttgaaaa gatactctgg cgaaatcgca taccccaaaa tatcaacgat tgttgcaatc
     7561 agatcttctt ttgattcttc atctgaaagt aattcacgat acaacatcgt ttgtttgctt
     7621 actgtgtcat attcctctaa ggattcatct gctaaaagaa ccacttgttc caacaaacgg
     7681 tcggataaat atttatagaa aatcagcccc aataaataat ttttatattc tgacgcatcc
     7741 attttactac ggagattatc tgccgcacta aataacttct gatttaattc tgcacccaaa
     7801 aagatacact cctatctctt atttaaagtt gctatatacc ctcttatttt agcatactca
     7861 gttttagaaa aagcaaaaaa ataaagaaac ttattcttga taacaagcta cgtgacgtat
     7921 atccctaaca caaaaatagc catatccttt ggtttggata cggctatttg atattactca
     7981 ttcaattatc aaatcacagt aggtgtgaat ctcatcggga tcggcaatat ctacttctga
     8041 ttaggatcaa aggtgatttt ctcaataaag gtgtgataca aagctttaac ttgcttcttc
     8101 tcactctgga taagaaagca atccaaacta gtcaatatct gttggacatc tttgacttga
     8161 atcggttccc cttgttgttg aaagatccct aagatttcat tctctcgaat ttgtaactgt
     8221 cttcgttttt cagtcaattc atttaaacga tcttttaaca tcggaaacaa ttcgggacta
     8281 tcttccaaag cagcttcctg tttttgctct aatagttaga ttttgcgtac cattttcttc
     8341 gtttattttc cttcgattgc tgattttaat atagtctaaa ggaaatttat atttattgta
     8401 ttctttataa cttcaagaga acttcgtttg agtattactg tattttttta attccataag
     8461 aaaattagtt attacctgcc acctaatcag caaatctgaa cctgagtcaa tataattaat
     8521 attattttcc atctccaaca ttgtaagtac tgttggtgtt aatataggga ctttgtacaa
     8581 ttgtattttt ttgattttat catctgcaca aaggagattt tttaaatcta atccttcttt
     8641 tatttcaaaa gttgaatttt ttaaatctat aagagtaaaa ccaattcctt catactgaat
     8701 ttctgaaaat cgaatttttc tcccagttga tagatcaagg ggcataaaac cttttattac
     8761 attgtgagtg tcaccatcat ttacaatgtc attaggaatt ttgattttag aattctgtgg
     8821 agcacctatt ggaagataat aattcattat aattttgtag tcttcatgag gaaataaagt
     8881 acttttagga ttgctaagat attctcgtac cgaatctaag aatggcttat ccattgaatc
     8941 ttcatcccca tttaaactaa aaaacattgc tacaattgct tttgcaactc taagtgaatt
     9001 cattttcttt attgaatgtg ttaaatcaga cttaccattc tttaaacttg tattgttata
     9061 aaggaaattt ttattgtctt ctgcaaatct aatatattcc tcaacatact cactaccaaa
     9121 aaaactatta catttactac ataacgtata ataaacattt ccttgaggtt tccttttaat
     9181 ccccttcgga aaatcacctt ttgtaaaatc gctcttaagg aaatcatcta aattaccagt
     9241 tctggtcttt ttacctccag tattccctga ttttttaggt ggtacatgtt ctttagtaag
     9301 ttcagtgtat ttttcacata aaatacactt acccatcttt ttcatatcta tccccactct
     9361 ttttaattct tatatacaat tatatttcaa atattcatta ataaatatcc ctatttatga
     9421 taaggaccta cataactttc cccctagata ttacgaatat ccagggggtg ttattgcatg
     9481 cgtaacagcc tatgctaatt tacaacatag atcgaagtgg cttagctcta aaaaataagc
     9541 cgcatctttg tcagatacga ctgtttagca ttgctcattc aattagttga tgattttttc
     9601 atcaaatttc agatatagtt gaatatttcc tttgttcagc ttatcaaatg tcactttatc
     9661 aatgaatgtt cgatagattt ccttgttctc actcttgaaa cgattggcta acaacttttc
     9721 aacacttgct cctttgtttc gaaatggagg ctactcatca aaaagaataa attcacggta
     9781 ctttctagag agtattgata gcaaaatatt taatccaatc attgaacgac ttccttcaat
     9841 taaattgctt ggaagctctc ttgtctcata atataacaaa aagtcggaat gctcgctgag
     9901 gaaatttttt cccagctcag taactccgca aagtggaatt cggttcatat tcaaattttt
     9961 gattgttgat ttgatgcttg gtgtctcacc gcttagagac gtaacgatca caaaatcatc
    10021 ttttgtcaaa gtatggctta acatttcaaa gtttgtttca cctgaaatta acatggctgg
    10081 tcgtcccgat aaaatcaaat cgtttacaaa gtcttttgaa aagttatttt gcgtaaagcc
    10141 tgtcgcatag acaactactg tttttgccgc ttttagctgt tctaatatcg gttggaaatt
    10201 tgtttggtcc gctaattgaa aagttcggtt gatttcatct ttcaatagtg cgataagatc
    10261 tgtcggtgga ataaccgatt gaacgatgct ctgttcaaga gaatatttca tttcagaata
    10321 cccacgaaat cccaattttt ttgctaaacg taagacagaa cttttagaag acaataattg
    10381 ttctcccaat tctacaatgg acatgtgaac tacttgagct ttatgcgtac gaataaaaaa
    10441 aaccatatct ttttcattat ctgagagcaa atcaaagttt agtcgaacat tttgttcaaa
    10501 atccatttcc tcacctctca tatatacaga aggaatttcg gataaaactt gcgctgtttt
    10561 atccgaaatt ctttcttatt gcccaaattc taacacagtt tttacgtgct tgctatatat
    10621 gataaacaag tgcttcgtaa ggtcgtaata taaacttatc ttccaattca gatggtgatt
    10681 gctcataatt tgatagaaga agaacccctt ttcgaccttg caatgattta ggtaattcat
    10741 agggaacaga ctcttttgaa aaagaacaga tcaccactaa tagttcattt ttatactcac
    10801 gtatataacc aaacactgct gaatcattct cattgatcaa tttatacgta ccatgctgaa
    10861 tgattggata gtttttacgc agtgaaatca gtttttgata atgataaaaa actgaatttg
    10921 gattttttaa agcatcttct acattgattt gttgattatc tggcgaaaaa gagatccaag
    10981 gatcactgtt cgagaatcca tacccttttg aatgatccca aggcattggt gttcgtgcgt
    11041 tatcacgaga tttcaaataa atagcacgtt tgatgtactc atcactctcg ccttcttctt
    11101 tcatcttttt ataaaaatag tttgtttcaa tgtcttgata ctcatcaatt gaagaaaatg
    11161 gaacattttt cattccaatt tcttcccctt gaaaaataaa tggggttcct ttcatcatgt
    11221 gaagtagtgt tcctaacatc ttactagact ctatccgata ttcgttttca tcaccaaatc
    11281 tagtaacagc tcgcggttga tcatggttac tccagtaaag agaattccaa ccatctgaaa
    11341 gcgcttcttg ccactcactt aacgtagctc ttaaatcgct aagcttaaac cttacatctg
    11401 aaaactttcc atactttcca taatcaagat gcatatgatc aaaatgaaaa accatattta
    11461 attctttacg ctcaggaaac gtataaagct gtgcctgtct gctatttgtg ttcggtgttt
    11521 caccgacagt taagctgtca tatttactca aaacttctcg attcatctct tgtaaaaatt
    11581 catgcactcg cggaccgttg gatgctccga cataataaga ctttgtatag ggcatgctat
    11641 ttggagcatc aggataatca ggatgcttgc taatcaagct gataacatcc attcggaatc
    11701 catcaattcc aagatcaaac cattttttca tcattttata aatagactga cgaactttcg
    11761 ggttctccca gtttaaatct ggttgttctt ttgcaaaaca atgtaagtaa tactgctttc
    11821 tactctcaac atactcccaa gccgacccgc caaatgtcga tccccaattc gtaggtgcac
    11881 tcccatcttc tttgggatct ttccaaatat aataatcaga atatggattt tctttatttt
    11941 tttgtgattc tataaaccaa gcgtgctgat tacttgtatg attgacgaca aggtctaaaa
    12001 taactcgaat attcagttga tgcgcctgtt ccaataatct cttaaaatct tctaacgttc
    12061 cataattttc tgaaattttc tcatagtctc gaatatcata tccgttatca attagcggag
    12121 agtcaaaaat tggagttagc cagataacat caacccctaa ctttgctaaa taaggtaatt
    12181 tttcaacgat ccccataaga tctccgatac catcattgtt tgaatccttg aaactttgag
    12241 gatacacttg gtagacaaca ctacttttcc accattcatt catccgttac tcatcctcct
    12301 catcaacgac tatatttttt aagtaatcat tgacataaac caattggtct tgtgcttctt
    12361 ggattgcctc acggatgtgg ttgtctgtca atcttttgtt taatgtattc aataaaattg
    12421 tcaataccac aggtagattc atcccggtaa tcagatagac attaggttga ttgatatatt
    12481 ttataaatgc ttgattgact gaaccaccta gcatatcagt aaaaatcaat atatcagtat
    12541 cctcattgtc tcttaccatt acttcgttaa tcttttcttc tatagatgag ttatcaatat
    12601 aacctgcgat tgcagttatt tcaccaacat tcggtaaaat atattggaca gtatctttca
    12661 atcctacagc taaattatga tgactagcaa aaataatttt tttcattctt tattctcctt
    12721 aaacctttaa aataccgaaa aatgatagaa tcaatgcgaa tatgatcact gaaaaaataa
    12781 tttgaatgac cgtcattttt tttgaagcta tcaatttata aacaataatt gttaaaactg
    12841 caggtaagag tgctggcatg attttatcaa gaatttctgt ttgaataggt aattttactt
    12901 ttccagattg aaaaaccact gcaacattca tttttacaac agatggaatc aaagctccaa
    12961 cgactgtcaa acccataatt gaagcagatt ctgtaaatat cgataattta cttcctaaag
    13021 cagtcaccaa ctttaaccct gaagtatacc caatcgaaaa aagtttaaat cggaagaaca
    13081 aaaagaatag attcaaacat aaccaaataa tggcaccagt cggatttcct tctagaccca
    13141 tataagctgc aatcgagccc ataatcgttg gataaaggac ccaaaccaac gtgtctccca
    13201 caccagctaa aggacccatc atcccagtct taaaactttg aactgcatct aatgaactga
    13261 ctccatcttt ttcttccatt gctaagctgg cacctaataa taaattcgcc atccaagtcg
    13321 ttgtattgaa atatttaaaa tggttattca gagaagctct gtagtcttca tcatttggat
    13381 aaatttttct tagtgctttt tcaagtccat aaacaactgc cggaccttgt tgattttcgt
    13441 aattgaagat attacatgcc ataaacatat agcgaaatgc tgctctacgt atatctttct
    13501 ttgataattt atttccttca ttattcatca aaatcatctc cttcatcgac tatattggca
    13561 gctacaggtg atgccgtctc tgcttttttt atttctgtcg tgaaaaagta atacgctaaa
    13621 gcaaagccga cgattgaaat tcccaaaata ggcatgttta aataggcgga aagtacaaaa
    13681 ccaatcaaga gcatcgtcat atattttttc accggcataa cttgtagaag taatgctatt
    13741 ccgactacag gcaacatgct tccagcaatc gataatccat ctgtaaacca cttaggcaca
    13801 aaatctaaaa tcaatcgaac aattctatca ccaaaaacaa cacacaatgc agttggaatg
    13861 gctgtagata atccgaaaat cactggtcct aaccacaaaa tctgattcat tttcttaaaa
    13921 tccattttat ttgctgcctt ttgtgccgca tgagtaacat atgtgttcaa tattttagcc
    13981 aacacatcta attgaattcc cagcatccca acaggaacac ctactgctaa tgcaacagct
    14041 tttgcttctt caatattatt tgtggtacgg atggctacgt agatacctac aagagcagct
    14101 aaaccccagt taggaacgga cgatccccca atattcgcta cacccaaagc cattaattga
    14161 aacgtaccgc caataaccat tgcggttgtc atatctccca taatcagccc tgcaaacaaa
    14221 gcaatcatga tgggaaacca tgttgtaatg accaatcctt gttgatctaa tacaacccac
    14281 actgctaaaa taataattaa aattccttgg ataatttcca ttccaattat ctcctttatt
    14341 ttattctcac atctaatggt tcttcaggat ctgaaggaac aaaatgaaaa gtaactggta
    14401 ttcccatagc ctctaatttt tttaaatcag caatttcttg ttcatcaact gaaaccattt
    14461 tttttactgt ttgtttccct tcaccatcaa acatgatccc gatattcact tttggtatat
    14521 cgataccgtt ctcagctagt tttactaatg tactcggttc ttttacgatc ataagcacat
    14581 tatgactgtc atacttgcca agtttgaaat taataatagc tgtatctgta tcaataattg
    14641 acatccccgt tccagctggc ttactcattc gcattgctgc tttttgtgtg tcatccttac
    14701 tgacttgatc atctacgacc atcaatcgtt ttgcttttgt cgcacctgcc cactgtgtta
    14761 ccacaatacc gtgaatcaat cgttgatcga ttcttgctaa tactaatccc atatctatcg
    14821 tctccttttt tgttatcgtt tacatcccaa agtatatcta cgccttgtta ctttaacaat
    14881 agttttagaa gtatcaggga aacagtcgtt atcttaggaa atagtttctt tattaagaga
    14941 aatttcccac aaaaaaatag cccaaccatc gattagatga ctgagctccc taaatttacg
    15001 ctatctttta ttactattta cttctttttt atcgcaatcc aaatttcgcc atcacaatcc
    15061 gtcaactctt ttgagaaatc agtaaaggag agttctgatg cttgaaccaa ctcatattct
    15121 gaagttggca accactccgc gtatattgac ccactgacat ccctaaaaaa ggtgcgaggt
    15181 ggagaatatt tttgatacaa ctatccttat ttatgatatg gagcccccgc attgatccgg
    15241 aaggcacggt agatttgctc gacaaggatc agacgcatca attgatgtgg atacgtcatt
    15301 tttccaaaag agatctgagc attgcttctt ttcatgacgg cttcgctgag tcctaaagag
    15361 ccgccgataa caaaaacaag gtggcttttt ccttggatac ctaattgatc gatttgttta
    15421 gcaaaagatt cacttgtagg atttttccct tcaattgcta aggcatagac gtattcttgt
    15481 tctttgattt tcgctaagat ccgttcccct tctttttctt ttacttgtct catttgcgct
    15541 tcgcttaaat tctccggggc tttttcgtca ggaacttcga tcaattcgat ttttgcatag
    15601 gcattcagcc gtttgacata ctcattgata ccttggatca ggtatttttc ttttaatttt
    15661 cctactgaga taattttgat attcatgttt tctctccttt ggtcttcacg ctcactttgt
    15721 ttattataat caattttttc gtctatatgt ttattttggc tatttttgtt aaaaagattt
    15781 ccccaacagt tatgcacaac atccacaggt ttatacacag aaaacagtgt tattttacct
    15841 tatttcatca cattcattag gcaaacatac gttttataag aatttatcca ctttatacac
    15901 aggctgtgga tatgttttat cccttatttt tccaatccta tccgcgtagt tttacacagt
    15961 atctgtggaa aaagaaaatc aaatattttc atctgaaaac aatgattttt cctaaagttt
    16021 ttcataaaaa gttcacagtc tcaaagtaga ctaaagtcaa caaataagac tatactaaca
    16081 gtagataaat aaatgtaatt ggaggaggaa cacatggata gaaaaaatgt gacaccaaag
    16141 atgaaaaaaa ataaaaataa tagcctctgg cgtaagttag gtctggggct tgttggaggg
    16201 atcgttggag gactactgac agctggcatt ttttatgcag cgatgggcac tggcaacaat
    16261 agttctacta caagtggcaa tcaaaattct gctggtgaaa cagttgtgga aaacgtcaaa
    16321 gtcaatgtag attctgatat caccaaagcg gtagataaag ttcaagacgc tgtcgtttct
    16381 gttatcaact tacaaagcca aaaccaatca agcggttttg gcggactatt cgggcagcaa
    16441 gaagaaagta gcagctcaga tgatagtaat ttggaagctt atagtgaagg aagtggcgtg
    16501 atctacaaaa aagatggaaa tactgcttat gtagtcacaa acaaccacgt agtagatgga
    16561 cagcaaggtc tagaagtctt gatgaaagac ggtacgaagg taaaagctga attagttgga
    16621 acagatgctt attcagattt agcggtgttg aagatcaatt cagataaagt cgaaacggtt
    16681 gcctcttttg gtgactcaag tgctctgaag gttggtgaac ctgcgattgc gattggttcc
    16741 ccattaggat ctgaatatgc gaattctgtc acttcaggaa tcatttcttc cttgaaccga
    16801 caagtaacaa gtacaaatga atcgaacgaa acagtcaata tcaatgcgat ccaaacagat
    16861 gcagcaatca accctggaaa ttctggtggt cctttagtga atattgaagg acaagttatc
    16921 ggtatcaact caagtaagat cgcaagtact tctgaatcta cttctaacgt aagcgtagaa
    16981 ggaatgggat ttgctattcc aagtaacgat gtagtgaata tcatcaatca actggaaaaa
    17041 gacggaaaag tgacacgtcc agctctaggt atcacaatgg tcgacttatc tgccgtttct
    17101 actcagcaac aagaacaaat cttgaagatt ccttcatctg taactaacgg tgtcatcgtg
    17161 acaaatgtcc aaacagctac accggctgaa aaagctggat tgaaacaata cgacgtcatt
    17221 actaagatcg atgacaaaga agtttcttct ggtgtagaac ttcaatctgc tttgtataag
    17281 aaaaaagtgg gagattctgt caaaatcact tactatgaag gctcgaaaga aaaaacaact
    17341 actgttgaat tgacgatcga tcaatcggca ttaaaacaaa atcaaaatag tgaaaattaa
    17401 taaaaaagag gttgtgacaa agacttgtca caacctcttt attttggaaa tgcttctccg
    17461 actacggcat cctcaggcaa tactaagatt cctttttttg ctggagcatt tggcaaatgc
    17521 aattcacgag cggaacagat cattccatag ctatctaccc ctcgcaaagt tcctgaccag
    17581 atcatcattc catctggcat catagctcct ggttttgcaa cgacaacttt ttgtccagct
    17641 tcgatattgg gtgcaccgca aacgatttgc aatactgtac cattgtctac ttctgtctca
    17701 gtaatcgata aatggtcaga atcaggatgt gggacgcatg tttttacgta cccgacaacg
    17761 atcttcggtt catgatcagc tccaagctgt tcttcgaatc ctgcttcttt gatcaatcga
    17821 ttcaatttcg ctaattcctc atctgttaag cttacttggc cgttcccttc gattgttaga
    17881 tgatcggaga tatggaaaaa gttccaagca acaacttgac cttgttcatc tttgatccga
    17941 gcaacattcc atttgcgttc aacggtgttt tctgttcctt tatcgtcggc tatgataacc
    18001 atcaaggtgt ctccgacgtg tgctttgtta taagcaaaaa tcatgttctt tcctccatta
    18061 tcctttgctg agatttatct taacaaaaaa ttgcattcac tgcatgagat ttttttcatg
    18121 ctttatttcc gaagaagcct cttctggaac accatttatt cgtaacaagg gactgtgaca
    18181 agaattttgt cacagtccct ttactgtttt agttaattga ttaaaaaggt cgtacaagtt
    18241 ccacttgctt aattacttgg ctgttcttct tcgtcagaaa cgatcaagac atcacaaggt
    18301 gaatgacgta tcacgtagct ggctacactt cctgtcataa agcgttcgac tgcatttaga
    18361 cctgactgcc caaccatgat tagatcgatc tcatattttt ttggcaattt gactgccatg
    18421 gattcacgaa cagaaccata tgcaataatt ccttctatat ttgtgtaatc aacagatttc
    18481 ccatattctt tacattcttc gatcatttcc ttggctgttt cagtttgctg atcgactaga
    18541 ttttgatcaa taggtgcata gcccataaag ttataaaatt gctgatcgat tacgtttgct
    18601 acatatactt tcccattatt acggcgagcc acttctaccg cttttttgaa agcagcaagg
    18661 gcttgttcac taccgtcgat acctactaaa atattcttat attcttgatt catcttgacc
    18721 cctcctaaca gatgtcagcc tttcgttaca ctttctagga acgattcgat ttcctgtttt
    18781 gttttacgat ctttattcac tagacgacct aattcttttc cgtcttggat cacgacaaaa
    18841 cttgggatac caaaaatgtt ccattcagcg gctacatcta tgtacttgtc acggtctact
    18901 tcaataaatt gccaatcaga aaagtcattt tcaatttcag gcatttgcgg tttgataaaa
    18961 cggcagtctc cgcaccagcc ggcagtaaag aaaaatacac tctttccagt ttctacgtag
    19021 cctgctaatt cttctaaact atttgggata atcattcttt gtcctctact ttctgtttat
    19081 gtatttttca attaaattat acacctttct ttcgaactaa caaacattga tcctcttttt
//
LOCUS       DACCKU010000056        18513 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.059, whole genome shotgun sequence.
ACCESSION   DACCKU010000056 DACCKU010000000
VERSION     DACCKU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.059"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(360..926)
                     /locus_tag="IVN13_001838"
     CDS             complement(360..926)
                     /locus_tag="IVN13_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP7012311.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(926..1882)
                     /locus_tag="IVN13_001839"
     CDS             complement(926..1882)
                     /locus_tag="IVN13_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP7012312.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1882..2538)
                     /locus_tag="IVN13_001840"
     CDS             complement(1882..2538)
                     /locus_tag="IVN13_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP7012313.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2908..4926
                     /locus_tag="IVN13_001841"
     CDS             2908..4926
                     /locus_tag="IVN13_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7012314.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5353..7767
                     /locus_tag="IVN13_001842"
     CDS             5353..7767
                     /locus_tag="IVN13_001842"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP7012315.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8115..9668
                     /locus_tag="IVN13_001843"
     CDS             8115..9668
                     /locus_tag="IVN13_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP7012316.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10049..11833)
                     /locus_tag="IVN13_001844"
     CDS             complement(10049..11833)
                     /locus_tag="IVN13_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012317.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11848..13569)
                     /locus_tag="IVN13_001845"
     CDS             complement(11848..13569)
                     /locus_tag="IVN13_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012318.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13669..14430
                     /locus_tag="IVN13_001846"
     CDS             13669..14430
                     /locus_tag="IVN13_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7012319.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15397..15665
                     /locus_tag="IVN13_001847"
                     /pseudo
     CDS             15397..15665
                     /locus_tag="IVN13_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16337..>18513
                     /locus_tag="IVN13_001848"
     CDS             16337..>18513
                     /locus_tag="IVN13_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012320.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         6083 a         3289 c         3727 g         5414 t
ORIGIN      
        1 aatttgactg atattaagtt agctattgat atatgaatga catacaaaaa tataaaatct
       61 tttaaaaatg cttatatatc aatgtttttg ccattctttt atatatctgt tatatgtcaa
      121 aaaaatgtgt tcaatttcaa tttgtatttg aataaagagg ttgtgaaaaa gctgtcctgc
      181 atcaagtaat aagaacagcc aatcaaaaac agcttctcat gtttttgatt tggttggact
      241 tattaccgca gatataagct tttgagcacc gtgaataaag aggttatgaa atcaacgctc
      301 taacctgagt attagagaaa aaaacgcaaa acatatcatt tatgttttgc gttttttatt
      361 tatctttttt tctcaataca atataaaata ggcggctggt tcttttggtt gataaaccga
      421 taattcagca cattatatgt ttcctgtggc agtttggaac aaaaagaatc aaccatatcc
      481 agttcttttt ctccaccttc atgaccatag tagacgacaa ggatcaatcg tccgcgaggc
      541 acaagtcgtt tcaatatttc ttccatagct gtgcgtgtag tctctggcat agtgatgatt
      601 gctttatcac tttttgggag ataccctaga ttgaaaatcc ctgctttgag attttgttgt
      661 tcagctatca cttctcctaa cgtttcgtgg ccttgtagaa acaatgagac acgttcttct
      721 aactgacgct gttctagcct ttcacgagta gcatcgattg cttgttgctg gatatcaaaa
      781 ctatagacat gaccagtttt tccgatgtgt tctgctagaa aaaccgtatc gtggccattg
      841 cccatcgttg catctacgac gtgatcccct ggttgcaaga tttcttgcag taatgtatgg
      901 ctgaaatgca aagcagtttg caacatcaga cgctcacctt tttccgtaca tcatattttc
      961 cttggaagct atctctgcga agaagttctt catcaattgc attcaagact tcccattttt
     1021 tcaagctcca cattggtcca atcaaggaat cccacggcgc atcccctgtc aaacgatgga
     1081 tgatgatctc ttttggtatc atttccagct ggtcgcaaat gacattgaca tagtcaggac
     1141 ggctcattaa ttgaaggcga ccttcatgat agtcccgcaa cattctcgtt ttgcgcatca
     1201 gatgcatcaa atggagcttg attccttgga tatctgaatc taaaatggtg cgtctgacat
     1261 tttctttcat catttctaaa ctttcgcctg gtaatccgtt gatcaaatgc gtacaaacac
     1321 ggatattatg tttgcgtaat ttagccacgc catctaaata agtttgataa tcatgtgctc
     1381 ggttgatcag ttttgaagtt tcttcaaaag ttgtttgcaa tccaagttct acccataaat
     1441 acatccgctc gttcagttca gcaaggtaat caactacttc atctggcaga caatccggtc
     1501 gtgtaccgac tgataatcct acgacacctg gtaaattgac tacttgctca aatcgttctt
     1561 tgattatttc aactggcgca tgggtgtttg taaaattttg gaaataaacg atgtattgtt
     1621 ggacatgagg ccatttttga tgcatcatgt cgatttcttt ctggaattgg attggaagag
     1681 ggtcttcagg tgcaacgatc atgtctcccg aaccagatac actgcaaaaa gtacaaccgc
     1741 catgggcaac agtcccatct cgattcggac agtcaaatcc tccatcgatc ggcaccttga
     1801 aaattttccc gccaaactca tttcgtaaag cgtaattcca agaatgatat cttttatttc
     1861 cttctgaata aggaaaagtc attaaaagtc cttctttctg taagagttat ctatttgttt
     1921 tcttttttct ctatacactg gataagtcag gcacagccaa gaaagactga gtaagtagcc
     1981 gccgatgata tcagtaggaa aatgaacgcc cagataaatg cggcttatcc cgacacttac
     2041 tataataaac atcatcagca attgaagcaa tgtgcgtaag gttctttttt gaacaaaagc
     2101 tggaagcaga aatagtaaag aaccgtaaag aatcatactg cctgctgcat gtccactagg
     2161 gaaactgtaa cttgtttcaa ctactaagtg cgccaatgaa ggacgctctc gcatgaaaaa
     2221 tagtttgatc accgggttga tgattccagc aattacaata aatccagaaa cgaaccaaaa
     2281 tgtttctgcc ctttttttct tataccacat ccataaagcc gcacaagcaa ataaaatgat
     2341 gacggtaaga ggattaccga atttagtcat aaagagttgg tagctgttca gatcaggata
     2401 agaagctctg acaatgcctg taaaccactg atcgaaacca gaaagccacg acagatggaa
     2461 ctttacgatc cagcccaaaa tgaaaaacag gacaagaaag aagctaccag ccacctgaaa
     2521 tgatgtttta tatttcatac aattcctcct ttgaagcaat gacttattat agcatttttg
     2581 gtccagcgaa aaaaacggta aagagataaa accaatgtat gattcctctt tgaataacca
     2641 atattcttat aaaaaaaata actgtaatcg gaaagacatt ttttggtaat gcaaatgaaa
     2701 tgttagggaa ataaaatcag tgtttttcga aatcctttta aacaagaacg tagacgtagg
     2761 aagaacttga aaatgaacag aagttgttca ttacggtaaa ttacaatatg attacaaaaa
     2821 acaacaaagc tcttaaataa cttttaataa atattaagag aagttataat tattaataat
     2881 agataaattg aagttggagg atagaagatg gaaaacattg caagaaaaga acggagacgt
     2941 ttagaacaga ccaaacgttt tcgaaaagca aaacgagggg cagcaatcgt gggaactgcg
     3001 atggtaggct gttctgtcgc tgccccatta gtacagccgg ttcctgttca agcggatgaa
     3061 acacctgttc agttcggcgc aagaatcaat acagcggcat ttatcacaga aattgctact
     3121 tatgctcaac caatcgctca agccaacgat ctatatgcat cagtgatgat cgcacaagca
     3181 gttgtagaaa gcggttgggg cagtagtgcg ttatctcaag caccttatta taatcttttt
     3241 gggataaaag gcagctatca aggacaaacc gtctacatgg atacgcttga atttttaaat
     3301 aatcaatggg taaataaaaa ggagcctttc cgacaatatc cttcttttgc tgaatctttc
     3361 aatgacaatg cctatgtact tagaaatact tcatttggca acggttatta ctatgccggt
     3421 acttggaaga gcaacacgaa atcctataca gatgcgacag cttgtttgac agggcgttat
     3481 gcgactgatc ctggttacgc aggcaaatta aataatatca tcgcaactta cggattgacc
     3541 caatatgata caccagctag cggaaacgca ggcggtggag cgactacagg tggcaataat
     3601 actggaagtg caggtagtac tggcggttca agcaattctg gtaatacaaa ctcaggttca
     3661 gcaacttcga ccacttatac tgtccaatct ggtgattctg tttggggaat ctcaaataaa
     3721 ttcggtatca caatggatca attgatccaa tggaacaaca tccaaaacaa tttcatttat
     3781 ccaggacaaa aactgacaat caaaggtagc caggaaaatg gctcttcgac gaataattcc
     3841 ggtaacaata ccaactcttc aggaaatgct ggtacatcaa acggaggaca aacaactggt
     3901 gcgaaataca ctgttcaatc tggtgattct gtttggaaga tctcaaatga tcacggaatt
     3961 acgatggctc aattgattga atggaacaat atcaagaaca atttcgttta tccaggacaa
     4021 cagctaatcg tatcaaatgg cggttcttct aatgctggag caaatactgg ctcaaatact
     4081 gggaatacaa gtggctctag ttcgtccagc tcaagttctt ctaatacagc ttcaggaacg
     4141 aaatatacag ttaaagccgg agactctgtt tggagcgtct caaataaata cggaattacg
     4201 atggctcagc taatccaatg gaacaatatc caaaataact ttatctaccc aggacaacaa
     4261 ctgactgttt caaacagtgg atcaacaaac agcacgtcag gtaatacagg ttcgtcaacc
     4321 aatacaaata acagcagttc agttaatact ggcagcagtt cgtctcaagg cacttatacc
     4381 gtgaaagcag gtgaatctgt ttggagcgtg gcaaataaaa acggcatcac gatggatcag
     4441 ttaatcgaat ggaataatat caaaaacaac ttcatttatc caggacagca attgatcgtt
     4501 aaaggtggaa cttcggcaaa tacaaataca ggttcaacta cttctgcagc gaaaccaaat
     4561 acaccgaata cgtctgctac gacaagtaca tcatccactg gtaatacgat gtataccgtg
     4621 aaagcgggcg agtcagtttg gagcgtagcg aacaaacatc atatcacgat ggatcagtta
     4681 atcgaatgga ataatatcaa aaataatttc atctatccag gacagcaagt gattgtgaag
     4741 aaaggttcat ctcaatcaac aaaccaacaa tcagttactg gttcgaaaac ctatactgtg
     4801 aaatctggag aatctgtttg gagtgttgct gacagtcatg gaattacaat ggctcaacta
     4861 atcgaatgga acaatattaa gaacaacttt atttatccag gtcaaacgtt gattgtagct
     4921 aaataaaata gctgttcgaa gaaataacga ttgaattcca caaaaatatt tgttactata
     4981 aggctactta aatatgacaa cacttgaaaa ggaagtagta aaaagactga agtttcagag
     5041 agcgcatggc tggtgagaat gtgcacggat gcttttgaac tcgcctttga gttgcccttg
     5101 ctgaataccc gagtaagcaa gcggcggtga cggccgttac aacgttcgag gccttttcac
     5161 taaggtaaaa ttaggtggta ccgcgtagat tacgtcctat aaaaagagca atctttttat
     5221 aggatttttt tatttctttt gtgaagtttg agaaaacaaa gcaaaaggaa ataaaaataa
     5281 acagattctg ccggtttttt cgttatattt ataatagaaa caagtgtttt caatagaaca
     5341 ggaggacagc aaatgagtta caatcataaa gaaattgaga aaaaatggca aaaatactgg
     5401 gcaaaaacga atactttcaa cacccatgat gatcctgaaa agccaaaatt ttacgcactg
     5461 gatatgtttc cctatccatc cggtcaaggg ctacatgtag ggcatcctga aggttatacg
     5521 gcaacggata tcctttcacg tgtgaaaaga agtcaaggat acaacgtgct gcatccaatg
     5581 gggtgggatg cattcggtct gcctgctgaa caatatgcgc tagatacagg aaacgatcca
     5641 gctgagttta cccaaaaaaa tatcgaaaca ttccgacgcc aaatcaattc tttaggattt
     5701 agttatgact ggaatcgcga agtgaataca actgatccgg agtattataa atggacacaa
     5761 tggatcttta cgaagcttta tgaaaaaggt ctagcttatg aagcagaagt agcggtaaac
     5821 tgggtacctg aattaggaac agttatttca aatgaagaag tgatcgatgg gaaaagtgaa
     5881 cgtggtggct atgatgtagt cagaaaacca atgcgccaat ggatgctgaa aatcactgct
     5941 tatgctgatc gcttattgga tgatctagaa ttagttgatt ggccagaaag cattaaagaa
     6001 atgcaaagaa attggatcgg tcgttcagta ggagcaaata ttgaatttaa agtggctggt
     6061 acggataaat cctataccgt atttacgaca cgaccagata ctctattcgg tgcaacttac
     6121 tcggttcttg cacctgaact agatcttgtt agagaaatta cgacccctga acaaaaagct
     6181 gctgtagaag cttatatcga agagacagct aaaaaatcag atctaaagag aacggatctt
     6241 gcaaaagaaa aaacaggagt atttactggt gcttatgcaa tcaatccagt taatggcaag
     6301 gaaatcccaa tctggatcgc cgattatgta ttatcttcat acggcacagg agccatcatg
     6361 gctgttccag cacatgatga acgtgacttt gaattcgctc aaactttcgg actagaaatt
     6421 cttccagtta tcgaaggtgg agatgttgaa aaagctgctt acacagaaga tggtacgcac
     6481 atcaactctg aatttctaga cggcatgaac aaacaagaag caatcgataa aatgaatgaa
     6541 tggctagaag aaaatggcgt agggaaaaaa gaagtcagct atcgcttacg tgactggcta
     6601 ttctcccgtc aacgttactg gggtgaacca atcccaatca tccattggga ggacggaaca
     6661 gtcacagctg tcccagaaga agagctacca ttacggttgc ctaagacaaa aaacatcaag
     6721 ccaagcggta ctggagaatc tccattagca aatatcgagg agtgggtcaa tgtcgttgat
     6781 cctgtaacag gtaaaaaagg ccgtcgcgaa acgaatacaa tgccacaatg ggcaggaagc
     6841 tcatggtatt acctgcgtta tatcgaccct cataacaaaa aagaactagc agattatgaa
     6901 aagttgaagc gttggctacc agttgacatc tacattggtg gagcagaaca tgcggtactt
     6961 catttactat atgcacgatt ctggcataaa ttcttatacg atatcggtgt tgttcctaca
     7021 aaagaaccat tccaaaaact atacaatcaa ggaatgatct taggcgaaaa caacgaaaaa
     7081 atgtcaaaat ctcgtggtaa tgtagtcaat cctgatgatg tggtcgaaac ttacggtgca
     7141 gatacactgc gcatgtacga aatgttcatg gggccattag atgcttcgat tgcttggagc
     7201 gaaaatggct tagaaggaag ccgtaaattc ttggatcgtg tatggcgttt gatcgttgat
     7261 gagaacaata aaatgcgtga ccgtatcact acattgaatg atggcaagct tgataaagtg
     7321 tatcatcaaa cagtgaaaaa agtgacagaa gactatgaaa atcttcattt caatacagca
     7381 atctcccaat tgatggtctt tatcaatgaa gcctataaag tggacgctct gccttatgaa
     7441 tatatcgaag gatttgttca actactcgca ccgattgctc ctcacatggg tgaagaacta
     7501 tgggcaatcc ttggaaatga tggtggtatt tcttacgcac catggccaac atacgatgaa
     7561 gcagcacttg ttgaagacga agtagaagtt gttttccaag taaacggtaa agttcgtgcg
     7621 aaaagcaatg ttccccgaga cttaggaaaa gatgagctag aaaaagcagc attagccaat
     7681 gaaatagtgc aagaatatat tgaaggcaaa acagtcagaa aagttattgc ggtaccaaac
     7741 aagttagtca atatcgttgc taattaattg gctctttgtc aataaggact gatgagttgt
     7801 gcaaaatcaa atctgggcca gaatgaactt cattctgtct cagatttttt gttatttcaa
     7861 attgattaat tgattgatca aaaaggtttt gattttcatg ttctcaaaca cgttcaatgt
     7921 ctttttcttc tgaatggtct tgtaagagat ctctgtaatc atcaaatttt tgtctattaa
     7981 tcttagtatt ttttgatgta taaactacct gaaattgaga gtacatacca aattcataaa
     8041 ttttttatta gtgggactag tgattgtatg gattttgacg cataatgaat gaagtatgag
     8101 aaaaggagag acaaatggat caagaactat ttattatcga agtattatca gctaaacaaa
     8161 aaattatcga gaaagcgaaa aaaagagcga aaagaataat gttcaagctt ttaagcttag
     8221 tatttttctc tggtttgctt tttttcggag ctgtatcgtt cctatatcgc catatgagtg
     8281 cagtagtccg ttcagctgaa gggatgcaaa aattaaataa tttgtatcac ttttcgtggg
     8341 gagttggcgg aataacaatt gttgtgctta ttgtttttct tctacttatt tggctccgtt
     8401 accatcaatt gtcgcgtcat ttcgatcaat ggcttagtca aagagcacaa cagtttttac
     8461 ataataaacc aacttctcaa gatgatgact atctttattt tgagaagact tcgacgagtc
     8521 aagcttttcg gatacaaaaa agtgattgcc agttattgct gagatcactt gatgatttac
     8581 ctatctactt aggcgaaagc aaatcaaaat tcggaatgaa cacattccat atttttttac
     8641 tagaaaacga acccagatgg gaaaaaagcc agtcttcgcc tggtggatac ctgacaacta
     8701 agatgagtac attagtcact ggttttttcc tcattctttt agctggaaca atctattatg
     8761 ggagatacac tcagccaaaa atgtcttctc atataaatgc tgcttctcac atgacaatga
     8821 actcaactaa cgatacgcat atacaaaaga caaatgaagg gttgttagct gatcctaagc
     8881 aaaaggtaaa tttgacaaca caaaagataa atgatctcaa acttgataca gagacgcact
     8941 ctctctatat gacaacagat agtggtgcta attgggtatt tgtgccgatt gattcgcaat
     9001 ggttgcgttt tggcgaatac accctgacaa cggggctgat tcctgaaggt tattggatgg
     9061 acaacacata tgatatttcc gccgatttct catggtttat ctattcttct ggggatgatg
     9121 tacgtctgct gacttcaaga gataatggta aaacatggca aacgtctatt atcacccaat
     9181 cagttcagcc gatacgttat cgaaaagcgg tattttatgg caacgatggt ctatttatct
     9241 gtacatcagc cagtgggatg tctgcagaaa ctttgtacat ttacactact atggatggcg
     9301 gaatcacttg gataaaaagt ggaagtacaa tgatcaatca gccagtacaa aatgtcagct
     9361 tcgttacaac ttctttaggt tttgtttcga cacgtgaaag tttgttttat acaaacaacg
     9421 gaggacgctc attcaaagaa tcagttctgt cgattccgga aaactatcag acagggggga
     9481 tagatctttt tcaatcacca aatgaagtta ctcaaataag cgcgaatacg ttagagacaa
     9541 aattttattt gttgaaaaaa ggaccaatcg atgcagggaa aatgtttgcc tgcctcttcc
     9601 gttcaactga taacggggaa acatggcaat tcgttcagca actatcgcaa gtagaacaag
     9661 gagattaaag aagagagatt gtgagcctga cgttcagcac cgagtcacaa gaaacaaatt
     9721 caaaaaacag cttctcatat ttgttgaata gtgtaactcg tgattcggag ttgacaagag
     9781 caacaagaag cgattttgaa gtatagcttc tcgtatttta caagatcgtg tcttattgcc
     9841 gataggttgt ctcttgaaca ccgtttattc gtaaaaaggg attgtgacaa gtcttttgtc
     9901 actacttacc gaacgaaact gaaggagcta tccaaaaagt atcattagta ttattctttg
     9961 tgtttctagt ggcaccacta gtacatttaa aaaagctatc gatcatggac taatcataca
    10021 tgatcgatag ctttttagat aatgtttgtt acgctatctg aaattgacta ttataaagac
    10081 tggcataaag cgttggtgat tggagtagtt cagaatgtgt tcctttttcg acgatatcgc
    10141 cattcttcat tacaaggatc atgtctgcat tttcaatcgt tgacaaacgg tgtgcaatca
    10201 caaaacttgt cctatctttt gtcacagctt ccattgcttg ctgaatcaat gcttctgttc
    10261 gtgtatctac gctagaagtt gcttcatcta aaataacgac aggaggattc gctagaatga
    10321 ctcgagcaat tgtcaataat tgttgctgtc cttgagataa cgtgccgttt tcgctagaaa
    10381 tgatcgtatc atatccgtct ggcagtgttc gaataaagtg atcacattgt gcgatttcag
    10441 ccgcttcgat aatttgttct cttgtggcat ccattttccc gtaggcgata ttatcagcta
    10501 ccgttccttc aaacagccag gtattttgta aaaccatccc aaaagaacga cgtagttctc
    10561 cacgtgaaag cttggtaata tcaagaccgt cgaattcgat ctttccttta ttcagttcat
    10621 aaaaacgcat cagcagattg actaaagtcg tttttcctgc accagttgga ccaacgatcg
    10681 ctaccgtttg ttttggcttt actgaaaaat cgacttgatt catcaatatt ttatctgatg
    10741 tgtaaccaaa ctgtacattt ttgaaagcta tttttccttt cggttgttct aaaagtcttg
    10801 catctggaag gtctggaatt tcatctgttt catctaatat ctcaaatatc ctatcgattg
    10861 ctgccattgc ggattggatc gagttgatca cataagaagc ggtagaaatt ggttcagaaa
    10921 tctgattgat gtactgaaga taagcttgta agaagccgat cgtcacacca cctgatagaa
    10981 caagcatagc accgaatacc gcacttatga tgaaggcgag ttgattaatg aaacgaatgg
    11041 ccggatagat agcacagttc ataaattgag cacgtttaaa agcgttgtat tgctttttat
    11101 ttacttcgtg tatagcagct tctgcatgag cttgctgatt gaatgttttg accactagat
    11161 taccagctaa gatttcttcc attcgattat ttaaaacacc cagttcatac tgattctgtt
    11221 cagaaatctg tttgtttttg ttcgcaatct tagttgtaac aattgaactg ccaacgatca
    11281 gcaataagac gagcaaagta agcttgcttt cgatataaaa cagcatcaga ccagcagcag
    11341 ctattgttac gacagaagta aaaaattgat tgatacctgt caataacact tgagaaagtt
    11401 gattgagacc ggtagttgtc cgactaatga tgtcacctac ttgatgattg tcaaaaaacg
    11461 acatcggtaa tgttttgaat ttttttgtga tttcttttcg caccctcaat gtgatctttt
    11521 cgcttaacgc tgccattgtc cgttcctgaa taaatgaagt gatgctgctt aagactgaac
    11581 agccgatcag caataagaca gggaacaaca atgcttgttt gatatcactg aaagaaagct
    11641 gtccaatacc tgtttttctt atttctgata agagatcatc gatcccgatt cccatgatca
    11701 agggcatggc tacgactaat aggttcccga ataaactgca tagaaacaat ccgtaaaaat
    11761 atttatgttc aggtttgatc attttccaaa aacgtatcaa agcacgcgtg ttcaacgttt
    11821 ttttcttttc catcgtatcc cttctttcta tattccttgt gattttgcaa actcctgata
    11881 agtttggcaa ttagacagta gttcttcatg agtaccttgt ccaacgattt ttccttcatc
    11941 taaaacaatg atgttatctg cttgttggat tgtactgagg cgttgagcaa caatcacgag
    12001 agttttctcc gacatgtgtt tatgaagggc tgctcttaat gctgcatctg ttttatagtc
    12061 taaagcggag aaactatcat cgaatacata gatctctgct ggtttgatca acgctcgagc
    12121 aatacatatc cgctgtttct ggccccctga ataattagaa ccgccttgag caacaaagct
    12181 gtccaatccg tcaggaaggg tgtccactag ttcttctaat tgagcgatct tgatcgcttt
    12241 agacaactct tctgttgagg catattcatt tcccatctgc aagttgctta aaattgtccc
    12301 gctgaataag aaagccttct gagggacata actgatgtgg gagcggatct gttcttgagt
    12361 catttgagta agaggaatac caccaagggt tacttttcct atcgtagggt cactaagtct
    12421 caagagaagt ttcgccaaac tactttttcc agatccggtc ccgccaacga tcgctgttgt
    12481 ttttccttta ggtattgtga aatcaatatc ttctagaact ggatcagaga tttcagtata
    12541 tttaaatgtg acatgttcaa aagcagctgc atttgtctga tcaaaggttg gttcagctac
    12601 tgttttgatc gtctcttcct caatttcgat ctctgcttgc agtatttctt ctatccgtcg
    12661 taatgaggca aacgatttag ggataatgac caataccata gctgcgatca tcaagtaagc
    12721 aagtgtcagc atagagtatt cgactacagc agtcacgaca ccgatttgta atgctccttc
    12781 tgctacaaga tttccgccaa accacaaaat agaagcataa acgattccca tgagcaaaaa
    12841 agcaattgga gtgacccaag caaatattcg gttcactttg atcatttgat tagcatagtc
    12901 agcaaagctc ttattcgttc gcttttcttc atatgattga ttatcaaagg cacggatcat
    12961 attaatccca gtaaagaatt ctctcaatgt gatggtgatc cgatccattt ttggctggat
    13021 actcaaagat aaaggtgccc ctcgtttcat cagtcgataa acaatccata cataaaaaac
    13081 aacagagatc aatgtaaccc atgttagtaa tagagaataa cgaaacatca tgagaatggt
    13141 gaaaacgcaa ataaccggtg ctggtaagat catttggcaa aataaaacaa tcatttgctg
    13201 gacattatcg acatcattcg tcattcttgt caaaagagat ccagaactaa aacgactgat
    13261 atccttcatt gacagctgtt ggaatttacg gaaatatttc tcccgcgttt ggaatccaaa
    13321 cctagcggaa gccaatgctg aaaagtaact agaggcaact gcagcgattg ttcctagaaa
    13381 cgctacaagc agcatttcaa ctccaatttt ttttataact gaggcttcgc cacttgctat
    13441 acctgtgtcg atcagctttg ctactaattt tggtacacct aatgtaccgg cgatttctaa
    13501 acagatcatc acaaaggtaa ggagagcgag tcctttgttt ttctgtaaga atgtttttaa
    13561 gatagacata ttattcctcc ttttttcctg atacttttcg attctatacc ttcaagtaac
    13621 ttgaaggtca atagaaaaaa gaataaactg aaaaagaggt gagaaaaaat gaaaaaaatg
    13681 tatacgattg gccaagtatc ggaactattt gatttgccaa aatcaacgat ccgctattgg
    13741 gatgaacaag gattaatccg ttcaagtaga caagaagaga atgattatcg attgtttgat
    13801 atcgatgata tttttatgct ttatgatatc gttttttata gaaaattaga tataccgatc
    13861 aaacagatga agaatttata tggaaaaaca ttgacggagt tatatgagac acttgatgaa
    13921 acagaaagaa gaatccataa agaactggtg gtgatgaaac aaaaacaaaa agagataagg
    13981 gagcaaaaaa aacagttgaa attgatgatc gatacaaacg aggaagaatt tcctgtagaa
    14041 gagatccctt ttgactgtat gatctcaacg gagtttgaag atattgtgga aatcaaaaaa
    14101 tttctaccta actattcaag ctttgggatg atgagtatgc cagcttccag ttcacagacg
    14161 atgtatggat tttttatcga ccctagtgaa gtacatttat ttactcaaga cgtgatttgg
    14221 gagaaaaaag atacggctgt ttatcgtcga ttcttattga aatccgagat gaaccatgcg
    14281 gaacgtaata atattttaga aatcagagaa agaatgtatg aaaaaggatg gaaaacagga
    14341 gaagtgatcg gccagtatct attgacgaat acggatgaaa acaatatcag aactgaatat
    14401 tatcatgcat ggatagaaat gaaaaaatag gaaatatgtg tttaagataa ctcgaaaatg
    14461 atggttgttt tagttataaa gaaaaatttt ctggttcgat tacgggttta atgtggggga
    14521 ttattagtcg tggatagata gaattttagt tttttagagg aaatatgtct ttaaaaatgg
    14581 aatggttgca attcttagcg atatatacga tgtaattgtc ctcttaaaaa agtatggaaa
    14641 aataattgta ttgggtgtta tgtataattc atggtgaact gagactaatg agaagaaaaa
    14701 ttcatattgt tatgagtgag attgtatcag atgagaatga taaattaaat aggtgcataa
    14761 aagcattaca cttaaaatac attgttgctt acattattac aatatgttaa taaggcaaaa
    14821 ataaatagat ggattgaata aacatatttt ttaatcaaat agtccctact gtgactttcc
    14881 tcagctatcg gtgatactac cgatagcatt tttttgctta taaaataatt ataagtattt
    14941 agagattttt cttctaacat tcttcaaaca agaaaaatca caaaggttat tgttattttt
    15001 atatattctt tttaagaatg attgtttttt tatagaaatt ttaaaaaata aaacacgtta
    15061 ttgatggata gttagtttgt tatttggtaa tattatagaa gttgactggt agtgatttat
    15121 ttttcgtcgt tttataaaaa tgttattttt tggaaaaatt cacaaatatg taacgaatgt
    15181 ttttctggaa tttgctgtgt taaaaagcag tatgactaat ataaatacta ttgtcattta
    15241 tgcaattttt tatggatagt aattttagag gggaatcaat gaaggaagaa tagaagggaa
    15301 aaatagttgc aaatacgcca gttagcaaaa atagcaatac atcttcaagc tttaaaattc
    15361 gagagaatca tcttggtagt ggaatcaact acaacaaaag gattaaacca attaatagat
    15421 agaattacca atggaaaaat agaaaaggta gttattctat acaaaagttg tttaattcgc
    15481 ttcggttatg aacttagtga aaccctttgc aatatatggt acagaatata gaaattattg
    15541 acattacaga aaatgatgaa gatttaatac agattgtcac tatttgtaga taccttttac
    15601 aaggaaaccg agccaataaa gctaagaaga tgattaagga gttgatggaa gacgatacaa
    15661 gttaagaaag tgaaactacg cccaaataaa gagcaaaaag agtaattgga cgtggaaaat
    15721 gcaagaaatg aaccatcgat tcggtggaca gtttattagt aatgacggtt tgagaacatc
    15781 catgacgaga atgaaggaac atcctaaatg cgtttagttg aaccaagtgc caaataatat
    15841 ggccaaataa gcgatcagtt atcgatgaat cccaagtatt ctaaactata tattcatttt
    15901 gatgctaagc attgatatat ttttttggga ttgatgaagg ttaaccaata cagggactca
    15961 ctgatgaaat tctagagtct gaccttggta tcaacgattt agtgattgtt tctaatggaa
    16021 aagtataaaa gtatataagt acattaacaa aataactatt agggttacgc tccagcttgt
    16081 gcaaggcact ggcaggagga atcgtgaaat cataattatg gttaagccac acggacaact
    16141 aaaagtcata caaagctatc gaatcaatca acagaagaca atcggcaatc attagctttg
    16201 tttacagctt acattgcaac gcttgagttg aaccgaaaat acgagggagg taacgcagcc
    16261 attttgagtt ggggaatctt tttagaaaat tatcgggtac cttgttgttt agtcaaaatt
    16321 ataaggggga gtaatagtga ggaacaaatt tcataaactg ttaagtatgt ttctagttat
    16381 gcttattgtg atcaatcaat ttgggaacat aacggttcta gctgagtctt tagcagaaca
    16441 gccagagcgt tcgctgacga tcgaagttga tcaagaggag gctgctttgg gtgagcagtt
    16501 ccagttgcga gtcagaggaa caccagacct gctaaatgca ttggtcattg ataagagtga
    16561 ggatttgatt gaaggggaag tgaagcaact caatgattac gaacgtctga ttacttattc
    16621 ttcccagcat tcaggtaaat acgaattgac cgcaagaagc ggtgatattc aatcaaatac
    16681 ggtctcaata gaaatcagtg taccagaaga aagcacaagt gagtctttta atgtaccaga
    16741 agaaagcaca agtgaatctt ttaatatacc agaagaaagc ttaagtgaat cttttaatga
    16801 agccagatca tctgagccat ctataacgca ggaatctcag attgaaagac aagaggagct
    16861 cttgaattat gacatcggca tctctgctga gcgccccact ttgccgagca ggcatacaca
    16921 aacgttaaag ggatacattc aaggaggcgc tggcgatatt cttgaagaat atggggagat
    16981 ggtcgtacga gttcctagag catcgattgg cttaattgag ttcattagag tggaggtcgc
    17041 tgatgattca cctttaaagc cactggcatt ttaccaagaa ggcacagacg ttgtttttcc
    17101 atttaccttt gattatgaag gattaaatga agccgctcag tcgtatacat tcagtatcac
    17161 gtttgaagtt gctttctttc aatcaggcgg tactgagcca aataatgact ttcaagtgag
    17221 tcttacttat gctgagcgag cagcaaacac ggaattttca attacgccta ttccttcatc
    17281 aagacccatt ttttcgaaat tatactatgg ctctagagaa atcgacagcc aaggaaatct
    17341 tgggaatctg ctgattgacg ggcaagacgt gaccaacagt aataaattta ttttgcaggt
    17401 aaattatgaa ggatcacgaa aagacggtgt agtggttacc gatgtactac cgcaaggaac
    17461 aactttggca ccgaatccga ctacatggag ttccactgga aatagagaac ctatttcagg
    17521 tggggttcgg atcgcagaag taacgaatcc aggtgaagca aacgttagaa ctcattatgt
    17581 ttccgaggaa tttgtggaca aagtatcttt taataaggaa acgaatacac ttgttgtgga
    17641 ctttggtaat ctagcagagg gtcaatcgta ttttattgaa tatgctcttg aagtgaccga
    17701 tgtcgacttg ttcaatgaag gaagacaaga ggatgggttt tctcggaatc gagcgagtat
    17761 gacctacaat aatcaggtaa tgagccgaga ctaccgcttg agaatccctg aaagtaatct
    17821 tccggcaatc tctttaagaa aaagtgtgga caaaaatgtt atcaatctca atgaaaatga
    17881 actaacgtat actctgcatg tctcgatgtt ttctggaaca agaaatacgc ttgttattga
    17941 agatgttttg gaagaaggca tggaatttgt gtcatttcta tcagatattc cctctttcat
    18001 tgatgccgaa gtcaataatg gaatagttcg tttcagtgcg aataatacga ttggtggtcc
    18061 gacaagtttt accctctctt ttaaggtcaa tgtagaggcg tatgcggtcg gcgatcaaat
    18121 tttaaacttt gcaacgcttg tgaatagtga caatcgattg aatacgagta ctgtaagaac
    18181 aaggaaaatc gatgggcgta ttaaagtaat taaaactgat gagaatggcc aaaggctggc
    18241 aggagcaaaa tttgaaattt tatcaagcga ttcacaagtg atacaagaag gtataacggc
    18301 agaaaacgga gaatttttaa gtgaaccgct tcctgtaggt agctatcaag tccgagaaac
    18361 agaagcaccg gaagggtatc ttttagatgc gacggtacat gatgttttta taaatgaata
    18421 ctccatagaa gaagtgacgt taaccattga aaacatcctt gatacgggtg gtgttgaact
    18481 aattaaacat gctgaaggca gtggtgaagt cct
//
LOCUS       DACCKU010000057        18504 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.060, whole genome shotgun sequence.
ACCESSION   DACCKU010000057 DACCKU010000000
VERSION     DACCKU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 18504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.060"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IVN13_001849"
     CDS             99..746
                     /locus_tag="IVN13_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP7012321.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IVN13_001850"
     CDS             1139..3784
                     /locus_tag="IVN13_001850"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP7012322.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IVN13_001851"
     CDS             4094..5407
                     /locus_tag="IVN13_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP7012323.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IVN13_001852"
     CDS             5397..6050
                     /locus_tag="IVN13_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7012324.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6797
                     /locus_tag="IVN13_001853"
     CDS             6105..6797
                     /locus_tag="IVN13_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7012325.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6947..7147
                     /locus_tag="IVN13_001854"
     CDS             6947..7147
                     /locus_tag="IVN13_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7012326.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(7401..9080)
                     /locus_tag="IVN13_001855"
     CDS             complement(7401..9080)
                     /locus_tag="IVN13_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP7012327.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(9082..9294)
                     /locus_tag="IVN13_001856"
     CDS             complement(9082..9294)
                     /locus_tag="IVN13_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP7012328.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9687..11417
                     /gene="efrA"
                     /locus_tag="IVN13_001857"
     CDS             9687..11417
                     /gene="efrA"
                     /locus_tag="IVN13_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP7012329.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            11414..13174
                     /gene="efrB"
                     /locus_tag="IVN13_001858"
     CDS             11414..13174
                     /gene="efrB"
                     /locus_tag="IVN13_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP7012330.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            13269..13466
                     /locus_tag="IVN13_001859"
     CDS             13269..13466
                     /locus_tag="IVN13_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012331.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            13619..14104
                     /locus_tag="IVN13_001860"
     CDS             13619..14104
                     /locus_tag="IVN13_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP7012332.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IVN13_001861"
     CDS             complement(14208..14801)
                     /gene="clpP"
                     /locus_tag="IVN13_001861"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP7012333.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            14981..15508
                     /locus_tag="IVN13_001862"
     CDS             14981..15508
                     /locus_tag="IVN13_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP7012334.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            15599..16336
                     /locus_tag="IVN13_001863"
     CDS             15599..16336
                     /locus_tag="IVN13_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP7012335.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            16340..17257
                     /gene="miaA"
                     /locus_tag="IVN13_001864"
     CDS             16340..17257
                     /gene="miaA"
                     /locus_tag="IVN13_001864"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP7012336.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            17259..18488
                     /gene="hflX"
                     /locus_tag="IVN13_001865"
     CDS             17259..18488
                     /gene="hflX"
                     /locus_tag="IVN13_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP7012337.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         5983 a         3201 c         4023 g         5297 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt atcttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttattccat
     6661 cgcagcagga tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca
     6721 ctgtactgga ccacttgatc atcggacgca aacgatattt tagtttgaga gaagaaggaa
     6781 tgatggaaga gaagtaagtt gctttcttgc aaaaaaaatg aagcaagtgt acaattatat
     6841 tgtaattttg tttgggaata gaagttgaaa catctgttgc acctctatcg tacacgagca
     6901 atgttacaat ttaaattagt gcttttggca cgaggaggat attattatgg aaaacggaac
     6961 agtaaaatgg ttcaacgctg aaaaaggatt tggatttatc tcacgtgaag atggaagcga
     7021 cgtattcgtt cacttctcag ctatccaagg tgacggattc aaaacattag aagaaggtca
     7081 agcagtgact ttcgacgttg aagaatcaga ccgcggccca caagctgcaa acgttgtaaa
     7141 agcttaattt tatctgtaaa tcaaattgat ggtagacatc aatttttacg agagaacaaa
     7201 gcgagtaaca aaaatacctg ccacaagaga caatttctct tgtggcaggt atttttttgt
     7261 atgaaaaagt aaataaattt aagaggctat gaaagagcca ccttgtatca aggaataaga
     7321 acaaacaaaa atggcttctc atgtttttca tttgttttct cttatattca ggacaaggtg
     7381 ctgatttcat aacctcttat ttattcttca tctggtgtca ttaccattgg caaaatcatt
     7441 ggatgtcttt ctgtacgttc gaataagaaa ggctgaagcg cgccagtcat cgcttctttc
     7501 aatttatatt ctgaacagtc aggtccattc aaacattctc ttaaagcgtg gaataagatt
     7561 cgctgagctt cattgatcat ttctcctgat tctcgcatgt agacaaatcc acgagagaga
     7621 atatctggac cagccaagat ttcttttttc ttgatatcca ctgtcgcgac agctaataca
     7681 agaccttctt ctgaaagcag gtgacggtca cgtaaaacga cattaccaat atcaccgatc
     7741 ccgctaccat cgatatacac atctccagcc acaaaatgcc cagcaggacg cgcagaatcg
     7801 ctagttaacg ctaaaacatc gccattttcc atgatgaagc agttttctgc aggaacacct
     7861 acatcttgtg ctagagaagc atgtactttc aacatgcgga attctccgtg gacaggaata
     7921 aaatattttg gcttcatcaa acgaagcatt aatttttgtt cttcttgtcc accgtgaccg
     7981 gaagtatgga tgttattgat ttttccgtga atcacttctg caccagcttc agacagcaaa
     8041 ttgatcaaat gattgacact tgttgtatta ccaggaatag gtgaacttga aaagatcact
     8101 gtatcgtctg gctggatttg gatttgacgg tgagtaccat tcgcgatgcg gctcaacgcg
     8161 gccatcgctt ctccttgaga acctgtacat aggatcatcg tttcattggc aggcaagcga
     8221 ttgatttcgt gtgcatctac aaatgtgccg tctggaactt tgatatatcc taaacgttga
     8281 ccgttgacga tggcattttc catgcttcgt ccgaaaacag cgattttacg accagttgcc
     8341 acagcggcat ctgctgcttg ctgcaaacgg aagatgttag aagcaaagct ggcaaaaatg
     8401 atacgtcctt cgatttttga aaagatcttc aaaatagaat gcccaatcgt tttttctgat
     8461 ttagtaaatg ttggaatctc agcatttgta ctatcagata aaaggcacaa gactccttct
     8521 tccccgatac gagccatttt atgcaagttt gctggttcgc caacaggagt gaaatcgaat
     8581 ttgaagtctc cagtcgctac gatatttcca gaaggtgttt tcaccacgat ccctaatgca
     8641 tctgggatac tatgggtcgt tctgaaaaag ctgacagaag tctttctaaa acggatgatc
     8701 gtatcttcat tgatctcatg gagctctgca tctcgaagca agccatgctc atctaatttg
     8761 tttgtgatca acgctaatgc gagaggacca gcatagatag gaatatttgc ttgtttcagc
     8821 aagtagggta ttccgccgat gtggtcttcg tgcccgtgag taatcaccaa ggcttttact
     8881 ttgtgtaagt tttgtatgat gtagctgtag tcgggaatga catagtcaat tccaagtaaa
     8941 tcgtcttccg ggaatttgat cccggcatca atcaaaatga tttcatcttg gaattgtacc
     9001 ccatacgtat ttttcccgat ttcacctaat ccaccaatag caaagacgcc tgtctcgtta
     9061 tttttgatgg aaagtttcat attaaaactc cactaatgag aaatcagcat tttctttttc
     9121 gtactctaga tgacttcctt ctagaagttg aacgaattca atattgtatg gggtattttc
     9181 ttccacacgt tggcgagcgt ctacatcgct ttttgcctca atgtaaagag attttgtttc
     9241 ttcccgttta gggttgcgta cttttgtttc ttgataataa actttataga tcattgtttt
     9301 gtctccttta tctgcgtaaa gcagtgattt agctagttga tttttttaga caacacaaaa
     9361 cgaggggaca agccacctgg tttttcatat gctttgacta gaatactgga ttcgagtcaa
     9421 agagacaagc ctcgtttttg cttatctgtt cggatatttt ttcaaccgta ccttctgtat
     9481 aaaaaatata caacaattaa ttgttattct agcataaaac agcaaataag tatagaaatc
     9541 aactagattg aaagaaaaaa tcaatgttta tcatcccgct ataatcataa tgactttttt
     9601 atgagagaag tgttctatta ataggctcgt tgtgctatca tttttagtaa tgaaaatgta
     9661 acgaaagatt aaaaggagat gagaaagtga aactaatgtg gcattatacc atgcgttata
     9721 agaaattctt gtttttcaat ttcatatgcg tatttggttt tattttgatt gaattaggac
     9781 ttcctacgat tttagcacgt atgatcgatg taggaatccg caatgatgat tttgattatg
     9841 tgaagcaaca agggatattt atgatcgtta ttactgtaat cggtattatt ttgaatatct
     9901 tgttaggtta ttttggttcg aggattacga ctaatatcgt agcagatatt cgagatgatt
     9961 tgttcgaaaa gatccaaagt tattcgcatc aggaatacga aaatctaggc gtatcttcat
    10021 tgatcactcg tacaacaaac gatgcttatc agatcatgtt gttcctgcaa aatattttac
    10081 gtatcggttt tatggcacca atgatgttca ttgtcagttt gtacatggtc atgcgtacaa
    10141 gtgtgacact cagtttgtat gtagtaggtg cattaccttt actattattg gcagtcgtag
    10201 cgattgctaa ggtgtctgaa ccgttatcta aaaagcaaca aaaaaactta gataaaatca
    10261 acagtatctt gcgggaaaat ctttcaggat tgcgtgtgat tcgtgcattc gtcaatgaaa
    10321 agtttgaaga aaaacgtttt gctgtagtca atgagaatta tgcttctagt tcaaaaagct
    10381 tattccgttt gatggctgtt gcacaaccgg gtttttattt cttatttaat atcgtaatgg
    10441 tattgatcat ctggagcggg gcagtgcaaa tcgatgcagg cgagcttcgt gtaggagatt
    10501 tgattgcgtt tatcgagtat atattccatg ccttgttctc attcatgtta tttgctagtg
    10561 tgttcatgat gtatccaaga gcagctgttt cagctggacg tatccaagaa gccctagatg
    10621 ccaaaccttc gattgtagaa gatccaaatg gtatatctga tacaaaaact aaagggtata
    10681 tcgaatttca aaatgtaaca tttgcttatc caggccattc gcaagagcca gtgatacgaa
    10741 atgtcagttt tacagcctct ccaggagaga ccgttgcttt tattggaagt accggtagcg
    10801 gaaaatctac gttgatccaa ttgatccctc gcttttatga tgtgagtgaa ggaaagatct
    10861 tgatcgatgg agtagacgtg agagattatc ggctaagcaa gttgcgagat aagattgggt
    10921 ttattccgca aaaagcgctg ctatttactg gaacgattgc agataatttg cgatacggga
    10981 aagaagatgc tacgcaagaa gaaatggaac gtgcagctga gattgctcaa gcttcagaat
    11041 tcatttctcg aaaaccagat ggatttaatg aacatttatc agaaggtggt tccaattttt
    11101 caggtggaca aaaacaacga ttggcaatag ccagagccat catacgccgt ccggaaatct
    11161 atatttttga tgacagcttt tctgcattag attatcaaac ggatgcgaag ttacgtgctc
    11221 gtctgaaaaa agaaacgaca gaatctactg tattgatcgt agcacaacgt gtgggaacga
    11281 tcatgcatgc agataagatc atcgtattaa atgaaggaga agtcgtcggt atggggacgc
    11341 atcgggagtt gttggaaaaa tgcccgattt attatgatat tgccgcttct caattatcaa
    11401 aggaggaatt agcatgaaaa aaaccttttc ttccttaaaa cgtttagctc gatatatcag
    11461 accgtatcgt gagacttttc ttttagtgat cctatttaca gttctgacag tggctttcaa
    11521 cgtggctttg ccatatgtta caggacttcc tactacagaa atcagccgta atattgccaa
    11581 tggcgaatca atcaattttt cttatatatt caaatgtctc atttggatca cagttgttgg
    11641 aatagggtat tgtatttcgc aattactttc tggtgttttg atgacaaatg tcgttcaaag
    11701 tgccatgcat gatttgagaa gagacataga agaaaaaatc aatcgtcttc cggtttcata
    11761 tttcgataaa aaccagcaag gaaatatttt gtctcgtgtg acgaatgatg tagatgcagt
    11821 cagcggtgct ttacaacaag cttttattgg tatcgtaaat gcgatattgg gaattacgtt
    11881 ggctgcagca atgatgtttt atatccagcc gatgatggcg ctgatctcta tgatcatgat
    11941 tccaacatcg atctggatct caaaacgagt gatcaatgct tcgcagaaat attttcaaag
    12001 catgcagaat tctcttggtg agctaaacgg ctacgtccaa gaaaatatga caggttttag
    12061 tgtattgaaa gtatatggac gcgaaaaaga aacattggaa ggattcaaaa aagttaatca
    12121 cagcttgaaa tactttgggt ttcgtgcagc attcatttct ggcttgatga tgcctctagt
    12181 tcaattaact gcctatgcta cttatattgg tatggctgtt ctaggaagct tttatgttat
    12241 tactggggcg atcgttgtcg gtcaactaca agcattcatc caatatattt ggcaaatcag
    12301 tcagccaatg gggaacgtga cacaattgtc ttcagttttg caaagtgcat cagcggcaac
    12361 gaaacgtgta ttcgagattt tagatgagcc agaagaaaaa gaaaacaaag tagatgcttc
    12421 actgccagaa acgattttag gagatgtgac attcgatcat gtcgattttg catatgatcc
    12481 gaagaaacca ttaatccaag atttgaattt tgaagtgaaa gctggtcaaa ccgtagctgt
    12541 ggtcggtccg accggtgccg gcaagacgac attgatcaat ctattgatgc gtttttatga
    12601 cgtagacaaa ggagcaataa ggattgatgg aatcgacacc aaatcaatga gtcgcagtga
    12661 tgtgcgttcg ctatttggta tggtcttgca agatgcatgg ctttatcaag gaacgattgc
    12721 agataatatc cgcttcggaa agcttgatgc gacagattat gaagtagttg atgctgcgaa
    12781 aacagcaaac gtagatcatt ttatccgaac gatgccagat ggatatgata tggccatcaa
    12841 ttctgaggga gacaatgtgt ctttaggaca aaaacagttg ctgacgattg cccgagcagt
    12901 ggtctccgat cctaaaatct tgattttaga tgaagcaaca agttcagtag acacccgttt
    12961 agaagcgttg attcaaaaag caatggatcg cgtgatggag ggacggacaa gtttcgtcat
    13021 cgctcaccgt ttatcgacaa tccgtgatgc agatctgatt ctagtgatgg atcaaggtca
    13081 gatcattgaa aaaggaacgc atgagagttt attggcacaa ggtggatttt atgaaaaact
    13141 gtataacagc caatttgctg aggaagcaga ataagtcata taagaataaa acagtttaaa
    13201 acttattttt tgttctctat tcttgataat ggtaaagtaa agttatcaag aaaagtgagg
    13261 acacggagat ggaacagttt ttaaatgaaa aaattactga agccaaaaga gaagttgatc
    13321 gtttaacgca cgtctgtcag acgaagcctt ttgattcagc tgattttttt gaaaatgagt
    13381 ttcaaatcga acgcttggca gcacaaatag aagtgtacga attaatttta aaaaaagtaa
    13441 tagaagaaaa tatagatgta caataaaaac aggtaaaacg tcagttacca gatactgtaa
    13501 atagacatct ggcctgagca ggttttacct gttttttttc ttatgaaata aatatatgct
    13561 atgatacctt tattcaatag gattagtttc tacaaaaaat gaaaggagaa aaataaatat
    13621 ggaacggttt atagcatcaa atgccacagt gattggtgat gtgacattgt cagaagatgt
    13681 gacgatctgg tatcaagctg ttttacgcgg ggacagtaat tggataaaaa taggacaaag
    13741 aacgaatatc caagatggta caatcatcca tgtcgatcat gatgcacctg tagatattgc
    13801 ggagaatgtg acggtaggac atcagtgcat gctacatggc tgtacaatcg aaaaaggggc
    13861 gctgatcggg atggggacca cgatattgaa tcatgcggtt attggtgaga atagtttgat
    13921 tggtgctgga tcattagtaa cagaaggaaa agttattcct cctaatgtct tggcttttgg
    13981 tcgcccagct aaagtgatcc gtcctttgac aaaagaagaa atacaaaaaa acaaagaaaa
    14041 catccagcat tatgtcgaga taggtcggat gcatgcgcaa ggaaaattca aggagcaaag
    14101 ataggcagta gcatccataa aataaaaaag gatggtcagt ttgagttgac catccttttt
    14161 tatttggttt agataagaga gcttaaaaaa gcttcctttg aaacagatta atttaatgaa
    14221 gaactgtttt ccatgacttc atcgatcaat ccatatgctt ttgcttgttc agctgtcata
    14281 tagttgtcac gatctgtatc tttttcaatc acttcaagcg gttgtccagt gcgttcagct
    14341 aaaattttgt ttaagcgttc gcgtgtttgc aaaatatggc gtgccgcaat ttcgatttca
    14401 gttgcttgcc cttgagcgcc gccaagaggt tgatggatca tgatctcagc atttggtaaa
    14461 gcaaatcgtt taccttttgt accagctgtc agcaagaagc ttcccattga agcagccata
    14521 cccataacaa ttgtttggac atctgctttg acaaagttca ttgtgtcgta aatagccata
    14581 cctgctgtaa ctgaaccacc aggagaattg atataaagat agatatcttt ttcagaatct
    14641 tgagcgtcta agaaaagcaa ttgggcgatg atcgaattag caagatcatc tgttacttgg
    14701 ccgcttaaca tgatgatacg gtctttcaat aaacgtgagt agatatcgta agctctttca
    14761 cctcgtgaag attgttcaat gactgtagga attaaattca taatagagtt cctccttgat
    14821 ttcgtttaga aatagtgtac cataatttag tttctaatac ctaaaatgta gtatacattt
    14881 ttggtcaaaa aaggtcaaat aaaaaactta ggcaaattag tcgcataaac ccgctttatg
    14941 gtatgataag cagagaaaca aaacaaggag gaacaacaga ttgatagttg gtattactgg
    15001 ccttatcgga gtgctgacag gtgctgtagt tgtctatatc ggcatgatac tgcaaatgcg
    15061 tcacttggaa aataaagaac agcgtagacg tgaattggaa ttgaaagtca aagaaatcga
    15121 gacgctgaat caattaaata aaaagatcaa cgagatcttg caaaaacgga caacgctgat
    15181 ggaagaatat gtcagctttg atgcatttga tgattgttat atcacgattg acgattttgc
    15241 gtatctccaa tcattttcag cgcaaaatag tttttatctg ccaaattata ttttagacga
    15301 gctatttaag aatatctccc atcgaaaagt gattttatct ccagatgaaa caatgaagat
    15361 tggtggatat gcttataaag gcggacgcgt gattttagag aattttacgg atagtctgac
    15421 tgaaatgatc aatgaaaaaa aagctcagtt gaagactatg acgcgccagc cgattggttt
    15481 cttctcgaaa gatgatgttt ggagctgata ttttgaagca ttgccgttgc acatgagagc
    15541 gtggtaatgc tttctttttt tcttaatgat acaatagaac tgaacggagt gaagaaagat
    15601 gttacaaatc tatgcccatc gcggaagcag cggcacccac ccagaaaata cgcttcctgc
    15661 atttgcagaa gctgtacgag taggtgcaga tggtatcgaa ctagatgtcc atctaagcaa
    15721 agatggttat ttgatcgtta tgcacgacga agaagtggac cggacgacta acggtaaagg
    15781 gctgattcga gaaaaaacat tggaagaact caaaaaattg aatgcagggg gcaaattcag
    15841 caaagaattt ccagcagcta aagttcctga attgagagaa gtgttaggac tgctcctcca
    15901 aaaaaattac cgagggatgc taatgattga aattaaaact gatcagtatg aatatcccgg
    15961 tatcgaagaa aaattagccg aaacattaaa aagtagagat tggccttttc aacatgcata
    16021 ttgcagcttt catttggatt ctttggaaaa aatcgcatta ctggaaccgg agatacagct
    16081 ggacttattg atgagtactt ctgataaaaa gatccaacta gcagcagaaa ccccttatat
    16141 agaaggagta catccaaaaa atacttggtt gttcgaacat ccagtccagg cagctgattt
    16201 tccaaaagca attcgtgcat ggacgattaa cgatgaagaa gaaatggcca aatgtcaaga
    16261 actcaaaata gatggaataa taactgattt tccggagaaa gctcttcggt ggaaacaatc
    16321 aacgaaaagt aggtaaaata tggaaaaagt attagtgatc gtcggtccga cagcagtcgg
    16381 taaaacagcg ctaagtgtgg aactcgcaca actatttgga ggagagatca tcagtggtga
    16441 ttcattgcaa atctataaaa atctggatat cggtacagca aaaatcaaag aagaagagat
    16501 gaataatgtt ccgcatcatt tgattgatgt gatcgaacct actcaacgtt actcagccgc
    16561 tgattttcag cattcaggtc gacaattgat taccgagatt gcgaaccgag ggcacctacc
    16621 tatcatagct ggtggtacgg gtctctatat ccaatccctt ctatatgatt atcagttagg
    16681 agcgacagaa gaagcgaatc ctgagattag aaaaaagtat gaaacgttag ctgaaacaat
    16741 tggttcccaa gcattatgga atcagttgaa agaaaaagac ccggcagctg ctgagaaaat
    16801 ccactggaac aatcaacgca aagtgataag agctctggaa gttttagaga cgacaggaca
    16861 tagtatcacg gcacctaaag aagagccaaa gcaattgtat gattacctca tgattggatt
    16921 agatacgaag cgtagtattc tttatgaacg aatcaatcaa agagtcgatt tgatgctagc
    16981 agaaggttta gtagcagaag cgcgtaaggt gtatgagctt ggagaagtcc aggccagtca
    17041 aggtattgga tataaagaat ttttccctta ttttgaaggg aaagaaacat tggaagaagc
    17101 aacagaacaa gtcaaactac attcgagaag atacgcgaaa cgtcagttga cgtggtttcg
    17161 taaccgtttg gatgcacagt ggtttgatct tataagtgag ccaaatcaaa aaagtcagat
    17221 tgagcaaaca atcaaaaaat ggctggaggc ggaataaaat ggaagaaaaa gtgatcatcg
    17281 taggggtaga aaccgaagca aaccagcgtt acttttcaga gtcaatggaa gagctagttc
    17341 agctgacgaa taccgcatca ggagaagtcg tattcacaat tacacaaaag agaccacaag
    17401 tggatcggca aacgattatc ggtaaaggga aattacaaga attagttcaa caagcggatg
    17461 cgcatgaagc ggatttgatc atctttaatc atgagctaac tccgagacag agccaactga
    17521 ttaccgatgc tgttggtctt ccggtaattg atcgcgtgca gcttatctta gatatttttg
    17581 cgatgcgtgc tcgttcgaaa gaagggaagc tgcaggttga acttgctcag ttagaatacc
    17641 tgctgcctcg tttagtaggg caagggaaaa cattatctcg tcttggcggt gggatcggaa
    17701 caagagggcc aggtgaaaca aagttagaaa cagaccgccg acatatacgg aataaaattt
    17761 ttgctgttaa gaaagaattg aaagaagtag aagctcatcg agagcgcaat cgccaaaaaa
    17821 gaaaaaatag cgagattttt cagattggac ttattggcta cacgaatgcc gggaagtcaa
    17881 ctatcttgaa tctattgaca caagcggata cttattcgaa agatcaattg tttgcaacgt
    17941 tagatccatt gactaagaga tggcgttttg ctgaaggatt tgagattact gtaacggata
    18001 ccgtcgggtt catccaagac ctgcctacgc aactgatcga tgctttccat tctacattag
    18061 aagaaagtca aaatatggat cttttgctac atgtagtcga tgctagttct ccagatcgta
    18121 ttttacagga gcagactgtc ttaaaattga tggatgaact gaatatgaaa gaaatgccga
    18181 tactgactgt ttacaataag gcagaccaga tcgacccagc gatgttcacg cctacgctat
    18241 tcccaaatgt attgatttct actcagacga aagaaggtaa gatggcgttg atcgaagcaa
    18301 tcaaacgtca gctgatggaa ctaatgaccc cttatacact ttctgtgcca agcaacgaag
    18361 gacagaaatt aagcgagttg agaagacaaa caatggtttt ggacgaagcg tacatggaag
    18421 aaacaaacga atatgaagtt aaagggtttg caaaaaccaa ttcaaaatgg ttaagaaaaa
    18481 accaataaaa ttttattttt ttaa
//
LOCUS       DACCKU010000058        17848 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.061, whole genome shotgun sequence.
ACCESSION   DACCKU010000058 DACCKU010000000
VERSION     DACCKU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.061"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(79..151)
                     /locus_tag="IVN13_001866"
     tRNA            complement(79..151)
                     /locus_tag="IVN13_001866"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
     gene            532..1101
                     /locus_tag="IVN13_001867"
     CDS             532..1101
                     /locus_tag="IVN13_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP7012338.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1088..1948
                     /locus_tag="IVN13_001868"
     CDS             1088..1948
                     /locus_tag="IVN13_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7012339.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2138..2842
                     /locus_tag="IVN13_001869"
     CDS             2138..2842
                     /locus_tag="IVN13_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7012340.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2847..4682
                     /gene="walK"
                     /locus_tag="IVN13_001870"
     CDS             2847..4682
                     /gene="walK"
                     /locus_tag="IVN13_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP7012341.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4679..5995
                     /locus_tag="IVN13_001871"
     CDS             4679..5995
                     /locus_tag="IVN13_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012342.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5996..6865
                     /locus_tag="IVN13_001872"
     CDS             5996..6865
                     /locus_tag="IVN13_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012343.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6920..7729
                     /locus_tag="IVN13_001873"
     CDS             6920..7729
                     /locus_tag="IVN13_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7012344.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7832..8461)
                     /locus_tag="IVN13_001874"
     CDS             complement(7832..8461)
                     /locus_tag="IVN13_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAP7012345.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8631..9923
                     /locus_tag="IVN13_001875"
     CDS             8631..9923
                     /locus_tag="IVN13_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012346.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9999..10661)
                     /locus_tag="IVN13_001876"
     CDS             complement(9999..10661)
                     /locus_tag="IVN13_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7012347.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10886..11170
                     /locus_tag="IVN13_001877"
     CDS             10886..11170
                     /locus_tag="IVN13_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP7012348.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11221..12846
                     /gene="groL"
                     /locus_tag="IVN13_001878"
     CDS             11221..12846
                     /gene="groL"
                     /locus_tag="IVN13_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP7012349.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(12935..14083)
                     /locus_tag="IVN13_001879"
     CDS             complement(12935..14083)
                     /locus_tag="IVN13_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7012350.1"
                     /translation="MLKVWVEQTKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALENYNKSDITFDELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYSSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRLTDPDQYIFISTSGTPLTLHSFNAVLHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(14185..14769)
                     /locus_tag="IVN13_001880"
     CDS             complement(14185..14769)
                     /locus_tag="IVN13_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP7012351.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(14896..15327)
                     /locus_tag="IVN13_001881"
     CDS             complement(14896..15327)
                     /locus_tag="IVN13_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7012352.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(15345..15665)
                     /locus_tag="IVN13_001882"
     CDS             complement(15345..15665)
                     /locus_tag="IVN13_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7012353.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREEKETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            16397..16654
                     /locus_tag="IVN13_001883"
     CDS             16397..16654
                     /locus_tag="IVN13_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP7012354.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(16625..16933)
                     /locus_tag="IVN13_001884"
     CDS             complement(16625..16933)
                     /locus_tag="IVN13_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012355.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            17011..17757
                     /locus_tag="IVN13_001885"
     CDS             17011..17757
                     /locus_tag="IVN13_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7012356.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
BASE COUNT         5875 a         3080 c         3625 g         5268 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatgaa atgaaaaaag gagcggttat gatggaaaaa
      541 atgcgtcagc aattaatgga gtgggggaag gaactagaaa cattccgtct gcctcactgg
      601 gaagaactcc ctgacttaga actttatatg gaccaagtca gaacgttagt tgatcgttat
      661 ctttcacctg tgatacaagg agaaaagcat ccattgctta cctcttctat ggtaaacaat
      721 tacgttaaac taggtttgat accagcacca gttaaaaaaa gatataacaa agaacacgta
      781 gcatttttac ttgcaatcac tacgttgaaa caagtattga ctattcctga aatcaaagaa
      841 gggatattgt tccaaggaaa aacagtagga attcgtgaag cctataatct attttgcgat
      901 gaacaagaag cagctgtgtg gatggtcagc caattagcac aaggtaaatc tcatccgcaa
      961 aaattcgacc aggcaacccc tgtagaatat attgctgtaa gagcggcaac tttatcgttt
     1021 gcaatgaaac ttcttgcgga aaaaacgatt gttctagaaa ccgaatattt aaaagaggag
     1081 aaaacaaatg aaaaacaata aaatcgcaat tttagtagat tcaggtacgg atgtgccaga
     1141 agaattgata aaaaaatacg agatgtatat gatcccattg aaaattattt ataaagatca
     1201 ggtatataca gataaaattg acattacctc tgaagagatc tatcaacgtt tgccagagga
     1261 aatccctggt acatctttgc cggatggtga gtcgatcaca gaaatctttg agcaaatcaa
     1321 aaaagacggt tatgaaaaag tacttgcggt aacaatttcc agtggattga gcgggacata
     1381 taatgtggtt cgtttaattg cacaacagca agaagaattg gaagcatatg tactagatac
     1441 gaaaaatatt ggtattggtg caggtttttc tgcgatccaa gcagcaaaat ggctggaaga
     1501 aggagtagaa tgggatcaac tgatcagtaa tctgaatgag ttagtcgaaa gaacaaaagt
     1561 tttctttaat gttgcgacat tggaatatct tcaaaaaggt ggacggatcg gtttagttgc
     1621 ttctattgta ggaaccgctt taaagcttaa cccaatcatc tcatgtaatg aagaagggat
     1681 ttattacaca gtaggaaaag ctaggggacg taaaaaaagt ttggataaaa cggttgctta
     1741 cgtcaaagaa aaaatcggta atgcaaaggt cttcaactta gctgttgccc atggggatgc
     1801 gaaagaagaa gcaattgaaa tggaagcgag acttaaaaga gagttcccac aagcaaacca
     1861 aatctacttt ggccagattt ctccggcatt agtagtccac actggtccgg gattattggg
     1921 tgtcggtgta caagttttag aaggataaat caaaaaaagg aaacactgag gtgaaaatca
     1981 gtgttttcct tttttattga agttagaaca aaaaataata aagtattttg cactttcatt
     2041 ttaattttct cttttgtttt ataggcaaag atgaaacaaa agcagaaata cggtataatg
     2101 agaaggtatg acaataatat ataagaggtg gctctttatg aaaaaagttt tagttgtcga
     2161 tgatgagaag ccgatttcgg atatcgtcaa attcaattta gccaaagaag gctatgatgt
     2221 ttatactgca tatgatgggg aagaagcctt ggaaaaagtt gctgaggtag aacctgattt
     2281 gattttactt gatttgatgc tacctaaaat ggacggctta gaagttgctc gtgaggtacg
     2341 caaaacttat gatatgccga tcattatggt aacagccaaa gattctgaaa ttgataaagt
     2401 cctaggctta gagttaggcg ccgatgatta cgtgaccaag ccattttcca accgagaatt
     2461 agttgcacgt gtcaaagcaa atcttcgtcg cggtgccacg gctgcgaagg aaccagaaga
     2521 ggcagcacct gcggaattga cgattggtga tttgacgatc catccagaag cttacatggt
     2581 gacgaaacga ggagaaacaa tcgagttaac acatcgtgaa ttcgaactac tcttttactt
     2641 ggcgaagcac cttggacaag tgatgacaag agaacattta ttacaaacgg tttggggtta
     2701 tgattatttt ggagatgtac gaacagtgga tgtaactgta cgtcgtttgc gtgaaaagat
     2761 cgaagataat ccaagccatc caaattattt agtgacacgt cgtggtgtcg gttactattt
     2821 gcgtaaccca gaacaggagt aattggatga aaagaaaagt ccgctttttt cgatcagtca
     2881 attttaagat cgctattacc tttattctga ttttattgat ctcgatcgaa attattggtg
     2941 cctactttat acgtggttta gaacgttcca cgatcaatac atttattaaa gatatgaatc
     3001 aaacggtgga atcattagca acaacaatca gcccggaatt gaatcgaaaa gataatgcag
     3061 atgatgaaga ggtaaatgcg aatatcaaaa gatttattga aaacagtgcg actagcgata
     3121 tcatcgaaat acgggtcgtt gacgaaaaag ggatcatccg aggaacaacc gatgtcaatg
     3181 aacaaagcgc tgtgggaaag aaaaatgatt atgttgatat caatgatttc acgactaaac
     3241 gttatgttgc tttagataac gacgataaac gggtgaatat caatgttcaa ccgatcctgt
     3301 ccccgaccgg cgatgccgtt atcggggctc tttacgtcaa aagtaatatc gaacaaaaat
     3361 attcggaaat caataacaca gcagtcattt tctttactgc ctcactaatt gcggcattta
     3421 tttcaatgat cgtatctgtt ttagttgctc gttcgattac acagccgatc ggtgaaatga
     3481 gagaacaagc cattcgaatc gctagaggag actacagtcg gaaagtcaaa gtgcatggac
     3541 aagatgaact tggacagctg gctgatacct tcaatcagtt agctgaacgt attgaagaag
     3601 ctcaagatac aatggaagct gaacgcaatc gtttggatag cgtgctatcc catatgacag
     3661 acggcgtcat tgcgacagat cgcaggggta aagtcattac gatcaatgat atggctgttt
     3721 ccttgctaga tatcaaaaac gaggaagcaa tcggacaatc gattttgaca cttttagata
     3781 tcgaagaaga atatacgttg cggaaattat tagaagatcc aaacgaaatg gtacttgacc
     3841 gttcaaccag tacattggaa agtgatcaga tgatcttgcg agtagacttt tctatgatcc
     3901 gtcgcgaatc aggttttatc agcggattag tagctgtctt gcatgatgtg acagaacaag
     3961 aaaaaacaga acgggaacgt cgtgaattcg tctctaatgt ttcccatgaa cttcgtacac
     4021 cgttgacaag tatgcgtagt tatatcgaag cattgagcga aggtgcgtgg aaagacgagg
     4081 aagtagcacc aaattttttg aaggtaacgc tagatgaaac agaccgaatg attcgaatga
     4141 tcaatgacct cttaaatcta tctcgcatgg atacaggtaa tacccagctt caattagaat
     4201 atgtcaattt caacgagtta gtgaattttg ttcttgaccg gtttgatatg atggtgggaa
     4261 atcaggaaaa aaattataag atccgtcgtg aatttacgca aagagatctt tgggtcgaag
     4321 tagatacaga taaaatcatc caagtagtcg ataatatcat gaataacgcg atcaagtatt
     4381 caccagatgg gggaacgatt acttgtcgct tattagaaac gcataataac gtggttttaa
     4441 gtatcaccga tcaaggattg gggattccta aaaaagatct gaaccgagtg ttcgagcggt
     4501 tctatcgagt agataaagca cgtgcaagag cacaaggtgg gacaggactt ggattagcta
     4561 tttctagaga agtgattaag gcgcatcgag gcgctatttg ggcagagagc aaggaaggaa
     4621 aagggtcgac tttttatatc tcgctgccat atgaacctta tgaggaggaa tggtgggaat
     4681 gagaatctct gaaaaagtcg ttcgtatagg actcattttg atggttgcgt taagcatata
     4741 cttctcttat gcaatctggc tgagcccagc aggaaaaagt tcaatcaacg tagatgagac
     4801 aaacactcaa atgatcgagt ctcaaagcaa tcgaaaaact tccgaagtct ttcttccgct
     4861 tcatgttaca tggcaccatt cgggaatgat ccaagaaacc aacagtgaaa atctcgtgta
     4921 ccgattgcaa ttgatcatcg ataaagcaag atttggaaag ctttctgaag tagtttcagg
     4981 agacaaatca cagtttgaac aggtgaaaaa tctgaactct ggttttgagt tggcctataa
     5041 tgcgcctttt ttgctaagag aatacaagga agcatttgat ttagatttag atgtgtcgaa
     5101 cttggcaaaa gaaagcgaaa atatgtattt tactagaata caattcgatc aaggaaataa
     5161 aaaaatccga tttttgaatt attcgaacca tctcgtatat gaagcaaata tttcgttgaa
     5221 ttggacagat atcaataaag aattaacagc aaaagacaca aaatggacag aaatgacaac
     5281 agaaccatcg acgatcgaca cgcaatatgc tacgaaacat tcgatcaaac tgaaaaagta
     5341 tagttatatc ctgtcacagc aaccctatac actttttaga aatgcttttt tccagaaacc
     5401 agatagcgtg aaaaataatg atgaaagcaa tgaattgatt ttttacggtg ggaatgaaac
     5461 aatgtctatc cattcggaaa cgcaagtagt tgattttcgt ggagagctgc cagagaaaaa
     5521 agaaaatgct agtgtatttt ctgaaagttt cccctatatt agcaaattag gcagttcttt
     5581 agggaacctc agatattttg accgcagtga caatacgatc gattatcgga tattcgtgga
     5641 aggcttcccg gtttttggga cagacagcag aggacagatt ggtgtggaag ttggcggtga
     5701 aacgaatggt tctgtgcaag tgaatatcca aaccagtttg aatacgattc aagtgccgat
     5761 accttcagac gaagaagtag aacttgcaag tacgaatgaa atcatccagc agttaaaagc
     5821 taagggagca gatagcaaca agattggttc agtgatcgtt ggctatacat ggcaaaatgt
     5881 caaagaaacg aatcgtgtcg ttgatctctt acctgaatgg tatgtgaaat ataaagataa
     5941 ctggtattca gctaacgacc ttctggaaaa attacctgga ttggaggcga attaattgga
     6001 tttcaaaaaa atcgaatgga tatttttcgt cgctttttta gggttgaatg tgtttttatt
     6061 cagcagttat catgacgcgg tataccaaga aaacaatgtc attcgctcta atcagactga
     6121 atcaatccaa caacgtctgg ctagcgacga tatcacttac acggggaaga tttcgtctga
     6181 gaacaagcaa ggctattatc ttagcgcgga gcagaccaat cttagcactt cattagcgaa
     6241 ttatcgcaag gctggtaaaa gcggcttgct ggcaagtggt gtgtttttag acggtgatgt
     6301 tttgacgaaa tctttatcca gttcagatgc tgaaaagaac gtgatcgatc cagataaaat
     6361 tagtagttcc ttggaagatt ttttatctaa caaagacaat attttattcg gtaatgatta
     6421 tgtgtacacg aaaaactttt ctcatttaga agaagaacca ctagaaatcc aagcaagcca
     6481 agagtatgaa ggcatcccat tcaatgatga tacagcaaaa atcgttcttt ctttagaaaa
     6541 aaacgataat gattatagca tcacgaaata catgcagacg catttatctg atatcgagca
     6601 gctgcgcgaa aagacggaac tgtatacgga agaggaagca atcaatacat tgtacatcaa
     6661 taacaaaata ccaagaggat caaaaatttt atggcgtcag ctagcttatt cctgtattct
     6721 gaaagtccgg gaaaaaaatg tgtatgttcc tgtttggcat gtagcgatcg aaacaagcga
     6781 taaagtggtc caaattgaga gtgtcaacgc tttcagcaat acgatcgtga caaacaatac
     6841 tgtaccaaaa gtggaagatc aataaaaaag cgagtataat gaaaaaagtt tacaaagtaa
     6901 gaagggaagt aggagtcaaa tggctgaaga aaaaccagct tttaaaatca gtatcctagc
     6961 aagtggaagt tcgggtaatt cactttatat cgaaagtgaa aaaaaacggc ttttggtcga
     7021 tgcaggactt agcggtaaaa aaatcacttc cttgatggct caaattgatc ggacaccgga
     7081 ccaattagat ggaatacttg tgacgcatga acatcgagat catatacatg gcgtgggtgt
     7141 cttggctcgt aagtatcatt tggatgtttt tgccaatcag cagacttggg aagcgatgga
     7201 accactgatt ggaaatgtac cagtggaaca aaaacatcta tttgagatgg ggaaagtcag
     7261 aacattcggc gatatcgata ttgagagttt cggcgtctcc catgacgcag cggctccgca
     7321 attttatcgt ttccataaag aaaatcggtc attcgtcatg ttgacggaca ccggatattg
     7381 cagtgattat atgcgaggtg tcattgaaaa tgccgatggt tatttgatgg aaagtaatca
     7441 tgatcttgag atgttacgca tgggacctta tccgtggaat ttaaaacagc gtattttagg
     7501 agatagagga catctttcca acgaagacgg tgcacttgtc atgactgatg tgatcggtaa
     7561 tcggaccaaa cgaatctatc ttggccatct tagcaaggaa aacaatatga aagaacttgc
     7621 tcatttaaca atggaaaatg ttttgaaaga aaaagatttt ggcgttggac atgattttga
     7681 aatctatgat acagatccag atgaagcaac tgagctattt tcgatttaaa cagttcaatc
     7741 aataaaaata taaaaagcag aagataaaat caatcagggt tggttttatc ttctattttt
     7801 atttttttag catattaaaa aaataaccgt attatcgact gactacactg atgtgctgct
     7861 tttcatgttt ctcatccagt atctcatcta ctaatgcaat cgcgtaatct gccatgctga
     7921 tcgtactgtc gccagcagcg tttctttgta aataatcatc acttaacaaa taagtacctg
     7981 tacgtttgcc atgtggatca aataaagaag ctggactgat ataagtccaa tgtagatttt
     8041 tagattgttt caattttaac aatccttcgt acatattgaa agctgttgga tagaagggtg
     8101 catttttagg tacttggtca atcatacgag tcgtcttttc tgtatctaag aataacgaag
     8161 aggcaccccc aacgacaatt aagcgtgtat cggttccttc aagcatctca atcaaatggg
     8221 acaaagaagt ttgatgcatt tcttcttttc ctttgggagc gttgaaagca tcaacgacaa
     8281 catcaaaaga tgcaagatca gtagttgtca actcaaataa atccttttgt ataaacggaa
     8341 catctgatgg caatttttct ggatgacgca cgattgccgt cacgtctaaa tctctggaca
     8401 atgcttcgtc taaaataaat gaacctgcat ggccagtagc tccaatgagt gcaattttca
     8461 taaaaaacct ccttttttat tcctgtccat tgtaagcagt tgctgaatat tcgtcaaaga
     8521 atccgatttt tgaaaagctc agtgcgcatt cctttcatgg ttcgtgatca ttgggtatag
     8581 ttaaattaag aaaactgatt gataaaaata gaagttgaag gagataagaa atgaaagagt
     8641 ggttagatcg tgccttttta ccaatgataa catctgttgt gaatcttaga cccttaactg
     8701 ctttgcgtaa tggaatgact tacacgatcc caataattat tgttggctcg ctgtttttct
     8761 tatttgctca ttttcctgtg gattacatcg ctcagtggat agacaaaaca ggattttcta
     8821 tgtattttac acaagtatac caggctacat ttgatgtgat agccttttgg atcgtttttg
     8881 ccattgcata cgcttatgca aaagaagaag acatcgatcg cttaccagtc ggcataacag
     8941 ccttgtctgc ttatctactg cttattcagc cactaaatac tcagatagat actgtctgga
     9001 caggtgctga agggatacta ggagcagtta caactgggct catcatcgga tggggctatg
     9061 cacgtttagt aagacttgcg aaagggtgga agcaaagaga gaagatacct gaaaatgttt
     9121 ggcagtcttt tacttcattg attccaatca ttgttatagt cacttttacg cttatcggtt
     9181 cagcggtttt tcaacagttg ttcagaatga ctgttgttca aggagtatgg acatatgtcc
     9241 agcttcccat tcaatcgttc atagacacgt tgtttggtgt gcttttatta ggtttttttg
     9301 ttccgttttt atggttattt ggtcttcatg gatctgcgat ggtcaatggg ttggttagtc
     9361 ccgtcttgca agcgaattca ttagcgaatg cagaaatctt agcctcagga caagagttga
     9421 cggtggcaaa tggcggacat atcgttactc agcaatttct cgatcagttc atgacggtta
     9481 cgggtgctgg attgacgtta ggggctgtat tttttatgac ggtttttgcg aaatcgaaga
     9541 aatacagaga attaggaaat cttgctcttt taccagcctt tttcaacatc aatgaatcaa
     9601 ttattttttc cacaccgatc gtgatgaacc cgatgatggc tgttcctttt attttcgcac
     9661 cggtcgtttc aggattgatt acctattccg ctttatattt tggattcgtt cctttattta
     9721 cagcagtcca agtcccttgg acaacaccgc cgatcctctc gggtttctta gtaggcggag
     9781 tgcctgcagc gattttacaa ggtatcgtgt tgtcaattag ttttttcatt tatttcccat
     9841 ttttgaaaaa aatagattct gcaaactgca aaaaggaaag acagacaaaa aatgatggag
     9901 cagcagaaaa gatgaacgac taaaaaacaa aaatactcct gtcgctttag attgggaaaa
     9961 tcaccaatct aggatgacag gagtatttat agtcatgttt atactagccc tttttggaca
    10021 gctaattgaa caagaacaac taatgtgttc attcctacgt gtgtgatcat cgatgtccag
    10081 atccgaccag ttgctttgta ctctaggctg aagaagaaac ctaggaaaaa gtagattagc
    10141 agatgaccat cattatgagc aaacgcaaac agcaaagagc tgatcactgc tccgacccaa
    10201 aaatttgaat atcgactgat gatccccagt atactgcggc ggaatacgaa ctcttccatg
    10261 atgggtccag caacagttgt cgccaaaata aaagcgggtg cttccatgat catctggatg
    10321 atattttgtg tattctcgga aggggttgct ttgccaaaca atacagattc taacatgaca
    10381 gcgatgcttt gcagaatcaa tgcgacaaag attccaacca atccataaat aacaatcttt
    10441 ggagcagaag ctttttttac cgctgattcg atcgctaagg gttcacgttg cttaaaataa
    10501 aaaatagcta ggacaacggc gcctagtata tacatgactg cagttgctgt gatcaattca
    10561 gacgtagatt ttacaagtcc tatcgaagct aggatcaagg gtgagataaa aattagacca
    10621 tagcaaaaaa tgctgaggaa gctatatttt tttaaagaca tgtaattcct ccttttaagg
    10681 gataacaagc atgcaaggca agcttatatc gattgatttc aattatatca taatttcaat
    10741 aagcagctgt cagaataaaa tttcttttct ttgatttaaa agcttgcaaa aaagagggag
    10801 aatgagtatt attaaaaatg taggttagca ctcgaaggtt aagagtgcta acagattaaa
    10861 aaaagagtta tcggagggat ttatcgtgtt aaaaccatta ggtgatcgcg tcattatcga
    10921 agtcgcaaaa gaagaagaaa aaactgtcgg aggaatcgtt ttagcttctg ctgctaaaga
    10981 gaaaccccaa actgggacag ttgttgcagt cggtgaaggc cgtttgttag aaaacggtga
    11041 aaaagttcct gccgctgtaa aagcaggcga ccaagttatg ttcgaaaaat acgccggtac
    11101 agaagtaaaa tacgaaggaa aagaatatct gatcgtcgca ggaaaagata tcatggcgat
    11161 tgttgagtaa atttggaaga taaaaaaata atcaaaaata atcaatgagg tgaatcaatc
    11221 atggcaaaag aattgaaatt tgcagaagac gcacgcgcag ctatgttacg cggggtagat
    11281 aaattagcag atacagtgaa agtaacatta ggacctaaag gccgaaatgt cgtactagaa
    11341 aaatcttacg gctctccatt gatcacaaat gatggtgtga cgatcgcaaa agaaatcgaa
    11401 ttagaagatc attttgaaaa catgggtgca aaacttgtat cagaagtagc atcaaaaaca
    11461 aatgatattg caggagacgg tacaacgaca gcaactgttc tgacacaagc aatcgttcgc
    11521 gaaggtttaa aaaacgtaac tgcaggtgct aatcctctag gcattcgtcg cgggatcgaa
    11581 ttggcaacaa aagcagcagt cgaagaattg cataatatct caactgttgt tgattcaaaa
    11641 gaagccattg cacaagtagc agctgtttct tcgggttctg ataaagttgg acatttgatt
    11701 gctgatgcaa tggaaaaagt tggaaacgac ggtgtcatca caatcgaaga atcaaaaggg
    11761 atcgaaacag aactagatgt tgtagaaggt atgcagtttg atcgtggtta cttatcacaa
    11821 tatatggtaa cagataacga caaaatggaa gctgttctag aaaacccata tatcttgatc
    11881 acagacaaaa aaatctctaa catccaagat attctgccat tgttagaaca aatcttgcaa
    11941 caatcacgtc cattgttgat tattgctgac gatgtagatg gtgaagcatt gccaactctt
    12001 gtattgaaca aaatccgtgg aacattcaac gtagtagctg taaaagctcc tggtttcggc
    12061 gatcgtcgta aagcaatgct tgaagatatc gctattttga caggtggtac agtaattact
    12121 gacgatctag gtttagaatt gaaagatgta acaatcgaaa acttaggaaa tgcttctaaa
    12181 gttgttgtag ataaagacaa tacaacgatc gtagaaggtt ctggagaaaa agaagcaatc
    12241 gaagctcgtg tgcaattgat caaaaaccaa attgctgaaa caacttctga ttttgaccgc
    12301 gaaaaattac aagaacgctt agctaaatta gcaggtggtg tagctgttgt taaagttggt
    12361 gcagctactg aaacagaatt gaaagaatta aaattacgca ttgaagatgc attgaatgct
    12421 acacgtgccg cagtagaaga aggaatggtc tcaggtggtg gtacagctct agtgaacgtt
    12481 atcagcaaag tttctgctgt agaagcagaa ggagacgtgg caacaggtat caagatcgtt
    12541 gttcgtgctt tagaagaacc aattcgtcaa atcgctgaaa acgctggtta tgaaggatca
    12601 gtgattgttg ataaattgaa gaacgtggaa ctaggcactg gtttcaatgc tgctactgga
    12661 gaatgggtaa acatggttga agcaggtatc gtagacccaa ctaaagtaac acgttctgcc
    12721 ttgcaaaatg ccgcttccgt ttctgccttg ttattaacga cagaagcagt cgttgcagat
    12781 aaaccagaac cagcagcgcc agcagctcct gcaatggatc catcaatgat gggcggcatg
    12841 atgtagtaat taagaaagaa ccctatttta acagggttct ttcttctttt tttgttttat
    12901 tttaagtaaa aggggcaaag aaggggcaaa aatatcaaag gctatttagt ttatcaatta
    12961 cttgttgttt ggctcttttg gttacatggt tatagataga cagagttgta tttgcatcag
    13021 aatgtcctac acgttccata atggctttaa gaggtacgcc caattcagat aatagtgaaa
    13081 catggctatg tctaaatata tgcgatgata gacttttttc taactccaat tcttcctcta
    13141 ctttatggag tacagcgtta aatgagtgca gcgtaagcgg agtgccactt gtagatataa
    13201 atatatattg atcgggatct gtgagtctac ccgcaagaat gttgtcagct atcacacttt
    13261 caatcaattc ttttgcacga ttgggcaatt gtacttcgcg ttgcgaataa gtattttttg
    13321 gagttgtttt tatagcatta tccattttca cagatgagta atctaaggta ccgttaatgg
    13381 aaattttccc atcctcatag tccttcattt gtaaagctag caattcccca tatctcaaac
    13441 cagtcaaata taaaaattca gctattatgc cgtgtagttt tctgcgagga ttggagtata
    13501 gctgtttcag tatttgatca atttcttctt tctccagata ttttttatcc atagaaagct
    13561 ttcttttttc ttcttccact tttttagggt ggattttaac tgctagagca gggtttcttt
    13621 ggatgtattt tctatcgatt gcatagttta gcataacaga tagagttgtt tttgtttgtt
    13681 ttgtgtagtt cagtgagagg tcaccaaacg tatacatatc ttcaattatc ttattaatga
    13741 gtgtctcatc tatgtttcta acgattgtat catcgcttat gtgcttagaa acatgtttca
    13801 tcatcattgg caccttcaga tagctagtac gtttaacatg ctgcttataa tattcatacc
    13861 attctttata cagctcatca aaagtgatat ctgatttatt gtaattttcg agtgcttctt
    13921 taattttttt atctagaatt ttctgagctt ttttccacgc ttgtggtgaa ttacttgtaa
    13981 gtgttgtaga tttttttcgt gttttttctg tataaggatc tacatatctt tcaataaact
    14041 tgaatcgccc atctttggtt tgttcaaccc acacttttaa catctcctat catttgctat
    14101 aataggcata acaaatagac ctatataggt ttgttttcta aaagcacgct cttactctgg
    14161 acggtggggc gtgtttttta tttattatac agttgcttta aaaatagctg ctttttcaaa
    14221 tgatactaaa ggagagaagt ggatttctat ttctcccttt tggtttaacg tgaaatgagc
    14281 agttacatcc atttgttttc ctggagcaac agatcccatt gtattttcgt tggcatatgt
    14341 ctctgatttt ttgtcatctg gtccatatac ttccacatct gtacctacag ggatatctga
    14401 atcgccatca ttttttacgg tataagtaat ttttactact tgtgcaggtt ggttttcttc
    14461 aaattgattt ctttcatcag ttagttctac actatttagt gtatattcag catcaccaac
    14521 ttttacagtg tcaccaatct tatagaaggt atcgcttttt tcttctttag aagatgaagc
    14581 agaggttgaa gatttagtta ctttttcgcc accattatca cttgctttat tactatctga
    14641 tccaccattt aaagcagaac caataattat aattaaaatt actgctaata cccaaaacca
    14701 tacttttttg taaaaaggtt tactaacttt atacattttt ccgtcttgac ccataacttt
    14761 ttttgccatt taaatattcc tcacttcttg ttataatata tttgcgatct cagaaatgag
    14821 gtatgagtcc gtgtgccagc acgggctttt ttaatgtttt gctgaaatcg gtttttttaa
    14881 taattcttga tgtattcata catattgcct tgcgtaagaa tattttttct taaaatagca
    14941 ttggcagaca gcataacaag tgtatctgag cttattctga atagaataat attccataaa
    15001 tttttcgagg ttaaactgag attcatcagt cagttcgttc tcaatataga tatttaataa
    15061 aattagaata gctattttat ctgcttctgt ttcaaatttt gagtgaaaag ttgtagaagt
    15121 atcgtacaaa actgaaaatt caaaatgaga ggcgctgaaa tgtgcaagtt catgagataa
    15181 atgaaaggct tctgcagttt cgccatatag attttcattc aaaaaaatga ttctaggctt
    15241 cggataatag aagccaggct ctttcatttc catgtaaact actttcaaat tgtattcgct
    15301 caacatttct ttcaatttca aatacataca acccatcact ccaactattc attttcctct
    15361 aaagctttag caattgcaat cgctttacgc attgtctcct tagatatttc ttttccatca
    15421 aaagaaaaaa cagtatcgtt ttctgataaa tccacatgtt taggggtttc tttttcttcg
    15481 cgccccagaa gatagtctac agagacatcg aaatagtcag caatttcttg caaacgttct
    15541 gtagaggggt ttgagttctt aagtctatat aagacgtttt taccatagcc taggtcttct
    15601 tcaactttat ttaatgattt tccctgtttt ttcgctaact cttttattct ctcaaacgtt
    15661 atcatatcaa tattcctttc gttcaagaaa aaatatttaa ctttatttgt taaaattcgt
    15721 tgactatttt aacaaaaggt gttaatatca ttcttgtaaa caagtttaac aactaaaaag
    15781 acaacaaaaa acactattga ttaataaatg ccaaccgcca agaaagcttt taaaatcaat
    15841 gtttatatgt cttatttaac tatgttctga ttttaacact gtatgttaat ttagtcaacg
    15901 cagtttttta aaaagttgtt aaatttgttt acgaatataa aagaaaggag agaagaatat
    15961 ggaaaaaaca gtctcggcaa aaatcgaaga tttgaaatta gacatactga aacaagcaaa
    16021 agtggcgatg gaacacgcgg tagataaaga agactctgcc atggttgcag ccatagcaga
    16081 gattttagct cacgtttagt catcgttttt acggtcttct ggaagctgac catagataac
    16141 agcataatga atatctaaat aagcttgaac aatttctttt ggcgaaattg attcgccttt
    16201 agtagatact tgtgattcgt gatttagcca tgcaacaact aaatccgcag cgatttcaaa
    16261 cggcgttaca ctttgtgtca gttgccataa agaatttcac aatatgtatg gtcgtggtaa
    16321 taataccaaa gagcaatttg aggaatttat ggaaattaaa aaatacaaaa aatcatttat
    16381 taaaggaggc taaacaatga acaaggaaat gagccaaaca atcaaggtac aaaaaatgat
    16441 agatgatcta acacatggaa ttgatagcca agccgataaa atcatcaagg aattgcaagg
    16501 acaaaaagtt aaggatgcaa aaatgcttct caagactatc aactttgaaa tgaatccaac
    16561 aaaaagaaag ctcgccgatg tgttggaaga aaagttggct tccgctatca acgagcaaga
    16621 attactattt gaaaccgata cttttaacag ctgatgtttt atagatgaca gtttcatccg
    16681 aatctatatc atcgccgaga gaaaagaatg ttgcttttga caaaaagccg ataagaccta
    16741 caagcggtct tgatgttgca agagtagata ccccaatatc accttgcaag gtattcacgt
    16801 aaatttgaga agaatagaac attccatctt tttcttcgat atttgacgca tcatcgtatc
    16861 caacgaccaa agtatcggca aatattttga ttttttctcc atcaagcata gtaattacaa
    16921 attctggttt cataaatttt cacctcctta acaattattt cagcctgtca cactgataag
    16981 gaaattatac caaagaaagg aatgagaaat atgaatacac cacaaatttt taatttcgag
    17041 caaaacgaag ttcgaactat tttagtaaat gatgaaccat attttgtagg aaaagatgta
    17101 gcagacgttc taggatattc gaatccgcaa aaagctattc gagatcatgt ggacttggag
    17161 gataagacgc agaacgattc gttcaccgtc aatggaacag cagttgtttt gattaacgag
    17221 tcaggccttt acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt
    17281 tgggtaacga gtgaagtcct tccaacaatt agaaaaacag gtagctattc aaacgtacct
    17341 caaagttttg cacaagcatt gcgtttagca gcggatttag aagaaaagaa ccaattactc
    17401 gaacaacaaa ttgccgagta cgaaccaaag attagctact tagatacgat tctttcatcg
    17461 acagatacgg tagctacttc tcaaattgca gctgattacg gaatgtcggc aattgctcta
    17521 aacaaattgc taaacgagtt aggtgttcag cataaagtta gcggacaatg gatactttac
    17581 cgaaaacata tgaaccaagg atacacaaaa tcgcacacaa gtgagatacc gaaagccgat
    17641 ggcggcacta aagttgtaat gaataccaaa tggacacaga aagggcgagt gtttatttac
    17701 aacttattaa tcgcagaggg ctattaccct caaatggatt tagaggaaat tggttagaaa
    17761 ggagttttag tatgactgac attgcagaaa tcactcaacg agatagagaa aaaatcaaag
    17821 aatatgtcga aagttcaaag ttcttaac
//
LOCUS       DACCKU010000059        17843 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.062, whole genome shotgun sequence.
ACCESSION   DACCKU010000059 DACCKU010000000
VERSION     DACCKU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.062"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            2..286
                     /locus_tag="IVN13_001886"
     CDS             2..286
                     /locus_tag="IVN13_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP7012357.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(567..986)
                     /locus_tag="IVN13_001887"
     CDS             complement(567..986)
                     /locus_tag="IVN13_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP7012358.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(988..1572)
                     /locus_tag="IVN13_001888"
     CDS             complement(988..1572)
                     /locus_tag="IVN13_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP7012359.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1619..3094)
                     /locus_tag="IVN13_001889"
     CDS             complement(1619..3094)
                     /locus_tag="IVN13_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP7012360.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3275..3586
                     /locus_tag="IVN13_001890"
     CDS             3275..3586
                     /locus_tag="IVN13_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012361.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3701..6037)
                     /locus_tag="IVN13_001891"
     CDS             complement(3701..6037)
                     /locus_tag="IVN13_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7012362.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6179..6847
                     /locus_tag="IVN13_001892"
     CDS             6179..6847
                     /locus_tag="IVN13_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7012363.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            6850..7893
                     /locus_tag="IVN13_001893"
     CDS             6850..7893
                     /locus_tag="IVN13_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7012364.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8130..9011)
                     /locus_tag="IVN13_001894"
     CDS             complement(8130..9011)
                     /locus_tag="IVN13_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7012365.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9170..9898
                     /locus_tag="IVN13_001895"
     CDS             9170..9898
                     /locus_tag="IVN13_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP7012366.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10054..10371
                     /locus_tag="IVN13_001896"
     CDS             10054..10371
                     /locus_tag="IVN13_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7012367.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10364..10972
                     /locus_tag="IVN13_001897"
     CDS             10364..10972
                     /locus_tag="IVN13_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP7012368.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            10988..11818
                     /locus_tag="IVN13_001898"
     CDS             10988..11818
                     /locus_tag="IVN13_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP7012369.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            11956..12444
                     /locus_tag="IVN13_001899"
     CDS             11956..12444
                     /locus_tag="IVN13_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7012370.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            12495..13367
                     /locus_tag="IVN13_001900"
     CDS             12495..13367
                     /locus_tag="IVN13_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP7012371.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13543..13905
                     /locus_tag="IVN13_001901"
     CDS             13543..13905
                     /locus_tag="IVN13_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7012372.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            13914..14666
                     /locus_tag="IVN13_001902"
     CDS             13914..14666
                     /locus_tag="IVN13_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012373.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14680..16482
                     /locus_tag="IVN13_001903"
     CDS             14680..16482
                     /locus_tag="IVN13_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012374.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16759..17799
                     /locus_tag="IVN13_001904"
     CDS             16759..17799
                     /locus_tag="IVN13_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7012375.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5598 a         3098 c         3683 g         5464 t
ORIGIN      
        1 aatgagggaa atttattctt ttgaatcaaa aatctatgct tccgaagtcg gcaagggcgg
       61 ggcatatatt atttttccgt atgatataag aaaagaattc ggtgctggaa gagttaaagt
      121 ccaagcaact tttgacaatg tgccgtatga aggcagtatc gtgaatatgg gcgtgaaaaa
      181 tccagatggt tcagtctgct acatccttgg actacgaaaa gatattcgta aaaacatcgg
      241 aaaagaaatc ggtgatattg tagcagttac agtgaagcaa aaataaaaga ggttgtgaca
      301 aaagacgtgt cacaacctct tgataaacgg tgttcaaaag ccagtttctc ggtagtaagt
      361 caaatttatg caaaaatgtg gaaacccatg tttacaaaat ttttcttact actcgaaacg
      421 aagcggcttt atcacaacct cttgataaac ggcgttcaat cagctgtccc tcggtattag
      481 ctcaaaaacg gcaaaatatg agaagctatt tttccatttt tttctctcat actcaggtca
      541 taacgctgat ttcacaacct cttctttcat aatggttttt caccatcttt ataataaact
      601 ggttctttga tatggaaatc tattttttcc aaccaatcta aaatcttgta acctaactct
      661 tctgacatcg gaaaagaggc gttgatttgt gcattggaga tatggtcaac atggataccg
      721 gaagtgatcg tacaacttcc cggaacggca gttttgatga tcacagctat tttttcggct
      781 aagacatcgt ctttatggaa ccgaccatcg tggctaggga aacgtacagt ttgcatctct
      841 gtatcacgag aaaatgtagt gactgttccg atatgtggtg tatctccgcc tgttaaagtg
      901 atcaataagt ctttaccgat tactttaaca gcagcttgga tcgtgaaacc tagtttggtt
      961 acttcaacta tcgtttcttt ttccatatta atcaccctgc caacgttttg aatgctcatt
     1021 tacaatttca aattgatcca aaagtttcat tgtatggtga ttaacaagat cttggatcgt
     1081 ttgaggctga ttataaaaag caggaattgg tggtatgatt tgcacaccta gtcgtgctaa
     1141 tttagtcaag ttttccaagt gaatggcatt taatggcgtt tcacgaggaa caatgactaa
     1201 ctttcgttgc tccttcattg ttacatcagc agcacgtgca atcaggttgt cgcctaaacc
     1261 aacagagata cctgcgactg ttttcatact tgccggtacg atgatcattc catcggtcaa
     1321 aaatgatccg ctggcgacag cagcacctaa atctttgttt gaataatggt aatcaaacag
     1381 gctttcgaat tcagttagtg ataagtcgga ttccagcttt aagttctgtt ttgcccatgg
     1441 actgatgatt ccgtgtgttt ctatcatcgg atattctttt agtttcttca ctaggttgat
     1501 ggcataaatg gtgcctgaag cgccacttac accaatcacg atttttttct tccgttgtgc
     1561 caattgttcc attttccgca ctccttttta tgatgtcaaa acagttgcag atcattcatt
     1621 atttcaaata tttttcccaa tcttctactt cttggaattt tgcacgtaca aaatgttctt
     1681 tcatatcgaa aggaactgta ccgtcaaaga tagtttttgc acttatccca cggaaacgaa
     1741 tagagccagg attatattct ggtaattctg atgggtccaa tggatggttc ctcattcctg
     1801 ggatcacgat gatatcttta tctccttgga aacgagtgtt gaaagtccaa actacatcgt
     1861 tcatgtcgaa aatatcaaca tcttcatcga ctaaaatgac tgtcttcaat tctttgaagg
     1921 ctgataaagc aagaatagct gcttgacgct gaataccttc atccgcttcg ttttctttac
     1981 ggatttgcat gatggtcatc aatttaccac cacctgcagg aggattgtag acatttacta
     2041 cttttcctgg aatagcttta tcgaccaagg caagaatact tgcttctgta ggaataccgg
     2101 ccatagatac gtgttcttca ctgggaccaa tcgttgtttg catgattgga ttgtcttttc
     2161 ggtgagtaac tgctttgact ttcactactt gtacagctgg attagcatcc ccagtatagc
     2221 caggaaattc tggcattgct tttcctgagt tcgtgttgat atcttcttgc attgtttcat
     2281 ttggcatgat ttctgcttca atgatgaatt cagcacgagc aatacccact tcatcgactg
     2341 ctataccatc aacgatttga ataggttcgt tacgcaaagc tcctgcacac catagttcat
     2401 tgtagccaag tggagtggta ggtggttcga aagtcgtgcc aatggcaatt gctggatcta
     2461 aaccaatatt gatagtgata ggcattggtt tattcaaggc ttcgaattgt ttttggaagt
     2521 gaccaatgtg gcgtccgcca ggcatgatgt acataccgat cgtatcttta tcttctagaa
     2581 ccatacgatg gatcgttaca tcgctcattg ttttttctgg atctgatcct aagactacac
     2641 ccattgtgat gtatggacca gcatcttcag gagtatttgt aggtgcaggc aaaatgttgc
     2701 gaatatcgaa gtctgcatcg gtagctagat gaacgacttc ttgtgctgga gcgtctgctt
     2761 tgtctacttt cacaggtttg attggatttt ctacagcgtc tttcaagaaa cgaccaagtg
     2821 ttcgataatc atggtgcaaa atttcaccta cacgtttacg actagccatt gttccgatgt
     2881 tcacacgtgt gccggggaaa cctttgatat tgttgaaagt catagctggt ccttcttgag
     2941 tcggacgttt tactgtaccg ccagcaccga tataacggta aacaccagat aattctgctt
     3001 caggatcgat ttcaatatct gtttcgtgat attgacctgg aagtgttttt agttcttcga
     3061 tgacttttct taaatcatac ggttgatctg tcatgagaga ttcctcctta gttgtcttta
     3121 cctatctata gtaaaaggtg aaaaagagga aaacaattca acttgttcgg taaagtattc
     3181 tacaatggaa tggatgcgtt tttttgataa tagaataaac tatgatatac ttatcactaa
     3241 aagaaagcgc atacttagtg ttggaggaag gaaagtgaaa aaaatggagg agtcatttcc
     3301 taaagcagta aaagtggaaa atatagcgaa tattttgaaa gttacatttg aaaacggaga
     3361 agtaaaatac gtgaaatctc attggacaga agagataacc gatgccttgc aatttggaaa
     3421 aaaaggaaga ggaaaaagaa aaaatcttct cgcactttct cgtaatatgt ggattgggac
     3481 tgaagtaacg attgaagcag atggaacagt attcataaat ggaaaagaca ggtatacgcc
     3541 agaagagttg tggtacaaag gaaagaaaag tataccagaa ttataaaaaa taattaaaaa
     3601 aaacagcgcg aaacaaggaa catattactt cctcgtttct tgctgttttc tactatttca
     3661 gcgctcttat gactcaaatc taaatgtggt tgtcacaacc ttattctgca cgcaaagcga
     3721 tagcggcgtc ttttttcgct gccattcgtg caggaagatg accgccaagc atcgtcaata
     3781 tagtagagac gatcactaga ataatcgcat ggacaggatt tagttgagca acatttttta
     3841 gatccgtcat gttgtataag atgctattga tagggaacgt agctagccat gcaataaaca
     3901 cacctaatat cccagaagaa atacctaaaa tacacgtttc tgcgtcaaat acgcgagtga
     3961 tgtctttttt acgtgcacct aaagctttta gtacaccgat ttcttttgtc cgttcaatca
     4021 cagatgtata agtaatgata ctgatcatga tcatacttgt tactaaagag atccctgcaa
     4081 aagcaataag gacataagtg atagcatcca tcaatccgcc agtcagttca gtcatcgttc
     4141 cagctaaatc agtataaatg attttatctt catctgattt gcctttattg taatcatcta
     4201 gataatccaa gatcttttct ttgtcttcaa aattgttcgg ataaatcatg atacttgaag
     4261 gcaagctgtc tccacctaaa taactaagca atgtttgttt ggcattttcg tcaacttttt
     4321 cagtagttaa gacatttaca ccactgtctc tttgagcttt gacgatttct gattctttgt
     4381 tttcgttgac gatttgagtc gttaattgat cgctataagc gattcctgga gataagagat
     4441 tcattgtaga agaagatttc acgcgtagga tccccgagat cttcaattca tcagaagcat
     4501 tctgatacat cgcatcataa tctgtattag ggataaaatt cccagttgga agttttgtat
     4561 agtaagtatt gttgtttact aatttgaatg ttgtaccaac gatatcatca aaatcaagtt
     4621 tttcgtcttc tttcacatca aatccaagat tcttcaatgc gttgatatta gtattattat
     4681 tgccgtctac gatcagcacg acatctgttg ctgaggctgg ataacttccg gcaagtaaag
     4741 aataattgtc ttttaaaaag ttttctttgc tagtgtcaag ctgtgtaggg aaacttgaca
     4801 ctcccacacc tgtcattgct gacatcgtac tagataaaga aagagattcc gtatcaggat
     4861 tttgattcga gaagctgaca ggctgtactt tgccatttac atcacgtaaa agattgattc
     4921 ctgtagtacg ggtaaatccg atattattac ttaagttagg atcgatgtct gttacgtaat
     4981 cgacgtatgt ttgatcaatc ttattcgtgt gctgtgcttt atcttcttcg ctgacttttg
     5041 cagtaatcgt tttgctgtct ggataatccg ctttgcttgc acccaatccg gcatcgtcac
     5101 gagtctggtt agtcgttacc ttagaaatag taatcggaaa tttcgccatc gtttcagctt
     5161 gcgtgttatc gatttgtttt tggaatccag aagaaaggga cagaacgatc gcgattccga
     5221 tgatcccaat actggaagca aaggaggtca agaaagtacg tccttttttt gttcggatat
     5281 tgttgaagga aagctttaac gctgtccaaa aactcatctt tgttctgcgt aagttaaatt
     5341 gatcatcttt tggtcgttcg atgtgaggat gggaatctgt taagatctta ccatcagaaa
     5401 attcaatgat tcgatctgcg tattgatggg ccagttcagg attatgtgtg accatgatca
     5461 ctaatttttc tttcgataac tcttggatca attccatgat ctggatactt gtttctgtat
     5521 ctaacgctcc ggttggttca tcgcacaaaa ggatatctgg gtcattggct agtgctcgag
     5581 cgatggccac tcgctgcatt tgtccaccag ataactgatt tggcttttta tgcatatgat
     5641 ttgtcaagcc gactcggtgc aatgcatctt ctgcccgttt tcgtttttcg tctttagaaa
     5701 caccacttaa tgtcatccca agttcaacgt tttcaataat gcctagatga ccaatcaagt
     5761 tgtaactttg aaaaatgaaa ccgatagagt tgtttcggta ggcatcccag tcactgtcct
     5821 taaagtcttt agtagacttt ccgttgatta ccatatcacc ggaatcataa ttatctagtc
     5881 caccaatcac attcagcata gtcgttttac cagaaccact aggacctaaa atcgctacga
     5941 attctttttg tctgaaagcg acagacacac catccaatgc tttggtggtg gtttcgccga
     6001 ctttataata tttttttata tcttttaatt gaagcataat cacccttctc ccgtattgaa
     6061 atattcggta tactaataag tatatcagaa caatgatcgt agtatacgaa ataaatgtta
     6121 actgaagatg aactacaaga aaaaatctag aaatttttca aaaaacagag ggagatcaat
     6181 gaatcgtatt ttagtagtag aagatgatcc gcaattacag ttgctttacc gttctgtgtt
     6241 ggaacgagcg ggcttttcag tcatcaccgc attgaatgga gaggatgcgc tgaaaaaact
     6301 agaagcgaca cgtatagaat taatcattac agatatcatg atgccgaaaa tggatggcta
     6361 tgacttgcta gactcattac gtagcaccag atttgacaca cctgttctaa tgattacggc
     6421 aaaggccgat tttgaggata agaaacgcgg gttcaaatta ggagcagatg attatatgac
     6481 aaaacccatc gatgtcaatg agatgatcct acgtgtggaa gcattgcttc gacgttcaaa
     6541 gatcaattac agcagtagct tgataatcgg acaaacaaaa ttagagctag atgcttatac
     6601 agtgttacaa gatcagcatt cgttgattct gcctcaaaaa gaatttctgc ttctgtataa
     6661 attactgtct tatcccaaca agatcttcac tcgccaacag ttgatggatg atatttgggg
     6721 attagatacg aatacagaag aacgaacgat cgatgtacat atcaaacgtt taaggacccg
     6781 ttttgaaaag acggaagatt ttcaaatcat cacagtacgg ggactaggct ataaggcggt
     6841 tatttaaaga tgaaaaaaaa gctattttca caattatgga tctattttgc tagtattgtc
     6901 ttcatttcga tcttagtgac actgctgtgt tttctcggtt ttattttctt attgtctcga
     6961 cattctatct cgcctgctga atcggctgga aaaccaacgt tatacccgtt attcgtcttt
     7021 gcggggttca gtatggtcgt gggtacagga atatcgattt ttgttgggcg gcgtatcctg
     7081 catccaatca gtgcgctagg gacaaatatg agtctggtag caacgggaga tttttcgatt
     7141 cgaatggatg aacagcagaa agtagcagaa gtacagcaac tgtataaaga tttcaacgtt
     7201 atggtccaag aactcaatag tatcgagaca ctgaggaatg actttgtgtc cagtgtatcc
     7261 catgaattca aaacaccact agcaacaatc caaggatatg ttcaattgtt acaagcccca
     7321 aatctctctg acgaagaacg ccaaatattt ttatatcgga ttatcgaaag tatcacgcag
     7381 ctttcacaat taacagagaa catcttaaaa ttgaacaaac tggaaaacca gcgtatccaa
     7441 ctggaaaaga aagagtatcg attagatgaa caaatcagag aagtaatcgt tttttttcag
     7501 cctaaatggg agaaagaaca gctagaattg gatatcgaac ttgctgcagt aaattacaca
     7561 ggcaatgaag agtttttgta ccaagtgtgg ctgaatatca tggataacgc cataaagtac
     7621 aatcaaatca atggacagat tcatatcaaa ctatttgaga cagccacaga gattgtactg
     7681 gaagtgaccg attcaggtgt aggaatgaat gaagagacca gagatcgaat gtttgaaaaa
     7741 ttttatcaag gagatacaag tcgtcaaatt tctgggaatg ggctaggact ttcactggtt
     7801 aaaaaaattc tagaactgca tgatggacgt attgattata gcagtatcga aggagtagga
     7861 acgacagcaa tgatacggtt aagtaaacaa taggaaaaag aataaaaccc ctaaaaattc
     7921 tattgaaggt ttttaggggt ttttagtcgt ttttttgtga ttagggaaat gttcttacgg
     7981 ttcttttggc ataaaaatcg ctgctttctt attgaactac gagaaatgtt agaggtgtca
     8041 ttgaaacaag gacatgatgc aagaaagaga gcgaaagtca tgcgtcgctc tctttttttg
     8101 ggtggataga tggacagaaa atgtagggct tagttttctt cttgatttaa atactcccaa
     8161 aatgtatctg aagccaaaga atgctcaccg actttacgag taaatgcaag ataactctct
     8221 gttgttggag aaatagggag aatcacagta ttttctttta atagattctt gactgttttt
     8281 tccataacaa tagcaatccc catgtcattt gcaatcatat tgatgatcaa gtcgattctt
     8341 gctcctttat aagagatttt tggttcaaat ccagcttcgc gacacagatc tagtacaggt
     8401 tgatacaagt tcgtagtggg gccaaggagc aaaaagttct cctttttcag ttcgcttaga
     8461 tccaatttct ttttactagc taaaggatga tttttaggta gaactgctac aaaatcgtct
     8521 gtttcagtag gcaaccactc aatcgaactt ggtggggcct ggaaacttct agcaaagtgg
     8581 atcgtgtcac tatgttcatt ggcgtcattg taagttaatt cgacactttc cacttctttc
     8641 aactggactg taaattcagg atggcgtttc aaaaactcgg tgattttgga aaaaccttta
     8701 taattgatca ttgtcggtat cgttaagata tttaatgaaa gattgttgtc tttcgtatag
     8761 gtttcaagca cttcttgcat agcatggtat tgttctacga cattttttac atatgggagc
     8821 aataagcttc cagcttcggt caattcgatt tttctatggg cgcgttcgaa taatttgacg
     8881 tctaattctg tttctaacga aagaatctgt ttagaaatat ttccttgggt ggtgaattgt
     8941 cgatcagcag ttttggtgaa attcattgtt tcggctaaat caagaaaaac ttcgaattta
     9001 tggatatcca tcatattcct cctacttatt ccaataaaga atactttaac acaaaaaatg
     9061 aattggtgta atcttctctg tctgttacaa tctttcttgc agcgaaagca ataattacag
     9121 aaatttttct gatttttttc gcttcgtaga aaaagagagg agttttttga tgccaactgg
     9181 agttttgatc aatgtaggtt ccgttttgtt tggcggacta attggcggat tagtaggaaa
     9241 taaattaagt gaacatttta aagcacagct gacgatggtt tttggtgtgt gttcgatggg
     9301 gatgggaatc tattccattg cgccgatgaa aaatatgccg gctgttattt ttgctttagt
     9361 gataggaaca gcgattggtt tgattgttca tttaggtaat gggataaata agggggctgc
     9421 gctgatgcaa gtacctattt ctaaaatctt ccctagtgaa aagcttggta tgacccacga
     9481 tgaatttatc agcacattag tgacggtcat tgttctattt tgtgcaagtg gtacggggat
     9541 ctatggaagt ttagattctg ggatgacagg agacagtacg attttgattt ctaaatctgt
     9601 ccttgatttt ttcacagcag ctatttttgc gtgtaattta gggtatgtcg tttctgtgat
     9661 agcaattccc caattcatta ttttttatat tttgttctta ttggcaaaat ttatttatcc
     9721 attgactaca ccagacatga tcttagattt taaagcttgc ggcggattct tgatggtcgc
     9781 taccggtttt agaatgatca acgtgaaaat gttcccagta gctgacatga tacctgcaat
     9841 gatcgttatt atgccactga gctggatgtg gacaaattgg attatgccac tgttataata
     9901 aaaatcaaag agaagccatt ttttgctgac taaaaaggat caaatctttt agtcagtttt
     9961 tttgtatttg acaaactgat tttgaaacta tattatatgt tgtataatat agattgaaat
    10021 aaaatattac acttgagaat agaaggtgac gtaatgcaca tccagattcc aactgttctt
    10081 cttgatggta ctgtgctagc agcattaagc aaggaagata tgtatgggta tgccttaaca
    10141 aaaatcgtac aagaaagtct ctctgtcagt gaatctacac tatatccggt tttgagaaga
    10201 ttgaaaaaag aaggatatct tgagacctat gatgaacctt ttgaaggcag aaaccgtcga
    10261 tattactcgt tgacaccaat gggaagtcgt catttagaag aaattattga tgaatggatt
    10321 gtttttcgag attctttaga ccggttattg gaggagagca caaatgaatg aatatttgaa
    10381 tgaagttgtg gatcatcttc aagaaattcc tgaagacgat cgttttgatt tgatccagta
    10441 ttatgaggaa tattttttag attctggaaa aacattagat cagatcattg atgaatatgg
    10501 tacaccaaag cagtttgcct taaaattaaa aatcagttac ttttcagaag aagaacaagc
    10561 agaaaacgag ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt taatcattat
    10621 cggtttattt gcttctcctg tcctcattcc actagcactg gggtttatat tggttattgg
    10681 gggactattg gttgctttac tagcgctcat ttttagtatc tatgttttat gtatcagtat
    10741 cttaggtgtt ggcctattca tgatcatctc aggaatcagt gtgtttggac aatcgattct
    10801 gagtggatta ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag tatttatttc
    10861 gccactgttg ctgaaaggaa caaaagggct tttttatgtc atgatgaact ttgttaaatg
    10921 gatgggacga cgttttatta caaaaagaga tctgcattca gcgggcgttt gaaggaggac
    10981 acataaattg agattgaaac attttttgat taccggtatt ggtctgatgc ttactggtgg
    11041 cctcctagca ggtgcaactt atacattagg cgctcaaaag agtctgactt gggataatgg
    11101 tcctcggttg atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa acaaaatcgt
    11161 catccatgca gaaaatcaaa cagtttcggt catacgcgga ttggattttg agataaaaac
    11221 gacttatgat aagacagaaa agccagaaat aaaggagaaa aatggcgtgt tgactgtgaa
    11281 tgcagtcaaa aaatcaacag gtgcaatcat tagtttagaa gacacaagtg cacaaatcac
    11341 tattgttgtc cctgcagatc atgaattatc tgaattgaca atcgaaggga ataatagtgc
    11401 aattgatatc aacgaagtat caatcaaaaa agtgaatact aagacacatg atggctgggt
    11461 atcgttcaat gaatttacag gaggaacaat aactgcgaaa gatgaattag gctcacttcg
    11521 attagatgag gccaatgtgg ataacttgat cgtcacaggt gaaaacagca acttccatct
    11581 agaagacagt ttgatcagca aagaaggaaa agtcatccta aacggtggca atttatcttt
    11641 gcatgagatg gaaaacagtg gctatactat ccaaacgtct ggtgaatctt atattgaaaa
    11701 agactaccag ccaataacaa atactttgtt agaacaagga cagaaaaagc taaagatcac
    11761 tgctaaagac acatcgatca atattacgac ggatcaagag gacaatgaaa cagattgatc
    11821 ctgtactcaa ctggatattt taagaaaaaa cagcaaagat ccttccaatc tttgctgttt
    11881 ttcatcgctt aacgcacata actatagtaa aataagaaag aaaatatcta aaaagaaaac
    11941 agggtgaaag agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa aacagaaatg
    12001 gaaagattac atacttatat cactcaacta agccaaacct tctatgatcc agacaaagaa
    12061 aaagtgatgg ttaattaccc gaacaattca gaaggtcgcc aattagagca agtgtaccat
    12121 gaagttttca aacatttatt gacggtgaag aaagagctgg attattattc tttaccgatt
    12181 atcgatacag gaatattgaa gtatgatggg aagaaggaac gatttatctt taaatctgtt
    12241 cgggaagatt tgcctctttc agcagggatg gatctagagg tcttggtaga agactatttt
    12301 acagaagaaa agcactgggt tcgaacaaaa ttggattatt taccgcaggc agccggaggg
    12361 acacaggcaa gcggttggta catcactgaa gacaaagagt tagaactaga aggtgtaatg
    12421 gcgcgtattc gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa ggataaagga
    12481 gcgcaggaag tagcatgaga gaaaaaagag cattttatat caatggttat gcagggattg
    12541 tcggattggt cgtattagct ttagttggat tgtttttctt ctatttaggt atgtggcaag
    12601 cgaaagtttg ggcacttttt ttaagtattt ttctttggtt gatcacgtta ttgttgttaa
    12661 gttcggcaac tgtagtcagt cctaatcaag caaaagtaat tttgtttttt ggacaatatt
    12721 taggtacgat tcgagaaaat ggctttttcc ttacgatacc tttggcacaa aaaatgactg
    12781 tttcgttgaa agttcggaat ttcaatagtt ctgttttaaa agtcaatgat ttggatggaa
    12841 accccatcga aatctcagca gtagtagtct ttaaagtcgt tgatacagca aaggcattat
    12901 tcgatgtagc gtattatcaa gattttgtag aaatccaaag tgagacagcc attcggcata
    12961 tcgctagcca atacccgtat gatacattca atgagaatga tttgacgctt cgaggaaaca
    13021 cgacggcagt gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca gtagcaggtg
    13081 tcgaagtaat cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc gctagtgcca
    13141 tgctccaacg tcaacaagca aaagccattt tgtctgctcg acaaaccatt gttgaaggag
    13201 ctgtcacaat cactcaaatg gccttagagc aaatcgaaga tggacaagaa atcaatttca
    13261 ccgatgatcg gaaagtccaa ttgatcaata atttacttgt ctcaatcatt acagacaaag
    13321 gaacgcaacc tgttatcaat acaggcgaca tgaaagaccg taagtgacat actttttgta
    13381 agataggaag aaggagttta ggacggatta ttgtcttgaa ctcttttttt agttgtttta
    13441 aagaaaatga caaaattgaa agaagaatga tagttgattc gaaggcagaa ttgttcgctt
    13501 ttttctataa tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa acttctgatc
    13561 ggtctacttg ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga ttacgtagtt
    13621 atgggtggag aagaatacta tgtacaaatt acgacagatg gtacaaaaac agtttcaaaa
    13681 tcagactctg gacaagaaac agtcaactat agatatatcc tgcctggcta tacgaaagag
    13741 ggtaaggaaa aagaactgga cttcaaggga caattgactc gaccgcttcg taaaggagca
    13801 tatttaaaaa tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga agtcgaaaaa
    13861 aaagacgtgc cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa gaaatgaaca
    13921 aaatagtcga agtacagaat gtaacaaaag tatacggaaa aaacaatgag aaaaagacgc
    13981 aagcattaag cggcatcagc ttcgatgtag aaaaaggcga atttattggg atcatgggcg
    14041 cttctggttc tggtaagtcg acacttttga atattttatc cacattagat aaaccaacag
    14101 atgggcatat ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat caactagcag
    14161 attttcgtgc gaatgagatt ggctttattt tccaagattt caatttatta gaaagcttga
    14221 cagcacaaga aaatatcgct gttcctcttt ctttacaagg cgtcagacca aaagaaatca
    14281 agcaacgtgt ccaaaaaatt gcagaacgtt tatccatctc tcatattttg gaaagctatc
    14341 cttctgagat ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca ttgatcacac
    14401 aaccaacgat tctattaggg gatgagccta caggtgcgct ggattccaaa agtgcccggg
    14461 atcttttaga tacgatggac gaattgaata caaaagatca tgtatcgatt cttcttgtga
    14521 cgcatgatcc gttttctgca agttattgcc aacgtatttt attcatcaag gatggcggta
    14581 tccatcaaga agtgaaacgt ggagatcaaa gccgtgattc cttctatcga gaaatactat
    14641 ctattcttgg aaacttggaa cagtaggggg agattgacta tgctttggaa attatcactt
    14701 actgggatca agagccgttt aagagactac ttggtgttat tctcaggatt agttatggca
    14761 tctgctattt tttacatgtt tgaatcaatg gcaagcaacg aagctttttt gaaaagtaat
    14821 acgatcgttg cggctacagt tatcattttc cgattcggtt ctgttttact agggatcatc
    14881 acattcgttt atattttata tgcaaattcc tttttgatga cgatgcggca aaaagactat
    14941 gcaatgttta tgatgctggg agcaaaaggc agaaaaatcg cacagatgat tttttctgaa
    15001 acattcttag tagggatcgc agctacttta gtgggttcag ctgtcggtgt aggattgaca
    15061 agtatcgtcc accgattatt agtggatcag ttagatctca atatttcgca ttttacacct
    15121 tttagtattc acgggttgtt gatcactgtt ttgttttttg ctgttttgtt tttattagct
    15181 gctgttgtca atgctttttc gattgtcaaa aaaccgattt tagcattgat tcgtgcggat
    15241 cagacaccaa caagaatcaa gcagcataaa ttcttgtttt tccttgaagt agtactggga
    15301 attgcttttc taggctgcgg ttactatatg atgaaacacg tggggaccta tcaagtcttt
    15361 ggtatagggg tagccttagt tacgatcgta ttaggaactt atttcatttt ccattctgtg
    15421 atcattttct ttttatcatt actaaagaaa tcagaacaga tttcattgaa aaaattgaac
    15481 aatttcacat tgtcacagtt aaactttcgt attcgtgaat atacacaaat gctctcaatg
    15541 gtcgctatgc tgtttgcctt ggcactgggc gctttgacgg taggattagg ttttaaaaat
    15601 gaaattgcag acttgacaga aaacaattct ggatatgatc tagtgatgaa taatcctcgg
    15661 gcagaagatc agcaaaaaat caaagaactt tcaccaacac tttctgcttc ttatactcaa
    15721 aaagaagatg cgggaacgat ttactataat gcaagtgaat ttgcggctca accattgatc
    15781 atgataaaac atgaagatgg aacaccgccg aagatcacga aagaaaaagt tcctgctaaa
    15841 aaaatgaatg aactgactgt acaagaggaa cttcgaagct atgaattgcc ggaacagcaa
    15901 gaaaaagaaa tcaaggtagt cagtcaatca gaatttgatc aactgaactt gccacaatcc
    15961 actttacagc tagttcaggt aaaagacttc caagcaaacc tgaaaccaat cgaaacactt
    16021 gttgctcaaa ataaaatgaa caatccatct ttgcaaaaag tagagtacag caaccagaaa
    16081 tatgaaatgt atgaagtgta taatggtcta ttctctggtt ttgaatttat gggattcttc
    16141 ctaggtttag ctttcttgac catgcttgct agctgtttga tgttcaagat cttgtcagga
    16201 agcaagagtg acattgctcg ttatgaaatg cttgaaaaga taggaacacg acgcgggttg
    16261 cttaaacaat cgatccgtag agaagtaggc gtgttattct tagcaccagg aatcttaggt
    16321 gtagtccatg tcttattcgg attacaaatg tttggtctat tcatgcaaaa tccatacaaa
    16381 gatatttggg ttccgtttac gatattcttc gtattgtatt tcatctacta tgtactgacg
    16441 acatggttat atactggaat cgtattgaaa aagagattgt gaaaaagctg tcctgcatca
    16501 agtaataaga acagccaagc aaaaatagct cctcatattt ttcgtttggt tgggcttatt
    16561 accgcagatg caagcttttg aacaccgttt gttcggaaaa agaggttgtg acaatatttt
    16621 tgtcacaacc tttttttcac ggtcttttat tagaatatag taaatttttt ctcgttactc
    16681 acggaataag ctgttaagaa aacggtttcc atgtatactg gaatcagtgc aaatgaaaga
    16741 aaagagggtg caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa ctatcacttg
    16801 attacgatca cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg ggcacgggtt
    16861 gttcattttc aaacaataat cgataatgaa aaacgagagt tgatcttagg ttttgatgaa
    16921 gcaaaagaat atctagaaaa agacccttat atcggtgctt caatcggacg tatggctgga
    16981 cggattacga atggcacatt caaattagat gatgaaacgt tcgaagtaaa aacagacccg
    17041 gacactggtc attgtttaca tggtggtgca cctagctttg aagccaagca gtggcattat
    17101 acagtcatca atggtgaaaa cgaagcatcg gtcattttct atcagactag cccagatatg
    17161 gaaaacggat ttcctggaac tttagatgta gaagtacggt atacgctgac gaacgatgat
    17221 acatggcgag tgacggtcca agggatcagt gaccgcaaaa cagtattcaa tcctacaaat
    17281 catgtctatt tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca tatactttgg
    17341 ttgaatagcc gattatttgc tacgctcaat aaagatacaa cgcctacagg agaagtagtt
    17401 tcagtaagtg gcacagcatt tgacttccaa cgtcctaaaa aattaggtga ggtctttgcc
    17461 agtgattttc gccaatcaat gatagtgggc ggcatcgatc atccattttt gttgaaaaaa
    17521 caatctggtt tactaaatgc tgcggaattg acaagcccag atgaaaaagt atcgatccaa
    17581 gtaaaaacag atgcttcagg aatcgtgatt tttacagcaa attttggaga agatggaccg
    17641 gaaatgcgcg ggaataaatt agtaaatcat ggcggtatca catttgagac tcaagaactg
    17701 ccaggagcgg agcgacttga aagttttgga gatgtcactc tggaacctaa ccaagtacgc
    17761 gagtttgtaa cggaatacaa aataaatata aagaaataga ataaattaaa aagggagtgt
    17821 gacagtgtca catccccttt tta
//
LOCUS       DACCKU010000060        17673 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.063, whole genome shotgun sequence.
ACCESSION   DACCKU010000060 DACCKU010000000
VERSION     DACCKU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.063"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(299..1756)
                     /gene="ascB"
                     /locus_tag="IVN13_001905"
     CDS             complement(299..1756)
                     /gene="ascB"
                     /locus_tag="IVN13_001905"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP7012376.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(1753..3648)
                     /locus_tag="IVN13_001906"
     CDS             complement(1753..3648)
                     /locus_tag="IVN13_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7012377.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(3706..5481)
                     /locus_tag="IVN13_001907"
     CDS             complement(3706..5481)
                     /locus_tag="IVN13_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7012378.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(5531..7027)
                     /locus_tag="IVN13_001908"
     CDS             complement(5531..7027)
                     /locus_tag="IVN13_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7012379.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(7221..8843)
                     /locus_tag="IVN13_001909"
     CDS             complement(7221..8843)
                     /locus_tag="IVN13_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP7012380.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(9171..10484)
                     /locus_tag="IVN13_001910"
     CDS             complement(9171..10484)
                     /locus_tag="IVN13_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7012381.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(10729..10953)
                     /locus_tag="IVN13_001911"
     CDS             complement(10729..10953)
                     /locus_tag="IVN13_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012382.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(10973..11341)
                     /locus_tag="IVN13_001912"
     CDS             complement(10973..11341)
                     /locus_tag="IVN13_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP7012383.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(11537..12247)
                     /locus_tag="IVN13_001913"
     CDS             complement(11537..12247)
                     /locus_tag="IVN13_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012384.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(12265..12762)
                     /locus_tag="IVN13_001914"
     CDS             complement(12265..12762)
                     /locus_tag="IVN13_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012385.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(12799..13251)
                     /locus_tag="IVN13_001915"
     CDS             complement(12799..13251)
                     /locus_tag="IVN13_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7012386.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(13386..13991)
                     /locus_tag="IVN13_001916"
     CDS             complement(13386..13991)
                     /locus_tag="IVN13_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP7012387.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(14441..15061)
                     /locus_tag="IVN13_001917"
     CDS             complement(14441..15061)
                     /locus_tag="IVN13_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7012388.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(15063..15932)
                     /locus_tag="IVN13_001918"
     CDS             complement(15063..15932)
                     /locus_tag="IVN13_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP7012389.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(15955..16362)
                     /locus_tag="IVN13_001919"
     CDS             complement(15955..16362)
                     /locus_tag="IVN13_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7012390.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(16381..17670)
                     /locus_tag="IVN13_001920"
     CDS             complement(16381..17670)
                     /locus_tag="IVN13_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012391.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
BASE COUNT         5144 a         3732 c         2900 g         5897 t
ORIGIN      
        1 atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag
       61 ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt
      121 attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca
      181 gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt
      241 ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt
      301 ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta
      361 accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt
      421 ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa
      481 ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc ttcaaatagt
      541 caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc
      601 cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc
      661 tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa
      721 ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga gcactgactg
      781 cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt
      841 catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg
      901 ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa
      961 ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca
     1021 tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg
     1081 ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt
     1141 caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca
     1201 tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa
     1261 actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt
     1321 cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt
     1381 gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc
     1441 ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt
     1501 cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt
     1561 tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt
     1621 tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat
     1681 ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt
     1741 ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt
     1801 cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt
     1861 cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt
     1921 acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt
     1981 atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg
     2041 tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt ttccgtccgt
     2101 tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc
     2161 agataaatca attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc
     2221 ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc
     2281 taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa
     2341 aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa
     2401 tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata
     2461 aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt
     2521 tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac
     2581 ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat
     2641 tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat
     2701 aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag
     2761 ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga ccatcacgat
     2821 gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt
     2881 aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata
     2941 ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact
     3001 gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt
     3061 gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc
     3121 tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca
     3181 taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa
     3241 aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt
     3301 tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt
     3361 tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc
     3421 ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa
     3481 ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat
     3541 atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc
     3601 aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc
     3661 ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc
     3721 actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa
     3781 aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat
     3841 ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag
     3901 cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat
     3961 ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc
     4021 aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa actcatctag
     4081 aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa
     4141 aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat
     4201 ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa
     4261 aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa
     4321 cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac gtaattgaaa
     4381 atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt
     4441 ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat
     4501 atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg
     4561 aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt tttcatcatt
     4621 aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt cttgattgga
     4681 aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca tcggaataaa
     4741 taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag
     4801 ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg atataagaac
     4861 ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac tgatttgttg
     4921 aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga aaattctttt
     4981 tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca gttctaattt
     5041 aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga
     5101 aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa acaataagta
     5161 cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt tcgtcacctc
     5221 ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac ctgaacgttt
     5281 atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga tttcagtttg
     5341 gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta tccaatgatt
     5401 cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca tttgcttaca
     5461 cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt cacttgcaga
     5521 ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta gactggataa
     5581 tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg ctcccatcat
     5641 tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata
     5701 cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct
     5761 cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat
     5821 tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt
     5881 cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac
     5941 tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag
     6001 tatctacgcg catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa
     6061 tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt actggtgcac
     6121 agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg cacatgacat
     6181 cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac
     6241 agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg
     6301 gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg
     6361 cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga
     6421 atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg
     6481 tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa
     6541 aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg
     6601 tttcataatg actaagagta attactggct cgatatcata tcgatttaat gtattgatta
     6661 gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata
     6721 ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa
     6781 tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt
     6841 cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt
     6901 tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac
     6961 cttcacattg attagcagct actgctccgc cccataaaaa attttctgga aatatttcct
     7021 gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca
     7081 tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct
     7141 tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt
     7201 taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa
     7261 tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct
     7321 tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc
     7381 tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca
     7441 tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata
     7501 taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga
     7561 ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc
     7621 ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat ttcctctttt
     7681 gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc
     7741 gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg
     7801 gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca
     7861 tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta
     7921 ttccatcctt catcacctaa agatgtttgc catttggata atactttttt cagttcagag
     7981 atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt
     8041 tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt
     8101 tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct
     8161 tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc
     8221 ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc tttatcgatc
     8281 cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca
     8341 ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccat
     8401 gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc
     8461 caaatataat agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga
     8521 tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt
     8581 gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga
     8641 taattactga tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa
     8701 attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct
     8761 ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa
     8821 tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc
     8881 ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca
     8941 ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt
     9001 tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt tataaacggt
     9061 gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag aaactatttt
     9121 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
     9181 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
     9241 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
     9301 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
     9361 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
     9421 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
     9481 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
     9541 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
     9601 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
     9661 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
     9721 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
     9781 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
     9841 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
     9901 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
     9961 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    10021 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    10081 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    10141 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    10201 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    10261 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    10321 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    10381 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    10441 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    10501 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    10561 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    10621 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    10681 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    10741 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    10801 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    10861 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    10921 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    10981 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    11041 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    11101 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    11161 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    11221 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    11281 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    11341 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    11401 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    11461 aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga atgccgctct
    11521 tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt gtgtgtctaa
    11581 cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc
    11641 cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct tgtgtttctt
    11701 ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc gctccttgtt
    11761 catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca cctaaaggca
    11821 ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc ttttcgatat
    11881 cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata tctacaagcg
    11941 gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct tcaatagaag
    12001 aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga gcaatgactt
    12061 cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct gcctcattca
    12121 ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata ccatttacgg
    12181 ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc actattactt
    12241 ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat agtctgtttg
    12301 gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt ttttacgata
    12361 aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg ttttttgctt
    12421 ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt gatgtttaaa
    12481 tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt cctcagattg
    12541 actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac tcactaggaa
    12601 aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt
    12661 caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg taaatgtttc
    12721 gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa tttcgtattt
    12781 attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa aattccaatc
    12841 ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg cttcttcgtc
    12901 agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga tgaaaggata
    12961 gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt ctttatcttc
    13021 caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag tcgttgtacg
    13081 atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt cgcaaatacg
    13141 tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga tattgctaat
    13201 agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca tttcatccca
    13261 cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa
    13321 taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt cttttacgaa
    13381 aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg tttcttcgtt
    13441 gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat gttggaagtt
    13501 tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa aacaagttta
    13561 taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac tgctcgcaac
    13621 tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac attttcttca
    13681 ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt ccgtttatct
    13741 tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac ttttggtgtt
    13801 ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc ttttcctatt
    13861 tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt atcaaataca
    13921 tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta ttgagccaac
    13981 ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa tcaagtatag
    14041 aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata aaagaatgtt
    14101 aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa tggcgttcaa
    14161 aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc tattcttgtc
    14221 tggctgttct tattaattga ggcaggacag ctttttcaca acctccccac taaacggtgt
    14281 tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaga agctgttttt
    14341 ttgaaattgc tccttgtgac tcggagctga acgacaggct cacgacctct cggtaaacag
    14401 cgttcaaaag ccaatttttc gatattaagc catttctttg ttacttattg tcattgattt
    14461 ctttttctaa tttatccaat agtggagtgt aatccacttg agtatagtct gttacatcaa
    14521 attcatatag tgttcctaac tggaatttgt catattttcg gtcggcgatg atctttttga
    14581 atgcttcttt cgtaatatga ttggtccctt tcgttcgatc ttccaatacg tcaccatagt
    14641 gataatgatc catgatatag ctcaaatgac gatctgattc acgatcacgt ttgtaccgac
    14701 gttgccataa cacttcaaag tctgctgtta ggcgaatcgt gatcacctga tatccgtatc
    14761 tatctgctaa ttcttggagt cgtgcttttt gcttatcact aaaaggatac tcggacacaa
    14821 tcactttctt tcctgcttcc atatacaaat caagtgcttt gtaatagaaa ggataaactt
    14881 tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa cccgatggat tctgcatagt
    14941 attccttgat ttcatcgagc gtgatggcat acatatctgg aaatcgttcc cgtagtttat
    15001 tcataagata tgtttttcct gtaccaggac taccagccag aagaatgaaa tactttttca
    15061 tctcatttat ttcctaagtt aattggactt tccaagttca taaacagtcc aatcttatgc
    15121 ttgacaattt cttttactgc ttcatttgct ggaatcacat tatggcgaag agacttgttg
    15181 atttctgttt cactaaaggt ttctttagct actttcgtcc agttgactaa aagctccgtt
    15241 ccaacattga atttgcggat cccgttattg atcaattcag gataatcttc ttctttcaca
    15301 cctgttcctc catggataac tagggggaca tcaactactg catcgatttc tttcagcaag
    15361 ggaatgttta cttctgtttt tgatttgaat tgtccatgat tcgtaccgat agcaatagct
    15421 aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt cttccggtct tgtatatacc
    15481 ttgtcatttt cagcgacatg gattccttct tctgtaccgc cgattgttcc aagttctcct
    15541 tctacggaaa caccgtgcgc atgggcatat tctactactg cttttgtctt caggatattt
    15601 tctttgaaag gcagatggga cccatcatac atgacagaag tatatccgct gtcgattgct
    15661 tctttgatat catcaaagtc tcgggcgtgg tctaagtgca agactgcatc aaccatttct
    15721 tcttctgcaa tagctgtcat tacattgact aacacatgca tgccgatata tttagcggta
    15781 tcgacacttg tttgaataat gatcggcgaa cccatttctt ttgctgcgcc tagcatagca
    15841 ggaagcattt ctaggttatg cgtattaaaa gctccaactg taaaattcaa ctcttctgct
    15901 gtttttgtta cttctttcaa tgttgtatac atcttttatt gccaccttat ctttttattt
    15961 gtttcctttg atcgttactt gttttgcaat ttgtcgcatc tcatccattt tgcccatgta
    16021 gtaatcgatc ccttgatcat caccagcacg agcagcatct gcacgcttct ggttatagag
    16081 gctcatcagt tttttatacc catctccgat cgtctgttta ttttccaaac tcttttctaa
    16141 atattggatg gcttgatcgt tcacatacaa ttcagaaaat ttaagtccaa cttgttcata
    16201 aagtttcgct aatttatgct tatcttgggt agtggaaatc tcttgtttca attgtgtaat
    16261 ctctgattga atcttttcgg tcgcttctgg cgatagggct ttttcttctt ttgccagaac
    16321 ttttttcggt tctttttctt ttttcttaaa aaatccaaac atcattttcc ctactttcaa
    16381 ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat aagagtgctg cggctactgt
    16441 atctacatcg gttgctgtag cattgttgaa tcccatcgaa ttaaagatcg ttactagcaa
    16501 agcgggcaat aatgtaataa agaaaccatg agcaataccg ccaatgatcg ctcctcggcg
    16561 tccgccaact gcgttgccaa atatccctgc tgtccctccg gcaaagaaat tggttagcat
    16621 accaggtaaa atcatcgcta agccgaacat gggcaaagta aacatggcaa taatcgtacc
    16681 aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg ctataaggaa agaaaactgg
    16741 acaatctaag gcagggatcg catcaggaac taatttcata gctatccctc gaaatgccgg
    16801 cacgatttca cctaacagta gacgtacacc agctagcaat acatacacac ccactacgaa
    16861 ttggatggct tgtaaaaagg caaacatgat atagttttga tctcctgata attcagaagc
    16921 gaatggttcg cctgcaaata aaacagtgat gatatatagc gggaccatca cgaccatgac
    16981 agacaaataa gtatcttgca gaaattcgaa atgtgtcggc aacttgatat cttcgatcga
    17041 ctttttattt tcgcctttct caccaaaaag cttagcaaca ccggcttcaa ataagtaccc
    17101 gatcgtacaa aaatgaccta aagcaatatc atttgatcca gtgattttac gaatgatcgg
    17161 ttgcgctact gccggcatag caatggcgaa acaagctccg ataaaaccac cgaccaagat
    17221 caatacgatc cctcgcagac cggcaaaata gccaaagact gtcgtcatcg ttgccatcca
    17281 taaaattgct tgaccagtta aaaaaatgta cttccatttt gtgaaacgag ccaaaataat
    17341 attgaataca aaaatggcta agaacgtcag tgcaatatct ctacctaaac ccaaatcatt
    17401 cattgcttga ccattgatag attcaataga agggatgatc ccttgcatat gaaatccttc
    17461 agtaaagatt ttcccgaaat aagttaaact tccaacgatg atgctcgaac cggcactcaa
    17521 cacttgaaat cctaggagtg tctttaacgt gccagagatg acttgtcctg ctgatttctt
    17581 ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat
    17641 attttcgatg ataaaattga taacgcccat gtt
//
LOCUS       DACCKU010000061        17240 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.064, whole genome shotgun sequence.
ACCESSION   DACCKU010000061 DACCKU010000000
VERSION     DACCKU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.064"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(97..1116)
                     /locus_tag="IVN13_001921"
     CDS             complement(97..1116)
                     /locus_tag="IVN13_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7012392.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="IVN13_001922"
     CDS             complement(1138..2145)
                     /locus_tag="IVN13_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP7012393.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IVN13_001923"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IVN13_001923"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP7012394.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="IVN13_001924"
     CDS             complement(2796..5090)
                     /locus_tag="IVN13_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP7012395.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="IVN13_001925"
     CDS             5350..7515
                     /locus_tag="IVN13_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7012396.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="IVN13_001926"
     CDS             7819..8652
                     /locus_tag="IVN13_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP7012397.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="IVN13_001927"
     CDS             complement(8754..10454)
                     /locus_tag="IVN13_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012398.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="IVN13_001928"
     CDS             10961..12121
                     /locus_tag="IVN13_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7012399.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="IVN13_001929"
     CDS             12137..12580
                     /locus_tag="IVN13_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012400.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="IVN13_001930"
     CDS             complement(13063..13593)
                     /locus_tag="IVN13_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP7012401.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="IVN13_001931"
     CDS             13698..14249
                     /locus_tag="IVN13_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7012402.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="IVN13_001932"
     CDS             complement(14402..14875)
                     /locus_tag="IVN13_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7012403.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="IVN13_001933"
     CDS             15013..16485
                     /locus_tag="IVN13_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7012404.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="IVN13_001934"
     CDS             16482..17192
                     /locus_tag="IVN13_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP7012405.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5359 a         3099 c         3544 g         5238 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggagaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gagttttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataagt actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ttcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagcca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacacccacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa
    15361 gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta ggaggttgtg
    17221 aaaaagctgc tctgcatcaa
//
LOCUS       DACCKU010000062        17184 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.065, whole genome shotgun sequence.
ACCESSION   DACCKU010000062 DACCKU010000000
VERSION     DACCKU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.065"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(543..2048)
                     /locus_tag="IVN13_001935"
     CDS             complement(543..2048)
                     /locus_tag="IVN13_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7012406.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLFENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="IVN13_001936"
     CDS             complement(2045..2731)
                     /locus_tag="IVN13_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7012407.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVN13_001937"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="IVN13_001937"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP7012408.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVN13_001938"
     CDS             complement(4515..4694)
                     /gene="rpmF"
                     /locus_tag="IVN13_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP7012409.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(4752..5321)
                     /locus_tag="IVN13_001939"
     CDS             complement(4752..5321)
                     /locus_tag="IVN13_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP7012410.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            5507..6409
                     /locus_tag="IVN13_001940"
     CDS             5507..6409
                     /locus_tag="IVN13_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP7012411.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVN13_001941"
     CDS             complement(6444..8228)
                     /gene="pyk"
                     /locus_tag="IVN13_001941"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP7012412.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVN13_001942"
     CDS             complement(8302..9264)
                     /gene="pfkA"
                     /locus_tag="IVN13_001942"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP7012413.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVN13_001943"
     CDS             complement(9432..12746)
                     /gene="dnaE"
                     /locus_tag="IVN13_001943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP7012414.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            12977..13162
                     /locus_tag="IVN13_001944"
     CDS             12977..13162
                     /locus_tag="IVN13_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP7012415.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(13224..14420)
                     /locus_tag="IVN13_001945"
     CDS             complement(13224..14420)
                     /locus_tag="IVN13_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP7012416.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(14590..16026)
                     /locus_tag="IVN13_001946"
     CDS             complement(14590..16026)
                     /locus_tag="IVN13_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP7012417.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            16319..17128
                     /locus_tag="IVN13_001947"
     CDS             16319..17128
                     /locus_tag="IVN13_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7012418.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5043 a         3594 c         2983 g         5564 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa acagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat attttttatt
     4441 taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt tttacatttt
     4501 ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata gtaaccacaa
     4561 tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc gtttaaccct
     4621 ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga tgttcttcta
     4681 gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat tagacacaat
     4741 atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag ttcagatagt
     4801 tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg ctggtattct
     4861 tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc ttcctcttct
     4921 tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga ctctgctaaa
     4981 ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc atctctactt
     5041 tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg cagttcaaca
     5101 ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta atgaacgata
     5161 tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac atcaagtatt
     5221 aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa agttaacggt
     5281 gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca ctcctaagca
     5341 acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt gacaccttgc
     5401 agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat catcctcaga
     5461 atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga acacgaaaga
     5521 tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg ctaattattt
     5581 ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag agtttgacac
     5641 cgtacttatt acaagagatc gatcgcatca cgaattgatc gtcacgcctt ctggaaagca
     5701 gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag ctgaaatcac
     5761 tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca atgaatactt
     5821 tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg agattaccga
     5881 tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt tagctgtatt
     5941 aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt tggacaaacg
     6001 tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg tctctttcaa
     6061 agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc ccgcagcatt
     6121 cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa gcaatgactt
     6181 gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg cagagattgc
     6241 tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc agcctcgctt
     6301 cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag aacaagtagt
     6361 ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa agaagagctg
     6421 tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta aacgatgccg
     6481 cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc gcctttagca
     6541 ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga agtcaatccg
     6601 ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc tttgtcagtt
     6661 gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc agcagatgaa
     6721 gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc taataattta
     6781 gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac tggtacacca
     6841 gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc ttttttagct
     6901 gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg gtaaacgccc
     6961 cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa gatatctgca
     7021 tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc agcaacgatt
     7081 gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt ttcagttaca
     7141 tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc attttcagct
     7201 tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc gtttgcagat
     7261 tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac gtcagaagct
     7321 tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt agcagtgata
     7381 actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat tggtactagt
     7441 tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc agaaactttg
     7501 atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa gatttgaaca
     7561 tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg tgctttacgt
     7621 acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat atcgtcagca
     7681 tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac ccctttacgt
     7741 gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc ttcgtctttt
     7801 tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc aacgtgaaca
     7861 tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac atgttcagga
     7921 tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc acgaccaaaa
     7981 tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt atctaaaaga
     8041 ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat acgtgataat
     8101 tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc agattcaatc
     8161 aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac gattttggtt
     8221 tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta cacagcttaa
     8281 atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat atagtgataa
     8341 gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa cgatttcgtt
     8401 gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga cagcgtaagc
     8461 accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac ggacaacgtg
     8521 tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct cagcgaattc
     8581 atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc cgcgatcgcg
     8641 gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg ggatgatgat
     8701 ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat tgcgtcccat
     8761 tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac ggtcaattga
     8821 ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc caggaatatc
     8881 attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca aagccattgc
     8941 gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga attttttcaa
     9001 ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac gtgcagaata
     9061 caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa gacgacgaat
     9121 atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca gtccttcgta
     9181 aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt ctccgccact
     9241 ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac tttatcctaa
     9301 gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct aacattattt
     9361 ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca agtagtattt
     9421 tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat aagttgccgc
     9481 tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt tcgttgcgtt
     9541 ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat cacttgtaat
     9601 tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca agtttgtgaa
     9661 ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag ttgcagttcc
     9721 tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc aacggcaaac
     9781 aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc tccactactg
     9841 tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc tctaacttcc
     9901 ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat cagctgaact
     9961 tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa ataaatacct
    10021 aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac ttcttcttct
    10081 tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat attttggatt
    10141 tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc aaaaactcct
    10201 actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat ccgtatgagg
    10261 aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat atcttctaca
    10321 aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc cttattgacc
    10381 aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag tttattttta
    10441 cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga attcatcagg
    10501 ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa tcccacgaat
    10561 gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc gatatattca
    10621 tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga tacaaaatgc
    10681 gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg cagaatatct
    10741 gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg ttcctgatag
    10801 acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg gtagcgaatg
    10861 gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg gcctggtcga
    10921 tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct tcgtaaaacg
    10981 ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg ttgaaacaag
    11041 agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata cccttctact
    11101 cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc taaaaagtcc
    11161 attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag aaaagcttca
    11221 tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat aactacacct
    11281 gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt tttgaataaa
    11341 agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt tttataagct
    11401 tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt tgcttcactt
    11461 tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc cattgtgcca
    11521 aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata attcaatact
    11581 tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa gcgttcgggg
    11641 tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt gatttctaac
    11701 acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac gattttgttt
    11761 ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa tcccatttga
    11821 tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata gtcagtgatt
    11881 cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg ttttccatta
    11941 ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga aatcgattca
    12001 gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa tcgtttgatc
    12061 atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc agataaattt
    12121 tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc aaaaagttct
    12181 tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc ttttatcaca
    12241 tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat ataaaaattt
    12301 ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc ctctttattg
    12361 aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc ggtttctgtc
    12421 atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt catcagctgc
    12481 tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc ttcataagta
    12541 taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat acgatagaat
    12601 tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta gccttgtttt
    12661 tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga ataagaagta
    12721 attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt taaaattcac
    12781 tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg aagttgatta
    12841 ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa gaactcaaat
    12901 tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa attaagatag
    12961 aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg ttctaggtca
    13021 agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta ctttgacaac
    13081 aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg cgccaagaaa
    13141 agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc aagctctttt
    13201 ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg aaagttgaat
    13261 aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac agcactgtaa
    13321 ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc aaagttgcta
    13381 aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat cttactgatc
    13441 agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa gcgaagtata
    13501 cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa tgataaaatg
    13561 agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc tagtttcggt
    13621 gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac aaaaaggata
    13681 ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct gatcgtagtg
    13741 attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga gagatgatcc
    13801 atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg atggaaatct
    13861 tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc tgtgatcaag
    13921 aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg tccgatcaga
    13981 tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt ttctctaggt
    14041 gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat cccattccaa
    14101 aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc cattgttaat
    14161 gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc agctagattt
    14221 ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc tagtcccgaa
    14281 aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg tatgaatggc
    14341 ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat cactaaattt
    14401 tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat acctattttc
    14461 gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag gtcattttat
    14521 atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt tccaatccct
    14581 tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa tatcaacgat
    14641 ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa ttgtatagaa
    14701 aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa agatcgatgc
    14761 aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa aagaagccat
    14821 catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta caatggcgat
    14881 cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg aagtcaaacc
    14941 agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg tattcgatgt
    15001 accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt tatccatttt
    15061 tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga aaatacctgt
    15121 gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata aactgaatgc
    15181 gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag attgcatgcc
    15241 ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat tacctaacga
    15301 attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac caaggactgt
    15361 ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta aaatggctgt
    15421 cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg ctaacgcagg
    15481 gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga cagagctcga
    15541 aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc cgacatacgc
    15601 aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa tcgcccgaat
    15661 aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc aaataaacac
    15721 cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa aagtgaaaaa
    15781 tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca atcccattac
    15841 cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga agggcatacc
    15901 tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa agaatgtcgt
    15961 tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac cgaaaaactt
    16021 ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa taataaaaaa
    16081 taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt tcattcgctt
    16141 ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt ttagcttatg
    16201 tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat tgctcttgtt
    16261 aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag gacccttatt
    16321 gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag attcgatgat
    16381 tacatctaaa acaaaagaga ctctgaaaaa agcaatcaat gccggtcatc atgtcagtat
    16441 tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag aattaacaac
    16501 accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact gggatggtga
    16561 aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa aaaagcagct
    16621 aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca gttttgattt
    16681 ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc tttctcctaa
    16741 aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt ttgcaggagc
    16801 tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga catggggtgg
    16861 acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag gcgtgcaaga
    16921 gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg acgagcataa
    16981 tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg gtactgacca
    17041 actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg gtttagctgt
    17101 ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa aagaggttgt
    17161 gacaattttt gtcacaacct tttt
//
LOCUS       DACCKU010000063        17004 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.066, whole genome shotgun sequence.
ACCESSION   DACCKU010000063 DACCKU010000000
VERSION     DACCKU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 17004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17004
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.066"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..124)
                     /locus_tag="IVN13_001948"
     CDS             complement(<1..124)
                     /locus_tag="IVN13_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP7012419.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVF"
     gene            complement(228..518)
                     /locus_tag="IVN13_001949"
     CDS             complement(228..518)
                     /locus_tag="IVN13_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003474616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7012420.1"
                     /translation="MVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYELDPSPLI
                     NRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQF"
     gene            complement(540..737)
                     /locus_tag="IVN13_001950"
     CDS             complement(540..737)
                     /locus_tag="IVN13_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP7012421.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1131..2858)
                     /locus_tag="IVN13_001951"
     CDS             complement(1131..2858)
                     /locus_tag="IVN13_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7012422.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2851..4044)
                     /locus_tag="IVN13_001952"
     CDS             complement(2851..4044)
                     /locus_tag="IVN13_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP7012423.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4231..4875)
                     /locus_tag="IVN13_001953"
     CDS             complement(4231..4875)
                     /locus_tag="IVN13_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7012424.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4969..5103)
                     /locus_tag="IVN13_001954"
     CDS             complement(4969..5103)
                     /locus_tag="IVN13_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP7012425.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5105..7237)
                     /gene="feoB"
                     /locus_tag="IVN13_001955"
     CDS             complement(5105..7237)
                     /gene="feoB"
                     /locus_tag="IVN13_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP7012426.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7234..7707)
                     /locus_tag="IVN13_001956"
     CDS             complement(7234..7707)
                     /locus_tag="IVN13_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP7012427.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8108..8332
                     /gene="nrdH"
                     /locus_tag="IVN13_001957"
     CDS             8108..8332
                     /gene="nrdH"
                     /locus_tag="IVN13_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP7012428.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8491..10650
                     /gene="nrdE"
                     /locus_tag="IVN13_001958"
     CDS             8491..10650
                     /gene="nrdE"
                     /locus_tag="IVN13_001958"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP7012429.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10700..11665
                     /gene="nrdF"
                     /locus_tag="IVN13_001959"
     CDS             10700..11665
                     /gene="nrdF"
                     /locus_tag="IVN13_001959"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP7012430.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11754..12893)
                     /locus_tag="IVN13_001960"
     CDS             complement(11754..12893)
                     /locus_tag="IVN13_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7012431.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13202..13915)
                     /locus_tag="IVN13_001961"
     CDS             complement(13202..13915)
                     /locus_tag="IVN13_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP7012432.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14169..14870)
                     /gene="nagB"
                     /locus_tag="IVN13_001962"
     CDS             complement(14169..14870)
                     /gene="nagB"
                     /locus_tag="IVN13_001962"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP7012433.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15104..16636
                     /locus_tag="IVN13_001963"
     CDS             15104..16636
                     /locus_tag="IVN13_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7012434.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16827..>17004)
                     /locus_tag="IVN13_001964"
     CDS             complement(16827..>17004)
                     /locus_tag="IVN13_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7012435.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2909 g         5322 t
ORIGIN      
        1 aaaaaacaca ttccactaaa tttgatgccg gttttccttg aggttttgtt ccagcacctc
       61 ctttaatttt tttaagctct tgatctgaac attgtttagc atttagtcta tttttttctt
      121 ccattacgat tccccctaca aactataaat acttttatgt cactgattca atatacctat
      181 ttttctaatc aaaaaatttt gagacatatt taatattata agtatcatta aaattgagat
      241 ttatctccat aatctgctcg atataaacca tttattccag cagttcttcc aatttcactc
      301 aaatttctta ttaattcctc ttgatattca gtgaatctta ttttattagt ataagcagta
      361 aaatatagat aattcaccaa tctatttatt aaaggactgg gatctaattc atatttttgt
      421 ttttctaatt ttaaatatac cttttgtaaa acctcaagaa tatcagaata tttaggatct
      481 ttacttatag atatatcatt atataattca tgaacaattt gcttagcatt tttttcattt
      541 tagcacttcc ctggaattgc tccacctaaa aaaccaccta tagacattcc tgcaatacaa
      601 gtagttgcct tggcccaatc gaccgtacat ttatttttag tgcaatacac tccatttcca
      661 taatattttc cactatgagt ggtaccccca tagataagtt gtgtctcttt aatagacaaa
      721 atttttaaat gtttcataat ctaatcaccc ctaaattaat gtttttagac tattattcta
      781 acattcatta aatttcaaaa aacaattcct tcttgaaacg tagcaaaata attcctgaat
      841 aagaatttaa tcacttttaa gtatataaac tatgtatatt ctttaatatt ttttgagaat
      901 gcacttcgta cataataaac tttaatcaca ttagtctttc atacctacga tcgatctctt
      961 tgactcgcac ccataaattc ataatagttt gtcggcgtct ttgaacgttt cgaagatgag
     1021 ctacgcattc tcttttttta tagacaaaaa agatagagac gaacatggag cttccaaatt
     1081 tggatatttg atccaatttt ggaagctcca tttatttcgt gtctgtcttt ttattttttc
     1141 cagctttcga ctaattcttg gttctctttg atccagtctt ttgctgcttg ctgtggatct
     1201 tttccattgt tgatttccaa cataactttt tccatatctt tttctgacca atggaaattg
     1261 tctaatactt tgtaagcttc tggctggtct tctttcaatc cttttcgaac catcgtatgg
     1321 atctgctctt cgcttcccat tccgttttca ggatcttcta agtatttcag atcatatttg
     1381 gcaaacatcc aatgaggtgt ccatcctgta actacaattg gttcgtgttt tttgatcgct
     1441 tgacctagtg caacggtcat tgctcctgaa gaagatgtct ctactttcca gtctttcaag
     1501 ttgtcatatt tttggatcgt gttttcagca gccgtaacta caccagctcc tggctcgatt
     1561 cctatgattt tcttacctgc ctgatcggtt aactcatcga tcgaatttac atccatgtag
     1621 gctggcactg ctaaaccaac ttttgctcct gtcaaattgg cacctaggtc ttcgacttga
     1681 tctttatatt gtgcatattg tgaaccatga gttttaggta gccaagctga aaccatggca
     1741 tctgactcgc catttgaaac agatttccac atgattgaat tatctaatgg cgtcatcgtt
     1801 acatcatagc ccatttgttt caatacttca ccgactacat ttgttgaagc aacttctgta
     1861 tcccattcaa cataagataa gttgatacgt tttgtttctt tggcagatga aaaactccct
     1921 ataccaatcg cacctaagat cactactact acagcaccaa tgatcagtcc ttgacgtttt
     1981 ttacttttct gtgttgttgc tcctgcagct tttttattgt ttggctggtt caaatgctgt
     2041 gtaaaacggt caatgatgat tgctaagata actaatgcaa ccccgttgac aaatccattc
     2101 ccgacttgtg ctctttgcaa tgctgataat acgccgcgtc ctaagccggg tgcaccgatc
     2161 atagaggcaa taacgaccat tgaaagtgac agcattgttg tttgattgat ccctgccatg
     2221 atcgtacttt tagctaaagg caattctagt ttgaatagtt tttgacgact tgtgctcccg
     2281 aatgaatcag aagcttctac taattcttta ggaacttgtc gaatccctaa attcgtaaaa
     2341 cgaacggttg gtggcagagc aaagatgact gaggcaaata cccctggaac catcccgata
     2401 ccgaaaaatg caactgctgg aatcaaataa acgaatcctg gcattgtctg cataaagtca
     2461 aggatcggtt tgataatact ttgggcttta ctgctttttg ccatcaagat ccctaatggt
     2521 accccaatga tgatcgagat cacactagat aaaagaacaa gtgtgacagt attcatcaaa
     2581 tcattccata gaccttggtt ataaataaac catagaccga ttaatgtgaa caacgtaagt
     2641 cctggacgtt tcttagaaat aaagaaagct gctaccgtca ataacaagat aaacaaagga
     2701 gctggaatca ctaacagtaa atttgtaatc cccgacatca ctgcctgtcc aatcgtttgt
     2761 aaaaatgaaa ataggccaga aaaagttgtc gtaaaccatt ctgtgatatt ttctacccag
     2821 tttgccactg gtaattgata gttcaataga ttattcatgt tcactcacct cgcttgtttc
     2881 tgctaatgct tcaatcacac tacctcggat gacgacacct tttaattttc catcctctac
     2941 aactgcaagt ggagtcggtg agtcataaat aatattgaaa atatcactta ccaccatgtc
     3001 tttcccaatc accgtcacat tttcatctag gaagtcgatc aatggacgtt tttgcttcct
     3061 agcttctaac gcttgatctg cagtgatgat ccctttcagg tgtctctttc tgtcaacagc
     3121 catcagcatg ctgacttctt cattacgcat ccttgtcaat gcaacgtttg gtccatcaga
     3181 ttcgatattc gtcgtcaatg caggtaccat gatgttttga gccgtaagca ctttggaacg
     3241 atcgacttct tccacaaatt cacgtacata atcatttgct ggattggtca atatttcttc
     3301 acctgttccg atctgcatga tttcgccgtc tttcatcaag gcgattctgt ccccaatccg
     3361 taaagcttca ttcaaatcat gggtaatgaa aataatcgtt ttttgaacat tggcttgcag
     3421 gtccagtaat tcgtcttgca tttctcgccg gatcagcgga tcaagagccg agaacgcttc
     3481 gtccatcagc aagatctcag gatcatttgc taaagctctg gctaggccaa cccgttgttg
     3541 catcccacca gataactgac taggatattg gtctttaaat gaaagcaaac tagaattttc
     3601 taatgctttt tctgcttttt cttgtcgttc ttctttaggt acaccacgga cttctaaccc
     3661 gtattctgta ttttccaaga tcgttcgatg tggaaacaga ccgaagtttt ggaaaaccat
     3721 attgatttta tgacgacgga cttctcgcaa ttcttctttg cttaacttcg atacatcttc
     3781 tccatcaata tagatactgc ccgaagttgg ctcaatcaga cgattcaata gacggatcaa
     3841 tgtggatttc ccactcccgg aaagccccat gatcacgaag atttctcctt cttttacgtc
     3901 aaaattcacg tcatacacac caaccgttgc accggttttc tttaaaatct ccgttttcgg
     3961 ctggtgatca ttcatcatat ctaaagcggc ttgtgtcttt ttaccaaata tctttgttaa
     4021 atgttttact tctactttta ccaaacaaat tctccttttt tatgtaaatt catttttctt
     4081 tctgaaaaaa gtgaccacac tatgttaaca tcatgtagtc actttgtcaa aaatgaaaga
     4141 aatttgcttg tccaaactga aaaaaacttg gctaaatctt tgtaaataaa aggtttagcc
     4201 aagttaatat ttttttaata tttcacacaa tcattctgca agctgatgct ccgggtaaaa
     4261 aaagtttgtc atacgctgta gtgtatattc atttccaata aaatgaacga tgtcccctgc
     4321 cgtaaattta gcataggggc caggtgaaac aagcagttct tcattatgag aaattgcgat
     4381 aactgtggcg ccagtcgcct gccagatatt caactcgcta attgaacggt ccagatgcat
     4441 tgctttttct gataaagata attcaaacgg aagtaaagga ttcatcttat gtattcgctt
     4501 cgtttgattg accagctgat ccaataattg agaaaaatga ttcagctcat cttgttgctt
     4561 ctccacactt gccatgattt ctgcttttgt atcttggatg gattgtacat cttgatattg
     4621 caataggaaa tcttttgctt tttccttcga agcaacaaat gccccacttc catgccgtac
     4681 ttccatgatt ccaagatcta ccagcacatt gatagccttg cgggtagtct caggagaagc
     4741 attgtacgta ctggcaagtg ttgagcgggc atggatcttc tcacccactt tatatcggtt
     4801 ttcaacgata cgttcggcta aatcaacagc aatctgctgg tagcgcgggc ggacgatttt
     4861 tttctttcca gacattttct ttcctctttc tttttttact ttccctcatc ttactatgct
     4921 ctagcaaaat aagcaaaaaa ggggagaata gcctcttgca acgactgatc atcgcttgat
     4981 ctgttccttt tttactgggc aagctgtttg acagtcatca caattcttac cggattttaa
     5041 acgagtatag acaattgcgc ctgcacttcc aaagattaac acacttaata agaatgtcgc
     5101 catcctattc ctctccttct aatgacaata tagtataaaa ctggttcttg gattttggct
     5161 gacggaacaa aacataaacg agtcctatca tcagtaaaat agcaagatac gtccctaacg
     5221 ctacctgtcc accctccaat aagtgtccca actgataaat cacaaaacta actaaataag
     5281 ctaaaccgca ttgatagcct atggcgatcc aagtccattt cttggcattc atttcccgat
     5341 ggatagcccc aattgccgca aaacaaggcg cacaaagtaa attgaaggct aacaatgaat
     5401 aagctgctgc gggagtatat gtgtgttgta gcacttgcca gatttctctg ccattttcgg
     5461 atacttccgc atgaccaaac aaaataccaa atgtgccaat aatattttct ttagcgacca
     5521 atcctgtaat cgctgcgact gttccttgcc agtttcccca accaagcggc tgaaaaacaa
     5581 ctgccaggat tcgcccgaaa aaggctaaga tactttggtc ttcagaaaca ggttgagcat
     5641 aaaaattata agaagagctg aaccagatca cgatactaga aacaaagata atcgttcctg
     5701 cttttttaac aaaagacttg ctgcggtcat aggtttgtct taggaccgct gaccattgag
     5761 gaaaatgata tgcgggcaat tccatgataa aaggagctgt atctccagca aacaaacgtg
     5821 tttttttcaa gctgattcca gagaaaataa ttgcagtcat accaagaaaa taggcagaag
     5881 gagctaccca agattgatga ggaaaaaagg cccctgctac taaggcaata atcggcaact
     5941 ttgcagaaca aggcatgaag gttgtgacca tgatcgtcaa acgacgatct tgctcgtttt
     6001 caatcgtgcg actggccatg acgcctggca ctccacatcc tgtagcaatc agcataggaa
     6061 tgaaggattt tcctgaaaga ccgaatttcc tgaaaagtcg atccatgaca aaagcgatcc
     6121 tcgccatata tccgcagtct tccagaaagc tcaagcataa aaacaatata atcaattgcg
     6181 gtagaaatcc taacactgct cccacgcctg cgatgatgcc gttcaagatc agctcctgca
     6241 tccatcccgc tacttgccat tcagctaaca atctgccaac atgttctggt aaccatgtac
     6301 caaacaatgt gtcattgatc caatctgtcc ccattgttcc tactgtttga atagccagat
     6361 aatagatcag ccacatcaca aatgcgaaga tcggcaaggc taaccaacga ttcgttgcaa
     6421 tctgatcgat tttgtcactc atcgataatt ggaaagaatc atttgacaca acacagagtg
     6481 tacatagttg ggtaataaat tcataacgtt cattgaccag aatcgtctca ctatcgtcgt
     6541 ccaatagttt ctctgttaac tggatcagtt gctcgatctc tttttgctga gaaactgcta
     6601 tctcgtaatc ctctcttacc ttctgatctc gttcaaaata tttcattgca taccagcgtt
     6661 tcttgccgct ttgttctcct agtacatctt caatttcatg taaagctgct tctaaacgtg
     6721 gatcatatgt acagggaaca ggaatagaag gccgcttttt tgcagactcc tgaatctttt
     6781 cgattagttt ttcgattcct tgctttttca atacgctgat tggtaaaact ggtacaccaa
     6841 gaccatagga aagcttttct agattgattt tttttccttc ttttttgatg acatccatca
     6901 tattcagacc aatgatcata ggaatgtcta aatccaataa ctgcgtagtt aaataaagat
     6961 ttcgttctaa attggtagca tcaataatgt tgatgatcag ttcaggctgt tcctgaacca
     7021 aataatctct tgcaatcact tcttctggtg tataaggaga caaagaatag atgccgggta
     7081 ggtcctgaat atgaatgttc ggattttttt tcaacgatcc ttcttttttt tctactgtta
     7141 cgcccggcca attccctaca tattgattcg aaccagtcaa gagattgaac aagctagttt
     7201 tcccgctgtt tggatttccg actaacgcga tattcattct ttttcctccc tttgtatcaa
     7261 gattagttcg gcctctgact ttcgtaacgt cagttcatac cctcgcactg tcagttcgat
     7321 tggatctccc agaggggcta gttttcggat aactattttt acatttcgcg tcaatcccat
     7381 atccattaac cgtcgcttca ctgcgccttg tccgtggatt gctacgacga tcccagtctc
     7441 tcctgctttt aattgatcaa gagaagtcca gctttcagag gaagcagtct ttttattgac
     7501 aaagatttga cctagtattt cccgacttac tgccactcga tgacttttta ccagtaaaat
     7561 ggcattgttg cctgataaat tgacgacgac gattttactt ccggcaacga tcccgatatt
     7621 ttgcaaatgg cgttttgttt tttcttctgt ttcgattcgt tcgacttgat aaacgaaatt
     7681 ctgctttgcc tgcgctaagg tctgcatatt gattcctctc ttgtgaaata aatcatgtat
     7741 taaaatcatt cattatatgt tctaattttc ttacgtgtta cgcaaataaa tgagaatcat
     7801 tctcacatag aatactctcg gtaattgaga atgtcaatta tacttgtgat attatgagcg
     7861 aatctttagt attgatagat aaaaatgaga aaaaaatatt tcttcatagt tttccacaat
     7921 tttttctcta ttaaatttgc tattttccca cacgatactt gttttcagct cttttttcaa
     7981 aaacacaaaa cttagtattc cacagtttcc acaacacaat atgtagtgtt tgttgtttga
     8041 cttttttact aagagtttta tactatctat tatagataat aagaatgtgt tagaggaggc
     8101 actcaccatg aacgtaaaaa tcttttctaa aaataattgt atgcaatgta agatggtcaa
     8161 acggttttta acagaaaatc aaattgcttt tgaagaagtc aatatcgatg aacagcctga
     8221 agcagtagac tggttgaaag cacaaggatt ccaaagtgtt cctgtcatta cttctgatgc
     8281 agaaacagtt atcggattcc gtcctgatca gttgcgtaaa cttgccagct aacatgacta
     8341 aaagaatcaa aacgtgatac atggatttga agggcttgag acagcaccag tttttgtcgc
     8401 aaacttccaa atcctttttt aaagcttaaa aacaatgcaa tgatttgcaa tcaagcatca
     8461 tcaaatacga aatgaaggtt gggcgtacgt atgagtctta aaaatttaca agatgtcagt
     8521 tatttcaagc tgaataatga aatcaatcgg ccagtcaatg gtcaaatccc tttgaataag
     8581 gataaagaag ctttagttgc cttttttgag gaaaatgtca aaccaaatac gatgactttt
     8641 ccatctatta tggaaaaaat caattacttg atcgatcaag aatatataga agcagaattc
     8701 attcaattgt attcaccatc atttattgaa aagctatatc aatttttgga cgagcaacat
     8761 ttttctttca aatcttttat ggctgcttac aaattttatt ctcaatatgc gttgaaaacc
     8821 aacgacggtt ccgcttatct ggaaacttat gaagatcgag tagccttcaa tgcgctttac
     8881 tttgcgaacg gggatgaaga attagcactt gctcttgcag acgaaatgat ccatcaacga
     8941 tatcagccag ctacaccttc tttcttgaat gctggacgta aacgtcgggg cgaattggtg
     9001 tcttgtttct tgactcaagt cactgacgat atgaactcta tcgggcgctc aatcaactct
     9061 gctttgcaat tgtcccgtat tggtggcggc gtagggatta ctttatcaaa tcttcgtgaa
     9121 gccggcgctc ctatcaaagg atatgagggt gctgcaagtg gtgtcgttcc agtcatgaaa
     9181 ttattcgaag atagtttcag ctattctaat caattaggtc agcgtcaagg agctggcgtg
     9241 gtttatttga acgttttcca tccagacatt attgctttcc tttcagcaaa aaaagaaaat
     9301 gcagatgaaa agattcgcgt aaaaactctt tctcttggcg tgatcgtacc tgataaattt
     9361 tatgaattga cacgtaaaaa cgaagatatg tatttattta gtccatatag tgtcgaaaaa
     9421 gaatatggtg tcccattctc ttatgtaaat attacagctg agtatgatca tctagttgca
     9481 aatccaaaca ttcgcaaaca aaaaatcaaa gcgcgtgatc ttgaaaatga aatctcaaaa
     9541 ttacaacaag aatctggcta tccttacgta atcaatattg atacagcgaa ccgcgagaat
     9601 ccaatcgatg gcaagatcat catgagtaat ttatgttcag agattttaca agttcaaacg
     9661 ccttctgtga ttaacggcaa acaggaatat gaagtattag gaacagatat ctcatgtaac
     9721 ttaggctcga caaatatcgt gaatttgatg caaagcccta actttggtca atctgtgcgt
     9781 acgatgactc gtgcattgac atttattaca gatgcatctg atatcgatgt cgtaccaccg
     9841 atccaaaacg gaaataagct gaatcatacg attggattag gtgccatggg cttacacact
     9901 tatcttgcaa aagaacaaat ggaatatggc tcagaggatt cattagactt tacaaatatt
     9961 tacttcatgc tgttgaatta ttggacactt gttgaaagca acaatatcgc tcgtgaaaga
    10021 aaacaagttt tccataattt tgaaaaatca gcttatgctg acggcactta tttcgaaaaa
    10081 tacgtaactg gagaattcca gccaaaatca gaaaaagtca aagcattgtt tgaaggcatt
    10141 tttatcccaa ctgctgaaga ttggaatgct ttgaaacaag cagtaatgaa agatggactg
    10201 tatcatcaaa atcgcttggc agtagcacca aatggctcaa tttcatatat caacgatacg
    10261 agtgcaagta ttcatcctat cactcgcatg atcgaagaac gccaagagaa gaaaatcggt
    10321 aaaatttatt acccagcgcc ttatctatcc aatgagacga ttccgtatta cacttctgct
    10381 tatgatatgg acatgcgtaa agtaatcgat gtttatgcaa cggcacaaca acacgtcgat
    10441 caaggcatga gtttgacttt attcatgcgt tctgaaatcc cagaaggcat ttatgaatgg
    10501 aagacaacaa cgaagcaaac gacacgtgat ttgaatattt taagacatta cgctttccac
    10561 aaaggaatca aatccattta ttacgtacgt acctttactg atgatgcaga agaaatcgga
    10621 agcaatcaat gtgaaagctg cgtgatctaa tatcatagat agattcgctt attcatattt
    10681 taattaaagg agcattctca tgtcagaaac atattatgaa gcaataaact ggaatgaaat
    10741 tgaagatatc attgataaat ccacttggga gaagctgaca gagcaatttt ggctagatac
    10801 acgtatccca ctttcaaatg acctagatga ttggcgtaca ttgtctcaat tggaaaaaga
    10861 tacagtagga catgtatttg gcggtttgac actgctagat actgtccaat ctgaaagtgg
    10921 catggatcag ttgcgtaaag atgtccgcac tccccatgaa gaagccgttt tgaacaatat
    10981 tcaattcatg gaatccgttc atgccaaaag ttattcttct atttttagta cactgaatac
    11041 gaaaaaagaa atcgaagaaa tttttgactg gacaaatacg aatccttatc tgcaaaaaaa
    11101 agctgagcgt atcaatgaaa tctacaaaca cggtacaccg ctagaaaaga aaattgccag
    11161 cgtcttttta gaaacattct tgttctattc aggtttttat actcctcttt attatttagg
    11221 aaacaacaaa ttggctaacg tagcagagat catcaaacta atcatccgag atgaatctgt
    11281 acatggaacc tacatcggtt acaaattcca attaggattc aacgagttac ctgaagcaga
    11341 acaagaaacg ttaaaagact ggatgtacaa tcttctttat gagctttacg aaaacgaaga
    11401 acgttataca gaagaattat acgatccaat cggctggaca gaagaagtca agacattcct
    11461 acgctataat gccaacaaag cattgatgaa cctagggatg gatccactct ttccagatac
    11521 tgccaatgat gtcaacccaa tcgtaatgaa cgggatttca acaggcacaa gcaaccatga
    11581 cttcttctca caagtcggaa acggctattt gttaggtcat gtagaagcga tgaaagacga
    11641 cgattatttg attggattgg aataaggatt tattttaatt caaaaatttt tcgaaaaata
    11701 aaaagagttt agcgtacaga tattttaaat gtacactgaa ctctttttta tgcttattca
    11761 cttgtttctt ccagcacttt tggactgtct aacttttccg aaacatgttc cattcttctc
    11821 aataaataga ttttccggat atttaatatt atcgtcttaa tcactgcata gacaggtatg
    11881 caaatcagca tgcctaatat tcctgcgaca cttcctgctc caatcaatac caagatgatc
    11941 gtcaaaggat ggatattcat tgatttccca aataacaacg gcttgatcaa atttccatct
    12001 aattgttgga tcactaaaat cgacaacgcc atatacaaag cttgaaatgg agagataaac
    12061 aaaccgacaa tgattgctgg taccgctcca ataaaagggc cgacatacgg gataatattc
    12121 gtgattccgc aaatgaccgc aagcaacaag ctataaggct gatcgaatat tctcattgcc
    12181 aaaaagctca ttacaccaat aatcaaagca tctaagatcg tactgctgat ataggcagat
    12241 aatgtttcgt ttaattcttt taccgtttgg cgtaactctt tacgaatcgc tttcggaaag
    12301 aagtgagaca acgcatcaat aaatttgtgc ccatccttga acataaatag caaaataaat
    12361 ggaacggtaa ataacaaaat aaaaaattgc atcataaatg aaatgatttt tgagacactt
    12421 gcagataaac tgactaatat gatattgaac aaattactaa gagataaatt tgctgatgct
    12481 aattgttctt ccaaattgaa atttttaaac tcttcccgct gcgccaattc atttaaccat
    12541 ttgcttgttt catctgcata cattggcaag ctctgggtca attgaattgt ctgttccatt
    12601 aactgaggca ctacattcat gacagttaaa acaactaaaa tgattacgac caaaaacgtc
    12661 aataaaaaac cgaacactct cgggatccga tgcttttcga gaaaaacaac gattggatcg
    12721 aacatataat agataaaacc agcaacaagc aatggtaaaa aaattgccga aagcattttc
    12781 aatattggac taaaaatatt cggcatttgt aaaataaagt acacgccgat gatcgtgagt
    12841 actagctcca ctgtccagaa aaataatttt gattgtttaa aacgtgattg catacatctc
    12901 ctccaaggtc ctttgatact tctattctat catttaacaa agagttacgc gctgagattt
    12961 tcatttgctt aaaaaaaaca aaaaaaataa acatatttta atgagtagac gagtacttaa
    13021 acaaaaacac ggtttggagt agtgacaaaa attatactgc agtccctttt tccaaataaa
    13081 cggtgttcaa aagcttgcat cttcgtaata agcccagcca aacgaaaaat ataaggaact
    13141 atttttattt ggctgggctt attacttgat gtaggacatc tattttacaa cctcagatca
    13201 tttattgcgc aatactgact aaatgcaact ttgtgatatt tttgtgtacg gacaactcct
    13261 cgatcacatc ggcataagtg agcccttttg gaagatcaat cgtatagata tttttgtaca
    13321 ctcggtagtc atcaacgaat tttacgtcaa aattcacatc ttcgattacg atatttttct
    13381 cttcaaaata atcggtaaga aaatctttgg tttcttttct atggataaat tggatttcta
    13441 actttttagt cgtaggtaca tggatcacgc gtttcactac tgtgagtgca atagcaatcc
    13501 cgataaaact gatgatcgca atggaatagt aacccatgcc gatggcgatc cctaatccag
    13561 caattgccca taaagaagcg gcagtcgtaa gtcccgtcac cgattgtttc gtgacgatga
    13621 tcgttcccgc gcctaagaaa ccgacaccac tcaccacttg agcaatcagc cttgcttcat
    13681 ctgaacgaat cgttccagca agctgtggat actctaaagc atctcttaat gcaccactag
    13741 cgatctcaac ttggatcaat gcaatgatac atgctcccat acaaactaaa atatgcgtac
    13801 gcatacctgc tggacgattt ttatattgtc gttcaaaacc gattgctccg ccaaatagca
    13861 tagcgatccc taaacgtaaa ataatctctg gaattgtcaa taccatatca ttcatctaaa
    13921 caccctccag tccaatatat catgtctgaa aggagcagac aaaaaaagtg caatttttac
    13981 ctcttacgaa aaaaggactg tgacaaaatt tttgtcatag tcctttttat gaataaacgg
    14041 tgttcaatca gctgaccctc ggtattactc aacaaacggc aaaatatggg gagctatttt
    14101 tcctttgtct tctctaatac tcaggtcaaa acgctgattt cacaacctct ttagcctact
    14161 attgaaaatt acaacttact tgctgcttct tcatcgatga tcacaacaac attgtcgtga
    14221 ttttgcaata cacttgctgg caaatgattc gttacaggtc cttcaatcat ccctttgatt
    14281 gcatcagctt tgctttcgcc aaatgccatc aagacgattt ctttactttt cataattgaa
    14341 ccgattccca ttgaaacagc ttttgttggt acatcttctg ctttatcgaa gaatcgtttg
    14401 tttgcattga ttgttgattc tgttaagtca actactgaag tcgttccgtc aaatgaagca
    14461 ccaggttcat tgaaaccgat atgtccattt tgcccgattc ctaagatttg gatatcgatt
    14521 ggatgtgcat cgatgatttg atcatacttt ttgcatgctg cttccagatc ttctgctttt
    14581 ccatctggta caaacgtttc tttgaatggt tttttatcaa ataattggac atccatgaaa
    14641 tgacggtagc tttgttcatc tgaaccgcct agtccaacgt actcatctaa gtttacagaa
    14701 gtcatttcac tgaaatccaa atcgcttgaa gtcatttctt tatataaagt aactggtgtg
    14761 cttcctgttg ccaatcctaa tacttttgca cctttgttca taccttcttt gatgatttca
    14821 aatgcttttt ttccgccttc ttcagcgttt tttacacgaa tgatttccat agtgattgct
    14881 cctttatatt ttggtatagc ccaattatag cattcttttt ttctttttac aataattagt
    14941 atagaccaaa ttgcattttt ttcattttct tgacaataaa taggaatccg tgttaggttt
    15001 cataagatgg ttcaatcaaa caatccatgt tgcatgaaaa ccaagcaagt gattcactgt
    15061 ggacgcttta tgtctgcagt ttttttatga aaaaggagtt attatgagta tactgacatt
    15121 aaacaacatt acccagcaat ttggcgacaa aatcctctat gaaggtgtcg atctgcaatt
    15181 aaatgctggc gaacatcttg ggctgattgg acaaaacgga gccggtaaaa gtacattgat
    15241 taaaatcatc actggtgaga tcttaccaga tgacggacag atccattggc aaaaaaatat
    15301 tcacaaaggt tacctcgatc aatatgtgga agtcgatgaa acattaacaa tcgaagaatt
    15361 tctaaaaact gcgtacgcaa aagaatacga aaaggaagaa aaaattgctt ttctttacca
    15421 aacatatagc gagaccttcg atgattcctt gctggaaaaa gctgggaaac tccaaacgga
    15481 attagaccaa ggagtgtttt atcaaatcga caccttagtt gctgagatga gcagcgggtt
    15541 ggggatcgat gtcttaggtt tagattcact gttgaaaaat ctaagtggtg gacaacgttc
    15601 aaaggtgatt ttagcaaaac tacttttaga aaacccagat gttctgatct tagatgaacc
    15661 aaccaatcac ttagatgatc agcatatcca atggctgacg cagtttcttc aggattttga
    15721 cggtgcgtat atcgtgattt cccatgacca agaatttctt gatcagatca ccacccatat
    15781 cgcagacatc gagtttggaa agattactaa gtatactggc catctaaaac aagcattgaa
    15841 acaaaaggaa caaaatcgcg aaagctattt gcgccaatat catgctcaac aaaaacaaat
    15901 cgaaaagacc gaagcttata ttcgaaaata caaagccggt tcccgatcaa cgatggcaaa
    15961 aagccgacaa aaacaattag ataaaatcga acggctgacg cctccagctt cagcaagtaa
    16021 agctgtcttt gattttccat tctcgcctat tgtcacgaca ttagcagtgg aaacaactga
    16081 tttagtcatc ggatatgatc gaccgctact tgcacccatc aatttgacca tccgctttgg
    16141 tgaaaatatt gccattcgag gcttcaacgg gattggaaaa tctactttat taaaaacact
    16201 gatcggtgag attgaaaaaa taagtggcga tttccacttc ccagataaca caaaaatcaa
    16261 ttacttttca caagatctgt actgggaaaa tccattagaa acaccactac aatatttaag
    16321 cagtcaattt ccaaaagcga ctatcaaaga attacgtcgc caactagcta aagcagggtt
    16381 agttaatcaa ttagcaagtg aacctctcac tacattaagt ggtggggaac aaactaaggt
    16441 gaaacttgca cagttgacaa tgaaccaagg taatttactg attctcgatg aaccgacgaa
    16501 ccatatcgat caagaaacga aagaaagctt acaagacggg atacagaaat ttccaggaac
    16561 agtaatcatc gtctctcatg aacaagaatt ttatcaagat cttgtgcaac gagtcattga
    16621 gattgaacca aaataaacgt ttgattgaat agttgaaaaa aaggagctgt gacaaaagtt
    16681 ttgtcacaac tccttcttct gaataaatgg tggccaatta gctgaccttc ggtattagct
    16741 caaaaaacgg caaaatatga ggagctattt ttatttggct attcttatta cttgatgcag
    16801 aacagctttt cacaacctct tttcgtctat tttttatttt cgagatttct ctttccttta
    16861 aaaacaactg tttgaaagaa gaacgcacat ccttgaaaaa caagcccgct aaccgttaca
    16921 cctaaccagc catactgctg ccacattgtc gtccctagca atgaaccagc cgcacctcca
    16981 ataaagtaca taaacataaa taca
//
LOCUS       DACCKU010000064        16847 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.067, whole genome shotgun sequence.
ACCESSION   DACCKU010000064 DACCKU010000000
VERSION     DACCKU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.067"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(143..676)
                     /locus_tag="IVN13_001965"
     CDS             complement(143..676)
                     /locus_tag="IVN13_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012436.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(973..3354)
                     /locus_tag="IVN13_001966"
     CDS             complement(973..3354)
                     /locus_tag="IVN13_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP7012437.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(3344..3991)
                     /gene="recU"
                     /locus_tag="IVN13_001967"
     CDS             complement(3344..3991)
                     /gene="recU"
                     /locus_tag="IVN13_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP7012438.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            4055..4594
                     /locus_tag="IVN13_001968"
     CDS             4055..4594
                     /locus_tag="IVN13_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP7012439.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            4684..5103
                     /gene="gpsB"
                     /locus_tag="IVN13_001969"
     CDS             4684..5103
                     /gene="gpsB"
                     /locus_tag="IVN13_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP7012440.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            5125..5492
                     /gene="rnpB"
                     /locus_tag="IVN13_001970"
     ncRNA           5125..5492
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IVN13_001970"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            5701..6867
                     /locus_tag="IVN13_001971"
     CDS             5701..6867
                     /locus_tag="IVN13_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP7012441.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6913..8409
                     /locus_tag="IVN13_001972"
     CDS             6913..8409
                     /locus_tag="IVN13_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP7012442.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            8490..9173
                     /locus_tag="IVN13_001973"
     CDS             8490..9173
                     /locus_tag="IVN13_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP7012443.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9818..11008
                     /locus_tag="IVN13_001974"
     CDS             9818..11008
                     /locus_tag="IVN13_001974"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP7012444.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            11110..12063
                     /locus_tag="IVN13_001975"
     CDS             11110..12063
                     /locus_tag="IVN13_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP7012445.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            12132..13481
                     /locus_tag="IVN13_001976"
     CDS             12132..13481
                     /locus_tag="IVN13_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7012446.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(13664..13987)
                     /locus_tag="IVN13_001977"
     CDS             complement(13664..13987)
                     /locus_tag="IVN13_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012447.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            14116..15279
                     /locus_tag="IVN13_001978"
     CDS             14116..15279
                     /locus_tag="IVN13_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012448.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            15349..16698
                     /locus_tag="IVN13_001979"
     CDS             15349..16698
                     /locus_tag="IVN13_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP7012449.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(16743..>16847)
                     /locus_tag="IVN13_001980"
     CDS             complement(16743..>16847)
                     /locus_tag="IVN13_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012450.1"
                     /translation="MTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
BASE COUNT         5443 a         2922 c         3520 g         4962 t
ORIGIN      
        1 agaaaccacg cctgtagacg tggtttcttt aattgcaaat aaatgtatga attatcttgt
       61 tattatggag aaaagctgtt tcgtttatct tttttgcatg aaaaagtaaa tgtacatact
      121 cagtagctag gatggtaact tattacaatt tattgcgata aaaataaaag ttgttatttt
      181 tattgggaaa atacccgatg tagaaaataa tgccttccaa tatacctata aaataagtcc
      241 agtattccaa aggattatat aaataagtga cgtatctatc taaaggtgta ataaaaatga
      301 taagtgataa taaaaaaata ccatttataa ctaaacataa actaatggat ttgggcggtt
      361 tcaggctcat cagaaaaata tagattctgg tgaacaacaa ccataaaaaa gtgagaatca
      421 ttgcatgaga gcttctttcc ataaattttc ctttaagagt agtgcttaaa agtacagaaa
      481 aaataataaa tgcaaggata ggagcaatta aagtaaattt atttaataat aaacaaaatt
      541 tttcagcttt tttattcttg gaataacgag gtattgtttt cgataaaaaa agatggtgca
      601 gacaaattat gataattaag catactatca gataactcaa aatgaagcct tctttcaaaa
      661 aattattcaa aaccatatat tgtatttttt atttgataat aacaatggga ttattaagaa
      721 gtggcactaa ataaaagact agtgtgtatt tagccgattt aattataaac aagtaaatgg
      781 ataaagtata tgtaatgata aaagaaaaat ggattttcta atttttaagc taaatgttag
      841 atcacacatg tatatgctgg atagtcttat aaaaaatcat gtaatacagc ttggtacatg
      901 atttttattt gtagctgttt tttacataga cacaaaaaag aaaccacgct tgtagacgtg
      961 gtttcttttt tactatctgt ttccagaagc tgaggtgctg ctggatggtg tatggtcatt
     1021 tgcagcatcc gctccgctag ttgctggctc actggaagaa gcaggaggag cagatgaaga
     1081 cggtgtcgag ctttcttcgg atgaagaaga aggaaccgtt gttgaagacg ctggctcgct
     1141 tgtttctgct gtcgtgcttt cttttgagga ctcagcagtt gattcgcttt gactagtaga
     1201 gctagagctt tgagtcgttg tctctgttgt tgagcttcca ggagtctgga catagctgga
     1261 agatggaata gttgttgatg gtgtaattgc attcgatcta gctgtatatt gatctttgta
     1321 gtacagttca ccgcccacac gaattaatcc gcttggcatc tcccagtctg tatttgtcac
     1381 gttagctgaa acatactgca taagctcgcg ataaacatca gaggctacgt gtgacgattc
     1441 tgaagtgaca ggtgtcattt ttttattgta acctgtccac acggaaatcg aataatttgg
     1501 cgtatagccc gcaaatagga tatctggata caccccagaa gaaataccaa gttttgcata
     1561 ctcgtcatct gtgtaattgg atgttccggt cttaccggct tgatataacc cagcgatttg
     1621 cgcattcgta ccagttcctt cagtgatcgt atcttttaaa atgtctgtga tcatataagc
     1681 tgtttctgga gacatggctg tttttccatc tggctcataa gtttcttctg ttccgtcttg
     1741 gaagacgatt ttattaacat attgaggttt gtaataagtc cctccattgg cgaaagccgc
     1801 ataggctgct gccattttta aggaagaagc tccgtatttt gtcccatctt gttcttccgt
     1861 attactagag atcgcattcg attgatggat cgtactgtat tcgatcccaa gattttttaa
     1921 gaaagaagcc actttatctg aaccgacttc attgaataat ttgactgctg gaacattacg
     1981 ggataagtaa agtgcttggc gtaaagtaat ggtgcccata tattgattat cccagtttcc
     2041 tacaggagtc gaagtccctt catagttata cggtgcgtca gtaatagttt ttccagtaga
     2101 atatttcaaa tattcaaaag ctggtccgta atctgtgaca ggtttcatcg tagaaccaaa
     2161 atcacgggaa gtattcactg ctaagttgtt gcctaaagtt acgtcttctg cgatgtgacg
     2221 tccgccaatc tgggctttta ctttcccggt attggtgtcg ataagggtgg aggcaacttg
     2281 catctcgtcg tctgggtaag agacatactg atccgtgttg acgatatcat atagtttctt
     2341 ttgggcgtcc agatccaaat tcgtataaat ctctaaaccg tctgtgtaga cattcttatc
     2401 tgttttttct tgtacttcat tgatgacttc tttgacataa ttatcgacga tcttagtatt
     2461 gtcatcggat tgagttagtt cttgcagacc atccgttacc ggtacattaa cagcttgatc
     2521 atattctgtt tgagagattt tttcgttttg aagcatcgta tacaaaacag tatctcgtct
     2581 tttcttcgcc tgatctggat aaacataagg atcataggcg gaaggggctt gaggcatccc
     2641 agcaagcaat gctgtttgcg gtaaagaaag ttctgataat ttcttaccaa agtacatctc
     2701 agaagctgtc tccatcccgt acaagccgtt tgacatatac actttattta cgtagtaggt
     2761 caagatctct tgtttcgatt ttttttgttc caatcttaca gccatccacg cttcttgggc
     2821 cttgcgtttc agagtctgat cctcggcact ggtggagaag aaggaaagtt tgatcaattg
     2881 ctgtgtcaaa gtacttccgc cttgcagtcc gccagaggta aagttagaga gggcagaacc
     2941 gatgatccga atagggtcaa ccccgatgtg tttataaaaa cggcgatctt ctaccgacac
     3001 gatcgcatct tctaatgttt tcggtaattc attcgcagaa attttttcac gcttttctgc
     3061 gcctaaatct tcgaatagtt cgccatcttg tgtatatagt ttggaagata cggtagcgtc
     3121 taatttttta tccgtcaatt cgggcgcatc ttttgcgtaa taccagaaaa gtcctacacc
     3181 tgctaaaaag aggatacaga aaaatgagag tattcctaaa aggatcttga taaataatcc
     3241 ttttttatgt gttccagagg actttccaga acctttgcca gaattcttct tcacactctt
     3301 tttaggagta ggctgtttct gtcgtctaga gcttctagtt tgttcatttg ccattcttgc
     3361 tttctccttc aatagataat gttgataaat agaagtcaac cgcgtccaaa taaggaatcc
     3421 gcggagaaat accagttttg atctctattc ctagttcttc gatcaaagat aaaggtagtg
     3481 atttgcgtcc tttttccttt tgggcgtccc agtaagcaat caaatgaaca ctattcaaat
     3541 aaaaacagcg cttcaaagac gaaaaccata acaagacaaa gcagacccca ttttgttcga
     3601 ggcatgcttt catatgttcg atttgatggg catggaagtt tttaaaggga aaagatgttt
     3661 tgttcttggt ttcttttgct tcaaagtcca agtaaaagcc tcgatagacg ccattatagt
     3721 ctgtagttga ggcttgggta aagtaggctt ccttgatcac agcagcactt ctcttaggat
     3781 agtctacttt cacgatttgg ataggggtag gctttttatg gataaccgcc aacccgcggg
     3841 aaagataata tttattactt tcattgattg cttcttcaaa gcgcatcccc cgattgccga
     3901 attcagtgat ctgatttctt ttttcttgaa ttttttcgac gggagaggca ttcttataat
     3961 aaggttggcc atttggatag cgcaatacca tctttaatca cactcctagg ccacattata
     4021 gcaaaaaagc ggatagaaag aaaaggaatc tgagatggat aagtcaaaag taatctatgt
     4081 gtctgggtat cgaagtttcg aactcggtgt ttttaaagaa aatgatccta aaatccaagt
     4141 tgttaaaaat gttttaaaaa aagaactcca tcagttagca gaagaagggc tggaatgggt
     4201 acttgtcagt gggaatttgg gcgtcgaact atgggctgtg caaatagtag cggaattgaa
     4261 agaagaatat ccggaactat ctcttggtat tttgtatcct ttttctgatt ttggtaataa
     4321 ctggaatgaa gggaaccaag aaaaaaaaca attagcagaa caattagcag attatgtcga
     4381 agctgttagc catcaaccct accaatcacc tagtcagcta aagaatcaca ctcgttttat
     4441 acttgagcat acagtgggct gtctgctgat ctatgatgaa gaattccctg gaaaaacgaa
     4501 gtattttttt gaagatgcac agaattacca agcagatcat ccctacgaaa ttcggctgat
     4561 cagtatggac gatcttcaaa attttagtga ataatcgata ttgtgtttga tttatcaact
     4621 tttttcctat acaatgaaaa aggtagaaat gttattacgt aaatgaaaac tagaggtgta
     4681 aatatggcga atttggttta tagtcctaaa gacattttac aacaagaatt caaaacaaaa
     4741 atgcgcggct atgatcccgt tgaagttgat gaattcttag acaatatcat caaagattat
     4801 gaaacttaca gcaaagaact ccttgcttta caagaagaaa acgatcgatt gagtgcaaaa
     4861 gtagctcaat tatctaaaac tcaaggagca gctcaaacac gcgtgcaaca aacagaggca
     4921 ccaaagagcg cagcagtgac caattttgat atcttaaaac gcttgtcaaa tcttgaacgt
     4981 gaagtatttg gtaaaaagct tgatcaacaa gcaagtgcag tcaaaccagc acaacctaat
     5041 ccaaataact acacaaatgc agacacaagc ttagatgata atgaaaaaac acgtcaattt
     5101 taatttttga ttaatgaatt tgcggttttc gggtaatcgc ggtctgactt gatcaggctg
     5161 aggaaagtcc atgctcgcac aggctgtgat gcctgtagtg ttcgtgctta gcgaaaccat
     5221 aagctaaggt actctttgga gtaacggcag gaaaacaagc taaggcttcg gctatgctta
     5281 agtatccttg aaagtgccac agtgacgaag cggctgggga aacccatccg gtggaacgcg
     5341 gtaaacccct cgagcgagca acccaaacaa tggtaggggc gctttttgtc tggaagaaga
     5401 acaggacaaa aaggcagtat aactgcagat agatggttac cgctttgttt tttctccctg
     5461 gaaaagcaaa gtacagaaca tggcttatag aaaaccgcaa acatcaggga attaggtcgt
     5521 gacaaaagta atgcgttata agaaacaaga aaaacatttg aaagctattt ttcaatactt
     5581 cagatgaatc gttcttatta caaaacaaac ttttgtatca ccggttattc ggaatttgag
     5641 taaaagtgtg gggtaggact gatgtcccac ctcctttttt tataaataag gagattgtgt
     5701 atggaaaaaa acaggaagtt caacctcgtc gcaactgcgg caagcgggct agaagcactg
     5761 gttggtaaag aattaagaga tcttggaatc gaatgccaag ttgagaacgg acgtgcacgt
     5821 tttgaaggaa caatggaaac gatcgctaca gctaatttat ggttacgtac agcagatcga
     5881 gtaaaaatcg tggttgggga attcgatgcg tatacgtttg atgaattgtt tgaaaacgta
     5941 aaagcattgc cttgggaaga ttttcttcca ctagatgcag cttttcctgt agctggaaaa
     6001 tcgatcaagt cgaaactata tagcacacct gattgccaag cgatcacaaa aaaagcaatc
     6061 gttgaacgtc tccgtaccta ttatcaccgt ccagcttctg taccactgac ggaaactggc
     6121 gcacattttc agttggaagt agctttgctg aaagaccatg tgatggtaac tctggatacg
     6181 acaggaccaa gtttattcaa acgtggttat cgcttagaaa aaggtggagc accattgaaa
     6241 gaaaacatgg cagcagcact tattatgttg acaaattggc gaaaagaccg tccattttat
     6301 gatcctgtat gtggttcagg tacgctttgt attgaagcag ccttaatagg ccataatatc
     6361 gcaccaggat tcaatcgtga gtttgcttgc gaatcttggg attggttcag tcaagaaatc
     6421 atggaaaatg ttcgatcagc agccgaagaa aaagcagatt atgatattga attagatatt
     6481 actggttcag atatcaatgg acggatgatt gaaattgcca aagccaatgc agaagaaatc
     6541 ggattaggct cttcgattac ctttaagcaa caagcagtca aagacttcaa aacggataaa
     6601 gaatacgggg tcatcgtggc taatccgcct tacggagaac gtctaggaga agaagaaact
     6661 gttcggaaat tatataaaga aatgggcgat gtattccgtc cattaaaaac atggagtaaa
     6721 tatatcttga ctagcgactt agcgtttgag gaattttacg gacaaaaagc aaccaaaaaa
     6781 agaaaattgt acaatggtgc attgcgtacg gatctattcc agtattgggg aactcgtccg
     6841 cctagaaaaa aagagaaaga cgaataatct tactaattga ttcattcaat tggatgtttg
     6901 gaggaatagg gaatgaaaga aaaagagttt ttacaagaag taaaagaaat caatcttcta
     6961 caacaagctt taggaatcct cgattgggat acacaaacag gtatgcctga aaaagctagt
     7021 gcataccgaa gtgaagtaga cagctatctt tacagcattt atttttcaaa aaaaatcggt
     7081 ccaaaaatca aagaagccat tcgctatttt tctgaacatc cggaagaatt gtcggaagtt
     7141 ggaactgccg tttttgccaa agtaaaagaa gagtatgatt tggaacatcg cgtaccggaa
     7201 gatcttatga gaaaatatac agctgccgtt tcttcagcac acggacaatg gcaaaaagct
     7261 agagaaacac aacaattttc tgattttcaa cacgcattga atcaaaatat tgaattaaca
     7321 aaacagttga taccttactg gagaaaagat gaaaagacga attatgatgt tttgctgaat
     7381 caatacgaac ccgggatgac tgtcgaaatc cttgataagg tatttgatca gcttaaagaa
     7441 ggaattcttt cgatccgccg tacgctccaa gaaaaaggga ctgaacctga aacagacttt
     7501 ttaaatcgga gaatgacgaa aaaacaacag aagagatttg tagtgaaagt aatcgaacaa
     7561 cttggttatg attttagtcg tggtcgttta gatgacactg tccacccatt tatgactgga
     7621 attaatccga atgatgcacg aatcacgaca cgatggaacg aaacagattt caaaatggct
     7681 gtttttggag tgatccacga ggctggtcat ggtatgtatg aacaagacat cgatgataaa
     7741 tatgcgtata caccggttta tgaaggtaca tcaatgggta tccatgaatc acaatcgttg
     7801 tttaacgaga tcattattgg aagtagtcgc agcttctgga aaaagcaata tcctttcttt
     7861 caagaatgtg cagaaggaac attcgatgat attccttttg acaaatttta tcgttcattg
     7921 aaccatacaa aatcaagttt ggttcgtatc gaggcagaca gtttgactta ccctctccat
     7981 atcatcattc gttatgaaat cgaaaaaatg attttcaatg aaggggtaga tgttgctgaa
     8041 ctacctgaaa tatggaatca aaaatatgaa gagtatctgg gaatacgacc aacaaatgat
     8101 gtggaaggca tcctacaaga cgttcattgg tcaggtgcaa gctttggtta ttttccctct
     8161 tatgcattag ggtacatgta tgcggcacaa ttacttcatt ctttgcagca agaaatagat
     8221 gtggaaaatg ttctagcgtc agacgattat tctccaatca aaaactggat gtcagagcat
     8281 atccatcaat atggtgcatc tagaaaacca aatcaattaa tcatggatgc aacacatgaa
     8341 ccgcttaatc cgcaatacct gatcgactat atgaaagacc tgtattttaa tgtatatcag
     8401 gtaaaataaa atacaattta caaagatagc tgaaattcat ctaatctata gatgttttca
     8461 gctatttact taagaaagaa gtggaacgaa tggataaatt acaagtgatc gctgattata
     8521 gtatggacca attagcgcat gatcaaacag gacatgggag cgatcatacc aaaagagttg
     8581 tcaagttagc tgaaaaaatt ttggagacgg aaccgcaggc agaccgcttt gtgacgcttg
     8641 cagcagctta cctacatgat acgattgatg ataaagtcgt taaaaatgag aacgaggcaa
     8701 agcaacagct tcgtgtattc cttcgctctt tgccaatagt agaggaacag atctcgatga
     8761 ttttttccat tatcgaaaat atgtcctttt ctaaaaatct ctctgaagcg gccgaactat
     8821 ctctggaagg taagatcgtt caagacgctg atcgtatcga agcattagga gcaatcggca
     8881 ttttgcgtac tgcttatttc ggcggtggac atgggcaccc gatttttgat cctgaactgt
     8941 atccgcaaac attcaaggac aaagaaaatt accgaaaagg cacgacagtg atcaatcatt
     9001 tttatgaaaa actgttcttg ctgtctgata aaatgaatac tgattacgga tatgaagaag
     9061 ccaaacgaag agaaaccttc atgcgtgaat tcttagaaga attttttctg gaatggtcta
     9121 ttgacgatga aaattttact ccagacaatt ggatcaaaaa taacaaaaaa tgatggaggt
     9181 tgtgacagaa gtggtcagct ccaagatatt tcggatcatt ttccgtaaaa gttacttctg
     9241 gaacactgtt taccaagagg tcgtgagcct gccgttcagc tccgagtcac aaggaacaat
     9301 ttcaaaaaat agcttctcgt gtttgatgaa tattgtaact tgtgattcgg aattggcagg
     9361 agaacaccgt ttattcggaa gaataggact gtgacaagac ttttgtcaca gtcctttttt
     9421 cgaattcaac aagtaaaact tttaagaaat attcagagag taaaattaaa aattgctgat
     9481 aaacgaagat ggaaatttta tgtattttta ttggtctatg caataaataa attaaatatt
     9541 cagaatacaa ttgattttaa cgaaagggct tctatcaatg gttgtgttgt atttatctgc
     9601 ccaggtagag taaaatagat cattatctaa aaggtaattg ttcatactat ataagtcatt
     9661 tgaaagtaaa aagatactgt aaataaaaaa tgtggttaaa taattctcat aatcagtgta
     9721 agcgttatca aaaatatagc atacttgctt tgtagatgaa tgacacgata tatgtttggt
     9781 atgttctgca agacagatga atatggaggt ggagacaatg tcttccaaag aactaacaat
     9841 gtatttagag aaagaattgg atcaactgaa agaaaagggg ttatacaata cgatcgacat
     9901 actagaaagt gaaaatggtg cttgtatcat cgtagacgga aaaaagatga tcaatctagc
     9961 atccaacaat tatcttggat ttgccaatcg cgaagaattg aaaaaagcat gtattgaagc
    10021 aaccgaaact tatggtgtag gagcaggagc agtccgaacg atcaatggtt cgctgaaaat
    10081 ccatcagcaa ttagaagaaa aaatcgcaga attcaaaggg acagaagcag caatcgcctt
    10141 tcaatctgga ttcaactgca atatgggcgc tatctctgca gtaatgacaa aagaagatgc
    10201 tatcttatcg gatgaattaa accatgcatc tattattgac ggttgccgct tatccggagc
    10261 aaaaatcatc cgaattaaac atcaagatat gaaagacctt gagaaaaaag caaaggaagc
    10321 gatagaaagt aagaaatata aaaaaatcat gtatatcaca gatggtgttt tctccatgga
    10381 tggagacatt gcacgattgc cggaaattat tccgattgtt gaaaaatatg gactgatcac
    10441 ctacgtcgat gatgcgcacg gctcaggcgt aactggaaaa ggtgcaggaa ccgtcaagca
    10501 ttttggatta agcgacaaaa tcgatatgca aatgggcacg ttatcgaaag ctataggtgt
    10561 tgttgggggt tatgtagctg gatcgaaaac actaatcgac tggttgaaag ctagaagtcg
    10621 gccattccta ttttctactt cgctaactcc aggtgcagct gcagctgcat tggcttcgat
    10681 tactttgatg caagaacatc cggaacttgt cgaaaaagtt tgggaaaatg ctaattattt
    10741 taaagaagaa ttgaaaaaag taggctataa catcggtatg tctgaaacac ctattactcc
    10801 agtcattttg ggagacgaaa aagtgactca gactttctct aagaagctga tcgaacatgg
    10861 gatttatgct aaaccaatcg tttatccaac tgtgcctctt gggacaggca ggatcagaaa
    10921 tatgccgact gcggaacata cgagggaaat gcttgatgaa gcagtagcag tttatcaaaa
    10981 aataggaaaa gaaatggaaa ttatttaact aaatttagtt ttttcgtcta tttaaaatta
    11041 gagaacatgt tcacgcatat attgttaatc atctgaagat agtagtaaaa aagatcaggg
    11101 aggatcatca tgaaaaaagt attggtgaca ggatgtttag gacaaattgg ttcagagttg
    11161 atcacacggc tacgtcaaga actaggcagt gaaaatgtca ttgcgacaga tatacgcatg
    11221 cctgaagtca ttgagaaaaa cgatatcttt gagatcctag atgtaatgga ttacgaaaaa
    11281 atgcgcagca tagcagaaca atatcaagtg gacacattga ttcatttggc tgctcttttg
    11341 tcagctgttg cagaagcaaa accacaatta gcctggaatt tgaacgtgac tgggctggtc
    11401 aatgcattgg aagtcgctag agaattccag ctgaaatttt ttacgccaag ttcaatcggc
    11461 gcgtttggtc caaatactcc aaaagatcat accccacaag atactcttca gcgtcctaca
    11521 accatgtatg gggtaacgaa ggtagctgga gaactactat gtgattatta ttatgtgaaa
    11581 tatggcgtag atactcgtgg tgtccgtttc cctggattga tttcttatgc aaccttgcca
    11641 ggaggcggta cgacagacta cgcagtcgat atttattatt cagcaatcaa agaaggaaag
    11701 tacactagtt tcattgatca aggtacatca atggatatga tgtatatgcc agatgcgatc
    11761 gatgccatta taaaactcat gaatgcagat cctgatcgat tggttcatcg aaatgccttc
    11821 aatatatcgg cgatgtcttt tgacccggaa gagatcaaag catctattca aaaaatcata
    11881 ccagattttg aaatggatta tgatgtggat cctgtacgtc agtcaatagc agaatcttgg
    11941 ccaaattcac tagatgtttc ttgtgcgcaa gaggaatggg gcttttcgcc gaaatacgac
    12001 ttagatgtga tgacaaagga tatgttggaa aaactgaaag aaaaattggt agagacggta
    12061 taaaacaaga accacatcca aaaagttttt tatacttttg ggattggcta tggtatgaaa
    12121 ggggacaagg gatggaagaa tatcaagcca cgccagtaaa agaagtaaaa atagaaaatg
    12181 aattaaaacg tacgatggga ttttttactg ctttatcgac tgttatgggc acagtgatcg
    12241 gtgcaggggt attttttaaa gccgcaagcg tagcagaagt tacaggatcc gccagtttgc
    12301 atatgttttc ttggtttctc ggtggtatga tttctgtctg tgcaggacta acaggagctg
    12361 aactggcagc tgctattcca gaaacaggag ggatgattaa atatatcgaa cggatttacg
    12421 gaaatacagc agctttttta ttgggctggg cgcaagtcgt gatttatttt ccagcaaatg
    12481 tagcagcctt gtcgatcatt ttcggtacac agttcgtcaa tctatttggt ctttcccagt
    12541 caatgattgt tcccgtagct gttacagctg ctgtatcgat cctgttgatc aattttttag
    12601 gttctaaagc tggaggggct tttcagtcga ttacgcttgt ctgcaagctt attcctttgt
    12661 ttgtgatcgt cattttcggt ttgttccgac aagaaggagt agacttccaa ctgtttccga
    12721 tccaagcagg agaaaatctt tcatttttct cagccttggg agcgggcttg ctagcaacga
    12781 tgttcgcata cgacggttgg atacacgtag gaaatatttc gggagaattg aaaaagccgg
    12841 cgaaagattt gccaaaagca atttctttag gaatcatcgg aatcatgatc gtttatttat
    12901 tagtaaatgc ggtgttctta agaaccgcat ctatcgacgg agtcgcagga aacagcaatg
    12961 cggcaagtga cgttgcaaaa atgatttttg gcggcttcgg tggaagactg gtcacagttg
    13021 gtatcttgat ttctgtttat ggaaccatca atggctatac ccttactgga atgcgtctcc
    13081 cttatgttat ggcaaaagaa aacaacctgc cttttagcaa gctctttgct aaacttcatg
    13141 ataaaactaa agttcctgta gccgcaggta tcttagagct agtcatcgct attggcatga
    13201 tgatgatcgg tggttttgat acactaacgg atatgctgat ctttgtcatt tggatttttt
    13261 atacgatggt ctttgttggt gtcattcttc ttcgtaaaaa agaacctgat ttgtttcgac
    13321 catacaaagt tccaatgtat ccgtttattc cactagtagc aatcattgga gggactttta
    13381 tcgtatcaag cacgcttatc actcagacct ttttagcttc gatggggata gtattgacac
    13441 ttgctggtat cccgatttat ctctatttaa aacgtagata gctaaaacat tcgaatttta
    13501 ttagaactgc acttctctgc caaacaaaat attttattta aatgaggagt acgtggaaga
    13561 aatgtcaaaa gtactcgtta attttgatca cgtaaatgtc gttttgtcta cgatgaaata
    13621 gcaaagtaaa agacagaaat cgggctcaac cgatttctgt cttttacttt gatttgcgta
    13681 actgaactat tcggcgtacg ccgcgataaa tatttaataa aaccaagcaa ccagaaatga
    13741 aaatgggaag ccaaatcaga tagacagggt agccaagcac tttagacaaa gcatgtggag
    13801 tcattttatc catttcaaga agtgcttgct gccaagtctg aagattgaag actagcatga
    13861 aacccgaaat aatcaggaga ataaatccta aataatgaat tgtagttctt tttcttacat
    13921 aagcgatcgt agcataaagg aacagtaaaa aaataggtac gccaaaaaca aagtacatat
    13981 tcagcatctc gacaccccct ttgtgtttat cttactttac tttgaaagaa atgagaaata
    14041 atagcctcgt tacttgcttg aaacagcaaa ggactgctaa gataagagag atacaaagaa
    14101 tcaaggagga gtcatatggc aagttatatc gaaccgattc gttttgcgct gattgttttt
    14161 ccatttttag ccttagcgat ctcatctgtt ttttttattt atgaatatcg aaagtatgga
    14221 acatttctag taacaagggc agttattttg tattctttcg ttttttattt attgtgtgcg
    14281 tattttttgg tgatcttacc acttccggca aaggagacgg ttgcccaaat gacagggcca
    14341 aagatggagc tgcatttatt tgcttcatgg caacatttca gagacaacac ggtattggtc
    14401 ttgtctgaac ctagtaccta cctgccggca atgaagcaga gtgtattttt ggaaccagct
    14461 tttaatgttt tattactcat accatttggc atttatttaa gatattattt ccggctttct
    14521 tttatgaaaa cgatgattgc tagtttctgc ctttctctat tttttgaatt gacgcagcta
    14581 agtggacttt attttatcta cccaagacca taccgcttat ttgatgtgaa tgatttagtg
    14641 acaaatacat tgggtggaat cattggtttt gtcgttacgc cgttatttac atttatgctt
    14701 cctacaagaa gacgaatgga tgaactttct tatgaaaaag gaaagactgt ctcattgact
    14761 cgacgcttgg tcgcttattt gatcgactgg acatttttat ctcttattaa tttggtagcg
    14821 gcgattttct ttaaaattgc gttcgattca ggggcagtta cttctcatat ctggtggctt
    14881 ttattagaag taggggtata ctttatgatt gtaccgttta taaccaatgg atggacactt
    14941 ggcaaaaaaa ttgtgcgtat ccgtttggtg gaagaagacc gcgaacgaat ttctttaaaa
    15001 gcattgatga tccgctacgg gtatctgtac tttctttttt atggtttaag ctggttgggg
    15061 cagttgcttc agcaaatcat gaattcgaca acgaatacgg tgttgcagat gagtgcagta
    15121 ttcatttctc tcggtgtctt gttactacaa ggtattttta ttttaaatat cttgctatca
    15181 tttttgagaa aaaaacggat tctgttttat gaaaaagcta gtcacacata tactgttagc
    15241 acgattggcc aaacaaagga tgatgattct cgaaattagc tgagcaaaaa cgctttgtct
    15301 gtgttataat gccaaagtcg tgataattta aagagaagag gtgcacgaat gaataatttt
    15361 gatatattcc gcttcaaacc gtttatcaat gaagcgttaa ccgcaaaggg cttcaaacag
    15421 cccacagagg tccaggaacg tttgattcct ttaattgcaa aaggaaaaaa tatcattggg
    15481 caatctcaaa ctggtacagg aaaaacacat acgtttttgt tgcctttgtt tgaaaaaatc
    15541 aatgcagaaa aagaggaagt acaagttgtt attacagctc caagtaggga actagctgca
    15601 cagatctatg aagcagctac acaaatcgct aaattttcta caccggagat ccgtgtctcg
    15661 aattttgtcg gcggtacaga caaacagcgt caattaaatc gtctgcatca ccaacagcca
    15721 caggttgtag taggcacgcc tggtagaata ttagacatga tgaatgaaca agcgctgaag
    15781 attcacaccg catttgcatt tgttgtcgat gaagcggata tgacattgga tatgggtttt
    15841 ttaaatgaag tagaccaaat tgcaagtcga ttaccagaaa agctgcagtt tttagttttt
    15901 tctgccacta ttccagaaaa acttcgtcca ttcttacgaa aatacatgga aaatccaatc
    15961 attgaagaaa tcaaaccaaa agcagttatt tctgaagaca ttgaaaattg gctgatttca
    16021 acaaaaggta aagatgagaa tcaaatgatt tatcaactgc tgacaattgg tcatccttat
    16081 ttagcaatcg tatttgccaa tacaaaacag catgtggatg aaattgccga ttacttgaaa
    16141 aaccaaggct tgaaagtagc aaagattcat ggagacatca ccccaagaga gagaaaacga
    16201 gtgatgcgac aagtacagaa tctggagtat caatatgttg tagcgactga tttagctgct
    16261 agagggatcg atatcgaagg tgtctctcat gttattaatg cagaaattcc agaagatcta
    16321 gacttcttta tccatcgtgt cggacgtact ggacgtaatg gcttaaaagg aacggccatt
    16381 acgttatatt cgccaaatga tgagcaagcc attgatgcga tagaaaaatt gggcgtctca
    16441 tttgaaccaa aagaaatcaa aaacggggaa atcgtcaaaa catacgatcg taatagacgt
    16501 gccaaacgag aaaaatcaaa agatgtatta gatcccacgc tgattggtct tgtgaagaag
    16561 aaaaagaaaa agatcaagcc tggttataag aaaaaaatcg attgggcaat tgcagaaaaa
    16621 aataaaaaag aacgcaaaat cgaacgcaga cagcaaagcc gtactgctcg taaaaataag
    16681 aaaaattcac ataaataaaa aacaagaggt tgtgacaaaa ggcgtgtcac aatctcttgt
    16741 ttttaagcta gacttttttt cattccgata caaagtactt catcttcaat aaaaggttct
    16801 gtgcaagtat gatacccttg tttttcataa aaatctacag ccgtcat
//
LOCUS       DACCKU010000065        16799 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.068, whole genome shotgun sequence.
ACCESSION   DACCKU010000065 DACCKU010000000
VERSION     DACCKU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.068"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..466
                     /locus_tag="IVN13_001981"
     CDS             <1..466
                     /locus_tag="IVN13_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7012451.1"
                     /translation="LFAKGDSQITARKFQNKMVKLNKFLIMAEEYSDSSWNAANLTSE
                     DCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQHSEMAKQADMCLVISQEEYDFA
                     KVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNKQELMQKGLLKEYRKT"
     gene            complement(618..1460)
                     /locus_tag="IVN13_001982"
     CDS             complement(618..1460)
                     /locus_tag="IVN13_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7012452.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(1552..3429)
                     /locus_tag="IVN13_001983"
     CDS             complement(1552..3429)
                     /locus_tag="IVN13_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7012453.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(3426..4847)
                     /locus_tag="IVN13_001984"
     CDS             complement(3426..4847)
                     /locus_tag="IVN13_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP7012454.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(4850..8239)
                     /locus_tag="IVN13_001985"
     CDS             complement(4850..8239)
                     /locus_tag="IVN13_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP7012455.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            8496..9887
                     /locus_tag="IVN13_001986"
     CDS             8496..9887
                     /locus_tag="IVN13_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7012456.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNGRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSNLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(9960..10607)
                     /locus_tag="IVN13_001987"
     CDS             complement(9960..10607)
                     /locus_tag="IVN13_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7012457.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(10615..11301)
                     /locus_tag="IVN13_001988"
     CDS             complement(10615..11301)
                     /locus_tag="IVN13_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012458.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(11307..12380)
                     /locus_tag="IVN13_001989"
     CDS             complement(11307..12380)
                     /locus_tag="IVN13_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP7012459.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            12602..13276
                     /locus_tag="IVN13_001990"
     CDS             12602..13276
                     /locus_tag="IVN13_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7012460.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            13281..14621
                     /locus_tag="IVN13_001991"
     CDS             13281..14621
                     /locus_tag="IVN13_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7012461.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(14770..15756)
                     /locus_tag="IVN13_001992"
     CDS             complement(14770..15756)
                     /locus_tag="IVN13_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP7012462.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            16480..>16799
                     /locus_tag="IVN13_001993"
     CDS             16480..>16799
                     /locus_tag="IVN13_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP7012463.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYG"
BASE COUNT         4841 a         3141 c         3033 g         5784 t
ORIGIN      
        1 tctttttgca aaaggtgatt ctcaaatcac ggcaaggaaa ttccagaata aaatggtcaa
       61 gttgaataag tttttgatca tggctgaaga atacagtgat tcttcttgga atgcagccaa
      121 tttgactagc gaggattgcg ccatttttat tagctacagc ggtcgcatcc atcattatga
      181 acgaataatg acctatttga agcatgtaga ggcccctacc ttgctgatta ccggaaatca
      241 gcattcagaa atggccaaac aggctgatat gtgtcttgtt atatcgcagg aggaatatga
      301 ttttgctaaa gtagcgactt tctcctctca aattgccttc gattacgtat taaatacgtt
      361 attctcagtg atatattctc aaaattttga agagaatatc cgcaacttga aaaacaaaca
      421 agaattgatg caaaaaggcc ttcttaaaga atatcggaaa acttaagaaa agatcctcat
      481 ttagtaataa gaaagatagt gtttgatagg ctctctttga tatcatcata aaaacaggct
      541 gaagttttct ttttcaaacc tcagcctgtt tgtttacgga acgattcgaa tagatatatt
      601 atagaaatga ggaatagcta acttctgttt cttattttga acgattgacg aaaaagatat
      661 ataagaagga ggaaaatcag tatacatgca gctaacaatc catacaggaa aaatcgtttg
      721 ttctcggata ctttctgtac ttctttttga acttcttttg catgataagg aacccggtgc
      781 cctctgacaa gtaagcgatg actgttgacc atataaggtg tacaagttag gagtgtcacg
      841 aaatcctgcc ctttttcgat tctcagatct tttgtatcag tcggttctac cgtcttgatc
      901 tgatcgactt gataagcgag tgtccgtcca ttgatttcga tatagaaaga atcacctaaa
      961 gtcagtttcg gtaagtctgt gaacaacttt gcttgaggca gtcctcgatg gctggaaata
     1021 acggcatgtg tattttctcc ccccgtagga taagacgttc cttccaataa tgaggcacct
     1081 ttttctagca gcacagtatt cgtttcatca aaaattggca aatggacatt gatctcaggg
     1141 atcgtcaaaa taccgatcgt atgtttctca aaataggttc tatccttttt cgcaggttct
     1201 tttttcttag taaagggatc tgcacctgga tttccacctt cttttgctaa ttgctggttt
     1261 ttctttgtca tgttttcttg gattttagcc atgacttctt cattttcttt gaccgcttgt
     1321 tgctgataat gagagatgat ctgctgatcc agataattat tcagcgcgtc gctaacaaat
     1381 ggataagcta gtatccctat tcctgagaga agcaagaaaa gcatcagcag atctagaagt
     1441 ttccttttct ttggtttcat ttgtgttctc cttgtgatga ctgtttgata ataaaagaaa
     1501 aaggagcatt tgcgtaatcc gctgctcctt tttccaattc ttgcaaaaag actaaatctg
     1561 actgtgcttg cgtctagcaa agtacagtcc agcaatcagt agaaggacta ctcctgcacc
     1621 gatatacaca tagattccct taccgcctgt tgaaggaagt gtacctttgt gtttatttgg
     1681 tattttttca ggagaaatca actgtcctgc tgttacataa gattgttcat cgatagtaaa
     1741 tgctacacgg tttgttaatg gaacatattt ttctggcgct ttcgtttctt ccagatagta
     1801 cgtgccatat tttagacctg tcacatcgat taaaccgtta cttgtggttg taaaaaccgt
     1861 tgctgattct ttcgccgata cccagctgac ggcttttgtg gatgggtcga tcgataaata
     1921 tttcgctgtg tcactatctt gatcacgaac gacgaattct gctccagcaa gtgtttggtc
     1981 tgatgtaacg tcaccatcta ctttaacaaa tcgtttgccc ccagtaacga catcgactga
     2041 cggtggtgtt tgatcatttg tatgcccgtt atcgacattt gcttggttag aaaatccttt
     2101 ggttggatct gctttttcgt tcaaatgcat atagtaaacg aatttcaatg taccgccagg
     2161 agttaatgaa ggaatataat tcggatcaac tgaaaccgtg aatccgtctg tttgctcagt
     2221 gacagaataa tttactgggt cgatttcttt atttccatca tataaggcat aagcatacgt
     2281 tcctgaagaa tcattatcaa atgttaaagc agcgtcatga gtatcgataa gtttgaatgt
     2341 tgtgtacttg ttttgcgttc cttctttatc agcgatacct aatgggatat tgacagaaat
     2401 ctcatattga attttttcac cgattgcgac atcttttcca ttcaattgag gtgttgtttt
     2461 atcaacttta gacgtatcat ttttgatggt cttttctact ggtgtttggc tatttgccag
     2521 gatctcaaaa ggcgtgattg tttgcttttc aatcatttcc gcattatcag gagcttttac
     2581 ttcttctaaa ttatattttc caacttctaa atgattaacg atcaactggc ctttttcaat
     2641 agatgcctcg gcaaagtcat tgttgccgat gtcataagaa tgaccagtaa tgaaatgttt
     2701 ggctttggtt tgatcagttg tccaagtgta caatccatct gtgacacttt ggatgtattt
     2761 tttgacgctt ggtgttcctt cttctttaga aataataaat tctgctccat ttagtgcttc
     2821 gttttcggca gtaccgattt ttgtaacttt caacgttcca tcattaccga ctgtattttt
     2881 agggtagaga tggatagtat ctagttcttc tgtcccgtat ttataagagc catctgcttg
     2941 tttgatcatc tcatatacag ggaaagctaa aaccatgttt gcggcagctg acactccgtc
     3001 ttttggttct tctttgatcg tgtagactgc atctttccca ttttgttttt taggtaaaga
     3061 taaagtgaca tttccatcag catctgtcgt tcctgaagca actggtgtac caggagtcaa
     3121 agactggact gcttgttttg ctgcatccac ggacgctcct ttatctcgtt gcgcataaaa
     3181 ttcttgagtg acgttataaa ctgaaaatga aatatcggct aatccttggt attgatcgaa
     3241 ttcgctcatt tctttcccgc tgttttggat taaaggatcg ggtaaatcag tcattttctt
     3301 tttatgaagg atgacttgag ctgctgtctc ctctgcaaac actttttttg cgccgaagct
     3361 gtttgtgagt aatggtaaaa taaggaaaag gactcctaac ataacgttta tttttttatg
     3421 atttttcatt ttttcactcc tgttcttttt ttgataaaca accagattgc aaagcttgcg
     3481 aataccatac caacaacgcc taaaagtgta tttttcgttt cgtttgtttt tggaagaaat
     3541 ggacggctat tttctttttt attgactact ttcaatatac tgtccgcctc tttatcctca
     3601 gaaagaaaag ttgaaatggt aaacggaatt tctgtttcac ttaaaatata accagaaggt
     3661 gcttcgatct cttttaatcg ataacttcct gttttcaacc cggaaattga aaaagcgcca
     3721 tttttatcgg aaatcaggtg aagcgcatct gattcgttct ttgtccactt gtatccgttg
     3781 gctgtttgct ggaggtaatt tcctgctttg tttttaataa ggaatactgc gccagctaat
     3841 cgtttatctg gtttatttgt ctcgattttg acaaattgct tactccctgt atatacagag
     3901 actttctttg tactgacacc aaaaccaaaa tccagtgttc cgttgttgtt gatttccttg
     3961 ttcgcctgtg ctgtactgct tagctgcatc tgataagaaa tggtcagttt ttttccagca
     4021 tatttttcca acgaagggat cgaaaataaa acatcaaatc cgtgaggttt cttgtgtatg
     4081 acaaagcctt ctgtcagctt ttctccattc gatgaaatcg ttaaactatt aggtaaaaac
     4141 gttaatgctt catccgcact atctgacaat tcgaactttt gatagtcaag gatatttaca
     4201 ggaattgtcg tatgcaagga ataagtgatt ttttcaccaa tcgtaaaatc atttctaggc
     4261 tcttctaata ccttctcaaa aggtggatca gggtagtcgt tttcttcatt tttaggatac
     4321 aaatggatag ttgaaagttt ttgattgttt tgtccatgta caggaagaac aactaccata
     4381 ttctctgcct tttctttgac gacttctggt actttggatt caatgaaaag atacgcttta
     4441 tctctttttt cttgatcttt agcggccaga gaaaaagaag cgataccatc ttcgttgtta
     4501 agagttgtag ttgttgcttc tgcggtaaac ataccggaag acgcaccgtt ttttgcgatc
     4561 tctgcttgtg cttcttctac cgttttgccc ttttcccgta gatggtaaaa agaatctgtg
     4621 acatcataaa cttggaatgt gacaccattt aatcctcgat acgtttgtaa taaagctttt
     4681 tcttgtccgt catttggatg atttttcggc agttgcccgt ttgggaaaag caatttgtgt
     4741 aaagtaatct gaaccatttc tgtttctgtt tctgttgcta cctgagtaaa agctggttta
     4801 aatagtaaca agaagagaca catactaagc caaccaagtt ttttcatatt tagctgcccc
     4861 ctctttcttt tttcaaaaaa agataccaaa tagaaaacgc acaaccaatc atccctacta
     4921 gatagattcc taaacggcca attcctcccg tttcaggtaa tggtactttt gcctgattcg
     4981 tgatgtctaa agaaatctgg ttgtttttgg ctcctggtga cagaacagat tcatgatctt
     5041 gtccatccac ttgaattgac ccatcttcgt gtataactat agtaactggc tcttttagac
     5101 cttggtatcc ttctggtgta aaagtttcgg tcagcgtata agttccaggt tttaaatttt
     5161 cgaatagaaa ggtatctgtt tcttgtccat cttttggcga ttctaagtcc atttctggtc
     5221 cctgcagtct gaattttgct ccttttaatg tctgtccgtt gtcttctttt ttgtgtaccg
     5281 ttagatcaaa cgcttttagt cgattttgat gagtgagcgt ccatacatta tttgtcatct
     5341 cggtattttt tcccgtgtaa atgatttttc catatttatc gatttggaat tcataatttc
     5401 ccggcgaagt atcatatccg gcaggaccag ctgtttcaac gactcgatat ttaccaggtg
     5461 tgagtgaatc aaaactggca agaccttttt cattcgttgt ttggctgtca attggctgat
     5521 aagtaccatt tgcttctttt ttctgtaggg aaaatgctgc tcctttaaga tttatctctt
     5581 tgtccgtccc ttgcatcgca tacttacgta ctgctacagg aacttcaaca aaaggatttt
     5641 caatagtcaa ctggatcgta tcatcggaag tagtgactgt ttttccatca atggttaccg
     5701 taccatcaga agcgatcttg atttcccaag tagtcttttt gcttaatcca tgcccttctg
     5761 gagcttttgt ttctgtcagc gtataggtca tttctttttg caatttgaca ttttctggaa
     5821 gagaataggt tccgtcgcca tgatctgtta gtaaagtatc aatagaatct cctgttaatt
     5881 tgaaaacagc gccaataaga tccttttcac ctgtagagga atttttcgtt attttcaagt
     5941 ttaacggtgt gaattgttta gtatttttcc atgtcattgc atctatttgt tgagaatcgt
     6001 atcctggtac aggtaattct ttgattgttt ggtaactgaa tgcttgacct tgattattgt
     6061 attgaggtag tgataatatc tcttgataac tttctccgct atttgcagat aatttgtcaa
     6121 tgtctgcacg ttcccacgta tttgttgtat ctttagctgg tttaatgatt cgaatatatc
     6181 cgtttttcca agctgcagca tttgtcgtat gttcccgttg aatctcaaaa gtaacttgat
     6241 ctggacgatc agctagattg ttgtcaaatt cttcccataa ctttttgatg tgaagactga
     6301 ctccgggagc tttagcagat ggtataccga attcagctaa ttcattggta tcaatgtttg
     6361 gctggaaagt tgtcctgccg ttcatttgat accagaaatt tggatggaag tcctcatttt
     6421 ctgtttggat tctcacttgg taatggattt ggatttcttg gtcttttcct aaatagatct
     6481 gattgctctt gatggtattt ccttctatgg aaatagatgg agtgaccgtt gtaggacttg
     6541 tccccacact cttgactgaa agtgtaccag gctgataaat gaatggttct gcgattggat
     6601 catttatttg tccattgctt acagttgctg agatctgtac agcttttttg gctaggtatt
     6661 cagagatatc tgttgcatga tcagctgatt catagtaaag atcgcctttt tcatctgatg
     6721 aaaccatttg tcgcatacga gactctactt ctgcttttga gagaccagct gccggatcgc
     6781 tttgaagttg gatgccaaga ccatgtattt cgattcctcg ttctttgagt gccatcgctt
     6841 ctccgatggt tgcgataaac gtactgtcga ttcttctgga tagattgttt tggtcaggtg
     6901 catcatagat tcttgagatt agagaagtat ttcccggccg atcttgcgta ttagaaaact
     6961 gagttccgta ataattgctg cttgattgtg cgtgtacccg ctgtacttta taggaaaatg
     7021 ttggtacacc atccgtcagc aaaacgatca cttttttatg tccatttgga acggatagca
     7081 tgcttcctgc atctcttagt gctttttgtg taaaagtacc accatttgtc cgtgaaggtg
     7141 taatggattt tacttgattt ttcactgaat caaatgaacc catctgtact gcaccgttac
     7201 tgtagctata tccttcgctt gagtagccga catatcccat attgattttg tctgtgatac
     7261 cgctatctgc taaagtatcg acaaaacgat cgacaccaat ctttacttca ccgatccgat
     7321 tattgtcgtt catacttcct gaccagtcta cgaccaatac gagatcaaga ggcgtgatat
     7381 ctttttgtac atttccacga gcattcaaat aaacatcaaa caatccgggt gtacttgttt
     7441 cctttgcata ctttcgtatc gcatagtctg cttgattacc ggttcctcca tattcgatat
     7501 acgaatgggt ccgatcatta ggatctccat tccaagaatt tataccatcc cattgatttg
     7561 ttccgttttt attgccttga tgatcaagaa catttgtatt tcctgtaggt tgccaactgg
     7621 ctttaggata agtgccctga tcatctgttg tatattctgt aggaatcgtt tgattagatg
     7681 gcacactcac agctggcaag gataatgtag gcaatgtaaa tgggctgaca gaattttcgt
     7741 tttcttttga tgattcttct tctatttcgt ctggctttgg tactgacaca tctgtatctt
     7801 tcttattttc tgtctcatca ataatgtcat attctttatt atcgacgatc agcttgtttg
     7861 cctggctgtg gtaaagtgct ggatcgattg gaaaactcag atcgatagtt tgtttagtgt
     7921 ttgatgagat ttccaacgta attttcccgc tatttacctg ataagcatag ccatctcctt
     7981 cttttctttc cacatcactt aagtaatttt cgactagacc aatttctttt tctatctttt
     8041 cagcactggt gttattcgca tttagcttga tgttcagtgc tttttttcca gactgatccg
     8101 aaatgacttc agctgaaatt gtttctaaaa ctgttggatc atctgtagtg tctgctattg
     8161 cagataatgg cacaaagttt gataaactca atattatcag catgaaaata actttcagtt
     8221 tcttccctgt ggtcgtcatt tttcccctcc tttttctttt tttctgtttt gacgtgaata
     8281 gtgaaaatta ttaaaataaa caggcgttac ataattttat ttattttaat atgataatta
     8341 ttatacgaag ggcgctaaag ggaatcacac caattttcat accaattggt ttcatttatg
     8401 aagttgattc cttcgattaa tgagcgtttc attgaaagaa aagggttgag ggagaaaaac
     8461 atatgatcag gttgttggaa cgaaactata gaaagatgga aaaaatcatt cgaattctgt
     8521 ccatttctga caagtggatg actaaaaaag aacttgccaa acgaattggt agttcagaat
     8581 ctacatttgt aaggtatatc gaagagatca aaatccgctg gggaacggat gtaacgattc
     8641 aatcttcaca aaaattgggg tatcgtcttg aacatttgaa tgtaccagct tatttaaatg
     8701 ttctgatgga aatgtcgcaa agttctacaa acggtcgttt attaaatgag attattgtca
     8761 atccgggtaa aacgattgag tattattgtg aaaaaatttc catcagccgg tcttctttta
     8821 cacgtaagtt gaaacaatgc aatcaaattt tagaaagcta tttattaaaa gtgatcgttg
     8881 accaaggttt tcagttaact agccttaaaa gtgagctcca gttgcgtatc tttgttacct
     8941 ttttcttttt aagttactac ggaagacgca cgttgccatt tccaatcgaa aaaaagaaga
     9001 tcaaagaaat tatggtcaag aataattgtc agatgaattt gtttcaggaa gaaagcccat
     9061 atgagcaatc tttctttatt atgtattttg ttgtgcatct catgagagaa tcccaaggaa
     9121 ctccatttga atggaaagct ggagactctt atgtacagtt gataaatatc gataaaaaag
     9181 acttcttaat cttgaaaaat ctcttaggaa aattgaagta cagcagctat ataaaagtag
     9241 tgaactattt tgtcaatacg attttttcag aattttcgga gaaagaaaaa aatgaaatca
     9301 gtgagcagat acgaaaaaat atgtcctact gttcgttttc atccgctgat tgcaaagaaa
     9361 aaaacgaaga atatgttgtt caattattaa cgcaaattta ttttttctcc cgtattttcc
     9421 catacgatac gactcatttg acgatccgtg caacttactt tgcacagcag atgcaacacc
     9481 gccagatacg tctatatgct ggatttcgtg cagaacttca attttattcc aatctactga
     9541 atcagaatct gatttattat ttatctagct atatcttttg gatgcttatc ggtaatcctg
     9601 aaattcacca ttatacttct gataaaaaaa tactgattat cagcgactta agtcttcgac
     9661 atagtcaata tattgaagaa tatatcagtg atattcttgc tgtgcataaa attcactctg
     9721 agacaactgc tattactgaa gatcagctgt cgaaatacaa tcttctcgag tatgatctga
     9781 tcgttacgaa tcaacccatt ttgaatgccc atgttcctca tatattgatt gatgatagtg
     9841 tgtcattcgc aaatgaagaa gaactcatcc gcttattcga gttatgaata atcaaaaaac
     9901 agctatgtat aaaccaccct gataaaaatt tcaggacaaa tacacatagc tgtttctttt
     9961 tattgtattc cttcagcaat ccaacgagat ttatagcgaa agtcttttaa caactcttct
    10021 tttgtccaat tatttgcaat gagatccgtc aaagtgttca ttacgagggt catggaaaag
    10081 tataggaaac taactgcttc ttccgacgat tgaatggaga aatgattgat tcttaataat
    10141 tccagccatt tttcgttcat tggagaatca gccgatagtt ctctacgttt ggttgtctgc
    10201 tgatcatttc gctgggttaa caagtataac ctttgccaat agatactttc gtgatctaag
    10261 tcgcagcacc actctaaata tttttcgatc cccaaaaaga aatgttgttt gttttcgcta
    10321 atgaatttta agatatcttg atggactatg tgagaactgc ttttgatcgt atacaaataa
    10381 gcatcttcca aatcttgaaa atatttataa aaagcaccgc gcgacatccc catcttgtca
    10441 acgatttctg agactttgac ttggctgatg tgctgtgagc aaaatatctc cagtaatagg
    10501 tcatcgattc tctgtttctt tgcgttcgat aaattgaaaa aagtttcttt tggcatccac
    10561 atctctcctt tatctctgat tatacgtgga tagcccttca ttgtcatgaa ggattcattc
    10621 cttctttttc tcttcttctg tcaagtaccc atcttccatt cgataaacgc gatcactcca
    10681 ttgaatcata cggacatcat gagtaaccat aatagtcgct ttttgtttct catgagcttc
    10741 tttaactagt aatttgacga cttcatatgc atgatctgta tccaaactag ctgttggttc
    10801 gtctgctaag atcaagcttg gctcattata taaagcgcga gcaattgcca ctctctgtct
    10861 ttctccacca gaaaggtctt taggataact atttttaagt gactggatat ctaaagattg
    10921 cagcaaggaa tcgatttttg ttgaatcttc tttttttgaa gtaacttggt caactagttc
    10981 aaattgttcc tttaccgtta aaaaaggaat caaattagag ctttggagaa taaatcctat
    11041 ttctttaaag cgtaaatctg ctcgtttttt ttctggaagc tcagttagtg agtgaccgtt
    11101 gatcactatt tctccggaag tcggtgtttg caatcctcct gaaatggtaa ggaaagtact
    11161 tttacctgaa ccagaaggac caatgatgct gacgaattct ccttccttta cataaaaatc
    11221 aattgctttc aaagctgaga cctttgtatg accagaacca aatgttttgg taactttttt
    11281 catcgttaat atttttttca tttttattaa cctcctattg ctgtaattgg gtctactttg
    11341 gttatcgtac gtatcgaaaa caatccgcct agcattgcga caatgatcaa aatcccacta
    11401 tatagcaacc attgtgacca aaaaacagca aaaggcatcg cgcttggtaa aatcagacta
    11461 gtcaaataag caaacaaaag agcaagcaga acgcctacca ccccaacaat aaaggactgt
    11521 gctactaaag atttagcgat gaagaaattt ttgatccctt gtgctttcat tactccaaaa
    11581 atagcagtct tctgaagtgt catcacgtac atgaaaatac cgaccacggc agcaaccact
    11641 aagaacaaaa agtatatcat cgcatttaag gtcatatttt gggcagaata gccaggaata
    11701 ctttcaatga atgtcccaat agttaataac tgtagaccct tcgatgcttt ttctttcgaa
    11761 attttagctg aatcctttat tacgatccca ttgatcggct gttcttcttc agaagcaaac
    11821 ggctgttgtc catacttcaa agcagtccac gtatctaggg acgcatagat gacgggagaa
    11881 actgtgtaat aagtttcagg aaagataccg acaatcgtca aaggatcgtc gtaactgcca
    11941 attttgatcg tatctccaat tttatagcct gaatcagcca gattttgaga gatgatgatt
    12001 tcattctttg ttttaaattc atgccctttc gtgagttttg gtaataaaaa tgcttctttc
    12061 gttgtgccga aaactgtaac attttctttg ttttttcctt tgattgctcc gctgtacaat
    12121 ccgattggtg ctttctctcc tccttcaatg tatttcagat ctcctcttgt taattgtgaa
    12181 gctgcaaata cttgatttgc atcctccgac aaaacaatct cttgtgcgtc ccaatcatcg
    12241 attgcttttt taaactcttc tgaaagtcca ttcgctagtc ctgacaacat gaatacgaca
    12301 tacgcaatca agaacataat accgacgatc aagccatatc gtaatttaga atagcgaatt
    12361 tctttgatag ctagaaacat ttgtttttcc tccctatgat agattgatag tgacaaaatg
    12421 tcacttgttg aatagtgtaa ctctaaagtg acaatttgtc acttgtgttg tttttttctt
    12481 atcaataaat tgtctaagac atctttcgat aaaaccttaa catgagtcat aaaaaattta
    12541 tttaagcctt atttatgctt tccatgataa actaaagtga ccaaatcaat gaggtgggaa
    12601 aatgattaaa ctattagtag tagaagacga taaagtatta tccaataata taaaagaaat
    12661 actcgctgca atcggcgaga taacacaagt ttatgatggt agtgaaggac tgttcgagtt
    12721 ggaaagtgga gtttatgatc ttgcaatctt ggatttgatg atgccggaaa tgaatggata
    12781 tgaagtgtta gcaaaaatac ggaaagaaaa aatacagact ccagtattga ttttgactgc
    12841 aaaagatagt ttggaagata agattgaagg atttgaacga ggtgcagatg attatctaac
    12901 aaaacctttc tatcgagaag aactgatcat gcgcgtgaaa gcattattga aacgctcgct
    12961 aggtctcttt gatgaaaatg ttctgaagta taaggatatc agttgtgatt taaaaagcaa
    13021 tgaagtcttg tataaagaac aagttttgcc catacaagga aaagagtttg aattgctcgt
    13081 ttatttttta caaaataagg gaatcatttt gacaaaagag cagatatttg accggatctg
    13141 gggatttgat tctgatacga cgattacagt tattgaagtt tatatgagca acttacgaaa
    13201 acatctgcga agtactggat tggataaaga aatcaaaacc ttacgtaatg tcggttatat
    13261 actgcaaagg gtgtgatgca atgaatggag atattacacg aaagcaacgt agtcgctttt
    13321 ttgcgacaaa tgtagcagca tttgctttta tcttttttct tttgggattc attactttac
    13381 agatattaaa ccaatctgca tacagagaaa cagatctgtc attagagaaa ataaattttg
    13441 attctcgaac agtacaatta gagatacaaa gatatcagca aggcgatccc tttttaaacc
    13501 cgactcaaac ggaacaaaac agaacaaaca tggaacagcc attaatggaa ccatcaaatg
    13561 accgttttaa cactcaagtg atcttgtggt ctaaaaatgg tgagatattg aataaagcag
    13621 ctattggtgg gcgtttcaac caaatcaaag aattaaaaat ggatacgaaa aatctgaata
    13681 ccatccaaaa tattgaatta aatgaagaca gtggctcatc tgccctctct ttccgttcga
    13741 ttacgaaaaa agcggagacg aatcaagatg gcgttgccta catccaaatt ttagaaaata
    13801 caaatcaaat caagaattca ttagcgactt ttcgcacgat tatgattttg tgtatgattg
    13861 ttttttggat tctctctatt ggtatcagtt attacttgtc tagtctaagc atgcgcccta
    13921 ttattctttc ttggagaaga caaaaagaat ttgttgagaa tgcttctcat gaattgcgca
    13981 caccgttaac gattattcaa aatagtttgg aacatctttt taccaagcct aatcatacta
    14041 ttattgaaga atccgaaagc atcgctcaag cattgtcaga gacgaggcgc ctcactggat
    14101 tgacttcgga tttgctgaca atcgctcgga atgattctga tcagcagctg ctttcaaagc
    14161 aaatgatcaa tacacaagaa tatatcgaac aacttgttaa accatttcaa gagatggcca
    14221 tcatagatgg aaaagagttt attctccata acgatgcaac attagaagta ccaattgatg
    14281 aaaaaaaaat ccatcaagtt ttggttattt tgctggataa tgcattaaaa tatacgaatc
    14341 cagaagataa gatcattttg gattcatctg ttaaccataa agaatggctg attcacgtaa
    14401 agaatactgg cccttctatc agtgaagaag atcaagcaag gatttttgat cgattttatc
    14461 gggaagatca atctcgggcg aaagagactg gcggatacgg gcttggcttg gcaattgcaa
    14521 aacaggtaat cgaacagcat catggacaga tttctgtatc caattatctt ccacaaggag
    14581 tcgattttaa agtccaattg ccgttgaagc ctaaaaaata atgcttatcc ctaaaaaagg
    14641 gctgtgacaa aaatcttgtc acagcccttt tttgttgcga ataaacggtg ttccagaagt
    14701 agcttcttcg gaaataagcc gaaatttctt taagctgacc acttctgtca caacctcttt
    14761 agcttacgct tacgattttt tattgcggtc ttcaatgtgt acatcaccgc ttgtgtcaat
    14821 atccaattct tctcggctaa catcttctgt cactttttca acatcttcat gttcttcttt
    14881 atgaatccca acttcttcac gaataactgt atgtttgctt acatctactt gctcttcttt
    14941 gattgggata gtaatcgtgt catcttccaa tttttcgtct gttccctgtg aagattgatc
    15001 tgttactggt ttgcgttcga tgacgatttc ttcacgtttg accggtactt ctaccgtttc
    15061 agtatcgttg accacatgtt tgtggatgtc gacttctcca gttgtcacat catgtgtatt
    15121 tacatctaat ttttcttctt ttagttgaat tgtatcatca tctagatttg gtttgcgatc
    15181 gatgtcttca gtcttgcttg tacccattgt atcagtctcg ccaggtacgt ccatcggatc
    15241 agttcctggt gtcatattca atggatccgt tgtattttga aagcctgtcg tgccacctac
    15301 gtccattgga tctgttccag tgccagtttc taatccagtc cccattgtgc ttgctgggct
    15361 aacagtatct tgtgtatcca ttgtatcttc tgttgtttct ggctgacgat agccttttac
    15421 tgtgatgaca aattttccat cagaaatatc tcctctatat tgatataaag gatcattttc
    15481 tggtgtagcc gtctcagaat cataggtatc aaaagaaaaa gtatctttta ttttttccca
    15541 taaagaacgg tcttcttcac catccatccg attttcatct gtttcaacat ctataccaga
    15601 caatccgtca aatcctagtc tagatgctgc ttcatcagta gtataaataa cgatatcatc
    15661 tcgttcatac ccttcgttac gcaaccgttc aattactgat aatgcttctt ccctcgtact
    15721 atagcttccg atgaccgtta aatttttctc ttccatgtaa attcctccta gcaaatagat
    15781 ttatgattgg tatattgtta cttataaatt tattctatag gagacgattg ttttctacaa
    15841 atatttagat tggaatttga ttaaaacaat ttaattgaaa tttgaaaaca tttatgttaa
    15901 tgaaagtcat attctaataa aatgtcattt gtttctatat gaatttttat gaaaaatttc
    15961 atataatttc cactcgttgt gatggtttaa tttatccaat ccatcaataa gcaaacaact
    16021 tatataaaaa aggattaatt ctcaatataa ttgctcctac catagaataa atcttcatgt
    16081 aaagtaatga agtaccaact atttctaata cttctttaag aattatccca acaatatcgt
    16141 tagacaaacc attattgaat aaatggagag ccaatcgatt ctgaaaaaat gttttctaat
    16201 ttttcggaaa taaaagaaag agcctcctgc cattagaacg aaccaaatcc atttcattgc
    16261 tcatgaagta accacatagc taaaaagagg ataaatcaat taaaagatca aaagaatgac
    16321 tgcactaata aaataactaa aaatgtatct tcttacttca tatcttttag ctagtactta
    16381 gttaaaaatt tgcttcctaa gatcaagaca aactgtctat cttcatttga tgaaatgcgg
    16441 taaaatatag aaaatgaaca ctattggagg gaatttgtaa tggacagcaa aactagaaaa
    16501 caactgcaag aaaagttaaa gcaacgtaca cacttgctcc cttccgtctt tcaacagttt
    16561 ttagaagaaa atcgttcttc cctttcatta ggcagtcgct atgaatacgc aaaagatttt
    16621 catctgttta ctgattattt gagaaaagta ttttctgaag aagtggatga tcagctgatt
    16681 atccatttag aaaaacaaga ttatcagaat ttcttagcta aaatctatcg acatactcgt
    16741 tcgttccaaa ctacagcgaa ccaagaaacg gagcaattgt ttgaaaatag ctattacgg
//
LOCUS       DACCKU010000066        16721 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.069, whole genome shotgun sequence.
ACCESSION   DACCKU010000066 DACCKU010000000
VERSION     DACCKU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.069"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1152
                     /locus_tag="IVN13_001994"
     CDS             <1..1152
                     /locus_tag="IVN13_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012464.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IVN13_001995"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IVN13_001995"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP7012465.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IVN13_001996"
     CDS             2728..3003
                     /locus_tag="IVN13_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP7012466.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IVN13_001997"
     CDS             3009..3776
                     /locus_tag="IVN13_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP7012467.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IVN13_001998"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IVN13_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP7012468.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IVN13_001999"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IVN13_001999"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP7012469.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IVN13_002000"
     CDS             7920..8222
                     /locus_tag="IVN13_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP7012470.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IVN13_002001"
     CDS             8225..9388
                     /locus_tag="IVN13_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP7012471.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IVN13_002002"
     CDS             9414..12842
                     /locus_tag="IVN13_002002"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP7012472.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IVN13_002003"
     CDS             12863..13987
                     /locus_tag="IVN13_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012473.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IVN13_002004"
     CDS             13977..14414
                     /locus_tag="IVN13_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP7012474.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IVN13_002005"
     CDS             14398..14736
                     /locus_tag="IVN13_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP7012475.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IVN13_002006"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IVN13_002006"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP7012476.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IVN13_002007"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IVN13_002007"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP7012477.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..>16721
                     /locus_tag="IVN13_002008"
     CDS             15893..>16721
                     /locus_tag="IVN13_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP7012478.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     "
BASE COUNT         5644 a         2897 c         3696 g         4484 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga a
//
LOCUS       DACCKU010000067        16663 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.070, whole genome shotgun sequence.
ACCESSION   DACCKU010000067 DACCKU010000000
VERSION     DACCKU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.070"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..832)
                     /locus_tag="IVN13_002009"
     CDS             complement(<1..832)
                     /locus_tag="IVN13_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012479.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(846..1644)
                     /locus_tag="IVN13_002010"
                     /pseudo
     CDS             complement(846..1644)
                     /locus_tag="IVN13_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
     gene            complement(1677..1961)
                     /locus_tag="IVN13_002011"
     CDS             complement(1677..1961)
                     /locus_tag="IVN13_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7012480.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1958..2959)
                     /locus_tag="IVN13_002012"
     CDS             complement(1958..2959)
                     /locus_tag="IVN13_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP7012481.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="IVN13_002013"
     CDS             complement(2961..3863)
                     /gene="gnd"
                     /locus_tag="IVN13_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP7012482.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            4027..4875
                     /locus_tag="IVN13_002014"
     CDS             4027..4875
                     /locus_tag="IVN13_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP7012483.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            5521..5727
                     /locus_tag="IVN13_002015"
     CDS             5521..5727
                     /locus_tag="IVN13_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP7012484.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(5929..6930)
                     /locus_tag="IVN13_002016"
     CDS             complement(5929..6930)
                     /locus_tag="IVN13_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7012485.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(6935..8848)
                     /locus_tag="IVN13_002017"
     CDS             complement(6935..8848)
                     /locus_tag="IVN13_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7012486.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(9016..9522)
                     /locus_tag="IVN13_002018"
     CDS             complement(9016..9522)
                     /locus_tag="IVN13_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7012487.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYFIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            9682..10122
                     /locus_tag="IVN13_002019"
     CDS             9682..10122
                     /locus_tag="IVN13_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7012488.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(10148..11305)
                     /locus_tag="IVN13_002020"
     CDS             complement(10148..11305)
                     /locus_tag="IVN13_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012489.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(11308..11664)
                     /locus_tag="IVN13_002021"
     CDS             complement(11308..11664)
                     /locus_tag="IVN13_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012490.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(11962..12855)
                     /gene="sgrA"
                     /locus_tag="IVN13_002022"
     CDS             complement(11962..12855)
                     /gene="sgrA"
                     /locus_tag="IVN13_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP7012491.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTGKVNNELDLVPII
                     PTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWM
                     GVLLILIVGASYFRSLFHRVK"
     gene            complement(13051..13890)
                     /locus_tag="IVN13_002023"
     CDS             complement(13051..13890)
                     /locus_tag="IVN13_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP7012492.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            14075..14962
                     /locus_tag="IVN13_002024"
     CDS             14075..14962
                     /locus_tag="IVN13_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP7012493.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(15546..16241)
                     /locus_tag="IVN13_002025"
     CDS             complement(15546..16241)
                     /locus_tag="IVN13_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP7012494.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGAYYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(16225..16623)
                     /locus_tag="IVN13_002026"
     CDS             complement(16225..16623)
                     /locus_tag="IVN13_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7012495.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         4992 a         3383 c         2565 g         5723 t
ORIGIN      
        1 aaaatccgtc atcatagtca tcttcgtcat caaaagatgc tgaattgtta ttgcttgtgg
       61 caggctgtac ttgtttccct ttttgcgact ggacaaagat caaagcgata ataatcccaa
      121 aaatggcata agtaaccatc gtaatatcta aagatttcaa aacaacactc ataaaataag
      181 caaggaagaa aaagaccaga taatcttttc gaccgataac gtatacagtt gtcgcgatac
      241 cgattgccgc taaccctcca cctactactt caagaatgtg gataacgact gttccctcta
      301 aagctttgat tacatcagcg atcaatggcg cccctaaata taaagcaatg aaaacaagcg
      361 gaacgaataa taaaaatgag gcaaccgttg gataaagaat aattgatcgg gccatagctt
      421 ttgtattcaa atcttctaca gctttgtctg tatatttacc taaaaaagta ttgatgaaaa
      481 aacgaaactg atacaagtaa cttccgagca atccgactgg caccgcaacg gcaatcgctg
      541 cttctggcga cagatcccct aataatgcga ctggtactgc gatagctgct gcaatagatg
      601 gttcagctgg cattgatcct cctggagaaa aaacccccat ataaatcatt tgcaaagcgg
      661 ctgtaacaat catcgcttgt gcaacttccc ccatgatcaa cccgacgact aatccagtca
      721 tcaaaggaga aaaacgtaag gtcaacgtgg ctcctccacc taaccagcgg gacataaccg
      781 cggctaccca taaagcaatc aacaaacttt gtactacact taatgtttcc atgtgtcctc
      841 ctccttcaga tctttctgat ataaccttcc agttccatca ttgactgtat cagtgatttc
      901 tgtgttacct ctataggaat caaatgcact ttctctttcg aatcaataaa ttcagccatt
      961 ttatctatta tctgtttatc tttcggaaat aatcccattt gttctagaga aaccggcagt
     1021 tctaattccc gatacaaagg atataactcg tctatttttt cccagcgacc ttctagtgcc
     1081 agttgataga aaatcccata cgctactttt tctccatgta gaaaacgatg gctttccgga
     1141 atatatttac tgatagcgtc atgcatagca tgtgctgccg catttcttgc gtatttatct
     1201 cctaatcctc caactaaacc agcaacagca aagataatct ctgaaagctg tttgaactcc
     1261 gatgtcagtt ttctttgtgc catatcttgg attgctttag cagaatgatt caaaatctct
     1321 tctttgcaga tcttcgcagt atcttgtgct agcttcaaaa atgattcatc ttgcaaaaaa
     1381 tcttgttcta agatgagctc agattcatac cattttgcta gtgtatctgc taacccggca
     1441 atgaaatatg gaacagggga atcaatcact aattggggat cctgttataa aaaaagcagc
     1501 ttgtctgtaa aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc
     1561 aagaggtgtc catggagcac aattactagc taaagtaggg atgactccga aatcgattct
     1621 tgcaagattg gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac
     1681 gaaatcgatt tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat
     1741 tctccagaat aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta
     1801 ggtagcgcct tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat
     1861 gatttcaaga gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg
     1921 cctgttttca cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct
     1981 ttgtctggac agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac
     2041 atttttcacc cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca
     2101 ctacttgttc aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat
     2161 ctgtggcata tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa
     2221 ctagagcgcc tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt
     2281 tatcagaaat catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg
     2341 attgttccag taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat
     2401 ctgatttgta aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga
     2461 ttactccgct tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc
     2521 taccgacgaa ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg
     2581 tcgtgcgtcg aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt
     2641 caactaaagc aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa
     2701 tattgttgta accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa
     2761 aatctcgtcg aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact
     2821 ccgctagttc ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg
     2881 actttaatct ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg
     2941 ctatatgata atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca
     3001 tttcttagtg ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc
     3061 atgaacaaac tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc
     3121 gtccattttg cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt
     3181 tcgtttaact gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc
     3241 caaacacgtg cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat
     3301 ccttcaccga ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag
     3361 tgtccgcttc cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat
     3421 aattgttcca cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt
     3481 cgtgctcctt cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga
     3541 aactcttctg ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg
     3601 tctcctggtc cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca
     3661 ggaagcatta accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct
     3721 tgataagcag gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt
     3781 ccttgtactt tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc
     3841 aaaccaatta caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa
     3901 aaaatttttt tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc
     3961 cttgtatctt taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga
     4021 attctcatga gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca
     4081 aaagaacagg ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct
     4141 attagcaatt tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa
     4201 aaacttggtt ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat
     4261 ctatctgcta tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg
     4321 caaaaggtgt tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa
     4381 aaggatctaa aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt
     4441 gttggcggtt cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc
     4501 gcaacttctc acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa
     4561 aaagactgtg ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct
     4621 gaaaccgtaa gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc
     4681 cttgctaaac ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct
     4741 gaagctttag cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg
     4801 atgttttaca acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct
     4861 gaaatcaaag aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca
     4921 ctaccaaata tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag
     4981 tcggtgggtt agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga
     5041 gttacttgtt agatgcgata acaaagttag tatacagtag taaaaaattt atcttttctt
     5101 ttttttatta tttttgtttc ttattttcta aaaaattaaa aaaaattttg gtgaattaag
     5161 tatttccttg ataacactgg cttttggcct cattttaggt tatcatttgt aattttattt
     5221 cacagttgat atgattgtta tgtaaaataa aaaagtttag gatacatcga atttttgggg
     5281 aaattctttt gggggtatcc taaatctttt ctattatatt cccatatttt ctattattgg
     5341 tctagacgta tgatggtaaa ctttgtacct atctgattgc tgaatcatta attgcttatc
     5401 attagccatc tttgtttcaa gttcgctaga tgatcatttt attcacatcc taacaaactt
     5461 gtcacattta tagattctta atctatttat catacaatta taaaaaagga ggtgctgctt
     5521 atgaaagcta tccatatttc tttgcttttt ttcgctagtg cattgatttt atttgctatt
     5581 catctttgtt cccagcctta tatcaataac aacggaatat tagtggaacc agcattcttt
     5641 tgcttaccac tcggcttttt gtcccttctt gcctccgcag gcttcggatt tgttgctttt
     5701 tttaaacgat caaaacaaca aatctgactt ataaaattta cgtaaaagag atcgtgacaa
     5761 taatcgtgtg atattcccaa atttcgaata aaaggtgccc cttgccatct ctaactcctc
     5821 agtcacaagt tacaatattc ataaaccatg aaaagcttgt ttattgaaat tgctccttgt
     5881 gacttggagc taagtgacag attacaacct cttttgcatc ataagacttt actccttgaa
     5941 aaacattact aacttttgat atgtttcttg cgcttgttta ttgctgaaat caaactgata
     6001 ttcatggttc aaaacagcat ttttctccgc ataatctact tttgtgactt tcacccccgc
     6061 tttctcaaat acagaagtta cttcttccat ttgtttcgta aacgtatttg tatttccatc
     6121 cgttaagaat gtgttttgcg tccaacgatc cgcatgtctg accaaggcta aacttttgac
     6181 cttctctgat tgttcaaagt ccttattatc aaagtaagcc catccacatt tttcataaag
     6241 aaagctagag attttatcac ttacttgagt aagtgcagaa ggatccaaca gaccagacaa
     6301 agaaataaac ttcgtaatct gcttgttctc caaagtcgga tcaagatgat cagctttttt
     6361 aatttcttca ttttgaagtg ctaacaaata ttctccacta atttgtgcac ctgccgaatt
     6421 tccaccaatc gaaatattcg accaatctgc ttgatctttt atctcttttt tagctgcttg
     6481 aatggcttcg ttcaattgga taactggtgt aggatgtttt gcttccggtg atcgttcata
     6541 atctatattt acgatgattt gctgcgtgtc tgaacataga tattccaaat aatcacgaca
     6601 atcattttta tcccctccta catatgcgcc tccatgcatc cagaaaagaa tcggtaattt
     6661 tttcttttcc actttcggtg tgtagatatc catattatta tttttgtact ttgaaggata
     6721 ggatatttct ttttttatat cgatttgttc gattttttgc tggaaatcat ctggtcgggc
     6781 atatacttgt tctttttcac ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt
     6841 tggtgtgatt tgattaatca aaaatacagt gactaaaata actacgatga taactggtac
     6901 tgcaatcact attcgcttga accatttcat tcatttaccc tctctttctt cgccaaatcc
     6961 atgtgataat cgtgctaaca gataaaaatc cacctgaata aatgagaagc gaatgtttct
     7021 tttctccagt tttcggcaac attctttttt ttgcatctgt ctttttttgt actttcactg
     7081 tcatctgaat cggttgcgta tcatcacttg attgatcctc tcgaggttca ttctttccct
     7141 tattgtctgc attctctgtc ggatcttgct tttttggtgg agctactggg atttttttgt
     7201 aatagaaaat aatagttggt atcgtctcat taaaaatccc ttcagtcggt ccttcaacat
     7261 ttgctaattc gtatccttcg aatgtctttt gctctgtcat ataattttct ccataagctc
     7321 cgtaaagctg gatttcgtcc tggtctttga gtagactttt attgttttca tccacatact
     7381 ttacgtttat ctgatcatgg aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg
     7441 cagcatggtc aacttcctgt gcctttccat gccctgctcc ttctactaat aaaagatctt
     7501 tataccctcc tgataactca tataattttt gcgcgttaga cacaggtact acatcatctg
     7561 ctgttccatg gataatcaat ttaggcaatg aatctgacat tccaatcgta ggtagagcag
     7621 cttttgtatc caaatgcatc tcctgatcaa aatagtattg tttcaaaagg cccataaata
     7681 aatcttcatg ctcttgatcc ccaatgatgt ctaactgatt ttttatggca tccggtagtg
     7741 cgggggctac tgtctggctg tacgtaaaac gtagttgttc atcaattgat gcaaatccgc
     7801 aattttcaat aattcctttg acattgacag gaatatcttt cgctaataca gacatcatcg
     7861 tagctgctcc catcgaacca ccataaagta aaatcttttg agatggccgc tctcgaactt
     7921 cttgatttat ccagtaaagg acgtcatctg actcatattg tccataggtc acataatcgc
     7981 ctccgctatt accagtcgct ctggaatcaa caaataataa attatacccg gccttataaa
     8041 aatcattgta ttcttctgta acgatcccgt tattccaatt ccctcgaaat ccgccatgga
     8101 tcatgactgt tttatctgaa ccttgatcga cataatatgc atatacatcc atcattttgc
     8161 catcttgttt gtttagtaca gtttgatgaa gttgttttgt atttggatag aagcctgtgt
     8221 tttctgtagg cgttgtccca tcatctgtac ctccaagcat cgacatcaac acactgtttt
     8281 ttgcaaaagc gatatcgtac aatagataag tagcaatcag atgtttgata atctctttat
     8341 ctacaccata atcattaaca gcaagctcag tgactaataa ctctattaat tttgacttga
     8401 tgtcttctgt tggtccatta agtatttctg tcatctgttt accgaagtaa ttcaaatatt
     8461 ctttaccatt gaaattttca cctttaccga tatcatcatt ttctgtccca ttctctttgt
     8521 cacttacttt agcagaagag gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg
     8581 tttgttggtt atctgtggta gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa
     8641 ccgttgtttc agtatttgtt tctgtttgtg aagtactcga tttcgtttca tttccagcat
     8701 cagtagtttc tgtggtttct acctcccgag attccgattc ggaagaaatg ctctctccat
     8761 atactgtcca ttgcgggacg cttgtactta cagcaaataa gatgaccaac gctcccttta
     8821 atacttgttt tgttctcaag ctattcactc ctttttatgt ttctaggaga attatatgta
     8881 aacgcaaaca ttttttatac aaaaaataaa tggtttatcc cataaaaata gaacaggatt
     8941 attttctcat tttacgtaat aaagcagaat ggataaacca gtatccattc tgcttttttt
     9001 aaacgaagaa acaacttatt gctccaaaaa tgtcagaaat cgtttatccc aaattttctc
     9061 gtaaaaagcg attgtttcct ttgctttcat aaaaggatta tcataagttg tcgttgctcc
     9121 tctacacatc ataagctggt atcctaatcc atgagcaaac ttcacagtcg tatctataaa
     9181 atactctgct tgtgccccac ataattcgat cgaatgacag tccaattcag ttaataaaac
     9241 actcaaatta gtcttataaa atgaattcgc atgggttttt ctcacaaaat aactgtgctc
     9301 aggaatagat aattccgaca atagctccca tggttttgtt tgtggcacta attgagcatc
     9361 ttcgtgttga acgaaaataa ctggtttccc aatcgtttcg tatgttttga ttcttttatt
     9421 cactaatgct gtcactgttt ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt
     9481 ttgtaaatcg atcactacta ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt
     9541 ttttcttgat ttaatagtat ttcttatcta gctagttcca ttatacgtga caacattttc
     9601 ttattttaca cctctaaatg gcacttttcg actatttctt catgttatta tttcataaat
     9661 acttagctat tggaggaatg tatgatgatt aaattgtata caggttctgg ctgctcgtct
     9721 tcaagacgcg caaaacgttg gcttatcgtc catgatatac cgtttatcga acagaactta
     9781 attcgtcaac cactaaccaa aaatgaattt atccacttac taaaattgac aaacaacggc
     9841 gtgaccgaaa tgctctctac tcattctatt agttataaaa atcttgttca cagaatagat
     9901 gaactcagtt tgagtgaact atatattgag attcgtacat ccccttcttt attgaaaaaa
     9961 ccgattatct acgaccaatc tgcaaaactt ttgactggat ttagcgaaga taaaatcagg
    10021 atgttccttt ctaaaactaa aagaaagcaa gagctcaacg aactcttgct ttctggtcat
    10081 cttgtcgatt ttaaagaaaa taatcttttt gcaatgtctt aattttggca taaacaatct
    10141 gcttttctta attaacgaaa tccaattttt ccaatatgga tctatttttc tcattcctga
    10201 tttttatgat ttcagctttc agacgcgccc aatcattttt tgtaggttgt ccattccagt
    10261 aaaatatctg tgtattcgtt tgatcatatg cctttttgtc gataggataa tcgctaatga
    10321 caatatcagg taactgatca gcggatgcac tatattttat tgggcgttca gtcatagata
    10381 atatcctttt ctttgcccat aattcctgtg ttttaccata ctttgattga atatagaata
    10441 tgacaggttg ttcgtgtttt ataaaaatat ccctgactaa catacagtat tgaaaaatca
    10501 accgtctgtt cttttcataa taagtattaa gctctggaat tctgcgagct accccactaa
    10561 aaaactgttg catgattgga tacatcactg gaaatatatg ctgaaaatct ctgtcttctg
    10621 ctgattttcc aaaagcatcc accttctttt ttgtcccaaa aacccttgaa tacaaatgga
    10681 tcattgccaa attgacatat agataatttt tttctgaagt tgtaaaacaa atcttgaagt
    10741 ttttttcaaa attttctaca atagacgatt caatccagcc ccaactttct tgtactttat
    10801 ctggagtatc caacaaatct tcttccaaat aggtgttcat tacaccaaac ataaaataaa
    10861 ggtaagcttg ctctgttgga gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag
    10921 gctctagtat gtaatgcgta aactcttctt ttgttatcaa tggatgcaca aatttgttca
    10981 tctcttctgg gacatacgta atcgagtggc cttgtttcat tcgttgcaac gaaattctta
    11041 gtaggctgat ccatttcttt ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt
    11101 tttctaatag ttgacgttta ttttttatac ttgataatat atcagaatga ttttcgtcga
    11161 atgactgcca atacatcaag tgaaaaaagt atcggatacg gtattcttct ccttctatcg
    11221 gattaccttt tttcaagctg caagataaat gatagttttt caaaatagta ttaagttctc
    11281 ttaattttct ttgaatcgta cgcaaactta tataattttt tgactaagat tttctaagga
    11341 ttgataagta cctttgtaaa gtgaatccag taaaatataa ccaatcgttc cctctaaaag
    11401 tatagaataa actttactca gtaaaaactg agaagaaaac tcgactttta tcatcttgtt
    11461 ttctgaaaga aaagataatt tatattctgg atacctttcg caaaacgatt ctgcttgttg
    11521 aatcaccgaa ttcatcttag ccagggtgta gtttagatct aaagcgatat ccttcaccac
    11581 atataatttc tcgtctcttt cataacaata gttcaaaaat tgatatactt gcctttcatt
    11641 gatatcaata aattcttcaa acaaagtaca tactcctttt tatctattat ttagacttat
    11701 tccgacagct acatgaataa cgtacaaata gattcgttac tgatttttca gagatactac
    11761 catgcgcagt gtgcattgtc aattattctt gctcttattt tccatttgtg ttcctgttat
    11821 ttcctgtttc tcaaatatgt tagtaaacaa aaatagatct ggcaaagcta caacttcgcc
    11881 aggcaataca gcactctcga atgaaatatt cactcaaaaa tctatcgttt tattcaattt
    11941 tagatctgtt gcttttattc tttatttcac tcgatggaag agagaacgaa aatagcttgc
    12001 tcctacaatc aaaatgagta acacacccat ccatgtaatt agcttgttca aggttctact
    12061 accagtttct ggtaaatcgc tttttctatt atctgtttct ttcgcttttt cttgatgatc
    12121 aattccacga gaaacaaaat caggctgttt tagcacactt tttaggttgc tccctgcatt
    12181 tctcttttgt tccttagttg gtatgatagg taccaaatca agttcattat ttacttttcc
    12241 tgtgctgctt tcgctgctcg tgcttcctgt gctactttca ctactcgtac tctccgtact
    12301 gctttcgctg ctcgtgtttt ctgtgctact ttcactgctc gtactctctg tactgctttc
    12361 accgctcgtg ttttctgtac tgctttcact acttgtgcta tccgtactgc ttggcggtcc
    12421 aacaactgtc caactgcttg gtttcaaatt ctccgtgatc gtcatctgtc catcataaaa
    12481 aacaatcaaa tcaactttat ctttactctt gatctcatca atcgatagtt ctgcagaaat
    12541 cattattgta tactcaatac cagcggtcca ttgtacgtct tttaaataaa cttccccaat
    12601 aactttttct gaacttccat taccatccca accatatgtt agaacagcat gtacatcact
    12661 agcattcacg cttttgtttg gtttgactgg aatagtaatg acttgttgtg tcatgtcata
    12721 aacaatccga tcagttttca cagtaaatga aagattctca tttgcccgtt catttgcaga
    12781 agtactgatt ccaatcattg ctgcaattat actcagccca agaataacaa ataatacggt
    12841 cgctgttttc ttcatttcta tcactctcca tataattacc ttctatttga taaaggaatt
    12901 atataaaatg aaaatgatga gaacacgaca gtcagttgtg aaaatctaac aaaagatgtc
    12961 gtgtattcgc ttgaatttgt ttactttttc gtttaatcaa tgacagactt aaaaaagcaa
    13021 gaccgatatc agccttgctt tcttcatctt ttattgataa acagcagtgt atttcttcat
    13081 ccgctttgtt ttatatgcat agagtaacaa accaacaatc aagactaaca agaataatgt
    13141 agttgatata gatgcttcag ccccaaagtt tcccccagta attattgtat tttgcgattg
    13201 gatcagagtg gagaaaaaac cttgttcttg cgttccgctg actggtatac ccaaaatagc
    13261 tccttgtgtc caattccaag taatatgagc accaatagcc gtaaataaac ttcctgtact
    13321 gtagataagt gcgctgaaaa agcaacctgc taaaaagata ttgaaaactg ccagaaatgt
    13381 catgttagga ttcaatccgt gcaaaagagc aaataatgca gcagatccaa tcattgctat
    13441 taacggacca aatctaagca tctgatgctg aaaaaagcca cggaataaaa gctcttctga
    13501 tgcccccact gcaataaata aaataaaact cgttatcagc ggcacaatgt ttgcgtttcc
    13561 tgaatctaac cgatacgccc ccgtcaaata taatggacta gtaataagca gaaacactcc
    13621 aattccaaca acgagacctc ccgctaaata cccccagtct tttcctcgaa cagaaacacc
    13681 ataatcttct attctttttc cttcgacctt actagcaaga aataaagcta gtaaaaaaac
    13741 agctaaatgt ccgaccgtac cggcaattgc tccaatccat tcactatttc caataaacat
    13801 atccgcaagt gcattattga caagaattcc taaaacaaaa ccaactaaga ctattaaaac
    13861 agatccaatc aaccgaataa tcctgatcat ctttcatccc tcctttatat taagtttaac
    13921 aacccttttc tttcttgtct cgtgaaaact atgtaaatgt tctccatcat agagagaata
    13981 gatttttacg aactaagaat atctacagca accttacgaa aatgaaagac tgtctcccta
    14041 attcttctgt tagactgttt ttaataagga aaggatggga aattttttga aaaaacaact
    14101 actaattatg tgcctttcag catgtgcaat tttactattc tcaggttgta gcaagtccaa
    14161 tgaaacgatt gcaacgttta aaggtggaac acttacagtt caggattttt atgataaccc
    14221 aaaagttaaa tcaaataatc aaacactatt aaaaaaaatg attgtcttca aagcatttga
    14281 tgatgtatac ggaaaagaga tttcttcaaa ggaagtaaca aaagaatata atgaacaaat
    14341 caaaaaactc ggtccaaact acaaggaaca attaaaagca gttggccaaa cagaagagac
    14401 atataagaaa ctctttaaac agatgctggc tttccaatac ggtttgaaag ccaatgtaaa
    14461 attaacagat aaagatctag acactgcttg gaaagaattc tatccagaag ttagtacaca
    14521 gatcattcta ttttcgacta aagaagaagc agataaagca aaaaaagaag caaatgaagg
    14581 ggaaaatttc tccaagcttg ttcaagctta tggaaaaaat aaaacattaa aggaaactga
    14641 tggaaaaatg aattttgatt caaccaatcc agagattcct actgaagtaa aaaaagcagc
    14701 atttaaacta aaaaacgggg aagtttctga tatcattcct gtaacagatc caacgactta
    14761 tcagcaaagc tattatctag taaaaatggt aaaaaaacag gataaaggtt ctaataaaga
    14821 caaatacaaa tctgagctag aaaaaattgc taccgaagca aaaactcttg atacggaatt
    14881 catggataaa acaatccgta aagtgatgaa aaaagacaat gtaactatta aagatcctta
    14941 tgtaaaaaat atattcaaat aaacaaattt gacccaattg gggatgtggc aaaatttgag
    15001 aagtgatttt tggaaattcc ttcttatttt tgaggcaaac cacttttgcc acaacttcat
    15061 cggggccttt ttttatttgt accaaaacaa atcactggat ttgttacaat catgaggata
    15121 taatttaaaa aagtttttta tttctatcac atgaatcact gcgccattaa gtagattctt
    15181 tcatgcttcc ctattttttc acttgccgta tcgcttcaaa aacaactatc ataaactcaa
    15241 actcgactga aaagttattt taaaaaagac taaaaatatc ccttctggac ttgttcttct
    15301 tattctgtta tactagagaa acacgaaaga tatttttatg cgacactttt catttgtttt
    15361 tatgaaattt attcaaaact ggcgtaactt cagagttaca aggatgtatt agaggttagg
    15421 gaatacatcg tttaaatgag aaataagtaa tagtcaccca aactttaaat taatgaatat
    15481 tgaatcgtca gatagcctcc tcgagttttc gagaaggcta tcgtttattt tatggagaaa
    15541 ttgattcaga agtaatcagc gacctttttt agttcaccaa tcgtttctgc ttggaaagtt
    15601 tgcacccaat cttcttggaa gcccctcact gctttatcaa tcgagggcat ctctaatact
    15661 tgatctagta gctctttacc taacgttatc gcatgagctc cagatcgaat ggctgtagtg
    15721 acttgttcac aatttttgaa actagctgct aatatttttg ttggtctttg cgtacgttcg
    15781 atctcagtcc gcaacgaatc aatcactttc atagcatcaa tgccttgatt ttgcatcctg
    15841 ttataataag gtgcaatata atatgctcca ttattaatag ctaaataccc ttgtaattct
    15901 gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt tcaacttttt gatcgcttga
    15961 aggcctgctt gattcaccgg caccttgata aacgtatctt ttcccagttc ttctatgata
    16021 actcgagcat cctctatcat ttcttcttcc gttcttccta ttgtctgaat atggatagga
    16081 atctgtccga ttattttttt gatttctttc atatgcgaga agaagtttga cgctccttct
    16141 tttttcagaa ttgtaggatt tgttgttacg cctgttaaat tcaaaaactg ttgacaagaa
    16201 taaatacttt ttgtatttgc tgtatcaagc atgatttcca tatagctttc ccccattctg
    16261 atcctcttga tcgattttaa tgattgtccc ctttcttaaa tgcggcattt ccttatcttc
    16321 tacatacatt gttcctggaa gttccggctc atgagatcca tcaaatttta tcgtgatatg
    16381 tcctaattca ttcaaattag tttggacaac atctcctact gctgtgatta catacatttg
    16441 tttatcaaaa cagagcttca tatcacaagt tatttctttt cttacctgat tcaattcaat
    16501 cttataacaa taatctgtta atgtttccgg tgcttcatct ccaaataaaa tcattatttt
    16561 ttctttcctg aatacttctg cttctatacc aacatctgtt acaaccgttt caaagatcgt
    16621 catctctgtt cctcctcgta aaatatcatt tgtctaatag ttt
//
LOCUS       DACCKU010000068        16535 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.071, whole genome shotgun sequence.
ACCESSION   DACCKU010000068 DACCKU010000000
VERSION     DACCKU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.071"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..417)
                     /locus_tag="IVN13_002027"
     CDS             complement(<1..417)
                     /locus_tag="IVN13_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012496.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQSLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGIS"
     gene            404..2041
                     /locus_tag="IVN13_002028"
     CDS             404..2041
                     /locus_tag="IVN13_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7012497.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLGYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATALTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPENAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(2186..2926)
                     /gene="truA"
                     /locus_tag="IVN13_002029"
     CDS             complement(2186..2926)
                     /gene="truA"
                     /locus_tag="IVN13_002029"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP7012498.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEVPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(3117..3752)
                     /locus_tag="IVN13_002030"
     CDS             complement(3117..3752)
                     /locus_tag="IVN13_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7012499.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(3899..5140)
                     /gene="sstT"
                     /locus_tag="IVN13_002031"
     CDS             complement(3899..5140)
                     /gene="sstT"
                     /locus_tag="IVN13_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP7012500.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            6349..8367
                     /locus_tag="IVN13_002032"
     CDS             6349..8367
                     /locus_tag="IVN13_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7012501.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVGAFFYCFIFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKANEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYSNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8427..9137)
                     /locus_tag="IVN13_002033"
     CDS             complement(8427..9137)
                     /locus_tag="IVN13_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP7012502.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATIATTTTTS
                     TESKPTAASTETADTSTSSESSKATETTQTSTSSSSVEASSSESSEQVEQILWNADKS
                     AQLEAFMATWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEWSE
                     SGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNET
                     ANNELKNGFNQIVYGQAN"
     gene            complement(9364..9906)
                     /locus_tag="IVN13_002034"
     CDS             complement(9364..9906)
                     /locus_tag="IVN13_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7012503.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIDNEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNMFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9911..10138)
                     /locus_tag="IVN13_002035"
     CDS             complement(9911..10138)
                     /locus_tag="IVN13_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP7012504.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(10217..12097)
                     /locus_tag="IVN13_002036"
     CDS             complement(10217..12097)
                     /locus_tag="IVN13_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP7012505.1"
                     /translation="MKFQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYMTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLVALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFAKIDTFVFDKTGTLTKGTTAVSTIKNYTENE
                     GDSLALVARLETLSDHPLGRAVIHYVQEKDIDFQQLAVKDNQTIKGQGITAEIDGHAV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSFVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(12287..12901)
                     /locus_tag="IVN13_002037"
     CDS             complement(12287..12901)
                     /locus_tag="IVN13_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012506.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMGNELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKIKGIGTQLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            13043..13603
                     /locus_tag="IVN13_002038"
     CDS             13043..13603
                     /locus_tag="IVN13_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7012507.1"
                     /translation="MKKTDRRVKKTEKALAEALSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13908..14222)
                     /locus_tag="IVN13_002039"
                     /pseudo
     CDS             complement(13908..14222)
                     /locus_tag="IVN13_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14476..16116)
                     /locus_tag="IVN13_002040"
     CDS             complement(14476..16116)
                     /locus_tag="IVN13_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012508.1"
                     /translation="MQVKKKIILSILLAIIAGLYSINYFRNVHTISTSGDIAFHFARV
                     KGLSSIFSGPINFTTFNHYGSGVNYFYPYLTFFPAVIFYWISNNLIVSYILYVWLLNV
                     CTIMLMFHYGLKFLKRIDAAFIFSCLYTFYGYRTIDIYHRSAIAEAIALTVIPIVMYY
                     AYALIYEKKPCAIPLAVSMSLLIYTHVLSTFMSVIAVGLLVLGGFVVSETRKKESIRL
                     IVELSKAVGMTILLTAYFWYPMFRQIMVQSINEPDKTTLQHQALNVSDSLIGAMNNDL
                     TTFTVGIIGIISLICPLFFFKRFNRKEKVIYLSAVVSWLLSTKFFPWSLLQNTPIQMI
                     QFPWRILGFQVLFGSLILSFVFMKSTSAKQRKVVNITTIVLLLLAVSAATKANYEHKV
                     VTQRDHLVMNQENLIRYTTLLPGGLYDYAPTEALQYKEHLQNHEVRIGKEWHTMPYQV
                     MDSKIVYTVHESKGQKITLPVFDYWGTVAKVNGQKREIVNSEGLVSIEGTQGETIIEV
                     SSIYPPTMKLAFLISLCSYLFLLISFLIRKLQHIKIMI"
BASE COUNT         5254 a         3144 c         2579 g         5558 t
ORIGIN      
        1 tgaaattcca taaaaaacga ctgcaggaat tattgtaaga aaaggataaa aatagttcag
       61 tccatctcca taattgttaa atgttgtata gttgattggc ccttcaaaaa tagaagataa
      121 ccctttgatt cgtgaaagat gaaaagataa atcacctgtt gtataaattt cttgggcatt
      181 tctcataaaa tttactgaat ataaagtact aagtaagata agaactacta aaagaaatgc
      241 ttttttactt attttcatag atttgttgtt ctcctttttt aattctattt tcagtatact
      301 agaattgtct ttttttaata agctttgcgt taaatttttc atagcactag tatgatatac
      361 tagaaaagaa tcaattttta ttacttttaa ataggagaaa aaaatgaaaa aaaacaaaaa
      421 ccaaataata gatatcattt ttatgttatt tctaagtttt gcttacgtca tccctctatt
      481 catgtctaag ggaatctatc atagtgtcaa tcaggatact tactttcatt tatctcgaat
      541 cataggacta gataatgtat ggagttctcc agtaaacttc aataacttcg atcatcatgg
      601 aacaatgatg aatatttttt atccatggct tactctttat ccagcttttt tattttataa
      661 aatgatggga aatcttgttt taggttataa tatctactat ttttttatca cttttttaac
      721 aatggtcgtt tcttattttt ctatgaaaca aataaaaaac aatcgttata tttcgctgtt
      781 attttcgatt atttatacat tttccgccta tcgtgccatt gacatattcc atcgtgcttc
      841 attgggagaa gctgtcgctt taacattttt gcctttgata ctaatggggt gttacgaaat
      901 ttatattaga gattaccaaa aatggtactg gttgtctatt ggcatgacgc tagtagtgta
      961 tactcattta ctttctgtcg ctatggtttc tgtatttatt ggaggaacgt tgtttctttc
     1021 tttttatttt tgggaccaaa aaatcgctag actcttaagt cttttaaaag ctaccgccct
     1081 cacctttttt ttaagtgcgg gattcttaat tccttttatt caacaatcta gagcacaaga
     1141 attgaaagtc ccactgggaa aagaattaag tggtatggct cctagtgata tgctgacaca
     1201 tattttaaat aataactata acaactatac tattggatta ttcttatttc tcggattgat
     1261 tggtgctttt atttttataa aaaaattaac tgctgatgat ttatttattt tctttcttgg
     1321 tgtatttgtt cttttttgtt caaccaattt atttccttgg caacttttta accatacacc
     1381 agttaagagt ctacaatttg tctggcggtt aaatggtttt tcttctttat ttatagccta
     1441 tacgatgagt atagtgatct attatatttt ttctactaaa aataattcat ggaaaataat
     1501 ggtctttacg tttcttgcac taatattgca tctctcagga gtaagcaatt ctatccatgc
     1561 taacaaagat tcattaacac ttatagcacc tgaaaacgct gttgctatag ctgaaaatta
     1621 caatcatact gattatgcaa acaaagagtc aatctatcat cctgatatga ttaataatga
     1681 tcaatattta ctaggtaacc aggaaataaa tccaacaact cattttatga gtaacaaact
     1741 tacaattgaa ttagataatt cgaataataa aaatactgtc ttgacgacac ctatttatcg
     1801 ttataaaggg caagtggctt ctattaatgg caaactagta caaacaaaat tatctaagtt
     1861 tggcacaact gaattaacta ttcctccagg tataaataaa gtcgttatta cttatcagta
     1921 cacaaaacta gcaattgctt caagatattt atctatcgtt actttgattt tatttttact
     1981 ttatcgattt actttttcta aacaaaaaca accgagaaga gaagtacaac attctcacta
     2041 gatttttatt taagaaagac tttttagctg tacacataaa caggctaaaa agtctttttt
     2101 tatatttctt taatttgctt catacattat ttaacatcga tatttatact gtgaaacatg
     2161 cgtttcatcc attatcaaag tatttttatc gataataaac ttcatccaaa aacagacctt
     2221 gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt tcatcgattg
     2281 tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata atccgcacca
     2341 ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc tttcgttcga
     2401 tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat gcagaaaaac
     2461 caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg tccatatcca
     2521 actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc acgttgttcc
     2581 aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag cgctcaggca
     2641 cttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata aagtcgatca
     2701 tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct cgcgcatggg
     2761 tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc atctgatgca
     2821 atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt tgccatcctg
     2881 aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt tagtggctcc
     2941 tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa atatagctcc
     3001 ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt tcagaaaaac
     3061 aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct caagttttac
     3121 tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa aaaactgatt
     3181 ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg aattcttgcc
     3241 agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta taagtggctt
     3301 acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa caataaaaaa
     3361 ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt ccacgaagat
     3421 tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc atagaacgcg
     3481 atggctgctg taactagggt agctaataca cgagatgtac ttacaaatgc agcatacaat
     3541 gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag aaacgctttt
     3601 cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg atctaattca
     3661 ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc taataataaa
     3721 aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca attacctatg
     3781 cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct atttacacga
     3841 acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata tctaacgttc
     3901 aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca cttcttttac
     3961 gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt aaggcagttt
     4021 ccattgaatc ctggataaca ccgataataa atccaatacc aacgatttgc atcgcgacat
     4081 cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa ccgcccgcaa
     4141 cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact ttcaaaacaa
     4201 atggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa atggtaatcg
     4261 cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa gactcttttg
     4321 tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca gaacttcggg
     4381 taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga tagggatttt
     4441 ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac agcatcgtac
     4501 cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca gtcgtagcaa
     4561 ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc acttgtacga
     4621 cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcgattct gaacgtgctc
     4681 gaaaagctag tcccaacaat acagcccaga ataaaacgcc aaggtaattc gcatcaataa
     4741 gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca ctttccaaac
     4801 tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta aaaattgaac
     4861 ttacaataac tgctacaagt gcagaaagca atgttcccac taaatataag atcaaaatag
     4921 gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc gtcaagaaaa
     4981 aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgttccc aatattgaaa
     5041 taaatgtcca tgtggggaaa aagataccaa acaatattcc gactaaaata ccaacaacaa
     5101 tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc tttcacaatt
     5161 ttaacaatca ttgtatcatt ttatgcaaag aaactaaagg tataattttc taaaaattct
     5221 aacaatacaa acaaccattt cttgctaatt ttgtactttt tctgtctaca cgttgttttt
     5281 tgagatattc taagttagga tgttattaat gggcaactta tcaggaaacg ttttcttatg
     5341 tttttgtcca atttttttgt gaggtgttta ttcttgaaaa aaataaagaa ggagagttat
     5401 ggcttcctga caatctttgt cgcttgtata atgatggttt tacagcgtat attgatttca
     5461 gcagtcacta ttcctacata tgcaacaaac cctggtcgat tctacatcta tacattttta
     5521 caaacttccg ttgttattgg tacaaatttg atcccacttt acatgggtta ccaatatcaa
     5581 aaaatcgccc gtttaaaagt tttacattat ttaagtcgtt ttgcctttat attcctaaca
     5641 gccacactag cttgcaatat ctttttttac gtacaagctg gcagcttgaa tattcgtgat
     5701 tattggctta tcctaacacc tatcagtcag aattacttta cttatgctgt ttcttgtact
     5761 ttgctttttt gtagtattcc ttatacagtt ggatttttaa ataaattatc gaaaacaaca
     5821 attaaatatc tactattatt tttaacaatt ggattagttg gctgctctac tctatttaat
     5881 aaagacccgt ttgcattaca gaatggacag agtattttat ggatttttta tttattttta
     5941 atgggttatg gtttgaaaga atggaactgg aaagaaaagg tccgctataa gcttcctcaa
     6001 gtatttatca gcttgattgt tttattcggt ttgattattt tgatgacaca aatttcactc
     6061 atcatcagag gaaatacaga tacagcgctg aggttttcta aaccatattc tctcttcgca
     6121 atgtattata ctttctcttc gtttttattt ttagaaatgc tatctgataa attaggtata
     6181 aaaggaagag gaaattttct tgctgtattt ttgattgtca ctcaagttat atgtaatttc
     6241 cctttagttt cttatcgcgt atctacattt atgaaacgtg aatttccaaa ctcagggaag
     6301 gcatggctga taaatatcgg gcagtttttt atttactatt tattagcagt gtccctccta
     6361 gttatctttt tattttatat acaaaaaatc cgtttcattc atcgtttcat cgaatacttc
     6421 gcttttgatt cgattgaaca attaacacaa cgttggaaaa aaagaattca ttggcttttt
     6481 gttcataaaa gttatttttt agttggagca tttttttact gctttatctt cgtgcagatt
     6541 ttcgttttag aaccaaaaga aggttggaaa gaaactatcc aggttgcctt aaaaatattt
     6601 actcagcgac aagcacccat gatcctgaca atcatcatta ttatgggatt tttcttctta
     6661 ctattgttga tttccaatcg tttttggtac gccttgactg ctaccttgat cattaactta
     6721 ctgctcacta tttcgacagt aatcaaaatg gaaatgcggg aagagcctgt ctttccttca
     6781 gatttaaaaa tgctgaccgg attgtcagaa ctattatcga tggttagccc agtcttatta
     6841 atcgtgggcg gattgatcct tctcctatta ttgattacta gtattattgt acaacgtaga
     6901 ctacaaaaac agtattcctt gaagattcat tggaaacgtc gatttatcag tatcgctgta
     6961 ttattaggat gtttttcggg tgtctttttc attaatcata aaaattcgcc ttctttcttg
     7021 ctgtttaatt tatttaaagt aaacaagact ttctttaacc agcaaggcgc cgtaaaagat
     7081 aacggaccag tcattcaatt cctaaacaat atcgacatta aaatcatgga agaacctgct
     7141 ggctattcga aagaaaaaat taatggaata atgaaaaagt acaacacaaa ggcaaatgaa
     7201 atcaaccaaa caagaagtga ctggttaaac aatcagacga tcgttctcaa tttaagtgaa
     7261 agcttcagtg atcctgcacg tgtccctaat ctgacagtac ctacagaccc tattccttac
     7321 atcaaaagca ttataacaga aggaacagga ggattgatgt tatccgtcgg ttatggtggc
     7381 ggaacagcta acatggaatg ggaaggactg acaggcttga gtatcagtaa cctgtctgct
     7441 tcgttagtta caccttatac ccagctagtg gagcgtcaga aaataagtcc aaatattacc
     7501 gatctattcg atgaaaaaat agccatccat ccttttacag ctgctcttta tcgcagaaaa
     7561 caagtgtttg ataaatttgg ttttgacagg ttctattatt tagacagtcc ggataaatta
     7621 acttatacgg ataaaataga acaaagccca cgcatatcgg atgattcagc atacaaagaa
     7681 accttgaaag ctttacaaag caatacagaa acgtcgcaat tcatccaatt atccactatg
     7741 caaaaccata tgccttatga tgattactat agtgaattga actacactgc tgaaggaact
     7801 gctgtcattc ctaatagaag aaaagaatta caaacttata tgcaaggcct tcactatacc
     7861 gatacagcag taaaaaaatt catccaagaa atcgacaaaa tcgataaacc gattaccttt
     7921 gttttttatg gtgaccattt acccgcaatc tatagtggga atgattcgaa aaaatatgga
     7981 ttagaacagc accagacaga ttatttcatt tatagcaaca agtatagtcg tgaacaagca
     8041 gctacgttaa ataaagaagt tgttgctcct aataactttg gtgctatggc tcttgagcat
     8101 gcgaatatca aagtaactcc ttactatgcg ttgttgacag aagtatctaa tcatctacct
     8161 gcattaacaa ttgatcctac agaaagcttg tctaatcgtt ttaatggaaa acaggtcttt
     8221 gttacagagg atagtcaaat tatccaagaa aaaaatttaa ctgagaaaca aaaagaaatt
     8281 ctagaagatt atcgtctgat acaatatgat cttgttgcag gtaaccagta cagtgcaaca
     8341 tgggcgacac aaaaaataaa agactaacaa ataaactgag caaggggttg tgataaaaga
     8401 catgtcacaa ccccttgttt atctcattaa tttgcttgtc cataaacgat ttgattaaag
     8461 ccatttttca attcattatt cgctgtctcg ttaaagtaaa gataattatc gggattcccc
     8521 tgattttgtt gtgtcaccaa tacttttggc tgattttgct gaaacccgaa tagatagacg
     8581 tgctttttca gatatggttc tgtctctgca tcggaataga cggctgtgat ttgataatct
     8641 gcttgacctg tcccactttc tgaccattct gcagaaacag gcactccacc aatagccatc
     8701 ttccactccc cgttcaaaat agcttgaggg actttaagac catataagtc cacactcatt
     8761 tgattcgtat agcttttgta ttgttgtccc attgtttggc cccatgtagc cataaatgcc
     8821 tctaattgcg cggatttgtc tgcattccac agtatttgtt cgacttgttc gctgctttct
     8881 gaagatgaag cctctacaga agaacttgaa gtagatgttt gagttgtttc agtcgctttg
     8941 gaagactctg acgaagtaga ggtatctgct gtttccgtac ttgctgcagt tggcttgctc
     9001 tctgtgctag ttgttgtagt agttgcaatt gttgctttcg ctgtttcttt tgttgattct
     9061 tttttcgatt cattcccgtt ggtacaacca gctaacaaaa aagctagacc aattgttact
     9121 aaaatccttt ttttcaatta aaaaactcct ttcgattact cccacttctt tattctatca
     9181 aataagcaat gtgattttat tgattttttg tattttctca tccatctttt aaacctttta
     9241 aaaactaaga agcacccgct gatgttgtct gtaatacatc agcgggtgct tcttttattt
     9301 taatcttcgt gattttccaa tttacctcac gctttatatt tgtatttgaa gaaacataga
     9361 cttttagtca tcatcgtctt cctcatccaa atcttgtcgg acagtttttc cttggagtct
     9421 ttgtacttgg tagatgattt ctttattgaa acttaaaagt tcaacgagta attgttgcaa
     9481 gccatatttt tcttcttttt cagctaactt gattgcccgt gtgataaaca tattttggaa
     9541 atcaaaatct tttgcaaagt tttccaacat tgttgaggct ggtaaatatt tgttttgacc
     9601 atgttcgtaa atttttccat aacgcatgac ttcttctaaa gtagacggaa cgatttcatt
     9661 atcaatcact aactcatgag agattttatt gaaccatctc gcttcttgat cagcatattc
     9721 tgaaaaaagg gtacttaatt cttgcgaaga tgtacccaaa acatacaatt gtgcttggat
     9781 caatttcatt tcttctaatt taaaatttgc taatacatgc cccaccatgg cgccagcagt
     9841 tggtttatga tggtcgatat ctgcttgttt tgtttcttga gcaaattttt cttccactat
     9901 attcactgat ttacgcctct tttacttttg ttgattggac ttcataacct aatttttcca
     9961 caactgcagc taattgttca gggtggactt catctggatc aaagtttgct ttgattttgc
    10021 tcgcattaaa caaaactttc gtattgctta ccccaggctg ttgagaaacc gcatgttcga
    10081 ttttttgcat acagcttgga caagctagtg tatctaattg cataataatt tttttcatcg
    10141 tagttcttcc ttcctttttc ttgatagtct tattctagat tacagcttcc ataaaaaaat
    10201 tgattcttat caagttttac gatttttctt ctttgctttc gtgtccaaaa tacaataaac
    10261 gcatcgcgtt aaagataacg atcaagatac tagcttcgtg aacgaacatc ccactagcca
    10321 tgtaaataaa cccggcaaat aagccgatga gtaaaaataa gacgacacca atggcaatca
    10381 ctacattttc acgggtattt aaaacagttt tcttagccaa gcggtaggca tgaacaaacg
    10441 atccaaaact agattgcatc aagacaacgt cactcgtttc gatagcaaca tccgttcctg
    10501 atcccatagc aatcccgata tcagccgcag ccaaagaggg agaatcattg attccatctc
    10561 caataaaagc aacgcgcaat ccttgttctt ggtatttttt cacaaattga accttttcgt
    10621 ctggtaataa ttcagcatgg acttcatcaa tgcctaacat atcagctaca taatcagcag
    10681 tcatctgatt atcacctgtc agcatcacta actgttttgc tcctgcttgt ttcaacttag
    10741 ctaattgttc agcgacttct ggtcgaatca catctgatac acctatgatt tgagtgattt
    10801 ttttgtcgat tgctacaata atgactgagc tgcctgtttg ttgtaattga tgtaaatctt
    10861 gtacttgctt tggtgtaaga gaaagtttgt gagcggcaat caatttggtg ttgcccgcac
    10921 aaactgcatg accatcaatc tcagctgtga tgccttgtcc cttgattgtc tgattatctt
    10981 tgactgctaa ttgttgaaaa tcaatgtctt tctcctgaac ataatggatc acggcgcgcc
    11041 ccagaggatg atcggataac gtttctaatc gagccactaa agctaagctg tccccttcat
    11101 tctctgtata gttcttgata gtggatacag cagtagttcc tttggtcaat gtaccggttt
    11161 tatcaaagac aaaagtatcg attttggcaa aagtatccat tgcttcaccg cctttgacta
    11221 agatgccccg tttcgctcca ttaccgattc ctgctacatt cgaaacaggc gcaccaatga
    11281 ctaaagcgcc aggacaacca agaaccaaaa cagtgattgc taaacgaaaa tcacgactga
    11341 tcagaccaac aagcaacgca acaagtaaaa cggttggagt ataatactga gaaaatcgat
    11401 caatgaattt ttccgcatgg gatttggtat cttgtgcttc ttctactaat tcgataattt
    11461 tggcaaaggt cgtatcatct ccgaccttag ttgcttcgat cgtcatatag ccatctgcta
    11521 aaatcgttcc agagaagact tcatctccac tgattttgac gacttgtcgt gattcaccag
    11581 tcactgcggc ttcatccgca taaccttttc cgtcaatgac tcgtccgtcc acaggaatag
    11641 ctccaccagt ttttactaag acaatatcgc cttcttccac gtcatcgatt tccacttctt
    11701 cagtcgttcc atcttcttgg acaacactag cagtcgatgg ggccatctcg gtcaaagcct
    11761 tgattgattc ccgcgttttg gtcaatgttt tttgttccag atagctacca aacaaaaaca
    11821 agaatgtaac gatagctgac tccttgtatt ctccgatcat aaaggcacca agcacagcaa
    11881 tagtaacgag taaatcaatt gagataactt tgactttcac tgcctgatac gcatggatca
    11941 taataggtat gaccgcaata atagaagcta tggctagagc aatgttatag aacaagacaa
    12001 atccagtaaa ttttccgata aaaccaaaaa taatcaacac agctgataag gcggtgatat
    12061 gatttcggtg ctcttggatc cacttttgaa atttcatctt tctccctctt ttccttttcg
    12121 ttcttacgag actaagtata gagacttatc catcataaaa aattgaccac tatcaagttt
    12181 agtcaaaaag gcggttttct tttggtagaa tatactacaa aaaatagtta gcaagaagac
    12241 tcactaaaag tgatcttgcc aactattgtc gtttctatca aaattttcat tgaatgattt
    12301 tcctgtacag catacatcgg agatctattt ctttatctaa gatttttatc ttgctaattt
    12361 tttcctttag tctttcaaat ccacgtcgtt catagaattg atacgtacag ccgctatctg
    12421 taaaaaggaa tatttctttt cctttctctc tcttttcaaa ttctgttagc aactgggtgc
    12481 cgatgccttt tattttcaaa tcaggatttg cagctaaaaa aatgatctct ccatctggcg
    12541 aatgcttctt taaatatgct gcaagcattt gttcatttgc tgtctcgtaa gctcctttac
    12601 tttctttagc aaatatattt tgtaaaaaat cgaatatctt aacatatatt tgctgtccaa
    12661 aagaacgatg tttctttttt tctcctttta tctccgccag caaaacacct gccaactcgt
    12721 cctctgcata caaagcaaga atttgagttg ctcgattcaa ctccatatac aaaaaatatc
    12781 ttccgtaaag attcagcaga agttcatttc ccatatatct ctcaaagttc attccttcaa
    12841 cagcaaaata gatagctttt ttgtaatctt tcttttccaa gggtttaaaa gttagtttca
    12901 tcatttcgcc tcctcaactt caatgattgt gttatactta cattataata gacacagtgt
    12961 ctactatcta taatcaatca tctataagat tgtcgattaa gcgacagctt tgctttgata
    13021 tgtcggataa ggagataggt aaatgaaaaa gacagataga cgcgtcaaaa aaacagaaaa
    13081 agctttggct gaagcactat ccacattatt agtaaataaa aagattcaag caatcactat
    13141 acgcgaacta acagaaacgg cagatgtaca tcgttcgact ttttatacac attataagga
    13201 tatctatgat ctatatgatc aattagaaag caactttttt cgggatctaa atgaaattct
    13261 ctcttatgat ccagcccata gctatgagga actatacact cgcttgattg actatgttta
    13321 cgcaaatact tttttatatc gcttatttat cctcaaccat ggtgatattt cgtttcagaa
    13381 gaaagttaaa caagttattg aagaaaacta tctgaacata tggctatatg aagacaagaa
    13441 aacgattatc acagaagaaa tgagattctt cactacgtac cacgtccaag gttgtctatc
    13501 tgttctcaat cgatgggtag aaagcaatta caagtactcc aaaaaagaat tattagaact
    13561 cttacagaaa ctgaatgatc acatagagaa aatcatgcct tgaataaaaa catactaagc
    13621 ttagttaaga agaaccgtaa ccttctcgta taacaactga atcttattta gccaaattgt
    13681 ttactgaaga ttttttaagt aaaatttcta taattcctac ttattgaaaa gtagtttgta
    13741 gtgctgcttc tccctctcta agtgatatat cttgttgtac caatcaaatt cacctcatca
    13801 ttaataaatt acttttaggc taatttatta caacttatag gttgaagctg taaccaattt
    13861 atagtcatat tatattttta tcatccagtt tattgaattc ttctcgttta cctcaattca
    13921 ttttcaagtg gaatcagctc atcatctaac attttgcgcc atgcttgctt catttcttgt
    13981 gggtatttaa atggcttcgc ttctggatgt ttagctttat atccttcgta atccttactg
    14041 ttgataattg ctttcatatt aggaattgga tcttcacctg gcatatattg cagattagaa
    14101 acatgtaatt ttttctcaga aacaaaccac tcattggaaa agacatgaat caaatcatca
    14161 cttacttgtg taaaatagcg acgtttaatg acaaagatat cttcattgct atctagtgta
    14221 ccttcatttc tttgtgctta gattcaaaga taattttctc aataaagatt tttgattcca
    14281 gtaagattcc tttactcctt ttacaacata gcgttgtcct ttgttttgaa acatcttctt
    14341 cccctctttg ccattaaaaa tagcccaatt ctctataaag aagattaagc ttcattatta
    14401 taatataaag tttaaatttg gtttattact tactttttct tattctctag taagctaata
    14461 ttgaaaaaaa tgtaatcaga tcattatttt tatatgttgt aacttcctga taagaaaact
    14521 aattaaaaga aagaggtaac tacataaact aattaaaaaa gctaacttca tagttggagg
    14581 ataaatggaa gaaacttcaa tgattgtttc tccttgtgtt ccttcaatcg acactagtcc
    14641 ttcactatta acaatctctc ttttttgtcc atttactttt gcgactgtcc cccaataatc
    14701 aaatacagga agcgttattt tttgtccttt agattcatgt actgtataga ctattttcga
    14761 atccatcact tgataaggca tagtatgcca ttccttgcca attcttactt catgattctg
    14821 caaatgttct ttgtattgta atgcttccgt gggtgcatag tcataaagtc ctccgggaag
    14881 gagtgtcgtg taacgaataa gattttcttg attcattacc aagtgatctc tctgagtgac
    14941 aactttatgt tcatagtttg cttttgttgc agcagataca gccaacagta ataaaacaat
    15001 tgtcgttata ttaactactt ttctttgctt agctgatgtg ctcttcatga aaacgaacga
    15061 taaaatcaac gagccaaaga gtacttgaaa tcctaagatt cgccaaggaa attgaatcat
    15121 ttgaatgggt gtattttgta ataaagacca tggaaaaaat ttggtggata agagccaaga
    15181 gactaccgca ctaaggtaaa tcactttttc tttgcgatta aatcttttaa aaaagaagag
    15241 tggacagatc aaagaaataa taccaatgat tcctaccgta aaggttgtca ggtcattatt
    15301 catcgcacca atcaaactat ccgagacatt taatgcttgg tgttgtaagg tcgttttatc
    15361 tggctcatta atcgattgaa ccatgatttg tcggaacatc ggataccaaa agtaagctgt
    15421 taaaagaatc gtcattccta ctgcctttga caactccaca atcaaccgga tgctttcttt
    15481 ttttcttgtt tcacttacta caaagccacc taagactagt aagccaacgg ctattacact
    15541 cataaaagtg gaaagaacat gtgtataaat gagtaagctc atactgactg ctaatggaat
    15601 tgcacatggc tttttctcat aaatcagtgc gtaagcataa tacataacta tcggtataac
    15661 cgtaagtgca atcgcttcag caatagcaga acgatgataa atatcaatcg tacggtaccc
    15721 ataaaatgta tataaacagc taaatataaa agccgcatcg attcttttta aaaattttag
    15781 tccataatga aacatcagca tgatagtaca aacattcaat agccaaacat ataagatata
    15841 actaacgatt aaattattac ttatccaata aaaaatgact gccggaaaaa aggtcaagta
    15901 gggataaaaa taattcactc cgctcccata atgattaaac gtcgtaaaat tgattggacc
    15961 agaaaaaatt gaagataacc ctttgactct agcaaaatgg aaggcaatat cccccgatgt
    16021 agaaatagta tgaacatttc taaaataatt gattgaataa agtccagcaa taattgctag
    16081 gagaatggac aatattattt ttttctttac ttgcattact taattatata ctcctttcat
    16141 ggattgcttt atctagtata acttaaattt taaataaatc gttatttatt tttactctca
    16201 cttaaaaaag cactaaaatt ataagagagg gaggtaatgg tctgaacacc aagaattgtt
    16261 ccaacatcga attgcttaac taatgagttc aaagcttaat tatgtggctc ttatatttac
    16321 cgagcgtgga ttgcgatctc tttagcgaaa caaacatctt tcataaaaaa aacagccata
    16381 tccgctttct tggatatgac tatttactac tactttacta tctttcattc ttcttgtctc
    16441 acttatgata aggcgctgcg tatcatgagt gaatgatatt aggaaaactt cataaaataa
    16501 atcttttata catttccact tctagatggt tattc
//
LOCUS       DACCKU010000069        16399 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.072, whole genome shotgun sequence.
ACCESSION   DACCKU010000069 DACCKU010000000
VERSION     DACCKU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.072"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(184..840)
                     /locus_tag="IVN13_002041"
     CDS             complement(184..840)
                     /locus_tag="IVN13_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP7012509.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            1033..1311
                     /locus_tag="IVN13_002042"
     CDS             1033..1311
                     /locus_tag="IVN13_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012510.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1330..2538
                     /locus_tag="IVN13_002043"
     CDS             1330..2538
                     /locus_tag="IVN13_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7012511.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2686..3051)
                     /locus_tag="IVN13_002044"
     CDS             complement(2686..3051)
                     /locus_tag="IVN13_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP7012512.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3198..3971)
                     /locus_tag="IVN13_002045"
     CDS             complement(3198..3971)
                     /locus_tag="IVN13_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7012513.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4145..5005)
                     /locus_tag="IVN13_002046"
     CDS             complement(4145..5005)
                     /locus_tag="IVN13_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP7012514.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(5005..6423)
                     /locus_tag="IVN13_002047"
     CDS             complement(5005..6423)
                     /locus_tag="IVN13_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7012515.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6480..6929)
                     /locus_tag="IVN13_002048"
     CDS             complement(6480..6929)
                     /locus_tag="IVN13_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7012516.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7374..7637)
                     /locus_tag="IVN13_002049"
     CDS             complement(7374..7637)
                     /locus_tag="IVN13_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP7012517.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7736..9031)
                     /locus_tag="IVN13_002050"
     CDS             complement(7736..9031)
                     /locus_tag="IVN13_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP7012518.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(9028..10299)
                     /locus_tag="IVN13_002051"
     CDS             complement(9028..10299)
                     /locus_tag="IVN13_002051"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP7012519.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10464..11333)
                     /locus_tag="IVN13_002052"
     CDS             complement(10464..11333)
                     /locus_tag="IVN13_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP7012520.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11625..13235)
                     /locus_tag="IVN13_002053"
     CDS             complement(11625..13235)
                     /locus_tag="IVN13_002053"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP7012521.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14428..15768)
                     /gene="glnA"
                     /locus_tag="IVN13_002054"
     CDS             complement(14428..15768)
                     /gene="glnA"
                     /locus_tag="IVN13_002054"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP7012522.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15796..16188)
                     /locus_tag="IVN13_002055"
     CDS             complement(15796..16188)
                     /locus_tag="IVN13_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7012523.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4676 a         3475 c         2792 g         5456 t
ORIGIN      
        1 gttttttgaa attgttcctt gtgactcaga gttaaacggc aggctcacaa cctcttggta
       61 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
      121 tatttttccg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctctttgt
      181 tcatcaaaag ataatattgt cttctaacaa gtccatattc aaaataacga tatgatggtc
      241 tttaacggtg ataataccat cttttttcag ttgttttaaa attctgttta cactgctagc
      301 agttgagatg ccacaaaatt tggcgatttc ttcattcgtg atcgaaaaat cgatcagcat
      361 cccttcttct gtccgcacac cgaataaatc atataattcg taaagttgcg tacaaacagc
      421 tcccagcttt ccgtttaata gcatttgctg catttttttc atcgaataaa gcagacgata
      481 gcggtaatac tcttttacat agtgttgtag ctttgggtct tggttgatat tttgccaaaa
      541 gcgcacacga ttgattcgat aaaactctgc atattctgat tcaactcgaa tattatacgg
      601 cgaatcgatg aattgtgaat attcgtcttt taaaagagag acgatttcaa gatctttgat
      661 atagcgaagg ttaaattctc tcccatcatt tgaaataaca cttgctttta ccgttccctc
      721 tttcaagata tacgtatggg aatcttccat cccttcgaat gtcaaatatt tatgatatcc
      781 tttgcgtata atcggaaaat catttttatc caaatattcc tttaagactc tactatccaa
      841 tgatgctccc ccatccaaac tttcatctaa aaagatcata cagtacttta aaacttttta
      901 aagtccaaag gtattttact aatgatatta acatttgtta agtacctttt tccatcactt
      961 atgcgatttt tttccttttt tttattcgtt atactgcaga agtaaaattt tttaagaata
     1021 gagggaaaaa acatgaatta ctggatgaac acaatcatta atcgattaga aacagcatat
     1081 caaacaagat ttgatatgaa agcttcttta gtctttttaa atgatgcgta tcaaaactcg
     1141 atcgaattga tcaaagcagt ggatgagaat ccaacaaacg aatgcgaaga atttttaaat
     1201 ctgtttatgt ctacaagaga tttatttatt cgccaacttg ttgatcgcta tccatccaat
     1261 tatcacgatg tagaagttca aatacaaaaa ttaaaagcat attcagctta aggggaatgt
     1321 ataacataca tggaaaagaa attatttaat aaatatttta ttggaatcac attgatcaat
     1381 tttattgtct acctgattta ttatctgttg atggtcatca tggcagtcat tgcgcaagac
     1441 accttacacg ccagcttgag ccaagcaggt tttgcttctg ggatctatat catcggaaca
     1501 ttgctggcac gcttattcat ggggaaacag ttagaactat ttggtcggaa gtttacctta
     1561 agaggcggcg cgatcttcta tctcctttca acattagcat atctttatac gccaactatc
     1621 ggggcattgt atttagttcg attcttgaat ggattcggct acggtacagt ttctacagct
     1681 acaaatgcta tcgttactgc atatatccct gcatctaaaa aaggggaagg catcaattat
     1741 tatggattaa gtactagttt agctgccgct gttgggccat ttttaggaat gattttgctg
     1801 aatatgactg attttcgttt cattatctgg ttttcaattg tactggtctt ctttgttacg
     1861 attgcctgcc ttgtctttcc tgtaaaaaat attccattat ctgaagaaca aaaaatatct
     1921 cttcgctcat ggaatatcaa tagttttatt gaaaagaaag cacttttcat tacaggaatt
     1981 gcctttttga tgggtctttc ttattcaagt gttttatcat ttttatcttc ttatacaaaa
     2041 gtaatccact tagttgctgc tgggtcattt ttcttcgtcg tatatgcttt ggtcatcaca
     2101 tttacacgtc cattgactgg aaggattttc gatgtcaaag gagaacaata cgtgatgtat
     2161 cctagctatt tattcttaac agcaggatta tttttactga gtgtaacgac gaatagtgtg
     2221 acattgctta tctctggagc attagtcggc ctaggttatg gtacgtttat gtcaaatgga
     2281 caggctgttt gtttaaaaat cgtccgcgaa catcgaatca gtattgcttt atctacctac
     2341 tttattggtt tagacctagg cttaggcgtt ggtccttatt tgatggggat gctaaaatct
     2401 cttacttcat ttagaggatt gtatgtgatt gccggagtca ttccattgat atgtacagca
     2461 ctctatcttg ttaatggccg aaaacatagt catgaagcag agggcgaatt gattgaatca
     2521 ttgaaaaatg aaatttgatc ttgatgaata gataaaaaaa accgttcaga tctgaaaata
     2581 atctgagcgg ttttttgagt aaaaatagtt tatgattatt cttaattaaa catctattta
     2641 tttttaaagc tttcatttat cgataagaat agtttaactg ttgatttatt ccattttccg
     2701 ttcatctggt acgatgcctt gacttagcaa ccacttgtgg aagaaccatt ctgccacacc
     2761 gatcaataga gcaggaatca atactttcat ccaaatatcg actgtatcat tcataaaata
     2821 tagatagagc cacaagataa ctgcagctga accagcatct gcgatcgtcg ctgcgatgtt
     2881 ccctacttta cgtaagatca acagatcccc tagcacatag atcatgacta caataattac
     2941 cccaacaatc gttgaatcca taaagctaac accgtataaa cctgtcagca cgacccacag
     3001 aatcgcccag atcatgattg ccttcactac caacgccttc acatgtttca ttttctgcac
     3061 tcctttctcc tggataaaaa agtaagatta tctaacaaga taaccttttc attattatac
     3121 catcggctga gagaagcgag caaagataag gtcctatcta aaaatgagga agccacttag
     3181 aacattcacg taataattta tccgtacaga cattcgatat ggtgttcctt tactttcgca
     3241 tactgttctt ttgtcagttc tttacttgtc accagataat cgatttcatg aaaagcaatg
     3301 gcttgatgca tgaatttatg tgcaattttt tgctgtgtaa ctaaaacggc aagccggtct
     3361 gattgttttc taaaagcttt tttgaccatt gcctctcctt catgagtttc tgttactcct
     3421 tggctgaaat ctagcccgcg acaactcatt atcgctaaat ccgcataata tgacaatgca
     3481 tcgttatagg cttttgaacc gttgattgtt gctcgtttcg gaacaattgc ccctcccaat
     3541 acagatactt ttgccgttgt atattcactc aacatagcag ctgtgacgat gccattcgta
     3601 atgatctgca aaccatcaaa tcttgctagg tttctgacta agtgtaaaga ggttgtactg
     3661 gaatccaaga ataagaccat atcatctcga atcaaagaag aagctgtatc tgcaatctta
     3721 cgtttttctg tttcttcttc taatttggtg atcttcatca ggtaaggcac gtcaattttt
     3781 cgattgtcaa taagcatggc gccaccgcga actctacgga tcaatccact tttttccaga
     3841 aaaacaaggt cacgtttaat cgtggcttcg ctataattta ctaactcgat caaatcggtg
     3901 actcttgcaa attggttgtt ttttagataa ttcacaatct cctgctgtct attctccact
     3961 tcattcaaca taaaatcacc ccatttactt tatatagtaa caggaaaaaa gaggttgtga
     4021 cagaagtgat tggcttcgag aaataagaag aaatttttcg aaaaaactac ttctgaacat
     4081 cgtgtgtggt taagagggta tgacaagact attgtcatac cctcttgacc acacattttt
     4141 atttttagta taaactagcc ttgcctactg tgttcaaaat ttccatttta tgtttgacca
     4201 cttcttgtac ctttttatta gctgctggat aaattgcatt cggctcgtac atcgatggat
     4261 tttcactcaa tatttctctc aaagcattga aaaagacgct tttaagatct gagctcaaat
     4321 tcactttggc aactccgtat tttactgctt cgctgacttc tgcgtccgga ttacctgaac
     4381 cgccatgtaa aacaaatgga agatcgattt gttggttcaa ttctttcagt aagtcgatat
     4441 ttaattttgg agttctgttt ttcggataca agccatgcgc tgttccaatt gctacagcaa
     4501 gtgtatcgat tcctgtttct ttcacaaaaa tcttcgcttg atcaggatct gtataaatga
     4561 ttgtttcagc acctccttca gcagaaccat tattcccaat cgttccaagc tcagcctcca
     4621 ctgagacatt taccgcatga gctaacgcaa ccactttttt cgtaatctgt acattgtcgt
     4681 cgaatgattt caaagacgca tcgatcatca cggaagaata gccgtttcga atcgcccgta
     4741 agacatcttc tatcgtaccg ccatgatcca agtggataac gatcggcagt ttacttgtat
     4801 aagcataggc tcttactgcc gcaacaaact catcacttaa ataggcgatt tcatcaggat
     4861 gaatttctaa gattactggt gcttctaatc tctctgcttc ttcgatgatt gctttcatca
     4921 tatcatagct gcaaatattg aaggctggta ctgcaaattg attctcttta gccactttca
     4981 ataactcttt cattgtatat aacattacgc ttccacctca tttgttagat tttcattttt
     5041 cgtccacttg tcttgttttt tctctttcac tgtttttttc aatagtccaa gcataaaggc
     5101 tgtagtgact gtacctgatg caattgccaa gacatacata ggtaaattgt tcataacatt
     5161 tggaattagc atcacccaaa tccctccatg aggtgccatt gaagtaactc ctactgccat
     5221 ggaaatccct gcagcgactg ctgaacctgt catcaaacta ggtatcacgc ggatcgggtc
     5281 agataccgca aacggtatag caccttctgt aatatacgaa gcgcctaaaa tccagcatga
     5341 ttgacctgaa gtccgttctt ctttggtaaa tttatgtttg aataataaag tggctaaagc
     5401 aatacctagg ggaggtgtca ttcctgcagc catacaagca gcaatcggtg catttactcc
     5461 agctgacatc aaaccgatcg agaaagtcga agtcgattta ttgatcggcc cacccatatc
     5521 agctgccatc atgatcccga taagtaatcc gaacaatagc ccggatgtat ttcccaagct
     5581 attcagccaa tttgtcaaag catccaacac ccatgtgatc ggttgttcaa tcacaaatac
     5641 catcaaaagc ccaataatta agatcgataa aaaggggacg acaatcagct gataaatagc
     5701 agaaaattct ttttttacgc taattttatt tgccagaaaa tcacagatat accctgctag
     5761 cacgccacca atcattccgc caagaaaacc tgaaccacct gtagaggcca gtaatccggc
     5821 aacaattcct ggggcaaaac ctgcttttcc agcaatcgat gtagcaatcc ccgcgcctaa
     5881 cgcaggaacc atcatggcaa acgctgtatc tcctccgatc cttgaaaatg cagctgctaa
     5941 cacattatag tctgctgagt tgggatcagc cgctgtaatc ccaaaggcaa aactcagtgc
     6001 gatgatgatt ccgcctgcga tcacaaatgg aagcatgtaa ttcacaccat tcatgaaatg
     6061 attatagatt gtcggctttt tctcatttgc agctggttct ttttcattag acacaacgaa
     6121 tacgccaata tctctcaatg cttcgtctat tactacttgc ggttctttga ttgctcggct
     6181 tgtagagaca cgctttactt ttttcccggc aaatcggtcc aattcgattt ctttatctac
     6241 tgcaagaatg attgcatctg cctctttgat ttcttgttca gataactgat tttctgtttt
     6301 tacaccttgt gtttcgaatt ttgcttgatg gtttagattt cttgctgtta cttctaattt
     6361 ttctgccgac atataagtat gagcaattcc agtcgggcaa gcagttacac caacgatttt
     6421 catgattatt ttcctccttt ttcttcacta tcgatactcg gacaaagact ctactatttt
     6481 cattcttgac taatctgtct gatgatatct gctggcgtag tggcttcttt tattcccttt
     6541 ctaaaatctg cattcatcaa tcgtctggag agtgtgctga gaatctctag atgcatatct
     6601 gctgattttt caggaactac gatggcaaaa atatattcaa ttggcatttc atctatcccg
     6661 ctatagtccg ttaatttggc tgtatgaaaa aaacacagtg ctggttttgt cacatatttg
     6721 ctttttgcat ggggaatccc aaatccatct tctattcctg tggagatctc ctcctctcgt
     6781 ttgaatagtg ccttaacgac tttttgaaca tttcttacct tatttgtttt tttcaacaca
     6841 tcccctattc gctcaaataa tgatcctttt gaatctatcg atggatcgaa aacaatcagt
     6901 tcttctgcga tcaattgatc aaacctcata aaaaacctct cccccacaaa aaatgttaac
     6961 gttttcttgg aactgaggtc agtatagcat cgctagctgt accgttcaat attatgaatg
     7021 aacagaggaa aaagacccat catccgcgtg tataagtcat taaaatgaac gatctagctc
     7081 acaaacaatc gcaagtctat aacaaaacaa aaagccttcc ttgaaaggaa agcttagctt
     7141 ctaattttag gtaaatggtt ttttacttcg aaataacccc tcttcatttc gttaatataa
     7201 ggtgttttta tcaagtggat aattaaaact atatactagt ttttcattgt tttgtgcatt
     7261 atgatcctac gactttttca gcatcaaacg aagttttaaa ttggcaaaaa acataaaaaa
     7321 acagactatc cctatcagat agtctgctaa acgttgagtt atcagcgttt ttattattcc
     7381 gcagcgtttg cgtctttgag accgtatttt ttgttgaaac ggtccacacg tccgtctgct
     7441 tgtgtgaatt tttgacgtcc agtgtagaat ggatgtgagt cagaagttac ttcgacacgg
     7501 atgactgggt atgtattacc gtcttcccat tcaactgttt cttgtgaagt ttttgttgaa
     7561 cctgacaaga atttgaaacc agttgttgaa tccatgaata ctactgggtg gtaatctgga
     7621 tggatgtttt ctttcatgat tttttcgctc ctttgccctg ctccatttgc tgaaccagag
     7681 ttgatttgtc gatttacaac attaacatct taacatgttt cagtcaaggt tgcaattatc
     7741 ttttcagatt tctggtcgga ttttttttac caaaggagac atcttgaaat tctttgaaaa
     7801 attgctgatt gttttttgtt ttacgcaaaa agcggatcag ttgttccgta tattctaatg
     7861 aatctccagt catatttttt cgaagtttcc aaatttcttc taattcttca cttggcatca
     7921 ataattcttc ttttcgggta cctgattttt tgatatcgat ggctgggaag atccgacgtt
     7981 cagataattc acgtgaaagt tgtaattcta gatttccagt tcctttgaat tcttcataga
     8041 tcacgtcatc catacggctt cctgtatcga ctaaagcggt cgctaaaatc gtcaaactgc
     8101 caccttcttc gatgtttctt gctgccccga aaaatttctt aggtttatac aatgcggctg
     8161 ggtcaatccc gccactcaat gtacgtccgc ttggtggaac gactaagtta taggcccgag
     8221 caagtctagt aatactatcc atcaagataa cgacatctcg tttatcttcg actaaacgca
     8281 ttgcgcgttc caatactaat tcagaaactc gagtatggtt ttgcggctgc agatcaaatg
     8341 tagaagatac cacgtcgcct ttcacgcttc gttctagatc cgttacttct tccggtcgtt
     8401 catcaatcag tagaacgatt aattctactt ccggatagtt ttcggtgata ccatttgcga
     8461 tttcttttaa tacagaagtt ttccctgctt ttggcggtgc gacgatcaat ccacgctgac
     8521 caaaaccgat tggagaaaag atatcgatca ttcgggtaga tagtctttgc ggtgttgttt
     8581 ccagttgaag ctgtctttgc ggataaagag gtgttaaggc tggaaaatgc ggacgttctt
     8641 ttgcttcttc cggatctttt ccattaacgc tttccacgtg catcaaacca taataacgtt
     8701 ccgattcttt aggtggacgg gcttttcctg ccactttgtc cccatttctc aaactgaatc
     8761 gtctgatttg tgaggcagaa atatagatat cttctgcact tggtccataa ttgattggcc
     8821 gtaaaaaacc gtaaccatct tgaccaacga tatccaatat tccttccata taataaaatc
     8881 cttgtttctc tgcttgtgca cgaatcacag ctagagataa ttccttttta ttcatcttac
     8941 tgtaataagg aattttaaat tctttggcat aactataaac ttcttttaac gttttgttct
     9001 ctagttctgc cattgtcaaa taatcgctca ttcagctccc acttcctcta tcatttcaat
     9061 tgatgcccct aaggctgtta gtttctcgat aatatgatca taaccgcgta agatataatc
     9121 tacattgtaa atcgtcgttg tcccttctgc caatagtcca gcaatcacta aacaagcacc
     9181 tgcccgaaga tcagaagcca cgacttctgt gccgtgaagt ttatttggac cattcacgat
     9241 gatcatattt ccttccacgg taatatcagc gcccattcgt gctagttccg gcacatggtt
     9301 cacacgtttc gcatagatcg tatcgatgat ttctgaagtg cctgtagtca tcaatagtaa
     9361 cgcagtaagt ggctgctgca gatctgtggc aaatcctgga taaggatagg tcatgatgtt
     9421 tgcgggtttt aattctttcg aggggtaaac ttcgatttca tcttcgctga ttttcatatg
     9481 gacaccaatt tcctgcaatt ttgctataaa gctttccaaa tgttcaaaga tcacattttt
     9541 cactttgata ccatttccta ctgcagctgc taatgctaga taggttcctg cttcgatgcg
     9601 gtcaggaatc atgaaatgcc gacagccatg taatgtttcg actccttcga tacggatcac
     9661 atctgtaccc gctcctcgaa cttttgcccc catattattc agtaaagttg ccacatctat
     9721 gatttccggt tctcgtgcag cgttttcaat tactgtctgt ccttttgctt ttacagcagc
     9781 taacatcaca ttgatcgttg ctccgactga aaccacgtcc atgaaaatcc gattgccacg
     9841 caatccttta ttttctgtcc gtaaatacat ggcaccatgt tcatttgtca cttcagcacc
     9901 tagagcttca aatcctttga cgtgtaggtc aatcggtcta gggcctaaat aacagccacc
     9961 tggcaggcct actaccgctt cgccaaattt accaagcagt gttcccataa aataataaga
    10021 agcacgcaaa ctattgattt ttccgtgcgg catcggaatc gatacgactt ctcgaggatc
    10081 gatttctagt atgttgttgg aaaaatggac agttgcgccc atgatctcta aaatttcaat
    10141 taatgaatga acatcttgta tgtccggcac accttccaag atcactgggg aatcagctaa
    10201 gattgcagct ggaatcaatg cgactgcact attttttgca ccgctgatcg ttacttcacc
    10261 agctaaaggt cggtttcctt tgattacgat ttttttcatc tgtttccacc ttgtttatta
    10321 atcttttttc atggatatgc ttgtatttta tcatagataa aaaaaagaaa cgagcatggt
    10381 ctgccgtttt aagtaaaaaa gaatggagag cgacttcaaa agtcgttctc cattcctata
    10441 tatcgtatct gttatgtgga tgcttacgct ttgttagctg aaccgaacca gtcgatatga
    10501 gatttagcat cttcaacgat tgctgcttta cctggagcta ataatttacg agggtcgaat
    10561 cctttacctt ctttatcttt accagcttcg atatatttac gagtagctgc agcaaatgat
    10621 aattggaatt cagtgttaac atttactttt gagataccca ttgagatagc tttttcgatt
    10681 tgatcttgag gaatacctga tccgccatgt aatactaaag gcatgtctgg acctacagca
    10741 tcagcaattg cttgtaagtg gtcaaaagct aatcctttcc agttttctgg atattgacca
    10801 tggatgttac cgataccgca agctaagtag tcgatacctg tagcaaccat ttgtttgcat
    10861 tcttcaatat cagctaattc gccgttaccg atgattccgt cttcttctcc gccgattgaa
    10921 ccaacttcac attcaactga gatacctttt gcatgtgctt tttcaacaac gtctttagct
    10981 aatttcaagt tttcttcaaa tggtagatgt gaaccatcga acattactga agtatagcct
    11041 aattcgatac attccaaagc agcttcgtaa tcaccatggt ctaagtggat agcaactgga
    11101 acagtgatgt tcattgcttc aactaaatca gtgattaaat ctttacagat tttgtaacca
    11161 cccatgtatt tagctgcacc cattgatgtt tggataagta ctggtgcttg ttttgcttca
    11221 gccgcttcaa ggattgcttg agtccattcc aagttgtttg tgttgaatcc gccgacagcg
    11281 taaccgcctt tacgagcagc ttttaaaaat tcagctcctg atactactgg cataataaaa
    11341 ttcctcctaa gttttgtaaa actaaatttg ttaagacaat tcttaaccaa cgaccctatt
    11401 ttaccaaaca tttcggtagt tttctactaa aatcaatgaa aatgaaaatt ttcaagaagt
    11461 tttttgcaaa aaataaaata tccaatagcg ctttaatcga aaaaagtctt ttgctttcta
    11521 tcttttttcc tacaaataat aacaaatcag aaaaagagaa aagtgacaag aaaattgctt
    11581 atccccttct cttgctactt ttctctttct atcaagttca aaaattattt tgtttcgtga
    11641 tctgctaatg atgctccgac aaatgctttg attaattttt gcgggcgatt tggacgagaa
    11701 atcaattctg ggtggaattg gcatcccaca aagaattttt tctctggcag ctctacgatt
    11761 tctactaaac ggttatctgg tgagacacct gaaaagacta gcccttgttc ttcaaacaat
    11821 tgacgatatt tgttattgaa ctcatagcgg tgacggtgac gttcttgcac aacatcggct
    11881 ccatcatatg cttcagccgt cttcgttcct tttttgattt tacatgggta taatccaagg
    11941 cgtaaagttc cacccaaatt ctcgatgttt tcctgatcag ccattaaatc aataatattg
    12001 tttgctgtat ctggattcgt ttcagctgat gacgcatctt ctaatccaac gacgttacgt
    12061 gcaaattcga cacaagccat ctgcatacca agacaaatac ctaaaaacgg aacgtcattt
    12121 tcgcgagcat aacgaatcgc ttcgattttc ccttctactc cgcgatcacc aaatccacca
    12181 ggaaccaaga ttccatctgc gtctttcaaa cgttcttcta cattttctct cgttaattct
    12241 tgtgctttga tccaatcgat ctcgatgtct gaattatagg cgaaaccaga atgtttcaat
    12301 gcttcaacta ctgagatata tgcatctggt aactcgacat atttaccgac aagcgcaatc
    12361 cgtacttttt tcttcaagtt caagacacgt tcttctaatt ctttccattc tgtcatgtct
    12421 gcttctggtg tatctagttt taaatggtcg caaacaattt gatccattcc ttgtgcttgt
    12481 aaaactaatg ggatggagta aagtgtatct acatctggcg actcgataac tgcttctggt
    12541 tcgacatcac aaaattgtgc caatttgttt ttcacgcttt gagaaacagg ttgctctgta
    12601 cgtaccacca aaatattcgg ttggatacct aaaccacgta gctcttttac actgtgctgc
    12661 gttggtttag ttttcatttc tccagcagct ttcaagtaag gaatcaatgt tgtatggata
    12721 tacataacat tatctgtacc gacatctgct ttcatttgac gaagtgcttc aaggaatggc
    12781 aaagattcta tatcccctac tgttccccct acttctgtaa tgatcacatc tgaatcagtc
    12841 attttagctg ctcgcatgat tttttctttg atctcatttg taatatgagg aataacttga
    12901 acagttgctc ccaagtattc gcccttacgt tctttacgca aaacttcaga atagattttt
    12961 cctgtcgtca cgttggagta tttgttcaaa ttgatgtcaa taaaacgttc gtagtggccc
    13021 agatccaaat ctgtttcagc accatcatct gttacgaata cttctccgtg ctgatatggg
    13081 ctcatcgtcc ccggatccac gttgatgtat ggatcaaatt tttggatagt tacttttaag
    13141 ccgcggtttt tcaataagcg tcctaaagaa gctgcaacaa tcccttttcc gattgatgaa
    13201 acgacaccgc cggtcacaaa aatatatttt gtcataagtt caaaaactcc tttttaattt
    13261 atgtgcagga aaagtctcag acaataaaca aagctcccta ttcttcaata agaataggga
    13321 gccgaaattt ctgtaaactt ctgaccctct aaaagggtgc ccaaggtgta taatacaagc
    13381 tgactaaggg aaagtcaagt actcaaatga atactatccg cttacaattt tttctttttc
    13441 gatatccttt gagaaattat ttcttaagtc ctttatgagt gcaaaaaaaa tttaacccac
    13501 ataataagct tgatataaac atagctaacg ttaaacctaa gcaaatcaga aaatcgttac
    13561 tgaaaaattt taagtgaaat aatagataaa aaaataaaac aaacattatg atagataata
    13621 tcccacctaa caaagaacta ctttttccta cttgcagcaa cagtttatat agcttatcgt
    13681 agaaacaatc tttttctttc acccttattt acctccttta cgtaaaaaac gtagtccaca
    13741 gttctcggat ttttggcata tcttttctat taaataatat caaaaacaaa acaattccac
    13801 taattttttc aaagatgacc tttttttcaa caaaagtgcc ttgtagggat tcattgaagg
    13861 taacgaatca cataagtaaa aaataaggag atgcgacaaa agtcgtgcct ccttatttct
    13921 aaataaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatcc atcaaacatg
    13981 agaagctgtt tttgaaatta tttcttgtga ttcggagtta aatggtaggc tcacaacctc
    14041 tggataaacg gtattcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
    14101 agaaagccat tttcacaaaa tttttcttaa tactcgaaac taaacgtctt tttcaaaacc
    14161 tcttggtaaa cggtgttcaa aagcttgcat ctgcggtaat aagttcagcc aattcaaaaa
    14221 caagaaaagc tgtttttgct tggctattct tattacttgt tgcagggcag ctttttcaca
    14281 acctctccac taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa
    14341 aatatgagga actatttttc cgttagtttc tctaatactc aggtcaaaac gctgatttca
    14401 cagcctctaa ttcacggtat tcctgtttta ataaagttct agatattgtt ctctttccca
    14461 ctcggaaaca gtttgtctga atgccgccca ttcgagacgt tttgcttcaa caaaatttga
    14521 gaagatatgt tgtcctaatg cgtcaatcat tacattatcc ttacgcaatt ctttgatagc
    14581 attatggata gttgaaggta agtcctggat atgtgcagct tctctttctt cctcatccat
    14641 tacataaatg ttacggtcaa cagctgctgg cggtgtgatc tgattacgga taccgtctag
    14701 tcctgcttgc aacaatactg ccattgccaa gtatgggttt gcagatggat caactgaccg
    14761 caattccaaa cgtgtggata aaccgcgaga ttctggtaca cgtactaatg gcgaacgatt
    14821 acggccactc catgcgacat aaacaggcgc ttcataacct ggaacgagac gtttataaga
    14881 attgactgtt ggattgcaaa cggctgtgta ggcacgagca tgtttcagca aaccacctaa
    14941 gaaatgataa gctgtttcac ttagttccaa ttcaccgtct ttatcataga aaacatttcc
    15001 ttcatcattg aacaaagaca tattgcaatg cataccagaa ccattgatcc caaataacgg
    15061 tttcggcatg aaagtcgcgt gaagtccatg cttgcgtgca atcgttttga caacgagctt
    15121 gaaggtttgg atattatcac aagcatctac cacatctgca tatttaaagt caatttcatg
    15181 ctgtcctggc gctacttcat gatgtgaagc ttccacttca aagcctagac tttctaattc
    15241 caacacgatg tcacggcggc aattttcacc taaatctgtt ggtgcaaaat caaaataacc
    15301 acctttatca ttcaaggtcg ttgtgatccc accgtcttcg tctaatttaa ataagaagaa
    15361 ttctggttca ggtcctaaat tgaaagaggt aaaaccaagg tcgcgcatat ctttcaatgc
    15421 tcgtttcaaa ttcccgcgag gatcgccggc aaatggtgtc ccatccggat tatagatatc
    15481 acaaattaaa cgagcaactt ttccatgagc actttcccac gggaagatca tccaagtgga
    15541 gagatctgga tacaaataca tatcgctttc ttcaattcga acaaatcctt caatcgaaga
    15601 accgtcaaac atcattttat tttctaatac tttgtcgatc tggctcactg gaacttctac
    15661 atttttgatc gtacctaaga tgtctgtgaa catcaatcgt aaaaatcgta cgttttcttc
    15721 ctcaatgatt cgtttgattt cttcacctgt tatctgtttc tttaccatgg tatccctctt
    15781 ccttttttaa tttattcagt caaagaattg tctttgcaat ccttgcgact gataatgacc
    15841 tgttttgctt aacccacctt gcgccaaaat ctcatcacga aaaatttgtc gaacgtcatg
    15901 atctgtcaac ggcttcttgt tttttgcttc ttgttttttc gcctgtgcca tttcgtatac
    15961 acgtttgatc ccagccatat tcaatccctc tgaaagatag tctttaatct caagcaacat
    16021 atcaatatca ttcagcgaat acatacgtcg gtttccttcg cttcgttccg gatgaactaa
    16081 atcttgttct tcataatagc gaatttgccg agcagacaaa tcagtcagct tcataactgt
    16141 accaattgga aatacggaca tagatcggcg caattccttt tctcccatca gacgtcccct
    16201 ttcgttttat aagttggcta aagtgtacca attaaaaaaa atattgtcaa ctgcatatgt
    16261 taggttttct aacatgagat ctctattttt tataaaataa caaaaaaata agaacacaaa
    16321 aaaggtatct agttgatttt cgtaccagat accttttatt ttttattata ctgtactatc
    16381 ctttattagt gtttttatt
//
LOCUS       DACCKU010000070        16102 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.073, whole genome shotgun sequence.
ACCESSION   DACCKU010000070 DACCKU010000000
VERSION     DACCKU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.073"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            110..592
                     /locus_tag="IVN13_002056"
     CDS             110..592
                     /locus_tag="IVN13_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012524.1"
                     /translation="MRRKTVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            789..1679
                     /gene="pstS"
                     /locus_tag="IVN13_002057"
     CDS             789..1679
                     /gene="pstS"
                     /locus_tag="IVN13_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAP7012525.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSTTQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            2004..4475
                     /locus_tag="IVN13_002058"
     CDS             2004..4475
                     /locus_tag="IVN13_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7012526.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLETCIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(4578..6314)
                     /locus_tag="IVN13_002059"
     CDS             complement(4578..6314)
                     /locus_tag="IVN13_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP7012527.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKVQLLIWQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(6311..7015)
                     /locus_tag="IVN13_002060"
     CDS             complement(6311..7015)
                     /locus_tag="IVN13_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7012528.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            7146..7649
                     /locus_tag="IVN13_002061"
     CDS             7146..7649
                     /locus_tag="IVN13_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP7012529.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            7609..7947
                     /locus_tag="IVN13_002062"
     CDS             7609..7947
                     /locus_tag="IVN13_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP7012530.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            7968..9386
                     /gene="ffh"
                     /locus_tag="IVN13_002063"
     CDS             7968..9386
                     /gene="ffh"
                     /locus_tag="IVN13_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP7012531.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKNFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            9498..10076
                     /locus_tag="IVN13_002064"
     CDS             9498..10076
                     /locus_tag="IVN13_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP7012532.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            10080..10601
                     /locus_tag="IVN13_002065"
     CDS             10080..10601
                     /locus_tag="IVN13_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7012533.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTSIMGRKTYQEAKKHLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(10680..11234)
                     /locus_tag="IVN13_002066"
     CDS             complement(10680..11234)
                     /locus_tag="IVN13_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7012534.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            11487..11762
                     /gene="rpsP"
                     /locus_tag="IVN13_002067"
     CDS             11487..11762
                     /gene="rpsP"
                     /locus_tag="IVN13_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP7012535.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            11774..12025
                     /locus_tag="IVN13_002068"
     CDS             11774..12025
                     /locus_tag="IVN13_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP7012536.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            12150..12941
                     /locus_tag="IVN13_002069"
     CDS             12150..12941
                     /locus_tag="IVN13_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012537.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSLEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTKGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            13111..13632
                     /gene="rimM"
                     /locus_tag="IVN13_002070"
     CDS             13111..13632
                     /gene="rimM"
                     /locus_tag="IVN13_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP7012538.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            13622..14383
                     /gene="trmD"
                     /locus_tag="IVN13_002071"
     CDS             13622..14383
                     /gene="trmD"
                     /locus_tag="IVN13_002071"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP7012539.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            14519..14866
                     /gene="rplS"
                     /locus_tag="IVN13_002072"
     CDS             14519..14866
                     /gene="rplS"
                     /locus_tag="IVN13_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP7012540.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            15125..15418
                     /locus_tag="IVN13_002073"
     CDS             15125..15418
                     /locus_tag="IVN13_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP7012541.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5383 a         2885 c         3276 g         4558 t
ORIGIN      
        1 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
       61 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
      121 aacagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
      181 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
      241 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
      301 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
      361 cttattagta tttacatcta tgttttcttt gctgcctcgc ttgattatcg gccctaatgg
      421 tttgagcgag agtggattga aggattcagc ttattcctcc atgtatttct tgacgatcaa
      481 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
      541 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
      601 catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
      661 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
      721 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
      781 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
      841 gcggctcagg atcaggaaat acagatggct ctaccacaca aaataattca agtgactcaa
      901 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
      961 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1021 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     1081 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     1141 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     1201 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     1261 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     1321 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     1381 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     1441 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     1501 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     1561 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     1621 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     1681 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     1741 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     1801 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     1861 aactgtcgta gctttttttt gctatatgac aggaatgaaa ggtgtttgca agcttaatgt
     1921 gaggaacact ttccatatct gttctatact ggataggaga agtttggaaa tatccagaaa
     1981 acgaaatcaa tcgaggtagc cacatgaaga ataaatggat aaatcgaagt aaaaaaaaga
     2041 aagtaacaca aagatcatca gataaagtca tccgtctttt tccatcgtat aaagaaggat
     2101 taacggcaga tcaagtcaag gaacgaatag aaaaaggtgc agcaaataat tcagtcgatc
     2161 ccacattcaa aacaaatcag caaatcgtat tggaaaacat atttacgtat ttcaatttga
     2221 tctttcttat cttagccatt ttgctttgtt tagtagaatc ctataagaac ttgacttttt
     2281 taccagttat catagccaat acaggaatcg ccatttttca ggaaattcga tccaaaaaga
     2341 tcttggacga actaaatgtt ttaaatacag caaaagtcaa tgtcatacga gacgggatag
     2401 aacaaacaat agatatagaa gaccttgtaa tagatgacat tgttatttta gagactggac
     2461 atcaaattcc agcagatgca gaggtcgtgg aagggaaact gcaagttaat gaggctttat
     2521 tgacaggcga agcagatgaa ataaccaaag aaataggcga tccattaatg tccggcagtt
     2581 tcattgtttc cggaaaagct tacgcccgat tagacaaagt tggagcagat tcgtatattt
     2641 cccagctgac agtcaaagcc aagacaatgg gagaaggaga acagtcggag atgattgctt
     2701 ctcttaatca attgatcaaa tggattggga tcatcattat tcctatcggt atcctgcttt
     2761 tttctcaaag ttactttttt aatggaaaca cgatcaaaga aagtgtcgtt gcaatggaag
     2821 cagcattgat cggtatgata cctgaaggac tgtacttgct gacgacgatt gcattagcat
     2881 taagtgcgac taaactagca aagcaaagag ttcttttaca caatatgaaa agcattgaga
     2941 cattggcaag ggtaaatgtc ctttgtgtag ataaaacagg aacgatcaca gaaaataaaa
     3001 tgtctgttca aaaagtgatc gtctctaaaa aacaagaaat aatcagtcag tcagatcaat
     3061 tggaaacatg cattgctgac tatgcaaaag cgatggcagg agacaatgca acaatggaag
     3121 ctgttaaagc atattttaca aaaaccactg atcaatccta tcaccatgtt attccttttt
     3181 cttctgtcca aaaattcagc agtgtagcgt tggcagacaa ggtgtatgtt ttaggagcgc
     3241 ccgaaatggt tttgcgcgat caattgccgg aatatgcgga agagtttgtt tctttcgctg
     3301 aaaaaggcta tcgcgtgttg gtctttggtg tgtacgatgg gattcttgaa gaaccagtat
     3361 tgacagaagc agtcacacca ttaggctata ttctgattgc taatcctatc agaaaagaag
     3421 caagagccac atttgattac ttcaaaaaac agcaagtagc catcaaagtg atttctggag
     3481 ataatccgct caccgtatcc aatgtagctg tccaagcagg gatttcacat gctgatcagt
     3541 acgttgatgc atctaaatta tcagaagaag aatatgaagc agctgtcgaa aaatatacag
     3601 tttttggacg cgtcaagcca gaacaaaaga aaatcttcgt tcaattgcta aagcgtaaca
     3661 atacagtagc tatgacaggt gatggcgtga atgatattct agccatgaaa gaagcagact
     3721 gcagtatagc gatggcttcc ggaaatgaag ccgccatgca ggcctcacaa gtggtcttac
     3781 ttgattctga cttttcacgc atgccagaag ttgttgcaga aggacggcgc gttgtgaaca
     3841 atattgagcg atcagccagc ttgtttttag tgaagaatat tttctccttt ttgctctcac
     3901 tgttttcagt gatctttgct ctgacctatc ctttagaacc atcacaaatc acattgatta
     3961 gtttgtttac tatcggactt ccatctttct tgcttgctct agaagaaaac aaaaagcgaa
     4021 tcagagggaa attcatcatg aatgtgatgg aaaaagctgt gccaggtgga ctaacagata
     4081 tgatcgtagt cggtgcgttg gtgatctgcg gggtgacttt gaatctaaat aaaacagatg
     4141 tttctacagc atctaccatg cttttgattg cagtagggtt cctcgttctc tataaaatct
     4201 gttcaccgct gaataaattc agaagtcaga tcattctttt ctgtgctagc ggtaactttt
     4261 tttcggtcat ctttttgcat aagctgtttt caatcaccgg tatctcagct gtatcacttt
     4321 tactgttgag cattctattt ttctcggcag attcgatttt taggcatttg accacattgg
     4381 tagaatattt gtttgaacgt aaaaatgaaa aagagcaaaa gccgtttagt cgcgtagtta
     4441 aaaaatttct atcgatattt aaacgagaaa attgacgaaa agtatctcaa aaaagagtaa
     4501 aaaagaacaa ccactcgtgt atctttcgaa gcaggatgag tcatccaact ttaaaacgca
     4561 cgagcagttg ctctttttta cattttcgga atggtaacgg tgaaagtcga acctgttcca
     4621 agatgactgt ctacagtaat cgtaccatga aggagttgca cgtagtcttt tacaatcgct
     4681 aaccctaatc cggtcccgcc tgaatgtcgg cttcgcgcct tgtctacgcg gtaaaaacgc
     4741 tcaaagatcc gttcctgatc ctcaatgtcg atacctatac caaagtcttg gacagaaaac
     4801 gatagatcat ctgttgcctg atagcggata ataatctcgc tgtcgctttt tgaatattgg
     4861 acagcatttt caatcagatt tttgataatt ggataaagga tatctgtttt agtagtaaat
     4921 ggttcgttct ttccaatcaa tcgaatcgtc aaccgtttct tttcgatgat tggttgataa
     4981 ttttgaacga tttgctggaa ataaggttct gtataaattg tctggatctc gtaagaattc
     5041 tcgccatctt ttgacagctg gatgatttcc cgaatcagtt tatctaaccg gatcgcatct
     5101 ttttgcatga tctcaagaaa agaagtcaga gtttgcgggt catcttttgc accatccagt
     5161 aaagtctcgg taaaaccaat cagtgaagtc acaggagttt ttaattcgtg agagacgttg
     5221 ccaacgaaat ccttttgtag cttttctaaa cgtcgcactt tcgttaagtc atacgccacc
     5281 cctagaatct gtccggtacc ttctgaatta gtaaagtagc gcaaactcaa gtctaatgtc
     5341 tgccgacctt ctgtaagggt gatctcctga tggatcagcg gatgctcagg agtcacttga
     5401 tgaatcagct gaatgagttt tggctcttga atgacttctg tgtaccgctg ttcaggttga
     5461 tagcttacca cgtttaaatg tagctgcatc tttggattaa gcagtagtaa acgagaatca
     5521 gcatcatcaa tcaggaatac gccaatcatc agttcgttca gtaacgtata taattgctct
     5581 tctgtggtag tatacgcccg atacatctta ctcatttgtt cgctcaacgc attgatcgtc
     5641 tgatacaatt cttcccattg atcgggtgtt tgcatgatta cttccgcctg attaggattt
     5701 tgcaccattt tttttagtac gggcagcacg gttttcaatg gctggttttt ttgatagatc
     5761 agataataga tcattcctgc aagaacaagg aagaaagaca gaaagaagaa aaagacccaa
     5821 cggcgaaaac tctcagttct cggtaaaaaa ccgcttgtcg gttctgcgat acgtaaaatg
     5881 gcttctaact ggccactttt tttgactggt aatgcgacat ataaaagatc ctcattcaaa
     5941 gtagtacttt tgcgcaaagc gctacctagg ttcccaccat tcaatacggc tttgatttcg
     6001 ggacgggagt ttcgctgttc attcaatgtt tcattgtttg tgtcaaataa gatctttcca
     6061 gtagcatcca ttaaagtaat ccgttcgttc gactgatgga cgaattcttc caaagcagtt
     6121 ttgttttgcg ctgcttggag gttttcgaca tctaattgcc agatcagcag ttgtactttt
     6181 tttgtcaggt agctttcttg ctgttccacg atctgctgtt gaaaataatg ggaaatcaat
     6241 tgccagccac caaaaaacaa actaactaaa agaataagaa agaagagacg ttcataaatt
     6301 tttttccatt tcatcgtttt ggttcctgaa acttatagcc aaatcctcta atagtcacca
     6361 agtactttgg atgttttgga tctcgctcga ttttttctcg caaatgacta acgtgtacat
     6421 ctacgattcg gctttgtcct gcgaagtcaa aattccaaat gcggtctagc aatgtgtctc
     6481 ggtcgatcac acggtctttt cgtttcatga aataaacaag taattcaaat tctttcggtg
     6541 ttagttcaat cagatggtcg tctacggtca cttgataatt ggtcaaatca gcagtgattt
     6601 gtccaatcga tagatattca ggctcagctt catcttttga ttgcttacga ggttcaatcc
     6661 gtctgaatat tgctttcata cgggctaata cttcgcgagg actaaatggt ttggtcaaat
     6721 agtcgtcagc acctatttct aggccaatga tccgatccac ttggtcatct ttagccgtca
     6781 gcatcaaaat cggtgtttca tttttttctt gacgcagccg ctgagtgatc tccatgccat
     6841 ccatcgttgg aagcatcaca tctaaaatga tgaaatcaaa tgattgttct aatgctaatt
     6901 ctaatccttc gcctccatct gtggcactgg ttaccttgta gccttctttt tccaaattga
     6961 acgtcaagag cgttacaata gaaggttcat catcaacaac tagtattttt ttcattcaaa
     7021 tctcctttaa ataggtttca ttctgtttat attttagcat agaatcagta caaagggcta
     7081 ttttacaaaa gaaataaatg gtataatagg aaggcacttt gctgaataga aaggaagagc
     7141 agtcaatgat tggaatcagt gcatgtttag gcggtctggc ctgtcgctat gatggacaga
     7201 aaaaagaagt accagaattg atggaactag ttgaaactgg agaagctgtc atggtctgtc
     7261 cagaagtgat cggcggatta ccaattccta gagatcccgc tgaaatcatt ggtggaaatg
     7321 gattcgatgt gtggaaagat caagcaaaag tatggacgac aagcggcgaa gatgtaacgg
     7381 aagcgtacaa aaatggcgcg atcaaagcct atcaaaaatt gcaggaaaaa cacattgatc
     7441 tagtcatcat gaaggaaaaa agcccttcat gcggaaccca ttcgatttat gacgggagtt
     7501 tttccggaat aaaaaaaaat ggtgccggag tggctactgc ttattttatt cagcaaaaaa
     7561 tgactgtgct ctctgatgaa gaatggctga aaactagagg tgagcagaat ggaaatcgaa
     7621 aagaccaatc gaatgaacgc gctctttgaa ttttattcga ccttattgac agaaaaacaa
     7681 atgaactaca tggaattata ttatgcagat gatttttcat taggtgagat cgcggaagaa
     7741 tacgaagtaa gccgacaagc agtatatgac aatatcaagc ggacaagcaa gatcttggag
     7801 gattatgaaa aaaaactcca tcttttttct aattatattg ttcgagaaca ggtattggaa
     7861 aatatgactg catatattac agataagtac ccggaagata aaaaactact tgaatatgta
     7921 cagcaaattc aagcaatcga agaataaata aaaggaatga agaaaatatg gcatttgaaa
     7981 gcttaactga acgtctccag caggcgatgg gaaaaataag aaaaaaagga aaagtatcag
     8041 aagctgacgt aaaagaaatg atgcgggaaa tccgccttgc cttactagaa gccgatgtta
     8101 acttgcaagt agtcaaaaat tttactaagc gcgtacgcga acgtgcggta ggggcagaag
     8161 tgctggaaag tttatcacct gcacaacaaa tcgtaaaaat cgttgatgaa gaattgacga
     8221 tcacacttgg ttctgaaaca gcagagttaa ataaatcacc aaaaatacca actgttatca
     8281 tgatggctgg tcttcaaggg gcaggtaaaa cgacctttac tggtaaatta gccaattatt
     8341 taaagaaaaa tgaaaatgct cgtccgctcc taatcgcagg ggacgtatat cgaccagctg
     8401 cgatcgacca attgaaagta ttaggtcaac agctagacgt tcctgtattt gatatgggaa
     8461 ctgaagtcag cccggtagag atcgtccgtc aagggatgga gttagcaaaa gaaaagaaaa
     8521 acgactatgt attgatcgat acggcaggac gtctgcatat cgatgaaaca ttgatggatg
     8581 agttgaaaca aatcaaagaa ttgacgcaac caaatgagat tttgcttgtt gtcgatgcca
     8641 tgactggtca agatgccgtc aacgtagctg atagcttcaa ccagcaatta ggtattacag
     8701 gggtcgtcat cactaaactt gatggggata ctcgaggcgg ggcagcacta tcgatccgct
     8761 cagtgacagg cgcaccaatc aaattcatcg gttctggtga aaaattgact gacttagaag
     8821 tattccaccc agatcgtatg gctagccgta tccttggtat gggggatatg ctgacattga
     8881 ttgaaaaagc gcagcaagac tatgacgaga aaaaagcgga agaacttgca cagaaaatgc
     8941 gggaaaattc attcgatttc aatgacttta ttgaacagct tgatcaagtg atgggtatgg
     9001 gcccactgga agatctaatc aaaatgatcc caggaatgaa tcaagtccct ggaatcgaaa
     9061 acgtcaaggt agatccaaaa gatgttgaac gcaaaaaagc gatggtttat tctatgacgc
     9121 cagcagaaag agcaaatcct gatctgttga acccaagccg tcgtcgccga attgctgccg
     9181 gttctggaaa cagtgttgtt gaagtgaatc gaatgatcaa acaattcaag gaatcaagaa
     9241 aaatgatgca gcaaatgtcc aaaggggata tgaacatccc aggtatggac caaatgtttg
     9301 gttcaggcgt caaaggaaaa ctaggcaaaa tggcgatgaa tcgaatgatc aagaaaaata
     9361 agaagaaaaa gaaaaaacgg aaataagatg aaaaaaacag cttcggtaca gactgaaaat
     9421 gtctactgcc ggagcttttc tatcagcaat ttttattctt tactaataca attactctaa
     9481 acgaagaggt ggcacagatg agatgtccgt ggagtgaaca aacagaaagt atgcggcaat
     9541 atcatgatac tgtctggggt gttcctgaat atgatgacca aaagctgttc cgaaaattga
     9601 tgcttgatat taatcaagcc ggattgagct ggcagacgat tttaaataaa agtgaagcat
     9661 tcgatgaagc atatgatcga ttcgatatca aaaaagtcgc aaattatgga gaaacaaaaa
     9721 tcagtgaact aatggaaaac aaaggaatta ttcgtaaccg aagaaaaatc gaagcagccg
     9781 tccaaaacgc caaagcagtc ttgaaaattc aagaagaaat cggcagtttt tcagagtatc
     9841 tttggtcgtt ttctaatgga aaaatcgttg attcttctta tcattcacaa gaagaagtcc
     9901 caacaacaag tcctctttca gatgaattag caaaagatct aaagaaaaga ggatttaaat
     9961 ttatcggaag cacaacaatc tatgcttttt tagaagctgt cgggatcatc aatgatcacc
    10021 ttgacagttg tttccgcaaa gaagaggtcg tacgtatcgg cagaaagggc gaataaagca
    10081 tgcgaaaaat catattttat ggtgctataa gtttagatgg ctatttggca acgaaaacgg
    10141 atgatcttca atggcttttt gacactccaa caggtaagca aaccacttat gaagcttttt
    10201 atgaaacaat cgatacgtcc atcatgggca gaaaaactta tcaggaagca aaaaaacatt
    10261 tggagaccga agcaatttat ccagagaaaa agaatttcgt tttttccact cacgcacaat
    10321 tacaattgcc agatgctgag gtcgtaaacg aagatcctgt tgcttttcta aaaaatctac
    10381 aaatgacaga aggaaaagat atctgggttg tgggtggcgg tcgattgatc aaaccgctat
    10441 tggaacatca aatgattgat gaatggtata tccagattac ccctgtatta ttaggggacg
    10501 ggattccttt attccaaaaa ggaacatatg aagcgcgttt tgaaatgcta gacacgacac
    10561 gattcggtga atttatcgaa ttgcattatg tacgaaagta gaagtaaata aaataaaaaa
    10621 ggcaactata tgcagaaatg tcctgtacat agttgccttt tttattaccg ttatgctttt
    10681 tactctgtta atttgttaga aaaatctaga tacgcatcta aaatcgtttg gaagaaactt
    10741 aatgttcctt caacgatatt accattttca tcaattagat tcgcaacgtt tccgatatat
    10801 gcttccggtt gctgcaaggt tggcacattc aaaaagacaa gtgattggcg tagatggtgg
    10861 tttgcgccaa agccgccgat cccacctggt gatgcagtaa cgactaaacc tggtttatga
    10921 tcccaaacac tgtgtccata aggtcttgag cctacatcta aagcattttt caatactgct
    10981 ggcacagaac ggttgtattc aggagtaacg aaaacgacac cgtctagtcc cctcacttcc
    11041 tcacgaaaac gagtccattc agcaggagcc tgttctggag tttcgagatc ctcattgtaa
    11101 aatggcagat catcgatttt tacaaataca ctttcgtacc cttcaggaag caatttcgca
    11161 aaagcttctg ctactttttt gttgtaagaa tcttttctta aactaccaat aaaaaatcca
    11221 attttttttg tcatacgatt tttcctcctt caaactatct ataataatct tacaaaaaat
    11281 aagtaactgg agcaaataaa aacacttact gacaaaagct acatagaaga tagattattt
    11341 atttttaaca aaaaagaagt ctaaaatctt ctatttccta aaaaaaatcg aatttaatca
    11401 agctgtaaag aaaaaaagct ttacagggta aaaaaaaact gttacactac atcttgtgaa
    11461 taaaacaatg gaggtggaaa taaataatgg cagttaaaat ccgtttaaaa cgtatgggtt
    11521 ctaaaaagag tccgttttac cgtatcgtcg tagctgattc acgttctcct cgtgatggac
    11581 gtttcatcga aactgtaggt acttacaatc ctttgaaaga ccctgcagaa gtagttttaa
    11641 aagaagactt agttcttgac tggttatcaa aaggtgcaca accttctgac actgtacgta
    11701 acatcctttc aaaagaaggc gttatgcaaa aacaccacga agctaaattc tcaaagaaat
    11761 agggtgaccg gatatgaaag atttaagcga cttagtctta acaatcgttc gtccgctagt
    11821 cacttatcct gatcaagtca agttagacgt cgtagagtcg aaagactttt acgagtacaa
    11881 tctaacagtg gctcctgaag atattggccg tatcattgga aaacaagggc gtgttgctaa
    11941 agcaatccgc acaatcgttt acggtgttcg gatcaatgca ccgaaaaaag tgcgtttgaa
    12001 tatcatcgat gataaggaaa agtaaataaa cgtgctgtac tcgaattttc gagtgcagct
    12061 tttttgtttt ttttattaat tgaagaaatg attatttggc aaaacaagca agaaagcgta
    12121 tccttatagg aggagggagt gaacagccaa tgagaaagaa aatgttattt attccatttg
    12181 ctctattgtt aagcgcttgt agttcggtta ctcaagaatc agacaataca catctatcaa
    12241 gcagtatatc tgaaactgtt accagtctag aaaaaagtag taccaaaacg gcttccacca
    12301 ctgaacaaac aatggctacc tcaaataatg agaaaaagac tgctttagac cagttgaaag
    12361 aacaacagcc aaatgttccc atgcctctag atgtaccagt ctcttcaggc tatctcaata
    12421 tcgcagcgac acatacaaag caaggttatt ctattttata ttacagaaca gatcgtcccc
    12481 tcgggttgaa tgctgacgaa ttgaatcaag aaacgcctat tgcaacctat ctatatcaat
    12541 atgggtttgc ttctagtcaa gaaacaatcc aagttttgca gccctttgag atcgacacaa
    12601 agggtcagca agtggactta ggttctcgaa tcacagggta tcagcaaggt gcagcaggtt
    12661 caagttttct tgaatggcaa gaaggaaact ggcgtattcg aatccgtggg aataatatag
    12721 aaggccaaga tcccttacta ttagctaaag aaatcgtagc ttatttggaa gaaaacagtc
    12781 taccagcacc tgaacaattt ggtaaaatta ctgttgatat gggtgatacg acgaatcgag
    12841 ccgtagaagt aagctggcaa gaaccgaaaa atgcctatac gatcactcat caagatccta
    12901 tgtctgcgtt gaagatggca gtttctatga agaggttgtg aaatcagcgc gctgatctga
    12961 gcccatcatt taactccaag tcacaggaag taatcaaaaa tagcacgcca catttttgca
    13021 taattttggc ttaatacctt aaaagaaaac tagaattgcc tcttcacaca tgctaaaata
    13081 aaacagacaa aaaagaaatg gggatataag ttgactgaat acttgaatgt aggaaaaatc
    13141 gtaaacacac aaggtatcaa aggcgaagtc cgtgttatct caacaacgga tttcccagaa
    13201 gaacgttaca aaaaaggaac ggtgctaaca ctattccaag acggaaaagc acctgtggaa
    13261 ttaacagtca aaagtcatcg caaacataaa aattttgact tattgagttt tgaaggacat
    13321 ccttctatca atgatgtgga aaaatatcgg gatggcatat taaaagtgtc aaaagacaac
    13381 tctgtagatc tagctgaaaa tgaattctat taccatgaga tcatcggtct gcatgtatta
    13441 gaagatggaa ctgagttagg taaagtaaaa gaaatcttgt caccaggtgc caatgatgtt
    13501 tgggtcgttc aacgtccgaa aaaagctgat atattgcttc catatatcgc ttctgtcgtc
    13561 aaagaagtcg atttagaagc tggagtggtc cgtgtagaaa ttccagaagg actgattgac
    13621 gatgaagatt gatgtattga ctttatttcc aagaatgttt gaaggtccta tgggggaatc
    13681 catcatcggt aaggctgtag ataaaggatt attagatatc aatatctcta acttccgcga
    13741 ttattcagat aacaaacatc agacagtaga tgattatcca tacggcggtg gagcaggtat
    13801 gctgctcaaa gtccagccca tctatgataa catccaagcc attgaaaaag ctgcaccgga
    13861 tgtcaaaaaa agagtaattc ttcttgaccc agctggtaaa cgattcgatc agaaaatggc
    13921 agaagaattt tcacaagaag aacacttagt attcatttgt ggtcattatg agggatatga
    13981 tgaacgtatc cgcacattag tgacagatga agtttcctta ggagattatg tccttactgg
    14041 gggagaatta ggtgcgatgg tcatgatcga tgcaacagtt cggcttttgc cagatgtgtt
    14101 agggaatcag acttcagctc aaacggattc ttattcaacc ggtttattgg aacatcccca
    14161 atatactcgc ccggcagaat ataaagggat gaaagtacca gaagtattga caaatggaaa
    14221 tcataaattg attgaagaat ggcaattaaa agaatcacta agaaggacct atcaacgtcg
    14281 gcctgatctt ttggaaacac ttgaatggac acctcaaatg acgaaatttt tagaagaaat
    14341 caagaaagaa gaacaagaaa aaacagcaga tgaagccaag tgactttttc taaaatcttc
    14401 tttacagtag gttgataata tgttaatatt ttatggtgag tgcaaaagca cttaactatt
    14461 acgatattcc gctgtgagaa aagctcataa gaatatttgg aataaggaga agaaaaccat
    14521 gaatccatta atcgaagaat taacaaaaga acaattacgt tctgacatcc cagcgttccg
    14581 ccctggtgac actgtgcgtg ttcacgcaaa agttgttgaa ggtactcgtg aacgtatcca
    14641 gttatttgaa ggtgttgtta tcaaacgccg cggtgctgga atcagcgaaa cttatacagt
    14701 acgtaaagtt tctaacggag tgggcgttga acgtacattc ccattgcata caccacgtgt
    14761 tgcaaaaatc gaagtagttc gctacggtaa agtacgtcgt gcaaaattgt actacctacg
    14821 tgcattacac ggaaaagctg ctcgtatcaa agaaatccgt cgttaatcat tgttatcaga
    14881 ctgagaaaga acccttggta ttcaagggtt cttttttctt agcatgtaat ttgaacaccc
    14941 tgatccagtt tttttcttta attattggta ggggagaata atggattaga ataaatctac
    15001 ctataagtag tagtaaaaaa aagagcacta tgataatatc tttcttaaga gtaaccgctt
    15061 taataaaaaa tccagaatat tctaacataa atggagtatt cttagaatca aaaggagaaa
    15121 gaagatggtt tatacttata cggagcaaga aaaaatgaaa gttttagaca cattcatgaa
    15181 tgatggaaaa gtaaaacaag taccaagttc aacaaaaaag aaatacatcc tattgaaaga
    15241 agtcttgaaa cgctttgacc atggagtgac ttatacagaa acagaggtta attcgatact
    15301 gctgaacgta ttttctagtg gcgattatgt cgaacaaaga cgttatctaa tcacatttgg
    15361 ctttttcaaa agaagttcag acggtcgggc atatcagatg atgggaattg aaaattagaa
    15421 gttttaattt atactcattg aaaaaagttc aagtatgcga tattggttct acaccgcata
    15481 cttgaacttt ttttgtcatc tgtatctata atacaaagcc aagaacgtta ttcaaaatat
    15541 ggatagctat gataaaagct aatgagcgat tcgcagttaa ataagcaatc gtcagtaaaa
    15601 tcgttccagg agcgtacatg aaaaaatcaa ttggatgcgt tacatgaata aagataattc
    15661 tcttttattt gtacgaataa ataccaaaac gtacctccta aaattagatt ttggacagat
    15721 gacctttaaa aagataacaa gatttcgttc ttgaaacaat atatatgctt ttattccaat
    15781 gaggttgtga aaacagtatt ttgtcctgag cgttagaaaa ttgaaaatgg ggaatagcaa
    15841 ctcgtacttt accatttttt aagtgaatac aagatcagct aattgaacac cgtttattcg
    15901 tgatgaggga cacaacactg cagtcgacac atttctcttt tatgttcaaa gataacaaat
    15961 atttaaaaca aagaaaaata gtagataatc ccgtcctaac tcttttattc ttccctccta
    16021 gcttccaaat ttaagcaata cttcaattaa tcaagtttta tctttttttt tcaataatcg
    16081 taaaaaaaga gtatttttta tt
//
LOCUS       DACCKU010000071        15402 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.074, whole genome shotgun sequence.
ACCESSION   DACCKU010000071 DACCKU010000000
VERSION     DACCKU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.074"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..552)
                     /locus_tag="IVN13_002074"
     CDS             complement(<1..552)
                     /locus_tag="IVN13_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7012542.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICL"
     gene            complement(638..1597)
                     /locus_tag="IVN13_002075"
     CDS             complement(638..1597)
                     /locus_tag="IVN13_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP7012543.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(1609..3132)
                     /locus_tag="IVN13_002076"
     CDS             complement(1609..3132)
                     /locus_tag="IVN13_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7012544.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(3142..3705)
                     /locus_tag="IVN13_002077"
     CDS             complement(3142..3705)
                     /locus_tag="IVN13_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP7012545.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(3711..4574)
                     /locus_tag="IVN13_002078"
     CDS             complement(3711..4574)
                     /locus_tag="IVN13_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7012546.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(4588..5484)
                     /locus_tag="IVN13_002079"
     CDS             complement(4588..5484)
                     /locus_tag="IVN13_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7012547.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(5659..6930)
                     /locus_tag="IVN13_002080"
     CDS             complement(5659..6930)
                     /locus_tag="IVN13_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7012548.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(6920..8437)
                     /locus_tag="IVN13_002081"
     CDS             complement(6920..8437)
                     /locus_tag="IVN13_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP7012549.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            8635..9525
                     /locus_tag="IVN13_002082"
     CDS             8635..9525
                     /locus_tag="IVN13_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP7012550.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(9598..10422)
                     /gene="nadE"
                     /locus_tag="IVN13_002083"
     CDS             complement(9598..10422)
                     /gene="nadE"
                     /locus_tag="IVN13_002083"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP7012551.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(10434..11906)
                     /locus_tag="IVN13_002084"
     CDS             complement(10434..11906)
                     /locus_tag="IVN13_002084"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP7012552.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(12543..12905)
                     /locus_tag="IVN13_002085"
     CDS             complement(12543..12905)
                     /locus_tag="IVN13_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP7012553.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            13128..13880
                     /locus_tag="IVN13_002086"
     CDS             13128..13880
                     /locus_tag="IVN13_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7012554.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(14020..14943)
                     /locus_tag="IVN13_002087"
     CDS             complement(14020..14943)
                     /locus_tag="IVN13_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP7012555.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(14958..15230)
                     /locus_tag="IVN13_002088"
     CDS             complement(14958..15230)
                     /locus_tag="IVN13_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012556.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         4748 a         3157 c         2487 g         5010 t
ORIGIN      
        1 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
       61 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
      121 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
      181 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
      241 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
      301 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
      361 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
      421 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
      481 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
      541 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
      601 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
      661 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
      721 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
      781 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
      841 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
      901 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
      961 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
     1021 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
     1081 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
     1141 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
     1201 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
     1261 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
     1321 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
     1381 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
     1441 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
     1501 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
     1561 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
     1621 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
     1681 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
     1741 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
     1801 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
     1861 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
     1921 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
     1981 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
     2041 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
     2101 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
     2161 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
     2221 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
     2281 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
     2341 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
     2401 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
     2461 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
     2521 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
     2581 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
     2641 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
     2701 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
     2761 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
     2821 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
     2881 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
     2941 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
     3001 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
     3061 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
     3121 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
     3181 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
     3241 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
     3301 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
     3361 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
     3421 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
     3481 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
     3541 tcctgatccc tcacctttcc attgcgccat tacataaaaa acagcacatg tagcaggcac
     3601 gatagtaata acaggcaaac acacgattac ccagagaata cctaataaga tgtaatcaga
     3661 aaccacaatc aactgtccat aaagtttagt ctcaactatt ttcatcttcc tcactctttc
     3721 attccagaca tagctgttcc ttgaacaaaa tatttctgtg caaaaatata aaccactagt
     3781 aatggcagca ttgaaataac cgttccagcc attacaggtc cataaaaaga aatgttctgc
     3841 cctgtaaata aagctaaccc tgctggtaaa gttctcattt ttgaactact tgtcaatatc
     3901 aatggataaa gcaagtcatt ccaactattc atcagcgtga aaatccctaa tgataaaaga
     3961 gtaggtttgc tcaatggcaa catgattttc aaaaaaatat taaactcact taatccatca
     4021 atgcgtgctg cctcttccaa agaatctggt aaagtaataa aaaaggaacg catcatgaag
     4081 ataccaaatg cagttgtcat acgaggaatg atcaaacctg cataagtatc aagcatgcca
     4141 agcaaattga cttcaataaa tagaggaatc atgaagactt ggaatgggat catcattgtc
     4201 agcaaaacga aatagaacaa aactgatttt cctttgaaac gcatacgagc aaatgcatac
     4261 cccgcaagag aatcaaagaa tacagaagtg atcactgtcc ctccagcaaa aatcactgta
     4321 tttttgatgt aatctaataa agggatcgtt gtccaaacct taatatagtt atctagggta
     4381 tagctatctg caaatattct aggcggaaat gagataatgt ctttttcgtg tttaaaagaa
     4441 gaaataatca gccacaaaaa aggaaacgca ataaccaaca atataactag catactaatc
     4501 gttcgaatga tttttttacc actaactgct tttcgactat tcacagtaac tgtaatcgag
     4561 ctttctggtg ccatacaggt ttccctccta tttcatttgt ttttctttcc gtgacatgta
     4621 cacattagtg gagatagcta tagttgcaac aatgataaac aaaaccgaag acagtgctga
     4681 tgcatagccc aattcataag gtgctgtaaa tcctcgactg taaatatatt gaacagctgt
     4741 ttctgtctta aattgtgggc caccagcggt tgcaacatat acttggtcaa acacttgcat
     4801 cgaaccaatc agattcgtta aaatacaaaa acctaaggag ggaacgatct gaggtagcgt
     4861 aatgttaaaa aactgctgtc gatttgttgc accatccatt tgagcagatt catacaaaga
     4921 gggagagatt ccctgaatag cagtcaagag aatcgtcata gtaacaccaa aattcttcca
     4981 aacagtcatg atggctaccg tcggcatagc aagggttgga tcgcgaaaaa attgagggat
     5041 cggcaagccg acttgtgaaa acaagtaagg aatcgcccct acattcggat caagcaacat
     5101 agaaaaaata atcgcaattg acgtcaatga acaaataact ggaatgtaaa atgtcgaacg
     5161 acaaaaacga ttgaatctgg tatttttaga taatgctact gctgctaata aaccaacgat
     5221 aatttgtgtt ggcgtttcga gtacggtgaa atataaagaa tgaatcaacg aattaactgc
     5281 tcgttcatca ctaaatatac gtgtgaaatt ttcaaaactt acaaaatttc ttgatgtaaa
     5341 gaaaatattc aaatctgtaa aactaatagc aaatgttcct aaaagcggta tgacagtaaa
     5401 taataataaa actaaaaaag atggagttaa aaacaaatac gcagcacgat caggacgatt
     5461 ccaatatttg gtgaatccgt tcatatagtc cacctcatct ttcattttga taaaatatac
     5521 aatcttggtt taaacgaatc tgaataagat atccgaacaa tcgctgtgat ttttgcaata
     5581 acacctatct gcaaatacta ggtcgatcat tcagatatct gtatcttgcc aacagtctat
     5641 cctgtataaa gttctgtact atttttgcag taattgattg atggcatcat ctgcatcgtt
     5701 catgagtgtc tcaggatctt ctcctgctaa taatttttca atcagtggat tgatgatatc
     5761 gttattgatc gcagagattt gggtaaaacc tggtaaatat ggctctgcat agtccatttg
     5821 tttcgataat tcagatacaa taggatcatt ttttacttct tcatcttttg ctactgaatc
     5881 caagtaggca ggaaaaccat tttcgatact ccatttttta ccaatttcag ttgtattcca
     5941 atatttcaaa aactcataga cagcttcttt tttattatta tctgtacttg ccggaatggc
     6001 atatcctacg ccatctaaaa tagctgtttt tttgtctgaa tcactccctt gtggtaaggt
     6061 agtcacatta taatcaatct cgtttgcttt caatccgtta tttaaccatg gcccattgat
     6121 ttcaatggct agttgaccag cattcattaa attgtccatt tcggcgccag aaatcgattc
     6181 tggaccagtc ttgtctacat ggatcatttt ttggatcgct tttaacgttt taaggttctc
     6241 aggagacgca aattcagact tagtataatc atcgttcact aatttaccgc catttgctaa
     6301 catccagtta tataacggtg cagttccatc cttattgaag acaaaacctg atacattttt
     6361 actactatca gttaattttg gtgccatttc tgtcaattct tcccaagtag taggtggttg
     6421 ttcagtatct aatccagctt gtttaaacaa atcattattc caatacatat acatgccttg
     6481 tacctgcatg gggacaaaat actgtttacc ttctacttca cctaattcaa tagccgtttc
     6541 tttaaaatca gatttatcta ctccatcata ttcccagaaa tcgtctaatg gttgtaccgc
     6601 tccgttttct acatactgtg caaaatctgg ataatttaat gcaataaagt caggtgcttt
     6661 tttagaagaa atcgctgtag gaagtttttc attcaaattg gcccaagtca tgatatccat
     6721 ttcaattttg atgtttttat catttgtttt attaaattca ttaacgattt cttgcaatat
     6781 ttcaccatca gatgcagtaa acccattcca aaaagtcaaa gtcacttctt tatcagaatc
     6841 agattctgct gaacttccgc caccgcaagc tcctaaaata cctgttgcag ctaccaacaa
     6901 agagccaatt aaccattttt tacctttcat tttttgttcc tccttcatct gataaacgac
     6961 atctgaccac attccaagat agcggtttca ataaacaatt gattttccca tcttcagctt
     7021 ttacatttgt tgaatgactt ggttttactt gttcactttt aggaagatta gttgcttttg
     7081 tatcaaatcc actcatttca gaaaaatcaa tcactccctc taatgataat tcagacaagt
     7141 gaacagttag attcatttct tcttctccac ggttaactgc aaatatgaca agttctttgt
     7201 catttattac ggctatactt tctaaatacg gcacttcttc aaaatcttcc gtcgcatagt
     7261 ttgctgaacg aatttgtggg cttaacacct ctccttgtcc atacacagca gcttgcataa
     7321 atggataata gatcgtctgt ctccaagctt ctccgtttgt ttctgtcctg attggtgcaa
     7381 taacatttac caattgagca agacaagcaa tctttactct atctgcatgt ttcaataaag
     7441 tgattaatag acagcctaat agtaatgcat cttcaaaatt atagttatct tctagtaaag
     7501 gaggagcaat ctgccaaggc gctgcttgct catcttgttc cgctgaatga taccaaatat
     7561 tccattcatc aaaggacagg ttgatctgct tcttactatg tttcttcgcc ttgactgtat
     7621 cgcacatcgc tgtcacttca gaaatgaacc gatccatgtc taaggaacgt gcaagataat
     7681 gagaaagatc atttttttgg ttgccataat attgatgcag agacaaataa tctatttgat
     7741 cgtacgcttg atcaagaact gttaactccc aatcaccaaa tgtaggcatt tgccgattcg
     7801 aacttccgca taaaaccacc tcaattgaat catctacgag tttcatcgcc ttagccgttt
     7861 cctctgctaa ccgtccatat tcttccgctg ttttatgtcc aatctgccaa ggaccgtcca
     7921 tttcattccc taaacaccat gtcttaattc caaaagggct ctcttgacca ttcgaacgcc
     7981 ttaaatcact ccaataagtt cctttaggaa aattacaata ttcgactaaa ttacgagctg
     8041 catctactcc acgagtaccc agattgacag ccatattgat ttcggccccc acatgctgac
     8101 accagttata aaattcatga atcccaactt cattcgtttc aattgtccgc caagctaaat
     8161 ccaaccttct aggtctattt gctatgggcc caatactgtc ttcccaacga aatcctgaca
     8221 caaaatttcc accaggatat ctaatcatgg gaatgttcaa ttcttttacc aagtgaatga
     8281 cgtcttggcg aaatccactt ttattagctg atggatgatc tggctgataa atcccttcat
     8341 atactgctcg tcccatatgt tcaataaatg aactgtacaa ccgcggatca actttgccta
     8401 cacgcttgac actactaatc aacattgtgg cttccataac tgacctcctt taatttatac
     8461 atgaataata cacctcgttg aaagcgttgt caataatata aatgttaaac ttacttattt
     8521 tttatcgatt ttaattaacc aaaatattaa taatgattaa aacaaataat ttaatataac
     8581 ttttattaac aaatatgtta atatataaga aaaacctttt aagaggtgat atatatgcaa
     8641 ctacaaataa acgaagaatc cttaccggta tatgaagcat tagctagtaa aacaaggatc
     8701 aagatcatcc agctactttc caaaaaaaag atgaatgtca aagatttggc aaaagaactt
     8761 ggagtgagca gtgctatcac gacgatgcac gtgaaaaagc tagaggaagc aaacatcatc
     8821 aaaacagaaa aagtaggaca acaaaaaatt tcgagccttc gtgtcgataa aatcgatatc
     8881 agttttccag aaaaaatatt caatgctttt gatacgaaag aaacatcgat acctattggt
     8941 cactatacaa attatgcaat tgaaccaaca tgcggtttag ctacgatcca tgattttatc
     9001 gggaaagtgg atgaaccacg ctattttatg gatcctcgaa ggatggatgc ccgcattctc
     9061 tggtttacca gcggatttgt cgagtatcag gcacctaatt ttttaaatac agaggataca
     9121 ttggagatgt tggaagtttc tgtggaaatt tcttctgaat ttcctttttc aaatgacaat
     9181 tggccttcag atatcacttt cagcctaaat ggtgtggagc taggaacatg gacgagccct
     9241 ggagattttg ctgatatccg cggtaaatat acgcctgatt ggtatccaga taatcttaat
     9301 caatacggct tgctgaagac gatccgaatt accaaacact tgacgaatat gaatggagaa
     9361 cccttgtcaa acatcacgat caacgacatc cctaaagagc aagacacttg gcatttgcga
     9421 atcgaagtga aagacgatgc caaacatgtg gggggctgta ctttatttgg taaaggattc
     9481 ggtaattacg atcaggatat caaattaaaa gtttactaca gctgatattt ttcttttcta
     9541 ttttataaaa caaaaacagc caatccctat gaagggacgg ctgtttttgc tttattgtta
     9601 tttccagaaa tcatccaaaa cggaaattgg caaatgtcgt ttatgttgtg tcttgttcca
     9661 ccatgcttcg attttttctt gtgcttgagc gggaacttct tttccttcta gataatcatc
     9721 aatttcatta tatgtgacac ctagtgcgac ttcatctgca atcattggtt tcccatcttc
     9781 aagatctgca gtcggaattt ttgtgtataa tttttccggt gcattcaatg cttgaagcaa
     9841 ttgtttccct tgtcgtttgt ccaaacggaa taatggcaaa atgtcggctc caccatcacc
     9901 aaatttcgtg aagaatccag taatgttttc tgcagcatga tctgtcccta atacggctcc
     9961 tgctctttct ccagctacgg catattgcgt aatcatacgt tgacgtgctt tgatatttcc
    10021 tttgttaaaa tcgctgattc ccacacctgc ttcgcttaat acttgtactt gtgcatcaac
    10081 agctggtttg atatttactc gcagactaac atcgggctgg ataaagtcaa gagcagcttt
    10141 tgcatcttct tcatctgctt gttcaccata aggaagacga acagcgataa attgatagtt
    10201 ttcatctttt gtttcttcac gcatttcttc catcgttagt tgcgccaaac ggcctgctaa
    10261 agtagaatct tggccgccgc tgatacctag tacaaaagtt ttaagaaacg gatatttttt
    10321 caaatatgct ttcataaagt caatactttt acgtatttct tcttttggat caattgttgg
    10381 tttgacacct aattcattga taatttctgt ctgcaggctc atttattctc ctcctatgct
    10441 tctgtatgaa gttctgctac gctttttttc attcgttcca tgatagccat tttatggttc
    10501 cagcattcag tagacagatc gactgggtat ttttgcggat tcaagtctcg tttatactct
    10561 tcccatagtg aatctagatt ttcttttgta tattctttga tttcgtttag tgcaggtaat
    10621 tcgtaaactc tttttccttc gataaagata tcttgtaaaa ctgggcgagc tgtaaagtct
    10681 cgaacagttt tattgataaa cgtatgcact ggatggaaca tgaagatagc ttcttcttct
    10741 cgagggtctt catcccataa tgtcacgtag tctccttcag atttcccatc aaaatttcga
    10801 gtgatgcgcc acacttgttt tttccctgga gtagttactt tttctgcatt gcttgataac
    10861 ttgatcgtat ccatcatttt tccttcatca tcttcgatcg atactagctt gaatactgca
    10921 ccaagggctg gctgatcata ggctgtgatc aattttgtcc caacacccca tacatcaatt
    10981 ttagcttttt gcatctttaa attcaaaatc gttgcttcat ccaagtcatt cgaagcatag
    11041 atttttgctt ccgtaaatcc ggctgcatct aattgttccc gaacacgctt agatatatag
    11101 gccatatctc cactgtcgat gcgtacgcct tggaagttga ttttgtcacc taattctttc
    11161 gccacgcgaa tcgcacttgg cacacctgat ttcaacgtat cataagtgtc gactaagaac
    11221 acgcagtctt tatgggtttt agcgtaggcc ataaatgctt cataatcatt tccataagat
    11281 tggactaacg aatgagcatg ggtcccgcta gctggtatcc caaatatctt cccagcacgt
    11341 acattgcttg ttgcatctgc tccgccgata tatgccgctc tagttcccca gactgctgca
    11401 tccagttctt gtgcccgtct agttccaaat tccaatagtg gatcttctcc gatgacagat
    11461 ttgatacgag ctgcttttgt cgcgatcaac gtttggaaat tgaccatatt caacagtgct
    11521 gtttctacta attggcagtg cgctaaaggc ccttccactt ggattaatgg ttcatttgca
    11581 aaaactagtt ctccttcacg agcagaacga actgttgctt taaattcaaa attttgtaaa
    11641 tactccaaaa aaccttcagg atagacttct aatccacgca agtaagcaat atcggaatca
    11701 ttgaacgtca gattctctaa ataattgact aaccgttcca atcctgcaaa aaccgcgtat
    11761 ccgtggttaa aaggcatttc tcgaaagtaa cattcaaaaa cagcatgtaa atcagctcga
    11821 cctaattccc agtatgtctg catcatattg atttgatata gatccgtatg cagcgtcaaa
    11881 ctgtcgtctg ggtaagtatt attcatcttg tattactcct cttggcaagt tttcatttcc
    11941 tacccattat aacaaaaaaa attaatagga aaagaaatct tccatttttt ctgaaaaagc
    12001 acatgtttta cggatatttc ttgctaaaaa tgaactccat ctgctaaaac tatcctttct
    12061 tattggtatt taacctaaac gagaaagaat acttatttta ttcaatgtat ccgagacacg
    12121 aagaaattag cgttttctat ttttcttact tgataaaata agtagatttt cctactacaa
    12181 tcgtagccgt taagataccg atgattgaac aaatataacc aaatgtctct acaacataat
    12241 caatggcaaa agtttttgaa gcaataatac gtctttcctt ttttgcttct tatccccaaa
    12301 gaaacggata taacttatta tgtattgaat ttttttggaa aggcgaatta tgacaagata
    12361 taccttgcgt tgaaaaaact cgcggaaaaa atatcacacg tttttaaaaa gatacaaagt
    12421 aagaaagcag ctgtcaatag aatggaaaca aaaatggcga gacatcaatc agtctcgcct
    12481 tgttttttat tgtatacatt agatatggag caggttacca taaagatgat tattagtcgt
    12541 tattaaatag aatccttcgt atttttttgt tctatcacta caatatcttt atttggacgt
    12601 ttgaattgta ttccggcacg ttctagctcc tgtttcactc ctacatggac tttgacatct
    12661 tctcgtgtca tttttaaaac gtgatgaatc gcatagtcga tttcttgcct tgtgattttt
    12721 ttcttcgtat tgatcatcac gacttgcttt ttatcatatg tgtctaccaa tagcagttgt
    12781 gttcgatcta acgaaaataa tttgtaataa ttaatagccg tattcccaaa aaagaattcc
    12841 actactttag gataaaatgt cttaatattt aaattactat tcacaggtgt ttcgattagc
    12901 ttcatctcat tcctcttttc tatatagtcg atttagatcc aatcttaagt tatgttacca
    12961 tctttcctag cattaaggaa tcggttacaa ttgaaccttt aaagacaaaa ggatttacct
    13021 tttatcactt aatagtataa cataatttcg tgagaaaaaa atacaatttt ttcttgacca
    13081 tctcgttacg taaaggatta tgcttacatt tgtcaggagg aaaaataatg gagtacacaa
    13141 taaagaaaat gtcagagata tcaggagtca gtccgagaac cttgcgcttt tatgatgaga
    13201 ttggactgct aaaaccagct aggatcaatt cttcaggtta tcggatttat ggcaaaaaag
    13261 aagtcgatcg tctgcaacac atcttatttt atcgaacaat ggcattcaag ttggatgata
    13321 tccaagaggt cttagacaat ccatcttttg accaccagaa agctttaatc aagcatcagc
    13381 aaatgttgct ggaaaaacgg gcacaaatcg ataccctctt gacaactgtc caacagacac
    13441 ttgatatgta cgaaggagga agaaaaatga gtgacaaaga aaaatttgaa gggtttaaac
    13501 aacaaaagtt gaaagaaaat gaagaatcct atggacaaga gattcgacaa aaatacggaa
    13561 aaaaaaaggt tacagcatcc aatgaaaaat ggatgaatat ggataaagaa acgtatgaag
    13621 aaatgcaaac tgttgaaaag cagatgttat ttgatctcac tctttactta aaagaaccag
    13681 aagatgaaag cttagcagac cgtatcttcc aagctcataa gaagtggttc tcttacagct
    13741 ggcctgaata tcagccaaaa gcgcataaag gactaggaat gatgtacgaa gccgatgaac
    13801 ggttcactgc atattatgac gagcgaagtg gtcatggtgc agcaaaagct ttaaatgaaa
    13861 ttatccaaaa aaaggcgtaa tcttttacat gatccaacaa aaaagtacac gtaaaatgac
    13921 ctaataacaa aacagcagac acccaagtta gatttttatg ctaacttttg gtgtctgctc
    13981 cattctttca caagtttttt catcctactt taaatatatt catcgttgaa taaacgtata
    14041 aggtattttc actttatcct ttttttctgt aagaaacaat tgaaatgcca tttctccgca
    14101 agcttccaga tggtggtcga tggttgggaa accaagaagc tcgctgatca gcaaattatc
    14161 gcgtccaatc accactggtg tcttatcttg cagatagctt tgcaagatgc ctgctgcaac
    14221 catatccgaa tttgtaaaga caccatcaac tccaagctct ttaaaaaaga agcctgcttg
    14281 ttttcctttc tctgcatcta tacaatccca aaaaacaagt gattcatcaa aagatggaaa
    14341 aagttgatga catagctgca aagtgatttt tgagttataa cttagcctgc cacttcgccc
    14401 caacgtgatg cccaattgct ttataccttg ctgcttcaaa taagtaagac cttctgtaat
    14461 tgattgttct cgatcaataa agacacagct tgcctgtttt cccttgattt cttcacaaaa
    14521 aactagaggt ccatattttt gatatgccaa taaatcatct agtgtattgg cacgagacgt
    14581 aatgatcaat ccatcgaaag ctttagctgc aaattcttca agataccttc gctctttttc
    14641 tttatgatag cccgtcggta aaagtgtcac tttgtaatca taatcaaacg ctgcttcaat
    14701 gatcccactg atcaactgat caaaatacgg atgattggta taaggcagaa tcactccaat
    14761 gcttttcgtt tttccataac ttaactgtct ggcatttgca ttagggatat aatcgacttc
    14821 tttcatgacc gcaagtattt ctttcctttt ttcctcatct acatatggat gtccattgat
    14881 tacacgtgaa atagtagaaa ctgaataacc tgtgagcttt gcgatatctc gaatgtttgc
    14941 cactgctgct tcgccaccta tacaaaaaga ttttcttgta aatagtctac cacagctgga
    15001 ccgcattcaa aaaactgata catttttcct tcatgcgaaa aactgatccg ctgcccgccc
    15061 caatatatat cttttgtcaa attttcgatt tcattcattg gtatttcttt tactatattc
    15121 ctattcaacc cttgaaaact gtacaacgtt aatacggatc ccttgaactc gacaaacgca
    15181 taaaaataat tgttcgttgt gccatctgtt atctctacat aagtattcat tgcaagccct
    15241 ccttttgata ctttgagtat agtgtatgaa acgcgtctca tagcaaggag tttgttttgc
    15301 caaagaaaag ttttattcct ctttcattat taccaaagtc tcttatttta aattgaatac
    15361 attataatga tactgtaaat atatctttat tattagtgaa tg
//
LOCUS       DACCKU010000072        15291 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.075, whole genome shotgun sequence.
ACCESSION   DACCKU010000072 DACCKU010000000
VERSION     DACCKU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 15291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.075"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(93..2192)
                     /locus_tag="IVN13_002089"
     CDS             complement(93..2192)
                     /locus_tag="IVN13_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP7012557.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(2412..3521)
                     /gene="rpoD"
                     /locus_tag="IVN13_002090"
     CDS             complement(2412..3521)
                     /gene="rpoD"
                     /locus_tag="IVN13_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP7012558.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(3545..5386)
                     /locus_tag="IVN13_002091"
     CDS             complement(3545..5386)
                     /locus_tag="IVN13_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP7012559.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(5568..7961)
                     /locus_tag="IVN13_002092"
     CDS             complement(5568..7961)
                     /locus_tag="IVN13_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7012560.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(8068..8928)
                     /locus_tag="IVN13_002093"
     CDS             complement(8068..8928)
                     /locus_tag="IVN13_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP7012561.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(8987..9617)
                     /locus_tag="IVN13_002094"
                     /pseudo
     CDS             complement(8987..9617)
                     /locus_tag="IVN13_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
     gene            9826..10545
                     /locus_tag="IVN13_002095"
     CDS             9826..10545
                     /locus_tag="IVN13_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7012562.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            10571..13162
                     /gene="mprF"
                     /locus_tag="IVN13_002096"
     CDS             10571..13162
                     /gene="mprF"
                     /locus_tag="IVN13_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP7012563.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(13350..14537)
                     /gene="tuf"
                     /locus_tag="IVN13_002097"
     CDS             complement(13350..14537)
                     /gene="tuf"
                     /locus_tag="IVN13_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP7012564.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            14905..15261
                     /locus_tag="IVN13_002098"
     CDS             14905..15261
                     /locus_tag="IVN13_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP7012565.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         4184 a         3184 c         2706 g         5217 t
ORIGIN      
        1 aaaaggagta tgacaacagt tgtgtcatac tccccttttt cgaataaacg gtgttaaacg
       61 gcaggctcac agcctctttc atttgcctaa aattaattgc tcccatttcc tccgaaccat
      121 ccagttttac agttgatggt tacgatgtaa aatggcaaag cactttctcc aatgcctttc
      181 atcaatgcag attttgttga agtggcatat agattataat agccttcccc attttcgatt
      241 ctggcttttt ctaatctgta acctccttca acgatatatc cacgttggat acatgtagca
      301 atcactttgc tttggacacc tggtgcccac gtccatgctg gattagatgc atcggcaata
      361 tcacttgctc tggcagattt gattggctga ttcgctgagt ttgaagagga aggggtcgag
      421 gatgccgctt cttgctgtgc tgcttgtgcg accgcagctt gttgagcggc ttgagctgca
      481 gctgctgctt gagcggcttg ttttgcttgt tcttcagctg cttttttcgc ttcttctgct
      541 tttttctttt cctcttcttc ttttttctct ttatcagtga gcatcttatc tacttgtttc
      601 aattgttcat tcaactgagc agatagatct gtatttttca ctttttctac tgcttctttt
      661 gccgtcttgt attggtcgcg tgttgctgaa tccactactt ttccgtcttt aaagactata
      721 gctgtttttt ctttagctgt ttgcaattca gcatattgat ttttagcttc tgtttgtgct
      781 tgttgaatca attttccaaa atctgtgtta tccgtttcga ttgcttggat ctctttatct
      841 gctttcaact tgatgtcatc tgctagatga tcatcgacga tggcaggtgc tatgaaccac
      901 tcattcactt tttggatatc tcggatctta gattggatat cttggtaagt tgtttctaac
      961 tctgaatatc ctttttcatt cttaagactg gataaatcat tttttagttt tgataaatct
     1021 tgatgaatca tatctgcacg aataaattga tggtgatcat ctgtataaaa ggcagcaagt
     1081 ttctcatgaa gttcttccat ttgttgctgt ctttttgctt ctgctgctac ttcggcttct
     1141 actttttgat gatggtcata ataagcccag cttcctccac cgagtagaag tactgctgct
     1201 aaaacgagat aaggtacttt ttttgatttt ttcttttgcg ctttatctgg atctgttttt
     1261 tctaagtttg attcttgatg tgttttttca gtatctggca cgattgttat tgatttcgct
     1321 tctgcttcaa acagcttagg ctggttctct tctttttcta tttttgcttc aagatcagta
     1381 ccctctttgc ttacagattg attttcagca tcggattcac tatttatttc atcgggctct
     1441 gttgactttt tgtctgtgtt ttcgagtatt tcttttttct ctgcttccgg cagtttattc
     1501 gttttttcct tttctaattg tttttcagta aatacttcag ttttttctgt gatatccgtt
     1561 tttacgtctt ttatttttac tgtttcttta cctgtggcgt tttctctatc agagtctttt
     1621 tcagtttctt gttccttttc tggctctact ttgttttcct gtgcaatttt ttcactgatc
     1681 attttgagca gctctttcgc gtgctcttct tttgtatctt cacgatgtgc tttgatataa
     1741 gcggatagta tcgaattttc ttcctcatta tcgacgattt ctttcagttc agctactctt
     1801 cttttttgtt ttgattcaag cgtttcttct tgattctttc ctgattcttc cgaagtctca
     1861 tgaacagttg ctttcttctg ttcatttttc tctatattag ttacttgatc gtgagcaggt
     1921 ggttcttcgt cttctggttc tgctttttcg aataattcca ttacagattc tagaggtaaa
     1981 tctttcaatt ccgaccattc gattggatcg tttaattctg tatcaggaaa gactgcatct
     2041 tcagttgatt tatcctgcat agctgcatct gtatttgttt cttcaattga ttcttttatt
     2101 tgctgatctt ctgcagttat ttgatgaccg cagtctggac agatagtatt ctgctccatt
     2161 tttttcgatc cgcaatttgg acattttttc atcacttagc gatcggctcc tttactaggt
     2221 ttacgctacc atttaatcta tcatattcta tacgtcacgc caatagctta tttttaggat
     2281 ttacttaaaa ataatgaatg aatttccttc ctatagaaaa aaaagcttga gactgcgtag
     2341 tctcaagctt tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca
     2401 atagtccacg cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat
     2461 tttcttaatg ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc
     2521 cctacttctt ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca
     2581 ttctcttccc gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca
     2641 taagctgcgt gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga
     2701 gagtcatctt cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag
     2761 atttctcgga ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt
     2821 tctcttccaa ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca
     2881 accatatgaa ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct
     2941 tgacgaatcc accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt
     3001 tcaactgcct tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct
     3061 cgtcctacat aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc
     3121 tgtttcgctt cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca
     3181 gttaaaaggg agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt
     3241 acaccggttg gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc
     3301 ttcaagctgt gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca
     3361 atcttttgga tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga
     3421 ttggataaat catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct
     3481 actgctgctt catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga
     3541 gtagctattt ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa
     3601 gctcaagctg tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta
     3661 tttcatcagc taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg
     3721 aactttcttc tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat
     3781 cttgcaaaaa gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat
     3841 cgaataaaaa ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta
     3901 aaatattacg gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt
     3961 ctacttgcgt caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt
     4021 gactttcctg aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt
     4081 tttgtatcgt ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt
     4141 gttcgattgg tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct
     4201 tgtctttttc attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga
     4261 ctgtttcgcg cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat
     4321 attcatcagg atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt
     4381 gccgaagcaa ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc
     4441 aaaagaccaa ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg
     4501 tccccataga agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa
     4561 acccttcgaa caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat
     4621 taaataaaac ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct
     4681 gatctttttc ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc
     4741 cttgagaatt acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat
     4801 catcatgcgt gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct
     4861 tgaatacttg ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa
     4921 ttaattctaa agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg
     4981 tatgcaatag catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc
     5041 ccgtagcaga ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg
     5101 gtatctgttc gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt
     5161 cttgaagaaa agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct
     5221 tgtcttctgc aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt
     5281 aatttttacc agactttttc aactggacat attgtccaat cacctcaacg atattcgttt
     5341 gactgcgtac tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg
     5401 ctctattgat atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat
     5461 cgcactaact gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca
     5521 tatttcattg ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact
     5581 cttttcagta actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt
     5641 acgagcatga atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa
     5701 attccatgga acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag
     5761 atgcgcaatg ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc
     5821 agcaagtaat acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt
     5881 agatacaccg caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt
     5941 gctgtaggca aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag
     6001 gaaggatacc cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat
     6061 ccaattgctg taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa
     6121 aagatgcctg agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct
     6181 ggcaatgtag taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca
     6241 tcaccttctg ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca
     6301 ccgtcgcctg tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc
     6361 ttttgctgcg gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc
     6421 atttcttcag taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt
     6481 ctagcgatat tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct
     6541 tcctcatgaa gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca
     6601 aacagagcaa ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg
     6661 atccccaaca taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc
     6721 cgatcttctt ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat
     6781 tccatcgcgc cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga
     6841 ttagaaagct caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct
     6901 gccagatacg tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct
     6961 acccgctgga tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta
     7021 tcgacatggg ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc
     7081 agtttggtta cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc
     7141 atgcctaaca aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg
     7201 aacagtgctt ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg
     7261 gatactttgc gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg
     7321 ctagtcaagc ggacagcata attatctgca cctactcgga taacttcagc catgacagaa
     7381 ccgcttgtca aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca
     7441 gattcgcctg tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg
     7501 gctggtatct gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta
     7561 gaaatcgatt gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat
     7621 aatttccgta caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg
     7681 acaataattg ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca
     7741 ataccaaagt tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta
     7801 ctttttgatg catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc
     7861 tcttgctgtg taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata
     7921 atcacgtgtt taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta
     7981 tagaaaaaca cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg
     8041 tgtgctgttt ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca
     8101 ccaacctagt gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc
     8161 caatcccgcg taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat
     8221 gaaatggaat agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc
     8281 aagaacacaa agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt
     8341 aaatccgtca gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa
     8401 tcctgggacg atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt
     8461 ctgatcgact tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac
     8521 aaaagtaaca accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt
     8581 ccataacgca ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc
     8641 atacgcacct aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc
     8701 tagaccaaca ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa
     8761 aaaagaaatc agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc
     8821 tggcaagata aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt
     8881 ggttgtgtac cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact
     8941 ccttctttca tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt
     9001 tttcataggt ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac
     9061 ctccattatg atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt
     9121 attgtttccc ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg
     9181 ctaattctac tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa
     9241 aattatactt ggacagctgt ccataaatcg catccttgtt tctttgcttc tactatcatt
     9301 tccgccaaat atttgacacc ttgatcaata ctgtcagctg gattatcaat gatctcagct
     9361 tctccatgca gactttccga gctctgcatg agatctgtcc cttttccttt tgattcggtc
     9421 aatattatcg ccttgatcaa attctcgtag ttttccatat gataattttt tgcagctgtt
     9481 gttatttcta tttgatagct ttccactcgt tttaccgaac gataagttct tcctcctaaa
     9541 aacaacaccg caccaataac cgtcagaatg atcatgtaaa aaagaatacg gataatagat
     9601 aatcgttttc gcttcatttc atcacctagt tattttgaca tcttttttta tcttactatt
     9661 tctttatctt cttggctatt tcttccttga tttctgtgta cgccttaaga aaaaatgaat
     9721 tttcctgtat tttttgacat cttacgaaaa tgatcgttgt ttttcagtaa aaacaattgc
     9781 ccaatgactg atataatcgt tatactttac acagtaggtg attatgtgaa aaaagaaaag
     9841 aaatatgcaa aaatgccaga tgggagcgaa atttactatg aaaagagcgg tcaaggcttc
     9901 cctctctttt tactgcatgg caatgacggc agcggccgct ttttttcaga acaggtccct
     9961 gtactcgaac gctactatac cgtgtatcta gtagatagcc gaggacatgg tcgttcgaca
    10021 aacgaagcat ctatgttgaa ttttcagtta atggccgagg atctgaatac gatcatgttg
    10081 ctggaaaaaa tcgatcaggc agattttctt ggatttagtg atggagcaaa tctagcactg
    10141 gtatttgcca gcagcttccc taaaaaagtt catcgattga ttttaaattc tggaaacacg
    10201 cttgttaaag gcgttcgttt ttctgccaga gtcatcagta atattcatta tgcatgggta
    10261 tggttactct ctttatttcg cccaagtctt cgcaaaaatc ttttagttat caaacttttg
    10321 ctgcatgaca tcgggcttac agaaaatgat ttaaaaaaaa tcaactcccc cactttgatc
    10381 atcgtgggaa aaaaagatgt aatcaagcta aaacattctt tgtatatagc taaaactata
    10441 ccaaaagctt cctttgtgct tgtgaaagaa caaggacatg aattagctag aaaagatcct
    10501 gaacgcttca atcgagaagt tcttcaattt ctttctgaaa cataactatt aggaatagaa
    10561 ggtgatttat ttgttaaaaa ataccataca atggctgaaa aaccacctag gactgttcaa
    10621 aacaatcttt ttgatttctg tcattgtcat tattgtcggt gaattgatgt ccatcggtaa
    10681 aactctatct atccagcaat taggagaaac gtttgccact atacctgtat ggaaaagcgg
    10741 actaatgcta ttgattggtc ttatctctgt gttacctatg gttggttatg atattatttt
    10801 gaatcggatg cttgaacaaa agcagaatcc tcgttacctg tttgagacaa gctggctgat
    10861 caatacgatc aacaatattg ccggtttcgg cgggtttgtt agtgtggggc ttcgctctga
    10921 actatatgga caaaagaaag agagcagaaa cgtgatccaa gcattatcga aagttttctt
    10981 gtttttgatg gctggacttt ctatttacag tctgctttct tttttacttg tagtcttcac
    11041 gccagttgct ccttttttga agcaatattg gatatggttg atcggcggcg gtctatattt
    11101 tcctgtcgtc tttttattta ctactttcaa aaagaaagga ttccttggtg gtacctcact
    11161 taaaactcaa ggacagctat tgctagtttc attgttggaa tggaccggtg tcctgtttag
    11221 tttcctttct gttggttatt taatggaaat ccggattgat ctgtggcaaa caattccatt
    11281 attcattgct gcttcagtga taggaatcgt gtctatgatt ccaggagaaa ttgggagttt
    11341 cgatgtaatg atgattatcg ggttgtccgc tattggtgtg ccaagagaaa tagtcgttgt
    11401 gtggatcttg ctctatcgcc tgttctacta catcattccg tttttgattg gaatcgtctt
    11461 tttcttcaaa aatatcggtt ccacttttga tcagcgttat tctggtatcc ctaaacaatt
    11521 agcaacagaa attgcgcata aaatcgtggt tgtcttatta tacttttctg gaatcatgct
    11581 agtactttct gccactatcc cgcaagcttt tacagagttt cgctggcttc atagtttgaa
    11641 cccattgaaa tttcatttta ttatccaatt tcctagtata ctattgggat ttcttttaat
    11701 cattatgggc agagggatcg cagcacgtgt gaaacgagca tatttgccta caatcttctt
    11761 gattgttcta gctttacttt atgttttatt aagtgatttt agtttcacac cggtggtttt
    11821 cttgtctatt ctgcttctga tcattcttgc ttctaaaaac gaattatttc gggaacaatt
    11881 gatctattca tgggagtggc gtacgatcga tggaatcatt attggtgcac tgtccttgct
    11941 ttacatcgtt attggggtat acaatctgcc agatttccct catcggcgtc accattttat
    12001 ttcctttttc ttatttcctt ctgaaaagat ttggttttcg ggattgttgg ccatcatcgc
    12061 tgttagtttc atgatcgttc tatttgttca tttccttcaa ggagaaaaga aacagattgg
    12121 cgaagccttt aatgaagaaa aggccctgaa gatccttaca acttatggag gaaactccga
    12181 tagtcaattg atcttcttaa aagataagcg gatgtttgcc tatgaaaaag acggtgaacc
    12241 aactgttctt ctccaattcg cttgcttcaa taataaatgt attgtaatgg gtgatccttc
    12301 tggtaagaaa gaagattttc cagaagcaat cgaagctttt atcgaagaaa cagatcgact
    12361 atgctattta ccagttttct acgaaacaag cgaagagatt gttatgattt tgcatgaatt
    12421 cggctacgat ttcattaaga tgggggaaga agcttatgta gacctgaata gttttacaac
    12481 ttcagggaaa aaaatgaagg gcactcgtgc tgtcttgaat cggatcgaac gagaaggatt
    12541 tacatttgat gtcttacaac caccattttc tactgagcaa atgagtactt tcaaaaatat
    12601 ctctgataat tggttgggtt cacgaaagga aaaagggttt tctttaggct ttttctctga
    12661 agattatcta caaagagcgc ctattgcagt tgtcaaaaat acccaagaag aaattgtcgc
    12721 ttttgcaacg atcatgccta cttacacgaa caataaagta ggaacgattg atctgatgcg
    12781 atatgattct aaaaaagcac cttctggcag tatggatttc ttgttcttgc atttgtttgc
    12841 ctatatgaaa gaagaacata tccaatggtt caatttaggg atggcccctc tttcaaatgt
    12901 cggaacttcc agaaagagtt ttttacagga aaggattgca tacttggttt acgagtttgg
    12961 ttctcatttt tattctttcc atggattaag agaatataaa agcaagtacg ctacaaactg
    13021 ggtctcacgt tatactctat attctagaga tagttggatt gcatatgtta tgattgcttt
    13081 gctgattatt gataatgcgc cagttgagca gacacaaggt agaataagtg gtttgaaaaa
    13141 atggatacga agaaagtatt aaaaactgca taccgataga tgaacactct catgatgaat
    13201 aaaagagact gaagtaaaac gaaagttcat gcttctacaa tctcttctta tacaattgaa
    13261 gaacaaagag cggtccaaac agaaatcctg tttggaccgc tcttttattt taaacatttt
    13321 tcattaccaa aatattcgag tcgcgtttct tatgcttcga tctctgtaac gatacctgaa
    13381 cctactgtcc gtccgccttc acgaattgag aatgtcgttc cgttttcaac agcgattgga
    13441 tggatcaagt caacatcaat cgtcacgttg tcaccaggca tgaccatttc tgtatcttct
    13501 ggcaatgtga ttgttcctgt aacatcagtt gtgcggaagt agaactgtgg acgatagttg
    13561 ttgaagaatg gagtatgacg tccaccttct tcttttgtca acacgtacac ttctgctttg
    13621 aattttgtat gtggagtaat ggaacctggt ttagcaagta cttgtccacg ttcgatatcg
    13681 tcacgttgga tcccccgtaa caacacgcct acgttatccc cagcttcccc gtaatctaac
    13741 gttttacgga acatttctac acctgttaca actgcttttt gtgtttcagg tttgatccct
    13801 acgatctcta cctcatcacc gacacgaaca gcaccacgat caatacgacc tgaggcaaca
    13861 gttcctcgac ctgtaattga gaagacgtct tccactggca atagtaatgg tttatctgta
    13921 tcacgttctg gtgttgggat atattcatct actgtatcca tcaattccat aatagcagct
    13981 tcagcatctg gatcgccttg caatgctttt aatgcggaac ctttgatcac aggagtgtca
    14041 tcgcctggga aaccatattc gctcaataac tcgcgaactt ccatttctac caaatcgatc
    14101 aattcttcat catcgactaa atcaactttg ttcaagaaaa caatcaggta ttttacacca
    14161 acttggcgtg acaataaaat gtgttcacgt gtttgaggca tcggaccgtc agttgcagaa
    14221 acaactaaga ttgcaccatc catttgtgct gcccctgtaa tcatgttttt cacatagtcc
    14281 gcgtgtcctg gtgcatcaat atgcgcgtag tggcgttttt ctgtttcata ttctacgtgt
    14341 gcggtattga tcgtaatccc acgttcacgt tcttctggag ctgcatcaat actagcatag
    14401 tcttgaggat ttgctaggcc ttttttgcct aatactgtag tgatcgcagc agttaaagta
    14461 gttttcccat ggtcaacgtg tccaatcgta ccaatgttaa cgtgtggttt acttctgtca
    14521 taatgttctt ttgccataat ttacaaacct cctaaataat atagatcact gaaaaatttc
    14581 agctctcacc tttttattat agtctttttt tgactaaatc aaaaagaata cgtccgactt
    14641 actttttgta agattcgaac taagcttatt atacgtcaat attggtcaaa gtcaactaag
    14701 agcactcgtc tgtcttatac gcgaaaaaag taccttttat aaataaaaaa atgtgttttt
    14761 tgagaaaaac ttcaagatag tccttaactt tcttttgaag caataagaac aaaatgagga
    14821 aaagtttaaa attcagctgt tgccaacaac tttcaataga cgaaatcttc tatttcgtta
    14881 ataataagga aagaggtgaa gtacatgaaa aaattaattg gcgtacttgt cgttttaggc
    14941 atatttctcg gcggaagttt tgcagcttat cattattttt acggcggcga agcttattat
    15001 acaaaaatca cgacgaatgg cgaaatgtca gctacccaag cagataacgg agagaaattc
    15061 acgacttata cctacaagca agcggcttat aataaagatg gtgaaaaaaa acaggtgacg
    15121 cttcgcgaag aaagagaaca tccattaaag atgaatgcgt atcttaaact caaagtcaat
    15181 cctcgtaaag gtgtattaag ctgggaagaa ataaaggctt ctgaagtgcc taaaaatgcg
    15241 gcagaaaaaa tcgataatta gtaaatggtc ttaaaatttt attagataaa a
//
LOCUS       DACCKU010000073        14967 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.076, whole genome shotgun sequence.
ACCESSION   DACCKU010000073 DACCKU010000000
VERSION     DACCKU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.076"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1421
                     /gene="lsa"
                     /locus_tag="IVN13_002099"
     CDS             <1..1421
                     /gene="lsa"
                     /locus_tag="IVN13_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7012566.1"
                     /translation="DTQWKLGLIGRNGRGKTTLLNILQNKLPYQGQVIHQQEFAYFPQ
                     QTKDKERLTYYVLNDITDFEIWEIERELQLMQTDPEILWREFSTLSGGEKTKVLLALL
                     FVDDTHFPLIDEPTNHLDISGRKQVAAYLKKKKQGFIVVSHDRGFIDEVVDHVLAIEK
                     SQLELYQGNFSIYEEQKKLRDEFEMAQNEKLKKEVSRLKKTAAEKAEWSRSREGDKTK
                     KQVGFIDTESRRVNKGAVGADAARTMKRSKAIVNRMETQISEKEKLLKDIEYIDSLTM
                     NSQASHHKRLLSVEDLQLGYENLLFEPIHFTIEPHQRVAISGPNGAGKSSIIHYLLGA
                     FNGKVIGEKYQPKHLSISYASQNYEDNRGTLAEFAEKNQVDYQAFLNNLRKLGMERDV
                     FHNKIEQMSMGQRKKVELAKSLSQPAELYTWDEPLNYLDVFNQEQLEQLILNVKPAML
                     LVEHDQTFLDKVSTEIISLERI"
     gene            1488..2015
                     /locus_tag="IVN13_002100"
     CDS             1488..2015
                     /locus_tag="IVN13_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7012567.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2226..3287
                     /locus_tag="IVN13_002101"
     CDS             2226..3287
                     /locus_tag="IVN13_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7012568.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3290..4186
                     /gene="murQ"
                     /locus_tag="IVN13_002102"
     CDS             3290..4186
                     /gene="murQ"
                     /locus_tag="IVN13_002102"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7012569.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4200..6182
                     /locus_tag="IVN13_002103"
     CDS             4200..6182
                     /locus_tag="IVN13_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7012570.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6247..6693)
                     /gene="nrdI"
                     /locus_tag="IVN13_002104"
     CDS             complement(6247..6693)
                     /gene="nrdI"
                     /locus_tag="IVN13_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP7012571.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            6904..8328
                     /locus_tag="IVN13_002105"
     CDS             6904..8328
                     /locus_tag="IVN13_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP7012572.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(8509..9282)
                     /locus_tag="IVN13_002106"
     CDS             complement(8509..9282)
                     /locus_tag="IVN13_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7012573.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(9279..10184)
                     /locus_tag="IVN13_002107"
     CDS             complement(9279..10184)
                     /locus_tag="IVN13_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7012574.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(10338..10658)
                     /locus_tag="IVN13_002108"
     CDS             complement(10338..10658)
                     /locus_tag="IVN13_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP7012575.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            11132..11818
                     /locus_tag="IVN13_002109"
     CDS             11132..11818
                     /locus_tag="IVN13_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP7012576.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            11820..12764
                     /locus_tag="IVN13_002110"
     CDS             11820..12764
                     /locus_tag="IVN13_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP7012577.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            12963..13607
                     /gene="pcp"
                     /locus_tag="IVN13_002111"
     CDS             12963..13607
                     /gene="pcp"
                     /locus_tag="IVN13_002111"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP7012578.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            13686..14963
                     /locus_tag="IVN13_002112"
     CDS             13686..14963
                     /locus_tag="IVN13_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012579.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         4812 a         2643 c         3214 g         4298 t
ORIGIN      
        1 ttgacacaca atggaaacta ggacttatcg gacgaaacgg tcgaggaaag acaactttac
       61 tgaatattct acaaaacaaa ctaccttatc aagggcaagt aatccatcag caagaatttg
      121 cctatttccc gcaacagaca aaagataaag aacgtttaac ctattacgtg ttaaatgata
      181 ttacggattt tgagatatgg gaaatcgaaa gagagctcca attgatgcaa acagatcctg
      241 aaatcttatg gagagaattc agcacactat cggggggaga gaagacaaaa gtcctactgg
      301 cacttttatt tgtggatgac actcatttcc cgttaatcga tgaaccaacg aatcatttgg
      361 atatctctgg tagaaaacaa gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg
      421 tggtcagcca tgaccgggga tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa
      481 aaagtcaact ggaactttat caagggaatt tctctatcta tgaagaacag aaaaaacttc
      541 gtgatgaatt tgaaatggct caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga
      601 aaacagcagc tgaaaaagcc gaatggtctc gttcccgaga aggagataaa acaaagaaac
      661 aagtcggatt catcgatact gaatctagac gagtgaataa aggagcagtg ggtgctgatg
      721 ctgcacggac gatgaaacga tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg
      781 agaaagaaaa actattaaaa gatatcgaat atatcgattc tttgacgatg aatagccaag
      841 cgtctcacca taagcgactt ttaagcgtag aagatcttca attagggtat gaaaatctgt
      901 tattcgagcc aattcatttt acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta
      961 acggtgcagg aaagtcatcc attatccatt atcttctggg ggcattcaac ggcaaggtta
     1021 taggagaaaa ataccagcca aaacatctga gcattagtta tgcaagccaa aattatgaag
     1081 acaatcgagg aacgttggcg gaatttgcag agaaaaacca agtagactac caagcatttt
     1141 tgaacaacct ccgaaagctt gggatggaaa gagatgtttt tcataacaag atcgagcaga
     1201 tgagtatggg ccaacggaaa aaagtggaat tggctaaatc tttatcacag ccagctgaac
     1261 tatatacatg ggatgaacca ttgaattatt tggatgtctt caatcaagaa caattagaac
     1321 aactgatctt gaacgtgaaa cctgccatgt tactagtgga acatgatcaa accttcctgg
     1381 ataaagtatc tactgagatt atttctcttg agagaatcta acccttgtaa tctcaagaac
     1441 aacaaggtat ggttaagggg aactttgcca acgaaggaga gaatcacatg ctttatgtaa
     1501 cgagacacgg agaaacaact tggaacgcac aaggattagt ttgcggacgg gcagatgtgc
     1561 cgctgactga aaaaggacag atgcaggcac agaaactagc agaaaaggta gtagatttgc
     1621 ctgttccaat cacgaaaatc atccattccc ctcttcaacg agcaagagat acagcacaag
     1681 cagttgctga tcgtctcagc ttgccgctga ccgttgatga acgattggta gaaatggatt
     1741 ttggggatta tgatggactg ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt
     1801 tcgctgtacg attcccaaat ggggagtcag tcttagatgt gtatgcacgg atcgttcctt
     1861 tattgaaaga atgcatcgaa gacgaagaaa atgtctatct gttagtttgc cataatgcct
     1921 tgataagagt gatcaatgct tattttcatc cgatgcctaa tgaggggttc tttacattca
     1981 tggtagataa tacagaattg gtttcttatg agtaattatg tttttgtgtt atttaaataa
     2041 aatatttcaa ggcactgctt ttgcttagca gtgccttttt taggttatga caattctttt
     2101 gataatataa actcaacctt tagaaatgaa atttaattat tttaataaaa gtattgaaat
     2161 tttgtttctt tagatatata ctctagtcat agaaatgaag acggatacag aaaaagaggg
     2221 ataatatgtt tggcttttca atatttatga acgattcttt gtcagaagag aagaaacagt
     2281 atatccgcca aatggcatcc aatggctttg tcggtatttt cacttccatg catataccag
     2341 aagatgatgt ttcggcatat caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga
     2401 ataaactaga attgatggtc gatatttcag gagaggcatt ggaacgagcg ggtttttcat
     2461 tagaagatct aacgcctttg aaagcaattg gtgtaacagg tttaagaatg gattatcata
     2521 tttccaatca gcagattgcc gactggtcac atcaattgaa aatcagtttg aatgctagca
     2581 cgatcacgcc taaagatatc gatgaattga aagaagcaga agctgatttc tcacagattg
     2641 aagcttggca taattattat ccacgtcccg aaacagggct ggacaaagaa tggtatcaga
     2701 aaaaaaatca atggctcaaa attcaaggtc tccttgtcca aggttttgta ccaggagata
     2761 cagagttgag aggcccacta tatcaaggat tgccaacttt ggaagaacat cgagggatcc
     2821 atcctcttgc aggcgctcta gatctattag caagtaacac ggatattgtt tacatcggag
     2881 atgcaggtct gtcagaaaac gttcaggagc aatttgcttc tttccaaaaa gaacagacag
     2941 tcttgctcca tacggaacct gtagatgaag aattttacga atatatatta gggaagcata
     3001 cgaatcggca agacgatgca agagatgtca tacgaagcgt agatgcacgt tttcgagaga
     3061 ttccgcctat cccagcaaga aatacagcta cgcgtatgaa agggtctatt actttagata
     3121 acgaaaagta tttgcgctat atgggtgaga tccaattaac caaatatgat ttgccagcgg
     3181 atgaaaaagt gaatgtagta gctaaagtta tcaaagaaga gctaccattg atcaaccaaa
     3241 tcaaagctgg tatgaactat caatttataa gaaaagaagg aagataaaaa tgagcaaaat
     3301 caatttagag aacctaacaa cggaacgcag aaaccaagaa acttttggat tagatgagat
     3361 gagtatttct caagccgttc aactaatgaa caaagaagat cagaaagtgg ccgctgcggt
     3421 tgaaaaagaa ctgacaagta tcgaaccagt gattgctaac acgattgagt cctttaaaaa
     3481 aggtggacgt ctgatttata tgggagccgg aacaagtgga agactaggtg tgttagatgc
     3541 tgctgaatgc gtaccgacat tcggtgtaga accggaaatg gtcataggtc taattgctgg
     3601 tggagaagaa gcaatgactg tagctgtcga aggggccgaa gattcttatg agctagggca
     3661 agaagatcta atcaagctga atttgacaaa aaatgatata gttattggta tcgcagctag
     3721 cggtcgtaca ccatatgtga tcggtggatt agattacgca aaatcgatcg gtgctgtcac
     3781 aggaacgatt tcttgcaata aacaagccga aatcagtaaa cacgcggatt tcccaatcga
     3841 agtcgatgca ggtccagaat ttttgacagg ctctactcga ttgaagtcag gaacagcaca
     3901 aaaacttatc ttgaatatga tttctactat cagtatgatt ggtataggta aagtattcaa
     3961 taacttgatg gtcgatgtca aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat
     4021 catcatgcaa gcgacagaag ctgattacga aacagctgaa aagtactttg aagaagcaga
     4081 tcaaaatgtt aagctggcta tcgtcatgat tttgacaaat agtgataaag aggaagctca
     4141 agcaaaatta gctgaagcaa acggatttgt aaaagggacc ttatgagggg gaaaagacca
     4201 tggcaaaaaa cgaactgact ttggaagaac tggcaaaaca aatttataaa ggggcaggcg
     4261 gcatgggcaa tgtagaaagc attgtccact gcatgactcg tgtgcgtatg aagatcatca
     4321 atgatgatct tgtaaatgtc tctgaattaa aagcgattcc aggagtgctt ggtgtagtag
     4381 aagatgaaca gcttcaagtc atcatcggac ctgggaaagt gaacaaagta gcacagcaaa
     4441 tggttgattt ggcaggcgtg aaattaggag aaaaactttc ttctgtaagt acagctacag
     4501 caaattcatc agcatctgga aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg
     4561 cacaaaaatc gaaacaaaaa ccatctaaaa tcaaatcggt attaaaagac atttctaata
     4621 tctttgtgcc aatgattcct gcatttgtcg gtactgggat cgtggcaggg attgccgcag
     4681 ttttagcaaa cttagtcaca gccggaacac ttgatgcagc aacatggcaa caatatatcg
     4741 atgtgatgaa catcttgaaa aacgggatgt ttagctacct agttatctat acaggtatca
     4801 atgctgcaca agtttttggt gcaacaccaa ctcttggtgg agttatcggg gccgttgtga
     4861 tgctgacagg gatgaatccg gaagcaccac tgaaaaactt atttactggt gaagctcttg
     4921 cagctggaca aggtgggatc ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg
     4981 agaaaaaact gcataaagta gtacctgatg cggtagatat catcgttaca ccaactcttt
     5041 cattgattgc catcggtctg attgaaattt tcttcatcat gcctttagca ggtttcattt
     5101 ctgatggcat ggtcggtggg atcaactggg tacttggcgt tggtggagca tttgccggct
     5161 tcgttctagg cacattattc ttgccgatgg ttatgttcgg actgcatcaa attttgactc
     5221 caatccatgt gcaaatgatt gatgaaactg gtcgtacgct attgcttcca attcttgcga
     5281 tggctggtgc aggacaagtg ggtgcagcat tggcattatg gattcgctgc aaaaaagata
     5341 aagaattaac agaaatgatc aaaggagctt taccagtcgg gatcctagga atcggcgaac
     5401 cattgattta cggagtaaca ttacctctag gacgcccatt tattactgct tgtatcggtg
     5461 gaggtatcgg cggggcagtg atcggaatga tcggaaatgt cggggctatc gctattggac
     5521 cttcaggagc cgctttgatc ccattgatct ctgacggtaa atggttaggc tacatccttg
     5581 gattattggc agcttatgct ggtggcttcg ttgctacatt cttcttcggt attcctaaag
     5641 accgtttaga aaaagaagaa ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa
     5701 aagtagtacc tcaaacttca gctgaaagtg tgactatcct ctcatcagtt gctgacggaa
     5761 cagttgtttc tttagataaa gcaagtgatc cagtttttgc acaaaagatg atgggtgaag
     5821 gctactttgt agaaccagaa aacggacata tctattctcc agttactggg gtagtcagct
     5881 ctgtcttccc aacgaaacac gctattggga tcacaacgcc aaatggttta gaaatcttgc
     5941 ttcatatggg aatcaacaca gttgacttag gcggtacgcc atttgatgtg aaagtaacag
     6001 aagggcagca agtgacacct gatacgcttg tagcagaagc tgacttagaa gcgatcaaag
     6061 cagcaggcaa agaaacatcc atgatcgtct tggtaaccaa tatggatcgt gtaaaaaact
     6121 ttgtcttgga aaaaacaggt aaagcaaaag caaaagcacc tgtgatggat gtcaaagctt
     6181 aatatgatag aaacaataga aacgaaagaa gaaactgaaa tctatttttc agcttcttct
     6241 tttttattat ttattgtttt ctgctaaaat ctgataaatt cgtgtcacat cagatggtgt
     6301 tccccgtaat tcataatctg ccaagaaagg aaagccaaat gtttgtgcat attgtttggc
     6361 tgttagacaa tactgattat tgaagttttt atttccgcta ccgactacac caagacaaag
     6421 tttatgattg tcttcatgtt ccagatattc acgcatcgtt tcggtcaaga tttctgtatc
     6481 accgttatct aaaccatttc cgccgtctaa ataagtagga acgaaagtaa aaaatggttc
     6541 tgtttctttc gtaaaatcac tattttcatg gatttctttt aaagtaactt caggattgac
     6601 aggatcttct gcatgcattt tttcagcata ttctgctaga tgtttggcaa aggcacgcgt
     6661 atttcctgaa atcgatatat ataaaatgtt catggatcat cctccaattc tttcctttta
     6721 gtatagcatc ttttttaagt tttttggtgg tgtaatttgg aaagggacta ggcgctttgc
     6781 ttgtataatg agaaaaaagc cggtagaatt atatttctat taaagttttt attaattaag
     6841 taaaaataaa aaaggtttgt tatacttttt gataaaagcg gttttatttt ggaggatgct
     6901 tacatggata cagagcaaca gaaaaaagga attggtttaa ttccacttgc tgccttagta
     6961 attggttcag cgattggagg tggcgtattt ggtattatga cggacatctc cggatcggct
     7021 ggcggcccag ctattttcag ttgggtactc gtaggaacag caatgttgat gctctccttg
     7081 tcgatcaata atatcaataa aaaacgtcca gatttaaaag gcgggatctt tagttacgca
     7141 gaagaaggtt ttggccgttt cgctggtttt atcagcggtt ggggttactg gctcacatgc
     7201 tggttaggca acgtagcttt tgcaacattg ttgatgagcg cattaggata tttcttccca
     7261 gtatttgaag gtgggcaaaa tctggcatcg atcgtgatga gtacagtctt tttatgggga
     7321 tatgcatggc tagtgaatcg gggagtcgaa aatgctagtt tcgtcaatgc tatcgtcaca
     7381 ttctgtaaat tagtgccatt atttttattt attgtagtag ctatcagcac gttttcgatc
     7441 caacaattca ttgataactt ttctagcgac attatattga atgcgaatgg acagccgatc
     7501 ccgttttcca gtcaagtatt gagttgcatc atggtgatgc tttgggtatt tgttggtatt
     7561 gaaggagcct ctgtcgtagg ttcacgagca aaaaaacaat cagatgttgg gaaagcaacg
     7621 attctaggga tattcggctt gattattatt tacgtcttgg tttccatgct tccatatggg
     7681 acaatgacga tgaatcagct gcaaacgatc aaggcgcagc ctgctatggg ctatgtgttt
     7741 gaaatgatgg ttggtaaatg gggagcagtc gtgatcaacg gcggtttgat catttcttta
     7801 gttggtgttt ggctctcatg gacgatctta ccgattgaaa caatgggaaa catggctgaa
     7861 gacggacttt tgcctaaagc atggggaaaa ctcaacaaaa atggggcacc aacttatgct
     7921 atcgtattga cgacgttatg tacgaatgtc tttttacttt cattattatt cactgacagt
     7981 gcttataact ttgcttacac acttggtaca gcagctattt tcttcacgtg gcttttctta
     8041 ggattgtacc aaatgaaact ttcttatcag cggaaagaat gggtccaatt tttagtaggg
     8101 cttttagcaa gtgcctttca agtatgggct atgctagtcg tagcctttaa tgaagtaatg
     8161 cttgttcttg ttcttttctt gccaggcatt tatttttacc tcaaagcacg taaggagcaa
     8221 aacgagcctg tacaacatat ccacttagat aagattttac ttataggtgt gacgatggtc
     8281 ggagtgattg caatggtttt atttgccact gggatgcttc acgtatagaa gtataaaaga
     8341 agtataaaga gaggttgtga gcctgtcgtt tattcaaaaa catactagga aaggagctgt
     8401 gacacaaatg ttgtcacagc tcctttttca gaataaacgg tgtttaagag acagccaatc
     8461 ggcaataagg ctgaacgtct ctttcacagc ctcttttttg atgagtcatc atacattttt
     8521 tcgcttaaat acgagatatc ctaatgctaa gaacaaaacg atgtaagcta aattaccgaa
     8581 tagcatttga ttagtagaaa gatgagtcaa ttgcttcatt atctcttcgt ttccaatttg
     8641 gttttgcaga ttcaacatac tgatcggatt ccatttgatc cactcccatt tttggatgag
     8701 tgcaataagg ataccagata tgattgacga agcaaaataa aagacgattc ctgctgagat
     8761 ggatgcgccg gaactattga taaagcaagc aatcatcaac actaaactta aaatcagcca
     8821 taaaccgata taactaccca aagtataagt gaacaagctt tggatcagtg tttgaccagt
     8881 agaaaccttc tccgtgaagg aaatcgaagg gaaaagaatc agtttcatca acacagtgac
     8941 aataatagct aaaaggtata aataaacaga ataaataact aatgtgatcc atttgcttac
     9001 taatatttct cctcgagaat actttcgata aaggagattt ttcaatgtac catattgtgc
     9061 ttccatcgaa atgatcgtgc ttgcagctgc aatcatgata aagacaatcc aagatgtggc
     9121 actaaaaagc tgagcagtca tttcaactgg atcaatgatc gtcgtgtatt ttttggctag
     9181 aagtgctgtt cctatgagca aaacaactaa tagaacagat aaaacagcag ttgacttttt
     9241 cttgatcaat ttaaataatt cttgttttac aagcaatgtc atttttcttc ttcccccttt
     9301 actgaatcca gaatttccaa tagagaagat tccaaatcat tcgtcagatg ctggacgtct
     9361 gtcacatgga tattttgtgc agacaatagc tggatcacgg ttcctaataa ttcttcgttt
     9421 tcttttaaaa gcttgatttt atcaggatct tccaaagtat atccagcttc tgtcaagatc
     9481 gttcgagcag ctagatcatt tgttgtagag ataacgatga aatcttgcgc tgtttggttc
     9541 aattcagcca ttgtacattg tttgatgatc tttccttgat tgatcaggat cagctcatcg
     9601 accatttttt caagctcgct gagtaagtga ctggagatca agaaagaggt gccctgagta
     9661 gccaagtcgg taatgatttt tcgcacatct cttgttgctt gtggatccaa accgttcatc
     9721 ggctcatcta aaataacgat agacggatgg ttcagtaagg caagagcaat tcccaatttt
     9781 tgtttcattc ccaacgagta ggatttcgct tttttgtgaa tgtacttttc catcttgagc
     9841 tgattcacga tagtgtcgat agcagataca tcgttggttt cagagaataa cttcagatga
     9901 tcatagcctg ttaagaatgg atagataccc gggtattcga tcaatgctcc tacttgttcc
     9961 aaccctttat gactagaggg agaaatctca ttttcaccaa tacagattgt tccatctgag
    10021 taatggatca gtcctaaaat agctttcatg atcgttgttt ttccagcgcc attaggacct
    10081 actaatccaa cgattttgcc aggggataag ctaaaactga catcgctgag ggcttgtttg
    10141 tgaccaaagt tcttggataa ttcttggacc gaaagacttt tcaaaaatat tcactccttt
    10201 tctatagata gtacaagcat acacctgatg agtggagaag tcgctaggtc attggactta
    10261 tttcttattt tttcataaaa aaattgaata ttaaataaaa aggcttattt tttctgatca
    10321 gtacttattc caagtgtcta gtggccgcta gtaatcttga tgccaatgat actgatgagt
    10381 agcatcccca caaagaacca agtggaaaga ttcagcttat catgaaacaa gaccacacca
    10441 attatgaccg aaccaactgc gccaatccct gtccagatag gataagagag actcatcggc
    10501 aaacttttga ttgcctttga caaaaagtaa aaactagcaa tcatgccaat cactgtgtaa
    10561 aaagaataat tgattttaga aaaaccatca ctccatttca tcatcgttga ccagaatact
    10621 tccaaaattc ctgctatgat taattccatc catgccatac ttacattcct ccttaactat
    10681 aaaaaaatac acaaaaagag gaaaatcatc ccttcaaaga cctaatggaa tgatcatcct
    10741 ctttatccta cccggtggca gattgtatat ctttttctgt agtatagcac acctatccag
    10801 atatcggcaa tctcatactt tttagtaaag tgtaccaagc actgacggat atatcgttag
    10861 cggaaaagaa gatgaatgct ttgcaatttc atagaaagaa gttgttattt attaatcatt
    10921 taaaaaaaac agtaactaga ccttatttct atatattatc gggcaataaa ttaaaaaaag
    10981 ctttacctta caataagtaa gataattttt gtgataaaaa aataacgaaa aatatttaaa
    11041 aatagtgtct atttcgtgag aaaacaacta tattttttat gtgttttgct ttataattaa
    11101 aaaaatattg tcattttatg gaggaaaata tatggaaaat tatcttagcc ttttaggcgt
    11161 cttgattgtt attctcgggt ttgcatttaa attagactcg atattgatcg taatggttgc
    11221 tttagttgtt acagctttga ctggcggtct tggcattcaa ggaatgttag aacttttagg
    11281 tacaagtttt gtaaacaatc gcgggatggc gatttttatc atcattatgt tggcaacagg
    11341 aacgcttgaa agaaatggtc tgaaagaatc agcggcttcc ttgatcaagc gattcaagaa
    11401 agtatctgct ggtatgattg tagatattta tgggatcttt cgaatgatct ttgcggcttt
    11461 taacgtgagt tttggtggag ttgctggatt tgttcgtcca atcattttac cgatggcatt
    11521 ggggaccatc gaatcaaaag ggttagaagt ccatcctaag catgaagaag aactaaaagg
    11581 tatggcttca gctatggaaa atgtctgctg gttctttggc caagtactat tcatcggagg
    11641 agcaggggcg ttgctagtcc aatcaacact gaaagatcta ggatatgacg taacattggg
    11701 acagcttgct ttagtggaag ttccagtggc tatcacggct ttagttgtgg caagtgtcta
    11761 cttctatctt aaagaaaaga aattagcgaa gaaatattat ccaggaaagg ggcaataaga
    11821 tggacttttt tacaaatcct gatattttat taggcgataa attactggag atcctttata
    11881 tcgtgatggg actcgtattg atttacactg gagtaagaaa tttaacagat aaaaccaatc
    11941 ctcatcgtta tgggtcagcc tttttctgga cggtcttagg ggtagttatt gcaggaggaa
    12001 gatggcttcc cgcaatcgtg aacggtggct tgatctttgc gatgaccata ccagcaattg
    12061 ctaagaaagt cagcaaagga gaaagccggc ttccatcaaa agaatatatg gaaaaaatgt
    12121 cagacaaact aggaatgaaa atttttattc cagctttgag tatcggtgtc tttgcgatct
    12181 tgtttgctct atttaccaat ttaggtgctt tagtcggcgt gggcgtcggt gtttttgcag
    12241 ccatgctaat cttgatgttc ttttctcgag ataatcgacc aagtacattt ttagatgatg
    12301 cagcagatat gctgggcaca gtagggccgt taagcatgtt gccaatgctt ttagcttctc
    12361 taggtgcggt atttacaagc gctggagttg ggacagtgat ttccaatgca gtaggtacga
    12421 tcgttccaga aggaaatgtg aacatcggca tcatcattta cgctcttggc atggtgatat
    12481 ttacggtaat catggggaat gcatttgcgg ccatcaccgt gatgacggtt gggatcggtg
    12541 caccattcgt atttagtcat ggagcgaatc cagcgttaat aggaatggtt gcattgactt
    12601 gcggtttttg tgggaccctg ctgacaccga tggctgctaa cttcaatatc gtgcctgtag
    12661 cgatgctgga gatgaaagac aaatacggag tgatcaaaaa ccagttgttt attgcgttat
    12721 ttatgctagt tttccaaatt gcttatatga ttctatttaa ataatagaag agaggggtta
    12781 tgacagaata attgtcgcaa cctcttctaa cattgtagtg gaacactatt tttttataag
    12841 aatatattaa gtttgatctg ttatgttatt taaactattg tttcctttat ttacgctgtt
    12901 caaggaaaat ggtatgataa aagataacca tatgttaaat caatcggacg gaggaacaac
    12961 agatgaaagt actagtaaca ggttttgatc catttggtgg ggacaaagtg aatccagcct
    13021 atgaagcagt aaaaaaaatg ccagatgaaa tcagcggtgc tgaaatcatc aaagtggaag
    13081 taccgaccgt attcgaaaaa agcagtcaag tagtcaaaga agccatccaa cagcaccaac
    13141 cagatgtcgt gatctgtgtc ggacaagcag gtgggcggtc agcagtctcg ttcgaacgtg
    13201 tcgcaatcaa tctagcagaa gcaagaatcc ctgataatga aggaaatcag ccattcgata
    13261 cggctttaga agaaaacggt ccagccgctt actttacctc attgccaatc aaagcaatga
    13321 caaaaaatgt acaagatcat ggtctaccag cgtacatctc ttatacagca ggaacatttg
    13381 tctgcaatga tatcatgtat cgtttgcttc atttgatcga aacacaattc ccggcaattc
    13441 gaggtgggtt catccacgtg ccattctcac ccgatcaagt gatcgatcgt ccagtgggta
    13501 cgccttctat gtctttagaa gacattgctt cttctttgac ttatgcagtt gaagcagcag
    13561 tagaaaataa agaagatatt tccggaaatg ccggaacaac tcactaattt agcaagaaac
    13621 actggctggt tgctaacgtt atgttagtag ccagcttttt gtaattagaa aggaaagaaa
    13681 cagccgtgga agaatggcaa tccgtctttg aagaatggtt tccaaaagaa atcagcaaaa
    13741 gctatcccat caaaatttct aaacaataca caagcagtca acgttgggag atctatgcaa
    13801 aattaacgaa aaaacagcgg gaactagtcg ataagcaccg ccgatatcta atcagctctc
    13861 gttttatgga agaacattat ttagctgcaa cagattgggt gttttctgac ttcaagatca
    13921 atccgttttt tcgaacaaaa cgaagccagc aaaaattgta ttgtgagtgt ggtagagaat
    13981 taaaagtgca atacatcgtt aaatcgccaa aaaccggaaa gatcctaaaa ctaggcatca
    14041 accactttgc cgatcacctg catgtctctc cgacagtggc agcttcgatc catcagggaa
    14101 tgacaaaagt tgatctggcc ttagacgaat tattatggct taagcagaag aatatcgatt
    14161 ttcctgaagg actatggcaa aaatactgtt ttgtcttgta ccaaaatcgt cggatgaaac
    14221 agccatattt accggatata aaattagctc agcggctcgc agaatttcgc caagtagaaa
    14281 tgccgattta catagcggat tatcaagcat tggaaaatga aatcaagaaa ataagtgaac
    14341 acatcaatgg gcagtcaaaa aagcgacaga taaaaaaaga actatttgac gactttgcag
    14401 aagaattagt aaaggatgta gaagaattct tgatcaacta tcgcgcattt ttacgtaaag
    14461 attggcaatc catcgtatat gaagaagtgc cagtacatcc taatgcttat tttgaaacat
    14521 ttatttccgt gttgagaaag acaaaacgtc aacgaacacc agaagtcact gctcagatgg
    14581 aatattttgc gaaaaaccaa cgattcatcc aaccaaaaat ctatctattt atttggaagc
    14641 aatattgtcg ctatggtttt acagaagggt tcttcgacag cattcctcgg atagtcagaa
    14701 atggtttttt aaaagtattg cgtaaagaac gagaagctat acaatctgct gataagaaag
    14761 accgtacagt ctcaaaagaa aaatggcaac tagtagtgaa agatatccaa tctggaaatg
    14821 tgcaagagac aatcgacaaa tggaaaggaa aacattatcg ttttactgaa gctcagaaac
    14881 aagcgttgga atattatcag aagttagaag aatcactgcg tttcaatgat gaggcaagaa
    14941 agtatctgaa ggaattacta taaaaaa
//
LOCUS       DACCKU010000074        14597 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.077, whole genome shotgun sequence.
ACCESSION   DACCKU010000074 DACCKU010000000
VERSION     DACCKU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.077"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            333..947
                     /gene="gmk"
                     /locus_tag="IVN13_002113"
     CDS             333..947
                     /gene="gmk"
                     /locus_tag="IVN13_002113"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP7012580.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            952..1257
                     /locus_tag="IVN13_002114"
     CDS             952..1257
                     /locus_tag="IVN13_002114"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7012581.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1510..3918
                     /gene="priA"
                     /locus_tag="IVN13_002115"
     CDS             1510..3918
                     /gene="priA"
                     /locus_tag="IVN13_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP7012582.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3933..4424
                     /gene="def"
                     /locus_tag="IVN13_002116"
     CDS             3933..4424
                     /gene="def"
                     /locus_tag="IVN13_002116"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP7012583.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4417..5355
                     /locus_tag="IVN13_002117"
     CDS             4417..5355
                     /locus_tag="IVN13_002117"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP7012584.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5355..6713
                     /gene="rsmB"
                     /locus_tag="IVN13_002118"
     CDS             5355..6713
                     /gene="rsmB"
                     /locus_tag="IVN13_002118"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP7012585.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6726..7466
                     /locus_tag="IVN13_002119"
     CDS             6726..7466
                     /locus_tag="IVN13_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP7012586.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7463..9532
                     /gene="pknB"
                     /locus_tag="IVN13_002120"
     CDS             7463..9532
                     /gene="pknB"
                     /locus_tag="IVN13_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP7012587.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            9697..10596
                     /gene="rsgA"
                     /locus_tag="IVN13_002121"
     CDS             9697..10596
                     /gene="rsgA"
                     /locus_tag="IVN13_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP7012588.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10609..11259
                     /locus_tag="IVN13_002122"
     CDS             10609..11259
                     /locus_tag="IVN13_002122"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP7012589.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11260..11898
                     /locus_tag="IVN13_002123"
     CDS             11260..11898
                     /locus_tag="IVN13_002123"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP7012590.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12053..12907
                     /gene="mreC"
                     /locus_tag="IVN13_002124"
     CDS             12053..12907
                     /gene="mreC"
                     /locus_tag="IVN13_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP7012591.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12911..13420
                     /gene="mreD"
                     /locus_tag="IVN13_002125"
     CDS             12911..13420
                     /gene="mreD"
                     /locus_tag="IVN13_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP7012592.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13675..>14597
                     /locus_tag="IVN13_002126"
     CDS             13675..>14597
                     /locus_tag="IVN13_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012593.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESS"
BASE COUNT         4898 a         2706 c         3177 g         3816 t
ORIGIN      
        1 gtattaagcc aaatttatgc aaaaatgtga gaaactattt tcacaaaatt tttcttcata
       61 ctcgaaacta atcggcttct tcacgacctc ttatacacgg cgttcaaaag cttgcatctg
      121 cgataataac cccaaccaaa cgaaaaacat gagaagctgt ttatgtttgg ctgttcttat
      181 tacttgatgc aggacagctt ttttacgacc tcttggtaca gggtagcttt tttaccaatt
      241 tctagctata attaaagata accttgaaag ttgcagaaaa aaagtgcaaa ataaacgata
      301 agtacaaaaa gactagttga aaggacgtcg tcatgtcaga gcgtggatta ttaatcgtgt
      361 tgtctggtcc atccggtgta ggaaaaggca cagtacgaaa agcaattttt gagagtgagg
      421 ataatgattt tcagtattcg atctcaatga ctacccgtaa aatgcgtgaa ggggaggtcg
      481 aaggcgttga ttatttcttc cgaagcaaag aagagtttga atcaatgatc gaagctggag
      541 agatgctgga atacgcagaa tatgtcggga attattatgg aactcctttg tcatatatca
      601 acaagacatt ggatgaagga aaagatgttt tcttggagat tgaagtccaa ggagcaaaac
      661 aagtcaaaga aaaagtgccc gatggtgtat tcatcttttt gactccgcca gatttagcgg
      721 aattaaaatc acggatcgta ggccgaggaa cagatgcaga tgaagtaatc gaagaacgaa
      781 tgaaagttgc aaaagaagaa atcgaaatga tggctcttta cgactatgct gttgtaaatg
      841 atgaagtgcc gctagcagtt gaacgaatca aaaacattat tgccagtgaa cattttcgag
      901 tagatcgcgt aatcggtaaa tatattaaaa tgttaaagga gatgtagtct tatgatgtta
      961 aaaccttcaa ttgactcact attagatcgt gtaaattcaa aatattctct agtgatttta
     1021 gcaagtaaac gcgcgcatga attggatgca agtgcacagc ctactctaga ttcttttgat
     1081 tcagtaaaaa gcgtcggtca agctttagaa gaaatcgatg caggaaatgt agtcaatgat
     1141 ccacatcctg aattaaaacg tgaacgtttg aagatggaag aagaggaacg tcaagcacaa
     1201 aaagagcgtg aacaacacga attggaatca cgtatccgtg aagagcaaaa aatgtaataa
     1261 agaaaaatag agcgaacatc taaaaagaaa agcaaaccca gctgtttcct tttcaaacat
     1321 tcgccactta ttgtctggta gaaaaggctg tgtctgaact ttttgacaca gttctttttt
     1381 ttcttatctg ctgttaattt tttgttagga tttggcagaa gataagacaa atccatcgat
     1441 ctctagtaaa ataggcaagg aactttttgt cgcttgataa gaaaagacaa aaaaaggagg
     1501 aaacgaacca tgacattaac agctgaagtc attgtggacg tacctactat gcagaccgat
     1561 cagccattca cttatttggt tccctctgaa atggaaacag ctttgcaagt cgggatgcga
     1621 gtagaagtcc cttttggtaa tggcaatcgt catgtccaag gatttgttat ggctatccaa
     1681 aaatcagaag aatcagctaa tccatctttg aaggcagtta ttcgtttgct agaccttgct
     1741 ccggtggtca atgaagaatt actttcatta gctgattata tgaaaaaaat tacttacgca
     1801 ttcaaaatca cttgtctaca aacgatgctt ccgagtgtga tgaaagcaga atatgataaa
     1861 ttgatctatc ctttggcaga cacaccagaa gttcaggtat tgtttgatca gcgagaagtg
     1921 atttcctgga aggaagcaga agaagaaggc agcctttctc aattgattcg ctggcgccaa
     1981 gagcaattag tagatatcaa atatgaagtc catacacgaa ataaagtaaa aacgattcgt
     2041 ttggtccgtt ctttgttaac tgaaaagcaa atcgaagaag aatgggcaaa attacggcaa
     2101 aatgccaaga aacaaaaaga acttcttttg tgtttatctg aactgagcca agaagaacca
     2161 attgcttatt tcaaaaataa aggaatcagt actgctgtct tgaatcaagg aaaagaaaag
     2221 ggctggctgg agtttataga atcagaacgt tatcgcgacc cttataaaga ccgagtattt
     2281 gaccaaacga cggcattgga actgaatgca gaacaaaaaa atgctgtcga acaaatcatc
     2341 acagcaggtc agcagcaaaa agatcaggtc tttttgctgg agggaatcac tggcagcggg
     2401 aaaacagaag tatatcttca ggcaatcgct gatgttttgt cagaaaataa aacggctatt
     2461 atgttagtgc ctgaaatcgc attgactccg caaatggtcg agcgattcaa aggacgcttc
     2521 ggagaatcag ttgcggtctt acatagcgga ctttcccaag gtgaaaaata tgatgaatgg
     2581 cgcaagattg agcgggaaga agcgcaagta gtagtaggcg cacgatctgc tatttttgcc
     2641 ccgctgaaaa atatcgggct gatcattatc gatgaagaac atgaatcaag ttacaaacaa
     2701 gatgagacac cgcgttatca tgcgagagat ctagcaattt ggcgcagtaa atatcatcat
     2761 tgcccaatag ttctaggaag cgcgacgccc tctttagaat caagagcacg agctcaaaaa
     2821 ggcgtttatc agttattgca gttaaaccac cgagccaaag cagctgcaca gcttccagct
     2881 attgagatcg tagatatgcg tgaagagttt cagaatcatc ggacctctac tttttctgct
     2941 aatttgcagg aaaaaataca aaatcgatta gacaaaaaag aacaaactgt cctactgctc
     3001 aatcgtcgag gatattcgtc ttttgtgatg tgtagggact gtggatttgt tcttccatgt
     3061 cctaattgcg atatctctct tacactgcat atggatacac gttcaatgcg ctgccattat
     3121 tgcggacatg aagaacctat tcccaatcgc tgtcctaatt gtggcggcaa taaaatccgc
     3181 tactatggaa caggtactca aaaagtagaa gaggaactcc gagaacttta tccgcaagct
     3241 aggattttgc gtatggatgt agataccact cgaagaaaag gggcacacga acagatttta
     3301 caaaaattcg gtgcaaaaga agcagatata ttattaggga cacaaatgat tgctaaaggt
     3361 ttggactttc cagaagtgac attagttggg gtgttgaatg ctgatacctc cctgaattta
     3421 ccggattttc gttctagtga gcatacattc caattgctta cccaagtttc aggacgagca
     3481 gggcgagcgg aaaaagcagg cgaagtcgtg atccaaacat tcaatcctca acattacgcc
     3541 atagaattag ctaaaaaaca aaattatgag cagttttatc agcaggaaat gcatgtacgg
     3601 catcgtggag gctatcctcc ttattacttt actgtgaaaa taaccgccag ccatcctgaa
     3661 gaacaagtag cggcaaagaa aatctttcag attgccaatc agctaaaaga agtgttaagc
     3721 cctcaaagct tgctattagg gccaacgccc agcatgattt tgagagtgaa aaaccgatat
     3781 tattatcaac taatcatcaa atataaacat gaaccgaatc ttcctcatgt tttggatgaa
     3841 attttgaatg gaagccagaa agaacagcga caaggtcttt ttgtggcaat tgacaacgaa
     3901 ccattaaact ttatttaaga aagggccaaa ttatgcgcta tcctatattg atccacccta
     3961 atgataaatt aaaaagaact gctcagccaa tcgatgtaat cacggacgag acaatcgctt
     4021 tattagacaa tttatatgag acaatgattg cgaatgacgg aatagggatc gctgctcctc
     4081 aagtaggaca aaacaagaga attgctgtga tcgaagtaga tgaaggcgag aaatttgaac
     4141 tgattaaccc agaaatcatc gaagcaaaag gtgaaagcct tgacgtagaa ggttgtctca
     4201 gtattccgca tgtctatgga acggtcaagc gagcagatga agtgaccgtg cgttattatg
     4261 atcgagacgg agaagagata gaagtgacgg cttttggcta tttagcccgt gccttccagc
     4321 atgaaatcga tcacttagac ggtatcttgt tcattgagaa aatgatacag cagatcccgg
     4381 aagaagaact tgaagaatat atggaggaac atcttgatga ctaagctagt atttatgggc
     4441 acacctgctt tttctgtgcc gattcttgaa gggctgatag aatcaggtta cgagatccag
     4501 gcagtggtaa cacagcccga ccgtccagtt gggcggaaac gagtaattac gccgacaccg
     4561 gtaaaagaag cggctctgaa gcacggtatc cgtgtgctgc agccagagaa aatctccggt
     4621 tcgccagaaa tggaagaaat cattgaattg gtgcctgatg tgatcgtcac tgcggcattt
     4681 ggccaattct tgcctgaaaa gttattgcaa gtaccaaaat taggggcaat caatgtccat
     4741 gcttctttac ttcctaaata tcggggaggt gcgcctgttc attatgccat catgaatggg
     4801 gagaaagaaa ctggtgtaac gatcatggaa atgatcaaaa aaatggatgc aggcggaatc
     4861 tatgcgcaag aaagcatacc aatcactaaa caagatgatg tagggacgat gtttgaaaag
     4921 cttagccttt taggaagaaa gctattgcta gaaacattgc ccaatatttt ggatggacaa
     4981 aaaccagtac ctcaagacga gtcagaagca actttttcgc caaacatcac acgggaacaa
     5041 gaagcaatcg attggcataa aactgcagaa gaaattgaca accaagtacg cggaatgcgt
     5101 ccttggccaa ttgcattcac gacctatgag ggcatacgct ggaaattatt atcggttgaa
     5161 gtattggcag aaactacaga caaagcacct ggtacgatca tccggaaaga caaaaaaaat
     5221 ctatggattg cttgcggtaa acagaccgtc ttagccatca atgaattaca gccagctggt
     5281 aaaggaaaac aggccgtaca tgatttcctt aacggaagcg gtcagcaagt tcagattggt
     5341 cagcaggtga aataatgaaa aaaatcccta aaaaaatgaa aaaatcagta cgatatatgg
     5401 cattactgac attagagcga gtgaacaagg gtggcgccta ctcgaatctt ttgttgaatg
     5461 agatgatcaa acaaagcgag ctaaatcaaa aagatgtggg tttatttaca gaattgatct
     5521 atggtacgat tagccatcag cgactattgg aattttacgt tgcaccattg atcgctaaag
     5581 caaaaaaagt ggatgactgg gtcaagacgc ttctttatct ttctgtatac caattagaat
     5641 ttttagataa agtccctgca catgctattt taaacgaggc ggtcgaaatc gcaaaagtaa
     5701 aaggaaatcc tgggacaggg aaatttgtaa atggtgtctt gaggaattat cagcgtcaag
     5761 gagcaccaga tctgcaaggc attgctgatc ctatcgaaag actttctgtc aaaatcagcc
     5821 tgcctctatg gatgacgaaa cgactggtgg atcaaatcgg atacgaagaa actgaaaagc
     5881 tcggtttatc gctatatcag cctagccatg ctagtgcccg agtagataca cgccgacttt
     5941 ccagagatca ggccatcact gttttgcaag aagaagagat cacagctgaa aaaagccaag
     6001 tcagtccata cggtatagta gcgaaaaaag ggcatttagc ttctagctca ttgttccacg
     6061 atggcgtcat gacgatccaa gatgaaagtt cgatgcttgt agcgccgtct atgcaaatcg
     6121 agcctgaaca tcatgtctta gatgcctgtg cagcacccgg aggtaagacg acgcatattg
     6181 cttctttttt agatccgtca tgcggtggag aagtggtcgc tttagatgta cacgaacaca
     6241 aagtcaaact cattgaagat aatgcagcac gtctaggtgt ctcttctgct gtctacgctc
     6301 aaaagatgga tgctagagaa gtcagtaaac agttcgaagc agagcagttt gatcggattt
     6361 tagtagacgc gccttgttca ggtcttgggc tgcttcggcg aaaaccagat attcgttata
     6421 aaaaacagcc ggatgaatta gcgaatttac caaaaatcca attggaaatt ttagaaagct
     6481 gcgcaccaac tttaaagtct tccggtattc tgacttatag cacttgtacg atcttaaaag
     6541 aagaaaacca agacgttgtt tctgctttct tggaaagaca ccctgatttt gagaggattg
     6601 atgttttgac caatcctatg ctacaatcat caatcgaaga gaaaatgttg actttgtatc
     6661 cgcatcagtt ttacacagat ggatttttca tttgttgttt gcgcaaaaag taatgaggtg
     6721 aaaaaatgca gattgaatat caatcagatg taggaagacg acgtaacaca aatcaagact
     6781 atgccagtgt atttaccagc cgttcaggtc tcaagcttgc tgtattagca gacgggatgg
     6841 gtggtcatcg tgccggagac attgccagtc agatggcggt aacgaatcta ggcgaagctt
     6901 gggaaaaaca agaagctgtt gacgatgaaa aaatcgccca atggttcatt caatcgattc
     6961 aagaagaaaa tatgctgatt tatcagcgtg gacaagaaca gccggaatat aacggtatgg
     7021 gaacaacaat cgtagcggcc gctctgtcag atgactggtt cacgattgcc aatgttgggg
     7081 atagcagagc ttatttgatt cgtgaagata aaatcatgca attgacggag gatcattctt
     7141 tagtcaatga actagtcaaa tcaggagaaa tcagcgaaga aatggcagtg aaccatccaa
     7201 ggaaaaatat tttaacacgt tctgtcggca tgcctggaac agtcgaagta gatgtttcta
     7261 ctcatctttg gcaactaaaa gatcgactgc tgttatgttc agatggcttg acgaacatgc
     7321 tgtctgaaga agaaatagga acgatcatca atcaagaagg cccattgtct cataaagtaa
     7381 ctgagctgat tgatcgtgcc aatgaagcag gcggagcgga taatatcacc gttcttctta
     7441 tcgaatataa ggaggacagt gtatgatcga gttgggcaaa aaattaaatg gccgttatca
     7501 aatcactgga aatatcggta gcggtggtat ggcaaatgta tttttggcgc acgatttaat
     7561 cttggatcgt gatgtagcag ttaaagtctt acgatttgac tttcaaaatg accagactgc
     7621 gattcgacga tttcagagag aagctttagc agcaacagaa ctcgttcatc caaacattgt
     7681 tagtgtttac gatgttggtg aagaagacaa catgcagtat ctggtgatgg aatacgtcaa
     7741 aggaatggac ttgaaacgat atatccaaac acattatcct gttccttatg aaacagcagt
     7801 aaatattatg cagcagatcc tttctgctat ttctttagct cataaccatc aaatcatcca
     7861 ccgagatttg aaaccccaaa acgtactgat cgataatgaa ggtgttgtaa aaatcacgga
     7921 tttcgggatt gctatcgctt tatctgaaac atcgatcacg cagacgaata cgatgctggg
     7981 atctgttcat tatctgtccc ctgagcaggc cagaggaagt atggcaacga agcagtcaga
     8041 tatttatgct ttagggatca tcctttatga gatgttgacg ggtagcgtac cgtttgatgg
     8101 tgaatctgct gtaacaattg ctttaaaaca ttttcaagat gatcttccat ctataaaagc
     8161 acttgatcca aatgtgccac aagcactgga aaacgtgatt ttacgggcaa cggctaaaga
     8221 acctgcagac cgttataagt cagccgagga aatgtctgat gatctttcta cagtattatc
     8281 accagctcgt gcaaacgaag aaaaatggca gcctcatgtg atggataatg aaacaaaagt
     8341 tatcacaccc cttacggaag atgcaccaat gcctgattca ttcaaatcta tgcctctgcc
     8401 aaaagacaag ccagaaaacg aaaccttgcc agaggaggaa gaagttctgc ctgatcctaa
     8461 aaagaaagga cagaaaaaat ggtggctgat cctattagct attcttgctg ttttaggaat
     8521 cggaacagct gtattctttg cttctggcgg gcgaggagaa gttgttgttc ctgatgtttc
     8581 tgatctttct gaagcttctg ctagagaaac actcgcaaaa gcgaaattag aagtagcaac
     8641 aaaaacagaa gaaattgcag atgacaaaat cgaagaaggt aatgtagtga agacagaccc
     8701 ggcagcggga acgacagtca agcaaaaacg cgaagtcact ctgtatatta gtagcggcac
     8761 gaaaaaaatc aaattagatg attacagtgg taaaagttat gaagatgccg aagcaagttt
     8821 gaaagaatta gggttttctg ctgacttgat tcaaaaagaa gaggaattca gttcagaggt
     8881 agaaagcgga atgatcattt cgcaatctcc atcagaagat acagaagtcg atcctaaaac
     8941 agacacgatt actttcacag tcagcaaagg accagaaaca gtgactttga gtagtcatgt
     9001 aggagaaaac tacgaaactg ttcgtaattg gcttgttaca caaggattca atatcaataa
     9061 tattagtgct acttatgact atagcgacac tgtaccatca ggttcgatca tcagtcagga
     9121 tccagcctcg ggtgaagttg tagcagaaga aacttacatc aattttgtcg taagccaagg
     9181 aacagagcct aaattgaatg atatctccgg ctatacaaaa agcgaagcac aaagttattt
     9241 agctagtgtc ggtgcagaat atatcgggca tgagacttcc gaatattcaa acacggtgga
     9301 taaagataaa gtgattcgta cagtaccagg ggcaggaacg acaattacca aaggaatggt
     9361 tgtgaacgta atttactcta aaggaccaga tccagcgtct tctagttctg aaaaagactc
     9421 ttcagacagt tctagcagct caagtagctc cagtagcaca agcaactctt caaaagaaga
     9481 atcttccagc tccacaagtt catcagctag cacctcagaa agttcaaaat aagtgctgaa
     9541 taaaatatat cagaagggaa tgcgacattg atttgttgca ttctcttttt tgtgtttcta
     9601 tctcttttga tcaattttca agccatatac tttgagaagt ttgcatggaa taacacctac
     9661 tttcctcttc gtatggtaaa ataaacaaaa acggaggtgg cacttatcaa aggacaaatc
     9721 agaaaagcaa tcagcggttt ttactatgta tacgcaaacg gagagaccta tcaaacaaga
     9781 ggaagaggaa atttccggaa tcggaaaatc acgccgcttg tcggagatca ggtgatcttt
     9841 gagagtgaaa atcaaacaga cggctatctt ctggaaatct tgccgagaaa aaatcaatta
     9901 gttcgcccac cagtcacaaa tgttgatctt ggaatcattg tcacaagtat cgtagagcct
     9961 gcattttctt ataatctact tgaccgcttt ctagttacct tggaatacga gtcgatcgaa
    10021 ccgattattt ttttgacaaa gaccgacttg tcagacgacc cagaaaaaat cgaagagatc
    10081 aagcaaacct atactaaaat aggctatccg gtgattgtac ccaatcatac gggtgatacg
    10141 acagaactga tccgttactt cccagaacgg ctatcggtct ttatgggtca gtctggagca
    10201 ggaaaatcga cgctactgaa tcaaatatca ccggaactga atttagcaac aggagagatt
    10261 tctgatgcac ttggaagagg acggcataca acacgtcatg tggaactgat tcctatctat
    10321 ggcgggttag tagcagatac accaggattt agctcgattg actttttgac aattgaagca
    10381 ggagaactgc cgagacaatt tcctgaattt gtagaagctt ctgtccattg caaatttaga
    10441 gagtgcatgc atgctaaaga acctggctgc gaagtaaaac atagagtaga aacaggtggt
    10501 atcgcacaga cacgttacga caattatttg caattcttac aagaaatcga aaaaagaaaa
    10561 ccaatgtatg caaaaaaaga aggtaaacga aaataggagg aaacaagaat gaagatagca
    10621 ccatccattt tgagtgcaga ttttgccaat ttacaaagag acattgagtt agtggaaaaa
    10681 ggaggcgcag attacatcca cgtagatgta atggatggcc aatttgtccc aaatatcaca
    10741 ttagggccaa atattgttca ggcaatccgt cctgttacta aattaccgtt agacgtccat
    10801 ctgatgatcg ttgatccaga aaactacatt ccagcatttg caaaagctgg agcagatatc
    10861 attactgtcc acgtcgaagc aactcctcat attcatcggg cacttcaaat gacaaaaagc
    10921 ttaggagtta aagctggagt agttatcaat ccaggaacac cagtctccat gatcaaacat
    10981 gttttgcctt tggctgatca agtactagtc atgactgtca atcctgggtt cggcggacaa
    11041 agctttattg aagaaacagt agaaaaaatt acggaactcg ctgatctacg tagagagaac
    11101 aattggactt atgcaatcga agtagatggc ggaatcgttc cagaaacagc aaaaatctgt
    11161 aaagatgcag gggcggatgt cttcgttgca ggatcatata tctatggagc cgaaaatcca
    11221 gtcggacaaa tcgaacgatt aaaagaggca ttagactaaa tgaaaatttt attagctgct
    11281 ggaggtaatc cgcaaaactg gccgcagtct ctgctgtcca aatacgatgc ctatgtcggg
    11341 atagaccgtg gtgcgctgta cctgcttcaa gcaggctacc cagtagatct ggcagtgggc
    11401 gattttgatt cattgtcgaa cgaagagcag aaggaagtat ttgcaaaagc caaagagtat
    11461 acccgttccc ctgcagaaaa agacgataca gatacacagt taggtatctt aaaaacattt
    11521 gaacgttacc cgcaagcgga agtcactttg attggagcta cgggcggaag attggatcat
    11581 cttttagcca atctctggtt aggtatggaa cctcgcttca aaccctttgc ccatcagctt
    11641 attatcagag attgtcaaaa ttatttgtct tattatttgc ctggagagca tatagtaaga
    11701 cagataccag atatgaagta tttagcttat tgctgcttga ctcccgtctc gaatcttaca
    11761 ttaaaaggca gcaagtatgt attggaaaat aaagaagtag aatatccgac atcttatgca
    11821 agtaacgaat ttctttcaga taccgcagaa ttttcttttt cagatggtat gattgcagtt
    11881 attcaaagca aagattgatc cataaacaaa taaaaaaatt tgtctttcag gctaaaaatg
    11941 acagaatcta ttatttgcta aaaaaaagct taagaggagc aacttacctt ttgtttcacg
    12001 gcatttagtg ataaaattga acacaagagt ctaattaatt cgaggtgtct ttgtgaagaa
    12061 gttcaatcct aataaaaata ttattattac cttggttatt gttatcatcg tggtgacgat
    12121 actaagtgtg accgctgcgc agcgagcgaa tgaaggaaag cccaatttct tccaatccat
    12181 cgttaatgac agtgttgcaa tggttgataa aacaatctct gccccagtac ggtgggtacg
    12241 aaatggagta gattctgtcc ataatctttt ttcaacttat gccgaaaatg agcggctaaa
    12301 agaaaaaatc gatagctatg atgacgtttc acagcaaaac aagaattacg agagagaaat
    12361 cgaagcgttg aaaaaagaac ttgagttgaa tcagacgctg actagttacg aaaaagtaac
    12421 ggcaaatgtc attactcgtt cgcctgacag ttggcaggac atgctgatcg tagacaaggg
    12481 ttctaaagac ggtatcgaag caaatatggc agtcatgtcg caaaaaggat tgattggccg
    12541 agtgatcgag gtcaatacgg cttcgtctaa aatcgaatta ctgtcatcct ctaatgaaag
    12601 ctccaatcat tttccagtac gggtatcttc ggctaatggc gaagcgtttg gtttgcttaa
    12661 aaactatgat gaaaagctcc atgccttagt ggtgacccaa ttaactggtg atacggatat
    12721 caaagaaggg gatgttgtcc agacatccgg tcttggaggg aattctccag ctaacttgcc
    12781 gatcggtacg gttattaaaa cgaaaccaga tagttatggg ctggatcggg aagtttatgt
    12841 gaaaccttat gcagaaatgt atgacgtgtc agttgtgacg attgtccaac ggctagcgga
    12901 ggattagtcc atgttgaaga aagaaacaat gaagtactat ctgccaatcg ttttgttctt
    12961 tttgatgttg atagatggtc atttaacaag aatgctaggg gagtggtcga aaggcaccta
    13021 tatgtcaaat gcccactttc tgatattggc attattatgt tgcagtatgg cgtttgaaaa
    13081 acgttattta ctgattacca cgattgttct cggggctatc tatgacgctt actatattgg
    13141 cgttatcggt atctatgcag tagctctccc tttaattgta tggttgatgt atgtaatgaa
    13201 agacgttatc catgtcaaca tctttactga atttttcagt atgatcatct ttgtcacggg
    13261 ttatgaattg tttacgatgg tggtccagtt gatttttaaa ttagcagtag taaataacac
    13321 gtattttatt acaaggtttt taggacctac actgctgttg aacatgatta tatttgtatt
    13381 attcattttt ccctttaaga aattattcag tgatgaataa tttctttttt tatttttagg
    13441 tcgaaagcga aaaagaatcg ttgttatctt aagaaatgct agacttaacg cggctttttc
    13501 atgatcattt tctgaaacga acagttttgt catttttaaa agttgttttg aaacattttt
    13561 gtaacataac tattatatcg ttgacacact gctatggtac aatttttata tcgttaagaa
    13621 agacttaaat atatttagat atttattaaa ttaagccagt cggaggaatg aagagtgaaa
    13681 aagagtttaa tatcagcagt aatggtatgt tcaatgacct tgactgccgt agcatcaccg
    13741 atcgctgccg cggcggacga ttttgattct cagatacaac agcaagatca aaagattgca
    13801 gacttgaaaa atcaacaagc agatgcacaa tcacaaatcg atgccttaga aagccaagtt
    13861 tctgaaatca acacacaagc acaagaccta ttagcaaaac aagatacatt gcgtcaagaa
    13921 tctgcacaat tagtaaaaga cattgcagat ttacaagaac gtattgaaaa acgtgaagat
    13981 acaatccaaa aacaagcacg cgaagcacaa gtaagcaaca caagctcaaa ctacatcgat
    14041 gctgtattga atgctgattc attagcagat gctattggac gcgttcaagc aatgacaacg
    14101 atggtaaaag caaacaacga cttgatggaa caacaaaaac aagataaaaa agctgtagaa
    14161 gacaaaaaag ctgaaaacga tgctaaacta aaagaattag ctgaaaacca agctgctttg
    14221 gaatctcaaa aaggcgatct tttatctaaa caagctgatt tgaacgtact gaaaacttca
    14281 ttagctgctg aacaagcaac tgctgaagac aagaaagctg acttgaaccg tcaaaaagca
    14341 gaagctgaag cagagcaagc tcgtattcgt gaacaacaac gtctagcaga acaagctcgt
    14401 caacaagctg cacaagaaaa agctgaaaaa gaagcacgcg aacaagcaga ggctgaagca
    14461 caagctactc aagcttcatc tactgcacaa agctcagcaa cagaagaatc atcagctaca
    14521 caatcttcaa tgactgaaga atcatcatca gctacacaat cttcagcaac agaagaatca
    14581 acaacacctg aatcttc
//
LOCUS       DACCKU010000075        14475 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.078, whole genome shotgun sequence.
ACCESSION   DACCKU010000075 DACCKU010000000
VERSION     DACCKU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.078"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..616)
                     /locus_tag="IVN13_002127"
     CDS             complement(<1..616)
                     /locus_tag="IVN13_002127"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP7012594.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGK"
     gene            complement(665..1300)
                     /locus_tag="IVN13_002128"
     CDS             complement(665..1300)
                     /locus_tag="IVN13_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7012595.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(1366..2769)
                     /locus_tag="IVN13_002129"
     CDS             complement(1366..2769)
                     /locus_tag="IVN13_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP7012596.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(2756..3787)
                     /locus_tag="IVN13_002130"
     CDS             complement(2756..3787)
                     /locus_tag="IVN13_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012597.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            3818..4039
                     /locus_tag="IVN13_002131"
     CDS             3818..4039
                     /locus_tag="IVN13_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP7012598.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(4204..4941)
                     /locus_tag="IVN13_002132"
     CDS             complement(4204..4941)
                     /locus_tag="IVN13_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP7012599.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(5094..6434)
                     /locus_tag="IVN13_002133"
     CDS             complement(5094..6434)
                     /locus_tag="IVN13_002133"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP7012600.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(6671..8194)
                     /locus_tag="IVN13_002134"
     CDS             complement(6671..8194)
                     /locus_tag="IVN13_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP7012601.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(8572..9183)
                     /locus_tag="IVN13_002135"
     CDS             complement(8572..9183)
                     /locus_tag="IVN13_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7012602.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(9306..9431)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(9513..10229)
                     /locus_tag="IVN13_002136"
     CDS             complement(9513..10229)
                     /locus_tag="IVN13_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP7012603.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(10235..10804)
                     /gene="scpB"
                     /locus_tag="IVN13_002137"
     CDS             complement(10235..10804)
                     /gene="scpB"
                     /locus_tag="IVN13_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP7012604.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(10788..11585)
                     /locus_tag="IVN13_002138"
     CDS             complement(10788..11585)
                     /locus_tag="IVN13_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP7012605.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(11555..11926)
                     /locus_tag="IVN13_002139"
     CDS             complement(11555..11926)
                     /locus_tag="IVN13_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7012606.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(11945..12832)
                     /gene="xerD"
                     /locus_tag="IVN13_002140"
     CDS             complement(11945..12832)
                     /gene="xerD"
                     /locus_tag="IVN13_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP7012607.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(12928..13401)
                     /locus_tag="IVN13_002141"
     CDS             complement(12928..13401)
                     /locus_tag="IVN13_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7012608.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(13506..14369)
                     /locus_tag="IVN13_002142"
     CDS             complement(13506..14369)
                     /locus_tag="IVN13_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7012609.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
BASE COUNT         4078 a         3092 c         2458 g         4847 t
ORIGIN      
        1 atttgccagt cgtatcgata cgaacagcat cctctgcttg cttcagcgga gagacgtccc
       61 ttgttgaatc ataatggtct ctttcctgta tctctttcgt cagtgtttca agatcagtcg
      121 gaattccttt ttcttggttc tctttgaaac gtctttctgc acgctcactt acgcttgcta
      181 ctaaaaagat tttcacttct gcattcggta atacagctgt cccgatgtct cttccgtcca
      241 tgacaacgcc accgcgttcc cctatttttc gttggacagc aactaattct tctctgactt
      301 ttccatgtgc agagacttca gaaacagctt ttgtgacatc tggttgacgg atttctaaag
      361 tcacatcttt tgatgctatg aatacatgct gtccatcttc ttgttgcgaa aaagagatag
      421 gatacttttt gatcagcgaa actagccctt cttcatctga aaaagataca ttgttcgtaa
      481 tagctaaata agttactgcg cgatacatgg caccggtatc tgtataaata taattcaatt
      541 cttttgctaa tatcttggca acggtacttt tcccagatga agcaggcccg tcgattgcaa
      601 tactgatttg tgtcatgata taactccttt ttctcagaaa aaaagccgca aagcgcggct
      661 attattattt aattcttaat tcttgaccag ggtaaagcat aaagttgttt ggatctaagc
      721 cattcaattc aaacaattga tcagtggtga tccctgcacg ctcagccact tgttttggac
      781 cttcgcctgc ttgaacagtc gttgttgaac cggctgcagc tgatgaagaa ggtgttgttt
      841 ctgctgcatt gctgcttgaa tcccctgctt gataagttgc ctcacttgag gaagtcggtg
      901 ttgtctctgc tgcaatactg ctttctgacg ttacacctgt atcttgagac acagtagatt
      961 cttccgcgat tgtactagat tctactgttg aagtctcagt tgactcctct tttgttgtac
     1021 ttgtagaact ttctactact gatgtcgaag tttcagaaga agttgctgct gttgcattgt
     1081 tgcttgatcc atttctgatc cagaaataag cacctgctgg aatagcgata cataagacca
     1141 gtaaaatcaa aaatagcgaa agaaataatg tatttccttt cttttgttgt ctctgagaac
     1201 ttcttgatga aacttcatca tctgtatcgt agattggttg ttcccatggt tcttgtgttt
     1261 cgttattttc tgtagattga tgtctatctt ttctgctcac tgttttacct cctaaactct
     1321 atcgtgtgta ttataccata ggaaaaagtg tcttgtccta aaaaatcaat gttccatgtt
     1381 ttttttaaat aaatttagta aaatcgtttc ccaatgaagt tgttcagtct tagaaaaaag
     1441 cttcctcttt ggcagaagtt ctgcttttgc tccatcaata tcacaacaat tctgcggctt
     1501 ttcggagagt ttttcgccaa aataagctaa gatgaatttt ctgcggcagt tttcttcatt
     1561 aatatagcga agcatctgct gcaggcggaa ttctttttct ttttcctgac gtttgagccg
     1621 ttccacccaa ttttctggtt tttcagactg ctggatcagc tccatccatt ttttttgcag
     1681 ctcatcaaag ggagcttgct ctgtggcata ttccaaataa agctcaaaac tttgtcgctg
     1741 ttctctagaa agttgattaa agaaataatg gatacgttca tccccacttt gataaaaaag
     1801 aattgctgca ctttcttcct gatctctgcc tgctcgaccg atttcttgta catagttttc
     1861 tagactgtct ggcaaatcat aatggaccac ataacggata tcagatttat cgatccccat
     1921 accaaatgca ttcgtagcaa tgagaaactg cagctgattt tttacaaact gctgttgaag
     1981 ttgtcttctt tgagctgcat caagaccacc gtgatagtat ccaactgtaa aacgttccct
     2041 gaataaatga tacaatcgtt cgacttcttt ttttgttgca caataaatga ttgccgctcc
     2101 ttgtgctctt tccatgaagt gttcaagttc ttgttctttt tgctgcgttt tccgaacaaa
     2161 aagcgagata ttttgccgat tgactgattg tctgatctct tttggtggtc gatcaaatag
     2221 tacatgacgt atatctcttg ctactaaatc tgttgctgtc gccgttagtg ccagtgtgac
     2281 cgggtttcct aaaagctttc ggatttttcc taactgttga tattctggac ggaaatcaac
     2341 tccccattga gacacgcaat gagcctcatc tactacatat aacgcaatat tttgtttttt
     2401 cagatgctct aaaagagaag gattagtcaa catttcggga ctcaaaaaca aaaatttata
     2461 ttgcgataga tgacggagga cgtatcttct ttcagattgc gaaagtaaac tattgaaagc
     2521 agctactcgt ttctcccctc gtttttgtaa ttgcgtcact tgatcttcca ttagagaaag
     2581 cagcggtgac acgatgatga ccagaccttc cattaaatac cccgtcaatt gatagcaaag
     2641 gctttttccc gttcctgtag gcaaaatagc gagagtatct tctccatcaa gcaacgcctg
     2701 aataatttct tcttggccgg gacgaaatga atcaaatcca aaatattttt ttaattcatt
     2761 tattaacatt ctgtatcgct tcctttctca atagatagat ttgatacaaa cgaaattctc
     2821 cataatctaa ttgtccatct acattccatt cgctaaagcg ccactctcgc acggaatcct
     2881 tatttggttc caaacgttcg attgtttctg cagagagaat ccactcgtat ggaaaatcat
     2941 caaaaaacaa ggcccactca ataagatgat ctgttactgt tcctcttttt aaatgacgga
     3001 ttgccatgat ctggtcaatt gtctgaccat tcataaacat ctgcttggtg atcatcatgc
     3061 tttgattgaa attttgctgt aataatgaat cgatcaaagc gtgccaaagc gaatctggcc
     3121 gtttttcgat ttgtgctaac aaaaggtgga tacatctgct ttgttgcaga taaaccgctg
     3181 actcatcatt gttttttgcc agctgataag gaagcagccc tgttcgctca tttccagata
     3241 attggttagc taaaaaatct gctgcttctt ctggtatctc cgaaaacagc tgcaccaaac
     3301 tctcatacgc agtatcaatc aaaatttcac gtggcaattg gctttcacta agccattttt
     3361 ttagttggaa agtataaaaa ggacttgtct ctgctggaag atagtcttga atacccgttg
     3421 ccagattaga aattacttga acagcaaatt taatgagccg ccaactagtt tcccctgttc
     3481 tgccatagcg gtcaaaacgc aaaccagtat gttctatttg atgttcggac aacatatcag
     3541 cccctgcagg cgttagcttt gcttcatttt catttatttc gatccaacct tgctgcgcaa
     3601 ttttttgcaa aatttgataa aacttatcct gcttcaattc tggaaaactg ttatggataa
     3661 acaaaagttc gtgggtaaat ccataaataa gtactgaagt cgttcgtttt cccacaagga
     3721 gttggtaaag agaactaatc cgcagcttgt gtcctgatga aaatagcgat aaaataaaaa
     3781 caatcatatt attctcctta agttaggatg gttagaaatg tcactactat gtaaacttgt
     3841 acctgaacgc tgtattgcat gcggtctttg tcaaatcgaa gcgccagata ttttcgatta
     3901 tgatgaagaa ggaatcgttt tatttgccca agaaccagag gctcatcaac agttcgtgat
     3961 gccggaagaa gaaacatatg taaaaaaagc gtatcaaaaa tgtccagttc gagcgatttt
     4021 attagaaaat aaaatataaa tataaacgaa actttaaaaa cgtacactaa gttctctaaa
     4081 cttgaaaata agccgggatc tgtttctctt gttccgtgaa atcccgactt attttgaaag
     4141 attcatttgc tatctatagt taattgacta gttttttcac ggaccctata ggacccattt
     4201 atattatttc aactttttca gccagttttc caatgtattg acgatcacag tttgctggtc
     4261 tgcgttagaa atttctgcct ctccatctcc tttttgctca ccatagctgc caaaacctcc
     4321 atgatttccg ccattgattg atacatactc tgttctttcc ggtaaatact gcttagcttc
     4381 ttgataagct tcctgattca atactttatc ttttgtcgcc gtgatcgata aaacaggaac
     4441 agctgattct gtcaatgttc cttttttgtc gggataactt gccatgaaga ataccccttt
     4501 taaatgtttg tctgaatgat tcttattgac ataacgactt gccattacgc cacctaatga
     4561 atggccaccg atcacataat cggaatttgg aaaatcttct ttgaccttat ccgctttgtt
     4621 tccgcccatc actgctagat caagtggcat tttgacaata gcgactgaaa agccgttttt
     4681 tgctaaatct tgcgcccaaa tactataact ttctggctct acgagagcac caggataaaa
     4741 aataagtaat ggagactttc catgattctt tttctctcct tcaaaaaata agtaatcttc
     4801 tttgtttttt ccttctttta ccaacacttg cgcttctgac gatggttcgt acgtattttg
     4861 ctgtatatag taataaccgg ctccaccgag taatcctaaa atgatgacta agccaattag
     4921 tattcgtcta aatattttca ctttctccca ccttttctat ctgagtgacc cctttttctt
     4981 aatcaagtat gattatacct taaaaaaata agagagaaat gcaaactttc cagcatcaaa
     5041 taaaaaaaga cggaatatcc atttgaatat ccgccctttt tgtatgttta tttttattta
     5101 gcacataaac gatacacagc ttctgcataa atttccattg ctttatacat actacttaat
     5161 tcccatcgtt cattcgcctg gtgcataaag tcaggagtat cagggaacat cgcaccgaat
     5221 gccacacact ggttcatcgt acgagcaaaa gtagcgccgc ctgatacaaa cggttcagtc
     5281 atgtctccag taatatcgcg atatgtcccc aatagatttt tcactaactc actgtctagt
     5341 ggcacgtata atgaatcaag gaagtcaaat tcttcataag tcaacccata tttttctgct
     5401 gttttagtta atttagctac taaatcatct ttcttgaatg taacagggat tctcatatct
     5461 acaccgattt ttgtttgttc tggtgtgatt tccaaacttg caaagttcat cgttagctcg
     5521 ccggattgtt catcttctgt tttgcccacg acagcttcac cagttgcatt ttcttgaact
     5581 aattttccta agaaattgat tgggccaaaa tcaaatactt ctgaaagagc aattgccaga
     5641 cgagaaatag cattcactcc ttgtgctgca tctttcgcgt ggatactttt accaagaacg
     5701 acgatgcctt caccttgttc ttcgaaatcg aattcgtgtt ttttcaatgc ttcttttact
     5761 tcagaaagct tttctccaga gtaaggagct gcatcaggaa caacattcaa cgctcctcca
     5821 gcttttacag aaaactcgtt cgttcctggt cctgtcaagt aagcttgcaa taagcctttt
     5881 tctgcataaa ttaaaggaaa ttcagcatct ggcgcaaatc cttgcgtaat tccttcttct
     5941 ttttcgttat atttttctaa acaacgccat aaattttctt catctgtacc aaagataaaa
     6001 cgaatacgtg tcttgaattc cacgccagca tcaatcaaag ctttgacggc atacattgcc
     6061 gcaatagaag gacctttgtc atcttgggat ccgcgaccaa ctaaccagcc gtcttttata
     6121 gttggatcga atggttttgt ttcccaatta ttttcgtccc ctgctggaac aacgtccata
     6181 tgacacaaga taccgaataa ttcatctcca gagccaatct ctgaataacc gtaatagcct
     6241 tctggatctt taaatgttct aaatcctagc ttttcactga tttccaatac tttatccaaa
     6301 gcttcaatca cttttttacc gaagggatgt ccttgtcctg aatctgcttc atctagtacg
     6361 gaaggaattc ggatcaattc gctcaatgat tctaatgctt gttcatgatg ttgttctgtc
     6421 acaaattttt tcatgatatt atcctcctaa tatgttaaaa agggtcatgt caaattcctc
     6481 cgacacaacc tcttcttctc aaataatgac gttttaagcc tgctccatca gcaagcagaa
     6541 tgttcttaac aaatgatgaa agaaaaaaca ctcactgaat ggagctattg acttatccac
     6601 gtgcttatcg atagccgttt atcggtttct tcattcttat gatagacctt gtacctgtat
     6661 ttgtctaggt ttagaacatt gctgctacac ctaggatcac acagatggct acaaataata
     6721 tcgcaattaa tttaccggta aatttccacc acactgaaac atcgaaacga gcaattgcta
     6781 cagcacccat tacgatagca gatgttggtg taattaagtt tactaaacca gatgcagatt
     6841 gataagcagt gattactaaa tccttaccga cgccggcaaa ttcacccata ggtcccatga
     6901 ttcccattgt tgcagcagct aagccggaag ttgaaggaat caagaatgac atagggatat
     6961 agaaaataaa tgtaataatg atgaatacac ttgaagataa tgaacttaat ccttgttcgc
     7021 cccaatgtaa gatggtgtct gtgattaatc cattgttcat aaccacttgg ataccacgag
     7081 caacagctac tacgattgct acaccgatca aatcagaagc accagctaaa aattcagaga
     7141 caaattcaga ctctttcatg ccataagtga ttgctactac gatggacatt actaagaata
     7201 acatagtaat ttctgggaag taccagtcac ctaatggaag catgctttta cctagaaccg
     7261 tacctagtac cggaaggttt gttaaccagt ttgtcaatga ttcaaaaatc gtgaaattcg
     7321 cattcaaggt tgtccaagga atcaaactga tgatcatgat accaaaagta ataaagaaca
     7381 atacattaac atgtttttgt tttttcgtga ttacttcttg acgaccgatt gcttcaatat
     7441 cgaaatgctt catatcttct ttacgttggt tgtatactaa tgattttgtt ggatcttttt
     7501 cgatctttga tgcataacga tacacatata caatagaaat agctaacaaa ataacgaaca
     7561 tcaagaatcg aagaccaata ccttcaccca tcgaaatccc aacagcttgt gaagctacgc
     7621 ctgttgcaaa aggatttacc gttgaagcta gacagcctac ttgagaccct actaatacga
     7681 ttgctacagc tactactgta tcaaagccta cagccatcat gactgggatc agtaaagcat
     7741 agaatggaat cgtttcttct gccattccgt aagtagagcc gcctaatgca aataacagca
     7801 tcaatactgg aataagcatt ttttcttttc ctttgaattt cttcacaact gaagcaatcc
     7861 cggcatctag tgcccctgtt ttgttgataa tacctaaaaa tccaccaaca accaaaatga
     7921 agaaagagat tgggattgca gcaggtgttt ctttagttag ctcccccgct ggtacaccta
     7981 gcatcccatt gattggcgcc atgaaaatat cccacagccc ttgaggattt tgttgtactc
     8041 gttcatatga tccagcgacg atgttgcctg catcgtctac actatagtgc ccagctggta
     8101 taaaccaagt aagtacagcg attactgcaa taataataaa taagaccgta tacgctgaag
     8161 gcatttgaaa ttttttcttt tttgtttgtt ccatcttctt cttcctttct tttccgttag
     8221 agacaacaat tttggctcta atgttatttt atcaaaaaga gtatcagaac aaaaataata
     8281 acacgcattt tgtaaaactt ttgttcaaaa agttcgtttt tccctattcc ctcctctttt
     8341 ttcaggtttt taaagctttc ttctttttca ttgtagcgtt tccttttttt tataaacaaa
     8401 cattttcgtc ttttaaaagg agaagaattt cacatttgca aagaattgat tgatttttat
     8461 gcataaatca aaaaaatact caaaacaaac aaataaaacg tttacattat tgcatgtctt
     8521 gtattttttt ccaaaaaaaa agaccaagaa atttcttgat ctttttgata tttatttgac
     8581 tgctgtatga cgttgttcga actgatgttg ttttcgactt aaccatggaa gaagttgcgc
     8641 atgcagcact aaaaagacag cactcacgat gaatcctttg atcaagttga aaggaacgat
     8701 cccaatcagt acatagtttg ctaaagacat gcccagcatc tgtgttgcgt tcagccctaa
     8761 aaacattaag taaagaggcg taataacgaa ataatttgcg atactcataa aaatagttag
     8821 agcgatggtt cctgtaatga cacctaaaaa tttgtttctt cctttatgct gtttctttac
     8881 actaaagaaa taataaatcg ggaatgtaaa gatcgttgtt gataagaaac tagcagtgtc
     8941 tcctaccaaa ttatccggag aaaaacctgt agtcagcaga tgtaatactg aacgcaagaa
     9001 cgctgttgca actccggccg ctggaccgaa taagaacatg ctgatcaata ccgagatatc
     9061 actaaaatcg atcttcaaaa aactaaaagc cggcatgatc ggaaaagcaa aatactgtaa
     9121 aactaatgcg atcgcagcaa acatcgctac tgcaaccatt ttctgtacac gggtgttctt
     9181 catttgaaat cctcctgtta ggactcctct tcagcgaact ttttgacgtt tctctccaaa
     9241 tgaaaaaact ctattttcca gggaaaatag agttagagtt atttgatcaa gcgtcgtcaa
     9301 ataagctcta tcttctttat ccagactata ctgtcggtat cggaatttca ccgattcagc
     9361 tatttttttg caaaataggt cgtggactat aaccaccggt cgggaatttc accctgcccc
     9421 tgaagacgaa ccttatataa tttttgttta catattcatt atacatatct attcaaaaaa
     9481 cgcaacatct aacacttata aaaagtaagt gttcattttg agtggttcag caaactgctg
     9541 acttcttttt tgttcagttc acggtaatcc ccaggacgta ggccttgtaa agtcagatct
     9601 ccgtattttt cacgttttag tttttgcaca ggaagaccaa ctgcttgaag catatttttc
     9661 acttgatgat tacgtccttc gtggatcgtc aattccacga tactgtggtt tgtcttagga
     9721 tccactgata aaatttggaa tttagctgga gatgtttttc gtccttcgat ctttattcct
     9781 tttgtcaaag gtgcaagcat cgattttgta gcgatccctt ttacctttgc aacgtaaact
     9841 ttatccactt catgttttgg atgtgtcagg cgctgagcaa aatctccatc atttgtcagt
     9901 agcaagatcc ccgaagtatc gtaatccagt cgacctacag gatagatacg ttcttttacc
     9961 atcggcaaaa gatccgtaac aacttttctt cctttgtcat ctgcaacagc agaaataact
    10021 cctttaggtt tgtacaataa atagtaaccg tattcttctt gataaattgg gacttcatcc
    10081 acttccacag tgtcatgttt cccaactttt atccctaatt cccttacaac ttgtccgttt
    10141 acttttacac gtcctgcaag gatgaattct tctgcttttc gacgagaagt gatgccagcg
    10201 tgggcaatta ctttctgcaa tctctccatt acaattattc tcctttattc attccatcaa
    10261 agaatagatc tggtgacacg tcttcttcaa gtgaaatttc catttcttgt gtatcaggca
    10321 gttcttctaa tgatttcaaa ccaaaataat ctaagaagta gccagttgtt ccatataata
    10381 tcggccgacc tggtccttct actctacctt tttcttcaat cagtttatgg gcagctagac
    10441 gctgtaccgc tcctgaaacc tgaactcctc gaatctgctc gatttcagct cttgtgattg
    10501 gctgtttata agcgatgatt gctaaggttt cgacggcggc ttgagataat gtgtttgatg
    10561 tcccttgcgc atatcgctta agtaagggag ccagctcttt tttagtcgtt aatacataat
    10621 ggctttccgt ttcaaaaata tgcaaagctg agtgcgagtt ttgagtgtaa gattctttta
    10681 attctttcac taaacttgtg acttctgctt cgctgagatt taacagtccg cttaattctt
    10741 cattactgat cccttcctcg ccaacaataa aaagaagggc ttcaagttca cttaatttcg
    10801 tcatgttcgg tatctcctac tgctggataa agcataattg attcatagtt ttctttttgt
    10861 tgcgcaatga tctgcccttt tttcatcaat tctagcaaag ccataaaagt agtgacaact
    10921 tcacttttag aaggtgactc aaaaaaagaa tcgaaagtac agccatgcgc atttcttttt
    10981 atacgctctt cgataaatac cattcgttct tcgatcgttg tgtcatcggg cgtgatcgtt
    11041 ttttccactt tttgagaaaa tttcttacgt tctaagattt tctgcatggc acggatcaaa
    11101 tcgatttttt tgacttgtcc tcgcttcaac ggcttttcag catccgctaa cccatcgatg
    11161 ttcataggtt cttttgtgta ataaagactt cgttcctctg ctttttctgt cagtctttca
    11221 gctgcatatt tatatttacg gtattctaaa agctgagtga ccaattcttc tcgaggatct
    11281 tcttcccata attcttcatc gtctgtgacg aattcttgga caggaagcaa tgtcttgctt
    11341 ttgattgcca tcaatgtagc agccatcact aagtattctc ctgcaacagc tagctcgagt
    11401 gttttcattg tatggatata attcatatat tgctccgtga tcgtagcgat cggtatatca
    11461 tatatatcga tttccatttg ctgaatcaga tgaagcagca gatcgagagg accctcgaat
    11521 acatctaatt taatattgat ctccgtcaac tcattcactc tttcttcttt ttctttgcta
    11581 gccattttcc aatgtaaggc tctgtgattt cagttgcacg gatggagaca tccgggtact
    11641 tttcctgtaa ttcatctaac atttgaaagc ccatattctc accgcgataa gacggattca
    11701 aagaaatgtc atggatgatc agctgatcgt tcgttgccca gtaaatacca ataatccctt
    11761 gaatgttggt tgaaccttct ggcgtaaaga aaaaaagttc ataatcgtct tcagattcgt
    11821 acgtatcaat ctccttcaac aaagaagttt tacctgtcag tttcttatgg aaactcaata
    11881 gtcccatagc aattttacgg ttttctttcc ggtaatgtgt taacatcttc tcaccttcct
    11941 atgactaagc ccttggaaaa tgctgcttat aaacatccgt cattcttttc ttcgtgatat
    12001 gtgtataaat ctgagtcgta gagatatccg cgtgacctaa caattcctgt actgtccgca
    12061 aatcagctcc attttctagc agatgtgtgg caaagctatg ccgcagcgta tgtggtgtta
    12121 cttctttgta aatcccagcc tctcgtacta actgcttcag atttttccag attccttgcc
    12181 gagaaagacc tttcccatga ttattgacaa atacatgcat ttcagatgcg tcctttacaa
    12241 gtaatggacg tgcttcgtct aagtagcgtt ccagccattg gatcgcataa tcacctaaag
    12301 gaatgatccg ctctttatct ccttttccaa ctgtctgtac cagtccaaga gaaagatgaa
    12361 gatctcccaa tttcaatcca atcaattcac tgacgcgcat cccagtagca tacatgactt
    12421 ccaagattgc acgatctctt atccctaacg tttttgtcgt atcaggtgct tcaatcagct
    12481 tttctacttc tgtcagagtc aatgtacttg gtaatttctg tgctttcttc ggcgtatcga
    12541 tatgttgcat cggatcatga tcagtgatac gctcttgccg taaaaactga tggaatcttc
    12601 tgagacttga gatcatccga gtaacagttg ctgtcgcttt tttttcttca tgcaacttct
    12661 ccagaaaacg gatgactaaa aagcgatcga catcctgcca tgaagcaatt ttctgctcct
    12721 gtagaaaatg caagtactgt tccaaatcac gttcatagct ttgtcgtgta ttggcggaca
    12781 atccccgttc aatattcaag tagtggagat aatcgttgat ttgctctttc ataataatcc
    12841 ccctcatgcc tctctatcat atcagatacc gcttcattct gatagtcttt ttccataatc
    12901 tgagcaaaga aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata
    12961 gcattcctga cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg
    13021 ttcttctact acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc
    13081 gcagttcaag catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata
    13141 acgggctaat ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc
    13201 aagggttcga taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa
    13261 gatttcttct gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc
    13321 tctttgcggt gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc
    13381 cgaaatagca ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa
    13441 aaagatataa cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg
    13501 gattctcaat tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct
    13561 tgtttcaaaa gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc
    13621 atgatttctt ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct
    13681 aaaatggtta aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat
    13741 gaaagattca gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct
    13801 agacgaggtt cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt
    13861 aaaacgtacg tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc
    13921 atttcttctg ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc
    13981 caaatacggt cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc
    14041 catagctctg tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga
    14101 ccgatatcga caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa
    14161 tgaccgatac ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg
    14221 taaccgaatc cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca
    14281 aaggtttgtc cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct
    14341 gtaaatatag ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct
    14401 taatacattt ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt
    14461 tggaaaggtg aaaaa
//
LOCUS       DACCKU010000076        14142 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.079, whole genome shotgun sequence.
ACCESSION   DACCKU010000076 DACCKU010000000
VERSION     DACCKU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.079"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1469
                     /locus_tag="IVN13_002143"
     CDS             <1..1469
                     /locus_tag="IVN13_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP7012610.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
     gene            complement(1599..4295)
                     /locus_tag="IVN13_002144"
     CDS             complement(1599..4295)
                     /locus_tag="IVN13_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7012611.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(4723..5796)
                     /locus_tag="IVN13_002145"
     CDS             complement(4723..5796)
                     /locus_tag="IVN13_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7012612.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(5793..6620)
                     /locus_tag="IVN13_002146"
     CDS             complement(5793..6620)
                     /locus_tag="IVN13_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012613.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(6621..7427)
                     /locus_tag="IVN13_002147"
     CDS             complement(6621..7427)
                     /locus_tag="IVN13_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012614.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(7439..8524)
                     /locus_tag="IVN13_002148"
     CDS             complement(7439..8524)
                     /locus_tag="IVN13_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012615.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(8541..9083)
                     /locus_tag="IVN13_002149"
     CDS             complement(8541..9083)
                     /locus_tag="IVN13_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7012616.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            9341..10330
                     /locus_tag="IVN13_002150"
     CDS             9341..10330
                     /locus_tag="IVN13_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP7012617.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10327..11064
                     /locus_tag="IVN13_002151"
     CDS             10327..11064
                     /locus_tag="IVN13_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP7012618.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            11061..11612
                     /gene="coaC"
                     /locus_tag="IVN13_002152"
     CDS             11061..11612
                     /gene="coaC"
                     /locus_tag="IVN13_002152"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP7012619.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            11628..12260
                     /locus_tag="IVN13_002153"
     CDS             11628..12260
                     /locus_tag="IVN13_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP7012620.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            12345..12992
                     /locus_tag="IVN13_002154"
     CDS             12345..12992
                     /locus_tag="IVN13_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7012621.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            12997..13980
                     /locus_tag="IVN13_002155"
     CDS             12997..13980
                     /locus_tag="IVN13_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP7012622.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         4404 a         2858 c         2572 g         4308 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgtac aggtactgtt ctatggggcg cttacttaaa aaaagtgtct
     1561 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
     1621 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
     1681 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
     1741 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
     1801 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
     1861 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
     1921 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
     1981 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
     2041 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
     2101 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
     2161 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
     2221 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
     2281 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
     2341 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
     2401 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
     2461 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
     2521 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
     2581 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
     2641 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
     2701 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
     2761 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
     2821 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
     2881 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
     2941 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
     3001 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
     3061 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
     3121 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
     3181 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
     3241 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
     3301 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
     3361 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
     3421 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
     3481 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
     3541 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
     3601 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
     3661 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
     3721 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
     3781 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
     3841 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
     3901 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
     3961 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
     4021 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
     4081 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
     4141 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
     4201 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
     4261 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
     4321 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
     4381 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
     4441 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
     4501 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
     4561 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
     4621 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
     4681 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
     4741 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
     4801 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
     4861 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
     4921 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
     4981 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
     5041 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
     5101 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
     5161 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
     5221 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
     5281 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
     5341 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
     5401 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
     5461 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
     5521 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
     5581 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
     5641 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
     5701 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
     5761 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
     5821 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
     5881 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
     5941 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
     6001 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
     6061 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
     6121 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
     6181 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
     6241 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
     6301 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
     6361 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
     6421 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
     6481 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
     6541 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
     6601 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
     6661 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
     6721 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
     6781 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
     6841 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
     6901 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
     6961 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
     7021 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
     7081 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
     7141 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
     7201 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
     7261 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
     7321 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
     7381 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
     7441 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
     7501 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
     7561 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
     7621 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
     7681 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
     7741 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
     7801 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
     7861 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
     7921 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
     7981 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
     8041 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
     8101 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
     8161 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
     8221 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
     8281 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
     8341 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
     8401 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
     8461 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
     8521 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
     8581 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
     8641 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
     8701 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
     8761 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
     8821 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
     8881 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
     8941 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
     9001 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
     9061 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
     9121 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
     9181 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
     9241 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
     9301 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
     9361 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
     9421 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
     9481 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
     9541 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
     9601 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
     9661 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
     9721 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
     9781 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
     9841 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
     9901 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
     9961 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    10021 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    10081 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    10141 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    10201 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    10261 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    10321 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    10381 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    10441 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    10501 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    10561 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    10621 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    10681 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    10741 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    10801 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    10861 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    10921 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    10981 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    11041 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    11101 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    11161 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    11221 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    11281 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    11341 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    11401 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    11461 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    11521 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    11581 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    11641 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    11701 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    11761 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    11821 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    11881 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    11941 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    12001 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    12061 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    12121 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    12181 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    12241 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    12301 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    12361 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    12421 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    12481 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    12541 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    12601 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    12661 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    12721 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    12781 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    12841 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    12901 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    12961 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    13021 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    13081 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    13141 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    13201 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    13261 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    13321 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    13381 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    13441 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    13501 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    13561 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    13621 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    13681 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    13741 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    13801 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    13861 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    13921 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    13981 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    14041 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    14101 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aa
//
LOCUS       DACCKU010000077        14094 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.080, whole genome shotgun sequence.
ACCESSION   DACCKU010000077 DACCKU010000000
VERSION     DACCKU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14094
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.080"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(384..1670)
                     /locus_tag="IVN13_002156"
     CDS             complement(384..1670)
                     /locus_tag="IVN13_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7012623.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(1663..2931)
                     /locus_tag="IVN13_002157"
     CDS             complement(1663..2931)
                     /locus_tag="IVN13_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP7012624.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            3183..3644
                     /gene="mscL"
                     /locus_tag="IVN13_002158"
     CDS             3183..3644
                     /gene="mscL"
                     /locus_tag="IVN13_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP7012625.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(3703..4149)
                     /locus_tag="IVN13_002159"
     CDS             complement(3703..4149)
                     /locus_tag="IVN13_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012626.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(4242..4487)
                     /locus_tag="IVN13_002160"
     CDS             complement(4242..4487)
                     /locus_tag="IVN13_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012627.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(4602..5180)
                     /locus_tag="IVN13_002161"
     CDS             complement(4602..5180)
                     /locus_tag="IVN13_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7012628.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(5257..5787)
                     /locus_tag="IVN13_002162"
     CDS             complement(5257..5787)
                     /locus_tag="IVN13_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP7012629.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(5791..6651)
                     /gene="rlmB"
                     /locus_tag="IVN13_002163"
     CDS             complement(5791..6651)
                     /gene="rlmB"
                     /locus_tag="IVN13_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP7012630.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(6644..7039)
                     /locus_tag="IVN13_002164"
     CDS             complement(6644..7039)
                     /locus_tag="IVN13_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP7012631.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(7036..8448)
                     /locus_tag="IVN13_002165"
     CDS             complement(7036..8448)
                     /locus_tag="IVN13_002165"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP7012632.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(8445..8981)
                     /gene="cysE"
                     /locus_tag="IVN13_002166"
     CDS             complement(8445..8981)
                     /gene="cysE"
                     /locus_tag="IVN13_002166"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP7012633.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(9303..10763)
                     /locus_tag="IVN13_002167"
     CDS             complement(9303..10763)
                     /locus_tag="IVN13_002167"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP7012634.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(10933..12090)
                     /locus_tag="IVN13_002168"
     CDS             complement(10933..12090)
                     /locus_tag="IVN13_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP7012635.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(12124..13494)
                     /gene="radA"
                     /locus_tag="IVN13_002169"
     CDS             complement(12124..13494)
                     /gene="radA"
                     /locus_tag="IVN13_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP7012636.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(13557..14084)
                     /locus_tag="IVN13_002170"
     CDS             complement(13557..14084)
                     /locus_tag="IVN13_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP7012637.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         3703 a         3076 c         2524 g         4791 t
ORIGIN      
        1 ccaaccaagt taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc
       61 agggcagctt tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat
      121 aagccgaaat atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga
      181 agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag
      241 ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag
      301 aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag
      361 acttttcctc ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct
      421 tcacttataa atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt
      481 tcaggaagat caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata
      541 tattcgatcg aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg
      601 agatttgttt ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt
      661 tctgctgctt tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga
      721 tccaagctga attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga
      781 tagtttcgag aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag
      841 cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat
      901 ttatctcgtg taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca
      961 gggaaataac gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga
     1021 ataagtttca tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt
     1081 ggctgataaa acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt
     1141 tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg
     1201 ccaaaaaaca tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt
     1261 cctttttcct tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga
     1321 gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa
     1381 cttgtgaaag cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct
     1441 tctttttcaa acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt
     1501 tctttttcac catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa
     1561 ccatatgtct tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt
     1621 acttcatgat aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc
     1681 tcctgctaaa aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt
     1741 gatcgttacg ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag
     1801 ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata
     1861 ttggtttctc agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg
     1921 caaactttct aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat
     1981 accggtttgg acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc
     2041 tagactttct ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa
     2101 accgttaaag accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata
     2161 agctaggttc agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt
     2221 cacttgaggt tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa
     2281 ttgcccaact gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat
     2341 ttgatcattt gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc
     2401 caaatcttcg actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag
     2461 ttcttgcaag gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag
     2521 acgcagattg ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt
     2581 tggataaaat aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt
     2641 gacatattca ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt
     2701 tctaactgat aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt
     2761 ctgatccgga taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt
     2821 gatgacttct tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg
     2881 ttttgttggt aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg
     2941 ctccttttca cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact
     3001 tattcctctg cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta
     3061 cactttatag tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata
     3121 ttgttttttt attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat
     3181 tcatgatcaa agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg
     3241 gcgttgttat tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca
     3301 caccactaat aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac
     3361 tttcaattct tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta
     3421 gtgctatcat tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa
     3481 accgtgcgaa gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg
     3541 cagaagatta tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata
     3601 cacatccagt agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa
     3661 aagaaagccg attttcagtg tcggctttct ttttttagat tcttaacttg ctacttgttc
     3721 ctttttcatg tcgatatgtg gaatatcatc ttccaaatac acatctgaga ttgccttgaa
     3781 tccaaacgac tcataaaaag cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc
     3841 tggaaaattt ttctggatca tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc
     3901 tttacggttg tcctgatgaa caaggacacg tccaaaatgg atactctccg cttcttcata
     3961 tactcgcgtg taggcaagga tcttcccatc agtatcttga aaaaacgtat gccaagcttg
     4021 tccatcaatg tcatcaactt cttgataagg acactgttgt tccacaacaa aaactgcgat
     4081 tcgttctttg acgatcgcgt aaaactctgc tgttgacaat tcttcaaatt tctttacctg
     4141 atattccact aagcaaacgc cccctgcttt tttattctta gcataccgat tcgcacacaa
     4201 aaaagcaaac gaaccttacg aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa
     4261 aattcgatct caactgtccg agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa
     4321 gaattttctt caggatccaa atcaacaacg aatactgaag gataggtttc tgttaaaacg
     4381 ccgcggcgtt ctgtttgacg ctttcttcct gtctgagcaa ccaacatgat tgggctgccg
     4441 atacgacctt ctaaatcttt cttgattgaa gctaaagttg ttggcataca gttcacctct
     4501 actatcaact ataccacata tttttaaaat tttcaagttt accataaaaa aaaaagaaat
     4561 gcaataaaaa taaataagaa gctcattaag atttttctaa attattcctc attataaaat
     4621 ttcaagtgtt tttttatttt gagttttact ctcccgtaag cgctcattac ttgcttgtga
     4681 tccattgcta aacattcagc catgtctaca aaatcaacgc cttgcatata ataatgaaaa
     4741 attttagctt ctatttttga aaactggatt tcgcaatttt cgattgcttc ttttaataaa
     4801 agatgctgaa tcgctaaagg ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct
     4861 gtctctattt tttggtcgat tgagacttca tctttttgat cacgacgctt cagcgcgttc
     4921 tgcttcctca acagagaata gatttgattg cttataatgc ttttataata acttccaaag
     4981 ctaacacctt tgcttttttc gtaagttctc aatgcctgat ataaagcgat ccttgcttct
     5041 tgttcccaat cctcagaaac ataatttctt aaataatagg tgtttttcaa acgtagtaaa
     5101 agtggaaaat atttttgata cagggcagca aatgcttctt cgtcaccatt acgtgcagcg
     5161 cgcaataact cctcagacat aaaataattc ctccataaat tcgtttatgg aggaatctta
     5221 tcatcaatga ttcatcgaac tgattagaac gatatgttat ttattttttc tcattaaatc
     5281 gtccaatttt tgggacaatt tttttaactg ctcttcattc catggagaat tacgtcggaa
     5341 atcaataaaa tgcatatctt tattttcttg ggctattgtt ttttccgttt tttttatcgt
     5401 cttatataat tcataagcag aagttcgtaa tgctccttgg gaaaagatca tccactgttc
     5461 tgctaaatcg cttgtcgcca ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat
     5521 tctctcgata taactgtcag ccgtttcgtc ttctttcgta aaaacaacat ccaactggta
     5581 tttcttataa ttttgttgga tgcccggaac tagttgggca tcgaagacaa cgatgacttc
     5641 cattccttca tatttcgcat aatttgataa acgatgtaaa agagcttctc ttgcatccgc
     5701 cattttgtcc tgctttttaa gctggacaag ttccggccac gaaccaatca tgttgtagcc
     5761 gtctacaatc aacagctgtt ttttcatggt ctactccccc tgacgttggg aaaaagcctg
     5821 atacataagc aaaccagcag caacactggc atttaagctc tggacgtgcc cggtcatcgg
     5881 gatcgtcagc aattcatcca tttctttttt caatcctgga ctcattcctt tgccttcatt
     5941 accaatgact aaagcaattg atcctttggc attccatttt tggaagtttg tgccggtcat
     6001 atctgtgcca aatacccaaa atcctgtttc tttgagctgt tgcacagctt gtaccaaatt
     6061 cgtcacgcgt gcaacaggga catgttcgac tgccccagtg gaagtctttg ttacgatagg
     6121 tgtgattcca actgctcggt gttttggtat gatcacacca tcgacgccac ttgcgtctgc
     6181 tgtccgtaaa atcgacccaa aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa
     6241 ggggtcttct tttttctctt ttgttcgttc aatcagttgg tcaagggata aatattcata
     6301 aggtgtaatc gcaagaacga ctccttgatg cacgccgtga tcgctcaaag tttctaattt
     6361 ttgtttagga acccatttga ctggcacagc ttgttcacgt gcgatttctt tcagttcttc
     6421 tactttttct cctcgtgaat cttcttgaag aaagagttta ttccctcgcc cttgttgtag
     6481 cgcttctact gcggcatggt gcccaaagac gaaattatct tctaatgctt cttcattttg
     6541 atcagaaact gactgatttc gtccagtttg ctttttattt gaatatctat tttgtttttt
     6601 gtcttcaaat ccacgtttcg attgatgttt gtttgttctt tctttaccca tttttttcac
     6661 caactttctg aatacaccag cggatcaatt cttccatccg ctcggtttgt tttgtcaaat
     6721 gcagatagcc catgagcgct tcgaaccctg ttgcgacccg atacgtcgta acatcagcat
     6781 tttttgcgct cgtatggctt ttagcatttc ttcctctgcg ataataaagt tcttcttctt
     6841 ctgtcaaaag atgttcttcc agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa
     6901 cataattcgt tgccgcacga tgcagcttat tgggttttgt ttctccttga tcaatcaggt
     6961 ggtcacgaac atagatttcg taaatcgcat ctccaacata agccaaggct aaaccgttca
     7021 attgtgtgta atctctcatg cttctcttct ccatcgggtt ccttgcggtg tatcttctaa
     7081 taagatccct ttttctttca acaaatcacg aatttcgtcg cttcggacaa aatcacgatt
     7141 gcttcttgct tgatttcgtt cttcgatcaa ttgttcaatc tcttcatcta ataactcttc
     7201 tgctgtaaat aaaataccga agatcgaaag ccattgcgag aataaagctt ctccttgtgc
     7261 caagacagcg gcagaaactt tcggctggtc tagataagtg ttcagccact tagcaagctc
     7321 atagaccact gtgatcccat ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg
     7381 ctgttctaaa ttttctaaca aagcgatttt atccgcatca tctgccaaat tctctgaggc
     7441 agtttcttgg cggaatttca aattgtcaaa cgtattcttc agtcgttgta aattggtggc
     7501 tgcttctttc atcgtcgttt cactgtagcg gatcggacga cggtattgtg tggtcgccat
     7561 gaagaaacgc aagacttgtg gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt
     7621 ccctagagat ttactcattt tttcatcgtc ttcgccaatc gttacatagc cattgtgcat
     7681 ccagtaatta gcgaattttt tccctgtttt cgcttcactt tgtgcgattt cattttcatg
     7741 atgaggaaat tctaaatctt gcccgccacc atggatatcg atcgtttctc ctaaatgttt
     7801 tgttgccata acggaacatt caatatgcca accgggacgt cctttgcccc atggtgaatc
     7861 ccaagaaatc tcgccaggtt ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt
     7921 ttgctgctgt tcttcccctg tacgctgact tgctccgact tccagttcat cgatggattg
     7981 gtcacttaat ttcccataat tttcaaattt acgggtacgg taatacacat cacctgaaga
     8041 ttcataagca tagccttttt caaccaacac ttgaataaat gctaaaatat ctggcatatg
     8101 atccatgact cttggatgca gtgtagcagg ctcgacattc aacgctttcg tatcttcttc
     8161 aaatgctcgg atgaaacgat cagcaacttc tggtgctgta atacctaatt cgtttgctgt
     8221 acggatgatt ttatcgtcta catccgtgaa attcgatacg aaatcgactt tatagcctcg
     8281 atattcaaaa tagcgtcgaa ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg
     8341 gatataatta tacacagtcg gtccgcagac atacataccg acatgtccct tttgtatagg
     8401 gatgaattct tctttttttc tggtcaatgt attgtagatt tttatcattc tgatctcctc
     8461 actttttctc ctttgattcg aacgactttt gctggtactc ctaccgctgt agcgtcgtct
     8521 ggaacatctt tcaacacaac ggcacctgca ccgattttgg cgttttttcc gatcgttacc
     8581 ggtcctaata tctgcgcatt cgcagagagc atggctcctt gtttaacagt tgggtgtctt
     8641 ttacccgttt cttttcctgt gccgcctaat gttacgccat ggaataaaat cacatcgtcc
     8701 tcgatttctg cggtttcgcc aatgacgatt cccattccat ggtcgatgaa cacgccagtt
     8761 ccaatcgttg ctcctggatg gatctcaaca ccagttagga aacgccagaa ttgagcattg
     8821 atcttcgcca ataaaaagag ccggtgctga tataaaaaat gagagaaacg atgccaaaac
     8881 aaggcatgaa aaccagggta tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg
     8941 tttctctttg ctgcatcaat cgctcgggta aaccaactca tgactttcca ctcctttcgt
     9001 aagaatttta tgtaggaaat catcttctta taaagaaaag aagcgccttt taaataagtt
     9061 tccttatcaa aagacgcttt ccgcgtggtt ccacttttct tcataatcaa tgattatctc
     9121 tgacagctaa cgcctgttat acgttcttgg ctactttgtt tcctataaaa cagtttttcc
     9181 tgttttctgt gtttcgccaa gaccactccc agaggcattt cggcaaccat tcattgatac
     9241 cttgcaccac ccggtatctc tcttgtaatg atcagctgct tacttttttc tgttcatcgt
     9301 gtttagttca gtacttgatt taaatgtgct aatgattttt cacgaccaag caattcgatc
     9361 gtttcaggca attctggacc atgcatttgt cctgatacag ctacacggat tggcatgaaa
     9421 agattttttc cttttacgcc agtttctttt tgaacagctt tgatggcagc tttgacagtt
     9481 ggagcatcta ctacttccat ttgttctagc tgtgctttga atgcgtggag aacagtagga
     9541 actgtttcac cagcaagtac ttcttttgct gcttcattca atacaggatg ttcattgaaa
     9601 aatagcgtag acagatcaac gatttctgca gcatagctca tttgaggttg atacaggcta
     9661 acgatcttct tcacccattc gattgtcttt tcatctgggt tttctgctac acgtccatca
     9721 gcgattagat aaggcagaca catgtcagta aatgcatcta gatccattgc tttgatgtaa
     9781 tggttgttta cccattgcag ttttttcgca tcgaaagctg cgggtgattt acttaggcgt
     9841 tttggatcga acattttgat caattcttgg tgttcgaaga tttcgtcttc gcccacaggt
     9901 gaccagccca ataatgcaat aaagttgaac attgcatctg gtagatagcc taattcgcga
     9961 tattgttcga taaattgcaa gattgattcg tcacgtttgc ttagtttttt acctgtttca
    10021 ctgttgatga tcaatgtcat atgtccgaag gttggtggtg tccagccaaa tgcttcatag
    10081 atcatcagtt gttttggtgt attggcaata tggtcatctc cacgtaaaac gtgagtgatt
    10141 ttcatcaaat gatcatcgac cgcaacagca aaattataag ttggcatacc atcgcgtttt
    10201 tgtatgacaa agtcgccacc gatattatcg gattcgaacg aaatagcgcc tttcaccata
    10261 tctgtaaatg cgtattctgt attgcgaggc acacggaaac gtacaacaga ttctagacct
    10321 tgtgcttctt tttctgcttg ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga
    10381 ggcatttctc ctcttgcgcg ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa
    10441 tagcatttgt atgctcgatt gctagcaagc agctgatcaa tcagcggttg atagatttct
    10501 ttacgttcag attgacggta agggccgtat tctccaggat tttcaggaga ttcatcccaa
    10561 ttcattccca accaagcaag gttttccaac tggctttttt cgccatcttc gatattacgt
    10621 ttttggtcag tatcctcaat acgaataata aagtctccat cattgtggcg ggcaaacaaa
    10681 taattgaata atgcagttct tgcatttcca atatgcaggt gcccagttgg acttggtgca
    10741 tagcgtacgc gtactttcgt cattttcttc ctcttctttc aaactagact ttgactaaca
    10801 gcaaaaattc cgtcagttac ggcttctcaa atatcattta tttaaagcca ctcagcacaa
    10861 attttacctt ttctaaggta aatagtaaag ccaactggtt gaatttcctt accagttggc
    10921 tgaatttctt ttttattttg attgtttagt ctttttctct gtctcttttt tattgtcgat
    10981 tcctcgtcct gcatgtgcag gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc
    11041 acttgttaca acgacttcga tacgttcatt catgtaatgc tgtccatctt ctacgacaac
    11101 catcgtgccg tcatcaaggt aggctactcc ttgttggcgt tctgtcccgg cttttactac
    11161 tagaacattc atcgtttcac cagggatgac gactggtttt acggcatttg ccaacgcatt
    11221 gatattaaga acaggtacat tttggaattc agagacttta ttcaagttat agtcattggt
    11281 tacaatgaca ccatctaaaa gcttagccaa tttgatcaat ttgctgtcta cttcactgat
    11341 atcttcaaag tctccatcgt acatttcgac agatataccc tcttcttttt gcaaggcatt
    11401 taggatatct aatccgcgac gaccacgcac acgtttcaaa ctatcccctg agtccgcgat
    11461 gtattgtagt tcgtaaagaa caaaattagg gatcatcaag gttccttcta agaatccagt
    11521 ctttgtaata tcataaatcc gaccgtcaat gatcacgctt gtatccaaga ttttatattt
    11581 atggaaattt tctcctgatt ttcgttctaa cagttgactc tcaacttgac tagctgtttc
    11641 ttgctcgatt tttttgcttc gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt
    11701 tcccattctc aatcccagat agcctaagat gatcatcagt aaaatcggca atacactatt
    11761 gacgaatgga atttccatat tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc
    11821 gatgatcgca ccgatcgcac cgaagagaag ataagtcaag ctgacctcac ttagtttttg
    11881 ttctacttgt ttgataatag ctgagatatg tttggccaac agcaaagata ataaaaagaa
    11941 aataagtgcg cctaacaggc tgttggtaaa tgtattattc agccatccgt ttgactgttg
    12001 accgattgcc tgccaagctg agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat
    12061 catcagcaaa gtaatgacac gtttttgcat cgtttttcct ccgtttcgac aatcatttat
    12121 tagttagcgg aacacacgct tcaacgtttc agccaaagtt gctactccaa ctatctcgat
    12181 tccttttggc gcttcccagt tacctaagtt gtttttaggt aggtatactt ttgtgaagcc
    12241 gagtttttgg acttctttga ctcgttgttc aatatgactg acacgtctga tttctccagt
    12301 cagtccaatt tcaccgatga aacattccgt tggctgtgtt cctttttctt tatagcttga
    12361 agcaatactg acagcgatag ctaaatcaat cgctggttca tttaatttta ccccacctgc
    12421 agcttttaag taagcatctt gattctgaag aagcaaccca gctctttttt ccagcactgc
    12481 catgatcaga gatacccgat tgaaatcaag acctgttgtt gttcgtttgg catttccgaa
    12541 catagtaggt gtgactaatg cttggatctc taccaaaatt ggacgtgtcc cttccattgc
    12601 cacaacgata gcagagccag ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg
    12661 attagcgact tcttccaatc catgctcatg catttcaaaa atcccaatct cattcgttga
    12721 gccaaatcgg ttttttactg ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa
    12781 atataacacc gtatccacca tatgttctaa cattcttggg ccggcgatcg agccttcttt
    12841 ggtaacatga ccgacgataa aaatggctat cccatttgtt ttagcgattt tgagtagttc
    12901 tgcagtggtt tctcttactt ggctgacact tcctgccaca cttgtgatgt ctggctgcgt
    12961 catcgtttgg atggaatcaa tgataacata atcaggggag atatgttcga tggcacgact
    13021 gatttcgttc atatctgttt cagcgtatag ataaaattct gtgttgatac ttccgaggcg
    13081 ttccgcgcgt aattttatct gctctgcgct ttcttcgccg gatacatata agacttttcc
    13141 gcctatggct gctaattgct gagaaacttg taacagcaaa gtggatttcc cgatacctgg
    13201 atcgccacca atcaagacca tagaccctgg caccacacct ccgcctaaca cacggttcag
    13261 ttcttctaat tttgttttga tccgaggttc ttttttaggg acgacgtctg cgatttttgt
    13321 tggttttgct ttttctcctg tcaaacttgt ccgagttctg cggtccgttg tatcttgttc
    13381 gatttcttcg accatcgtat tccattttcc gcaatttggg cagcgtccca agaacttagg
    13441 cgacacatac ccgcaatttt ggcaaacaaa ttgtactttt gatttttttg ccattgtacc
    13501 gcctcctttt atcctaatca tgagacatta aatcaagctt aagtatcttt aacgttttat
    13561 ttactggaag aaccaaatcc gccttgacgt tgagcagtcg tttgatcttg atctgctttt
    13621 aaaaacggca aaaagatccc ttggccgata cgctcgcctt ttttgatcac tacatccttg
    13681 aaaccaaagt tggtgaattg gaacatgata tggccttcat ttgcctcatt attgtaataa
    13741 tcactgtcaa tcacacctac tccgtttgtc aacactaaaa accgcttcaa cggattgctt
    13801 gaacggttag caagctgcaa aaattcatct tctcccatgt aagctttgat tccagtaggc
    13861 acgagggttg gtttcaattg tttttcattt tctgatacat cttttttcaa tccatctgcg
    13921 actgtttcca accccttttt ccagcctgct ttccagatac ttggaatcac gacctcttct
    13981 gctgcttcaa aatcatagcc agcagcatga tgtgtagcgc gctgcggtag attgatattt
    14041 tgatctttat atttagaaat aacttcaaat cctctttttc tcaccggttt tcct
//
LOCUS       DACCKU010000078        14044 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.081, whole genome shotgun sequence.
ACCESSION   DACCKU010000078 DACCKU010000000
VERSION     DACCKU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 14044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.081"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IVN13_002171"
     CDS             complement(<1..132)
                     /locus_tag="IVN13_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7012638.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     "
     gene            341..715
                     /locus_tag="IVN13_002172"
     CDS             341..715
                     /locus_tag="IVN13_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7012639.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(829..1458)
                     /locus_tag="IVN13_002173"
     CDS             complement(829..1458)
                     /locus_tag="IVN13_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP7012640.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1592..1664)
                     /locus_tag="IVN13_002174"
     tRNA            complement(1592..1664)
                     /locus_tag="IVN13_002174"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1629..1631),aa:Thr,seq:ggt)
     gene            1878..1951
                     /locus_tag="IVN13_002175"
     tRNA            1878..1951
                     /locus_tag="IVN13_002175"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1912..1914,aa:Asn,seq:gtt)
     gene            1906..2055
                     /locus_tag="IVN13_002176"
     CDS             1906..2055
                     /locus_tag="IVN13_002176"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012641.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2548..3876
                     /locus_tag="IVN13_002177"
     CDS             2548..3876
                     /locus_tag="IVN13_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP7012642.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            4078..4911
                     /locus_tag="IVN13_002178"
     CDS             4078..4911
                     /locus_tag="IVN13_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP7012643.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4927..5664
                     /locus_tag="IVN13_002179"
     CDS             4927..5664
                     /locus_tag="IVN13_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP7012644.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5819..6787)
                     /locus_tag="IVN13_002180"
     CDS             complement(5819..6787)
                     /locus_tag="IVN13_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP7012645.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            7019..7852
                     /locus_tag="IVN13_002181"
     CDS             7019..7852
                     /locus_tag="IVN13_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP7012646.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7886..8725
                     /locus_tag="IVN13_002182"
     CDS             7886..8725
                     /locus_tag="IVN13_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7012647.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8751..9311
                     /locus_tag="IVN13_002183"
     CDS             8751..9311
                     /locus_tag="IVN13_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP7012648.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9510..11231
                     /locus_tag="IVN13_002184"
     CDS             9510..11231
                     /locus_tag="IVN13_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP7012649.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11396..12538
                     /locus_tag="IVN13_002185"
     CDS             11396..12538
                     /locus_tag="IVN13_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP7012650.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12554..13765
                     /gene="thiI"
                     /locus_tag="IVN13_002186"
     CDS             12554..13765
                     /gene="thiI"
                     /locus_tag="IVN13_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP7012651.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4781 a         2327 c         2850 g         4086 t
ORIGIN      
        1 ccatttgtca aatgctgaag ccgttagttc atattctcga atgatttcta cacgtggctt
       61 tccagctaag taaagattga cgatttgttg cttgaattct tgtgaaaaag ttcttcgtgt
      121 tctcttagac ataaaaattc ctcctggtat gttttcttct agtctacaca ccttaatttt
      181 tctgtctagt taattgtagc ctatccaagt ctacactctg agatgtaaga aatatcataa
      241 gatatttctt acatcttttt gttttgagca ggactttaga taattttatt aatatatgct
      301 agcatgcttt tgtaagaaaa agaaagaggg cgtattagtt atgtatctta aaattacgga
      361 acaagcacaa gaaagattac aaaaatatat tgatgaaggc gcaacagtta tattggattt
      421 agatgatggc gttggggaat attctaaaat gggtgtttgc tctttagata ctagttttcg
      481 tttacttctt cttgataaag aacagagaaa aaatgattat aaactccaat tagatagtga
      541 tattggggat gtttatataa aagattattc aaaaatgtac atggatgaac atatgaccct
      601 ttctttagat ccacgtctag gtgtatttac tcttgaaagc ccaagtggtt ctttagacag
      661 tcatgttcaa ttagtagatc tacgaccaga aaaattctct ggaataaaac aataactttc
      721 tctctattgt ccataaaact agtctcaatc aagctgtatt gaaaaaacaa gacaacattc
      781 ttaccatggt aaataaaaag acgacacatt ttgtcgtctt tttttaaatt aactattttc
      841 gataaaagat cgcatagctt gtgccattat ttctggcttt tctctattca attcatgttt
      901 tccgttttta ataaagagta gccggccatt cgttgaaagt ttatataaac gttttgctgc
      961 tggtttattc gcatgatctt tggtaccgca taaaatcaat ttaggaagtc gtgatgtacg
     1021 aagctgtttt ttgcagtcta atgtttcaag tgaacgaagt agggtaaggg tttgtttttt
     1081 agatagacct aatttttcaa atatcttaga aggtacgcag taaaaataaa tattctggaa
     1141 agtaagcaat aagcgattag gagactcaaa ttgtggagcg actaaaacaa ccttttttaa
     1201 atatggagaa ggagttgcaa gatagtttaa agcaataaca gcgcctaaag ataatccaca
     1261 taaaataaaa ggttccttta tctgattcaa ttgtacttcg atgtatttgg ttaagtcgtc
     1321 taaagtagca gtgtctgtgc tgtttaaatg attgaacaat gaaagacttt tgcaagaata
     1381 actattgggt agataagaga ttacatcttt ccatgattgt tcgtcttggc ctagaccatg
     1441 caaaaaaaca attttcatat tgctctcctt tttatagtta tcatatagac tttctttgta
     1501 ctattaaaat ataaacattc atttcttacg tcataaaata aaaaaacagc gcagacacat
     1561 tgttaaacca acatgttgca ctgtttaagg aagccacttg ccgggcttga accggcgacc
     1621 tcttccttac catggaagcg ctctaccagc tgagctaaag cggcattatc attaatacaa
     1681 ataataggat aacgaatatt aatgtatttg tcaacagaat tttttcgaaa aaatgtgttt
     1741 ctctaatagg aaaacagatt ttagaaaaaa taaaaataac aaaaaatgcg aacggtttct
     1801 ttgataatat aataaaaaaa ttcaaaaaaa gtattgcgtt atttagaaga aattgatatt
     1861 atattcatgt acttgattcc ggcatagctc agttggtagt agcgcatgac tgttaatcat
     1921 gatgtcgtag gttcgagtcc tactgccgga gtaaataaag cttatgcaaa gaaaaagaaa
     1981 tccaatcact tgggtttctt tttcttttat tattatgatg aatcaagctt tctgtttttg
     2041 tcacgtatgt tgtaatcttt acgaaagagt agagttggct actcataatc aaccatactt
     2101 cccatcatcg ctaaatacga aataaatcag cattcctaca ccaatcaaag cacaagctgc
     2161 accaagaaaa gggatgacat tcccaaagtt atctgtattc ataagtatgc caccacccac
     2221 aaacaaaaag ccgccagcaa tcaggttttt cattttattc atctcctttc ttacgttata
     2281 gtatttcctt ctttcaatga gtattttgaa agctatctta ttgtatgatg ttcttctagg
     2341 cctcttcttt attatacagg ccattttcgt tatctcatag aaatattcct ttttatctga
     2401 aaactattgt ataccgtatg acatttttct tcatgaagtt gtaagtatat tctcattcaa
     2461 atgaaaattt catcatagaa gagattgtat ttctaaattc ttaagagtaa aatacgaagt
     2521 ataacttgga tttggaagga tgattggatg aaagctatta cgcctgatat gacaacaaaa
     2581 tttaacaaac agtttcaagc aaatatcaaa cagcaggcat tgcagcgggc agttgtgaag
     2641 aacggaatta ctgcttctgc tgaaaatgtt tcagcacatg tggagaatac accggtattt
     2701 tctatagatt tggctacggg gaaagtggct aatcaaatgc aatcggggcg ttgctggatg
     2761 tttgctgcac tgaacacatt ccgtcataag atgctgaatc aattccattt aaaagagttt
     2821 gaattatcac aaaattacac ttttttctgg gataaatatg aaaaggcaaa ttatttctat
     2881 gaaaacatca ttgcaacagg aaaagaggca atcgatagcc ggaaagtagc tttcttactc
     2941 gctactcctc aacaagatgg cggccaatgg gatatgatcg tttcattatt ccaaaaatat
     3001 ggcgttgtgc ctaaatcggt gatgccggaa agtgcgaata gttcaaattc tcgagatttg
     3061 aataactact tgaacaaatt actgcgtaaa gatgcagtag tgttacggaa aatggttgct
     3121 caaggagaaa cattagacgc tattgaagaa aagaaagaag aaatgctaga aagcatttat
     3181 aacttcttag ctatctcatt aggaacacct ccaaaagaat tcgattttga gtaccgcgat
     3241 gaagaaaaga actatcatct tgaccgagga ctgacaccac aaatttttta tgataaatac
     3301 atcggtgtaa aattagatga ttatgttagc gtgatcaatg caccaacaaa agacaaacca
     3361 ttcaatcgtt cttatacagt agagatgcta ggaaatgtcg ttggcgggaa agaagtcaaa
     3421 tatctgaatg ttgatatgga aactttcaaa aaattagcga ttgctcagct tgaagaagga
     3481 gaatctgttt ggtttggttg tgatgtaggt caatcttcta ctagaactag tggcatcatg
     3541 gccttagatg cttattcaat ggatgatcta tttgatacgg attttacaat gactaaagct
     3601 gaacgtttag attttggaga aagtctgatg acccatgcga tggtattgac cggtgttgac
     3661 ttagtagacg ggcaatcaac aaaatggaaa gtcgaaaata gctggggcga aaaagttgga
     3721 aagaatggct tctttgttat gagtgatgcc tggatggacg aatatacgta ccaaatcgtt
     3781 gtaagaaaag atttattgac gaaagagcaa ttaaatgcgt tcaatgaaga accaattgtt
     3841 ctatctccgt gggatccaat gggtgcttta gcatagacga gtatttcaat gaagagtatg
     3901 tagtaaagat aaaaagagct ggcttaagcc agctcttttt atctttaaag tccattccct
     3961 gttttttatg gtccctgtgc tataattcta aataaaggat aaggattgga aataatcctt
     4021 ttatcattaa aaatatgatg acgaagtaga ataaaattgg gagtaggggg gaaagtcatg
     4081 gatatccaaa ctgttttatc tgatctgaaa ttaaatggta aagtgattcc agttgttggt
     4141 ggcgatgtta atcagaccta tcgtataaaa accgaacatc gtgcttattt tttaaaaatc
     4201 catccgaatg taaaaaaagg attttttgaa gcagaagtag acggattaaa agaactgtca
     4261 gcattcgtac gtgtaccgga tacgtatatg ctaggtgaaa caagtgaagg cgcttatttg
     4321 ttaatggaat ggatcgagcc gggaaaaggg gatcaacgag atcttgctgc agctttagct
     4381 aatctccatc agcaaacagc gccacaattt ggttttcgta aagataacta tttgggtaca
     4441 cttgttcaga aaaattcttt tgaagaagat tggtggactt ttttctttaa agatcggctt
     4501 gaatcccaga tttctttggc agaagaaacg aatcgatgga atgtacagcg tcaagaaaag
     4561 tatctgcgat ttaaagaacg tgtattgaaa tcggttgaac caaaaaagat cactccacgc
     4621 ctcttgcacg gagacctgtg gagtggtaat gtgttctttg accagcaagg gcatccagtt
     4681 tttgtcgacc cagctgtttc ttatgggaat cgggaacaag atattgcaat gagccagtta
     4741 ttcggtggtt tccgaccgga atttttagat gcttatcaaa ctatatttcc attagaaaag
     4801 ggatggaaag accgactgcc gatttaccag ttatattatc ttttggcaca tttgaatatg
     4861 tttggagaaa gttacggttc ccaagtagat caattactag aaaattttta agaggagggc
     4921 gtgtttttgg caaaaaaata taccaaaaag catgaagttt catattatga gtgtgacatc
     4981 aaccaaacga tgacatttcc ttcgatgtta ggagtagtta tcaaaacgtc tgaagatcaa
     5041 agcgaccagt tgggaagagg cagtgaattc gtcaatagct ttggacttac ttgggtcatc
     5101 acgaattatt ccatggagat taatcgtctc cctagagtcg gtgaagtaat ctcagtaacg
     5161 actcaagcca tggagtacaa taagtttttc tgttatcgga acttctggat ccatgatgaa
     5221 gatgggaaag aattagtcaa aatagaatct gttttcgtat tgatggattt tgtgaatcga
     5281 aaaatgagca gtgttaatga agagatcatt gctccttttg agagtgagaa gatcaagaaa
     5341 atcaagcgtc aggaaaaaat tgaaaagatt gattcgggct tcatgttgcc atatcgtgtt
     5401 cgtttttatg atatcgatag taatcagcat gttaataatg cgatgtattt taattggatc
     5461 attgacgtat taggatatga ctttttgact acgcatgttc cagaaaaagt aattattcga
     5521 tttgataaag aagtagagta cgggaatgag atcgaaagcc attttgaaca aatccctatc
     5581 gaaaatggtg tcaaaacaaa acatgaaatc cgaatgcaag aacagctgta ttgtgaagca
     5641 aatattatat ggaaaaataa ataaaaaaga ggttgtgaca gaagggggca acttcaagaa
     5701 ataagaagaa atttacgaaa attgtttctc aaatttttgt aaattttcga cttagttttc
     5761 gaagaagcta tttttgtcac agcctctctt tttaagttta gctattagat aatgatgttt
     5821 agttttggat aaacgaaatc aattcttcac ccgacaaacc atagtttaca gcaatcaata
     5881 gttttaaacg tgctttttga ctattaatac tgttacagaa aataatcccc attttttcta
     5941 gttcttttcc cccgccttca taatcataaa caggctctgc gattccattg aaacaacgag
     6001 aaacaagtac tacagggatt ttttggacaa gaagcttttc caaagcagct aaggcttgtg
     6061 gagggagatt ccccgcacct aaagcttcga ttaccaaacc gtctactttc gtatgggcga
     6121 tggcttccaa taagtcccct tgcatcccag catatgcttt aacgatcggg atagtcccat
     6181 ctacagcgga aatgtccagt cgctttgttt ctaaaagttc ctgcaagaat aaaattcgat
     6241 tcttagttac taagccgatg ggacccaatg taggtgttcg gaaagtagca acatttgtgg
     6301 tatgggtctt tgtcacgtag cgtgcagaat ggatctcatc gttcatgacc accaaaaccc
     6361 ccttatctag cgcttcttca caacttgcga cacgaatcgc actttcaaaa ttataaaggc
     6421 cgtcacttcc aagttcatta cttgaacgca ttgccccagt caaaacaatt ggtaatttgt
     6481 tgccaatcgt tgtatcaaga aaaaaagcag tttcttctaa tgtatctgta ccgtgtgtga
     6541 tcaccactcc ggatatacct gatgcataag cggacaagat acgttctttt aataccaaca
     6601 tgtgagaagg catgatgtgt ggtgagggaa gattgaagat atcttcaact aataattcta
     6661 catgcttggg gattgtgatt tctgcatcta ataatgggtt tgccacatca ggactaacac
     6721 cgcccgtgag catgttttct ttcatggcga tcgttccgcc ggtgtgtagt acaagtattt
     6781 ttttcatttt acagacttcc ttacgaataa ttttgcatag ccgttaaatc ttattgtata
     6841 ctatataaaa gagttttgct atcagtgaag aataacgaat agcctaactt atcataaaaa
     6901 agctgacctt attaaaatgg aaaagactca accttgacct cctaatacaa tggagtatct
     6961 ggttgagttt cttttacaag aaagcagcca aggaaatcaa tcacgaaggt ggccggaaat
     7021 gatcagaatc ggattaacat cgtttaaaga acatgacaaa ttaactggta aaaagaataa
     7081 cactctttat gaatatgcag ggtttctacc cttagtggaa ttagatactg cctattatgg
     7141 aattccccga aagtcctcag ttgagaattg gataagtgaa gtacctgaat catttcgctt
     7201 tgtagtcaaa gtctatcaag gaatcagcgg tcaaggagac tggaagcctt attatcagac
     7261 cgaagaagag atgattgcgc attttttaga tgttatgtcg ccaatggtag atgctggaaa
     7321 gatgttttgc tttttagtcc aattcccctc atcattcaaa tgtacgaaag aaaatgtcca
     7381 gtacttgcgc aaacttagaa agtggtttga gggttatcct gtttctatag aattgcggga
     7441 ttattcttgg tacagcgatg aatttcttga acgtacaaga aactttatgt ctagagccgc
     7501 tttttcttta gccatcatcg atgagccaca gttgctgaat acaacgattc cttttgatcc
     7561 atttgtcacc aatccgaact ttgctctttt tcgattccat ggtaggaatg cgcaaggatg
     7621 gcaagcaaat gataaagagt ggcgtaaaaa aagaacgtta tatcgttatt cagaaaaaga
     7681 actgcaagaa ctagcacatg aagtcaaagt agttgattct aagacaaaag aggtaggtgt
     7741 gatttttaat aataattctg gtggtgatgc ggctgaaaat gcattagcac tgcaaaaaat
     7801 attaaatatc aagccagatg gactcaatcc aacccaaatg gatctgtttt aagggcttat
     7861 tttattcaag aaaggggcga agtctatgat caaagctatt ttttttgata ttgacggaac
     7921 attggtcacc aatcgttcaa aagcgttgga atcaacaaaa caagcaattg aatatgctcg
     7981 gaaaaacggc attttatgcg gtgtagcaac ggggcgcagc ccagtaaaga tcaaagaaat
     8041 cattgatgaa ttggaattag atatgtatgt cgtgtataat ggtcaactgg tttttactgc
     8101 agatcgcacg attatcgacc acccttttga acaaaaagta ttggaacata ttgttgaatt
     8161 tgcagatgag aaccatcgac aaatcgtttt tggtgcgaga aatcggttag atggatcaac
     8221 gacgatgcta ttaggacaat cgatttttat caagcgacta gtcagctttt taccgcgtaa
     8281 attcccggta cgtctgatga aaaaaatcct ccaggttttt agccctcacc gacaaaagga
     8341 tcgttatgaa acgttagaga tattaaaaga accaatttat caatgtatat tgctcagtcc
     8401 ggagtcagaa cagaaaaagt tagaacatcg ctttcccgag tgtacgtttc agcgttcaaa
     8461 tacgtatact gtagatatca tcccaaaagg tggttcaaaa ttactaggca tccaagcttt
     8521 cgcaaatgct gtcgaaattg aaatggaaga aattatggca ttcggcgatc attacaatga
     8581 tatcgagatg ctgaaaggtg taggaattgg agtggccatg gggaatgctc agatcgaagt
     8641 aaaacatgct gcagactttg tgactcaatc aaatgaagaa gatgggatat acgaaggact
     8701 gaaacacttc ggtctgattt attagtcaat gaaagaagga aaaaatcagt atggaagcag
     8761 agacaccttt tcaaaaagta gaaacattcc acaaaacttt tgatccaaga agaccagaga
     8821 tacccacggc ttttacagaa aatgaagctc atacacgtgc tgcgttcaaa atagaagaac
     8881 ttgttgaatt tgttgcagct acttcaccta ataaagaaca actgcatgaa gctgttatgc
     8941 tgctgcacga agcattagat aaagcagaag aaaaaatact tggtaaaaca cagtggggaa
     9001 gtgatccgct ggttgaacaa gtagatgcac tgacagatat cctttacttt acttatggtt
     9061 ctttttcatt actaggaata gtgcctgatc gaatattcga aatcgtccat caggcaaata
     9121 tggggaaatt atttccagat ggaaaacctc attatgaccc tgtaacaaat aaagtgttga
     9181 aacctgaaaa ctggcagatg gaatatgctc cagaacctaa aatcgcggca gaaatccaac
     9241 gtcagattca agaagcgatt gctaagaaga aatccgctga tataggcatg tgcacaaatg
     9301 aaaaagagtg atcagagttt cactattttt gttatcttta tacgatggaa aaacaaaaga
     9361 ttttcgttta agttagtgaa tgcatgaaaa tagtatgcta taatctagaa tagatgtctt
     9421 ttgcacgaga gacgtattat ttggatttat gccaagcaga acgccatctg ttgcataaaa
     9481 gatgcatact gaaaacggag gaaccataaa tgggaaataa tgttatcatc ggaatcgtca
     9541 ttgcaatcat tatagtcgcc attgtcgtct ttttggttag ttacttgatg aagaaaaaaa
     9601 accaagatcg attggatgcc ttagaaaaac gcaaggaaga attatttgac ttgccagtga
     9661 ttgaagaagt cgatgatgtt aaaaaaatgc atctggttgg gcaaagccaa aatacctttc
     9721 gtgaatggag tcaaaaatgg aatgacatat ctacttcctc gttcgctgaa ctagaaagtc
     9781 aaattttcga agtggaaaac ctcaatgaaa gttttcggtt cttaaaagga aaaaaagcag
     9841 ttgttgaagc agaagctaca atggaccaaa tggagaaaca agtgacggaa atccgtgaag
     9901 gtctaaagga attgcgggaa acggaagaac gcaactcact agctgttcag caggcacttg
     9961 acgtctatga agaactgaaa gaaaaagtca gagaagacga gcatgaatac ggaccagctc
    10021 tttttgaatt gcaaaaacag atcaaagtga tcgaaaaaga atttacgcag tttgtgactt
    10081 taaacacttc tggagaccct gttgaagccc gcgaggtttt ggataaagca gaaaaacata
    10141 cgtatgaagt ggaagctctg atgaaaaaaa ttccaccgct gtatgaggag ctccatacca
    10201 ctttccctga acaacttgaa gaaatcagtg atacttacga gaaattgatg gaagaacaat
    10261 atgtattccc agaagaagat cttgctgaag atatcgcaaa agtaagtcga agaattgaaa
    10321 attcattagc caatcttgaa aaaacagaag ttgaaacggt cgaatttgaa aaccgtgaaa
    10381 cagctgattt gattgattcg ttatatgata tcttagaacg ggaaatggaa gctcagagat
    10441 atgtaaaaac taaccaatca acgattgcag agtatatcaa acatacaaca aagaataacc
    10501 gtcaattatt gattgagctg gatcacacat ctcaaagcta tacattgaac cacaatgaaa
    10561 ttggccgtgt acgaggtttc caaaccgaag tggaagaaat ggaacgtcaa aatgaacaga
    10621 tgattccaca gatccgtcaa cacgaaattc cttattcaga agtgcgcacg ttctataaaa
    10681 cagtcttcaa agtcttagaa gatatcgaaa agcaacaagt agagatcgac gactcattac
    10741 atgaattgag aaaaggcgaa aaagaagcgc aagaaaagat cgatacattc gaattcaaat
    10801 tgcgcagttt gaaacgtttt gtagaaaaac aacgtttgcc tggcttgccg aatgattacc
    10861 tagaattctt ttttgttgct actgaccgta ttgaagaact aagtgttgtc ttgaataaaa
    10921 ttcgagtaaa catggaagag gtcaatcgtt tagttgctct atgtgaagaa gatctagaat
    10981 tattagataa gaagacacat gatttagtag atgcagcagc attaacggag caaatgctcc
    11041 aatatgcaaa tcgttatcgt catacacacg aagaagtgag agaagcaatt gataaaagct
    11101 actatttatt caataaagaa tatcactatc aagaagcatt agacgaaatc ggtacagcat
    11161 tagagcgagt agaacctggt gctttcaaac gcatcgaaga tttttatttt aatcatccag
    11221 acttagtata aagcaaaaaa gttagtaaac cagcaagagc aagacggatt attgtcttgt
    11281 tcttgtcttt ttttgtatat ttgaaaattg aaacaagtga aaatttcacc tgctcttgaa
    11341 agcaccttgg acaaccgtta taatagaaaa gaatttcaaa aaagaggggt ttcttatgat
    11401 atattttgat aacagtgcaa caaccccaat ctttccgcaa agcttagatg catatgtaaa
    11461 aaccagtcag cggattattg ggaatccatc aagcttgcat gatttaggca atcaagctaa
    11521 ccggttgttg cagcaagcaa gaaagcagat cgctgatttg atccatgtag aaccagaaga
    11581 aatctatttt acttcaggcg gaacagaagg agataactgg gtattaaaag gaacgatgat
    11641 cgaaaaaaga gaatatggga atcatatgat catttcagga gtcgaacatc cggccgtcag
    11701 cgagacagca gaacaattaa aagaattagg aatcgaagta agtatcgccc cagtggataa
    11761 acgcggattt gtacgtgtcg acgaattgaa agaattaata agaaaagaga cagtattagt
    11821 ctctgttatg gctgtgaata atgaagtagg atcgattcag cccatccagg aaatcagtga
    11881 tcttttggat gattttccta aaattcattt ccatgtagat gctgttcagg cgattggaaa
    11941 agtaccattc tcgcaatggt tgacagaaag agttgatttt gcaacatttt cagctcataa
    12001 attccatggc ccacggggaa ctggtttcat ttattggaaa aagggtcgtc gtttagcacc
    12061 attactgaca ggtggagggc aagaaagaaa tcagcgaagt gggacagaaa atgttccagg
    12121 tattgtggct atggttaaag ctttacgttt atcattagag ataaaggaac aaaaacctga
    12181 gcagacacaa cgcttaaaag tagctctaat caatgcgttg aagacgtatg agaaagtgac
    12241 gattttttca gaaggaactg actatgcacc gcatatttta tgttttgctc taaaaggaat
    12301 tcgcggcgaa gttctcgtcc atgccttaga agaaaaacaa atatttgttt ctactactag
    12361 tgcctgctcc tcacgtaaac atatggcaag cagtacattg cacgcaatgc atgtaccaaa
    12421 tgatctggct acatctgctg ttcgaatcag cttagatgaa aacaattcgc ttgcagaagt
    12481 cgagcaattt atgattattt tcaagcagct ttataagaaa ttttctgcaa tcaattaaaa
    12541 gaggagtgtt cttgtgaaat acagtgaaat tatggtccgt tatggggaac tttcgacaaa
    12601 aggaaagaat cgaaaaagct ttatcatgca actagcacaa aatgtcagac aagctttgaa
    12661 agactttcca gaaatcaaaa tccatgctga tcgggaccgg atgcatcttt tattgaatgg
    12721 cgcagacggg cagctagtta ttccaaaact agcaaaaata tttgggatcc aaaatttctc
    12781 tccaagtatt cgtgtagaaa aagatgtcga agttttgaaa caagctgttc aagacatcat
    12841 gaaagaaatt tatactggga aagaaacatt taaaattatc gctaaaagaa gcgatcacca
    12901 atttgaattg acaagtaatg aattgaatca aacattaggt aatgctgttt ttgatatttt
    12961 cccgcatatc caagttcaaa tgcgtcaacc agatattcca ttacgtgtag agattcggcg
    13021 tgatggcgct tatctatcgt atgaaacgat caagggagct ggaggattgc ccgtaggaac
    13081 aagcggaaaa ggaatgctta tgctttctgg ggggattgat tctcctgtag cagggtactt
    13141 tgccatgaag cggggagtgg aaattgaagc tgttcatttt gctagtccgc cgtatacaag
    13201 tgaacaagca ctgcaaaaag caaaagatct aacagccaaa ctagcacctt atgtgggaac
    13261 gatccatttt atcgaagtgc catttaccga gattcaagaa gaaatcaaaa agtcagtgcc
    13321 acaaggatat tggatgacaa ttactcgtcg aatgatgttg cgtttgaccg atgaaattcg
    13381 ccgtattcgt catggtttag tcattttgaa tggtgaatca ttaggtcaag tggcttcaca
    13441 aactttgcaa agtatggtgg ctatcaatga agtaacgaat acgccaatca ttcggcctgt
    13501 agcgacaatg gataaacttg aaatcattga agtagcagaa cagatcgata catttaatct
    13561 ttctatccag ccttttgaag attgctgtac gatttttgct cctccacagc caaaaacacg
    13621 ccctcaactg gaaaaggtcc atcaatatga agagcgttta gcaatagatg aaatgatcca
    13681 acgagcacta gctggcttga agatggaaga gatacaagca gaatcgtcta atgaaaaaac
    13741 tgaagaattt tcagaattgc tataagaggt tatgaaaata gtgttttgac cagagtatta
    13801 gagaaacaaa acgaaaaaat agctccatgt attttaccgt ttttgaacta atgtcgaaga
    13861 ttagctgatt gaataccatt tatcaagaag ttatggaaaa gccggttctt ttttactaac
    13921 tgaaaaataa aattttattt agagatcaag ctacagttga taaatgatat aaaaaagaga
    13981 atttcccatg aaatagcgag gttgtgacag aagtgggcag cttcaagaaa taagaaggaa
    14041 ttta
//
LOCUS       DACCKU010000079        13910 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.082, whole genome shotgun sequence.
ACCESSION   DACCKU010000079 DACCKU010000000
VERSION     DACCKU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.082"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IVN13_002187"
     CDS             complement(99..1229)
                     /locus_tag="IVN13_002187"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP7012652.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IVN13_002188"
     CDS             complement(1878..3080)
                     /locus_tag="IVN13_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7012653.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IVN13_002189"
     CDS             complement(3140..3343)
                     /locus_tag="IVN13_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7012654.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IVN13_002190"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IVN13_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IVN13_002191"
     CDS             4880..5692
                     /locus_tag="IVN13_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7012655.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IVN13_002192"
     CDS             complement(6716..6868)
                     /locus_tag="IVN13_002192"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012656.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IVN13_002193"
     CDS             complement(7059..7937)
                     /locus_tag="IVN13_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7012657.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IVN13_002194"
     CDS             complement(7976..9094)
                     /locus_tag="IVN13_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP7012658.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IVN13_002195"
     CDS             complement(9091..11190)
                     /locus_tag="IVN13_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP7012659.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IVN13_002196"
     CDS             complement(11241..12062)
                     /locus_tag="IVN13_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7012660.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IVN13_002197"
     CDS             complement(12059..12868)
                     /locus_tag="IVN13_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012661.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IVN13_002198"
     CDS             complement(12889..13383)
                     /locus_tag="IVN13_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012662.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IVN13_002199"
     CDS             complement(13395..13883)
                     /locus_tag="IVN13_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7012663.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
BASE COUNT         4222 a         3016 c         2248 g         4424 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa
//
LOCUS       DACCKU010000080        13858 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.083, whole genome shotgun sequence.
ACCESSION   DACCKU010000080 DACCKU010000000
VERSION     DACCKU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.083"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1299)
                     /locus_tag="IVN13_002200"
     CDS             complement(<1..1299)
                     /locus_tag="IVN13_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP7012664.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIF"
     gene            complement(1296..1949)
                     /locus_tag="IVN13_002201"
     CDS             complement(1296..1949)
                     /locus_tag="IVN13_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP7012665.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2237..3170
                     /locus_tag="IVN13_002202"
                     /pseudo
     CDS             2237..3170
                     /locus_tag="IVN13_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(3078..3794)
                     /locus_tag="IVN13_002203"
     CDS             complement(3078..3794)
                     /locus_tag="IVN13_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAP7012666.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3844..4053)
                     /locus_tag="IVN13_002204"
     CDS             complement(3844..4053)
                     /locus_tag="IVN13_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012667.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            4330..5603
                     /locus_tag="IVN13_002205"
                     /pseudo
     CDS             4330..5603
                     /locus_tag="IVN13_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5710..6741)
                     /locus_tag="IVN13_002206"
     CDS             complement(5710..6741)
                     /locus_tag="IVN13_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012668.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6748..7578)
                     /locus_tag="IVN13_002207"
     CDS             complement(6748..7578)
                     /locus_tag="IVN13_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012669.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7575..8381)
                     /locus_tag="IVN13_002208"
     CDS             complement(7575..8381)
                     /locus_tag="IVN13_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7012670.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8387..9211)
                     /locus_tag="IVN13_002209"
     CDS             complement(8387..9211)
                     /locus_tag="IVN13_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAP7012671.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(9201..10301)
                     /locus_tag="IVN13_002210"
     CDS             complement(9201..10301)
                     /locus_tag="IVN13_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7012672.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10479..11264
                     /locus_tag="IVN13_002211"
     CDS             10479..11264
                     /locus_tag="IVN13_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAP7012673.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            11297..11680
                     /locus_tag="IVN13_002212"
     CDS             11297..11680
                     /locus_tag="IVN13_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7012674.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11756..11887
                     /locus_tag="IVN13_002213"
     CDS             11756..11887
                     /locus_tag="IVN13_002213"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7012675.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11856..12092
                     /locus_tag="IVN13_002214"
     CDS             11856..12092
                     /locus_tag="IVN13_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7012676.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(12170..13141)
                     /locus_tag="IVN13_002215"
     CDS             complement(12170..13141)
                     /locus_tag="IVN13_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP7012677.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4198 a         3024 c         2419 g         4217 t
ORIGIN      
        1 aaagataaaa aattcttcat cctgcaatac tttatattct atattgtaat tattcagaat
       61 attacttaca atatataaac ctaatccatt actattttcc ttatttaaat caaatttaac
      121 atcaaatatt ttatccatat tcgaaatttt attgttaccg tatgaattct ctatatataa
      181 ccaatcatta actatcccaa tattaattac cccatttaca tcagtatatt ttaccgcatt
      241 actaatcaaa ttagatagaa taatctttaa agctgttttt cctatataaa cattctcgtc
      301 taatatataa ttattgacag ttattttctt ttgatttgct aatatttcat acttttttaa
      361 tatgtcttct aatgtatcat ttattttcaa atattcttca tcattattca aattttctat
      421 agaatgaact gataatattt gagaaatatt ttttgttaaa ctatcaacga tttcaataca
      481 ttcattaata taaatatctc tttctttata ttttccaata ttgtatttca tgttttccag
      541 tattatttta agactagcaa gaggggtttt aagttcatgg gatgcacctt taaaaaagtc
      601 atattttaac ttttctaatt ttaaaatttc tttattttta aattccaaat cgtcaattgt
      661 tcttaataaa gtagaataca aatcattaat ttgttgtttt aattctccaa cttcatcatt
      721 agagctaact tttaaactca tttttttatc aagtttcatc attttatcag taacaatttt
      781 tatttcttgt atattatttt ttattgattt tgcataaatt aaagaaataa tagcagaaaa
      841 taaaattgat attaataaag aatatggtaa aaatttaagg cttaaatctt tcgcatcttt
      901 ttgcatatca gctgtagaaa caaattgtag atttgttttt ttaccatcat taagctttat
      961 ttctctttct tcaattatca aagaattact atcactttct aaattaacat ttatattatc
     1021 tttgatttgt atttcattct tattattttt ttcttttatg aatactttta tttcactact
     1081 cttggaataa aactccaaag tttgttctat atattttatt tcttttccat tcatattact
     1141 agaaatttca tttgccatat tatgaatctt ttcttttctc gtctccaaat atgttttagg
     1201 aaagataaaa aaaattaatg aatgaactaa tataattatt attccaagaa cagaaaatat
     1261 ttgtatgaac atttttggaa atatctttaa ttttttcatt ttctctccaa tttatatcct
     1321 acatttctta tagtagttat acagtctaat tgtaattttt ttctaagttc ttttatatat
     1381 acatctatta ctcgatcata aggagtttct tcgctatctt tccatacata atcaataatt
     1441 tgcattcttg ttaacacttg tccattatta tccaataaac attttaatac ttctagttct
     1501 tttgcattta catctatttc ttcaccattt attttagctg tatagctatt aaaattaacc
     1561 gatagatcct tatattcaaa cttctctaaa tgtccataat tcttcttaat taaagaatct
     1621 attctagctt ttaagaccgg caatgaaaat ggcttttcta catatccgtc tactaaatta
     1681 gtaaatgcat caattttata ttcttcatca ctaaatgcag tcaagattag aattggtaaa
     1741 ttgcttttct ttctaatttc tttcaacact tctaaaccat ttataaaagg tatctgaatg
     1801 tctaagataa ccaaatttat atcgctatta aattttgaca aggcttctct tccatcttta
     1861 gcttgaataa cagtatatcc aaattctgaa agatattcac ttattccctc tcttatcata
     1921 ttatcatctt caacagttaa tattttcata tatacctcca cttaagccta tacactagat
     1981 attatacagc atattatggc tctataatat ctagtgttgg tgacgccttc tgttgccaat
     2041 actttttctt ttttttaata aatgcaaaca gacaccttga aatcctttag aaaaaaggct
     2101 tctataatat tttgtgttgt tggaaaatcg aaggcgattt tctactaaca ctttttcttc
     2161 gtttcaactc aaaaagagac tcacttttgg taaaatttag tcgaccaaaa catcacccta
     2221 ccaaaggagt cgctctatgg ataatcatac tagaaaatta ctcggattaa cagataaaca
     2281 tttatttttt gatgaagaat ggctgattga aaaagaatat aaaggcgttc aagcgcagtt
     2341 tattaaaggg agattggatg actcaccttc ccattgtgaa cactgtggat cgattcgtat
     2401 tatccgcaac ggttcctata cgacacgtac tcagatgttg aaagtcaaag aaaagttgac
     2461 tattttagaa ttaaaacgta cccgttttct atgctatgat tgtgggcaga ccttttctgc
     2521 gaaaacggac ttagttgatg aacatcatca actaacaaag gaactgaagc aggcgattct
     2581 catagaactg tacgaaaatc aatcacgtaa actaatcgct aagaagtact tcgtctcgga
     2641 tgggacggtg acacggatct tgcgtgaagc aacgaagcat taccagccac gtatgaactt
     2701 ccttccgacg gtgttgtgta tggatgagtt caaatcaatg aagtctgttt ctagttccat
     2761 gagttttatt tgtgttgatg ggacaaccca tcagctattt acgatccttg aagatcgcag
     2821 actttacaaa ctaactcaat acttcctgca ctttcctaga aaagcccgtt taaaggtcaa
     2881 gtacctagaa tgccagctat tgtcagctgc ttaaaacggt ctttccaaat gcggagatcg
     2941 taactgatcg ctttcatatt gtccaacata tcaatcgcag cttcaatcaa ttgcgtatcc
     3001 agatcatgaa tcgttttcga gcctcgcact ctgaaaatca gaagaagtac cgtcggctca
     3061 agcgatattg gaaactctta ctcaaagatt ccacgacatt ggagccgctc aaacggcatt
     3121 atcatcgact gtttaaacga ccaattagtc aaacagaaat cgtcgatgaa ccgttgtgca
     3181 caatggtgtc ctgaacgtac agcgacacct tccatatcca gagcagtcgc cacatcacgg
     3241 ggatggatgc catccaaatt aaaagcaacc acgccagtat gatgttcagg atcttgcggt
     3301 ccgtaaatgg tgataccgcc gatcgcttgt aatttaggta aaacgtaggc aaccagttct
     3361 tgctcataac gatgaatatt ctccatacca atctcattca agtaatcgat tgctcctgca
     3421 atgttcggag taccagcttc aaatttccat ggcagctctt tccatgaact atcgtaaaga
     3481 ttgacaaaat ctatcatttc accgccgaat tcgactggct ccatttgttc taacaattga
     3541 cgtttgccat ataatacacc gattcctgtc ggcccgcaca ttttatgccc gctaaaggcg
     3601 aaaaaatctg catccaacgc ttgtacatca actgtcatat gcggtgctgc ttgggcaccg
     3661 actggactat cctcaactac aacagcctgt tcaaccctca gccgtttcaa gcacaacaaa
     3721 taaccttctg gatctggttt gccgacctga agatcatcga tcgtcacaaa ggaatcaatt
     3781 ttttccttaa tcatcggatg tgcttcaaaa aaatagtcta gctcccgcag ttcagaattg
     3841 agactactcc gattttccag tgctgctgat ccgctgctca aaatgttcag catccactgg
     3901 tctcccaacc gcttgactta tcccttcata aaaggacagt tctgctgccg tcccaacacc
     3961 ttgttgatag tctgcaaaag gaattttata gccgaactgc tccagtaaat aggcttcagt
     4021 aaatagtgat gaatactctc gtaaaacatc cattcactgt caatcatcgt cccatcaaaa
     4081 tcaaaaataa gtgctttggt catcttcttt cccccttacc caaaacaagc aaaagctgtg
     4141 tcctgctctc tctacagtat cagcataaaa actgccgtct gaaaagtcaa aggtgaaaga
     4201 aatgtggcgc tataatttct aggactgata gtaaatcatt ctatcagtcc ttttgagcat
     4261 aaaaaaacaa aacatacaga agctccgtta gaattgaatc accacaacac aatcaaagga
     4321 gcgttctgta tgcctgatct tcatcttatc aaactattac taggaatcaa agatggaaac
     4381 attcaaatca ctcatgtcga gaagaaaaaa atacattcaa tcaacagtat cgttgtttct
     4441 gccacgctca ttaaaacgat tcagccctgt cctcagtgta aacaggtgaa tcaacatggg
     4501 ctgatcgtta agaacggaaa gaaaaaatcg ctggtccttc tcaataagtg tgcgaatcaa
     4561 cggacttatc ttgctttggc aaagcaacgg tacctctgtc gtggctgtca cacctatttt
     4621 acggcgaata cctacattgt tgaaaaaaac tgttttatcg ccaaacaagt acattataaa
     4681 atattggagg aattggctga aaaacaagca atgacgacga tttctaaaca ttgcggcgtt
     4741 tcttggtcaa ccgtttcgcg aacactcgct tctctgctcc ccatgacaaa cgtgaaaata
     4801 aattggttgc ctcgctgttc attggtagat gaatttcgtt cattgaaaaa tcaagtcggt
     4861 ccctattctt tttcttgtat ggatggcgat actgggaaat tactggatat tcttccttca
     4921 cgtaagaaga aagatctcat ttcttatttt atgcaatttg aacgacgtgc acgtttaaac
     4981 gtaaaaatac tcgtaacaga tatgaacgcc tcttatgctt tgctgataaa agagtgcttt
     5041 cctcatgcca aactcgttgt ggatcgcttt cacatcgtca agcacctcat tcgcagtttt
     5101 aaggatattt gactatgagt catgaaaagc ttcgaccgaa acgatccaat acaagccaaa
     5161 cactatcgac aagtgaaggc tttatctcga cttctgatca aacgacagga cacgcttgtt
     5221 tatgataaat ggacaaaatg gcgtaatttt ggttgggcct atttaaccga aagtgaagtc
     5281 gtagaacgtc tactttctac ttctgatgaa ctacgaatag cttatgctta ctatcaagaa
     5341 attcttcaag ctttttatga caaagaagcg gacatcttct ttcagcttgt aaaaacaatg
     5401 cccaagtcgg ttccaagaga actgcatcat ataaaaaaag cgtttataaa ctatgaatcc
     5461 ggcattcgtt tagccttaga gttgccttac tcaaatggca aaatagaaaa tctgcatacc
     5521 cacatcaaag cattgaaacg aatagcttac gggttaggat gagaaccaga attttcttaa
     5581 tcaatcatct gatcacttat taaacataaa aaaacatcta aagcagaaaa tcaattctac
     5641 cttagatgtt cttatatcac tctatcagtc ctatttgaca aagagccaga aatgtttgcc
     5701 gactatacac tagctttgtt gtgcaaaatg ctgagtatca atttccacag ggattgctag
     5761 aatatctttc agctgaatgt ataaggcatc gctaattttc tcttcccgat cgttcaaccg
     5821 atccactaaa atttctttgc cctccagcgt ttgaaaaaca taccgttgaa tgttgcctaa
     5881 aataaatgat tccttccata gaaccggaat cttaaccccc tgaggaaccg gttcaaattg
     5941 aatcagctcc ggtcttaaat aataaatgca gcgattgccc ttagcggaaa actcgtgggt
     6001 atactgattc agctcatacc attcgaaacg attatagttg ccgataaatt ccgcaacaaa
     6061 tcggctctga ggattctgat acaattcctt cgaggtggaa acctgagcca cccgcccagc
     6121 ttccatcaca aaaatccgat cactgattcg catcgcctct tcttggtcat gagtcacaaa
     6181 aatcatcgtc atccctagcg attgctggat agcgcgcaaa tcccgctgca gctgttttct
     6241 aattcgggca tccaaagcac ttaatggctc atccagcagc aggactttcg gttcagtgac
     6301 caatgagcga gccagtgcca cccgttgtcg ttgaccgcca gaaagaaact ggacattttt
     6361 atctgcctgc tccgtcatat ccaccaatga tagcatctca aaaacctttt cctgaatcac
     6421 ctcaggactc agcttcttta ccttcaaacc aaaggctaca ttttcaaaaa cagtcatagt
     6481 tggaaacaat gcatactgtt gaaaaacaaa gccgatattg cgatccttcg taggcttatc
     6541 ggcaatattc tcattgtcca gcacaatctc accgctcttt ggttgttcca atcccgtgat
     6601 acagcgcagc aacgtagact ttccgcaccc gctgggacct agtaaagtaa ccagctcacc
     6661 cttttcaatc tgaatttcat caatagctaa aatctgtttt tcatcaaaaa acagattgag
     6721 atcatgtatt tctaaaaaca ttttccgtca caactccttt aagggcttct ttttcgactg
     6781 cttctcttcc ctcgtctgct taagggacag ctgttgcttt tgtcgataag tcagcataaa
     6841 ggtagttaaa gcaattccca ccataaacag caaatacaaa accatcacag cgctagccaa
     6901 atggccgttt tcgttcatcc gccgcagcat atagatgcgt accgtctgaa atcgaccgcc
     6961 aatcaacagg ttcgtcagca tgtactcccc aaaaaccgaa gaaaaaatca tcagagaaac
     7021 tagggtcaca ccgatacgga tgtttggaaa cagcacctgg ataataatcg tagacatcgg
     7081 ggcgcccaag gtttctgctg catccactaa ttccttcaga ttaattaagc gcatctggtt
     7141 gttaagacta agatagacaa tcggcaacgc cgtaataaac aatgcaccta ccaacactac
     7201 aaacattggg ataccagttt cagcatacgt tcgaagcaaa gccgttacca aaatcactcc
     7261 tggtaaagca tacggcagca ggagcagctt ttccagccaa cgattcaaac gagggaaata
     7321 caaatgaatc caaataatgg tgggaatcat caacaacaga atcgtcacca agaccacacc
     7381 tgctaataaa agtgagcgac ccaccgcagc aataaattcc ctgtccgtca gcagctgttg
     7441 gaaccattgc aaggtaaaat cactaggtaa aagcgatttt gaccatttag tggagctggc
     7501 atacaaaatc gttccaataa tcggcagcaa gtaatacagg acaaagcaga acaaaatcgg
     7561 ttttattaac tttttcacgt ctgcaccacc ttgcctttct tttgaataat caacattgcc
     7621 gttaccagca ggacacctaa agcgactgcc aacgcactgc ctaaattagg cttggcgaac
     7681 acatctcccg acactaaagc cgcaattcga atcgttaaaa gatttacatt actgctggtc
     7741 agggcatacg ctgtttcata ggtccccatg ccattagcaa ataagatgac aaaggtgctg
     7801 gcaatcgatg gtcgcaaaaa aggcaaagcc accttgaacc aagcatacgc tgatgaagcg
     7861 cccagcaatt gtgcggactc cagccattcc tttttcactt ccttcatgct gggatacaaa
     7921 aacaagaccc ctaacggaat ttggaaatag gtatacgtga tggtcaatcc ccaccaagaa
     7981 tacaaattga agtccgctag aaatggcaaa atcattttca gcacacctac attccccaac
     8041 aaaataataa aagagaatgc cagcggaatc ccagcaaaat tagctgccaa attggaaaca
     8101 aaggtgattt tctcctgcaa tttagtcgat agattcatta agcataaaca taaaataaac
     8161 gtcccgatta acccaatcac actggagctg acagcaatca gtacgctgtt aacaaaggcc
     8221 tgtgtgtaat atttgttgct gaaaatatca ctgtaattgg ccaatgtcca ctggttagtt
     8281 aaatcactgt gcaagctgca cagaaccata aaaagcagcg gcagcagtaa aaatgcacag
     8341 aagataaagg aggcaggaac cagaaccaag cgttttttca tctctttcat cccctcatca
     8401 attcttgaat gtcctgcatt ttctctgacg gtgcaattcc taataaccag caaaacaatg
     8461 gagccgccag cagctgtggt acagtttgtt ccttcagctt ttggcgttta tttgaaatca
     8521 aataaaaagg aacttcacga tgtgcagcca agctgccgcc atgcagtccg tgcgcatcca
     8581 tcccgtgatc cgcagtcacc acgacttgat aacctttttg cagccattca ggaaggtaaa
     8641 tagccaataa tctgtccgcc tgattaaccg ctgctgcata ttcatgagaa tcggcggtaa
     8701 atttgtgacc gacatcatca atattcatcg aatgtacgac caaaaagtct ggctgatact
     8761 gattcactaa ataatgggca tccgcaaata aatgcgaatc agggtaagtg tcctcactat
     8821 aaaagatgcc atgctgaatc agtcgctcct cctgatgctg gatgcgatca accaaacgat
     8881 tatagggggc ttgattgtac aactcgctgt accagtggta agcggcggct cctgtttttc
     8941 cgccagcttc cttaactaat tcaaacagcg accactcttt ggatcgctga gcatggtaat
     9001 tactatagat gccattttca taggctggaa cgccggtcat cagtacttcg tataacggac
     9061 gtgaattgct gggcagctca ctttttacct tcaataagct tgcttgctgg tgctcaacta
     9121 aatgattcat gaacccaagc tgggcgacgg ctgtgtcgta gcgacagccg tctagtataa
     9181 ctacaatcag tttgtcaagt ctactttgca taggtcagta cctccgcctg ccaaagctct
     9241 ggaagctcta cagaggtctt gttccaagct tcattgtctt caactggcac cgccgcttca
     9301 taagcatcct ccggcagtaa tttttcagct acttctttag gcagctctac cttgcgaata
     9361 ggtcgcgcat agccctttgc caaattgatt tgcccttcat cagataaaat atattcccgt
     9421 gctagctttg ccgcattggg atgaggggca tttttattga ttaaggttgt atacccgcta
     9481 atgatggagc cgtctgttgg cacagttacc tcaaaacgct cggagtcaat ttggtcacga
     9541 taatttagtc cattgaagtc ccaaacaact gccacatcga tttcaccttt ttcaagattt
     9601 gctacgctgg catcaacggt ggacaaacgg cctttttctg ccattttttt gtagtaattc
     9661 agacctggct gaatatcctt ttcgtcacct ccgttggcaa tcgccgcagc caatacagca
     9721 aattgtgctt ggttggcaat cgtcacatcc cccacgctaa cttgatagtc accctcttgc
     9781 agctccttcc agctagtcgg tgcttgcttc acattctctt tatcggtgat aaaggaaatg
     9841 gttcccgtat agcttaacaa ccaatcccca tcatcatcct ttgcccaatc cggaatttcc
     9901 tcccaatagt tggtcttata aggtaaggtt aactctttgc tttcagccaa cggaccgaag
     9961 ctgataccga catcaccaat atcggcggtt ccattttttc cttctgattc aaacttagcc
    10021 aattcttctg cactggacat atccgtatcc acgtgactta agccgtattc tttttcaagg
    10081 tcgttccaag tgcctaccca atttgcccaa gtatccggca ttcccaagct ggcaatctct
    10141 ccttcttcct tggctttttc tataatcgta tctaaagagt cgtctgccgc agccgattgc
    10201 ttcgttgctt cacctgagcc gcagcctgtc agcaggatac ttactgttgc tgcacaaaat
    10261 aaaccgatag tcatgttacg aattttttca ttcattttca ttcattcttc tcccctttcg
    10321 ataattccat tctaacaaag ctttgtaatg agagaatcag tttcttgaaa aggtttcatg
    10381 aacaatgtaa aggttgtata tggtttgtaa atttcgacga attcccgtta accttctaaa
    10441 gcttttgcgg tatgatagta gaaaaaagga ggctccctat gcagtattat gatcagcatc
    10501 tccacacgta tttttccttt gattcagagg aaaaatttga aaactattta gcctatcagc
    10561 cggagttttt cgtttctacg gatcactttg atttaaacaa tccatctacc ggaaaagatg
    10621 acattccgga ttatccagcc tatataaaca aactggactc tttaaaagaa acctattcca
    10681 ccaaatttct gcgaggtatc gagattggcg ttgttccagg acaagaagag cggatcgtgg
    10741 attacctcgc tcagcatcct tatgatttaa agctggtaag tattcatcaa aatggccgct
    10801 ttgattatat ggacgatgtg gttttaacca aagatcccat tcaaactacc aaggaatatt
    10861 ttgatcagat gttgcaggtc ttgacggatt ttccagaagg agatattttg actcactttg
    10921 attatggatt acgccgcttc accttcacag cggaagaatt cgcggagcat tttgaggctc
    10981 agctgctaac gattttcaaa aaggttgttg aacgagaatt ggcgatggag ttaaatgcca
    11041 agagcttctt gaaatacggc aacgaagacc tttaccgtta tgccgttcct ttgtatcaaa
    11101 gtgtcggcgg aaagctcttt accttaggct cggatgctca tgttgccgaa gattatcagc
    11161 tgggctttaa aaaaatgagt cagctgttgg cagactgtca ggtagaaaag ttgctagtca
    11221 ttcaaggaaa agaacgccgc ctgacgccgc tgccgatttt gtgattccta tataatagaa
    11281 gaaagtggtg aaaaaaatga gcaacctgtg ggactacaat caagaggcac ccatccatta
    11341 tttaattgcg cgacattggg atgcgttgaa aattgaggcg gtttgccgat ccttgttagc
    11401 agccgttcca aaacaacaac tggaaaattt tttggtggct gacagcttgc agcgggaaaa
    11461 agttcaggct tattttgcag cctttaaaga tcagcctttg gaatatttgc atgcgcagtt
    11521 tcatcttttt tatcaagtag ccgccccaga tgactacaac gatttacgag gacagctgca
    11581 attgaccttt caagcggatg aaaccgccta taccgtttta ttgggaatgg ctcgccttgg
    11641 tgatcaggcg aaggtcgagt ggcggatttt cgatatttga tggcaggatc aaggcgtttc
    11701 agttgcaaac cttcctctct ttcaattaga aaaaccgcag caggaagaag tttgtgtgct
    11761 ggtcaccgat gctggtgaaa ttcgtctccg cttatttcct gaccaagctc ccctggctgt
    11821 agccaactgg cgctccctag ctgagcaagg cgtttatgac aacacacctt tcggctcgtg
    11881 tcattaagga atttgttatt caaggtggcg ctttggatgg ttctggtgat gaagctgttt
    11941 ccagctatga gggctttttc gctgatgaag tgcatcgcgg actttatcat tttaatggcg
    12001 ccttggcctt aggggaccac ggcccccaca ccaacggcaa ccagtttttt attgtgcaaa
    12061 acacaaaggc gcaggctgac ctgctaatgt aaagcaagca tacttggagc ttggaggact
    12121 accggagctg gatggtcgtt atactgtttt tggacaggta taccaagggt tagaaatcgt
    12181 agcagcgatc gcagctcagc cgacaaacag tgaagatgta ccgattgagc cagtgttcat
    12241 tcgacaaatt aacttcgaaa aaacatcgta aaaagacact cactgacttc agcaaaagct
    12301 ctattcttga gctggctgca gctagtgagt gtctttcatt tatccataag ataaagcttg
    12361 gattcaataa ataaataaat gttttggtta aagctgtgac cagcaaatga ggaaccgtgc
    12421 gagccagttg atctgccaat gcaaacattt ctttaggtga aggcgctggc gtgtctgcca
    12481 acggatactc cagtcccaat gcttggtaac cgtgcatacc atagcagtgg tacggtagta
    12541 attctaacgg tacgaaccga tcaagatctg ccacaaaaca agcaatcgtc agcagctcgg
    12601 tcaggttcat attgatacct ggaaccacag gaactcgaat tcgcacttcg cagttaggat
    12661 actcaattaa aaagcgacga atattcttca aaatttgttg gttatctaag ccggtccatt
    12721 gtttgtggag cttgctggta aacattttta catccacaaa caatgtatcc acatagggag
    12781 ctaccttttg gatttcatta taagcaccac aaaagctggt ttccagtacg gtattgattc
    12841 caatgtattt cgatttttgc aaaatttctt tagcaaaatc cgcctgaacc agtgcctcgc
    12901 ctccgctaat ggtcactccg ccatccgagt gaaagtaaaa aacagcatcc ttctcaatct
    12961 catccattac ctcttcagcg gtcatggtct ggccaaatcc tttttcaatc tgcttgcttt
    13021 gtgattcggg aatcgaacac caggcacagc gtaatgggca gccctttaaa taaacaacgg
    13081 tccgtaaacc agcaccatca tgtagagaac cctgttctat tcttaatact gttccttcca
    13141 tatagatttt tgaattacac actagtttaa actaaaacac acaaggaaaa acttaccatt
    13201 taatcataaa attagctcat ataaatatac taggatgtaa aatagactgg tcaaaatctg
    13261 gtcaaaaaag aggactaata caggtagtcc tcttttttgt tattttaata ttctaaatca
    13321 ttttttattg gtttattccc ttattcgatg tagactcaaa tcactatatc actagatatc
    13381 aaacggcgtc atacggaatc aaaaacatca aatttgacct gttaaatggt cattgaataa
    13441 cattcaaaat caataaaacg gagtcagaca cggagtcaaa aacgctgaga gtaaaaaata
    13501 tatacagcct tgtaggtaaa gtaccacagg gctgtatatt ttttatttca gattacctta
    13561 aatgattcca tttacttttt atctcatttt ttcctactag ttagtatcaa atactctcct
    13621 attttctcaa taaactattt ttggatacat ggtatctgaa acagctaaaa ccttgtcatt
    13681 acagcaaaaa atcacgttat ccagactgta gacaaagtcc attttatgga tgatgtctac
    13741 agtcttttct tttataatag atttatgaaa acgaatgcaa ggagtgatag ctatgatgtc
    13801 taaaaacaat acgaatggta gaaatcaatt tgcgatgctt acgattgatg atctagtt
//
LOCUS       DACCKU010000081        13179 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.084, whole genome shotgun sequence.
ACCESSION   DACCKU010000081 DACCKU010000000
VERSION     DACCKU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 13179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.084"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(199..1650)
                     /gene="cls"
                     /locus_tag="IVN13_002216"
     CDS             complement(199..1650)
                     /gene="cls"
                     /locus_tag="IVN13_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP7012678.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1991..4657
                     /locus_tag="IVN13_002217"
     CDS             1991..4657
                     /locus_tag="IVN13_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP7012679.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5021..5773
                     /locus_tag="IVN13_002218"
     CDS             5021..5773
                     /locus_tag="IVN13_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7012680.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5795..6949)
                     /locus_tag="IVN13_002219"
     CDS             complement(5795..6949)
                     /locus_tag="IVN13_002219"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP7012681.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7134..9575
                     /locus_tag="IVN13_002220"
     CDS             7134..9575
                     /locus_tag="IVN13_002220"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP7012682.1"
                     /translation="MKEVVMIDAARTPIGKYRGGLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9905..10609)
                     /locus_tag="IVN13_002221"
     CDS             complement(9905..10609)
                     /locus_tag="IVN13_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012683.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10796..11041)
                     /locus_tag="IVN13_002222"
     CDS             complement(10796..11041)
                     /locus_tag="IVN13_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012684.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11058..11909)
                     /locus_tag="IVN13_002223"
     CDS             complement(11058..11909)
                     /locus_tag="IVN13_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP7012685.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12075..13079
                     /locus_tag="IVN13_002224"
     CDS             12075..13079
                     /locus_tag="IVN13_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP7012686.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4205 a         2341 c         2754 g         3879 t
ORIGIN      
        1 gaggtcgtga gcctgccgtc cagctccgag tcacaaggaa caagttcaaa aaacagcttc
       61 tcatgtttgt tgaatattgt gacttgtgac cgaggagttg gcaggagaac accgtttgtt
      121 cggaagaagg gactgtgaca agatttttgt cacagtccct ttaatcttag tatatttttc
      181 tctacttgaa taaaaaaatt ataggattgg agaaaataat cgagaaattt gttgcttgaa
      241 taatagccag ttagacattt ccttgactgt ttcagttgtc atggatgaac aatgtgtctc
      301 atcttctttg aactgagtag caagttcttc taaaaactca ggatcgtaaa tgacagcact
      361 tgtttcaaaa ttcaatcggt agctacgaat atcttgattt gctgaaccta ccatgcagat
      421 ttcatcatcc attatcaatg tttttgcatg gaggaagccg ttctcataga caaggatttg
      481 tacattttcc ttgatcagct gccgagcata atattgtgtt gctcgataaa taaaaggatg
      541 atccggtttg ttgggaatca tgattttcac atctacacca gaggcagttg cgatttttaa
      601 agcagcaatg acactttcat caggaacaag gtaaggtgtc tgtatccaaa cacgtttttt
      661 agcagaagta atcaatttga taaatgaaag cttgatttgt tccctttcgt tattcggtcc
      721 gcttgaaaca agctggatgg atgtatcagc aagcttagat tcatctcgat ctgctttttt
      781 aaagaaataa ttcaaatgat agccgacctt tttttcttca ggtaccgaga cgttccagtc
      841 tgttaagaag cgaagctgga gcaaagaaga agcggcaccg aaaatccgta tatgcgtatc
      901 gcgccaatag ccgaattttt ttgttgtatt tacatattga ttggcaatat tgaaaccacc
      961 ggtataacta atctttccat cgatcacaac gatttttcgg tgatcatggt aattcaatcg
     1021 gaaacgaagt aatgcccttt gtgaggtaac aaatgtatgg acatgtccac catttttgat
     1081 tagacgattg aaatcttttg cttttgtgcc atgagagcca aatgcatcat ataatattcg
     1141 aacttccacg ccttctgcgg ctttttcttc taaaacatgt aagacttgct ggccgatatt
     1201 atctgtcaca aatgcgtaat attcgatatg gatcgaatgt ttggcttttt ttagatcttg
     1261 aagcagtgaa tccaatttct cttgtccgtc tgtgagaaga gtgacagaat tcattcttgt
     1321 cagcggcata cgatttaatg aagagaagaa gtcaacaaat tgctgtttgt ctttatcgcc
     1381 catgtcagga tcgtaatgtt caatggtatc tcctctaaaa gactgaaagt tttctaattc
     1441 tttcaagtca ctttgtcgga gatagaattt tttcttatcc gttaatccac gtccgaaaaa
     1501 taaatataat acaaagccca ccccaggaag ggcaaacaat actaagagcc atgcccaaat
     1561 ggctgctaca tctcggggtt tgatcaaaat agccaccaaa gcaataagtg catttaatag
     1621 ataaagggcg gttataatac tagataccac aaaaatgttc ctccttacat acaataacat
     1681 tagtatagca tgatagaaga aagaagccta ccaaggtgaa tatacttttc actcctttca
     1741 tgaaaatgta ggtaaatcgg gctaaagtag ttgctttttg taggttaata aggtaggata
     1801 ttagtacagt tgaatatctc cttaggggag taaccggcaa cgtttgttgc gataatatca
     1861 acagtaagtt aaccaaatgg ttttctggta ttgtatgaaa tggtgagacc taagacaaat
     1921 gcgtatttgt cttaggtctc ttttttttca gacaggacta gggccaagat caaaaaatgg
     1981 gggatgaaat ttggaaaaca aggaacaagc acaacaagcg gtctataaag aaacgatcga
     2041 ctcgttgatg cagcaaacgc agagctcaac cgatggattg agcaccacgc aagcgaagga
     2101 aaaattagaa agagatggct tgaaccagct aaaagaaaag ccgaagaagc caacttggaa
     2161 attgttctta gaaactttca aagatgcaat ggttatcgta ttgctgattg tcgcactgat
     2221 tcagatggtc atgggtgact acatcgaatt cttagttatt tttgcagtat tgatgttgaa
     2281 ttctataatc agtgttgtcc aaacaaaaaa agcagaaggt tctttagaag cgttgaaaaa
     2341 cctttcagcg cccgatgctc gagtgattcg tgatggtaag gaaaaaacga ttccggcaaa
     2401 cgaacttgtg gtaggagata tcgtgttgtt agacgcaggc gattatgtac ctgctgatgg
     2461 acgtttacta gatgcgggat cattaaaagt agatgaaggt atgctgacag gagagtcgac
     2521 ttctgcagaa aaagaagtaa cggatatcac aagtacggta ccaatcggag atcgtacaaa
     2581 tatggtattc agtggaacga tcgtaactta cggacgcggt aaattcttag tgacttcaac
     2641 tgggaataat acggagatcg gacaagttgc tggccttttg gaatcaacaa ctgagcaagt
     2701 cactccgtta caacgaggtc tggataaatt cagtaaaaaa ctaagtttgg ctattttggc
     2761 attgtcttta ttgattttag gtatccaact gtttcgtatt tatcttggag aaggtacagg
     2821 agatatgact gcagatatcg tcagcgcagt gatgtttgcc gtagccgtag ccgtagctgc
     2881 tattccagaa gcattacagt ctatcgtaac aatcgtgtta tctttgggaa cgaataaaat
     2941 ggcaaaacgt catgccatta ttcggaagct tccagcagtt gaaacacttg gttcgacaag
     3001 tatcatctgt acagataaga ctggtacatt gacacaaaac aaaatgacag ttgttgacta
     3061 ctttttaggt aatggcgata ctggggattt cacaaatgac ccttcaaaat ggacagctga
     3121 cgaacgtcgc ttgatcgaaa tcagtgtttt agcgaatgat gcatcgatca gtgaagacgg
     3181 tactaaattg ggcgacccta cggaagtagc gtttatcgac tttagtgaaa aattaaacca
     3241 gccttatcag gaaatccgaa acaactaccc aagacaagca gaacttcctt ttgactcaga
     3301 tcgaaaatta atgtctactg gacacacaat tgataacaaa aattacttgt ttgttaaagg
     3361 cggaccagat atcgtcttcc aacgcagtaa aaaagtattg ctaaatggtg aagtcgttcc
     3421 gatgacggat gaattgatga aaaaattcca agagcaaaat gaagcttttt caacacgcgc
     3481 attacgtgta ctagcttttg cttaccgtcc acttgataat tttgatttat catttgaaga
     3541 tgaagacgat ttgatccttg ttggtttgct tgctatgatc gatcctccac gtgctgaagt
     3601 gactcaggcg gtaacagaag caagaagtgc aggaatcaaa acaatcatga ttactggtga
     3661 ccacaaaaca acagctgttg ctatcgcaaa acaaatcggg atttttgaag aaggaaacct
     3721 ggctttgacc gggacggaac tagatgaatt gacagaagat caactaaacg aagttttaga
     3781 aaaagtcact gtttatgcac gtgtttcacc tgagaataaa attcgtatcg ttcgtgcttg
     3841 gcaaaataaa ggtcatatct cagctatgac aggtgatggt gtaaatgacg caccagcatt
     3901 gaaacaagca gacatcggaa ttgctatggg aacgggtaca gatgtggcaa aagatgcagc
     3961 agcgatggta ttgacggatg ataactttgc ttctattgtc agcgcagtag aagtaggacg
     4021 taatgtgttt gataatatca agaaagctat ttcttatcta tttgcaggaa atttaggtgc
     4081 gatcattgcg atcatctttg ccttagttgc tgactggtcc aatccattta cagcattaca
     4141 actgttgttc atcaatttag tcaatgattc actgccagca attgcattag gtacagaaaa
     4201 agctgaacct gctacgatga accgcccgcc aagagatcca aattcaggaa tctttacgga
     4261 caaaacattg atttctgtaa cgtatcgtgg tgtgctgatt gggattgctg ttatctttgc
     4321 tcaatggctt ggcatgcagc attcacctga attcggtgta gccatgtcat tttcaacatt
     4381 gatttggtgt cggacattgc aaacattccc agcacgttca aacacacaaa cagctatcca
     4441 agcagggttc ttctctaaca gaagtgtctt actagctgta gttgtatgta gcgcgcttta
     4501 ctgcttaaca atgatcccag gactgaaaga agtcttctct atcccatcag catttggatt
     4561 gacagaagtc tttacatgta tgggaatcgc acttgtgaca attgttttga tggaaatcac
     4621 taaattgatc attgtacgag tacaaggaaa taaataaaac gaatcaacga ggttgtgagc
     4681 ctgccgttta attctgagtc acaaggtgca atttcaacaa acagcttctc atgtttgttg
     4741 aatattgtga cttgtgaccg aagagttggc aggagaacac cgtttaagga agaagttgta
     4801 aaatcagcat tttgagctaa tactgagggt catctgattg gacactgttt attcggaaag
     4861 agggactttg acacgacttt ggtcacagtc ccttttttta tttatgatag acggaaagcc
     4921 aatcatttta ttattcatca aaagagatag aatagataaa agaggagaat gatttattcc
     4981 gaaaagtgaa aacatataac aaaaaattag aggaaatcaa atgaatgaaa aagaatggga
     5041 tgaatttgct gaagaatatt atgaaatcca gcaagaatcg caagtaacta ttgctgaaga
     5101 tgtaaaaaat tatctgaaaa cggagaaagt cctgcctgct gaaacagcag cagacgttgc
     5161 tggaggatct ggcagatttc tttccttagc aaaagaaatt aatcgctatg atttgatcga
     5221 tttttcttca ggaatgctag aaaatgcaaa aaaagaagca gaaaaaaatg agttgccaaa
     5281 tgtttccttt atcaaacagt cgttagaaga atttatccaa tcaaatgaga catatgaaat
     5341 ggtttttaca gcagcaaatc cagcattggt acatcttatt caattagaaa aattgatgaa
     5401 aaagtcaagc aaatggtgcg tgataatcag agtagtggaa tcaatcgatg atgtttttta
     5461 tcctatcgaa aagcaactag gaatgataga agaagatcct aatacatcgc cagttgtaat
     5521 ggatcaattt gaaacatact taattgatga aggattcaaa gtgaagaaaa aagattttac
     5581 ttattttaca caggaatcaa ttactgaaga attgatgaag ctatactatg acgcgtacgc
     5641 aaaagatcct gttgtgacca ggtacattcg caaactattc agtgaaaaac gagaaatcct
     5701 tagtacgact aagctgacct atagactgct cctttggtcc gcagataaaa aaagaacctt
     5761 tcacgactcg tgaagggttc tttttatgat tttattatat tttgtagtaa cgaatatcat
     5821 tttcgatctt tgtaatcgaa aaaggtacat ctgaagtata ttctgcacat tctccgtgtt
     5881 caggaagcgt ctcactgaaa atcgtttcat attcgtcaac agtgatccgt gttcgagaat
     5941 ctagcatctc ttgatgagat tgtgcgaaga gatattcttg atagttttct tccagataac
     6001 ctgtaaaaaa ttcacttact gcaccagaac cgtagctgaa tagaccgatg cgatctcctg
     6061 gctgtagtga tttcgaattt tccagtagag aagttagccc caggtataat gaaccagtgt
     6121 aaagattacc gattcgtcgg ctgtaacgga tgctttcttc atagcgagcc ataagacgtt
     6181 cctgattttc ttcgtctgtt tggtctaata cgctttgcaa tgcctttttt cccatcttag
     6241 tatacggaat atggaaagca atcgcctgat aatcttcgag tcctcgaccc gacaattctt
     6301 tatgtcgatt ccaaactttt tggaatgatt cgatatacgt agaattagat aaaggaccat
     6361 caacaacagg aaattcgcta taatctggac gccagaaatc atagatatct tctgtcagaa
     6421 atacgctgtc gtcttcaatc gataaaatac gcgggttttg agtgatcatc atcgcaacag
     6481 caccgacacc ttgcgtcact tcaccaccgc ttgccaagcc gtaacgagca atatcacttg
     6541 ctatgactag tacttttcgt tctggatgat ttttgacata ttctttcgcc atgtgcaaag
     6601 ctgcggtgcc accgtagcaa gcctctttta cttcaaaaga acgagcaaaa gattggattt
     6661 tcagtaaatg gtggataatt accgcactcg cttttgaatg atcaatcccg gattctgttc
     6721 cgacgatgac catgtcgatt agctcacggt ctttttctgt tacgattttg ctggcagcat
     6781 ttgctcctag tgtaatgatg tcttcatttg cacgattgac tgccatttgg tcttgaccaa
     6841 tccctatatg gtattttgca ggatcatccc cacggctttc tgccaactct gtcatatcta
     6901 aatataaatt aggaataaaa aaggaaagac gatcaatccc tattttcatt tcaacacctc
     6961 tttacttatg ataaaaaact tactttacta aggtatcata aaaagattaa aagaaatata
     7021 aaaaagagaa tatttaagga ttgttaaaat ttaatcttag aaaaaaataa ctggaacaag
     7081 agctatttgt ttttacagca aagaatggta tatttttgta ggaggtggct tacttgaaag
     7141 aagtcgttat gatagatgct gcaagaacgc cgataggaaa gtatcgcgga ggtttaagtc
     7201 catttactgc agttgaatta ggcacacttg tgactaaagg attattagat aaaacaaaac
     7261 tgaaaaaaga taaaatcgat caagtaatat ttggaaatgt cctgcaagct ggtaatggtc
     7321 aaaatgttgc cagacagatt gctttgaaca gcggtctccc tgtagatgtc ccagcaatga
     7381 cgatcaatga agtttgcgga tctggtatga aagcagtgat cttagcacgc cagctgatcc
     7441 aattaggtga agcagaactt gtgatagccg gcggaacaga gagtatgtct caagcaccta
     7501 tgctgaaaca gtatcagtca gaaacaaatg aatatggtga accaatttcc agtatggtca
     7561 acgacggatt gactgacgca ttttcaaatg cacatatggg attaaccgca gagaatgttg
     7621 caacacaatt ttctgtgagc agagaagagc aggatcgcta tgccttgtcg tcccagttga
     7681 aagcagcaca tgctgtcgaa gccggtgtat tttctgagga gatcatccca gtcaagattt
     7741 ctgatgaaga cgtgttatct gaggatgaag cagttcgtgg aaatagtaca ttggaaaaac
     7801 tgggcacgtt acgtacagta ttctcagaag aaggaactgt aacagcagga aatgcttccc
     7861 cgttgaatga cggtgcctct gtggtgatcc ttgcatccaa agaatacgca gaaaataata
     7921 atctgcctta tttagcaacc atcaaagaag tagcggaggt cggtattgac ccaagtatca
     7981 tgggaatcgc tccaatcaaa gccattcaaa aattgacaga tagatcagga atgaatttat
     8041 caacaatcga tctttttgag atcaatgaag catttgcagc ttcttctatc gtagttagcc
     8101 aagaactgca gttagatgaa gaaaaggtca atatatatgg aggagcaatc gcattaggcc
     8161 atccaattgg tgcaagcggt gcacgtatct tgactacatt agcttacgga ttgcttcgtg
     8221 aacagaagag atacggtatt gcttcattat gtattggtgg aggtttaggc ttggcagtct
     8281 tgttagaagc gaatatggag caaacacata aggatgtgca aaaaaaaaag ttttatcagc
     8341 ttacgccatc tgaacggcgc agtcaattaa tagaaaaaaa tgtattgaca caagaaactg
     8401 cgttgatttt tcaggaacaa acactttctg aagagctctc cgatcacatg atcgaaaacc
     8461 aagtgagtga agtggaaatc ccaatgggga tcgcgcagaa ttttcaaatc aatggcaaga
     8521 aaaaatggat tccgatggca acagaggaac catctgtcat tgcagctgca agtaatggtg
     8581 caaaaatctg tggaaatatc tgcgcagaaa cacctcagcg gctgatgcga gggcaaatcg
     8641 ttctatcagg aaaatcggag tatcaagcag tgatcaatgc agttaatcat cgaaaagaag
     8701 agctgattct ttgtgcaaat gaaagttatc cttcaatcgt gaaacgcggg ggcggtgttc
     8761 aagatatctc tactcgggaa ttcatgggga gtttccatgc atacttatct attgattttt
     8821 tagtggatgt aaaagatgca atgggcgcta acatgatcaa ctcgattctt gagagtgtgg
     8881 caaacaagtt acgggagtgg ttccctgaag aagagattct attcagtatt ttaagtaatt
     8941 ttgctacaga atcattggct tctgcctgtt gtgaaatccc attcgaacgc ttagggagaa
     9001 acaaagaaat cggtgaacaa attgccaaaa aaatccaaca ggcaggggaa tatgcgaagc
     9061 ttgaccctta ccgtgcagca acgcacaaca aagggatcat gaatggtata gaagctgtag
     9121 tggcagctac aggaaatgat acacgtgcgg ttagcgcttc gatccacgcc tacgcggcaa
     9181 gaaatggatt ataccaagga ttaacagact ggcagatcaa aggggataaa ctagttggaa
     9241 aactaacggt gcctttggct gttgccacag taggcggagc ttccaatatc ctgccaaaag
     9301 caaaagcatc tttagctatg ttagatattg attcagccaa ggaactagca caagtaattg
     9361 ctgcagtagg gttagctcaa aacctggctg ctttgcgtgc attagttaca gaaggaatcc
     9421 aaaaaggcca catgggactt caagctcgtt cattagcgat ctcaattggg gctattggtg
     9481 aggaaatcga gcaagttgcg aaaaaattac gtgaagcaga aaagatgaac cagcagacag
     9541 ctatacaaat attggaaaaa atccgggaaa aataaaaaag caatatcttt agaagaataa
     9601 agcaagattt acagttattt tatttactgc agatcttgct tttttctcta tcagaaaata
     9661 tgtttattat tagaattttt tatagttaag gttaggcatc ttaagaaatc aggcaactcc
     9721 aagatatctt caggtagaag agaagtcgcc taacattgta ggatcatagg tgatttgttg
     9781 gacaggtcaa gctgatattt gagcctgatg ttagaaggaa tttcttttta cttgctgtag
     9841 aaattattat atgactaaaa aggctgggat tttaaatccc agccttttat gacaaataga
     9901 atttttatcg atcagtaatc gctgtcgctg caaattgttc gatcaaagga atcaaagtca
     9961 atttgacttt gtatttccgt tcgagtgctt tttcgataac gaactcattt tcagtagtca
    10021 tagaaatagt tgctaattta cgaggttcat cagcattttt ttcattaata actacttcat
    10081 gttcattttg ttctgtgtaa aatttaggat actcagcaac ggcttttgaa aaatcagcaa
    10141 aacttaaagc ggcattcgta tcagttgagc gtgtattttc agttgtgtcc gcttttggtt
    10201 tttccgtttt ttcttctggc acactgactg tttcttctgt aactggtgtc gcaacagctt
    10261 gcgcagttgg ttcatcgttt ttcaatcgtt gatcattcca gtaagacacg atttcaataa
    10321 gaagattgtc acggaatcgt tcgaaaatcc cgttttctcc tgaacgaatt ctctttaata
    10381 cagtattgaa gttgtcatcg attggcacgt tgatccgtgt atcttcattt gttttcccag
    10441 ctaatccgat cgttaagatt tgacggtttt tgggatcaag catgacttgt aacggcaaac
    10501 cataacgtcg caagctctct tcttctagag aacggaataa ttgtcctctt acaccatcca
    10561 atgaaggacg gaattgattt aataattttt gtttctgttt tttcttcata tgttctctcc
    10621 ttcgctcata ccctttcgat tataagaaaa aaacagccaa atgaaaagga gtaatgcaaa
    10681 aaaacttgcg tatacgcaaa ttacaacaga aaaatttgcg tattttcccg ttttttgaaa
    10741 gaaaaataaa aaatggaacg gattatgtat atatacataa tccgttccat tctttctatt
    10801 ttttcggtat caaggcaaaa acaggtagtt caggtgaata aatgacttca tgttcgtgcc
    10861 agggtttgac cattagcgaa tgatcgaacc gttttttcag ttcttcgatg atttgatcat
    10921 gtgtccagtt tcttgcagga aaatgttgga ttttctctgc atcgatcaaa aagacaaaat
    10981 cggaatgaaa agtctgggta acttcttccg agagagaatt caagcgttcg attggatcca
    11041 taaaatcacc tttcgactta aaattgggta gtatctgggt cttttgcttt gccgactaca
    11101 ccatctaact gatcgatttg tttcatttct tcttctgtta attcaaaatc aaataattca
    11161 gtattttctt tgattctgct tggtgtcact gacttaggaa gaggaaggaa ttcgtgttgc
    11221 agcgaccaac gcaaagcaac ttgtgcaatc gtcttgtcat gtgcttcagc gatttgtttc
    11281 atttcaggca catcgaagat tttaccagtg cctaatggac tatatgcttc gaggatcata
    11341 tcatgttttt ttgcatactc aactacagtt gtctgtaatt cgcctggtgc aaggaaaact
    11401 tgattgacca tcggcataat ttttgctgtt tttgctagtg tatctagatg gtggggtaag
    11461 aaattgctca caccgattgc tttgatttta cctgcttcat acagttcttc aaatgctcgc
    11521 catgtatctg catttgcttg ttcccaatta tcacgaaaag caacagggtt tggccagtgg
    11581 atcaaaaaca gatctaaata atctgtttgg agcttcttca atgattcttc aaaagaagac
    11641 atcacaagtt catagctgtg gtttgcattc cataattttg ttgtcaagaa tatctcttct
    11701 ctaggaattc cactttcctt aatcgcttgg ccgacgcttt cttcattctt gtatccttgg
    11761 gcagtatcga tatggcgata acctgcagcc agcgcttctt taacagagga aacagcgaca
    11821 tccccatcag gtgtttgcca tgtgccgaat cctactaccg ggatttcgta accatttgat
    11881 agacgataag tgcttgttaa tgaattcata ctttcagctc cttttttatt cgttcaacta
    11941 taagtgtagc gcatttttcg aaaaaaagaa tccattttgt ttctactctt gaaatttttg
    12001 tttaaaagaa aatgattttg attctggtat ttttgatttt ttttccgtaa actaagacag
    12061 aagggaatga gaaaatggat atctatttaa aaaaagcaat tctgcatatt attgacagag
    12121 aaactggttc tccagtcttt tcacaaaaag agctggatct gacaaaagaa tatattcgtg
    12181 attttcttca aaagaagatc caaaaaatct cttctgctca aacaaaaact ggacaactag
    12241 cagaagaaag catgatcgtt gaatcactta aaatcgctca agaaaatttt gtggaagcaa
    12301 gtgaagcaat cgttcagcgg tggttcgata tctaccaaga aagtgaagaa gcacctagtg
    12361 cagatgtatt tgttgtcttg tatgaattag atacagtgat gtacttatct ttattgaaag
    12421 tcaattatcg tgaggcttac actcactttg ttgaagcaga cgaagcaggg atcgacaacc
    12481 gcttgatttt gaatcgagca attctagggg cgaaaacaca aaaagcagat gaagcattcg
    12541 ctgtgaattt gactgattgg acctacgaat taattgaaaa aaaatatgag ttttctggca
    12601 aaaaagagct ttatttttct ggaagagtga tcgaaagtgt tcctgcacct tctttagaag
    12661 aaaatgtaaa agtgatccaa aaagtagcca agcatcttgg aaaaaaattt gagacagaag
    12721 aatttgatat catggctgat ttgaaagaag ctgtctatga tacgatagaa gaaaaaggtc
    12781 ggttggatca tgagatgatt gcggaaaaag tcttcaagga aaacatcacg gcaaaaatgg
    12841 cttttaaaga agaagtacaa gagcaaggat ttgtcccaga agcacctcct gtaaaagaag
    12901 tacaagaaat ttctgagaaa aaatttggaa aacaaaaatt caagctatca aacgggatcg
    12961 aactgatcgt accagtagat gtatatcgca acccagattt gattgaattt gtgaataatc
    13021 cggatggtac gatatccgtg atgatcaaaa atgtggatga agtattgaat cgtttataaa
    13081 aatgactaga atattaggtg ttcaagtacc agtgatcagt taaatgaatt gtgtcaagag
    13141 aaggatcgga aaagacgaat ataacaaaaa aagagcgtg
//
LOCUS       DACCKU010000082        12999 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.085, whole genome shotgun sequence.
ACCESSION   DACCKU010000082 DACCKU010000000
VERSION     DACCKU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12999
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.085"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            224..658
                     /locus_tag="IVN13_002225"
     CDS             224..658
                     /locus_tag="IVN13_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7012687.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            759..1850
                     /locus_tag="IVN13_002226"
     CDS             759..1850
                     /locus_tag="IVN13_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP7012688.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            1880..2557
                     /locus_tag="IVN13_002227"
     CDS             1880..2557
                     /locus_tag="IVN13_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP7012689.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            2889..4184
                     /locus_tag="IVN13_002228"
     CDS             2889..4184
                     /locus_tag="IVN13_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7012690.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            4174..5634
                     /locus_tag="IVN13_002229"
     CDS             4174..5634
                     /locus_tag="IVN13_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7012691.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            5770..7266
                     /gene="glpK"
                     /locus_tag="IVN13_002230"
     CDS             5770..7266
                     /gene="glpK"
                     /locus_tag="IVN13_002230"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP7012692.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            7280..9103
                     /gene="glpO"
                     /locus_tag="IVN13_002231"
     CDS             7280..9103
                     /gene="glpO"
                     /locus_tag="IVN13_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP7012693.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            9104..9820
                     /locus_tag="IVN13_002232"
     CDS             9104..9820
                     /locus_tag="IVN13_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP7012694.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            10126..12055
                     /locus_tag="IVN13_002233"
                     /pseudo
     CDS             10126..12055
                     /locus_tag="IVN13_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            12181..12735
                     /locus_tag="IVN13_002234"
     CDS             12181..12735
                     /locus_tag="IVN13_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP7012695.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
BASE COUNT         4347 a         2165 c         2934 g         3553 t
ORIGIN      
        1 gccattttca caaaattttt cttcatactc aaaactaaac gactttttca caacctctga
       61 ttcacggtgt tccagaagga gttttgtcga aaacaagccg aaattctttg aagatggcta
      121 cttctttttt tcaaatctag acagaggagg cggacattat tccgtgtttt ttgacaaaaa
      181 ataaaaatca tactatgata tgtacgccaa tggaggggat gaaatggaaa agccaattga
      241 aaaagcgtta gatgaatgtc ttttctttag cgtgaaaaag ctagatcgca tgttgaataa
      301 attagctgat gaagcgttta gaaggacagg tctggcacca acttatggtt ttattttatt
      361 gatattaaaa gaaaaagatg gaataccaca aaaagatatt gcacaaatgc tctattccgc
      421 cccatcaacg attgctcgtt ttgttgaaaa attagaatat aaaggatacg tcaaaacagt
      481 tagcgaaggt cgcttgtcat tagtttatct tacagaacaa ggacgccaat ttgctaagga
      541 agtcgatggt agctgggaag aacttcatca gtcatacaaa gcagttttgg gaaatgaagc
      601 aagtaaagaa ttagcccaga caatgaatga tgcaacagat aagttacaaa aaaaataaaa
      661 ttttttaagc aaattagttg catgtacaac taatcgagtg gtttaatgat agatgattct
      721 cgataggtta atcagagcga attatgggag gaatgacaat gtcacaattt gatcaatcaa
      781 ttactttacc ttcaggagtt caattgaaga accgattgat gctgtcgcct atgacgacac
      841 aactcagctt tttcaatgga gtgatcaccg aagatgagat cacttattat aaacaacgtt
      901 ctaaaggtct tggtgctgtt attactggtg ctgcaaatgt ccaagatatc ggaaaaggct
      961 ggccaggtga actcagcatc gctcatgatg aaatgattcc tcgattaagt gagctagcga
     1021 aagcaatcca aggagaaggc gcaaaagcga ttattcagat tttccacggt ggacgaatga
     1081 caagtcgagc aactttatct ggagaacagc ctgtatctgc aagtgcagtt gcagcaaaac
     1141 gtcctgatgc ggaaacacca cgagcaatga gtgaagaaga aatcgttgaa acaatccaag
     1201 catttggcaa agccacacgt agagcgattg aatcaggatt tgatggaatc gagttgcatg
     1261 gagcaaatac gtatttgatc caacaatttt tctctccgca ttcgaataga cgagaggatc
     1321 aatggggcgg tagcttggaa aatcgttatc gttttattga agaattatta acaacggttt
     1381 tctcagcggt tgaacaatat gctaaaaaac cattcatcgt agggtatcgt ttctcaccag
     1441 aagaatatga aacaccaggt atccgttttg aagacacgat ctggcttctt gaacgattac
     1501 gtgagtcaaa actagattat ctgcatgttt cattaaatat gtatgatcga atagctcgtt
     1561 ctgataaata caacgataaa tcgattcttg aagtggtcca taacacgatt caaggaaaga
     1621 tccctttagt tggtgtcggt gggatccgca atcgcgaaga tgtagagcag gtattgaaat
     1681 atgcagaact tgcagcaatt ggacagcaaa tgatcgtcga tcctgactgg gcagagaaat
     1741 tattggaaaa ccgtgaggat gaatttgtaa cgaaaccttt tgaagaagca tataaagagc
     1801 tttatttgcc aagcccatta tataatttct tgaaagcacg ttatcaataa cgcaattttt
     1861 atagaaagag gatgggaaaa tgaaagtatt gatcgtatta acaaatgtag aaaagtacga
     1921 aaagatcgag cgtcctacag gtttgtggtt aagtgaactg actcattttt atgatgtatt
     1981 agtaaaaaat gggatcgatg cagattttgt cagtcctaaa ggcggatatg tacctttaga
     2041 accgcaaagt gttttgaata tggatgaaat cgattgggcc tattatgaag atgaagattt
     2101 cagaaatcgg gccttgggaa ataccttacg tcctgatgat gtggatgcaa atgattatgc
     2161 agcggtttat tatgctggtg gccacggaac aatgtgggat tttccagaag caatagatct
     2221 tgggaaagtc gcttcacaaa tctatcgaaa tgaagggctg gtctcagctg tttgtcacgg
     2281 tgttgtagga ttgttgccag ttacagatga gtctggaaaa tctattttag ctggacgtac
     2341 agtgactggg ttcagtaatg aagaagagga agcaaatggt acaacgaatg aagtaccatt
     2401 cttagcagaa gatgctttga aagaaaaagg agcaaactat cagtctggag cagctttttc
     2461 agaagtggtt caaattgatg ggcggcttct tacagggcaa aatcctcaat ctgctcatca
     2521 attaggcgaa gcagttgttg aagcattaaa gaattaaaaa taaatgatgt gtctattcta
     2581 actattacat tacacacgct aaaactcgtc ttttcgttct tttctgtttc ctatttggac
     2641 agaggtacgt aaaaggcgag tttttttctt ttataagagt acattttttg aaacatctct
     2701 tcagaaatga tactcttcaa ttaggtccac tagtacatta ttgagaagca gacctagaac
     2761 acggtatgat ggagagggtt gattctgctg catcgctatg tatcataagg ttgaaagcga
     2821 gttatgaata ctgtgttttc tgattgagaa gttagcagta gaacacctta taagaaagag
     2881 gaatgcagat gaacattgta attgtcggcg ggtcgtttgc agggattcat gcggctattt
     2941 atcttcgcca attgatgcca acaagtgaaa tatatttgat tgaaaagcaa tcgaagttag
     3001 gatggattcc cagcgggttc aatttgatat taaaaggaaa ggttaccagt gaaaaacaac
     3061 tttcttggat cacgaaagaa gaactgactg accgctatcg aatccatgtt tacacagaaa
     3121 agacagtaat aggattgaaa gaaaaaaaag tcgttttagc aggaggaaaa aaattggatt
     3181 ttgatcgttt gattctagcg actggttcaa atcaaaagtt tcgaaatatt tctgcagaat
     3241 caacgcttat tcacccagtt aaaaagatac aaaacgtaac ggctcttcag caaaagatca
     3301 tgaatgcaaa gaaaattgca attattggtg caggacaagc aggaatcgaa atagctgaag
     3361 ggttggcatc aagaaaaaaa caaatccatc tttacgaaag ccgaaagtca attctttttc
     3421 gttatctaga tcctgaaatg acagaaccgt tagtaaagga aatgaaaaat caagggcttt
     3481 cacttttttt agaagaacaa gttgtgtctt tgaccgaaaa tgaaaccgct attgtagaga
     3541 cggaaaaaag aaaggagaac tatgatcttg ttttgctggc taatcattct cgtcccgaca
     3601 atcagatgtg ggaagaacaa ttaacattga atgatgatgg aacgatctgg gtagatgact
     3661 acttgcaaac gtctcaaaaa gacgtttatg cgattggcga tgcgatccaa gtaacttttc
     3721 gtccaacaaa tgaaaaaatg tatgtttctc tagtaaataa cgctgtccgt acagcacgaa
     3781 atgtcagcaa gacaattagt ggctcacaga caaaggatca gggaacctat cgtccagtcg
     3841 gcaatcagtg gtttggctat ttcttaggaa gcgtaggttt gacagaagaa gagagcattt
     3901 tttatcccca aaaaattgaa acaggatacg tagactacaa gcttacagca ataaatcaaa
     3961 gcaaagttaa actcaaattt ctatttgatt ctgaaaaaaa gattcttgga gcacagctcc
     4021 aaagcaaaaa agagatgttc caccttcttg accaactgac aattgcagta gaagaaggat
     4081 ggacgtttga acagttagaa gaacatgagc tatttttcca gccagaatac cgtgtgccag
     4141 atatggtgtg gtcaaaggtg gcgaatactg cctatgaaga ttgaagcatt attagaaaga
     4201 aaagagcagt tacagatcct gattttgcga aaccttgttc tgcaaggggg aacagcctct
     4261 atcaatggtt tgagagagca tgtccaactg tcaaaagcct cgtttgatca atatttggaa
     4321 gatattgaat tgattggccg aatgatggaa aaaaaagtag aagtacagcg aaatgagtat
     4381 caggcgctgc ttgtgttgga cgaagatgta agtttggaaa aaattctatt gtttttattg
     4441 caggaaagct tgaagtttaa gatgctcgtc tatttattag aacatcagca agtatccatc
     4501 gttcgattag cgacagcgtt taatatcagt gagtcttctg tttttcgaaa gatcaaagaa
     4561 ttgaaccagc tcttagagga atttggatta caaatcaaaa acggtcaatt atacggagaa
     4621 gagctacaga tacgatattt ttattatgaa ctattccaat atatcccgga agatcagcga
     4681 ccgctttttt tgcaaaatac accagaaaaa agaccgttca ttctaggatt ggaccgtgta
     4741 ttagaaacaa cgtttacggc atcagcagaa gcacagattg cttgctggct cgggattacg
     4801 aaaaaaagac tattgaatga aaaaagtacg tacgcaactt tgaaagaaaa gaaattactc
     4861 tatcagtcgg accggcttta tcaagcaatc gatccaatca tcgtgatgca tcttagtcga
     4921 acagcagcag aactgaatgt atacgaaaca atgatgtttt acagcttttt tgtttctttt
     4981 tcgatagtag acgaagaaat attttatcag tatgatttga cacggagcaa aaagttacca
     5041 accgctgttt tagatactta cattcgggaa acgatgttat ggcattatcg tcctcgtcgt
     5101 ttgaaaatca aagaagaaaa ggcagtaggc taccaaattt cccagatcaa caatgaatgg
     5161 tttttctttg ttggaaagat cgaagtctat gagcgtgatc ggcttttgga acagcagcag
     5221 aaaatgctcg ggcattcctt aacacagtta ctggcgaaat tacaggaaac agcggtgcag
     5281 caattgccga ggaaaagagc tgaagatagt gagcttagtt acctgatgat ccagtatgcc
     5341 aatgttcttt tgatgatcga tttttatatt gcaaaaccag tagtgattgg aatcgattta
     5401 gaaagtctgc cgatttatcg tattgctttt caacaatatc tgatcagaga actgagggga
     5461 atcggcggta ttgagattgg aagttatgaa gaaggtaaag aatatgattt ggtgatcaca
     5521 ttttgccaga gaaacaaaaa acagtgtgaa tattatctgt cagaatttgc gtcaccttac
     5581 gacattattc gattgaaaag aagaatagag acgctgaaaa aagaaaagaa ttgattaaaa
     5641 aatagcttca taataaaaaa ctgtcaaaaa aagaaaaaaa gaataaaatt ttgacagttt
     5701 ctttttttaa aacagtagta tgataagagt agttaaagaa agcgtttgca tataatggag
     5761 ggaatcacaa tgacagaatc aacgtatatc atggcgattg accaaggtac aactagctca
     5821 cgagcaatta tctttgacaa gaaaggaaga cacatcggca gttctcaaaa agagttcacg
     5881 caatatttcc cgcaagaaag ttgggtggaa catgatgcaa atgaaatctg gaattctgta
     5941 cagtcagtta ttgccggagc gttcatcgaa tcagggatca aaccaaatca gattgctggg
     6001 attggcataa cgaaccagcg tgaaacaaca gtaatatggg aaaaagatac aggacgtcca
     6061 atctatcatg cagttgtttg gcaatccaga cagtcagctg gaattgcaga taacttaaaa
     6121 aatgaaggct atcaagaatt tttccacgaa aaaacagggt tagtgatcga tgcctatttc
     6181 tcagcaacga agatacgctg gattcttgat catgtagaag gcgcgcaaga acgagctgaa
     6241 aaaggcgaat tgatgtttgg aacgatcgat tcttggttag tatggaaact gaccgatgga
     6301 caagcacatg ttactgatta ctcgaatgcc agtcggacga tgttgttcaa tatccatcag
     6361 ctagattggg atcaggagat tttagattta ctaaatattc ctcgagtgat gttaccaaaa
     6421 gtcacttcta actcagaagt ttatggttat acgcaaggtt atcattttta tggaagcgaa
     6481 gcaccaattt cagggatggc aggagaccag caagccgcat tattcggaca aatggcattc
     6541 gaaccaggca tggtcaagaa tacttatggt acaggttcat ttattgtcat gaataccggt
     6601 gaaaaaccgc aattatccaa aaataattta ttgacgacaa ttggttacgg cataaatgga
     6661 aaggtttact atgctttaga aggaagtatt tttgtggctg gttcttcgat tcaatggctg
     6721 agagatggac ttcaaatgtt gcaaaaagct agcgattcag aagacgcagc gaaacgctct
     6781 acaagcgaag atgaggttta tgttgttcct gcattcgttg ggctaggtgc tccttattgg
     6841 gatcaggcag cacgaggggc tatgtttggc ttgacacgtg gtacgacaaa agaagatatc
     6901 attaaagcta ctttacagtc gattgcttat caagtgagag atatcatcga tacgatgcaa
     6961 gatgacactg gaatcaagat tcctgtattg aaagtagatg gcggtgcagc aaacaatgaa
     7021 tatctgatgc aattccaaac agatatcttg aatgtaccga tccaacgagc agaaaacttg
     7081 gaaacaacgg cgctaggggc agcattttta gcaggattag ctgtcggtta ttggaaagat
     7141 acagacgaga tccgcgaatt ttatgaagct ggcaagctat ttgaagttca aatggaggaa
     7201 gagcgccgag aaaaacttta caacggttgg aaaaaagcag taaaagctac ccaagcattt
     7261 gaataaggag gaaatccata tgttttcaaa caagacaaga caagatagca ttcaaaaaat
     7321 gcagcaagaa gaattggatc tgttgatcat cggtggcgga atcactggtg ccggtgtggc
     7381 agtccagtca gcagcatcag gaatcaaaac aggattgatc gaaatgcagg attttgcaga
     7441 agggacttcc tctcgctcga ccaaacttgt tcatggcggt attcgttatc tgaagacatt
     7501 tgatgtggaa gtagtagctg acacagttgg tgaacgtgca gtcgtacaaa gtattgcccc
     7561 acacatccca aaaccagatc caatgctttt gccaatctat gagggcgaag gagcaacaac
     7621 cttcaatatg ttctcggtca aagtagcaat ggacctttat gacaaactgg caaatgtgac
     7681 aggaaccaaa tatgagaatt atacgctgac accagaagaa gtattggaaa gagaaccgtt
     7741 tttgaaaaaa gaagggctaa aaggcgcggg tgtctatcta gatttccgca acaatgatgc
     7801 ccgtttagtg attgataata tcaaaaaggc tgccgaagat ggggcttatc tagtaagtaa
     7861 aatgaaagcg gttggctttt tatatgatgg cgatcaaatc gttggcgtca aagctcgtga
     7921 cctgctgaca gatgaagtga tcgaaatcaa agcaaaatta gtgatcaata cgagtggtcc
     7981 ttgggtagat aaagtacgaa acttgaattt tacgcgtcca gtctctccta aaatgcgtcc
     8041 aactaaaggg atccatttag ttgtagatgc gaaaaaactg cctgtaccgc aacccacata
     8101 cttcgataca ggaaagcaag atgggcggat ggtttttgct attccaagag aaaacaagac
     8161 ttactttggt acgacagata cggattacca aggagatttt acggatccga aagtcacaca
     8221 agaagatgta gattatctat tggatgtgat caatcatcgc tatccagaag caaatatcac
     8281 attgaatgat atcgaagcaa gctgggcagg acttcgtcca ctgttgattg gtaattctgg
     8341 ttctgattac aatggtggag ataatggatc gatttcagac aagagcttca ataaagttgt
     8401 cgatacagta agtgaatata aggaaaacaa agtttctcgt gctgaagtag aagacgtgtt
     8461 gaaccatttg gaaaacagcc gtgatgaaaa agcaccttct acgatttcca gaggtagttc
     8521 tttagaaaga gaaccagatg gtttgctgac tttatctggt gggaaaatca ccgattaccg
     8581 taagatggca gaaggagctt tgcgattgat acgccaatta ttaaaagaag aatacggaat
     8641 agagacgaaa gaaatcgact ctaaaaaata tcagatttca ggtggaaact tcgatccaac
     8701 taaattagaa gaaacagtga cagaattagc aaaagaagga gtagcagccg gtttagagga
     8761 agaagatgct acttatatcg ctgattttta cgggactaat gcccgacgta tctttgaatt
     8821 agcaaaagaa atggcacctt atcctggttt gagtctcgct gagtcagctc ggttacgtta
     8881 tggtttagaa gaagaaatgg ttttggcccc aggcgattat ctcattcgtc gtacaaatca
     8941 tctgttgttt gaacgagatc agcttgatga gatcaagcaa cctgtgatcg atgcaattgc
     9001 tggatatttt gaatggacag aagaggagaa ggcgcaacag actaaacgtt tagaagcatt
     9061 gattgcagaa tcagatctgc gggaactaaa gggggagaaa taaatgaaca gtgatatgac
     9121 tcaaatcatg ggggagttta ttgggacact gatcctagtt ttgcttggag atggtgtgtg
     9181 tgcggctgtg aatttaaaca aaagtaaagc acaggcttct ggctggattg tcatcgcatt
     9241 tggttgggga ctcgctgtaa caatggctgt ctatatttct ggattcatgg gcccagctca
     9301 tttgaatcct gcagtctctc tagctatggc gatgacaggg gcaattagtt ggaatcttgt
     9361 cgttcctttc atcattgcgc aagttttagg agcttttgca ggggccattc ttgtttggct
     9421 ttcttatttg ccacattgga atgcaacgaa agatgaaagc gcgattttag gaacgtttgc
     9481 aacaggtcct gctatacgga attatccagc aaatgtgatc acagaattaa ttggtacatt
     9541 tgtattagtt cttgggttgc tagcttttgg acaaaacgaa tttgcacctg gaacgaacgt
     9601 attcgctgta ggtggattga tcttagccat cggtctttca ttaggagggc caacaggata
     9661 tgcaatcaac cctgctcggg actttggtcc tcgattagca catgcggtat tgccaatagc
     9721 taataaagga acttcagatt gggcatattc atgggtacca atcgcaggtc caatgatcgg
     9781 agcaattatc gcggtaggtg tctattcatt aatggtgtaa atctataaaa catactaaag
     9841 tttgacaacc aaaatgtgtc ttgctatagt taagtggaaa ataaataaga cctagaagaa
     9901 tggggtatgt tttgtgagtg tagctggtat ctaatcagta agtcgaatag ccaataaaca
     9961 aagtttattt ggtgtgctta tgaaacggta cctgtcagga aaacaatatc tttgtattct
    10021 aataaggata ggacatgact tttttaagct gtgttctatc cttttattta tctctaggcc
    10081 cttatttatt ttgggacagg tatctcttca agaaaaggag agaatatgga caacgaaatg
    10141 attagtcaat tacaatttga tcggatcaaa aaggaaatcc aagcaagagc aattggaaac
    10201 tatagcaaaa aacgaatcag cgatatgacg gtctcgacaa acctacaaac tgtcttggat
    10261 cgtcaagaag aaacaagaga agcacgcttg atcttagaaa gcagtcagca tgttccattt
    10321 atggggttgc cgaggatcga tgcactgaca gaacaagtca aaaaagggtt gattctgcag
    10381 ccaaccgatt tgatcgaata tgcggatttt cttagaagca gtcggatgat cacaaagttt
    10441 tttgataaga atcaatacca gacacccttg ttgtttgctt atagcaaaca tcttcctgat
    10501 ttgataaatg tagaagagtt gattgaccag aaaataaaaa ataataaagt cagtgacgac
    10561 gcgtcacgta atttgcgtaa agtacgcaaa caattacaac taatcgaaaa agaaatccag
    10621 tcaaaattac tgaaattctt gcgtcatccg aaaaacaaag aaatgatcca agaagcaatg
    10681 attgttcaaa aaggcgaata ctatacgatt cctatcaaag ccagctataa aaataaaata
    10741 gatggaacga ttatcgatga atcaaataaa agaacgacgg tttttattga accaactgtg
    10801 atttctaaat tgaatgaaca ctatcagttg ttaaaagctg aagaaatcag tgaagaatat
    10861 caagtcctgg cggcattgac tggtgctatc gcagaaaacg aagaagtgat cgatttattg
    10921 atcgaaacga tcacggtgtt agatatcatc tttgctcggg caaaatttag ccgagaaatc
    10981 aatggaagca cacctagaat caataaatcg gaacatatca ttatcaagca aggcagacat
    11041 ccatttttac ctgagcatgc ggtaccattg gatgttgaaa tcggtaaaga ctatcgaggc
    11101 ttgattatca ccggagcaaa tgccggaggc aaaacagtcg tgttgaagac agtcggttta
    11161 ttgactttga tggcgatgtt cggtatgcaa gttccggcaa aagaagggac agagcttgct
    11221 gtctttgatg agattttcgt cgatgtcggt gaccaccaaa atttagaaaa tgcgttgagt
    11281 acattttctg gtcatatgca aaacatcgct gctattttga aaaagataaa aaggaatacg
    11341 ttggtacttt tagatgagat tggcagtgga acagagccaa atgaaggggc tgcactagcg
    11401 atcgcaatca tggaatctat gtatgagcaa ggggcattga tcattgccac gacccattat
    11461 ggagaaatca agaaattcgc cggagatcat gaagattttg tgccggctgc tatggctttc
    11521 gatcgggaag cacttcgtcc taaatatcag ttgcgggttg gaagaaacag gagaaagcca
    11581 agctttgtgg attgctcata aaatggatat gtcgatgaaa ttgatccatc aagcagagcg
    11641 ttacttggcg gaaaaagcct atcggacaca gaaaaaagaa tttcttccta agacagcagt
    11701 tacaaatagg aaggagaata aaaaagaacg tcagctcttt gcaaaaggtg atcggatatt
    11761 tgtcaatgaa taccaaaaag aagcgttagt ttatgaagat ataggtgaag atacgattga
    11821 tgtatattta gacaaaaaaa taatccacgt gcctcgtcag cgtgttcgtc tggtgcgttc
    11881 agcagaagat ctgtacccga caggttacga tttagacagt ctgtttatcg attataaaac
    11941 acgtaaacgg cagcgtgacc tagagcgagg atcaaaaaaa gctcataaag tactagtaaa
    12001 agaaatgcgc aaacgacagg aagaaagaag agtaaacgat gaaaacagca agtgattatc
    12061 tcaaattagc tcataatatc agtgtctgta aagaaaaata aaaacagtga aatcattggt
    12121 tttattcccc gtgtaagcta aaataaaaag aaaagagtgc tgaggaggta gggcgataaa
    12181 atgaaattaa gcacagactg gcgtaaagaa atacaaacga ttccgaatct tttatcgata
    12241 ttccgtattt ttttgctgcc gatttatctt tattttgtgc tgagacagtc attttatata
    12301 gcaggagcgg tcattgttgt ctcaggtttg tctgattatc tagacggggt cattgctcgg
    12361 agatataatc aggtaacaga cttagggaag gtgcttgatc cgtttgcaga taaattgaca
    12421 caactttttc tgattttgtc tatggcatgg tatcgaccgt ggctttggct gttgtttggt
    12481 ctattcttga taaaagaagg ctttatgttt gtagctggat taatcgggct atctaaaaat
    12541 atcaaattaa gtggagcgaa gtggtatggc aaagtagcga ctgctgtcat ctatgttggc
    12601 atgatccttt tgctgctttt cccagaatta ccaacgctgt gggtgagagt catttttgcc
    12661 gtcatcactt acggtctttt acaatctttt gtcttgtatg ctgtcgagta ccgaaagatg
    12721 tttcaaagaa aataaaaaag gggtgtgacc tatgtcgcat tccttttttt gataaataga
    12781 ctcccagtag ctacttctcg ataataagcc aaccgaacca aaaacaggag aagttgtttt
    12841 tgaaattatt tcttgtgatt cggagttaaa tggtaggctc acaacctctt ggtaaacggt
    12901 gttcaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgag cagctatttt
    12961 tccgtttgtt tctctaatac tcaggtcaaa ccgctgatt
//
LOCUS       DACCKU010000083        12989 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.086, whole genome shotgun sequence.
ACCESSION   DACCKU010000083 DACCKU010000000
VERSION     DACCKU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.086"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(5..628)
                     /locus_tag="IVN13_002235"
     CDS             complement(5..628)
                     /locus_tag="IVN13_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012696.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="IVN13_002236"
     CDS             complement(787..2184)
                     /locus_tag="IVN13_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7012697.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="IVN13_002237"
     CDS             complement(2216..3187)
                     /locus_tag="IVN13_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7012698.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="IVN13_002238"
     CDS             3561..3986
                     /locus_tag="IVN13_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012699.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="IVN13_002239"
     CDS             complement(4474..5175)
                     /locus_tag="IVN13_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP7012700.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5191..5631)
                     /locus_tag="IVN13_002240"
     CDS             complement(5191..5631)
                     /locus_tag="IVN13_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP7012701.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="IVN13_002241"
     CDS             complement(5631..6206)
                     /locus_tag="IVN13_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP7012702.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7054..7533)
                     /locus_tag="IVN13_002242"
                     /pseudo
     CDS             complement(7054..7533)
                     /locus_tag="IVN13_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7692..8393)
                     /locus_tag="IVN13_002243"
     CDS             complement(7692..8393)
                     /locus_tag="IVN13_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7012703.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8501..9910)
                     /locus_tag="IVN13_002244"
     CDS             complement(8501..9910)
                     /locus_tag="IVN13_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP7012704.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9958..11823)
                     /locus_tag="IVN13_002245"
     CDS             complement(9958..11823)
                     /locus_tag="IVN13_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7012705.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(11944..12795)
                     /locus_tag="IVN13_002246"
     CDS             complement(11944..12795)
                     /locus_tag="IVN13_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7012706.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         3720 a         2795 c         2115 g         4359 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg
     2041 atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt
     2281 tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga
     2341 tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg
     2401 caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca
     2461 tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt
     2521 aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct ggacaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg
     3421 gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
     4561 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     6301 ggatagacgc cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat
     6361 aaaacaaaaa taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact
     6421 gggatttcgt cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt
     6481 ttattaacga aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc
     6541 taaataaaaa agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc
     6601 tcctaaaaat ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg
     6661 tcactgctgg aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt
     6721 ttgatattgg gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact
     6781 gtataataac tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct
     6841 tcgacataag gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt
     6901 catttcaagg agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt
     6961 atattcgatt ctttctaact ctgattcttt cataccattc ctgtctcaag gtacatctat
     7021 tgtacttttt tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga
     7081 tatgtcttct tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt
     7141 caataataaa atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc
     7201 ccatggtctg gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc
     7261 gccccaaaac atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt
     7321 gcgtgccaag tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat
     7381 aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat
     7441 tttccctaag aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa
     7501 tacagtgatt ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata
     7561 aatggtaatc cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat
     7621 gagaagctat ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     7681 tcttttttta tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc
     7741 gcctccgaat attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg
     7801 ccgatctgtg cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag
     7861 aaatcatcag aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat
     7921 tctttgccaa attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa
     7981 taaatataag gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg
     8041 aaaaaggaaa acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct
     8101 ttccggactt catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat
     8161 cgattgtaag gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat
     8221 tcatctgatg ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt
     8281 tcttctaatt cactttctgt cggcaacatc gaaccgactg gatattttcc tgagaaaata
     8341 tcttctttaa ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca
     8401 ccctttgctt tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc
     8461 acatgtgaga agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg
     8521 aggcaattac ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat
     8581 ttccttcatc gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg
     8641 atgcactgac aagatcgata catccccaag gcgtatagcc catcaaatct acaccgtctg
     8701 ctactgcatt agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa
     8761 taatcgtgcc atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata
     8821 atggtttctg gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga
     8881 tctgccagcc ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc
     8941 cttctgaagt tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa
     9001 atccgatgta gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta
     9061 atttttccgg atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc
     9121 cacgtacttg gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg
     9181 cctgattgac tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga
     9241 tttggatatc cgaacgcaat tcgtggccga tttttacggc tttgctactt gcaacgaatt
     9301 gattatgcca tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc
     9361 cttgacttaa agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt
     9421 tgactttact gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca
     9481 gttttcgatt tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag
     9541 aaatcgtgat cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa
     9601 attctaatcc cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg
     9661 caatcgaaaa acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga
     9721 aacgatcgta atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg
     9781 tccagtcaaa ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat
     9841 ctgcaacgga tagtcctttt ccgtctcgat cataagcccc ttctaattga tttggcagca
     9901 gttgctccac cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta
     9961 ttttacaatt gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat
    10021 atcggcaaat gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc
    10081 tctaattgct tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg
    10141 tttgactaag atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat
    10201 caaaatctcc gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt
    10261 cgtgatctct ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc
    10321 ttttcccatc acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt
    10381 acccgttaaa ggacttttta aggtatgccg tgctgaagta atcggttctc ctgcattcgc
    10441 atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc
    10501 tacgaatgca agaacaaaag caattcctgt agctattact gctactgtca catttgactc
    10561 tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga
    10621 gaaagctttt actccggaaa atccgataat agctccacca atacctccag aaatacaagc
    10681 ggcaatgaat ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc
    10741 aaataacgaa gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg
    10801 caatgctaca gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat
    10861 caatacatca aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg
    10921 catgccgaac atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg
    10981 actaaatcca taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat
    11041 tggtcctaaa gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa
    11101 aatcatttgc agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac
    11161 ccatacggct agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac
    11221 tgggatccca aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata
    11281 cacaagtgcc atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc
    11341 tgtaaaagca agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat
    11401 ttgataagta ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc
    11461 tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat
    11521 atcgatcaga cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag
    11581 accttcctca tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg
    11641 atactgaccg ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt
    11701 atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact
    11761 tttgacattc ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc
    11821 cattgttttt tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca
    11881 gtcacaaaca ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa
    11941 tccttatgat tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata
    12001 aatctgctct gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg
    12061 ttcgacacaa tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc
    12121 ttgctcgtca gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat
    12181 gatacgctga taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat
    12241 actcagtata tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc
    12301 ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac
    12361 acccgtcttt tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata
    12421 aaattttttt gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt
    12481 gatcgcataa tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt
    12541 tagtttatct tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat
    12601 atcagccatc agctgctgga attggttttt agcaaaagaa tctcttaaaa caaattcttt
    12661 ttggaccgct tctggcgaaa tctcgtcccc aatctttttt tgaaaagcta gtccttttcc
    12721 catacaaacg acttcttccc cgaatccatt tttcgaaatg acgacattgt tattcaaaat
    12781 tttatcaatt ttcattttgc ttcgccctct caagaaaaat aaaaaaacct agaattcgcc
    12841 tactgtttct tagctgaatt ctggttttgc ctgccgtaca gtaacaatcc atttcttatt
    12901 caaaatcatc ataccatcga atttttattt atgcaagcct tttcatataa aaaataaaaa
    12961 aactggaaca caagtctatc tagaaaaca
//
LOCUS       DACCKU010000084        12857 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.087, whole genome shotgun sequence.
ACCESSION   DACCKU010000084 DACCKU010000000
VERSION     DACCKU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.087"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IVN13_002247"
     CDS             6..305
                     /locus_tag="IVN13_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012707.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IVN13_002248"
     CDS             378..1739
                     /locus_tag="IVN13_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7012708.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IVN13_002249"
     CDS             1761..2021
                     /locus_tag="IVN13_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012709.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IVN13_002250"
     CDS             2035..2787
                     /locus_tag="IVN13_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP7012710.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IVN13_002251"
     CDS             complement(2881..3486)
                     /locus_tag="IVN13_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7012711.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IVN13_002252"
     CDS             complement(3539..4513)
                     /locus_tag="IVN13_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP7012712.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IVN13_002253"
     CDS             complement(4891..5670)
                     /locus_tag="IVN13_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7012713.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVN13_002254"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IVN13_002254"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP7012714.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IVN13_002255"
     CDS             7161..8558
                     /locus_tag="IVN13_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7012715.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IVN13_002256"
     CDS             8639..9079
                     /locus_tag="IVN13_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP7012716.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IVN13_002257"
     CDS             9227..9553
                     /locus_tag="IVN13_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7012717.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IVN13_002258"
     CDS             9554..10303
                     /locus_tag="IVN13_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012718.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IVN13_002259"
     CDS             complement(10383..10838)
                     /locus_tag="IVN13_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP7012719.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IVN13_002260"
     CDS             complement(11265..11540)
                     /locus_tag="IVN13_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012720.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IVN13_002261"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IVN13_002261"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP7012721.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4122 a         2211 c         2625 g         3899 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttattcgga
    12781 aaaagagcgt gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac
    12841 ggcggaagca gaagaaa
//
LOCUS       DACCKU010000085        12718 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.088, whole genome shotgun sequence.
ACCESSION   DACCKU010000085 DACCKU010000000
VERSION     DACCKU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.088"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            678..878
                     /locus_tag="IVN13_002262"
     CDS             678..878
                     /locus_tag="IVN13_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP7012722.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1446..2009
                     /locus_tag="IVN13_002263"
     CDS             1446..2009
                     /locus_tag="IVN13_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012723.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(2070..2435)
                     /locus_tag="IVN13_002264"
     CDS             complement(2070..2435)
                     /locus_tag="IVN13_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012724.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(2511..2756)
                     /locus_tag="IVN13_002265"
     CDS             complement(2511..2756)
                     /locus_tag="IVN13_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7012725.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2826..3080)
                     /locus_tag="IVN13_002266"
     CDS             complement(2826..3080)
                     /locus_tag="IVN13_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012726.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3244..3567)
                     /locus_tag="IVN13_002267"
     CDS             complement(3244..3567)
                     /locus_tag="IVN13_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012727.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3754..3827
                     /locus_tag="IVN13_002268"
     tRNA            3754..3827
                     /locus_tag="IVN13_002268"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3788..3790,aa:Ile,seq:cat)
     gene            complement(4212..4709)
                     /locus_tag="IVN13_002269"
     CDS             complement(4212..4709)
                     /locus_tag="IVN13_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP7012728.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKAHQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4749..5558)
                     /locus_tag="IVN13_002270"
     CDS             complement(4749..5558)
                     /locus_tag="IVN13_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7012729.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5892..6749
                     /locus_tag="IVN13_002271"
     CDS             5892..6749
                     /locus_tag="IVN13_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7012730.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6742..7644
                     /locus_tag="IVN13_002272"
     CDS             6742..7644
                     /locus_tag="IVN13_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP7012731.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7677..8027
                     /locus_tag="IVN13_002273"
     CDS             7677..8027
                     /locus_tag="IVN13_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP7012732.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            8061..8999
                     /locus_tag="IVN13_002274"
     CDS             8061..8999
                     /locus_tag="IVN13_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP7012733.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            9077..9970
                     /locus_tag="IVN13_002275"
     CDS             9077..9970
                     /locus_tag="IVN13_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7012734.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            10008..10571
                     /gene="amaP"
                     /locus_tag="IVN13_002276"
     CDS             10008..10571
                     /gene="amaP"
                     /locus_tag="IVN13_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP7012735.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10584..10772
                     /locus_tag="IVN13_002277"
     CDS             10584..10772
                     /locus_tag="IVN13_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP7012736.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            10785..11687
                     /locus_tag="IVN13_002278"
     CDS             10785..11687
                     /locus_tag="IVN13_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7012737.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            11719..11961
                     /locus_tag="IVN13_002279"
     CDS             11719..11961
                     /locus_tag="IVN13_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP7012738.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(12217..12588)
                     /locus_tag="IVN13_002280"
     CDS             complement(12217..12588)
                     /locus_tag="IVN13_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012739.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
BASE COUNT         4505 a         2131 c         2388 g         3694 t
ORIGIN      
        1 tttttatttt gaagtatcta ccttatttgt agtgaacaat ttagaacaaa cacaaaacgc
       61 atctactgtt atctaccagt agatgcgttt aaagagaaga acgagaatcg tccatatgag
      121 ggtttccact ccttatgcag atttttttaa ttcttgcact gcttgttcga tcgtaacacc
      181 tgtcgctaca tgattggtat tttctttgtc aaaaacgaca aatagattca aatctgtatc
      241 caaggttact cgatattgta tatctttgtt atagaatgtc atagccattc tcctttcttt
      301 aaatacttgc tgaaaggcaa cgaacgcgtt gataacgaaa ttccatttat aagtatacca
      361 ccaattgaaa ggttttcata gttaagaaat tttaaagtca aaagttgcag ataaaagcaa
      421 ataaacaaga aaacaaaaag tgaatcaatc gcggttattc aactttttct tcgattcgaa
      481 gacaatagaa gacaatgaat tttatatttc attgcattat ccttgtgtta ttagaaaaag
      541 catgatatat taaatgagta atctattttg aaacgtaatt tgagcgatat atctacatag
      601 ataaaactcc ttttgcctat caaatattta ttgcaacgaa aaaacgtaca ttgatacgta
      661 tcaggaggaa tttatttatg aataacggta cagtaaaatg gttcaacgca gacaaaggtt
      721 ttggctttat cactggtgaa gatggaaatg atgtatttgc acatttttca gctatccaag
      781 gtgacggctt caaaacttta gaagaaggcc aagcagtgtc ttacgatgtt gaagatggtc
      841 aacgtggccc tcaagcaaca aacattgtta aagcttaata aaaattgact tttaaccgtc
      901 ttttcgaaag aaaagacggt ttttttgtac gaaaaaattc attggtcgtt gaatgggctg
      961 aatagtagtg tataaaacaa ttttgtggag cgtgaagctt ttatggtaga ggaaaatacg
     1021 aataaagtga tgttgataaa aagattccta tcttgaatat gaatcgagat gtcttgaaaa
     1081 tgaatgagtt tgaccaatca ttatcctatc gaaatttaca gtttcgttcg attggtctgt
     1141 caattaatga aaaagtaaaa aagaatatgt tctaggaaga gggcggcaca tctaaatttt
     1201 taagtaacgg acagttcaaa tatcaagcat tgaaagaggc tatcctactg tttgaaggaa
     1261 tacgaatata taaaaacgac aaagaggaaa aagtagttca ttaaatccct tcaagtgatg
     1321 aaagaatgag atctaataaa acctgatctt tcatctgttt ttttaataac gtaataattt
     1381 ttttattttt tgtgctttcg ccgttattgt agagtgattt agcattctta tttttaaaaa
     1441 taactgtgtt atatattaaa aaaggcataa tagttataag cttttttaaa gaatatttag
     1501 aaattattaa ttttcgtctg aaaacaaaaa aagaaagcgt tattatttta gtctattttc
     1561 agggggggaa ccttatgata aaaaaactat ttttactatc aatattaacg ctagcaatgg
     1621 caggttgttc tgactctggc ttagcaactg aactgatcac aaaatcaact gaagccacga
     1681 cagaggagtt aaaatgtgga aacgatcaca gtgactacag aggcttaaat cgacgatgca
     1741 tgggaatatt aggcagaagc aattgtaagc atcgatgtag taatcgaaga aggtgtagaa
     1801 gaagcaacag aaaaatcaaa agaaagatca aaagaagcaa tctgtgctgt gcatcgtata
     1861 cagacgtcga agatgcggta cttttaaacc acgtcaggag atgttcattt atgtctaggg
     1921 aagttggcga tgaaccaaaa ctagagtacg cttttagttt tgcacgtgag gatgaagata
     1981 accctaaaac ttacgacatt ttagcttgat ctaagatggt taaagctttt taatgaagta
     2041 agaggagata gaaggctttt ctagttatat taggtaagat aaattccttt ttgctccaaa
     2101 tttgctaaac attctgctag ataaacttca tcgtgttccg gataaatcgg cacgttgcgc
     2161 tctacagtag gtaaatcaaa aaatgcttcg catgatttgc cacgatacat aggatttttc
     2221 cactctggtg ctgggctgta accacgtttt tccatttcat ccatgacaag ctgatgatat
     2281 tggaataatc tataaggaga atagtgaaaa acataatcca cagtagcatg tttctttccc
     2341 cagccatttc ctcttaatgc acagcattcc cgatgttgtc ctaaaagctg ttgacgagga
     2401 agctttgaaa tcaatttttc atgccataat cgcatagaaa tgtctccttt atatatagat
     2461 tgttaacctt agacgttaaa tgaaaaaacc agcaccaagg ctggaaaaaa tcacttcagt
     2521 ttgcttgctg tttctgagcg accaattagg taatcaacgg aaatatcgta aaagtcagcg
     2581 atatagatta attcaatgat gcttggaatt tcttctcctt cttcgtactt acgatatttg
     2641 aataaaggaa tacttaacgc gctagaaaca gctacttgag ataaagatcg gccagttctt
     2701 aagaaattca gacgacaacg taaagtatcc agtaaaatag cttcttcgat actcatttaa
     2761 tcacctttca ttatagtaga atagttataa tgactataga aagctacaca tgcaatcaat
     2821 agtactcaat gttttttatt taaacgtatc aaataaagaa tgacgatcaa taaagacaaa
     2881 accagtcctg ataattcttc tataaaaaca ctatcgggtt caaagtagtt gtaattaaaa
     2941 actttccaca taaagtagat agccggaaga gtcaaaatag ctaaataaaa ccgttctttg
     3001 ttccaattca taagataaac aatacttgcg accagtgcga ccgcaggcac ccaaaacaaa
     3061 actctcacca acgtaatcat gtaacggact cctcaacgat tcatataata aaagataaca
     3121 tgttattgca tacatcacaa atgaaatgct aggaaaataa aagaagaata gatcaatgaa
     3181 aaatgaaaaa gcagaacagt tttctgctga taagcaggaa ctgttccgct gaatgtgtct
     3241 tatctatcga gaaaaaatca atggattgat atctaagtaa ttcaatacat ataaaaagag
     3301 gacgatcgca ttcactaaaa aggaaagaat gatgatccca tagcgaatgg aaacatattt
     3361 aacaaatact tttgtttgaa tacgggcata tctttttcct ttatagctga gcatcatgac
     3421 aagaagcaaa agggtaatca caccttgaaa aatcattcca ataaacagaa ttcctgaggt
     3481 ttgataattt aattggagaa aataagctgc tgccgcaact aaatcgaata aaatcgcaat
     3541 aaatggcaaa ataatcactc ctaacattga gaataaataa aagaataaca ttttttctaa
     3601 aataagcaaa taaaaattaa agtaacaaaa tagttatgac acaagtaaaa gaaataagaa
     3661 taacaatatt attttactta tattttgaaa cttgtgagag tttgtcattc gtgctatagt
     3721 tgaaaagtaa caaaaataaa ttttatttat gatgtacctt tagctcagtt ggttagagca
     3781 gacggctcat aaccgtccgg tcgtaggttc gagtcctaca aggtacataa gaggttgtga
     3841 aaaagctgcc ctgcatcaag taataagaac agccaagtaa aaacagcttc ttatgttttt
     3901 acttggctgt tcttattact gcagatgcaa gcttttgaat accgtttaag gaagaggttg
     3961 tgaaatcagc gttttgacct gagtattagc ttaacaaacg gaaaaatagt tcctcatatt
     4021 ttgccgtttg ttaagctaat accgagggtc agctgattga acaccattta agaaagaggt
     4081 cgtgagcctg ccgtttcact ctgtgtcata aaaaagccaa tctactttga tagaaaaagc
     4141 acgtaggact aaaacagttc tgcgtgcttt ttttattgtg tgttaaatat cgtaaatgaa
     4201 gatagatatt tttatgattc gacgatagag acgttgaaat ctttcaacag ctctaaagcg
     4261 gattctccgt gttttctact ggagactaac attggatcaa caaaaaagtc tgcttcagga
     4321 aaagcactct gcaggcctat cagattagag agtacacaca agtttgtttc aatcccgact
     4381 acttcgatct ctttttgttc ttctttgctt tcaaataaag tttcctggat ttcagctaaa
     4441 gtttcaggtg gaatcgtaaa gtaatacttt ttaatcactc gttcattttc ttgtggagaa
     4501 agaagaggga tcaattgcaa atcttctcgt tcttcttctt tgtccttcag ttcaactggt
     4561 atatcccgtg tgaagagaat atattcatta ttttggtgtg ctttagctaa tctttgagca
     4621 atacgttctg ccaattgatc tgactctgga atataaaatt cgctgtttgg atcaagaata
     4681 tgattttgca tatcaataat aactaacatt ttctttcctc ctacattcat atcgtcattt
     4741 tattcttttt aatagaagtt ttttaaaata taagcaacac cagcttcttc attcgtcagt
     4801 gttacttggt ttgcaatttc ttttacttgt cgctgtgcat tccccatcgc aatactcaac
     4861 ccgctgtaat tcaacatagc aatgtcgttt tcttgatcgc caaaagccat gatgtcactc
     4921 gattcgattc caaggtaatc cgccagaaaa acaagtgaat gagctttagt gatacctgta
     4981 ggcgtgattt ctaagaaatg cggatcagag aatactgatt ggataccatc tgcttgcaga
     5041 tctctttgta gcttctgtag tttttcttca ctcttatctg ttaatgctaa tttaagatag
     5101 ctaagagaag gatcttccaa catatttaaa atttgatcaa aagaatggtt ctgtaaattg
     5161 aaatgttcat aataagggtc atctttgact agatttgatt catcgaatgt cagctgatca
     5221 tcttgggttt ctcggctagc aatgatggat attttcgctt cttgagcata gaagaaggct
     5281 tttctcagtg tatcgctatc tatttttgaa cgtctgagaa ctttcgccat ttttccatcc
     5341 ttcatttcag caatcagtga accattggaa gaaatcaaat agccaccgat tcctaattcg
     5401 gaaaaaatat ctttagcaga aatgctgttc cttccggtag cgacggttac gattccgcca
     5461 ttttcatcaa atttccgaag tgcttggata ttttcttctg gtatctttcc atttcgatca
     5521 agcaaggtac cgtctaaatc aatcgctgct agtttcattt tatcttccta ctttcttttt
     5581 acatatctat tttcctgtta ccttaagatt aaatgagaat ttaaaaaaat gaaactaact
     5641 tgctttgtca ttatgaattt tttcaaaaaa cattcttaag aaaaaggtgg ctatatatag
     5701 tagtttttgg tcaatacctt tgaataaaaa gccatgaaaa acgaatagtt agcgcttacc
     5761 gatgcgtttt gtgataaaaa aattttaaaa tattcaatga aaacaaagca tatattttgc
     5821 tttaaaacca aatgaggaat attctagatg tagaacaggt tagaaaaaga ttagaaaaag
     5881 gagtgatttt gatggagaag agatacggcg tagttcctgt ggtaactgct tcagacgaaa
     5941 attatgctcc ttacttgagc gtgatgatcg caactgcatt agaaaattgc aataaaacaa
     6001 gacgcataaa attttatgtg attgacgatg gtctatcaga atatagtaaa gaaggattgg
     6061 aagaaacggt aaataaatat tcaagtaatg caagtatcca attcctgaca gtggaaaaag
     6121 atatctacga agacttccta gtcagtgacc atatcacaac taccgcttac ttaagaattt
     6181 ctttaccaaa cttattagct aaagaggatt ataagaaagt cctttactta gattcagatg
     6241 tgttagtact agacgatatc gtaaaactat atgatgagcc gttaaatgga aaaacaatcg
     6301 gcgctattat cgatccaggc caagtgaaag ctttggaaag attaggaatc gattccgatg
     6361 acttgtactt caattcagga gtgatggtga ttgacattga tcaatggaat aaaaaagaaa
     6421 ttacagaaaa aactatccat tacctcagtg aaaatggcga tcgaatcatc tatcacgacc
     6481 aagatgcttt gaatgctgta ctttatgaag attgggaaca acttcatcca aaatggaaca
     6541 tgcagacttc gctgatattt gaacgtcatc cagcaccaaa tgaaaaatat gaacgtcagt
     6601 ataaagaagg aaatgaaaaa ccttcgatcg ttcactttac aggacatgat aaaccttgga
     6661 atacgttgaa agatcatccg tataccaatt tatacttaaa aaaattagca cacagtactc
     6721 taacgaaagt aggcgagatc aatgaataaa aaagaaatag cagtagtcgc aagttgtaac
     6781 acaaaattcg taccacattt agctgcatta tttgtctcag tattagataa ttgcaacccg
     6841 tctaaatttg ttcggttcta tgtgattgac gatgatatcg attttgaaag taaacagctt
     6901 ttacgcttct cagtgaaaaa cgcacgaatg aactcagatg tggaattctt gaaaatcaat
     6961 aaagaattct ttacaaatgt ggtaatcagc gatcgtattc cagaaacagc ttattatcgt
     7021 attgcgattc cggaattgtt ccgcggcaca gaagttgaac gtatcttata tatggattgc
     7081 gacatgatcg ctttacaaga tatctctaaa ttatggcgtc ttgacttcgg tgattcaatc
     7141 gtagcagctg ttgaagacgc tgggttccat caacgactgg aaaaaatgaa aatttcagct
     7201 aaatcaatgc gttatttcaa ctcaggtttg atgctgatca atgtgaaaaa atggttagat
     7261 gaaaatatta cgcaaaaagt attagatttc atcgaacata atccagaaaa attacgattc
     7321 catgaccaag atgcattaaa tgctatctta catgaccgtt ggttaccact tcatccgcgt
     7381 tggaatgcac aaggctatat catggcaaaa gcaaaaaaac atccaacagc agcaggtgaa
     7441 cgtgaatacg aagaaactcg caacaatcct tacattatcc atttttcagg acatgtgaaa
     7501 ccgtggagca aggattttga agggccaacg aagaaatatt acgaaaaata tgcagggatg
     7561 acggctttcc gttgtgtagc taaattccct aaatatccaa aatatgcaaa agttcaacca
     7621 aaaacagatg tagttgttaa ataatagaag acaacaactg aagaggggga gtaggaatgg
     7681 aaattacaat gtctaaaaat gcagtagaga cgttaattga aaaggttggg gaaaatacaa
     7741 aaatcgcact cgcgcttatc aatgattccg atccatttct aagggacaaa ggtgcttttg
     7801 caaaaggcag cttttttcaa atcattcctt ttgtttcgga atttggagaa tatgcgacta
     7861 aaatcgagca tccattattg gacatctata catctaaatt agagcaaaat tatttcggta
     7921 aacgattgaa catggacttc aacaaacagc ttgacagctt ttcattagaa aatgaaatcg
     7981 cagtactcga ttataatatc aaattaaaga actgcttctt cagctaatag tttcgagaaa
     8041 gtccaaaaag gaggtataca ttgagatact actttgtgga tgaagaaaag aatgtaatag
     8101 aaacagatga aaaaaaatat aattggctcg tacttgatag tgcaaagccc gaagagattg
     8161 agcaagcaat cgaagaattc gagcttcctg atgatatttt cgtaggaacc gaatatccgg
     8221 aagaagtttc tcggatggag cgcctttatg agacgaaact agagcatcca ttttctttgg
     8281 tagtgattaa tctagatcag tcagataatc gtatcgaaaa aaaattaacg ccaatatctt
     8341 ttgtactttc cgataatctc ttgattactt gtatcgatag aaaaaccgaa tttgtggatc
     8401 ggctgctgac aaagcatgga aatcatttaa actcgtttga aaagatcatc acgtatacat
     8461 tattagatat ttacacccat tacgtgaaag aactaagaga gatgaaaaag cgaattgacg
     8521 ctttggataa agcagctcgt aaaacaacgg aaaatgaaga actgtataaa caggcagatc
     8581 ttgaaagaga catcgtatat atcgatcata ccttaagaga tcaacgggaa acaatggatc
     8641 aattatggaa ttctgaaact ttcatcaaac gtttagacga tgaacagcta ctctatgatg
     8701 tccatttgag acagcgacaa acagaaaaaa tgatcgagat ctatcgagat ttattagaga
     8761 gtattggtga tctattcaac gggatgatgg acaataatct aaatcatatc atgaaatatc
     8821 tagattcagc tgcattaatc atttccgttc cggcgttgtt cgcagggatt tggggaatga
     8881 atacaggagg attaccgggt aaaagttctg ctctaggctt tatactcgta gtctttggct
     8941 ctttgatagc agcagtagtt ttaggttacc atttataccg gaaagattat acaaattaaa
     9001 gaatctattt aaaacaggta tcaaacaaag aaatttgttt tggcaataca atttaaaaac
     9061 taggaggaat acagcaatga cagaaccaaa attaatcgac ccgcgaaact tgtaccatac
     9121 ggagaaattt cctaaacaag atcaggatac accagcttta cagcagaata tgattcctgt
     9181 tcctgattgt ggagaagaat cctatgaagg caataatcaa ctagaaaacc gccgtgtact
     9241 aatcactggt ggagattcag ggatcggtcg tgcagcagcg attgcatttg ctagagaagg
     9301 ggcagatatc gctcttcaat tcttcccagg agaagaaaaa gatgcagagg aagtagcaaa
     9361 atacgtgaaa gatgcaggac gtaagattgt cttactacct tttgatcttc gtgacgaaca
     9421 agcacctaaa gaaatcatag ataaagccgt gagtgaactt ggcgggctag atacgatggt
     9481 actaaacgca ggacagcaaa tttcttgtca gtctattgct gagttaccac ttgagcaagt
     9541 caaagacaca tttatgataa acatcatcgc tatgtttgct ttagtgaaag aagctgttcc
     9601 gcatatccca gcaggaggag caatcgtaac aacaacttct gttcaagcat tcaatccaag
     9661 cgaacattta ttagattacg cagcaacaaa agcttcaatc gctaacttta cgatcggttt
     9721 agcaaaacag cttgcaccta aaggaatccg tgtaaatggt gtagcacctg gtccaatctg
     9781 gacaccatta caactagacg aaggacaacc ttctgattcg atccctgaat ttggtcagca
     9841 aagtttacta gaacgtgcag gtcagccagg agaattagca ccagtttatg ttttcttagc
     9901 atcgaataaa gcaagttatg ttacagccca aatttacgga gtaactggtg gagaagcgat
     9961 caacttataa gaagttgatc gtttcacccc tatgaggagg tgcgaaaatg aataaaggtg
    10021 taaaaacgat cggagtgatc ggaacattac ttttgctatc tgtgcttttg tttgcgatac
    10081 tattaagtgc aacctatcca ttgccttttc agctagagaa gtttcgattc ttttccatca
    10141 ctaattcata cgtgcaacaa tatgtatttt ggcttgccgt tgcatttgct attttgacaa
    10201 ttatcacgtt acttgttttc ctgttttatc caaaaacaat cggaaacttt ctactgaaac
    10261 atggtgatgg ggagttgacg ttggataaaa aagcaattga aggaatggtt cgttctcact
    10321 tgcatgaaga tgaattcatc cattcaccaa aagtaaatgt caaagcaacg aaaaacaaga
    10381 tcgccgtcaa tgtaaaaggg gagttgaaac gaacttcagc actcattggt aaaacaggaa
    10441 cattgatgaa agaaatcgaa aatgaagtac aacacatatt gggaacaaaa gagccagtga
    10501 aagtatcggt gaaatacaca ggctttgaag aaccagctaa acgaagcaat gaccgcaaac
    10561 gagtagaata ggggggacca aaaatgaaag aactattttc gacatacaag ctccctatca
    10621 tctttggggt acttggattg atattagccg ttttatggat caccgtgggc tttttcaaga
    10681 ccttattgat cttgatcatg gcagcgatcg gcgtcgctat cggtttttat ctgaaacaaa
    10741 cgaaaatctt agatcactat tttaaatctt aggaggaaac atccatgaat aacaataaca
    10801 aaaataataa caataaaatg aacaaccaaa aaccagtgtc tgcacatcca gacccaacga
    10861 caccaaacca atcaaatggt gtgaacgatg aaaagaacaa aacagcacca gtatctgcac
    10921 atccagatcc agtagtccct aacgttcaac aaggagtaga agagaagaaa catgatgcag
    10981 aaaagcatgc acctcatcat ccaggaactc cagaatctaa agttccagaa caacatgatg
    11041 caatcgatcc ttctaaacct gtggaagagc aaaaacatcc ttctgaccat gcgaaagcac
    11101 aagaaaaact tagttctgac cacacaaacg gacaaggaaa acttggttct gatcatacaa
    11161 ataaccaagg aaagcatgat tctgaacagg aagattcaat gcgcggtgag ctgaattatg
    11221 aagataaagt tgtgcaaaaa atcatcggaa tcgcaattga acaagtagac ggtttattat
    11281 ctgcaaacgg tggtttcttc tctaatgtgg caggaaaact tgtgaataca gataatgtaa
    11341 cggctgggat cgaaacagaa gtcggtaaaa aacaagtagc agttgatatg gatgtcattg
    11401 tggaatatgg caaagatatc gaaaaaatct ttgaagaaat gcaagaagta atcggcaaag
    11461 aagtaaaaaa tatgacacat ttagaagtga tcgaagtcaa cgctaacgta gtggacatca
    11521 aaacaaaaga agaatttgaa aaagatcaag aaacggtgca agacaaagta actgacgcag
    11581 cgaaaaaaac cggtgaattt gcttcgaatc aaacagataa agtaaaatca gcagctggaa
    11641 gcggcgcaca aaaagtaaaa gaaaatacag aaccacgggt tcagtaataa ggaagtaaaa
    11701 cattaggagg aacgaaacat gggatttatt tggtcactta tcgtcggagg aattatcgga
    11761 gcaattgcag gagcaatcac gaataaaggt tcttcgatgg ggattattgc aaatgtcgta
    11821 gcaggtttag ttggatcagc acttggtcaa gcattattag gttcttgggg accaagtatt
    11881 gcaggaatgg caatcgtgcc atcattgatt ggtgccatca ttttagtact aatcgtatca
    11941 ttctttgctc gaaaactgta aaaagacttt taaaaagagg tcgtgaaaaa gctgttcttc
    12001 atcaagtaat aagaacagcc aaacaaaaat agcttctcat atttttcatt tggttgggca
    12061 tattatcgaa gatgcaagct tttgaacact gtttatacgg aataagaggc tgtgacaaga
    12121 cttttgtcac agcctcttat ttatgcgggc aaattcccaa ttagattttt aaaagttttt
    12181 ccttaaatgt gtctttattg atacagagaa gcttctttaa taaaacaatg cctccctaaa
    12241 ttttcttgag ataggaaagg tgaacataca aaataatacg accactttga aaacccagat
    12301 gatccacaaa tggatcgtat gatactgatg aagtaaaaag caatggatca gaaactcaaa
    12361 gagattgaaa aagacaagga agaaataata tctctttttt actcttgtct gttttagcca
    12421 ataatagctc gtaaagtaat ctagacaaac aaatagaagt aaacaaagtt tcgtaagagc
    12481 agcaatcgta tcatgttgct ctgctagacg attgacgagc aggaaaaatg ttggtaatgg
    12541 aatatagatc acgagtaatg aaagaaaaaa gtaaacaata aatggcatac tattcctcct
    12601 aattgtgtga gttcgccctc attttaaaat aacgatattt gtttgtgaag ctttttcgtg
    12661 tcgtaacaaa tgttatctaa tgtattttta aataaataat cattattatc aaaataat
//
LOCUS       DACCKU010000086        12668 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.089, whole genome shotgun sequence.
ACCESSION   DACCKU010000086 DACCKU010000000
VERSION     DACCKU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 12668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.089"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1178)
                     /gene="iadA"
                     /locus_tag="IVN13_002281"
     CDS             complement(<1..1178)
                     /gene="iadA"
                     /locus_tag="IVN13_002281"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP7012740.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     "
     gene            complement(1335..3074)
                     /gene="yfcC"
                     /locus_tag="IVN13_002282"
     CDS             complement(1335..3074)
                     /gene="yfcC"
                     /locus_tag="IVN13_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP7012741.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(3847..4461)
                     /locus_tag="IVN13_002283"
     CDS             complement(3847..4461)
                     /locus_tag="IVN13_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7012742.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(5067..5444)
                     /locus_tag="IVN13_002284"
     CDS             complement(5067..5444)
                     /locus_tag="IVN13_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012743.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(5744..6625)
                     /gene="galU"
                     /locus_tag="IVN13_002285"
     CDS             complement(5744..6625)
                     /gene="galU"
                     /locus_tag="IVN13_002285"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7012744.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            complement(6668..7687)
                     /locus_tag="IVN13_002286"
     CDS             complement(6668..7687)
                     /locus_tag="IVN13_002286"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7012745.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            complement(8320..9324)
                     /locus_tag="IVN13_002287"
     CDS             complement(8320..9324)
                     /locus_tag="IVN13_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7012746.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            complement(9337..10386)
                     /locus_tag="IVN13_002288"
     CDS             complement(9337..10386)
                     /locus_tag="IVN13_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7012747.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            complement(10403..11224)
                     /locus_tag="IVN13_002289"
     CDS             complement(10403..11224)
                     /locus_tag="IVN13_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7012748.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            complement(11211..11990)
                     /locus_tag="IVN13_002290"
     CDS             complement(11211..11990)
                     /locus_tag="IVN13_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7012749.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            complement(12007..12477)
                     /locus_tag="IVN13_002291"
     CDS             complement(12007..12477)
                     /locus_tag="IVN13_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012750.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            complement(12489..>12668)
                     /locus_tag="IVN13_002292"
     CDS             complement(12489..>12668)
                     /locus_tag="IVN13_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP7012751.1"
                     /translation="RDHVYLVAGLNLPLLIELVSRLDAEVETTVLIQQVLTDSKKMIQ
                     FCNERLNQKIEEDEF"
BASE COUNT         3705 a         2765 c         2039 g         4159 t
ORIGIN      
        1 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
       61 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
      121 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
      181 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
      241 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
      301 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
      361 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
      421 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
      481 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
      541 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
      601 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
      661 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
      721 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
      781 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
      841 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
      901 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
      961 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
     1021 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
     1081 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
     1141 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
     1201 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
     1261 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
     1321 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
     1381 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
     1441 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
     1501 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
     1561 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
     1621 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
     1681 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
     1741 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
     1801 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
     1861 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
     1921 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
     1981 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
     2041 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
     2101 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
     2161 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
     2221 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
     2281 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
     2341 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
     2401 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
     2461 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
     2521 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
     2581 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
     2641 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
     2701 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
     2761 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
     2821 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
     2881 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
     2941 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
     3001 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
     3061 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
     3121 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
     3181 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
     3241 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
     3301 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
     3361 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
     3421 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
     3481 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
     3541 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttcttgt ctctttttcc
     3601 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
     3661 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
     3721 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
     3781 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
     3841 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
     3901 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
     3961 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
     4021 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
     4081 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
     4141 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
     4201 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
     4261 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
     4321 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
     4381 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
     4441 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
     4501 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
     4561 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
     4621 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
     4681 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
     4741 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
     4801 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
     4861 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
     4921 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
     4981 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
     5041 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
     5101 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
     5161 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
     5221 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
     5281 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
     5341 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
     5401 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
     5461 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
     5521 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
     5581 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
     5641 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
     5701 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
     5761 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
     5821 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
     5881 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
     5941 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
     6001 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
     6061 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
     6121 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
     6181 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
     6241 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
     6301 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
     6361 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
     6421 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
     6481 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
     6541 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
     6601 gaatgactgc ttttttgacc ttcacttgct gttcctccta aacgacgatt aactattgat
     6661 catctattta acgaagagaa aattcattct ccatttttcc gtcacgtaac atgatttctt
     6721 ttgctgcttt tttgatatct tctccattat acaaaacatt atagattgtt tgagtaattg
     6781 gcatctcaac acctagctgt tgagctagct ctactgctgc ttttgtcgtt gccacaccct
     6841 caacgaccat tcccatgttt tctagaactt cttccagctt atgtccctgt cctaacaagt
     6901 taccggcacg ccaattgcga gaatgaacac ttgtacaagt aacgatcaag tcccccacac
     6961 cactcaaacc gataaatgtc agaggattgg cacccatcgc gacaccaaga cggctgattt
     7021 ctgccaatcc tcgagtcatg attgctgctt ttgcatcatc tccgaatcct aatccatgga
     7081 tagcgccggc accaattgca atgatatttt tcagtgctgc accagtttct acgccaatca
     7141 catcattatt tgtataaatc ctgaaataat cattcatgaa cagtttttgt acataggctg
     7201 ctgctttttg atcaatactt gcagctgtaa ttgttgtgat atcatgtact gccacttcct
     7261 cagcatggct aggaccagat aatacaacaa ttgcctgtct tttttcttct ggaatttctt
     7321 cagccaaaac ttctgagatt cgtttatgcg tatcttgttc tagtccttta cttgcatgta
     7381 taatgattgg cttagtattc attttaccaa taagttcctg tgcgactgaa cgaatcgctt
     7441 ttgttggaac aacaaataaa atagcatctg cgttatccac tgcttctgcc aattctttat
     7501 atccgataat cgattctggg atcgtcaaat caggtaaaaa atgctgattt gtatgatact
     7561 tattgatttc atcaatctgt tcaggtacat ttccccaaat acggacatca tgcccgtttt
     7621 ctgccaatgt ttgtgctaaa gcagtgcccc aagatccagg accaagaact gcgatttttt
     7681 gtttcatgga tattcctcca aataaataat tgataatttc cattatagta tacatttctt
     7741 aaagttggga ctaatttcca ttgtttttaa gtgtttgttt acttctttta tcgtttttac
     7801 ttgaaataat caatgatatg ttacaatagt ctacatttta tttttacact cgttatgctt
     7861 tgcttgttcc taagaggaag cagttttgac aatgttacta ccagcattac tattaggagg
     7921 ttaggaaact tctcctagtg agtattgatt taaaatacat aagagacaga ttgaacattt
     7981 ctaaatctgt caggtcctgc aacggacaga ttgtctctgt tgacggttga cataggaact
     8041 tttgttttag tcaacattct ggatacaaaa cgctatgtaa actggcgcaa ctcgttgtac
     8101 ttggactttt actacggatc catgcaacaa ctaaatagta ctattgcata acaaaaaatg
     8161 gttggcaccc acatgtccca ttaaacagat tataagttga actgtagata atgcaaaaaa
     8221 tgtgttttat aacgtaattg atgccaatat tttaaaaaaa taaagggagc agttatcgct
     8281 gctctcttta ttttttatat tctttttatc cagtctatcc taataatcaa actgacgata
     8341 gtagcggcgt atctctaatg gatggcagtt caactgctcc acatgagcat cgatgcgttg
     8401 tgtcactaca tgcatcaata agaaagacaa aacacgtccg cgatattttt cactgatacc
     8461 ttctactgga tagtcttttg tgtctatcaa tgtataattg ctgcaaattt ttggcaaaag
     8521 tttagccaca cgttcagata acactctctg ttcatcttcg cctaaaaata atgtgactgg
     8581 ggttgtttct tctacgatct ctaacgtccc gtgaagaaac tcggcagcat ggatagattt
     8641 cgatggaagc cagctttgtt cttcccagta acacatggca tatgaataaa cagctcccca
     8701 ttgattccct gctccaataa aataatgcat actatcacgt cgatgttttt ctgcaaatga
     8761 taagccaaat gcatctgctt ttttctctgc ttccactaac cctgttggta aataacgttc
     8821 gagctgttca tagtattctg aataagcttc gaattctcca ttcaaataca tcaatcgatc
     8881 tgctgccata aaaaatttga tttgttctgt tccaggagct ggataagtga ccacaaaatc
     8941 gcatagctga gacaacttac tgttcgcttt atcgataaat ccaatcagtg tcgctcctac
     9001 tttcttgatt tgttctactg ctttcactac ttcttgagtt gtaccggtaa cagaggacaa
     9061 aataacgata gaatcttttg ttaatctttt gtttccagta gtgtaatatt ctgcagcatg
     9121 ctgtacaaag acaggcaagt tgcttttccc attcatataa gtgacagctt gcatcgagga
     9181 agcatacgtt ccaccgattc ctagataata gacagcgtca aatttttccg cataaagttt
     9241 atcgatgatt tcttctactt gcggacgtaa agctagtgcg ccttttattg ctttgatttg
     9301 ttcttcttca ttgaatttca gcatgatttc cacctcttat aatttacttt tcaagactgt
     9361 atcaaagtct tcaaaaatct ttttgttcaa gtcaatacct ttagctactg cggttttata
     9421 agcgaatagt tgaaacggaa cgacaagcgc taatacagac agatattcat cacattctat
     9481 tgctaatcca agtgttttct cgtttcttgc tttttttgtg gtaatggtga gagtctctcc
     9541 aacgtaccga ctcatatatc tttgcaactt ttgcgaacgt tccttattta cactttcccc
     9601 ttcaataaag aatagcaaat gcgaagcatc tgcctccagg tatgggccgt gcatataagc
     9661 ttctaactca aatccttgtg acggaacgcg gattgtttca gtcaatttcg tctctgcttc
     9721 tttagctgtt ccccagtttg gaccatatcc aatggtgacg aaacgctgtg ctaaacgaaa
     9781 taaactttga tatccatcaa aaaattcttc cgttttttga ataatcgctg gtaaatggtt
     9841 gattattttg cgcaactgtt gcatgtagtc ttgttctatt tttttgctga ttttattttt
     9901 acttattcca attcctatcc ctaagagtag taactgtaaa acagtcgcag aatagccttt
     9961 tgtaacgaag cctactgatt ctaccccggc acctaaatca ataatttgtg cagcttctaa
    10021 agcaagtgga ctttgcacgt catttgtgat agcaacaaca ggtaaccctt gtttttgtat
    10081 catttttact gcttcaacag tagaagcact tttcccgctt tgtgacacag caatcacagt
    10141 atcgattgaa ggagaaagtt ttccataatt gaaaaaataa aaaggttctt gaatatcgac
    10201 agtaagatca ccataagatt ccaatgctgg cttcgctgct aaacaagcgt tataagatga
    10261 acccgttgct aagatcagta aattcactgt ttttttcata tgttctcttg tcgaaaaatc
    10321 attctttttc aagatcgtac ttagtacttc cttttcttca tagatataat cttgcatcgt
    10381 aaacattttc tctgctcctt ttttaaaaaa tccctatcca cgaaccaaaa attccaaaga
    10441 aagctagtcc gatcaagatc gttaatggtt tcacttcttt cttcaataga taaaacacta
    10501 tgccaaatgc tccaagacat aagatatttg gaacgatatc atcaaaaata ctttgaacag
    10561 tcacagcatt ttctcctgaa ccgattttta aaggaatatt gatggtgatc atcgtggcag
    10621 tcattgctcc aacgaccatc agtccaataa tagacgcgcc taatgtcaaa ctttccatca
    10681 taccgtttgc ctggatcttt ttcaaaaaac ccgttcctaa gcgatatccc caacctgttg
    10741 ctagatagcg aaacagaatg tgcggaatat tgaaaatcaa aatgaacaag atagggccta
    10801 aaatatttcc ttgcatggct aaagaggtac ccacacctgt ggcaatcaat cgcaaagttc
    10861 cccaaaagaa agaatctcct aaccctgcaa gaggtcccat caaagatgtt ttgatactat
    10921 cgatcgcagt cacatcaaat tcaggatcat ccttattttc ttcctccatt gcagccgtga
    10981 ttcctaaaat cagtgtaacg atatgtgggg tcgtgttaaa aaactctaga tgccgtttca
    11041 aagctgctgc catttcttct ttcgactgat ataacttttt caaaatcgga atcattgcat
    11101 aaacataagc catattcatc tgacgttcat aattccaaga aaactccatt tgaaaagaac
    11161 gccaaaatac gtgatttagc tcttttttcg ttattttgct gtttttgatc tcagaattct
    11221 tcatcttctt ctacctcccc tgcagttgtt tgaagtggta gttgacggac attcataata
    11281 ttgactacca caactgctaa aatcgcacca aaaatagcta ctccggtcac aggaatctct
    11341 aagtaagaag ctatagcaaa acctaagaaa aaatatggtg caacttgttt gttgatcagc
    11401 aaacgagcaa gcatcgcaaa accaagagct ggaatcaacc ctgtagctac tgacaacccc
    11461 ttttgaacga agtcaggaat catatttaat agatttccga tcgtattgct tcccaccata
    11521 taagaaacca ttaccacaac accaagcatc aaggaaagac cgataccggc aagcaagtgc
    11581 atatgctcga ctcctttata atttccttct tctgcataat tgtctgcttt atggttcata
    11641 attggaatca aaagccctaa gtaaatattt ttcaatatca atgtcaacgt agcaatcggc
    11701 aaacctagta aaagcgcggt ttctgtaccg gctccagctg taatagcaaa agccgtccct
    11761 aaaatcccac cggtcacaac atcaggtgga atcgaagcac ctactgagaa cgatccgata
    11821 aatgctaact ctaaagttgc gcccataatg atcccagttt ttacatcacc taacacgatt
    11881 cctgtaaaca gccccgtcac aatcggacga gaaagcaacg atgtccctag tgcatattct
    11941 gattgtgcga caaatgcaac aattcctaat aagactgctt gtacaatcat tgaggttctt
    12001 cctttcttac aaaacttctc ttacatccac ttttttatct gctggtactt gacggatttc
    12061 tacttctatt tgtttgtcaa ttaatcgttt caagcgttcc tcctcttctg gtaaaatatt
    12121 taccgctttt ccgatgctgc gagtaccagg ttttgccttg atccctccta agttgatggt
    12181 tttgatttct ggatacgctt ctgccagctt atatgcatcc tcgatcgatt ccactacgat
    12241 gaataactta tatttatctg tcaccccgct tttcaacgcg gcaatcgagt catcaattga
    12301 tttgatcact agtttgactc cttgtggctt ggctaatttg atcgttgtct ttcgtatctc
    12361 attcgtaggt acatcatcat tagcaatcaa aatacagtct gcgcctattg tttgtgtcca
    12421 tgaaaaagct acttgtccgt gtaataaccg atggtcgact cttgtcaata caatcatttt
    12481 tcttcctcct aaaattcgtc ttcttctatt ttctgattga gccgctcgtt acaaaactgg
    12541 atcatttttt ttgaatcagt tagcacctgc tggatcaaaa cagttgtctc tacttcagcg
    12601 tccaaccgag atactagctc aatcaatagt ggcaaattca atccggcaac gagatagaca
    12661 tgatcccg
//
LOCUS       DACCKU010000087        11639 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.090, whole genome shotgun sequence.
ACCESSION   DACCKU010000087 DACCKU010000000
VERSION     DACCKU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.090"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            129..992
                     /locus_tag="IVN13_002293"
     CDS             129..992
                     /locus_tag="IVN13_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP7012752.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1121..2743)
                     /locus_tag="IVN13_002294"
     CDS             complement(1121..2743)
                     /locus_tag="IVN13_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP7012753.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2992..3513
                     /locus_tag="IVN13_002295"
     CDS             2992..3513
                     /locus_tag="IVN13_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012754.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3699..5429)
                     /gene="spxB"
                     /locus_tag="IVN13_002296"
     CDS             complement(3699..5429)
                     /gene="spxB"
                     /locus_tag="IVN13_002296"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP7012755.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5594..6412
                     /locus_tag="IVN13_002297"
     CDS             5594..6412
                     /locus_tag="IVN13_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP7012756.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6515..6712)
                     /locus_tag="IVN13_002298"
     CDS             complement(6515..6712)
                     /locus_tag="IVN13_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012757.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6881..7459
                     /locus_tag="IVN13_002299"
     CDS             6881..7459
                     /locus_tag="IVN13_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP7012758.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8504..9007)
                     /locus_tag="IVN13_002300"
     CDS             complement(8504..9007)
                     /locus_tag="IVN13_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7012759.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9366..10391
                     /locus_tag="IVN13_002301"
     CDS             9366..10391
                     /locus_tag="IVN13_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP7012760.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10738..11616
                     /locus_tag="IVN13_002302"
     CDS             10738..11616
                     /locus_tag="IVN13_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012761.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
BASE COUNT         3604 a         1984 c         2259 g         3792 t
ORIGIN      
        1 ccacttctgt cacaacctct tattttgatt agaacttgcg aaaataaacc ggttaaagtg
       61 tgtctagcgc aagagatttg ctataataga cgataagggt aaaatgtaac gagaaggggt
      121 tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga tctgccatac catgtattag
      181 caaaagaaaa agttgatttt ttatcaatga atctccaatt gaatgatcaa gagttagtag
      241 atgacctcgg aaagacattc aattatgacc gctacatgaa tcagctgaaa aatggtgcga
      301 tgcctacgac atcacaaatc aatgttggcc gatatttaga atttttccgt tcttatgcgg
      361 aaaaacagat tcctgtttta tatgtggctt tttccagtgg attaagcggc tcttacagta
      421 gtgcgcttca agcggttgaa ttgctgaagg aagaatatga agaccctgag atctatgttt
      481 ttgatacaaa agctgcaagt attggggaag ggcttttagt attggaagct gtaagattga
      541 aaaaagaagg taaaagcatc gaagaaaccc tagagtggct ggaaagtaat tatttgaaag
      601 tccattcatg ggtaacagta gacgatctga aatatctaga acgaggcggt aggatttcca
      661 aagcaagtgc tactcttggc agtctattga gtgtaaagcc tattattacg gtggataaag
      721 aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa caagtcgatc cagacaatcg
      781 tcaatgagac ggtaaaaaca atcgttgcac cacttgatca aatcgtttat gtggcttacg
      841 ctggggatct agagagcgcg gagaaggcaa aacggctttt ggaagagcag ataaagatga
      901 aagatgtgaa gctttatccc ttaggaccaa cgatcgccag tcacacaggc tatggctgta
      961 tcgcgatttt ttcgatggga atcagtcgat aaaagcagta actcattctg taaaaaagcc
     1021 cagaaattga ttgtaacaaa aacagcgact cttaaaagat agatgagcat cctatctctt
     1081 agaggtcgct gtttttttat tttttaagct aaataggcca ttatgcccat aactcgttca
     1141 attgtttttg aatatctgca ttttccaaaa attcatcata tgtcgtatca gctcgatcga
     1201 tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa tgtttggata aattgatggt
     1261 catgtgacgc aaataagatt gagccagtaa atgccatcaa gccgtcattc aatgctgtga
     1321 ttgattctaa gtctaaatgg ttcgttggat catctaatac aagtacgtta gctttcgaaa
     1381 gcatcatttt tgaaagcatg caacggactt tctctccacc agataaaaca ttcactggtt
     1441 tcaatacttc ttcaccagaa aacaacattc ttcctaagaa gcttcttaag aaggtgttat
     1501 catcttcttc tttacttgca tattgacgca accagtctaa aatcgttaga tcagtatcga
     1561 attctttcgt tgtatccttt ggtaaataag aattacttgt tgtcacaccc caacgtactg
     1621 ttccagtatc tggcgtaatt tcacccatga taactttgaa tagcgttgtt gttgttatat
     1681 cattttctgc gacgaaagcg accttgtctt ctttgctcaa attaaaagat atatcatcta
     1741 aaattttctt tccatcaatc gttacactta cattttctac ctgcaataag tcgtttccaa
     1801 tctcacgttc aggtttgaat ccaacgaatg gataacgtct agaagaaggt tggatatcgt
     1861 ctaaagtaat tttttcaagc atttttttac gtgaagttgc ttgttttgat tttgatgcat
     1921 tcgcactaaa gcgggcaata aattcttgga gttctttgat ttgttcttct tttttggcat
     1981 ttgcgtttgc ttgaagttta gccgctagtt gactagattc caaccaaaaa tcataatttc
     2041 ctacataaag tttgatttta ccaaaatcta gatccgccat atgtgtacat actttgttca
     2101 aaaagtgacg gtcatgggat acaacgatca cagtattttc aaaattgatc aaaaattctt
     2161 ccagccaatt gatcgattgg atatccagac cgtttgttgg ttcgtctagc aaaagaacat
     2221 caggtttacc aaatagtgtt tgtgctaata acacttttac cttttgtcca ccagttaatt
     2281 cactcatttg ctgatggtgc aattcttcag ggatgttcag accttgcagt aaaacagctg
     2341 cttctggttc agcttcccat ccattaagct cagcaaattc tccttctaat tcagctgctc
     2401 gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat cgcatctttt tctttcagca
     2461 cttcatacag acgcttgtgt cccatgatga ctgtttcaag tacagtatag tcttcatagt
     2521 cgtaatggtt ctgtttcaat acggccatcc gttcatctgg tcccatagta acggaacctg
     2581 tggaaggctg cagatcccct gataacactt taaggaacgt tgatttacct gcaccatttg
     2641 caccaatcaa gccatagcag tttccggggg taaatttaat attcacatca tcaaatagtt
     2701 tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat caattttttt cctcactttt
     2761 atcattatta gttttgacaa gtcttataga attatatagg ggaaaggtaa aagattcaag
     2821 gcaatcgaac attctttgtg tttcacgtga aacagaatga aaaaaataaa aataaaagcg
     2881 gagcgaaaat agctactata ggatttatta gtttgcaaca gcataatttt tgacgttatt
     2941 tataagaggg agtactataa aactgtaaaa gaaatgaaag aaggattgaa aatggatgaa
     3001 aaaagaacca tcgactgggg ctcattgatt ttgggaattt tatttgtact tgtctcattg
     3061 ttatcatttc aagaccctgt cggaaattta gttgcaattg tagtagtctt tgcgattttt
     3121 gcctttatta aagggatatt tgaactgttt gtacggaaca gattaaaaga gctaacagga
     3181 tataaaggaa agatgccctt aataatcggg atcatcgata ttctagttgg cgtctttttc
     3241 ttattcaata ttggtgcagg agtcgcagca ttgccttttg tttttgcagt atggtttatt
     3301 gctgattcag ttttagcgtt atttactgct gacttggcaa gaggagtaag tgaaggatat
     3361 tattggttta cgatcatcgt caatatttta ggaattttat taggaatttt cttattgttt
     3421 aatccaattt cttctgcgtt gacattaagc tttttagttg gattctattt tatgttattt
     3481 ggtatcacac atatcgtcta tgcatttaga taagtaaaat caaacaaata aaaaagccgg
     3541 cttccgatta cagttttact gttgtaattg gaagccggct tttttatcat ggacaaatat
     3601 gcacgaggtg atttttaggt gtcgagacaa atttatgcaa aaatgtgaga agctatatac
     3661 acaaatttta tcttaatact cgaagcatag cgcagttgtc acaacctctt ttcatataat
     3721 tcattcaata ctggcaaatc atggacttca tatttttctt tgaatgcttg gatttcttct
     3781 tcagaatatt ttcttggatc taaatgcaga tcttctactg gcaatgggcg cttgttattg
     3841 atcttcacat cgattactac aggacgatct gattttgcag ctgcatcaaa ggcaaatcga
     3901 agttgcgagt attctgtgac tgtaaagcta tttgccccga gcgcttctcc aactttaccg
     3961 aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt ccgtatcttc ttgttctgct
     4021 tcgatgaaac caaaagaatc attcgaaaag acaacattaa taattggaag ctgatatttg
     4081 acttgtgtaa tgatatcttg catgaccata gcaaacccgc catcaccact taaggtaaat
     4141 acttgttttt ctgggtagct tagttgtgcg gcaattcctc ctggcacacc attacccata
     4201 gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt tcatattcaa taaacgaata
     4261 gaatggatag tcgtgttacc gacatctgtg acgaaaatcg catcgtcttc agcaatacga
     4321 ttgatttctt tatagacagc ctctggacga atcggttctt cctctcgatc ttcaaaagat
     4381 ttcaaccaat ttacccattt ttctttattt tcttgatttg ctaggtacca gctatcagca
     4441 gggcgagcat cccctaactc gacaagtctt tgcaaagctg tgtttgcatc accgaggatc
     4501 gacaaagatg tttcatggcg tcgtccaaat tttgacgcat cgatatcgat ttggataaat
     4561 tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt cgctaccgac aaataagatc
     4621 agatcggctt ctgccaatgc ttcatttccg ggtttagtag cgacacgtcc agcaaaacca
     4681 agaaagtttt cataatcatc aggaataatt ccttttgcta gaacggaagc gacaacagga
     4741 actgaaaaat gttctgagaa ttctttgatt tgcggaaatc cgtttcgcgt tccttgacca
     4801 acataaagaa ctggtttttt tgctttttca agataaggca aagcagctag caaatctttt
     4861 tcttctggta atatcacact tgttttgtga gtgtgagcgg tggagaaagg ttgttcgtca
     4921 atttcttcaa aaccaagatc aacagggata gtcacgatag ccacgccttt atgctcatag
     4981 gcggctttaa tggcttcatc tactacatgc ggaagacttt gtggtgtcat gactgtacga
     5041 ttgtatacgc taacatctgc gaaaatagga ttttcattta gttcctggaa agagttatag
     5101 ttcatggatg tagaagcaac ttgtcctaag aacgctacta gaggcacatg gtccatctgt
     5161 gcatcataca aaccattgat caaatgtgtc gcaccaggtc ctgcagagcc gaagacagcg
     5221 ccgactttcc ccgttaattt tgcatctgca gcggctgcga gtgcgccgac ttcttcatga
     5281 cgaacttgaa tgtagttgat ttcggttttt tcttgatata gcgcatccat aattgaattg
     5341 aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc agctttcaag aacttttacc
     5401 attgcgattc ctgcattgat ttttgacatg gtgtttcact cctttagcaa tgatgataca
     5461 taaatttttt gtatctagag tatagaactt tttatcgaaa atcccaacta attcgtcttt
     5521 tcacaaaaat gtagtcatgt ttttgggaat atgctagagt agtacataga tagattcaag
     5581 agaggggaat ggcatggttg aatggctatt gagacttgaa ccttggcagc aagcacttgc
     5641 tgggacagct tttacttatt ttatgacagc tcttggtgcc gggttggttt ttttctttaa
     5701 agaaatcaaa aaagaagtgc taaacttgat gttaggattt gcttctggtg tcatgattgc
     5761 tgcaagtttt tggtctttgt tagatccggc tattacaaaa gcagaagaaa atggagatat
     5821 tgcttggcta gttgtaagta ttgggtttgg tttaggcggt gtgtttttat atatggcaga
     5881 taaaacactg ccccatatgc attttggacc acaacatgaa gcagaaggat tgcctaccca
     5941 tttaaaacgg acgatcttgc ttgttttttc tattacattg cataacattc ctgaaggttt
     6001 agccgtgggg gtagcattcg gtgcagcagc aactgcggat aatccgacag ccgctatatt
     6061 agccgctgtt tcggtggcgc taggtattgg tatccaaaac ttcccagaag gagcagctgt
     6121 atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag gcctttgttt acggacaagc
     6181 ttctggaatc gttgaaccaa ttgctggagt gattggagcg ctactagtaa cgaaagtgga
     6241 gctgctgctt ccttatgcac ttgcttttgc cgcaggagca atgatttatg tcgtggtgga
     6301 agaattgatc cctgaagcac agcagacatt gtccagcaaa cgtcattttg cagtttttgg
     6361 cgtcatgagt ggatttatca ttatgatgat tttggatgtg gcattaggat aaataaaaaa
     6421 aatatatatg atatatcaaa tagggccgcg aacttatatc gcgaccctat ttttttagta
     6481 agttatatca taatgaagtg aatggtatca tacattaata atagtttttt ctatgtgtat
     6541 ttcctttgat ccagcgaaca agccagatga taagtgcgat tgggattaga actttgaccg
     6601 caaaccataa aagcccgctt aggacactga caaaaagact gatgacgaag ctagctgcaa
     6661 ttagcactaa aagaaccatg aagatattta agaacatatt tttttgacgc atacaaaacc
     6721 ctcctcttgt tcattcttta ctatagaaaa agtataacag ggattgttta aaaacacctc
     6781 caactgaaga ctgattttta cttcatctac ttaaatgatc aaacaattgc tttttctctg
     6841 gtttatcatc taaaatagag cggaaaagga ggagaatatc atgcctaaaa atcgtcttga
     6901 agcatttaca gatgctgtaa tcgcgattat catgactatt ttagttttag aacttcatga
     6961 acctaacgga gacacgtggg aggcgctcgc tgcattaggt taccgatttt ttatctatat
     7021 tgtcagtttt atcattttag cgatttattg gaacaatcat catcatttat ttcaagttgt
     7081 acataagata aatggacggg tcttatgggc gaacagtttc tttgtttttg cgctttcgct
     7141 ttttccgttt gcaacttcat ggatcagtga gtacatcaat agcctggctc cagaaatgac
     7201 ttatggtttt gttaccttga ccgctaatat tgcgtattat atcttggcaa aagaacttgt
     7261 caaagcaaat aaaggccacg agaaagcatt gaaggcgatg ttgaataatt acaagaaatc
     7321 gtatctatcg attggtttga atgtgttagg aattgtctta gggtatttag tagcgccagt
     7381 agccgtgctg atcgcaaaca tcgtgattct agctgcttgg atcatcccaa gtcgtaccat
     7441 cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt aagagaaaaa tatagaaaaa
     7501 ataaataaac gatcaaatat ccgagtgata ctagtattca aagagggatg cagatggatc
     7561 aaaaaagaat tcatgtagga ctgattacta gttttgtcac gatgcttatt tatttggatc
     7621 aaggtcattt gacttggaca gtagttggtg tcgtaggatt tttgacatta ttcacagcaa
     7681 taaaaaaata aacatcttta tgtaatctga tgttctatct atttaagaaa agcatgttta
     7741 cagcagtatg attctgttgt aaacgtgttt ttatttgtgg aaaatttaat ttttagaaaa
     7801 gtgtatcgaa aaatagatag ttagtttttc cacagtggat aacttcgtaa gagataaaaa
     7861 taaaaaatta gtcaaagaag ggcgtataga tttcttttga taggagatat acaacagaaa
     7921 tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt gtggtttttg gaataaaaaa
     7981 ataacatatc tcattttacg cttttgttat tttatactta aaaaataacg acacagaaaa
     8041 taccgtgtct cagatttctc tactacttct aaaaactact gtttttttcc tttgaaatgc
     8101 cttcaaaaag taggatcagg cagaatatac tggcagaatc aaggagaagg gagcctaaat
     8161 aaggagaaaa accatagaaa atataaagta attacttata aaacaagctt tttatagaaa
     8221 aataatatac ctgagtaggg tataaattaa gacggaattc ctttctagat tgagatattg
     8281 actaggaaga atgttaagat tttaagcctt taagctaaaa aaaaaccctt tttgttaaat
     8341 aaggggaata tagcctgtca gtttcaaact gcaagtcgtc ctgctgcaat aatatagtag
     8401 attagagatg aaaatagatt tttttaaacc aatgactgaa taaaaagacc gtcatagata
     8461 cacattggaa tgtgtctatt acggtctttt tttacgaacg attttattga agacggacag
     8521 ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa taaaatgtat cccaaaataa
     8581 tggctagacg gtctaactta tttgtcgata gatgtttgtt tagaagttga taggcccata
     8641 aacctatcag tcctcctgtt gtattagtta tcaaatcagt gatgtccgct acgccaatac
     8701 cgaataggaa ttgaatcaat tctgccaaga agcttacaag gaaaataatc cctaattttt
     8761 tagaaaaaga aagtctcttg aaattgacat tcaaaagcac cccaaaagga aaaaagacaa
     8821 tcaagttata agtggtctca cgtaataccg tcgtattttc aaaagaaaaa gtagaaaaag
     8881 ggacgagatt gacattgctg taagaataag tgagaaattg tggaagatta aatgatagct
     8941 tgaataaaat caaccataat agaaagaata aataaatccc taataaaaat tttgatacat
     9001 accgcataga catctcctcc atttaaaaag tataccatcg aaaaccgctg aaaaatcata
     9061 aaaaagtatg aagtttaagg accaacgtag ttatcgtacg aaaattagaa gctttttttt
     9121 gttgggaatc cggtttcttt aacaaagcta agatagcgag ttgcttggaa aagttgctat
     9181 cttttttctg tcaaaacgac tcggaatgtt cgttttttgt tctaatttct ccaagttgtt
     9241 tactttttga tactaagttt atgaaaaatc aagaaatagt tgttatacta ggtaatgctg
     9301 acaaacggga ttggcatttc atcttggttc agcttttttt ccaattacta ggaaggaggg
     9361 atagattgtt caagacttat tttcgtttca ataaaataca agataatcct tttcgtatca
     9421 tgaatctgat gctacttatc tttattgtat tatggttagg ttacataaat catgattttt
     9481 tgatttcaag tttttcatca ttagggatct ttactttttt ctattatcaa aacataccga
     9541 tgaaatattt gctgaaacgt atgtttctga tcggttgcgg actgttggtt gctttcgttt
     9601 tggggatctt atcgacttat gttttatggc ttgaaccatt tgccgtagct ttggttgctt
     9661 tttccagcag attcgtgttg cgattattcc atatttctaa accaggtggc ttgttttttg
     9721 cgatgctttc tgccatggga acgagcatgc agctgccaat cgctcaatta ccgatcgtct
     9781 cactttattt ttttatgggt gtcgtttttg ccctgattgc ggcagtgatt acaaaattac
     9841 tggattcacg gcctgagcag acaattgaaa aagcaacttt aaaagaaaga tttcacgaag
     9901 agcctttagt gatcattgat tctgttttct acagcgctgc cttatttctt tccgtttatg
     9961 tcagtcacgg cttgaatctg cataatcctt attggttgac cttgtcatgt gcatcgattt
    10021 tgctagcaga aaacttggat gcgatgaagc atcgacaagt ccaatatctg attggatcga
    10081 tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc gtttacgcag ttgcagacca
    10141 tttttctgat cacttttcta tatggaatcg cacaatttct agttgctaga aattatgcag
    10201 ttgctaatat ttttcttaat ccgatggcgt tgatgctatc tacacttatt cgtggagcat
    10261 acttgatttc tttgatcgaa tatcgttttt tagggatcgt cataggaagt ttcatcggtt
    10321 taggtgttgc gtgggtaatg acggttggtt tgcaacatta tttaacggtt gttcgtcaaa
    10381 aattagaata gaaggacaaa tatatgtgcc aaaagaggtt atgacaagat ttttgtcaca
    10441 acctctaatt caaggtgttc aaaagccaga ttctcagcat taagtcaaat tcacacaaaa
    10501 atgtggaaag gaactttcaa aaaaatttta tatttattct atcaaaaaat tttatattta
    10561 ttctatcaaa aaattttgtt tatgatggta actgaataaa ctttcctgag atggtcaatg
    10621 ctttccttga aagtcttaca gatattcttt cttcatagaa agtttaagtt ttatagttag
    10681 tgtacaataa aagtatgttt ataccatatg gtgtccggat aatcagtcat ttaaacaatg
    10741 aggttcaaag atatgaaaag gggaaatttg atgaagaaaa taagcaatga aatccaaatt
    10801 ctggcagaaa ctttaactct tcccaaagtc gttatagagc aaatgattgt acaagcaaaa
    10861 aacatccttg atgaggtgtt tgagcaatat agtcatgaac taatggatcc taataaagca
    10921 aaggcgactg caaagaaaat agacaaagtc tatgaagagg aaccttttgc agcattagct
    10981 atttatctat atgcagcttc tcaaagttgg aaaaaaattt atcaaccatt gaagattcca
    11041 agaactatct atttggcaac aatgaatgct tttactagat ttattcatga acatttccaa
    11101 gtaacaggaa gctatcgctt tgaccgtggt ttttggattt ggcattacac tagtggcttg
    11161 atctttcgga ttggtgaatt ggagtacgaa atgacatatt ttccgaaagg gtacacggat
    11221 ctaagattag aaggaaagca gtgcctttct cttcatattc cttccgatgc agatttatct
    11281 cagaataaga taagagatag ttacgaaagt gccattcgtt tttttgaact ttattttcct
    11341 gattaccatt accaagtgat gtacacagat acttggttac tatcacccaa tcttactaaa
    11401 tggttgaaaa aagagtcaaa aatctgtctt tttgcagccg attatcgatt attatcagtt
    11461 gatgaacagg acgatagtgg agtcccttgg atctttggac gagtagatgc ccaaatacat
    11521 gattatccag aaagtacgag tttacagcga caagcaaaag agcaattgct tgcaggcgag
    11581 cacattggct ctggcttagg tattcttact tcataagttt agtacttcga aatagttta
//
LOCUS       DACCKU010000088        11548 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.091, whole genome shotgun sequence.
ACCESSION   DACCKU010000088 DACCKU010000000
VERSION     DACCKU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.091"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(53..295)
                     /gene="acpP"
                     /locus_tag="IVN13_002303"
     CDS             complement(53..295)
                     /gene="acpP"
                     /locus_tag="IVN13_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP7012762.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(417..1418)
                     /gene="plsX"
                     /locus_tag="IVN13_002304"
     CDS             complement(417..1418)
                     /gene="plsX"
                     /locus_tag="IVN13_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP7012763.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(1467..3503)
                     /gene="recG"
                     /locus_tag="IVN13_002305"
     CDS             complement(1467..3503)
                     /gene="recG"
                     /locus_tag="IVN13_002305"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP7012764.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(3620..5302)
                     /locus_tag="IVN13_002306"
     CDS             complement(3620..5302)
                     /locus_tag="IVN13_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP7012765.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(5354..5716)
                     /locus_tag="IVN13_002307"
     CDS             complement(5354..5716)
                     /locus_tag="IVN13_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP7012766.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            5986..6174
                     /locus_tag="IVN13_002308"
     CDS             5986..6174
                     /locus_tag="IVN13_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP7012767.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            6412..9081
                     /locus_tag="IVN13_002309"
     CDS             6412..9081
                     /locus_tag="IVN13_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7012768.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(9172..9792)
                     /locus_tag="IVN13_002310"
     CDS             complement(9172..9792)
                     /locus_tag="IVN13_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP7012769.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            9905..10444
                     /locus_tag="IVN13_002311"
     CDS             9905..10444
                     /locus_tag="IVN13_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012770.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            10441..10779
                     /locus_tag="IVN13_002312"
     CDS             10441..10779
                     /locus_tag="IVN13_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HAP7012771.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
     gene            complement(11160..>11548)
                     /locus_tag="IVN13_002313"
     CDS             complement(11160..>11548)
                     /locus_tag="IVN13_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7012772.1"
                     /translation="NIIEELSRNSRITMKELGEKVHLTGPAVSARVTKLEESGVIENY
                     TVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGCYLLECRF
                     TSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         3296 a         2324 c         2039 g         3889 t
ORIGIN      
        1 aaggataaaa agctgtatgc agctttttat ccttctttgc ttttatttga aattatttaa
       61 gatggctgct gatataatct actgcgccac cgactgtttc aatttgttca gcgtcttcat
      121 ctgaaatttc cgtaccgaat tcatcttcaa gctctaaaac gaattccata atactaattg
      181 aatcagcttt taaatcatct ttgatgttca attcatccgt cactttatcg gtatctatct
      241 caaaatgatt tgaaataatt ttcgctactt tgttaaatac ttcttcgcga gtcaactttt
      301 tcacctccaa gaatcataca cttgtcaatt acctacacca tggctaattt tactggcatt
      361 tttcaggaat gtccaccttt ttttcaatgg aacggattgc ttcagatatc caaatactat
      421 tcttcgcttt tttcaaattg ttcgactaat tgtccaacta catctgtctc aagcattgtg
      481 tggatttggc ggatcgtata gcgaacagca tcggggcctg ttgctccatg agttttaatg
      541 actggtgctt tcaagccaaa caaaacggca ccgccatgtt tggaataatc catttctgct
      601 ttcaaagaac gtaagccgtc ttttaaaagc atagcgccca tttttccttt gatcccttca
      661 tttagaatag cagattttaa tagattcatc atattcattg ctgttccttc aatagatttc
      721 aaaacagcgt tgcctgtaaa tccatctgtg accactacat cagccacacc attgagtagc
      781 tcacgagctt ctacgtttcc aataaaattc aagcttgttt cattctgaag gagttcatag
      841 gcttttttcg tcacttcact accttttgtt gcttctgttc cgttgtttaa gagtgcgaca
      901 cgaggacgtc cgatacctcg gactttacgt gcataaaaag aacctaaaat accgtattgc
      961 aacaaatgtt ctggtttgtt ttctgcgtta gcgccaagat ccagcatatc gaaaccggca
     1021 ccttctttcc ctactactgg caaggtagac atcaagccag ggcgttcgat atttttgata
     1081 cgtcctacga tgaacaaccc tgctgctagt agcgcgcctg tattccctgc tgaaaataca
     1141 gcatctgctt caccgttttt cactgcttgt gcagcaagga ccattgatgc agtctttttt
     1201 cgtcgaattg cttttactgg ctcatcatca ctattgattt tttcgtctgt atggataatc
     1261 gtgatgtttt tttcatttgt taagtatttc ttgatttctg cttcttttcc gtaaagtaag
     1321 aattctactt ctggaaaatc ttgctgcgct aaagccacgc cttctacaat tgcttgaggt
     1381 gcgtgatctc cgcccattgc atctacggca attttcattg tttccactcc tttttccgtc
     1441 atcagtataa agtatagcac agtctgctaa tcgaaataac gattatcctg attttctatt
     1501 tggaggcttt gcgctaaagg ctgatactca ggaagcatct gccaatcctt tatctgccaa
     1561 agggctgcag cctcttctct ggctacctcc aaaacatttc catccgtcac caagtcagca
     1621 atcgcaaatt gaggcagacc agattgacgg aagccaaata cttcaccagg accacgtaat
     1681 tccagatctt tttcgcttag tacaaaacca ttggtggtct ctgtcatgat tttcatccgt
     1741 tccacaccta gttcattttt aggattagca actaagatac agtaggaagc atcacttccg
     1801 cgtccaacac gtccacgaag ttgatgcagc tgtgccaatc caaatcgatc cgcatccatg
     1861 atcagcatga cggtcgcgtt cggcacgttt acccctactt caattacagt agtcgaaact
     1921 aatatctgca tctggttgtc tttgaaggct tccatgatta cttctttttc ctgattcttc
     1981 attttaccat gtaacaagcc aacttcatat tttggtgcaa atagttcacg aagtttttca
     2041 tagatttcca ctgcattttt tacatctaat gcttctgatt cttcaatcag cggacagatg
     2101 acatacattt gatgtcctct tgccagttct tttttcgtcc aatccagtac actgtccaat
     2161 tgaggtgttc ttacccagcg tgtttcgatc gggatacgtc cagctggaag ttcatcaatg
     2221 atagatacat ccatctcacc atatgctgta atagctaatg ttctaggaat cggagtcgct
     2281 gtcataaata agacatctgg tcgccagcct ttttcacgaa gtactttccg ctggttcacg
     2341 ccaaaacggt gctgctcatc cgtaataact agacctaaat tctgaaattc gactccatct
     2401 tggatcaatg catgtgtgcc gatcacgatg ttaatttccc cttgctctag tttttcaagg
     2461 agctcccgac gttcttttgc tttcgttgac cccgtaagca atgcgatggt tacttcattg
     2521 ggatcaaaca gctgttgcaa gctttccata tgttgttgtg ccaagatttc tgtaggcacc
     2581 atcaacgcac cttggaaacc tgctgtaacg gtagcgtata acgcaatcgc ggcaaccact
     2641 gtttttccgc tacctacatc tccctgcaaa agtcgctgca tatgctgcgg actgcgaaga
     2701 tcccgacaga tttcattcgt tacacgtttt tgtgcatttg tcagttcaaa aggtagcttt
     2761 tgagtaaatt ctttcaattt ttggacatca taaaggatct ccaaaccatt cgtttctgct
     2821 ttttctgctt ttttcaatcc ttgaagacgc atttgaaata aaaagaactc ttcaaaaacg
     2881 acacgtcgtt tggcttgatg gttttcttcg tggtcttttg gaaaatgcat acttcttaca
     2941 gcagaagctc ggtcaagcag tcgatatttc tccacaaggt tgtttggcag attttcttca
     3001 atcaatgtgc cgtattcttg aaaccctttt ttgattagat caactaatgt cgtttgacga
     3061 attgatttgt tgacgtgata tatcggcgaa aagtcatcca ttgtttgaga acctaaaatc
     3121 ttcatcccgt tcaatgcttt acgctttgca tcccatttac cgtatacggc gatttcctcc
     3181 gataaaatga ctttttcttt tagataaggt tgattaaaaa aagaaacgtt gaacacatca
     3241 tgttcttgca tcatacgaaa ttgcaatcgg ctttttttat aaccaaatcg attcatcaca
     3301 ggaggcgaga ctacgattcc tttcaaagta accttttctt gatcttggat ctcgtttaga
     3361 tttctttcct gaatatcttc gtagcggaaa ggataataag tcagtaagtc ttcaatcgtt
     3421 tggatgccca agctggcaag actagctgct cttttttctc cgactcctgc cagcatagtg
     3481 attggatcat ctaaagaacg cataaatttt tcctccctcc tttaacaaaa aaaggacttg
     3541 gtcagcgatc atacctgatc actaaccgaa ggccttttat gaatgaatac tgtgttagaa
     3601 agatattctg acacagaaat tattcagcag aaaatagata aggatagaca ggctgatctc
     3661 cttggtggat ctcgatttcc aaatcaggat gtttttctgt tagtttctca gcaaattcat
     3721 ttgcttgttc aactgttcct tcatcacctg ccaagatcgt tacgatctct gtgtcctcat
     3781 tgatcatttg ttctagtgta cttaacgctg ttgctgtgat atctgggtta gagatcagga
     3841 tttttccatc gatcatgccc aaaaagtctc cttctttgat ctctacaccg tcaattgagg
     3901 tatctctgat cgcgtgagtg acttgaccgc tcacaacgct agacaacatt tctgtcatcg
     3961 tttctttgtt ttcttctaaa gaagcttgat cattgaatga aagcatagct gtcaatcctt
     4021 gggaaatcgt tcttgaagga acaactgcta ctgggatatc tgctacttct gctgcttgat
     4081 cagctgccat gaagatgttc ttattgtttg gcaagacaat cactttatct gcgtttactt
     4141 cttgaatcgc tttaacaatg tcttccgtac ttggattcat tgtttgccca ccactgataa
     4201 cataattagc gcctaaactt tcaaacagct cttggacgcc tttaccagca gcgatcgcta
     4261 cgataccata cgggatacgt ggtgtttctt ttttcgcaaa ctcgttcact tcttcatcgt
     4321 gttccaaaat cgtctcatgc tgcaagcgca tgttgtcgac tttgattttg actaatgaac
     4381 cgaatttttg tccataattc aagacttctc ctgggttttc tgtatgcacg tggactttga
     4441 tgatctcgtc atcattgacc acaagtaaag aatctcctat accatctaaa tagttacgga
     4501 attcttcgta atcgaatgta ctatcgactg taggtccttc tccgattttt accatgattt
     4561 ctgtacagta accaaaatgg atatcttctg tcgccatttg accttggaca ctgcgatgat
     4621 gttctgcatt gaccatatca tccatttctg ctggagaagg ttcatatgtt tcatccgctt
     4681 ggaattctcc attcaatgca cttaaaaagc cctcataaac aaacagcagt ccttgccctc
     4741 cactgtctac gacaccgact tcttttagga ctggaagcaa gtctggagtt ttagctaaag
     4801 cacgctttgc cccttttaca acagctgtca ttacttcaac gacatcatca gtactttttg
     4861 ctttcttttc tccagctttc gctgcttctc tagctactgt caaaatcgtt ccttctactg
     4921 gtttcattac tgctttatac gcagtttcaa ctccatgagt gaaggctgca gcaagatcgg
     4981 aagcatttaa tgagacaaca tctggaattt gcttagaaaa tccacgaaac aattgagaca
     5041 aaataacgcc ggaatttccg cgcgcaccca tcagcaatcc ttttgaaagc attgctgcta
     5101 attctcctac tttttctgag gttgaatcta ctactgcctt agctccacta gtcatagata
     5161 gattcatatt tgttcccgta tcgccatctg gcacagggaa tacatttaat gaattgacgt
     5221 attctgcatt tacttgcaga cgattcgcac ctgcctggac catttcctgg aactggcttg
     5281 cacttatttc tgttacactc acctagataa tcctccttca gatcgactgc tgagttacca
     5341 gccagtctcc tgcctagtct ggcagtacac gaacaccttg cacaaagaca ttaactgaat
     5401 tggcagttac gcctaataat gcctctagat tgtatttcac tttttcttgg acgttgcggg
     5461 aaacttcaga gattttcgta ccatagctga taatcgtata aacatctaca gcaataccat
     5521 tatcttcttg gcggacaact actcctcttg agtaattttc tttacgcaaa atctcagtaa
     5581 tattatcttt aatttgattt ttgctggcca ttcctacaac accgaacaca tctgtcgcgg
     5641 cgccaccaac aactgttgca attacatcat tggtaatttc aatggttcca gctgacgttt
     5701 gaatttttac agccatgaat gagcctcctt aacgggtaaa aaactcccat ttttcttcta
     5761 tcatatttta tcatagcgca acttgaaata aaaggcagtt acccaaagta ctcaaaaacc
     5821 ttgcaaaatt tcaggtataa ccttgtttaa tttacaatta ttcttctcaa ttgtcaattg
     5881 tatctatgaa aatgtacttg caaatttcca ttctatatga tatgttagtc tggtatgagc
     5941 tgaattattc tattgctcca aatcaaggca aaggaggaaa ttaccatggc aaaagtttgt
     6001 tactttacag gtcgcaagac aaaaagtgga aacaaccgtt cacacgctat gaactcaaca
     6061 aaacgtactg ttaaacctaa cttacaaaaa gttcgcgtta tggtcgacgg aaaacctaaa
     6121 aaagtttggg tgtcaactcg tgctttgaaa tcaggaaaag ttgaacgcgt ttaattgaat
     6181 gatacttgga ccgttcagat agtgaacggt tttttattta ataaaaattt ttaagggtgg
     6241 gacttttgat tcctaccctt tttttgcgtt tatttttgtt atacgaacaa attatgaagt
     6301 tatttattct aaattttatt tttttgtgaa aactagactc tttttaagca gagaaaaaca
     6361 aggatatgat aaactgaata caacttcata gaaatttggt ggaaaataaa tatgaaaaaa
     6421 aatttaaagt cattctttag agaaaacgga cgctacgcag cagctagttt cctgattccg
     6481 tttttgattt tagcacttgt ttatctaagt atcgggatct atcctggaag cagcagaagt
     6541 attttggcaa gtgatgcctt ttcacagttt tctaattttc atgccagctt ccgaaatgtt
     6601 cttcttggaa agcaaagtat tttttataca tggaatgctt ctctaggatt gaattattta
     6661 tctttaattt cttactatct aggcgggatc ttcacgccac tggtgttctt ttttccaaac
     6721 cagatgatgc cagatgcact gtatgttctg actttgctta aggtcggctg tgccggattg
     6781 gctttttggt ttcttgcacg tacgttcaag attcctcgct gggggcatgt tgcacttagc
     6841 gtcagttacg cattgatctc atttatcact gcacattcgg agatcatcat gtggctggat
     6901 gcatttgttt atcttccatt agtgattttg gggattcatc gtgttatgga tcttaaacgg
     6961 ccgaaacttt tatttatcag ttatttactc ttgtttttat ccagttttta tatgggtttt
     7021 atgattggcg tattttcttt cttatatttt atcgcccgtc tattgacaaa ctggtcagaa
     7081 tataaaaaaa cgattgttcc ttatggtatc acttctttac tggcaggcgg cgcttcaatg
     7141 atcattgtat tgccagcagt gctggatctt cggtcaaatg gcgaggcact gagtcagatc
     7201 actacgttga aaacagaagc gacttcttat ctggatctaa tcatgaaaaa tatgattggt
     7261 gtttatgaca cgacaaaata cggatcgatt ccgttcgttt acgcaggtct gctaccatta
     7321 gtcctctgtc tgttttattt tgccagtcgg aaagtaccgt tgaaaaataa atttttgtac
     7381 ggcagtcttt tcgccatact tatcgcaagt ttctatcttg ttccattgaa tttgttctgg
     7441 catggtatgc atgcaccgaa tatgttttta ttccgttatg cctatctgtt ctctttctta
     7501 gtgctcctgc ttgcaggaaa aggatgggaa aagatagaac aagacgatcg tgggctgttg
     7561 ttaggaatca ttttggtcgt agctgctctc tttgcggtgg catggggcat caaacctgaa
     7621 ggaagctaca cctatgtgac gatgacttcc tttgtattga cagtggtttt cttagggatt
     7681 tatgcttttg ctgtgggatt ttatcagcat aaaaaacttt cattcaatca tctgagtata
     7741 ttgttattat tgatcatgtc tgctgaagca ctggtcaata cgaacagtat gatccatggc
     7801 attcttgatg attggaatta tgcttcccgc agtttatata ctgatcctta tcctgacttg
     7861 aaagctcttg taaatgaaac aaaaaaagat tccgactcgt tttaccgctt ggaaaatctg
     7921 gatccagtat ctgcaaacga ttcgatcaac tatggttaca gtggtatcag tttgttttct
     7981 tctataagaa atcgtaattc ttcctcttac ctggataaat taggtttccg ttcaagaggg
     8041 acaaatttga atatccgtta taacaataat actttgctaa tggatgggtt tacaggaatc
     8101 aaatataaca tttctaaaaa cgaaagcacg ttcagtaaat atggcttccg ccaagctggt
     8161 caaagtggcg actatacact gtatcagaat gtcaatgcat tgccactagc atttactgca
     8221 ccgttgtccg ttaatgatgt tgagcagcca gtgacagata atttgaccag tcagacaaac
     8281 ttgatcaata cattgtctgg tttaaatgaa cagtatttta cattctatac acctactttg
     8341 aagaatcagt tgaatacgac gatcacaaag acaacgactg gcgtcactta ctcagaaaca
     8401 gcagataacc agccaaaaga gctcacttgg gaagtccatg tgcctgcaaa tacacaagct
     8461 tacttaagtt tgttcccaac caattttgca caattagaaa gttctagtgc gacgatcact
     8521 gtcaatggta catcgcgaaa aacgcaaatc aacatttctg gtcaatacta tgatcttggc
     8581 tattatcctc aagagacaac agtgacattt actgcaagtt tttatggaac gaaagaagta
     8641 agtttcatgg agcctaaagt cgttggtcta gatgttgtag cctatcaagc cgcaatgaat
     8701 cagatcaaag aaaaaggtgt cgaaatggaa gccactggca gaaaagcttc tgggactgta
     8761 aataccgctg aagattccat gttggtgaca actattcctt atgacaaagg atggaaagct
     8821 aaaattgatg ggaaaccagt caaggtcagc aatttcaaag acgcattctt gatgataaaa
     8881 gttccagcag gtaaacatac tgtcactttc agttacttgc ctgaaggatt tacgataggt
     8941 gcaatccttt ttgtcctatg tattgcattg tttattcttt atgtctggta ccttgaccgc
     9001 aatcgcccct tttatggcac gattgatcca gcaatagaag aaaaaccgcg aaatacacac
     9061 agaagaagac gaagaaaata aagaaacgat ggaatctgtg tcactcttct atttaaatgg
     9121 caaaaatttc aatctttaaa tgacaaaaac cacctgattt ttggtggttt tttatatacc
     9181 tatgactaaa ctatctcttg ctgcttcgac aacttctaca gtgatcttat ccatttgatt
     9241 gattcttaaa atccgatcaa tctgcgcaaa taaacgatga ataagcctaa aatttccttt
     9301 tgtgatctta atgatagttg tgatagcctc ataatcagaa aaatcttcta gcttgatatc
     9361 aattcctaaa tctgcccatt tgtattccaa aatatgatgt gtttcgtcct tactaaggtt
     9421 atcaaattca tgtgcaaatc ctatgcgaga gtaaagctgc ggataacgag acaatctttt
     9481 ttcaatacca ggcataccga taagaaccat tgccagatca tttctatcgt aaatatctct
     9541 tagctgttct aaatgctgaa gctttaaacg atcaatttca tcgacaataa ttaagtcaat
     9601 tgcttcatag gttttaggca caggaggatc ttcttgattg tattttagta ccatatgcat
     9661 tgctttagtc agacctattc tactagttat tgtgttaata tccgaactga cacgagacat
     9721 cttttcagct ggtacagtat aaaaaacttt atttacactt aatatttcat ctgttgcatt
     9781 tatcaagaac aagaaaaagt ctgaatattc aaaaaaataa cggtgtacat ttttaaaagg
     9841 agatgtataa tcgagattga gaaagcgttt tcctcaaaat ttaatgaatt aggaagtgat
     9901 tactatgaaa aaattattta gcgcactttt tattctgtta tttggtttta ttggtattgg
     9961 gacttctgga aatatagtta aagctagcac gatggttgaa caaacaagtg aaatggcaaa
    10021 aacttttagt caattaacac cagaagaaca aagttacttc aaatccaaag gatttagtga
    10081 taaagatact ttttacactc aaaagataga gcctcaagat ccgaaaacac gtttttctgc
    10141 aaatgtattg cggttaaccg catctactaa aaaaattact aatacgcagg ggcggacaga
    10201 atatattata acaagtccca attcggcgat gtatcgtatt tctacacgct tgagtttagg
    10261 tagtgcaaaa tcatatactc aaacagtaaa tctagctaat tcttatggtt atggtggagg
    10321 aatgtatttt acatttactg ggaaaagaca atattacagt gtaaaattag ctatgcaagt
    10381 ttataataca tttggtgtag ggtcagtctc ttgcactgcg ggaggattga caataggaaa
    10441 atgataattt cttactatgt cttaaataga agtgatgatg aaggactctt cgtttttcaa
    10501 tcattacatg aggatacgat gctaaaagag ttttataatc ggattaagaa taaacagata
    10561 gaaacaaatt tatatttttt aagtgactct atcttgcctt ttattagcaa ccctaaaatt
    10621 cttaacttgt tgcagacaaa agatttgaaa agtttaccga ttattgtcat tgatgaggaa
    10681 attgtaaaat atggcgctta tctaagcgta gaagaattag aaaaattaac taacattgga
    10741 atgagtattc aggaggataa tggggtacat ggaacataga atgaatatgc acagttgaga
    10801 acggaatttg acatttattt agaccaacag ggtataccct gttggtcttt ttgtgcgttc
    10861 atgatatact tggtgtcatt tttatattta aatggcacaa atatttctaa ttatttgtca
    10921 tttaacgctg aaaatttttg ctatttatct aaaaaagtga caggatactc atttggcctt
    10981 catagaattt tctaaatatg aatcatcaat gaatttaatt aaaaaaataa gtagggcaaa
    11041 atataaatgt ttttgcctta cttgtttcca tcagtttagt tttaaattga taagttatat
    11101 agttatggtt gaatgtgtgt ctcgcatatt agtattttta ccatctgcta gttttattat
    11161 taatctataa caatagataa tttataattc gcatattcat taagctgttc caaaaagcta
    11221 ttcattgatt tttttgaagt aaatctacat tccaaaagat aacagccccc tccactaatc
    11281 ttataatttc taatcaagaa tttttcatga tttttaataa atgataaata gggtttatga
    11341 tttaaatttt gcgtaattat aattaaaaaa gcgtgaatcg aaaaccctag cttttccata
    11401 tttaatttta ctgtataatt ttcaataact ccactttcct ctagttttgt cactctcgcc
    11461 gagacagcgg gtccagtcaa atgtactttt tctccaagtt ctttcattgt gattcgacta
    11521 tttcttgata gttcctcaat gatattta
//
LOCUS       DACCKU010000089        11462 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.092, whole genome shotgun sequence.
ACCESSION   DACCKU010000089 DACCKU010000000
VERSION     DACCKU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.092"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            131..3658
                     /locus_tag="IVN13_002314"
     CDS             131..3658
                     /locus_tag="IVN13_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP7012773.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IVN13_002315"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IVN13_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP7012774.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IVN13_002316"
     CDS             7446..7787
                     /locus_tag="IVN13_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7012775.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IVN13_002317"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IVN13_002317"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP7012776.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..>11462
                     /locus_tag="IVN13_002318"
     CDS             9144..>11462
                     /locus_tag="IVN13_002318"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP7012777.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLT"
BASE COUNT         3938 a         1962 c         2467 g         3095 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 ca
//
LOCUS       DACCKU010000090        11341 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.093, whole genome shotgun sequence.
ACCESSION   DACCKU010000090 DACCKU010000000
VERSION     DACCKU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.093"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            719..2635
                     /gene="ltrA"
                     /locus_tag="IVN13_002319"
     CDS             719..2635
                     /gene="ltrA"
                     /locus_tag="IVN13_002319"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7012778.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            2752..3552
                     /locus_tag="IVN13_002320"
                     /pseudo
     CDS             2752..3552
                     /locus_tag="IVN13_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            3549..5927
                     /locus_tag="IVN13_002321"
     CDS             3549..5927
                     /locus_tag="IVN13_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012779.1"
                     /translation="MIIGGIFSGIADWWSGLFGDGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKIKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLENDPNKVEHIPTDKRNAFTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQVNA
                     AVKANTTTEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKVGLPKAENLPTK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLSESGETENASVQSIV
                     TREAAPKPIQNVHTGYKPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKS"
     gene            5979..6311
                     /locus_tag="IVN13_002322"
     CDS             5979..6311
                     /locus_tag="IVN13_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012780.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            6312..6905
                     /locus_tag="IVN13_002323"
     CDS             6312..6905
                     /locus_tag="IVN13_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012781.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLVTKE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            7068..9092
                     /locus_tag="IVN13_002324"
     CDS             7068..9092
                     /locus_tag="IVN13_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7012782.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYSGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            9092..10297
                     /locus_tag="IVN13_002325"
     CDS             9092..10297
                     /locus_tag="IVN13_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7012783.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTEHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            10313..10957
                     /locus_tag="IVN13_002326"
     CDS             10313..10957
                     /locus_tag="IVN13_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012784.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            10968..>11341
                     /locus_tag="IVN13_002327"
     CDS             10968..>11341
                     /locus_tag="IVN13_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012785.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLS"
BASE COUNT         4093 a         1829 c         2383 g         3036 t
ORIGIN      
        1 aatagtgtga cctgtttagc ctaaggataa aagaggctag ttccaacgaa cattggaata
       61 actttactat cgagaaatca aatgacgggg taacgccctg aagggtttcc actgatactg
      121 cgacatgcgt tagtaggatg tgcatccaaa cgcgtatgaa gctcgctgaa gtcggcagaa
      181 gggtaccgta aggaaagaca cgatacgtat ctaccacgca agtgctctag aggtagggta
      241 tgtacctgat atgtcgggtg tcctaaaaag ttcaataagg tgagaatgat ttgattatct
      301 gacaaacttg cgaatggaat gtctgaaacg aataactcat agaaatgtga gggggaaggg
      361 ggatacgtcc tctttccatt gactcgaggt tgagtaaaaa tgtgtttcta tgaaaaacct
      421 tgcatggtta aaggctatgg agtagtcaac aggcaaaagc aagcacctaa gttgaaatct
      481 atggatagat agtaaggaac aggggaagca tctagcacgg agggagcata ccgaagatgt
      541 gtttctaagg aaaaaccgaa atcggttata acttaggtga atagatgtga aagtagagcc
      601 acgaccagtg aaaatttcgt aatgaaattg gaggaacagg ctcaagtcgt tatttataat
      661 ataagaaacc tatttaaaat catggattct cgtaagacta aaaaggtcac gaaccagaat
      721 ggagcgtgat aaaaccatga ctaacacaaa aggaattagc ttaaagaaca aacaactcag
      781 acacgccgaa tactacgaca tgactgagac tttcgatgaa ttgcacgcaa tgagtgttgc
      841 aaaatcaaat tttaaaaatc tcatgaaatt cattattgat gacaataata tctttctagc
      901 gtatagaaat atcaaaaaga ataaaggaag tgcaacggcc ggatacgatg gtgtcactat
      961 aaaagacatt gaaaaattgg catgtggtga ctttttaact attattaaaa ggcgatttaa
     1021 ggattacaga ccaagaaaag ttaaaagagt agagatcccg aaaccaaacg gaaaacttcg
     1081 acctttagga attccttcaa tatgggacag gatagtccag caatgtattc tacaaatttt
     1141 agaaccaata tgcgaagcaa aatttaataa gcatagctat ggattcagac caaattgttc
     1201 tacagaaaac gccatagcag attgtcttgc tagaatcaat caaagtaaga tgaattatgt
     1261 tgtagatatt gatataaaag gattctttga cgaagtaaac cataccaaac ttatgcgtca
     1321 aatatggaca atggggattc gagataaaca actccttgta attattcgta agatgttaaa
     1381 ggctccgatt gttatgcgaa atggcaatgt tgtttttcca accaagggta ctccacaagg
     1441 agggatttta agtccactgc ttgcgaatat atgcctgaat gaatttgatt ggtggatagc
     1501 taatcaatgg gaggaacgaa ctgtcaaaga gctgaaacac cactatcgaa aaaacggagc
     1561 atgggataaa ggaaatatct attcgaaatt aagaagccac acaagtttaa aagagatgta
     1621 tctggtacgc tatgcagatg attttaaaat tttctgccga acaggagaag aagcccaaag
     1681 ggctttttat gccacacaaa aatggctcag agaaagatta aagctcccaa tttcaatgga
     1741 gaaatctaaa gttacgaacc taaataaagc tgaaagtgaa ttcttgggtt tttcaataag
     1801 actccacaaa aagggaaaaa agaaagtggc tttcacgtac gtttgtcgca aagcagaaga
     1861 aaacattaaa aataaactaa aacaacagat taaaaaaatt caatacgctg gagcaaaaaa
     1921 aaataggcac cgagcaatag gtgactacaa catgatggtc atcggtatcc acaattacta
     1981 tcggatagct tcacattgta gtgtcaattt cactgagatt tatagaagtg ttaggacagt
     2041 aatagaagca agactgaaag ctagaggaat gacgaaaaat ggaatctatg gacaagatca
     2101 tgaaggatac cgtccgtatc taaagtctaa gcaaatgaga tatattgaag gttttccgat
     2161 acttccaatt ggctatatta aaaaaagaac tccactcaac aagcggagaa atctaaataa
     2221 atataccaag gaagggagaa agctcattca tcaagagcaa caagctatag aaagctggaa
     2281 aatcaagtat cttgtaaaag gttttagtaa caataagaaa tacacggtgg aattttctga
     2341 taacaaaata tctaagttta tttcccaaaa agggaaatgc gctatcacag gaaacgaact
     2401 tttgatggaa gaaattgaat gccatcacat taacccatat catttgtcca aagacgatag
     2461 ttacaagaac cttgtcatta tctctagaga aatccataga ttaatccatg caactagtga
     2521 agaaactatt ctgaaactgc ttcggttact gaaactaaat cactatcaaa ttagaaaact
     2581 aaacacgtat cgaaaaaaag cagctaattt tccaatatca atcaaaatga attagatagg
     2641 aaacaaatta tattgtaatt aacgatggaa cgccgtgtgc ggtgaaagtc gcacgcacgg
     2701 tgtggaccgg gggaaaagac ggagataata tcaaactctt acctatcgga attgccacca
     2761 atggataata tggaaggaat catgacagtc acagctggta gaaacatgtt gtgggatttg
     2821 tttatccaat cctatcagca actttatgcg aagtatgggg aacaaaacgc agcgattatc
     2881 aaagaaaatt gtcagacaca tatttatatt atgtcgacag acgataatac catagaagaa
     2941 ttttccaaaa aagtggggaa taagacggtt gaacaagaga acagtaccat caattcatta
     3001 ggtatgaata ctcatatcaa tcgcaatgtt gattcagatc gcatcttaac tcctgaaaga
     3061 atttctactt ttttagaagg tgaaacgatt gtactcgctc ctttaaaacg acaagatttg
     3121 aaaggtcata aaatacggcc gtatcctatt tttaatcatg gggaaacgaa tcttccttat
     3181 gcttatcaat atattgaaga tttcaatcca tcgtgggata tcaatgatat agacatacat
     3241 tgtacacatg cggacttgga tcttaaggct ttacaaattg attacgatta ttttctacct
     3301 gatatctatg cacgtcgact ttatcacaat atgcacgatc aaatagaaga ggaagctaaa
     3361 gaaaacattt ttattaaaat cgttcaatct ttagacaact caacggcaca aaagttactt
     3421 attcaatttt atgaagagaa gcaaatagat cgtttgcaag aataccttga aaaattgcgt
     3481 caaaatgaag aaattacaga ggttataagg aaacgacttt tgctagaagc tgaaaaggcg
     3541 gtgaaagaat gatcattgga ggaatatttt ccggcattgc cgactggtgg tcaggcttat
     3601 ttggggatgg ggatgcagat cccaaaaccg taacgaaagt cttacaagat tatggtaatt
     3661 atctgcagta tcaagatgta ttcagttgga tttgtaatac ttttttatgg ggtttgatta
     3721 aagggttata taatctcaat actttactag aaaaaacaat ttatcaaagc tttgatttaa
     3781 aagacttatt gaacgccgct ggtgttaatg aactgtttca aagtttgatt agtaaggtat
     3841 tggctctttt ttgtgtcgtc acattaattt atttaggctt aaaatttttt atgagtaaac
     3901 atccaccaaa aatcaaaaat atcttggtta atctatttat ggccatgatt ttgatttttg
     3961 gtggtagttc tttaatcgat gaagggctaa atattagtaa aaacttttac ggagacgtga
     4021 ctgcagcaaa taaagcagat aaaactggtt cacctgcttt tcaaattatt caaaataatg
     4081 tgattgatgt gagtacgtta ctagaaaatg atccgaacaa agtagaacac ataccaacag
     4141 acaaaagaaa tgctttcacg gcgaaaaatt ttaaaacagc gaatatcaat gggattatta
     4201 cacctgatca agctaagaaa atgtctgaat caagtgatat tagtacagta caaaaagatc
     4261 ggttaaaggc attacagtat cgactagagt tagacgataa tgggaaagaa atccctgttc
     4321 aaattagtga tgaaggttta gcaaaatatg tgtatacttc aggctatcgc cgatacatta
     4381 gtagtccagg tattatttta gctggggaat cttctttgtc ggtagcctat ttatttatct
     4441 tgtttacgat tgttacttgc attattgagc tggtatttaa aaaattctat ttagtgattg
     4501 ccgcgtcaac ggactttgag acaggacaac ggatgaaaag tgctatagaa gatatttctc
     4561 aatccttctt gttattggca tttacgattt tagaattacg aatctataca ttaatgcttt
     4621 caggtatcgg tgatttgcat gcggcaggaa agatcaatgg attcttgtat gttgtcgctt
     4681 taattgtatt aaccattgcg ctattcaaag gctctcaatc agttacgaaa attttcggcg
     4741 ttgatacgag tctgaaaaac ggcagcaatt ctcttcttag catgtttgcc ttaggaaata
     4801 ttgcgaaaaa tgtgggtagt ggggctaaaa accttgcaag tgctggtaag agtggtttaa
     4861 gccatttaaa tgatattcgt aaaaatggat ttggccatcg aaaagatgtt acccctgata
     4921 acggggaaga acaatcagac gataaagcta caactgtacc taatagtaag aaaacagttt
     4981 ccgaaatgtt cgggaaagcg aaaaatggtg tttctaaagc cgcagaaggt cttggttacg
     5041 tcaatgaacg tgggttaaaa ggaacagctg aagatcttac agaaaaagcc aaagacacct
     5101 tagaagagaa attagacggc acgagagcta aagaattggc tgaagctgtg aagaatccat
     5161 ccgaagcact agaaaaacaa aaagaaaaag cttcagaagt caaagaagga ctaaaagatg
     5221 cgtatggtga aggacaagtc aatgcagcag taaaagctaa tacaacaacc gaaaaagaaa
     5281 atcaagaagg tgcagcgatt ccttcagcca aagatcacca agatgagtta cattctaacg
     5341 aaaattcgat cggagaaaaa gattcagaag aaggacaaca tggtcagtta ccaaaaaccg
     5401 atccagaaca atatcgttat aaatctaaca ttaattctga aaacgaaaaa cgcatttcag
     5461 atttaccaaa acgaaatcaa gaagtacaaa cggatgagga ttccatcgat ctaccaaatg
     5521 ataatggaat cgaagttgga actgaaaaat caacgaaagt tggtttgcct aaggcagaaa
     5581 acttaccaac gaaagatagt cgtttgaatc aattcaaaga gaatgatcaa agctttgttc
     5641 atcaatcacc tgcaggtact gttcaaatga aatcaagtcg tttgcctgaa aatgggaatt
     5701 tgacaactgt ttcttacaat ttatctgaaa gtggggagac agagaatgcg tccgttcaaa
     5761 gtattgtgac aagagaagct gcaccaaaac ctattcaaaa cgttcataca ggttacaagc
     5821 cagcaaatgt ggcatttact gcaccaacaa aaataaatgt aacaagagaa actaaaaaca
     5881 ggaacaatga attgaaagaa caattattga aggatatcaa atcataaaag gcctacgagt
     5941 taggcctttt ttctatgtat gaaaaggaga aatcatttat ggataaatat attaacatga
     6001 aaaatcctcc aagatttagt ggggaatgga agtttttcgc ctttttcttt ttagatctat
     6061 taacgcttgg tggttgcttt ttttgggcga aaatagtcaa taatcttgtc cacttaccac
     6121 tcttttttgc actcttgaat tatggcttat ggattgcgat agctctagtg attgtttggc
     6181 gaccctataa taacccaggt caacgacaaa tttttgcgat tatcgaatct attttcttta
     6241 cggataagaa ccactatcat gcgttagatc ctaaccgaaa ttatcatggt acgtataagg
     6301 aggatgaata aatgcgagtc aaaggacaaa attcagttga taagaaatcc attttgcagt
     6361 ttggaaaacc agaagaacga tctatcttgg atttatttcc ttataaatcc tgtacagaag
     6421 agggagtctt agttactaaa gaagatcatt ttcaacgtta ttatcgtgta gtttctacag
     6481 acgtggaagg attaaacgaa caagagaaac tagaacgaat gaatcagttg accaccgtca
     6541 tgcgtacgta tgtgccaaca attaagttag ttagtttgac cacagagacg gatctaacgg
     6601 aacaaatcgt gcaaaagcgg caattattag ataagaatcg tatggaacaa tccttaggaa
     6661 gaaatctaca gcgattaaga aaatatgaaa agaaattagt agaggaaatc caggaattga
     6721 aacgagcaga acaagaacgt cctgacttaa gcttcttttt cgtcattgaa ggaaagacaa
     6781 tcaaggattt acaaaccaga aatcgtcagt taatgcgatc tagtggcatt ttagggttaa
     6841 aaccactcta caaaaaagaa ttgatcaaga ttctttatcg aatgaacaat atgaatgatg
     6901 agtaggaatg gagaagagag aatgaaatat ccgttgatat ggttagaaaa tgatgttccg
     6961 aaggaagaga tcactagaaa atttcctcac actttaagcc tgggtaagtt gggaaaaggg
     7021 caatctttta accaagtaaa gaatcaaaca agaagaggtg actgtacatg ttagaagcga
     7081 ttaaaacagc tttttcacca caaaatgatc aaaaagtact tgatccatta aagcaactag
     7141 gaaatcggga aatagaaatt ttaaaatcca aagggtatga tttagatttt ttaaaacaag
     7201 tgatgccaca aggtggatta aatttcgatg aagagtatgt gacctatgga aatggttatt
     7261 cacgatgtgt gacagtctat cactacacaa aaaatcctac tcttttttgg ttggccaatc
     7321 tcatgaacaa tcctcataca gtggccacac tagatgttag cacagatgaa aagcaaaaag
     7381 tgattgatca aatcaaccgt gcattagatg aattaaatga tcgatcagat aatgaacgaa
     7441 aagcgactga ccgaaataaa agtgcggaag aatacctgga actagcggaa tatgcccgaa
     7501 aattgacttc taatggggaa atttctaaac aagtcaaagt gagagtacat gtttacggaa
     7561 acacgattca agaagtagat gaacgagcgc aggaactgat cgaaaatctc aaaggaatgg
     7621 attatcgtgc ggtgacttat ctgcataaaa ccaaacgaga ttggcaatcg atgtttacca
     7681 tgttcgatga acaagctgaa tggttcggaa atatcaaagg gcaatcacta ccatcgttaa
     7741 acattggcgg aggtttcccg ttccaccacc aaacgctaaa agatccagga ggtacacaat
     7801 atggggaaac catgacaggc ggcccatttg tctggaatgc gacgtataaa aatagtgtac
     7861 ggttgtctac taataccttg cttctaggaa tgatgggagc tggtaaatcg accgttctta
     7921 aaatgatcgc ggaagcccat ttagctgcag gagactttat ttggggcttt gaaaagggaa
     7981 aagactttat tcctttctta aaggaatata gcggtattat ggtgcgttta gacggatctg
     8041 acggaatgat caatccttta gagatatttg ccacacggac gtatgatgaa gcatccagtt
     8101 tatatgacga tggatcagtc aaagatttga agatcaatga agcagcgtcc tatcaaacgc
     8161 acttagacaa agtggtctat caggtacagt tagtgagtcc tcagctaaaa ggaacgatga
     8221 aagcagaatt taagacctac ttaaatcgtt tttatgaaga gtatggaaca gtaccacgcg
     8281 gctttacttc ctcaaattcg cgttcaaata ctgagacgca agtcacaggt aaagatccag
     8341 aagcttatcc gacgttcaaa gaatttttag actttttagg acaactggaa ttaccaggtg
     8401 cttcccaaga gaaaaagaat cgaaaagaag aaatggaatc catcgtagaa tccttatgtg
     8461 agacgtatgg catgattttt gatggtcata gtacgattcg acaccttgac caacaacagt
     8521 tggtcttttt tgacattgac tctattacag gcttagatcg aggggtacaa caatgtcaaa
     8581 tgtacatggc tatgacgttg atttggaatc aggcattgat tcaaggaaga agacaacgac
     8641 gtttaattaa agaaaaacaa atggaagaac aagacttcaa acgcttcttg gcgatgattg
     8701 atgagtgcca taatgcgatt aatccagatt ttattgaaac ggtaaattat gtcacaaact
     8761 ttcaacgaga aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag
     8821 aaatggtgcc agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat
     8881 tttcttctac gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat
     8941 tagttggtaa atcaatgaca aactcagaat tagaatccat tcctcaacaa aatcaaggag
     9001 acgctgtgat caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac
     9061 gtcaattaaa attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa
     9121 gcagtatgtt aagcgtcgtg caaagcgacg cttatttttg tggttgtttg gtacttctgc
     9181 aggtttgatt actattttga ttactgtgtt cgtgaccctt tttctaatac ttgcggcggg
     9241 ttcgattgat aattctgatt cggattcaag tagtggtggc gaagctttta caggagaata
     9301 ttcggaagga ttacccattt ataaagaaat caaaggaaga ggcccttttt ctgatgagat
     9361 tgcgcaatat gctgtaggtg cagcagtcaa atataaatta ttaccaagtg tgatcctttc
     9421 gcaatacggg tatgaatcag cgtttggtac gtcggcgtct gcaagaaatg atctcaatta
     9481 ttttgggatt acttggttcg atggctgttt atttcccaaa ggaactgcgc gtggaattgg
     9541 tggcattgaa ggtggctggt acatgaagtt tcccaactca aaagctgcat tttcctatta
     9601 cggatttatg gtagccacac aatccaattt taatgcttgt gtcggaaata aaagccctgg
     9661 tgcaagctta ttgatcttag gacgaggcgg ttatgccgct gcaggaatta cggaagatag
     9721 tccgtattat acaggttgta tgtcaattat cacttctaat aaattaaccg aatatgatga
     9781 attcgccatc aaacattggg gtgaaggtgg caataacaac ggcacgatca ctggtgaatg
     9841 gacgaatcca ttccctggca gcagtttaga taaaagttcc tttagcggtg gtcaattatt
     9901 tggaacgaat ccagggggag agttccgacc gaatggtttc catgacggat tagattttgg
     9961 ttcagtcgat catccaggaa gtgaaattca cgcggtgcat ggaggaaaag tcgtgtacgt
    10021 tggcaatcct ggtatttcag gtttaggtgc atgtgtgatc gtgattaatt acgacggttt
    10081 gaatatggtg tatcaagaat ttgcgaatag tacaggaaat tctcgtgtga aggttggaga
    10141 tcaagtgaaa gttggtcaag taatcgggat acgagataca gaacacttgc atttaggttt
    10201 cactcgaatg gattggcgtc aagcacaagg acacgcattc acagatgatg gtacatggat
    10261 cgatccatta ccatttttga atagcagcaa aaaatagatc aggagaaaaa atatggaaaa
    10321 taaaacttta aaaatcgtct taacagctgc aattagcgtt agtttaatcg gcaatatcgc
    10381 gttgttttgg cagcttaaag aagaacaaga gacaaatacc tttaaaattg aagaagtggc
    10441 taaaaacgcg tctgagaagc tcaaaaaaaa ttcctctgag gcacaggcga agatcgacaa
    10501 gttaaatcaa accatcaatg agaaaaacca agaaatcgat cagctgaagc aacaagatgg
    10561 cacaaaagca aaagaggaag tcactgaagc acaaagaaag acggcagaag agttcgggca
    10621 attggccatt gataaatcat taagcagaaa tgaattaacg gaaaaactaa aagatgtcgc
    10681 aacccaagaa gtgatcgaca aattaagtcc tgcagacgat gatcaccaac atgatgatta
    10741 cggaagttac tcctttacat taggtgacgt gcaaacttac gctcaaacaa gcagtgtaaa
    10801 agaggaacaa aactttgtgg tatttgttga ttatacaatt ggcaatcctc aattcaaaga
    10861 tgtcaaacca caaaaaataa aaggtggatt aactgtcact gaaaaacagg tagatggtca
    10921 atggaaagtc acagacttta gttatttcac tcgttaggag gaagaaaatg agtaagaaaa
    10981 aaggagcaag acaacgtttt aaaaaacgaa aaagttatct tagatggctg ggaatcttta
    11041 tgattctcgg ctttttgatt cttgctagtc cgatttacac gcataagaag gtagacatac
    11101 cagatgaagt aagcacgaac gcctttttaa acacgaagga actgacgatg gtttcaaaag
    11161 aaaaaggagc gaaccagcaa ttagtttttg aatttgcgat tgacgatgaa gatcaaagct
    11221 tattaaaaga actggctaat cttaattatc gcgtagaagt aaagacagct aaaggggatt
    11281 atcaagcaat aaaagccaaa gtgattaagg tgtctgatga ttattttgca gtgaagctat
    11341 c
//
LOCUS       DACCKU010000091        11214 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.094, whole genome shotgun sequence.
ACCESSION   DACCKU010000091 DACCKU010000000
VERSION     DACCKU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 11214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.094"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1..345)
                     /locus_tag="IVN13_002328"
                     /pseudo
     CDS             complement(1..345)
                     /locus_tag="IVN13_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1022..1095)
                     /locus_tag="IVN13_002329"
     tRNA            complement(1022..1095)
                     /locus_tag="IVN13_002329"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1059..1061),aa:Ile,seq:cat)
     gene            complement(1285..2304)
                     /locus_tag="IVN13_002330"
     CDS             complement(1285..2304)
                     /locus_tag="IVN13_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7012786.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGNVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYTFIMDYNTGKELARVRADGIY
                     RPDVNQGYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2315..2512)
                     /locus_tag="IVN13_002331"
     CDS             complement(2315..2512)
                     /locus_tag="IVN13_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7012787.1"
                     /translation="MILPDKYYQVIKWTVLTVLPATSVLVATLGKAYGWNGTDMAVLT
                     INAAATFLGVITGVSAYNLKK"
     gene            complement(2528..2770)
                     /locus_tag="IVN13_002332"
     CDS             complement(2528..2770)
                     /locus_tag="IVN13_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7012788.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYESLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGITIIGYFLTKL"
     gene            complement(2827..4938)
                     /locus_tag="IVN13_002333"
     CDS             complement(2827..4938)
                     /locus_tag="IVN13_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012789.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSITSEEVTFSLDGTFMSEAGYYKHCYVEYRKGNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSGNPNLFVNKLESSDF
                     YMGYHGSLTNVNGMLHFMSDGTGDIDMFTRINTPQLASGKTYTLSAKVRFDEGTTGAI
                     NKLRLVYRTSPGENILLEANNTTITIDDVGKEITIKGTANVNYQITNLDRFYISVSFS
                     NRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANKGTTFPIKSSA
                     YEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPVEGLTDVWSLT
                     FTPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYKYRGIGMRDSN
                     NPKYYVWDLEPSYVENNLATDIKVAEITGKANNYTDGKVSEINSQLTASINEVDTTAK
                     DAQTKANANETAIKSTNSRVNEIENIITALTKEETYLLPASIVSGGSLKFTSRGGMTV
                     ATGSFKLAKDIAFGEVLVNDLPDSMINIDNNAIGTGAVTGNSVSQIYVESNTRKVVAN
                     TAMKKDQWFTVGFSYHSKLSI"
     gene            complement(4962..7253)
                     /locus_tag="IVN13_002334"
     CDS             complement(4962..7253)
                     /locus_tag="IVN13_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012790.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIIEGRIIQCYVDENRAKQRLRIYYAKTSVIGNMIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHETRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVEDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVNNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQDGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYDTAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNKPFLSFGAPNFR
                     YSNASYITDGDGSSLSINGSAGSSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKIA
                     RFEQSTVQVYKNFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDVDHFIIETSKPNVLVRWNLVAHRL
                     GYEDVRLKEDTAYDSTVLDQKRF"
     gene            complement(7263..8000)
                     /locus_tag="IVN13_002335"
     CDS             complement(7263..8000)
                     /locus_tag="IVN13_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7012791.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDAEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFSLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            complement(8050..>11214)
                     /locus_tag="IVN13_002336"
     CDS             complement(8050..>11214)
                     /locus_tag="IVN13_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP7012792.1"
                     /translation="STEVQKLERELINQQTAQQRLSNEIDKTSNALAQAKGEIQTYES
                     TMQQLDSEQKNVQASASLIESEYKKWQATAGQSASEAEKLAKAQEYVSQQSENAEKTI
                     DILRRQLEATQSEFGATSTEAMQMEAKLNDAEREFEELGQAAKNVDTTNLDDIGSKID
                     MNNLMEASDVLSDIGDKLTELGKQAVDSANSVGSSQSKIQANFGLSKQEAEELTNVAR
                     DIYYKGFGESLDQSTDALILVKRNLGDLNNQDLQNITEQAMVLENTMGADMDETLRGV
                     NGLMVNFGLSAQDAMDLMVSGTQNGLDKTHELGDNMAEYSQLWSQMGYSADETFGMLQ
                     NGLDAGAYNLDKVNDLVKEMGISLTDGRFEQNMDMFSESTRKAFEEWKNGGGTQKDVI
                     NSMIQDFSNMDGQYDQLNKASTIWSALGEDNAMKVVQSLTDVNHTFDDVSGSAQKMNE
                     DSTTPLQELNGKIAELKDSLAPIGNTIIDALEPVIDFLGKMADAFNNLPQPVQDFIVA
                     IGGLTAAFGILLPAILAVSYLFGPMMLIIGGIIAVIAGVIVAIKNWGAITDWFSGLWK
                     KFTDWLGDTWESIKDGASSVWDGVKETWSGFVDWVQDIWQGVSDWFGELWSGLVEGAS
                     NIWQGVQETWQTFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIV
                     GIFDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWW
                     NGIWTAISTTASNIWNSISATASSIWNSIKNTITSLVQAAATVIQNIWSTVSSWLGGI
                     WNSISSTASNIWNSVTSSISNAINAAKSAIQSVWNSISSWISGIWNGIKNTALNLWNG
                     ITSTISSKVNDGKNAISSGWSNLTGIVSDIFNNVKSTIANIWEGIKKTVSAPIDWIRD
                     KISGIFDNLNISIPHIPLPEFIMEGSFNPLKGQIPHLRVKWHAKGGIFTKPTLLGGMN
                     GVGEAGPEAVLPLKRSVLQEIGDRILSSTSVSSRAQTIQPVNNYEFNFTIDGNADEVT
                     MKQTTQQIIDSITKVQNDNASAWR"
BASE COUNT         3236 a         2375 c         1752 g         3851 t
ORIGIN      
        1 tatttagaat tttgcacatc gctgatatgg aatatttatg cttgttggca tcaattactt
       61 gtcttttcgt ccgaatatca gcgccgcttg ctttaaaata tcattctcca tttcgagttg
      121 cttatttttc tttcttagtg cgatcaattc tgcttgttct ggtgttaagt tgtctctttc
      181 tttgaatgag cccgttgatt gtgcttgctt catccattta tcgaaagaag aaggcgtaag
      241 ctcatactct cgaataattt ctgcgcgagg cttaccagcg agatagagat cgacgatttg
      301 ttgtttgaat tcttttgaat aggttcttcg ttgacgtctt gacatgaaaa atcctccagt
      361 gtgtttttta ttattctaca caccttattt tttctgtcta gtctagtgta gccgattcat
      421 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
      481 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      541 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      601 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      661 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      721 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      781 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      841 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      901 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      961 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
     1021 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
     1081 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
     1141 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
     1201 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
     1261 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
     1321 atggaaatct tgcgcaccgc ctttagtgtt tccctctgga tcattcgttg cccgcatcat
     1381 tacatagact ttcttactag gaaaattacg catattgaaa gatacatgat aaccaacgtt
     1441 tccagaagta ttataacctt gatttacatc tggtctataa attccatcag ctcttactcg
     1501 agctaattct ttcccagtat tgtagtccat aatgaaagta tactcgtatt tatagttagc
     1561 aatgtgccat ccagccacat gcaagtttgc gttttcgatt tctccaaact gatcaatgtg
     1621 ggcgtaattt gttccatctg ttagtgtagg atttgcagca cctgctcgag ttggatcaat
     1681 tactggttta tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata
     1741 ggctagtttt tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc
     1801 gccccaaata ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt
     1861 gtctaatgtt aatggaatac catatttcat tgctgaatct ctagctaatt caacgtatgc
     1921 cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt tgtaactcaa tctgcacggg
     1981 actgttggca ttagcatacg aaccagcccc atactgcaca taaccaggtt gaccgacttg
     2041 ataaacattt ccgccgtctc ccacaatgta agcagtgtaa gcactagtcc atgaacgttg
     2101 catatactgt gcttcattgc gtcctgttgc tgtttcgtta gctgtttcat gcagtaaaat
     2161 atactgatta ttcgctactt gagagctacc ttcgtttggc cctaaattaa attcattgtt
     2221 aatcgtatag gcaaacccat taattggcaa taaaaaaaga gccgttaata ggctcatcgc
     2281 agtaatagta attttctttt tcatttgttt cctcctattt tttcaaatta taagccgaca
     2341 caccagtgat aacacctaaa aatgttgctg ctgcattgat agtcagaact gccatatctg
     2401 ttccattcca tccatatgct ttgcctagtg ttgcaactaa tacagatgta gccggaagta
     2461 ccgtaagtac cgtccattta atgacttgat aatacttatc gggtaaaatc atttcttctc
     2521 acctccttta caatttagtc aagaaatagc caatgatcgt aatgcctaaa ccgatcatgt
     2581 aaccccacga ccatttatta ttggctttca tttctttgat atcttccgca ttattaagcg
     2641 caatagaata tgcctgatcc gctctatctt ttgcactttc cgctttttcg cgtaatgatt
     2701 cgtaattatc caacttcgtt tcaatacgca ctaaacgttc taccacgtct tgtattgctt
     2761 catctttcaa ccaactagcc tcctttcatt gcaaaataaa aacgtactcg aatgagtacg
     2821 ctaaatctat atagataatt tactgtgata cgaaaatcca actgtaaacc attgatcttt
     2881 tttcatagcg gtattcgcaa ccacctttct cgtattagat tcaacatata tttgcgatac
     2941 agaattacct gtgacagctc cagtccctat agcattgtta tcaatattaa tcattgaatc
     3001 tggcaaatca ttaactaaaa cttctccgaa tgctatgtcc ttagccaact taaatgatcc
     3061 tgtagctact gtcattccac cacgacttgt gaatttaaga gatccgccac ttactatact
     3121 agcaggtagc aaatatgttt cttcttttgt tagggccgta ataatgtttt ctatctcatt
     3181 aaccctacta ttcgtactct ttatagcagt ctcattagcg ttcgcttttg tttgagcatc
     3241 cttagctgtg gtatctactt cattaattga agctgtcaac tgcgaattaa tctccgatac
     3301 tttcccatcg gtataattgt ttgctttacc tgtgatttca gcaaccttga tatctgtagc
     3361 taggttattc tcaacatagc taggttctag atcccataca taatattttg gattgtttga
     3421 gtctcgcatg ccgattcctc ggtatttata ttgctcaata ttcggggttc gagtgtcacc
     3481 tttttcaatc ttgagccagt caattcggca tgcgccggcc gtttctttag gcgactgaaa
     3541 aatgcgaagg tctttaggca aacccggttc aagtttcgtt ggtgtgaatg ttagagacca
     3601 tacgtctgtc aatccctcaa ctggttttag atctccaaaa ttaacgttcc aataattata
     3661 tgctacaaag gtttgacttg cgggttttgt tcctttaagc gtgatagtat acgtttgacc
     3721 tattataagc tcttcttccg tgttaccttt atatatttcg tacgcgctag atttaattgg
     3781 gaacgtcgta cctttatttg caatattctt acccaacgat gttttaccta cccagtaagg
     3841 gtcatctagt aagtttggct gatacggagt ggctgttgag ccttcttcga ttttgatgtc
     3901 gtacaattta aatccaccat ttattttatc cctgttagaa aaactaacac taatataaaa
     3961 acgatctaaa tttgtgattt gataattaac gttagctgtg cctttgattg ttatttcttt
     4021 ccctacatca tctattgtaa tagttgtatt atttgcttcc aataatatat tttctcctgg
     4081 tgatgtacga tataccaaac gtaatttatt aatagctcct gtagttcctt catcaaatcg
     4141 aacttttgca ctcagagtat atgttttgcc actagcaagc tgaggtgtat taatacgcgt
     4201 aaacatatca atgtcccctg taccatcaga cataaaatgt aacattccgt taacattagt
     4261 tagtgaccca tggtaaccca tataaaaatc gctagattcc aatttattta caaataagtt
     4321 gggattccca ctataatcat agtcaccgaa gtcgatgctg ttactgtaca tcacttgtaa
     4381 gttacctaac ttagaaattt cttctttcag agcatctaac ttgtcttgta gtgttttagc
     4441 ttgaccagtt aaatcagtaa tctgttgatt taagctatcc actctacctt taatttcagc
     4501 cataaaagca tcaaaagttt cgttatactt tcgaatcaac tcttccaatt gcgaaacgta
     4561 ttcatcagct tggccttgcg aaatgtcaga cactcctagt gagaaaaaaa tgatgtcttg
     4621 cgttgttaaa atttgattac cttttctata ttctacgtag cagtgtttat aatatcctgc
     4681 ttcactcata aatgtgccat caagagaaaa tgtgacttct tcactagtta tactagttgc
     4741 aacactatct acataacggt tagatggcgt tgttcctttt aaagtaaatg ttccgccact
     4801 cgtatccatc tgcaagccat ttaaaaatgg tttaacagtc accgtaattc ctttgtcacc
     4861 ttgacgagcc ataatagctt tggtgtagtt taattcttta ctgaaatcta gagccaaatt
     4921 atataaactg ctagccattt atatacctcc ttgtcttcgt tttaaaaacg tttttggtca
     4981 agcactgtgc tatcatatgc tgtatcctct tttaatctaa catcttcata ccctagtcga
     5041 tgtgccacta aattccatct aaccaatacg tttggcttac tagtttcaat gatgaaatgg
     5101 tcaacatctt catgagtaac agcacacaaa actagttctg taggtgtcac atgtgtcata
     5161 taccgactta gatttactgt ctcagcaaac atgggatcaa tatcaacacg aactttacca
     5221 tcatcacctg taacggcttc tccataatca gcgaaataat attctggagt ttcataagcg
     5281 ttcaatagtc gttgtccata atgttctgtc ggtacggttg agtttttagt acctctaaca
     5341 gtaaaatttt tatatacttg taccgttgat tgttcaaacc ttgcaatttt cccatcttcc
     5401 catgaaccaa aaaaacaacc tggcaacgtt agcataccat cactagtaaa tttcatagtc
     5461 ctgccagcta ctttaaactc ccatgaacta cccgcactac cattaatgct taaagaacta
     5521 ccgtcgccat cagttatata actagcattg ctatacctga aattgggcgc accaaaagat
     5581 agaaacggtt tgttattgcc attatcccac gtactaaaaa ccaaattccc ctgtggattt
     5641 ctaatcatga aaccaccacc agttttcatg gtgtatgata caataccggc atcagcactt
     5701 acataatcac gtgcttctag ctccataata tctttgttaa ctttttttga gtaccaagtc
     5761 attttgccat tggcaatact tgttctataa tcagtaccat cactaattaa tgtagtacct
     5821 ctaatagtaa tccctactat ctcaccagct gtaataaacg aggcattaaa tccgccatct
     5881 aacgtccatg cggtgtcata cgttccatta ataccagttt tagagaaacc aataccagca
     5941 ttgttgattt gtaaaacatt ccttgcggta ttcttatctg gcgtgtccat aatcaaaata
     6001 cgactaggtg cttctttagg atctagtaaa acatacccac catcttgacc agtaatcatg
     6061 tcagtttgat gatctacaat atcattgatt aaatcactga tttcgccacc atttttcaat
     6121 tgatcaatgg catcattaat caaattgttg acattattct ctgtattttc taagaagttt
     6181 gttttgacgt ttcctacaac taatttatcg tatgaattgg ttaaaacatt aaacgtatat
     6241 tccacaattc ttgctgacat attcactttt aactgtggat gatacacatc tactccgtca
     6301 cccatcgaaa ctttttctag atcaacaaat ttttcatagc ctctttgatg cctcaatggt
     6361 actaattcaa tcgaaccact cacttgtggt ttctgtttat ctatgtttgt tttcaaccag
     6421 tctttagcag cttcccttaa tgtggccaca tcagtcgctt tgtctttaaa atcaacaaaa
     6481 gaaacatatc cagcaggata atcttccacg taatccgtga aaatgacttc ttctggtaga
     6541 gtgatctcat cctctccttc tgacgaactg ctaatgaatg gataaactcc aactaaaaca
     6601 ctttgagcat ctatctctaa gtcgagacca gttaagtttt tagtataaat cgctttgatt
     6661 ttatgatccg tacctagcct tgtttcatga cgtaatgtgt tattatcttt aagaaattcc
     6721 ccatgaaatc gatctaggat agatccttcc tttccgccaa agaattctaa aaaattcgcc
     6781 ttttctatct tcacattagc aagcgtatct actaacgatg agaaagaaaa ctgcgaagga
     6841 atagctggtt ttgctaaaac ttttgcgttt tgccatgcct gagtagcagt gatcttttct
     6901 gttccgctgt catatttatt caacaccgat tttcttatat cattgaaaat aggttcagct
     6961 tttacttcta tcatatttcc tattacagaa gtctttgcat aataaatccg taaacgttgc
     7021 tttgctcgat tttcatccac atagcactga ataatacgtc cttctataat caagtctgca
     7081 ttagttccgc ttattggata agtaccctga aatatctcgg ctccgtttaa tttattgcta
     7141 acagtggctg ttaaccagtc tgacaaagcg cctaaacctt gtgtatcata taaatgttca
     7201 gctaaattat tcgcgtcatt tttatcgtaa atagttatta aattatcgat catctatttc
     7261 acctacctta acccattacg ataaatttgt attttgctca aaccagtaca attaaaataa
     7321 ttaatatcca cttgcaatgt cggatattgc atggtcttca ttttgttgga tcgatctaaa
     7381 atatctccgt ccgattgctc ttcgtagcaa agcattaaat cactatcaat gactacatca
     7441 gttcctacta ctaagccttc aaaactaaac acataatcat ttaagatgaa ctggcatgaa
     7501 gtagctgaag gagtgatgat aatctttgga aaactttctt ctaaactatt attcagcaaa
     7561 ctaaatgact gtggtttatc tacggttata ggcacatctt cttgaactct tgcgaatggt
     7621 ttcgcagtaa tatttacatc gaactctccc cattcaacaa tatcgttttc tgcatcccca
     7681 atatcgatag tctggataac ataatagacg ttgggatcat cagagaattc taatttcttt
     7741 gcatagttta accaatgacg catgatataa aacgattgct tgaacgcttg atggtcttcc
     7801 gcatcctcta aatagttata gtgcaaagta aacgacatat cttcaaacga ataatcttgt
     7861 actaagccac ctaaccgtcc taaaacagaa gtttcaactc tctgtctttt tggagatggt
     7921 atggttggtc tttcagctaa agccaattta tgcaaataat caggaaatcc atcgattata
     7981 gaatgtatac aatcagtcat tttttcacat cctttttaat actaaaaaac aggagaaata
     8041 ctctcctgtt taacgccatg ccgaagcatt atcattttga acttttgtaa tgctgtcaat
     8101 gatttgttga gttgtttgct tcatagtaac ctcatctgcg ttaccatcaa ttgtgaaatt
     8161 gaattcgtag ttattcacag gttgaatcgt ttgtgcccta gatgaaactg atgtgctact
     8221 caagatacga tcaccaattt cttgcaaaac agatcttttc aaaggtaaaa ctgcttcagg
     8281 tcctgcttca ccgacaccgt tcattccacc tagtaaagtt ggtttagtaa agatacctcc
     8341 tttagcatgc cattttacac gcaaatgggg gatttgacct tttagcgggt taaagctgcc
     8401 ttccatgata aattccggta acggaatatg tggtatagaa atattcaaat tatcaaaaat
     8461 gccactgatt ttgtctctaa tccaatcaat tggagcgcta acagtctttt tgatgccttc
     8521 ccatatgtta gcaattgtac ttttaacatt attgaatatg tcggaaacaa tacctgttag
     8581 attggaccaa ccgcttgaaa ttgcattttt tccatcgttt actttagagc taatagtgct
     8641 tgtaattcca ttccaaagat tcaaagcagt atttttgata ccgttccaaa ttccgctaat
     8701 ccacgaagat atactattcc aaacactttg aatggcactt ttagctgcgt ttatagcatt
     8761 gcttatacta ctagtcacac tattccagat atttgatgct gtagagctga ttgaattcca
     8821 aattccacct aaccaactag atacagttga ccaaatattt tgaattactg tagcagctgc
     8881 ttgtaccaag ctagtgattg tattcttgat actgttccaa atactagaag ctgttgcact
     8941 aattgaattc caaatatttg aagccgtagt actaatagct gtccatatac cattccacca
     9001 tgccactact ggatcaaata tagtatggaa tgtagttaca atcccattcc aagcgatact
     9061 tatccattgt gtcatagtat cccaagtatt tttaaggaaa tcagaaatag gtgtccaaac
     9121 agcttgccaa gctgcgccca ataactgtcc agctacatca aaaataccca cgataatatt
     9181 aataccagct tgaatcaatg acgttattaa tgtccatggt atttgaacaa ttcctacaat
     9241 gtctgcccaa ataatcgacc atacttcttt gactccgttc caaatatttg aaacccaatc
     9301 aacgaatgtt tgccaagttt cttggactcc ttgccagatg ttggaagctc cttcaactaa
     9361 tccgctccat agctctccaa accaatcaga aactccttgc caaatatctt gaacccaatc
     9421 tacaaatcca gaccaggttt ctttaacccc atcccaaact gatgaggctc cgtcttttat
     9481 actttcccaa gtatcaccca accaatcagt aaattttttc cataaaccac taaaccagtc
     9541 agtaattgca ccccagttct taattgccac aataacgcct gctataacag ctataattcc
     9601 tccaattata agcatcatcg gaccaaataa atatgacacg gctagtattg ccggtaacag
     9661 tatgccaaat gctgctgtaa gaccacctat agcaacaatg aaatcctgta ctggttgtgg
     9721 aagattatta aacgcatcag ccatctttcc tagaaaatca attactggtt cgagtgcatc
     9781 tatgattgtg ttgcctatag gagctaatga atcctttaat tcagctattt tcccgttcaa
     9841 ctcttgcaac ggagtagtag aatcttcatt cattttttgt gcagatccac taacatcatc
     9901 aaatgtatgg ttaacatcag ttaaagattg gacaactttc atcgcattat cttcgccaag
     9961 cgcagaccaa attgtcgaag ctttatttaa ttggtcgtat tgaccatcca tattgctaaa
    10021 atcttgaatc atggaattaa taacgtcttt ttgtgttcct ccgccatttt tccactcttc
    10081 aaaagctttt ctagtacttt cactaaacat atccatgttt tgctcaaatc gaccatctgt
    10141 taacgatatt cccatttcct taactaagtc attgacttta tcaaggttat aagcacccgc
    10201 atctaaacca ttttgaagca ttccgaacgt ttcatcagct gaatatccca tttgactcca
    10261 taattggcta tattctgcca tattgtcgcc taattcgtgc gttttatcta aaccgttttg
    10321 agtacccgaa accattaaat ccattgcatc ttgagcactc aagccgaaat tgaccattaa
    10381 gccatttaca ccacgtaacg tttcatccat atcagcgccc atggtgtttt ctaggaccat
    10441 agcttgttcc gtgatatttt gtaaatcttg attatttaaa tctcctaaat tacgctttac
    10501 caaaatcaat gcatctgtgg actgatctaa cgattctcca aaacctttat aataaatgtc
    10561 tctggctaca ttcgttaatt cttcagcctc ttgtttagac aaaccaaaat tagcttgtat
    10621 cttactctgg gaactaccta cactgttagc agagtccact gcttgtttcc ctaattctgt
    10681 aagcttatcg ccaatgtcgc ttaaaacgtc agaagcttcc attaaattat tcatatctat
    10741 tttgcttccg atatcgtcca agttagttgt atctacattt ttagcagctt gtcctaactc
    10801 ttcaaattca cgttcagcat cattaagctt cgcttccatc tgcattgctt ctgtggatgt
    10861 agcgccaaat tcagactgtg tagcttctaa ctgtcgtctt aggatatcta tcgttttctc
    10921 cgcattttca gattgttgag aaacatattc ttgggctttc gctaatttct cggcttcaga
    10981 agctgattga ccagcagttg cttgccattt tttgtattcg gattcaatca gagaagcact
    11041 agcttgaaca tttttttgtt cgctatccaa ctgttgcatt gtagactcgt acgtctgtat
    11101 ttcgcctttt gcttgagcta gtgcattact cgttttatca atttcgtttg acaaacgttg
    11161 ttgcgctgtt tgttgattaa tcagttctct ttcaagtttc tgaacttcgg tgga
//
LOCUS       DACCKU010000092        10812 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.095, whole genome shotgun sequence.
ACCESSION   DACCKU010000092 DACCKU010000000
VERSION     DACCKU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10812
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.095"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..254)
                     /locus_tag="IVN13_002337"
     CDS             complement(<1..254)
                     /locus_tag="IVN13_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP7012793.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADK"
     gene            complement(272..592)
                     /locus_tag="IVN13_002338"
     CDS             complement(272..592)
                     /locus_tag="IVN13_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7012794.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            891..1667
                     /locus_tag="IVN13_002339"
     CDS             891..1667
                     /locus_tag="IVN13_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP7012795.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            1682..1885
                     /locus_tag="IVN13_002340"
     CDS             1682..1885
                     /locus_tag="IVN13_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7012796.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            1901..2239
                     /locus_tag="IVN13_002341"
     CDS             1901..2239
                     /locus_tag="IVN13_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP7012797.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            2226..2405
                     /locus_tag="IVN13_002342"
     CDS             2226..2405
                     /locus_tag="IVN13_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012798.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(2448..2918)
                     /locus_tag="IVN13_002343"
     CDS             complement(2448..2918)
                     /locus_tag="IVN13_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012799.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            3005..3703
                     /locus_tag="IVN13_002344"
     CDS             3005..3703
                     /locus_tag="IVN13_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7012800.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            3881..4222
                     /locus_tag="IVN13_002345"
     CDS             3881..4222
                     /locus_tag="IVN13_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012801.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            4215..4886
                     /locus_tag="IVN13_002346"
     CDS             4215..4886
                     /locus_tag="IVN13_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7012802.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            4892..5578
                     /locus_tag="IVN13_002347"
     CDS             4892..5578
                     /locus_tag="IVN13_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012803.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            5581..6330
                     /locus_tag="IVN13_002348"
     CDS             5581..6330
                     /locus_tag="IVN13_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7012804.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPIRHKYGEYKNVLLSDEQME
                     KLKTEFPNDHQERIERLSEYCESSGKTYKNYLATIRSWAKKEKSEPKNTSSGYKRTGR
                     REKLPEWAIDQEAYQKKKALERANRQSKAPF"
     gene            6342..6611
                     /locus_tag="IVN13_002349"
     CDS             6342..6611
                     /locus_tag="IVN13_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012805.1"
                     /translation="MKIDYLELINEIASYKTGEEIEILRDVYEQLDEAGIERIKNDRS
                     SWSKLRYYFALYIDATQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            6773..7075
                     /locus_tag="IVN13_002350"
     CDS             6773..7075
                     /locus_tag="IVN13_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012806.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNENEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGNKLLVTGTAEQLASLTGLHEKIVRKRARCGYTDVKKRTFRYVEESS"
     gene            7072..7233
                     /locus_tag="IVN13_002351"
     CDS             7072..7233
                     /locus_tag="IVN13_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7012807.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIVNL
                     RREIRGLEE"
     gene            7230..7535
                     /locus_tag="IVN13_002352"
     CDS             7230..7535
                     /locus_tag="IVN13_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012808.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRRIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            7535..7852
                     /locus_tag="IVN13_002353"
     CDS             7535..7852
                     /locus_tag="IVN13_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7012809.1"
                     /translation="MDLITQYSDIILKKIMAKIQKDKKSKERAELVKLEMAETGVGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILEEYKE
                     DSK"
     gene            7849..8235
                     /locus_tag="IVN13_002354"
     CDS             7849..8235
                     /locus_tag="IVN13_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012810.1"
                     /translation="MIPKFRAWYTPFKGKTIGQEMKYGQAGRLITHAEMAPDKYVLMQ
                     STGLKDKNGVEIFDGDVVQAEQYLTTTIPVRINGIVKYSNRYTMFYLDNGSERHDLYM
                     QSLGGSIYNFEIIGNIYENPELLEEQ"
     gene            8235..8948
                     /locus_tag="IVN13_002355"
     CDS             8235..8948
                     /locus_tag="IVN13_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP7012811.1"
                     /translation="MNKQELIEKLAKYVKRYENVMDEHGQGRYCAYEVSLKLAKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGESQKPVMPKLFDDWAKRVIAKH
                     DEFYAISLVARAGWGYGVDFELSENGSSSENKELLYWLVDKCSDNYPNKKKAIEALLY
                     GYEVEKEPLYHVKLKIPGVTYYLIQTFLMPGDTHMCFSAATERYGAKWKNTFTESEIK
                     AIDERYWPFAVPVEEEAEG"
     gene            8945..9322
                     /locus_tag="IVN13_002356"
     CDS             8945..9322
                     /locus_tag="IVN13_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012812.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQAKMTQISHLRLEL
                     EDLRQIEKSIQLNDNQQIVFEWLKLTAPTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKEQFEVLAAFAQWGLEQEEAE"
     gene            9319..9549
                     /locus_tag="IVN13_002357"
     CDS             9319..9549
                     /locus_tag="IVN13_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012813.1"
                     /translation="MKYEIPLSEADVQSIINGREVNKKLPDGTELVIRQSYLKDMAAP
                     VLIDRFNVTDSVVENHLKEFRSSINDTFRLGS"
     gene            9556..9759
                     /locus_tag="IVN13_002358"
     CDS             9556..9759
                     /locus_tag="IVN13_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7012814.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASDAIRKFGISILNDDRKWEEMR"
     gene            9756..10052
                     /locus_tag="IVN13_002359"
     CDS             9756..10052
                     /locus_tag="IVN13_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012815.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKWTAKSKENLAVARLVDMELINDK"
     gene            10128..10541
                     /locus_tag="IVN13_002360"
     CDS             10128..10541
                     /locus_tag="IVN13_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7012816.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
BASE COUNT         3996 a         1679 c         2259 g         2878 t
ORIGIN      
        1 attttatctg cttctgtttc aaattttgag tgaaaagttg tagaagtatc gtacaaaact
       61 gaaaattcaa aatgagaggc gctgaaatgt gcaagttcat gagataaatg aaaggcttct
      121 gcagtttcac cgtatagatt ttcattcaaa aaaatgattc tgggttttgg atagtagaaa
      181 ccaggctctt tcatctccat ataaactact ttcaaattgt attcgctcaa catttctttc
      241 aatttcaaat acatacaaac catcactcca actattcatt ttcctctaaa gctttagcaa
      301 ttgcaatcgc tttacgcatt gtctccttag atatttcttt tccatcaaaa gaaaaaacag
      361 tatcgtcttc tgataaatcc acatgtttag gggtttctct ttcttctcta cctagaaggt
      421 agtctacaga gacatcgaaa tagtcagcaa tccgacttaa ttcatctgaa ttaggagtat
      481 tatttttcca ctttgctaga taaccgttag aatatccaaa attaatttct aactgccgta
      541 tagatatctt tttcctcttc gccaagtctt ttattatttc ataggtattc atcgataaaa
      601 caacctttct aaatgtttac aaaaaaagtt tagaaaaata cgcagaaata atttgactaa
      661 ttctgagtaa tgagctatac tatatcttgt aaacagattt aacaactaaa aagacaacaa
      721 aaaacactat tgatatgtaa atgcagaccg ccaagaaagc tttaaaatca atgtttttat
      781 gtcttattta attatgctct tagtatagag tattactcag atactgtcaa ttgaatttag
      841 aaaaaagttg ttaaatttgt ttacgaatat aaaagaaagg aatgagaaat atgaacacac
      901 cgcaaatttt taatttcgaa caaaacgaag ttcggacaat tttagtaaat gatgaaccat
      961 attttgtagg caaagacgtt gcgagtgttt tgggttattc aaacactaaa gatgctttgt
     1021 cgcggcatgt agatttggaa gataagatgg ggtcgcgaat cacgacctca ggtcaatcaa
     1081 gagagatgac aatcatcaac gaatctggtt tgtacagttt aatcttaaaa tcaaaacttc
     1141 cctctgccaa aaaatttaaa cgttgggtaa caagtgaagt gttgccagca attagaaaac
     1201 atggaggtta tctaactcca gaaaaagtag aagaagcttt gcttaatcca gatacaatca
     1261 ttcaattagc aactcaacta aaagaagaaa gaactggaag attaatcgca gaacaaaaga
     1321 ttgcagagta cgaacctaaa atctcctatt tagatagcat attatcttct acagattcag
     1381 taacaattag tcagattgca gcagattatg ggatgtctcc acaacagatg aataaattac
     1441 ttcataaact aggtattcag aaaaaagtcg gtaaccaatg gttattgtgc aaaaaacaca
     1501 tgaaccaagg atacacaaaa tctcatacaa ctgagatccc gaaagccgat ggtggcacta
     1561 aaattgtaat gaataccaaa tggacacaga aagggcgtct atttatctac gaattactaa
     1621 aaaaagaagg atattaccct caaatggatt tagaggaaat tggttagaaa ggagttttag
     1681 tatgactgac attgcagaaa tcactcaacg agatagagaa aaaatcaaag aatatgtcga
     1741 aagttcaaag ttcttaactt acaccatgct tgctgaaaga tttggaatta gcaaaagcta
     1801 cttatcttta attttaaacg gtaaaaagac ttctgcagaa gcaaacagaa ttatagattc
     1861 gattatcact atgtacgaat tgtaaaggga ggaacagcta atgcaatatc tagaagcaaa
     1921 aatcccaatt ccagaaggct atgtaattat ctcccaagtg gattatgagg agttaaaaaa
     1981 agctgatgat actggtagat ggatgacgtt gccagaagtg ctagaacgga ttaacagaaa
     2041 atatgattgg tttacttcta gagttttaaa gaacccaaga tatagaaata ttatcgatat
     2101 cgagaaaaac aaaaatggat ttgtctatta tccagttgaa ggaagagaca catatctatt
     2161 tttaagaagt aaaacacttg aatttttaga aacaaatttt tcggaaatct taaggaggca
     2221 agcggatggg aaaatttaat agagcgctag tgttcagcgc accgctaatc atctacgctt
     2281 taggactttg gggaagcagg caagcgttga taggaacgat tgtttacatg gtttggattt
     2341 ttatggggct tgatgaagct gaagctgagt acagagcgaa aaagccaacc gagggggctg
     2401 actaaaaatg aatagaaaag agaaactaga atgtatatta ctattactca gtttaattct
     2461 ttcactaatt tctctattgg gaagtttgta tttttgatat caacaatttt gtggatttgg
     2521 gttctattta gagtaaatat catttttgtt tggtgattaa ggagtttatt aaaattaatt
     2581 ttccctactt caaatgcaag gtaacaagat atagcacttt taggaggaat atttagcggt
     2641 acagcttcag agtatattct attatcatcg actgatcctc ctttagcaat taatttctta
     2701 aatggagtag ctgaccatct gcggtttaag ttagcggatg aaagttctaa attaactaac
     2761 atagctggtt ctgttgaata gttagaaatg attacttttg tataaatcat atctgcagcg
     2821 aagtaagaag cattaagttc aacttctagt tgagggcgca ttccttttat ttttatccct
     2881 gttaaaaacg tactcaatat aaaacttatt attgacatcc attgaaaaat tgttaagtga
     2941 aaaaaattca aaataaacac caccagtttt taactaaatt ataccaaaaa ggagagaaga
     3001 aataatgcaa gaattagtaa ttttgaaaaa taaagaagct gtgactacga gcttacaagt
     3061 ggcagaaagc ttcgagaaaa aacatcaaca tgttttaaga gatattgatg cactaaaaaa
     3121 agatgtgtcc aattttggac agatgtttgt agaaggtaat gaaccagatt catatggcag
     3181 aaatcgacga gttttcttca ttagtagaga tggatttttc ttgctggcta tggggtttac
     3241 aggaaagaaa gctatctact tcaaacaaaa atacattgaa gcattcaacg aaatggaaga
     3301 tgttattcgc aagaatactg ttcctcaaac aattgaagat atgatgatct atcaattaga
     3361 agaaatgaaa gatgttaaaa aagatgtttc catgcttaaa gatactatgc gaattagcgg
     3421 acaacaagag tttgaaatta agcaaaaagg aaatatgaaa gttatgggag ttctaggggg
     3481 aaaagaaagc cgagcttatg aagaaatcag caaaaaagta ttctcaaaat tttggtctga
     3541 atttaaacgt accttttcaa tcccaagata tggcgagtta cctcgtaaga gattcgatga
     3601 tgctgtttca tttattgaaa tgtggttgcc agaaactgcg atccgtatgg aaattgatca
     3661 actgaacaga caacagagac tttttggtga tgacaatgaa tagagctgaa gcgctaagaa
     3721 tagggacggc aattgctaat tgctggtgga aatactataa accaatcatc ctaagccaac
     3781 aacatattga caagcaaaaa gcatggcaac aaataaaaaa gtgactccgc cggcaagcaa
     3841 agagtcacaa agaaaacaca tcataaggag attttagcat atggaaaaag aactttccac
     3901 tctagatcaa tatttgattg atcctgattg gggcaagccg aaaattgagg aaacaagtgg
     3961 tcgaaaaatc agacgaaatc ttttgacgaa tgaagaacta gcttgggatc aagatgattt
     4021 aggcaaccat gtaactattt gggatcatgt ttatcttatc catctatcga agcattcgaa
     4081 taaacctgaa tatatttacg tcatcgaaga tggcttgatt gatgcgctag aagagtacga
     4141 cagagataac ttgattgata tctcttatta cggaccaggt aagaaataca ttgctgaaat
     4201 ggaggcagaa tttgatgagt gaaatcaaag ggacaacgaa ctttgaaaaa ctttttagtc
     4261 gtaagttaaa taaaattctc aagaaaaaag gaaattttga ttatttatct tgggctcacg
     4321 cgtgggagat tatgaaaaag aatgatccac aggcaacggt aactattaat gagtacaaac
     4381 actacagggt tgtttctgga actcatcaag actttcttgt tgaggaatat aaaccttttc
     4441 ttatggatga aactgggact tatgtatctg tctcagtaac ggttaaagga cacacggaaa
     4501 ccgaattatt tcctgtttta gattatcgaa accaaccagt tgttaaacca aatgcaatgc
     4561 aaatcaataa ctcattgaag cgatgctttg tgaaagcatt ggctctacac ggactgggat
     4621 tatatgtatt tcaaggggaa gatattccaa caccacctag aatcgataca aagaaattaa
     4681 acatgctaga gacgattcta gaagctttca atgagcagat gggtaaagat atgaccaaaa
     4741 ccttaatcga atatgttaat gagcagacag ataaattagg gctcttagct gataacgttg
     4801 aaactattga acagttaagc tatgagcaat gtgccttgat ggagcgagca atagcagcta
     4861 agagaaaaga attagataag aagtgatatg agtgtttaaa ccattaatcg attcatattc
     4921 agcggttctg aaaaagttca aaggaaaaga cataagcgca accatcaatg aggaagtgaa
     4981 cattgatcga ctaaagacga tgtatgacgg ctacgatggt gatcgagtca ttgaaattcg
     5041 ttttattgat cctagacgtt tcaccgtaca gcaacgaaac ttcatctatg cgctgatagg
     5101 cgatattttt atcgatacag gcatgccaac ggacttctgg aaggaattct tctactttcg
     5161 ttttgaaggt gtcacagggc gcaaaataag cctgaaagac gaatcgaata caactgtgag
     5221 tgatgccaat gtcttagcaa atatcatctt agatttcatc tttgaacatc atattccttt
     5281 caaagaaggc tatgagattt tacctgcgaa tcaagaatat tacttctaca aatgcatcac
     5341 aaaaagagtc tgctgcatct gtggcaaaac aggagctgac atcgatcact ttgacaaagc
     5401 gctaggaaga cgaaagcgca aagaagttga tcatgcagag tacacatttg cagcactctg
     5461 cagaatccat cacacagaga agcacaaaat aggtgtgatt aatttcaaaa ataaatatca
     5521 aatcaaaggg atcaaattaa accagaaaac aatcaaaaag ttaaatattg gagggtaaaa
     5581 atgacagaac atcgaagtta ttatgcgatt ataccagcca acgtaaggta cgacaaaaga
     5641 cttaaaccaa atactaagtt gttatacgga gagataacgg ccttgtgtaa tgaaagaggc
     5701 ttttgttggg caggcaatga gtactttgca gatttatatg gtgtgaataa agagaccata
     5761 tcacgatggg taagtgattt gattaagttt ggatacttga atcgggaaat catttacaaa
     5821 gagggtacca atcaaataat caataggtac ctacgaatta atcaataccc tattgacgaa
     5881 aaacgcaata cccctattga cgaaaaagtc aaagataata atacatctat taataataca
     5941 tttaataata caaaagaata tataagagag ttaccgcctt cgaaaaaatc gaaggctaag
     6001 cccatccgtc ataaatacgg agagtataaa aatgttcttt tgtcagatga gcagatggag
     6061 aaactcaaaa cagaattccc taatgaccac caagagcgaa tcgaacgact gtcagagtat
     6121 tgtgaatcat ctggtaaaac ttataaaaac tatttggcaa ctattcgaag ctgggcaaaa
     6181 aaagaaaaaa gtgaacctaa gaacacaagt agcggataca agcgcacagg aagacgagag
     6241 aagcttcctg aatgggcaat cgaccaagaa gcctatcaaa agaaaaaagc gctagaacga
     6301 gctaatagac aatcaaaagc accattctaa gaggtggaaa attgaaaatc gattatctag
     6361 aactgatcaa tgaaatagca agttacaaaa ctggtgagga aatagagatt ctgagagacg
     6421 tttatgaaca acttgatgaa gctggaatcg aacgaattaa gaatgatcgt tcaagttgga
     6481 gtaaactcag atactatttc gcactttata tcgatgcaac acaattaaga aatttagctt
     6541 ataccaaatt actatttgtt gattgtgtta aaggcttgca aaaacatctt aatgaacttg
     6601 agcaggtgta atcagatgga cctaaagaca tttacagcac agattgaact aatgcatcaa
     6661 gaagctttaa gacaaagtgt atcgtacgaa gacaagtggc tcaacacgtt ccacggcgga
     6721 cgtgagagcg cacttgatca agtactcaaa ttactgaaag gagaatgtcg ggatggataa
     6781 gaaagcggca atgcagcgaa ttatcgaatt gacttattca gaagattggc aaaatgaaaa
     6841 tgaagctgct tcagaagtga tgaggcttgg aagagcgatg tgggcagaca agagcaacaa
     6901 gccaagacca cgaaaaatcg caatttggca cggtaacaaa cttctagtga cagggacagc
     6961 tgaacagtta gcaagtctca caggcttgca cgagaaaatc gtgagaaaaa gagctaggtg
     7021 tggatacaca gacgttaaga agagaacgtt taggtacgtg gaggaatcat catgacaaca
     7081 gaagaagtga ttcaaatgcg tattcgaagc cttcaacgtg aaattgacga tctggaacga
     7141 acaaaggcag tgatggtcaa tgaaacggca aggaaggcaa tcgatttgca catagtgaat
     7201 ttaagaaggg aaattcgtgg attggaggaa tgagcgtgga taagaaagcg acaatgaaac
     7261 gaattgctga attaaccaag tcagaatctt ggcaggaaga caaagaaata gttgcagaag
     7321 tccaaaagct cggtaaatca atgtggactg aaaagcccaa acggaaaacg ccgagaagaa
     7381 ttgcaatctg gcatggtgat cgaattctag taacaggtac tgctgaacag ttatctgaaa
     7441 ttactggatt aagcaaaaac attatttggg atagagcaag gagcttatgg attgattcaa
     7501 aaggacgaca atttaggtat gtggaggaga aataatggat ctcattacac aatacagtga
     7561 tattatcctc aaaaaaatca tggcaaagat tcagaaagat aaaaaatcaa aagaacgagc
     7621 tgaattagtt aagttggaaa tggctgaaac aggagtagga gtgcgaagta gcaggcattg
     7681 gaaagcagca gcaaacattg aattttatta caacgaaatt caaaaagggt tcgatcaaat
     7741 gcgtgagctg gatcggcaaa caaattggag caagaaactt catcaagatc gtttcaaatt
     7801 tgtagaaaag tatagagaga tactagagga atacaaggag gacagcaaat gataccgaag
     7861 tttagagcgt ggtacacacc atttaaaggt aaaacaattg gacaagaaat gaaatatggg
     7921 caagcaggaa ggttgatcac tcatgctgaa atggctccag ataaatatgt gcttatgcaa
     7981 tcaacaggac tgaaagataa gaacggtgtg gagatatttg atggtgacgt tgtgcaagcc
     8041 gaacaatatt taacaacaac tatccctgtt cgtataaacg gcatagtcaa gtattccaac
     8101 agatatacta tgttttatct agataacgga agtgaaaggc atgatttata tatgcaatca
     8161 ttaggaggta gcatatataa ctttgagatc atcggcaata tctacgaaaa tccagagtta
     8221 ttggaggaac agtgatgaat aaacaggaat tgattgagaa attagctaaa tatgtaaagc
     8281 gttatgagaa cgttatggat gagcatggcc aaggaaggta ctgcgcttat gaagtatctt
     8341 taaagttggc gaaaagacta aatgaatcaa aaattacaga cgaacaagct tggaataagg
     8401 tagctgaggc ttatcctgaa tcggcacaaa gcttgagaaa cactttagat aatgctgtat
     8461 ttggtaagac tggtgaatcg cagaaaccag ttatgcctaa gctttttgat gattgggcaa
     8521 aacgagttat agcaaaacat gacgagtttt atgctatttc acttgttgca cgtgcaggtt
     8581 ggggatatgg tgttgatttt gaacttagcg agaacggatc atcatcagaa aacaaagagc
     8641 tgttgtactg gcttgttgat aaatgcagcg acaattatcc taataaaaag aaagcaatag
     8701 aagctttgtt atacggctac gaggtcgaga aagaaccgtt gtatcacgtg aagctaaaaa
     8761 taccaggcgt aacatactac cttatccaaa cttttttaat gccaggagat actcatatgt
     8821 gtttctcagc ggctacagaa cgttatgggg ctaaatggaa aaatactttt acggaatcag
     8881 aaataaaagc aattgatgaa agatactggc catttgctgt gccagtggaa gaggaggcag
     8941 aaggatgaaa aaattgcacc aactcatatc tgagaaagaa agcgaactcc aaaatttaga
     9001 agatagtttg ggattggggt ttccaattgt tgagcaagct aaaatgactc aaatcagtca
     9061 tctacgactt gaattggaag atttaagaca aatagaaaaa tcaattcaac tcaacgacaa
     9121 ccagcaaatc gtgtttgagt ggttgaaact aactgcgcca acaggaaagc caatgcaagt
     9181 tgtattttgg atgatgaata atgcagcatg ggggcattta gacgaattaa gggatccttt
     9241 gatggaatta acagacaaag agcaattcga agtcctagca gcattcgctc aatggggatt
     9301 agaacaggag gaagcggaat gaaatacgaa ataccactaa gtgaagcaga cgtccagtca
     9361 attattaacg gtcgggaggt taataaaaaa cttcctgatg gtactgaatt agtcatcaga
     9421 caaagttatt tgaaagatat ggcagctcca gtattaattg atcgttttaa cgtgactgat
     9481 tctgtggtag agaaccactt gaaagaattt cgatcaagta taaacgacac tttcagatta
     9541 gggagttgat tgacaatgaa caccagacac cgcagagtag caaaactaag aaaacaggaa
     9601 ctgaatgtac taaagacaaa gtttgaaaaa gaatatggaa tttcagcaga agaaacatat
     9661 aaagtggcaa gtcagtttgt tgctgatgca agtgatgcta ttcgtaagtt tgggatttcg
     9721 atattaaatg atgatcgtaa atgggaggaa atgagatgaa actaaaagac ggattttacg
     9781 ctagtagtca cggcatcggc ggtttaatgc tagatatgcc gacaaagaat cctaaaacac
     9841 gtaagaaacc aaaagtaaaa gttggtgaca tggttcgctg tgaagcagaa gggttcatct
     9901 atccatttcg tggatatgta gagcatctct ataatcactc agcgatcatt cgtattgaaa
     9961 acacgatgga atgtgataaa tggacagcta aaagcaaaga gaatttagct gtagcgagat
    10021 tggtggatat ggaactaatc aatgacaaat aaaaaagccg gatcgctccg actgttctaa
    10081 taaattccac aagtttatta tatcacataa aggagcggtt tgacttgatg caattgttac
    10141 gagaggtaga tttcaaacag acaagatgta atgcgagaga tgtgctgaag aactttcggc
    10201 gtttggagcg gatggcaggt cgctctttga tagatattaa gtcgccgatt ataacggata
    10261 tgccgaaggc accgaagcat ggcaataagg cagaagacgc gattattcag atgatggata
    10321 tagaaacgga gagagatgcg attctagcgg ctttgatggc tcttagtctg attagtcgtc
    10381 agatactcta ctacagcttc tgtgtgccag atagcttctc aaactacaga attagccgtg
    10441 aagtgggtta ttcagaaaga agtatacaac ggatgaagtc ggaagctcta atagagtttg
    10501 cagaagcata taaacacgga aaaataattg cttataaata atttggcggt tttttggcgg
    10561 aatgatggcg gtttttagac atttaccagt gttattatgg tagtgtcgaa agattaggaa
    10621 acaggatcga caaaataaac gaagggaggc aatctccctc atcgttttaa acttcttctt
    10681 agatagacag caactaatta tgaagaaaag gatgtgaatt caactcctcc taaattcttc
    10741 atcggttgct gtttattttg attagacagc agcactcaca aaactaaaca tgttcttgtc
    10801 gtctttcaac tg
//
LOCUS       DACCKU010000093        10534 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.096, whole genome shotgun sequence.
ACCESSION   DACCKU010000093 DACCKU010000000
VERSION     DACCKU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10534
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.096"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            55..279
                     /locus_tag="IVN13_002361"
     CDS             55..279
                     /locus_tag="IVN13_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012817.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            294..731
                     /locus_tag="IVN13_002362"
     CDS             294..731
                     /locus_tag="IVN13_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012818.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            724..1431
                     /locus_tag="IVN13_002363"
     CDS             724..1431
                     /locus_tag="IVN13_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012819.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            1812..1991
                     /locus_tag="IVN13_002364"
     CDS             1812..1991
                     /locus_tag="IVN13_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012820.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            2084..2269
                     /locus_tag="IVN13_002365"
     CDS             2084..2269
                     /locus_tag="IVN13_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
                     /protein_id="HAP7012821.1"
                     /translation="MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQ
                     DLGRGLEQKILKDAGLK"
     gene            2303..2734
                     /locus_tag="IVN13_002366"
     CDS             2303..2734
                     /locus_tag="IVN13_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAP7012822.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
     gene            3671..4945
                     /locus_tag="IVN13_002367"
     CDS             3671..4945
                     /locus_tag="IVN13_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7012823.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            4999..6426
                     /locus_tag="IVN13_002368"
     CDS             4999..6426
                     /locus_tag="IVN13_002368"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7012824.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            6439..7311
                     /locus_tag="IVN13_002369"
     CDS             6439..7311
                     /locus_tag="IVN13_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7012825.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            7406..8401
                     /locus_tag="IVN13_002370"
     CDS             7406..8401
                     /locus_tag="IVN13_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7012826.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(9006..9113)
                     /locus_tag="IVN13_002371"
                     /pseudo
     CDS             complement(9006..9113)
                     /locus_tag="IVN13_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            9229..>10534
                     /locus_tag="IVN13_002372"
     CDS             9229..>10534
                     /locus_tag="IVN13_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7012827.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVGEKLKISFEPTDSF
                     QYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAERTGINVFKNELNESRLKTYDTL
                     LNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDNSSYPKPISDYINYLNNMKNRYE
                     QKNVNNIASYIRDEIIMGKYEIDEKDNSVSFRQKFGKNRYRKERIPFHVVSSSIKSLY
                     GLDYYLENSSKEGDYLIIDEPELSLHPENQVKLAKVITDIVESKIKLITSTHSDLFVR
                     ELTNIVIENKLNSKNNKKNKLSDKNIGIYNFVGNNDINELEKI"
BASE COUNT         3783 a         1468 c         1981 g         3302 t
ORIGIN      
        1 taccttgtct tatggcgatt acattttgat tgatgagaaa ggaaagaaac gctgatgaaa
       61 acacaacaca aattattgaa tgtaaaagaa gtcatttctg aaaaagtagc agaagcttta
      121 cttattccgc ggtactggac gatagaaacc cctttagtag aggacgcacc gactatgcta
      181 tttgaagacc tagtgaagat tgcaggatat gaactgcatt atcaagaaaa cttggaaact
      241 tatgaaaacg aatattggtt aaccaaatta gagaactaaa aaggagaaaa aatatggctc
      301 agaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa aaaatggagg
      361 atatgacgtt cctataccat gaaacacgtg tacccaaaaa acattacaag gaaaaattgt
      421 cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt gcgacgtact
      481 atcgaacatt ggaagaactg aaaaaacaaa atggaaaatg gtttttccaa gccttactat
      541 gcttggaagc aggcgtcaaa ccaagcacaa tcaaaccttc tgaataccaa gctttagaac
      601 taacgtatgg aaaatttatc gaaacgaaaa aagccaagac tgtttcaagt gaatggttgg
      661 actactttga aaatgtcaac aagtatggcg catgttacat catgaaaaag gaggacaacg
      721 aaaatgagta atgtggttta tcaactaaca gtggaagatg tttctcactt tttaacaaac
      781 aaagaacatt tgaatgaagt agtcagacgg attcaaaaag actttagttg tatggaagaa
      841 atgaagtact ttattcaaag agtggaagaa cagttgtata tggaaagcga aacgagtcaa
      901 acgaaggtca cagtcagcca taatcattta gacgtgtata aacgtcgtcc tgaaacggta
      961 aaaaaaggaa cagagcttct acgactagaa acagaaaaat acatcatgaa tgtgtaccta
     1021 gaaaggagtg agctggtcga aaactttgtc agctacacga tttgtaggaa acactattac
     1081 tcagaatcag aatattattc aatttttgag agcatggaat taatggaaga agaaatggaa
     1141 gagctaaaca gcaaaaatgc gacggtagac gattattacc ttgtatttac tagatttctg
     1201 acgtgtggga cggaatctat cgtgttaatg ggaatggatt atgaactcgc tgtacccgaa
     1261 gcatttttga gggaatatgt agaagaagta gataacccag gcgacgactg gtacacgact
     1321 ttaactgatt ttttagatac ctacacttac gacgatacag aagaaatcca aaatgagctt
     1381 gactgtaaac gaatgccata tcaacgattt gaaaggaaaa aaggtcaata accatttata
     1441 ccgaaaagtg agaatgtgtg aaataagaac aaaaaacctt gaaattaagg gaatggttct
     1501 tatttcgcac caatcgctcc aaccgccccc gccgctcagg tggtgcgaaa taagcgggca
     1561 acgaccgata acctaagaga aagaaggaaa tccatatgaa taaaatcatt cctctgttgt
     1621 tcgtactgtt tgttggcgag atacttatgc gtcatgctgc agctgtacat ttaaacttcg
     1681 tagtattctt aggaatactg gtattttgtt tgatcttttg gcgatttgta ttagcccttg
     1741 tgagtctact attcaagatg attgcagcca tatttttcat agggttattt cgatgaaagg
     1801 agagagtact aatgccaatt gcaataggaa ataaacgttt acccgtgacg ttggatgaaa
     1861 agagacaaaa agaattgcag gaactcaaac agaagtacgg caaaagtgaa tccaggatta
     1921 tgtgtattgc gttagattta ttgattgcac aagaaaaagc agggtttgat gtaccagcac
     1981 tcaaaaagtg acgtcacttt tcatttgaag aaacaaaagt gatagcactt tacgcacgtg
     2041 tcgatacgtg ttatgattat tttgtaagga ggtatagtgc acaatgccac ttacgggaaa
     2101 agaaatgctt aagttattga aaaaaaatgg ctgggttgag cgaaggcaag agggatctca
     2161 tcatcatctt tataaggatg gtgttagaat cactgttcct gttcatgcca atcaagatct
     2221 tggccgagga cttgagcaaa aaattttaaa ggatgcggga ttgaaataat ctcgcattcg
     2281 attaaagaaa ggattgactg aaatgttaag tgagccttta gcaaaaacct atcctgctat
     2341 ttttagccca gaagaaggtg gtgggtactt tattgaattt cctgatgttc aaggtgctta
     2401 cacaggaatt aacgaagacg atatatctta tggaattgct atggcacaag aggtgttagg
     2461 tatggtttta gcagattata ttgagcatga agatctactt ccagaaccaa caccaatcaa
     2521 taagatttct gttgaagatg attcttttac tactttaatt cgtgtagatg ttgccaaata
     2581 tttgaaagac acagaacttg tcaaaaaaac cttaacaatt cctcaatggg cggataagct
     2641 aggcaaaagg gctggcataa atttttcagt ccttctaaca gaatcaattg cagataaagc
     2701 agataacatt cttcgttctg gaagaaataa ttagaactca aaagagtcag aaacttaaaa
     2761 atctttggct tcttttttta ctacaaagat ggcttcttta aattatttgc ctgattgatt
     2821 taaaaaaatt gcgcaaagac tacgagctaa ttaagttgct cgtggtcttt ctttatgaaa
     2881 actttttaga tctagataaa aacattgaga cactttgaaa aattttttta atagtaatgg
     2941 gcgcttctat gtcaagctga ttcttctttc ggtaaattaa ttttacttag aatatcacta
     3001 tcttgataaa aaatggtgta ttcacaaatt ctggcataaa aaatacctac tttctagtta
     3061 aggttcctga ataagtcgca atccaattat ttccagctga tttataactt tttaaatata
     3121 attttccata ataccaataa ccattacttt tttctgtcca atttcgttat tgagaagatc
     3181 tagaaggcca gccaacttta tcaaaaatca tcattcctgt cactcgtatc gttttagaag
     3241 ctgctcgagt aagtaatgga gaaactgtta aataatttta agtaacagtt aagtcgttag
     3301 ttgggttatc aatggctatt ccgtccttat ctgaacctcg aaacccaatc atagaaaaag
     3361 ttttttgaac aagttttaac agataaatca agttatatac tagaaaattc gtaaaatata
     3421 tttttaatgt cataatcgtt tgatttttct ggattgacat ttatttataa aaaatagtgc
     3481 cattaaaaaa gaaaagtgac acaccaataa aagtcatatc ctgtgacatt atatggcatt
     3541 atccaaatag aataaccggt tgacatgtaa gcggattcct tttataattt taggagtata
     3601 agtccttttc taaaaatatt aaccggttat catgaaaggg gatacaaata tatcgttgga
     3661 ggataaaaat atgtcaaagg ttaacgtatt taaaaatcaa tcctatttac aaagttcagc
     3721 tacattatta ctattttttg cttcttgggg tgtttggtgg tcattttttc aactttggct
     3781 aacatctgaa tcaaatggtt tagggttatc tggcagtgct gtaggaacag tattctcggc
     3841 aaattcgtta gttaccttaa ttttgatgtt tatttatgga acattacaag ataaattgta
     3901 tattaaacga aatttattaa tttttgcttc tgtattagcg acacttgttg gaccattttt
     3961 tatatggata tacgggccat tgctagataa caattttaat ttaggcatta ttatgggagc
     4021 actatttttg tcagctggat atttagcttc tgtaggagtt tttgaagctg tgtcagaaag
     4081 gtttagtcgt ttatttggct ttgaatatgg acaagcaagg gcgtggggat catttggtta
     4141 tgccttggta gcgcttttgg caggattttt atttgtaaaa aatcctcatt taaacttttg
     4201 ggcgggatct ttctttggtt ctttactatt gttaaattta ttattttgga accctaaagt
     4261 tgaacgggaa gcaaatcaaa attttaatca agaacaagct gaatcaaata gtattccttc
     4321 tttaaaagaa atgtttgatc taatgaaact gcctcaatta tggacgataa tcatctttat
     4381 tgtttttaca tggacatttt atacggtatt cgatcaacaa atgtttccgg gattttatac
     4441 tggtttgttt tcaacatcag ctaatggtga aaaaatatat gggacattga atgctattca
     4501 agtattttgt gaagcgttaa tgatgggaat tgttccaatc attatgagaa aattaggggt
     4561 tcgaaatact ttgttattag gtgtaaccat tatgtgtgta cgaattggat tgtgcgggtt
     4621 tgcctcgaca ccattatctg tttcatgcat aaaaatgttg catgctttag aagtaccatt
     4681 atttacatta ccaatgtttc gctattttac acttcatttt gatacaaagc tatcagcaac
     4741 cctctatatg ataggatttc agatagctgc tcaaattggg caagtgattt tatcaacacc
     4801 attgggaata ttaagagaca acgttggcta tcaaccaaca tttaaaatta tttctcttat
     4861 tgtattacta gcaggcatat atgcattctt tattcttaaa caagatgata gagatgttca
     4921 aggggatcca tttattcgag gataatggaa accaacagaa taaaaaacaa ttttatctag
     4981 aaagtgagcg aatatattat gacaaaaaga gctatagaat taacaaatac acgttatcgt
     5041 ttgggatatc atgtgtctgc accatcagga tggattaatg atccaaatgg attttgctat
     5101 tttgatgggt attatcatgt gttttatcaa catcatccat atagtgctga atggggacca
     5161 atgcactggg cacatgctag aagtaaggat ttagttcatt gggaatcttt accacttgct
     5221 ttgactcctg gagatcaaga agatgaaggt ggttgttttt caggaagcgc tattgaaaaa
     5281 aatggtgtgt tgtatctgtt ctatacagga catcactatt atggagacaa tgatccagat
     5341 catttttggc aaaatcaaaa catggcgtat agtacggatg gcattcactt tactaaatat
     5401 gaaaaaaatc ctgtaatagc aaaagcacca gaagataaca cacatcattt cagagatcca
     5461 aaagtatgga aacacgatga aaaatattac atggttttgg gtagtcaaga aaaagatgga
     5521 ttaggtcgtg ctattgttta tcagtcgtta gatttattaa attgggatta cattggacca
     5581 atttctaaag cgaaggaagt acttacagaa ggatttatgt gggaatgccc agactttttt
     5641 gaattaaatg gaaaatctat tcttttatta tcccctcagg gaattgaagc aaatggaaaa
     5701 gattatttaa atctatatga aacaggctac tttatcggtg attatgatta tgagacagca
     5761 aaattcatta gaggaaattt ttatgaatta gataagggac acgatttcta tgcaacacaa
     5821 acaactttat ctccagatgg tagacgtatt gttatggctt ggatggatat gtgggaaagc
     5881 cctatgcctg aaaaagtaga cggatgggca ggagcattaa cgcttcctcg agaattagag
     5941 ttaattggag atcatttgta tatgaaacct gtgagagaat tagaacaact tcgagttggc
     6001 agcggcgttg aaacaaaagt taatttggca ggagaaataa taatcgctca agatgcttca
     6061 ttcaaagaag ctctaattga tgtactattg actaaatcaa aaaatcaaga aatcaaattt
     6121 tcattaaaaa caaaagatga agagttaatt aatatttttt attctaagga taaaaatgaa
     6181 tgtaccttaa ctcgtaacgg caaaaatgat ccaaggtatg gaaccattca gatgtgtgat
     6241 agattatcac ttcgagtgtt tatcgataaa agttcggcag agatttttat taatgatggc
     6301 gagcttgttt ttacagagcg ttattataca gaaaatcaac caagtatgca tttgagtgtt
     6361 agtgaagaag cttttgtctc gagtacaatt tatcatctaa aaaaagatgc aatttcttac
     6421 gagtaaggag gttttatgat gttaattggt agcatagaag caggtgggac taaatttgta
     6481 tgtgccgtgg gagacgagga ttaccgagta caagaccaaa taatttgtcc aacgaccacc
     6541 cccagggaaa cattgaaaaa aactgttgac tattttaaac agtttgatat aaatgcatta
     6601 ggtattgcat catttggtcc aatagaaatt cgaaaaaatt caccaaaatc aggatatatt
     6661 acgtctacac caaaggtggg atggcgaaat gttaatgtgt taggttattt acaagatgag
     6721 ctgaatgttc caatgtcttg gacaacggat gtgaacggat cagcttatgg agagtacatc
     6781 atgtccactt tatcgaatga aaaaataaat tcattagttt attacaccgt tggtactggc
     6841 gttggtggtg gagctgttgt tgacggaaaa ctattaggat cgtttggtca ttcagaaatg
     6901 ggccatacgt ttgtaaaacg ccatcctgat gatattgatt ttaaaggaat ttgtccttat
     6961 catggtgatt gtttagaagg cttagttgcg ggaccaacgt ttgaagcacg ttttggaaaa
     7021 aagggtgaag acgtttcttt aacggatcct atttgggatg taatggccta ttatctagca
     7081 caagctgtga ttcaaacaac attgattata cgaccggata aaatagtatt tggtggaagc
     7141 gtagtaaatg aaaaattgtt agaaaaaatt cgtgttaatg ttaatgaatt attggatgat
     7201 tacgtggaaa taccaccgtt aagtaaatat attacgcgtc ctttaattaa aaataatgga
     7261 tcggcaacac taggaaactt tgctttgggc ttgaatcaat tacaagaata aaacatagaa
     7321 aaaatatcga ttatcaggta tagtagttct atagagttct ttttttcgat agttaattat
     7381 tgcattattt aggagagaca agagaatgcg cccaaaatta gaagatgttg ccaaaagagc
     7441 aaatgtatca aaaacaactg tttcacgtgt attaaacaat cgtggctatt taagtcaaaa
     7501 aacgattgat aatgtttata aggctataga agaactcaat tatcaaccga acgttgtagc
     7561 gcgtcaatta tttcaaaaga aaactaacat tgtcgggtta ctatttccta cagtagccaa
     7621 tccttttttt agtgaactcg ttgaggcatt ggaaaaaaag ttatatgaaa ttggttataa
     7681 ggtattaata ggcaattcga tgaataataa agaaaaggaa acaaattatt taaatcagtt
     7741 attgtctgat caagtagatg gtttaattgt tggtacacat aaccaaggga ttcaggaata
     7801 taaatatcag aatttaccga ttgttgcaat tgatcgagtg atgaatgaag atattccagt
     7861 tgttgaatcc gataactaca atggtggaaa acttgcaacc aagttattaa ttgcacaagg
     7921 tgcaaaaaat attattcata caaacggtcc aattgactta cagactccgg ctaatcgtcg
     7981 gagactagcc tatgaagata ctatgaaagc atatcagtta attccacgaa ctgtgacatt
     8041 agatttcaat atttcttatg taaagaaaaa acagattttt ttccaaatgt ttgaggatta
     8101 tcctaaaata gatggtattt ttgcttcaaa tgacatcgat gcggccctaa tattacaagt
     8161 agcaaaagaa aaggggttaa atgtaccagc tgatttatta gtagtcggat atgatggtac
     8221 tttaatgact cggagtatat taccagatct gacaacagta atacaaccaa ttaatgatat
     8281 agcagacaca gctgttgcta ttttgatgaa acgaataaat aaagaagaaa caaaaaagga
     8341 atatatttta cctgtcacat tatggatggg gaagacatca gtaaagaaaa aggacaatta
     8401 aatttataaa gactatcaat gataatttca ttattaattc gtgatataaa aagttgttaa
     8461 atctcttata atagactatg atacaaaatc atgcttttaa tctgtgtaga tgttgccaaa
     8521 tatttgaaag acatagaact tgtcagaaaa accttaacaa ttcctcaatg ggcggataag
     8581 ctaggcaaaa gggctggcat aaacttttca gtccttctaa cagaatcaat tgcagataaa
     8641 gcagataaca ttcttctttc tggaagaaat aattagaact caaaagggtc agaaacttaa
     8701 aagtttttga tcctttattt tcaagtctga tttgacagat tttcggcagc tcatttattt
     8761 atggtcagaa atagttgctg aaattttaat ctattagcaa atagtccaga acgtatgaag
     8821 aggattcgga tatcatttgt cattaacacc ataaatattt ataaaattcg tgttcaccta
     8881 caattaaatt taatatataa tattcgttgt aagggcttat aattttttgt taatgaacaa
     8941 gatatgtaat tgatagtgtc aatagctatt caaaaatgta ggcttttggc ccataaataa
     9001 caaggttact tatatgataa ctttttttct ttataatctt taatccgata gagctttcca
     9061 gtagtttgaa taatcttagc gtggtgcact aatcgattga ttagtgcatt tgtgttcagt
     9121 ccattgagac ataatagtgg atagttttac tgagaaaatt gataaaaaat agagatataa
     9181 ttcaaatacg tatataatga aactaaagat aaatacggag gaaaatatat gattataaaa
     9241 aacttaggaa atgttaaatc aatagatata cacgttaaag atttgctgat ttttactggg
     9301 aaaaataata cgggaaaaac atataccagc tatattttat atggaatttt atcagaactc
     9361 tttggagcaa gatttttcaa ttatttttct catacagaaa ttgaaccaca tttacaaaat
     9421 agtttaaata ttttattagt tagcaaagac tctataagga aaaaatttaa taatgatgca
     9481 aaaaaatttt tagaagaaaa tttaaaaaaa attgcagttc gtaactttaa agtaagtgaa
     9541 aataacttca aaaatctttc ttttactatt tcagattcag aaattagtga gctcactggt
     9601 gatatcttta attatgagat caaaagatac agtataagtg gcattgttat aacaattcaa
     9661 aatgtaggag agaaattaaa aattagcttt gagccaactg attcatttca gtatcttagc
     9721 aaagatattg aaattgaaga tatttataaa attttagctc aagagctatc taaaaatgtt
     9781 attagtttgc caaaggtttt ttattttcca gccgaaagaa ctggtataaa tgtctttaag
     9841 aacgaactaa atgaaagtcg cttaaaaaca tatgatacct tgctgaatac tgtgcaattt
     9901 actaatttaa ctaaccaaaa aaagaaagct gccctcaagt acgatctgtt aaaaaagaat
     9961 attgagctgt taatggagac aacggataat agttcttatc caaagccaat atcggattat
    10021 attaattatc tgaataacat gaaaaataga tatgaacaga aaaatgtcaa taatatagca
    10081 tcatatatta gagatgagat tattatgggt aaatatgaaa ttgacgaaaa agataattct
    10141 gtaagtttta gacaaaagtt tggtaaaaat agatatagaa aagaacgaat tccttttcac
    10201 gttgtgtctt cttcgattaa atctctatat ggattagatt attacttaga aaattcaagt
    10261 aaagaaggag actatcttat tattgatgag ccagaattga gtttacatcc agaaaatcag
    10321 gttaagttag caaaagtaat tacagatata gttgagagta aaattaagct aattacttct
    10381 acccatagtg atttatttgt acgtgagctt acaaatattg ttattgaaaa taaattaaat
    10441 agtaagaata ataaaaaaaa taaattgtct gataaaaata taggcatata taattttgta
    10501 ggaaataatg atataaatga attagaaaaa attt
//
LOCUS       DACCKU010000094        10502 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.097, whole genome shotgun sequence.
ACCESSION   DACCKU010000094 DACCKU010000000
VERSION     DACCKU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.097"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(212..964)
                     /gene="xth"
                     /locus_tag="IVN13_002373"
     CDS             complement(212..964)
                     /gene="xth"
                     /locus_tag="IVN13_002373"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP7012828.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1077..1997
                     /locus_tag="IVN13_002374"
     CDS             1077..1997
                     /locus_tag="IVN13_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP7012829.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2070..3005
                     /gene="murB"
                     /locus_tag="IVN13_002375"
     CDS             2070..3005
                     /gene="murB"
                     /locus_tag="IVN13_002375"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP7012830.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3303..4781
                     /locus_tag="IVN13_002376"
     CDS             3303..4781
                     /locus_tag="IVN13_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7012831.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5058..7118
                     /locus_tag="IVN13_002377"
     CDS             5058..7118
                     /locus_tag="IVN13_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012832.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7201..7770
                     /locus_tag="IVN13_002378"
     CDS             7201..7770
                     /locus_tag="IVN13_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012833.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7945..8751)
                     /locus_tag="IVN13_002379"
     CDS             complement(7945..8751)
                     /locus_tag="IVN13_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012834.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8889..10403
                     /locus_tag="IVN13_002380"
     CDS             8889..10403
                     /locus_tag="IVN13_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP7012835.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3725 a         1750 c         2068 g         2959 t
ORIGIN      
        1 taataagccc aaccaaacga aaaatatgag gaactatttt tgtttggctg ttcttattac
       61 ttgatgcagg acagcttttt cacaatctct cgataaacag tgttcaaaag ccagtttctc
      121 ggtattatgc caaatttatg caaaatgtgg aaagccattt tcacaaaatt tttcttaata
      181 ctcgaaacta aacgactttt tcacaacctc tttatttcaa atctaattct actggacaat
      241 gatcactgcc cataatgtcc gtatggatct ttgcatcagc tagtcctggc tctaaatctt
      301 tgctcgtcaa aaagtaatcg atccgccaac cagcattgtt tttccttgaa ttgaatcggt
      361 agttccacca agagtagaca ccttcctggg ttggataaaa ataacggaat gtatcgataa
      421 acccgttatc caacagtcga gaaagagccg ctctttcttc tggagtgaat ccagcatttt
      481 tttgattcgt tttccagttt ttcaaatcaa tcttttgatg agcgacattt agatccccac
      541 acacgatgac tggtttttgt ttttttagat tttccaaata attataaaag gcttcttccc
      601 attctaatcg gtaatctaaa cgttttaatt cgctttgaga atttggcgtg taacaggtta
      661 caagaaagaa atcggaatat tccaaagtaa tcagacggcc ttctgtatca tggatatcga
      721 tacccattcc gtaagtggca ttcaatgctg gttttttagc aaaaatcgct gtgccggaat
      781 agccttttcg ttccgcgtaa ttccaatatt gataataacc tggaagatct aaatcaattt
      841 gtcctgcttg gagtttcgtt tcctgcagac agaaaaaatc ggcgtctaac tcatgaaaaa
      901 cttctaaaaa atttttattt acgatggcac gaagcccgtt tacgttccaa gagataaatt
      961 tcaataaaag cacactcctt cgaattaatt tccttacctt tgcgatacta ttgtacctca
     1021 ttttagtaaa aaatggtgta agatgaaagt aactaaatga tccaggagga aagtagatga
     1081 agattggtgt aatcggtgta gggaatatcg cagaaaaagc atatttacca acttatgcga
     1141 aaaaacaagg aacacttgat ttttattttg caacaagaaa taaagaaaca aaagaaagaa
     1201 ttcaaaaaga gtacgggttc caacatctat atgaaacgat cgatgagctt ttagaagaaa
     1261 aaatcgaagc atgtatgatc cacgcagcaa caaaagtaca ttatgaatta gccaaaaaat
     1321 gtctagaaaa tggtatccat gtttatattg acaaaccact gagtgtccat ctaaatgaag
     1381 ttcgtgaatt gcaagaatta gctgataaga accaaataat tttgatgatc ggtttcaacc
     1441 ggcgctttgc tcctatggta gaagaattga aacgtatccc agataagcgg ttgatccacc
     1501 tgcaaaaaaa tcgaatagcg gcaaaagaaa cgacagagtt cgtcctttat gatttgtttt
     1561 tacatcttat tgatacagct gtgtatttat tagatgaacc cattttgaga acaaaatacc
     1621 agataagaga aacaaaggaa tttttggaat atgccatttt acaattagaa acacaaaatc
     1681 aaacggctat tttaacgatg gatttagcaa gtggcgctaa tatagaaaaa tatcaagtaa
     1741 caagtaaaaa tggtacttat gaagtaaacg atctgaccca gcttgtcatt caagatgctg
     1801 atggaaaaaa agtgaaagaa tttggcgact ggacaaacac gctagtcaaa cgcgggtttg
     1861 agccaatggt agaggccttc ttttctcaga tccgtttagg taaatcggaa acagaattgc
     1921 ttaaacaaaa agaagttgta agaagtcatg aattatgtga ggaagtgctt aaagatcact
     1981 accgtcatca actttgaaca agactggcaa gattgtatta agcgtgatag aataatgagg
     2041 acgtttgaga aaagaaagga aaatcctcag tgaataaaga agaaatagtt gaaagcttag
     2101 ccacgattac tatattgttt gatgaaccat tgatgaacta tacgtttacg aaaacaggtg
     2161 gaccagctga tgtccttgca ttcccgaaaa aacaagaaga agtaaagcaa atcatcgatt
     2221 attgccgaat ccatgatata ccttggatgg tattaggaaa tgcaagcaat ctgatcgttc
     2281 aagatggcgg gattcgcggt gtagtcatca tgctgactga aatgaaacag attcatgtca
     2341 aaggaacgat ggtcattgcg gaagcaggtg cctcactgat tgatacgact tatgcagctt
     2401 tggcagaatc attgactgga tttgaatttg cttgcggcat cccgggaagt gtcggtggag
     2461 cggtttatat gaatgcaggt gcgtatggcg gagagatcaa agatgttttt gctgaagtgg
     2521 atcttttgct tgaagatgga acattgaaaa ctttgacaaa agacgaaatg gtattttctt
     2581 atcgtcacag taaagtccaa gaattacgtg ctatcgtatt ggaagctcga ttcagtttgc
     2641 aaacagggga ttatgaagca atcaaagcaa gaatggatga gttgaccgaa ttgcgtcaat
     2701 caaaacagcc attagaatac ccttcatgcg gaagtgtctt taagcgccca gtaggacact
     2761 atacaggtca gctgatccaa caagcaggat tgcaaggcct gaaatggggc ggtgcacaag
     2821 tctctgaaaa acatgctgga ttcatcgtca atatcgatca tgcgacagcg acagattata
     2881 ttgaattgat tgcgcacatc caagaagtaa tcaaagaaaa atttgatgtg tctttagaaa
     2941 cagaagtgcg gatcattgga gaaaaacaag caatatcaga aacaagcaga agtttagcca
     3001 attaataagt aatggaactt aaaataagta aaagagcagg acggtggccg tcctgctctt
     3061 ttttgtggtt gtttacgcaa atggcgcaaa tcttgtacat tttaacacaa taaaacaaaa
     3121 aatatagaat atttcctcat aaatttttcc attttttgca atattctcaa aaaaaagata
     3181 aaaaatgaga ataactgatg aaaaaacaag cataatccat cataaaatca gtataggtga
     3241 aaagatgaac tttcatctgc atatcaagta attggtatga ggcatttttt gaggtgtagg
     3301 atatgaagtt ggattacctt atagaagagg aagatagaga aaaactgctg ctagctagat
     3361 atttagaatt tgagaaaaag aattatgtag tgctgcctaa gttatatgaa acattaggtt
     3421 tgtctaaatt taaaacaaga aattatatca tagaattaaa taaagaattt actgaattca
     3481 aaacaaagcc agagatccaa atttcagaag aagcagagat ccatgtcgaa cgtctgaatt
     3541 tgacggatat caaaaattta agaagaatct actttcaacg atcaaaaatt gctcgactgt
     3601 taacggagat cgtggattca aaagtaacga tcgaaaaatt tgcagagaat cattttttaa
     3661 gcatatcaag agcctatgta aaaagaagag aattagtctc ttttcttgaa cagcatcaaa
     3721 taaagttaaa gaaaaacata ttagttgggg aagaaataaa catacggaac gtattatttg
     3781 gggtatattt cggggtcttt aatggttttt ctatgccatt tagagaaagc gtcatgaatg
     3841 aagcaaagag tatcgtgcaa tattttgaat atctgttcaa tttaaaactg tctgcaacaa
     3901 aacgaatcaa attcaatttg ctaaccacta ttagtttgtt gagggcctat gggaatcaac
     3961 caatcggaaa acgtttcttt tcagatgaat actttaagag ctcagaagga atgaagatca
     4021 tcaaggattt atcacatatt tcttttcttc atatccctaa agagcagatc attgatgaga
     4081 tttcttatat catgttgttt ttgatcacgg aagaagaatt tgaattagat ggcgcatttg
     4141 gacttgcgga agctcatttt catgatatcc acatgattag tcaaaaaatg tcagtggaat
     4201 tgattgatca attaccattt tcgattgaaa tctctgaaga agaaagacta tatatacgaa
     4261 aagaacttcg tatgggtatg atgcatgtca atcgaaaaaa ttggatattc gactttgaga
     4321 tgactagctt tacgtctaaa aagcagctac agtttttctt cgagacgtat ccaatattca
     4381 gtacagcaat ccaaaaggtc atacaagaga ataaacgcta tttcttggga aaagatgaac
     4441 gtttcatgat tcgcttattt tatgattatc tctttttatt ggtcggtatt tgtccggtat
     4501 catattttga agcaccgatt catgtctgta ttgatttttc gcaaggatct gcttataccg
     4561 attacattat ttctcaagtg aaaggattta aaaattacaa tattgtgatt gagagtcgaa
     4621 tcacttctcg tacgaatatt tatttatctg attgtttact tgaaaaattt aataaagaac
     4681 agataatttg gaaaaatcca ccgactccag atgactggga atattttgga aataccatca
     4741 tcagagaaaa aaatagacat ttaactgaaa aaatcagcta gtatttatgg atggcagctt
     4801 tgtctcacat atttattagc tgatttatgt ttttttcaaa gaaaaggtaa ctagatataa
     4861 ctagatataa ctagatatat tacttaacaa aattaaaaag tacataagtt attattccga
     4921 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
     4981 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
     5041 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
     5101 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
     5161 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
     5221 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
     5281 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
     5341 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
     5401 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
     5461 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
     5521 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
     5581 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
     5641 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
     5701 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
     5761 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
     5821 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
     5881 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     5941 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     6001 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     6061 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     6121 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     6181 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     6241 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     6301 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     6361 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     6421 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     6481 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     6541 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     6601 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacaagaag
     6661 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     6721 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     6781 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     6841 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     6901 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     6961 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     7021 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     7081 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     7141 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     7201 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     7261 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     7321 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     7381 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     7441 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     7501 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     7561 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     7621 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     7681 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     7741 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
     7801 gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata
     7861 actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc
     7921 ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat
     7981 gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac
     8041 tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta
     8101 accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt
     8161 ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt
     8221 gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa
     8281 ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag
     8341 taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa
     8401 gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa
     8461 atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa
     8521 aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat
     8581 ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac
     8641 ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat
     8701 tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact
     8761 ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta
     8821 aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg
     8881 ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga
     8941 gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg
     9001 ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat
     9061 cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa
     9121 aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat
     9181 ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca
     9241 aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata
     9301 aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa
     9361 acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc
     9421 gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc
     9481 atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg
     9541 aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa
     9601 aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc
     9661 gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca
     9721 ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa
     9781 ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct
     9841 tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg
     9901 cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg
     9961 atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa
    10021 tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg
    10081 gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg
    10141 aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca
    10201 cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa
    10261 ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg
    10321 gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc
    10381 gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg
    10441 ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa
    10501 ag
//
LOCUS       DACCKU010000095        10491 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.098, whole genome shotgun sequence.
ACCESSION   DACCKU010000095 DACCKU010000000
VERSION     DACCKU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10491
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.098"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            145..363
                     /locus_tag="IVN13_002381"
     CDS             145..363
                     /locus_tag="IVN13_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012836.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(652..1164)
                     /locus_tag="IVN13_002382"
     CDS             complement(652..1164)
                     /locus_tag="IVN13_002382"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP7012837.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(1178..3475)
                     /gene="recJ"
                     /locus_tag="IVN13_002383"
     CDS             complement(1178..3475)
                     /gene="recJ"
                     /locus_tag="IVN13_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP7012838.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3617..4051)
                     /locus_tag="IVN13_002384"
     CDS             complement(3617..4051)
                     /locus_tag="IVN13_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP7012839.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(4061..4849)
                     /locus_tag="IVN13_002385"
     CDS             complement(4061..4849)
                     /locus_tag="IVN13_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7012840.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4846..5787)
                     /locus_tag="IVN13_002386"
     CDS             complement(4846..5787)
                     /locus_tag="IVN13_002386"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP7012841.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5964..6551)
                     /locus_tag="IVN13_002387"
     CDS             complement(5964..6551)
                     /locus_tag="IVN13_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP7012842.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6772..8085)
                     /gene="obgE"
                     /locus_tag="IVN13_002388"
     CDS             complement(6772..8085)
                     /gene="obgE"
                     /locus_tag="IVN13_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP7012843.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            8373..9383
                     /gene="gap"
                     /locus_tag="IVN13_002389"
     CDS             8373..9383
                     /gene="gap"
                     /locus_tag="IVN13_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7012844.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9468..10403)
                     /locus_tag="IVN13_002390"
     CDS             complement(9468..10403)
                     /locus_tag="IVN13_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7012845.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2896 a         2369 c         1818 g         3408 t
ORIGIN      
        1 tttgagacgt tggtctcaaa ctcttttttc gcccgggttc ttcttctttc aaatttttat
       61 cacttccctc tttacaaacc gaacaaatat tcgtatacta aatatacgaa caattgttcg
      121 cataaattaa agaggtgaga attcatgaaa tcaatgagac gtcttggctt agtattaact
      181 gttttgtttt taggtattat tattggtaat ttcggcatac gtccagcttt acttgtgttt
      241 gttccgttat ttactttgtg gttcatgttg tgggatgaaa agaagtaccg tcaatctgag
      301 cggcgcagaa ttcatgaaga tcatttctat catgagccct actacagatc caggcaatca
      361 taataaggat tgactgctaa ttattcaaaa gacaagaagt taaactttta cgggggtgtg
      421 acaaaaatcg gtcacatcct ttttttcgaa taaacggtgt tctaaagcca gtttttcggt
      481 attaagtcaa attgcggaaa aagtgatttt ttaaacttct actggtcttt gcttgtttcc
      541 atttataaaa aaatagcgac cctcaaaagt cagatagaga atctgacttt tgagggtcgc
      601 tttaaaatcg cttctttaag aataacatca agaatagctt cttttttctg cttaatattc
      661 cattaatgta atgatgtcat atcctttgat tttgtcgcgt ccatggagat ccatcaattc
      721 gatcaaaaat gcgcagccta cgactacacc gcctaattgt tcgatcagtt cgatcgtcgc
      781 tttgattgtt cctccagtag ctagcaaatc gtcacaaatc agcacacgtt gacctggttt
      841 aatggcatcc ttatgcaatg tcaatgtatc tgttccatat tctaatccat aagtcacttc
      901 gatggtttct cgaggtaact tccctttttt gcggactggg gcaaaaccga ctcctaactc
      961 ataagcaaca ggacatccta cgatgaatcc acgggcttct ggcccaacta ccatatcaat
     1021 tcgtttttct tttgcataat ttacgatttg tttagtagct tcccgataag cgtcaccatc
     1081 tgccatcagt ggggaaatat ctcgaaacac aattcctttt gacggatagt cgggaatact
     1141 ggcaatataa tcttttaaat ccactctttg tttcctccta ggttgttaac catttcttta
     1201 tggtaggaat atcgctcaaa agcaaaaatt cctcaacttt gattttattg agccgtcgct
     1261 ggtataatct gctctcagtc aaaggatgac tttctgggct agcgactttt tgtaaaacac
     1321 cgttctctat tgtaacaaat ttcaattcaa aaaacacctg tatcataaaa attaataatt
     1381 tttcagggat acgcaaatat tgagctattg cttttatttt atgacgtata tcaattcgtt
     1441 cttgagaatg gatcaattta aacaattttg cgtactgttc tcttgtgcct accccattca
     1501 aataggcctc atcaggtgaa atccccatca gataaacacg attgaatgaa taagaccgaa
     1561 aaacttcttt cagatccgct aaatcatctg gacaatccat aacaactaga gagtttggtt
     1621 ttcttttttc aattattgcc tttacagaat ctagtccttc gaaaacaatc gcagcttcct
     1681 ggataatctc aggcacgaat ttcaacgtcg aagaatcaaa tgctaaataa aggctacctt
     1741 ccgacgttcg gctctgttca cggaaacgtt ttgcccgcca atcaaatact tgcagaccac
     1801 ccacagaaaa atcagatacc atcagctgcg gtttcttacg cccgttccat tcattgatcg
     1861 aaagctttcc taccacatct acctgattat tcagcagttc ttcttcttga gtaccaaaac
     1921 caaaagcgac cgcatcaagc tgactcgatg catcggaaag gacaaatttc agatgctgtt
     1981 ggtttgctcc gattcttttg atattttctg cttggacttg tcgaaataaa aactgaggca
     2041 aaggattatc cgtaccaaat ggcgccaata gtttcagctc atcaattcgc tctactgtta
     2101 cttcatttgg cagcaatact tcatcaattc tcaactcagg ccctctcatc aagtcaattt
     2161 gatgatcgac tatatattgg ttcatcttct cctgtagtat agtgacattt tcacttggca
     2221 tcgttaaacc gactgctgca tgatgtcctc cgaagtaagt aaataaatct cgcatctggt
     2281 caagcatttc aaaaagattc aaggcttcaa tactacgtcc agatccttta gctgatccat
     2341 cttcttttaa agtaaggaca agcgttggtt tacccgtttc attcattatt ttcccagcaa
     2401 caatgcccaa aacgccttca tgccatcctc ggtttaccaa tagatggata tgattctcct
     2461 tattgaccat cgctaatgct tcttgtgtta tctgctctac gattgctttt ctttcttcat
     2521 tgatctcatt caattttttt gcttgaacgg atgcttcctc ttcatcaaac gtcgcaagta
     2581 acgagacggc aggacgtgga tcgcccatcc gaccgattgc atttagtctg ggagcaatcg
     2641 aaaaaccgat cgttgtttcg tctgcgtccc tcatttttac gccgctttct tcaaatagcg
     2701 cctgaagtcc tatccgttct gaatgatgaa tggcatcgat tcctaatgaa acaaggattc
     2761 gattctcatc agtcaatgaa actaaatcgg cgatcgttcc tatcgctact aaatccaaaa
     2821 attctgcagg cggttcttcc aataatgcag aagctacttt gaatgctact ccgactcctg
     2881 ccaaatctgg aaatggatag tgccccgctg gatgacgagg gtgaacaatc gcataagcgt
     2941 ctggtaaatt ctcaggtagt tcgtgatgat ctgtaatgat cacgtctaca ccagcttgct
     3001 gagcgtatgc taccgcatca tttcctgcta ccccgttatc aacagtgatg atcaactgga
     3061 ttccttcttc gatcttttct tggtataccg attggtttgg tccataccca tgctcaaatc
     3121 gatttggcag gaatacttct acttctgcac caagtaattc cagtgtttct ttcattaccg
     3181 ttgcactggt gatcccatct gcgtcataat caccgtaaac gaggattttt tctccactga
     3241 ttacagcttc ttggattcgg ctgattgctt tatccatatc gtgtatcaga tacggatcat
     3301 gtaattcttg ttcctttgga tgtaaaaatc ggttgatatc cttttcctca tggtaacctc
     3361 tttgccacag taacttaccg acaaatggag ataatttcag ttcacggatc atttggagaa
     3421 aggattcaga aggctctgtc tcgcttaact tccaatcaaa atttgcttgt ctcactttct
     3481 cactcctaac ttagttatta tagcaaagga aatcagcaaa aaaaagcaga aagaaagaat
     3541 aaaaacagat cagatccgct agacagatgt tcagcaggac ctgatctttt gtacgaccat
     3601 ttatgaaata tccctattag tcaaaataat tgaagctttg tttgggcgat tgagtttttt
     3661 gttcttccgg cgctttcaat tgactgattt gtgcggtttt cgcttgtaac tccgcctgat
     3721 aagcagcttc caactccgca cgttcgtttc taaactcgcg ttgctgttct tcttttgctt
     3781 cttcaatttt cttttgaagt tctgtttgat attctgacat ttcctgagtc agctgtttga
     3841 tttgcttttt ttgctgccat agtgttgccg aagcagtcaa aaatacaatc agtgcaccaa
     3901 tcagcgcaga accgatgatc actaagatca atggcgcaga aaaggaagtg aagccaaaac
     3961 ttacaggaac atttttattg ttcaatacag caaaaagaac aataatcaaa actaaaacaa
     4021 atccaagaat cacattgctt tgctttttca attccttcac ctactttttg ttaaaaattc
     4081 cgctggctaa ataatcacca atcgttggaa acagcgtata aaattttgat gcaacttcta
     4141 aaataaacgg ttgattgatt tcccgttttg gatgtctcat attgcggacg atcatttttg
     4201 ccaacttggc aggttctaaa acgatttgtc cgactttttc caaatagctt ccgcttggat
     4261 cagccttatc gaagaaattt gtctcgattg gacctggatt cactgtcgta acagctacgc
     4321 caagtggttt caattctaat cgcaaagcgt tggagaagcc aaggactgca aatttcgtgg
     4381 cagaataaac cgttgatttt gcagttgcca tttttccagc catagaggcg atattaatga
     4441 tatgaccttg tcttctttct gccatatcga tcgcaaattt ttgtgtcaaa accatcattc
     4501 ctaagatgtt gacttcaaac atatcgtatg cttttccaag gtcgaatgta aggaagtctt
     4561 cgaaaatccc aaaacccgca ttgtttacca gaatatcgac gcgcccgact tcttccatca
     4621 ccttttcata taattcgtcc acgctttcag gattgctgac gtctaccttg aaagcataag
     4681 cctctttgcc gctcaagcgt ctgcactcgt ctctgacacc ttctacaaat gccattcttc
     4741 gtgcgcaact tataacgatt gctccttgct ttgctgcttc ataacagatt tgtgcgccca
     4801 gaccgcctga actacctgtt actaaaacaa ctttatcttc taaattcatg cctcttcctc
     4861 ctcgcgaaat ggaatctcaa tgctatcaaa atctttcata atcgtcgtgt tttcaaacac
     4921 ttcttgtgct tctttttcaa gctctaaagc tgcttttcct aaatatctcg cactgatatg
     4981 agtaagtagc agccgttttg cctgcgcttc ttttgcaact tcagcagctt gatgagtcgt
     5041 ggaatggaaa tagttgtgcg ccattcttgc ttcatcttta ttaaatgtgc tttcatgtac
     5101 cagaatatcg ctattttccg caagcacaac ggaattatgt gtttttctcg tatcgcctaa
     5161 aatagtcacg attcgtcctt ttttatctgg acctacgaaa tcttttccat tgatcgtccg
     5221 cccatcttct agttggatcg tttcaccttg cttcagtttg ccgtacagtg gtccagctgg
     5281 aatacctaat tcttttagac gatccacctg caattctcct ttatgatcat gttcaaccac
     5341 acgataacca aaactgtcga tcccatgatt tagttttttt gtataaacag aaaattgctg
     5401 atcagtaaaa atcggggctg tttcatttag ctcaatgaat ttcagcggat aacttaaacg
     5461 cgtttgcgaa atacctaaag agacttttat gtattcctct atcccttttg gcccgtaaat
     5521 ctctaatggc gtatccccac cttgaaatga ccggctgctg atgagcccgg gaagtccaaa
     5581 aatatgatcg ccgtgtaaat gtgtaatgaa aattttaccg atttttctcg gacgaatagt
     5641 cgttcgcaaa atctgcattt gcgtgccttc gccacagtcg aacaaccaaa cttcattgcg
     5701 ctcgtctaaa agtttcaatg ctatacttgt cacatttcta tgttttgcag gaacacctgc
     5761 acctgttcct aaaaattgta attccataat ctacctgact ttctttaagt tcctctttaa
     5821 ttttagggta aaaagaaggg ataatcaacc gtaagtcgag aatgggttca aattaaaaat
     5881 aacgttctaa agcgatagcg ttttctttga aaattactat aaaaacagca gcgaacccgt
     5941 ttgagatcct ctgctgtttt ttattaattt gaagatccag aactttcatt gtcgttggaa
     6001 gtttgcggat gtttgtcctt ccaagtcttt tgtgcagctt ctttcgcatg attttcagct
     6061 tctttcgcat catgcacatc tttttcgaca tcttcataat ccaagcgagt aatttctccg
     6121 cctaattcca tcattaccaa ttgattcagt gggcgattgt cctcttcatc agcaatcaag
     6181 atcagtcctg tagctccttc accgatttta tttcctacaa agtcgaacac ggtctgggca
     6241 tcttttattt ctttggcatc ttgtgtcgca ccgatcattc ctccggcaaa ccatcctaat
     6301 aaaatcccta gcggaccacc taatacacca accaacattc cgattaatcc acccgtagaa
     6361 gatttgttat taccggtaaa gtcgataaaa tcttctattt tgaattgatg tgtcccatta
     6421 ggatcatggc tgacaactgc catctgttct cctttaatcg aacgaacagc atgtaacttt
     6481 ttgatttctg aaaatgcctg atatgcctgg ctttctactt gaaaatttaa aatgattact
     6541 cgtttgctca ttaaaaacgc ccctttcata ccttcttgtt atttatttta cctttaattg
     6601 aaaaataaga aaagaaaagc actctttttt tccaataaaa tagggctgtg acaaaagtcg
     6661 tatcacagct cctttttccg aataaacggc gttcttgaag ctgatcactt ctgtcacaac
     6721 ctcccttaaa tctgaatcta tattttttgg tatttgtttt tatttgcttg cctattcgac
     6781 aaattcaaat tcaaactcac cgatacgtac aaggtcgcca tctttcgcac cgcgtgcccg
     6841 aagagcttca tccacaccca taccacgtaa ttgacgagca aaacgcatca ctgtttcatc
     6901 atgatcgaaa ttcgtcattt ggaataattt ttcgatcttc tcaccagata agatccacgt
     6961 tgcatctgaa tcacgatcga tttggaactc aggaccttct ggattgaatc catagtgtac
     7021 agtttcttct tccagttctt cttcgtacaa tgggaattct ggcgttactt ctaacaaatc
     7081 tgctgtagca ttcaataacg cgtccaatcc ttgtcgagtc actccagaaa ttggaaatac
     7141 tgggatgtca tcagcaaatt catcttcttt ttccttgttc agctgttctt tgaatttcac
     7201 tagattttcc ggtgcatccg gcatatccat tttgtttgcc acgatgattt gcggacgctc
     7261 tagcaagcgt aagttataag tagataattc tttgttgatg gcaagatagt cttcataagg
     7321 atctcgtcct tccatcccac tcatatcgat cacatgtaaa attactcgtg ttctttcgat
     7381 atgacgaagg aattgtgtac ccagcccaac accttgcgaa gctccttcaa tcaagcctgg
     7441 caagtctgct gcggcaaaac ttctgccatc acttgtagta accattccta gattaggtac
     7501 gagcgtcgtg aaatgataag cgccgatttt tggacgagca gatgaaataa cggatagtaa
     7561 agtcgatttc ccgacagatg gaaaacctac aagtccaacg tctgccaata ctttcaattc
     7621 tagttcgatt tttcgctctt gtcctggttc accgttttca gcaatttccg gtgcaggatt
     7681 ccgaggagag gcaaaacgga tattccctcg tccaccgcgt ccaccttttg ccacaactag
     7741 tgtttgacca ttttcaatta aatctcctaa aagagcacca gtctccgcat ctcggacagt
     7801 ggttccttgt ggtactttaa cataggtatc ttctgaacca cgaccgtgca tccctttact
     7861 catcccattt tcacctggct gcgctttgaa atggcgattg aaccgaaaat ccatcaatgt
     7921 acgtaaccct tcatcgacga tcaagatgac atcgccgcca cgaccaccgt ctccgccagc
     7981 cggtccgccg tcaggaacgt atttttcgcg acgaaatgca accattccgt ctccgccctt
     8041 gccagctttc acatcgatcg ttacttgatc taaaaacatg gacataatta agtcctccta
     8101 ttattcattt atttatttga ctgcctgaat ttccacaata aacaaaagtc ttacttattt
     8161 tatcggtgaa atctgatttt gtctatcatt tcagcaaaaa gattgtttac cttcttcttt
     8221 ttttatcatt tggtaaggtt tccaaccttt tttctgctac caagtataat tttttgacaa
     8281 aatattcttt gcgtagcaga ataacagtgt attaattgta ttaattgagg atgccgtttc
     8341 acagcacccc taataaagga ggaacttttg atatgacagt aaaagtaggt attaacggat
     8401 ttgggcgtat tggccgtctg gctttccgcc gcatcaaaga agtctcagat gatattgaag
     8461 tagtagcaat caacgactta acaagtccaa ctatgttagc acaattattg caatttgact
     8521 ctactcatgg tacttatcct ggcacagtta ctgcaacaga agactcaatc gtagtggatg
     8581 gcgaagcaac tcgtgtttat gcagaacccg acgcaagcaa aatcccttgg gcaaaagaaa
     8641 acggtgtaga tatcgtacta gaatgtactg gtttttatac atctgaagaa aaagcaaaag
     8701 cgcatttaga tgctggtgtc aaacgagtag ttatttctgc cccagctggt gctatgaaaa
     8761 ccatcgtcta taacgtcaat gatgatacat tagatgaaaa cgacaaaatc atctcagcag
     8821 gttcatgtac aacaaactgt ttagcaccta tggcttattt cttaaataat gaatttggga
     8881 tcgaagttgg tacaatgaca acagttcatg cctacacttc cacacaaatg ctcttagatg
     8941 gacctgtaaa aggcggtaat ttgcgtgctg ctcgttcagc tgctgacaat actatcccac
     9001 attctactgg tgctgcgaaa gcaatcggct tagttatccc tgagctacaa ggaaaattac
     9061 aaggacacgc acaacgtgtt ccagtcgttg atggttcatt aactgaattg gtttcagtat
     9121 tgaaaacaaa agtaactgct gatcaagtaa acgaagcaat caaaaaacac acagttgaca
     9181 atccttcatt cgggtatgat gaccgtcaaa tcgtttctag cgatgtcatt ggaacaactc
     9241 aaggttcgat ctttgaccca acgcaaacag aagtaacaac tgctggtgat ttccaacttg
     9301 ttaaaactgt tgcatggtat gacaatgagt atggatttac ttgccaaatg attcgcttac
     9361 tagaaaaatt cgctaacttg taatttgata gatcaatcga taaaagaagc ctttgactgg
     9421 atccagtcaa aggcttcttt cttttcatcc cctactttta aactcgttca atgaacatat
     9481 tggcttaaaa attgttggac tacttgctga tatttttcag gatctgtctt gtatgcttcc
     9541 gcatgctcgg ctccttttac aacatacttt tcttttggtc ccttcgttgc acgatagacc
     9601 ttatctaaca tttcaaatgg tacaaatgta tcttcgtctc catgtataaa gagcattggg
     9661 cgctggtttt tcttcaattg ttccactgca tttgcttcac cgaaaaagta gccagcacgg
     9721 acttttgtca ctagactcgt tacttgcata agcggaaatg ctggaagact aaacatatcg
     9781 tctagctgat aagcaagctc atcatgaacg gaagaataac cgcaatcttc aatgattgct
     9841 ttcacattat ctggtagttt ttctccgcta gtcatcataa ctgttgctgc tcccatgctg
     9901 acaccgtata gaacgatttc ttgcgatttt ccattgtttt ctagcacttt gtttatccac
     9961 tgtacatagt ctttacgctc tggccatcca aatccaatgt aatccccttc gctttttcca
    10021 tgtcctcttg catcaggaac aaggacattg taacccatat catgaaacat ctttgcgtaa
    10081 ttggtcatcg tttcagcgtt tcccatatag ccatgagcca caagagctgt tttttcactt
    10141 ttcgtttcag caggaagata aatcgctgat aatttcagac cgtcttttga agtgatggtc
    10201 caatcgcttc ggttttcttt tgaactaaac catttatgtg catccgtttc tttggatgtc
    10261 tgtgttgtac caggtgtatc ttcgtctaaa aagtctttct cagaaggcac aacagcataa
    10321 ttataaaaat aattccctgc aaacactaac gcaacaatca gagctaaagc gacgcctaaa
    10381 aggattttca tccaaagttt cataaatcat ccatctcatt tctctttttt catataacct
    10441 tgaattagta taatcaaaga atctctacta tgcaatgttc atttttataa c
//
LOCUS       DACCKU010000096        10425 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.099, whole genome shotgun sequence.
ACCESSION   DACCKU010000096 DACCKU010000000
VERSION     DACCKU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.099"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..489
                     /locus_tag="IVN13_002391"
     CDS             <1..489
                     /locus_tag="IVN13_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7012846.1"
                     /translation="YQKENKIDQQSGFGIPDFFQATTNPHLPMTEFGWPIDPEGFRYT
                     LNEIYSRYRLPLLITENGIGAKDQLTEDGKIHDKYRIDYLKQHIQQMYLAMEDGVEVI
                     GYCPWSAIDLISTHEGIEKRYGFIYVDRTDDSLNTLDRYKKDSFYWYQKLIAENGLNE
                     EE"
     gene            494..1459
                     /locus_tag="IVN13_002392"
     CDS             494..1459
                     /locus_tag="IVN13_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7012847.1"
                     /translation="MQINTMTIDNEANTTLTVYLHEEKPEFHRIDKRPFILVIPGGGY
                     GFCSEREAEPIALKFAAEGYHAGVLRYHVGEHRDFHKALADAEKSMDLIALLAEKAKI
                     DQSKVAVIGFSAGGHLAAALSTLSDRKPSACILAYPAILSSFAEAMKIKAPSLDMCVT
                     PQTPPTFLFSTFEDNVVPIENSILYLRALEENDVPFETHIFQKGKHGLSLGNKWSGNS
                     EEMVDPRFAKWFELCIEWLEIIWSQAAVETTQKIEDVWQVPIKNLYAIEANRSILLEH
                     LPFLADKSRYKIIRSLSLNQLCEFSPEKFTKTKISQWVNELTWNL"
     gene            1497..3053
                     /locus_tag="IVN13_002393"
     CDS             1497..3053
                     /locus_tag="IVN13_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RuvA"
                     /protein_id="HAP7012848.1"
                     /translation="MKYEIINKNNRFYFLMDEKPCLLLGGEVHNSAASTALSMEKNIW
                     PFVNNIGLNTLLVPVYWENIESIPGIFNFDLIEAQLQKAAENKIKLVFLWFGLWKNGL
                     STYVPPWIKTDTANYFRVVNQKQEQLNCISPFCEKAIEADCRALSSLCQFISQHPLKD
                     QVCMMQIQNEVGCLETARDYSRKAQQIYGSTLPERIRELYPQANSWQELSDETFMAYA
                     FSHALEKMALEAKKWLDIPLFTNAWLHKEGKSAGDYPSGGPTKNVLNVWEKNAPSLTA
                     FAPDIYEENFREIIQDYQMIDQPLFIPETRKDLRYLSNCFYAFGKGALLYSPFGIEDL
                     RKSTEAIIDEDQAFLKELGLDQAAFDPTGSLPYFTKCYQIIKELTPFLTDETIKIHSF
                     KKEKDMTYQYQDENYRFLIKYLSKDKKEQLNTAGLFFQLENDYFFAGCNFALFHDAVD
                     EELCSEILNFEEGYFEGKKWITERVLNGDERYTPYIGNMPKIIKFSLYTYPKKEQEVV
                     HEYLADERNL"
     gene            3022..4734
                     /locus_tag="IVN13_002394"
     CDS             3022..4734
                     /locus_tag="IVN13_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HAP7012849.1"
                     /translation="MNILLTNETFKPSTSMFAQEPEPLENLSVYRNSTAAFQLLLHDG
                     KKNHYSLDDQFTIPDAIEIPIYRIEINSELPIKSNFIEYYCGKDDIFYGDKLLEEKAH
                     TYSGDRFTPIYVEIPVDGQQKTGTYPIDITIYQAGITTKETVIGQKRLEVTVEAFTFS
                     DKVTDSFHLDIWQQPSNLSRTFQVPLWSNQHFELIQTMASTLAELGQKTITVIAGEIP
                     WKGWFNYIVKDFPANLYEYSMVKVLKTKEGNIQCDFSILDKYLACFFEAGIDKEIDVF
                     GLLGVWQPPFFPLNKAVEHPEKLVIRYYDEEIEQMAFIEKQEELRQYFQQLFTYFKKK
                     NIWDKVRLMADEPKAHEIATFKEAVKCLKEIEPTLQLKVAFDKEPVLQALISEIDYPV
                     TSFYCTCQNYEWLKEQFPGKTQYYICNYPDKPNTFLHSPLTESRLQGALAYYFGTDGL
                     LRWAYNCWPEHAREDIRYNTSSLPIGDLCLVYPSVSGSLLLSLRYKELYRGVEDFYLL
                     KEAEKINKTKTDQLLADFLGGKKPENWMENSHQSVQRLFNQSYSQFDQLRKKLIAIIT
                     ETSI"
     gene            5085..6092
                     /locus_tag="IVN13_002395"
     CDS             5085..6092
                     /locus_tag="IVN13_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP7012850.1"
                     /translation="MVSIVLIATLMVLAGCRSSNQTVSGNQNQSSDTIKIAFYPNESA
                     AEFEPAREAVKAVVEKATDKKVELVTSTDYNVVVESIANGQVDLAYMGAEGYIEANKK
                     NEQVEAILTNSGPSGTLEDALYYSFLAVKPENSKEYEETGGYTINPIKDKTISFVTNT
                     STSGFTVPSNKIAEHFDLKNTDDLIMDGLFFEKVLFGGSHQGSMINLLKGDADVAAFM
                     NMPQYFDVIQGEENKAGVIYEVKKDAQTPFDTVRGEQAQVILSIPVLNGPIAANKQTL
                     SEADIDKIIKGMTADSTAENKAIFAPKDADVPGMFTKEGNERYVEVSDDFYDEIKEMN
                     K"
     gene            6117..6869
                     /gene="phnC"
                     /locus_tag="IVN13_002396"
     CDS             6117..6869
                     /gene="phnC"
                     /locus_tag="IVN13_002396"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HAP7012851.1"
                     /translation="MLLEVKNITKTYPNGNQALQDISFSLEKGEFVSIIGPSGAGKST
                     ILRTINRLIEADSGTIRFEDREMNELKRKELRKKRRRIGMIFQHYNLVSRLTVIENVL
                     HGCLGYKTTLSGAAGLYREEEKEQAFDLIEKVDLSAFAYTRCDELSGGQKQRVGIARA
                     LMQNPALILCDEPIASLDPKSAKTIMDYLKRITNELAITCLVNLHQVETAIDYSDRII
                     GVNSGKIVFEGRPYELTKEKIETIYQSKKLLV"
     gene            6881..7678
                     /locus_tag="IVN13_002397"
     CDS             6881..7678
                     /locus_tag="IVN13_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphonate ABC transporter permease"
                     /protein_id="HAP7012852.1"
                     /translation="MEKIEAEKFFRQQKRYRLFYILLIAFLYFISAHGVGFRAGAAFS
                     AVPKSFFWLFSNFIPTSDSLAYLSIIISRLVQTILMAVTATTSGAIFALIAAVIGSRE
                     IGLNRFTKVIARGMASFFRNVPIVVWAIILLLSFKQNEFTGYLAITFTTFGYLTRSFM
                     ETIDEVSGSVIEAMMASGASYLQIIWQGVLPMCLNQLLSWLVFLIENNIRDATLVGIL
                     TGTGIGFLFDFYYKKFQYDVVGMITLTVIIAVISLEAISNTVRRMIR"
     gene            7678..8565
                     /locus_tag="IVN13_002398"
     CDS             7678..8565
                     /locus_tag="IVN13_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP7012853.1"
                     /translation="MMDYQTNETNAIFNPTSYSKVAFRKRNQRIKVKLWRQERIIVRL
                     MLLLLCLVSAYTVITIDYGNVKLTEAVSAFFSNMFNIFLRPQLSGRFTTFSLVESLGI
                     TIGLSVLTTVIGAFLAFFLSLLASRNLSSGFLSNTIRGLMSFIRAVPTILWVMIFSIL
                     IGLGANAAIIGMSFHSIAYLTKVYSESIEEMESGMIESLKACGASWWQIVFQSVLPHC
                     FTTLLSWTFIRFEINFTNAIAVGAAAGAGGLGFQLTMASNFYFDFREIGVMVYMILTI
                     AVLLEFISLKLKEKYIVKT"
     gene            8592..9407
                     /locus_tag="IVN13_002399"
     CDS             8592..9407
                     /locus_tag="IVN13_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HAP7012854.1"
                     /translation="MKADLHVHSCYSNGFFHPKELLSLATAQEINHLAIVDHHTFAGI
                     EAVKEVFFESPVNLIEGIEISAFDLKRQKRVRILGYNLSNRSYIKQLCRPLLHFQTNQ
                     TLFKLKKLQAEGYHLKEEELWKEAEKATALYPCHLYRLLKKRGYGTQMDHTFYQKLMT
                     AEEVSDNRKKIDVYDAIEAIKYGGGLTVLACPGKFDNYDLIPELASFGIDGLEKYHPA
                     HRIRDLKILTDFIKTYRFSCFGGSDFHGIRGTTGFGRTLITDAREFPDQLLYN"
     gene            9502..9996
                     /locus_tag="IVN13_002400"
     CDS             9502..9996
                     /locus_tag="IVN13_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP7012855.1"
                     /translation="MINFSLSQIQDLPRIVEIYNQAIPTRLSTADLEPVTIESKKAWF
                     DAHQSKERPMWVMKIDQNIAGWVSLSDFYGRPAYAKTAEISIYIDSNYRKHGLGQKAL
                     SFAESQLLDCKIDTLLAFVFATNQASINLFRKNGFEQWGHLPKVANMDDQLTDLTILG
                     KSYE"
BASE COUNT         3486 a         1756 c         2136 g         3047 t
ORIGIN      
        1 tatcaaaaag aaaacaagat cgatcagcaa tctggctttg gtatccctga tttctttcaa
       61 gcaacaacga acccacattt gccaatgact gaattcggct ggccaatcga tccagaaggg
      121 ttccgttata cgttaaatga gatttattca agatatcgtc tgccgttatt gataaccgaa
      181 aacggaatag gagcaaaaga tcaattaaca gaagatggaa aaatccatga caagtatcgc
      241 atcgactatt tgaaacagca tatccagcaa atgtatctag caatggaaga tggtgtagag
      301 gtgatcggtt attgtccgtg gagcgcgatc gatttgatca gcacccatga aggaatcgaa
      361 aaaagatacg gttttattta tgttgatcga acagatgatt ctttgaatac tttggatcgt
      421 tacaaaaagg atagctttta ttggtatcaa aaattgattg ctgaaaatgg attaaacgaa
      481 gaggagtaag cctatgcaga tcaatacgat gactattgac aacgaagcca atacgactct
      541 aacggtttat cttcatgaag aaaaacccga atttcaccgt attgacaaac gtccgtttat
      601 actagtgatt ccaggaggag ggtatggatt ttgcagtgaa agagaagcag aaccgattgc
      661 attgaaattt gctgctgaag gttatcatgc gggtgtgctg cgctatcacg ttggagaaca
      721 ccgtgatttc cacaaagcac tggcggatgc agaaaaaagc atggatctga ttgccttact
      781 agctgaaaaa gcaaaaatcg atcaatccaa agttgcagtg atcggttttt ctgccggtgg
      841 gcatttagca gccgctttgt ctactttgtc cgatcgaaaa ccaagtgctt gtatccttgc
      901 ctatccggct atcttaagct cctttgcaga agcgatgaag atcaaggctc cttctttaga
      961 catgtgtgtg acaccgcaaa cgccacctac ttttttgttt tctacattcg aagataatgt
     1021 ggtgcctatc gagaattcta ttttgtattt gagagcgtta gaagaaaacg atgtaccatt
     1081 tgaaacacat atttttcaaa aaggaaagca cggattatca ttaggaaata aatggagtgg
     1141 aaatagtgaa gagatggtgg acccacgatt tgccaagtgg tttgagcttt gtatcgagtg
     1201 gctggaaatc atttggtcac aagccgctgt agaaactaca caaaaaatag aagacgtttg
     1261 gcaagtaccg atcaaaaatt tatacgccat tgaagcaaat cgttcgatct tacttgaaca
     1321 tttgccattt ttagccgata aaagtaggta caaaatcatt cgaagtttgt ccctcaacca
     1381 gctgtgtgaa ttttcgccag agaaatttac aaaaacaaag atcagtcaat gggtaaatga
     1441 attgacttgg aatctttgaa cttttccatc aaagaatcga tgatcggagg aaataaatga
     1501 aatatgaaat aatcaataag aacaatcggt tctattttct catggatgaa aaaccctgtc
     1561 ttctcttagg gggagaagtg cataattcag cagcttccac ggcactttct atggaaaaaa
     1621 atatttggcc atttgtgaac aatattggat tgaacaccct gcttgtacct gtttattggg
     1681 aaaacattga gtcaattccg ggaatattca actttgattt gatagaagct cagttacaaa
     1741 aagcagctga aaataagatc aagctggttt ttctgtggtt cggtttatgg aaaaacggtt
     1801 tatctactta tgttcctccg tggatcaaga cagatactgc aaattatttt agagtagtca
     1861 accaaaagca agagcagtta aattgtatct caccgttttg cgagaaagca attgaagcag
     1921 attgtcgcgc attatctagc ctatgtcaat ttatcagtca gcatccgctg aaagatcaag
     1981 tatgcatgat gcagatccaa aatgaggttg gctgtcttga aacggcaagg gattactcta
     2041 gaaaagcaca gcaaatatat ggatccacac ttccagaaag aatcagagaa ttatatcctc
     2101 aagcaaacag ctggcaggaa ctttcagacg agacattcat ggcctatgcc tttagtcatg
     2161 cactggaaaa gatggcatta gaagcaaaaa aatggttaga tatcccgttg ttcaccaatg
     2221 cttggctgca taaagaagga aaaagcgcgg gcgactatcc tagcggtggt cctacaaaaa
     2281 atgtattaaa cgtatgggaa aaaaatgcac ctagcttaac ggcttttgct ccagatatct
     2341 atgaagaaaa ttttcgggaa atcattcaag attatcaaat gattgatcaa ccccttttca
     2401 taccagaaac aagaaaagat ctaagatatc tatctaattg tttctatgca tttgggaaag
     2461 gcgcactttt gtattctcca tttggaatcg aagatcttag aaaaagtacg gaagctataa
     2521 tagatgagga tcaggctttt ttgaaagagt taggattaga ccaagcggct tttgatccga
     2581 caggaagttt gccttatttt actaagtgtt atcaaataat taaagaacta accccgtttt
     2641 taacggatga aacaatcaag atccattctt ttaaaaagga aaaggacatg acgtatcagt
     2701 atcaagatga aaattaccga tttttgatca aatatttaag caaagacaag aaagagcagc
     2761 tgaacactgc tggcctcttt tttcaattag aaaatgatta tttttttgct ggatgtaact
     2821 ttgcgctatt ccacgatgca gtagatgaag agctgtgttc agaaattttg aattttgaag
     2881 aaggatattt tgaaggaaaa aaatggatca cagagcgtgt tttgaatggg gatgagcgct
     2941 atacgccata tatcggtaat atgcctaaaa tcatcaaatt ctctttatat acctatccga
     3001 aaaaagaaca ggaggttgtc catgaatatc ttgctgacga acgaaacctt taaaccatct
     3061 actagtatgt ttgcccaaga accggagcca ctcgaaaatc tatctgttta tcgaaatagt
     3121 acagccgcat ttcagttatt gctgcatgat gggaagaaaa accattacag cttagacgat
     3181 caatttacga tcccagatgc gattgaaata cctatatacc gaatagaaat caactcagaa
     3241 ctgcccatca agtcgaattt tattgaatat tattgcggaa aagacgacat tttctacgga
     3301 gataaactgt tagaagaaaa agcacatact tattctggtg atcgctttac tcctatttac
     3361 gtagaaatac cggttgatgg tcaacaaaaa accggcacct atccaataga tatcacgatt
     3421 tatcaagcag gaataacaac aaaagaaaca gtcatcggac agaaacgatt agaagtaact
     3481 gtagaagcat ttactttcag tgataaagta actgactcct tccatttaga catttggcag
     3541 caaccttcta atctttctcg tacattccaa gtgcctcttt ggagtaatca acattttgaa
     3601 ctgattcaga caatggcttc aactttagct gaattaggcc aaaaaaccat tacagtcatt
     3661 gcaggagaaa tcccttggaa aggatggttc aattatattg tcaaagattt cccagctaac
     3721 ttgtatgaat actcaatggt caaagtcctc aaaacaaaag aaggaaatat ccagtgtgat
     3781 ttttcaattt tggataagta tttagcttgt ttttttgaag cagggatcga taaagaaatc
     3841 gatgtgttcg gccttttagg cgtctggcag ccacccttct tccctttgaa taaagcagta
     3901 gaacatccag aaaaacttgt gatccgttat tatgatgagg aaatagagca aatggcattc
     3961 atcgaaaaac aagaagagct cagacaatat ttccagcaat tgtttaccta ttttaagaaa
     4021 aagaacattt gggacaaagt tcgtctcatg gcagatgagc caaaagcaca tgaaatcgct
     4081 acgtttaaag aagctgtcaa gtgtctaaaa gaaattgaac caacattaca gctgaaagtg
     4141 gcatttgaca aggaaccagt cttacaagcc ttgatttcag agatcgacta tccagtcaca
     4201 tctttttatt gtacttgtca aaattatgaa tggctgaagg aacagtttcc tggaaaaaca
     4261 caatactaca tctgcaatta tccagataaa ccaaacacat tcttgcacag tcccttgaca
     4321 gaaagccgct tacaaggcgc attagcttat tattttggaa cagatggtct gctgagatgg
     4381 gcatataact gctggccgga acatgcaaga gaagatattc gttacaatac cagttcactt
     4441 ccaattggtg atttgtgctt ggtttatcca tctgtctccg gcagcctgct cttgtctctt
     4501 cgctataaag aactgtatcg tggtgtagaa gacttttatc tgctaaaaga agcggagaaa
     4561 ataaacaaaa caaaaacaga ccagctccta gctgattttc ttggagggaa aaagccagaa
     4621 aactggatgg aaaacagcca tcagtctgtg cagagattat tcaatcagtc ttacagtcaa
     4681 ttcgatcagt taagaaagaa attgattgcg atcatcacag aaacttctat ttaattaaat
     4741 aatagtgaat aaaaagcatg acctgcatat tgcaggtcat gctttttatt tttaaccgag
     4801 ataggaaaga atccaccgta aaaatatttc cagcacagcg agttttgtaa atctttgttt
     4861 aaaccagttt agatctagct agctgaaaag agacaaagaa ccttgttcct gcttagataa
     4921 acttttggga tagagaaatc acgaatttac atagttttta caatctttat ttgaattaag
     4981 aaagatacaa attagaactt tacaaataga gggtaaacta aagatgtagc aaataaataa
     5041 aaggagctaa gatatgtttg agttgaaaaa aatagaaaaa aacgatggtg agtatagtat
     5101 taatagcaac tctaatggta ttagcaggtt gtcgttcgtc caatcagaca gtaagtggta
     5161 atcaaaacca atcctctgat acgatcaaaa ttgcttttta ccctaatgaa tctgctgcag
     5221 agtttgaacc ggcaagagaa gcggtaaaag cggtggttga aaaagctaca gataagaaag
     5281 ttgaattagt gacaagtaca gattacaacg tggtcgtgga atctattgca aatggacaag
     5341 tagacttagc ttatatgggt gcagaaggct atatcgaagc aaacaagaaa aatgagcaag
     5401 tagaagccat attgaccaat tctgggccaa gcggaacgct ggaagatgcg ttatattaca
     5461 gctttttagc ggtgaaacca gaaaacagca aagagtatga agaaacaggt ggctatacga
     5521 ttaatccaat caaagacaaa acgatttctt tcgtcacgaa tacctcaact tctggtttta
     5581 ctgtaccgag caacaaaatc gctgaacatt ttgatttaaa gaatacagat gatcttatca
     5641 tggatggtct attctttgaa aaagtgttgt ttggcgggtc tcatcaagga tccatgatca
     5701 atttattaaa aggcgacgcg gatgtagctg cttttatgaa tatgccgcaa tattttgatg
     5761 tgatccaagg agaagaaaac aaggcaggag tcatttacga agtaaaaaaa gatgctcaaa
     5821 ctccttttga cacggtacga ggggaacaag cacaagttat tctttccata ccggtcttga
     5881 atggaccaat tgcagcaaac aaacagacgt tgtctgaagc agatattgat aagatcatca
     5941 aaggaatgac tgctgatagt acagctgaaa ataaagctat ttttgcacca aaagatgctg
     6001 atgtcccagg tatgttcaca aaagaaggaa atgagcggta tgtggaagtc tcagatgact
     6061 tttatgatga gatcaaagaa atgaacaaat aagaaaagac tggaaaggaa agccaaatgc
     6121 tattagaagt aaaaaacatc acgaaaacct atccaaacgg aaatcaagca ttgcaggata
     6181 tttcattttc acttgaaaaa ggagaatttg tttcgatcat cgggccgtct ggggcgggaa
     6241 aatcaaccat cttgcgtacg atcaaccgat taatcgaagc agatagtggt acgattcgat
     6301 ttgaggatag agaaatgaat gaactgaaaa gaaaagaact gcgaaagaaa agaagacgta
     6361 ttggtatgat ttttcagcat tataatcttg tcagtcgcct tactgtgatc gaaaatgtat
     6421 tgcatggctg tttgggttat aaaacaacac tcagtggagc tgctggtctt tatcgagaag
     6481 aagaaaaaga acaggctttt gatcttatag aaaaggtgga tctttcggca tttgcgtaca
     6541 ctcgatgtga cgaattaagt ggtggacaaa agcagcgggt cggaatcgcc agagcactca
     6601 tgcaaaatcc ggcactcatc ttatgtgatg aaccaatagc ttctcttgat ccaaaatctg
     6661 ctaaaacgat catggattac ttaaaaagaa taaccaatga attggcgatt acttgccttg
     6721 tcaacttgca tcaagtggaa acagccatcg attattccga taggatcatt ggagtgaata
     6781 gcggaaagat cgtatttgag ggaaggccat acgagttgac aaaagaaaaa atagaaacga
     6841 tctatcaatc taaaaaatta ttagtgtaaa ggatgagaga atggaaaaaa tagaggcaga
     6901 aaaatttttc agacaacaaa aaagatatcg cttgttttat attttgctga ttgcttttct
     6961 ttattttatt tcagcacatg gcgttggatt tcgggcagga gcagcttttt ctgctgtgcc
     7021 aaaaagtttt ttctggctat tctccaactt tattccgaca agtgattccc tagcctattt
     7081 atcgatcatc atatctagac tcgtccagac gatactgatg gcagtgactg ctacgacatc
     7141 aggtgctatt tttgccttga tagcagctgt gattggatca agagaaatcg gtttgaatcg
     7201 tttcacaaaa gtgatcgcta gaggcatggc gagttttttc agaaatgtcc ctattgttgt
     7261 atgggcaata atcctgttgt taagtttcaa acaaaacgag tttaccggat atttggcgat
     7321 tacttttacg acatttggtt atttgacccg ttcattcatg gaaacaatcg atgaagtatc
     7381 tggcagcgtc attgaagcga tgatggcatc tggagcttcc tatttgcaaa tcatttggca
     7441 aggtgttttg ccgatgtgtt tgaatcagct ccttagctgg cttgtttttc taatagaaaa
     7501 taatattcgt gatgctacgc tagtcgggat actgacaggg actggtattg gttttttatt
     7561 tgacttttat tacaaaaaat tccaatacga tgtggtcggg atgattacgc tgactgtcat
     7621 tattgcagtt atctcactgg aggcaatttc gaatacagta aggaggatga tccgctgatg
     7681 atggattatc agacaaatga aacgaatgcc atatttaatc caacctctta ttcaaaggtg
     7741 gcattcagga aaagaaatca gaggataaaa gttaaactat ggcgtcaaga acggatcatt
     7801 gtaaggctca tgctgctact tttatgtttg gtttctgcat acactgtgat cacaatagat
     7861 tacgggaatg tcaagttaac agaagcagtt tctgcttttt tttccaatat gttcaatatt
     7921 ttcttgcgtc cgcagttatc tggaagattt acgacgtttt ctttagtaga aagtcttggt
     7981 ataacgattg gtctctctgt tttaacaact gtgattggtg cttttcttgc tttctttctt
     8041 agcctgttag cttcccgaaa cttaagcagt ggctttcttt ccaatacgat aagagggctg
     8101 atgtctttta ttcgagcagt tcccacgatt ttatgggtca tgattttttc tattttgatc
     8161 ggattaggag cgaatgctgc aattatcggg atgtcttttc acagcattgc ttatttgaca
     8221 aaagtttatt ctgaaagtat tgaagaaatg gagtctggca tgatcgaaag tttgaaagcg
     8281 tgtggagcga gctggtggca gattgttttt caaagtgttc tccctcattg tttcacgaca
     8341 ttgctttctt ggacattcat tcggtttgaa atcaatttta caaatgcgat tgcggtagga
     8401 gctgcagctg gtgcaggagg attaggcttt cagttgacga tggctagtaa cttttatttt
     8461 gattttcgag aaatcggcgt gatggtctat atgatcttga cgattgctgt actgttagaa
     8521 tttatttctt tgaaattaaa agaaaaatat attgtcaaaa cataaaattt tatagaaaga
     8581 ggagaacggg aatgaaggca gacttacatg tgcatagctg ctattcgaat ggtttttttc
     8641 atccaaaaga acttttgtct ttagcgacag ctcaagaaat aaaccattta gcaatcgtgg
     8701 atcatcatac attcgcaggg atagaagcag taaaagaagt attctttgag tcgcctgtta
     8761 atttgatcga aggaatcgaa attagtgctt ttgacttgaa aagacaaaaa cgtgtccgca
     8821 tattgggata taatctttca aatcggagct atatcaagca gttgtgcaga ccgttacttc
     8881 attttcaaac caatcaaact ttatttaagt taaaaaaact gcaggcagaa ggttatcatc
     8941 tgaaggaaga ggaactttgg aaggaagcag agaaggcaac tgcactttat ccctgtcatt
     9001 tatatcgttt attgaaaaaa agaggctatg gtacacagat ggaccatacg ttttatcaaa
     9061 aacttatgac agcagaagaa gtttctgata atcgaaaaaa aatagatgta tatgatgcca
     9121 tagaggcaat caaatacggt ggtgggttga ctgttctagc ctgtccagga aaatttgata
     9181 actatgattt gatcccagaa ctcgcttcat tcgggattga tggtttggag aagtatcatc
     9241 cagctcatcg tataagagac cttaagatcc ttacagattt catcaaaacc tatcgttttt
     9301 cttgttttgg cggttctgat tttcatggaa taagagggac tacaggattt ggcaggacct
     9361 tgatcacaga tgcgcgagaa tttcccgatc aactactcta taattgaata aactatctgt
     9421 ttcagaaaaa tgaaaaaatg attgcagcaa ctatagataa tgatataata acctcaatta
     9481 cgtatgaggg gattgtgaac gatgatcaac ttttctttat cacagatcca ggatttgcct
     9541 agaatcgtgg aaatttacaa tcaagcgatt ccaactcgct tatctacagc tgatttagaa
     9601 ccagttacaa tagaatcgaa aaaggcatgg tttgatgccc atcaatcaaa agaacgtccg
     9661 atgtgggtca tgaagatcga tcaaaatatc gcaggatggg tgagtcttag cgatttttac
     9721 gggagaccag cttatgcaaa aactgcagag ataagtattt atattgattc aaattaccga
     9781 aaacatggat taggacaaaa agcattatca tttgctgaat ctcaactttt agattgtaag
     9841 atcgatacac ttttagcttt tgtgtttgct accaatcaag cgagcatcaa cctattccgc
     9901 aaaaacggat ttgagcagtg ggggcattta cctaaagttg caaacatgga tgatcagttg
     9961 actgatttaa ctattttagg aaaatcttac gaataataat aatgaccgct ttcaagtgtt
    10021 tttacacttg gaagcggtca ttatcataga atgtttcaat tgaaaagagt aaagtgagga
    10081 tgaatttttt taattatcat attaattaat caaataacag taaaaaaaaa tcatttttat
    10141 ctcatatgcc attcatttat catttatcct agtatcaatg agaaatagca gaaatttcta
    10201 aagaaatgtt agccctttat tttaggctga gtattatcga acgctgattt tgaactaact
    10261 gacgataata taacaataaa gtgataataa agtaattata aagtagcaaa aaatttgttt
    10321 cttgagaatt ctgcaaaaat aaggattaaa ggaagaatgt gtatgtcaaa acagtctttt
    10381 ttagttattg ggattattgc tttgctaggt attttatttt tcgaa
//
LOCUS       DACCKU010000097        10167 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.100, whole genome shotgun sequence.
ACCESSION   DACCKU010000097 DACCKU010000000
VERSION     DACCKU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.100"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            390..599
                     /locus_tag="IVN13_002401"
     CDS             390..599
                     /locus_tag="IVN13_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012856.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            806..1006
                     /locus_tag="IVN13_002402"
                     /pseudo
     CDS             806..1006
                     /locus_tag="IVN13_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1493..1672
                     /locus_tag="IVN13_002403"
     CDS             1493..1672
                     /locus_tag="IVN13_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012857.1"
                     /translation="MTELTADMSDTWLQEYLFPEKQTTARGYLPVDWEYVHKELMKKN
                     MTLKLLHREYGVHGT"
     gene            complement(1718..2323)
                     /locus_tag="IVN13_002404"
     CDS             complement(1718..2323)
                     /locus_tag="IVN13_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7012858.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2505..2840
                     /locus_tag="IVN13_002405"
                     /pseudo
     CDS             2505..2840
                     /locus_tag="IVN13_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3005..3232
                     /locus_tag="IVN13_002406"
                     /pseudo
     CDS             3005..3232
                     /locus_tag="IVN13_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3358..4329
                     /locus_tag="IVN13_002407"
     CDS             3358..4329
                     /locus_tag="IVN13_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7012859.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4469..5782
                     /locus_tag="IVN13_002408"
     CDS             4469..5782
                     /locus_tag="IVN13_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7012860.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5825..6691
                     /locus_tag="IVN13_002409"
     CDS             5825..6691
                     /locus_tag="IVN13_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7012861.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6688..7536
                     /locus_tag="IVN13_002410"
     CDS             6688..7536
                     /locus_tag="IVN13_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7012862.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7550..9022
                     /locus_tag="IVN13_002411"
     CDS             7550..9022
                     /locus_tag="IVN13_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP7012863.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQKRIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            9039..9911
                     /locus_tag="IVN13_002412"
     CDS             9039..9911
                     /locus_tag="IVN13_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP7012864.1"
                     /translation="MIYGSIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIENRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         3201 a         1728 c         2112 g         3126 t
ORIGIN      
        1 tctatatttt gatatacaaa ttacagcttc agatactaag ttgcataaac cttttccgga
       61 ccccatacaa aaggcaattg atgaattggg cataaaaaat aagaaacgat ttatattggt
      121 gattcattat atgacatgca aagtgcgaaa gcttgtggga tagcatttgg tttagctaaa
      181 tggggagcca aaaatataga aaattttcaa agtgtagata taattattga ttctcctcaa
      241 gaaatacttg atttgttaga aaaatagaca aagagcgtga gataaggata gggtctcacg
      301 ctctttgttt ttcgccattt aaagattgaa atttttgcca tttaaaaaga aaagtgacag
      361 ataggaataa aaacttatta aatgtagtta tgatctgtta tatttcattt ctaatactag
      421 gtcttccaca agcagtgttt cctgaaatgt tttctggaga ctggggtatt gtcagctatt
      481 ctatattaac attgattgct atggttttag gtacttcatt ttataatgct tttgtaagta
      541 agcatgttga ggaatatgaa gtattaatgt acttaaaaga gaaaaaaaca agtaattaat
      601 atgagaacac cacgcatagt ttttattttg agatattcta gaatgaaaca cattaagaac
      661 gaaagcaaag caaaagacgc cagaacttct actaccttct aagttttttc atatttgttt
      721 gttatgctat acatagatct gcataattag acttgcgctg cccgttattg aagtttcagg
      781 cagcgctctt tttgggggat cgataatgga agaagaaaaa atatttgaga aacgctggca
      841 gctagcatca agtgaacaaa gagcaagata caataatttg atcacctcct accccactgt
      901 tgactagaca tataaggaaa agaaatactt actttggctg tgccagttag atatagatac
      961 ctttgaaaca tttgaagtga tcctagataa aattaaacag aattgatcag aaacaagcga
     1021 agccttctct cggttcgctt gttttgctat ttattagttg gctcagggca actctaagct
     1081 cgtttgaaag aaaaccgatg gataatcccc ttgtttgtac cactttagaa tacatggatg
     1141 tacgtattgc cattgactcc tattagttac tgaaaaaaaa tttaaacaag ttgagtattg
     1201 cagataaata gctccagtga ggtgaataac tgctccactc aaggcaatag atagctccat
     1261 ccagggcaac aaactgctcc actttcatat ttaatagatg aataaccttc tgtataatag
     1321 aaaggcatac ctttcggtat gacaacttat acagaatgtt attttattgg ttcactatcg
     1381 aaaaattttg gagctttatt tttccggtat ttctcaacga acgattagtt taactgtggg
     1441 aagctccaga catacgattc gagaagttac ttctcgagca gacaggaagg gggtgactga
     1501 actaaccgct gatatgagcg atacttggct gcaggaatat ctttttccgg aaaaacaaac
     1561 aacagcacga ggttaccttc ctgtagactg ggaatatgtt cataaagaac taatgaaaaa
     1621 gaatatgaca ttgaaactcc ttcatagaga atatggggta catggaacat agaaacgaaa
     1681 atgcacggta gaaaagcgaa tgaatatagt ataaattcta caaatcattc gctttcttta
     1741 ttttatatat ggttggtcga gagagaccat tttctttggc aatttctgca actggaactc
     1801 cttgatttaa catgtccacc acagttttaa agactaatcg tttttgcgga tctgttgagt
     1861 tctcagaata ttcaattggt cgccccttat atcgaccagc ttttttggcc aataatactc
     1921 cttgccgctg acgttcacgg attttctcac gttcgttttg tgcaatataa cttagcaatg
     1981 ataaaaacat atcgaacata gcagtatcta ggagttcatt tccagtatta aaatctaaaa
     2041 actgtgcatc tagtattttt aatgctactt ttttctgttt cataaaggta actgtatttt
     2101 ttatgtcttc ataatctctg cctaggcgat ccagtgatgc aacaatgaca caatcacctt
     2161 ctcggatata atttagtaat ttttgaaact caggtcgctg gatatcttta ccactggctt
     2221 tttcttggaa taatttatca atgttaaggt cttccatcat tgccaattgt cgatctaaat
     2281 tttgatcttg tgaagacact ctaatatatc caatttttgc cataaaaact ccttttaaaa
     2341 aatgatataa tttacatgta aaatttactt gtagttttac tgttatcata gcatttttaa
     2401 aaataaattt taagtctact tataatttac tttggaggtt ttgaatgaat gaaattaagc
     2461 gcaataaaaa ttcttttggt gtttatttag gagagcctag ctcggaacag ttaaataaag
     2521 gtgaagctcg tcatgctcta tgtcgaaata tattttatgg tagaaaaggc aaactttatc
     2581 aaacttatta cgacggtatg gaggaacagc tcaacgcatt aggattagta actaatgcta
     2641 tcatatattg gaattcacta tatttagaaa aagtctttga acaaattaaa acggaagggt
     2701 tcgactgttc cgaagaagtg ttacgcaagc aatttccatt aattagtgat cacattaatt
     2761 ttgttggcaa atatagtttc cgctatgaaa aagaattatc gaatggaata ctacgtccgt
     2821 taaatataga gaattattga gaatgttcgt cttttggttc ctaccgtgca ttttcgcacg
     2881 gatgttccag atactatttt tgaatgtata aaattgaaaa cacaagaata ctaataaatc
     2941 aacgattctt gtgtttttaa tttttcacta ccgtgcattt tcgtttctat gttccatgta
     3001 ccccaatctt gaaaaattaa ttaaagactt agataaagcc attgacgact tagtcgtggt
     3061 gctaccggag tatcaatgtt taaccagtat cccaggtatt ggtcctgttt aggctgctgg
     3121 aatactcgct gaaattggtc caattgaacg tttttctgat gaaataaaat tgacaaagta
     3181 tgccggccta tattggcgac agagtcaatc cggtaaatct gaatacgaaa atttttttac
     3241 gaaaagcttc acgtctaata tctattttaa aataaaaata ttcctgctaa tttggaatcg
     3301 tttccttctt ttggtataat aatacctgaa attcttttga gaggagttgt tcgtgaagtg
     3361 aaaactacta tgaaggatgt tgctgcgtta gcaggagttg gtgtgggtac ggtatctaga
     3421 gtaataaatg gagtaaaggt gaaagaggtt accaaagaaa aagtaatggc tgcaattagt
     3481 gagcttaatt atgagcctga tgaatatgcc agaggtatga aaacgaatag tagcaataca
     3541 atagctctta ttttacctac gatttggcat cccttctttt ccgagtttgc ctactatgta
     3601 gaggaagctc tcagccaaaa aaattataag ctttttttgt gtaatgcgaa cggcagtgcg
     3661 gaaaaagaaa aagaatatat ccaaatggtc aagcagagta aagtagacgg aattataggt
     3721 attacctatt cagacattga taaatatgta tcatcaaatt taccatttgt cagtatagac
     3781 cgccattttt cggaacaggt ttcttatgta acggcagata attatttagg tgggcaaatt
     3841 gccgctcaag aactacttac tcgtaactgt aaaaacttgt gctatgttgg gggtgtttca
     3901 ccttacccta acgaaacaaa caatcgtaaa gctggttttt acaatatttg tgaggaaaat
     3961 aaaatagctg tgaaggtatt ggatatgcct gaaccaatta gcaatttaga ggaacaattg
     4021 ctcgcttttt tttcggataa tcctgaaatt gatagcattt ttaccatcaa tgatttcatg
     4081 gctttggatg tgataaaagc ccaaaaaaaa ctagggaagg aagcacctaa ggattttcaa
     4141 attattggtt ttgatggagt caagctatcg ttgcaaaaag actatttact atcaacgatt
     4201 gtacagccga tagaggaaat ggccaaggtt tcagtagaaa cactcatggc gatgatttta
     4261 aatgaagaac cgcctaagcg tgtggtagta ccggttttct ttcacgctgg agagacgact
     4321 aaagagtaaa aaaccggttt ataacgattt ttttgagata ggttgacaga aagtgatttc
     4381 aatgatatct taaaaccaat taattggaaa cgttaccaat taattgaaaa ataaaacgga
     4441 aacgtttcaa aaaataggag gaacatcgat gaaaaagaga atgttagcaa ttgcagcaat
     4501 tttagcatta acagctggag gacttgcagg atgtggcagt aaggagaaaa gttcaggtag
     4561 cagtgatcca ggagaaatta agatttgggt tcagttttct gatgaaacgc ctgaaggaaa
     4621 agcttgggca gagattgtga acaattttaa tgatgaatac gagggtgaat ataaagctgt
     4681 tactgagtat attcctcgta gcggaagcgg cggtggatat gaggataaag taaatgctgc
     4741 aattacaacc aatacccttc ctgatgttat tacgttagat ggaccgaata ctgcagctta
     4801 tgctcattca ggagtcattg ctccattaga tgattatttg aaagatgcgg acatggatga
     4861 tgtattagac agcattattc agcaaggaac ctacgatggc aaaatgtatg cgtttggttt
     4921 ctcagagtcc agtgtgggtg tctattacaa taagtcgctg ttcaaagatg ctggaattac
     4981 agaagatcaa ttgccgacgt tagaagatcc ttggacttgg acggaatttc aagcaatttg
     5041 ccaaaagctg actgataaat acaaaacgcc tgcaattgat atgcaaatgg cgtcgggaga
     5101 cgaaatgctg acctacgcat atacaccatt tatatggtcg aatggcggcg atgtcgttaa
     5161 tgctgatgga accaaagccg aagactattt taacggtaaa aaatccgttg aagcgttgga
     5221 atttattcaa gaattggttg aaaaaggtta caccacaatc agtcctattg aaaaaggatt
     5281 tgaaactggc aaatatgcca tgatgctgag cggttcttgg acaatggcag atttagagga
     5341 aaactatcaa gagattgatt atggtatttt gccttacccc gtttcagatg caactgggga
     5401 gttagtttca ccaaccggaa gctggcagtt agcaatgact gagaaaacag agaaaaagga
     5461 tgcttctgcg gcctttatta agtttgcaac cagtacagaa tctagtcgta ttatgagttt
     5521 agggaacagt gttctaccta tcagaaattc aacagttgat ttaattaaag atgaagtgtc
     5581 ggaaggaatg caggtattaa ttaagcaaaa ccaactttct ggacatgccc gcccagtggt
     5641 tgtagcttat ccgcaagtat ctagagcatt ccaacaagca gttcaagata ttagctactt
     5701 cgaggaaaac ccagatgtac aaaaagtagc agatagtcgc gctgctgaaa tgcaacaggc
     5761 aattgatact tcactaaaat agaagtttct aaagggggcc aatcaagtgt taaaaaaacc
     5821 gacaatgaaa gacaatttga taggttacgg ctttctagcg ccagctttgc tgctgctgac
     5881 gatttttctg atcatacccg taatcatggt aatttattat gcctttactg attattattt
     5941 gttaacgcca gatgctagac aatttatcgg cttagaaaat tttaagcgac tgttcgctga
     6001 tccaattttt gtaaaaagta tttggaacac ggctaaattt gttgtttgga ttattccggt
     6061 ccaaataggg gcagctttag gaatggcact tattatcaac aaaaatcgta aaggaaatat
     6121 cttttttaaa gtagcctttt ttgcgccagt cgtgatgtcc ttagtcgtta tttcgatttt
     6181 atggttgtac ttgttaaatc ctaatgaagg tctggttaat acgctatttg caaagattgg
     6241 aattgcttcg cagccttttt tgactagtcc caaacaagca atgtatacgc ttgttttcgt
     6301 atcggcatgg caaggtgcag gatatcagat gctgctgttt ttagggggaa tgcaaaatat
     6361 tccgcaagac gtttatgagg cagccgaaat tgatggtttt tctaagtttc agcaatttcg
     6421 ctatatcacc atgccattat taaagcccac ggcgattttt attttactta ccacattgat
     6481 ttcggccttt aaactaatca ttcagccaat ggtaatgact caaggtggac caatgaattc
     6541 tactatgaca atggtttatt acatttacca aactggattt acagaccgta tggtaggcta
     6601 ctccagctcc attgcgttaa tctttaccac tatgattggt ttaattacaa ttgctcaaag
     6661 aaaattggta aaggaggatg atccagcatg accaagaaat ttggaccgct aatgatcgta
     6721 gaatattgtt tgctgttcct tttagcggca ttattcattt tcccgatgct gtggatggta
     6781 gtttcctcta tgaagcctga agcagatgtc tatcaaaatt tagcttcctt caaagctttt
     6841 ttacctagtt tgaatccagc taattggttt aaaacttatc aagaggttat ttcccggttc
     6901 agtgtgggaa cttatttatt aaacagtatt ttttatggtt taacctttgc ctgtgggtca
     6961 atcattgtaa actctttggc gggttttgcc tttgcgaagg ttaatttttc aggcaagaaa
     7021 attatctttg gagctttgtt agctctgctg attgtcccga tggagacggt tttaattccg
     7081 caatttacca tcgttaattc actaggctta gtgaatactc gcttggcagt gattttacca
     7141 gcgatggcca gcgcgtttaa tatctatctg tttagaaatt tctttatcgc gattccagaa
     7201 gaaatcattg aatcagcgaa attagatggt gcaaatattg ttaaaatttt ctttcacatc
     7261 atgctgccaa tgtctaaacc agccatagcc acagtaggtg tcttatcctt tatctccagc
     7321 tggaatgatt atatttggcc gttaatggtc ttaactgaca gcagcaaatt ttcaatgcaa
     7381 gtggcaatta ctacgatcaa tactacacag ccagtttata tcaatcaagt gatggctgtt
     7441 ttaaccattg caacgattcc actgattatt gtttatatcg ttgcacaaaa atatattctg
     7501 cagggacttg gcggttctgg aacgggtata aaataggagt gaatagtgaa tgttgcaaaa
     7561 acttttaaag gccaatgagt ttattgagaa aaatgctcaa acagtcaatc aagaataccg
     7621 gaataaattt catttgatgg ctccgattgg ctggatgaat gatccaaatg gttttgttta
     7681 ttatcaagag gagtatcact tgttttatca atattatcct tacgacagca tctggggacc
     7741 aatgcattgg ggccatgcta aaagcaagga tctaattcac tgggagcatt tgccagtagc
     7801 actggcacca ggtgaaagct atgacaagga aggctgtttt tccggcagtg ccatagaaaa
     7861 ggatggcaag ctgtatttga tttatacagg acacaatgtt gtggaagggc aagttcgaca
     7921 agttcagtgt ttggcagttt cagaagatgg cattcacttt gaaaaatatg ccggaaatcc
     7981 aatcatcgca gaagatcact tagcttctgt tgcaacgacc gaagatttcc gcgatcccaa
     8041 agtgtttcag cgaaaagaag actattttac tgtagtcgct actaaaactg cagatgctcg
     8101 tggacaaatt gtgatgttcc attccaaaga tttactaact tgggagtttt attccgtatt
     8161 gctggaggga actccagaac aaggcattgt atgggaatgc ccagacttgt ttgaactaga
     8221 tggcaaagaa gtcttgattc tttctcctat tcaaatgact ccacaaggca atgcttacca
     8281 aaatacgaac tcaactgttg ctttcatcgg tactgtcgat tggcaaacag gcaaatttct
     8341 agtagagaat tttcatgaaa tcgatggcgg tttagatttt tatgcacccc agacctgtga
     8401 aaacgaagca ggccaaagaa taatgattgc ttggatgcaa atgtggcagc gtaaaatacc
     8461 tacgaatgat ctgggccatg gttggtcagg ttccatgacc ttgcctagag agctgagggt
     8521 ggctgataat caattgttgc aacggccagt ttcatcaatt tatcaagtaa ttgatccgca
     8581 gaaaaggatt gaatgggcga agactgaaga gaatcgcttc ttactgaagg acttagtata
     8641 tgaccagcac tatttacgct tggaaatcaa tttaaaacag gcaacgaaac tagaacttca
     8701 ctatgcgaag ggacccgacc atacattact gcttaactat gatgttgaac gggagtgctt
     8761 tactgcttcc agggaacaca tgccgtatga tttatcagga gctgaaaagg aagtgttgaa
     8821 aaagcgcagt gtgacgattc ctttggaaaa taattgtcta accatcgaaa ttttccgtga
     8881 tacttcagct attgagcttt ttctaaatga tcgtctgact atgagtacaa ctttttatga
     8941 gacagaaaaa ggacaggatt ttcttttgct ggcagacagt gaagtaacct tttcattgga
     9001 aagcggctta attaatctat agaagggaga gctcagtgat gatttatggt tcaatcgaag
     9061 caggcggcac aaaatttgtg tgtgccattg gcgatgaaga aatgacgatc aaggaaagag
     9121 tgagctttcc gacaacgaca cctgaggaaa caataccttt agtcattgac ttcttcaaac
     9181 aataccaagc agatttagca gggattggga ttggctcatt tgggcctatc gatatccatc
     9241 gcgattccgc cacttatggg tacattacct cgaccccgaa agtggcatgg caaaattttg
     9301 atttcatcgg taccatgaaa aaagaatttc cgatcccgat ttcttggaca acagatgtca
     9361 atgcggctgc ctatggtgag tatgtcttcg gcagtggcaa aggattgagc agtgtcgttt
     9421 actatacgat cggtacgggt gttggcggcg gtgccttaca agaagggcgc tttatcgaag
     9481 ggtttagtca tccagaaatg gggcatatgc tcgtcgtccc tcatccaaaa gatgactttg
     9541 ctggcagctg tccatttcat ggcaactgtt tagaagggat ggccgctgga cccgccatcg
     9601 aaaaccgctt agggaaaaaa ggacaagagg ttgcagaaga tgatccttat tgggaaatcg
     9661 aagcctccta tattgctcaa tgtgcctaca atacaacctt gatgttttct ccagatgtga
     9721 tcatttttgg cggtggtgtg atgaaacaac gccatatgct gcaaaacgtc cacgacgcat
     9781 ttgcaaaatt ggtcaatggg tatgtcgaaa cacctgagtt aagcaaatat atcgtgaccc
     9841 cagcattaga agacaacgct ggaacgctcg gttgtttggc attggctcga gaagcggtct
     9901 tacattcata aacgaaaaaa ggctcttcga catttagtgt tggtagagaa agatacaact
     9961 gaaaatttag tcataaccag ttaggcagag tgcttaactg gtttttgttt tacttggaag
    10021 atcaagcctt gaatgagata aattcttgct cgaaagttat ggaaatttcg atagccgtag
    10081 gccacttgta ttgtgcaata gttttttaca aaaaaaaggg ggtgcggata tttttttgga
    10141 cctttttaac aaatatatct ttgcttc
//
LOCUS       DACCKU010000098        10121 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.101, whole genome shotgun sequence.
ACCESSION   DACCKU010000098 DACCKU010000000
VERSION     DACCKU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.101"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(277..2169)
                     /locus_tag="IVN13_002413"
     CDS             complement(277..2169)
                     /locus_tag="IVN13_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012865.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2166..3893)
                     /locus_tag="IVN13_002414"
     CDS             complement(2166..3893)
                     /locus_tag="IVN13_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012866.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3909..4559)
                     /locus_tag="IVN13_002415"
     CDS             complement(3909..4559)
                     /locus_tag="IVN13_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP7012867.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4763..5059
                     /locus_tag="IVN13_002416"
     CDS             4763..5059
                     /locus_tag="IVN13_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP7012868.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5251..5871
                     /locus_tag="IVN13_002417"
     CDS             5251..5871
                     /locus_tag="IVN13_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP7012869.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5963..7129)
                     /locus_tag="IVN13_002418"
     CDS             complement(5963..7129)
                     /locus_tag="IVN13_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP7012870.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7141..7494)
                     /locus_tag="IVN13_002419"
     CDS             complement(7141..7494)
                     /locus_tag="IVN13_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP7012871.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7622..8644
                     /locus_tag="IVN13_002420"
     CDS             7622..8644
                     /locus_tag="IVN13_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP7012872.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9083..9517)
                     /locus_tag="IVN13_002421"
     CDS             complement(9083..9517)
                     /locus_tag="IVN13_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012873.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9627..>10121)
                     /locus_tag="IVN13_002422"
     CDS             complement(9627..>10121)
                     /locus_tag="IVN13_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP7012874.1"
                     /translation="LENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSD
                     TIQAKNDKQTLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQL
                     LLSLEQELPAFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGF
                     FEHE"
BASE COUNT         3021 a         2050 c         1835 g         3215 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca ggtttgttga ctattgtgac ttgtgaccga
       61 ggagttggca ggagaacacc gtttaaggaa gaggttgtaa aatcagcggt ttgacctgag
      121 tattagagat acaaaacgga aaaatagctt ctcatatttt gccgtttttt gagctaatac
      181 cgaaggacag ctgattgaac accgtttatt cggaaaaagg gactgtgaca cgacttttgt
      241 cacagcccct aaaaaaaata ataaatttgt actattctat gaagcttctt cttcagaaaa
      301 ctgactgtta taaagatctg catagaatcc gtctttttcc atcaatgtat cgtggtttcc
      361 tgtttctacg atcgaaccat gattcatcac aataatgttg tctgcatcgc gtattgtaga
      421 caagcggtga gcaacaacga aacttgtccg gttttccaat aggcggttca tcgctttttg
      481 aatcagtatt tctgttcgtg tatctacact agatgttgct tcatcaagga tcaggacatc
      541 tggatttgct aagaatgccc gcgcaattgt gattagctga cgctgccctt gcgaaatatt
      601 gcttgcatcc tcgtttaata cagtgttata tccttctggc aatttacgaa caaaatcgtc
      661 tacgtgagca gctttagcag cctcaatgat ttgttcgtct gttgcatcgt cgttcccata
      721 tttgatattg tcatagatac ttcctgtaaa cagccaagta tcttgcagta ccattgaaaa
      781 ctgtgcgcgc aattcatcac gtgaaagatc acgggtatct acaccatcgt aacgaatact
      841 acctccagaa acatcgtaga accgttcaag aaggttgatc agtgtcgtct tccctgcacc
      901 agtcggacct acaatagcga ccatctcacc aggtttgaca tccaagttaa aatcagtcat
      961 tagcaagtta tctggtgtgt agccgaattg aacattttca aaacgaactt tgtatggtgt
     1021 atctttttct tctggaaggc cagatttttc attggacatc tcttcttcat ccaacacttc
     1081 aaatacccgt tcggctgaag caactgttga ttggatagtg ttcatcaaac ttgcaagttg
     1141 agtgattggt tgagagaact gtgttgtata ttgcaagaac gcttgaacat cccctaatgt
     1201 catgttccca tttgcaactt ttactccgcc tagtacagcg acgaacacat agcctagatt
     1261 tttaacgaaa ttcatcaacg gcatgatgat tgccgaaata aattgagctt tccaaccagc
     1321 ggcatacaga ttctgatttt ctttttcaaa tacttcctgg tctttttcag tatgattgaa
     1381 tgttttaaca atggtatgac caccatatgt ttcttctact tggttattca ataaacctaa
     1441 acttttttgc tgatctgcga aaaacacttg tgatttcggc gctacgatca tcacgatgat
     1501 caagcttaaa gggatcatca ataacgcgac taacgtcaat tgccagctga ttgaaagcat
     1561 catcacgatg accccaatca atgtgactgc actagttaca aattgagtca aattttgctg
     1621 cagcgtgctt gcgatgttat ccatatcatt gattgctcga gacatgatat ccccatttgt
     1681 atgtgtatca taataggaaa tcggtacacg attcattttg gcttccaaat catgacgtaa
     1741 ttcataaact gttcgttggg aaacacgtgt cataataaat tgctgtaaaa agttgaatac
     1801 agcagaaatc aaatacatca cgattactgt cgcaatgatc tgcgcgattt tatcaaagtc
     1861 tatcggaaat tttgttactt gttggccttg ttgcatctgt tgagcgccag ccattacacc
     1921 tttgaatatt tctgtggttg ctttccccaa gacctttggt gtttggattt ggaagatcac
     1981 ggctgctatt gctagtacaa atacagcgat gatcgcaacc atacgtttag acatatagcc
     2041 aaacaatcgg cgtaccgttc cccagaaatt tttaggcttt tcacctttta caccgagatt
     2101 tctgccaggt ccaccatgtc ctggaccgcc tgctggtcca tgagagggac gtgtgtttgt
     2161 tttactcatg ctaagtcctc ctctcttagt tgtgaatgaa cgatttcttg ataagtttcg
     2221 tttgttgcta atagttcttc atgtgttcct ttgccaacca gttttccttc atctagcaca
     2281 aggatcatat ctgcatccat tactgtactt actcgttgag caactaaaac ggtgatactt
     2341 tctttcatat tcgttttcaa tgcttggcga agattggcat ccgttttgaa atccaacgct
     2401 gagaaggaat catcaaacac atagatatct gctgttttga ctaatgcccg tgcaattgcc
     2461 agacgctgtc tttgtccacc agagaagttt cctcctcctt gttccacatg gctatctaag
     2521 ccatcttcca tctctgaaac aaaatcttta gcttgtgcga tttctaatgc tttccaaatc
     2581 atttcatctg ttgcatccgg cttaccatac tgcatattgt ctcggatcgt tccagagaac
     2641 agaactgctt tttgaggaac aaagcccatt aattctcgca aattatattg agtcatgtca
     2701 ctaatgtccg ttccatttat ttggatcgaa ccagattcga tgtcatataa tcgagggatc
     2761 aaattgacta aggtcgtttt acctgaacca gtccccccta tgattgcgac gatctcgccc
     2821 gacttggctt ggaaatcaat atcttccaat gcaagttttt ctgctccatg atatcgataa
     2881 ttgacatgat tgaaggccaa tgttgcgttt tttccagcaa aagaaactgt tttaggattt
     2941 tcagggtcat ggataccgat tttttcatcc aacacttcat taatacggtc agctgaagct
     3001 tgtgcacgag gcaccatgat aaaaatagcg gaaagcatca taaagctgat caagatctgc
     3061 atcgcataag tcataaatgc aatcagatta ccgacttcca aagtcatatc tgctatgtag
     3121 tgtccaccaa accaagtgat tgctacgttt gtaccgctca tgatcaatgt catcattggc
     3181 atcatcaatg cgacgatcgt attaactttg attgccgtat ttgtataatc tttattcgct
     3241 aaatcaaaac gattttcttc aaagcgtgtc ttatcgaatg ctcgaatcac tctgacccct
     3301 gtcaatcctt ctcgaaatac caggtttaaa cggtcagttt tcttttgcat acttttgaat
     3361 aatggaacag caaagtacat gattccgccg atcaaaataa tcattactgg gatgacaact
     3421 aagaagattt ttgtcaactg atgatctttt tgataagcaa gtatgctcgc gccaatcaac
     3481 gtgattggtg cattgatcat catacgtaaa aacatttgtg taaccatttg gatttgagtc
     3541 acatcattgg tcgttcttgt gatcaaagag gccgttccat atttatcaaa cgcatctttt
     3601 gtcagactct ctgatttttt atagacatcc gtacgcaatt gcttacccag tttctgtgat
     3661 tcacgagtag caaaaaatgt atttgcgata gcagctaaga tactgatcaa agaaaagccg
     3721 atcatcacaa aacctgtatg ccaaatataa tcgacatctc cttgcgctac ccctttatca
     3781 ataatattgg aggtaagtgt cggcaaatat aaatctgcca gtacttggat caccatgaaa
     3841 actgcagctg caattgctga agtcaaggag atccgtttta cgatttttag catctagttc
     3901 ccttctttct atttatatac tccgtttttc agccaagaaa gtttcaaatt gaattcactg
     3961 attgcttgct ctactgagta atcttcagat gttgaaaata agcgatacga atgcgcaatt
     4021 gttgtaaata cagtattcat cactaattgg atcagtaatt ccaattcatg atcatttttt
     4081 actttcagct tcgtccgatc gatcaattca agcaactctt ttgcatcttg tttgtgttct
     4141 ttatgtggat cgtgaggcgt tttttggcga ctcgtatgag gagcgacttt tcttgaggca
     4201 cggtaatcca tgttcataaa caagttacga taaaaagaag cattctcgcc ttccaaaatc
     4261 tcataaatca ttttagaaaa ataggattcg aatcctttga atagatcacc atcggattct
     4321 ttcattagtt gtaacatatc tttagagctg tcacgtctca ttgtctcaaa gtaataaaaa
     4381 taaagatcct ctttatcttc aaaatactga tagaaactgc ctctagggat atccgctaat
     4441 ttgacgatat tagcaatcga agcttctttt aagggtactc tagaaaactc gattctcgca
     4501 gcctcgatta gtcttttttg tttttcttta ggcaaatgga aaaatgtctg tgtcggcata
     4561 tggattctcc tttctttcaa gatgacacat tgtcatatga cgctatgtca ttataacacc
     4621 tcctacctgt tttgcaagac atttgcatag tttttttcat tatcatccga tacattaaaa
     4681 aagtgataag aggaatcctt ccatttgaat taatgagagt tgcccattaa aatagccaca
     4741 tactaaacaa aggatgagat aaatggccca atcaattagt gtccaagact tcaataaatt
     4801 gcctctcgat gctgacagca tcgttttaga tacaagaaat accttagatt acacaaaaaa
     4861 ccacttaagc cactcgcttt ctatccccgc tgctatcttg ccaagacaat taaaaaatct
     4921 agatccaaag aaactttatt atattctaag ctatacaggc aggcgatcag aagcattagc
     4981 cgatcaactt actgctaatg gttttcgtgc tgtttatatc attggtggga tggaagctct
     5041 tcagtcgagt gttgcttgaa tattgcttgt tagattcaaa agaggctgtg acaaaaaatc
     5101 gtgtcacagc ctcttttata ccctgtcttt ttttagttat ttattcttca accttatcaa
     5161 tatgacttga tagccttttc tttttcgagt aaactgattt tgttagtcat agttataaca
     5221 atcatagcaa taaaaggaga aggtaagcat atggcatctg ttttagtagt caaaggtcat
     5281 cccctaactg cagaagaatc gcgcacagtt aaggcattga cgtctttttt aacaagttat
     5341 aaagaaaatc atccagatga tgaagtgact gttttggaac tttatcgcga tgatattccg
     5401 gaaatcgatg aagaattatt atcaggttgg gaagcattac gtgctggtgc agaattcact
     5461 tccttatcag aaagtcaaca acaaaaaatt gctcgtttca atgaattgac tgatcagttc
     5521 cttgctgctg ataaaatcgt tatcgcaaac gccttatgga atctaaatat cccaacaaaa
     5581 ttaaaagctt ggttcgatac cgtcaatgta gctggaaaaa cgttccgcta tacagaaaac
     5641 gggccagaag gattagtgac aggtaaaaaa gcactgcaca tccaatctaa tggcggtgta
     5701 tacaacggac aagactttgc ctctcaatat gtaaaaggta ttttaaactt tgtaggcatc
     5761 gatcaagtag atcagctatt catcgaagga atcgattatg accctgaccg tgcagatgag
     5821 ttgatgcaaa atgcattaga taaagctgct gctttaggta aatcatttta aaaattgaga
     5881 taaatgcata caaaagagac agaggaaaaa atcttcaaag cagcatttgt gccttggata
     5941 ttttcccatc tgtctctttt tgttattctt tctgcttttt caacggattt tcttcataaa
     6001 gagaagagat tgttgtgtac cattcaaaaa catgagtaat cactacttta gctgctgcat
     6061 aaacaggaat ccccaagatc acaccgatga ttccgaacaa tttcccagaa gtcagcagaa
     6121 ctaataaaat cgttactgga tggattgcta actgattccc tagtactaat ggagagatca
     6181 agcgtccttc tattgtctgc tctactgcaa aaacgatcag tactttgatg atcatgaatg
     6241 gacctgctac aatggctaaa aagatagccg gaatcatcgc taaaaatgat cctaagtaag
     6301 ggatcaggtt taaaaaaccc gcaataatcc ccaacgtgac agcatactct aaaccaatga
     6361 ccgcaaaacc aatcataaac atgattgcca cagcaaaagc cacagttagt tgtcctctta
     6421 tataagaaga aacttgacta ttcatttcac tcagcacatt caacgtcggt ttttgcatgc
     6481 gtgtcggtaa aaatttgaca aaataaggtg ctaattgttt accgtctttc aatagataaa
     6541 ataagataaa cggcatcgtc agcagagcca ctaaaatagt cgctaccgcg ccgacaaaac
     6601 ttccaagact ctgaactgta ctcttagata tatcttggat catatcgcct gccgaattca
     6661 tgaacttatc acctgctgtt tccaactgtt cgcgaaattg gttgaacagc gggtcccgca
     6721 aaatctcggt taatttatta tcaatcgtat ctacatattg agggaagtta ctgatgaagc
     6781 tcactgtttg ttcctgaatc ttagggatga tcactacgct cccccatgcg attagtgcaa
     6841 caaccaaaac aaacagccca atgatactgt aaagccttgg tacttttcgc ttttcaaagt
     6901 aatcaactac cggattcatc aagtaataca agattcctgc taaaatgata ggcagaccta
     6961 cgattgctaa aaattgccag attggtgcaa acaaatatga aacttttgta aataaaaaga
     7021 cgatcagcaa tattaacaat acaacaagta aggctgtgac tacttgatta tttaaaaacc
     7081 agcgccaaaa ccacgatacc ggtctgttct tcttattttt tccttccata attcctcctg
     7141 ttagttataa aaaatttggc ttcccaaacc aatagcaggc agtctatgcg gatgaagata
     7201 gattccattt gcaatttgtc cttcttctgg acaagcatca aaaatcaatg atacgtgagc
     7261 tactgtcttt aaattcccat tggccatcgg accgacataa tcgatcgtat atacctgatc
     7321 atcaaaagca attgttccgc ctgctttcaa agaaaaaggt tcgtcttttg atacttcttg
     7381 aatcaccgaa tattttcgta aggcagctgt tgctgtttta tcgaacaaaa tcagcatggg
     7441 ttcttccttg tttattgctt ctgtcccaat ttctgtaatc tttgcctgaa tcattgcact
     7501 ctctccttta attgctttca aaaacagtat aacacattct cattctttag ccgtgtggaa
     7561 taattatgtg atagaataaa aacgaaaact atttcattac aattaggagg aatcagctca
     7621 tatgatcgaa aagataattt taggaaccta tacgcgtcgc gaaagtaaag gaatctatac
     7681 gatcgacctt gatacagaaa aagaagaatt agccaacctt tcattagctg ttgaagaaaa
     7741 tagccctacc tatgtagcaa aaagtaaagc tggcaatctt tacacagtta ctagtgtgga
     7801 tggattaggt ggtgccggtg cgtacgataa ggattttcat tttttgaatg cggtaacaga
     7861 gtctggcgct cctttgtgtt atgtagcggt tgatgaagca cgtcaattac tatacggggc
     7921 gaactatcat aaaggtgaag taaatgtcta ccatatatta ggagatggcg aactgaaagc
     7981 agcagattca atattccatc aagaagcgac aggtcctcat aaaaaccaag atcatgcgca
     8041 tgtccactat acggatctga ctcctgacca acgattagtc gtatgtgatc ttggtacaga
     8101 tcgtgtgtat acttatgatg tgtccgaaaa cggcaaattg aatcttgtaa ctaccttcac
     8161 tgcagagcca ggaacaggcc ctcgccatct tgtctttcat ccaaatggac aattcgctta
     8221 tttatttggt gaattagaca gcacagtccg cgtgctttct tataataaag aaacaggtgc
     8281 ttttgaagaa ttacagaaaa tatcgacctt gccagaagaa ttcgacggtg aaaatggagg
     8341 ggcagctatc cgcatttcaa atgatggcaa gttcctttat gcttctaacc gaggacataa
     8401 ctcgattgct gtattcgcag tttttgaaga tggttcccac attcaaaaca agcaaatcat
     8461 ctcaacagaa ggggattttc cccgcgattt cgcactagat ccaagcaata attttgtcgt
     8521 gtgtgccaat cagaacaccg ataatctgac attatatcgc cgtcatcctg aaacaggatt
     8581 actggaatta ctgaaaaaag atgtctatgt accagaatgt gtctgtgttt attttgatcg
     8641 ttagtaagaa aaggagaatg aaatgaatca gcaagacgta ttagacagac tacgggaaga
     8701 actaaatatt cctttctttg atgggaaaat cgaagataaa gagtatagcg aagaggaata
     8761 tcagaaactc aaagccgatt tacagaacta ctttgaacaa tatgtacaga atgtagaaaa
     8821 ctaatcagct ttccatacta tctgaaaaga acatcaggac aagacttttc cgtctatcct
     8881 gatgttcttt tctacaaata aaaaaccgcc gagatggtta gactcgacgg aaaaggagta
     8941 tttactcgat tttgagtaag tacaaggttg ttgttggtta taggtatgtc attatcataa
     9001 aggattttga tcactctatc ataaaggtaa tattacattt atattagata actctagttt
     9061 ttattgttat caaagatttc ttttattttt tcaacaacaa attagtcatt ttttggatgg
     9121 cagctgtcaa ttggtctgtc gcttggtctt gttttgcttc agatgtattg aatagatctt
     9181 catctacccc tgattcaaga tcgatacttt ggatcatttc ttcacagcct gctacatatg
     9241 aaagataagc tttttcaaaa tttttatgaa tgcccattgc ttttgctggt ggacgcaaag
     9301 tactgacttt cataagcatt gcccgatatt tttttgttcc ttcttcaaat aacaggatga
     9361 tctcttcaag agtcgatttg tccagttcac ttgtttttcc ttcatcaatt gcgtttctga
     9421 tcacttcata tttcggatgc attccttctc cgattgcttc tgtttccttg acgatttgat
     9481 cgatggactg cacataatag ccaatattcg gtaacatgtt cgttttccta ctttctccat
     9541 tgttgttatt atttattcta cttgttttta tcctcaaatg acaagtactt gaaaaaattt
     9601 aatggtttct atacagctcg ccattcttat tcgtgctcaa agaaaccatc tgctaaacta
     9661 tattgtttcc ctgcgacaat gtcatattgg atcaattgat attcgtcgta aatctcttgt
     9721 tgcgagcgat tgaattgcgc ttctttatac cactgtccgt tttgatagta cagttctcgt
     9781 tcaaacgccg gaagttcctg ttctaatgat aaaagcagct gataaaagcc atttgttgtc
     9841 tgattcgtct gttccattag attcgctgca aaataaaacg gactggtgat tgcatcgtga
     9901 gtcgtttgct tttctaattc tcctttactg tcgaacataa gaaattgtgt ttcatgcaat
     9961 gtttgtttgt cattttttgc ttggatcgta tctgaataga tccctggaag atgatccccc
    10021 caaaaaacaa ctaaagtcct tctagaaaga ttcttcaatt cttccgtgaa ctgcttcagt
    10081 gccgtgctgc tataggaaat atcttgcaga taattctcta a
//
LOCUS       DACCKU010000099        10121 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.102, whole genome shotgun sequence.
ACCESSION   DACCKU010000099 DACCKU010000000
VERSION     DACCKU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 10121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.102"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            36..1244
                     /locus_tag="IVN13_002423"
                     /pseudo
     CDS             36..1244
                     /locus_tag="IVN13_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
     gene            1244..1654
                     /locus_tag="IVN13_002424"
                     /pseudo
     CDS             1244..1654
                     /locus_tag="IVN13_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
     gene            1756..2055
                     /locus_tag="IVN13_002425"
     CDS             1756..2055
                     /locus_tag="IVN13_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP7012875.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            2045..3721
                     /gene="mdcD"
                     /locus_tag="IVN13_002426"
     CDS             2045..3721
                     /gene="mdcD"
                     /locus_tag="IVN13_002426"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP7012876.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            3725..4318
                     /locus_tag="IVN13_002427"
     CDS             3725..4318
                     /locus_tag="IVN13_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP7012877.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            4342..5418
                     /locus_tag="IVN13_002428"
     CDS             4342..5418
                     /locus_tag="IVN13_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP7012878.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            5443..6312
                     /gene="mdcB"
                     /locus_tag="IVN13_002429"
     CDS             5443..6312
                     /gene="mdcB"
                     /locus_tag="IVN13_002429"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP7012879.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            6423..7187
                     /locus_tag="IVN13_002430"
     CDS             6423..7187
                     /locus_tag="IVN13_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP7012880.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            7676..9769
                     /locus_tag="IVN13_002431"
     CDS             7676..9769
                     /locus_tag="IVN13_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP7012881.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            9794..10096
                     /locus_tag="IVN13_002432"
     CDS             9794..10096
                     /locus_tag="IVN13_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7012882.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
BASE COUNT         3388 a         1731 c         2102 g         2900 t
ORIGIN      
        1 tttcaaataa cttaatatta agttatgttc tatatatttg gttattaaat atttgtacga
       61 ttctgattag tttttattat ggagaaaagt tttttaaaaa gatagatgct gcttttttat
      121 ttagttgttt atatactttt gcaacttatc gaacagttga tatctattat cgatccgcaa
      181 ttgcagaagc aatcgctctt acgattattc cagctgtttt atattatgct tatggattga
      241 tattcaatag agaaaaaaaa gtcatcgctt tatctgtcag tataagtcta cttgtgtata
      301 ctcatgtctt aactacacta ttgtgtagtg cattgattgc ttgtatgatt atagttcgtt
      361 tgtttttaag caagcaaata aaaaacgata taaaatttgt ggtcgtgaat ttagtaaagg
      421 ctgttttttt gacttgtgca ttaactagct atttttggta tcctatgttg cagcagatga
      481 attatcaaga aatacacagt cccttcaaaa cagatttgca gctagaagca ctttctattt
      541 cagatagtat cattcaagcg ttaaataacg atattacaac atacactatg gggttattag
      601 gattggttgc gttgattttt cctttattca tactgaaaga aatggagaaa aaagaaaaaa
      661 tcatctatgt tggcgtagtt tctacatggg tatgcgtaac taatttattt ccatggtttt
      721 tgtttcaaaa aacaccgata agcttgctcc agtttccttg gcgtatctta ggtatccaaa
      781 tctttttttc ttcaatgatc ataacgcttg tttttatgaa aaagactatg aataaaaaat
      841 attcatggtt aatcattggc gctactgtgg tgtttctact cgtcacgtct atagcagcaa
      901 aagaaaatta cgcaaaagtt atccaatcaa aacacggtca tattgttatc aatgaagaaa
      961 cactcccacg ttatacaaca tcagatatgg gaggattata cgattacgcc ccaacagaag
     1021 cgataaaaga aaggcctcac ttagaaaaac atgaagtaaa agtaggagaa tcgtgggaaa
     1081 aagggaagta taaagctgca gataataaca ttacttacac agtggcaagc aacaaaaagc
     1141 aaaaagtgac agtaccagta tatgcttatt taggtacaca ggtaaaagta aacggtaaag
     1201 tcgtaaatga ctcttataaa aaacatggct tagtgaatgt ggaatggttg aaacatatgg
     1261 ttcaaataaa aaaccagtct ttgtcgaaga attagatgca ctagctgttc aaaaacaagc
     1321 aggcttagcc aatgcaccag tgatgattta tagcgaagat actacacata tcgtgacaga
     1381 agaaggaatt gcttatttgt acaaagcaaa aacaaaagaa gaacgacaag cagcaattgc
     1441 agcaattggc ggtgttacac ctcttggaat gactagcacg aaagaatcac tagatagctt
     1501 gcgtgcagcc ggcatcgtga aattaccaga agatctaggc atcaaacgta gtgaagcgaa
     1561 acgttcatta ttagctgcac aaaccatcga tgatttgatc gaatggtcag atggcctgta
     1621 tgaaccacca atgaaattca gaacttggtc ataaaattgg cgagaaaaac cttttgtcgc
     1681 catttaccca gatggaagaa gaaagacgaa taagcagaac gtgtatgctc tgttttacaa
     1741 aaaggggaga gatccatgga acaattagct tataaatatc caacgaaaca gccgatcaat
     1801 aagaaaatcc atgtaggagt ggtcggttca ggagatttgg aaatcttgat gtttccaaca
     1861 gaaaaaacag aatctactgt aaaaatttgt acaggaagcg atggcttcgg cgaagtatgg
     1921 aacaatgtat taacgagatt ttttgatcgt tatccaattt cagcagatat cgtgatcaat
     1981 gacttcggtg ctacaccagg agttgtgtac ctacgattga cgcaagcaat ggaggaactt
     2041 tcagatgaaa aatagttttg tagaattaaa cgccagagaa cgagtgaacg ctctacttga
     2101 caatggacgt gaattgattg atccattcga tcaaatcatc gcgccaagtt taccagcaca
     2161 aggaatcgtc cctgaaagtg atgacgggat cgttgtcagc cgtggcacga tcaatggcaa
     2221 acaagcagta gtaatcgcta tggaaggaaa attccaaggt ggcggtatcg gtgaagtttc
     2281 cggtgcaaaa atcgttgctg cacttgaaca tgtgttagaa gaaaataaaa aaggaaatga
     2341 gatctttcca gtcattgtac tagatacagg tggtgttcgt ttgcaagaag caaactacgg
     2401 actgttatct atttcagaaa tcggaaatgt aatcattgct ctaaaacaat atgtaccagt
     2461 catcggtttg attccaggac gtgtaggatc atttggcggt atgtcgatta cttcagcatt
     2521 aatgagttat ttaatcgcaa caaaaaaagc gagagtcggt ttgaatggac cagaagttat
     2581 tgaacaagaa gcaggcgttc gagaatttga ttctagtgac aaagacttga tttggaatac
     2641 attaggatct cgtcaacggg tagaatcaaa aatcattgat gaattagtga ctgatagtgt
     2701 agaagcaatc aaagctgctg tcatcgcagc aatggacgaa cgaaaagaca cgcatcgcag
     2761 tgaaagctca gcattttatt tatcattact tgattcttta gatctttcaa aaccaatggc
     2821 cattgacgat tacaatgaag caattgctaa ccatcaagca aaatcaattg acttaccaga
     2881 agtagcagaa ggaaatgaac cagctgcagc aagtgttggt tacgattggt ttgcggcact
     2941 aactggcatg aaaaatccaa cttcttctat tagtactgtg tattatggta aatcaagtaa
     3001 attcatagaa gaagccatcg tgacatcaat cgtacctgat cctaaaaacc cgatgtaccg
     3061 tgtacgaaac ggtgaagttg gtttagttga aggtttccgc atgggacaaa ttttggatca
     3121 cgtttacgaa gaagataaag acaaaaaagt gaaacggcca atcatcgttg tgatcgatgt
     3181 accaagccaa gcatacggct ataacgaaga attgatcggt atccatgtgg ctttggctaa
     3241 tagtgctgct gcttatgcaa aactccgtca agctggacat ccagttattg gattgattgc
     3301 aggaaatgct atttcaggtg catttttagc acatggatta caatcaagcc gcttgattgc
     3361 tttaaacaat aaagaaatta ccgtccaagc gatgtctaaa gaaagtgctg cacgtgtgac
     3421 aaaacgttcg gttgctgaaa tcgaagcagc tgctgaaaaa gttccagcta tcgcttacga
     3481 tatcaataat tatacaaaac taggagcgat ctatcatttc ctagaagata tcaccgacca
     3541 agctgcttca gaacaaaatg tcagcaaagt catcgaagca atcaatgaag ccattcaaga
     3601 tgtccgcaat acaaatgaca cgatgctgac aaatcgatac acaaacgaaa cagccaaaac
     3661 attcggacgt gtcgaaacaa ataaagtccg tgccaaaatg gacgaagaat gggatgcata
     3721 aaaaatgatt caagcacatg atattgtcct ttttgataaa catgaaatag aagggttcac
     3781 cttgcctgaa tgggtcgttc taactgaaaa gatgttcgga acagttcgtc gtgcgtcgac
     3841 ggatcacgca gaaatactac cgattggttt acgtggacct gcccgcaatc agagatggtc
     3901 aacagaaatc tcaaaacatg ccgtcaaaca agtcattcat ccgtgggaca tcatcgaaca
     3961 agaatcgtat agacaagaga aaatcatgga ttatcctgtt tatcagcaat ttcatgctgc
     4021 tagaaacata ttatcaggat gtaaatgggg aatcggcggc agcttaggat ttgaattatc
     4081 gactgggatt ccggcagtaa aagaaaccag tgattttgat ttgctgctgt acgcaaattc
     4141 accgattgaa ttgccaattc aggtaattca atcgcatcct gctttttttg agcaattcga
     4201 tacccaagta gtgactcggc aaggcggttt ttcactaaaa gagtatttga gagagccaga
     4261 gaaaaaaatt ctattgaaaa cagtcgaagg acctaaatta accaaagaac tttggtaata
     4321 aagaaaaaga gagagaaaga gatgtttgaa aacaaaaaaa tcaaaatcaa cgtaagtgac
     4381 ttccagaaag tatcagctgt aacggctgtt atgatgcaaa cgatattgag ctttgctttg
     4441 gcaaacagcc acagcgcaac aaatacagct tttcttggta ccttttacgt catcgttaaa
     4501 tacacagcac caatgtttat ttttgcaatt gtttataata tggtaaaaac aagtcagcat
     4561 ttgtcttatt gggaattttt gaaagaaaag ttttttgaac tagtcgttcc gtatcttcta
     4621 tggacgacgg cttatctttg gctatttcca gcagtccaac aacaaactcc gtatacggat
     4681 actgcaagct ttttactgaa atatgtcaca ggtgatggag cgcctcattt atggtatacc
     4741 gttatgatgc tgcagatcca attgttcatg ccgttttttg tttggctcgg ttacaaagta
     4801 tttgctaata aaaaatatgt atggccagta ttgattggtt caacgatttt atatgtcgca
     4861 tggtatctgt tctatcaaac acaagtattg actgggcctt atcatgacag ctggtactta
     4921 ttagatcggt ttgttgctag tttcatgatt tatggggtag ccgcatttgt ttatcacgaa
     4981 aaagtctatc aatatttgga cagagtacgt tatcttttct tacctgttgc cttagttatc
     5041 gcgttctttt ctgtccgttc attgctcgca catccaggag acttgagctt tgcgaatgca
     5101 ccttacttga atacgatcca aagtttatac agtttagtca tcatctttgc tgtcttcata
     5161 ggcgcttcca aaatgattgt aaacgattcg ccaaaattgc cattgttcaa atggttgtct
     5221 gtttatgctt atcgtacgta tttagccaat gtatttgttt tccaagtact gttattattg
     5281 tttaaagata gttggctgca gttaccttca ggagtcatga ttcttgtagc ttatctaatg
     5341 acggcaagtt gtgcatttgc tttaagctgg ttacttcata tcatttgggt agctatcaaa
     5401 aaaggtttca gcaagtaata gtaggaagaa ggagtgagtg tcatgataaa tgacgaacga
     5461 aaaaaagcag tgactcagca actcgctgcg ttggtagagg aagcattgat cgcagaagta
     5521 acattaagtc caaaacctgg attagtggat gcactggatg cgggtgcaca ttccgatatg
     5581 gactatgctt tatttcttaa gtctgctaag acgctaactc ctttttttga agaaatggct
     5641 caaatagcat ggcatcatgc tattgatcag gagttaagag aaagaattgc tgagattggg
     5701 cgaaaagcgg aaaaggcaat gctgattgct acaaatggca tcaataccca taaaggagcg
     5761 atttgggcaa tggggctatt gatcagtgtc attgcacaaa aaatcagtat ggaacagcct
     5821 ttttctttat cgaaaatact tgatgatgtc agcaagcttg ctgcttttcc tgatacaaaa
     5881 tatcgtcata aaaaaaccac tcatggtcat agggtgaagc aaaaatatgg cgtaaatggt
     5941 gcatatgaag aagcgatatt agggtatccg catatcaggc ttgcaattca agcagctaag
     6001 gagctagata atgattcata tgtacagaaa caaatgcata tgttgttagt tttgatgagt
     6061 tcgctagatg acacatgcgt attgcatcgc agtgatcaga aaacgttagt ggaaatgcaa
     6121 aaacttgctc atgctgctag tcaaagtgct ttaccaaatc gtccatttac agcactgttg
     6181 cacttttgtc aggaaaacca aatctctcct ggaggaagcg cagatctctt gtctgcaagc
     6241 ttgttcatgc tgaagataga ggaattatgg actgccgaca catacatttg tccgatgtta
     6301 ctcaatgact aaataagaca aaattgctaa aaaaacagtt tatgcatctg gttattgact
     6361 cttcctttta gcaaggctat cataaaaata gttttttgta ataaaagata acgaggaaga
     6421 caatgaaaaa aatcaattat ttagcaatca ttttgttagt gggcctatgt ttgtttgctc
     6481 tgccgaccca aggattcgct gtcgcgggtg gtcatggtgg aggtacatct agcggtcgaa
     6541 gttcgggcag aggttcattt ggtggtcgtt catatggggg cagttatttt ggcagtcgtt
     6601 cttacggcag agattcttat gggtattatg gtccaggtac aggtccttct agttatgagc
     6661 aaacgggatt tcttgcatta gctgttttgt cagtaggagg ggtttctgct ttttctatcg
     6721 tcaagaataa aagacaaaag aaaaggctga aagcggagat attgtcccgc atgtcaggaa
     6781 cggctaaaga aaagaaagca cagtttctgg aaatccaaaa gatattcatg aagatccaat
     6841 ccgcttggga agaagaaact cctgaaaaag ccagaagctg ttatacagac aagctgtttg
     6901 ccgaacatca gaaagttctg caaaaaaaca aagcagacgg tgtaagaaat catactaaaa
     6961 aaataactat tcaggaagta ggaaattatc gacagatcca cgaaaatagt tttgccatcc
     7021 gaattgattt cagatgctgt gattattcag tcgatcgaaa agatgggcga ttgatctcag
     7081 gaagtaagac acaacgacag tatttttctc agatatggta ttttgatttt tctgagaaag
     7141 aaaaaagatg gcaagccgat tttattcagc cgattatgct ggattgacag gaaatattta
     7201 tgaacggagt tgtgacaaaa gccttgtcgc agctcctttt tctgaataaa cggtgtgcca
     7261 gagatagcct atcggcaata aggcacgatt ttgtaaaata tgggaagcga tttttcaaaa
     7321 tcgcttctta tcgctcaagg ctgaacatct ctgtcacaac ctctccacta aatggtgttt
     7381 aaaagcttgc atctgcgata ataagaacaa ccaaacgaaa aataggaagc actattttag
     7441 tttagttgtt cttattactt gatgcaggac agctttttca caacctcttt tttgtataga
     7501 tgggcaaaat ttttgtaata ataggcgtat gtctttttcg ttgaggagat gaaaaagcat
     7561 atggcttatt ttttgtttgc tttgcttact ttctttccac ttcaagtggc agaaatatct
     7621 actttttatt atgtaagcgg aaccatatac ttgaaatcag aaggagatca tttctatgaa
     7681 aacaagaaca attgaaatac ttcacagaat catgacagcc caatatccag taaaagcaaa
     7741 acatctagca agtgagttca atgtcagtca gcggacgatc cgtcaggaag ttcttgaagc
     7801 gaatatctgg ttgagcagta aaaagcttcc ggagatccgt acaattcgca acaaaggttt
     7861 tttgttgaag ctggaaaagg aaaagcaagc tcgattagaa actgaaataa aagatgtgaa
     7921 agacggcttt ttgaatcggc gcgaacgtat ttttgatcta gttttagccg ttgcttacag
     7981 ccgaatcccg atccatctaa acaaaaaaga agaagaattt caaatctcta aaagtacatt
     8041 agatgaagat atgagacggt tacgagcaac actggtcagt tatgggatag agatcgtcag
     8101 ttatggaaag caaggactgg tttataaagg ttcagaacga tctatccgta cgatgatcta
     8161 tgattttatc aatcagaatc tgggaatgac cgacttcttt atgaatcaag atgcacaatt
     8221 aacaccggct caaaaaatct ttctccatta ttttcctttg gtggaaatca aaaaaataca
     8281 ggagatctat tccaataagt tgttgaaaca agaagatgat atttataaaa aacaaatcct
     8341 gctttttaca ttgatctgga tccagcgaat caggaaacat gaatttatct ctgccattaa
     8401 ttggaaaaat acagaaataa aagacagtag cttttctgca tttattgacc acatgatcga
     8461 ggaatttcaa ttggacaatg tcccgcaagt agaaagaaat tatcttcgtt tcactattga
     8521 gacgttcaat gctagagata tctccaattc aatcgagtgg gtacaagctc aattactaac
     8581 gatccaattg atccaatttg tagagaacga gacacacgta cctttccatc taaaggaaga
     8641 agctttattc gaaaatctct ataagcacat ggctgcactg attgtaagaa tcaagaatca
     8701 tatccaagtc atgaatccat tgaaagaaaa catccgaaca aattatttcc caatctaccg
     8761 cgcagtcact cattttgttc ctacgatcga agaagtaatt ggtggcaaga tactggagga
     8821 cgagatagct tttcttgtca tccacttttc gacgatcgcc agtgtaatca agcgagactt
     8881 gaattatgtt tataaatcag tcgttgtctg taaccatggg atggctactg gaaatctgtt
     8941 agcagagaat ctgaaagaaa agtttcctca gatcgagatt actgcagttt taagttcaaa
     9001 agaaatcgaa ttagtagata aattggatgt caatctggtt ttcagcacct ttcaattgaa
     9061 ttatcgaaat aagccgcttt tagttgtcga tccgatttta actgagacga atatcttggt
     9121 tatagaagac tttttgaaaa gaaatgaaga actacagcgg ttagagccta acagtcatga
     9181 atcgacggag ttattcactc aaataataga tgtcatgaaa gaaagtggtg gccgagtaga
     9241 tcgaatcatc tatggcaagt tagaaaaatt attcgaaaca aacaatttag aaatcaacaa
     9301 aagggagatc cagcctatgt taaaagatat tttgacggac agcaatatca tgatcaaaga
     9361 aaaagccact acttgggagg aatcgattga actagctgct agaccattgg ttaaagaaaa
     9421 cattgtcgaa gagcgctatg tcgaagcgat gattcgtgca gtgaatgatt acggaccgta
     9481 tatcgtgatt ggcaaacaca tggcactagc ccatgcccga ccagaagatg gggtcaataa
     9541 attaggagtc agcgtagcta cgattgagca accaatcgat ttcggtaatc ctgaaatgga
     9601 tccagtaaag attatttttt gtttggcagc agtagactcc tactcgcatc tgaacatcat
     9661 gaaagaacta attgaattga tcaacaacga agagaagttg gatcgattga ttgaatgcac
     9721 aaatatcgaa gatttcaaac aactgctttt ttcagtagtt gagaaataat aaatgatacg
     9781 ggggagaaca aagatgaaca aattgaacat tttattcgta tgcggtgcag gtttaggaag
     9841 tagttttgca gcacagatgt ctgctgagga tgttttgaac aaattaggag tagaagccaa
     9901 actggaccat tgtgatattt cttccgcagt ttccatgaaa ccagatgtga tcatcacagc
     9961 agaaaatttt cgttcgcagt tcgaaaagtt cgcagttgat cctaaaacgg cgatgatcta
    10021 tttgaaaaat attgtttcaa aaaatgaaat tgaagaaaaa atcaccccag ttctgcaaga
    10081 taagggagtt atctaaatac aaaaagaaaa gagggtaaaa a
//
LOCUS       DACCKU010000100         9888 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.103, whole genome shotgun sequence.
ACCESSION   DACCKU010000100 DACCKU010000000
VERSION     DACCKU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.103"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            1..357
                     /locus_tag="IVN13_002433"
     CDS             1..357
                     /locus_tag="IVN13_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012883.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
     gene            373..723
                     /locus_tag="IVN13_002434"
     CDS             373..723
                     /locus_tag="IVN13_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAP7012884.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            752..1531
                     /locus_tag="IVN13_002435"
     CDS             752..1531
                     /locus_tag="IVN13_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAP7012885.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            1542..2219
                     /locus_tag="IVN13_002436"
     CDS             1542..2219
                     /locus_tag="IVN13_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAP7012886.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            2238..3344
                     /locus_tag="IVN13_002437"
     CDS             2238..3344
                     /locus_tag="IVN13_002437"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAP7012887.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            3334..4425
                     /locus_tag="IVN13_002438"
     CDS             3334..4425
                     /locus_tag="IVN13_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAP7012888.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            4438..5178
                     /locus_tag="IVN13_002439"
     CDS             4438..5178
                     /locus_tag="IVN13_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAP7012889.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            5466..7406
                     /locus_tag="IVN13_002440"
     CDS             5466..7406
                     /locus_tag="IVN13_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP7012890.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            7493..9415
                     /locus_tag="IVN13_002441"
     CDS             7493..9415
                     /locus_tag="IVN13_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP7012891.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(9695..>9888)
                     /locus_tag="IVN13_002442"
     CDS             complement(9695..>9888)
                     /locus_tag="IVN13_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAP7012892.1"
                     /translation="ITLCGDAKDKCPAVPNNHEHLHWDLKDPAQAVGSEEEILTEFRK
                     VREGIKRKVLDLNQRTSIV"
BASE COUNT         3224 a         1639 c         2200 g         2825 t
ORIGIN      
        1 atgattaaag tagttattgc agaccgtatg ggtaaaggac aaaatgtggc caaaggggta
       61 gaagcagctg gtggaaaagc agttgtcgta ccaggaatgg gggcagatat gcgtttgggc
      121 gatgtgatgc aacaagagca tgcagatatg ggtatctctt tttgtggtag tggtggtgca
      181 ggtgcattaa cagcagcaac aaaatatggc tatccagaac gacatggtat gcgttcgatt
      241 gaagaaggga tcacagctat caaggatggc aaaactgttc taggtttcgg ctttatggat
      301 caagaggaat tagggaaacg tctgacagaa gaatttatca aaaagcatgg aaaatagaaa
      361 gagggacatg aaatgagcgt tcaaaagaca attcagcaaa aagttatcgt ctccggtaaa
      421 ggggaaacaa agcaacatgc ttttgcatca gcgttagcag aaattcaaaa caaaataatg
      481 gaacaatcaa acgtattatt gcgaatcgaa ccagtcaatg tggatatttt aaaggcggtc
      541 gaaaacaaat acacagaacg ctttctattt ttcttcttcc cacggcaacg tattagctat
      601 gaagtcgaac tctctgttac tgtggctgta acagaaataa aattggcaga catatcgttc
      661 agccagcaaa tgactgaaga tccaaatggg attgccattc catttgtaac taaaaaaatc
      721 taattttgaa tcataagggg gaaagaataa cgtggatttc gtaatcattc tagtcaaatc
      781 ggtcattatc gggggattac ttggttttgc agcatctgtc ggggcagctc gaatgtttca
      841 tgcacctaat acacaaggtt taggagcttt tagaacatta ggcgaaatga atgcctgcat
      901 gggtgatcca gcttctcatt tttcatttgg tttaggcttt ttctttaatg catgggcttc
      961 ttcagttggt gctggtgcat tcacacaaga tgtgacgcat cgaatcattc ccaattgggc
     1021 agcggcagca cttctagcaa aaaacaaaga tgtcacccag accatgcatg atccgaaaaa
     1081 aatggggatc attggagcgg ttatcggggc agtggtggta gccttcttaa acacaacagc
     1141 tgcagcaatt ccagaggcgt tacaagtgac ggcggtttcc gttttggtac cagcagcgac
     1201 gattctgatc aatacagtaa tgccagttat tttttggttg gcagctttag acgcagggaa
     1261 acgaactggt ttttggggca ctctttttgg tggtatcgcg caattgatta tgggaaatgc
     1321 ggtacctggt gttgttctag gaatcttgat tggtaaaggc gtcgatgaga gcggttggaa
     1381 tcgcgtcacg aagagcatga tgatcgctat tgttttatta tttattttca gtggattttt
     1441 ccgtggattt gatatggaaa tgatcaagag cttccagctt caagtgccta attggcttga
     1501 taacttgcac aacatcttta gtgtgaaata ggaggagaat catggagaca acagaagaac
     1561 aagcaaaagt attagaagca aagcggcaaa agagcttttg gtttgcagac tggtcttttc
     1621 cgctaatcgt aggaattatg tccgctgctg tatttgcagg aacacacatg tatgtgatgt
     1681 atggtgtggg tgcattcaat gaagtttcga tcgtggcgat gttaaaagcc gggctggatg
     1741 gcggatccta tggcgcagcg gcagcgtttg gtgctagttt cttatttgcc agaattttag
     1801 aaggctcttt agttgggatc ttggacttag gcggttcgat cctaactggg atcggaattg
     1861 gggtaccagc aattctctta agtatgaaaa tagcggcacc aatcgataat tttgcactct
     1921 ctttattgac tggtttggtt ctaggtttga ttgttggtgg catcattgtc ttgattcgta
     1981 aatttacgat caatcaatca aattccactt ttggtgctga cgtcatgatg ggggcaggaa
     2041 attcatctgg acgttttttg ggtccattaa ttatattaag cgcgtgtgca gcatctattc
     2101 caatcggcat tggcgctaca ttaggagcac taggattcta tgcttggaaa aaaccaattg
     2161 ctggaggtgc tattttagga gcaatgattt taggagcaat cttcccggca gtaaattaat
     2221 agaaagaggg tcattaaatg tttgatactt tgattaaaaa tggaaggtta gttgatggat
     2281 ctaaaataga ggtcgctata gaaaaaggca tgattaaagc agttgcagca gaaataaatc
     2341 agccggcgca agaagtagtt gacttggagg gaaagtatta tttatccgct gggtggatcg
     2401 atgatcatgt ccactgctat gaaaagatga atctttatta tgactatcct gatcagatag
     2461 gggtcgaaaa aggtgtgacg acggtcattg atgctggaac tacgggagca gaaaacatcc
     2521 gtgaatttta tcaattgacg aaatcggtca agacaaatgt gtatgcgcta atgaatatct
     2581 ctaaatgggg cattgtcaaa caagatgaac ttgctgatct aacaaagatt caagaagatc
     2641 tagtaagtac tgcgttagca gaactgccgg aatttattgt cgggattaaa gcgagaatga
     2701 gtaagacagt tgttggtgaa aatggcatta agccattaga attagctaaa aaaatccaga
     2761 cagaaaacaa tgatctgcca ttgatggttc atatcggttc tgcacctcct gagttagaag
     2821 aagtattgtc aagaatggaa agaggcgatg tattgaccca ttgttttaat ggaaaaccaa
     2881 acggtatttt agatccaaag acagatcaaa ttaaaaactt tgtctgggat gcttacaata
     2941 atggtgtcgt ctttgatatt ggacatggga cggatagctt taatttccat gtggcagaaa
     3001 ccgcactaaa agcaggaatg aaagctacct ctattagtac ggatatctac atccgcaatc
     3061 gtacaaatgg cccagttttt gatctagcaa ctactttaga aaaattgagg gtagtcggct
     3121 attcttggga agaaatcatt gaaaaagtta cagcagttcc tgcacggaat tttcatttga
     3181 agaataaggg ccagttagct gtcaattatg atgcagattt gacgatattt gagattgttg
     3241 aaaatgaaaa ggaattgaaa gattccaacg gatttacacg tatagctaaa gaacaaattt
     3301 taccgattaa aacaattatt ggaggcgtca tatatgacaa ttagctatgg aaagtttgat
     3361 ttaaaagaag tcattaatgc atcgggaaaa atgacgatcc taggggtttc taaggtatct
     3421 gaaagtgtcc tagcagcaca acgatttgga ggcgagcatt tttttgagat gagtgatctt
     3481 gccatcaaaa ccggcgctta tctttctcaa ttactgaaag tggaagatgc gcaaatcgtt
     3541 tcttctgctt cagcagggat tgctcaatct gttgctgctt taatcggaca gggcgacatg
     3601 taccatgtct atcatcccta tacagaaaaa atcgctagac gggaaatcat catacctaag
     3661 ggacataatg tagattatgg gacaccggtt gaagtaatgg ttgctcaagg tggaggaaaa
     3721 gtcatagaag ctggctatgc aaatatgtgt acaccggaac atgtttccat gatgattacg
     3781 gaaaaaactg ctgcgcttct atatattaaa agtcatcatg cagttcagaa aagtatgctg
     3841 acggtggcag aaatggtaga agttgccaaa gctcatcgtt tgccattgat cgttgatgca
     3901 gctgcggaag aagatttatt caaatatagc gaaatgggcg ttgacttagt gatttactct
     3961 ggggcaaaag caatcgaagg tccgagtgcc ggactggtca taggaaaaaa gaagtatatt
     4021 caatggattc ggctacagtc taaaggtatt ggccgagcaa tgaaaatcgg taaagataat
     4081 attttaggct ttacccaggc actagaagat tatttaaaaa atgggagcga atccggtgac
     4141 tccatgaagc aacgattagc gccttttgtt aacgcaatca atcaacttcc tgacctttca
     4201 gcgaaaatag ttcaagatgg tgccggcaga gatatttatc gagcaagtgt aacgattaat
     4261 ggcgataaaa gtgcgaaaga agtcattcag gaattaaaag cacgaaatcc agcagtctac
     4321 acccgagaat atcaagcaaa taacggaatc attgagttcg acattcgttc cgtcagccaa
     4381 aacgaaatgg aaaaaattat aaaaagattg agagaaatca tgtaggaggc ttttaaattg
     4441 tcagttatcc caaattatta taaaaatcgt gtatgtttaa atgtattagc tcattcagtt
     4501 gaaaatgcaa aagcatgcta tgaagcagcg gaagggcatg tcgtactagg tgtgctatcg
     4561 aaaaattatg ctagcgatga agcagcaatc gaagatatga aaaggtacca agacgcaacc
     4621 aataatgcgt tgtcagttgg attaggcgct ggagatccaa accaaagtca gatggtttcg
     4681 cgtttatctg gggttttaca accacaacat gtcaatcaag tatttactgg cgtggggact
     4741 tctcgtgctt tattgggcca aaatgataca gtcatcaatg gattggtttc accaacaggt
     4801 aagatcggct atgtcaatat tgctacaggt ccgttaagtt cacaagcgcc agcagctgaa
     4861 gtgcctattg aaactgccat tcaaatgctg caagacatgg gaggtacttc aatcaagtat
     4921 ttcccgatga aaggcttagc acatagggaa gaatttaaag cagttgcgga agcttgcgca
     4981 aaatatgaat tctatttaga gccaacagga ggaattgatc tttcaaactt tgaagaaatt
     5041 ctgaaaattg caatcgatgc aggtgtaaaa aaagtgattc ctcatgttta tagttcaatt
     5101 atagataaag aaacaggaga tacgcgtata ggagacgtaa agatactatt aagcatggta
     5161 aaaaaaatta ttcactaact cattagaaaa taagaagctt attccagaat gaaattaaat
     5221 ggaataagct ttttataatt aattacggaa attgttaatg tagataataa tttagatgta
     5281 cgtaagcaga agtacagaaa tgtaagattt tccaatatta ttttattcaa cataataaat
     5341 cgtatatcaa tttttttagc gaaatttcta tttattccaa gaatttcagt tattacggaa
     5401 tcattttgtg cttaataaaa agaaaccgtt tttatatact ataaatgtag aaaggcggta
     5461 ggaaaatgaa aaaaattatt gcaatcacat cctgtccagt tggtattgcc catacataca
     5521 tggcagcaga aaacttagag aaagcgggga aagcagttgg cgccgaggta aaagtcgaaa
     5581 cacatggttc catcggtatt gaaaatgaat tgactgcaag agatatcgaa gaagcagctg
     5641 gtgtaatcat cgcagcagat actaaaatag ataaatcacg attcggcggt aaacctttga
     5701 taaccgtagg tgtgcaagaa ggaatccacc atgcagatga gttggtgaga gatattttag
     5761 caggaaaagc gccagtctat aaaagtacgg aggcaattat ttcttccgaa aataaaagtg
     5821 aatcagaaaa tttaggtaaa aaaatctata agagtttgat gaatggcgtt tcgtacatgg
     5881 tacctttcgt agttacagga gggttattga tcgcgatttc tttaacatta ggaggaacag
     5941 ctacacctga agggatcaag ataccagaag gaacgatttg ggcaacgatg aatagtatcg
     6001 gaagtatcgc tatggggttg atggttccaa ttctatctgc atttattgcg caaagtattg
     6061 cagatcgtcc aggacttgtt cctggatttg ttggtgggat gttagcggca aacggagcat
     6121 tatatggtag tgatgcgaat gcgggattct taggcgggat catcactgga tttttagcag
     6181 gattcattgc tttaggaatc aagaaagtgc gagtaccgaa agctttacaa tcgatcatgc
     6241 caattattgt tatcccaatt ttaggatcat tggcagttgg ttttgtgttt atctatgtta
     6301 ttggagaacc agtagctctt ctatttagtt cattgactca ctttttagca gggatgcaag
     6361 gaggaagtga aattgttcta gctatgatct taggagcgat gattgctttt gatatggggg
     6421 ggccgttcaa taaagtagcc tttttgttcg gatctggctt gattgcagaa ggcaattatt
     6481 ccattatggg gccaattgct gtagctatct gtatccctcc attagcattg gggctggcat
     6541 cattgatttt aaaaaccaaa tttacgaaaa cagaacgaga agctggtaag gcgtccttag
     6601 caatgggact gtttgggatt acagaaggcg ctattccgtt tgcttcaaca gatccactac
     6661 gggtgattcc aagcattgta atcggggcaa tgacaggaag tgtgatcgcg atgctttcag
     6721 gagtgacaga tcatgtagct catggcggtc cgatcgtagc ggttttagga gcagtggatc
     6781 acgtagccat gttttttgta gcagtgattg ctggtattgc tgtaaccgtg ctaatgttaa
     6841 gaatccttaa accgacatta caacaaactg cggttgctgc agcaaatggt gtgacagtag
     6901 cagatctatc agagactgaa acaagtgatg tttatacgaa aactgaaact tttgattcta
     6961 tcacggatat tatgtcagat gaagcaatcg ttttaggtga aacagcaaca acaaaagacg
     7021 gagtaatcga agatttagtc caacgtttag aaaaaacagc ggcgatcaca gataaaacca
     7081 gatttaaaga agtcatctat gatagagaac atgaaagtac aacgggaatc ggtatgggga
     7141 ttgctattcc acatggaaaa tcagcttatg ttcttaaacc aacagtggca tttgccagaa
     7201 gtcaatctgg ggtagattgg cattctttag atggaaaacc tgcacatatg atctttatga
     7261 ttgctgtacc tgaaaacagc caaggtgata tgcacttgaa aatactgcaa cgtctatcac
     7321 gtaaattgat ggatgatgac ttccgtcaag cactgatgga agcgccagat aaaacaacag
     7381 ttcaccaatt attaagcgaa atgtaacata agatgaaaaa tcgaccgtga tgagacataa
     7441 tagatgtgtt atcatgatcg attttttatt tagaagggag tttctgcaca ttatgaatca
     7501 gcgtcagcaa tctttgatct atcaattaag tgagaacaat aattatataa caacaaaaaa
     7561 gctagcagag catttccatg tgtcagaaag aacgattcat agcgatttga ataccattga
     7621 aacttggttg agagacaaac atctgccttt aatgtttgaa agaaaaaaag gtacaggtat
     7681 cttgttgaat ggtctgcctc acgaaaggga acaactgaaa cgagcgctta atgaagagac
     7741 tcaaacagaa atggataaaa aacagcttcg tcagttattg attttccatc tgctagtagc
     7801 aaaagacgga tttagtcttg aagaactttc agaaaaacta tatgttggaa aacgtacgat
     7861 tcgtaaagaa ttgacagagt tgcaaagctt ttttgagtat caccatttac agctagtatc
     7921 caaaacaaaa ctaggaactt ttcttaaagg cgatgaacaa gaaaaaagac agttgcttgt
     7981 taagacatta cgaaatatgc agaaagaaga tccacaaagt ccgtcgttga aagaattctt
     8041 cgctaaagat acattaaaag ttatccaaca tacattacaa gatgtttttt atgaaaacca
     8101 tttagaacag ccgaatggac tggctagtgt ggagattcac atctacttta tgctcgagcg
     8161 aatgaaacag taccagaaag tgaaattaag taaagacgaa aacgaagtcg tggaacacac
     8221 ccaagcacaa aagttaagta gtcaaatatt agcaaaacta gccaccatct atccaattga
     8281 attttctcct gatgaaatca attatctggc tttgcgtatc gccaatcttt ttacaaattc
     8341 acaagcagcc acacgttttc aaaaggaatc tagcgcactt gctgatcatt tgatcgaaca
     8401 agtggaacag tttctaggct attcattaaa agaagatcag ctactaaaac aaaatttaag
     8461 gtctcattta tcttcaactt attttcgact ccattatggt ttgcagattt ctaatccatt
     8521 gacgaaaaat gtgtttagta cgtacacaca acttttctta gttttacagt taattttaga
     8581 agattatttt gaaaaggaaa atttttatgt tccacaagag gagacagctt atttaacgat
     8641 ccatttccaa gcagcgatcg agcgacacaa acatcagaaa agtagaagat atcaaacggt
     8701 tttagttagt gaatattcaa aagctatggc tacttttctc gaagcacggc tgaacagaga
     8761 acttccagaa ttaacgatta tggatctagt tgaatataag ccagaattaa agagacaaga
     8821 ttttccctct gttgatttta tcttggctac gttaccattt aaacatataa gcattccagt
     8881 aatcgagatc tcaccaatga tcacagaaac tgatctagcc tatttgacaa aatatatgct
     8941 ggagcatgtg cctatcaaga agaaaaaaac atttgatcta gcaagcttta ctcatccatt
     9001 tctgatcttt cctcaacttg aacggacaga cccagccgat attttaaact ttatgggaaa
     9061 cgtcttagtg gaacatcatt atgtggagcc ggaattcatc gattctgtct tggaaagaga
     9121 cagtcactcc agtactagag tagcaccatt tgtaacaatc cctcacggta atccgctgta
     9181 tgtgaagcat tcgatgattt ctattgctac aatgaaagaa ccagtcctat ggcacgggga
     9241 acagatacgt atcgtactgc tattagcaat aaagaaaaat gatttgaaac atgcagagtt
     9301 caaaaaaata ttttctgtta ttcactatct agaaaagcag ccagaacaaa tggctcagtt
     9361 gatgcaatcc aatcatccgc tagaaatctt aacgttgtta tctagttatg aataagtagc
     9421 gattaaaaaa ctgcctttat atttataata attctatcta tttatttgga agtagaaaac
     9481 taaatacaaa aaaagaggat gtggccaaag ttgtggcaca tccttttatt ttgagtttct
     9541 tataattttt tatagttgct tacgatgtct tgactactgg taaacggtgt tcaagagaca
     9601 atttgtcggc aataaggcac aattttgtaa aatatggaaa gctatttttt taacattgtt
     9661 tcttagtgct tgaaaaatca ttttgaatac ttctttatac gatcgatgta cgttgattta
     9721 agtctaaaac ttttcttttg attccttctc tgacttttct gaattccgtt aatatttctt
     9781 cttcgctccc gacagcttgt gcaggatctt ttaggtccca gtgaaggtgt tcgtgattgt
     9841 ttggaacggc agggcacttg tctttggcat caccacaaag tgtaatga
//
LOCUS       DACCKU010000101         9835 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.104, whole genome shotgun sequence.
ACCESSION   DACCKU010000101 DACCKU010000000
VERSION     DACCKU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.104"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..233)
                     /locus_tag="IVN13_002443"
     CDS             complement(<1..233)
                     /locus_tag="IVN13_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7012893.1"
                     /translation="MSYTGILSFEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPT"
     gene            complement(1069..2109)
                     /locus_tag="IVN13_002444"
     CDS             complement(1069..2109)
                     /locus_tag="IVN13_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP7012894.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2458..2784)
                     /locus_tag="IVN13_002445"
     CDS             complement(2458..2784)
                     /locus_tag="IVN13_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012895.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2771..3559)
                     /locus_tag="IVN13_002446"
     CDS             complement(2771..3559)
                     /locus_tag="IVN13_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7012896.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3927..4217)
                     /locus_tag="IVN13_002447"
     CDS             complement(3927..4217)
                     /locus_tag="IVN13_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7012897.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(4353..4532)
                     /locus_tag="IVN13_002448"
     CDS             complement(4353..4532)
                     /locus_tag="IVN13_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012898.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4529..4879)
                     /locus_tag="IVN13_002449"
     CDS             complement(4529..4879)
                     /locus_tag="IVN13_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012899.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4872..6197)
                     /locus_tag="IVN13_002450"
     CDS             complement(4872..6197)
                     /locus_tag="IVN13_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP7012900.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            complement(6568..7152)
                     /locus_tag="IVN13_002451"
     CDS             complement(6568..7152)
                     /locus_tag="IVN13_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7012901.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(7363..7566)
                     /locus_tag="IVN13_002452"
     CDS             complement(7363..7566)
                     /locus_tag="IVN13_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012902.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(7576..7998)
                     /locus_tag="IVN13_002453"
     CDS             complement(7576..7998)
                     /locus_tag="IVN13_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012903.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            8312..8926
                     /locus_tag="IVN13_002454"
     CDS             8312..8926
                     /locus_tag="IVN13_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7012904.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(8950..9300)
                     /locus_tag="IVN13_002455"
     CDS             complement(8950..9300)
                     /locus_tag="IVN13_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7012905.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(9294..9557)
                     /locus_tag="IVN13_002456"
     CDS             complement(9294..9557)
                     /locus_tag="IVN13_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP7012906.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT         3067 a         1754 c         1431 g         3583 t
ORIGIN      
        1 gttggatata gctcttttaa agggatagga tcctttaaaa ttatttgctt ttgtttccca
       61 atgtaataaa tcatttcttc tgagactttg tctttctgga ttatatattt cttacactgc
      121 acaacggttt tattttggcc agttgatagt ttcttcgtga ttttctctgt agcggtacac
      181 cgtttaccgt aatgacaaat atcttcgaaa ctcaaaattc ctgtatatga cataaagtaa
      241 tcctctcact ttctttaaat atttttaata agtttttctt gttatttttc aaaaatagag
      301 aattgatttt cgttcatgac aaacaaaaaa gagggaacgc attcaaacat gcgttccctc
      361 taatttcagc gttcgtcata cggcaaagag ccacacggac atttttataa cattatgctt
      421 aggcatagat tcattacaat atcacgtatt tttaacctta atagagccaa aaaaagtggt
      481 atcacttttg aatatctgtt atactgagtc cgttgcgggt aataccgtaa cactgaaggg
      541 cacgcattaa ggcgtcatct taatgctttc ctgtaggaca ctgacggaga ttaccagtct
      601 cccgagcctt acaggggcct ttcttttttt tgtattttca attatcacaa tttatcgtcc
      661 aaaagtgcag aacgaaaatc aattcatcac aagcatatca gacaattaaa tttaaaacaa
      721 ctaaaaatat acaaaaaagt gtaattgaat attagcagca ctgcctgaaa agaagtaata
      781 tcaggctttt cagcttgggg aaataagtga attacctctc ttcatgaatt attataactc
      841 aaaaaagcac atatgcaaac actagttccc tatttttttg agtgaaaaat aactaaaata
      901 tctaacgatt atgaatttaa aacctacgat tgtgattgtt ttcataaact tttaatctaa
      961 aaactccgga atactaacta tttatttgta atatttgtaa taaaattaaa atcataaaaa
     1021 agttctaatt taaaattgat catttttata gtaattgctt tttatcaact actctaacca
     1081 attttccgta ctaactgttg gtaattcgtt aaatgtttct ctttgttcgt ttgcaagctt
     1141 tgccgcaata gctttttcga caaaccaatt ttttacataa acgaaaatta gattctgcaa
     1201 attgtttact ttttcagttt tttgtttttg ataagctttc agtaaagtca tatataatcc
     1261 tttgtcagca tcaattccat aagattccag ttcttcataa acgacaatag tttgctctaa
     1321 ttcttcagct tttttctttg cattatgaat agtttggacg gtttgttgag cttcactgta
     1381 acttgaagaa aatgttttta tcaagtttaa tacattttct ggtacaaatg tatttatact
     1441 gtcctcttgc atgatgtcga cgaagttatc tagcaaaact tgattttgta actggtcagt
     1501 ttcagtgtct attatgtgtc tattagtgtc tatagaatta gaatagaata gattgtctgc
     1561 gattttcgca attacggttg agtccttgat atgactggat tttgccgtat ttgcgatttt
     1621 cgcaattacg gtagaatcat tgatttttcg cggttttgcc gtatttgcga ttttcgcaat
     1681 tacggtggaa tcagtattgt cttcctcttc catgatttta aagacgtctt gattcgtaac
     1741 ttctggcttg cctaagtaca ataaattagg catagattca atcttaccat cacgtttatt
     1801 tactcttcct ctcttctgtt tcagaagacc tgctttttct aattcttttt tgatcttaga
     1861 tactttacct tcgcggcatt gtaacaattt cattaattct tcaactgtga atatgaagaa
     1921 tatattatca tcagaatcta cccagttatt tttgactgaa taatcaaatc gatctttaag
     1981 tagcatataa gcaatttttg catcatttga aagatccatg tatttttcgt tagtaaaaaa
     2041 taccttaggc atttgataat atttttcacg ataaatttct tttgatttgt aataattaaa
     2101 atcattcata gccaatcctc tttctgtagg atagcgtttt gttcaaaact atgttacaat
     2161 gaattaaaca aatatatcta tccactatat atttttctac tgctgataag tcgccaaact
     2221 ttgttaaagc agtagtcttt ttttatcttt ttttagttta ccttaaaaaa taaattaaag
     2281 caaaccgctc tatttcaggg tttaagctca tggtaactat atcacgatat tacaataaca
     2341 ttatatcacg atatcgtaat attacaatat gaagataagc gacttatcag ctttgatact
     2401 actgattttt ataaaaaaaa tatcacgata ttatgatatc gtgatattga atgtaaatta
     2461 aattttattt tgtttctttg ctagtttata tccttcaatc aagtattctt caggtatgga
     2521 ttttaatagc gcatcttcta taacgtctgg tttacttttg tctgattcag caatagtcgt
     2581 ccaatatttg agtgctgaca ttagaattgg atttatacga atgctatctg ctgttcttac
     2641 actacttcct tgatctttta tgactttact tatatttttc ttggttaatt cgatttcttc
     2701 tacttctttt ggtttgtagt tctttttatt tttatttcct agtagtctac ttactgcagc
     2761 ttgactgcga ttatcttctg ccatcaaaat caccaacttc ttttaaatat tcttctgtaa
     2821 atgctttata gtcatcttca gctgttgagg gtttattaaa attgattaga tctgatccag
     2881 cagcttgcgc ttcgtcaacg gttattgttc tacgaatata agttttgaaa atttttgcac
     2941 caatgactct agaaatttcg gttgctgcag tcgtcatttc tttagaaatg ttataacgct
     3001 tattatactt agtaaatagg actcctcgta cttcaagctt gtcgttcgta tattctttta
     3061 ccgtttcaat agtttcactt aactttatta gtccttttaa tgagaaatac gcagcttcaa
     3121 ctggaatgat aatttcatca gaaacagtaa atgcgttgac tgtaagcatt cctaaacttg
     3181 gtggacaatc aattataata taatcatagt tgtccttgat tggttgaagt actcttttaa
     3241 gcctgtgttc acttcctact tgagtaaatt ccctctctgc agaagaaagg gctatgtcgg
     3301 ctggcaaaac atcaattcct ttatagtcca gaatagcatc tgtaatgtca gcctcccctt
     3361 ttaatacttc ataaattgta ggttggtttg ttccatctat atcgaaacct acattatctg
     3421 tagcatttcc ctgaggatca atgtccacta aaagtacatt tttaccgtaa acaaatttca
     3481 aacatgctgc taaattaacg ctagtcgtag ttttagaaac tccacctttt tgattggcta
     3541 ctgacaatat tcttgccatt aaaatatccc ccattcatct tatattataa tataattata
     3601 tcataatatt gtgatattgc aatatcgtat atgcattata ttgtaatatt gtgtgattat
     3661 tttgttttaa agatatatat cggttcggat ataaagatta ttcatatgtt attcgaaagt
     3721 aaaataatca ctttttatta ctttaaaatt ttttattaat cataagaaat atgttttgcg
     3781 agcagcaact agaataattt aaacattatt aaactgtcta tattgttact aataattccc
     3841 ttcttatagt gcattttctt agaaaaaaac aatattacga tatcatgata ttaagatatc
     3901 gtaatattat acaattgtga ttgatattat atatgttttt ttattttttt atcatgttta
     3961 atcttgaagg aatcatattc tttttctaaa gacaggagct tttcataaac atcagacatg
     4021 tttaaactag aggccaggct taaatcataa acgaatgaag caggtaatga ttttattcta
     4081 cgttgtcggg ttatccagct tgcaaccgtt ccttgtgggt aggcatataa ctcacaaaat
     4141 tgttctacag tgattcctaa attcttgata atgtaaacat tgatcgggtg cgagtaaaca
     4201 aatgtattga tagacatggt gcgtaagcct actttctttt attattgcgt ttgcaataat
     4261 agtttaacac acgcattatt ctagtatcta ctcctatttg taaaaacaaa aaaggattca
     4321 tagagataac tctatagaat ccgtaaaata ccttatatat ataacgggaa aatataaaac
     4381 ctaaaattat gataacctgc tgcagcttct acatagaaag agaagcgctc aacaccaacc
     4441 tcatttatca aacgttggcc ataaccccaa atctcttctg atagctgatc aaaggttata
     4501 tactttagct catattgctg gataatgttc attctaatcg actccattta ttatcctggt
     4561 aaaattcgat atgacgtatt tcgtcgtagt gaattttgtc ttttccaacg tagatcccca
     4621 gctcatcact acctaagatc ttgccaacaa tatcttctgg atagtatcct tcagcgtttc
     4681 gttcttcttt ttgaatagat actgatctat tttttatgac tgcagcgttc aatacttccg
     4741 caatctcttc agtcgtcatt tgttcttttg ctaaattttg actggttcgt tgttcattgt
     4801 cggcatcaat ttcttcagta tgatcagata aataaaaccc tgcccacttt aaacgtccac
     4861 gatcattgta atcaatcatt tttaattccc ccatttccgc ccgcatgacc accaacaagt
     4921 gtgcttctag taatgcttct agcgctttct aacattgaag aagcatgtac aatcgacgta
     4981 aaaccatatt tttgccgaat cttgtcaata attttatcta aattttcttc gttaatttgc
     5041 ttttgtggat cactaaataa atccagctga agcgaatcgg tgtacacaag cttgctatag
     5101 gttattccaa cgtgccttac ttcttgtcct tcataatact tatcaaacaa gaaaagacaa
     5161 taattctgca attcctttgt attatctgtg ataggtattt tcatctggtg tgaaaatcct
     5221 ggtctcgttt ctaagatcga cgtaccaatg tttaaatgaa cacatcctgt tttacaatta
     5281 tgctgcctga tacgaatggc tacttgttcc gccatttctt ttaccaccag ctcgatttca
     5341 tttcttctag tataatctcg tggtaacact tgtgaattac cataagattt ttcctttgtc
     5401 ggttctggcg ggattgctat atctgttcga tcgattccgt tagcatggaa gtacagctgt
     5461 aatccaatca cacctaatct attcttaatc gtataaggat tccagttagc aaggtctcta
     5521 atgctcaaaa ttcccatttg gttgagcctt tttttcattc gagaaccaat tccccaaaag
     5581 tccgtcattt cgggaatatt ccatactttc tcaggaacgt tttcgtacgt ccattccgca
     5641 ataaatcctt cattatgctt tgcttcattg tctaaggcta atttagccaa aaggggatta
     5701 tcgccgacgc ctaccgtagc aatcaaacct aactcttctt taatacgttc ttggatcatt
     5761 tgagcgagct tcttccttcg ctgacttcgt gtttcttcag tcgtaaaaag attcagtgat
     5821 tttgtcactt ttaaaatcga ttcatcgatc gagtaaatca gtagatcttc atcagccaca
     5881 tatcttctga aaatattatt tacctgcata tttcgcttga tatataggtt catacgtggt
     5941 ggaacaacgt gtagtgtttt aggaaatggt tgtggtaagt cacgtggtct actcacattt
     6001 gtaataccat accgcttttt tgcttcagga gaagaagcta atatcaatcc tgaaccagta
     6061 ttatcagatc gactcataac aacaagttct gttgttaatg gatctaaatt tctttctata
     6121 cattcgacac ttgcgtaaaa gcttttcata tcgattaaaa agtaatcatt tactggctct
     6181 tttgaataat ccatcataaa taacactcca ttcttccaca ttatacaaac atatgttcga
     6241 tgttgtaaag aagaaatagc taaatacata aaaaaagaag cataggtatg ctatcaccct
     6301 gcttcttctt atgatttgta tgatcaattt attctatcac tttactaatt aatttgaaaa
     6361 tctaataaaa actctcactc taaaagagtg agagactgca aattagagta cggacttttt
     6421 tggagttccg ttctttaata ttaacaaatg aataaaatcc ttacaacatt tgataataag
     6481 ttatcagtag acacttacaa aaaattctca tactattgtc acttttctag ataaatggca
     6541 aaaattttca gcattaaatg acaaaaacta atgagaattt gtctttccat ttttctcctc
     6601 catttttgcc aaataacgat aaatggtggt tcttgagacg ttccaccgtt ccgcaatagt
     6661 cttaataggt gtaccgctat ccactaaagt cttcagtaat tccaaatctt gttcagacaa
     6721 tttttcagga cgaccaccaa accgtcctct tgcacgagca gctgctcgtc ctgctgcgga
     6781 tcgttccagg atcaaattac gctcaaattc tgcaaatgcc gcaaacaaat gaaacatgag
     6841 ctgtcctgta gaacttgatt tgtccatcgt aatattttct tgaatactat gaaaactcac
     6901 accacgctca ttcagtcgat tgacgattga aatcaaatct tccatatttc tacctaaacg
     6961 atctaagcgc caaacgacaa gtgtatcgcc agagcgaaca aaatcaatgg ccatttctaa
     7021 tcctggtcga ttactttttg ccccactcat atgatcagta aaaatttttt cacaaccaaa
     7081 tttatttagg ctgtcttctt gtaaatccaa attttgtaaa ccagtagata cccgggcgta
     7141 tcctattttc aaaaaaatca ctccttcatt aattttattg taacataact ttatccatat
     7201 agtttaactg aacatagata ttgttatggg tttatgaaca tgaaatgaat tgtttttttg
     7261 aaaaataaag caaaaaaagg aagtgtccag aaatgggtga ttttatgaac actttcaatt
     7321 agaaaaaaag aatgtcgact agacactctt tttttaaaat cattattttt ctttttgata
     7381 tgttttttgg tctctttttt ttcttttttc ttcagaatct tctagattag aaattgattt
     7441 ttgaattttt tgtgtctcct gtaaagttcg ctgaacttgt aaagctgatt cttctttcgg
     7501 taaattaatt ttacttagaa tatcgctatc ttgataaaaa tttggtgtgt tcacaaattc
     7561 tggcataaaa aatccctact ttctagttaa ggttcctgaa taagtcgcaa tccaattgtt
     7621 tccagctgat ttataacttt gtaaatacaa ttttccatag taccaataac catttctttt
     7681 ttctttccaa ttccgtgatt gaggaggtct agaaggccaa ccaactttat caaaaatcat
     7741 cgttcctgtc actcgtatcg ttttagaagc tgctcgagca agtaatggag aaactattaa
     7801 ataattttca gtaacagtta agtcgttagt tgggttacca atggctattc cgttcctatc
     7861 taaaggagtt aattcttggt tattatctac tacagtagtt ggttcatttg tactatcagc
     7921 aaatgtagga attgtagttc cagttacact aattccaata gttaaaatac ctaaagataa
     7981 aataaactta tttttcattg tctatcacct cgaaatcaaa aataagaaaa cgttttctta
     8041 ataaatctta acagataaat caagttatat actagaaaat tcataaaata tatttttaat
     8101 gtcataatcg tttaaatttt cttgattgac atttatttac aaaaaatatt gccatttaaa
     8161 aagaaaagtg acacatacta tttcctccat cattagtttt tgtctatata aggtataccc
     8221 taactagaca cgaccgcgac attgaaaaat ctggttttct cgtgtatgat tagggtgttt
     8281 tttaaatagt tgcagaaagt aggtaaaaat tttgagagtt gcatacatac gtgtctcatc
     8341 catcgatcaa aatgaacaac ggcagattga agaaatgaaa aaattcggtg cagagagaat
     8401 tttcattgag aaacaatctg gcgcaacgat tactcatcga cctgtttttc aagaagcact
     8461 ggatttcgtc cgagatcaag atatttttat tgtggaagct attgatcgac ttggaagaaa
     8521 ttacgacgag atcatcgctt cagtcaatta cttgaagaag aaaaatgtgc agctgatcat
     8581 cacaagtcta ccgatcatgg ccgaagcaat tggcaatcct ctcctggata aattcattaa
     8641 agatttgatc attcaaattt tggccatgat cgctgaacaa gaacgtaccg aatctaaacg
     8701 acgtcaggca caaggcatta agattgcaaa agcgaacggt gtttataaag gacgtcctaa
     8761 gctatacagt gccaatgcaa aagatcccca acgtcgtctt gtttataaaa acatcgttga
     8821 ggatctaaaa aaaggtgttg cgatcgctaa gattgcaaaa gactacaatg ttacgagaca
     8881 aaccgtatat agaataaaaa aagacagcat ggtcaatcac gaatagttga tcatgctgct
     8941 gtttctattt tatttgctta ctttttgaaa aactgttcta actttcatca ataactcctc
     9001 aactaaacta tcatgcgctt tttccaaaaa aatacactct ctggcttcat aatcaattgt
     9061 cgttagttga tctaataata cttttcctgt tgtcttcatt tcatattctg agtttatatt
     9121 tacatatagc ggatagtttc tagttgtact agaaattgga gcaactgtaa caacgttact
     9181 ataatccgca ataatatcat gacttaaaac aatataaggt cttttacctg tctgttcgtg
     9241 tccagatcga ggtgctgtat tcaataatag aatatcgcct tgtctaggtt ttctcaccat
     9301 ttttcattac ctactgcttc acctaaattt tgaatcgtca catgatttct ttcatcagtg
     9361 taattttcaa ataactgttc aattgttaat tcattttcta tattagccgc ttcgataatt
     9421 agccgcttct gaccgtttac ttcttcttgt attatcttca ctttgtcttc tgtatgaatt
     9481 ccaagatatt ccataatata ctttggcaat ctaaggccat tactatttcc ccattttttt
     9541 ataggtaaca tttccatagt agacacctct ttctgtatat acatagtata acatacataa
     9601 gaaaaaacgt atatacttaa ttatcctttt ctattaaatt agattaacat tccatgaatc
     9661 cataaactat gttaacattt atatgaaaat agtcgaaagg aaatcataat atgtttaaga
     9721 aaacaaaatc tttagaatca ggtaagaaaa aaattgatct aaaaataatc tcttctggat
     9781 actttaaaga tttcatagtt attgatacaa aatggaataa aaaggacata gaaaa
//
LOCUS       DACCKU010000102         9574 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.105, whole genome shotgun sequence.
ACCESSION   DACCKU010000102 DACCKU010000000
VERSION     DACCKU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.105"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            359..640
                     /locus_tag="IVN13_002457"
     CDS             359..640
                     /locus_tag="IVN13_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012907.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            642..1058
                     /locus_tag="IVN13_002458"
     CDS             642..1058
                     /locus_tag="IVN13_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012908.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
     gene            1680..2834
                     /locus_tag="IVN13_002459"
     CDS             1680..2834
                     /locus_tag="IVN13_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HAP7012909.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            2828..3250
                     /locus_tag="IVN13_002460"
     CDS             2828..3250
                     /locus_tag="IVN13_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012910.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3561..3941
                     /locus_tag="IVN13_002461"
     CDS             3561..3941
                     /locus_tag="IVN13_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7012911.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            3923..4837
                     /locus_tag="IVN13_002462"
     CDS             3923..4837
                     /locus_tag="IVN13_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7012912.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            5082..5771
                     /locus_tag="IVN13_002463"
     CDS             5082..5771
                     /locus_tag="IVN13_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012913.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(5892..5978)
                     /locus_tag="IVN13_002464"
     CDS             complement(5892..5978)
                     /locus_tag="IVN13_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP7012914.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            6615..7559
                     /locus_tag="IVN13_002465"
     CDS             6615..7559
                     /locus_tag="IVN13_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP7012915.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            7580..8113
                     /locus_tag="IVN13_002466"
     CDS             7580..8113
                     /locus_tag="IVN13_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7012916.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            8753..8977
                     /locus_tag="IVN13_002467"
     CDS             8753..8977
                     /locus_tag="IVN13_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP7012917.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            8997..9284
                     /locus_tag="IVN13_002468"
     CDS             8997..9284
                     /locus_tag="IVN13_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP7012918.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT         3635 a         1628 c         1670 g         2641 t
ORIGIN      
        1 aataaaaatt gctggtttta ttcttctttt tgcttttgcg agcagagcaa catggaccca
       61 tgttgcgata ttttggtggc gccccaatcc ccgttttttc gttgaatctt actcggactg
      121 cactcgtttt acagctgatt tttctgaatt ttgaaagagc ataaggaaac gtggtatcac
      181 tttcttacga aaggtgctag ataggctgtg cctatcacgt ttctcagaaa aaaatttgct
      241 atcacttttt tgccattgtg gctaggctat gtatagtgtt ttggcgaaaa atagctatac
      301 aaatactata tttgtatggt atacataaat tatacgctga tacgaaagga aaatttccat
      361 ggacatttta gttcgaaata tcaatcccaa aattattgca agaatagata aacgttgtca
      421 ggaactcaca accaaaacgg gcaaaaaatg gagtcgaaat aaatatctaa aagttctaat
      481 cgaaagtgat tttgaccgtc aacttatgaa ttacaaaaaa gatcaattcg atttattact
      541 cgaaaaattt attgccattc aaacagaaaa tacgaacata ctgaatgact atgttcacac
      601 aaacaaccaa ctcattgaaa cacttatcgg aggaatataa catgagaaaa acttctgttc
      661 aaaaacacgt aaccctttat cccagtcacg ttgacgtttt agattccatc atcgaaaaaa
      721 atccagacgt caacaattat tcagaagcca ttcgtttttt atgtatgagt tatgaacctg
      781 ataccaatac aaccacaacc agtaaccaag caaaactcaa tgccatggga aaagaaattt
      841 ctatcatgac agaaatcatg gcagaatttg ctgatttata tctaaaagat actggtgttc
      901 ttatcggtac aaattctaaa gtgtatcaag aagtgaaaga aaaaattgaa aatcaactca
      961 atccttcccg acgaaatcaa cccaaaaatc gtcaaaaaga aaccaatcaa acctttactt
     1021 ctggaaattt tgacccaaca aactttctaa aaagataaaa aaagaaaccg atccctaaaa
     1081 aaattcctcg atttcttttt agggatcagt cttccagcaa gatttgcaag ttggtaccaa
     1141 cttgcgaaaa aaaagaaaaa tccgcttatg caacttcttc tttttgtatt ttgaggcgct
     1201 accccaatcc ccaagtatcg gttatttaat agcgcggaaa tccaaagaat aggacgtacc
     1261 ctgaaacaaa aaaagactgc tctcacgagc agcccaaaat gagattatcc tattcatcaa
     1321 cataaaaata ttacaaagtg aagaactaga gaaagacaaa aaaagcatat caaatcctct
     1381 caaaaataac aagtctttaa aaaatgacag ataaaaatgc acaaagaact aaaaaagaga
     1441 aatcgcgtaa aatcaagcat tagaagctag taactagcta gaccaattaa aaatcacaaa
     1501 gtgagcccct gtatcggttg atacaagggc tacttggcat actcgacatt atgttaacta
     1561 acgctataaa taacgattat gttcccaaaa caatatttta tgaactataa caaaaatttg
     1621 ttatcgcata ttgtatattt taattatagg tgtataccac ttaaatttaa cacaaaggcg
     1681 tgattatttt gaaacccaaa aagaaaattt atctaggtct cctcttttcc ttgatgattg
     1741 gaatttttag tataggaatt acaaatacct atgcttcaga aaattctaat ttcaatatca
     1801 actcccaacc aattgaagaa caagcaatta attttgcgaa aactgatttt gaacactcca
     1861 taacttcaat tcttttaatg gaaaataaca ccgaaactga tattaactat actcttggtt
     1921 cgccatttaa aatgctcgat ggagaaaata cggcaaatag ttctattttc tttccagtat
     1981 tttcagacgc tgataatacg attaaatata tttataccgt caaaaaaaat acaaatggag
     2041 aatattcagg aagtatttca caatttttag caaaagaact tcaaaatcta gtagatagta
     2101 atataacaga tacaacctta ttttttcaag ataatagcat ttattattcc gataatggac
     2161 aaattaaact actttggaaa tcacctttaa gtacagaaaa aaatggtgtt tcttccttta
     2221 cagaaaaaaa tgatcttata tccgtaaata taaaaaataa aattaaaaat ttctctaaaa
     2281 aacaaaattt tcctgcggtt aaacgtgtaa aaagtgtctc ctccggagat aactttatta
     2341 tgattaattg gcgtatagca gaaacccaaa caagttttcc gtggtgtgct gcttacgtag
     2401 aagctgctat cttatgtaac aaaacagatg tacgtgctac ttcagctaga gccattatgc
     2461 attataccta tcctaattta aacaacagtc aaattgaatc aaaaagtctt aatcaagatc
     2521 aaataataaa atatgcaaat tactgtggtg tttatcctaa aaaagtagca cgaatactaa
     2581 acctttcaga ggtccaaaca caactaagaa aaggaaatgc catttatatg ggaactgctg
     2641 gttatggaaa ttatagtaac agccgtcatg cacttgcatt atttgggtgg gtcaatcaag
     2701 gtggttatca aacttactat gtgtggaatc cttggtataa ctacccaact gtaataagtg
     2761 caaatgcagg accagttaca attcctgtac ctggaggtgg ttttacttgg gacaatacca
     2821 ttacaaattg gtaaataaac ccattgcttt agttatgggt ctagtgttag ttcttttctt
     2881 aactagttgt tcaaaaaaac aagaaataca aaagactgat ctgattcctt ttctaggaac
     2941 ttggcaaact aaagatcatt cagaacgtct agtaatcact aatttaaata cttcaaaaaa
     3001 tatagaacaa tataccatct tagtaaaaga gaacaatcat aaaattcaaa tcattgataa
     3061 aaatttaaat ataaatgaac aagttttcat ttacaaagca acaaataatt tactaacact
     3121 gcgctctaat aaaaaacaat tagattacac ttttagtttg gattctccaa aaaagctcgt
     3181 atttattttg acaacgaatg atgggacaga agggacagct aaaccaattg aattttttaa
     3241 agctgattga tgaatagttg tttgatatta caaaacaata atttcaccga aggtgaaaca
     3301 agcgaaactt tacaaaaagt ggctagcatt tgctagcctt ttttgtattg tggagcgtgg
     3361 gttttgaggg ggattcaagg gggagcttgc gcccctttga aaagcggtta tttgcaccaa
     3421 atacgtaggt atttggctag caaatataga gcttgccaag ccctttagta agctttagtg
     3481 agctttactt attgtacctt tttaggtaca atggtattgc gaacgatacg tccgtaggac
     3541 gtactgaaag ggtgggactt atgagcgaac aaaatcaata tttggctagc aatcattcta
     3601 aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc gaatccgaat
     3661 ttgcaaagct gaagcagtca gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag
     3721 ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt gctcaggcaa
     3781 tcgcgcgcga tttagccaaa gctggcggga atatcaatca aattgccaag tggtgcaaca
     3841 cacaccaaca cgacgttgat cctggcgacg cccaacgctt atccgaaaac ctaaaaatca
     3901 tgcaaaagga gctacaaaag atatggcaac agttaaagta agccgaacca cttcttgtag
     3961 ccgagcgatc aattacgccg aaccacgagc aaccgtcaaa acagggataa actgtgacat
     4021 tgattatgcc aaaagcgaaa tgaaacaaat tcgcatgctg tatggaaaag acgatcgtgt
     4081 acaagcacac ttattgatcc agtcttttcg accaggagaa attaccgctg aaaaagccaa
     4141 tcaactaggg gcagagtatg cgaaaaaaat agcccctgac caccaaatcg cgatttacac
     4201 acatacagac aaagaccata ttcataacca cattgtcatt aactccgtca atctggaaac
     4261 tgggaacaaa tttcaagccc atggtcaagc atttttaaac aaatgttatg acctaaatga
     4321 cgagatttgt cttgaacatg acttaacaat cactgaacga gggaagaaac ccgaaaaacg
     4381 aaccatgtct gaaatcaaat taaaagaaaa aaatgaacct gtttggaaag acgaaattcg
     4441 gtttgcgatt gatcaaacaa tgaaaaacaa aaaaactcga acgtataacc aattttgtga
     4501 ttctcttaaa acattcggca ttcattgctt gaatcgtggg aaaaatttta cttacgaatt
     4561 gatccacaaa aagaaaaaag tacgatccaa caaactagga aaggattacg aaaaggagac
     4621 gattttacat gagctggaca gacgagaacg agcctacaat caacaacgaa ccaacgagct
     4681 acgaagagct cgagctagca ttcgcgaaac tgcaaaagaa gtacgaagag aacaacgaac
     4741 tcttacgact gaacaaccgg aaattagacc gactcgaaga aaccatgacc aacacgttga
     4801 aagaggagca gactaccaac aagaattatc gttctaaaac gttacaagac atagaaaatg
     4861 aaataaagaa acaacgtgct tttcttgcac cttataccaa agaaagaata caaactacaa
     4921 ttgattcaca agtcaaaaaa ttccaccatg cgctattaaa ccagcgaaat aaagaacaga
     4981 aaaaagtaat tatgcagcga tcaatggata ttcttgcccc cttatgttgc ttaatcatga
     5041 cactgttagc cttttggctg gtatggggag aataaataga aatggcattc tatttaccct
     5101 atctactcat ttttgccagt attttaggaa ttatttggct tagctataag gcttatcaaa
     5161 caagatattc attaaaaggc tctaaaattc gatttaagag gtttttattg ccaggggtta
     5221 tatttacatt atcaatcgtt ttaagtggtt tattcggcgt tttagaggga aataaacgta
     5281 tttcaagaag tattttatta ggggatatca ctcaaaagta tgaagcagca tgtgataaaa
     5341 agaaaaagga gcaagtgtta acacaaaaaa tggaagagtt taccacttgt tatgaggaaa
     5401 tgaacgatat ttttgtgaat caagaaaaac gtttgacgga taaaaacatg gaaaaactca
     5461 ctcgtcttta tcaaaacttg ccagaaaaac agcaaaaaga ggttcaagac aactatgagc
     5521 gaaccaaaaa agacgtgcaa tattttaaag atacgcagac agaagaagct tgttataatt
     5581 tattcagtga tacaatccct ttttcaacta gtgaacagga acgaaaagaa cggcaacaat
     5641 cagttaccta cgaacgttat aaagcactgc ttcagcaagc aacgaacata caaaatccaa
     5701 cgaaaaaaga aacggcatta aattatctaa aaagtgtcaa ggaatggtta gatcagcaac
     5761 agcaaaattg aatttgatcg gcaatcatgt taaactaaac atgcaaatga tcaaacatta
     5821 gatacacgaa agccccagag aggtcggatt ctctggggct ttttttcagg ttgatgtcgc
     5881 ctttattgat tttacttctt gtttcggtta ttcaaccaat aactgaataa agagacacaa
     5941 acttctataa gaagggggca aatgaacaat tcaaacatta gatacacctc cttccagtcg
     6001 caagactgtc ggataggcga caagttatta tatcttaaaa aattaaactt gtgtggtgtt
     6061 tttaagtatc gcttatttta gggttgttag ggcttgccct gaccgcctgt aaggcgcttt
     6121 aaagctagat aaggattttt atccaagact gtcttaaaac ggcttagata agcattcaga
     6181 accgctcaaa ggcctcttta ccaacatgtt tttagcaaag gagcctttag gcgactgcgc
     6241 agtacaatgt gagagcgtag cgatcacacc tttttggcga ccgtaggaag caaggttttg
     6301 gattggcagt tttgaaaaat gcgcgaacct gtttaaagaa aaaagaagtg gtttttaggc
     6361 gggaaaatta gcccccctct ttttatatct ctttttaaac ctctttttaa acctctttac
     6421 cgtaagctgt gtccttactc taccaatgga tacagaatgt tatgaaggac attttgtctg
     6481 tttatgaatg acattttgtc tgtttatgaa tgacattttg tctgtttatg aaggacattt
     6541 tgtctgtttg aacgtcaaat atgaatgtct tgacatttaa aaaatgtcat tcataattgg
     6601 attgaggtga ttatatggct aatgagattg taaaatacca ccatgaatta aatacaattc
     6661 ctttaagaaa atttacaccg gttgaaatga atttgttttt ttccatcgtg tctagaatgc
     6721 gtgaaaaagg ggataagaca gttcgtttta cgtttgacca actaaaagat ttaagtaact
     6781 acaaagcaac tgcaaatgta cgatttgtag aagatttaaa agcaacttat gaaaaaatct
     6841 tatctttacg ctttggtcgt agaagtgagt ctgggctttc ttacgatatg tttgtaatgt
     6901 ttacagaatt taaaatcaac ggggacgtag aaaatcctta cgtagatata cgaatttacg
     6961 aaaaagcact gcctttgtta aataatttag acgagtgggt acgctattct ttacaacaat
     7021 tcaacgagct acaaagtggc tatgcaaaga ctatgtttcg tctgttaaag caatacagaa
     7081 ccaaaggttt cgcatatttc tccaaagaag actttcttga attattagat attcctaaaa
     7141 gttataagca acctgatata gataaacgag ttataaaacc tatacgtcaa gaactaactg
     7201 caatcttcaa aggactaaca atcaaaaaga aatacggaaa aggacgggga aaacctgtta
     7261 ttggctatca atttacgttc aaacctgaga tcaaaaaagc agacgacttt aacaaacatg
     7321 caccaaccaa agaagaccaa aataaagcat tggctttagc tgataaacaa aaagaatacg
     7381 aagaacccgc tacaacaatt aacgaaaaaa tcaaacagaa gaaacgagaa cttgaactcc
     7441 tccaacaaat caaacaacta gaaaaagaga aaaaagctct tgaaaaacaa acagagtccg
     7501 cactttcgga aaaagagaaa aaaagtttat tatctgattt agataattta ttccagtagc
     7561 aacctaggag gcaaccagta tgcaaccaca agaaaccttt acaataaagg aattgtcaga
     7621 tctttttaag atgagtaggc aagcaatatc gaaacatatc caaaaattag attcttccat
     7681 gatagctaaa aatgagcgtg gttataaggt tgtattgcgt tctggagtat tgcaacttgc
     7741 tcgcaactta gacaatcaaa ggttgctaga taagttgcat aaacatgaac aaaacaacga
     7801 taaaaatgct tatataccaa acaaaaacgt ggttgcaaac aagttgctag atcagttgcg
     7861 tgaagaaaat gaatttttac gagaacaact tgccctcaaa gagcaaaata attctcatat
     7921 acaaaaacta ctcgaccagc aacaacaact caccctgcaa gccaaccaac aaatcgaaaa
     7981 acttcaggag caactgcagt tgacttatac ggaagagtct tcagagaata gttcaacgac
     8041 tctttctgaa gcaacagaaa caatggacga acaaacaaag aattggaaaa aatggtggca
     8101 gctatggaaa tgaagaagga aatacttatg acaaaaaaac acttgctatt cggttctatc
     8161 ctactaggaa tattaagctt tagcccaatg ttattatcag tggccaacaa aggaaataaa
     8221 aaaggggaac taaaactcaa tcctaattac tacaacagtc aaaatgagga agtatttgta
     8281 gaaaaacttg ataaataaga aaagactgct cgtacgagca gtctttctga aagagattat
     8341 cctattatta accttagaaa agaataataa atgaatatga gatatgtaaa aagcataaca
     8401 aacacaaaca aaaataacaa gtatttaaaa aatgaccgat caatattcac aaagaattag
     8461 aaagaaacaa ccgcagaaaa ccaagcctta aaaagagctt ttaacctaga ccaataaaaa
     8521 ttcacaaaat cagcgcctgg atcccttggt acaggcgtta cttggtagta tcgacattat
     8581 gtctactagc tgtttaaata actgatctta taattttatt taaacgatta tgaattcaaa
     8641 actacttttt atgacgttaa tcattgttat ataataaaat gttatataat aaaattacta
     8701 agattattta atataacaag caattttaaa aaaacgaaaa ggaaaaacaa tcatgaaaaa
     8761 gaaagtatta aaacattgtg ttattctagg aatattagga acttgtctag ctggcatcgg
     8821 tacaggaata aaagttgatg cagctactta ctatggaaat ggtctttatt gtaacaaaga
     8881 aaaatgttgg gtagattgga atcaagctaa aggagaaatt ggaaaaatta ttgttaatgg
     8941 ttgggttaat catggtccat gggcacctag aaggtagtgg tagggaggtt attataatgg
     9001 attttactaa agaagaaaaa cttttaaatg caattagtaa agtatacaat gaagcaacta
     9061 tagatgacta tcctgactta aaagaaaagc tctttcttta ttctaaagaa atcagtgagg
     9121 gaaaaagtgt tggtgaagtt agtatgaaat taagtagttt tcttggaaga tatattttaa
     9181 aacataaatt tggattacct aaatctttaa tagaattaca agaaattgtt agtaaggaat
     9241 ctcaagtata tagaggatgg gcttctattg gtatttggag ttaattcttt ggaataaaat
     9301 gaaaatggga ctgaagaatc aattctgatt catcagtccc ataaattata gagccgaaaa
     9361 attatcatat ttatagattt attaatagta aatggcataa gaatctttga acaacgaaaa
     9421 aaatgtagct ctacctaatt attgatttgc aacaatttca ccgaaggtga aacaagcgaa
     9481 actttacaaa aagtggctag catttgctag tcttttttgt attgtggaga gtgggggggg
     9541 agggggattc aagggggagc ttgcgcccct ttga
//
LOCUS       DACCKU010000103         9387 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.106, whole genome shotgun sequence.
ACCESSION   DACCKU010000103 DACCKU010000000
VERSION     DACCKU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 9387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.106"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            2..77
                     /locus_tag="IVN13_002469"
     tRNA            2..77
                     /locus_tag="IVN13_002469"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            79..149
                     /locus_tag="IVN13_002470"
     tRNA            79..149
                     /locus_tag="IVN13_002470"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Gly,seq:tcc)
     gene            163..236
                     /locus_tag="IVN13_002471"
     tRNA            163..236
                     /locus_tag="IVN13_002471"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:197..199,aa:Ile,seq:gat)
     gene            261..348
                     /locus_tag="IVN13_002472"
     tRNA            261..348
                     /locus_tag="IVN13_002472"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:295..297,aa:Ser,seq:gct)
     gene            370..441
                     /locus_tag="IVN13_002473"
     tRNA            370..441
                     /locus_tag="IVN13_002473"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:403..405,aa:Glu,seq:ttc)
     gene            464..553
                     /locus_tag="IVN13_002474"
     tRNA            464..553
                     /locus_tag="IVN13_002474"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:500..502,aa:Ser,seq:tga)
     gene            566..639
                     /locus_tag="IVN13_002475"
     tRNA            566..639
                     /locus_tag="IVN13_002475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:600..602,aa:Met,seq:cat)
     gene            658..730
                     /locus_tag="IVN13_002476"
     tRNA            658..730
                     /locus_tag="IVN13_002476"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:Asp,seq:gtc)
     gene            734..806
                     /locus_tag="IVN13_002477"
     tRNA            734..806
                     /locus_tag="IVN13_002477"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:767..769,aa:Phe,seq:gaa)
     gene            811..881
                     /locus_tag="IVN13_002478"
     tRNA            811..881
                     /locus_tag="IVN13_002478"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:843..845,aa:Gly,seq:tcc)
     gene            894..967
                     /locus_tag="IVN13_002479"
     tRNA            894..967
                     /locus_tag="IVN13_002479"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:928..930,aa:Ile,seq:gat)
     gene            979..1050
                     /locus_tag="IVN13_002480"
     tRNA            979..1050
                     /locus_tag="IVN13_002480"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1012..1014,aa:Glu,seq:ttc)
     gene            1316..2509
                     /locus_tag="IVN13_002481"
     CDS             1316..2509
                     /locus_tag="IVN13_002481"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP7012919.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            2639..4111
                     /locus_tag="IVN13_002482"
     CDS             2639..4111
                     /locus_tag="IVN13_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP7012920.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            4194..4982
                     /locus_tag="IVN13_002483"
     CDS             4194..4982
                     /locus_tag="IVN13_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP7012921.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(5049..5717)
                     /locus_tag="IVN13_002484"
     CDS             complement(5049..5717)
                     /locus_tag="IVN13_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP7012922.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5832..7544
                     /locus_tag="IVN13_002485"
     CDS             5832..7544
                     /locus_tag="IVN13_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012923.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            7547..9319
                     /locus_tag="IVN13_002486"
     CDS             7547..9319
                     /locus_tag="IVN13_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012924.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2767 a         1643 c         2121 g         2856 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgccatgc gggtgtagtt tagtggtaaa accacagcct tccaagctgt
      121 tgtcgcgagt tcgattctcg tcacccgctt ttagtttttt atgggcctat agctcagctg
      181 gttagagcgc acgcctgata agcgtgaggt cgatggttcg agtccattta ggcccattag
      241 tacaaaatct tattggtttt ggggaagtac tcaagtggct gaagaggcgc ccctgctaag
      301 ggtgtaggtc gggaaaccgg cgcgagggtt caaatccctc cttctccgtt tcaatatttt
      361 gttttacctg gcccgttggt caagcggtta agacaccgcc ctttcacggc ggtaacacgg
      421 gttcgaatcc cgtacgggtc attaatttaa tatagtttta catggaggat tacccaagtc
      481 cggctgaagg gaacggtctt gaaaaccgtc aggcgggtaa aaccgtgcaa gggttcgaat
      541 cccttatcct cctttttcat attatcgcgg gatggagcag tctggtagct cgtcgggctc
      601 ataacccgaa ggtcgtaggt tcaaatcctg ctcccgcaat taaaaactaa aaacattggt
      661 tccgtggtgt aggggttaac atgcctgcct gtcacgcagg agatcgcggg ttcgattccc
      721 gtcggaaccg tttggctcgg tagctcagtt ggtagagcaa tggattgaag ctccatgtgt
      781 cggcagttcg attctgtccc gcgccattat gcgggtgtag tttagtggta aaaccacagc
      841 cttccaagct gttgtcgcga gttcgattct cgtcacccgc ttttagtttt tatgggccta
      901 tagctcagct ggttagagcg cacgcctgat aagcgtgagg tcgatggttc gagtccattt
      961 aggcccattt attttattgg cccgttggtc aagcggttaa gacaccgccc tttcacggcg
     1021 gtaacacggg ttcgaatccc gtacgggtca tggaagatac tttttgagta tctttttctt
     1081 tttatctgaa agaatgattt taaaaagctt agcagagtta tctttatgga tgattgtgct
     1141 aagctttttt ggacagttct aatgtaaaat atttttatat tttttaagca aaaaaatgaa
     1201 aaaatacgag cagaaaactg ttttatctgc tatacttggt tcgttgcaag ttcccgaaag
     1261 gatccgcagg acggagatgc cttgtaaccg aatttaacta gggggaatca ttataatggt
     1321 agaaagacac ttatttacat cagaatctgt ttctgaagga catccagata aaattgctga
     1381 ccaaatcagt gatgcaatct tggatgcaat tttaaaacaa gatccaacag cacgagtagc
     1441 gtgtgaaacg tctgtaacaa ctggtcttgt tttagtattt ggagaaatat ctacgacggc
     1501 atatgtagat attcaaaaag tcgtacggga aacaataaaa gaaattggtt atacacgtgc
     1561 aaaattcgga tttgatggag atacagcagc tgtattggtt gcgattgatg aacaatctcc
     1621 tgatattgct caaggagttg atgaagcact tgagatccgg gatgaagata aaaaagacgt
     1681 attagatgag attggtgctg gtgaccaagg tttaatgttt gggtttgctg tagatgaaac
     1741 accagaattg atgcctttac caatcgcttt gagtcatcgt ctggtacgac gcttggcaga
     1801 tttgcgtaaa tcaaatgaat taacgtattt acgtccagat gcaaaatctc aagtaacggt
     1861 tgaatatgat gatcaaggac aaccggaacg cgtagataca atcgttattt caacacagca
     1921 tgatgatgca gtagataatg aaacaattcg tcatgatgtc atcgaaaaag tagtgaagga
     1981 agttattcca gctgaattat tagatgatca aacgaaatat tatatcaatc cgactggccg
     2041 atttgtcatt ggtggtcctc aaggggatgc cggattaaca ggaagaaaaa tcattgttga
     2101 tacgtatggc ggttatgctc gtcatggtgg cggtgctttt tctggtaaag atgcgacaaa
     2161 agttgaccgt tctgccagct atgctgcacg ttatattgct aaaaacattg ttgcagcagg
     2221 gcttgctcgc aaagcagaag tacagctagc ttatgcgatt ggtgttgctc aacctgtttc
     2281 gatctcaatc aatactttcg gtactggaac ggttccggaa gaagaactga ttgctgcagt
     2341 gagagaaaac ttcgatcttc ggccagcagg aatcatcgag atgcttgatt tgcgtcgtcc
     2401 gatttacaaa caaacagctg cttacggtca ttttggtcgt acagatgtag atttgccttg
     2461 ggaacatacg gacaaagtag atgcgttgaa agcaagttta gcaaaataag ttatattcag
     2521 ctggaacgat cgccaagtgg tggttgttcc agcttttttt atggcagtta acctcttgaa
     2581 aaagatagta attatttatc tatttttaga tcctgtcata atgaaaggga ggaataagat
     2641 gaaaaaagaa acaaatgtta ctttggtcac agtgagtatt ttcattgcaa cgttcatgac
     2701 ggctattgaa ggtacaatcg taacaaccgc tatgccaacg attgtcggtt cattacatgg
     2761 aatggaaatc atgaactggg tattttcgat ttatttgctg acaaacgcta tgcttactcc
     2821 gatttacgga aaacttgcgg ataaagtggg acggaaacca atctttattt ttgggacatt
     2881 attgtttatt atcggttcgt tattttgcgg tttatctcaa agcatgattg ggttgattgc
     2941 tgctcgtgcg attcaaggga tgggtgctgg ggcaatgatg ccagtagctt tgacgatcat
     3001 cggcgatctt tattctattg aaaagcgagc aaaagtttta ggtttgaata gttcagcctg
     3061 ggggattgcc agtgtttttg gaccgcttgc gggaggattc atcgttgaaa cgatcagctg
     3121 gcactggatc ttttttatca atgtgccgat cggaattcta ttaattattt tgatttggct
     3181 atatttaaat gaaccaaaaa tcatacatga gtctaaacca atggatattt ttggtagttt
     3241 tagtttgatg gccatgcttg tctcattgct gttagggttc caattattag gagacgaagg
     3301 aatgagtccg cttgtttctg ctttgctgct aggtagtatc gtaatgttag gtatttttgt
     3361 ttttgccgaa aaaagagcta aagatcctgt catcatgatg catttattca aaaatccact
     3421 atttgttgtg gtcaacttgg ctgcagcatt ggctagtggt tttttgatgg gaatcgatgt
     3481 ttatatccct atgtggatgc aaggtgtatt agggctgaat gcagcaattg gtgggctggt
     3541 attagcacca ttatcactag tctggatggt cggttctttc cttgcaagca agtggatgac
     3601 tcaaatgaaa actcattggg tcttgagaat tggactacta atcgcagcta ttgggggcgc
     3661 ttggttgtat cttatgcctt tcagaagtaa ctatttatgg ttttttgcta tatcaacggt
     3721 catcggtata ggcttaggtg tgaccatgac tacctcgact gtcagtgcac aaaatagtgt
     3781 atctcgtgac cagcttggtg tagcaacttc ttttaatacg ttagttcgaa caattggtca
     3841 aacagtcatg gtttctattt ttggtttatt gataaatggg gtaactaaac gagaacttgc
     3901 agcagcaaat ttaacaaatg atgcagatat catgaataaa ctggtgaatc cacaaacggc
     3961 taaatatatt gatgcggata ttttgttacc attaagaaaa attctttatg atgggttaca
     4021 ttccgtttat ctagttggct tgttactcgt gatcattgct ttggtcttaa cgcttttcgt
     4081 tcatgaaaaa agtggtaaaa taaaacagta gtttcggtta agatatgtac gtagccgaat
     4141 cattcaaatt taaagttatt caaaaataac ttcttactaa ggaaggcgaa aatatggcat
     4201 ttattcaagc aaatatttat tcgaatgtgt tagaaatgga agtcaatcta aatgtgatac
     4261 taccgcaaac aacaaaaaag gtgattggca ccagttcaga gcaagagaca acggatatac
     4321 ctgttttata tttacttcat ggaatgggtg gcaatcatag tgtatgggaa agacgtacgt
     4381 cgattgaacg ttatgtagca aactacggat tagctgtcat catgccatct actgatcttg
     4441 gctggtacac agatacagtt tatgacatga agtattggac attcattgca gaagagctac
     4501 ctgagatttg tcatgaatta ttcccgcaat taacaagaaa aagagaaaaa acatatgccg
     4561 ctggattatc gatgggcgga tatggcgcgt taaaactagg ccttgcaaaa tcagaaaatt
     4621 tcgcagcagt aatctctctt tcaggcgctg tttctgtagg aaatcgaatg gatgatcttt
     4681 tgatggttcg taaagcatct ttttgggaag ggatctttgg accattggat caaatcgaag
     4741 gttcaaaaaa tgatccgcaa tacttattga aagaactagt aaaaagcgga aaaccagttc
     4801 caagatttta tatggcttgt ggggaatctg attttcttta tcaggcaaat caggaaatgg
     4861 tatctgctat gcaggaacaa ggcttggcgg tcacctttga acaaggacct ggcgatcata
     4921 actggatctt ctgggaccaa tggattcaac gtgcattggc gtggctaatg gaagaaaaat
     4981 aaaaaaagag tcttaaaaag atagctccta tagagatcac aaacatagtg atctctatag
     5041 gagctttttt actgctcgtt ttctcttctt ttattttgtt ggcataagcc ttcaatatag
     5101 gtttgtgcac tcgttagaaa gagtgctttt tcattcaatt ctgcatggga ctgcatactt
     5161 tctttaaatc cctctaattg attttcaact acataattac gcataaatag aactgtttct
     5221 ttcaaaaatt catttccgtc cataattttt tgtcgaagct gctgttcatg ctgctgatcc
     5281 attaactgtc caatcaataa attgtggaga gaggcaatgg cctgatgact ttgggtgact
     5341 gaaaatcctt cacgaacaag taattggatc atttgctcca aatgttctaa tcgactcgga
     5401 aaagcgggca cagtattgat ttctaattct gccccacaag ggaaacgtgt ataaaggtca
     5461 tagtaatttt ccatatactg cagcaactgt tcattccaag gcaaagttgg atctggaagc
     5521 gctaattctt cttccattgt ttcagccatt ttttgcaaga gctgttgttt gtttttgaaa
     5581 taccaatata aagcaggtgc ttgaacatga attctgtttg cgatgtttcg catggaaagc
     5641 tgcgaaagag cttgtgtttc agccaggata tcaaaggcgg cctgaatgat gattgcttga
     5701 gaaagcttta tttccatttt atcaacctcg tgtattgcat tatcttttta aacatggtaa
     5761 attgttagtc ggtctaattt tacagtgtta aattagtcct gtcaatttaa acaaaggggt
     5821 gtaatcaacg aatgaaatta atcttacgac atgcgaaaaa atataaagta gctgttttta
     5881 tttccttgct gtccgtagca gtcatggttg cagcagcatt atggcaacct aagttactac
     5941 agcaagtatt ggaagctatt attacagaag ataatgatga aatgaaaaca attggtattt
     6001 acctgatcag tctggctttg ctaggattag ccgcaggtgt aacgaacacg attttctctg
     6061 caaaagtagc tcaaggcgta agtgcagata tccgagaaga agcatttcga aagatccaga
     6121 cattttcatt tggaaatatc gaacagttct ctgctggaaa tcttgttgtt cgtctaacaa
     6181 atgatatcac gcaaatccaa aatttagtga tgatttcttt gcaatcactt tttcgaattc
     6241 cattcttatt tattggtgcc ttcattttag ctatgatcac gatgccgcaa ttatggtgga
     6301 tcatcgtagc gcttatcgtt acggtatttc tgattacggc actttcattt acaaggatgg
     6361 gcaagcactt catgatcatt caaaaactga ttgatcgtgt gaatagcatt gcaaaagaaa
     6421 acttactagg gattcgtgta gtgaaatctt ttgtacaaga agaaaaccaa ttgaagtcat
     6481 tcagcaaagt aagtgaggac ttaacaaaac acaacatcat agtcggtaca ttattttctg
     6541 tgatgatccc gtccttcatg ctggctgcca atttggctgt ggttggggcg atcttttttg
     6601 tgagcgatct tgttaaagat gatcctacgt taattggtgg aattgcttca ttcatgaact
     6661 acttgatgca gattatgatg gcaatcatta ttggcggaat gatgatgatg atgacttcaa
     6721 gagcagccgt atcgctgaag cggattgcgg aaattttaga tgctgaacca gatcttactt
     6781 atttagacgt tccagaacaa gagttgacgg gttctgtcac ttttgaccat gtttcttttc
     6841 gttatcctgg tgaggataca gatacgctta aagatatcag cttttctatc aaacctggag
     6901 aaatggttgg tatcgtcggg gcgactggtg ccgggaagtc aacactagca caattgatcc
     6961 ctcgtctatt tgatccgaca gaaggcaaag ttgaagtagg tggtgtagat cttcgtcaag
     7021 taaatgaaaa gagtttaaga caaacggttt cctttgtttt gcaaaaagca attttattct
     7081 caggaacgat tgctcaaaac ttgcgccaag ggaaaaagaa tgcaacagaa aaagagatgg
     7141 aacatgcttc ttctattgct caagcaaaag aattcatcga aaagttatct gatcgttacg
     7201 aagcaccaat agaagaacgt agcagtaact tttctggtgg tcaaaagcaa cgtttatcca
     7261 ttactcgtgg cgttatcggt aatccaaaga tcttgatcct agatgacagt acgagtgcac
     7321 tggatgctcg ctctgaaaag ctggtccgag aagcactcga tcgagatttg aagggaacaa
     7381 cgacgattgt catcgcacaa aaaattgctt ctgttgtcaa agcagaccgc atcttagtat
     7441 tggatgaagg cagattagtc ggcgaaggaa cccatgagga attggtagct ggaaaccgtg
     7501 tctatcagga aatctttgaa actcaaaaag gaacggagga gtaagtatga ctgatctaat
     7561 aaaagcaagt aaatttttct atcactattt gaagcggtac aaactctcct ttttctttat
     7621 ctttatcaca gtgatcattg ctacctatct acaagtaaag gcacctcaat acatcgggga
     7681 agcttttcaa gaattagcta attacgtagg aggcttgatg caaggcgtag acgataaaag
     7741 caaatttgtt tctgtaatat ggaaactctt gctgttttat gtactagcta gtgcagccaa
     7801 ctttatctat agtattttgt tcacacaagt agtagggaag tcaacgaacc gaatgcgtat
     7861 cggtctattc aacaaattgg aaaaattgac gatccgtttc tttgattctc atcaagacgg
     7921 tgaaatttta agtcgtttta ccagtgactt agataatatt caaaatagtt tgaaccaagc
     7981 tttgcttcaa gtcatcacga atgctgtttt acttgtcggg atcttgatca tgatgttccg
     8041 ccaaaatgta gagttagctt gggctacaat tgcttccact ccagtagccg ttttgatagc
     8101 tgtcttgatt atccgcaaag cacggaaata tgtggatctt cagcaagatg aagtggggaa
     8161 actaaatggt tatatggatg aaaaaatcag cggacaacgt gtcatcatca caaatggctt
     8221 acaagaagaa acgattgaag gattcttaga gcataatcaa agtgttcgcg aagcaacctt
     8281 caaaggacaa gcttattctg gtctactatt tccgatgatg caaggaatgt cactggtcaa
     8341 tacagcgata gttatctttt tcggcggctg gttagcatta aatggtgatt tggaacgttc
     8401 ggtggcatta ggcttagttg tgacattcgt ccagtattca caacaatatt accaaccact
     8461 gatgcagatt tcatctggat acagcatgat ccaattagcg attacagggg caagacgatt
     8521 gaatgaaata tttgatgaaa aagatgaagt caaaccagaa aacggtaaag catttgatgg
     8581 tgttgaaaaa ggtcttgcgt tgcagcacgt agattttggt tattctccag aaaaactagt
     8641 cttgaaagat gtctcgatca atgtggacaa aggagaaatg gttgctcttg ttggtccaac
     8701 aggatcgggg aaaacgacga tcatgaatct gctaaaccgt ttttacgatg tcaatgatgg
     8761 ttcagtagtc atcgatggaa tagatatccg agattatgat cttgatgcat tacgtagaca
     8821 tgtaggaatc gttttgcaag aatctgtatt gttctccgga acgatcaaag acaatatcac
     8881 attcggcaaa ccggacgctt cagatgaaga agtcatcaat gcagcaaaac aagcgaatat
     8941 tcatgacttc atcatgacgt tagaaaaagg ttatgataca gagatatccg aagaaaataa
     9001 cttattcagt acaggtcaaa agcagttgat cagtattgct cgaacgatca tcacaaatcc
     9061 atctttattg attttggatg aagcaacaag taatgtagac acggtaacag aagcaaggat
     9121 ccaaaaagca atggatgaag caatcaaagg aagaaccagc tttgtgattg ctcaccggct
     9181 gaaaacaatc ttgaatgcag atagaatcgt tgtcttgcgt gatggggaag tcattgaaga
     9241 aggcagtcat catgagttat tagcagaaaa tggattttat gcggaacttt atcataatca
     9301 atttgtattt gaaagttaaa ttaaaagaac ctcgttataa cggggttctt tttatatatt
     9361 ttatgttgtt tgtttttata aggggca
//
LOCUS       DACCKU010000104         8980 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.107, whole genome shotgun sequence.
ACCESSION   DACCKU010000104 DACCKU010000000
VERSION     DACCKU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.107"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            141..377
                     /locus_tag="IVN13_002487"
     CDS             141..377
                     /locus_tag="IVN13_002487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012925.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASK"
     gene            396..1649
                     /locus_tag="IVN13_002488"
     CDS             396..1649
                     /locus_tag="IVN13_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP7012926.1"
                     /translation="MASDELGDIVTLQIAMLKNSSVRNALKSGVFWDVSEYLKDYKNL
                     SQISEDRIEAASIDGALYGVPIQKDYARAGLVIRKDWLDNLGLDVPTTMDELYEVAKQ
                     FTENDPDGNGVDDTVGFGDRAPSDIRYTSFKLFTSYFGAPNGWAVDDNGKFTPEFDTE
                     EYMKALNFSRDLFENGYLAQDFAVTQKTDQQEQFAQGRTGIYTGMVDIKNLKTMGQGI
                     QDDMELVPVNKISDGEGGGYHVWSEGNGIGGLLAFPKSQVKDEAQLKKLLQFVDDLLE
                     EDVYKLMTGGIEGTHYELNDDGSIEFIDMDLWQQEVQPLSSSRPSEVTFSFKDTDPEK
                     EFSNQLIKENEEFAVMDPSVPLDSPTNNEVGSEIEKIVIDATVQYIMGQLDEDGFKKA
                     VEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            1723..2826
                     /locus_tag="IVN13_002489"
     CDS             1723..2826
                     /locus_tag="IVN13_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP7012927.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            2829..4166
                     /locus_tag="IVN13_002490"
     CDS             2829..4166
                     /locus_tag="IVN13_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7012928.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETGRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKTAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSKQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            4153..5031
                     /locus_tag="IVN13_002491"
     CDS             4153..5031
                     /locus_tag="IVN13_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP7012929.1"
                     /translation="MQLNDFLGAPSSRRTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVAALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            5028..5951
                     /locus_tag="IVN13_002492"
     CDS             5028..5951
                     /locus_tag="IVN13_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP7012930.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKLLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTIKFSEHNNNYETSIKRPEWIHLEKQGVQK"
     gene            5948..6358
                     /locus_tag="IVN13_002493"
     CDS             5948..6358
                     /locus_tag="IVN13_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP7012931.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHPTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            6362..7630
                     /locus_tag="IVN13_002494"
     CDS             6362..7630
                     /locus_tag="IVN13_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP7012932.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     LAKNITGPYELDPGFPMLTASDKPASTLQCTGYGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNAGRILPNADWCDTKCRPSMCRSGRRIYAGTHWLPIVYNRWSHPISFSTRKDLYDG
                     KLVTSILLICIRNHFASRLPTESFKNCIYW"
     gene            7670..8980
                     /locus_tag="IVN13_002495"
     CDS             7670..8980
                     /locus_tag="IVN13_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP7012933.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIRD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
BASE COUNT         2951 a         1513 c         1996 g         2520 t
ORIGIN      
        1 ctttatcggt ggtagtctct tcttttccgc agtcacgtgg acagcaaatt ctatattttg
       61 ccatttggaa tggaagaact catatacaga aaaaacagaa gaaaacaaaa atattattaa
      121 aacagtgaag gagaaaattt atgaaaaaag caacgctcgt aaaattaggt cttagttttg
      181 caggaattct agcacttgcc gcatgcggca gcagttcaag taacagttct tcagagacag
      241 aagatggaaa agctaaaatc acttggttaa atattttgca tcatgcttca ccgcctacag
      301 acacgattgt gaacttgatc gaagaaaaaa ccgatacgga actgacattc aactggattc
      361 ctgatgcttc aaaataagaa agattaacaa ctgcattggc ttctgatgaa ctaggagata
      421 tcgttacact tcaaattgcg atgttgaaaa actcttctgt ccgcaatgcg ttaaaatcag
      481 gcgtattctg ggatgtatca gaatatttaa aagactataa aaatttaagt caaatttctg
      541 aagatagaat cgaagctgcg agtatcgacg gtgccttgta cggcgtaccg atacaaaaag
      601 actacgctcg tgctggctta gtcattcgaa aagactggtt ggataatcta ggactagatg
      661 ttccaactac catggatgag ctttatgaag tagctaaaca atttacagaa aacgatcctg
      721 atggaaatgg tgtggacgac acagttggtt ttggcgatcg tgcgcctagt gatattcgtt
      781 ataccagctt taaactgttc acttcttatt ttggagcacc aaatggctgg gctgtagacg
      841 ataatggcaa attcactcca gaatttgata cagaagagta tatgaaagct ttgaattttt
      901 caagagatct atttgagaat ggctatttgg cacaagactt tgcagttacc caaaagactg
      961 atcagcaaga acaatttgca caaggaagaa ctggaatcta cactggaatg gtggatatca
     1021 agaatctgaa gacgatgggt caaggtattc aagatgatat ggaattagtg cctgtaaaca
     1081 agatctcaga tggtgaaggc ggcggttatc atgtttggtc agaaggaaat ggtattggcg
     1141 gcttgctagc atttcccaaa tctcaagtaa aagacgaagc acaattgaag aaattacttc
     1201 aatttgttga tgatctttta gaagaagatg tgtataaatt gatgactggc ggtatcgaag
     1261 gaacgcatta tgaattgaat gatgatggtt ctatcgaatt tatcgatatg gatctttggc
     1321 aacaagaggt gcaaccgtta tcaagctctc gtcctagtga agtgacgttc tctttcaaag
     1381 atacagaccc tgaaaaagaa ttctctaatc aattgatcaa agaaaatgaa gaatttgctg
     1441 taatggatcc ttctgtgcca ttggattcac cgacgaacaa tgaagtagga tcagaaattg
     1501 aaaaaatcgt cattgatgct acggtgcaat acatcatggg acagcttgac gaagatggat
     1561 tcaaaaaagc tgtagaagac tggaaagcac aaggaggcga tcgaatcaca caagagtacg
     1621 aggaagcata caaagcagcg gcacagtaat atagacgatt gggacaaata cttcttggta
     1681 tttgtcccaa atcctatttt aatttttaaa ggagagcaaa taatggaaaa ctggtcaaag
     1741 aaattagcaa aaagtatcat ggaaagaaca cctcgcttat atgaagaaaa gtggtataaa
     1801 ggaaaatggt cttatgacta tggcgtcgta cttaaagggt tccaattact ttgggaacga
     1861 acccaagaaa aaatttattt tgacttcata aaagacaata tcgactattt cgtgcaagaa
     1921 gatggaacga tccgtggcta cagcgttgaa gaatacaata tcgaccatgt aaatacagga
     1981 aagcttttct ttttattgta caaagaaaca ggcgaagaaa aatataaaaa agcagcagaa
     2041 ctcctttccc agcagttggc aaaccatcca cgcacatccg aaggagcttt ttggcataaa
     2101 gaaatttatc cttatcaaat ttggttggat ggcttatata tgggttctcc tttttatgct
     2161 gaatttatcc agaattttag tgaagaaaaa gattaccaag atgttttgag acagtttgaa
     2221 atcagttata aacatctatt cgatccaaaa acaaatttgc tgattcatgc atgggatgaa
     2281 aagaaggtcc aaccatgggc tgatccaaaa acaggtctat cccatcattt ttggagccga
     2341 tcgattggct ggtatttgat ggctgcagca gatacctatg aattacttca tgaagaaaca
     2401 gattgttctt tgctgaaaga aattctagaa aagacactgg ctgcattact tccatatcaa
     2461 gaagaaaaca gcgggacatg gtatcaagta acaacagaaa caaaacggaa aggaaattat
     2521 ctggaagctt ccggcagtag tatgtttgtt tacgcaatgg ctaaaggaat acgtttgggc
     2581 ctgctgaaca aagaagaatg gcttccacaa gtgaagaaat cacatcaagg attacttgat
     2641 gaatttgttc tggaaacaaa agaaggctgg ctgaacttga ataaaaactg tcaagtagca
     2701 gggttaggcg gagcagatca gcgagatggt tcatatgctt actacatcag tgaaccaatt
     2761 atttgtaatg atcaaaaagg cgttggtgca ttcttgcaag cattgattga agtggaggca
     2821 ttataaatat gtatgatatt ttgacatttg gcgcatcaat tgatgaattg aatactgaag
     2881 caatacaaca agccattgat gctgcagcaa gtgacggagg cgggaccgta gttgttccag
     2941 caggtgaatt tctaacagga gctttatttt taaaaagcaa tgtcgaactt catttaagcg
     3001 caggagcggt tctgaaattc tctgacgatc caaaagatta tcctgttgtt cattcacgtt
     3061 gggaaggagt ccatcgaaaa gtttatgctt cctgtatcta tgctcaaaat gtagagaaca
     3121 tctctgtcac tggtttcggt acattagatg gaaatggaaa aaaatggtgg catactttta
     3181 gaaatgaacc agataatctc gcttatcctc gtcctaaatt gatgagtttc cataactgtc
     3241 atcgaattac tgtgaaagat attaaattga tccagtcacc aagttggacg atcaacccga
     3301 ttctttgcag taatgcaacg tttgataact tgacgatctt gaacccagcg gattcaccaa
     3361 atacagacgg aattgatcca gaatcatgca aaaatgtacg tattagtaat tgccatattg
     3421 acgtgggaga tgactgtatt gcaatcaagg cagggactga agatacgtac gaaaggatcg
     3481 cttgcgaaaa tatcacgatc acaaattgca cgatggtcca tggacatggc ggtgtcgtat
     3541 taggaagtga aatgagtggt agtattcgta atattaccat ttcaaattgt atcttccaag
     3601 aaactggtcg aggaatacga ttgaaatcta gacgaggtcg aggcgggatc gttgaagata
     3661 ttcgagtatc caatatagtc atggataatg tgatgtgtcc atttatactg aatctttatt
     3721 atttctgtgg tccgcgaggg aaagaaccgt acgtatggga aaaaacggct tatccgatag
     3781 atgagcggac accagcattc agaaggatcc atttttcgaa tatcacggca aggaatgtcc
     3841 atgcttctgc aggatttatt tatggattag cagaacagtt tatacaagaa atcacatttg
     3901 acaatattga tgtttcaatg gcccaagatg cagttcccgg tgtccctgct atgatgacag
     3961 ggatagagga aatgagcaag caaggatttt acattggctt tgcaggtaag gtttccttta
     4021 accagatgtc gattgaaaat cacgagggac cagcatttct attagaaaat acgaaagaaa
     4081 tcagtattga gaaattccaa tcaaatcaat cagaaaatat ggatgaactg gttgttcgta
     4141 aaaactttgc ttatgcagtt gaatgatttt ttaggtgctc catctagcag acgtacccct
     4201 agtggaaaac gattataccg agaaaaacaa aacggtttta tgcttgaata ctatcaattg
     4261 tacttaaatg aattggaaac agtaccagcc ctctatgctt atccagaaaa agaaggaccg
     4321 tttccgacgg tcctttacct gcattctcac ggcggagatt tttcagtcgg gaaaagtgaa
     4381 ttgatccatg gtgctcctta cttagcttct ccatcttttg cagaagtact gacaggaatg
     4441 ggatatgctg tttggtcgat tgatgcttgg ggatttgaag aacgcggagg tatttctgaa
     4501 agcgagttgt tcaaacaatt tttattaaca ggaaaaacgt tatggggaat gcgtatttat
     4561 gatgtgatca gcttgatcga ttatttggag acaagggaag acacagatac tcaacggctc
     4621 gcaacgattg gtctttctat gggaggctta ttaagctggt gggtggcagc attggattct
     4681 cgtgtcaaag tttgtattga tctagcggca caggtggaca tcgaaacact tttgctacat
     4741 cgccgcctag atcatcatgg gttttattac tatgtgccaa atctattgac agcttataca
     4801 acgttagcaa tccaagaaaa aatcgctccg cgccctcgtc ttagcttagt tggaaaaaac
     4861 gatacgatgt gcttggacga aggcgtactg aagctgagaa aagggctgga caaaaagtat
     4921 caatcaatgg gtagtccaga aaacttttct tctttttctt taacaggcgg tcatatggaa
     4981 acacaagaaa tgagaaacat ttggaaacaa ttcataaggg agcatttatg aggatgaaag
     5041 acaaactgat aattggaaag aatgctatag ataccccgac ggattttgaa actatccaag
     5101 aaggaatcga ttatttggca ggcctgccgg atagccatcc aaagctcttg atagtaaaag
     5161 aaggaagata tgatgaatat atagaaagca gattatccaa ttttaaactt gtgggagcag
     5221 gaacagtagt tatctccggt aatcgttttg caaaacagga gtatcttgac ggtagccagt
     5281 tagggacttt ccgaagtgct acttttttct tagaaggatc gaatgtcgta ttagaaaatc
     5341 ttcacatcga aaatacagca ggtccaggag aaaaggtagg acaggctgtt gctttattca
     5401 attcaggaga tcgcgtatct ttaaaaaact gtcgactatc tggttatcag gataccttat
     5461 gcacgggtcc gttgcctgat ttgcaaaaag atggtactcc tttttgtaca ccggcttctg
     5521 ccaatcctga gtattgtcgt cagacctatg aatattgctg gattgaagga acggtggatt
     5581 tcatatttgg tggggcagat gccgtttttc atcagtgtca tatccatagc ttagccagta
     5641 aaaatcctgg atacatcaca gcagcatcta cgccgaaaaa tcaaaaaaca gggtatcgtt
     5701 ttgaaagctg taccttgact gcagaacctg atactagaaa cgtctattta ggaagaccgt
     5761 ggcgtccgta tgcaaatgtc ttatttgaag cgtgtaagat aggtgggcat gttcacccaa
     5821 ctggttggga taattgggat gacaagaaaa atgaacagac tatcaaattc tctgaacata
     5881 ataacaatta tgaaacatcg atcaaaagac ctgaatggat acatttagaa aagcaggggg
     5941 ttcagaaatg aaaagaaaat gggaactatt gctaggaatg gtcggtggca gtctatcttt
     6001 gattttcttt ggcgggttag cagttacttt aagtaatatg tctgcgtcag agtttaaaaa
     6061 aagttatcag tcgcttgctg tagatcatcc aactttgtct ttagaaaata catttggatt
     6121 attacaggac atgactggtc tgtttgccgt tgtgttgttt atttcacttg cttttttagc
     6181 agtagcattg tttttgactg ctaaaggaaa atacttaaca actgccactg gtttgtattt
     6241 tataacagga ttcatattgt tgattggcac tcagtttatt gcgtttcctt ttgcattttt
     6301 ttatttcgca gctggggcat tcagtttata tcgagtaaga atgagaaaag gagcctgaat
     6361 catgcagact atcaaagtga aaaatcctat tttaagagga ttcaatccag atcctaatat
     6421 attaagagtg aaagagcggt attacattat tgtctcctct tttgaatggc ttccaggaat
     6481 ccgagtatat gagtcgaaaa atttagtcaa ctggtcacat acaacggata ttttgaccac
     6541 tcaagtatct ttgcaaggaa atcctacaaa tggaagtata tgggcgccac agatcagtta
     6601 tgctgatgga ttgttttatc ttgtttacac agatgtcaaa agtgcaggcc gtccgtttaa
     6661 agatgtccac aattatttga tcactgcatc tgagatcaca ggtccttggt ccgaaccaat
     6721 ttatttaaat agtaacggct ttgatccttc tttgttccat gatcttgaaa cgggaaaaaa
     6781 atggcttgtc aatgaactat gggattatcg tctggacaca ccgaataaat ccagcggtat
     6841 cgtattacaa gaatatgatg cagagaagaa gaagcttgat ggtcccatct ataaaatatt
     6901 tgcaggtaca gaattagcga aaacggaggc accacatttg tattttcgta acgggtatta
     6961 ttatttgatg acggcagaag gcggtacagg aagaggacat tcagttacag tttgcctagc
     7021 aaagaatatt actggtcctt acgagcttga tcctggtttt cctatgctga cagctagtga
     7081 caaacctgct tctacattgc aatgtacagg atatgggagc ttagtacaaa ccccgtcagg
     7141 aagatggtac atgacatatc tttgtaccag accattaaac ggtgcggcta ttttgggaag
     7201 agaaaccgct attcaagaag tgtattggac gtccgacgat tggttaagac ttaaagatag
     7261 ccagacaact ccgatggaag aaataatgat tgagacaaat gaagaggtac aacaagaaaa
     7321 agatcatcag tttatggata catttgaagg agagctaaag aagaactgga atgccggacg
     7381 cattttgcca aatgctgatt ggtgtgatac aaaatgtaga ccaagcatgt gcaggagcgg
     7441 taggcggatt tatgctggca ctcattggtt accaatcgtc tacaacaggt ggagccatcc
     7501 aatcagtttt agtacaagaa aggatttata cgatggcaaa cttgttacca gcattctgct
     7561 tatttgtatc cgcaatcatt ttgctagtag gttacccact gaatcgttca aaaactgtat
     7621 ttattggtga agaattgaaa aaaatcaatg aaagataggg agaaacgtat tgcggacaaa
     7681 aaaagggatt atcaatgctt tgaactttaa aacaaacgac actcaacaag atgatacaat
     7741 aatactgcaa aaagcaatcg atcaaggtgc gaaagaacac ttacctgtat ttattccaaa
     7801 aggcatttat ttagtcggag cccttttttt aaaagataag agtcatctta tctttgaaga
     7861 aggtgcagtc ctcaagggga gaacagatat cgaagcattt ccagaaatcg atacacgagt
     7921 agccggcgta gaaatgaaat ggccagcagc gattttgaat gttctctcgg cgaaagatat
     7981 tcttatagaa ggaaaaggaa taatcgacgg tcaaggagac cattggtggg agctgtattg
     8041 gggaaaagat caaaaaagcg ggacaagagc tgaatacgat caaaagggac ttcgttggat
     8101 tgcggattat gctattaaac gtccacgcgc ttgtttactg tatcatgcag aacatgttgt
     8161 tgttcgtgat ttgatttttc agaaatcagg tttttggaac ttgcaaatca cttattctaa
     8221 cgatgtatta gtagaaaaag taatcatccg caacaatgat ggaccaagta cagatgggat
     8281 tgatattgat tcttcaacaa atgtgcgtgt ctatgaatgt gatttggcct gtggtgatga
     8341 ttgtattgct attaagtctg gcagggatgg caatggtgca agagtcaatc gaaaatcatc
     8401 aagaatcgag gttgctagat gtaaaatccg ttcaggatac ggtgtaacta ttggtagcga
     8461 agttagtgct ggagttagtg acgtatatat tcgcgatatc gatttttttc agtcagactg
     8521 tggtattcga atgaaatctt caaaagaaag agggggagtg atcgaaaata tcagggtaga
     8581 aaatctgaat atgattgatg tacaatttcc tttttcttgg ataatggact ggcataatga
     8641 atacaataga aaaaatagca atgaccttgc tcaaatgcca gaaagttggc aagcagtggc
     8701 tgaagaaata cctgaatcaa agcaaatgac gaaagtcaaa gacatacaca tcaaaaacgt
     8761 acaggcatgc ctttctccag gttactcact tccttcccga gcttttgatt tagtagcatt
     8821 tcctgaaaag cctatagaag atgtctgctt ttctcattgc caaataactg caaaagaatt
     8881 cgggcgaatc gaagcagtac aaaacttatg tttcaaaagc tgtgatttat ctatagaaac
     8941 tggcaataca gctgcaaaca atacttttga caatcgttga
//
LOCUS       DACCKU010000105         8509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.108, whole genome shotgun sequence.
ACCESSION   DACCKU010000105 DACCKU010000000
VERSION     DACCKU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.108"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(151..1104)
                     /gene="menA"
                     /locus_tag="IVN13_002496"
     CDS             complement(151..1104)
                     /gene="menA"
                     /locus_tag="IVN13_002496"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP7012934.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1117..2220)
                     /locus_tag="IVN13_002497"
     CDS             complement(1117..2220)
                     /locus_tag="IVN13_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7012935.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2310..3245)
                     /locus_tag="IVN13_002498"
     CDS             complement(2310..3245)
                     /locus_tag="IVN13_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP7012936.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3547..5481
                     /locus_tag="IVN13_002499"
     CDS             3547..5481
                     /locus_tag="IVN13_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7012937.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5938..6349
                     /locus_tag="IVN13_002500"
                     /pseudo
     CDS             5938..6349
                     /locus_tag="IVN13_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
     gene            6363..6929
                     /locus_tag="IVN13_002501"
     CDS             6363..6929
                     /locus_tag="IVN13_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP7012938.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6952..7938
                     /locus_tag="IVN13_002502"
     CDS             6952..7938
                     /locus_tag="IVN13_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP7012939.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8093..>8509)
                     /gene="scm"
                     /locus_tag="IVN13_002503"
     CDS             complement(8093..>8509)
                     /gene="scm"
                     /locus_tag="IVN13_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP7012940.1"
                     /translation="TDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSST
                     TSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRA
                     LPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2509 a         1644 c         1720 g         2636 t
ORIGIN      
        1 cgactttttc acaacctcct gataaacggt gttcaaaagc ttgcatctgc ggtaataagc
       61 ccaaccaaac gaaaaatata aggagctatt tttatttggc tgttcttatt acttgatgca
      121 gggcagtttt ttcacaacct cttcatgctg ttaaatggct tgcataatga ttgttagtaa
      181 tagccccaat acataactgg cattgaataa gaccatattt ttgatggaat agctgaaact
      241 gataggctgg ggtaacgttt gcttgaattg atccaaatgc ttcttcacaa ttggcaaggt
      301 caagaaggtg accaagatcg gccattcata gataccgctc aacaacccca ttaaaatcac
      361 gaaatagcaa gcatacatca atagctggaa taagactgtt cccgcaggac gtccgatata
      421 aaaaactagt gtatagcgat gattttcaat atcacggtca agatcccgca aattattagc
      481 caacatgata ttggcaatcg taaacaccaa gggtaaggaa gctaatacga ttgctgctat
      541 agcccaaaga tttcctacca aatcaaattg tccagatgaa aagttgactg tcagatcata
      601 gacatctgaa ccgaatgtat tgacataaac gaccatggca aaaattccaa ggcccatagt
      661 caccccactg aaaatttccc ctaaaggcat tcgagataaa ggaatcggtc cgaatgtata
      721 gaatactcca ataaaacaac agataaatcc catcaaaagt aaaagccaac ccgtattgat
      781 cgttagaata acgccgataa caaaaacaaa tgcaatcata gctaaaatca tgtttcgaat
      841 caatacagga gatagatttc tgcggccaat gacattttct tcatacttat atacttcaga
      901 atgagccttt tcataatcca tgtaattatt gatagcggta gtcgctaaat caaaaactag
      961 cattccaata aaaaataaaa aagtgtttag ccaatgtatc tcatgaaaaa aagcgataga
     1021 gaataatacc cctaccgcaa aaggaaatac actagccacc tttgttcgta tttcaactac
     1081 ttctaaaaag atttttagtg tcatggttcg tactccttat tttagttcag aacggtcacc
     1141 catcgtgtaa tgggagtcct ccgataattt gaaggtgtct ttgattggat cggtcacata
     1201 tactttatcg tcttttgtca cgaagatcgc ttccgtcccg ttattcaatt cgctttcgac
     1261 gtattccagt ccttttttca cacccattga aaagactgct gtagacaacc cgtcaccatc
     1321 aattgattta tctgttatga tcgtgacact tgcgatatca ttgtcgaacg gataacccgt
     1381 ttttggatca aacaaatgat ggtatttttt gccatctact tctaaataac gctcgtaaat
     1441 tccagaggta actaatgttt tgttccgttc tttaatagaa cctaaaacag atccgcgagc
     1501 catgttcgga tcttggatgc cgactgtcca gtcttggttt tcgcctcttg ggctgtggcc
     1561 taagacatag acattaccgc ccaagtcaac gatggcagtg gtcactcctt gttttttcaa
     1621 gactttcact acttcgtccg taatgtaacc tttggcgatt gcacctaaat cgatgatcat
     1681 tcctttttct tttagataaa cagttttttc tttgtcattt agttcgatct tatgataatc
     1741 cactaatttc aatgcctgat cgatttcttc ttgggaaggt tttctggcgt catcaaaacc
     1801 gattcgccat aattgtgtga ttgcaccaat cgccatatcg aatccgcctt gagaatcttg
     1861 gctatattcg taggcccgct tgactaacgt gtagacgtca tcagaaactt ttactggttt
     1921 gatcccagct tgctcattca cttcatcgat ctcagaacct ttttgattga tcgtgatttt
     1981 atcaccaagt tcttttactc gatcgaaggc aggtttcaat gctgattctt tgccttcatc
     2041 atacacacgg atacgaacgt aagtccctaa caagaactgt tcatcagaat atggatcttt
     2101 caatatcttg gtagctgctg aactttcagt ggttgtttta tttgctgagt tgctgcagcc
     2161 agttagcacc ataaaagaga tgcccagtgt gattaaggaa aatagtagtt ttttccgcat
     2221 ctttttcctc tcttcatttt gaaaggtgag acgactgcct aatctggcta tcgtcccacc
     2281 ctccaacttt tattagtatg attatgtgat tatttttctg ttacgatatt atctacttcg
     2341 attgtttgag tgtcgccttt ttcagctgca ttcactaatt gttgtgcgta catgatgaat
     2401 gaatgtgagc tgtgtgtagc acctgtaaca acttctacat tagcaggtga gccatcttct
     2461 tcgcccattg ctttgacgaa ttcgtcgttc agagtaggga tgtaagtttt tggttctgta
     2521 ccgctttttg ctttcatatt ttcattatat tctgtatcat cttgttttga tttaccgtct
     2581 ttgtcgatat aatcaaagtt tgattcagtt actttgccgc cagcaactgt catttcgaat
     2641 tgcacacggt agccgttaga gtagttcttt tcttcaagct tgtaagtgcc gtctttcaaa
     2701 tcagcaccat tgtcgatttc gattgtatct gtgttaccag cttgagcagc ttggatcaat
     2761 tgttgtgcgt agttttggaa gctttcagat gagtgagtag ctccagttac gacttccaca
     2821 ccgcttgcgc tttgtgcttt taagaattgg tcattcaact caggaatgta tttttctggg
     2881 tttgtgccag attttgcttc catgtttttg ttgtattctg catcttttgt ttttgattcg
     2941 ccgttttcat tgacgttatc atattttgat tcagtgattt tgccgtcttt tacaaccatt
     3001 tcaaagactg cacggtaacc atttgaataa tttttttctt ctaatttata tgtgccgtct
     3061 tgcaaatctc cgccagctac aacttttgca gatgattctg ttgtgctttt tgcagtcgaa
     3121 gattgtgctg tagatgattc agaagaagag tttgagcttc cgttgtcatc tgcgccgcaa
     3181 gcgcctaaca ctagagctga aaatgctaat acggcaaatc cagttacaaa acgttttttc
     3241 ttcattttaa taccccacca ttccatttct agtccacaat ttttctttgt gattcattat
     3301 acattctaat ctttttagcc tataagtcaa ctatttttga taaataaccc atgtaatatt
     3361 tcaaacgttt tctttcagca aatttcgtga aaaaacgctt tattttaaaa tttgaaaaaa
     3421 atccaaaagg agttagacaa aagaacagaa catctatata atatatggag attgtgtaat
     3481 aagtatcaaa ctaaaagaat tatcatatat tcattatata gatagtctat aaggagcgga
     3541 tgatcaatgg caaagcaaaa tattgtcgtt gtaggagcag gatatgccgg tgtttcagct
     3601 acaaaatact tggcaaagaa actgaaaaaa gaagatgtag caattactct gatcgatcgt
     3661 cattcgtacc atactatgat gaccgagttg catgaagtag ctggcggacg tgtagaacca
     3721 gaagcaatcc aatacgatct gcagcatttg tttgctcgtc aaaagaatgt acaacttgtc
     3781 acagacactg taatcggtat cgataaagaa cgaaaaatcg tcaagaccaa attaggttcc
     3841 tatccttttg atcagctgat ccttggaatg ggcggagagc cgaatgactt tggtacacct
     3901 ggtgtcaaag cacacggatt caccctttgg tcatttgaag attctgtaag gattcgggaa
     3961 catattgaga agacagtagc aaaagctgca ttggaaccag atgatgaact gcgtaaagcc
     4021 atgctgactt tcgttgtctg tggttctgga tttaccggta ttgaaatgat tggggaactg
     4081 atcgactgga aaaatcgcct agccaaagat tataaattag atccaaacga aatcacactg
     4141 atggtcgtag aggcaatgcc gacgatcttg aatatgttgg atcgaaatga tgctgcaaaa
     4201 gctgaacgct atttgcgtaa gcacaacgtg gaattattat tgaatgcacc aatcgtagaa
     4261 gtggctgctg atcatatcaa attgaaagac ggacgcaaca ttccgactca cacactgatt
     4321 tggacagctg gtgtcaaagg aacttctgac gctgctgact ttggattaga atcagcacgc
     4381 ggacagcggt tgatcgccaa tgaatatatg caggcaaaag gctacgaaga taaaaacatc
     4441 tatatcattg gtgatttagt cttttatgaa gaattcccag gaacaccgac accgcagatc
     4501 gttcaagctg ctgaacaaac aggacacaca gccgcagcga atatcgtggc ttcgatcaaa
     4561 ggcggagaaa agcataaatt taaaggaaac tatcaaggat ttatggtttc tattggtgcc
     4621 aaatgggggg tagctaacct atttgataag atccatttaa gcggattttt agctatcgta
     4681 atgaaacata tcgtgaattt gaaatacttc tttgatattc gttctggtta ttatatgttc
     4741 caatatttaa tgcatgagtt tttccatatc aaagatgatc gcaacgtgac tcgaggacat
     4801 tcttctcgtt acggcaatgt cttatggagt gtgccacttc gtattttcta tggtttagtt
     4861 tggttcgttg aagcttccaa aaaaatcatt gggaatggtg aagtgttcaa accaagtact
     4921 tggtttggtg aaggatcatg gttcactgga aacagtgtgt tcccttggac atgggaatac
     4981 aatgtaacag atgttgccag tggtgcttct gctgctggtg atgcaaccac agctgccagc
     5041 ggagcagggg caagtgccgg agaagcagcg actcaagctg cacattttgg tttgagttat
     5101 gcatacggtg aagaaccaat ggctgtgatt gagaagtcac cagactggtt cacaaagatc
     5161 atgcaagtga tcatgccaaa caatgatgta gcaatgttct tccaaaaatt catggtattc
     5221 tttgaaattg ccttggcatt agcattgatt gctggattat tcacatggct atgcagtgcg
     5281 acaacgattg tattggtgat cacgttctgc ttatcaggca tgttttactg ggtaaacatt
     5341 tggttcattt ttgttgcttt cgcattgatg aacggttccg gacgtgcgat tgggttggat
     5401 cgctgggtaa ttccatggct acaacgtaca ctaggtaatt ggtggtacgg tagaccaagt
     5461 tcccagtacg gcaagaaata aatagataaa gaacgttatt tgaatgcaag atttcaatag
     5521 aaaggggctg agacaaaccg tcacagcctc ctttttatgt aaaattaaag gaataagatg
     5581 cccgagataa gcctccatga gcaagacaca ggtgtcacaa cctctgttac aacctcttgt
     5641 tataaacggt gttccagaag tagcttcttc gaaaaataag ccgaaatttc acaaaaactt
     5701 gaaaagtaat tttcgtaaat tccttcttat ttcttgaagt tgcccacttc tgtcacaacc
     5761 tcttgttata aacggtgttc cacctgctgc ttctcggtaa taagcccaaa gaattagaaa
     5821 acatggaaag ctgttttcta ttcttcgttc ttattactca aagcaagaca caggtgtcac
     5881 aacctcttgt ttttatgcta aaattgggtg agttaatctg gaaaaaggag aacaaaaatg
     5941 agccttaaag aatttgctga aaaaagtctg ctcaaacctt gggatgtttt tattattttg
     6001 cttttgactg ttctttcttt tttaccagtc atgatttttg gtcttcagca aaacaccgca
     6061 agaaaaagaa gcggtactgc gtgtggatgg cacagaaatc aaaacattcc ctttgatggc
     6121 tggagaagag agcaacacat atacttacaa agatgaacac ggagattata acttgatcga
     6181 gatagatggc gatcgagtac ggataaaaga agcagattgc gatgaccaga tctgcgtacg
     6241 tcgaggctgg gcaacgaaaa atggcgaaac catcgtctgt ttgccacata aattagtgat
     6301 cgaagtccgt tcaacagatg gaggtgacga agacagtctg atttattaga ccttcacaca
     6361 ccatgagtaa attacaaaaa atgatttata tttcgctact tgttgcacaa ggagtgatca
     6421 ttggtttatt ggaaaatatg atcccttatc ctttttcttt tgcgcctggt gcaaaactag
     6481 gcttagccaa tctaatcacg atcgttgcca tttttaccat gaaaaaacaa gacagcttct
     6541 tactactttg gctacgacta ttcctgacta ctttgctagg cggaacgatt tccacattcc
     6601 tttatagtat gagtggtgct ttgcttagtt attttggaat gttattggtc aagcaattag
     6661 gaccaaaacg tgtcagtatc atcggaatta gtgcggccgg cggtttcatg cataatgtcg
     6721 gacagctttt gatggcttct ttcattgcac aatcatggac agtcatgttg tacttgccag
     6781 tcctttcagt tttagggatt ttatcaggga ttgcaatcgg gatcgcagcg aattatcttt
     6841 tacgacatgt gcagacactt caacgattcc aaaaagatta tgagcaggga accaaacaaa
     6901 aatggagccc aatgtttacc aaaaaataat cgtcacacaa ggagtttttt tatgaagcta
     6961 catccaatgt gggaaaacta tcctgcttta agtaaagaat tgacagaaac actaaaacgg
     7021 atggaagaaa ctgtccgcct gaaaaacaaa cctgtcgaac aagccatcaa agaaacgatc
     7081 catgccggcg gaaagctgtt gcgtcctgcc tatcagctat tgttctcaca atttggacca
     7141 gagcaagatc gagacaaagc catcgctttg gcagcttcta tcgaaatgct ccatacggcc
     7201 acactgatcc atgatgatat cgttgatgaa gcggatctgc gacgtggtca gcctaatatc
     7261 cgtagtcagt ttggtaatgt cgtttctgtc tatgcaggag actatttatt tgtctgctgt
     7321 tttaaactat tgtctgatta ctccacttcc ttgaaaagtc tccaattgaa ttctcgcagt
     7381 atggaaaaag tattgaatgg cgaattaggg cagatggatg atcgctataa ttatgaactt
     7441 tctgtaaaag aatatttgga aaatatttca ggtaagacag ctgaactgtt ccaattaagt
     7501 tgttctgttg gcgcttttga aagtggaaca agcgagcgct tcgcaaaaaa agcaggagat
     7561 atcggacttt ctatcgggat ggcctttcag attatcgatg atattttaga ttatacaaaa
     7621 gagagccagg aaatcggcaa acctgtacta gaagacatgc gtcaaggggt ctattcttta
     7681 ccgttgatct actctttgca aaaaaacaaa ccaaagctac taccttattt agagaagaaa
     7741 gcggcaatga cagaagaaga tgtcgacgcc gtacgcaaaa tcgttgaaca tacaggaggc
     7801 gtggaagaag ctaggaagtt agcggcctct tacacagaaa aggctttgaa ggaaataaag
     7861 aaattacctg ccacctctct tcgaacaaaa gagaatctct tttcccttac ccaactgatt
     7921 ttggatcgca aagactgaat agggaaaggt tcgatacgta aataaatttc tcagaaggaa
     7981 atctacgtgt tttttgacaa attgaattgt agttcttgta tttatgataa aaagcaaaaa
     8041 taaagagcaa cgtccgttag cgacgttgct cttttttatc ttgaatattt ttttaccttt
     8101 tcgatttctt ataactcata aaagtgattc ttaaaccgat tacaagtaat ataacgcctg
     8161 cgagtgttat ccaattattc gattgacttc cagttttagg aagtgctctt tttgatttgt
     8221 ttccattgtc attgcttgtt ttattagtta agttaaagtt cgtcctgtta tccgaacttg
     8281 tttttgagaa ggagacactt gcatgacgtg tatcgcttgt ggtagacgac gtgtctgtac
     8341 tatcgcttga agttgatgaa tctgagctat cacttgtggt agaggatgta tctgtactgt
     8401 cacttgaagc tgacgtgtcc gtgctatcac ttgtagtggt agatgaatct gagctatcac
     8461 ttgtggtaga ggtagtatct gtactgtcac ttgaagctga cgtgtccgt
//
LOCUS       DACCKU010000106         8297 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.109, whole genome shotgun sequence.
ACCESSION   DACCKU010000106 DACCKU010000000
VERSION     DACCKU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.109"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..665)
                     /gene="nth"
                     /locus_tag="IVN13_002504"
     CDS             complement(<1..665)
                     /gene="nth"
                     /locus_tag="IVN13_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP7012941.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKK"
     gene            complement(672..1370)
                     /locus_tag="IVN13_002505"
     CDS             complement(672..1370)
                     /locus_tag="IVN13_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP7012942.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            1638..2822
                     /locus_tag="IVN13_002506"
     CDS             1638..2822
                     /locus_tag="IVN13_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP7012943.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(2828..3271)
                     /locus_tag="IVN13_002507"
     CDS             complement(2828..3271)
                     /locus_tag="IVN13_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7012944.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(3497..3784)
                     /locus_tag="IVN13_002508"
     CDS             complement(3497..3784)
                     /locus_tag="IVN13_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012945.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(3955..5307)
                     /locus_tag="IVN13_002509"
     CDS             complement(3955..5307)
                     /locus_tag="IVN13_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP7012946.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(5580..6026)
                     /locus_tag="IVN13_002510"
     CDS             complement(5580..6026)
                     /locus_tag="IVN13_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP7012947.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(6262..7188)
                     /gene="cysK"
                     /locus_tag="IVN13_002511"
     CDS             complement(6262..7188)
                     /gene="cysK"
                     /locus_tag="IVN13_002511"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP7012948.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            complement(7328..8215)
                     /locus_tag="IVN13_002512"
     CDS             complement(7328..8215)
                     /locus_tag="IVN13_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP7012949.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT         2238 a         1713 c         1388 g         2958 t
ORIGIN      
        1 ttctttttta gcgccttttc ttttagacgc atccgatttt tcccatcttg acatattgat
       61 agcaaaggac atacctcgca cttgggattt cttgccgtgc aatgatatct accaaaaaaa
      121 atcagtgtat gatgtgtctt tacccataat tcttgtggta ctttccgcat cagtgtttct
      181 tctacttcca tgaccgtagc atctagtttg cagatcctca ggcgttttga gacacgttcc
      241 acgtgggtat ctactgcgat tgctggtatc ccgaatgcat cccccaatac gacatttgct
      301 gtctttctgc ctacaccagg aagagacatc aattcttctc ttgacgttgg cacttgccca
      361 tcgaaacgtt caatcagctg ctgtgcacac gctttgatgt tttttgcttt atttcgatac
      421 aaaccaatcg tttttatttt aagaatgatt tcatcgattg aagcttctgc taacgcatcg
      481 ggtgtcggaa aagaggcaaa caaatctggc gttgctttat tcactgatac atctgtcgcc
      541 tgggcactta agatcactgc aatcaataac tcaaatggat tattgtgctt gagttctcca
      601 tgtgcttcgg gaaacattcc atacatcgtt tctagagctt ccattgtttt ttgtttgctt
      661 aacatattcc gctactcctc caaccaattt ttcagtgaga ctttcggcaa ttcttcttgt
      721 tgagctgact gttctacttc tctttgaaga agctgctgtt ttcttcgttt ttggtcttct
      781 tccacttgtt gttttgtacg tagatttttt ctttcccacg atagcaaaat gcggtcaacg
      841 taattaaaac tataagcctg atttaatacg gcttcccgca gtgctagctt caaaatttcc
      901 ggctgataat ggtcttcttc tagccattgt ccgatccgct ggaactcgat cgaagaaagc
      961 gggcgtccaa attcttgttc aaataattga tacactgact ggatttcttt ttcttgtgtt
     1021 ttttcttcgt attttttttc ttgtgttttt aaatactcac ctaatagatc atagatgggg
     1081 taaagattat atcgatctgc ttttttaccg ttgacaatcg tcgtttctat cttgataaat
     1141 ccatttgtta cgagacgatc cagtatttga aaaatctgat cttgtttgat tcccatatcc
     1201 atagcaatca tctgcaaatc agggaaagta tctccttcaa gctgtgccat atgcaactgt
     1261 agaacaaata aaaattcttc agattgtaga ccgatcctat gataattttt aagtaaaaga
     1321 ttagatatga ctgtttgtcc agcgcttaag taattttcta aattcaacat gatttctcct
     1381 ccgctcaatc acagtatacg taaaaaaaac atgggaagca agaacaggtt tcttttctat
     1441 ttacttcact gatcggtcag atttacaatg tcgtctactt gatttttgtt tgttcatgtt
     1501 tttgatgcgt atcttcactt ttcggaataa ataaattaag tagatttcgc tgttccttag
     1561 aatgttgaca aggaaacaaa attactatac actatttttg tttccaagtt aacattattg
     1621 cataaaggag tttttacatg tacagtttca caaataaaca ttctaaagtt tttggatacg
     1681 tctctgtctt attcactgga ttaggattta ctatcgtcag tcctgtactt ccgtttttaa
     1741 cgctgcctta ttctcactcc atacatcagc aagctttcta tatcactttg ctcatgtctg
     1801 tttatgctct agctgccttc ttatctgccc ccattctcgg tagcttaagt gatcattttg
     1861 ggcgtcatcc gatccttatt atcagtttat taggatcaag tatcggatat ttgctttttg
     1921 gtttaggaaa ttcgatatgg atgttgttcc ttggtcgtat cattgaagga ttgacggcag
     1981 gagaaatctc caccttatat gcttactttg ccgacatcac ggaacctaat gaaagaacca
     2041 aagtttttgg atggatgggt gctttagtcg gtataggaac aacgctgggt cctattatag
     2101 ggggtctatt agctgagttg gggaattctg ttcctatatt tattggagca ctctttactt
     2161 tcttaaatgc cgtttatggc tacacattta tgctagaaag ccttccaata aaaaaacgtt
     2221 cagtagattt aagtttctcc catgtaagac cttttcacca attacaacaa ttattcaaga
     2281 tttcatccgt tattccttta ttgacagcag gttttgctgt ttggttggca gctggctctc
     2341 ttcaatctat cttttctcaa ttttcgattg acgcctttca gtggaaggct gggttggttg
     2401 gtttaagctt ctctttgatt ggaatcttgg atactgtctc gcaattgttc attatgcctc
     2461 gattgctcaa aaaattttca gaacagcaga tcacaagaat agggatgttc agtgaaatcc
     2521 tcgcctatct attcatcaca ttatctggaa ttcttctact gccgattttg tttttatttg
     2581 ggattatctg ttatggtttc ggtgattcta tttttactcc tgtattcaat ggccaactat
     2641 ccaattctgt ctcagataac cagcaaggtc ttgtaatggg cggtacccaa agtatacaat
     2701 ctctatcgcg cgtcatcggc ccgctaatcg caggacagct ttatgctgca gcaccatttc
     2761 ttccgacggt attcggattt ttgcttatga tttttgccta ttatcactac aataaaaaat
     2821 agtagcttca tttgctactt cgtataaatt ctctgtatcg accgataaaa cgaaatactg
     2881 tgtcaaattc ttcttttgac atattttcaa atacagtagc atctcgctgt acccattttt
     2941 gatggagttg ctcgtgtcta ttccttattt tttgtccgat tggcgtcaat tgaaaataga
     3001 tttctttttt attctcttcg ctttgataag tgtttatgat ccctctagct atcagttttt
     3061 ttgttagctt actgatggct cctctagtca tgtaactagc attcgctaat tttgtcacgt
     3121 taggatcagg gattttcccg atatgttcaa tcacttctag ttcatttaga gtaaatcctg
     3181 caaattcttt ttccatttct ggtttattac gatgaataat ctcattagct aaatccagat
     3241 attcttctat tagcagttgt cttttatcca tttctacacc ttctttttct ttcattatat
     3301 tgtttttttg tttcctattc aacaaagata aaaatatttt gtaggaaaac ccaatctgaa
     3361 accaagaaaa aaaggggttg acaaagcttg tcataacccc tttttctgat taaaagatag
     3421 taaagagaca gtcatcggta attgtgatga ttttttgtca tcacttaagg atgagcggcc
     3481 ttttcaaaaa ctgtctttaa agatcatcat cagtttctag cacatatggc tgaccttcat
     3541 atgtgtactc tgtattcaat gtgccgtcat tttcatctga gtagatgcgt ctactgaaag
     3601 gaaaacgatg agaaacatta tctttggcgt catttgctgg catcgtgtaa tattcctctt
     3661 gcgtttcatt caactgagag agtacttgat ctgccaactc atcaatattg atcgttaatt
     3721 tcttcgaagc acttttataa tcttctaaaa atgcatcttg ttggacaaaa tattctttat
     3781 tcatcttctt tctcaccttt cttttcaaaa tcaaataaag ctgcttgttc ttttatgtaa
     3841 cagctttatc tttgctgctt attgtgacaa aaaattcgaa gaaagacaac taattgtact
     3901 tttcacaaaa aaagacagag ggtcgtgaca aaattgtgtc acaacccttt ttctttattg
     3961 tgcagtttcg atttcgccta gcgctgcttg tgcaagtagg ttcaagtaat tccatgggcg
     4021 gtcaaaatgt ggttggaaga agaaatctga aagtgcgaga tcttctacag tcattttatt
     4081 ttgaacagct aaagacatgg tatttgctga ttgtgtaatg tcgtatttgg acatgaactg
     4141 agcacctacg atacgattcg ttcctttttc atagactagc tccatcatta ctttttcagt
     4201 agttggcatg aactctggac gataattatc ttcaaataca gtcgcttgga cttctagatc
     4261 gttcaaagtt gcgctttctt tggtcacacc tgtcgaaccg atcgtccagc caaataggta
     4321 aagtcctgaa gttccttgag taccacgata cgccatttta tgatctgtca agttatttcc
     4381 cacaagcaaa ccttgacgaa cggcatttgt tgccaaagga atatagtttt gcgtgccact
     4441 tggattgtag ttgactactg cagagtctcc agctgcaaaa atgtctggat tacttgtctg
     4501 catgtattca ttcactttga ttgccccgtt tggcagtgta tctacttttc cttcgatcag
     4561 ttttgtattt ggacggaagc ccacacataa aatgaccata tctgtatcaa atgtatctgt
     4621 tggtgtagct acttgtttca cttttccttc ttcatctgtg ataaattcag taacattttc
     4681 gcctagcgca agagtaacac cgcggtctgt caattctttt tctaagatat ctgtgaatgg
     4741 tttatctaga tatttattca agatgcgatc taatccatca atcaatgtca cttgttttcc
     4801 agattcgaca aaagcttcta ctaattcaat accgatatat ccaccaccaa caattactac
     4861 acgttttgct tctttcgctt gtgcaataat ttcatttgct tgattgtagt ttttgcatag
     4921 gagtacattt ttcgcatcga ttccactaat cggtgggata ataggccatg aaccagttgt
     4981 catcactaat ttatcataag aaaccgtttc tgtttctcct gttttcagat cttttgccgt
     5041 tactgtttta ttttctgtat cgacgtctgt tacatcgtgt tccattttaa ctgttgctcc
     5101 taatgaagat aattcttcag gatttgagta gaacaagcct gctgggtcct ttactactcc
     5161 gccgacataa agtgcgatac cgcaagataa gaaagaaata ttgtcatttc tttcatatac
     5221 tgtcacttct gcttctggat gattttttaa aatacttttt acagctgcag tacctgcatg
     5281 agtacagcca attactacta ctttcattta tggtccctcc gattaacatt ttgtccttcg
     5341 acaaaactac tataacagaa ataaaacgga tacaacagtt taatgcttgt gaattcacaa
     5401 aggtttgttt gtagtttcat tccttttcaa gcataagaac ccaatatcta caagattctt
     5461 attttaataa tattcacttt tatatctaag attgtgaata aaagacaaaa aaataaaaaa
     5521 gctagcgttt tttttacaca gctagctttt tttaggtata acacttgtag aaactgtttt
     5581 tacatttgcg ccttttttgc tgactcttta caatccggac agagtccata caattccaac
     5641 cgatgttcat taatctcaaa atcagtcagt ctgcttgcag ccatttctat atcttcaagc
     5701 cctgggtaat gaaaatcaac aatcttaccg cagttttcac aaattacatg ataatgacgc
     5761 tttgaactga aatcaaatcg gctagaagca tcgccgtact tcatttcttg aacaaatcca
     5821 atcgaagtaa ataagcgaag gttattgtaa acagtcgcaa cactcatatt tggaaaccga
     5881 tgttcaagtg ctttataaat ctcatcagcc gttggatggg aatgatgttc aatcaaatat
     5941 tctaaaaccg cgtaccgttg aggtgtgatt cgaatgttct ctttttttaa cttttcgata
     6001 acctcttcga ctaacttatt ttccatgtaa acaccccacg ttgttattct cgtcttatca
     6061 tactattttt acattttaaa atccacttaa catgaaaaga aaattcaatt ttattgtatt
     6121 ttaatgaaaa aagacgtaaa acagtcgcgt tcactattct acgtctctat tcaaataatt
     6181 tttctcatca taaatttttg tgtgttcttt tcatctttaa aagtttttta cataaaccta
     6241 tctggagaaa aaccaagtgc cttatatctc ttggtaaagg gctgtagata aataacgttc
     6301 accattgtcg ggaaccacag ctaatacctt cttgcctttt cctaattctt ttgcgacttt
     6361 tagcgcggca gcaattgcag cgccagatga gatgcctact aaaatccctt cttttcgccc
     6421 aacttctcta gctgtttcca tcgcttcgtc tccactaata gaaaggactt tatcatacac
     6481 agaagtatcc agtgttttag gaacaaaacc tgtaccgatt ccttggattt tgtgaggtcc
     6541 cgcttctttc ccttctaaaa tagcagattc tgctggttct accccgatta attcgatttt
     6601 cggatacaca cgttttaatt ctcggccagc gccagtgatc gttcctcctg taccgatacc
     6661 tgcaacaaac gcgtccaagc catttactcc aaatgcttga tgtatctcag gaccagtcgt
     6721 cttctcatgc acaagaggat ttgcttcatt ttcaaattgt aatggtaaaa agtagccgtt
     6781 ttcttttgcc aaacgttctg cttctctgat tgatccagaa ataccatccg caccaggagt
     6841 caaaatcaac tctgcgccgt accctttcat caataaacgt ctttcgatac tcatcgtctc
     6901 aggcatcacg atgattactt tatatccttt tgcaactcct accatcgaca atccgattcc
     6961 tgtatttcca gaagtaggtt caatgatagt atcacctggt ttcagtagac catcgtgttc
     7021 tgctttttct atcatactta aagcaatacg atcttttaca cttcctcctg gattaaaaaa
     7081 ctcaagcttt acaaaaacat ctgctgaatc ttctggtacg attttattta atttgacgat
     7141 tggcgttttg ccaatcaatt cggtgactga gttatagata gctgtcatct tcaattcctc
     7201 cttgtctttg ctcttcttac ttatcataat acaatctgtt catttatggc gagtgattcg
     7261 tctttgacta gcaaaaaaaa gacaaagata taccatatat ggtaatttct ttgccttttt
     7321 atctaaatca aacgtttagt tcttgaccaa ctaataaaga ataactggac atagacatgc
     7381 catttttcgc cattagatca tcaacagaca tgccaaattt ttgtgcaata tcccatagcg
     7441 tatcacctga cacgactgta tatgtctgac cattatttgt atttacatct gttgttgaag
     7501 atgatgacgt tgaagtcgtc gtagtagtcg ttgttgttgt acttgtggtt gttgtagccg
     7561 aacctgttga tggcgtatca aaacgggtta aattataagt attaatcaat gagttcaatt
     7621 tggccccata atttggatcg gtagcataac gacctgtcaa gtacgctgtt gcatcataaa
     7681 aacttgttgt attgcttttc cacacgccag cataatgata atcgcccgtg gaaaaactgg
     7741 ttgaacgaag aacattcgca tggtcttgga aggattccca ataagaagaa tattttcgga
     7801 atggctcatt catcgttacc cattgtccgt ttacatattc tttggttgcc atataaacga
     7861 cctgtccgtt atagcttcct ttcacaccga atagattata atttggcgca ctagctaatc
     7921 cggaagtgcc atatgcactt tccaacaacg cttgagcgat catgactgaa gcatacaagt
     7981 cattacttgc agcaacttct gacgcagacc agccgatttg atcgataaac gcttgaggat
     8041 cagcgactgt ttgagatcct tgttcatctg catgaacatg ttgcggtacc gctgtaacca
     8101 tcatcggccc aactaaaaga ctcgttccaa ttaaaggagc aatcgctttt ttattgatca
     8161 ccatttttct tttaggtcta tggtgccggg cactgcgggt cttcagctct tgcattcaaa
     8221 ttcctcctca aaataaaaaa acttaataaa aagttctctt cttaaaacaa acttaattaa
     8281 ttaaattata cttttaa
//
LOCUS       DACCKU010000107         8198 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.110, whole genome shotgun sequence.
ACCESSION   DACCKU010000107 DACCKU010000000
VERSION     DACCKU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.110"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..597
                     /locus_tag="IVN13_002513"
     CDS             <1..597
                     /locus_tag="IVN13_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP7012950.1"
                     /translation="VAISFDDGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIV
                     KQTAEAGHEIGNHTYDHQNLATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVT
                     NVGATIIQRPIIEWSVDSEDWKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQ
                     VIDYLKEQGYRITTVGDLLGHPTAVENYYGRNDHRPVQ"
     gene            complement(789..860)
                     /locus_tag="IVN13_002514"
     tRNA            complement(789..860)
                     /locus_tag="IVN13_002514"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(825..827),aa:Arg,seq:ccg)
     gene            920..2254
                     /gene="rpoN"
                     /locus_tag="IVN13_002515"
     CDS             920..2254
                     /gene="rpoN"
                     /locus_tag="IVN13_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP7012951.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            2457..3494
                     /locus_tag="IVN13_002516"
     CDS             2457..3494
                     /locus_tag="IVN13_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP7012952.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            3535..4536
                     /gene="gap"
                     /locus_tag="IVN13_002517"
     CDS             3535..4536
                     /gene="gap"
                     /locus_tag="IVN13_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP7012953.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            4646..5836
                     /locus_tag="IVN13_002518"
     CDS             4646..5836
                     /locus_tag="IVN13_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP7012954.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            5940..6695
                     /locus_tag="IVN13_002519"
     CDS             5940..6695
                     /locus_tag="IVN13_002519"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7012955.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            6811..8109
                     /gene="eno"
                     /locus_tag="IVN13_002520"
     CDS             6811..8109
                     /gene="eno"
                     /locus_tag="IVN13_002520"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP7012956.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         2816 a         1535 c         1732 g         2115 t
ORIGIN      
        1 gtcgcaatca gctttgatga tggaccagga gcgaccacaa caccgcagct tctgcgaatc
       61 ttaaaagaaa aaaacgtgca tgccactttt ttcgtcttag gagaaaatac ggcgcagcat
      121 cctgaaatcg tgaagcagac agctgaagca gggcacgaga ttggcaatca cacatacgat
      181 catcagaatc tggccactct ttctgcccag tcgatgacgg aggaagtgac gaaagcagac
      241 actgagatca aaaaagtcac tggcaaaacc cccacttttg ttcgtccgcc ctatggttct
      301 gtcacgaatg taggagcaac gatcatccag cgaccgatca tcgaatggtc tgttgattca
      361 gaagattgga agacaagaaa tcctgatttg attttgcaga agatccaagc aacagtatac
      421 gatggggcaa ttattttatt ccatgatatc tatcctgaaa cgattcgtgc cgttcctcaa
      481 gtgatcgatt atctgaaaga gcaagggtat cgcatcacga cagttgggga tttattaggg
      541 catccaacgg ctgtcgagaa ctattatggc agaaacgatc atcggccagt tcagtaagtg
      601 agacaagtat ctttaataaa taacacattt taataagtag ttgaacacat aactacctta
      661 cttgcaaagg cttaacaata tctatacagg tacaacaaaa aactgtccac caattttctt
      721 tggcggacag ttttcactta aaaataaaga agcaactatt cgtaaattat gctaaaatag
      781 cagtagaacg cgtcctgagg gaatcgaacc cccgtctcaa gaaccggaat cttacgtgat
      841 atccactaca ctaaggacgc aagcactggg ataattgtaa ctaaacagat aaaaaattac
      901 aaggggattg gacaaaaaaa tgaaatttga gcaaaatttt tcacaaaaac agcagcagac
      961 acagaaacta gccatgacgc aacagttgca acagtccatc caagttttac aatataacag
     1021 tgaggagctg ttggcattcg ttgagaatca agcaatggaa aatccattgg tggaagttgt
     1081 tgagccggaa tggcagccag attacataaa agcttcttca tcttcttatg aaggagaaga
     1141 gacaaattac ctaaatcaga ttcctgatac aaaagggtct ttatttgatt cattgatcga
     1201 acaagtccat ttgaattacc gagatacttt tttgaggaaa atcgttcttt atcttgtaga
     1261 atacatcgat ttgaacggat ttttgacgat tagtttagaa gaagccggaa aagagattgg
     1321 agctacgcct atccaaatgc tcgatgcgtt gactctgatt cagcaattag aaccagctgg
     1381 agtaggtgca cgtagtttgc aagaatgtct catgcttcaa acagaacgag atgattatgc
     1441 gccggaatta gcttatatcg tgttggaaga atgttttgag gaactggtgg agcgaaaatg
     1501 gaaagaaatc gcacaacgtt tttctgttga tctacatgct gttcagcaga tattcgacta
     1561 tttacaaacg ttgagtccaa gccctggccg gattttcgac cgtagcagcg aattgtacat
     1621 tattcctgat gtccgggtat tagtggacga cgataaaaat gtacaagtta tctcaaaccg
     1681 aaaaaatcag ccaaatatac gctttcaaga gagttacttc aaacaaatgt cacaacaagc
     1741 agataaagaa actgaaaatt atttaaaaga acggaaacag gaattcgaat ggttgaagaa
     1801 aacgatccta caacgtgggg atacaatttt gcgggttgct caagtcattg tttcccggca
     1861 aaaagctttc tttatcgaca aggaacgtcc aatcaagcct cttacgttaa aggaagttgc
     1921 aactgagata gatgtgcatg aatccacggt cagtcgtgca gtcaacggga aatatttgga
     1981 aacagacttc ggtgtatttg aattaaaaaa atttttcact acacgaattc ctacgaatag
     2041 tacagaacag acagaagacc tttcagcaga tacagcgaag aaaaagctgc aggaattagt
     2101 cgatcaagaa gacaaaaaca agccgttgtc tgaccagaaa ttggttgaat tgctgaaaaa
     2161 agatgagata gcgatctcaa gacgaacagt agcaaaatat cgtgatttat taggaatccc
     2221 gagttcatca aaaagaaaac gttatgataa ctaatcagtc aaaagactag aaaaagtatt
     2281 tcactttttc tagtcttttt agttgaaaaa cgagagcaaa actgttagac ttacagtcgt
     2341 gaggttgaga tttcccttta ttgatgtgaa attcgaatag ttaatttctt tttttatcgt
     2401 atatgggtcg ttttaagtct acattggacg taaagtgtcc aactaaggag atcgctatgc
     2461 tggatgaaat aaaaatgatt gaatctgttg caccagatat tatcgaagtg ttgcaagaac
     2521 gctataaaat tttacgaaat atctattgga tgcagccgat tggacgtcga agtttatcgg
     2581 aaagtatggg gatcacagaa cgagtattgc gaacagaaac agatcttttg aaaaatctga
     2641 atttgatcga cacctcaaaa agcggtatga cgctgacaac taagggcgaa gaagtttatc
     2701 aaagccttga aaacttcatg gatcagcttt taggtacgca tcaaaccgaa caacagttgg
     2761 cagaatattt tggtaaccaa cgctgtatcg ttgtttcagg aaacagcgaa gagcagacca
     2821 aagtggctga tgcattcggc gaagcgctga gcgaagccct agaccgacta cttccagaag
     2881 gcgagaatat catcgctgtg atgggaggaa ctactatggc tgtggtagcc gaacaattgt
     2941 ctaatctgga aaataagaaa cggcataacc tgtttgtccc tgcaagaggc gggattggtg
     3001 aagcgataac tgtacaggca aattctgtca gtgcaagaat ggctgctaaa gcaaacggta
     3061 accaccgtgc actttatgtt ccggaacaat taagtctggc aacttacaat tctttactca
     3121 atgagccatc tatccaagaa gtcttaaact tgattagtga agcgaattgt gttattcata
     3181 gtattgggcg tgcattacac atggcagcac gtcgtaagat gactgaaaaa gaattagtca
     3241 tgctgaaaca agcaaatgct gttgcggaat cttttggcta ctttttcaat gaaaaaggtg
     3301 aagtcgttta taaggtccct agaatcggca tagaattgca agatttggaa aaagtcccga
     3361 tcatcatggc aatcgcgggc ggaaaatcaa aagctaaagc aattcgagct tatatgaaaa
     3421 atgcaccaaa acaaacgtgg ctcatcactg atgaagctgc tgcaaatgag attttaaaag
     3481 gggtaaccct ttaaaataaa tatttttttg attttcataa ggaggaaatc ttaaatgaca
     3541 gttaaagtag gtattaatgg ttttggacgt attggacgtc tagcattccg tcgtatccaa
     3601 gatgtagatg gaatcgaagt agtagcaatc aacgacttga cagatgctaa aatgttggca
     3661 cacttgttaa aatacgacac aactcaagga cgtttcaacg gaacagttga agttcatgaa
     3721 ggttcattca acgtaaacgg aaaagaagtt aaagtattag ctaaccgcaa cccagaagaa
     3781 ttaccatggg gcgaactagg cgtagatatc gttctagaat gtactggttt cttcacttct
     3841 aaatcagcag ctgaaaaaca tttaacagct ggtgctaaac gcgttgttat ctctgctcct
     3901 ggcggaaacg atgttccaac aatcgtttat aacacaaacc acgaaacatt aactggtaaa
     3961 gaaacagtta tctcaggtgc ttcatgtact actaactgtt tagctcctat ggctaaaaca
     4021 ttgaacgaca aatttggtgt tgttgaagga ttaatgacaa ctatccacgc ttacacaggt
     4081 gaccaaatga ctctagacgg acctcatcct aaaggtgact tccgccgtgc acgcgctgct
     4141 gctgcaaaca tcgttcctaa ctcaacaggt gctgctaaag ctatcggttt agtaattcct
     4201 gaattgaacg gtaaattaga cggagctgct caacgtgttc ctgtaccaac aggttcatta
     4261 acagaattag taacagttct tgaaaaagaa gtaactgttg acgaaatcaa tgcagcaatg
     4321 aaagaagctt caaacgaatc ttacggatac aacacagacg aaatcgtttc ttcagatatc
     4381 gttggtatga cttacggttc attatttgat gctacacaaa ctaaagtaat gacagttggc
     4441 gacaaacaat tagttaaaac tgttgcttgg tacgacaacg aaatgtcata cactgcacaa
     4501 ttagtacgta ctttagaata cttcgctaac ttataagata gacaaacgta caagtgtaaa
     4561 ttatagacat gtacatttaa aagcggggaa gcaatcgcgc ttcttccgct ttttttgata
     4621 ataagtttat tcaggaggaa ataaaatggc taagaaaaca gtgaaagata tcgatttaaa
     4681 agacaaaaaa gttcttgtcc gtgttgactt caacgttcct ttgaaagacg gcgtgatcac
     4741 tgacgacact cgtatcaaag cagcattgcc aacaatcaac tacgttctag aacaaggcgg
     4801 aaaagcaatc cttttctctc accttggacg tgtaaaaaca gaagaagata aagaaggaaa
     4861 atctttagct ccagttgcaa aacgtctagg cgaattatta ggtaaagaag taacattcgt
     4921 acctgaaact cgcggagaac aattagaaga agcaatccgc aacatgaatg acggcgacgt
     4981 tgtcgtattc gaaaatacac gatttgaaga tattgacggt aaaaaagaaa gcaaaaatga
     5041 tacagaacta ggtaaatact gggcttcatt aggcgatgta tttgttaacg atgctttcgg
     5101 tacagctcac cgtgcacatg cttctaacgt aggtatcgct tcaactggta tcccaacagt
     5161 tgctggattc ttgatggaaa aagaaatcaa gttcatcggt gaagcagtag aagaaccaaa
     5221 acgaccaatg attgcaatcc ttggtggcgc aaaagtttct gataaaatcg gcgtaatcga
     5281 aaacttgatt cctaaagcag ataaaatctt gatcggtggc ggaatgactt acacattcta
     5341 cgaagcaaaa ggaatcaaga tcggtaactc tctagtagaa gctgataaag tggaactagc
     5401 aaaagaatta atcgaaaaag caggcgacaa acttgtcttg ccaatcgaca atgtctgtgc
     5461 accagaattt tcaaatgatg ttgaaacaca agtaatcgaa ggagacattc ctgatggttt
     5521 gatggcttta gacatcggcc cagcatcagt aaaattattt gctgatacat tacaaggtgc
     5581 aaaaacagtt gtttggaacg gacctatggg tgtatttgaa atgagtaact ttgctaaagg
     5641 tacaatcggt gtttgtgaag caatcgctaa cttagaaggc gcaacaacaa tcatcggtgg
     5701 cggagactca gctgcagcag cagaacaatt aggatttgct gacaaattca cacacatctc
     5761 aacaggtggt ggcgcaagct tggaattgct tgaaggtaaa gaattaccag gactagcagc
     5821 aatcaacgat aaataaacga ccaagtttgc tcaattctga cgagtaatcg atcaaaaggt
     5881 gaagggagtt ttcatgactc cctagccatt tttcaataaa ttaaggagtg tgttcctcaa
     5941 tgcgtaaacc tattatcgca ggtaactgga aaatgaacaa aactgcatca gaagcaaaag
     6001 aatttgctga agcagtaaaa aacaaaatcc cttcaaatga caaagttgat tcagtcatcg
     6061 gatctcctgc attattctta caagaattaa tgacatcagc tgaaggtaca gaactaaaaa
     6121 tctcagcaca aaactgctac tgggaaaact caggtgcgtt cactggtgaa acttcaccag
     6181 cagctcttgc tgatctaggt gttcattatg tgatcatcgg acattcagaa cgtcgtgaat
     6241 atttccatga aacagacgaa gacatcaata aaaaagcaaa agctatcttt gcaaatggta
     6301 tgacaccaat cctatgctgc ggcgaatctt tagaaaccta tgaagcaggc aaaactgctg
     6361 aatggatcga aggacaaatt acagctggtt tagctgatct ttcagctgaa caagtgtcaa
     6421 acatggttat tgcttatgaa ccaatctggg caatcggtac tggtaaatca gctgacgcac
     6481 aaatcgctga cgaaatttgt ggagttgtac gtcaaacagt tgaaaaactt tatggaaaag
     6541 aagtatcaga agcagttcgt atccaatacg gcggttctgt aaaaccagaa aatattgctg
     6601 aatacatggc aaaagaaaat gttgacggtg ctttagtcgg tggcgcaagc ttgcaagcag
     6661 attcattttt gtcattgtta gacgctgtaa aataattgtt gaaaatttgc gaaaaatgca
     6721 agaaatgttt gcactaatga gcaaaattaa ctaaaatcgt atatgaggga aatcccttag
     6781 gaaaaaacaa actcaaagga gagacaaaac atgtcaatta ttactgatgt ttacgctcgc
     6841 gagatcttag actcacgtgg taacccaaca atcgaagtag aagtatacac agaaagcgga
     6901 gcttttggcc gcggaatggt tccttcaggt gcttcaactg gtgaatacga agcagttgaa
     6961 ttacgtgacg gagacaaagc tcgttatggc ggtaaaggtg taactaaagc tgtcgacaac
     7021 gtaaacaaca tcattgctga agcaatcatt ggctacgatg ttcgtgacca aatggctatc
     7081 gacaaagcta tgatcgcttt agacggaact cctaacaaag gtaaattagg tgcgaacgct
     7141 attttaggtg tttctatcgc tgttgcacgt gcagctgctg actacctaga agtaccttta
     7201 taccactacc taggcggatt caacacaaaa gtattgccaa caccaatgat gaacatcatc
     7261 aacggcggat cacatgctga taactcaatc gacttccaag aattcatgat catgcctgtt
     7321 ggcgctccaa cattcaaaga agctctacgt atgggtgctg aagtattcca cgcattagct
     7381 tcaatcttga aagctcgcgg attagcaact tctgttggtg acgaaggtgg atttgctcca
     7441 aaccttggtt caaacgaaga aggttttgaa gtaatcatcg aagcaatcga aaaagctggc
     7501 tatgtacctg gtaaagacgt tgttcttgct atggatgctg cttcttcaga attctacgac
     7561 aaagaaaaag gtgtttacgt actagctgac tcaggcgaag gcgaaaaaac aactgacgaa
     7621 atgatcaaat tctacgaaga attagtttct aaatatccaa tcatctcaat cgaagatgga
     7681 ttagacgaaa acgactggga cggattcaag aaattaactg acgtattagg cgacaaagtt
     7741 caattagttg gtgacgactt gttcgtaaca aacactcaaa aattatctga aggtatcgaa
     7801 aaaggaattg ctaactcaat ccttatcaaa gtgaaccaaa tcggtacatt aacagaaact
     7861 ttcgaagcta tcgaaatggc taaagaagct ggctacacag cagttgtatc tcaccgttct
     7921 ggtgaaacag aagattcaac aatctctgat atcgctgttg caacaaatgc tggccaaatc
     7981 aaaactggtt ctctatcacg tactgaccgt attgctaaat acaaccaatt actacgtatc
     8041 gaagaccaac ttggtgaagt tgcagaatac aaaggtttga aatctttcta caacttaaaa
     8101 aacaaataat tcttttctaa tgaagataca tgatcgacta ttgaaagata gtcgagtatg
     8161 atcacttagt taagtaatcg aactctctac ttgctgaa
//
LOCUS       DACCKU010000108         8034 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.111, whole genome shotgun sequence.
ACCESSION   DACCKU010000108 DACCKU010000000
VERSION     DACCKU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.111"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVN13_002521"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IVN13_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7012957.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IVN13_002522"
     CDS             1228..2331
                     /locus_tag="IVN13_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7012958.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVN13_002523"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IVN13_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP7012959.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="IVN13_002524"
     CDS             complement(3821..6268)
                     /locus_tag="IVN13_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP7012960.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="IVN13_002525"
     CDS             complement(6457..7893)
                     /locus_tag="IVN13_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7012961.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2234 a         1739 c         1373 g         2688 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa ttatgtaaag
     7981 gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaat
//
LOCUS       DACCKU010000109         8023 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.112, whole genome shotgun sequence.
ACCESSION   DACCKU010000109 DACCKU010000000
VERSION     DACCKU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 8023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.112"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            259..1797
                     /locus_tag="IVN13_002526"
     CDS             259..1797
                     /locus_tag="IVN13_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP7012962.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            1794..2759
                     /locus_tag="IVN13_002527"
     CDS             1794..2759
                     /locus_tag="IVN13_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012963.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            2746..4068
                     /locus_tag="IVN13_002528"
     CDS             2746..4068
                     /locus_tag="IVN13_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP7012964.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      4131..4235
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4309..5052
                     /locus_tag="IVN13_002529"
     CDS             4309..5052
                     /locus_tag="IVN13_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7012965.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            5067..6059
                     /locus_tag="IVN13_002530"
     CDS             5067..6059
                     /locus_tag="IVN13_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP7012966.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            6071..6808
                     /locus_tag="IVN13_002531"
     CDS             6071..6808
                     /locus_tag="IVN13_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7012967.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLPDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            6798..7520
                     /locus_tag="IVN13_002532"
     CDS             6798..7520
                     /locus_tag="IVN13_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP7012968.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7604..>8023)
                     /locus_tag="IVN13_002533"
     CDS             complement(7604..>8023)
                     /locus_tag="IVN13_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP7012969.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         2570 a         1390 c         1751 g         2312 t
ORIGIN      
        1 aaaataaaag ttgtgtgtgt aaaaaagata tagctgattc caagtgacaa tttcgtatag
       61 atttttatgg tattcgtagt gaaaaaacga gaaccaaaaa gttgatccga ctttgttgct
      121 tggattcttt ttttgtcagc atatttagag gtattttttt cttttttttg ttcgtgctgt
      181 aaggttaagg caagacaagt tagttgggaa aaaggaaaga aatgtttata cttaaagaat
      241 aacagaaagg aaggtcgcat gaataatttt atacaaacat taagtgaaag aaaaggcgag
      301 ttgctggaag ctgcatttca acatctgtcc atttctctca tttctttgct gattgcagca
      361 ctgattgcta ttcctttagc gatttgggca gccaatcata aaaaaatggc agaagtctta
      421 ttgcagatca cgggtgtgct ccaaacgatt ccttcacttg ccttattagg tttacttatc
      481 ccatttgtag ggatcggaac tgttccagca ttgattgcac ttgttattta cgctttgctt
      541 ccgattttcc aaaacacgta tattggtcta gctgaaattg acccgtctat tgaagaagca
      601 gcggtagcat ttggtatgtc cagaatgaga cgcttgctga aagtagagct tcccatcgca
      661 ttacctgtca ttatctcagg tatccgaact gccttagttt taatcatcgg tacggctact
      721 ttggcagcat tgatcggagc aggtggatta gggacattta ttttgttagg tattgaccga
      781 aatacacctg ttttgacatt gattggtgcg atcagttctg ccttgcttgc aattatattc
      841 agcggactga ttcgctggtt gcagcataag aaacctcgat attccatcat tactttagca
      901 gcagtggtgt gtttatttgg cggcgtttct ttatatcagt ctgccttgtt taaaaatgaa
      961 accatcacga ttgctggaaa attaggagcg gaacctgaaa tcctgatcca aatgtataaa
     1021 gaattgattg aagacaacac aaaaacagaa gttgaattaa aaccgaattt tggcaagacg
     1081 agttttttgt tcaatgcact ggatcaaggt caaatcgata tctatcctga gttcacaggt
     1141 actgtcctag aaagcttggt gaaggtacct gaagcaatga aaagtgctga tttgacaaaa
     1201 gaaaaaacat atgacgaagc agaagaatta ttgaataagc aattcgatat gacattatta
     1261 aaaccaatgg cttaccaaaa tacgtatgcc ttggcagtca agagatcttt tgcgaacgaa
     1321 catcaactta aaaagatttc cgatttggca gctatctctg atcagatcaa agcgggattc
     1381 acactggaat ttattgatcg ttcagatgga tataaaggaa tccaagaagc atatggcttg
     1441 aatttttcaa gtgtacaaag catggagcca agtttgcgtt accaagcaat caataatgga
     1501 gatgtcaata ttgtggatgc ctattccaca gatagccagc tcaaggagta cgatctagtg
     1561 actctagaag atgatcgagg gttattccca gcttatcaag gcgcaccgct gatgaaaaaa
     1621 gattttgcta aagaacatcc tgaaatcgtt aaagcactga ataaattaag cggaaaaata
     1681 acagaggatc aaatgattga tatgaattac caagtcaatg tcgaaaagaa aaagccgaca
     1741 gaagtagcac gaatcttcct gaaaaatgaa ggattagtaa aggaggaaga tcagtgacta
     1801 aattaatcga gttcaaacat gtgcaaaaaa agtatgatgg aaagtatgtc atcgatgacc
     1861 taaatctggc aatcaaaaaa ggagaaatct ttgttttggt tggtccttcc ggtagtggaa
     1921 aaacaactac tttgaaaatg atcaatggat tatctaaacc atcagcagga gacatctact
     1981 tcaaaggaaa atcgttaaat gaatataatc tgcagaaaat gcggtggaat atgggatatg
     2041 tgctgcagca aatcgctttg ttcccgacca tgacagtcaa gcaaaatatt gaagtcatcc
     2101 ctgaaatgtt aggttgggaa aagcagaaaa gagcagaccg tgtagatgaa cttcttcaaa
     2161 aagttggctt atctcctgat atttatcgag atcgtatgcc tagagagttg tctggaggag
     2221 aacagcaacg gatcgggatc atccgagcta ttgctgcaag cccagatgtg attttgatgg
     2281 acgagccatt tagcgcatta gatccgattt caagaaactc attacaagaa ttggttttgt
     2341 ccttacacga agaattgggc acgaccatcg ttttcgttac gcacaatatg gaagaagcaa
     2401 tcaagctagg tgatcgtatt gcatttatga aggatggaga aattatccaa tgtgacacac
     2461 cagagcagtt attgatgaat cctaaaaacg actacgttcg gcatttcttt gatgaaccga
     2521 aacagacgaa agagtggcga gtagaagatt tagtcgtcaa tggatatttc ttaaatgaaa
     2581 taccagaaaa tgctcgtcaa caagtttgct ttgatacgcc tatgaaagaa gtatattcgc
     2641 ttctttcggt gtatccaagc attgtgatag tagaaaagca aagaagtatt gggtcgctga
     2701 caagtaaaga aatctttgct tttttaagtc gagaggagga aggaaatgaa aacatatgat
     2761 gcaatcatca tcggttcagg tgtgagcggc ttaagtgctg cttatggatt gaaagaagca
     2821 ggtaaaacag tactagtggt cgaagaagat ctatggggcg ggacttgtcc taaccgtgga
     2881 tgtgatccta aaaaagtctt gttaagtgca gtagaggcac gtaatcgggt aaaacaattg
     2941 agtggaaaag gttttaacga gatcccaact gccaactggg aagaacttca aaaattcaaa
     3001 cgaacattta cggatccagt cccagaaagc cgaaaaaaac aattagcaga agctgaaatc
     3061 gatcatcttt caggaaccgc tcgttttttg gatgattcat ctattgaagt caacgaagaa
     3121 gtcttccatg cggattactt agttttggca accgggcaaa gacctacgat cttaccagtg
     3181 gagggaaagg aatacttgaa aactagtgca gactttttat cattgcctgt actgcctaaa
     3241 gaaatcattt ttatcggtgg tggttatatt gcttttgagc tagctacgat tgccaatgct
     3301 gcagggagca aagtaaccat tgtccatcat aatcaacggc cattaaaaga atttgaggcg
     3361 tctttggtag aggaagccgt ccatcaaatg gaagcatctg gcattcagtt tgcttttggt
     3421 gttgaaaccc aaaagatcat ttcagaaggt actcgctatc gtttagtcgg aaaagagacg
     3481 gaattagtag cagatatgat tttttgtgcg actgggagac agccgaatac agaatcttta
     3541 gcacttgagc aagcgaatat cgtctttgat aaacacggaa ttgcagtcaa tgattatctg
     3601 caaacgagca atccaaaaat ttttgcgtgt ggagatattg tttcaaggaa aaccccaaaa
     3661 ttgacaccag tcgccacttt tgaaggaaat tatgtagcta agcggataac ggatgcaaca
     3721 agcgaaccta tcaaatatcc gattatacct acgatcgtct acgccagtcc taaacttgct
     3781 gaagtaggag ttacaaaaag tcacgcctct tcgtcagatc aagtggtaga gatggatctt
     3841 accagttggt tcacctatca tcgtgtcaat gaaccagttg ctaaggctga attgaccttt
     3901 gaccagcaaa attatttgat tggtgctgca gtgattagcg agcaagcaga cgaattgatc
     3961 gatgatttga cactcgtcat caatcaaaaa ttgactaaaa aagagttgga tagctatatc
     4021 atggggtatc cgacattggc aagtgatctc tcctatttat tgaaataatc agcatgataa
     4081 ctaaagcgct tgttgctttt tccttcttgt gctacacttt aaaaaatgaa tacctgtttc
     4141 tggggatgtc gtagacgaca tgagagtaag ttgtgaactt agacccattg aacctgtcag
     4201 ttaatactgg tgaagggaga aacatagcag acgcttgaag cgggttacta tgtcgtgccc
     4261 atttcaagcg ttttttgtta ttcatattag aagaaaggag gtaacaaaat gaagaaaaat
     4321 ctttatccag cagtcagtat ggcattcatt ttaattattt ggcagattgt tgtcatagtt
     4381 tggaaaatac ctgcctttat tttacccgga cctgctgctg tattttctgc attagcggca
     4441 gatgcacaag tattactttc ccattctgtc acgacattaa atgaagcagt gcttggtttg
     4501 gtaatagcta gcgttctttc ttttattact gccttggcaa tggattattg gaagttttta
     4561 gaaatgtctt tttatccgtt actagtgatt tcacaaatgt tgccaatcat ggtgttaggg
     4621 ccattactta ccctatggtt tggtttcggt atgcttccaa aagtgatcct tgttgtcttg
     4681 ataagctatt ttccgatcgt agtttctttt tcggatacat tgaggaaagt atcaaaagag
     4741 caaatcatct ttttgaaaac gatgggggca gatacaggga agatttatcg aatatataaa
     4801 ataccagcgg gtttatccgg cttcttttcc ggtttaaaag tggctgctac ttattgtgtc
     4861 agcggagcag tcgttggaga atggctaagc gctcaagcag gcttagggta ttacatgata
     4921 cgcgtaaaaa acagctacca gatcgataaa gtttttgctg cgatcatttg tgtcattcta
     4981 ttgagtttat tgctgaatgg cgcttgttca ttattgaaaa aagggtatta tcgtatgctt
     5041 tatatttttt aagagaaggg gatcaaatga aaaaattatg gcttttatta ccgctactgt
     5101 tgcttttatc cgcttgtggc actgcaaaag aaacaagtag caaacaagaa ataaaggatt
     5161 tgaaaaaagt gacacttgtt ttggattatg taccgaatac taatcatact ggcatctacc
     5221 tagcaaaaga aaaaggctat tataaagagg ctggtttaaa tgtccaaatc atcgaaccag
     5281 gagataattc gacaagtatt ggtctagtag gtgcggacaa agcacaattt ggtgtaagtt
     5341 atcaagaaga tgtcacttat gctcacgcag atggacagaa tataccagtc aaagcaattg
     5401 ctacagtgat caaacataat acttctggat tcgccaccct atcagacagt aacatccatt
     5461 caccaaaaga ttttgaagga aaaacatatg caggttggca aagtccaagt gaagaagctg
     5521 tcttaaaagc tgtgatggaa aaagacggcg gggacttttc aaaactgaca atggtaggaa
     5581 gcaatggaga agggccagaa agcttaggaa aatcctcaga tatccaatgg tactttgaag
     5641 gttgggatat gatcaaagca aaagaagcgg gtattgaact aaactatatc ccattgaaag
     5701 aattagatga acggttagat tactatactc cggtaattat tacgaatgat caactgatca
     5761 aaagtgatcc agaattggta cagtctttta tggatgccac gaaaaaaggc tatcaggaag
     5821 ctattaagga tccaaatgac agtgctaaac tgttgcaaaa atatgcaaaa gaaaatgatc
     5881 ggacgttttt agaagaatca caagcattct tgtcaaaaaa ttatacggat gatcctgaga
     5941 attggggatt gatggaagaa aaagtttggc gcaattatac agcttttatg caggaaaacg
     6001 gattgatcaa gcaagatgta ccttcgcaag aactattcac taatcagttt ataaaatagg
     6061 agtgatagat atgtcaatcc aaatcagcca cttgaatgta gtcattgaaa acctgaaaat
     6121 attagaagac atccatttca ctatccctaa agataccttt acatcgattg tcgcgccaag
     6181 cggagccggt aagtcaacat tgctgaaaac tttaacagga gtccagccta tgacttctgg
     6241 aacgatcaaa gtagacgatc aaaaagtaac tggtctccat acagcattta gttatatgcc
     6301 gcaagaagat atgttacttc cttggctgag cgtctatcaa aacgttacgt tatatcaaaa
     6361 aatcaatcat ctaacaatca atgaagaaca agtaagggat tatctggata tctttgggtt
     6421 aaacggctat gaacattttt tgccggaaca attatctggc gggatgaagc agcgaacggc
     6481 cttgttacga acaatcatga atccttcttc ttacttgctt cctgatgaac catttggagc
     6541 gttagatgct atgaccagag ggcagatgca agactggatg ttgaagctgc ctaaaaaagc
     6601 aaagcggact actttacttg tgacgcatga tatcgaagaa gcaatttatt tatcagacag
     6661 gattcttgtg ctttccgccc gtccggcaca tgtgattgct gagattcaag tgcctgaaaa
     6721 acgacggtca cgtgaatggc tactgcagca atcagagttg aagcaaacga tttatcagtt
     6781 attggcaggt gaattagatg ttaagtgatg ctcattgtca tttagatggg agtcagagtt
     6841 tagcactcct tcagcaggaa cacacgattt tgacgatcat caattgtgat tcgccagaag
     6901 aatggaagga aaacagacag cttgcagcaa gtaagactca agccttaagt tacggtatcc
     6961 atccttggaa agcagatagc tatacgtttg aacaagttga gccttttttg aaaaaagcaa
     7021 ggatcatcgg tgaaataggg ttagataata tttggacgaa tgtaccgatg actacacaaa
     7081 aaaaagtatt tgagcgacag cttgcttttg cagccataaa tgagaaacct gttgtactgc
     7141 acacaaaagg atgcgaaaaa gagatcttaa attatatcca agcttaccct aatcactatt
     7201 tgatccattg gtattcttct ttggactatc aacaagacta tattgattta ggctgttatt
     7261 tcagcattgg tcttgatttg aaaaagaatc cagcagtctg gcaactagct aaagcagtcc
     7321 caatcgacca cttacttatt gaaacggatg gactacatgc agcagagtgg gctttagggg
     7381 ggattttttc agaaaaagat tatgtctcac tgcttgaaga acagttaaag acaatcgctg
     7441 agatcaaaga gctatcagtt gaagaagtta aaaaaaatac ttatcaaaac ttggaacgtt
     7501 tcattagatt aggtaaataa aaaagagatg tgacacaact agtgtcgcac ctctttttgg
     7561 ctttctttgt ataaaagtaa actgtttgat taataaatga atcttagtac caaccgtttg
     7621 cttcccagaa tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg
     7681 ctactctttc ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt
     7741 aacgtccgat gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga
     7801 tccattcttt tgcagagctt gtgcttgctg gagtagcagc agtttctgtt acagcaggag
     7861 caggttgtgt ttcagcaaca ggcgctgctt gtttttccgt ttgaaccgtt tcttgtacaa
     7921 caggttgttc agcttggact ggtgcttctt gaacggctgc ttgaggttca gcagctgctt
     7981 gtgcagagcc ttctgttggg attgttaatt gttgtcctga gta
//
LOCUS       DACCKU010000110         7960 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.113, whole genome shotgun sequence.
ACCESSION   DACCKU010000110 DACCKU010000000
VERSION     DACCKU010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.113"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(244..1254)
                     /gene="trpS"
                     /locus_tag="IVN13_002534"
     CDS             complement(244..1254)
                     /gene="trpS"
                     /locus_tag="IVN13_002534"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP7012970.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            1508..2245
                     /locus_tag="IVN13_002535"
     CDS             1508..2245
                     /locus_tag="IVN13_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7012971.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            2259..3074
                     /locus_tag="IVN13_002536"
     CDS             2259..3074
                     /locus_tag="IVN13_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP7012972.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            3087..3734
                     /locus_tag="IVN13_002537"
     CDS             3087..3734
                     /locus_tag="IVN13_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7012973.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            3750..4406
                     /locus_tag="IVN13_002538"
     CDS             3750..4406
                     /locus_tag="IVN13_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7012974.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            4568..5389
                     /gene="racE"
                     /locus_tag="IVN13_002539"
     CDS             4568..5389
                     /gene="racE"
                     /locus_tag="IVN13_002539"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP7012975.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            5392..6747
                     /gene="rph"
                     /locus_tag="IVN13_002540"
     CDS             5392..6747
                     /gene="rph"
                     /locus_tag="IVN13_002540"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP7012976.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            6748..7266
                     /locus_tag="IVN13_002541"
     CDS             6748..7266
                     /locus_tag="IVN13_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP7012977.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            7373..7873
                     /locus_tag="IVN13_002542"
     CDS             7373..7873
                     /locus_tag="IVN13_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP7012978.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         2590 a         1370 c         1705 g         2295 t
ORIGIN      
        1 atgaagaaat caataaagca atggaaaaag tcgaaaaagc gttggtagaa aacctagagg
       61 caagtattcg ctaagaagga aaatatagta agtaacgatc aagtagcttt tgaagaccat
      121 acatttgaaa aaaagatgtg acacaacttc gtcacatctc tttttttgat tttaaaaaag
      181 gaatagcaca gagacgagat aaaaaaacag gcctttacag cctgcttttt tagagtaaat
      241 gaattaatgg aagtaattga ttcccattgc tgattttact tcagaaagtg tttgtgcagc
      301 tactttagca gcttcttcac tacctttacg taagatttcc ataacagctg aaggatcttt
      361 ttccaattct tctcggcgcg cacggatcgg tgcaaattca gcttccaata catcaatgag
      421 gtatcgtttg atctttacat caccaagtcc gccgcggcgg taatgagctt tcatttcttc
      481 aattgcttct ttatctgttc cgaatacatc caagtaggtg aagaccatat tcccttctac
      541 ttgtccagga tcttgtacat ggatgtggtt tggatcagta tacatactca tgactttttt
      601 ctgtaagaca tcggcaggat cggagatata aataccgttt cctaacgatt tactcatttt
      661 gccatttcca tcgattccag gtaatctacc cattcctttt ggtgggaata cgccttttgg
      721 ttccaccaag acttcaccat atgtatgatt gaagctttgt acgatttcct gtgtctgctc
      781 tagcattggt ttttgatcct caccaacagg tacaagattc gctttgaatg cagtgatatc
      841 tgctgcttga gaaacaggat agataaagaa tcctgtaggc acgctttcac cgaatttttt
      901 ttgttcgatt tctgttttga cagtcggatt gcgccgaacg cgtcctacgc tgactaagtt
      961 caaataatac atggttaatt cagctaattc cggaatttgt gattggataa ataaagtaga
     1021 ttttgcagga tctaatccca cggctaaata gtctaaagca acttgcaaga catttgatga
     1081 aaccttctca ggatttttag cattatctgt cagtgcttgc atgtcagcaa tcatgacaaa
     1141 cagttggttg tcttcatctg cctgcatagc cactcgggtt tttagagaac cgacgtaatg
     1201 ccctaaatgc agcttcccag ttggtcgatc tcccgttaaa ataatatttt tcaaaaaaga
     1261 cacctcttta cgatttgtaa gtctgagcac ttaatatctt accgtttttc ctgaaaagaa
     1321 acaacagttc ctaaggaaaa aacaaggaaa gaggataaaa taactaatag attagaaatg
     1381 gaagaaatat ttatgggaga ataaacgttc taagaaactt tacaaattac taaactatca
     1441 gatataatgt attttattat acatctttaa aagaaatctt actaaataat aatgggagtg
     1501 taattctatg gcaatgatta aatttgatca cgtggataaa tattatggca agttccacgc
     1561 attaaaaaat atcaatctcg aattcgagaa aggagaagtt gtcgttgtca ttggtccttc
     1621 tggttcagga aagagcacga tgcttcgctg tatcaatgga ttagagacga tctcttcagg
     1681 taagctgctg attaatgata ctgacttgca tgataaaaag acaaaactga cagaagtccg
     1741 aaaaaacatc ggcatggttt tccaacattt caatttgtac ccaaataaaa ctgttctgga
     1801 aaacatcaaa ttagcaccaa tcaaagtgtt gaagcaagac caggcaactg cagtcaaaaa
     1861 cgcggagaaa tttttgaaaa ctgtcaatat gttagataag aaagattctt atccttccat
     1921 gttatccggg ggacaacagc aacgggtggc aatcgccaga ggtttagcga tgaatcctga
     1981 gatgctgctg tttgatgaac ctacttctgc tttggatccg gaaatgattg gtgatgtatt
     2041 agacgttatg aaaaaattgg ctcatgacgg tatgtcaatg attgtcgtga cacatgaaat
     2101 gggctttgcc aaagaagtgg cggatcgtgt tatcttcatg gctgatggag aagtattgga
     2161 agacagcaga aatgtgagag actttttcga aaatccaaat gaagtccgag cacagcaatt
     2221 tatcagtaaa gttatcaatc attgatagga ggaaaaagat gcgcaggtta aaaaaaatac
     2281 gcctgatttt ccttttaggc attgcttttc ttgcactagc cggatgtaaa ggaaatgggt
     2341 tggcaaatga agatatttta gagcgaagca aatctaccaa tgaaatcatc tggggtgtca
     2401 agtatgatac tcgattattt ggaatgatgg atattgagag cagaactgta caaggatttg
     2461 atgtagatat tgcaaaagca atcactaaaa agatccttgg tgacaatgga aaaacagaat
     2521 ttgttgaagt cacttctaaa actcggattc cattattgaa aaacggaaat atcgatgcaa
     2581 ttattgcaac gatgacgatc acagatgaac gaaaaaaaca ggttgacttt tcagatgtct
     2641 atttcgatgc tggccaagct ttgttggtaa agaaaggaag ccaaatcaaa agtgtagatg
     2701 atttgaatgc ttctacaacg gttctagcag tcaaaggttc tacatcggct gcgaatatcc
     2761 gacagcatgc accagatgca aaaattttag aattagaaaa ctatgcagaa gcatttactg
     2821 ctttacaatc tggtcaggga gacgcgatga ctacggataa tgcgattttg ttagggattg
     2881 cagatgaaaa tccagagtat gagttagttg gtggaacgtt tactaatgag ccttacggga
     2941 ttgcgatcaa caaagggcaa gaaaacttct tgaaagctgt taaccaagcg ctagaggaaa
     3001 tgcatgcaga tggcacatac gataaaatct atcaaaaatg gtttccaaat gaaactgaag
     3061 gaaaagtaga gtaaggggga gaggcaatga gcgaattgat acaaacttat ggaccaatct
     3121 ttgtcgacgg gttcaagatc acattgtttt cgagtgtctt agcacttgtg tttagtttga
     3181 ttatcggaac gttaatggca atcttccaat tatcaaagaa taaagtagtg agcgccttag
     3241 caaaggcata tgtagaattt tttagaaaca ttccattatt aatcatcgtg atgttttttt
     3301 atgttgtcgt accgatttat tggtttaatc tagatggatt ccaagcaggt acgattggat
     3361 taacgatcta tacatctgct tttattgctg aaacagtacg ttcagggatt caaaccgtac
     3421 caaaaggtca aacagaagca ggactatctt ctggttttac ctattcagaa atcatgcgtt
     3481 atattgtttt accgcaagca ttcaaaatcg ttatcccgcc attaggcaac caatttatca
     3541 acctcgtaaa aaactcatct attttagcaa tcgtagctgg tttagatttg atgtatcaag
     3601 gagacttgat cgctagtgaa acattcaata ccttcgatac gtatatcatc gttgggttat
     3661 tttacttagt tatcacgcta ccgttatctt acttaatggt ttatcttgag aaaaaatggg
     3721 cggttcgttc atagaaggag gggaaataca tggattttag cggcgctttt tcatggatga
     3781 atatccgttt tttattggaa ggattaaagg ttactgttga agtttcattg ttttcgatca
     3841 tttttagttt cttgatcggt ggtctgacag gtgtgattcg ttttgcgaat atccctttct
     3901 tttccaaaat ccttggtgta gtgattgata ttatacggaa tcttccatta ttattgatca
     3961 ttttcttcac ttactttgcc ttacctcaaa tcggcattca gatgaacatc ttctggtccg
     4021 ctgtcgcagc attaacgatt tttgagtcag ctatgttgtc ggaaattttc cgagctggac
     4081 tcaatgctgt tccaaaagga cagatggaag caggattatc aacgggactg acttatgtag
     4141 aatcgatgcg aacgatcgtg ttaccgcaag catttaaatc aatgatcccg gcaattgtga
     4201 gccaattgat ttcactgatc aaagatactt cattagcagt aatcatttct ttacctgaac
     4261 tttctcatca ggcacgtatt atttatggac agaacaccaa ttatgtgctt ccgatgttca
     4321 tcatcatgac acttatgtac ttcgtggtat gttacgcgct ttctctgctt tccggttact
     4381 tggaaaagag acggtacagt tattaataga tgattctttt agcggttgtg gcaaactaaa
     4441 gtcacaaccg cttttcctct ttccattcga aaaaaagaga tttcgattgt tttaagcaag
     4501 taagagcact tgaaaattca cgctaaaaag tacacaatag ggaggataga atttattgaa
     4561 aaaaaggatg atacgattga cagataatcg ccctatcgga tttattgatt caggtgtcgg
     4621 cggcttgact gtagtaaaag aagccctgaa acaattaccg aatgaaaata ttttatttgt
     4681 aggagacaca gcacgctgcc catatggccc tagacccgcg gaacaggtaa tccagtatac
     4741 ttgggaaatg acggattatc tggtggagca aggaatcaag atgctggtga tcgcctgcaa
     4801 taccgcaact gcggtggctt tagaagaaat caaagctgct ctttctattc cagtcatcgg
     4861 tgtgatcctt cccggtacta gagcggcagt aaaaaaaaca caaaataaac aagttggcat
     4921 tatcggtacg attggtacgg taaaaagtca agcttatgaa aaagcactga aagagaaagt
     4981 accagaattg actgtgacaa gtcttgcttg tcccaaattt gtttcagttg tcgaaagtaa
     5041 tgaataccat tcatcggtgg cgaaaaaaat tgtggcagaa acattagctc ctttaaccac
     5101 taaaaaaatc gatacattga ttttgggatg cacccattat ccattattac gccccatcat
     5161 tcaaaatgta atgggagaaa atgttcaact gatcgattct ggagcagaaa cagtaggtga
     5221 agtatctatg ctgttagatt atttcaattt gagcaattca ccgcaaaatg gtcggacatt
     5281 atgccagttt tatacaactg gctctgccaa acttttcgag gaaatagctg aagactggct
     5341 tggaatcgga cacttaaatg tagaacatat cgaattggga ggaaaataag gatgcgtcat
     5401 gacgggagaa aagcccaaga gcttcgcaaa ctggagcttc aaacaaatgt tttcaaatat
     5461 ccagaaggat cggtagtgat ccgttttggt gatacaacag ttatttgttc tgcaacaata
     5521 gaaaattctg ttcccccttt tttgagagaa acaggaacag gatgggtgac agcagaatat
     5581 agtatgctgc ctcgagcaac gagtacgaga aatcgtagag aaagcagcaa agggaaactt
     5641 tctggtagaa caatggaaat ccagcgtctt attggtcgtt ccttgcgtgc agtagtagac
     5701 ttggaaaaat taggagaacg tagtatcatc gtagactgcg atgtgatcca agcggatggc
     5761 ggtactagaa cagcaagtat cactggggca tttgtggcat tgaaattagc cattgaaaaa
     5821 cttttaagag aaaaagaatt gtcagaagat ccaataaaag aacatcttgc tgcagttagc
     5881 gtaggaatct taccagatgg gacttgcgtc acagatttgg attaccaaga agattccgca
     5941 gcacttgtag acatgaatct tgtaatgaca gaatctggaa aattcgtgga gattcaagga
     6001 actggagaag aagcaacgtt tgatggtgaa cagttgaatg aaatgctctt ttttggaaaa
     6061 aacgcgattg aagatttgat caaagagcaa aaacatgctt tgctaacaga gtttgctcaa
     6121 aatgatgagc ggatagaaga aacaaaaaca atcatcattg cgacaagaaa tccaggaaaa
     6181 gcagaagagt ttcgaaatat gttcaaagaa gcgggatatc atgtaaaaac attacttgac
     6241 tatcctgaat tgccagatgt ggaagaaact ggcagcacat ttgaagaaaa tgcccgatta
     6301 aaagcagaaa cgattgcaca attgcttgat cagccagtct tagcagatga ttcaggtttg
     6361 aaagtggatg ctttaggcgg tatgccagga atctattctg cacgttttgc aggggaacaa
     6421 aaaagtgatg cggggaataa cgccaaatta ttatatgaat tgacagatgt gccggatgaa
     6481 aaaagaacag cacaattcca ttgtacgtta gtattcgcgg cacccaaaaa agacagcctt
     6541 gtagtagaag cggaatggcc cggtcgagta gcccgtatac catctggcga aaatggcttt
     6601 gggtatgatc ctttatttat cccagaaggt aagaaacaaa cagcagcaga attatcgagc
     6661 gaagaaaaaa acaagatcag tcatcgggct caagcaatga aaaaattgag tgccgagtgg
     6721 aaacaatggc tggaaggaga gagatagatg cggtatttag tagtaagtga taatcatgga
     6781 gacagagata tcctcgttga cttagtagat cgttatcgtt ctgaagtgga tatctttttc
     6841 cattgtggtg attcggaatt agaagcttct gacccattat gggatacatt ccaagtggtt
     6901 caaggtaatt gtgattatgg accaggattc gaacagaaaa aagtgatcca aactggtcag
     6961 gatacagtat tcatgactca cggtcattta tcgaatgtcc gatttggttt gacacagtta
     7021 gcaatcgaag ctcgagctgc aactgccgat atggtgtttt ttggtcatac ccatcaaata
     7081 ggctgtgaga tggatcagaa aatcctgttt ttgaatcctg gtagcatttc tcagcctaga
     7141 ggcccgatcc aaatcccatc atatgctctt atcgaaagtt tagatgacca aatttctgtt
     7201 caatactata accgtgccca tcaggcagtt gaagagttgg ccttttcttt ttcgagagag
     7261 cgctgaataa aaaaactcta atttttaata aaaataagct ttattttatg aattttgcat
     7321 ccggtttcgg taaaatagta gtgttgaatg aaatagaaat ggaggccatc agatgattgg
     7381 acctatagta agagaaatgc tgctggaaaa tcaggaaacg tttttgattc ctgcagaaaa
     7441 tgtggcaaat gttatgtatc atcatccatt gtcccatggt ttacttgtgt tatcgaaggt
     7501 cggttattcc aaaattcctg tattaggaaa agatgatcac tttgtaggac tagtaagttt
     7561 atcagatgtg gtcaataaaa tgattgactt gcaaacaatc agcatggacc cgttagaagg
     7621 actaacggtt gctgatgtga cagaaacaga tgttccgaca attgatgaaa attgggaact
     7681 agaagaagta ctccatttgt tagtcgattc gcctttttta ccggttgtca cagaagatcg
     7741 gattttcaaa ggaatcatta caagaaaaga gctgcttaaa gccgtaaact tcatggtgca
     7801 tgaattagaa cgtcgtaata tattgtctcc gaaaatcgaa gaattaaaag aacaaatcga
     7861 tatcgtgagc taaagaacga gaaactaaaa gataatcaaa aaataagtag aaaaaacagc
     7921 gtaccagttt tgactttatc ttgaaaaaga ggctgggaca
//
LOCUS       DACCKU010000111         7621 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.114, whole genome shotgun sequence.
ACCESSION   DACCKU010000111 DACCKU010000000
VERSION     DACCKU010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.114"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IVN13_002543"
     CDS             complement(105..629)
                     /locus_tag="IVN13_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012979.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVN13_002544"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IVN13_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP7012980.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVN13_002545"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IVN13_002545"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP7012981.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IVN13_002546"
     CDS             complement(2351..3031)
                     /locus_tag="IVN13_002546"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP7012982.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IVN13_002547"
     CDS             3157..4026
                     /locus_tag="IVN13_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP7012983.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IVN13_002548"
     CDS             4052..4567
                     /locus_tag="IVN13_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012984.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IVN13_002549"
     CDS             complement(4614..5177)
                     /locus_tag="IVN13_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7012985.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5387)
                     /locus_tag="IVN13_002550"
     CDS             complement(5232..5387)
                     /locus_tag="IVN13_002550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012986.1"
                     /translation="MRKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIVTHGYFITE
                     KIEDFLK"
     gene            complement(5487..5996)
                     /locus_tag="IVN13_002551"
     CDS             complement(5487..5996)
                     /locus_tag="IVN13_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012987.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6092..6472)
                     /locus_tag="IVN13_002552"
     CDS             complement(6092..6472)
                     /locus_tag="IVN13_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP7012988.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6802..7551
                     /locus_tag="IVN13_002553"
     CDS             6802..7551
                     /locus_tag="IVN13_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP7012989.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2168 a         1580 c         1354 g         2519 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt tccgcaatta gggcatttca
     5401 ttttttccat gttatctcaa cttcctttca tctttactct cttagcttac tcttatattg
     5461 taaaaaagag aagcaatatc cttcttctat gcagatatcc atacctgacg gtcctgttct
     5521 ccatctatta atcggacttt ataataagga cgttcatctt tgactagaag tgtccactct
     5581 ttcacttttg cttttttccc gtagtagctg actgcttcct tcatcgctgc ttgcggttgt
     5641 ttgaactctt ccgttttttc tatatcaaaa agatgttctg cggccttttc cgtgtcattt
     5701 cgtacggttg ttttcccagt ggatggattg actgctatga cttgtccttc acaaaaaaac
     5761 tggtattcat atgttgcatt cgtaggattc tccaaatctt tttcaactgc aaaatgaata
     5821 gcagatatgt tatgttcttt tgtttcgttt tttacgattg ctgctgcttt tttcaatgag
     5881 atccgatatt cctggtcact aatatcatcc atgtgaaaag cggcattgag cagtcccgtc
     5941 ataccggtaa tccctattac gatgagggca gcaacaccaa acatcatttt tttcattctt
     6001 cattctcctg tttctttttt ttaaagaata tcaagaaatg aacgaatgaa aaatcgaatg
     6061 aaacgaaaaa aaagtgacaa ttttgttatc tttattcgtg cagttctaat ggcaatccgt
     6121 ctggatcaaa gaaaaacgtc attttttttc ctgtaaaggt atcttctcta atcggttctg
     6181 attcgatccc taaggactgc aattctgcaa ttgtttcttc aatatcgtct actttgaaag
     6241 caagatgacg caagccacaa gcttctggat agtttggtcg ttgtggcgca ttagcaattc
     6301 caaaaatctc caattcagca cttcctagtt tcagatctaa tttatagtca tctcgttctt
     6361 tgcggtaatt ttctctgatt acttcaaatc ctaatttatt aacataaaaa tcacgtgatt
     6421 tctgataatc tgaaacaatg atggctatat gatggatcgt tgtaaggttc atatgagcac
     6481 tcctattctt cgtttaagct cggatctata ttaatctcca gttccattgt aaatgtgtca
     6541 gattgaaaaa aatagctgtt tttccttttt ataagaaaaa aggcaaaaaa aacagaaagt
     6601 aggtcctcca agacactctt cctgttccca atataataaa tgaaagtaag aaattatatt
     6661 ttcaagaaaa caaacagaaa attatcataa cacaaattat ttataattga aagactagtt
     6721 ttttttcaag aaaagcatac aatagattgt gttagtaaat acagaacaca ctacatttag
     6781 tgtcttaagg agtcctcata catgaagtca tattttcagt ttttagctca tcagctaaaa
     6841 ggctggccgc aacaaaatta ttatcttttc tttttcagtc ttggctgcca ggtcatgact
     6901 cttgtcaata gtcccatcac ttggctgtcc gttattacat ttatcggtac cacgcttggt
     6961 gtattgtgta ttttatccat caatgcggca aaatcagtca acggtatttt aggtattttg
     7021 tctgcgattt gttttattat cgttgggttt tcagcaaaga actatttaag tatcggtgaa
     7081 cagattgctt atatgatcac attggatatc cccgtattgt taagtaaaga atggaatcat
     7141 aatatggctt caaaaatccg aaaattcact ggaaaaagct ggtttatcgc aatcatcagt
     7201 actttccttg tctatcttgt ttcaggattc ctgattggtc gttatacaga tgatcctcgg
     7261 ccgtgggtag atgccattgc tttttctatt agcttgactg ctggtatcat cagtttcttg
     7321 cgttacaaca accaatatta ctggtggtta gcttcagggc ttgctcaaat ggtcctttgg
     7381 tatatttcat ttacgcatgg atctgcaagt ttggcgatgt tcgtgaacag ctctgtttac
     7441 ttgctgaatg atgtccttgc atttaccgtt tcaccttggt ataaccaaaa agaacgtgaa
     7501 cgtatgcaaa aaatggaaga agaatactac aacgcacaac aacacgcata aaaaaactga
     7561 gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
     7621 a
//
LOCUS       DACCKU010000112         7600 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.115, whole genome shotgun sequence.
ACCESSION   DACCKU010000112 DACCKU010000000
VERSION     DACCKU010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.115"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IVN13_002554"
     CDS             complement(138..428)
                     /locus_tag="IVN13_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012990.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IVN13_002555"
     CDS             complement(546..806)
                     /locus_tag="IVN13_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012991.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IVN13_002556"
     CDS             complement(943..1356)
                     /locus_tag="IVN13_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP7012992.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IVN13_002557"
     CDS             complement(1812..2297)
                     /locus_tag="IVN13_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7012993.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IVN13_002558"
     CDS             complement(2436..2651)
                     /locus_tag="IVN13_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP7012994.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IVN13_002559"
     CDS             2875..3675
                     /locus_tag="IVN13_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP7012995.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IVN13_002560"
     CDS             3694..5562
                     /locus_tag="IVN13_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP7012996.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKVGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="IVN13_002561"
     CDS             5555..6046
                     /locus_tag="IVN13_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAP7012997.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="IVN13_002562"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="IVN13_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP7012998.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="IVN13_002563"
     CDS             6606..>7600
                     /locus_tag="IVN13_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP7012999.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1444 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aagtgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DACCKU010000113         7567 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.116, whole genome shotgun sequence.
ACCESSION   DACCKU010000113 DACCKU010000000
VERSION     DACCKU010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.116"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(89..1090)
                     /gene="ccpA"
                     /locus_tag="IVN13_002564"
     CDS             complement(89..1090)
                     /gene="ccpA"
                     /locus_tag="IVN13_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP7013000.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            1300..2403
                     /locus_tag="IVN13_002565"
     CDS             1300..2403
                     /locus_tag="IVN13_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP7013001.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGADSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVVWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(2465..3067)
                     /locus_tag="IVN13_002566"
     CDS             complement(2465..3067)
                     /locus_tag="IVN13_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP7013002.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSAELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(3070..3510)
                     /locus_tag="IVN13_002567"
     CDS             complement(3070..3510)
                     /locus_tag="IVN13_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP7013003.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIIRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            complement(3636..4574)
                     /gene="galU"
                     /locus_tag="IVN13_002568"
     CDS             complement(3636..4574)
                     /gene="galU"
                     /locus_tag="IVN13_002568"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP7013004.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKIELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            complement(4589..5614)
                     /locus_tag="IVN13_002569"
     CDS             complement(4589..5614)
                     /locus_tag="IVN13_002569"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP7013005.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNHYFRIYTNPDVVGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(5658..6488)
                     /locus_tag="IVN13_002570"
     CDS             complement(5658..6488)
                     /locus_tag="IVN13_002570"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP7013006.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLLVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            complement(6503..7441)
                     /locus_tag="IVN13_002571"
     CDS             complement(6503..7441)
                     /locus_tag="IVN13_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP7013007.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         2034 a         1659 c         1363 g         2511 t
ORIGIN      
        1 aaaattttgt cataacccct tttctcgaat aaacgttgtt ccagaagttg gccacttttg
       61 gaacaacctc taaatgattg atcttttatt atttagttga tcctttttct tcgattccat
      121 aaggcaagat aatcgttttt tcttcgactt cttctttatt catcattttc gtcaataggc
      181 gcatggatac cgctcctaaa tcgtataatg gttgtgtgat acttgtcaaa cgaggacgag
      241 ctacttctgt cagtaaagaa ttgttgcttg tgatgatttc gaattcttct ggtactttta
      301 cgccacggtc taataaacca tccaagatac caatggctaa ttcatcgtct gaaacataga
      361 cagctgttgc accgctatta tggatccggt caactagaga cagaccatct ttaaagttgt
      421 attgtgcctc aaagattaat ccttcgctat atggtagatt gttttcgctt aatgcttctt
      481 tatagccttt tagacggttt tgaccattga taggatcaat cagcgcaccg ctgacaaaag
      541 caattttttt attgccgctt tttgctaatt tatttactgc atcttttgtt gctgatacat
      601 aatcaatgtt tacgctacct acttgttcat ctggatcaat ggaaccagct agtacaacag
      661 gtgtttttga acgtgaaaat tctccacgga tttcgtctgt gatatggtga cccataaaga
      721 tcacgccgtc cacttgctta gccaataaat tattcaatac attgacttct ttttgatcgt
      781 tgccatctga gtttgctaag atgatattgt atttgtacat tgttgctaca tcgtcgatac
      841 cacgtgctaa agaggcaaag aacatattgc tgacatcagg aatgattacc ccaacagttg
      901 ttgttttctt acttgccaag ccacgtgcga ctgcatttgg acgataatcc aagcgatcga
      961 taacttccaa gacttttttt cttgttgctg gtttgacgtt tggattgcca tttacgacac
     1021 gtgaaacagt agccatcgaa acatttgcct ctctcgctac atcatagata gtgattgttt
     1081 gcttttccat ttgtgttctc ctattcatat tttatatgtt ttctgaaaat acgtcttaca
     1141 tttctaaaat aggatatcaa ataatctcaa caattgcaac cgttttatct ctgttttcat
     1201 gaaaattttt acttttttca gttttaagaa cgttggtctt ttaatcaatc agtggtacaa
     1261 ttattgaata caaaattaat ttagaaagta ggttgccaaa tgaataacgt aaaaatcaat
     1321 gaactacgtc aatggatggc acaacaccaa gtagaattgg cttatatcag cgaccctggc
     1381 cacatcgctt acttttccgg atatgaaagt gatcctcacg agcgtgtact agctcttttt
     1441 atccctcttg aacaagatcc tttccttttc actcctgctc tggaagttga agatgctcaa
     1501 aacagcagct gggcgtacga tgtacgtggc tacttagaca gcgaaaaccc ttgggagatc
     1561 atcggaaaag agcttcgcgc acgctatggg caacctggta atgtagggat tgaaaagaat
     1621 gctctgactg tcgatcgctt cgatgctatc tctgatatct tgacaggtgc agattcttac
     1681 actgatctaa cttctattat ccagcgtctg caactgatca agacggtaga agaaaaagac
     1741 aaactgatcg aagctggtaa atgggccgat gttgcctttg aaattggctt caaaacagta
     1801 aaagaaggcg tgactgaaca agcaattatc gcagaaatcg aataccaatt aaaaaaacaa
     1861 ggtgtttcac aaatgtcttt cgacacctta gtccttgcag gagcaaatgc tgcaagccct
     1921 catggtacac caggaagcac gaaggtttca ccaaatgaac tagtgttatt cgatcttggc
     1981 gttgtttgga atggctattg cagtgatgct actcgtacag ttgcttatca aaaaccaaca
     2041 gaattccaag aaaaaatcta caacatcact cttgaagcac agcttgccgc acaagaggca
     2101 gttcgcccag gtgtgacagc aggtgaactt gatcaaatcg cacgaaacgt aatcaacagc
     2161 tatggctacg gcgaatattt caatcaccgt ttaggtcacg gaattgggac aaccgtccac
     2221 gaattccctt ctcttgtaga aggaaacgac ttagtgatcg aagaagggat gtgcttctct
     2281 cttgaaccag gaatctatat tcctgaaaaa gtaggggtac gtatcgaaga ttgtgtgtat
     2341 gtgacaagtg atggttgtgt gccattcacg actacaccaa aagaattact tgttttagag
     2401 taatgaaaat aaaaaaggtg ggagctggtc cgctctcacc tttttattct agaaatgagt
     2461 attcttagtg attggtagtc gaatctgtca ttgtatcttt catttctttt ttagcttctt
     2521 ttgcatcttc ttttgccatt ttcaacattt cttttgtgtc atctgcaact ggttttgctt
     2581 ctgacattcc ttcattgacc gtctctcctg ctttatctgc agcatctttt acatcgatca
     2641 tgatatcttc tgaagtatct ttagcaatac tacctaattc gtctgctggt tttttcagat
     2701 cttctgctgt ttttttgaaa cgattagata aatcagctga ttgttctttg aggtttaaca
     2761 agatatcatc tgattcactt cctgcttcct ctgctgattc acttaccttt tgcttaacat
     2821 tacctgctag atcccctgct tgttgagaaa gatcttgcgc tttttctttt gcaatgcctg
     2881 ttaactcagc tgatttttct tttgccatat ctgtgtaatc tgaagccatg tccatcaaat
     2941 catctgcttg agcagccaat tcatcccgaa gttctttacc agatttgggt gctaacaata
     3001 atgcggcgat tgctgcggct gtacctccaa ctactgcacc taataaaaat ccaccttttt
     3061 tagccattct tattttcctc ctgtcgtttc agtatgtttt ttgtttttaa aaaatttcat
     3121 cgctgttcca ccgacttttc cggcaacagt cgctttagct gtcgtttttc cgattccgcc
     3181 gactttcgtc acgatgtttt tactagaatg attaagttcc gatacacttt cacttaaatc
     3241 agcaactgct gtgaacaatg gatcgatcgt tgcgactttt ccattgatat ccaagagcag
     3301 ctcattactt ttgaccagca agccttctac ttgtctagat aaaagatcta cgtcttttgt
     3361 gacgatctca attgtcgtat ttgcttctgc gacagtctgg tctacttttt ctaaagtttt
     3421 ggaaacttgc aacaatacgc gaatgatgaa aacgacaagt atcgcaaagg cgattgctgc
     3481 gatcaatcct gcaatttctc ctcctgacat gatcaaactc ccttcttgtc tctcttttct
     3541 ataaggatag cattttcgtg ttttttaaac aaataaaaac gagcttcaac tactttttat
     3601 tcaattagca gctgaagctc gttagaaaaa aatgattatt ttttcttttc ttctttgatt
     3661 tctgttattc cttttggatt atgatcgatc ttgcttcctt ctgcttctaa tgttttacct
     3721 aaagacatga tgtattcacg caaaggtgcc cctacttctg gatgtgtcag accatattca
     3781 atacttgttt tcataaaacc aaatttgtca ccaacgtcat aacgtttacc tgtgaattta
     3841 cgggcaaata cgcgttgtgt tttattcaac gtatcgattg cgtcagttag ttggatctca
     3901 tttccagcac ctggttgttg ttctgataat acatcaaaga tttctggagt caataagtaa
     3961 cgcccaataa tagctaaatc acttggtgct ttgcttggat ctggtttttc aacaaagttt
     4021 ttgacattgt ataacccatc ttccagtact tcccctgggt tgataattcc gtattttgaa
     4081 gtttcatcgt gaggaacttg cataacggca atcgtagaag cgtgtgtacg ttcatagtca
     4141 tcgatcaatt gctgtgtcaa tggtacttcg tcttccatga tatcatcgcc tagcataacg
     4201 acaaacggtt catttccaat aaaggctctt gcttgaagaa cggcgtgtcc taaccccttt
     4261 ggatgagatt gacggatgaa atgcaagttg acatctgtcg tttcttctac taatttcaat
     4321 aattcgattt ttcctttttc acttaggttt gtttctaatt cgatatttga atcaaagtga
     4381 tcttcgattg gtcgtttagc ttttcctgta acgatcaaga tgtcttcgat tcctgatttc
     4441 aacgcttctt caacgatgaa ttggatcgtt ggtttgtcca cgattggcaa catttccttt
     4501 gccatcgcct ttgttgctgg taaaaatctt gttcctaatc ctgctgctgg aataactgct
     4561 ttttttactt tcatatacat tcgctccttc aaaaaattgt taatgagaat tcattttctt
     4621 ttttcccatc acgtaacatg atttcttttg cggctgtttt gatttctttt tgttcataca
     4681 gcacttcata gattgtttga gtgatgggca tttcgatttc catcatttgg gctagttcca
     4741 tcgctgcttg agtagtattc acaccttcaa cgaccattcc catattggct agcacctctt
     4801 ctagaggctg tccttgtcct aacaagttgc ctgcgcgcca gtttctggaa tgaacacttg
     4861 tgcaagtaac gactagatcg cctactccgc ttaagccaat aaaggtcagc gggttggcac
     4921 ccattgctac acctaaacgg ctgatctcag ctaatccgcg cgtcatgatt gccgcttttg
     4981 cattatcgcc aaaccctaag ccgtgaatag caccagctcc aatggcaata atatttttca
     5041 aagctgcccc catctctaca ccaaccacat cagggttagt atagattcga aaataatggt
     5101 tcatgaacag ctcttgtaca tattttgctg cttttgcatc tgtgcttgcc gctgtgatcg
     5161 tcgtaatgtc atgctttgct acttcttccg catggcttgg cccagaaaga acagcaacag
     5221 cttttctttt ttctgtcggg atttcctcta acaagatttc agagattctt ttatgcgttc
     5281 cctgttctaa tcccttactt gcgtggatga tcactggctt cgtattcaat ttggcaacta
     5341 gttcttttgc aacagaacgt atcgctttcg tcgggataac gaatagtacc gcatctgcat
     5401 cttttactgc atcttccata tcggtgaatg ctcggatgga atcgggtaga tgatgatctg
     5461 gtaaataacg ttgattggtg tgttgggtgt tgatttcttc tacttgtgaa gcacgatgtc
     5521 cccaaatccg aacgtcatgt ccgttttcag cgaggacttg tgctaaagca gtcccccaag
     5581 agcctggtcc taaaaccgct actctataat tcattttacc actcctgaaa tttttttcat
     5641 ttttaacgct ctaaaaatca aataatttgt tgattttttc ctaatgagcg gtcataatcc
     5701 ggtaaatttc ctttttttct acgccaaata aacagaatca atgtgccgac aagtaaaaca
     5761 gcagacaata attgagaaat gcggatcaca cctaaaaagt aaaggctgtc tgtgcgtaat
     5821 ccttcgataa agaaacgtcc gaaactatac cacattagat aacataaaag gacttctcct
     5881 tttttcaaaa gatgcggttt cttccgcaaa accaaaagca gaataaatcc aatcaaactc
     5941 caaagtgatt cataaagaaa ggttggttgt cgatagatcc cttcgatata catgttatca
     6001 atgatgaatt gcggtaaatg cagactttct aagaaacttc gtgtcgtgtc tggaccatac
     6061 gcttcgtggt tcatgaaatt tccccatcgt ccgattgcct gcgccagtaa tacacttggt
     6121 gcagcgatat ctaaaaaggt ccacaaagaa ataaaatgat atttggtaaa aaagtacagt
     6181 gtgattgcac ctgcaatcaa tcccccgtaa atcgctaatc ctcctgaacg gatattgaat
     6241 atttggattg gatcagctat atattgggga agatcgaata aaacataata caaacgggca
     6301 ccaataatgg aaatcggcaa tccccacaac ataaaatctg tcacgtcatc tgcttttaat
     6361 cctacccgaa cagcttctct gctgctgagc cacatggcaa taatgattcc tgatacgata
     6421 atcaatgcat accagtagat aggaattcct acaagctcaa aagcaactcg attgatttgt
     6481 gctaacatat tgtcactcct tgctagtcat tgcctgagtt ttcttcaatc aagcgactta
     6541 atctttcttc aaatgttttc gttgcgtcaa agcccattgt acgcgcgcgg aaattcatcg
     6601 cggctacttc aatgatgatt gcaacgtttc gtcctgtttt gacagggatt tttatttgtg
     6661 gaatgcccac ttccgcgatt tcaacggtcg tgtcttcaga acccaaacgg tcgtatttct
     6721 tatctttcgc ccatgcttct agataaacag ctaactggac ttgcatagag cctcgtaccg
     6781 cactcgcacc gaaaagattc attacatcta tgatcccgat ccctcgaatc tcaatcagat
     6841 gttccaggat tttaggaggt tctccgatta ccgtcagttc atcttgctga tagacatcta
     6901 ctcgatcgtc tgcgatcaat cgatgtccgc gcttaatcag ttccaacgcc gtttcacttt
     6961 tccctatccc actgtcccct tgaattagta cacctaatcc gtagacatcg actaacacac
     7021 cgtggacact tgttcgaggg gctaagcgcc catctaaata actagataat tcacctagca
     7081 gccgagacgt tgagataggc gaacgcagca cagctatccc attttcattg gctgcttgga
     7141 tcatttcttc cggagccggc aatcctcttg aaataataaa tgcgggcata tcttcactac
     7201 ataatcttct catgatgatc aaacgctctt ctgggatcat ccgttccgca aaactgattt
     7261 ctttactgcc aaatagctgt aaacgattat gagaataata attaaaataa ccggtcaact
     7321 ctagtcctgg gcgtgagatg tccccagtca cgatgacgcg gtctaaactt ttctcgtcgc
     7381 ctgtgacgat ttccaaagat aattttttta ctaattggct aacttttaca acttctgaca
     7441 ttcatgctac ctcatttcta ttagagtgtt ctctaccatt ttatcattgt ctagccaaaa
     7501 gtactaccaa ttaaaaaaaa cgtttttatt taattaccaa tgtaatagaa aacgcattct
     7561 taattat
//
LOCUS       DACCKU010000114         7566 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.117, whole genome shotgun sequence.
ACCESSION   DACCKU010000114 DACCKU010000000
VERSION     DACCKU010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.117"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            369..878
                     /locus_tag="IVN13_002572"
     CDS             369..878
                     /locus_tag="IVN13_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP7013008.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            978..1628
                     /locus_tag="IVN13_002573"
     CDS             978..1628
                     /locus_tag="IVN13_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013009.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            2079..3017
                     /locus_tag="IVN13_002574"
     CDS             2079..3017
                     /locus_tag="IVN13_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP7013010.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            3030..4082
                     /locus_tag="IVN13_002575"
     CDS             3030..4082
                     /locus_tag="IVN13_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7013011.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            4348..4983
                     /locus_tag="IVN13_002576"
     CDS             4348..4983
                     /locus_tag="IVN13_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP7013012.1"
                     /translation="MIFLQKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSWNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            5216..5620
                     /locus_tag="IVN13_002577"
     CDS             5216..5620
                     /locus_tag="IVN13_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP7013013.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            5753..6796
                     /locus_tag="IVN13_002578"
     CDS             5753..6796
                     /locus_tag="IVN13_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013014.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            6811..7374
                     /locus_tag="IVN13_002579"
     CDS             6811..7374
                     /locus_tag="IVN13_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP7013015.1"
                     /translation="MITVVHKERQEKSSSDVQLWVYLGSISTKIQYGYTDSAKKKGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
BASE COUNT         2498 a         1152 c         1571 g         2345 t
ORIGIN      
        1 cggcgaaaaa aataggcata ttaagcgggc taggaggaga aatattcaca tatcattttt
       61 tcattccgca agctgcgtca agttatttgg aatttgtaga acagatgaga gaggtagaag
      121 cagcactaca aactactttt caataagtag gaattataga aattttactc aaaaaatctt
      181 cattaaaaaa tctgactaaa taagttgaga aaacagaata tacttcaata tccagtgatt
      241 tataaatatc attcactcat tacccagcgc cttatcataa gtgatgcggt ttttatcatt
      301 gatcctaaaa atcttagcaa gacaaaaagt acgtaagaaa gtaaagacta aatacttcgg
      361 aggaacaaat gaataacaat gataggttat tgcgattgcg ttatgcgatt gatttgaaag
      421 atacggattt gattaaagct tttgagctag gcggtgtacc actgactaaa gaagaagctc
      481 aggcggtact gaccaaagta caggataaga acaaagataa tgcagaaaat aatgtgtatg
      541 aaaaaaccat caacaaccaa gtttttgacg cattcctaaa tggcctgatt ctcttggcgc
      601 gtggtccgca aaaagataag ccgatagtag acacgccgca aaaaagcaag gaaatcaaat
      661 atatcaacaa tgtgttgcta aaaaaattaa aaatcgcctt gtctatgacc actgatgata
      721 ttttagacgt ttttgctgag gcagaaatct atccatcaaa aggcgaaatc ggggcatttt
      781 tgagaaaaga aggccaacga aattttaagc cttgcggaga taaatatatg cgtaattttc
      841 tcaaggggtt aggaatttat aatcggagaa aagtataaag aaatttcatg agaaatcaaa
      901 acaatggttg ttttgatgag agctagtttt tgtatgagat taaaatatta tcttaatcga
      961 ggagctggat tggttttatg aatgatgaat ataagaatga tgaagataag atgctttttg
     1021 aagagataga gaatcgatgt aggctcaatt ttgagcttcg gggaaagatg tctttaatac
     1081 aacaaaaaaa gtatttagcg aataaatcag agtttactct gggacatgtt gaaaaattaa
     1141 tctcggattg gattagtagt agaagcgaat ttacaaaaat aaaacagccg attaaatttg
     1201 acatgaaaaa acttttgtta aataagagtg agataggtaa tagagatcaa tatattagag
     1261 ctaaagggca agaaataatt gattcattag gtgaaatgag gtcatacaat tatttgtatg
     1321 taacacatag agctgatgga atggtcatta ctgtggggaa atcttcttct aatgatattt
     1381 ttttagatgg agatcttttt tatcaactca atataaatca cttgtctggc actgaaaata
     1441 taattttaag gacggaatat ggcaatgaaa tatttgcaaa atatgatgag attttaaaaa
     1501 attatttaga ctgggcttgg ataattccgg ttgagtcagg agatgctaag aaactggaga
     1561 gattattggg cgatgaacta attaataaaa aagtaccaat tttaaattat tattcccata
     1621 gacaataaaa ggctattttt catgataata ctagttataa ctgtaaaaat ggaagaaaat
     1681 ctgggaaaga agtttttaaa taagtgtatt gttcgatttt aataaataat aagcaatacc
     1741 atccgtctgt gttgcgcaac atcgtactac aatgaatatt ttttcacaaa aagaaaatca
     1801 tccatcgaat gaatggaagg ttttagtata cacgaggtta atgccccctc ttttataatc
     1861 agaggagtga gattttttct atgaatgttt ctattttctt attgctagtc tagtttctct
     1921 tacgatgaaa ttgggattct attccagttt ttattgattt gctgataaaa taacttgaat
     1981 ggtggatata aacatgataa ataagagaag aataacaaaa cgaggttgtg aaaagattct
     2041 caaatgagta taatagaaag aaaaaggagg aagaatcaat gaaaatagca attgcaggcg
     2101 ctggcgcaat gggaagtcgc tttgggttga tgcttcacca aagtgggaac gaagtattat
     2161 taatcgatgg ctgggcagaa catgtccaac aaatcaaaga acatggatta caagcaaatt
     2221 ttaatggtaa agaggtagaa gcaaaactac caatcgttct tcaatccgaa gtagaaaaag
     2281 aagatcaagt tgatctgatt attctattta ccaaagcgat gcagctggaa aaaatgctgc
     2341 aggatatcca atcattaatc aaaaaagata cagaggtctt atgtctatta aatggtatcg
     2401 ggcatgaaga tattattgag aaatttgtac caatggaaaa tatctatatt ggaaatacca
     2461 tgtggacggc aggtcttgaa ggtcctggtc aggtcaaatt atttggaagt ggttcagtag
     2521 aattacaaaa tttaggtgat ggaaaagaag cagctgcaaa aaaattagca gacaaattgt
     2581 ctgaatcagg attaaatgct catttttctg acaatattca ctattctatt tatcgcaaag
     2641 catgtgtgaa cggaacaatg aacggactat gcacaatctt agacgttaat atggctgagc
     2701 taggaaaaac atcgactgct cataaaatgg tggcgacgat tgtcaatgag tttgccaaag
     2761 tagcagcagt agagaagatt gaactagatg tcccagaagt cattgcacat tgtgaatctt
     2821 gttttgaccc ggaaacaatt gggttacatt atccttcaat gtatcaagac ttgattaaga
     2881 accatcgatt aacagaaatc gattatatca atggcgcaat ttctagaaaa gggaagaaat
     2941 atggtgttgc gacgccttat tgtgatttct tgactgagct tgtccatgca aaagaagata
     3001 gtttgaacgt aaaataaagg agagattgaa tggaaacttc acaaaaaata tcaccgaaaa
     3061 tttttctgaa taaagtcctc gctggtacag caacaggaat cattattgga ttgattccta
     3121 atgcagtatt aggtgccatt ttaaaatatt ttagtgcgta tccctttgcg gtaacgatcg
     3181 cacagttagc agttattttt caactagcaa cacctttgat cattggtggt ttgattgcct
     3241 tacaatttgg atttgatccg atgagaatga tggttgtagc cggagcttct tttgttggtt
     3301 ctggtgtagt caaattcaca cctgatttgg gtgaaacggg tgtttacgtt ggtgctggta
     3361 caggcgatat tattaatacg atgctgacag catcgattgc tgtcgggctg attttactga
     3421 ttggaaatag ttttgggtca gtgtcgattg tgttaacacc gattgttgta ggtgtaggtg
     3481 caggtttact tggtttttat ttattccctt ttgtaacagc aattacttct gcaattggat
     3541 cattgattaa taacttcact accttacaac cgctgttaat gtctatcttg attggttgtt
     3601 cctttgcctt tttgattatc tctcctattt caacagtagc aatcggaatg gcaatccaac
     3661 tagatggtgt ttctgctggt gcggctgcga tgggcgtagc tgcaaccact tttgttttag
     3721 tagtgaactc ttggaaggtt aataagtcag gtgtgacaat tgcgattgct ttaggcgcta
     3781 tgaagatgat gatgcctaat ctatttagaa aacctgtgat tcttattcct tgtttagtta
     3841 gtgcagtgat tacagctatt cctgtcgctc tattttctat ttcaggtacg cctgcttctg
     3901 ctggtttcgg cgtggtcgga cttgttggtc cgttggcttc tttagatgca gggctaaatg
     3961 gctttcttgt tgttctttgt tggttagtca tccctgtttt tgcggcacta attactcaat
     4021 ttttatgcga gaaagtattt aaactgtatg atcgagaaga agtcttcaaa tttttaggct
     4081 agtagatttt actaagaaat tttcaatcaa tggatgcgta agtgagatgt caacaacatc
     4141 tgacttacgt ttttttgcct ttttgagtag gagattgatg ctgacaactg aactcttcag
     4201 tgccattcga ttttttaaaa tgtagaagaa actcaccttt atgttattga ttgttcaaaa
     4261 ggcaactaga ataaaaatat gtgttaaaat gagaaaaaat ttgattgttg gatgtatcga
     4321 ataatcacta aaaagtaggg gaagaaaatg atttttttgc aaaaatatgg tttttatttt
     4381 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
     4441 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
     4501 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
     4561 ttgtatcacc tatttgttgg aacatcaaaa acacttgcaa tacttgttgt tgcaacagtt
     4621 ggtttatatg ggataggtga ttgtattttt actggcttat tcagtatcaa tacgaatgaa
     4681 agctcttgga atctatcgac ttggatccat aatataggtt ctgggctagg ctatgcaggc
     4741 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaagtggtca aggtacgctt
     4801 agccattttt atcttgtttt aacagttatt agtttactaa ttgcaggtct ttacggatta
     4861 gcacggattc cgtcgattag tcagtttgcc ttctttaagc aattgggctt ctttcaacga
     4921 ttaagttttt tctttaacta tttgccgatg atttgtttaa gtttttttca actacgaaag
     4981 tagaaaaagc agtgtgaagc tgattaagtg gttctatgat atttggtgtt gttactcaag
     5041 gcgagtaagg acaccaaata tcatagaacc cttctttttt tgtatagata aactgcttct
     5101 atcactgatc atcgatgttg tctaactgat gtcgccgaat gcaagcatta cctttgtcag
     5161 attaaaaaat actcgttaca cttaatatag aacgacgaaa gaaaggatga tctcaatgaa
     5221 aaaaatgtac gaaacagcaa tgatcaatcg tggaggccgt gatggcgaag ttgaggcacc
     5281 aaacggaagt atgcacatga agatcgaccg cccaggaatc catagtgaag ggacaaatcc
     5341 ggaacaactt tttgcggctg gatacgcatc ttgcttcaac ggcgcggttc aacacatgct
     5401 ggaagaaaat aatcttgagt ctgattctga agtcaaagca cgggtatctt tatttcaatt
     5461 agaagatggg ggctaccaaa tcggggtcgt tttagaagtt tctttgccag gacttgagaa
     5521 ggaccaagca gagaaaatcg cagaagaagc ccacgcattt tgcccatact ctaaagcaac
     5581 aagaggcaat attgatgtgg aagtgactat agtagaataa gggattctta atgttactat
     5641 aaatgagatt atgaaagatc ctataaagaa acgctagtaa aagaaacatg tagttacggt
     5701 aattaatagc ggtatatgga cgattgatcg aatagaacaa ggaggcaaaa aaatgacaca
     5761 gcgggtagca ataatgattt tagtgttgct ggtcattggc ctccttgttt actatgtcct
     5821 aaagtttaag cattggaagc agcaacggat acatcaggat attgaaaaaa agttaaaacg
     5881 gtatcctatt gttcaagcag cttgggaaaa ggctgaagca aaacaatata acattcctgg
     5941 attaacagaa acaagaatgg tggtgccgga aaccggcgaa aatgaggttt gtcaatggat
     6001 gacgccgcaa gggttggctt tttctcaaga ttttgtgttt atttctgcct actgctatga
     6061 ccatcagcat cattctatta ttcatgtact ggatagagaa acaggacagc cgatcaaact
     6121 gctgatttta cctaaacggc cacatgtagg gggcttagta tatgatacaa aacgggaact
     6181 tttatggttg acaatcacag gatctgctac tggacgagta gccgctttac gtttgatcga
     6241 tatcttagca gatacatctg aagagacggg acagccaatc gcttattggc tgacaactga
     6301 tctctcagag attccacaag catcgtattt gactcaaaac aatgatcaac tggtatctgg
     6361 caacttcaca ctaaaaggtg aaggacagtt gactttctat ctattgccga cgatagctga
     6421 aatgaaaaca gcaattcgcc gcaaagacaa aatccaacca accgtgacaa tgagcagaaa
     6481 aacatctgat aagattcaag gtgtggcttt ttatggccat tatctcttag ccagtcgttc
     6541 ttatggtcct tatacctcgg aactcttagt gtccgagcgg gactctccta gccggatcct
     6601 aaaacgaatc aaatttccac cgtatttgga acaaatcgtt gttgtagaag atcgtctagc
     6661 agtattgttt gaaagtggcg ccgcagcgta tcgcgagaaa gccaatcctg tgttagccaa
     6721 tgttttatta ctagacttag caacgttgct tcatgcaaat aagcgtccca aaaagattgc
     6781 tgcaactaaa aaatagtcgg acaactcaag atgatcactg ttgttcataa agaacgacaa
     6841 gaaaaatcat ctagcgatgt tcaactatgg gtttatttag gaagtatttc tactaagatt
     6901 caatatggct atacagattc ggcgaaaaaa aaaggcaatg ttaagttttt aagaatcaca
     6961 gatatacaag agggacgtgt aaattggtcc agtgtgcctt actgtgatat tagtaattct
     7021 aaactagttg atttaagatt ggaagaaaat gacattttga tagcaaggac aggaggaact
     7081 atgggaaaaa gtttcttggt taaggaaata agtgaggaat ctgtttttgc ttcttacttg
     7141 attcgaatta ggttagttga gaaattatta tcagaatacg tagactgctt cttagactcg
     7201 ccattatatt ggaagttatt agaaaaaatt tcttacggaa ctgggcaacc aaatgtaaat
     7261 ggtacaaatt tatctaaact acttatacca ttaccacctt tagaagaaca acaacgcatg
     7321 actactaaaa tagaaatgat aagaagatct attcgtagaa tcaattttga gtaaatataa
     7381 cggacctagc atccatttta taccagatga tgctaggtct attatttata ttttatggaa
     7441 tttaattgat ttgttctagt gttttaatat tattacgaca gtaaaaaaat agtttgattc
     7501 ttctaacatg aataagagag tgtaaaatat tttgtgtaaa tgaaaaaatc catacaaaaa
     7561 aggaag
//
LOCUS       DACCKU010000115         7553 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.118, whole genome shotgun sequence.
ACCESSION   DACCKU010000115 DACCKU010000000
VERSION     DACCKU010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.118"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(571..1917)
                     /gene="gor"
                     /locus_tag="IVN13_002580"
     CDS             complement(571..1917)
                     /gene="gor"
                     /locus_tag="IVN13_002580"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP7013016.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2051..3292)
                     /locus_tag="IVN13_002581"
     CDS             complement(2051..3292)
                     /locus_tag="IVN13_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7013017.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3310..4233)
                     /locus_tag="IVN13_002582"
     CDS             complement(3310..4233)
                     /locus_tag="IVN13_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP7013018.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4454..6946)
                     /locus_tag="IVN13_002583"
     CDS             complement(4454..6946)
                     /locus_tag="IVN13_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP7013019.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6953..7420)
                     /locus_tag="IVN13_002584"
     CDS             complement(6953..7420)
                     /locus_tag="IVN13_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP7013020.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2079 a         1641 c         1213 g         2620 t
ORIGIN      
        1 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
       61 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
      121 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
      181 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
      241 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
      301 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
      361 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
      421 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
      481 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
      541 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
      601 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
      661 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
      721 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
      781 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
      841 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
      901 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
      961 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     1021 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     1081 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     1141 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     1201 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     1261 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     1321 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     1381 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     1441 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     1501 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     1561 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     1621 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     1681 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     1741 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     1801 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     1861 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     1921 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     1981 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     2041 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     2101 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     2161 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     2221 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     2281 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     2341 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     2401 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     2461 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     2521 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     2581 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     2641 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     2701 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     2761 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     2821 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     2881 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     2941 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     3001 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     3061 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     3121 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     3181 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     3241 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     3301 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     3361 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     3421 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     3481 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     3541 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     3601 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     3661 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     3721 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     3781 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     3841 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     3901 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     3961 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     4021 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     4081 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     4141 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     4201 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     4261 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     4321 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     4381 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     4441 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     4501 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     4561 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     4621 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     4681 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     4741 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     4801 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     4861 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     4921 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     4981 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     5041 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     5101 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     5161 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     5221 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     5281 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     5341 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     5401 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     5461 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     5521 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     5581 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     5641 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     5701 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     5761 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     5821 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     5881 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     5941 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     6001 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     6061 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     6121 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     6181 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     6241 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     6301 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     6361 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     6421 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     6481 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     6541 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     6601 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     6661 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     6721 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     6781 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     6841 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     6901 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     6961 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     7021 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     7081 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     7141 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     7201 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     7261 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     7321 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     7381 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     7441 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     7501 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DACCKU010000116         7349 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.119, whole genome shotgun sequence.
ACCESSION   DACCKU010000116 DACCKU010000000
VERSION     DACCKU010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.119"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IVN13_002585"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IVN13_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP7013021.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="IVN13_002586"
     CDS             complement(2886..3191)
                     /locus_tag="IVN13_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013022.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="IVN13_002587"
     CDS             complement(3336..4541)
                     /locus_tag="IVN13_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP7013023.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5235..5621
                     /locus_tag="IVN13_002588"
     CDS             5235..5621
                     /locus_tag="IVN13_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7013024.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5719..5910
                     /locus_tag="IVN13_002589"
     CDS             5719..5910
                     /locus_tag="IVN13_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013025.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6424..6756
                     /locus_tag="IVN13_002590"
     CDS             6424..6756
                     /locus_tag="IVN13_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7013026.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6666..6869
                     /locus_tag="IVN13_002591"
     CDS             6666..6869
                     /locus_tag="IVN13_002591"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7013027.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7053..7208
                     /locus_tag="IVN13_002592"
     CDS             7053..7208
                     /locus_tag="IVN13_002592"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP7013028.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2462 a         1298 c         1046 g         2543 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5101 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5161 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5221 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5281 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5341 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5401 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5461 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata
     5521 aaactgataa agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa
     5581 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5641 tttgtatgct tctttgctcg tcctcctcct tcccaaaaaa ataaatagat tataagttca
     5701 tgggaagaag gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga
     5761 aatgaagaag cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc
     5821 tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta
     5881 gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa
     5941 aaagatagtt tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt
     6001 atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt
     6061 aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa
     6121 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
     6181 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
     6241 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
     6301 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
     6361 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
     6421 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
     6481 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
     6541 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
     6601 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
     6661 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
     6721 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
     6781 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
     6841 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
     6901 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
     6961 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
     7021 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
     7081 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     7141 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     7201 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     7261 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     7321 ctccttgaca atttgaagcg aagaaaata
//
LOCUS       DACCKU010000117         7158 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.120, whole genome shotgun sequence.
ACCESSION   DACCKU010000117 DACCKU010000000
VERSION     DACCKU010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7158
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.120"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..573)
                     /locus_tag="IVN13_002593"
     CDS             complement(<1..573)
                     /locus_tag="IVN13_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7013029.1"
                     /translation="MDQAKSLETACKLLRLSHVLPTLVRKDLDYSFETKEDWLMYLLE
                     KELACREELKTARLLKQAKFPNKRTLEDYEWHDHICLPNATTKDELLSLSFIHQKENA
                     VFVGTPGTGKTHLACGLGQLAAKSGIDTRFWRVSDLVNELEHHWKNNTLEVFKHRFDK
                     VKLIILDEMGYVPFTKEGAELLFQLISDWYE"
     gene            complement(573..1997)
                     /locus_tag="IVN13_002594"
     CDS             complement(573..1997)
                     /locus_tag="IVN13_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP7013030.1"
                     /translation="MLTMTDINTIRNLRNNHDKSINHIAKELDVNWRTAKKYADDDLL
                     PQPKARKKTGMMYTEKWGGIVSLWLSEDAKLPKKKRRTAEAMTTALKADGFPGSYRTV
                     CVFIQDWKATHYLETLEDQGFERLEHPPAEAQLDFGTMEVCHDGAFKDVKVLVMTFPF
                     SNAGFAVVLPAENQECLLEGMKELFSQSGGVPRKIRIDNMSTAVTQTKTKTEQAVLTD
                     GFLRFSMHYGFETQVCNPRSGNEKGSMENKVGYVRYNFFSSTPIMKDFASFNEALAVE
                     LSHDRERIHYEKHVIINDLWNTEKTDLLALPDTDYPVFKEIEVKVNKYNEIKLDNERI
                     HVPRVRNYSIIYGLLRFDSYQLISTDGEIISEGLRPYMMKKRAIDWQAILPDWRRKSR
                     AMHYSRYWKYLPERIKLFLSHPDWKEQEKRLNQISGLLVTHDMLGIDTHFYDMFSAST
                     TTDAYDIDWHQYDAFLSLTGEVID"
     gene            complement(2392..3234)
                     /locus_tag="IVN13_002595"
     CDS             complement(2392..3234)
                     /locus_tag="IVN13_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7013031.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            3559..3975
                     /locus_tag="IVN13_002596"
     CDS             3559..3975
                     /locus_tag="IVN13_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7013032.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIRESQQSVIDCRKLLVEQLTGGDDL"
     gene            3972..4442
                     /locus_tag="IVN13_002597"
     CDS             3972..4442
                     /locus_tag="IVN13_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7013033.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            4456..5229
                     /locus_tag="IVN13_002598"
     CDS             4456..5229
                     /locus_tag="IVN13_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7013034.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            5243..6055
                     /locus_tag="IVN13_002599"
     CDS             5243..6055
                     /locus_tag="IVN13_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7013035.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            6109..7074
                     /locus_tag="IVN13_002600"
     CDS             6109..7074
                     /locus_tag="IVN13_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7013036.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
BASE COUNT         2236 a         1246 c         1387 g         2289 t
ORIGIN      
        1 ttcataccaa tcgctgatca attggaaaag cagttccgcg ccttctttag tgaaaggcac
       61 ataacccatc tcatcaagaa tgattaattt tactttatcg aagcgatgct tgaagacctc
      121 taaggtattg tttttccaat ggtgttccaa ttcgttgact aaatctgata cccgccaaaa
      181 gcgtgtatcg atacctgatt tggctgccag ctgacccaat ccacaagcta gatgtgtttt
      241 accggtaccg ggtgtaccca caaatacagc attttctttc tgatgaataa acgatagact
      301 caataattca tctttagtcg tcgcatttgg caaacatata tgatcatgcc attcgtaatc
      361 ctctaaggta cgtttatttg ggaactttgc ttgttttagg agtcgggcag ttttcaattc
      421 ttcacggcaa gccaactcct tttccagaag atacatcaac caatcttctt tggtttcaaa
      481 gctgtaatct aagtctttgc gaaccaaagt gggaagaaca tgggaaagtc tcagcaactt
      541 acaggcagtt tctaaacttt ttgcttgatc cactagtcaa tgacctcccc tgttagagac
      601 aagaaagcat cgtattggtg ccaatcaata tcatacgcat cggtagtagt cgaggcacta
      661 aacatgtcgt aaaaatgtgt atcaatccct aacatatcat gtgtcaccaa taaacctgag
      721 atttgattta aacgcttctc ctgttctttc caatctggat gactgagaaa taacttgatt
      781 cgttcgggca aatacttcca atagcgagag taatgcatgg cacgcgattt tctgcgccaa
      841 tcaggaagaa ttgcttgcca atcgattgct cgttttttca tcatataagg acgaagtcct
      901 tctgaaataa tctcaccatc agtgctgatt agctgatagg aatcaaaacg aagcagccca
      961 taaataattg aataattgcg tacacgtgga acgtggatcc gttcgttatc taatttaatt
     1021 tcgttgtact tattgacttt gacttcgatt tctttaaata ctgggtaatc ggtgtccggc
     1081 aaagctagca aatctgtttt ttctgtgttc catagatcat tgataatgac atgtttttcg
     1141 taatggattc gctctcgatc gtgtgacagc tccaccgcta atgcctcgtt gaaactggcg
     1201 aaatccttca taatcggtgt agatgaaaag aagttgtacc gtacatagcc gactttgttt
     1261 tccatactac ctttctcgtt tccgctacgt ggattgcaca cctgtgtctc aaaaccatag
     1321 tgcatagaaa atcggaggaa accgtcagtc agcaccgctt gttcagtctt tgtttttgtc
     1381 tgagtaacgg ccgttgacat attatcaatc cgtattttcc gaggaacgcc acctgattgg
     1441 ctgaagagtt ctttcatgcc ttccaacaaa cattcttgat tctcggcggg gagtacaacg
     1501 gcgaatcctg cattactaaa agggaaagtc atcactaata ccttcacatc tttaaacgct
     1561 ccatcgtgac aaacttccat cgtaccaaaa tcaagttgcg cttctgctgg cgggtgttca
     1621 agacgttcaa agccttggtc ttccagtgtt tccaagtagt gtgtcgcttt ccaatcttga
     1681 atgaatacac agactgttcg gtaggatcct ggaaagccat cggcttttaa agcagtagtc
     1741 attgcttcag cagtcctacg ctttttcttt ggcagcttag catcttctga aagccatagg
     1801 gaaacgatgc ctccccactt ttctgtatac atcataccag tctttttgcg tgctttaggt
     1861 tgcggcagca aatcatcatc tgcatatttt ttagccgtgc gccaatttac atctagctcc
     1921 tttgcaatat gattaatgga tttatcatga ttatttcgta aatttctgat agtattaata
     1981 tcggtcattg tgagcatacc tttcttacct ccagttacag tgtcgagact ataactttag
     2041 aggaatttca ggttgcccgc aatggctttt tatcttaact taatcgattt gcggagttgt
     2101 gtagtttttc gttgcacttg tatgtacttt tattctgcca taaacagctt aatgaatgtc
     2161 cacttgaaaa agtgaataaa cgaaatagat gactcgattt aataaggaag taaacccaca
     2221 cagaaatata ggtgattttc cttgaatgac caagatattg aaagttagtg aaaaaacaca
     2281 aaaaggtagc cctattcaca gaaggtctgc cttttgtgtt atcgccagca aacattagag
     2341 gggatatttc atatagcttt tgtcctctcg acagtcctat cagtttctct tttaacttat
     2401 ctctaagtgc tgtccagttt taatagcttc tagcttgttt cctaactgtg tttttaatat
     2461 gttgaaggct tttgaacctg tgcagtggca agtaaggatt ttttctactc gtgagtcatt
     2521 caatttattt ccaattttca ttacctcttg ctcaggtaga cctaaattat ttatataagg
     2581 tatgccaatc atatggaagc caccgattag gtattttatg ggtttagtcg gaaatgtttc
     2641 aagagcaatt tccatcatat cgataatgcc atgatgacta cagccagtaa agacaatcag
     2701 accgtcttct tgctcaatta cgaccaataa ttcatctgaa aaatcatcat aaacaaattt
     2761 atcatccatc tttttgtata aattcttggt ggtcgagttt gctttgccta gggggatgat
     2821 atggaagctc ggagcaagtt ctgttttcgt ttttattgta tgtaaattct catgcatttt
     2881 aaggatttca tttttaggaa taccaacata cagaaacgtg gttaaaatct ttaaataatg
     2941 gggtaaagtt acaccttcag ctgtataaat aggggcgttt cctgatttta aatatcttaa
     3001 cccgccggtg tggtcagaat gaccgtgtga aataatcact gcctccagat cattcaaagt
     3061 ttcccccatt ttagtcgcat tatagactaa ttttttatta gcgccagcat cgaagagata
     3121 tttcttccct gagtagttaa ttaagagaga taacccatgt ccacttttaa agttcaaatt
     3181 tattcttgag ttttcaaata aaaccttgat ttccactata acatctcctt tcacatgaac
     3241 gtgtattcat atattgtagc acattaagtt ctattttttg ttgtaattac cgaaaaaaat
     3301 attaatggac gctttttacg ctttatttcg tatgtggaag aaaatttctt aggatgtggc
     3361 gggggattgg ggcaaagcta aattccaatt acagtgatca tttaattaga cacctttaaa
     3421 tacccacact ttcatcaaca ctataattta gggaaagaaa agtgttggca tgatatagaa
     3481 aaacctttaa ttgacagcgt ttcctaaaat tggcacgcat cttgcttata gaaaagcgta
     3541 aggataggag gaatgaaaat ggcaactata attcttgcca gccatgggga atttgctcaa
     3601 ggattaaagc aaacagcgac aatgattatt ggagatagtg taccaattca tgccttgtca
     3661 gcatttagag atgaagatga atctattttg gtacaaataa aaaaactttt agccacaata
     3721 gatatagaag aaacctacat tttaactgat attctaggtg gtagtgtgaa taatgatatg
     3781 ctcactatta ttaaagagta tgagggattg caattaatta ctggaatgaa cttaccttta
     3841 gttatttcta ttgcaaccta taatggaaaa attggggaaa gtgagctaga aaagcttatt
     3901 agagagagtc aacaatcagt gattgattgt agaaaattat tagtagaaca acttactgga
     3961 ggagatgacc tatgattaaa ttagtacgta ttgaccatcg tttgttgcac ggacaggtag
     4021 tattttcttg gagtaaatca ttaaatatca atcgtattat tattgccaat aaagaggcag
     4081 cgaatgatga attcaaaaaa atgtctttga atttatctaa accgactggc attaaattaa
     4141 caattttttc agttgaacag ttactagaaa aaatgcctaa aatcgaaaca cttaacgaaa
     4201 atatcatgct tatttttggt aatacaaaag aaattcgtca attttgtgaa gcctatccta
     4261 aagtacaaga aatcaactat ggagggataa tcaaaaaaga aggttccaag caatttagta
     4321 atgccatttt cttgaatgaa tctgagcaag aagatgccaa aaaattaaaa gctcttggca
     4381 ttaaacaatt tattcaacag gtaccaactt ctaaaaaaga agatttgaat acaatgttat
     4441 aaaggaggaa tgaaaatgct cgttcagtca attggtttag ggttaatcgg tgttttttgt
     4501 attttagact caaggctttt aggaagaatg aattttgaac gaccgttgat tgtcagtacg
     4561 cttgttggtt tggtacttgg agacatggaa aaaggattaa ttgttggagc ctctttggaa
     4621 ttgatgtcct taggattagt aaatatcgga gctgctgcac cacctgatat gaatatggca
     4681 tcaattattg ctacagcctt tgccatttta tcaaatgcga acgcagaagc agcattaacg
     4741 attgccattc caatttcagt gttaggtcaa atactaggta tcttaatgcg gacgattttg
     4801 tcaaatttga ctcacgttgc ggatcgcttt atcgaacaag ggaaatttag ccaagcaagg
     4861 agaattcata tactttatgg ctcgggatta tatgctctca tgtattttat tcctattttt
     4921 ctagcaattt attttggaac gaatcttgtt tctcagattg ttgatgcgat tcctgagtgg
     4981 ctaacaaatg gcttaacggt tgcaagtaaa attttaccag catatgggtt tgcgttgtta
     5041 ttacaaacga tgttaacgaa aaaaatggtg ccatttttat tacttggatt tttaattact
     5101 gcttattctg gattgggaat cattggtatt gcagccttcg catgtattgt tgcgtttgtc
     5161 atggttcaag ttcaaggtaa aaactcaaat ggtaaccaaa atgagccaga tccattagat
     5221 gacttataag gaggtagatt aagtgaaaga gaaagatcaa aatgcatgga aatatcggca
     5281 gttcttatgg cgttcatggg caattcaagc ctcatggaac tatgaacgac aaatgaacat
     5341 gggttttatg tacggaattg caccaattct agataacatt tacaagaatc cagaagatga
     5401 agcaatgaag aaagaagctt acgagcggca tatgatgtac tacaactgta cccctcaaac
     5461 aagtgcattt gtgttagggt taactgcttc tatggaggag caatactacg aagaccaaga
     5521 aaactttaat ccggaatcaa tcagtgcagt gaagacttct ctgatgggac cgctatctgg
     5581 tgttggtgat tctttctttc aaggaacgat tcgggtatta gcctttggac taggaattaa
     5641 tttagcccaa caaggtagta ttttaggtcc aattctagca atgttaattt cctttgtacc
     5701 ttccttttta gttacttact atggtgggaa aattggctat aacatgggga ataaatactt
     5761 gtctaaactg tatcaagaag gtttgatgga taaagtaatg tatgtttgct ccattgtggg
     5821 gttgatggtc attggttcga tgattgccag catgatcggg attacaacac caattcaaat
     5881 gggtaaagct tttgtcttac aagatgtatt agatagtatt atgccacaat ttttaccatt
     5941 aggattaacc ttcttcatgt actggttatt gcagaaaaag gtcaaaacag gatggatgtt
     6001 ggcaatctgt ttagtcggag gaattatttt tagtttgtta ggtatcttac aataagggaa
     6061 taaccaataa atttggttga ataatataaa taaggaggaa ttcaattgat gaattcaaaa
     6121 gaaattgtta aagaaatcgt agcgaaaaaa gaggtaaaat caatcgtatt tgttggctgt
     6181 ggcgcttcaa aagcggacct ttatcctgca aagtattttc tagaccaaaa tgcgaagcag
     6241 ttacggatta gtcattacac agccagcgaa tttaactatg caacaccagc atctgtagat
     6301 gagtcaactg tcgttatttc agcttcttta ggaggtacga ctccagaaac agttgaagct
     6361 aacgcagtag ctaaagaaaa aggtgctaca gttattagtc taacacgaag cattgactca
     6421 ccgttaacaa aagatgcaga ttatgtaatt tatcatcgat ttgcagagaa ctatgctgct
     6481 aaattagaaa aaacaggcta ctgtttagaa ttggcagtag aacttttaca tcaagtagaa
     6541 ggttatgacc attatgaagc aatgcaagaa ggatttgcta ctatctatga tcttgcccaa
     6601 aaaagtgcag aatcagctcg taaagatgcg aagaaatttg ctgaacgctt taaagatgaa
     6661 aaagttattt atgtaatgag tagtggtgct acacatgaag tagcatactc aacatcaatt
     6721 tgcttgatga tggaaatgca atggattaat tctggtacct tccattcagg agaatttttc
     6781 cacggaccat tcgaaattgt ggataaagac gtaccgttta tattattaat gaatgatgga
     6841 aaaactagaa atatagatgc tcgagcatta actttccttg aacgctttga tacgaaaaca
     6901 gaagttgtag atgccttgga ttatggcttg tcttcacata ttgctaaaga agtcttagac
     6961 tactttaacc cattcgtgat tactgcagta tttagagttt atgcagaaga attgtcagaa
     7021 gcaagagaac atccattaac taagagacgt tatatgtgga aactaactta ttaaaaaata
     7081 gatcaaacta aataaggtgg cttgagaaat taagccacct tgttttcttc aatgtgattt
     7141 tatacatatg gtaaaata
//
LOCUS       DACCKU010000118         7083 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.121, whole genome shotgun sequence.
ACCESSION   DACCKU010000118 DACCKU010000000
VERSION     DACCKU010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.121"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IVN13_002601"
     CDS             <1..75
                     /locus_tag="IVN13_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP7013037.1"
                     /translation="PETAIRMEIDQLNRQQRLFGDENE"
     gene            251..592
                     /locus_tag="IVN13_002602"
     CDS             251..592
                     /locus_tag="IVN13_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013038.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLWKQPRKPEYIYVIEDGLIDALEEYDRDNLIDISYYGQGKK
                     YIAEMEAEFDE"
     gene            585..1331
                     /locus_tag="IVN13_002603"
     CDS             585..1331
                     /locus_tag="IVN13_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP7013039.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPQDVSMLEPASQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            1337..2023
                     /locus_tag="IVN13_002604"
     CDS             1337..2023
                     /locus_tag="IVN13_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013040.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            2027..2773
                     /locus_tag="IVN13_002605"
     CDS             2027..2773
                     /locus_tag="IVN13_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013041.1"
                     /translation="MDHRSYYAIIPANVRYDDSLIPSAKLLYGEITALCNEKGYCWAS
                     NEYFANQYGVRKSTIQNWLKSLEEKGYIYREVKYKEGSREIEARYIRILGGGHPEKWV
                     GGHPEIYQDNNTSINNTFNNTKEYIRELPPSKKSKAKPIRHKYGEYKNVLLSDEQMEK
                     LKIEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGRR
                     EKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            2785..3054
                     /locus_tag="IVN13_002606"
     CDS             2785..3054
                     /locus_tag="IVN13_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013042.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDGTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            3217..3522
                     /locus_tag="IVN13_002607"
     CDS             3217..3522
                     /locus_tag="IVN13_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013043.1"
                     /translation="MDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKTKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSSWIDSKGRQFRYVEEKKC"
     gene            3516..3806
                     /locus_tag="IVN13_002608"
     CDS             3516..3806
                     /locus_tag="IVN13_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013044.1"
                     /translation="MLDMRIEDYRITSDSRNIVLSKVRRDEEGNIRYTEAKEESRADI
                     GYFQTVSSCLKAIQRDYVLSEERTIKSIIEYKKALENITRQFEQACEIEEEK"
     gene            3806..4123
                     /locus_tag="IVN13_002609"
     CDS             3806..4123
                     /locus_tag="IVN13_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013045.1"
                     /translation="MEFVDLNVRGIKCDNPECDYSDMAVKYENYPQWLNKPCPKCGAN
                     LLTQEDLDATEQLMEIVNLTNEILKDSGLEKQDMNKYIVPVEANGTGELSFGEIKKLE
                     EEK"
     gene            4120..4935
                     /locus_tag="IVN13_002610"
     CDS             4120..4935
                     /locus_tag="IVN13_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HAP7013046.1"
                     /translation="MNENKLIKLGVAGVVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKEHGWVTVNGADTVVTDETGK"
     gene            5074..5388
                     /locus_tag="IVN13_002611"
     CDS             5074..5388
                     /locus_tag="IVN13_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP7013047.1"
                     /translation="MDLITQYSEIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            5565..5876
                     /locus_tag="IVN13_002612"
     CDS             5565..5876
                     /locus_tag="IVN13_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013048.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEEA
                     E"
     gene            5873..6076
                     /locus_tag="IVN13_002613"
     CDS             5873..6076
                     /locus_tag="IVN13_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HAP7013049.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGISAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            6073..6369
                     /locus_tag="IVN13_002614"
     CDS             6073..6369
                     /locus_tag="IVN13_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013050.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EEFIYPFRGYVEHVYNHSAIIRIENTMECDKWTAKSKENLAVVRLVDMELINDK"
     gene            6446..6862
                     /locus_tag="IVN13_002615"
     CDS             6446..6862
                     /locus_tag="IVN13_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP7013051.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDALIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2646 a         1088 c         1600 g         1749 t
ORIGIN      
        1 ccagaaactg cgatccgcat ggaaatcgat caactgaaca gacaacaaag acttttcggt
       61 gatgaaaatg aatagagctg aagcgctaaa aataggcgta ataattgcta atcgctggtg
      121 gagacacaat aaaccaaaca tcctaagcca acaacatatt gataagcaaa aagcatggca
      181 acaaataaaa agcgactccg ccggcaagca ttgagtcgca aacaaaatac atctaaggag
      241 atgttaccac atgaaaaaag aactttccac tctagatcaa tatttgactg atcctagttg
      301 gggcaaatcg aatatcaagg aaacaagcaa tcgaaaaatc agacgtaatc ttttgacgga
      361 tgaagaacta gcatgtgatc aagacgattt gggaaatttt gtgagtattt ggggacatgt
      421 ctaccttatc catctatgga agcagccaag aaaacctgaa tacatctatg tcatcgaaga
      481 tggcttgatt gatgcactag aagagtacga cagagataac ttgattgata tctcttatta
      541 cggacaaggc aaaaaataca tcgctgaaat ggaggcagaa tttgatgagt gaaaaggaac
      601 aacctttgaa aaatagaagt gataatacgc tttttaatac cttatacaaa ataaatgtga
      661 aagatgttac tgaaaaacga aataacctca cttatctttc gtgggcatgg gcttgggcag
      721 aagttagcaa agtgtgtgag gcagtagatt atgaaatcta tcatgatcca gaaacatatc
      781 tgccgtatgt ctttgataag aaaacagggt acatggtttt taccagtatc acagtcaacg
      841 gagtaaagcg tgacatgtgg ttaccagtca tggatggtgc aaacaaggca atgaaagatg
      901 agccatatac ctacgaagtc aatgattatc agtggaataa cgaaacgaag aaaaaagaga
      961 ttgttggaaa aatcgaaaag cgagttgaag cagcaactat gtttgatatc aacaaaacga
     1021 tcatgcgctg tcttgtaaaa aatctagcaa tgtttgggct agggctatat atatttgctg
     1081 gcgaagatat gccacaagat gtctcgatgc ttgaaccagc tagccaaaga agcaaaaagc
     1141 tattcttaga tgctttacaa ctggttgcta acaagtacga aaaatcgatt gatgaagcaa
     1201 ttgttgcatt gactgatgca gcttccataa ccgccgatga cagtaaatgg accaagagag
     1261 acttgggcat tctaaaacga ggcgttaact ggattgaaga tcagtacaga gaagaaacaa
     1321 aagagaagtg atatgagtgt ttaaaccatt aatcgattca tattcagcgg ttctgaaaaa
     1381 gttcaaagga aaagacatag gtgcaacgat caatgaagaa gtgaacattg atcgactaaa
     1441 gacgatgtat gacggctacg atggcgatcg agtcattgaa attcgtttta ttgatcctag
     1501 acgtttcacc gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga
     1561 tacaggcatg ccaacggact tctggaagga attcttctac ttccgttttg aaggcgtcac
     1621 agggcgcaaa ataagcctca aagacgaatc gaatacgact gtgagtgatg ctaatgtctt
     1681 agcaaatatc attttagatt tcatctttga acaccatatt cctttcaaag aaggctatga
     1741 gattttacca gcgaatcaag agtattactt ctacaaatgc atcacaaaaa gagtctgctg
     1801 catctgtggc aaaacaggag ctgatattga tcactttgac aaagctttag gaagacgaaa
     1861 gcgcaaagaa gttgatcatt cagagtacac atttgcagca ctctgcagaa tccatcacac
     1921 agagaagcac aagataggtg tgatcaattt caaaaataag tatcaaatca aagggatcaa
     1981 gttaaaccag gaaacaatca agaaattaag aatcggagga tgaaaagtgg accacagaag
     2041 ttattacgct atcatacctg caaatgttag gtatgacgat tctttgatac ctagtgcaaa
     2101 acttctttat ggagaaatca cagctctatg taatgagaaa ggttattgct gggctagcaa
     2161 tgagtacttt gccaatcagt acggtgtaag gaaatcaacg attcaaaatt ggctgaaatc
     2221 tcttgaagaa aaaggctata tctatcgaga agtgaagtac aaagagggta gtagagaaat
     2281 cgaggctagg tatatcagaa ttttgggtgg gggtcaccca gaaaaatggg tggggggtca
     2341 cccagaaatc tatcaagata ataatacatc tattaataat acatttaata atacaaaaga
     2401 atatataaga gagttaccgc cttcgaaaaa atcgaaggct aagcccatcc gtcacaaata
     2461 cggagagtat aaaaatgttc ttttgtcaga tgagcagatg gagaaactca aaatagaatt
     2521 ccctaatgac tatcaagaac gaatagaacg gctatctgag tattgtgaat catctggtaa
     2581 gacttataaa aactatttgg caactattcg aagttgggca aggaaagaaa aaagtgaacc
     2641 taagaacgca agcagtggat acaagcgcac aggaagacga gagaagcttc ctgaatgggc
     2701 aatcgaccaa gaagcctatc ttaagaaaaa agcgctagaa cgagctaata gacaatcaaa
     2761 agcaccattc taagaggtgg aaaattgaag atcgattatc tagaactaat taatgaaata
     2821 gcaaagtata aaactggtga ggaaatagaa attctgagag acgtatatga tcaactcgaa
     2881 gaagctggaa ttgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat
     2941 ttcgcacttt atatcgatgg aacacaatta agaaatttag catataccaa attactattt
     3001 gttgattgtg ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaaacaaga
     3061 tggacctaaa gacatttaca gcacagatcg aattaatgca tcaagaagct ttaagacaaa
     3121 gtgtatcgta cgaagacaag tggctcaaca cgtttcatgg cggacgtgag agcgcacttg
     3181 atcaagtact caaattattg aaaggagaat gtggggatgg ataaaaaagc ggcaatgaaa
     3241 cgaattgctg aattaaccaa gtcagaatct tggcaagaag acaaagaaat agttgcagaa
     3301 gtccaaaaac tcggcaaatc aatgtggact gaaaaaacca aacggaaaac gccgagaaag
     3361 attgcaatct ggcatggtga tcgaattcta gtaacaggta ctgctgaaca gttatctgaa
     3421 attactggat taagcaaaaa cattatttgg gatagagcta ggagctcatg gattgattca
     3481 aaaggacgac agtttaggta tgtggaggag aaaaaatgct agacatgaga atcgaagatt
     3541 atcgaattac cagtgattct agaaacattg tcctatcaaa ggtaagacga gatgaggaag
     3601 gaaatatccg ctacacagaa gcaaaagaag aatcacgagc agatatcgga tacttccaaa
     3661 ctgtctcatc gtgtttgaag gcgatacaac gcgattacgt gttaagtgaa gaaagaacga
     3721 taaaaagtat tatcgagtac aaaaaagcgt tagaaaacat cactagacag tttgaacagg
     3781 catgtgagat tgaggaggag aaataatgga attcgtagac ttaaatgtac gtgggatcaa
     3841 atgcgataat ccagaatgtg attattctga tatggcagta aaatacgaaa attatccgca
     3901 atggttgaac aagccatgtc cgaaatgcgg cgctaatctt ctgacgcaag aagacttgga
     3961 tgcaacagag cagcttatgg agattgtcaa tcttacaaac gagatcctca aagattcagg
     4021 attggagaag caggatatga ataaatatat cgtacctgta gaagcgaatg gaaccggtga
     4081 attgtcattt ggagaaataa aaaaactgga ggaagaaaaa tgaacgaaaa taaattaatc
     4141 aaactaggtg tggcaggagt agtaatagta ggtattggag ttatcggagg atttaagttt
     4201 ttcgaaaaaa ttgataacgg atatgtgggt gtgcgctatt caatgaatgg cggtatcaaa
     4261 gatgaagcac tgacgcaagg tgtgaaattt gttgggattg acaaagtgat ccaatatcca
     4321 attcgcttgc aaactatcca atcaaaaaac atttcagtat caacaagcga tggcaaaaag
     4381 acaacgattg atatcaaata tgactacaaa gttgattcaa ctaaagcagc aaaaatgtac
     4441 aaagaatttg ggaatataac ttcggaagat atcgaaagtg gatggttaaa atctaagctt
     4501 caaaaagtag cgcgtgaagt ttatgccaaa tatagcctgc ttgatgtcct ttcaggagat
     4561 tcctctaaag ttgaagctga ggtactaacg aactttgcta aatcagttga atctaaaggg
     4621 tttgaagtcg aagacgtaac acttggtgtt ccagatgtcg ataaagaaac acaaaaatca
     4681 attgatgcga tcattcgagc tggtcaagaa aatgaaaaag cgaagctaga tgcagaaact
     4741 gcaaaaactc aagctgatag tgaagcttac aagaaaacaa aagctgcaga agcggaagct
     4801 gaatcaaacc gtaaagttgc cgaatctgta acagataatt taattcgtta tgaagaagct
     4861 caagctcgaa aagagcatgg atgggtaaca gtaaacggag cagatactgt agttacggat
     4921 gaaacaggta aataatatgg gattctttat agctaaaatt ctcttgttct taggtttggt
     4981 aggagtggca tatctcgtgt atgccctcct ttccaaaact gatgacaaag aagatgacaa
     5041 cgacgatgaa atgaaatttt aggaggagaa ataatggatc tcattacaca atacagtgaa
     5101 atcatcctca agaaaatcat gatgaagatt cagaaagata aaaaatcaaa agaacgagcg
     5161 gaattagtta agttggaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg
     5221 aaagcagcag caaacattga attctattac aacgaaattc aaaaagggtt cgatcagatg
     5281 cgtgagctgg atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt
     5341 gtagaaaagt atagagagat actgaatgag tatttcgagg aggactagat gtttaattta
     5401 atgttagcgt caattcactt tttattctat ataacgatat ttgtagctat attaattaat
     5461 acgataaaaa gcggtttttt aacaccaatc ggcacaattt taacattagc ttcatttgtt
     5521 acaagttgta ttatccaact taaatatata aaggagaatg aataatggac gaactaatca
     5581 caaaagtaga gcagtgggct aaagataagg gattggatca agctgattcc agcaagcaaa
     5641 tgttaaaaac gatcgaagag attggggaag ttgccgcttc tctagctaga aaagatgaac
     5701 atggtttaag agacggaatt ggagacgtag tagtaacctt gattatttta gctatgcaaa
     5761 atgatatgga tttgtacgag tgtctgaacc aagcatacaa cgaaatcaaa ggacgcacag
     5821 ggaaaatggt agatggtgta ttcgtgaagt cgagtgattt ggaggaagcg gaatgaacaa
     5881 cagacatcgc agaataacaa aactaagaaa acaggaactg aatgtactaa agacaaagtt
     5941 tgaaaaagaa tatggaattt cagcagaaga aacatataaa gtggcaagtc agtgtgttgc
     6001 tgatgcaagt gatgctattc gtaagtttgg aatttcgata ttaagtgatg atcgtaaatg
     6061 ggaggaaaaa gaatgaaact aaaagacgga ttttacgcta gcagtcacgg tatcggcggt
     6121 ttaatgctag atatgccgac aaagaatcct aaaacacgta agaaaccaaa agtcaaagtc
     6181 ggtgacatgg tccgctgtga agcagaggag ttcatttatc catttcgtgg atatgtagaa
     6241 cacgtctata atcactcagc gatcattcgc attgaaaaca cgatggaatg tgataaatgg
     6301 acagctaaaa gcaaagagaa tttagcagta gtgagattgg tggatatgga actaatcaat
     6361 gacaaataaa aaagccggat cgctccgact aacataataa aacagacaag tttattatat
     6421 cacataaaag gagcagtttg acttgatgca attgttacga gaggtagatt tcaaacagac
     6481 aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt ttggagcgga tggcaggtcg
     6541 ctctttgata gatattaagt cgccgattat tacggatatg ccgaaggcac cgaagcacgg
     6601 caataaggca gaggatgcat tgattcagat gatggatata gaagcggaga gagacgcgat
     6661 tttagcggct ttgatggctc ttagtctgat tagtcgtcag atactctact atagcttctg
     6721 tgacgtaaat aagcactcta attatgaaat aggtcaattg atacgaggat acggagagaa
     6781 gaatgtagag aagctgaaat ctatcgcgct gatcgaattt gctgaagcat acaaaaaagg
     6841 cgtgttagtt cagtatcgtt gattttgtag ggtttttgta gggatagtgt agggtttttg
     6901 agtgttttaa cgtgatatta tgatagtgtc gaaagattag gaaacaggat cgacaaaata
     6961 aaatgtaagg gagggaatct ccctcatcgt tttaaattaa gcttcgatag gcagcaacgg
     7021 aaatattaag aataaggatg tgaatttcaa ctcctcctga ttgttcttat tatctatcat
     7081 ccg
//
LOCUS       DACCKU010000119         6768 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.123, whole genome shotgun sequence.
ACCESSION   DACCKU010000119 DACCKU010000000
VERSION     DACCKU010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.123"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(45..1820)
                     /locus_tag="IVN13_002616"
     CDS             complement(45..1820)
                     /locus_tag="IVN13_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP7013052.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSRRKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(2031..2933)
                     /locus_tag="IVN13_002617"
     CDS             complement(2031..2933)
                     /locus_tag="IVN13_002617"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP7013053.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEGGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQAFDPEVFPELLAEEVTL"
     gene            3212..5557
                     /locus_tag="IVN13_002618"
     CDS             3212..5557
                     /locus_tag="IVN13_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP7013054.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKVIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(5720..5986)
                     /locus_tag="IVN13_002619"
     CDS             complement(5720..5986)
                     /locus_tag="IVN13_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013055.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQIWAEKRLTDQNHQ
                     MTAEEAAQEFSNRHVHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(5979..6302)
                     /locus_tag="IVN13_002620"
     CDS             complement(5979..6302)
                     /locus_tag="IVN13_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013056.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(6314..6520)
                     /locus_tag="IVN13_002621"
     CDS             complement(6314..6520)
                     /locus_tag="IVN13_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013057.1"
                     /translation="MTEQEIIEALASVVATKENLVDSAKEVYLLRINKARRMGEAFDT
                     LVKEIQDKINEIVTRDRELAQQFN"
     gene            complement(6517..>6768)
                     /locus_tag="IVN13_002622"
     CDS             complement(6517..>6768)
                     /locus_tag="IVN13_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7013058.1"
                     /translation="FSYQLQELNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSA
                     KMKSAMEEVIRINQRGIFELEKKWHGKKACGKHVLSPLR"
BASE COUNT         2046 a         1360 c         1273 g         2089 t
ORIGIN      
        1 tcggaaaaag gaactgcgac aagacttttg tcacagttcc ctttttattt agaagataaa
       61 gcttttttca gattcaattt actgaaaact agcatgcttg ttactacgat gaattgtgtt
      121 aatggcacgc ctgcgccagt gtagacactt aaaaaataat cctttttttc tggcgcataa
      181 gaacaaaacc acgagacacc gaaaagaaaa accaattgtg tcagtattaa tccggtgact
      241 acaatcaaag aggaactata ataggcacga tcagcttttg caaatacggt tcctagtatc
      301 ataagtaata tccctgctat tgaagcaatc agcacgggaa tggccagaac ttggtaaatt
      361 tttatcatga cattcgcgac ttttgctgtt tttgtcaact ggaaatcagg tgttctcgga
      421 tcactgctat cgatccaatt atccattagt ggaagattca ataattcttg ggcctgttcg
      481 acttgttctt gcgtcccgaa actataattc cctccttgat ggaaatcttt gtatcgccat
      541 gcataggcat atcctgattt cagaaaagca aatacgattg gcagatcatt catcgtcttt
      601 ccatcccctg atcccatcag atagaattct tttttctttt tgatagttcc cttttgatag
      661 gcttcttcta attcttgatt gacttttttc caaaaatcat tggtttcttt tgcattgttt
      721 tggtaataac cagattcgct taccacatct cgcaatgccc aaaaaatgat atctcctggt
      781 aattctttta cttttccgcc ttgggtccac gcatgagtcg tatacaattc gtcaatacga
      841 gtagaaacag tatgaaatgt aggagatgcg tctaatgctt tatttaatgc agcatgcgag
      901 acccatatct cactgttttc tttatttaag ttcgtttcgt catctattcg gatcagctgt
      961 ttcatcaaac gtccaaattc accactcgaa cggtcattga tggtggcaat accatatgtt
     1021 gtctcgtttt tatattcgat ccactgatag acgatggaga aaccgactag agggagtaaa
     1081 aacaatgaaa gtttcagcca ttcttgtttc gaaaggagaa atggtcgttc ttttaacttc
     1141 tttgcttctt tgaaagaaaa gtcttgtcct agcaagatct gaacgaaact gatgcctaac
     1201 gcgactacag taaaaggaag aagccagatg gaatcttcgc gaatgaacca aaagaaagga
     1261 aaagaaacag ctaaccctaa aaaccacggc agcaattttc tgcgggaact atatttacgg
     1321 taataaatag ctagtaaaca agcgataatg atctcaacag ccgggatcac gagagcatta
     1381 cgatacgttc gagtggaata ctcatgtgta aaactaatcg gagaaaacaa aaaataaaaa
     1441 tagatactta aaagaagata cttgttcttc acgatcggtt taaacgctag tgcaaccaat
     1501 atgctggcaa gaatactgaa catgccgaac aaaactgcat aagaaagatg cagtttatgt
     1561 gcaagtacta aaaacaagct gtaggagatc ccttttgaaa gtgtcgtagt cgtgtactcc
     1621 cctagccatt taccatccaa aatattttga gcatagtgaa tcagcagctg atcatcatag
     1681 gttgccgaca tatctgttcc atagattgct ttactatata aatacaaccg aataattgta
     1741 aataatacgg ctatgccaag aataaagtat gtatgtttct tttttagttg gttgatcaag
     1801 ggttttaaag tgttcagcat cttttcgtcc tttcaatcac gttttgctac ttgcttgtga
     1861 atcttttcgt tttttacttt tctatcagta taccctgttt gtctgaattt aaaaagaggg
     1921 ggcgaaagga gcatggcgaa aacgactgca aaaagcactc ctttgtttct taaacaaaaa
     1981 aggacaaggc agactgccca atcctttttt gttgctgtca gatagaaaac ttaaagtgtg
     2041 acttcttctg ctaataactc aggaaacact tcgggatcga atgcttgcgc tttgaacatg
     2101 gcaatttctt ttccatatgg tgcgtaagct ttctttttat ctttaccgac atatggagtt
     2161 tctaagattt tcgaaatgtt gtcaaacatt ggatcatgga cgattttgtt caatgtgtca
     2221 aatccaattg ttccaaaacc gatattggca tgccgatctt tatgagaacc ttgtggattt
     2281 ttagaatcgt tgatatggag tactttaagc cgatctaatc cgatgacctt atcaaactct
     2341 tccatcacac ctgagaaatc ttcttttata ttatatccgg catcattcgt atgacatgta
     2401 tcaaaagtaa cggataattt ttcattcaat tttactccat cgatgatttg cgctagttcc
     2461 tcaaatgttc gaccgatttc cgtacctttc cctgccatcg tctccaaagc gatctgtgcc
     2521 gtctgttctt ttgtcaaaac ttcattcaaa ccctcaataa ttctagcaat cccaccttct
     2581 gcccctgctc ctacatgagc gcctgggtga agggtgattt gagtagcacc taaagcttcc
     2641 gcacgaacaa tctcttctct cagaaattgt acagcaaatg gaaacatttc tggtttgatc
     2701 gtatttccca aattaatgat atagggcgca tgaacgacga tatttgttag tccgttttct
     2761 gccataaatg cttttccggc atcaatattc atctcttcga tagcttttct tctagtattt
     2821 tgaggagcgc ctgtatagat cataaaggtc gaagatccgt aactggctgc ttcttctgcc
     2881 gcgcctaaca gcattttctt tccgctcatg ctgacgtgtg agcctaataa catggatgga
     2941 ttcctccttg aaaggcaatt gtttcgaaca attcctatat attggtaaga tgatgtacaa
     3001 agaaaatggt ttgtttttct ttgccctgtt ctccatagta actgaaactt tctctaaaga
     3061 aaagcttaag aactctttca taaaaaagat catttcctca cgttttatct cattcgaaaa
     3121 agaaattcct gtcaaaaaca gccttgtctg tttttttgtc ttattatttg ctataattag
     3181 cgaaataaaa ccattagttg aggtgaccct tttgccacgc aaaaatactc gaaaaaaaca
     3241 gaatagaaga aaaaaagaaa aatggtttgt tcctaaggtt atttttagag tgtttcaatc
     3301 attgactgtt tttattactg ttcttctttt gttgttcgca gctctaggta tcggaatagg
     3361 tgctggctac tttgcctatc ttgtcgaaga cacacaattg ccgactaaaa aagaactgca
     3421 gacagaacta ggaaacatta cggaaacgtc aaagattgtt tatgcagata atacagagat
     3481 atcaaaaatc cagacagatc tgatgcggac gactatttca tctgataaga tttcgccttt
     3541 attgaaaacc gcgattatca gtacagaaga tgaatacttc gataaacacc aaggttatgt
     3601 ccctaaagct gttttacgag cattagtttc agaagctact ggcatcggct cgtctggagg
     3661 atccactttg acacagcaat tagtcaaaca gcagatccta accgatgaaa caacattcaa
     3721 acgtaaagca aacgagatcg ttctttctgc acaagtagaa aaatactttt caaaagatga
     3781 gatcattgct acgtatttga atgtctcgcc atttggtcgg aataataagg ggcaaaatat
     3841 tgctggagtc caggaagctg caagaggaat ctttggtgta gatgcaaaag atgtgactct
     3901 gccacaagct gcttatatcg ctggtttgcc tcaaagccct atcacgtata gtccctacac
     3961 gaatacaggc gcgctaaaag aagatctttc tgccggtctt gccagaaaag attttgtttt
     4021 attcagtatg taccgtgaag gacaaattac gaaagaacaa tacgaagaag cgaaagccta
     4081 tgatcttaca aaagactttc tcccacaaca aatcgcggaa cagactgaca gagaattctt
     4141 gtattatacc gtgatgaacg aagcaacaag gattattgca caacaattgg cagaaaaaga
     4201 taatgctgat atgtcagatt catcggtcag tgaagcttat tatcaaaaag cacagcaaac
     4261 gatccaaaac aaaggatata cgatccattc gacgatcgat aaagatatct atgctgcatt
     4321 gcaaaccggc gtagaaaatt acggctatat gttagatgat ggtgctggta gccaagtgga
     4381 gactggaaat gtattgatgg acaataggac tggacgtatc tatggattcg tgggtggccg
     4441 gaattacagc cagaatcaaa ataaccacgc ctttgataca gaacgtcaag ctggttcatc
     4501 catcaaaccg gttcttgttt acggtcctgc aatcgatatg ggacttgtcg gatcagaatc
     4561 ccgtgtatca gattatgcaa cgacttggca agaaggagaa aatgctggcg agaaaatcgt
     4621 caatgcgacc aatgaagggt cgaacacctt ccaaaccgtc cgtgagtcac tggaatggtc
     4681 caacaatatc ccagcttatc acttatacca agacgtattg aattcgggtg gatcgaaaca
     4741 atatgcttac gagcactact tggcgaagat gaactaccca gcaaatgata attggggcgt
     4801 tgaatctgct cctttaggga cagtagatgt aacgacgctt caacagacaa atggattcca
     4861 ggccttagct aatggtggcg tctacgaaga aggctatatc attgattcca tcacagataa
     4921 tgcaggtaat gtgatttata aacatgaaag taattctgtc cgtatttatt ccgaagcaac
     4981 ggcctcgatc atgaatgaca tgatgcgcag tgtgatcaat gaaaaaatca ctacaccatt
     5041 caaagatgtc atttcaagct taaacggaaa tctaggaaaa gcagattggg tcggaaaaac
     5101 tggttctacc aacgaataca gagattcatg gctgatcgtc tctactccgt ctatcactat
     5161 cagtagctgg gcaggacacg atgacaatac tggaatggac agccaagcaa ggatacgttc
     5221 ggctaattat cttgccaatc taatcaatca agcttaccaa gcaaaccctg agattttcgg
     5281 cactgatgag aaatttgact tatcatctga tgtgatgaag aaaaaagtat ctgggttcac
     5341 aggacaaaca gctggaaaag ttactgtcaa taaaaaatct atccaaacgc caggaaaaac
     5401 agtggaatca ctttgggcaa aaaatggtcc gaagaaaagc gcctttaaat tcggcgttgg
     5461 cggtactgat gaaaactata cagattactg gaacaccgct tcagcttatg ctagagcgaa
     5521 tccagagacc aaagaatcag acgataaaga tgattgatct aatagattaa tcagaattaa
     5581 agggagctgc gacaaaaatc ttgtcacagc tccctttttc cgaataaatg gtgttcaaaa
     5641 gcttgcatct acgatattaa gacaaattta tgtaaaaatg tgaaaagcca ttttcacaaa
     5701 atttttctat ataactttac taacgaaact gttttaattt cttttcttgt ctttggaata
     5761 attcggttag tttttctgca aattgccctt ttactgattc tttcaagtgt acatgtcggt
     5821 tagaaaattc ttgagcagct tcttctgcag tcatttggtg gttttgatct gtcaatcgtt
     5881 tttctgccca aatttgctga gatatcccca taccaactga acgtacattt gatcgatgta
     5941 gatttttctc taattctttt agtaattgca ttaaaaattc attcataaga tgatttctcc
     6001 atagttattt gtctgatttt ttgacgatag gaacgttcta gttcgtcttg ctgctgatag
     6061 agttttcgtc gttctacttg tagggcttgt tgataatgct cctgataatt cgaataactt
     6121 cgtcttgctt gaaacagagg ctcattatct aaagctaagt tctttaatac atgatgcagt
     6181 cgctgctcct cttgctccat caatcgtaat ccttgattct taaagtggat caattcatct
     6241 tcttgagctt ctaattgtct tcgttcactt cgataatctc tttccaattc ttctagtttc
     6301 atctacttct cctttaattg aattgctgtg ctaattctcg atctcgtgtc acgatttcat
     6361 tgattttgtc ttggatctcc ttcaccaatg tatcaaaggc ctcccccata cgtcttgcct
     6421 tattgattcg cagcagatag acttctttcg cagaatcgac taagttctct tttgtggcga
     6481 caacactagc taatgcttct atgatttcct gttcagtcaa cgaaggggcg acaacacgtg
     6541 cttgccgcaa gccttcttgc catgccattt tttctctagc tcaaaaattc ctctttgatt
     6601 gatgcggata acttcttcca tagcgctttt catttttgct gataactttt ccactactaa
     6661 cctagcttgt gtgtcatcta agtaaatttg ttcattaact gtcagccggc cgccacttaa
     6721 tcgtagtttt ttacgtatgt tgttcagttc ttgcaactga taggaaaa
//
LOCUS       DACCKU010000120         6764 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.124, whole genome shotgun sequence.
ACCESSION   DACCKU010000120 DACCKU010000000
VERSION     DACCKU010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.124"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..648
                     /locus_tag="IVN13_002623"
     CDS             <1..648
                     /locus_tag="IVN13_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013059.1"
                     /translation="TSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEK
                     AIPYIATWTKNSQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPA
                     EVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            709..2169
                     /locus_tag="IVN13_002624"
     CDS             709..2169
                     /locus_tag="IVN13_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7013060.1"
                     /translation="MTEKKMSLIDRCKQMDIVDFARNNGLAVANKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPDWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPREYKVI
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEVISLKD
                     ICREVAKELKDCERSNGTYDFDRFYQGKGDINSQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIHQQEIRKQKRAMLFQQGRQQERE"
     gene            2180..4384
                     /gene="topB"
                     /locus_tag="IVN13_002625"
     CDS             2180..4384
                     /gene="topB"
                     /locus_tag="IVN13_002625"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7013061.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            4630..5223
                     /locus_tag="IVN13_002626"
     CDS             4630..5223
                     /locus_tag="IVN13_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7013062.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            5239..6138
                     /locus_tag="IVN13_002627"
     CDS             5239..6138
                     /locus_tag="IVN13_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7013063.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            6141..6626
                     /gene="ssb"
                     /locus_tag="IVN13_002628"
     CDS             6141..6626
                     /gene="ssb"
                     /locus_tag="IVN13_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7013064.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         2498 a         1154 c         1416 g         1696 t
ORIGIN      
        1 acaagtattc acgaattagc ccatgctaga atgcacaaat tttcagaatt agatactgca
       61 acaaaagaac tacaggcaga aatgacctcc tatattgtgt gcaagcattt tgggatggac
      121 acctcggaga aagctattcc ttatattgcg acatggacga agaatagtca gacgttggat
      181 gataaggaag ctgcggaacg tgggaaaatc atgaatgatg tcagtcgtgt cgcaaatgaa
      241 ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccag
      301 ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagaaca aaagaatatt
      361 gtgcccgttg gctcactttg gatcgacaag aaagacggga aagtcctgga agaagacacg
      421 ggccgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg
      481 attttaaaag gaaaaagtta tggaaaagag atccaaacgg cgttaccttc agaagaattt
      541 gcgctaggaa atccattccg attttcactt gagacagtgt cagaagtggc aaaacgaaaa
      601 aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa caagcaagaa
      661 agactatctg aaaaatggat agtctttttt cttttgggag gtttgaaaat gacagagaaa
      721 aaaatgagtc tcattgatcg atgcaagcaa atggatattg tggattttgc acgaaacaat
      781 ggtttagcgg tggccaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt
      841 tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt
      901 gaattagcta aacttttttt catagacaaa gagatccaag accctaagca acaatttaaa
      961 gcagccactg attttattct aaagaatgaa gacaaaacag aacgagtgga aaatcttcat
     1021 tttgagacag aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga
     1081 aggaattatc tgaaggaaga acgaaaactg cccgattggt tgattgatta tgcagaaaaa
     1141 gaaggattga ttgccgaact aaagccaaaa catgaacggc aaaatttctt agttcgagac
     1201 gatcgtttgg atcatgcggt cgccttttta tggaaagatc cacagacaaa agagacagta
     1261 ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag aggcacatat
     1321 aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat tggtgatcct
     1381 aaacagttaa aattttttga gagtagtatt gatttactta gctacgctgc actgaatcgt
     1441 gatcagttaa acgatacgtg gttagtctct atggaagggt taaaacacca tgtgatcagt
     1501 cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatccatc
     1561 gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga acaattaatg
     1621 ggcgcagttg atccatttac gaatcagaaa gtacgttgtg aacgaggaat cgctaatgat
     1681 tggcaagtgc caagagaata caaagtcatt tatgaagaag tcgcaaaaga agaaaaggtc
     1741 acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa
     1801 ctggtttccg ctcacaaagt caatgcgtct tttggtcagc agctttctgt taacgattca
     1861 atagaagtca ttagtttgaa agatatttgt agagaagtcg caaaagaatt aaaagattgt
     1921 gaacgctcga atggtacgta tgattttgat cgtttttatc aaggaaaagg ggatattaat
     1981 tctcagattc ttttctctta taaagcagaa caatactaca aaggctataa aaatcatgaa
     2041 catgaatttg tacctgaagt gaaaaaggat tggaacgatc aattgaagca tgagatccat
     2101 caacaggaaa ttagaaagca aaaacgtgcg atgttgtttc aacaaggcag acaacaagaa
     2161 agggagtgag atcgtcacga tgaaaactgt cattttagca gaaaaaccgt cacaagcgaa
     2221 ggcctatgcc gatagctttt ctaaagccac ccgaaaagac gggtattttg aaatccaaga
     2281 tcgattgttt tcgggtgaaa ctgtgatcac gtatggtttt ggtcatttag tcgagttaga
     2341 ttcgcccgat atgtacgatg aaaattggaa acaatggtca ttagagcatt tgcctatttt
     2401 ccctacacat tatcactatc atgttcctaa agataagaaa aagcaattca acgtggtgaa
     2461 acagcaactt caatcggccg acaccattat tattgcgaca gatagcgatc gtgaagggga
     2521 actgatcgct tggacgatca ttcaacaagc aggcgctgat caaggaaaaa cctttaaaag
     2581 actttggatc aactccttag aaaaagaagc catttatcag ggattccagc aattacgaga
     2641 cgcagaagag acctatccca aatttgaaga agcacaagca cgccagatcg ctgattggtt
     2701 gatcggcatg aacggcagcc cactgtatag tttactcttg cagcaaaagg gcataccagg
     2761 aagtttttcc ttaggcagag tccaaacccc aacgctttac atgatttatc agctgcagga
     2821 aaagattaga aacttccaaa aagagcctta ttttgaaggg aaagcccaag taaccgctca
     2881 aaatggcata tttgatgcga agcttgatcc caatgaaacc caagcgacgc aagaagcatt
     2941 tgaagcctat ttaaaagaaa aaggtgttca agtaggaaag cagccaggca cgattcttga
     3001 agtcgaaacc gagaaaaaaa gcgcggcaag tccacgtctt ttttctctat ccagccttca
     3061 gtctaaaatg aatcagctca tgaaagctag tgccaaagat actttggaag ccatgcaagg
     3121 gttgtatgaa gggaaatatc taagctatcc acgaacagac accccttata ttacagaagg
     3181 agagcatgcg tatctgttgg atcatttaga tgaatacaag cattttttaa aggctgaatc
     3241 attccctact cctattcata caccgaatag tcggtatgtg aataataaga aagtccagga
     3301 acattacgcg atcattccta caaaaacagt tatgacagaa gcaacgtatg aaaagctgac
     3361 acctttacaa caagcgattt atgatcaagt tttaaagacg acggtggcca tgtttgcgga
     3421 aaaatatacc tatgaagaaa ctacgatttt gacacaagtc cagcagcttc agctaaaggc
     3481 agtcggaaaa gtaccagtag atctaggttg gaaaaagcta tttggcaaag agagcgacag
     3541 aaaagagaag gaagaagaac cgctacttcc taaagtaaca aaaggagaaa cggtgacggt
     3601 agatctacaa atctttgaaa aagaaaccaa gccaccacaa ccttacacgg aaggtacact
     3661 aatcaccgcc atgaagacag ctggtaaaac cgtggatagt gaagaagcac aatccatatt
     3721 gaaagaagtg gagggaattg gtacagaagc gacacgagca aatattattg aaaccttgaa
     3781 gcaaaaggaa tatatcaagg tagagaaaaa taaacttgtg gtgacgaata aaggaatttt
     3841 gctttgtcag gctgttgaaa aagaaccgct gttaacaagt gccgaaatga ccgccaaatg
     3901 ggaaagctat cttacaaaga ttggtgagca aaaagggaca caagctactt ttctagcaaa
     3961 tatccaaaaa tttgtttccc acttgttgga agtcgtccca ggacaaatcc aagccactga
     4021 ttttggagca acgttacaag aggtgaaagt agccagtgaa aagcaagaag cggcgcgtca
     4081 tttaggatct tgtccaaagt gccaggaaaa agaagtcata ctttatcaga aggtggcgac
     4141 ttgcacgtct gaagcctgtg attttaaatt gtgggtaacg attgccaaaa agaaactcac
     4201 agcgacacaa ctgaaagaga tcattcagaa cggccgaaca agtcaaccag tcagaggact
     4261 aaaaggtcag aaaggttcgt ttgaagcaac gatcgtttta aaagaagact ttacaacagg
     4321 atttgagttt tctgaaaaga agaagaccac ctacaaaaga aaagtgagac gtaaaacgag
     4381 ttagaattga cggagggaaa aacgatgaga aatacaacgc ataaagaaca agaaatcgaa
     4441 aatcaaaaag ccaaaatacg ttcagagcag gagaaaatgc gtgccagcaa acgaagacta
     4501 cagcaattga agaatcaaca acggattcaa aaagaacttg aattagatcg ataaatcctc
     4561 ctgttgttaa tattaaacta acatgttata gttatgttag tttaatatta acattcgaga
     4621 gggaaagcga tgaaagataa agtcaagtgc atttttgaga aatacaatgg taatctatcc
     4681 ttgaaggatg ctagaaatga agggttatca cctatgacgt tagatcgttt agccgaaaag
     4741 gacaaaatag aaaaaatagc atcaggtttt tataccttaa aagatagttt tgtggatgaa
     4801 ttatatctcg ctcaaagtat ctatactaga ggaatcttct ctcacgagac agcgcttgat
     4861 ctttttcaag taggaacata tatccctaaa aaaattaata tgatgtttcc taaaggatac
     4921 aatgtttcga aagagaattt agatcagtat gccattcaac cgcattacac gaataaagaa
     4981 aactttgaac tgggaatcac agaggcagaa agcttttacg gcaataaaat tttcgtctat
     5041 gatttagagc gaacgttatg tgatatgtgg aatcccagat ataaagcaag cgtagaagta
     5101 aaacaagaag cattaaaaga atatatgaca agtaccagca gaaatacaaa taagctaaga
     5161 agatacatga atattttaaa atcaccaaaa gaaatgactt tgtatatgtt gccactttat
     5221 tgagaggagt aaaggtgaat gacaccgaca caatttaaag cgaaaacaag aaatgttgca
     5281 gctgagaaac atttaaaccc acaaattgtt caaagatatt atatgatgga aaaattatta
     5341 gaatgtattg ttgaatcgcc ttatcatgat gattttgtat taaaaggggg atttctgatc
     5401 ggatcaaaat acgggttaga aaaccgttca accgttgata ttgatacgac ttatcgaaat
     5461 tcgaaactaa caaaagaaaa attagaaact gttttttctg aattaacagc tgctcctaca
     5521 aaagatggca ttcggtttga attaaagggc ttaacagaga cgagggaagc cgattattac
     5581 ccaggttttc aagcaaagtt tattgctttt cttgaaaatg cacgtgtacc ttttaaatta
     5641 gatattactt caggtgattc tattattcca gcagcattga tatatgaaca taaattgatg
     5701 tttgaagata aaacaattca tattccagct tatcctacgg aacaaatttt agctgaaaaa
     5761 ttacatgcca cgtttagttt cggaatagat aattcacgta tgaaagattt ctatgatctg
     5821 tatatgatcc ctaaactaga acgtatcgat gagataaatt tgtataagag tgttcaagga
     5881 acatttaagg aaagaaataa tcaatcagtg tttaagaatt tgtataatca ggaaatgcct
     5941 caattgatga agaatgaggg attgagggag aaatggaaaa gatatcagca agaaaactat
     6001 tttgccaaag atatttcttt cgaatctacc ttaacttctg ttgattcgct gatgcaatca
     6061 attgtcgagc aagaggaagt agaacgaaaa caaaatttta agagatcaca acaaatgaat
     6121 caagaaatgg agagataaag atgatcaata atgtaacttt agtaggtcga ttaaccaaag
     6181 atcccgattt acgttacacc gcaagtggca ctgcagtgac cacttttacc ttagccgtta
     6241 accgtaattt tactaatcaa agtggcaatc gagaagctga ctttatcaat tgtgtgattt
     6301 ggcgcaaacc agctgaaacg atagcgacat tagctaaaaa aggcagcttg atcggcgtcg
     6361 tcggacggat tcaaacgcgg acttatgaca accagcaagg acagcgtgtc tatgtgactg
     6421 aagttgtggc cgataatttc caactattag aaagtaaagc agcgacagaa agccgtatgc
     6481 acgctgatca atcgtccagc agtccaagcc ctaccacctt tgaacaacgg gatacagcga
     6541 cgcctaacaa caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata
     6601 ttagcgatga tgatctaccg ttctaagtag cattttctat tggttgcatt tgctacggtt
     6661 tatgttataa tttagacaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg
     6721 cgggctagtg gctttctttt acgtttctat tataagaatt aacg
//
LOCUS       DACCKU010000121         6764 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.125, whole genome shotgun sequence.
ACCESSION   DACCKU010000121 DACCKU010000000
VERSION     DACCKU010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.125"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..648
                     /locus_tag="IVN13_002629"
     CDS             <1..648
                     /locus_tag="IVN13_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013065.1"
                     /translation="TSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEK
                     AIPYIAAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPA
                     EVKQEHKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            709..2169
                     /locus_tag="IVN13_002630"
     CDS             709..2169
                     /locus_tag="IVN13_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP7013066.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            2180..4384
                     /gene="topB"
                     /locus_tag="IVN13_002631"
     CDS             2180..4384
                     /gene="topB"
                     /locus_tag="IVN13_002631"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7013067.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESYRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            4630..5226
                     /locus_tag="IVN13_002632"
     CDS             4630..5226
                     /locus_tag="IVN13_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7013068.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            5239..6138
                     /locus_tag="IVN13_002633"
     CDS             5239..6138
                     /locus_tag="IVN13_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP7013069.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            6141..6626
                     /gene="ssb"
                     /locus_tag="IVN13_002634"
     CDS             6141..6626
                     /gene="ssb"
                     /locus_tag="IVN13_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP7013070.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
BASE COUNT         2496 a         1166 c         1433 g         1669 t
ORIGIN      
        1 acaagtattc atgaattagc ccatgcaaga atgcacaaat tttcagaatt agataccgca
       61 actaaagaat tgcaggcaga aatgacttct tatattgtgt gcaaacattt tggaatggac
      121 acgtcggaga aagctattcc ttatattgca gcatggacga agaatggtca gacgttggat
      181 gataaggaag ctgcggaacg tgggaaaatc atgaatgatg tcagccgtgt cgcaaatgaa
      241 ttcattcaaa ccatttctaa tgaaatcaat caatacagag aaagagaacc agagatccaa
      301 ccagcagaag tgaagcagga gcataaaaat actgtagaaa cggcagaaca aaagaatatt
      361 gttcctgttg gttcactttg gatcgacaag aaagacggga aagtaatgga agaagatacg
      421 ggtcgaacgt tacatttgaa ggatgcggcg tttattgaag atccgcagca taatcaagtg
      481 attttaaaag gaaaaagtta tggaaaagag attcaaacgg cgttaccttc agaagaattt
      541 tcgctaggaa atccattccg attttcactt gaaacagtgt cagaagtggc caaacgaaaa
      601 aatgaaatct attatgaaca acaattgtca caaaaagaac gcgtataaaa caagtaaaaa
      661 agactatctg aaaaacggat agtctttttt cttttgggag gtttgaaaat gaccgaaaaa
      721 aaaatgagtc ttattgatcg atgtaaacag atcgatatag tggattttgc tcgaaacaat
      781 ggaatggctg tagtcaataa aggacgagac tatcgcttag aagaccatga ttcatttgtt
      841 tttgatcgaa gaaaacaacg tttttattgg aacagtcaaa atattagtgg cgacattatt
      901 gaattagcta aacttttttt catagacaaa gagatccaag actctaagca acaatttaaa
      961 gcagccacag attttattct aaagaatgaa gacaaaacag aacgagtaga aaatcttcat
     1021 tttgagacag aaaagtacaa agatcacccc gttgattatc aaccactcac agaaaaagga
     1081 aggaattatc tgaaggaaga acgaaaacta ccagagtggc tgattgatta tgcagaaaaa
     1141 gaagggttga ttgccgaact aaagccaaaa catgaacggc aaaatttctt agttcgagac
     1201 gatcgtttag atcatgcggt cgccttttta tggaaagatc cacagacaaa tgagacagta
     1261 ggggcgagtt atcaaggaac gttcattgat tatgaacgtt ttggtgagag aggcacatat
     1321 aagcatatag ataaaaattc gacagcgaat catggcttta acctaaagat cggtgaccct
     1381 aagcagttaa aattttttga gagtagtatt gatttactta gctatgctgc actgaatcgt
     1441 gatcagttaa acgatacgtg gttagtttcg atggaagggt taaaacacca tgtgatcagt
     1501 cattattttg gtgaggcagt atctgaattg agaaagaaac aagcctttcc tcaatccatc
     1561 gagatttgtg tggataatga ccgtgcagga catattttct acgaaaaaga acaattaatg
     1621 ggcgcagttg atccatttac gaatcagaaa gtacgttgtg aacgaggaat cgctaatgat
     1681 tggcaagtgc caaaagaata caaagtcatt tatgaagaag tcgcaaaaga agaaaagatc
     1741 acaccagaag ctattatggc catacacaag acagaaaaca atctgcaatt aacgaaccaa
     1801 ttggtttccg ctcacaaagt aaatgcgtct tttggtcagc agctttctgt taacgattca
     1861 atagaagcta ttaatttgaa agatatttgt agagaagtcg caaaagaatt aaaagcttgt
     1921 gaacgctcga atggtacgta cgattttgat cgtttttatc aaggaaaagg ggatattaat
     1981 gctcagattc ttttctctta taaagcagaa caatactaca aaggctataa aaagcatgaa
     2041 catgaatttg tacctgaagt gaaaaaggat tggaacgatc aattgaagca tgagatccag
     2101 caacaagaaa ttagaaagca aaaacgtgcg atgttgtttc aacaaggcag acaacaagaa
     2161 agggagtgag atcgtcacga tgaaaactgt cattttagca gaaaaaccgt cacaagcgaa
     2221 ggcttatgcc gatagctttt ctaaagccac ccgaaaagac gggtattttg agatccaaga
     2281 tcgattgttt cctggtgaaa ctgtgattac gtatggtttt ggtcatttag tcgagttaga
     2341 ttcgcccgat atgtacgatg aaaattggaa acaatggtca ttagaacatt tacctatttt
     2401 ccctacacat tatcactatc atgttcctaa agataagaaa aagcaattca acgtggtgaa
     2461 acagcaactt caatcggccg acaccattat tattgcgaca gatagcgatc gcgaagggga
     2521 actgatcgct tggacgatca ttcaacaagc aggcgccgat catggaaaga tatttaaaag
     2581 actttggatc aactccttag aaaaagaagc catttatcag ggattccagc agttacgaga
     2641 tgcagaagag acctacccaa aatttgaaga agcacaagca cgccagatcg ccgattggtt
     2701 gatcggcatg aacggtagcc cgctgtatag tttactcttg cagcaaaaag gcataccagg
     2761 aagtttttcc ttaggcagag tccaaacccc aacgctttac atgatttatc aattacagga
     2821 aaagattaga aacttccaaa aagaaccata ttttgaagga aaagcccaag taatcgctca
     2881 aaatggcgca tttgatgcga agcttgatcc gaatgaaacc caagcgacgc aagaagcatt
     2941 tgaagattat ttaaaagaaa agggggttca attgggaaaa cagccaggta cgatccacca
     3001 agttgaaacc gagaaaaaaa gtgcggcaag tccacgtctt ttttctctat ccagccttca
     3061 atctaaaatg aatcagctca tgaaagctag tgccaaagat actttggaag ccatgcaagg
     3121 gttgtatgaa gggaaatatc taagctaccc acgaacagat accccttata ttacagaagg
     3181 ggagtatgcg tatctgttgg atcacttaga cgaatacaaa cactttttaa aggccgaagc
     3241 cattcctact cctattcata caccgaatag tcgatatgtg aataataaga aagtccagga
     3301 acattacgcg atcattccta caaagacggt catgacggca gcggcttttg aacaattaag
     3361 tccactgcag caggctattt atgaacaagt gttaaaaaca acggtggcca tgtttgcgga
     3421 aaagtatacc tatgaagaaa caacgatctt gacgcaagtt cagcagcttc agctaaagac
     3481 agtcggaaaa gtaccagtag atctaggttg gaaaaagctg tttggcaaag agagctacag
     3541 aaaagaaaag gaagaagaac cgctacttcc taaagtgaca aaaggagaag cggtgacggt
     3601 agatctacaa gtccttgaaa aagaaacgaa gccaccacaa ccttataccg aaggtacgtt
     3661 gatcacagcg atgaaaacag ctggaaaaac cgtggatagc gaagaagcac aatccatatt
     3721 gaaagaagta gaaggaatcg gtacagaagc aacacgagca aatattattg aaactttgaa
     3781 acaaaaggaa tatatcaagg tagaaaaaaa taagcttgtg gtgacaaata aagggatttt
     3841 actttgccaa gccgtggaaa aagagccgct gctaacaagt gccgaaatga ccgccaaatg
     3901 ggaaagttac ttattaaaga ttggtgagca gaaaggaaca caagctactt tcttagctaa
     3961 tatccaaaaa tttgtctctc acttgttgga agtcgtacca ggacaaatcc aatccactga
     4021 ttttggatcg acgttacaag aggtgaaagc agccagtgaa aagcaagaag ccgcgcgtca
     4081 tttagggatt tgtccaaaat gccaggaaca agaagtttta ctctatcata aggcggccgc
     4141 atgtacgtct gaagcttgtg attttagatt atggacaacg attgccaaaa agaaactcac
     4201 agcgacacag ctgaaggaaa tcattcaaaa tggccgaaca agccagccag tgaaaggatt
     4261 aaaaggtcaa aagggttcgt ttgaagcaac gattgttcta aaagaagact tcacaacgag
     4321 ctttgaattt tctgaaaaga aaaaaacgaa ctataagaaa agaacacgcc gcactacaaa
     4381 ataatggagg ggttatctta tgcgaaatac aacacacaaa caacaagaaa tcgaaaatca
     4441 aaaagcaaaa attcgttcag aacaagagaa attacgtgcc agtaaaagaa gacttcagca
     4501 attaaagaac cagcaacgga ttcaaaaaga aattgaatta gatcgataaa atcttcctgg
     4561 ctattgtata cgcataaact tatgttatac tatatttata agtttatacg tatacaaagg
     4621 agggagaaaa tgacaaaagc aatggtttac aagctgctag aagaatttaa cggaactttg
     4681 gctttgaagg atgccaaaaa agcgggcatt tctcctgtaa cgatcaaacg tatggtagat
     4741 cgtggagagc tggatcgtga gtatccaggt tttttcacct tacctggtca atttcctgat
     4801 gaattgttta tggcacaaaa gaaatatgag cgcggcatta tttcgcatat caccgcgttg
     4861 gatttatatg atttaacgga tatgatccct agacagatcg atctaaccgt accttacggt
     4921 tatcacgttt ctgaaaaggg gttgaaagaa ttcgcagttg agttacacta ttcaaaagcg
     4981 gaatggtatg aattaggtaa gatcgaaata aagagtcgtt atgggaatcc tgttatagct
     5041 tatgatccag aaagaacctt gtgtgacatt tggaatccct ggtacaacgt tgaggatgaa
     5101 atcaaagtaa aagcaatcaa aaactacatg gagtcggaca gaaaaaatct tagaaaattg
     5161 aatgagtatc gacgaatttt gcctacagac aaaacgatac gctcttatat tatggcttta
     5221 aattaaagga gaagacacat gacacctacg caattaaaag caaaagtgaa gaatatttcc
     5281 agagaaaaag aagttgatcc gcagcttgtt ttaagacatt ttatgatgga aaaattcttg
     5341 gaaaaaatct cagagtctcc ttatcgtgaa aattttgtgt taaaaggcgg cttcttaatt
     5401 ggctcaaagt atggcattga aaatcgaaca acaaaagaca ttgatacgac tttacgggaa
     5461 atgaaagtaa ccaaagaaac attgacaacg gttctaaacg atattttttc aacgccgacg
     5521 aaagaaggca ttcaatttga gattcaagga atgaaagaaa caagggaagc tgattattat
     5581 ccagggttta gcttacgtgt actggcacat ttggaaaaca tgcggccaga ttttaaggta
     5641 gacgtgacga caggtgactc gatctatcct gcaacgatca ctcacagtca taaattgatg
     5701 tttgaaaatc gaacgattcc tttggaatct tatcctacag aacaaattat tgccgaaaaa
     5761 ttaagtgcaa cctttgattt tttaacagat aattcgagag gaaaagattt ttatgatctc
     5821 tatactattc ctaaaatgga aaagatcgat gagaagacct tgaaagatag tgtgagaaat
     5881 acatttacac gtcgaggaaa aactgatccg ttaaagattt attacgaaaa ggatatggat
     5941 gtactgaaaa atagtcatga gctaaaagat acctggaaga aatatcaaaa aagcaatcct
     6001 tatgctgcgt cgatcacgta cgaagagaca atggattcga ttagtaattt gatggaaaaa
     6061 gtgattcacg tcgaagaaaa agagcgaaaa atagcttatc aaagacacca acagatgaac
     6121 agagaaatgg agagataaaa atgatcaata atgtaacttt agtaggacgg ttaaccaaag
     6181 atccagattt acgttatacc gcaagtggca cagcagtggc tacttttacc ctagccgtta
     6241 atcgtaattt cacgaatcaa aacggcaatc gagaagctga ttttatcaat tgcgtgattt
     6301 ggcgcaaacc agcagaaacg atggcgacat tagctaaaaa aggcatcttg atcggcgttg
     6361 tcggacggat tcaaacacgg acttatgaca accagcaagg acaacgtgtg tatgtgacag
     6421 aagttgtagc tgataacttc caactattag aaagcaaagc agcgacagag agccgtgcgc
     6481 acgctgatca atcgtccacg agtccaagca ccaccacttt tgaacaacgg gatacagcga
     6541 cgcctaacaa caatggattg aacgcttcac agaatccatt tggtggacaa tcaatcgata
     6601 ttagcgatga tgatctaccg ttttaagtag cattttctat tggttgcatt tgctacggtt
     6661 tatgttataa tttagtcaac aaaaagaatt ttcaatctat aaccaaaaac cactagctgg
     6721 cgggctagtg gttttctttt acgtttctat tataagaatt aacg
//
LOCUS       DACCKU010000122         6583 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.126, whole genome shotgun sequence.
ACCESSION   DACCKU010000122 DACCKU010000000
VERSION     DACCKU010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.126"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..826
                     /locus_tag="IVN13_002635"
     CDS             <1..826
                     /locus_tag="IVN13_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7013071.1"
                     /translation="KEIGRLSYAAKRSSNWSNKVEKSKYGTTNSGSKLDKGYVGHKAA
                     KAMKRAKNIESRHQEAVLQKSELLHNIEQYDDLKISPLEFHKECLIEANDLSLSYGDK
                     EVCSNLNFRVNIGDRVAIIGKNGSGKSSILKLINGDDIKFTGNFMLASGLKISYISQD
                     TSYLKGNLSEFAYNNKIDETLFKTILRKLDFNREQFDKNMVDFSAGQKKKVLIAKSLC
                     ESAHLYIWDEPLNYIDIFSRIQIEKMILEYCPTLLFVEHDDAFCNNICTKNINLGL"
     gene            880..1683
                     /gene="lnu(B)"
                     /locus_tag="IVN13_002636"
     CDS             880..1683
                     /gene="lnu(B)"
                     /locus_tag="IVN13_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAP7013072.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            2371..3192
                     /locus_tag="IVN13_002637"
                     /pseudo
     CDS             2371..3192
                     /locus_tag="IVN13_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            3335..3559
                     /locus_tag="IVN13_002638"
     CDS             3335..3559
                     /locus_tag="IVN13_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7013073.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGDEIKISNE"
     gene            3574..4443
                     /locus_tag="IVN13_002639"
     CDS             3574..4443
                     /locus_tag="IVN13_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7013074.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            4424..5158
                     /locus_tag="IVN13_002640"
     CDS             4424..5158
                     /locus_tag="IVN13_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7013075.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            5191..6099
                     /locus_tag="IVN13_002641"
     CDS             5191..6099
                     /locus_tag="IVN13_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7013076.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            6096..6344
                     /locus_tag="IVN13_002642"
     CDS             6096..6344
                     /locus_tag="IVN13_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP7013077.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
     gene            complement(6347..>6583)
                     /locus_tag="IVN13_002643"
     CDS             complement(6347..>6583)
                     /locus_tag="IVN13_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP7013078.1"
                     /translation="PRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVK
                     GLESRFESILLAYSVLLSRAQRRFGGTLRYSLGY"
BASE COUNT         2282 a          960 c         1377 g         1964 t
ORIGIN      
        1 taaagaaata ggaaggttgt cttatgcagc aaaacgtagt tcaaactggt caaataaagt
       61 agaaaaaagt aaatatggaa caacaaattc tggttcaaaa ctggataagg gttatgttgg
      121 acataaggct gcaaaagcga tgaaacgtgc caaaaatatt gagtcaagac atcaggaagc
      181 cgttttacaa aaatcagaac tgctccacaa cattgaacaa tatgatgact taaaaatttc
      241 accacttgaa tttcacaaag agtgcttaat agaagcgaat gatttatcat tgtcttatgg
      301 agataaagaa gtatgcagta atcttaattt cagagtcaat attggtgata gagttgccat
      361 tatcggaaaa aatgggagtg gtaagtctag tatcctaaaa ttgattaatg gagatgatat
      421 taaatttacc ggaaatttta tgctagcaag tggactaaaa atttcttata tttcgcaaga
      481 tacttcatat ttaaaaggta atctatctga atttgcctat aataataaga tcgatgaaac
      541 tctatttaaa acgattcttc gtaaactgga ttttaataga gagcagtttg ataagaacat
      601 ggtggatttt agtgctggtc agaaaaagaa agtactaatt gctaaaagcc tttgtgaaag
      661 tgcacatttg tatatatggg atgagccatt gaactatatt gatatttttt cacgtatcca
      721 aattgaaaaa atgattttgg aatattgtcc tacactattg tttgtggagc atgatgatgc
      781 tttttgcaat aacatttgta cgaaaaatat taatttaggt ttgtagagat tttgagttgc
      841 cacaataact aaaagatatt tacatgaaag ggtgaagaaa tgttaaaaca aaaagaatta
      901 attgcaaacg ttaagaatct tactgagtca gatgaacgaa ttacagcttg tatgatgtat
      961 ggatcgttta ccaaaggaga aggtgaccaa tactctgata tagagttcta tatatttttg
     1021 aaagatagta taacctcgaa ctttgattca tccaactggt tgtttgacgt agctccgtac
     1081 ttgatgcttt ataaaaatga gtacggaaca gaggtagtta tttttgataa tcttatacgt
     1141 ggggaatttc atttcctttc tgaaaaagat atgaacataa tcccctcgtt taaagattca
     1201 ggttatattc ctgatacgaa ggctatgctt atttacgatg aaacagggca attagaaaat
     1261 tatttatcag agataagtgg tgcaagacca aatagactta ctgaagaaaa tgctaatttt
     1321 ttgttgtgta atttctctaa tctatggttg atgggaatca acgttctaaa aagaggagaa
     1381 tatgctcgtt cattagaact cttatcacaa cttcaaaaaa atacactaca acttatacgt
     1441 atggcagaaa aaaatgctga taattggcta aacatgagta aaaaccttga aaaagaaatt
     1501 agccttgaaa attataaaaa atttgcaaag accactgctc gattagataa ggtagaatta
     1561 tttgaagcct ataaaaattc tttgctatta gttatggatt tgcaaagtca ccttattgaa
     1621 caatacaact taaaagttac acatgacatt ttagaaagat tgttgaatta cattagtgaa
     1681 taggagagta acgttattca cagtattggg tactgaaaat taacagcata cctcagtttt
     1741 tcaaaaaaca gcgaagccta tggatatgtt cccgtctacc gatagtgcaa aaagtgtcgt
     1801 ggatatgttt ccgagaacgg acatacacaa atgacattca ttctgtcctc tcaatccatg
     1861 tggaagcagt atgtctgtta acaagaaaat aattattgtt cgtcctgacg agttgttaga
     1921 ctcgaaaagg ataaacatca tttttagaac cggtagccga tttttggcta gcggtttttt
     1981 gggctctttg gcaaatagtg gtgattgaat ctcgcgctat cctatggaaa accgccttgt
     2041 gtgaggggct gcgagaccgc ttcgatccta gaattgctga agcacgaatc tagcgtcact
     2101 cacatttcat cttttgacgt agctgagaca aggtgttttt cttcgatccg ctagtggtta
     2161 atctcttttt ggtgcaaata tggtttagaa tttctttgca tggcttaagg gagtggagaa
     2221 tcggatatta gtaggattta catccacaga taaaaatagg cttgatttta acagcgaaac
     2281 taaaataccc tacttaaatg aagtgataga ttagatgttg ggttgttagg agatgcttaa
     2341 aaatcctatg caatcaatag tttttagtgt ttgttgactt gatccctaaa tcgcctgagt
     2401 actaaacgtt gagtgtgtct gcttactgtc actagccagt aagtgagact gtagttaatc
     2461 tctcattttc cccagtatta tgttgaaaat agccatgaag cgattatttc taaagagtta
     2521 tttttgcagg ctcaagaaga aatttatcgg agaagtaata tctacacagg agaaggcaag
     2581 aacaaaagaa tttatagtag caagtacgct ttaagtgcca tcaccttctg tggatattgt
     2641 ggcgatattt ataggagaac ctattggaat attcatggca gaaaagaatt tgtctggcga
     2701 tgcgtgacta gaatcgagca gggtcccgag gtatgtaaga accgaaccgt aaaagaagat
     2761 gaactctatg gtgcggtaat gaccgcaatt aataagctac ttgctggtgg caacaatatg
     2821 ataaagacac tggaagaaaa tattcatgct gtgattggag aaaccacaga ataccaaatt
     2881 tcagagatta ataccctact agatgaaaaa caaaaagaac taatcaagct ggccaataaa
     2941 ggtcaagatt atgaatacct ggtagatgag atcgatgaga tgcgagataa gcgtcagact
     3001 cttttagtag aagatgcctc cctcagtggc gagaacgagc gcatcaatga gttgattgaa
     3061 tttatccgca agaacaaatt ccgcacctta gaatatgatg ataagcttgt aaggaagata
     3121 atccagaacg ttaaggtcta tgaagaccac ttcgtcatag ccttcaaacc aggtatcgaa
     3181 atggaaatat gaaaaccaga agtaaatagc ccatgactct gaagtagagt tgtgggtttt
     3241 cttttaattg ctgattataa taatatttta atgactattg catttatcaa ccaaatggtt
     3301 tatgttattc ttagcataaa cttggaggtg ctgtatgact acggcagaaa tgattaaaga
     3361 actgtgtgag caaatgaata taagtgtttc cgaacttgct agacgtattg gccagactcc
     3421 acagaatttt aataaaaaat tacaacgaga aacagtaacc ttggatgagt tgaaggccat
     3481 tgctgatgtg ctaggtgtca agtttgtgca ggcatttatt ttaccagatg gagatgaaat
     3541 aaaaatatct aacgaataaa ggaggatgtt tatatggtag gtaatgttat tcaaatagta
     3601 acagagaaat tatcatcttt gccttttata gaaggcattg tattaggggg ctcacgtgca
     3661 agaagcaccc atacagaaaa ttctgatata gatatcggaa tctattacaa ttcagactca
     3721 tttgacctga cagcgattaa tcaaattgct acagagttgg atgatgagaa tagaaacaac
     3781 cttgttgtac ctcccggagc gtggggtgat tgggttaatg gcggtggatg gttagttata
     3841 aacgggtgtc atgttgactt gatattacgt gatataaaac gggtggaaca aataatcaaa
     3901 gatacggagc aaggaattgt tactgccaat tatcagaccg ggcatcccca tggttatata
     3961 agtgctatgt atcgtggaga attagcgatt agtaaaatac tatatgctaa gaacgaaagc
     4021 ttatgcgaat taaaaaagca ggcagaaatt taccctactg ctctaaagaa aagcttgatg
     4081 aactttttta tatttgaagc tgaattctct ttaatgtttg taaaagcaaa tgcgggggca
     4141 gaggataaat attatattgc aggccatgtt tttcgtatta tttcatgctt aaatcaagta
     4201 ctatttgcat gtaataatgc ttattgcatt aacgaaaaga aagctataaa actgcttgaa
     4261 acttttgaat ataaacctga aaaatatgcc gaaagagtta atcatatttt tgaagtactc
     4321 ggtctttctc tttttgaatg ctacgacatg accgagaaac tttataaaga agtgaatgaa
     4381 attgtatcgg agataaataa ctttttaaac gaggagagtt cagatgaaag aaaacaaata
     4441 tgatgataat atattttttc aaaaatacag tcaaatgagt cgctcgcaga aaggactggc
     4501 tggtgcggga gaatgggaga ctttgaaaaa gatgctacct gattttaagg gtaagcgtgt
     4561 gcttgattta ggatgcggct atggatggca ctgtatatat gcgatggaaa acggtgcttc
     4621 ctctgtagta ggtgttgata tttctcataa aatgctcgaa gtagcaaaag gaaaaaccca
     4681 ttttccacag attgaatatg aatgctgtgc catagaagat gtggatttcc cagaggagag
     4741 ctttgatgta atactaagtt cgcttgcgtt tcattatgta gcagactatg agaatttaat
     4801 aaaaaagata tataggatgc tgaaggctgg tggcaattta gtttttacag ttgaacatcc
     4861 tgtttttact gctcatggaa cacaagactg gtattataac gaaaaaggag aaatactgca
     4921 tttcccggtg gacaattatt attatgaggg caaacggaca gctatgtttt tggaagaaaa
     4981 ggttacaaaa tatcatagaa cactgaccac atatctaaat acactgcttt caaatagttt
     5041 tataataaat cagattgtgg agccacagcc gccagagaac atgatggata ttccggggat
     5101 ggcggatgaa atgcgacgcc caatgatgct gattgtatcg gcaaaaaaga agatgtaata
     5161 atatagaaaa aataaacgag gagtatgtaa atgagatcag aaaaagaaat gatggattta
     5221 gtactttctt tagcagaaca ggatgaacgt attcgaattg tgacccttga ggggtcacgc
     5281 gcaaatatta atatacctaa agatgaattt caggattatg atattacata ttttgtaagt
     5341 gatatagaac cgtttatatc taatgatgac tggcttaatc aatttgggaa tataataatg
     5401 atgcaaaagc cggaggatat ggaattattc ccacctgaag aaaagggatt ttcctatctt
     5461 atgctatttg atgattacaa taaaattgat cttaccttat tgcccttgga agagttagat
     5521 aattacctaa agggcgataa attaataaag gttctaattg ataaagattg tagaattaaa
     5581 agggacatag ttccgactga tatagattat catgtaagaa agccaagcgc aagggagtat
     5641 gatgattgct gcaatgaatt ttggaatgta acaccttatg ttattaaagg attgtgccgt
     5701 aaggaaattt tatttgctat tgatcatttt aatcagattg ttcgccatga gctgctgaga
     5761 atgatatcat ggaaggtcgg catcgaaaca ggctttaaat taagtgtagg caagaactat
     5821 aagtttattg aaaggtatat atccgaggat ttgtgggaga aacttttgtc cacctaccgg
     5881 atggattcct atgaaaacat atgggaagca ttatttctat gccatcaatt gttcagggcg
     5941 gtatccggtg aggtggcgga aaggcttcat tatgcctatc cggagtatga taggaatata
     6001 acaaaatata ccagggacat gtataaaaaa tacactggta aaaccggctg cctggatagc
     6061 acatatgccg ctgatataga agagaggcgg gaacagtgat tacagaaatg aaagcagggc
     6121 acctgaaaga tatcgataaa cccagcgaac catttgaggt gataggtaag attataccga
     6181 ggtatgaaaa cgagaattgg acctttacag aattactcta tgaagcgcca tatttaaaaa
     6241 gctaccaaga cgaagaggat gaagaggatg aggaggcaga ttgccttgaa tatattgaca
     6301 atactgataa gataatatat cttttaaccc gtggtgctag ttaattttaa tatcctaaag
     6361 aatatctcaa agttcctcca aaacgtcgtt gtgctcgact taatagaaca ctgtaagcca
     6421 gtaatatgct ttcaaatcta ctctctagcc ctttaacaga acgtgagttg aaattttgac
     6481 aacctaattt ttctaaggaa gaaaaaacag tctcaactgt ttttctttgt tttccaagta
     6541 agctgtggtc tagtttttct gatttatcca tgtttttacg tgg
//
LOCUS       DACCKU010000123         6547 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.127, whole genome shotgun sequence.
ACCESSION   DACCKU010000123 DACCKU010000000
VERSION     DACCKU010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.127"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(23..604)
                     /locus_tag="IVN13_002644"
     CDS             complement(23..604)
                     /locus_tag="IVN13_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP7013079.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(610..1869)
                     /locus_tag="IVN13_002645"
     CDS             complement(610..1869)
                     /locus_tag="IVN13_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP7013080.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(1871..2917)
                     /locus_tag="IVN13_002646"
     CDS             complement(1871..2917)
                     /locus_tag="IVN13_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013081.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(3152..3427)
                     /locus_tag="IVN13_002647"
     CDS             complement(3152..3427)
                     /locus_tag="IVN13_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP7013082.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(3670..4980)
                     /locus_tag="IVN13_002648"
     CDS             complement(3670..4980)
                     /locus_tag="IVN13_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP7013083.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(5162..6391)
                     /gene="rpsA"
                     /locus_tag="IVN13_002649"
     CDS             complement(5162..6391)
                     /gene="rpsA"
                     /locus_tag="IVN13_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP7013084.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
BASE COUNT         1844 a         1403 c         1056 g         2244 t
ORIGIN      
        1 tttagtacta tactattttt aactaattgt tatgttcact tacccggtta cttttctttt
       61 gtgtctgcaa aaggatcttt cggttaactt catcagataa tttcaaaaaa gcatccctat
      121 ctccatcttc tagtgcttga tcgatttgag cataaagcat ttttagtttt gcctcttctt
      181 ctttttcttg aaaatatcgt tcaattaatt cagtatcttc ttcactgact gaagaatccc
      241 aagaagaaaa tggattgtct tctaagatag atagatattc tcttgtctgc caagcatttt
      301 cgaatataca ttccacatat aaaggctttt tccaattcaa acggatttca tgaaaaatct
      361 ggtctgtgtc cgtaaattct tttccctgca aaaacaattt catgggttcc ccttcaacag
      421 atagatcact gatctgcagt cctcgatccg caaaattcgc cccttcaaca aagtggacat
      481 tgcttaaaat cgcttcatga ttggcaagat aattcaagat ccaaaaaact tctcttcggc
      541 taaaagatac atgattgacc atccacgtta aaaaattctt cttttcccgt acatcaatta
      601 gcatttttct cacctctctg ttaggtcatc taataatgac agtgcctcca tatctcctgg
      661 ttcaagtgtt aaataataag tcaataattc ttgcgctctt ttcagctgcc cttcttctct
      721 taagaaaaag gcatactctt tcaagaaatc tggttcatgt gatagttcat gataagcctg
      781 ttcatagtgt acagctgcaa cggtaaagtc ttctaattca ttataagcat gggctaggtt
      841 ccattctgca taaggattag cagtttcttc catctgattg atcacttgta tcacatcttc
      901 atatctttca tcatccaagt aaagattact taaagtaagt aatgtttcat cttgtttgtc
      961 tcctacttct aaagctttca gtaaaagttc ttctgctcgc tgtctatcat gcagacggaa
     1021 agcattttcc gaggccaggt gataaagttc gacttggtaa ggattttctt tgattccttc
     1081 ttctaacact gtttgtgctt cttcaatcat ttcttcttct tgaagagctt gtcctagata
     1141 tagatataac gattgataat gcggattaac ttctcgaagt tcttccagat agttgattgc
     1201 ctgctgattt tctttcaatt gaagattcgt aaaagctagc tggaacagtc gatcatctgt
     1261 cctttcttct ttcagtgctt cttctaaagg tacgatagct gcttcgaatt ctccttgcat
     1321 acttaaggat tgtcctaaac gttcctgcat cgagattcct gaaatttcat ttatcccgct
     1381 tgccaacaat gtttcataaa ccactgctgc ctctttgaaa cgatcaactg aaaaatacaa
     1441 ttcagctagc gcaaactgga tcaatggttc atcaggaagc aacccagctg cttctttcaa
     1501 ttttgcttca ctcacttcag ggattccgat aacttggtac aaatcagcta tcgccaataa
     1561 gctttgcgga tagtaatcac tttgcttatc cactttttcc aggtattcaa aagctaagtc
     1621 gatctcatta tcttcgattg caatttctgc taacgggatg ttcagtccgt cattttctgg
     1681 gtattggttg atcagttgtt caaaaatctg ttttgcttcg atcaaaaagc caatagccag
     1741 caattcttcg cctagttcag ctaatatatc tgctgggtct tcttttaatg ctttttctaa
     1801 aaagatttgt gcttgtgaca agtcttcatc tgataaagct tgcaacattt tttcgctatt
     1861 tgtattcatt tcaattccct cttatctcac tgacgatttg ttgaaatacc gtcattgttc
     1921 tcaataattc tttttcgttt ggatgttggt atccgtctat tatacctatt ttttctaaaa
     1981 caagtatttt tttgcttttt tctgccaaaa aaagaacttt ttccaaatac tccatctttg
     2041 aaatctgttc cagccagtgg attggatagc caagcaagtc cagccacaca aaaaagtttt
     2101 ttagttggaa atcaaaagga tatgcttgga gattccacac gaaatgaaac atcattccta
     2161 aaaatttttt catactagca gaaagtagat ggccattttc ggtcagataa aaagcttgtt
     2221 caaacagttt accataattt gacaggtttt ctgcctctaa ttgataaaaa tagatcattt
     2281 cttcgatcat cccggcaaac ggccgagtcg ttaattgctt tttatttgga tagtttttat
     2341 acagattttg caaaaaacga tgatcacaaa tcattccgca acagataaaa gtcaaaaagt
     2401 caatctgctt tccgtccacc tgccgctcgc tgattgtttg atcataaacg atgcgttctg
     2461 gcagattgat ggtttgcagc actacacgat ttggtagctg caaaatcgtc cgctgcggtg
     2521 ttaatgctcg gcttagagaa cggatcgata cagggatcat ccatagtgat gaatcaaggg
     2581 ttgtatgttt ttgaaaaaaa cctgccagat ccatgatccc ttcatttcca aatccgatga
     2641 taaacatgga ttgattttcg ggatagcgtt tgacgaaatc aagcaggttc tttaattctg
     2701 ttaaatgatt gcattgagtg ttggcacaaa tataccagtc attgtttgct tgatttgcaa
     2761 ataacgggtt gatcttatct gcaaacaaat cataatagcg ctgattcgtt aaaaataaga
     2821 ttggctgatc aatggaaagt tctcttagct gactgactag cgtttctccg taaaacactt
     2881 tcgtcaacgc atcttctact tgataatcaa gttccatacg catccctcct cttcttattt
     2941 ttattttacc attagtaagc aaattctaga aaagataaga gcaaagctca cctcataact
     3001 tgcaaataaa taaaaattaa ataaaataca tgtgttttct atgtatgaca tacggattcg
     3061 gaatctctgt ctcttattta ttccttttct gctaggaaat aaaaagaaaa gcacttgtag
     3121 ccctagagcc acaagcgctt tttcaactgt cttatttaac agcgtctttt aatgctttac
     3181 ctggtttgaa tgcaggtact ttgctagctg gaatttcgat ttcttcacca gtttgtgggt
     3241 tacgaccttt gcgagctgcg cgttcgcgaa cttcaaagtt accaaaaccg attaattgaa
     3301 ctttttcgcc gttagctaaa gcatcttgaa tagttgaaaa tacagcatct actgctgcag
     3361 ttgcatcttt tttagttaaa tcagtagctg aagcaacttt ttcgattaat tctgctttgt
     3421 ttgccatgga tagattcacc tcctccacaa gagatatgat agaaatatca tttagttaaa
     3481 actttctgag tggtccagaa gtttaaaaag aaatattgat taacttcaat attctgttat
     3541 cacgatatca tatgaaccgc ttaatagcaa ggtttttacc aattttcaca tgcttttttt
     3601 agtcatagaa acgtaacaaa cgaaactgtc tttttctttt gaaaaaaaaa cgtcgatatg
     3661 gtaaaatgcc tattttctcc tacgagggat aattcggatg gacgtccctt caaaagtaaa
     3721 tgcttttctg atttgatttt ctaagaaacg ttcataagaa aaatgcatca attcctcttc
     3781 attgacaaaa acgacaaatg taggtggttt gacagcgact tgtgtagcat aaaaaatctt
     3841 caggcgtttt cctttatctg ttggtgttgg attgattgct acagcatcca taatgatatc
     3901 attcaacaca gccgaagaga tacgaagatt ctggttcata cttacttgtt cgatcatttc
     3961 aggcagacgt tccaaccgtt gtttagtcac tgcagaaaca aaaacgattg gagcgtaatc
     4021 aagataccgg aattcatcac gaatctcttg ttcgaaatca cgcattgtat tggtttcttt
     4081 ctttacaaga tcccatttat tcacgacgat aataattcca cgaccagctt catgggcgta
     4141 acccgctact cgcttgtctt gttcccgaat cccttcttca gcattcagta ccatcaaaac
     4201 gatatcagaa cggtcaatgg cacgcattgc acgcatcacg ctgtattttt cagtattttc
     4261 gtatactttt ccgcgtttac gcataccagc tgtatctatc attaagaatt tctgaccact
     4321 ttcagaaacg aagtgggtgt cgattgcgtc tcgggtcgtt ccttcaatat ccgaaacaat
     4381 tacacgttct tctcctaaaa tcgcattgat caaagaggat ttaccaacat ttgggcgacc
     4441 aatcaaacta aacttaatcg tgtcgtcgtc ctcttcttca gtctcttcag agaaatactt
     4501 cactgcttca tctaaaatat caccaatccc tagtccatgg cttcctgaaa caggataagg
     4561 atcgcctagc cctaaggaat aaaattcata gatatcgttt ctcatttctg gattatctac
     4621 tttattgact gctaaaatca ctggtttatt acttctgtat aaaattttcg caaccatctc
     4681 atctgcgtct gtcacgcctt cgcgtccgct gactacacaa atgattacat ctgcttcttc
     4741 aatcgcgatt tctgcttgat gcttgatctg atccataaaa ggttcatcgc caagatcaat
     4801 tccgcctgtg tcgatcacgc tgaattctcg acctaaccat tcaccttttg cataaatacg
     4861 atcacgagtt actccaggag tatcttcgac aatcgagata cgctcaccag cgatgcggtt
     4921 aaaaatcgta gacttaccga cgttcgggcg gccaacgatt gcaattgttg gatttgccat
     4981 attatttcct cctcacaagt ttccaatctc acttagttta acgaatgcat tgccaacttt
     5041 caagcataga ttgtcaatta aacgaagtat ttttattcct tattgaaata ctaatcgctt
     5101 ttcaaaagaa aaactgagac aaaagcagct gctttcatct cagtcacaga tacaaaatca
     5161 attatttttc agaatcatcg gttgcttttt cttctacttc ttctttcaac gcatcaccta
     5221 aaatgtctcc catagtaaat ccagtatttt cttctggtaa ttcataagac tcaacatttt
     5281 ttggttcttc ttctgaagct ggtttttctt ctaaagcttt gatactcaat gcaatacgat
     5341 gttcttctgg atggatctct aatactttca ctttcacttc gtcaccttca tgaagcactt
     5401 catgtggtgt cgcaatatgt ttatgtgaaa tttgagagat atggactagc ccttctactc
     5461 cagggaacac ttcaacaaag gcaccgaagc ttgtcaaacg tttaactgtt ccgtctaata
     5521 ctgtgccaac tggagctttt gtatcgattt ctgaccaagg tccaggctgt gtgtctttga
     5581 ttgacaagga aacacgttct tgttcaggat tgatagacaa cactttgaca tttacatcat
     5641 cattgacagc caatacatca cttggttttg ctacatgact atgtgaaatc tctgagacat
     5701 ggactagacc gtcaattccg cctaggtcga caaatgcgcc aaaatcagta agacgtgcaa
     5761 cacgtccact gagaacatct ccctcatgga tagttgaaag aatttctttt ttcttttctt
     5821 ctctttcgtt ttccactacc gctttatgcg ataagatcaa acgattttct gatggttcga
     5881 tttcgatgat tttaaagctc aatgttttgc ctttatagtc tgaaaagtct gcaacaaaat
     5941 gatcttccac cattgatgca gggacaaaac cacgtacacc gacgtcaacg actaaaccgc
     6001 ctttgacaac atttgtaaca ggtgcttcga tgaccttgcc ctcttggaaa tctctttcga
     6061 tatcttccca aactttttta gcatctaaac ggcgttttga aagtagatag ctgccgtttt
     6121 ctttatcttt gccgatcgta ctgataacga ctaaatctaa tacatcaccg acttttacta
     6181 cttcgctgat atcttctacc ggcaaagttg ataattcttt tgccggaacg actccttcaa
     6241 ctccagcccc ttcgattccg acaatcactt gtttgtcttc aatgactaaa acttcgccct
     6301 taacaacatc gccaactttt acttcttgta cgcttttcat tgcgtcttcc attgactggt
     6361 tgttctcttg attttgatca aattctgtca taacacttgt cctcctaacc aacattctgc
     6421 cgtaagtacg gtactgtttt tagtttaatt gattattcaa aaaaaataaa gcgatttcgt
     6481 tcgttttcct tgaaaaaaca attgtttttc attttttttc ttaatttaaa aaagaagata
     6541 acctttt
//
LOCUS       DACCKU010000124         6499 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.128, whole genome shotgun sequence.
ACCESSION   DACCKU010000124 DACCKU010000000
VERSION     DACCKU010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.128"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            165..2108
                     /locus_tag="IVN13_002650"
     CDS             165..2108
                     /locus_tag="IVN13_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7013085.1"
                     /translation="MLSKRESQLIELLLEEENFQPASFFSEKLNVSNKTIYQDIQKLG
                     QFLNRFELKIEKKPRMGIYLIGTSTKKNDAKIFITSKEAMSTKVKYSSNYRRVYLFAN
                     LIFKTDKKRYEYFEKKFFVSIATLRKDYEEIIKYCRLAGIRITSNHVIDTQKTPEELI
                     QRTFVNYFEKNIGVGNEKCQYHFVFDQAMVKFVDDFVKNFYQQSKQQPTNYIVHSLKL
                     SLLTLIGRVKHGYHSQTNKNPLFVEIKKMELYMVALDFSNGVKDELNCSFTINDLYFI
                     CSLFMGHGVRPFVNLATHSQQIMETTKQFIQDMSTLVEKDLGQDSQLLQSLFAHIEPM
                     VYRLQMGITIKNPLKEDIIRQYSTMYTLATYCIPKLEKAMGIRVTDDEITFLTIHFQL
                     AFEKIVNTKHVFLICPTGLGTSQLLFQRIRHSSPSTVVYEVIDVQKLQDYNLDSVDLI
                     ISTVKLENEYQTPVIYVRPLPTKEEIALINRHLLTLNQQDNTFLPSIAPDKTILKKYL
                     KDGWMFLNQDFDSRESVLSFITEKYKEEQLVKDNFIEVVLQREKLGATGLSSGVAIPH
                     ANPKYVVKTKISIITLKNKITWGDVDIRVVLFLAIAEEDMIQVKNMIALLYDVFVSPE
                     RIKSIASKETRNEIINEIIGKRK"
     gene            2110..2571
                     /locus_tag="IVN13_002651"
     CDS             2110..2571
                     /locus_tag="IVN13_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7013086.1"
                     /translation="MTSYFGKKIALFQSEVKSQKEALSIIATCLYQTGLVKDSFAENV
                     LKREEVFPTGLLINDVGIAIPHTDAEHVNDSQIAFMSLKTPVTFYEMGTADKEISVSL
                     IFMLALKEPHAQLEMLQKLITMFQKKEVVQKLRAVNNTEEYYQIIEKEGFV"
     gene            2595..3941
                     /locus_tag="IVN13_002652"
     CDS             2595..3941
                     /locus_tag="IVN13_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP7013087.1"
                     /translation="MLDLLQKFVDLGSIVVLPILIFVFGLILGTKPSKAFISALTVGV
                     GFVGLNLVIDLLTNSLGPAAQAMVERFGLNLTTIDVGWPAAAAISYGTVLGSLAIPVA
                     IVVNVLLIIVGLTKTLNVDIWNIWHAAFVASLVYAVTNDFAMGMLATVIYTMMILLFG
                     DILGPIINKFYGFPNITFPHGTSAPGFVFALPLNWLFDRIPGLKDWHADPETIQKRFG
                     IFGDSTVMGFLIGLVMGILAGYDVAGIGQLAVKTGAVMVLMPKMVALLMEGLTPISES
                     ANSFVQKRFPGRELYIGMDAALSVGHPAVLSSSLILVPITILLAVILPGNTTLPFGDL
                     ATIPFLICLMAAVFGGNIIRTILGGTIYMVTILYITSWVAPFLTKAAQGANFDLQGHS
                     SVTAMAEGGLWTTLLYVGGAKLFGYFALVGIGLIITGLLFYVNRILPKRAAKQTEA"
     gene            3964..4248
                     /locus_tag="IVN13_002653"
     CDS             3964..4248
                     /locus_tag="IVN13_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7013088.1"
                     /translation="MKKLLIMCGTGVATSTVVTGKVKEWLKSKGLENEVKLYQSKVAD
                     EIGKIDDYDIVVSTTVVPDNIKDKVIMGLPLLTGIGIDGLFDEIEAKIKA"
     gene            4275..5102
                     /locus_tag="IVN13_002654"
     CDS             4275..5102
                     /locus_tag="IVN13_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HAP7013089.1"
                     /translation="MLVTTKEMFEKAQKENYAIPAANFFDLDSARTFVEVAEKMQKPL
                     ILAFAQAHIEECPLEEAALIATYLAKKASVPVALHLDHGQDEEIIKQAIELGFTSVMI
                     DASQDEIETNIRRSKAIAEYAHERGVVVEAEIGHVGTGNSYEFKETTDTIYTEVEEAA
                     RFAKETGVDSLAVSIGTAHGQYKGTPKISFERLEEIKNEVSIPLVLHGGSSSGDENLH
                     KCAVSGINKINIFTDFITAAMDVINEEKPEDYFTLKRLANQAIADTLSHYYEVFATK"
     gene            5115..5810
                     /locus_tag="IVN13_002655"
     CDS             5115..5810
                     /locus_tag="IVN13_002655"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP7013090.1"
                     /translation="MRTVRKPFFVVNPKSYLYGEQALKLAKTADQLAAAYDIDIIFTC
                     QHVDVRLIKEQTTQLIVSVQHIDGIVPGRGMGHVLPEGVVAAGAEATFLNHAERPLSV
                     SELCDAVKRAKELGLLTIVCANSLEEAKLVASLKPDVMVCEPTELIGTGQASDISYMI
                     QTNEAVRKMDPEIFVLQAAGISTVADVRKALDSGADATGGTSGIVCAKDPVDTLKAMI
                     EEVSKWKKEQSTK"
     gene            5807..6322
                     /locus_tag="IVN13_002656"
     CDS             5807..6322
                     /locus_tag="IVN13_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016380999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HAP7013091.1"
                     /translation="MITYTEQLVLTSNGQRVTYHNITEQVKEIVAKSGIKNGICLVQS
                     PHTTCSVIFEEFMHDLDFNGDEFLQVDLNRILDRIIPRELTENTDYRYPGPKHLDFLL
                     SLDDPDYAGDAGTILNGDAHIRASFFGASENFIVKEEILMTGTVGSIYFIDFDQNRVR
                     NRKCHILVMGE"
BASE COUNT         2159 a          988 c         1375 g         1977 t
ORIGIN      
        1 gacaaataat atcttaaaaa aattgtcgtg agttttactg acatcaaaaa gtatagggtc
       61 ataataatct gttccacatt aatgtggaac agattacgta ttgatcttta gtgagaaaaa
      121 ctctatgata tagacaagga aaacgttttc tgaggtgatg atgaatgtta tcaaagcgcg
      181 agagtcagtt aattgaatta cttttagagg aggagaattt tcaaccagca agtttcttta
      241 gtgaaaaact caatgtttcg aataaaacaa tttatcaaga tattcagaag ctgggacagt
      301 tcctgaatcg ttttgagtta aaaattgaaa aaaaacctcg catggggatt tacttaattg
      361 gaacgtcaac taaaaaaaat gatgccaaaa tatttatcac tagtaaagaa gctatgtcaa
      421 caaaggtaaa gtatagttca aattatcgta gagtgtactt attcgccaat ctaattttta
      481 aaacggataa aaaaagatat gagtattttg aaaaaaagtt ttttgtcagt attgcaactt
      541 tgcgtaagga ctatgaagaa ataatcaaat attgtcggtt agctgggatt aggattactt
      601 caaatcatgt aatagacaca caaaaaacac ctgaagagtt gatccagcgg acatttgtta
      661 attattttga aaaaaacatc ggtgtaggga atgaaaagtg tcaatatcac tttgtttttg
      721 atcaagcgat ggttaagttt gtggatgatt ttgtaaaaaa tttttaccaa cagtctaagc
      781 agcaaccaac aaattatatt gtccattcac tcaaactttc gttgttgacg ttgattggtc
      841 gagtaaagca tggctaccat tcacaaacaa ataaaaatcc tttgtttgtc gaaataaaga
      901 aaatggaatt atatatggtg gcactagatt tttcaaatgg ggtcaaagat gaattgaatt
      961 gttcctttac gataaatgat ctttacttta tttgttcgtt gtttatggga catggagtga
     1021 gaccgtttgt gaatctggca acccatagtc aacaaatcat ggagaccacc aaacaattca
     1081 ttcaagatat gtcgacactg gttgagaagg atttggggca agatagtcag ctgcttcaat
     1141 ctctatttgc ccatattgag ccaatggttt atcggttgca aatggggatt accattaaaa
     1201 atcctttaaa agaggatatc attcggcaat acagcacaat gtatacgcta gcaacttact
     1261 gtattccaaa attggaaaaa gcaatgggga tcagagtaac ggatgatgaa attacttttt
     1321 tgactattca ttttcagtta gcttttgaaa aaattgtgaa tacgaagcat gtttttctta
     1381 tttgtccaac ggggttggga acttcgcaat tattatttca gcgtattcgt catagttctc
     1441 ctagtaccgt agtctatgaa gtgattgatg ttcaaaagtt gcaggattac aacttagata
     1501 gtgtcgattt gattatttca acagtcaaat tagaaaatga gtatcaaacg ccggttattt
     1561 atgttcgacc attaccaacg aaagaagaaa ttgcattgat taatcgtcat cttcttacct
     1621 tgaatcaaca agacaataca tttttaccat ccattgcacc ggataaaacg attttaaaga
     1681 aatatttgaa ggatggttgg atgtttttga atcaagattt tgatagtaga gaatctgtac
     1741 tttctttcat cactgagaaa tataaagaag aacaattagt aaaggataat tttattgagg
     1801 ttgtacttca aagagaaaaa ctaggagcta ccggactttc atcaggcgta gccatcccac
     1861 atgcaaatcc taaatatgta gtcaaaacaa aaatttctat tatcactcta aaaaacaaaa
     1921 ttacttgggg agatgtggat ataagggttg ttttattctt agcgattgca gaagaagata
     1981 tgattcaagt gaaaaatatg attgcactac tttatgatgt ctttgtctca cctgagagaa
     2041 taaaaagtat tgcaagtaaa gaaacaagga atgagattat taacgaaatt ataggaaaga
     2101 ggaagtaaga tgacgagtta ttttggaaag aaaattgcat tatttcaatc tgaggtaaag
     2161 agtcagaagg aagctttatc aattattgca acttgtctat atcaaacagg tttagtgaag
     2221 gattcgtttg cagagaatgt actgaaaaga gaagaagtgt ttcctactgg cttattaatc
     2281 aatgatgttg ggattgctat accacatacg gatgcagaac acgtgaatga ttctcaaatt
     2341 gcatttatgt cattgaaaac accagtaact ttttatgaaa tgggaactgc agataaggaa
     2401 atttcagtca gcttgatctt catgttggcg ttgaaagaac cacatgcaca gttagaaatg
     2461 ttacagaagt taattacgat gtttcaaaaa aaagaggtgg ttcaaaagct tcgagctgta
     2521 aataatactg aggagtatta tcaaattatt gaaaaagaag gattcgttta ataaccattt
     2581 tgagggggag cattatgtta gatttattac aaaaatttgt tgatctcgga tcaatcgttg
     2641 tattgccaat cttaattttt gtttttggtt tgattttagg aacaaaaccg agtaaggctt
     2701 ttatttcagc attaacggtt ggtgttggat ttgtcgggtt gaatttagtt atcgatttat
     2761 tgaccaatag cttaggacca gcagcacaag cgatggttga acgttttgga ctaaacttaa
     2821 caaccattga cgttggttgg ccggcagcag ctgcgatttc ttatggaacc gttttaggta
     2881 gtttggcgat tccagtagct atcgtagtca atgtgttatt aattattgtt ggcttaacaa
     2941 aaacactgaa tgttgatatt tggaatatct ggcatgcagc atttgttgct tcacttgtct
     3001 acgcagtaac gaatgacttt gcaatgggca tgttggcaac ggttatttat acaatgatga
     3061 ttttattgtt tggcgatatt ttaggaccaa tcatcaataa attctatgga tttccaaata
     3121 ttaccttccc acacggaaca tcagcacctg ggtttgtttt tgccttacct ttaaattggt
     3181 tattcgatag gattccagga ttgaaagatt ggcatgcaga tccagagacg attcaaaaac
     3241 ggtttggcat ctttggtgat tcaacagtca tgggcttttt aattggtttg gttatgggaa
     3301 ttttagctgg ctatgatgtt gcgggaatcg gtcaattagc ggtgaaaact ggtgctgtaa
     3361 tggtcttaat gcctaaaatg gtggcacttt taatggaagg cttaacacct atttcagaat
     3421 cagcaaattc atttgttcaa aaacgtttcc caggaagaga attatatatc ggaatggatg
     3481 ccgcattgtc agtaggacac ccagctgtat tgtcttcttc gttaatctta gtcccaatca
     3541 cgattttatt agcagtgatt ttaccaggta acacaacctt gcctttcggt gatttggcaa
     3601 ctattccatt cttaatttgt ttgatggcag ctgtctttgg tggaaacatt attcgaacaa
     3661 tcttaggtgg gacgatctac atggtcacca ttttatacat cacttcatgg gtggcaccat
     3721 ttttaacaaa agcagcacaa ggggcaaact ttgatttaca aggacattca tcagtaactg
     3781 cgatggcaga aggtggactg tggacaacac ttctatacgt gggtggtgca aaactatttg
     3841 gctactttgc cctagtcgga attggtctga ttattacagg cttactattc tacgtgaatc
     3901 gcattttacc taaaagagca gctaaacaaa ctgaagctta atacaaaata ggagagatga
     3961 cagatgaaaa aattattaat tatgtgcgga acaggtgtgg ctacttcaac agtggtaaca
     4021 ggaaaagtaa aagaatggtt gaaatcaaaa ggactcgaaa atgaagtgaa actatatcaa
     4081 tcaaaagttg ctgatgagat tggcaaaatt gatgactacg atattgtggt cagtacgacc
     4141 gtggttcctg acaacattaa agataaagta attatggggc ttcctttatt aaccggaatt
     4201 ggcattgatg ggttgtttga cgaaattgaa gcgaagatta aagcctaaaa aatacttgaa
     4261 aagaaggtaa tcaaatgtta gttacaacga aagagatgtt tgaaaaagcg caaaaagaaa
     4321 actatgcgat tccggcagca aacttttttg atttagattc tgcaagaacc tttgtagagg
     4381 ttgcagaaaa aatgcaaaag ccattaattt tagcctttgc acaagcgcat attgaagaat
     4441 gtcctcttga agaggcagcg ttaattgcaa catatttagc caaaaaagca agtgttccag
     4501 tagcacttca tttggatcat ggacaagatg aggaaatcat caagcaagca atcgagcttg
     4561 ggttcacttc tgtgatgata gatgcttcgc aggatgagat tgaaacaaat attcgtcgtt
     4621 caaaggcgat tgcggaatat gctcatgaaa gaggcgttgt ggtagaggct gagattggac
     4681 acgtgggaac gggaaatagt tatgaattta aggaaaccac ggacaccatt tataccgaag
     4741 tggaggaagc agcacgtttt gcgaaagaaa ccggcgtcga ttcattggca gtttcgatag
     4801 gtacggctca tgggcaatac aaaggaacac cgaaaatcag ctttgagcgg ttggaagaaa
     4861 ttaaaaatga agtttcaatt ccattagtgt tgcatggggg ctcatcatct ggtgatgaaa
     4921 atctccacaa atgtgctgtt tcagggatca ataaaattaa tatctttact gattttatca
     4981 cagcagcaat ggatgtaatt aacgaagaaa aaccagagga ttattttaca cttaaacgat
     5041 tagcgaacca agcgattgcg gatacgttat cgcattatta cgaagtattt gcaacaaaat
     5101 agaggagggt tctcatgaga acagtaagaa aaccattttt tgtggttaat cctaaatctt
     5161 atttgtatgg tgaacaggca ttgaaactag caaaaacagc ggatcaatta gctgcagcat
     5221 acgacatcga tatcattttt acttgtcaac atgttgatgt tcggctaatt aaagaacaga
     5281 caacgcagtt gattgtgagt gttcaacata tcgatgggat agtccctggt agagggatgg
     5341 gacacgtttt acctgaaggc gttgtagcgg ctggagcaga agcgaccttc cttaatcacg
     5401 cagaacgtcc tttatcggtt agtgagctgt gcgatgccgt caagcgagca aaggaacttg
     5461 gtctattgac tattgtttgt gcaaattctt tagaagaggc taagctcgta gcttccttga
     5521 aacctgatgt gatggtttgt gagcctacgg aattaatagg tacaggtcaa gcaagtgaca
     5581 tttcttatat gattcaaacc aatgaagctg tacgcaagat ggatcctgaa atttttgttc
     5641 tgcaggcagc agggatttca acggtagcag atgttcgcaa agcacttgat tcgggggctg
     5701 atgcaacggg tggaaccagc gggattgttt gtgccaaaga tcctgtggac acattgaaag
     5761 cgatgattga agaagtaagt aaatggaaaa aggagcaatc gacaaaatga taacttatac
     5821 tgagcaactt gttttgactt caaatggaca gcgagtgacg tatcataata ttaccgaaca
     5881 agtaaaagaa attgtggcaa aaagtgggat aaaaaatggc atttgtcttg ttcagtcgcc
     5941 acatactact tgttcagtta tttttgagga attcatgcac gatttagatt ttaatgggga
     6001 tgaattttta caggttgatt taaatcggat tcttgatcga atcattcctc gagaattaac
     6061 ggaaaatacc gattatcgtt atcctggtcc gaagcatctt gatttcttgc tgtccttaga
     6121 tgatccagat tatgctggtg atgctggaac gattttaaat ggagatgcac atatccgagc
     6181 atcattcttc ggagcaagtg aaaatttcat tgtcaaagag gaaatactaa tgacaggaac
     6241 tgtcggctcc atttatttca ttgattttga tcaaaaccgt gttagaaatc gaaaatgcca
     6301 tattttagta atgggtgaat agaaaagtaa taagaaacaa acatttggga aactaattga
     6361 atgatcacac ctataaaaat taatacgaaa gataatagag tcaagtccag actcctgtgt
     6421 aaaatgctat acaatgtttt taccatttct acttatcaaa attgatgtat tttcttgaag
     6481 aataaatcca ttcatcatg
//
LOCUS       DACCKU010000125         6067 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.129, whole genome shotgun sequence.
ACCESSION   DACCKU010000125 DACCKU010000000
VERSION     DACCKU010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.129"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            831..1565
                     /locus_tag="IVN13_002657"
     CDS             831..1565
                     /locus_tag="IVN13_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7013092.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1581..2750
                     /locus_tag="IVN13_002658"
     CDS             1581..2750
                     /locus_tag="IVN13_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7013093.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2766..3761
                     /gene="lacD"
                     /locus_tag="IVN13_002659"
     CDS             2766..3761
                     /gene="lacD"
                     /locus_tag="IVN13_002659"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP7013094.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3840..4766
                     /locus_tag="IVN13_002660"
     CDS             3840..4766
                     /locus_tag="IVN13_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP7013095.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4785..6035
                     /locus_tag="IVN13_002661"
     CDS             4785..6035
                     /locus_tag="IVN13_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7013096.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2026 a          997 c         1246 g         1798 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggatag catacggaaa gaagttctgc accttataaa atttgtcaaa aaaactgttg
      181 ccattccaaa gtttgaaaaa tgctatttcg aggtagaagg atattatata agcacggttg
      241 actaaacgaa gtgataataa aaaaagttat tcaagaataa gaaaaattat gatacgaatt
      301 cagataaact ggagaataca gagaccttta aaagggataa gaaacaacat aaattcctct
      361 taagagagaa cgaaaggaca aagccagcaa cttgatacac gagctagtat tagaggaatt
      421 tattctcata gactcccttt tgaatacgtt tcaactttaa ttttcatgtt cataaatgat
      481 ttgaaaccaa agcaaatgcg ttgcactttt cctccgtctt ccgaaaatcg ttcaattcac
      541 tggtaagtag tcatttgata gtttgaggca aattgttgat tgatgaaggg taggagctaa
      601 aaaaagatac cagttgaaga gagaaaaaga aaaaaataga gggtgttctt gaatgaaaaa
      661 agttggaaaa ttatttttca tttcctttta ttcctattaa agtcagtgat gtcaatcata
      721 ttaacaagac taaaaatgaa aataaaaaaa agattgttct tagtattgac tcataaaaaa
      781 taccactact atacttacat tgtggttata gccagttagg agagacatcg atgcaaaata
      841 cctttcaatc aaaatcactt tattatcagc tggtagaaat attgaaacaa cgaatcgaaa
      901 acagtatggt cgctcatgac aagctgccat cagaaagaga gttgaccagt ttttatggcg
      961 ttagtcgcac gacggtgcga ctagcattgc aagaactgga aaaaatgggc tacatttatc
     1021 gacaacatgg aaaaggaacc tttgtttcgg aattaaggac aaatgcgact gatctagctg
     1081 gcgcatatag ttttaccgaa caaatgcggg caatgggcaa acatcctgaa accaagatat
     1141 tggccttcaa gaagattgaa gcaactaaat atttagcgag tcatctgaat gttcaactag
     1201 gtgagccttt atttcaatta gaccgattgc gcttagctga tgatttgcca atgatgatcg
     1261 agaaatccta tttgccagtg aagctattta tgtccctttc tcaagaaatt ctatttctga
     1321 aacctttata taatttgttt tcagagaatt tcaatcaaat cgtacggttg gctgacgagg
     1381 aattttatgc cagcattgtc cagaaagaag atagtgagtt gctaaatatt ccagaaggat
     1441 caccagtgct ccatttagtt cgctcaacct ataatctcga gaacgaagtg attgaattta
     1501 cattaagtgt agcgcgggct gataaatttc attataagat caggcatctg cgttccacta
     1561 actgaattgg aggaaagaaa atgtttatgt tctcaaaaga agaactacga aagctaggag
     1621 cagaaaatac tacccgagaa attcgacaac aaccggaact gtggcatgag acgtttgacc
     1681 gttatcaaga aacagttgca gatttagatc gtttgtttac ggatgcgaaa caacatgctg
     1741 gagagcgacc aatccgagtc atttttacag gggccggaac ctcgcaatat gtgggagata
     1801 cactaattcc gtttcttact agtcaaggaa atacgaacca gtttattttt caaagtattg
     1861 gttcgaccga tatcgttgct acgccaaaag aatacctgat tcctgatgag acaacgatct
     1921 tggtctcatt tgcccgtagt ggaaatagtc cagaaagtct agccgcagtt aagatggcca
     1981 atcagctggt caacaagatc tatcatatcg tgatcacctg tgcaccagac ggattattgg
     2041 ctacacaaat gcctacaaaa aaagaaaatc tagttttact aatgccaaac aaagccaatg
     2101 acgctggttt tgctatgaca agcagtttca gctgtatgac gctgactgcg ctacttattt
     2161 ttgatcaagc aagcaatcag caaaaagaaa gatatgttca tcaattaagc cgactaggag
     2221 aagaagtcat ccaacgagaa tcagagatcc aagggtatct ggcatcagac tttcatcgca
     2281 tcatttactt aggttcggga agcttaggcg gattgactag agaagcgcaa ttaaaagttc
     2341 tcgagttaac tgctggaaaa atcgcaaccg tatttgatac atctttagga ttcagacatg
     2401 gtcccaaatc ttttgtcaat gagcaaacat tggttttcct gtttgtcagc aatgactcat
     2461 atacgcagaa atacgatgta gacatgctgg aagaaatcaa aaatgacggt attgcccaac
     2521 gcgttgttgg aattggtcaa aagggttcat tgtttagtgg tgattccttt gaattagttg
     2581 cagattcgtt actgccggat ggctatcttg ctttagcaga tatcatggta gcccaaacga
     2641 tcgcattact ttgttcggtg aaggttggta atacaccaga tacaccatcg ccaagtggaa
     2701 cagtcaatcg tgtggtcaaa ggcgtaacga tttatccata tgaaaaatag gaaagagtgt
     2761 gaagcatgtt agaatggtcg gaaaataaaa gacaagcaat ggaacgtcta tcgacgccag
     2821 aaggcattat taccgctttg gcgatcgatc agcgaggcgc gctgaagaaa atgatgtcgg
     2881 ctttagggat ggaagcgaca aaggaaaaaa ttgagcagtt taaaacacta atttcccaag
     2941 aattgactcc ttatgcctca gcaattttat tggatcctga atacggactg cctgctgcac
     3001 agaagcgtgc agcagcgagc ggtctgctgt tggcatatga aaaaactggt tatgatcaaa
     3061 ccgtacccgg ccgcttgccg gatctgctgt cggactgttc agttctgcga ttgaaagagt
     3121 taggtgcgga tgcggtgaaa tttttactgt attacgatta tgaagaagca gaggaaatca
     3181 atcggcaaaa gcaagttttt gttgaacgaa ttggaagcga atgcatggct gaaggactgc
     3241 cattttattt agagttattg tcatatgatg ctaaaaatgt agatacgaaa tcgctttcct
     3301 atgccaaagc aaaaccgcgc aaagtgatcg aaatgatgaa agaattctcg aaaacccagt
     3361 ataaagtaga tgtcttaaag gttgaagtac cagtaaacat gtcatttgtt gaaggaaatg
     3421 caacagggga gatcgcctat acacgtcaag aagctgccgc ttattttaaa gaacaaagcg
     3481 atgcaacaga tttgccgtat atcttcttaa gtgctggggt atcagcggca ttattcatgg
     3541 agacattgcg ttttgccaaa catgctgggg cacgattcaa cggtgtactc tgtggcagag
     3601 caacttggaa agatgcagtc gctcctttcg ttgaaaaagg agaagcggca actttagaat
     3661 ggctgacaga acaagggaca caaaacattc gtcaattgaa tgaagtgata agagaaactg
     3721 ccatcccatg gtatgagaaa gtaagtgaaa gcacttgtta atgtttctaa tatattagta
     3781 gtgataagag atatgtgtga ttataattta tcattattga aactgggagg atattttata
     3841 tgaataaaag attgttagat tattcagctt cagatttttt gaaagctaag ccgatggaat
     3901 taaagcaggc aattttggct tctgaaggta gaacaattat ggcagaaaat gtgccaagta
     3961 gctgtcctta catgggaaaa tcagtgacaa atgcagaatt ggagagagct gctggagctg
     4021 atttaatttt gtttaatgca ctagatcttt ttaatcctca aatttcaggt gtacctttaa
     4081 ctctaaaaga gaatccaata atctggatta aaaaagcttt aggaagagct attggtgtaa
     4141 atttagaacc tgtagacaaa gaagttttga tgagtgaaaa tcgaactgaa atttcaagtg
     4201 gtagaatcgt ttctcctgag actttaaaaa aggcaaatga attaggcttt aattttattt
     4261 gtttaactgg aaatccagga acaggagtat ctaatagaac tattgagaaa gcaataacat
     4321 tatccaaatc aatttttaat ggattaatta tcgctggaaa aatgcatggt gctggtgttg
     4381 atgaaccaat tatgaactta gatatagcaa aaagatttat taaaacaggt gtagatattt
     4441 tattagttcc tgctccatac actgttccgt atttcatgga aagtgatttg cgaggaatcg
     4501 ttgattatat aaaagatttt aatagaaata aatctattga ggaaaaagtt ctagtactaa
     4561 cggctaatgg aacaagccaa gactcttctg accaagaaac tattaaaaaa attgcattag
     4621 catcaaaagc atgtggagct gatattcaac acatagggga ttctttaaat ggtatttgtc
     4681 ttccagaaaa tatttttgct ttgggtcaag caattcgggg atatcgtcat caaattatta
     4741 tgttaggaaa atctaattat cgttaattaa ggaggaaatg aaagatgatc gacaaagttc
     4801 aaaaaatagc tgcaaaaatt agtaatcaaa gacatctact agctttaaga gacagcatgg
     4861 ctgcagttat gccattaatc attattggtt ccttttttat gttattagca aatttcccga
     4921 tagaaggatt tacaaaatgg ttatctgaaa ttggagtaca atcctttttg aacaaagcat
     4981 cagattcaac atttggtatt attggattag ctgttaccta ctctttggga cacaatctag
     5041 caaagactta tggtgtagac tcattatcag caggattatt atctttgagt ggatatgttt
     5101 tacttacacc tttattggat gcagatggtt ctataggatt tccaacaaaa tactttggta
     5161 cctcgggtct ttttgttggt attattgtag cacttgtatc tacagaaatt ttccggtggt
     5221 ttgtacttaa aaatattaca attaaaatgc cggcaagcgt tccaccaaat gtaagccgag
     5281 ctttcgttgc tattattcct ggcttttttg tcgttctact atggtttatt gttgttgtaa
     5341 tttgttataa acttggaatt gaaaatgttc atgctcttat tgcagataca ctcgcaaaac
     5401 cactatcttt actgacgaaa actttaccag gaattatatt ggttatactt attcaatgtt
     5461 tcttttggat gtttggtatt catggtgcac aggtaacggg gccaataatt gaaccgttat
     5521 tattgcaaaa ctctgatgta aatagaattg cttatcaagc tggaaaagaa cttcctaaca
     5581 ttataactta tgaattctta tataactttg ttttttctgg tggtgcaggt tgtttatttg
     5641 cacttgccat tttaacattt ttcttttcaa agagtcagga aaataaaact ttaggtaagt
     5701 tatcaatcgc tccagtaagt tttcaagtag cagagcccct tctatttggg tttccaacta
     5761 tattaaatat aaaaatgtta attccatttg taactgcacc agtagtaaca acattaatta
     5821 cttatttttc aatgagtatg ggattagttg caaaacctgt aggagcaaca atcccatgga
     5881 caactccacc gataattgcc ggatttttag cttctggtgg acgaataagt ggtgcagtga
     5941 ttcaagtaat aacaattgca ataaatgttt tgatatacta tccattcttt aaattagatg
     6001 ataatgccaa gctaaaaagc gaaaaaaatg attagaacta cctatatgtt tctaactgta
     6061 gttaagt
//
LOCUS       DACCKU010000126         5967 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.130, whole genome shotgun sequence.
ACCESSION   DACCKU010000126 DACCKU010000000
VERSION     DACCKU010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.130"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(61..936)
                     /locus_tag="IVN13_002662"
     CDS             complement(61..936)
                     /locus_tag="IVN13_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7013097.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            1084..1416
                     /locus_tag="IVN13_002663"
     CDS             1084..1416
                     /locus_tag="IVN13_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7013098.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            1528..2736
                     /locus_tag="IVN13_002664"
     CDS             1528..2736
                     /locus_tag="IVN13_002664"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP7013099.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            2890..3345
                     /locus_tag="IVN13_002665"
     CDS             2890..3345
                     /locus_tag="IVN13_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013100.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            3707..3982
                     /locus_tag="IVN13_002666"
     CDS             3707..3982
                     /locus_tag="IVN13_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013101.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            3995..>5967
                     /locus_tag="IVN13_002667"
     CDS             3995..>5967
                     /locus_tag="IVN13_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP7013102.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYR"
BASE COUNT         1928 a         1009 c         1269 g         1761 t
ORIGIN      
        1 aaaagacaaa ggtcagctgg atgtggaatc tttcctcata gcctatcttt gtcttttttc
       61 ttatagtttt ttcagtaatc ttgtctgtgg cttcaaataa gtaaagcctt gcccttccac
      121 ttcatgcaca ttgatgattg aaaggaaagc tttttcgtca atctcgtgga cgatcctctt
      181 agcttcagtt aattcactag agctaacgac catatagatg attttttttg aattaccaga
      241 gaatgcgcct tctccagaaa ggaaagtcac acctctttgc agaccggtca tcagtagtgg
      301 tgcaatctct tcgcttttgt tggagacgac aagtagacct tttgcagaat atccgccatc
      361 taagatcgaa tcaatcacac gactgaaaac atacgaaaca atcaacgtat acatcatacg
      421 tttcaagtcg atataggtta atgaagcaag taagacgatg atatcaaatg ccaataggga
      481 acgccccata gtgatgccgt aattttgttc taaaatccga gcgataatat cactgccgcc
      541 tgttgttcca cccatgcggt agacgatccc gcttcctaca cctgcaacta aacccgcaag
      601 tagagaaacg atcagtagat catgctgtaa attgatttct agtggaaccc tttgccagat
      661 ccataaaaag aatgacaaag aaagtgtacc tagaacagta taggccaagg aacgtttacc
      721 gaggatcttt cctcctagta aaatcaatgg gatgttgatt aataaagtcg tataagctgg
      781 atcaatacca aataaagctc ttaagatcaa ggtgatccct gagactcccc cttctgctaa
      841 atgattagcg atgttcaagt aaacaagacc aaatgcataa atacttgttc ctataatgat
      901 aaaaagaact tcttttactg tttttcgttt gaccatatga attccagctt tcaattatgt
      961 attttcacag aatttccatt tagaaattcc ttcctaaatg tagcatggaa attcgatttt
     1021 ctcaattgct cttatctagg atttgcgtta aagttttggt aagatagttt tggagggaaa
     1081 aagatggata aaaagcgttc tttatacagt atgcagcagg aagtggatga atatatccag
     1141 caattcaagg tggggtattt ctctccatta gctcagatgg ctcgattaac tgaagaagta
     1201 ggtgaattgg caagagaagt caaccacact tatggagaaa aaagcaaaaa ggcttctgaa
     1261 ccagttaact cggtagcaga agaacttggt gatgttttat tcgttacgat gatcatggct
     1321 aattcattga atatcgactt aactgaagtt tttgaaaaaa acatggaaaa attcaatcaa
     1381 agagaccata atcgctttga gcgtaaggac gagtaaacaa gatgaagtgg tcctggcctt
     1441 gaagactctg aaacggactt gtcaaacagt caccatgcaa ctgagtcttt atctcaggac
     1501 aagaaacacg caggaaaggt tggaaaaatg aaattaaaac aattacctct ggaataccaa
     1561 aaggcaattc cagttctgaa aaaattagag aatgcaggat atgaagctta ttttgtaggt
     1621 ggaagtgttc gtgatatctt gctgaaccag ccaatccatg atgtggatat cgcaacaagc
     1681 gcatttccag aagaaatcaa acagcttttt ccaaaaacga tagatattgg gatcgaacat
     1741 ggaactgtgt tggtattaga aaatgatgaa caatatgaga tcaccacatt tcggacggaa
     1801 tcaacttatc aggactaccg tagaccagat catgtcgaat tcgttcgttc gttggcagaa
     1861 gatttgaaaa gaagagattt tacgatcaat gcgtttgctt tgaaagaaga tggagaaatc
     1921 atcgatttgt tcgatggtct gcaagatcta gaagaacaga tcttgcgagc tgtaggcaat
     1981 cctcatgagc ggttccatga agatgcctta cgaatgatgc gcgggttgcg ttttgtcagt
     2041 caactagggt tcaaattaga ggaagaaacc ttctacgcca tcaaagaaaa ccacgagcta
     2101 ttaggtaaaa tatctgtaga acgtataaat gtggaattca tcaagatgct cttgggaaat
     2161 gaacgggccg cgggaatcca tgcatttatt gaaacagagt gctacatttg ctgtcccggt
     2221 ttgaaagaag aaggcgaagc acttttacgt tttgctgaat taccatccgt cccattgaaa
     2281 gaagagagcc aagcatgggc attattgatc agccaactgg atctaaaaga aaatgagatt
     2341 cgttcctttt tgaaatcgtg gaagtgttct aaccaaatga ttaaagacgt tcaagcactt
     2401 gtccaaggac tacgacaacg agaaactgga gctttggaac cattcgaatt gtacttctta
     2461 ggcaaaaaac atgcactgca agtagaagaa ttgatgatct attttgacga aacacctcag
     2521 atggatgccg taggagaatc ctatgatcgt ttacctatca agtcattaag cgaattagca
     2581 gtagatggta aaatcttgct agaagaaact ggaaaaaaac ctggtaagtg ggttagtgaa
     2641 gctttgcaat tagctgaaaa agccgtcgta gaacaaagaa taaaaaatga taaaaaacag
     2701 gttttggatt ttttatcgaa agaaaaattg atttaaaaaa cactagactg tatctgctca
     2761 gctttgttgg ctacttaaag tgggcagata cgtcattaga agaaaaaaca taagatgtga
     2821 ccacttacat aaatttgtgc taaactgagg tagtacaaga ataaattaca tcataaagga
     2881 gtctttttaa tgagtaaaga aatgacagcg ctaaaatttt attttagaaa tggagaaacc
     2941 tggacgatca accgccgtca tatcggcgat ctttggatca aacaaatcac aacaagtttt
     3001 ggacgaatca atggcagtga atttgttgaa atccatccgt gtgctggatt taaaatcgaa
     3061 atcttccatg aaggggacgc agttgcgaca catgatatca acctaggcgg actggaaatg
     3121 ggcatgttta atcgcgcatt gaaatacgaa gatatcgagc gaatggaaat cctttaccga
     3181 aatggcacac ctgatcttgt gtacttccca tatctagata aaggaacaga aggattagac
     3241 aatcaatatc aatcaacaaa aatcagcgaa aaaacaggta acttgtatat cgtgatcaat
     3301 ccagatcaac gtgtagaaga tgtatacggg gaatttttcg aataaatcga tttttacgca
     3361 aaaatgaggc tgtgaaagag atgtgtctta ttactactga taggctgtct cttccacacc
     3421 gtttattcag aataagggac tgtgacaaga tttttgtcgc agccccattt ttttgttgtg
     3481 ataaaagaac aagaaaggca ggaatgggaa agtaaacatc cttttgtcag aaacttttat
     3541 ctttcttaag ttttacttga tatctgatac tatttggaat agaatgaggc gttgaaaaat
     3601 ttatggaaat ttctaactag caataaacga tgaaacaatg aaatattttt catccaaaaa
     3661 gaactttgct ataatgatgg aagtttgtgt ataggagatc tggaaaatga gagagagttt
     3721 tgaacaacaa aaaaaactgt tgcatgatcg ctatggcgcc ctttcgatgg atgaccgccg
     3781 tcaaatactt tgtaagttgc gtaaaagaaa tatattgatg tatcgtcagt tggaacgtct
     3841 gaaacatgat ctgcttcgtt tggagtcaaa acgtgtacag tgtgaattag aggggaacca
     3901 aacacaggtt gaagtagtcg aaaccaaaat tttaaagaaa aaagaacaat ttttaaaaat
     3961 gcttacacaa aataaaaaat aaaggggatg gacgatggaa tttgctgagt tagttattgt
     4021 atttgcagta acgatcacct tatcgaacat agctagccgc attttgccaa tgattccagc
     4081 tccactgatc caaattttcc ttggagtggt attaggtctg acagagtggg gacaatcaat
     4141 cgattttgaa cctgaattgt tcttagttat gattattgca ccattgttat ttagagaagg
     4201 agaaaaagca gatatttctt cgattctcaa aaatttcggg acaattttgt tcttggcatt
     4261 tggaggagtc attttgaccc ttgtcggggt aggtgcgacg ttatcattcc tgttgccaag
     4321 tgtcccactt gctgcctgct ttgcttttgg agcagcgtta ggtccgacag atgcggtagc
     4381 agtcagttct ctttctggac gagtgaatat ccctaaaaag gcgatgcata ttttggaagg
     4441 agaaggtctt ttgaatgatg catctggtgt tactgccttc cagtttgctc ttggtgcgct
     4501 gatcaccgga tctttttcag cagtaaacgc ggggatgagt ctagtagttt ctagtattgg
     4561 gggagctttg atcggctttt tgcttgtctg gttcaaacaa aaaataattc atttgatcga
     4621 aaaagcatca gcacaagatg tcacagggta tctgctgatc gaacttttgc ttccttttct
     4681 agcctatgta ttggcagagg catttgatgt atctggaatc attgctgcag tggcagcagg
     4741 tattctccaa gcttctggtt ttaggaaaat ctctgtgttt gatgcagaat tgtcgagctt
     4801 gtcccacagt acatggacaa ctattgcttt tacactgaat gcattagtat ttatattttt
     4861 aggaatcgaa ttgacgcaag tgttctcgcc agtatgggga gacggtttat atccaaacgg
     4921 tcttttatta gcgattatcg tattaatctc tgtcatgctt tttgtgatca gattcatttc
     4981 tatcagtctt ttttatgtat tcaaagatgg cagtaaaaag ttcaaaaaac agttgaacga
     5041 gatcttgatc ttaacatttg gcggtgtaaa gggaacagtc agtttggcta cgatttttat
     5101 tttgccattt tcaatcaata atatgatgtt ttaccagcgg tctttgcttt tgtttctgac
     5161 ggcgggtgtc atcttagtga ctttggtcat agggatcatt gtcttgccta tgctgacgga
     5221 aacagaggaa gcgaaaagca cggatttgaa tgctttgatg attttagaag aggtcgttga
     5281 agttttgcga aaagaaataa aggaaattga tcgtaataca aaagaatttt tagccactga
     5341 agcagtgata gaaaattatc aagaacggat acgtgatctg tacttggaag atttaaccga
     5401 tgatgaaaag caagaagtac aagaaataca agcactgatt ttgtcgatcg aacgggatgg
     5461 attggatgaa agttatcgtt cgggcaagct ttcttctaat ggttatcgtt tttactcccg
     5521 ttttctttct cgattcgaac attctatcac aagccagatt ttgtctttta tcggcttttg
     5581 gttcattgtt gtccgtaggt taatgcgaat cgttctccat ccgaaaatgt tttggcaaag
     5641 aagatatgct gatcgtcaga catttattag tgatgaagat atccaagaaa tcagagagac
     5701 atatcaaaaa aatactcagt tgattattga aagtttagat aacttgactg atatttatga
     5761 tgaaacgatg gttcaatttt ttatccagca acgcaaactt gaaggaataa aaatgaactc
     5821 ggggaatttg atttcttcgt ggatgatcca gcaagatagt ctgtttacga aaaaaatgct
     5881 aagaggttat tatcttgaaa gaaaaatcat tgatgaatac gaagttgctg aaaaaatcac
     5941 gacgttttct gcgaacagct atcgcag
//
LOCUS       DACCKU010000127         5924 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.132, whole genome shotgun sequence.
ACCESSION   DACCKU010000127 DACCKU010000000
VERSION     DACCKU010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.132"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(75..1169)
                     /locus_tag="IVN13_002668"
     CDS             complement(75..1169)
                     /locus_tag="IVN13_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7013103.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="IVN13_002669"
     CDS             1358..2119
                     /locus_tag="IVN13_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7013104.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IVN13_002670"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="IVN13_002670"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP7013105.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="IVN13_002671"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="IVN13_002671"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP7013106.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4772)
                     /locus_tag="IVN13_002672"
     CDS             complement(3759..4772)
                     /locus_tag="IVN13_002672"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP7013107.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4937..5857
                     /locus_tag="IVN13_002673"
     CDS             4937..5857
                     /locus_tag="IVN13_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP7013108.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1180 c         1063 g         1875 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcactaaaa agagcgtggg acaaaagtaa agaacacttt tgtc
//
LOCUS       DACCKU010000128         5916 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.133, whole genome shotgun sequence.
ACCESSION   DACCKU010000128 DACCKU010000000
VERSION     DACCKU010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.133"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..450)
                     /locus_tag="IVN13_002674"
     CDS             complement(<1..450)
                     /locus_tag="IVN13_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP7013109.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IVN13_002675"
     CDS             674..1465
                     /locus_tag="IVN13_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP7013110.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSETANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IVN13_002676"
     CDS             1431..2384
                     /locus_tag="IVN13_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAP7013111.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IVN13_002677"
     CDS             complement(2782..3756)
                     /locus_tag="IVN13_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP7013112.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IVN13_002678"
     CDS             3871..4305
                     /locus_tag="IVN13_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP7013113.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IVN13_002679"
     CDS             complement(4430..5416)
                     /locus_tag="IVN13_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAP7013114.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..>5916
                     /locus_tag="IVN13_002680"
     CDS             5844..>5916
                     /locus_tag="IVN13_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010276791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAP7013115.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARK"
BASE COUNT         1861 a         1117 c         1014 g         1924 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaaccgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaat
//
LOCUS       DACCKU010000129         5877 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.134, whole genome shotgun sequence.
ACCESSION   DACCKU010000129 DACCKU010000000
VERSION     DACCKU010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.134"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(505..1314)
                     /locus_tag="IVN13_002681"
     CDS             complement(505..1314)
                     /locus_tag="IVN13_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7013116.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1314..2060)
                     /locus_tag="IVN13_002682"
     CDS             complement(1314..2060)
                     /locus_tag="IVN13_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP7013117.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2078..2545)
                     /locus_tag="IVN13_002683"
     CDS             complement(2078..2545)
                     /locus_tag="IVN13_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7013118.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2545..2955)
                     /locus_tag="IVN13_002684"
     CDS             complement(2545..2955)
                     /locus_tag="IVN13_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7013119.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2966..3979)
                     /locus_tag="IVN13_002685"
     CDS             complement(2966..3979)
                     /locus_tag="IVN13_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7013120.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4196..4930
                     /locus_tag="IVN13_002686"
     CDS             4196..4930
                     /locus_tag="IVN13_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7013121.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVRNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4914..5612
                     /locus_tag="IVN13_002687"
     CDS             4914..5612
                     /locus_tag="IVN13_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7013122.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQSHS"
BASE COUNT         1902 a         1232 c          978 g         1765 t
ORIGIN      
        1 cgcaaaacat cagcttaaag cgacaaaaac cacgagcagg tccattcatc ctcaaacctc
       61 ggtgatcttc gcggtttaac accatcagaa gaaatgcaca aaaataataa ggagagtctg
      121 agacataaat aaattcccag acttttagtg agtctaaatc cgaataaacg gtgggtcgaa
      181 agcaactcta gctgcgcgtc gatcgcagca atctctaggt gctatgttcg gataaatggt
      241 ggtcaaaagt cagattttcg gccatttcgt aacagccaac cagacaaaaa gcgtctgttt
      301 tgtcttctac tccaattgct caaatcttag cgacttttgg cttggtctta ctctaaacag
      361 tttgtaagcc taaactttat ttgctgtttg tttctaagat caaagcaaaa ctacttttta
      421 gttggttact tttacaagtt ctttttactg ttgaataaat gtaaatctta agaacaaata
      481 tttacttcta taattccaag tttcttaaaa aattccgcca agtgtaccta aaaagcataa
      541 aacaaaaatt cctagaataa ttgtgatcgt tttatgccct ttggagtaaa gccagacgca
      601 taaaaaggta attcctaatg gcagcaagcc cggaaaaatt tgatcaaaaa tactttgtaa
      661 tgaagtttca gctccaccaa ccgtgaattt tagtgacgta tttaaagtaa ccatcgtgcc
      721 tatcatcgcg ccaattaccg ttaaacctaa aatcgaagca ccatgagata atttgtcaat
      781 catatggttg tcttctgtat catcaataaa tttagttcct aaaacatagc catattttgt
      841 cgccaaccat ttaaaaataa aatgaatggc agtatacaaa gtaaaataaa ggatcatgcc
      901 taaaatattc ccttgttgag caaaagaaat acctatgcca gccgcaataa cccgaaaagt
      961 tccccagaaa aatgaatcgc caatacctga taaaggtccc attaatcctg ttttgatttt
     1021 attaattgcg tctgtatcaa attcaggatt tttagcattt tcttcttcca tggtgacgga
     1081 caagctcata acaaaaggcg caatatgcgg tgtaatgtta aatgtttccg tatggcgctg
     1141 caacgctgta taataatcat catcgttcgg atatatttta cgtaaggaac gttcaatacc
     1201 attcatttgt ccatatgcca tcattttatc gaaggaccaa gaggattgca taaaaaagga
     1261 gcgccaaaat atacttctta attctttttt cccaataaca ctttctttta ccattataaa
     1321 tcttcctcct cttcaaaagt tagcttttga acagccagta acgctaagca aagtccgacg
     1381 acagccacac cgacaacatc cattttcaga tacattacgc cgacaaatcc taagaaaaga
     1441 taaggcatca tcgctttatt cgccatcatt ttaattaata acgctagccc aacacaagaa
     1501 agcacaccgg ccgcaacatc aaagctgtcc aatacaacct gaggaatcaa cttgatcatc
     1561 gcattgattg tagacacacc aaaataagta gctacaaaaa ctaaaatggc cgatggaacc
     1621 ccaacgatta aaggttgagc cacataatga tatttttgtg cttttttgaa atcatgattg
     1681 actaatgcct catccatttt gttgttaaaa tacgtaattg gcattgcata ggcgaagtta
     1741 cgccacatct gaagaaagat ggacaaaggt aaagtcaacg caacggcagt tcctactcct
     1801 tgacccgatt gaatcgcaat tgctgtacct aaaacactgg aagcataaac ttcagggggc
     1861 gtcgcactcc ctaccataat tgagcccatg aacatggatt ccaaggtggc accaataata
     1921 attcctgact ctaaatctcc caaaatcaaa ccgacaaaag gcgctgtaaa tagcggtcta
     1981 cccatcatcg atgcaccaaa tgtgtattca tcaatagacg ccaaaaaagc taccagtgcg
     2041 attaatattg cttgtatcat attcttcccc tcctctttta gtcatgcaaa ggattgattt
     2101 ttttgtccgt tggtacagcc tgacattgaa ctgttatccc gttttcagtc atttttttca
     2161 ccaagtccag ttcttgttca ttcaagtaaa cggaactagt aatcattttc cgattctcgt
     2221 ttttctttaa atttcctaaa ttcacttctt taatctcagg tacgtgctga gtaatttcgt
     2281 aggcatcctc caccgtcccg acaattaaaa atacttggta tttcttgatt ttttctccat
     2341 ttaaaacaga gatggcctct gtcatatcct tcaccacaaa ttttgtccct gtaggaacag
     2401 ccatttttag tgtttttttg cgcagattat tggtagcgat ttcattactt gcaacgaaaa
     2461 tacaatttgc acctaagtta tttgtccagg caaaagcaac ttgcccatga acaaaacgtt
     2521 catctactcg taataattta atcattaaaa atcatcctct tctgttttct ctagcaactc
     2581 gttcaccaaa actatccctt cattcccgct attcatagct ttttggatta gttttttcgt
     2641 gtcctgttca cctgataagc aaagctctaa aagcagcggc agatttacac cgctgattaa
     2701 ataaaaattg tctttaccga tttgttcgga gaaagcgtta gacacgcttc ctcccagtaa
     2761 atctaccgtc acaattaatt cgtcatcagg cgctaagtcg gaaaatagct gctctatttc
     2821 ctggttaatg gagtagtcac tatctatata cgcagttatt gctaaaagtg atttttgagg
     2881 accaataata aattctaaag tatcctttaa gcctttagcc atcatggaat gcgtcgcaat
     2941 tacaattttc ctcatagctc ttaccttaat attccacagt ccgataataa cgacgaatat
     3001 ctagtgaatg atcccgcacc ttttcaaaat gagcactcac tcgttctaag gccgttgcca
     3061 taaccattgg cgctacatac tgacgcatag cgcttggtac acctgctaat tcgaaatccg
     3121 cagtgtcaaa aaccgtcaca acatcgctat atttatttac aaaacgctct acgcgatcga
     3181 ctagcggacg cgtttgatct tcacctttta ataacacaaa actcatagat ttatcagtca
     3241 tttcaatagt cccatggaaa aattcggctg catggatcga cttggtggca atccattgca
     3301 tttcttccag tacgcacata gcatattcat aggcagcccc ataagtattc ccagctccaa
     3361 caaacatatg aaattccgta tttttatgtt tttctgcaaa ttcaagtgct ttctgatcat
     3421 tttgttgccg aacacttagt aatacttccg gcattttttg taatgtttga ataaattcat
     3481 catattcagg aaactcaccg ttatttttca tcaggcgtgc acccaaagca aaaaactgta
     3541 actgaagttc ttctactaat gtatcgttgt cagcatcatt ttcaatcgca taatccacga
     3601 tttccccgag tggtgtagca gcatttccta caaacgcaat cgtcgttgca ccttgactat
     3661 tggcatactt aacaacatca acggtttctt gagtcgtacc ggttaatgaa gatgtgatta
     3721 acactgtatc tttattaatc tttttcggct gtgaacaaac cacctctgcg gctatttcat
     3781 gataataatc aagctctgaa tgagtgctta acagataagc tagcgggctg attgtggcat
     3841 atgttccacc agagcctacc aaataaagat ttttaaatcc cttctcactg attgcatctg
     3901 ccacctgatt gattgtctcg atatttttta ctgcaccatt taatactgat agaaaacgtt
     3961 cttcgctaaa acctaacata gttaaaaacc tccaagtcaa taatttgaaa aatatatttg
     4021 caaatgccgg cacattaaca aaccacttaa tcttttttat ataccacctg ttaacgtttt
     4081 taatgtacca cttatagaaa acgctgtcaa tagtttttaa ccacttttat ttttatttgt
     4141 accaaaatcc ttttttcatg tataataaac aaaaatagac tctggagtgt aacgtatgaa
     4201 tactgaagaa ctaacgaaca aattaataca agacattgaa caagaaaaat ataaatacgc
     4261 gcaagctatt ccttcagaac gtgattttgt tcaaatttat gaaatgaatc gttcagccgt
     4321 aaaaaaagcc attcaacaat tagtaaataa agagtaccta ctaaaaatac atggcaaagg
     4381 aacttttgtt aaaaaaaata tccatgaaca tttgcaaatc aaatttcatg gtttgtctga
     4441 attgttggaa cgggaaggga ttcagcctag cagcttaatt ttagcttcag aaataaaacc
     4501 ggctggacac attgtcggga aagctttaca gctggaacca actgaagaag tatttcattt
     4561 ggttcgttta agaagcggcg acggtgagcc aatttctatt gaagacactt atattccatt
     4621 aaatctcata cctgatcttc aagcttttga tttccaaatt ttttcatttt attccacatt
     4681 ggctgcttat cattatcaaa ttgaaaatat ctatcagaca atttccagct caaaagtgcg
     4741 caataaagaa gcccgtttgt taaaaaccag cttaaatcgt ccggtaatca gtcttgctat
     4801 cactgcggtt acgacaaatg aacaagcggt tgaatacact gaagtagtgg ttttgcctga
     4861 attcgccgcc atctatacta aaaatgttca tcaaggaaag gagatgaaaa tctatgccca
     4921 aattgattaa tctgttggct tatgacctga ttgacaaaat tattgcttat attcatgaac
     4981 atcatttaac ccccggaaca aaactgcctt cggaacggga cctatcagaa atgtttaatg
     5041 ttaatcgaac taccttaaga ggcgcgttac aaaaactggt ggacaacggc accttacggg
     5101 cgcaaaccaa tagcggctat tatttaactg cgccaaaatt cgaaaaaaat gcccatcatt
     5161 tctttacacc gatagaagat tctctgctta gtaagcagcg gctacagcta aaggcaacgg
     5221 ctctttcacc gaaaaaagca gcagctcttt gtccggaact gaatgatttc aaacagcagc
     5281 aaccacaagc ttatttagaa tatattgatg cagaagcaat tagcttgatg ttattaatcc
     5341 ctatggaaac attaaagaaa agtaatttct cggacaatcc tttccaagat tttaaaagtt
     5401 tggcatatac gcgttctcaa gaaatttctt taataactga caatgacgtt gcggcggact
     5461 tgctgggagt tgacaaagaa gaggttctgt tatttgtacg agaggaaata agagccggta
     5521 aggagttggc ggcgattgct ttaggttata ctattagtcg ccgctgcaac atacaggtta
     5581 atgttgctac gaccttgcaa tcccatagtt aggaaaaact attcgctgta agttcaaccg
     5641 tttcaatgca agcgaatcac aaatcaaaaa gtatttaact cgtgtttatt agaaatagtc
     5701 atgaactttt agtctccaag atcaaaccga cagcgttcaa ctctgagaag caaaagtaaa
     5761 tgtacgatca agtatgaggc tgggacagaa gcgctaagcg gttctgttgc aaagttttaa
     5821 atctactatc agataaggta gaataataga aaaagatagc aggaggaatg acgatga
//
LOCUS       DACCKU010000130         5789 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.135, whole genome shotgun sequence.
ACCESSION   DACCKU010000130 DACCKU010000000
VERSION     DACCKU010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.135"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(145..543)
                     /locus_tag="IVN13_002688"
     CDS             complement(145..543)
                     /locus_tag="IVN13_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP7013123.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(540..1313)
                     /locus_tag="IVN13_002689"
     CDS             complement(540..1313)
                     /locus_tag="IVN13_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7013124.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMVKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1310..1993)
                     /locus_tag="IVN13_002690"
     CDS             complement(1310..1993)
                     /locus_tag="IVN13_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP7013125.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2160..3047)
                     /locus_tag="IVN13_002691"
     CDS             complement(2160..3047)
                     /locus_tag="IVN13_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP7013126.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKITIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYDA
                     AAAITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3044..3913)
                     /locus_tag="IVN13_002692"
     CDS             complement(3044..3913)
                     /locus_tag="IVN13_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP7013127.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4050..5336)
                     /locus_tag="IVN13_002693"
     CDS             complement(4050..5336)
                     /locus_tag="IVN13_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7013128.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTGWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5609..>5789
                     /locus_tag="IVN13_002694"
     CDS             5609..>5789
                     /locus_tag="IVN13_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP7013129.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMP"
BASE COUNT         1797 a         1270 c          967 g         1755 t
ORIGIN      
        1 ttgaaaagaa agtctgatgc ttagagtaga atttcataaa aatttactat ttcataagta
       61 ttcacgagaa aaatacaaac aaaaaagcgg aaataacgct catccgcctt cttgtttgtg
      121 tttatttcct cttcaaacgc ttgtctatga ttcagacttt tctctatcgt tattttcatc
      181 taaaagttct tgcattccga taatttctcg atattgatga atcttagtga taccaatgac
      241 tttgtctaca tcaatactaa aacaaatacc attacctgga atatcgatgt tgatgccttg
      301 accaatcgca tccattactt tattcgtcaa actatcgggt actaatgtca acacaatatc
      361 tttttctggc gaaatgtcca aacctaaaaa tttccctgaa tcatgtacac cagaacctct
      421 tccatgtaag atcgttccgc ctgctgcacc tgcttctttt gaaaagtcga taacatcttc
      481 gcccattcct ccgtcaacga tcgtcacaat catgtgaaga ttcaatgcta caagatcttt
      541 catttgcttt cccccaattt ttgcaatctt tctttttcga ttctgtcttt ttcatctttt
      601 cgtttataaa tatatcctag tactaaagtg gataatattg gtgtcaatgc aatcattgca
      661 accacaccaa acccgtcaat gatcggatct cttccttcaa tttgtgcagc gattgccacg
      721 ctcattgata aaataaatgt cgaacacatc gggcccgttg ctactccacc gttatcaaaa
      781 gccattgcta aaaagatctt gtccactttt cgtcctaaaa taaatgctag cccgtatcct
      841 gggatcaaga aataataaag agagaatccg acgagcaaac gccacataga caatcctact
      901 gctgcgccga tcccaattga aattaccatc agcatgattt tcgatcggat tctcccctcg
      961 ctcaattctt caacctgttt gaccattaca tgaatcgccg gctcagcaaa ccctactaag
     1021 aaccccatta aaaagccaag gggaaccagt atataattgt aaggcaatcc cgcaatagca
     1081 ctgcctaaat gctgtcctac cggtacataa gccatattga ctccgtgcag gaaaaggatc
     1141 aacccaatag tcgttatcaa gaaacctttc ataatgtcaa agaaacgctt tttgcgcata
     1201 cgaaaactaa tgatattcat taaaacaaat aggattacga taggtgccac tgccaaaatg
     1261 acttccatgg taacgatttc gattccatct atcacttgct gccagaaact cattggaaga
     1321 tcacccccaa aatcatcacg gcaagaatcg gccctaacga agcaataccg accattccaa
     1381 aactgtcatt ttcatttttc ttttgtttga tcatactagt catccctccg gcaagggcca
     1441 agataaatgg aacagttact ggaccagtag tcactccccc cgaatcaaaa gcaatcggca
     1501 taaattcgga actggtgaaa aatgaggcga tcaaaacgcc tatgtagcct atagccatca
     1561 attgataata ggatagcttg aatacgaccc gaagcagcgc aaaggctaga aatactccag
     1621 tgccgacgct gacgatccca atcagcaaca ctctgccaat ctctccgtca gatatatcat
     1681 atacttgttg tcccaaaact tgcacggaag gttcagcaat cgtgatcacg atcccgatag
     1741 caaatcccaa actgaccaaa acagtcaaac ttttcttttt gatcatatat ttccctacta
     1801 gatctccgac ttccatcata gaataatctg ctccaaacaa aaagagcgtc attccgatca
     1861 tcatgattgc cgcaccaacc agaaatgaag cgaggtcttc tccttctaac ggtgcaaaaa
     1921 caaacgttaa aatgacaatc aggacagtca tcggcaatat agcagcaatc acttctttaa
     1981 aattttttag catgtatcct cctcctttac tctttaagtt taccttaaaa tgaaagagtt
     2041 ttcgagcaca aacttaaaac gttatcattt aatttttctc aacataaaat aacacacgtc
     2101 ccaaaagtat ctttttactt ggaaaaatga ctttgaagga acgcgcttat tgtgtttttt
     2161 tattttacag agccgatcat tccttgaacg aattgctttt gcaaaacaaa aaatacgatg
     2221 gcagttggta aagtagcgat gactaatcca gccatgatca ttccatagtc tggtgtatag
     2281 gaagcaccca tagcagataa aacaagcgga accgtccgtt tatcaggaga ttgcaaagct
     2341 actaagggcc ataaataatt gttccagcta ctcatgaatg tgatggctgc agccgcgtca
     2401 taggtatttt tagccgtcgg catatacact ctaaaaaaaa tacttagttc ttttagaccg
     2461 tctaatcgag cggcttctac tagatctttt ggaaaagatt taacattttg tcgaaagaag
     2521 aagatcaaaa aagcagtgct aacagatggc aggatgacaa ccaagaaact gttgattccc
     2581 aaaaccgttc cgttcaattg tgaaaacatt cgatataaag ggatcatcaa tgccgcaaaa
     2641 ggaaccatca tcgatagcag tagaatattg aaaattaggt cttttcgttt actttgataa
     2701 atctcaaatc catatcctgc cattgacgaa atcagcaatg caaacacagt ggtcaccaaa
     2761 gcgatcatcg ctgaattcca aagggaacga gtaaaattca gctcattgga aaacaaattt
     2821 tgaaaattga tgatcaagtt actgccaatc gttatttttc ccgatgtgat atccacgggt
     2881 gtattcgtca ttcccaaaac catccagaca aaagggaaaa tggaaataaa tgataggatg
     2941 cttaatgctg tatactttaa tactgaccca attgtcgaac gaagttctaa tgcttgtttt
     3001 ttgacgttgg ggcgttcgat tgtttctttt tctaccatat ttttcatcga ctttctcctc
     3061 ctactttcgt ttggattatc gacaatatca caataaagga aacaataaca aaggaaactg
     3121 ctgccgcata tccaaaatta ggcgtgaatt tataactcaa attatagatg tattgagata
     3181 gggtggtcgt tgcatttgct ggtcccccgc cagtaatatt ttgtacttca tcaaaaagct
     3241 gcaatgtccc gatcgtcgaa gtaatggaag taaacaaaat gattggtttt aaatttggga
     3301 tcgtcacttt gataaactgc tgccatttag aagcaccgtc aatagatgct gcttcataaa
     3361 tctccgaatc aatattttgc atgcctgaaa ggaaaaaaat catattgtat ccagtccaac
     3421 gccaagtgat cgaaagaatg atcagaactc tcgcccaaat tggatgagtc aaccagccaa
     3481 tcggtgtgtc aatcatacca atgcccaaca acaagttgtt tacaaggcca gattgagaaa
     3541 atagactttt catgatcaga gaataactga ccatcgaagt gatacatgga agaaagatag
     3601 cggttcgaaa caacccttta tatttcaact ttttatcatt taaaaggttg gaaataaaaa
     3661 gtgctagaaa caacatgatc ggtacttgta caagtaaata caggaatgta ttaaaaaaag
     3721 cttttttaaa agttgcatct gtcaacatcc gctgataatt acctaacccg ttaaatgtca
     3781 tattgacagg gggaccactt tgaaaagacg tgatcaaagc caaaaccatc ggaataaaaa
     3841 ccatgattga tatcaaaatg actggcaaga ttaaaaaagc atgcccgttt ttgaataaat
     3901 ctttctgctt cattcttttc cctccaattc tatcattgat tatctacatg aaaagacaag
     3961 aaagacaata tctgtaaggc agacgtgatc ttttttgtct tttcgtttgt ctgatgaaaa
     4021 agcgaagaaa ttcacttttt taccaccgat caattagcca attgtgcttc tacttgtttt
     4081 tgtgtatctg ctagtacctt gtctgtctct tctccattga tgattcgatg aagagcttca
     4141 gctacgactg cttcaattgc ataggtctca tgtccgtaat tcacttctgg tatttcagat
     4201 gtccatgttg aaaaatcgtc aaagatcttt tgaccactgt aaaattcaga agcttcttga
     4261 taagcttctt gttcattcgc gctaagcatc gtagatacta gtccgatttc tttcgcaaga
     4321 gtgcccatca gttcttcatt cgaagcaaac gttgcttcta ggaaatcttt cgctgaatct
     4381 tctccagcta ctcctttgat cacataccag ccagcgccgc caaggttaga agcttgcgct
     4441 ttttgcagat tttctggaag tgcaggtatt ggtgcaatct tccattttcc agattggttt
     4501 tctgctcctt ggatcgttga agaataccaa gcgccagttg ggctagaagc tactgctcct
     4561 gattgtatcg cagttactcc agcattccag tcagaggttt gttcgactag atcttctttc
     4621 aaaagagtgg caaacaattc aagaccatac tttaaagatg cattgtcttg gatcgtgact
     4681 gtctccccat cttcagatgt ataccattct cctgcctgtt gcataatcat ccttactcga
     4741 ccaaggtcag aaggattgac ttcagcgatt ttctttcccg tcttttcttt gacatctcgc
     4801 gctacttgaa tatactcgtc ccacttcaga ttctccatat cctcttctgt ataacctgct
     4861 tctgccatca gatccgtacg atagaagttt gctgttacgc ccgaatcgaa aggaacaccg
     4921 tatattttat ctcctacttt gtttaccgca aatttatacg gtgcaaaatg atcttccttt
     4981 acaatagcag acaaattagc gaatgcatct ggataagaag tcaaatagcc ttgtatccta
     5041 taatcttcaa tcaagacgat attgggcaat ccttctgtat tcccgctcgc taaagcggtg
     5101 ttcagtttct gaacgatatc atcctgagac atcgtaacaa cttctatctc tgtttcttcg
     5161 ttctcataaa ctttttttgc ttcattcact gctttgatat tgaatgtctc atcccacgcc
     5221 cagccagtga ctttttcttc tttgtctgct gtactactag cacttccgca tcctgttaag
     5281 aaacctgatg ctgccgctaa acaaacgact ccagcaatta actttctgat gttcattttt
     5341 atccgctcct tttccatcca ttagttgttt acgtttgcta cattcatcat accaaaattt
     5401 gaaaacggtt tctttattat tcagatactt caaaaatttc tcctaaaata ataaaaaaat
     5461 acaaatcaac tatgacaaaa ggaataatct tagcatctag accaaagatc gaaatggtga
     5521 ggtttcttta aaataaaaat atccttgcat tttctcctag aaataagtat ttttaaatta
     5581 caagactatt cttaaggcgg gtaaaaaaat gccaatacgc gtaattctta tcgatgatga
     5641 acagacgatt ttagatggaa tgaaacagtt gatcaattgg tctgagcacg ggtttcaatt
     5701 aatagggact ttttccagtc cgttgaaagc gcttgatttt tctttggtac atgcaatcga
     5761 tatcgtggtc acagatgtaa tgatgcctg
//
LOCUS       DACCKU010000131         5676 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.136, whole genome shotgun sequence.
ACCESSION   DACCKU010000131 DACCKU010000000
VERSION     DACCKU010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.136"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            239..406
                     /locus_tag="IVN13_002695"
     CDS             239..406
                     /locus_tag="IVN13_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7013130.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            432..776
                     /locus_tag="IVN13_002696"
     CDS             432..776
                     /locus_tag="IVN13_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP7013131.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            781..1062
                     /locus_tag="IVN13_002697"
     CDS             781..1062
                     /locus_tag="IVN13_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013132.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            1165..1479
                     /locus_tag="IVN13_002698"
     CDS             1165..1479
                     /locus_tag="IVN13_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP7013133.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1457..3151
                     /locus_tag="IVN13_002699"
     CDS             1457..3151
                     /locus_tag="IVN13_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP7013134.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3171..4349
                     /locus_tag="IVN13_002700"
     CDS             3171..4349
                     /locus_tag="IVN13_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP7013135.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4312..4998
                     /locus_tag="IVN13_002701"
     CDS             4312..4998
                     /locus_tag="IVN13_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP7013136.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSAGTEAKKEQSVEEILNDVEEKRAEKIAAFTAAL
                     NKTFGQGDAK"
     gene            4998..>5676
                     /locus_tag="IVN13_002702"
     CDS             4998..>5676
                     /locus_tag="IVN13_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP7013137.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVNNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKTAE"
BASE COUNT         2052 a          803 c         1212 g         1609 t
ORIGIN      
        1 cactgtggcg gaagtagaag acgcagcggt aaatggcgag tagcctcgtg agagcctggt
       61 aagttctcgt gagtggtgca atcccactcc agcgacttta agcaaccgag gcatcggcgg
      121 tttaaaaata taggggtgcg caatttcgta cgcgttttgt gcatcgtgca agttactatt
      181 acatattaga tcactctttg agtggtcttt ttatttttgc acaaaggagg aaacaacaat
      241 gtatagacca caatacttag aacagaagta tgaagtaatc actgttcata aaggtaatgg
      301 cgaaaaagta tatgagtata gaagaccaat aaagagcgat acatataaac gaaaggaaaa
      361 caatgaagtt attccattgt atggcaaaag aatagctaag cattaaataa gattgcgaaa
      421 ggagacggaa catgaccgag gaattctata gatggctatt acagttgaca agagaagatc
      481 gtttggttaa gttctatcag tctcctaaat ggcgcaggct tagagagaaa gcgatgaaac
      541 gagatcacta tgaatgccaa gagtgtagaa gactaggtaa gtatcataga gtagagaacg
      601 ttcatcatat aaaggaagtc aaggatagac ctgacttagc tttagattta gataatctta
      661 tttgtttatg tgttgaacat cataatgaag ttcatggcag atatcttaca gcattagata
      721 aacaagagaa gaagatagaa agctttgcta acttcgatgc aagtgaaagg tggtaagtgc
      781 atgatcatca atgataatgg cagagagtat gatacagaaa agattgaaga gtattcatct
      841 tatactcagg gattaattaa acgtttgata tacgttcgct atgtaggtat tagggatctg
      901 ttatcagata actgttgtag taaatacaaa gtgaatcaag taagagaagc gttgaataaa
      961 gataataacg tcgaaagaat aaaaaatgtt tttggatata gtattgaaga gattaattat
     1021 tacattgact tcgctgaagc tttcattccg atggtgagat aaccccccct taaaataaat
     1081 cgcaaatttt ttgggggtga tgaaacggag ggggctgtca ggaaaagaga ttttttcgaa
     1141 ctttatcatg aaaggagggc taaaatgttt aaaaacgaat tgtctcaaaa tcgctacaga
     1201 gaaaaattac gccgctcttt aataagccaa ttggaaagtc agaaaacaaa tattgagcca
     1261 ttcttagata atgttgatcg ttatatcagt ttatgggaaa cggcgatatc actggaagaa
     1321 gatatatccg agaacggcat tagactggag aatggtaaaa agaatgaatc agtagcgttg
     1381 cttgtttctg tcaacaaaca aatgggattg atgttggata aacttgccat tactcctgaa
     1441 ttggtaggtg aagcaaatga atcaattcct gagttataag catattgaaa attggttcaa
     1501 agctatagaa gaaggcacta tcaaggtatg caaagagcaa ttattgctaa aaaattatct
     1561 agaagaaaga gtctttacta gagaagatat ttacttcgat aagcagatgg tagaggattc
     1621 aatcaatata ccagcacaat actttccatt cgaattaatt ccgtgggaaa aatttctaca
     1681 atgttttatt tatggtgttc gatggaaaaa agataaaaca ctagtgttca atagatatct
     1741 ttcattaatg ggacgtggta atggtaaaac tggttttgct tcttggaaca acttctttct
     1801 actaaccgct aaacacggta ttaaaaatta tgatattgat atctatgcca ataatgaaag
     1861 ccaagcaaag actagttttg atgatgtatt taaagtaatt aaagatcatc ctgatttaga
     1921 taaaaaagta tttaaagcta cgaaggaagt tattcaaaat atcgctacaa acagcaaact
     1981 tcgttataac acggcaaatg ctagaacaaa agatgggaag cgaccaggtg caaaccgctt
     2041 tgatgaaatt cacgaaaatg aagattattc aatgataaat gtggctactt ctggtggtgg
     2101 taaaattcga gattatagag aattttatga tacaactaat ggtcatgttc gtggtggtcc
     2161 gcttgatgac attatagaag aatcaaaaat gattctttct ggagaacttg gaattgacaa
     2221 ggatggagca gaattttcta gtttgtttcc atttatttgt cgcttggata acgataatga
     2281 agttgatgat cccgacatgt gggaaaaagc ttgtccaact attaattaca atgcagattt
     2341 aaaacggaaa atgtttcaag aatactctca aatgcaacgt aatgctggtt taagacttac
     2401 gttcatgacc aaacgaatga acagacctat ggaagataca cgatttgctg ttgcttcata
     2461 tgatgatgtt ctgcatacga aagaaaaaga atttcctgaa aaaatggatg aagtgatagg
     2521 aacagtcgat tttgctgata gacgagattt tgccagcgtt gggttgctag gaaaatacga
     2581 taaagatgtt tattttacac aacatacttt tatccacgaa tcagcccttc gattacaaaa
     2641 catcaaacga gaggttatag atatttctat agatcaagga aaatcacaga tcgttcatgg
     2701 aaaaaatata gaagctgatt atattgtagg ttggtttctt gaaatgagta ataaatatta
     2761 tattaaaaaa atcgctatgg atatgtaccg tgcaaaaata ttgaagcccg ctttagaaga
     2821 agcaggtttt actgtggaaa ttgttcgaag cggatctgtt acacatggta tgttaaaaga
     2881 tctggttgat gaccttttta ttaatcaacg tttatttttt ggtgacgatg cgattatgcg
     2941 ttggtattgc atgaatgtat atgaagagca tatttctaat ggaaatatac gctatgaaaa
     3001 aatagaacct gaaactagaa aaacggatgg ctttttttca ttccttcatg gtttgaattt
     3061 tttagatgat atttatgatt ctgctcctgt aacagtcaca aatagctcag tagaaaatac
     3121 aggaactgga tttactcctc tagtattcta acttgaaagg aggtgagaaa gtggggattt
     3181 ttcaaaaggc ggtaggatac ttcacaaaaa aagcaacggt tcctttagaa gaatactttt
     3241 gtaaattgca agttgatttt gtgtatcgaa aatttgcgat tgaaacttgt attgatttga
     3301 ttgcaaatgc gatgagtaaa gcggaattca agtcatatga agatggaaaa aataaaaaga
     3361 atgatcttta ctataggctg aatgtagctc ctaataagaa aaataatgca acagaattta
     3421 gaaaaaaact gatcaggaga ttaatattct acaatgaagt attgatcgtt tctccgtcta
     3481 ataattctag cgaaatattt attgcggata gttgggatgt cacagaatat gcattgaaag
     3541 atgatgtgtt ttctcaagtg caaattaaca acatagtcct tgatagagaa tttctagaaa
     3601 gtgatgttat ctatataaaa tacgcagatc aacaaattag gcaactagtc gatgcgtatt
     3661 atcaagcgta tgggaaactc atttctagtg ccatgaatgt ttacaagcgc tctaacgctc
     3721 gtagatacgt actgaaaggg aatttattcc gaccgcaaga caatacaaca caagatcaaa
     3781 tcaataaaat gatgacatca caatttaagg cttttatgga agctgataat gcaggtgcgg
     3841 tatttcaatt acaaaatgag tacacattag aagatttcag cggaaacttc caaagcaatt
     3901 caagagatat aaaaaactta atagacgaca tctttgagat gacagcagca gcgtttcacg
     3961 ttccgaaaaa cctactaaag ggagacatga gtgggttatc ggatcaagtg gacgcttttt
     4021 taatgttcga aatcataccg attgctgaac ttattcagga tgcgtttaac gctagtctct
     4081 atgaagtaga agaatacttg tcagggaatt ttgtacgtgt ggatacaact atgatcaaga
     4141 ttactagctt caaagatttg gttgacgcta ttgatgtagg cattagaaat ggagtattta
     4201 caatcaacga aggaagagaa cgcgttggaa atgatcgctc tgataaggcg atggcagatg
     4261 aaatatttat aactaaaaac aatcaacaag tatcgaaagg aggtgaggcg aatgacgaca
     4321 atgaaaacat ttctagcagt aaagaatgaa ggcgcagtac cgcaaatttt tattcaggga
     4381 tttattggtt ctagttggtt ctttgaaggg aatactgaca agggaatcaa aaatattttg
     4441 gatagtctag gtgatcaaga agaaattgaa gtagtaatta attcaaacgg tggagacgta
     4501 tttcaaggga ttgctattgg gaacttactt aagtcaaata aagcaaaagt taacgttgtg
     4561 attaacggct tagccgctag tgctgcttca attatcgcaa tggctggcga tactataaaa
     4621 atctacaaca atgcacaatt gatgattcac cgcgcttcca catacggaga aggaaatgtc
     4681 gatgacttcc gtacgattgc tgaccaactg gaatcaattg ataaatcggt aaaggcttca
     4741 tataaaacac gattcaatgg cacagatgaa gcattgcaag aacttcttga aaaagaatcg
     4801 tttatggatg cagaaacagc tttgagttat ggattggtcg atgaaattat cgatgcagaa
     4861 aatagcgcag gtaccgaagc taagaaagaa caaagcgtcg aagaaatttt gaatgacgtt
     4921 gaagaaaaaa gagcagaaaa aattgctgca tttacagcag cattaaataa aacatttgga
     4981 caaggagatg caaaataatg acagttaaaa atttaaaagg tgtaacagct gcaagcgacc
     5041 aattgatgaa agcttttaaa gatggtaacg aagagtcttt tagcgcagct atggtaagtt
     5101 tatctaagga aatacaggat aaaattttag aagaagcaac agcaaaaaat caagatcaat
     5161 tagtattaat gaaccgtggg cagcgtgtac taactacaca agaaacaaaa ttctataacg
     5221 aagtagtgaa taatgaaggt tttgcaggag ttgaagaatt agtaccagct actgtatttg
     5281 aacgcgtatt tgaagatcta gaacaatctc atccactatt gcaaaaaatt acttttgtta
     5341 acacaactgg tgtaacagaa tggatcgtgt cacgtggagt caatccagca tggtggggca
     5401 aactttgcga agctgttaaa aaagttttag ataatggctt tgacgtaatt aacatgaagc
     5461 agttcaagct atcaggttat attcctgtat gtaaggcaat gcttgactta ggtccagtat
     5521 ggttagatcg ttatgttcgt actgttttag ttgaatcatt gagaattgca ttagaacaag
     5581 caattgttga tggtactggt aaagatatgc cagtcggaat gatgcgcgac atgagcaaac
     5641 aaactagcgg agaatatgct gaaaaaacag cagaac
//
LOCUS       DACCKU010000132         5636 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.137, whole genome shotgun sequence.
ACCESSION   DACCKU010000132 DACCKU010000000
VERSION     DACCKU010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.137"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(80..334)
                     /locus_tag="IVN13_002703"
     CDS             complement(80..334)
                     /locus_tag="IVN13_002703"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7013138.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(445..699)
                     /locus_tag="IVN13_002704"
     CDS             complement(445..699)
                     /locus_tag="IVN13_002704"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7013139.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(892..1167)
                     /locus_tag="IVN13_002705"
     CDS             complement(892..1167)
                     /locus_tag="IVN13_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP7013140.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1139..2092)
                     /locus_tag="IVN13_002706"
     CDS             complement(1139..2092)
                     /locus_tag="IVN13_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7013141.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2704..4197
                     /locus_tag="IVN13_002707"
     CDS             2704..4197
                     /locus_tag="IVN13_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP7013142.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4332..4628
                     /locus_tag="IVN13_002708"
     CDS             4332..4628
                     /locus_tag="IVN13_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP7013143.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            complement(4652..5611)
                     /locus_tag="IVN13_002709"
     CDS             complement(4652..5611)
                     /locus_tag="IVN13_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7013144.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDESFIQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT         1804 a          962 c          976 g         1894 t
ORIGIN      
        1 gaattcttgt gaaaaagttc ttcgtgttct cttagacata aaaattcctc ctggtatgtt
       61 ttcttctagt ctacacacct taatttttct gtctagttaa ttgtagccta tccatgatcg
      121 gtttattaga cctttgaggt ctatattttt taactacaat cgaccttaaa tttaattgtt
      181 tcattagctt ttggacatgt ttaagagata ctgaaatccc ttcttttaat aaaacttgat
      241 ggatctttgt ggcaccatag cgttgtttat tcgtaaaaaa aattgttgaa atcttctttt
      301 tcaacttatt atttctgatt tctgtagctg tcaacggttt attcttataa aaataataga
      361 cacttctagg gatttctaat aaccgacaca acaaagaaac agtgtaatcc ttacaccatt
      421 tttcaataaa ttgaacaagc gaatttaggt tttccgagag aatatggctg cggctttttt
      481 taagatttca agttcctcat ttaaacgagc attttctttt ctcagttcag ctacttcttt
      541 tccagtcagt cctgtggact gatcaggtaa atataaattc ttccatttgt aaatcgttgc
      601 ttcagataag ccatattctt tcgctaaccc tcgaacagaa cgtccagttt gattcagttc
      661 aacaatcatt tgtttaaaat tttcttcata tcgggtcata aaaattcatc ctctcgtaga
      721 taagtttaat ttattcttgt ctctacgaaa agctgtctaa gatttgatac taacaccaaa
      781 tgttgtatac ccccaaacaa tacacatatt ttgaaggatt aggattaata aagctaaaaa
      841 tatagtaagt atactaaata tatttactat atctagtata cttactatat tttaatcttt
      901 tgatcgtcta ttatatataa tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat
      961 tgcaagtttg actatttcat acataggaat atcttttgca taactaagat cccgaatggt
     1021 ttttagtacg ggaggatcta cttgaactgg ttttctttct ttcgcagtgt actttcgttc
     1081 ccctaactcg acattaatat catctttaat tgaaatatct tcagcaccaa ttttcttctt
     1141 atctttgttc gttggtaata atgccattta gtttaatctc ctttccaaga ggtaggatat
     1201 gattggccat tgagtcagca tatttaggtg tataatcaaa tccttcaata tcaccatgtt
     1261 gttcatagta ttcaattctt tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc
     1321 atcttcgatc tacataatca tttaattgga taccattctc accatacatt tctaaccgat
     1381 ccgagccttt aacaatgctt ttaaaaatgt ttgattcacc atacttatca attgtttttt
     1441 gatagtttgc gtgttgagag gaacgtcgca ctgagaaaag aacaggcaaa attccaatga
     1501 tttgaagatg agactcttca aaattcgtga caatcggaac taaaacttta ttaataaaat
     1561 cttctgttcc ttccattgcg taccgtttta attcttgcat tgcaataata taatctgcag
     1621 cagttaagaa tgcacgaacc acattatctg ttgatggtgg gcagtcgata aagatataat
     1681 cgaaattttt tctcaattct tctaaaggtt taatgaaggc caatgatctc ttacgacgat
     1741 caggatattt ttcggacaag tattcattaa gttcttcaga tccaggagag ccagccataa
     1801 tatataaatg tggacttact tgagtaatac ctgaaaccca gtcccctttt ttgattgctg
     1861 cagacatgga tcgatcaaag ctagtaattt gatacgttga tccaatgttg gaagtagtat
     1921 ttctttgttg gtcataatca attagcaaca cgtctttttc aaaaagttga ctagaaacaa
     1981 tcgctaataa atcattgtct gtagtcttac caatgccgcc ttttctacct gtaagcatta
     2041 caacttttcc tagtttatct gatgttttaa atgcagtccg taagttttcc atagttccct
     2101 ccaatttcct atactaaata tatttagtat acttcatata cttaatatat tcgatatatc
     2161 taatatactc caaattgata agaattcaga ctttataagg aatatagtgg atatactaaa
     2221 tatatttgct atattcagta tatcagatat attcaaaatg tcaaaatata tttgtgcgtt
     2281 ttgcgttgac aatttagagt gaaaagaggt aaacttaatt ctatagaaac cacagataat
     2341 tcactattta caaagtagca aaagcagtgg tactctttaa acagaaaaat tacataaaaa
     2401 aaagagacgg aaataattga ttttagcaga atcaattatt tctgaatcac atggttagga
     2461 catgtgttca cgctcgtctc aagaatatgt aagtagctac caactactta tattaaaagt
     2521 tgatcggata ccttttccga ttgcctttat tctaccaaaa aaaggcgagc aactcaacag
     2581 aagaacgttt atttagtgta cttaaattaa atattcttaa acactgaaga gagaacacat
     2641 actatggctg agattaaagc taaactgtgt tctctttttg tttgagaaag gacgttacaa
     2701 tcgatgttga gcgaaattta ttcagctatt cttaaaaacg gcttacgtac atacaaatat
     2761 cgaaacagca ccatgaaacc tgcaggtcat attgattcca gtaggagtgg tgctatcttt
     2821 gcgtaccgta gcaagcaatt aatgaatgtt gggcgtggta tggtcattac ttcggaagaa
     2881 gctattttag agaacgaaaa gaaactaacg cattggacac ctaataccta tcgctttggt
     2941 acttatgcgg atgattatcg aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa
     3001 atcaacacat ttgtagttga tatcgattca aaagagaacc atcaaggcga aattatttta
     3061 gcctgtttag atcaagtggg ctacatgcca acgctaattt tagaatcaga tcatggttat
     3121 caagtttatt ttgtactaaa gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg
     3181 atcgatgtag caaaaaaaat ttcaaaaaca attcgtctac agttggctga aaaattgtcc
     3241 ggagtggatc taggatgtaa ccacttcgga attgctcgat tcccacaaga aaataatatt
     3301 gtttttaaag aaatgaacta tcagtattct tttgctgact ggattcaatg gtcgatgaag
     3361 cttgaatcag atcaaaaaac aaaagaagat cgagaacgaa aactaattgt ctttcctgag
     3421 aaaaaagaaa ttcgtcaagt agatgaaccg tggttcgata tgttacttca taaagcaaac
     3481 atcgtcggcg gagaaggacg tcttggtcga aataatgtga ttttcacgct ttctttagct
     3541 tattactctt caggttatgc attggatact tgcgaataca atatgttcac attcaacgaa
     3601 cggttacagg aaccgctatc tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct
     3661 ggtaattatc aagcagcaag cagagaattc attattgaat tgtgccagca atgggtttca
     3721 ccaaccttaa aaaataatga tctgttcatt caacgacgtg gctggtggaa atttaaaaag
     3781 ccacgagatc aaagaaaata tagtcataaa aatgaatgga aagaagatct attggcttat
     3841 ctaagcgaaa aaagtcttgc tagtgagcct tatttgaacc tgtcaaaaaa agaattggct
     3901 tcacaactaa cgatgcccaa gcgaacactt gataagatct taaatgagtt acgagaggag
     3961 aataaagtct tctacaaggt aacacgagga agaaatggcg gaatgatcat tgcttcagtc
     4021 aaaacgttgt tcgcgagggt tattcaacta agtaaagaaa agagactaga ttattcaaca
     4081 aggatcacag aggtatttgg actcaacgag agcttagtag aagagacatt tagacaatta
     4141 tcaaccccct tgaaccctct taaagaagtg aatctttttg aagtagatac aggttgaaaa
     4201 ataaaaggtg caaattgcct tatacaatat atctatgata cttctgtagg tgttgctccc
     4261 tttgcctagc tacgtgtagg tggtatactt ataactgcgt atgcagtctt atgtatcatg
     4321 gaggaagttg tatgtcgtta aaaaattatg tctattcaca tcctgttaat gtgtttatca
     4381 ttggtcgttt gggattatct gtggaagtgt tctgtgagct ttatggtttt aagcagggga
     4441 ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc tttgccgatt gagtttcttc
     4501 atgggttgtc cttagcttct ggggagacta tggatcaggt atatgattgt ttgtgtgtgt
     4561 tagagcagga gtatattgag tataaaatag ctaacgagtt aagaaaaagg aagaagtata
     4621 tccagtagta gtttttattt actcttaatt gtcaaattaa gttagacaaa tcatcaatac
     4681 ttacgtaact caaaaatact tccaaaggtg ttcgataatt cagtgatttt ctaggaatat
     4741 tatttcgttt cgatgcgaca gattgaataa aagattcatc tactgaattg aaatccatag
     4801 atttcaataa accatctcta cgtaacaatc cgttagaatt ctcgtttagg cctctttgag
     4861 acggcgttcc tggatcagcg aaataaatgg caatatcatt ggcattactg atctgtttcc
     4921 aatttgaaaa ttcctttcca caatcaaaag tgatggattt gaatagattt ttcggtacag
     4981 attcaaacca atgatttaat ttgttttcaa tatcaatcgc ttgtctacca cacggtttca
     5041 atgtgatgat aacttttgat aatcgctcaa ctaaggtaat tacagcactt ttatgtttca
     5101 aacctacgat agtgtcacct tcaaggtgac caaactcatt tgagaattgg ctataatctt
     5161 tttcacgttc atgaatagag cgacggaaag cttgttttcc acgtctttct tgatgtccat
     5221 ttggtttacg cttgcctttc atcggtaagt cagaaggatt aaatagcccc tttttgaaca
     5281 tacgataaat ggtacgagca gaacaacgaa taggaaacgc tgcacgacca acaatcacat
     5341 cgggtgtcca tccttgaaca acctttcttt gaatatattc tgattgttcc tcaggtaaaa
     5401 caagcgggcg tctaccacag tttgatttgt ttttcttata ctgttgatag tactctaagg
     5461 ctgattttcc ttgcttgaag aacaggtata ctttatggat cgtttgtctt gaacgattca
     5521 agaaatgcgc agttttcgca acagattgat ttattttgaa ataagactct atcattacaa
     5581 gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa ttcttt
//
LOCUS       DACCKU010000133         5409 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.138, whole genome shotgun sequence.
ACCESSION   DACCKU010000133 DACCKU010000000
VERSION     DACCKU010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.138"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            175..792
                     /locus_tag="IVN13_002710"
     CDS             175..792
                     /locus_tag="IVN13_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7013145.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            833..1132
                     /locus_tag="IVN13_002711"
     CDS             833..1132
                     /locus_tag="IVN13_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013146.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            1166..1333
                     /locus_tag="IVN13_002712"
     CDS             1166..1333
                     /locus_tag="IVN13_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7013147.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(1378..1983)
                     /locus_tag="IVN13_002713"
     CDS             complement(1378..1983)
                     /locus_tag="IVN13_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7013148.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            2108..5095
                     /locus_tag="IVN13_002714"
     CDS             2108..5095
                     /locus_tag="IVN13_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAP7013149.1"
                     /translation="MDEIKSNKKAFGIYLGEPSTEQLNQFFYLHEKDLELMETIRRPA
                     TRLGFALQLVSLRFLGTFILDLTNIPQNVLSYVATQLNIDPGEIIYYSRRQTRYQHTK
                     LIKSHYGYQDFNQPEIETYLANWLLNRALYTTESSDMLFDMLLKKCLDEKIVLPGITT
                     FSRFVASAIEKAEEHLYRQLSLVPTEDERKRLLNHLELIGTPVYGAIIKMDILRTPLA
                     DDSHKEIARGFDRLKQFQAFSTEKWKMESIPEGKIKVLANYAFKAKAQLIQRMSEQKK
                     LALLVAFIFIYKRKAMDEQILALSNFFETVFRRAKNKESKERLRTIKDLDRAASTLSD
                     IVDLVMSSHSDTNELSLRDRILEKYSEENVTEAVSQVRLLVKNDREPIAISELLNTYR
                     KFRKFIPDILTTLTFDSNDYGNNCKELWTFINNRFPKPITYKMFGAIKHTLPKKWIYY
                     IHEYPEQTNKCVLIAAVELLIQSLKRHDIYIPQSVLYNNPMDCLIDEKTWVQQKYVLL
                     EQLDLPSSANEMSLWLKNDLELSYKEALNRWPTSKMARIENADRLVVSRLKKTYEKKE
                     DKSFRRRIQQLLPKIDLSDLLLEVNQQLNLTQSFNHLSDKDTKMKDLDISLLAVLLSE
                     SCNIGFSPVSKEGFDSLKYDRLVYVNHHYIRVDTLSGANQKIIHAHRKLATSKIWGDG
                     HMASADGIRYITPQRSLYSRSNPKYFGRGRGITFYNFVSDQYIGFHGMVVSGTLRDSL
                     YLLEGFLNQPSELTPNQIMTDTAGYSDMIFGLFGLLGFQFSPRIANDKGTKLWRIDAN
                     AHYGELDTLSQNSINMELIYTHWEDILRVAGSLKSGKVNATELTKSLQRNGQPTPLGK
                     AITEYGKVYKTKHQLRYISDEIYARQILEQLNKGEARHALCRNIFYGRKGKLYQTYYD
                     GMEEQLNALGLVTNAIIYWNSLYLEKVFEQMKAEGFDCSEEMIRRQSPLISDHINFVG
                     KYTFRYEAELSNGKLRPLNVEEF"
     gene            5260..5409
                     /locus_tag="IVN13_002715"
                     /pseudo
     CDS             5260..5409
                     /locus_tag="IVN13_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
BASE COUNT         1826 a          907 c          996 g         1680 t
ORIGIN      
        1 aaatataatc atgcgggagt gtaaacttac tgtttcaaaa agtatacttt ttgacatgcc
       61 ttgattttct ttgtttatgt atggcataat aaccacataa tacaatttga aacgtttctg
      121 atttaatctg attgttttga aacaatttta gtgaaaaaag ggtagataaa aacgatgaga
      181 aaattttttt atatccgggt cagtagttat gatcaatcga tcgaacgaca actgatttcc
      241 gcgaaggaat taggaatccc tgaagaatat atatttgtag aaaaagccag cggcaaagat
      301 tttaaacgcc ctgaatatca attaatgaaa cggatgtttc gaaaaggaga tttactctat
      361 ctacaatcat tagatagact aggcagaaat aaacaaatga ttttagaaga atggcgagaa
      421 ttaactaagg aaaagcagat tgatatttct gtgttagaca tgcctctgtt agatactgct
      481 agttacaagc aactagatgg gttagagacg cttatctctg acttaattct gcaattactc
      541 agctatatgg ccgaagacga gcggaaacga attaatagcc ggcaaagaga aggcgtagaa
      601 gctgcgaaac aaaagggtgt aaaattcggc agaaagaaaa ttgctttacc tcctgaattt
      661 cctgcgatct attctgactg gaaagcagga aagataactg cagtctatgc tatggatcaa
      721 ttgggcatga aacgaaatac cttttatcga cgggtgaaag aatatgaaca taccttgcac
      781 cacttttctt gatagatata acagctatta gacgggaagg gagagtttga aaatggactt
      841 aacgaatcaa agggtcgttt ttactggaac gctgcagcaa atgacacgaa cccaagccat
      901 cggtttggtt cactctttaa acggccattg tcaatcaagt gttacttcaa aaacaaattt
      961 cctggtttgt ggtcagtatt ctaagtcgtt gtttgatgat tctctctata caaaaaagga
     1021 acaaaccgcg cgggacttga ttgcgttagg tcatgagatt accattttat cagaagtcga
     1081 attttatgcg ttaattagtc aacagttgaa agaatggcaa cggtatttat aatcgttagt
     1141 aaaatttaca aggaggaaaa tttttatgaa gcgaaaatct tatgatcgtt ggggcattga
     1201 aataactagt tttaatcgaa atattgcttt tcacgtggag tgtgactgtg gggaattagc
     1261 aaaaagtgtt tatcaaaaaa attactttta ctgtcctaaa tgccatcgcc gatacggggt
     1321 acatggaaca tagaaacgaa atagtgcggt agaaaagcga atgaatgtag tatatttcta
     1381 caaatcattc gctttcttta ttttataaat agttggtcga gagatcccgt tttcttttgc
     1441 aatctctgca actggaatgc cttgctttaa catgtccacc acggttttaa aaactaagcg
     1501 tttttgcgga tctgttgagt taatagaata ctcgattggt cgtcccttat atcggccggc
     1561 tttttgagcc aataatactc cttgccgctg acgttcacga attttctcac gctcgttttg
     1621 tgcaatataa cttagcagtg ataaaaacat atcgaacata gcggtatcca ggagttcatt
     1681 tccagtatta aaatctaaaa actgtgcatc taatattttt aacgctactt ttttctgttt
     1741 cataaaagca actgtatttt ttatatcttc ataatctctt cctaaacgat ctagtgatgt
     1801 aacgatgacg caatcacctt cacggatata atttagtaat ttttgaaact caggacgctc
     1861 agtatcttta ccactggctt tttcttggaa taatttatca atgttaagtc cttccatcat
     1921 tgccaattgt cgatccaaat tttgatcttg tgaagatact cgaatatagc caatttttcc
     1981 cataaaaaac tcctttttaa aagtgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgctatcat agcattttta aaaataaatt ttatgtagac ttaaaattta cttcatggag
     2101 ggtttaaatg gatgaaatca aaagtaacaa aaaagcattt ggcatttatt taggagaacc
     2161 tagtacagaa caattaaacc aattttttta tttgcatgaa aaagatttag aattgatgga
     2221 gactatacga cgaccagcta ctagattagg ttttgcacta cagttagtct cactacgttt
     2281 tttaggaact tttatattag atcttaccaa tattccccaa aatgtattga gttatgtagc
     2341 aactcaacta aatattgatc ctggagaaat tatctactat agcaggagac agaccaggta
     2401 tcagcacaca aaattgataa aatcacatta tggatatcaa gatttcaacc aaccggaaat
     2461 agaaacatac ctagctaact ggcttttaaa tagagcgctg tatacaacag aatcttcaga
     2521 tatgttgttt gatatgcttc taaaaaaatg tctggatgaa aagattgttt tacctggaat
     2581 tactactttt tctcgttttg ttgctagtgc aattgaaaaa gctgaagagc atttataccg
     2641 acaattgtct ttggttccaa ctgaagatga gagaaagcgg cttctcaatc atttagaatt
     2701 aattggaact ccagtttatg gggcaataat caaaatggac attttgagaa ccccgttagc
     2761 tgacgatagt cataaagaaa tagccagagg ttttgaccga ctaaagcaat ttcaagcttt
     2821 ctctactgaa aaatggaaga tggaatctat tcctgaaggg aaaataaaag ttcttgcaaa
     2881 ttatgcattt aaagctaaag ctcaattaat tcaacgaatg agtgaacaga aaaaactggc
     2941 gttgttagtg gcatttattt tcatatacaa acgtaaggct atggatgaac aaattttagc
     3001 actatctaat ttttttgaaa ctgtttttag acgcgccaaa aataaggaat ctaaagaacg
     3061 tcttcgtacc ataaaagatc tagatcgagc cgcatcgaca ctttcagata ttgtagattt
     3121 agtaatgtcc agtcattctg atactaatga actatccctt cgcgatcgta tcttagaaaa
     3181 atattctgag gaaaatgtta ccgaagctgt atcgcaagtt cggctattgg ttaaaaatga
     3241 tcgtgaacca atagccattt ctgaacttct gaacacttat cgaaagttca gaaaatttat
     3301 tccggatatt ttaactacac taacatttga cagcaatgac tacggtaaca actgtaagga
     3361 actctggacc ttcattaata atagatttcc taagccgatt acttacaaaa tgtttggagc
     3421 tattaaacat actcttccaa agaaatggat ttattacatt catgaatacc ctgaacaaac
     3481 taataaatgt gtattgattg cagctgtcga attgttaatt caatctttaa aacgccatga
     3541 tatttatatt cctcagagtg tcttatataa caatcctatg gattgcttga ttgatgagaa
     3601 aacttgggtt caacaaaaat acgttttatt agaacaacta gatcttcctt cttcagcaaa
     3661 tgaaatgtct ttatggttaa agaacgattt ggaactgtct tataaagaag cgctaaatcg
     3721 ttggcctact tctaaaatgg ccagaataga aaatgcagat cgcctggttg tctctagact
     3781 taaaaagaca tatgaaaaaa aggaagataa gtcttttaga cgtcgcattc agcagttact
     3841 tcctaaaatt gatttgtcag atttgttatt ggaagtaaac caacagttaa atcttactca
     3901 atcatttaac cacttaagtg acaaggacac caaaatgaaa gacttggata ttagtttatt
     3961 agcagttctt ttatcagagt cctgcaatat tggtttttcc ccggtttcaa aagaaggatt
     4021 tgattcttta aagtatgatc gtcttgtcta tgtcaatcat cattatattc gagtggatac
     4081 actttcagga gctaatcaaa aaataataca tgcacacagg aaacttgcca cctctaagat
     4141 atggggtgat gggcatatgg cttcagctga cggtattcgt tatatcacac cacaacgttc
     4201 cctatattct agaagtaatc caaaatattt tggccgtggt cgtgggatca cattctacaa
     4261 ttttgtctct gatcaatata tagggttcca cggaatggtc gtttcaggga cactgcgaga
     4321 ttcgctttac cttctagaag gattcctcaa tcaacccagc gaattaactc ctaatcagat
     4381 catgactgat acagctggtt atagcgatat gatttttggt ctctttggct tattagggtt
     4441 tcaatttagt ccaagaattg ccaatgataa aggaaccaaa ctttggcgaa tcgacgctaa
     4501 cgctcactac ggggaacttg atacgttgtc tcaaaattca attaatatgg aactgattta
     4561 tacccattgg gaggacattc ttagggttgc aggttcgttg aaatctggaa aagtgaatgc
     4621 aacagaatta acgaaatctc ttcaacggaa tgggcaaccc acgcctttag gaaaagcaat
     4681 aactgagtat ggaaaagttt ataagacgaa acatcagctt cgttatattt ctgacgaaat
     4741 atatgcacgg caaattttag aacaattgaa taagggggaa gctcggcatg ccttatgccg
     4801 taatattttt tatggacgaa aaggaaaact ctaccagact tattatgatg ggatggaaga
     4861 gcagctcaat gcattaggat tagttactaa tgccatcata tattggaact cactgtattt
     4921 agaaaaagta tttgaacaaa tgaaagctga aggattcgac tgttccgaag aaatgatacg
     4981 caggcaatct ccactaatta gtgatcacat caattttgtt ggtaagtata cgtttagata
     5041 cgaagctgaa ttatccaacg gtaagttacg tccattgaat gtagaagaat tctagcaatg
     5101 ttcgggtttt gcatgctacc gcactatttc gtatgactgt tccagcgaat cacttcgaat
     5161 agaaaaagag aaaaacacga gaacagcgtt aaatcaacgt ttcttgtgtt tttacttttg
     5221 tgctaccgca ctatttcgtt tctatgttcc atgtacccca aaatcggtaa aagagaaggc
     5281 aatcaagaga actttattac gaatatcaaa aaattcattg ttcatttact ggaagctgta
     5341 cctaacgata tagaaaaact aaatttttct gattaccagg aacagaaaga aaaagaagca
     5401 gaaaaaagt
//
LOCUS       DACCKU010000134         5244 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.139, whole genome shotgun sequence.
ACCESSION   DACCKU010000134 DACCKU010000000
VERSION     DACCKU010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.139"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1..526)
                     /locus_tag="IVN13_002716"
                     /pseudo
     CDS             complement(1..526)
                     /locus_tag="IVN13_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            547..1747
                     /locus_tag="IVN13_002717"
                     /pseudo
     CDS             547..1747
                     /locus_tag="IVN13_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit R"
     gene            2481..3449
                     /locus_tag="IVN13_002718"
     CDS             2481..3449
                     /locus_tag="IVN13_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP7013150.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            3460..4275
                     /locus_tag="IVN13_002719"
     CDS             3460..4275
                     /locus_tag="IVN13_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP7013151.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKMGNEFAGKSCLVDGSFGFGIHYD"
     gene            4343..5053
                     /locus_tag="IVN13_002720"
     CDS             4343..5053
                     /locus_tag="IVN13_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP7013152.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYVMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKQMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            5089..>5244
                     /locus_tag="IVN13_002721"
     CDS             5089..>5244
                     /locus_tag="IVN13_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP7013153.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYD"
BASE COUNT         1780 a          793 c         1044 g         1627 t
ORIGIN      
        1 ccgtatctcg tttaacgaaa taatagcgta ataattgaca aaaatgatac gcagttcgca
       61 attcttcgtt gtagcttaac agctcattaa cgatttctgt ttgactaatt ggtcgtttaa
      121 acagtcgatg ataatgccgt ttgagtgcct cccatgtcgt ggaatctttg agtaagagtt
      181 tccaatatcg cttgagccga cggtacttct tctgattttc agagtgtgag gttcgaaaac
      241 gattcatgat ctggacacgt aattgattga agctgcgatt gatgtgttgg acaatatgaa
      301 agcggtcagt tacgatctcc gcatttggaa agaccgtttt aagcaactga caatagctgg
      361 cattcatgtc catcactagg tacttgacct ttaaacgggc ctttctagga aagcgcagga
      421 agtattgagt tagtttgtaa agtctgcgat cttcaagggt cgtaaatagc tgattggttg
      481 tcccatcaac acaaataaaa ctcatggaac cagaagtggc aacattgtgt acttttcttt
      541 gcaatcttgt gtacaaaact attgcaaaac acagcgcata agaatcctta caaaacagta
      601 gatgaagcaa cccaattaga tcttgttata gttgttaatc agctcttaac gggttacgat
      661 tcaaaatggg tcaacaccct atacttagat aagtcacttg aatatcagaa cataattcag
      721 gctttttcac gcacaaatcg tcttaatggt cctgaaaaac cgttcggaac cattcgttat
      781 tatcgtaaag cacatacgat ggaacagaac attgaagagg ctattgagtt atactcaggc
      841 aaacaagcaa aagggttatt tgttgaaaaa ttatataata acttagaaaa gttgaacgat
      901 atattcgaag aaattaagtc cgtatttaat atttctggca tagaaaactt cagtcattta
      961 ccggaggttc caggggaaat taagcaattt gtaaatcttt ataatcaact aatccattgt
     1021 tatgaatcag cgatgataca gggattcaca tgggcaaaat cgacttatga aatccaggat
     1081 tatgaaccta taacagtagt aatcacagaa tatgaattta atatcttggc acaacgttac
     1141 agcgagatag agagaaccta gagacggaaa tgatagtata gcgtatgaca ttgattacca
     1201 attacctgaa ggtagtcgta gtcgtattaa ttatgagtat ttaaacacaa acttcaaaaa
     1261 atacatgaat ataaaagaag aacgtgattc gaatttaatt caatcattgt taaacgactt
     1321 acatcgcaca tttgcgcaat taccagtgga gttacagcca tatgctgaga tgattctaaa
     1381 tgatgtaaat aatggggaat tagttatcaa agaaggatgg gaatttactg attacctcaa
     1441 tgaatatcag cttagtgaag aggacgattt tatccaagat ttagtggatt caaccaatat
     1501 taatgaggca ttattacgtg agatgttgga tttacgctta accgaggtaa acattaatga
     1561 atatagtcga tttgataaat taaaatcaag tgttaacgta ggtcatttca gtggatatat
     1621 cgagaaaaat ttaggtaaaa tggtaatacc aatcaaggta aatatgctaa ttgacagatt
     1681 attgcgtgca tttttattag aagatgtatt cgatgttaca gagtacataa agaattactt
     1741 taactagagt acacctacat cttatagtac ataaaaaaat tcatcttttt tagtttaata
     1801 attcaccaaa aaggtgagta atgccatttt tgaaaattga gcttgtttta tgtctacatc
     1861 tgtatccgta tataatccaa aggattatca actcagaaga gtctttattt tttctgaata
     1921 taactactgt atcaaaaaca atcgattatg agacaaaaca aaatgacctc ttttatttaa
     1981 gaaaggaggt cattttttgt atcataaatt gtattaaaat caatgtatca ctttttaatc
     2041 agttttaaga ccataaattg cgtactaaaa gaaattctgc aattcaaaat aaagcatttt
     2101 aagccatttt caaatttaag tgcacccatc tacccgtcaa atcactttat tttccactcc
     2161 agtagtttcc taattcaact gtgtgcaaaa acgatttctt ctaaacaaat caagacaaac
     2221 ttataaacct tcatcccagc accataatta tttaaatcat aggcattttt cgaaattagt
     2281 atatgtactc aagtttcact gcagattatt tatatttttt agagaaataa atttttccta
     2341 ttgacaaaca caaagaaagc ggatacaatg caattatgtc acatgacgtt atatgacgtt
     2401 atgtaatatc tttacattta atcagttact tttattttgg ataaaattaa catttttaaa
     2461 attttaagga ggaaatgttg atggatgtag caaaaacggt taacgcaatt gtagaaaaaa
     2521 atgatggaga gatttctgga atttatttgg tggcttgtgg cggttcgctg gtagatatgt
     2581 atgtctcaga ttattttctt aaagccgaag catcgaagtg tattacaggc ttatatacag
     2641 cgaacgaatt taaccatgta accccaaagc aattagatga taaatctgtt gtcattctat
     2701 gttctcatgg aggaaataca gcagaaacgg taaaagcagg agaagtcgct caagctagag
     2761 gagctgtgac tgttggtctg actcataata aaactgcgga tttattaaag gtttctgatc
     2821 atagtttcct ttacgaatgg ggagatgatg caaaaatagt caataatcct atggccatca
     2881 ttcttgattt aactgtatct ttggtcaatg ttaccgaaac atttgaacat tacggtaaat
     2941 tcagagaagg catgaaggtt atcgataccg taattgaaaa tggcattaag caggtgcagg
     3001 atcgtattaa agtgtttgct gataagtaca aagatgaaac aatgttttat atcttaggta
     3061 gtggaccatc atatggacat gcttatggtt tttcaatttg ctctttgatg gaaatgcaat
     3121 ggcttgattc aacttctgtt cattctggag aattcttcca tggacctttt gaagtaacag
     3181 acaaagatac gaattttatt ctcttgatga gcattggacg cactcgtgct ttagatgaac
     3241 gggcaaaacg attccttgat aaatatgcag aaagagtaga aatcgttgat gcaaaggaac
     3301 taggattgga tatgatatca gatgaagttt ctgaattctt caatcccatt ttattctaca
     3361 gtattctttc agaatatcgt tcagctttag cagatgtacg caatcatgat ttagatgttc
     3421 gtcgctatat ggggaaagtg gagtactagg aggattaaaa tgaaggtatt aggcttaggt
     3481 gataacgtcg tcgataaata tgaaaatctg ggaattatgt atcctggagg aaattcattg
     3541 aattttgcag tttttgcaaa aaaactgggg catgaaagta gctttatggg agtttttggc
     3601 tcagatgagg aggcagctca tgttttatca gcaataaaag aaataggact ggacaacagt
     3661 cactctcgct ttgaaaaagg cgagaatggc tgtgccagag tgaagattga tgagggcgac
     3721 cggattttcc ttggcagcaa ttcgggaggt gttactcgtg aatacccgat tcagcttact
     3781 gaagaagaca gagaatattt aaaccagttt gaattgattc atatgggatt atatagccat
     3841 gtcaatcatc tattagaaga gttacaaaat gtttctggaa agatttctta tgatttttct
     3901 gacgacttta cggaagaaga ggttcagcgg gcgattgata aagttgattt tggcttcttt
     3961 tctatagaag acttttccga tgaagaagta aagcagtttt tgatgaagca tttttgtgag
     4021 cgaaatgatg tattgattgc aacccgagga gaaaaaacgg ctattggtta tgatggagca
     4081 aagttttatg aagtgaaccc cgtgctagaa gaaccagttg atacaatggc agcgggagat
     4141 tctttcttaa cagctttcct actttatttt ctcgaagggg acgatatagt caccgcgatg
     4201 aaaatgggga atgaatttgc tgggaagtct tgtctggttg acggttcgtt cggctttggt
     4261 attcattacg attgagcttg ttttaaattc ctgcatttga taacctaaag aaagaaatcc
     4321 cggtaaagtg gaggttaaaa aaatgctgga tacaaatgca caattaccat tgtacttaca
     4381 attaaaagaa atattaaaaa ataaaatcaa tacaggggag tatcccgaga atacgaaaat
     4441 tcccgctgaa tcagagctga tagaaagtta tgaggtcagc cgaatcacag tgagaaaagc
     4501 agtagaagaa ctggtagaag aagggtacct acttaaaaaa caagggaaag gaacttttgt
     4561 caaccggcat aaagtttttc gtaaaattga atatgttatg ggtttcagtg agagttgtaa
     4621 atctaatggt tttacaccga ctagcattgt tttggaacgc aagatcattc cagcaactga
     4681 agggatggca aaaaaactcg atatcgctca aggtgatgat gttttatata tccagagaaa
     4741 gcaaatggcg gataacatcc ccattatgtt agaaaacaat tatttttctt atgaaagata
     4801 tagtttctta atggaggaag agctgactga ttctttgtat gaagtactta aagtccaagg
     4861 taccttagcc atcaatcctg gggaaactgc acttgaaatt gtcacagctg atgaccaaat
     4921 tgccaaaata atggaagtag ctgttgggac gccctttttc tttatggata ctttaatcaa
     4981 cgatcaaaac aacataccaa ttcatgtagg taatcaatat tacctgggag aagtatatcg
     5041 tttttactta tgatttcctg ttttgatttc aaggagtaga ttttatttat gaagcaaata
     5101 ttgcggtcaa aaatgatttt tgatggagaa cagccacctt tcaaaggtta tattgtaatc
     5161 aatgaaggga tcattgaaaa ggtggaaaac aactggaatt acagtgatct attaaatgaa
     5221 aacacggaat taattgctta tgat
//
LOCUS       DACCKU010000135         5217 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.140, whole genome shotgun sequence.
ACCESSION   DACCKU010000135 DACCKU010000000
VERSION     DACCKU010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.140"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..108
                     /locus_tag="IVN13_002722"
     CDS             <1..108
                     /locus_tag="IVN13_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7013154.1"
                     /translation="VIVPKTGSTHLVTILAVSLLLLVLATFGYAVLKFN"
     gene            complement(144..2201)
                     /locus_tag="IVN13_002723"
     CDS             complement(144..2201)
                     /locus_tag="IVN13_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP7013155.1"
                     /translation="MKLLNPKTVIAIGTFAATVALAGSADAASTNVMYRLYNPNNHEH
                     FYTSSTKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVTSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNTLDSAYKGLKKKPAPTVNKTELQALYNQVKSTAKGDFTEGSWNNFQTAL
                     SNAKKVLDDSKASQTQVNNAKNSLDSAFKGLQHKPKPSEYAVTVRHMNRQTGTLLKEE
                     HVSVKAGTSYTAKARTDLTNFEAPQYQVNGNETQTQTINTNTTFTFYYDEVFLVEVKA
                     RPNKVSDTDPNIQELYNYSKSYPVVYGQSITIEAPMFTNFVLDPRESSSNTVTLNNVT
                     SNQGINFAYTHQYNVTVNHINVDTNAVLSTETQTVYEGDSFSTSWKNMTDQNYFLCRN
                     DDSSVTVDKNGQRVINNVDQNRTITFKYKNISLSDLNNYVRQKELAWLNSYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENINSFYLYVD
                     EILSDTGAVNAMVMWKDSAAHNANLLYNNQSIVGLSHAYEVSADGSLSSDNVFISANP
                     IFK"
     gene            complement(2346..2537)
                     /locus_tag="IVN13_002724"
     CDS             complement(2346..2537)
                     /locus_tag="IVN13_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013156.1"
                     /translation="MYADKEKIEWLLFETGKTAYEISKHSSVAESTIADLIKKKSAIE
                     KMRFDNAAKLTKYAESISS"
     gene            2725..3180
                     /locus_tag="IVN13_002725"
     CDS             2725..3180
                     /locus_tag="IVN13_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013157.1"
                     /translation="MKKLIMVFTSLIAMIVILGGCGSEKNKQDSQDNGVLKPGTVWKE
                     EVGGLVHNLKIIDDTTWEYSESVWHPEQVQITVEKLKDYKGLERYKVIDSGGVDLFIG
                     KTALIVPYNKDGLETFKFIPTNEKEHRDKSEILKEYSTKSGYKLQKTSE"
     gene            3177..3419
                     /locus_tag="IVN13_002726"
     CDS             3177..3419
                     /locus_tag="IVN13_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013158.1"
                     /translation="MIASLFIYPEGGIAVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            3437..3739
                     /locus_tag="IVN13_002727"
     CDS             3437..3739
                     /locus_tag="IVN13_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013159.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQGAATSLKNNLMIFAAVAA
                     VASIVLGGIFFFFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGMFGQTPQGL"
     gene            3808..>5217
                     /locus_tag="IVN13_002728"
     CDS             3808..>5217
                     /locus_tag="IVN13_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013160.1"
                     /translation="MYFTISGNTKKDKELIKKATKKVGKRTAFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVSQVSEVEVNDEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDLVEA
                     NEEPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWERTKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCIE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMERHGQIQQELQAQIP"
BASE COUNT         1736 a          823 c         1066 g         1592 t
ORIGIN      
        1 gttattgttc ctaaaacagg ttctacacat ttagtaacga ttttagcggt cagcttacta
       61 ttacttgtgt tagcaacttt tggctacgca gttctgaagt tcaattaaac aaaaaaacaa
      121 ggctgaaaat cagccttgtt tttctatttg aaaatgggat tggcactaat aaatacgttg
      181 tctgaagaaa gtgatccgtc tgcagaaact tcataagcat gggataaacc aacgatactt
      241 tgattgttgt aaagcaagtt tgcgttatga gcagcgctat ctttccacat aaccatagca
      301 tttactgctc ctgtatctga aaggatctcg tcaacgtaaa ggtagaaact attgatattt
      361 tctcctttag caccgtagca gcctaagctt tctaagagat cttggaaagt accgccgcca
      421 ggtcggtaat gactgaagct tacttgtaat tcttttgcac gaatatctgc agcttgttga
      481 acgatatcat tgaattgcat tggggcaacg ccattttgtt gacggtagct atttaaccaa
      541 gcaagttctt tttgacgcac atagttgttt aaatcactta gtgagatatt tttgtatttg
      601 aaagtgatcg ttcgattttg gtcaacatta ttgattacac gttgtccatt tttatcaacg
      661 gtaacagatg aatcgtcatt tctacacaag aagtagtttt gatcggtcat atttttccaa
      721 gaagtagaga aactatctcc ctcgtaaacc gtttgtgttt cagtagataa aacagcgttt
      781 gtgtcaacgt ttatatggtt cacagtgacg ttgtattgat gagtataggc aaaattgatt
      841 ccttgattac ttgtaacgtt gtttaaagtc acagtgttgc tagagctttc tctaggatca
      901 agaacaaaat tagtgaacat aggtgcttca attgtgatgc tttgtccata aacgacggga
      961 taactcttac tgtaattata gagttcttgg atatttggat cagtatccga gactttattt
     1021 ggacgagctt tcacttcaac tagaaagact tcatcatagt aaaaagtaaa tgtggtattt
     1081 gtgttaatcg tttgtgtttg tgtttcattc ccatttactt gatactgtgg tgcttcaaag
     1141 ttagtcaagt cggttctagc ttttgcagtg taacttgtac cagcttttac tgagacatgt
     1201 tcttctttta gcagcgttcc agtttgtcga ttcatatgac ggacagttac cgcatattct
     1261 gaaggtttag gtttgtgttg taacccttta aatgcagaat caagactatt tttagcatta
     1321 tttacttggg tttgagaagc tttagaatcg tctagtactt ttttagcatt ggataaagca
     1381 gtctgaaaat tattccaaga tccttctgta aaatctcctt tagcagttga ttttacttgg
     1441 ttgtacaagg cttgtaattc cgttttgttc acagtaggag ctggtttttt ctttaatccc
     1501 ttgtaagcag aatctaatgt gtttttggca ctatccactt gtttttgagt cgcttttgag
     1561 tcatttaaca cttttttagc attgtttaaa gcggtttgga atgcgttcca ggtattgtcg
     1621 gtatagtcac cttttttagt tcccttaacg ctcgtataaa gagcttctaa agctgattta
     1681 ttcactttgg gtttagaggt gtcagcacca taccaaccaa taccttcgta tttccagcca
     1741 tagtctttgg ttaaccattg tacttctttc caacttgcag tgtagtgatg cgaaccagag
     1801 gtaacacctg gaaggaaaag gcggtagatc ggtttattat ttttttcggc agaacgccag
     1861 gcaggccctt cataagtcca accgtaattt ttggtcaacc atttcacttc atcccaacta
     1921 gctgtgtaat ggtgatcacg aaggccttta ttgtataaac gatagacaag gtggccaccg
     1981 ctagtcggtg cttcccaagc ggggccttca taattacccc acttaatttt cactaggtga
     2041 tcacgttctt ttgtgctaga ggtgtaaaaa tgttcatggt tatttggatt ataaaggcga
     2101 tacatgacgt tagttgaagc agcgtcagca gatccagcaa gagcaactgt ggcagcaaat
     2161 gttccaatag ctataacagt ttttgggttt aataatttca tgttttcaac tccctattaa
     2221 atgattaaat attttgttta cattcataat ataccacggt ataccgaggt agtcaacggt
     2281 ataccgtgga aattttgaaa taaaaaagcc gccctatgaa gagtgtaagg atggctgttt
     2341 atatgctatg aagaaatgga ttcggcgtat ttggttagtt tggcagcatt atcaaatcgc
     2401 attttttcaa tcgcactttt tttctttatt aaatccgcaa ttgttgattc tgcgactgaa
     2461 gaatgtttgc taatttcata tgctgtcttg ccagtttcaa atagtaacca ttcgattttc
     2521 tctttatcag cgtacattgc aatgctccct cttgattttt tctttattat agctcttttt
     2581 tgtaataaat cacaatatca agaggaaaag tttctataga aaggtgggaa attatgagta
     2641 tccgttcccc gtgtatcaaa atgaggtttg ttgctacttt cgttatttaa tatactaaaa
     2701 taaaacaacg aaagaaggaa tagaatgaaa aagttaataa tggtatttac ttcgttgatt
     2761 gccatgattg ttattttagg tggatgtggt agtgaaaaaa ataagcaaga tagtcaagat
     2821 aatggcgtgt taaaaccagg aacagtgtgg aaagaagaag taggcggact tgttcataat
     2881 ttaaaaatta tagatgatac aacatgggaa tattccgaaa gtgtttggca tcctgaacaa
     2941 gtacaaataa cagttgagaa gctaaaagac tataaaggat tagaaagata taaggtaatc
     3001 gattcaggtg gagtagattt attcataggt aaaacagcct taatagttcc ctataacaaa
     3061 gacggtttag aaacgtttaa atttattcca acaaacgaaa aagaacatcg agataaaagt
     3121 gaaatattaa aagaatacag tactaaatct ggatacaagt tacaaaagac tagcgagtga
     3181 ttgctagtct ttttatttat cctgaaggag gaatcgcagt ggcaaaagct aaaacgccat
     3241 ttgaaaaagc cgcgtctaaa atcttagaac gtcgcggtgt atctgaaaca cagtggaaac
     3301 aagaagtcct tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag
     3361 gatatatcta tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa
     3421 aggagcgaac aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt
     3481 agtagctgcg gttgatattt ttagagcatt acaaggtgca gcaactagtt taaaaaacaa
     3541 tctgatgatt tttgcggctg tagctgccgt tgcctccatt gttcttgggg ggattttctt
     3601 cttctttggc cgaagaggag ctgaaaacgg gaagatgcac gtaggttatg tattgatggg
     3661 ggtaggagtt atattagctg ccgcctctat tgtggtttcg ttatttggaa tgtttggaca
     3721 aaccccacag ggactataaa aaataaatag aaggagtaaa aagtacgtgg ctacttttta
     3781 ctcctttttt atgaaaggag aatactcatg tactttacga tttcaggcaa taccaaaaaa
     3841 gataaagagt taataaaaaa agcaacgaag aaagtgggaa agcgtactgc ctttttaccg
     3901 aaaacaatta aatattctgt cattgaaacg gaagaacagc tacatgtgtt aggacaagtg
     3961 ttagaacaag agaatcaatc ttctgttaaa ttgactctca cgttacatga taaagaagga
     4021 aaaatagaaa aggctggcac agaatacgtt tttggcccac tggattatca aatttcagaa
     4081 ggtttttatc ctacattgat ggatttgatc cgccttgatg tgtttaagga aacggatgaa
     4141 accatgagtg tggaagagtc ttatcgaaac gaaaaactgc tacaagtttt agaagaagag
     4201 ttagagagcg accaaagaaa agtgagtcaa gtgagtgaag tcgaagtaaa tgatgaagta
     4261 ccatgggaac gaccaatctt tccaaagaag gagtcaaaag aaaaagagac agtctatgtt
     4321 gacgaacaag ctgatattcc gactcagaaa tcgatatttg aggaagagaa cgatgtaatg
     4381 ggtcaattgg aggtagatcc ctttgaagaa acaatggagg atgaagagac ggccgaagta
     4441 gaagttgatc tagttgaagc taatgaagag ccaacatgga ctgaagggga ttctcaggaa
     4501 tatattgaac cacttatcag tgaagaagta gagaaaaacg aaacagagat acaaaaggca
     4561 actgtatttg attatatttt ggatcaatat ccgccagagc atgaatggat gaaagaaaaa
     4621 aataaattat ttttaaatga gctttattcg aaatatcaat taccccaaac ctatcaagca
     4681 tttttagaga gtaaagaaga attaatctca cagggtaaag gcacactttt agaacagtta
     4741 gagtctgttc aatcaacaaa ttggcatgag cttgcacaag aagatttatt agccactttt
     4801 gaagaacgtc aacaagaaac agatcaagaa ataaatgagt atgcgacgga acagtcatct
     4861 aattgggaga gaacaaaaga ggaactaaat aaagaagaac aacgagtgat tgaagaagaa
     4921 gtacaaaaaa ttaaagagcg atacgaaaag aaacgcgaca atgcgtattc cttgtgtata
     4981 gagaaaatta ataactttac taaaactaat caagaagctt tatcgaaaga aaaagaaaac
     5041 ttgttaaaag aacgtataaa agaacgaaaa gaagagttct accgaggttt acgtacagat
     5101 aagctaaaga ttagcaaaga attaaatgaa caactgaata aactttatca aacaacgact
     5161 gaagttctta tggaaagaca cgggcagatc cagcaagaat tacaagcaca aattcca
//
LOCUS       DACCKU010000136         5132 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.141, whole genome shotgun sequence.
ACCESSION   DACCKU010000136 DACCKU010000000
VERSION     DACCKU010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.141"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            362..730
                     /locus_tag="IVN13_002729"
     CDS             362..730
                     /locus_tag="IVN13_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP7013161.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            910..1851
                     /locus_tag="IVN13_002730"
     CDS             910..1851
                     /locus_tag="IVN13_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP7013162.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1920..2651
                     /locus_tag="IVN13_002731"
     CDS             1920..2651
                     /locus_tag="IVN13_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP7013163.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2795..4975
                     /locus_tag="IVN13_002732"
     CDS             2795..4975
                     /locus_tag="IVN13_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP7013164.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4995..>5132
                     /locus_tag="IVN13_002733"
     CDS             4995..>5132
                     /locus_tag="IVN13_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7013165.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HL"
BASE COUNT         1711 a          904 c         1137 g         1380 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt caaaaaatag
       61 cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga gaacaccgtt
      121 taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt ccatataaat
      181 acacattatt tattccgaaa agtgaaagcg taaataacaa aaacaaccaa cttatttatt
      241 attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta gattttccca
      301 ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta ggaggaaaca
      361 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
      421 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      481 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      541 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      601 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      661 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      721 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      781 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      841 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      901 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      961 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
     1021 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
     1081 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
     1141 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
     1201 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
     1261 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
     1321 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1381 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1441 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1501 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1561 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1621 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1681 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1741 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1801 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1861 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1921 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1981 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     2041 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     2101 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     2161 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     2221 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     2281 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     2341 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2401 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2461 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2521 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2581 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2641 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2701 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2761 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2821 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2881 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2941 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     3001 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     3061 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     3121 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     3181 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     3241 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     3301 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3361 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3421 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3481 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3541 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3601 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3661 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3721 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3781 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3841 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3901 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3961 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     4021 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     4081 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     4141 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     4201 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     4261 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     4321 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4381 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4441 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4501 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4561 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4621 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4681 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4741 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4801 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4861 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4921 cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4981 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     5041 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     5101 gcgggcgcta tcatttaaac gatcatcatt ta
//
LOCUS       DACCKU010000137         5117 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.142, whole genome shotgun sequence.
ACCESSION   DACCKU010000137 DACCKU010000000
VERSION     DACCKU010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.142"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            58..2781
                     /locus_tag="IVN13_002734"
     CDS             58..2781
                     /locus_tag="IVN13_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7013166.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            2794..3426
                     /locus_tag="IVN13_002735"
     CDS             2794..3426
                     /locus_tag="IVN13_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7013167.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            4325..>5117
                     /locus_tag="IVN13_002736"
     CDS             4325..>5117
                     /locus_tag="IVN13_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP7013168.1"
                     /translation="MKNLQLKFIVDPSIVRWFRIINQFERNQLATKGELAKSNHVSER
                     TIQTDVNKMKDYFSESVQFVSTKSGYSFQKERPVLFLKQKEQLLENEILFELLGNVFY
                     GELEDMTELIDRFHLSEATFRRYLKQIQPVLEEYGLELSLYPLDLKGEEANIRKFFKD
                     FYYEGEMTPHTLVPPRDLHELVQATFYDKFEYFSIGTGTSPAEFYYSLYIAIERVRQG
                     KTIAIPQGLIERVIASENFRLMYSLKEAIQTNYQVALSEEEFCWLY"
BASE COUNT         1745 a          780 c          991 g         1601 t
ORIGIN      
        1 aaataactag tacatatggt aaaataacta gtacatattg tgaaagcagg tggcaagatg
       61 cagcgattaa atgactatct ttatcaacta acattgcttt tgaaatctac gccttcagat
      121 aattattcag ctcgcagtat ttcgcaagct ttacagttga atagaagtac aatcagtagc
      181 tacttaaatg aaggtgttcg tgaagggctt ttcataaaag taaaaagtta cccggtttta
      241 tttttacata gaacagctct tgaagaacta catatcacac tgaataactc cgaaatagaa
      301 tcaattgaaa gcttgttaac aatttctcaa aaacccgcat tagatcaagt aattggctct
      361 aaaggaagtt tgaaagaagc aattgatcaa attaaaacgg cagtcttata tcctggaaaa
      421 gggttgcctt tattattgat cggcgctagt ggatcaggga aaacctttct agcgaataaa
      481 atatatgaat atgcagtaga agaaaaggtc attcacacat cggcaccttt catagattat
      541 aattgtgcac aatatgtgag caatccagag ttactatcct ctgctttatt tggctacacg
      601 aaaggagcat ttaccggagc aagccaggag catactggtt tgctagaaaa agccgatgga
      661 ggagtattgt ttttagatga agtgcatcgc ttatctgaag aagggcaaga gaaattattt
      721 acatttatgg atacgggcga attctctcct atgggggata atagtattcg gaaaaaagcc
      781 gatgttcgat tagtctttgc aacaacagaa aatatatata cgactttctt accaactttt
      841 cttaggagat tgccagttat tgttaatcta cctagatttc aacaacgtcc gtcgtttgaa
      901 cgtctgtcat tgattgatga attttttgtt tctgagagta aaattttagc taaggaacta
      961 tctgtttctg atgcattgat tcatttctta atgaatagtg attttgaagg taacgtgggg
     1021 aatattaaaa atattatcaa gtatgcttgc ggaaacgcat atatccacca gaagagtcag
     1081 caaacaattc aagttaaatt attggatatg cctttagagt acagttataa attcaaggag
     1141 caactaaata aacccagaaa gaaaagagca gatcgaatct actatccaga agaaacacga
     1201 caagtgcaac tagaaagaaa caatcagctt tttgttcaat tttttgacca attacttact
     1261 ggattctctg aagtgattga aaataacaag gatattaaac tatttttaga agaaatggtt
     1321 accaaggtaa cacaagtaat ggatacgctt atttttcaag aggattacga aaaagagcag
     1381 tctttatacg ccattctaaa ctatcatatt cgtcaaagta ttgattttat gcgagaaagc
     1441 tacgggtttg aacaagatgg taaccgggta gtcgctttgg ccaactatct atatgtcaag
     1501 gataatcaag tgatttttga aaatagtctt caagaaaaga aaaccaaaga tcagcttaac
     1561 gaattttttg ctaatgttct tgaaacccct tattggtatg caaaaaaaat cttaagcctt
     1621 ttatctgagt gtttagatca acctgtgtat gaagaggaca ttttctttat tacgctatat
     1681 ttttatagtt tgaatgtcac gaatgttaaa acaggtatta agagtattgt gctagctcat
     1741 ggttattcta cagctagtag tatggcgaat gttgccaacc gtatgctgaa taagaacgtt
     1801 tttcaagctt atgatatgcc tttagatatt acattagaga aaattgaagc aaaattgatt
     1861 caattccttg gagattatcg aacagattca ggcttgattt tattggtaga tatgggttca
     1921 ctaaaccaac taggcggttt attagtgaat catctaaatg gaccactgtt aatcattgat
     1981 catatcaata caccgttatt attggaagtc ggccaacaag tattaaacaa taaaagtatc
     2041 accgaaatag gagcagaaat tgaaacaagt cttactttca aaaagcagtt actacttccc
     2101 aagaaaaaga aaaagaaagc catcattact tgttgttata cagggattgg tagtgcgacc
     2161 cagattcagg agattttaac gaaatgcttg aaagatgcag ttagagaatt agtaattatt
     2221 ccgtatgatt acaaaaagct gttagaaaac aagaattttg agactccttt tcaattatac
     2281 gatgtcatca tgattgtagg tacagaagat cccaaaatta atcagattcc atatattgga
     2341 ttagatcgcc tgattaatgg ggaagcaatt ggcgaatttg ttcattactt ggaaaaagag
     2401 ctgactattg aagtgaacga tttacaaaaa gaattaattt tcaatttttc aattagtaaa
     2461 attgtcgaaa atttaaccat tttggatcca gataaggtgc tatcttttgt acaaaaagca
     2521 attaaagaat tatcgaagtt attaaatttt gatttttcta ataatcagct gtttttgttg
     2581 taccttcact gtagttgtat gattgaacga attgtacgta aagaaagtgt ggatgagcaa
     2641 gaggacattg aagaatatat gcaagcaaat ggagaaataa tggataaaat tcatagcgct
     2701 ttttttgaga ttgaaaggga atacacaatt gaaataccag atttagagtt gcgattattg
     2761 agcgatattg ttcaaggata ggagtgaatt cgaatgacaa tgggagtaat ttttgatatg
     2821 gatggggtac tcatagacag cgagacattt tattttgaga gaagaatgaa cttctttcaa
     2881 gaactggaga tagatccagg atcaaaccag attgatgatt atgtggggaa aacagaagct
     2941 gcaatatggg aaacattagt tcctgaggac aaatcattaa gagaggaact ttatccgaaa
     3001 tatgtagatt atcgaaaaac gcatccaatt gatttcgcta aagatttacg aaaagaagtg
     3061 aaaccgttac ttgacttcct caagaagcgg gatataccat tggcactggc atcttcttct
     3121 ccagttgttg agatagagag tatgctcatt caatgtgaac ttactgaata tttttccttt
     3181 gttataagtg gtgagtcatt aaaacaaagc aaaccacatc ccgaaatata tttaaaagcg
     3241 aaacagcaat tagactgtga cttctttatt gcagtagagg attcaacgat tggtattaaa
     3301 tcagcaaagg cagcaaatat ctatacggtg gcactaaaac aaccaatgca aatcgatcag
     3361 agtgaagcag acatacaaat tgaaagtcta caagaactgg aacaaattgt tttagaaaaa
     3421 ggttagtgaa ttaatggaaa taaggctata tatctgtgct agctattttg actaaccaaa
     3481 aaaacttgct ataattcagg tgtagtaaaa ggtgctggta acacctttta cgaaaatgct
     3541 tcggcagtga taatgggatt ggctttttaa gttttttatt ttgctcgcaa tccctaaggt
     3601 catgtgaatg atcctaggga ttgctagaat attttgcaac attctatcta tttcagaacg
     3661 taaccttccc cagttcattg ggattcatta gcatacaacc aacatttcaa aaagaaattg
     3721 cactacacca ttattagcta ttttgactaa ccccaaaaaa cttgctataa ttcaggtgta
     3781 ggtaaaaggt gctggtatca ccttttacga aaatgcttcg gcagtggtaa tgagtgtggc
     3841 ttaaaggact agtcctttaa gctttttact ttgttcgcaa tctctaagat caagttaatg
     3901 atcgataaga ttgctagaat attttgcaac attctatttc agaacgtaac ctttcccatt
     3961 ccactgtgat tcattggcaa ccctcctcac aaccaacgtt tcaaaagaaa ttgtaaagtt
     4021 gttggggtac cacgaccgaa caatactaca cctgaatgat agtaagttgt ctcattaagt
     4081 atagcatatc ctctttttgg gtaaatagaa atcggttaca ttttttatcc aagaccaagt
     4141 tttttcattt ggtcttattt gctataccca aacaattccg cagtatagtc gacggtattg
     4201 attttatcag gtgagtaata gaaacttgtt ttccttcccc aaaacattac ctataaagga
     4261 gaaaaaatgg tatcgtaatt atataggtat aggatgaaat ttttttctaa gaggagggtt
     4321 gttgatgaaa aatttacagt taaaatttat tgttgatcca tcgattgtac gctggtttcg
     4381 gattatcaat caatttgagc gcaatcaact tgccacaaaa ggagaattag caaagagtaa
     4441 tcatgtatct gaacgaacca ttcaaacaga tgtcaataaa atgaaggact acttttctga
     4501 aagtgttcaa tttgtttcaa cgaaaagtgg gtattctttt cagaaggaaa gaccggttct
     4561 ttttctcaag cagaaggagc aactactgga gaacgaaatt ttatttgagt tgttagggaa
     4621 tgtcttttat ggcgaattgg aagatatgac ggagctaatt gatcgttttc atttatctga
     4681 agcgactttt cgtcggtatt tgaagcagat tcaaccagtt ttggaagaat acggcttaga
     4741 attgagtctt tatcctcttg atttaaaagg agaagaggca aatattcgca agttttttaa
     4801 agacttctat tatgaggggg agatgacgcc ccatactctt gttccaccaa gggatcttca
     4861 tgaacttgtg caagctacat tctatgataa atttgagtat ttctcgattg gaacagggac
     4921 ttctcctgct gaattttatt atagtttgta tattgcgatt gagcgtgtga gacaaggaaa
     4981 aacgatagcc attcctcaag gtctgattga gcgagtaatt gctagtgaga attttcgctt
     5041 aatgtattcc ttgaaagaag cgattcaaac aaattatcaa gttgctttat cagaagagga
     5101 gttttgttgg ttgtatt
//
LOCUS       DACCKU010000138         5087 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.143, whole genome shotgun sequence.
ACCESSION   DACCKU010000138 DACCKU010000000
VERSION     DACCKU010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.143"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(47..871)
                     /gene="iolB"
                     /locus_tag="IVN13_002737"
     CDS             complement(47..871)
                     /gene="iolB"
                     /locus_tag="IVN13_002737"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAP7013169.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IVN13_002738"
     CDS             complement(910..2826)
                     /gene="iolD"
                     /locus_tag="IVN13_002738"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAP7013170.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IVN13_002739"
     CDS             complement(2837..3871)
                     /gene="iolC"
                     /locus_tag="IVN13_002739"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAP7013171.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(4028..5044)
                     /locus_tag="IVN13_002740"
     CDS             complement(4028..5044)
                     /locus_tag="IVN13_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP7013172.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
BASE COUNT         1337 a         1270 c          909 g         1571 t
ORIGIN      
        1 acctaactaa acctaactaa actttatgta tctaacagct taataactac ttgcgctgca
       61 agatccattc gtgtttcttt tcattgtgga atttccaaat tcgctcaggt cccgccatca
      121 catttaaata gtaggaagta tacccgtcag gtaccccaac tggatgatag cccttaggaa
      181 cttcgacgac attttgatgc tctaccgtca tcgtttcatc tagtgaacga tcttccgtgt
      241 agacccgctg gaaaacaaat ccctgctctg gatcaagctc atggtaataa gtttcctcta
      301 aaaaagactc atgtggcaga ttctcttgat cgtgcttatg aggaggatag ctggaaaaat
      361 taccgccctc ggtataaact tcgacgacca gcaggctgct ggcagtatcg acattatccg
      421 gtaaaatgtt gtgaacaagt cgtttattct gatagagtcc acgttcttcg acggtattat
      481 cctccgcgcg aatcacactt atcggcaaag cttttttcgc tggtgaatag caaagcgcca
      541 atcggacatt gtctgtagtt gccacaagtt caaacgaatg ctccgccgga agataaatac
      601 tgtcggttgg aattttttca aaaacagatt ctcgtgttcc taaatcttca taccaagtgt
      661 ctgctgcttt aaccttaatt tttccagtca aaatgacgat acagacctcc tgttgactgc
      721 cggtttcctt gtaagaagct cccttttcca gctccaaaat ctgaaattcg atataactca
      781 aagcagaatt agactgccga atattttgga tttgttttac tccaggtgcc agttctttgt
      841 ttaatggttg atgcttcaat gtcttttcca atcaatccac tccttatttt caatcacaaa
      901 aagcaatatt taatactttc tgatagtggc aagtttcgct tgtttggctt tgtaagcctc
      961 ctgcatggct gctttacccg atacctctga gataccgacg ttccaccagc tgccgtcgta
     1021 gccttctgtc atggttttag gaagtacctt tatctcaatc aacgtcgatc gcgtttgctt
     1081 cttcgcatcc tcgaccgctg cttgtaattc tgcaatcgag cttacacgat acgtttttgc
     1141 accatagcct tcagcaactt tggcgtaatc gatgttcatg atctcgtcct tggtatttct
     1201 gaattcggtt ccttggctag ctccgccatt atccatctgt aaattattga tacagccgaa
     1261 accagaatta tcgaaaagta caatgttgat ttttttatca tattgcaaac tagaaaccag
     1321 ctcgctatgc aacatcaaga aactgccatc gcctactaag gcatagacct cttgttctgg
     1381 tcgagccatt cttacgccca tcgcaccagc gatttcatag cccatacaag aataaccgta
     1441 ttccaaatga taggtattct ccttcacagg gttccataag cgctgcatat cccccggcaa
     1501 tgaaccggct gaaccgatca cgatcgcatc ctcatcaaca aaatcattta aatgaatgaa
     1561 gacctctgtt tgcgtcaaat tcgtagccaa cgcatcagca tattcattca agatttcttg
     1621 agaaaaatga tctttgattt ctggcgtaaa atctttacgc ttaaaatgcg cggcttttaa
     1681 ccgatttcgc tcactgatcc attcttcctt cagttcatcg atcttctcgc cataggcggt
     1741 ttggtaacct tcgatcattg gggcaagctg ctccaaggtc gctttcgcat caccaaccac
     1801 ttgaaaggct tccagcttag cggcctgttg tctgcttacg ttaatattca tcattttcgc
     1861 ttgttcaaaa ttaaaaatac ttttagaagc tgaggtaaaa tcagtgtagc gtgtgccgat
     1921 ccctatgatc agatccgctt gctgcacagc tttattagca gccgaagttc ccaaaatccc
     1981 cattccgccg agattgtttt taaaatccca cgaaacagtc gatttacctg cgtgcgtttc
     2041 aactagcgga atctgacaat ctcttgagat ttgctcaaga atctcaccag cctccgaata
     2101 tttgcagccg cctcctacga tgatgacagg tcgtttactg gcctttattc gttgactcgc
     2161 ttcagcaagc tcacgagcgg tcggaatcct gcgatcaata taatgtactc tttttttgaa
     2221 aaattcctcc ggataggcat aggcttctgc ctccacgtcc tgcggcaaac aaatcgttgc
     2281 cggacccatc gttgccgggt tcgtcatgac ttcaaaggcc cgcaataacg cggacatcaa
     2341 ctgctctggt cgattgatgc ggtcccagta gcgagaaacc gcttgaaatg catcatttgt
     2401 cgaaaaggct acactgcttg catgttccag ctgctgcaag accggatcag gctgacgtgt
     2461 agcaaacgta tccgccggta aaaaaagtac ggggatatta tttgcgaatg ccgtacccgc
     2521 tgctgttaac agattggccg cgcccggtcc aacagatgtt gatacggcaa aaattttctt
     2581 gcgtaaattc tgtctggcaa aggcagtagc cgcatgggcc attccctgct cattttttcc
     2641 gctataactt ttcaaatgtc cgggctctaa ttccagcgcc tgaccgatcc ccaaaacatt
     2701 tccgtgacca tagacatgaa agataccttc aacataaggc gtttctttgc cgtcgatgct
     2761 gatgtattgt tgattcaaaa attttagtaa tgcttgtgct gttgttaaac aaaccgtctt
     2821 ttccattttt ttcaccttaa atccgagcga tcatttcgcc gtatatttct ttctccaatg
     2881 taataaaatc ttttacctcc tgagtcgtcg gtaaagaatc tgaacagtta ttgcttttga
     2941 ccatcattga tgcttccgct gagccaaact ctaaacaatc gatgatctcc cagccttcga
     3001 ataaaccgta taagaaaccc gagccgtaac catctccgcc gccaaagcct ttgcgtgctt
     3061 ccaccggaaa cggcttgatt gaaaactgct gctgatcggc cgtataagca gtcgatcctt
     3121 tcattccatg tttgattacg acaatctcag caccacaact gtgccaaaaa gcagcgcttt
     3181 cattatctga catgttaggc tggatcaact tctcagttag atcgaactct tctcttgaac
     3241 ccatgataat gtctgattcc ttagcaatca tcgaatagta aattgaaata tcatcgttgt
     3301 tcttccagtt ataagcacgg taatcaatat caaaaataat tttgacatta ttccttttcg
     3361 ccagcatcac tgccttgatt acggcttctc tggacgggct ctctgctagt gcagttcctg
     3421 agattaatag tgcttttgac cgtttcaagt agtcctcatc aatatcctca acgctaagct
     3481 gcaggtctgc ggcttgattt cgatacatca aaatattact ttcctttgat gagagcattt
     3541 cggtaaaagt cagccccagc ttttcaccat gctgcgcttt gctgatctga gacgtatcga
     3601 tgccttgttc attgaaataa tcgatcacat attcgccaaa ctgatcgtcc gagactgttc
     3661 cgataaaacc agctttcatg ccatgtttag taacacccac agcaatattg gcaggagatc
     3721 cgcccacaaa tttttcaaac atatggacct ttttcaatgg tttaaactct tctttgactt
     3781 gatcattgta ggcaggatta aaatcaatag cgactcttcc taaaagcagc aagtcaaatt
     3841 cttttgattg gtcaaacttt atataactca atatatccac ctctttatcc gttcttctat
     3901 ttctgaggca agtatagcac gccaaaacaa agcgtgcaat atttatttta gaacgcaata
     3961 ttgcgtgctg tttgaaaatg acggcaaaaa aaacgtgaat cgccttacat cacgattcac
     4021 gcctagatta aatattgtat tttgtcttaa taattatatc agtaaatagc gcatcttttg
     4081 taaccgtttt attattttgc agctgattcg ctaacatcag taatgaacga tagccttgaa
     4141 caaatccatc ttgatcgatt agaaaatcaa cctgatcgtt tttcagcatc tcgatattgt
     4201 tcttagtcaa atcataaatg attacaaagg gccgcttgct ttcatcgatt ttttccaacg
     4261 ctcgggcaat tcccgcttgt ccgccagaaa agacgactac gccatctaag tttgaataat
     4321 tctccaaggt gttgcttaaa atcttttcaa cctcagcact cgaatcaaag ctgctctgaa
     4381 cacccgtcag ctccagttca ggaaacgact ctttcaattc ttcaacaaag cctgccaccc
     4441 gtaagctgtt gacgctattt ccaaagtagc cggtaatcgc tagaaccttg ccctttcctt
     4501 ttgtcagcat acctaacaat cccgcagccg tttttccgct tttgaagttg tccatcccaa
     4561 caaaacactg tcgattcgta ccaagaatat ccgaattaaa ggtaatgatg aggatgcctt
     4621 gatcggtcaa ttgattaatt ttttctctga taccatcact atctactggc atgatcgcga
     4681 tcccttttac tccaagctcc accaatcgat ccaacgcctg cagctgggcc gcttcatcga
     4741 ccccgtctac ctcttctaaa aaacaatcaa tgtttcgctt ttttaaatcc ccgataatag
     4801 attccagacc tgatcggatc ggaatcataa aagaagattt cgccaattga gtgacaatcc
     4861 ccagcttgag cgttgtctct tttatgacct tctttttctt tggaacatag ttcatttcct
     4921 tggcgattgc tctgattctt tctgcaacct caggattgat ccttcctcga tcgttcaatg
     4981 cccgatccac tgtccccctt gacactcctg ctttctcagc tatttcttta attgtacccg
     5041 ccatcttgcc tctccttttt tcctattgtg tttattttat cacaaaa
//
LOCUS       DACCKU010000139         4877 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.144, whole genome shotgun sequence.
ACCESSION   DACCKU010000139 DACCKU010000000
VERSION     DACCKU010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.144"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(21..983)
                     /locus_tag="IVN13_002741"
     CDS             complement(21..983)
                     /locus_tag="IVN13_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7013173.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(985..2424)
                     /locus_tag="IVN13_002742"
     CDS             complement(985..2424)
                     /locus_tag="IVN13_002742"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP7013174.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2609..4555
                     /locus_tag="IVN13_002743"
     CDS             2609..4555
                     /locus_tag="IVN13_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP7013175.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1575 a          812 c          800 g         1690 t
ORIGIN      
        1 attaaaaaat taatttaacg ttaaatactt cctccagaaa gtattgagat tggtaaaata
       61 taatccttat ttgtcttttc gcctttaatt tttttcagaa gaatatcaac aataagactt
      121 gcaatttcat caattggttg tcttattgtt gtcaattgtg ggaaaaattt ctcaataaaa
      181 gaggtaccat cgtatccaat aatttttaaa tcttctggga tattaagttt aagttgatga
      241 gcaattttca tagtgagaat ggctgttaaa tcatcagaaa caaaaatacc atctggtttg
      301 tgatttataa tagttgactt tatttccatt tcacgacgga tagtagacag attattagga
      361 attttgaaaa tttttccatt tggattttga aaagaaaaac ctagtgcacg taattctgtg
      421 ggagaatccg tgttatcatt tcctgtaatc ataatggtat tttgacaacc atttttttgt
      481 aaagtcttag ctgcaagttt tcccccttca aagttatctg aagaaacaat ggaaacatta
      541 ggagctaaat ttctatcaaa agcaacaata ggtgctctca cacgctcata atcagcaatt
      601 ccaagattat ggcttgatga gattatgcca tcaacttggt tggcttcaag catttcaatg
      661 tattctctct ctttagcagg atcattttga ctattgcaaa taatcgtttt atatccatgt
      721 ttaaataact ctatttcgag atactcaatg agttcagagt aaaagatatt actaatatta
      781 ggaaaaataa gaccaataag ctgaggtgac tttccttgaa gtccacgtgc taaattattt
      841 ggtttataac caagagtttt catagcttca tgaacttttt gttttgtttt ttctgataaa
      901 tatcccttat tattaatgac gcgtgaaacg gttgttgggc taactccagc taattcagcg
      961 acatcagtta attttgctac cattttaata ttttaattca aagtattttc cagtaggttc
     1021 gcctgatttg atttgaatac cattttggtg attttcaggg aatacacgac tagtaaacac
     1081 tttttctcct ttattaataa aaatctcaat gattgagtta tctacaaaaa tgttagcagt
     1141 tgtttcttta gtatcaattg agcattcacg aatcgtacca aagtcagtgg catattgaat
     1201 gcctgctttg ctgcgatcta aaataatttt tccttctttg gtatctacaa tcaagctaag
     1261 tccatttcct ttactatcag agaaaagaag taactccgat ttctgatttg gaggaaaagt
     1321 tagttcaagc tcataagtat tatttgtttc tgcttttgct gtaaattgtt cagaactagc
     1381 acgtaatgat gtaatagctt ctactggata ttggtaaagt ttaccatctt ttattgaaag
     1441 ttcttttaca agactcattg ctccctgata atcatactta tcagttggat aatcgatatc
     1501 aggtagtcca atccaactga caatcagagc acgaccatca ggagtattga atccttgagt
     1561 cgcatacgct tcaaatccat aatcaagatt ttgaatttct gaagcgccca caagttttac
     1621 tttttctatg tcaagcgatt gacagatctt aaaagtattc ggatagatat tcccataatt
     1681 tagctcagat ttatccaacc cttgaggaca ataaatgaga acaggttttt tgtctacaaa
     1741 tactagattt ggacactcaa tcatgtattc tgaaccagtg ccgccaaaat caaggttgcc
     1801 aacttcctcc catgtttcca cgttattgtc atccgctttg taaagtttga taaagcctga
     1861 tttttctaaa ttttgtgctc caacaatcgc atacaattgt ccttcataat taaacatttg
     1921 tggatcacga aaatgatcag ttacatcagc aggtttatga ataagaacat tctcaccttt
     1981 cgtaatgttg tagtctttat ccatccatgc tccgatttga agtgtatcac gttcccaatt
     2041 ttcatttcga acgtttcctg tatagagtaa gaatagattc ccctctattt catatgcaga
     2101 accggaataa gcgccgtggc tatctcgttt tgtgtctgga agaagttctg ctcctgttac
     2161 atgaaaatga actaaatcat ctgattcatt gtgtacccat tctttcaacc catgagcagc
     2221 tccaaatggc caattttgat aaaaaagcgt atattttccg ttaaaaaaag aaaaaccatt
     2281 aggatcgttt aacaatcctg ttttaggttc gatatgatag ctagcacgcc aaggtgatcg
     2341 ttttacatta tccttaattt tatccatctc atctttagtc caatcagaat aagcacgata
     2401 tcgtatttct cgaggtaagt tcatatttta ctccttcaat ttatcttttt acaactagta
     2461 tggtaaacgt tttccgttga aaaatcaatc atttcattta aaaaaataaa ctatatgaca
     2521 aacgtttgac atatttttaa gatatgctaa aataaatttg catttcagtg aaccgttttc
     2581 acgtaaaaat aaaaaaggag tgattcgaat gaattacagt aaagtagctt ccgacgtaat
     2641 acaagcggtc ggaaaagata accttattgc agctgcacac tgcgctacac gtcttcgtct
     2701 cgtactaaaa gatgacacga aagtgaatca aaaagcttta gacgaaaatc cagatgtgaa
     2761 aagcacattt aaaattgatg gacaatatca agtgattatc ggtgcaggtg atgttaattt
     2821 cgtttatgac gaacttatca aaaaaactgg actttcggaa atttcaacag atgatttaaa
     2881 acaaattgct agtaaagata aaaaattcaa ccccattatg gcattaatta aattattatc
     2941 agatattttt gttccaatta tacctgccct tgttgcaggt ggtttattaa tggcattaca
     3001 aaacttttta acagctgctg gactttttgg accaaaatca attgaagaaa tgtatcctgc
     3061 gattgaaggt atttcctcaa tgatccaact gatgtccgct gcaccattca tgttcttacc
     3121 aattttagta ggtatttcag ctgctaaacg ttttggagca aaccaatttc ttggtgctgc
     3181 aattggtatg atcatgacaa caccagatct tggtggctca actgaatatt ggaatatttt
     3241 tggttatcat gtttcacaaa caaactatgc ttatcaagtt attccagttt tagcagctgt
     3301 ttggatatta tctatattag aaaaattctt tcataaaaaa ttgccatcat ctattgactt
     3361 tacattcacg ccattattat cagtaatgat tactggattt ttgactttca cagttattgg
     3421 ccctgtaatg ttattgttgt ctaatggaat tacagacatt attgtttggc tatataacac
     3481 tactggattt attggaatgg gtatttttgg aggaacatat tcacttatag tcatgactgg
     3541 tttacatcaa tcatttccag ctattgaaac tcaattactt tcagcgtgga caaatggtat
     3601 cggacatgga gactttatct ttgtagttgc atctatggca aacgttgctc aaggtgctgc
     3661 aacatttgcc atttggttct taactaaaaa ttctaagaca aaaagtcttg cttcctctgc
     3721 gggtgtctca gcacttcttg gaattacaga gcctgccttg ttcggagtta acttaaaata
     3781 ccgctttcca ttcttttgtg ctttaattgg ttcaggaatt gctacagcag tagcaggatt
     3841 acttaaagta gttgctgttt cacttggttc agcaggtttc ttgggatttc tttcaattaa
     3901 tgcaacatct attccatttt acgtattgtg tgaattgatt agctttgtag ttgcttttgc
     3961 aattacttac ttctacggta aaacaaaagc tgctggtatc tttaaagcag aagaaattgc
     4021 tgaacaaaca ccagttaatt cttcaaaagt agcaacaaca gatccaatat ctactgatgt
     4081 tgaatcaaca caaacaaaag tttcatcaga aacaattgtt agtcccattt ctggtaaagt
     4141 gattagtctt tcttcagtaa atgatccagt cttttcatca gaagctatgg gaaaagggat
     4201 tgccattaaa ccaaatggaa acacggtcca ttctccagta gatggtacta ttcaactcac
     4261 gtttgaaact ggacatgctt atggtattaa atcaaataat ggtgctgaag ttctaattca
     4321 tattggtatt gatactgtat caatgggagg aaaaggattt acacaaaaag ttactactaa
     4381 tcaaaaagtt agaaaaggcg atattcttgg agcatttgac cgttcaatga ttgagaaaga
     4441 aggtcttgat gatacaacaa tgattattgt gaccaatact tcggattatt cagaagtaaa
     4501 tatagtagct gaaaatacag tcactgaggg tactgattta cttgaaatca aataaatata
     4561 tttccctgag taaagatgga attttctttc caaaatcaaa taatatagat taatcaaata
     4621 tttgagaata acattttatt taatcttttc atatatcaat tgtatgaaaa attattagaa
     4681 ttttaaaaaa tgataaaaat tcaaaaagaa aaaatagtag ttatttactt gtcatgttac
     4741 taaataaaaa aataatttaa atatgatata tagtcattta tttagtgatt tcattctaaa
     4801 ataaagggag aaatgtctat gctatacggt gcaattgaag caggcggcac aaaatttgtg
     4861 tgtgccattg gcgatga
//
LOCUS       DACCKU010000140         4860 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.145, whole genome shotgun sequence.
ACCESSION   DACCKU010000140 DACCKU010000000
VERSION     DACCKU010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.145"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..1217)
                     /locus_tag="IVN13_002744"
     CDS             complement(<1..1217)
                     /locus_tag="IVN13_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP7013176.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVE"
     gene            1278..1853
                     /locus_tag="IVN13_002745"
                     /pseudo
     CDS             1278..1853
                     /locus_tag="IVN13_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2034..3227)
                     /locus_tag="IVN13_002746"
     CDS             complement(2034..3227)
                     /locus_tag="IVN13_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7013177.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3311..3514)
                     /locus_tag="IVN13_002747"
     CDS             complement(3311..3514)
                     /locus_tag="IVN13_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP7013178.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3771..4013
                     /locus_tag="IVN13_002748"
     CDS             3771..4013
                     /locus_tag="IVN13_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013179.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3935..4168)
                     /locus_tag="IVN13_002749"
     CDS             complement(3935..4168)
                     /locus_tag="IVN13_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013180.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4175..4606)
                     /locus_tag="IVN13_002750"
     CDS             complement(4175..4606)
                     /locus_tag="IVN13_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7013181.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1324 a         1109 c         1004 g         1423 t
ORIGIN      
        1 atttctacaa aaaaatagtg attctgtttt gaaaattcga tttttagttc tgcattttcg
       61 taaacatcca acattttttt cgcactgtat agacctaacc cgccattttt tttagaggtt
      121 tttcctcttt tgaccatctc cgatagcgtc aaattcgttg gatttgcctt gttcgctata
      181 ctaaaacgtg atccctcttt ttcttccagc agattgatcg taatcggact ctgatcggct
      241 acacgatgat cgaatgcgtt agaaataaaa atagataaac agcgaataaa aacaaccaga
      301 tcaatcggga ttgttttcac ttcgacagga ataactactt ggatcgggat tttctccgat
      361 tctgcttttt ctagaaattt taaaaagaca cccctgacag ctggggatac aatatttgtt
      421 agctgttgta tttgcggttt ttcaaaaata gctgcagaat gatcttctaa tatttctaaa
      481 aattccaacg tttctttttt attttcctga agagtaattt ttaatgaaaa cagtagactt
      541 ttataatcat gtttgaattc ctctagatga tggtaatatt cttcatttat ttgatacagt
      601 tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat aatggaaaaa
      661 aaaaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata attagatgag
      721 cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa aatacaaata
      781 cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct cgtctgtttg
      841 tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa aaaacttgct
      901 gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa aaagctgagt
      961 ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt tttcgtagta
     1021 aaataaaaaa gaaacatcaa taataagcca actaggtaag gaaagctcac tttagggata
     1081 ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt tactagcagt
     1141 tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt caaataaagt
     1201 gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac acatattttt
     1261 ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag tttcacagcg
     1321 aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag ctcacttcta
     1381 tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat gttttcttta
     1441 tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa atacgtaaaa
     1501 aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca gtagagatca
     1561 tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta tctgaacaaa
     1621 ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat gtcgataaaa
     1681 tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt ttatatattg
     1741 aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac aactacagaa gataaattag
     1801 tgaatacttc tttaaaacaa ttgaagacag agttagaaaa gtatgcttac tttttcttaa
     1861 aaattatatg ggattttaga ttatcttttt agaagttctt gaatgtcggt atagcccgtg
     1921 tttccgggct tttccggcat ttttgatatt gttagatact tacaaatgtt cttattacct
     1981 ctcaccattt ccccctcaaa agtagtaaat ggagtagtaa aaccggcctt gtgtcaggct
     2041 gccagccgtt ccatttctgc ccttgcggaa tcgaatgtgg cgtgtgcata atagttcagc
     2101 gtcatggtga tattggaatg tcccataatg tactgcaagg ctttggggtt cattcccgca
     2161 ttggctaacc gggtgcagaa tgtatgacgc atagcatggg gcgtcgtcgt tttcggcaac
     2221 gcttcttcat ggctcttgtt gtacttcttc acaagcctgc caaacatccc gccataatct
     2281 accgccgtca tgggatagcc gttctgtttg agaaacagga aattggcata cccgtcaata
     2341 atgaacggct ttgcaccttt gcggtttttt atcactcgct ggaacgcctg atacacttcc
     2401 tcattcatgg ggatttttct gacgccgctt tgggtcttgg gttcgtcaat atagtaaccg
     2461 acgccggtat ttctcaaaag ctggtggctc acgatgatta cccggttttc aaaatccaaa
     2521 tctttgtccg tcagtccgca cagttccgaa atgcgaagcc ctgtccccag cagaataacg
     2581 attgcgtcat aatgcttttg gtaaacctta tcaccttgaa taaaggacag aaaactttct
     2641 tcctgtgctg gtgtaagagg cacctttggt tccgtatcgt cgtccagaac gtcactaagc
     2701 tggaagtcaa acgggttttt tcggatgcaa tcgtcctgta tggcggtgta aaaagccgct
     2761 ttcagggaac gcttgtcgtt attgatggtc ttataagaaa ggcccttttc tttcatccga
     2821 agcgcccatt cctttgcgtc ggacagcttc acactgtcaa tcgggcagct ccccagccga
     2881 tcttgttcca gaatacgcat cagccgttcc cggcccagtt tggtgttgtg cttcacattg
     2941 ccccggtgcc tgatctgctt ggcgtagagc tggcagacgg tcattttctt gccgatggtg
     3001 tctatcccgt cgtcaaggtc tttctggatt tctttctcct tttcccggag tgaaatatcc
     3061 tcccgtttac ctgccggggt cttatccgta ggcactaact tccatgagta aacaaactgc
     3121 ggttttccaa aggtatcaac atatttgtaa gcatatcgtc cgtcttttct ctggctctct
     3181 ccagaccgca aaacgcgatt tttgctgtct cgtctttttt ctgacatctc gtcatgctcc
     3241 tttccatgac ggaaagagcc gcatatatgc ttataaatta ttataacata tctgcggccc
     3301 tttttcacta ttagattgtg tccaaagtgt caattatttt ctcaaactgc ttgcgtttga
     3361 tctggatgcg gttgccattc ataatgaccc agccagaggc cggattttcc tcggccagct
     3421 tacgcaattt gttttctccg atacggaaat atttagacgc ttcctcaatc gtcagcgtgt
     3481 atttttccca aataggcaca tcatggttca taatgcagcc ccctttcaca aattcaaagg
     3541 tttgtgtgat ttaggggcat agacggacgg ctgctggcac agctccacgg gactttcacc
     3601 cccgcgtggt tctcacgcgg ccctacccat tgcctgcgac gcttttaacg ctcggactgt
     3661 ggctataagg gagtatcata gtcctgcctg tacgtcgtcg cccgcaagcc gccacgctca
     3721 cattgcggcg tggtgttcct cgctccgctt ttcccgtagg gaaaagtatc atggcgcacc
     3781 agccgtccgg ctcggcacag acagaatgtg tccgtatgcc ctatgatgcg ccgccgttat
     3841 cttgcgggca tatttatcaa ggtgcgatca ggccaccggg aaaacaccga aacggggaaa
     3901 gggtaagccg tttcatgctg ccggggccgg ttcgtcagtt cgtgacgata gcgtggatga
     3961 gcttgatttc cagacggcgc ttcatggcct cgtccagaca cacatgcgga tagccgtttt
     4021 cgtcatagag cgtccgggta cacagcttgt tcatgtaacc gctgtaatac tgcaaaacgc
     4081 gctctacggc tccggcatcc ccagcgcggg cggcctcgat caccgaaagg ggcaaaagct
     4141 caatgccctt gtagttcggg ttcttcatcc gcgtctaacc tcccttcggc atcaacgcct
     4201 ttagctgttt ccgcagctcc gataaggtat ttgtcctgtg ccgctgaacg gcactccggg
     4261 acattcccac aaggccgccg atctcaccat ctgccatatc cagaacacag tgcaggatta
     4321 aaatactctg ctccatgctg ggcagcgcgg caaaggcttc ggcaacaagc tcattgttga
     4381 tatgtaggtc atagccatgc gacgaaaacg taaagctgtc gctggggtag tgatctactg
     4441 tacagagctt gtccaaatct gcctgtgaca aagagcttag tgacacttca cggtcacggc
     4501 ggcgcttcat ttcagccaag tagcttttgg cctcgttacg cagtaccgtc ttgcaaaaag
     4561 catcgaacat gcaccgcgct tcatagtcgc gggggatagc atccatcttc tcaccccctt
     4621 tctgtggggg acttaggtgg ttctctccct ttccgctaac aagactttga caaggccgcc
     4681 agctacccgc taaatacctc ctttttgaaa atttctcaaa aaattttttt ggggacagcc
     4741 gggccgcaaa agacgacaaa aatcgcccgg caggtcgcaa acctaccggg cgagaaaatg
     4801 ctttgctatg ctgctgattt acatggtata gtacatactc ataaccggaa tagccctgtg
//
LOCUS       DACCKU010000141         4760 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.146, whole genome shotgun sequence.
ACCESSION   DACCKU010000141 DACCKU010000000
VERSION     DACCKU010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.146"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            41..712
                     /locus_tag="IVN13_002751"
     CDS             41..712
                     /locus_tag="IVN13_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP7013182.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            771..1211
                     /locus_tag="IVN13_002752"
     CDS             771..1211
                     /locus_tag="IVN13_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013183.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1223..1891
                     /locus_tag="IVN13_002753"
     CDS             1223..1891
                     /locus_tag="IVN13_002753"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP7013184.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1955..2578
                     /locus_tag="IVN13_002754"
     CDS             1955..2578
                     /locus_tag="IVN13_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP7013185.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2634..3020
                     /locus_tag="IVN13_002755"
     CDS             2634..3020
                     /locus_tag="IVN13_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP7013186.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3233..4624
                     /locus_tag="IVN13_002756"
     CDS             3233..4624
                     /locus_tag="IVN13_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP7013187.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1587 a          783 c         1012 g         1378 t
ORIGIN      
        1 aatatgctat tttaaaaatg atgaaaaaag ggggagaaaa atgaattttt tagttgtttt
       61 aaaagatact ctgattgagc gatctacatt tctatatgtt caattgcagg aaaataaaac
      121 attgctgcat ttttcaccag agttccatct agaggggcta acattaggag aaatctatca
      181 agcagaaggg ctgtcggctg ttttgcactt ttttaaggta gaattggaat taccagtaaa
      241 agattatatc gagctttctc ttgaatcttg ggatcgagca gtggttgaac tgtttccaga
      301 aggtttaatg atcgatacaa acgatgggaa gatccatctg actgcagcag aactgcaaaa
      361 acaaatgcgt tatcaaatag atgacgaaaa ttctttttct atttttagac gtcagcaaaa
      421 gattctaaaa agctttttaa aagtgatcag taaaaaacgg aatctgatca aaacgacaca
      481 gctgttgaaa aaatatccag gtctgcttca cacgtctatt caatttcctc aattgattac
      541 gatgatccat cgttttatac gtatgcaaca gctaccatct aagaagctgt ttcttccatt
      601 gacggacaca tttcgagtag tcaacagaga agataaaaag aaagtgattg tgattgactt
      661 taccagaaat cggaatgtcc ttcaccaagt tgcaatggaa aaggccaatt aaggcttttt
      721 ctttttggat ttttttgtca taataaatat tagatgatgg aggagataat atgatgcaaa
      781 catggttaga aaggctaata cgatttcagc agaactatca caaacttatg cagggaagat
      841 atgcgaaatt tgatcagttg aatcgcggtc tattgatcgc tgcgcttgtc tttagtttgc
      901 taagtcgttg gttgccctat agaatcggac aattgttgtt catcgtcttt ttcggatgga
      961 tgatatttcg tttcctatca aaaaaaatct accccagact aaatgagaat caacgttatt
     1021 tgaaatatct tgatagagtc aaagaaaaat atcatcagat aaaaaacaaa aagcaagtca
     1081 aaatggatcg aaaaagttat actttttttg agtgcccgaa ttgtcatcaa aagcagcgtg
     1141 cacctaaagg aaaaggacga atacgtgtga catgcaaaac atgtggtata aaattcgaaa
     1201 cgaatgttta agggagaaaa gaatgaaatt agtattatta cgtcatggcg aaagcgaagc
     1261 aaattttgaa aattactgga caggatggct agatgtcgcc ttaacagaaa aaggaaaaga
     1321 acaagcagca gttgccggtc aaaaaatgct tgaagccgga cttcaattcg atgccgttta
     1381 tacctctgtt ttgaaacgtg cgattcttac cggacaagtc gcactagaag aaatgggtca
     1441 gttatggctt cctataataa aaacatggcg tctaaatgaa cggcattatg gggatcttgt
     1501 gggtaaaaac aaagataaga tgaaagaaat atacggaaaa gatcaagtaa aaacatggcg
     1561 cagaggatat acggaagttc cgccgttaac cgcagaaaat agatttgacc gcagatatga
     1621 tcacttggat ccgcatttga tcccatacgg agaaagtctg gaaatgacag tgaatcggat
     1681 cattccctta tggcaagatc atctagcacc aaaactaaaa gatcggcaag atttactagt
     1741 tgtgggtcat ggtaatagca tccgagcact gacaaaatat ttagaagatg ttcctgaaga
     1801 tcaaatggat acgatcgata tcccaaatgc acaaccaatc cagtatacat tagcagatga
     1861 tttgacaatt ctggacaaaa aaatacttta aagaacacct ttatctaaaa gtgagagata
     1921 gaaaaaagaa gcaaaggaat ggaacaaaag gaacatgaaa aattatatat gggactttga
     1981 tggtacattg tttgatacgt atcccgcaat ggtagatggt gcatggcaag cactaaaaga
     2041 ttttggtatc agtatggaca aaaaagaaat ctacttcaaa atgaaaaagt attctactag
     2101 ttatttgatc aatgaatcaa acttgaatgc cagagagttt aatgaattat ttcaccgata
     2161 tgaaaaagaa agtacggaag tttcccgtcc atttccagaa acgaaacaag tactggaaat
     2221 gctaaaagac aacggcggtc gtcatttcat cctgacccac cgtttgacag aatcaacatg
     2281 gggcctgtta aaagaacatc gattggcaca tctgattgag gaagtagtag ggatcgatca
     2341 ggactttcct cgtaaacctg atccagcatc tttaaattat ttgatcgata cttttcattt
     2401 agaaagaaca gacacgatga tgatcggcga tcgtcgttta gatatcgaag cagggaaaaa
     2461 tgcaggagtc gctacttgtc tttacgacat cgatcatttt ctgggtgaga tcccggcaga
     2521 ttatgtggta ggaaatctga atgaaatcct taccttgcag agcatcatta aaaagtagta
     2581 tcccctttca aaggagatat gttataattt tcataaagga gtgataaaat tatatggaaa
     2641 tcagaagaca aaaagaagtt gtagaagcat ttcattatga tatgagattg ccgaaccaag
     2701 aagtagaaac agatttgaaa gtaggcttct cgcctttaaa agaaacgatc gaaaactatc
     2761 cgaaagaaca tagcatcgta ggagcacgct tagagttccg tttagtgttt gatacatatg
     2821 ttttatctgg aagtgtgagt cagatcaatc atatcctcaa tcggaaaatt gaaggtcaaa
     2881 atgatgtcac acaagaagaa gtggacgagc tagtagcacc gttattcgat atcgtacgcc
     2941 gaatgtctta tgaggtgaca gagatcgcct tagatcagcc tggggtaaaa ttgaattttc
     3001 aatcagaaaa acaagaatag acagtaaaaa catacctgcg atagattaaa gctatcacag
     3061 gtatgttttt ttattactaa ttgtaagcaa aaagaatgtc atttcgataa aaatcgtcta
     3121 aaatataaaa gtcactgaaa aaaatttttt caggaggatg gttgaagaat cattctcggg
     3181 aacctttact tccttcgatg gacttgtcat taataatatg aggaggagaa aaatgacaac
     3241 actcatcaca gttttagtgg ttctagtttt tgcggcatta ttatatgtgt tctatcgtat
     3301 gcaaaagcgc catacaaaat tttcaacacg cgtctttaca gcattaggag ttggtatcgt
     3361 ctttggcggt ctgcttcaat tgatctttgg tgtagacgac aaagtcgtta ctcaatcaat
     3421 ggaatggatc gggattgtag gtcaaggata tgtatcattc ttgcaaatgc tagttatgcc
     3481 tttagtgttt atttcgatca ttggtgcatt cacaaaaatg aaagtttctg aaaagctagg
     3541 gaaaattagt gcgactgtat tgacttcttt acttggaaca acagctattg cagcattgat
     3601 cggaattggt tcaacaatgc tgttcggact ccaaggagct aaattcacac aaggagctgc
     3661 agaaacagca cgaatctctg aactggcaac acgtcaggaa acagttgaaa acttatcgat
     3721 tccacagcag attttatcct ttatccctaa aaacgttttt gctgatttag caggtagtcg
     3781 tgcaacaagt acgattggag tcgttatttt cgcggcattt gtcggaatcg cttatctagg
     3841 cgtgaaaaga aaagcgccaa aagaagcagc attcttttca gatttagtcg acagtctgta
     3901 taaaatcatt atgcggatcg tgacgcttgt tttgcgtttg acaccttatg gagtatttgc
     3961 tttgatgaca aacgttttgg caacaagtga ttttgcagct atcgtcaatt taggtaaatt
     4021 tgtcgttgct tcttacgtag cattgcttgc tgttttgatt gtccactcac tgattttgat
     4081 tggtgcaaaa gtcaatccaa tcaactattt caaaaaagcc tttccggtat tgagctttgc
     4141 gtttacttca cgttcaagtg ctggagcttt gccattgaat atcgagactc agacaaaagc
     4201 tttaggagta gatgatgcaa cagctaattt ctcagcaagt tttggtttgt ctattggaca
     4261 aaacggctgt gctggcgttt atccagctat gttggcaacg atcgtcgcac cagcagctgg
     4321 agtgaatgtc tttagtttgg aatttatttt gatgcttgtg gcaattgtaa caatcagttc
     4381 ttttggtgta gctggagtcg gtgggggcgc aacatttgct tcactgatcg ttttaggggc
     4441 aatgaacttg ccagtagcaa tcgttgggtt agttatctct gttgaaccat taatcgatat
     4501 ggcacgtaca gcagtaaacg tcagcggtag catggtagct ggtatcgtga caagtgcacg
     4561 catcaaagat ttagatcgcg atgtcatcaa tgatgattct aaagtgatcg atgtgaatgc
     4621 atagtcaaaa agaggttgtg atagaagtgg tcagctttaa gaaataagaa gaagtttcct
     4681 aaaaatagtt tctcaaattt ttgtgaaatt tcggcttatt tccgagaagc tacttctgaa
     4741 acaccgtgaa taagaggttg
//
LOCUS       DACCKU010000142         4758 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.147, whole genome shotgun sequence.
ACCESSION   DACCKU010000142 DACCKU010000000
VERSION     DACCKU010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.147"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            314..2158
                     /locus_tag="IVN13_002757"
     CDS             314..2158
                     /locus_tag="IVN13_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7013188.1"
                     /translation="MNYKQTAEEVLQYIGGEENISHLEHCSTRLRFTLKDNSKVNMSQ
                     LEKVDGVMGVRQNVQCQVIIGNDVVEVYDEVKALLGDNVSSTEEAPKQKQKLGSVLLD
                     FIISIFQPLIPAIAGGGVLKSLLLLASILGLISDQSQTYQILNLVGGAPLYFLPILVA
                     MTTANKLKVNQLVAVSAVGALILPEMTAMLAEGATFLSFGLDNIAYASQVFPAILTVL
                     FYAQLEKWFTKVSPKPIRIFFVPMMSLLITVPIALLLLGPLGYNVGTAFSSIIVSLYN
                     QFGWVATGVLAAVLPLMVVTGMHKAMIPYAVSSMSELGAELLYLPASLAHNISEAGAC
                     FAVSIKTKDKKLKSTAVSAGISALFGITEPALYGVTILHKRVLYAVMFSSLIGGAFAG
                     IVALQAFALVGPGIASITMFADKADPMNLVWAFAVSGISFVVAFAAALVLYKDKEVVN
                     EVETKMETAVVSDAVLSSPVNGKVISLSEVKDTVFSSGMVGDGVAIIPEEGILVAPED
                     GEITMVFETKHAIGLKTANGAELLFHIGIDTVQLEGKGFEAFVRSGEKVAKGQKLIAF
                     DIDLIKEAGFDPTVICVVTNKDNYTIRSFNLEKLVTNDSDLLAISPIA"
     gene            2213..3679
                     /locus_tag="IVN13_002758"
     CDS             2213..3679
                     /locus_tag="IVN13_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP7013189.1"
                     /translation="MTNTTSFPKGFLWGGAIAANQVEGAWNVDGKGLSVADMATYKPN
                     LDNKDFAAHMHVTLQSIKEASEDKSDKWYPKRRGVDFYHHYKEDLALFAEMGFKTLRL
                     SIAWTRLFPTGEELEPNEKGVAFYENVFKEMQRLGIEPIVTLSHYEMPLALAVKYNGW
                     VERRVIDDFVRFANVCFDRFGKYVKYWLTFNEIDSIHRHPFTTAGIIQEKSAEGQEAQ
                     DIYQALHHQFVASALVTKDAHEKIPGSQVGCMLTKLMTYPLTCAPEDVELTLKKNLEN
                     NFYADVQVKGEYPALIEKDLENRGIQIKMEDEDREILKKHTVDFVSFSYYMSMAESAD
                     PNAERTPGNTVLGVKNPHLPSTDWGWQIDPKGLKISLIELYDRYQVPLMIVENGMGAV
                     DQVVDGKIHDPYRIDYFKAHFQQMKEAIDEGVDLIAYTSWGPIDLVSAGTSQMSKRYG
                     FIYVDADDEGNGTYNRLKKDSFNWYKEVIETNGASLFE"
     gene            3716..4558
                     /locus_tag="IVN13_002759"
     CDS             3716..4558
                     /locus_tag="IVN13_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7013190.1"
                     /translation="MISIKKVLNSSVVLVEENGKEMIALGKGIGFGQKAGDEISGDKV
                     DKIFLPFEEKKSSQLLELVEEIPIEFFEITRTIVFEAEKGLDKKLNPGVYLTLTDHLH
                     FAVERAQKGVNIANRLYWEIKIYYPKEFQIGKYALTLLNEQYGIDLPKEEASNIAFHL
                     INAQSEDPDSGAGFKYAKMIGGIVNLVRYSLQKDIDTNSIHYARFITHVRFFVERYYS
                     DALLDDGEEEFYNQMWILYPSAMESAAKIKDYIEKVYHKTIPNDEVVYLGVHINRLMK
                     HANG"
BASE COUNT         1555 a          764 c         1021 g         1418 t
ORIGIN      
        1 ttggaagata ttcttagaaa aaagacaaaa cttttagaac acgaaaacta tattgaacga
       61 ataaaatgaa aaaatgatta aaaaatcatt ttattattta cgtttacatt ctaatatgtt
      121 aaattgaata taacttaaac aggcatgtga ctggtaatgc aggcaggacc taaataagtt
      181 gaataacaat gtatgcgttt caatacacgt ttgccttgtt gtcaatttat ttagtttctg
      241 cctgtttttt gtgttgagga tggccacttc gactcaaaag ttcatcactc aaaaaagaaa
      301 agagggaaac accatgaatt acaaacaaac agcagaagaa gtattgcagt acatcggtgg
      361 ggaagaaaac atatcccatt tggagcattg ctcaactcgt ttacgtttta cgttaaaaga
      421 caacagcaag gtaaatatga gccagttaga aaaagtggat ggcgttatgg gcgttcgcca
      481 aaatgtgcaa tgccaagtaa tcattggaaa cgatgttgtt gaagtgtatg atgaagtgaa
      541 agcgttatta ggtgacaatg tttcaagtac tgaagaagca ccaaaacaaa aacaaaaatt
      601 aggttcagtt ctattagatt ttattatttc tattttccaa cctttaattc ctgcgattgc
      661 aggtggagga gtattgaagt ccttgttgct actagcttct atcttagggc tgattagtga
      721 ccaatctcaa acctatcaga ttttaaattt ggtcggcggg gcaccgcttt atttcttgcc
      781 tattctggtg gcaatgacta cggctaataa attgaaagtc aatcaattgg ttgcagtatc
      841 tgctgtcggt gcattgatcc ttcctgaaat gacagctatg ttggcggaag gtgctacatt
      901 cttatctttt ggattggata atatagcgta tgcatctcaa gtattcccag ccatcttaac
      961 tgttttattt tatgctcaac ttgaaaaatg gtttacaaaa gtttcaccaa aaccaattcg
     1021 tattttcttt gtacctatga tgagtttatt gattacagtt cctattgctt tgttgcttct
     1081 aggaccatta ggttataatg taggaacagc tttctcttct atcattgtgt ctttgtacaa
     1141 tcaatttgga tgggttgcaa ccggagtctt agcagctgta ctgccattaa tggttgtaac
     1201 gggtatgcat aaagcaatga tcccttacgc tgtttcatcc atgagtgaat tgggtgcaga
     1261 acttctttat ctcccggctt cattggcaca caatatttca gaagctggcg catgctttgc
     1321 tgtcagtatc aaaacaaaag acaaaaaatt gaaatctacg gctgtttctg cgggtatatc
     1381 tgcattattc ggaattactg aaccagctct ttacggggta acgattctgc acaaacgtgt
     1441 tttatatgcc gttatgtttt ctagcctgat cgggggagca tttgcaggaa tcgttgcact
     1501 tcaagccttt gctttagtgg gtcctgggat tgcaagtatc acgatgttcg ctgacaaagc
     1561 tgacccaatg aatctggttt gggcatttgc agtatcgggg atttcatttg tggttgcttt
     1621 tgctgcagcg ttggttcttt ataaagataa agaagttgtc aatgaagttg aaactaagat
     1681 ggaaacggcc gttgtttcag atgctgttct ttcaagtccc gtgaatggaa aagtaatctc
     1741 actatcagaa gtaaaagaca ctgttttttc tagtggtatg gtaggtgatg gagtagcaat
     1801 cattcctgaa gaagggatat tagtagctcc tgaagatgga gaaatcacaa tggtatttga
     1861 aacgaagcat gctattggtt taaaaacggc taatggtgca gaacttcttt ttcatattgg
     1921 tattgatact gttcagttag aaggaaaagg gtttgaggca tttgttcgtt ctggtgaaaa
     1981 agtagccaaa ggacaaaagc tgattgcatt tgatattgat ttaatcaaag aagcaggatt
     2041 cgaccccaca gttatctgtg tggtaaccaa taaggataat tacactatcc gttcatttaa
     2101 cctagaaaaa ttggtaacca atgatagtga tctcttagct atcagtccga tagcttaaac
     2161 acaagatagt gaaaaaaata tattgaatga aggaatagag gagaatagaa taatgacaaa
     2221 cacaacaagt tttccaaaag gatttttatg gggcggcgcg atagcagcaa atcaagtaga
     2281 aggtgcttgg aatgtagacg gtaaaggtct ttcagtagcg gatatggcta cttataaacc
     2341 aaatttagac aacaaagatt ttgctgctca tatgcatgtc accctgcagt ctataaaaga
     2401 agctagtgaa gacaaatctg ataaatggta cccaaaaaga aggggcgttg atttttatca
     2461 tcactataaa gaagatttag ctttatttgc cgaaatgggc tttaaaacct tgcgcctctc
     2521 tattgcttgg actcgcttgt tccctacagg agaagagctg gaaccgaatg aaaaaggggt
     2581 tgcattttat gaaaacgtct ttaaagaaat gcaacgttta ggaattgagc cgattgtgac
     2641 actatctcat tatgaaatgc cattagcttt agcagttaaa tataatggct gggtggagcg
     2701 tcgagtaatt gatgattttg ttcgcttcgc taacgtttgt tttgatcgct tcggcaaata
     2761 cgtaaaatat tggctgacat ttaatgagat agacagcatc caccgtcatc catttacaac
     2821 agctggaatt atccaagaga aaagcgcaga aggtcaagaa gctcaagata tctatcaagc
     2881 attgcatcat caatttgttg cgagtgcgtt agtgacaaaa gatgctcatg aaaaaattcc
     2941 aggaagccaa gttggttgta tgttaacgaa attgatgact tatccattaa cttgcgcacc
     3001 agaagacgtt gagctgacct tgaaaaagaa cttagaaaat aatttttatg cagatgttca
     3061 agttaaggga gaatatcctg ctctaatcga aaaggatttg gaaaatcggg gaattcagat
     3121 taaaatggaa gatgaagatc gagaaatttt aaagaaacat accgtagatt ttgtttcttt
     3181 cagctattac atgtccatgg cagaatcagc tgatccaaac gcagaacgca caccgggaaa
     3241 tactgtttta ggggttaaga atcctcattt gccttcaaca gattggggct ggcaaatcga
     3301 tccaaaaggt ttgaaaattt cgttgatcga actttatgat cgttaccaag taccgttgat
     3361 gattgtggaa aatggtatgg gagcagtcga tcaggtagta gatggtaaaa tccatgatcc
     3421 gtaccgcatc gactacttta aagcacactt ccaacaaatg aaagaagcaa tcgatgaagg
     3481 tgtagatttg atagcttata ctagctgggg accaattgat ttagttagtg caggaacttc
     3541 tcaaatgtct aaacgatatg gatttattta tgtggatgca gacgatgaag gaaatgggac
     3601 atataaccgc ttgaaaaagg attcgttcaa ctggtacaaa gaagtgatcg aaacaaacgg
     3661 tgcatcctta ttcgagtagt ctcgatcttt ttaagcagcg atagggggat aatgagtgat
     3721 ttcaataaaa aaagtattga attcaagtgt ggtactggtt gaagaaaatg gcaaagaaat
     3781 gattgcatta ggtaaaggga taggctttgg acagaaagct ggggatgaaa tatcaggaga
     3841 taaagtagat aaaatatttc tgccttttga agaaaagaaa tcttcacaac tattagagtt
     3901 agttgaagaa atacctattg aattttttga aatcactcgt actatcgttt ttgaagcaga
     3961 aaaaggatta gataaaaaac tgaatcctgg agtctattta accttaacag accacctgca
     4021 ttttgcagtt gaacgcgcac aaaaaggggt aaatattgca aaccgtttgt actgggaaat
     4081 aaaaatctac tatcccaaag aatttcaaat tgggaagtac gcactcaccc ttttgaacga
     4141 gcaatatgga attgatcttc ctaaagagga agcctcaaac attgctttcc atttgatcaa
     4201 tgcacaatcg gaagatcctg atagcggcgc tggattcaag tatgcaaaaa tgatcggcgg
     4261 gatcgtcaac ttggtccggt attcgttgca gaaagatatc gatactaatt cgatccatta
     4321 tgcccgcttc attacacatg tacgtttctt tgtagaaaga tattattctg atgctttgtt
     4381 ggatgatggt gaggaagaat tctacaatca aatgtggatc ttatacccgt ctgccatgga
     4441 aagcgcagct aagatcaaag actacattga aaaagtttat cataaaacaa ttccgaacga
     4501 tgaagttgta tacctaggtg tccacatcaa tcggttgatg aaacatgcta atgggtgagc
     4561 gctcagtaat actattgatg cttttaaaat ctttagagat ggattatagt aaaaacaagg
     4621 tgatttccag ctgtttgtta tctataagag acatttaaaa aggattaacg taagataata
     4681 aaaaatctcg gctctttgtc aaatgatgtt ggtaaagaga aatcttagaa taatttggac
     4741 agaacagaaa gaagaaaa
//
LOCUS       DACCKU010000143         4544 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.148, whole genome shotgun sequence.
ACCESSION   DACCKU010000143 DACCKU010000000
VERSION     DACCKU010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.148"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(503..844)
                     /locus_tag="IVN13_002760"
     CDS             complement(503..844)
                     /locus_tag="IVN13_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP7013191.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     KKSDQIKRVYFPIAEWEKAGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSASDTKKL
                     NRFIEKLQRKV"
     gene            complement(834..1115)
                     /locus_tag="IVN13_002761"
     CDS             complement(834..1115)
                     /locus_tag="IVN13_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP7013192.1"
                     /translation="MKVKNPTEKSRIQVGIDKNLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTKDMPVDVLDTDEKLEEWFDEA"
     gene            complement(1486..1725)
                     /locus_tag="IVN13_002762"
     CDS             complement(1486..1725)
                     /locus_tag="IVN13_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013193.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEDLLRKTQRKGYPDKILKQIGYYM
                     PEERIEKEFCEVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(1745..1993)
                     /locus_tag="IVN13_002763"
     CDS             complement(1745..1993)
                     /locus_tag="IVN13_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013194.1"
                     /translation="MEGMDFLDHEDLVDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDDTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2008..2574)
                     /locus_tag="IVN13_002764"
     CDS             complement(2008..2574)
                     /locus_tag="IVN13_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013195.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMEKENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGADDCVAYYTTAE"
     gene            complement(2575..3120)
                     /locus_tag="IVN13_002765"
     CDS             complement(2575..3120)
                     /locus_tag="IVN13_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013196.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3146..3913)
                     /locus_tag="IVN13_002766"
     CDS             complement(3146..3913)
                     /locus_tag="IVN13_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAP7013197.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVES
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
BASE COUNT         1290 a          953 c          594 g         1707 t
ORIGIN      
        1 acgcgaccgt tcgttcgtct tttcctccgt gggactcacc caccgcaagc ggttggttga
       61 gttgtggctc ctattcaaaa ttcactaatc tgttattttt tatttctatt caaataggta
      121 acgttcgttt gcaagcaaac tctctctatg taacttgcca gttacatcag cggtaacgct
      181 acgtcgcaag cgacttgctc taatgaaact gccgtttcat tctgcctgtt cgttcgtagc
      241 aagctacttc actcaccgta tccgtctgaa ttattgttat ttattgctat tcaatatccg
      301 tcagacattt tatttattta catacatttt atataaaata tatattataa ttcgtatatt
      361 actaaatatc attatttaac aattcttaac gttttattaa tgtttataac cacgtatata
      421 tttacgtata ttttcatata cataaatacg tttatataaa cgtatatctt aatatacata
      481 taaacaaaaa agaaaagcct tgttagactt ttctttgtaa tttctcgata aacctattta
      541 gctttttcgt atcactagct gataacatac caaccttttt gaatgatatg ttctctcggc
      601 ttttgggaat agcctttaaa gaccctacgt caatccatga ttgcttttta agtcctgctt
      661 tttcccattc tgcaatagga aaatacaccc tctttatttg gtctgatttt ttctcatatt
      721 gacttgtgat tccaaagaat tctactctat cttctttatc actaaccacc aaaataggtc
      781 ttcttttacc accattttta tctgtaaatg caacatatac cgttactatc tcattaggct
      841 tcatcgaacc actcttctaa cttttcatct gtatctagaa catctactgg catgtcctta
      901 gttagctgtt gcaatctgat tccttgcttc tcctcttcac ttagctttat ctctactgga
      961 aactcacctc tagcgactac ttgtttgtaa agaattgtta ttgctgttgt aggatttaaa
     1021 cctagctctt ccaatatcat ttctgcgttc tcttttaaat tcttgtctat tccgacttgg
     1081 attctgcttt tttcagttgg atttttgacc ttcatttcat ccactccttt tgagtataaa
     1141 acactcatta tttatcaaat tgtccacatt tatgattata ttatactctt aataaactac
     1201 attgcaagta atctaatcaa attaagatgc tatatcatac ggtaattgaa caaattgtcg
     1261 ctaacccaca catacggaat cgtaatgaat gaaaaaaaga caagcctatt cgcagtaggc
     1321 ttgtctttgt gcttctcttg tacaaaatct tcacatttgt aaattaattt tagcaccttt
     1381 gttaggaaag aacaacttaa atgagatgct aaagaattaa tagttctcaa tctagcatat
     1441 tcacttaata ccatcttatc ttcattcatg gttcaccacc tcatcttaat tattttcaaa
     1501 ttggcaaatc aatttatttt gggttaacaa aactaatagc tgttcactat caaaaagaac
     1561 ctcacaaaat tctttttcta tcctctcttc tggcatgtaa taacctattt gtttcaatat
     1621 cttatctgga taaccttttc gttgggtttt tctcaataaa tcttcatcat ttccaacata
     1681 ataataagaa caccattgca tacccttact tttaaatttt gtcatgtttc tatcttcctt
     1741 tcacttattg acccaaccat gaaaaaatat attttgctaa atcttccttt tctattccat
     1801 ataaaacatt ctctaaatcc aatttatatt cctcgtcaac aaggctttct gtatcgtcat
     1861 cataaagaac atatactgca taatttcttt cgtaaaaggc attagcaaga cttctgctaa
     1921 tacctaccat tcctttccat gtataaccaa aatcaaccaa atcttcatga tctagaaaat
     1981 ccattccttc catgctgatt cacactccta ttctgctgtt gtgtaatatg caacgcaatc
     2041 atctgcccct actaaccaca cacaccaatc ttcaatttca tcatcgcttc tcttactcaa
     2101 ttcctctttg gtacaaaaaa ctacttcacc attttctttt tccatctcat aatcttgtag
     2161 catgtattca aaccaacttc tttcggttgg aaattctatc tcgtggcaac tctggcaaat
     2221 aaaatcacca tattcattga ttcggtagca accttcatct tctacaaatt catgattaca
     2281 acaaacacat ctagtacctg ttaggacttc cccattaatc gttgttctaa cggtatcttc
     2341 ccttgctacg tgttcactgc tgacaagcca atcaatgatt ttctttgcca attcttcatc
     2401 agaatcggca aagcctttaa acgtgtttct ctttccttga aagtagtatt tgcaacttgc
     2461 ggtagtttct atggttccat aacttttttg gaataattca atcatttcac catcaatcac
     2521 tttttgactt ttcgcttcta aattagggaa ctggtctttt cttttttcaa acacttattt
     2581 cacgctctct tcttctaggt ttattccata attttctagc aataatctgt atcccttaat
     2641 aaaatctttc gtagcaaact cttttccgct ttcacaaaac ttgtttagtc ctatctgttg
     2701 accctcaaaa tcaaacaatc gcaatctggt tttatcatct agactaaatc cattttcatc
     2761 gaacaccatc tctaaatgag catagccatc attgttatct gaaaacttca tcacatcttc
     2821 aatcgtttct tgataaggtt catagcgccc accaaaccct gtttcatcat aagcactcac
     2881 tccagattca taggtaacaa aattgataac tcctgtactt ctatcaaaga ttccttttgc
     2941 taattcttct cttgatttat ctcgtaagtc gtatgcttta ttgattaaat aaattccttg
     3001 attcaatacg tccatcagca tatatcgacc atacccccac tgataatgaa tcgacacgct
     3061 tgctttcgtt ttgctgttac catatttgac tgtaattaac gcttgtgttc tttcgcccat
     3121 cttgttttcc tcttttctct aatttttata aagaaccatt ttcttgaaat gagtaatagc
     3181 ttgtgttttt acttgttacg atgatatggt ctaacaattc aataccaatt gtttttcctg
     3241 cgtttttcaa attcgtagta aaacttttgt cattttcact tggaaattca tatccgctcg
     3301 gatgattatg tgatactgcg atttttgctg aattgtttag caaagctagt tgaaaaatct
     3361 ctcttggtat cgctactgat tgattaattc cacctttgaa aacttcgcta taagcaatga
     3421 cctcattctt tgtattcaga cacatcacta gcaaagattc actagcattg tttccaatta
     3481 agttagtagc aaagtttgct agaccaaccg agcttgtgac tctataaaaa cttctatctt
     3541 cactgatata tttttgttcg attttcaaga cattcttaat caatccttca ccttcattct
     3601 cggtcttgct ttcaacttca atagattcaa gactgctatt tcccatatca gaacccaaca
     3661 aagcatttct gaaatccaat accttttcaa agttatcagt cataaccatt tcataggtgc
     3721 tattccattc attcaaatat ttctctttga tgagaatgaa cgctctcggt ttacttctgc
     3781 caattctgat attttgtgta tcgtgaaaaa tatagttatc aagtaaagta ccatcgtact
     3841 tttcatagtt cccactgata tttgttaact tgtttaccat ttcttgatta acttctaaaa
     3901 ttctatctct catgttgaac gcttctttct gtttttgatt tttggttttt caaaattctt
     3961 ttttgtttgg attgttttag agggaggtgg ttttttcttc atcgctattt ggttttgcat
     4021 tttatgtttc ttcctttcgt tcatttattt atctttttcg cctgtcttgc cttgtcggga
     4081 gtgattagaa tgcgttctta tctgccggat taatacaagt gtttaagtaa attattttcg
     4141 taacgcttgc gtttagaaaa aaatttacgc cacttgtatt aatccaaaga tgagataagc
     4201 tctaaacact tctgacaagg tatgacaggc gaaaaagata atgggcgtaa ggaattataa
     4261 atggcaaaac caaataaaga gcgatgaatt tcaatgcttt caggcgtagc ttgaaagcct
     4321 aaaagtcttg aagaatgaaa gactttttac cttcaaagca gacctagtaa tcttaaatag
     4381 gcgatggcta tttaagatta cggtgttgac ccactaaata aacagaaatg gaaccgatca
     4441 agcaaaatcg gactaggcga tggctaggac ggtttgcggt ggctttagga aacgttcatg
     4501 gtgagatgag catatctagc aatgtcgggc taagctatgc ttag
//
LOCUS       DACCKU010000144         4390 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.149, whole genome shotgun sequence.
ACCESSION   DACCKU010000144 DACCKU010000000
VERSION     DACCKU010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.149"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..323
                     /locus_tag="IVN13_002767"
     CDS             <1..323
                     /locus_tag="IVN13_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013198.1"
                     /translation="VSNGETKTIDQQTGVFEFSVEVQANQKQAYYIVNESSPENISNS
                     EDILLLTPVSDKNGEFLKDVWIYPKSEASQPKEEVKKIVSTGVKKNFFENCWDFVTHL
                     FSRD"
     gene            336..596
                     /locus_tag="IVN13_002768"
     CDS             336..596
                     /locus_tag="IVN13_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013199.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            593..2683
                     /locus_tag="IVN13_002769"
     CDS             593..2683
                     /locus_tag="IVN13_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7013200.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            2710..3174
                     /locus_tag="IVN13_002770"
     CDS             2710..3174
                     /locus_tag="IVN13_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013201.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            3171..3413
                     /locus_tag="IVN13_002771"
     CDS             3171..3413
                     /locus_tag="IVN13_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013202.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            3431..3733
                     /locus_tag="IVN13_002772"
     CDS             3431..3733
                     /locus_tag="IVN13_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013203.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            3803..>4390
                     /locus_tag="IVN13_002773"
     CDS             3803..>4390
                     /locus_tag="IVN13_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013204.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEV"
BASE COUNT         1616 a          671 c          877 g         1226 t
ORIGIN      
        1 tagtttctaa tggagaaaca aagacaattg atcaacaaac aggtgtcttt gaattctcgg
       61 tagaagtaca ggcaaatcag aaacaagctt attatattgt caatgaatca agtcctgaaa
      121 acatttctaa tagcgaggat attttgctat taacacctgt ttctgataaa aacggtgaat
      181 tcttaaagga tgtgtggatc tatcctaaat cagaagcttc gcaacctaaa gaagaagtaa
      241 aaaaaatcgt ttctactggc gtaaagaaaa acttctttga gaattgttgg gattttgtta
      301 ctcacttgtt ttctagagat taaggaagaa ataaaatgaa actgatcaaa ttattattat
      361 tcaatttttc ttgtttaacc gcagtctttt tgtactgcgg tttattattt tctaaactat
      421 ctattttgcc aagctaccaa ctatacctaa tgaggatttt aaacatacca gctgcttcca
      481 tgccgctaga tattatggaa aagctcatta ggaatccatt atttactacg atcaatttga
      541 tcgttattgt cggaatttca ctagtcattt ataaaaaaga ggggatgaag ggatgaattt
      601 taaacactcg tggacaagac ggttaaaata tgtttttccc ataatgatga tgttaggtac
      661 attgtttggg ttaaaaacag ccaatgtctt tgctgaaaaa gttattgtcg atccgtcaaa
      721 tccaatccag aacgtaaaga ataattttat tattccaaaa gatgttccta atgcaaaaac
      781 aaatattact gtaaacggag cgtcaaaagt ttacaaatat catgttgatg ccaacaaagg
      841 tggttttaca gacgactacg taggattagt ggagggaaac aaaaatatac gctatccttc
      901 tttccaaaaa gaatacggtg aaacaaacct tgttttagaa ggcgtaacaa ccaacaccaa
      961 agtcaatatt gactatggaa aaatcggtac gtataacggg aaaaaagtca atatcaagct
     1021 agttctatct aatattcact tatactcgga tacgttgccg tggaatctac tagataacaa
     1081 ctatacaaaa actcattttc gagacgatgg ctataaaaat acgaatggtg caatgagtaa
     1141 atctaaaaaa agaacagtac tttggatctc agacaatctt ttctcaggaa ttgtttacca
     1201 ttcaacacaa atgaatgtgc agctagtagc cacatatgag gacggtagcc ccgttcaatt
     1261 ttcaggagat acatttatca gttttaactc tttgaaccct gcaggaggaa aaagcacgga
     1321 tcttaaagga gaatatgccc actatgacaa aatgaatacg acagattggt atgtcagaaa
     1381 agatacggtt ctttcagaat ttaaatcttt ctacaacaat ctgaacgtgg taggtggcca
     1441 cccaggagga agctctaagc tgacacaagc ggataatgat ttcaataatt tgcatgataa
     1501 attaggagat ccgaagtttg gtcaaggtac tgtgtccttt aaaatatctg aagcaaatcc
     1561 gacgtttgtg attggttctt ctaatgttca aacctggttt actttatctt ctgcgacaat
     1621 ctttagtgtt gttcctgatc aacctgaaaa aacaggggtt gataaaaatg gaaataacgt
     1681 gaacgataaa atgttgcaag taggagacac gattcaatat cgaatcaaac agaaggtgaa
     1741 tcgattagga gtggatttac tagcggttta cgatcgattt gagttaatcg ataatttacc
     1801 aaaagaagta aattacgtcg atgcacacgt tgaaagtggt acaaataaaa aattcgatgt
     1861 atctggtgaa gtgacctatg acaaaacaaa acaccaggta aaatatgcag cgaaagctga
     1921 tacattaaaa aatcgaatga agtataacgg cgaaacttac gaattagtga tcaatgtaaa
     1981 agtcaatgaa ttggccaatc aaaatagtgt agccaaaaat cagggaacgt cgatcattaa
     2041 taaagtagaa aaggatacaa atatcattac cgtttatttc cctaaaattc cagtgaaaga
     2101 agtacaacaa aatggaaaag atgtaaacgg acgaaatgac ggtaagaagg gtacgccgac
     2161 agctccttta aacgcaggat ctgaagtaca atatctagtc acacaaaaat ggcacacaaa
     2221 aggcgtagac gctgcgtcag atcactacaa acaatttagt attcaagatc caattgaagc
     2281 tcgtttgaca tataaagaag gttctgcgca agtaatagat aagagtaaag gaaaagatat
     2341 tacttctgaa ggcacactaa cctatgatag caattcaaga acgctgaagt gggaagcttc
     2401 cgctgacttc ttaagtaaca atcttttaga tggacgagaa attcagctga tatttacagc
     2461 taaaactcca ttacaatcag aaaaaaatat tgataaccaa gccgtagtag cagtagataa
     2521 tgtttctaat aaaacaaatg ttgtgacaat tggggttgat cctaacttgc cacaggtcat
     2581 tgttcctaaa acaggttcta cacatttagt aacgatttca gcggtcagct tactattact
     2641 tgtgttagca acttttagtt atgcggttct gagatacatt taaaataaaa aaacgaaaga
     2701 atgaataaaa tgaaaaagtt aataatagta gttacttcct tgattgccat gattgttatt
     2761 ttagtcggat gtagtaatga aaaaaataaa caaactaatc aagataatgg cgtattgaaa
     2821 tcaggaacta tgtggaaaga agaagtaggt ggtcttgttt ataatttgaa aattatagac
     2881 gagactacct gggaatactc tgagagtgta tggcatccag atcctgtaca aataacagtc
     2941 aagaggcaaa aagactataa aggtttagaa agatacaaaa tagtagattc agctggtgtt
     3001 agggaattta taaacaaatc tgattcttta tttattgttg ttccctatga gaagaatggc
     3061 gtaaaaaaaa taatttttct tggatctagc aaggatgaaa agcgaacgaa agagaaattg
     3121 attcacgatg gatcacaatc caataaatat aagttacaaa agactagcga gtgattgcta
     3181 gtctttttat ttgtcctgaa ggaggaatca cagtggcaaa agctaaaacg ccatttgaaa
     3241 aagccgcgtc taaaatctta gaacgccgcg gtgtatctga aacacagtgg aaacaagaag
     3301 tccttgaaaa agcacaattt gacctattac ggggaaaaga caaggactta gaaggatata
     3361 tctatcaacg tgaacgtgaa aagcttgttt tcgatgaaat tattaaacac taaaaggagc
     3421 gaacaatttt atgtttgaat caattacaat gaatttttta acacaaccga aagtagtagc
     3481 tgcggttgat atttttagag cattacaaac tgccgcaact agtttaaaaa acaatctgat
     3541 gatttttgct gctgctgcgg ccgttgcttc cattgttatt gggggtcttt tcttcctatt
     3601 tggacgaaga ggagctgaaa atgggaagat gcacgtaggc tatgtattga tgggggtagg
     3661 agttatttta gcagcagctt ctattgtggt ttcgttattt ggattgtttg gacaaacacc
     3721 tcaaggatta taagaaagta aaaaagtagg agtaagaagt agtcgatact ttttactcct
     3781 tttttatgaa aggagaatac tcatgtactt tacgattgca ggcaatacca aaacagataa
     3841 agagttaata aaaaaagcaa cgaaggaagt aggaaagcgt acttcttttt taccgaaaac
     3901 aattaaatat tctgtcattg aaacggaaga acagctacat gtgttaggga aagtactgga
     3961 acacgaaagt cgatcttctg ttaagttgac tctcacgtta catgacacag aaggaaaaat
     4021 agagaaagct ggtacagaat acgtttttgg cccactggat tatcaaattt cagaaggttt
     4081 ctatcctaca ttgatggatt taatccgcct tgatgtgttt aaagaaacgg atgaaactat
     4141 gagtgtggaa gagtcttatc gaaacgaaaa acttttacaa gttttagaag aagagttaga
     4201 gagcgaccaa agaaaagtga atcaagggag tgaagtcgaa gtaaatgaag aagtaccatg
     4261 ggaacgacca atatttccaa agaaggagtc aaaagaaaaa gagacagtct atgttgacga
     4321 acaagctgat attccgactc agaaatcgat atttgaggaa gagaacgatg tgatgggtca
     4381 attggaggta
//
LOCUS       DACCKU010000145         4319 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.150, whole genome shotgun sequence.
ACCESSION   DACCKU010000145 DACCKU010000000
VERSION     DACCKU010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.150"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(293..838)
                     /locus_tag="IVN13_002774"
     CDS             complement(293..838)
                     /locus_tag="IVN13_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013205.1"
                     /translation="MIYAGVVCFGIAGNYIMNMFHLNKYVNFTIRNIKLKQHLKPIMV
                     LFVSVIAIELYSLIDVTMLTHMTSSARVGYLSNAVKIVKMIANTFTAMGQFSYQDLVF
                     ITLRRIPLNWKKLYIIFFEIMIVIVAEHIVLNLLGESFISAVRTLQVLAPLVVMMPLS
                     GGVFGQLLLTTDSEKIFYDVL"
     gene            complement(1298..1687)
                     /locus_tag="IVN13_002775"
     CDS             complement(1298..1687)
                     /locus_tag="IVN13_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013206.1"
                     /translation="MYDTIITIFSTERIWGYGANNHHIVLYSLVKAPNAQNGILNCLI
                     QFGIAGTLLLLITLHMMLRKGKNINHSVSIMALIMMMSVISSVEITIDIYFILFCAII
                     WAYQFKAKHYKNKEVKDEKPPCKICCF"
     gene            complement(1773..2151)
                     /locus_tag="IVN13_002776"
                     /pseudo
     CDS             complement(1773..2151)
                     /locus_tag="IVN13_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038005.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(2308..2604)
                     /locus_tag="IVN13_002777"
     CDS             complement(2308..2604)
                     /locus_tag="IVN13_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013207.1"
                     /translation="MDWLEQSDEGWVFGSRISEGMAAEIAKANELGKVVSNLYQPDSD
                     AHECGGCDCCHEKFDIDQKMAEMHDHLLHSICERTGLSEEEVDQKLMEISEVML"
     gene            complement(2672..3277)
                     /locus_tag="IVN13_002778"
     CDS             complement(2672..3277)
                     /locus_tag="IVN13_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP7013208.1"
                     /translation="MIKVISSIVVNVLTALYQPFGFSVSLSILAMYFYLFATAKGVAG
                     QGFKKSLEVWILCFRSSAKFRRLFFLVFYTTMILFRTLLNRNLWVNPLSDVMGGWWIY
                     ETNPSTGEVNLTTECIENLILFIPFSALLMWFMEQKEKIKCILWTSTKVVFLFSVSIE
                     FFQLFLRLGTFQLSDLFYNTLGGFLGGLIYWIFYKVNKRFS"
     gene            complement(3398..4064)
                     /locus_tag="IVN13_002779"
                     /pseudo
     CDS             complement(3398..4064)
                     /locus_tag="IVN13_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005603388.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine pyrophosphorylase"
     gene            complement(4076..4309)
                     /locus_tag="IVN13_002780"
     CDS             complement(4076..4309)
                     /locus_tag="IVN13_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP7013209.1"
                     /translation="MLVNVRVKDKTEAQNDPDMQNAVKAVADKLGNSGRILVRESGTE
                     LVVRVMVEAATEEICKQYVDSVVSVIKKNGYAV"
BASE COUNT         1412 a          965 c          683 g         1259 t
ORIGIN      
        1 ctttctgatt gggtttggtc actttaatct tacagggatg agtgcttttt gtgtacctta
       61 aaatcaaaaa aaatggggca ataatcagat ttttttctga ctattgtccc actctctttt
      121 tattactctt catcttctac tattccacta attaattagt tgacagatac aatgctttca
      181 catcctgcac gaacaaactc gagctctgaa ccaatcactt gcgagttgat aaattcaatg
      241 accatcagaa taattaaaat agcattaaaa ataacattac aaatacttcc tgttacaaca
      301 catcgtaaaa aattttttca ctatcagttg ttaaaagaag ctgaccaaat acaccgcctg
      361 aaagaggcat cataacaacc aatggagcca acacttgtaa agttcgtaca gcggatataa
      421 aactttctcc caataaattt aagacaatat gctccgcaac tatcacaatc attatttcaa
      481 aaaaaattat gtatagtttc ttccagttta aaggaattct tcttagcgta ataaagacta
      541 agtcttggta agagaactgc cccattgctg taaaagtatt agctatcatc tttacaattt
      601 tcacagcatt cgaaagatat cctacacgag cagatgatgt catatgagta agcattgtta
      661 catctattaa actgtacaat tcgatagcta taactgatac aaaaagaacc attattggtt
      721 taagatgttg ttttaattta atatttctta ttgtaaaatt cacatattta ttcagatgga
      781 acatattcat aatatagttt cctgctatac caaaacacac tactcctgca taaatcacaa
      841 tatccgtctt tttttaacaa aaagaagcat aaaaatcaac gatacaagct tgataataaa
      901 acttctaact gttatatatt tatattcttc gaatccttgg taaacccatt caatattaaa
      961 ataatttaga acaattaaaa aaaatcaaag aaagttgatt atatcccgaa ataaatacaa
     1021 cagacaaata tatgagtaaa cttatagtag ttgcaaatgc attaattata aataatcaga
     1081 gaaaattata tctgtaaata ttccatcgct ctgggctctt tttttgaaat aactttcaca
     1141 ctatacgttg gaatacccaa ggaagcagcc attgtaaaaa taggtcacag aattctgtac
     1201 tgatgtaact taaccaattc cttcagcttc taaaatatgt gaaacatacg atactgttat
     1261 aagaggaaaa acgactgtca aagctttata tagcatgtta aaaacagcat attttgcaag
     1321 ggggtttttc atctttgacc tccttattct tgtagtgttt cgctttaaat tgatatgccc
     1381 aaataatagc acaaaataga ataaaataaa tgtcaatagt aatttcaacc gacgaaatta
     1441 ccgacatcat catgattaat gccattattg aaacagaatg atttatattt tttcctttac
     1501 gtagcatcat atgtaatgta atcagcaata acaatgtgcc agcaattcca aattgaatta
     1561 aacagtttaa aataccattc tgtgcattag gagcttttac tagcgaatat agaacaatat
     1621 gatgattatt tgctccataa ccccaaatac gttctgtaga aaatatcgta attatcgtat
     1681 catacatttg caaacgacca tgcagatcaa gagtttcacc taaaatcttc gtgataatat
     1741 actaaaccgc aggttgagat aatattacag catcaaagaa caacaaaact gtatctgcta
     1801 gcgtattcca tactctggac catgtgcgga tgcacacgga agattcggca aatctcctcc
     1861 acatcaaatt ggcaagtaga aagaaactga ctgtcttcgg gtggaagaga gatcgtcttg
     1921 taactcattc cctcttcaag gactgcaccc gatgggcatt ggcagcaccg cctaccagtt
     1981 atctctcact cttgccgggt ctttcagaac accccgatgc tctaacacat cactcggcta
     2041 ggctccgttt ttgaagaagg aagccccata tcgttccacg acatcttcgg ccagcttaaa
     2101 gcttaagaag ctcctccatc tgttgggaga caccttcaca tatatggtca aagcccataa
     2161 agacctccgc cagactgtcc atgaccttct tcagtgctgc gatcatctgc tctctcttct
     2221 ccatggctta cacctactcg acagaatcag cgacatcaga ggcaggagca tccttcatgt
     2281 gctatgccag aaactcatcc agctggttca tagcatgacc tcgctgattt ccatcagctt
     2341 ctggtccacc tcttcctcag acaggccggt gcgctcacag atggaatgga gcagatgatc
     2401 gtgcatctct gccatcttct ggtctatgtc gaatttttcg tggcagcaat cacagccgcc
     2461 gcactcatgt gcatcggaat caggctggta aaggttactg acaaccttac ccagttcgtt
     2521 ggctttggca atctctgccg ccatgccttc ggaaatacgg cttccaaaca cccacccctc
     2581 atcagactgt tccaaccagt ccatagcgaa agtaggtcct cttccagctc atcgtggagg
     2641 aactgcgtaa aatacaggtg cattcctctt ttcacgaaaa ccttttgttg actttataga
     2701 aaatccagta gatcaatccg ccaaggaaac caccgagggt attgtagaac aagtctgaaa
     2761 gctggaaagt tcctaaccgt aggaagagct gaaaaaattc aatggaaact gaaaacagga
     2821 acacaacctt ggtacttgtc cagaggatac acttaatctt ctctttctgc tccataaacc
     2881 acataagtag tgcggaaaat gggatgaaaa gaattagatt ctcgatgcat tcagttgtta
     2941 gattaacttc accggtagaa ggatttgttt cgtagatcca ccaaccaccc ataacatctg
     3001 acaatggatt cacccacaag ttacgattca ataatgtacg aaataaaatc atggttgtat
     3061 aaaacaccaa gaaaaataat ctacgaaatt ttgcggatga gcgaaagcat agtatccata
     3121 cttctaatga ctttttaaat ccttgacctg ccaccccttt agcagtcgca aataaataaa
     3181 aatacattgc taatatcgat aacgacactg aaaaaccgaa cggttgatac aatgccgtca
     3241 atacatttac aacaatggaa gaaatcactt taatcatcat tcttcctcct aaactattat
     3301 taaacctcgg ctactggatt ttattcaaca ctcgtcactc acttcgtaaa agctattgat
     3361 ctaggatggc tgctgctcac acaggagctg caacgattta ttctttagta aatacgccac
     3421 gttcgccttt aactatactg ttctctggta caacaccacg aacactgctc agaggataaa
     3481 cctgtgtatt tcttccaatc acagtaccgg gattcagaac actattgcaa ccaatctcag
     3541 taaaatctcc aaggatagct ccaaattttt tgcgaccagt ttcaaaagaa cattcgcctt
     3601 ttacaactac tggggtttta tcgcctttga cattgctggt tatagaacaa acctgcaccc
     3661 atatgagttt tgtaacccag aatactatct cctacataat tataatgggg cacctgaatg
     3721 ccatcgaaca aaatacaatt ttcaagttcg gatgagttgc ctacgactgc accatcacca
     3781 accaatacat tgcctctaat aaaggccccc ggtcttactt ctgtattttc acctataaca
     3841 cacggatccg taatcgttgc caaaggagaa agcttggcac tcttagcaat ccaaacatcc
     3901 tcggcaatct tgttatacct gtcttattca agagatttac taatctgcaa aacggcattt
     3961 ccgatgtcag caagagcttc ccacggatat tcacaggtgt ccaaaaaacg gattgcctta
     4021 atatgggtta aatcaaataa atcatatatc tttattgtac ccatggcaat acccctcaga
     4081 ctgcatatcc attcttcttg atgactgaaa caacagaatc gacatattgt ttgcagattt
     4141 cctctgttgc agcttcaacc ataacacgta ctacaagttc agtaccagat tcacgtacca
     4201 gaatacgacc agaatttccc aatttatcgg ctactgcctt caccgcattc tgcatatcag
     4261 gatcattctg tgcttcggtt ttgtccttta ctctgacatt aacgagcacc tgcggaaaa
//
LOCUS       DACCKU010000146         4295 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.151, whole genome shotgun sequence.
ACCESSION   DACCKU010000146 DACCKU010000000
VERSION     DACCKU010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.151"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            75..488
                     /locus_tag="IVN13_002781"
     CDS             75..488
                     /locus_tag="IVN13_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013210.1"
                     /translation="MLIGGYFLLNLDSVNSNAQIKDQRVAEHNNKKMSLTKNIYSMSD
                     EELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSELVSYKQNHRQFIDV
                     NTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            502..1191
                     /locus_tag="IVN13_002782"
     CDS             502..1191
                     /locus_tag="IVN13_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013211.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1316..3667
                     /locus_tag="IVN13_002783"
     CDS             1316..3667
                     /locus_tag="IVN13_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP7013212.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3747..4142
                     /locus_tag="IVN13_002784"
     CDS             3747..4142
                     /locus_tag="IVN13_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013213.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
BASE COUNT         1503 a          549 c          864 g         1379 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc tacaatatta attgggggct attttctttt gaatttagat tctgttaatt
      121 caaacgctca aataaaagat caaagagtag ctgaacataa taataaaaaa atgagtttga
      181 ccaaaaatat ctattctatg tcagatgaag aattgtataa agaatttggt gatttatccg
      241 gggaacagtt agaagaaagt attccaggaa ttagtgactc agataaaatt gttgtcttgt
      301 ctgctggtgg gcaattaatg ggagatggca aatccgaatt agttagttat aaacaaaatc
      361 acagacagtt tattgatgtt aatactgatt ccaacgctat ttcgattaag gatctaaaag
      421 agcttttaaa tagtaaaaga aatgaattaa agcagataaa agaaactaat tcagtggaat
      481 tagagtaaag gaagattcat tatgaaaaag atttttggtg tttctgttgg gctacttatg
      541 atgttaacat ttggaaatgc agtatatgct gctgagaaag aagttatttc agatccagag
      601 cagtacgcct cacgatactt tgctgatttt gaagaagaag ataaagtact tgcattgcca
      661 gaaggtggtt ttttaaaagg ggaagctaaa attgtatcta tggataatcc agatataatt
      721 caaggttcat ataattcaga aactgatcct gcttcagttt cagtgacaga agcaaaaaaa
      781 attgttgctg aaaaattcaa aaatgatatt gaaactactc caattatgcc tcgaggagca
      841 tttccacccg atgttggtac tagcggacac cttcttgtac tagcaggagg aggaagctat
      901 aaatcacagc catttagtgg tgcgggatgg cgttttggag gtatgggtgt aaaaccagct
      961 gatggtacag gcaacttttt acgttggaaa tcctacattg acgatggaag agtaggaaat
     1021 gtaaatcaag ccaacgcaac taaatctggt tctattcaag gaatggcatt gtatgctcgt
     1081 gaaggattca aatactttgc accaaacggg cctggttgga caatgaatat aggtatttat
     1141 tacacttata atcctgttgc aggtacgtat tatgttgttc aaaatattta ataaaacagc
     1201 acaaagagat ttctttgtgc tgttttatta tttttagttg ttgacaaaca ctaaaatata
     1261 aaatataatc aacacataaa cgaacgtatg ttcggcaaga aagcagggga tttttatgga
     1321 tgagatggtt ttaagtactc agaaatggct aaacaagaaa tacagtaatg ttacagggtt
     1381 tgataaggtt ccagaaaatg ggcgaacagg gtggcctaca atctatggac ttattgaagg
     1441 actacaagta gaacttggta ttactaatct agtagctaat tttggaccta ccactgaaaa
     1501 aatgtacgat aatcaggtta ctcctaaatg gggcaaaaat ctacctaaaa atattgtatt
     1561 tcttattcaa ggtgcctttt ggtgtaaggg gattaatcca ggtggcttcg atggggtata
     1621 tactccatat ttagatactg ctgtaaaaga gttgcagact gatgcagggt ttaatggaag
     1681 cgctgtaact ggagttttgg atgcgaaatg ggccaaagcg ttatttgata tgagtgcttt
     1741 tgttcttgtt cccggcggag atagtaaaat tagaaaaatg cagcaatggt taaatgtaaa
     1801 ttatttagaa tacacgggaa taatgccttg tgatggtatt tatcaaagaa attcaaatca
     1861 agcattaatt tttgcattac aagctgaact aggatattct cccagtgaag caacagggtc
     1921 ttatggtaat gggacaacta gtaaaactta tcctgtgtct gaaggtaact cgggaaacta
     1981 tgtaagaata attcaatatg gattatatgt taatggttat tttgaagacg gtacattcga
     2041 tggtattttc actaaatata tgggtttaga agtactggca ttccgaaaat ttatggtatt
     2101 gcctgaatat actgaaatag cagatgttgt tgtaataaaa ggactattgt ctagcgctgg
     2161 agatattaga agatctgcag atggcgcaga tacttctacg caattaacta gatctcaaat
     2221 ccaaacattg gttgataatg atataaaaat agttggtaga tatcttactg gtactgttgg
     2281 aattggaaaa gatgaacgaa ataagtatct aactacagag gaattgaata atattttttc
     2341 taaaaattta tctgtgtttc ctatctatca agatggaggt gcagcattag cttattttac
     2401 ttatgatcgt ggcttatcag atggtaagaa ggctatagat gcagcaaaaa atctaaatat
     2461 accattaaca acagttattt attttgctgt tgatctagac atgttagggg aagaaattct
     2521 tgcatatgct ggagaatatt ttaagggggt atctgctgta atgtcatata gcgggtatca
     2581 aacgggtgtt tacggaacaa gaaatgtttg ttctcaaata atcaataacg ggtatgcttc
     2641 gtttgcattt gtttcagata tgtcgactgg ttatagtggg aatttaggtt ttcctatgcc
     2701 tagacaatgg tcttttgatc aatttgttga atttaccata ggaagtggca acggagctgt
     2761 tggtatcgat ttaatcgcta catcgggtag agatagcgga tttaatgagt tatcaaatga
     2821 tactaataat gataattaca ttgccaaata caatcagaag gttataaatc agatggtacg
     2881 tgcgtatcaa tatctatcac aagctggtct tgataatcct tggaaccctc atttgacatt
     2941 ttatagatat gtaaattatt ctggtttgtc atgggacatc atttcttctc ctgtaacaga
     3001 gcatgataga aaaatttatg atgaatatag gaataaatta actaatagcg agggcctgta
     3061 taattatttt atagatccta atactggttc aataataggt ttgcctcact taattgtgac
     3121 gttacaatca cagatgtttc tagccgacac aatcaatgat agtgtttctg actttgcagg
     3181 ttggttagga gatttaatga cttgctgggg tgaagttaag aaattaggaa tacaaatgga
     3241 gcaaggtgta tttgcattag taggtacggc tgcaggtgga acatttagtc tagaagattt
     3301 ataccaagat gttgacgcgg taaatgttta taccttagca aaaagaaatt ctgattcaac
     3361 agctttatcg gctttaaaaa cgtactattg tgataagggt gctattgata gatttaatca
     3421 atttgttaag aacagatttg gttcttcaga taaaatcgct tcagtaacaa atagtttatt
     3481 agaaaatgac gaaacagttg atatcttagg tgtagggata atttcatttt tcattgataa
     3541 aatagttaat aattcacttg ggacagattt tacttatctt gattttattg gagatgaggg
     3601 gaaagttgca aatggatttt ctgaaaaaat tattcaattt caagaaaata gaagtaaact
     3661 taattaagaa tcgtttacta tttaaacagt gtccttttcc tttttaaata tatacgataa
     3721 aataagtata tgaattgtaa aaggacatga tgattatgga agaaatagaa tggaataatc
     3781 tgactcaaaa taataaaaat aattctggat gtggaattat ctttatgttt gttatagttt
     3841 tgggaatagt agtactttta ataactgtca agatagtaga ttactttgcg aatactcctg
     3901 aagaacaaat acagagaaaa cttcctgagt ttgaaagagt accaccttat gatccaagag
     3961 ataattcatt tcaagtaaaa aaattaaaag agtctccaaa tgaaatgtgg tttgaagtta
     4021 cagttgaatc aagcgacttc aaagaaaaaa agaaagaata caattatatt gtaaaggcaa
     4081 aagtatcaaa aagatttatt tttaacccta aattagaaat atatagtgtt gtaatagaat
     4141 aatagtgtta aaaaaattgt aaaagttaaa gaagtcaaat tctgatgtga aaaataaaat
     4201 ataccatgga agacataaaa gaaaaactgg ataactagtt tatttaatgg cagaccgtta
     4261 aggcctgcca tttttatgta ttttagcgga ataaa
//
LOCUS       DACCKU010000147         4238 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.153, whole genome shotgun sequence.
ACCESSION   DACCKU010000147 DACCKU010000000
VERSION     DACCKU010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.153"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(250..1677)
                     /locus_tag="IVN13_002785"
     CDS             complement(250..1677)
                     /locus_tag="IVN13_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa12 family transposase"
                     /protein_id="HAP7013214.1"
                     /translation="MMRKQSIEGRNQFAMLTIDDLVPKDHLVRKIDAAIQFDFIYPIV
                     ESTYSTHGRPSIDPVVLIKLVFIQYLFGIRSMRQTIKDVETNVAYRWFLGYSFEDPIP
                     HFSTFGKNYVRRFRETTLFEDIFSHILEQAVKAGFVTEDNLYIDSTHIKANANKHKFT
                     KEMTYGQAKAYQDELEDEINKERIAVGKRPFTWDTESELSLQKISHSDPESGYYVKGE
                     REKQFAYSAHTSCEDNGFILSTLITPGNVHDSQVAIDLVKQSKRAFPNIKRVVADAGY
                     KTPKFVHFLSHLKLYPILPYTVPHGVKGMFRKKDFVYDAYFDCYLCPNNQPLLFSTIT
                     RDGYRTYKSNPKFCRSCPLLKNCTLSTKHQKIVTRHVWADLMDELEHQRHTALNREIY
                     KKRKQTIERIFADAKEKHGMRWTKYRGLEKVATHTMLTFAAMNLKKLATWLWKAKKPM
                     AFYLKFLQRLTKRSWQLSHSFLSTV"
     gene            complement(1822..2370)
                     /locus_tag="IVN13_002786"
     CDS             complement(1822..2370)
                     /locus_tag="IVN13_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013215.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(2371..3228)
                     /locus_tag="IVN13_002787"
     CDS             complement(2371..3228)
                     /locus_tag="IVN13_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7013216.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(3514..3801)
                     /locus_tag="IVN13_002788"
     CDS             complement(3514..3801)
                     /locus_tag="IVN13_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7013217.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSVIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHEKFYEQLKKLI"
     gene            complement(3791..4120)
                     /locus_tag="IVN13_002789"
     CDS             complement(3791..4120)
                     /locus_tag="IVN13_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7013218.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT         1269 a          810 c          623 g         1536 t
ORIGIN      
        1 cagcgacaat aatgacgtct tttttgaatt gtttgccttt aaaatgattc attactctgt
       61 cctctctgtc ttttttctca attttacact aaaatagatt ttttggaaaa ctttgcaaca
      121 gaaccaccgt tcctatgctc attttttgga ttttttaatc acgtaaccag aaaaataaat
      181 cccctctatt tcacgcaaat caaagaagaa ctaagtaact accgtttaaa ggtagttact
      241 tagttcttct cagactgtag acaaaaagga gtggcttaat tgccaactcc tttttgttag
      301 tctttgtaga aattttaaat aaaaagccat aggctttttt gctttccata gccatgtggc
      361 tagttttttt aaattcatgg cagcaaacgt aagcatcgtg tgcgtggcga ctttttcgag
      421 acctcgatat ttcgtccaac gcataccatg cttttctttt gcatctgcaa atattcgttc
      481 aatggtttgt tttctctttt tataaatttc tctattcaac gccgtatggc gttggtgttc
      541 aagttcatcc ataagatccg cccaaacatg tcgagttact attttttgat gttttgtact
      601 caaagtacag tttttaagta atggacaact cctacagaat tttggatttg atttataggt
      661 tcgataacca tcacgagtga tagttgaaaa taacaacggt tgattattgg gacataaata
      721 gcagtcaaaa taggcatcat aaacaaagtc ttttttacga aacattcctt ttactccatg
      781 aggtacagta tatggaagaa tcgggtagag tttcaagtgt gacagaaaat gaacaaattt
      841 aggcgtctta tatcctgcat cagcgacaac acgctttata tttggaaaag ctcttttcga
      901 ctgtttgacc agatcgattg cgacttggct gtcatgtaca tttcctggtg tgattaatgt
      961 agataagata aaaccattat cttcacagga agtatgcgcg gaataagcaa attgtttttc
     1021 tcgttcacct ttaacatagt aaccactttc tggatctgaa tgacttattt tttgaagact
     1081 caactcactt tctgtgtccc atgtaaaagg acgttttcct acagcaatac gttctttatt
     1141 tatttcatct tctaattcat cttgataagc tttagcttga ccgtaggtca tttcttttgt
     1201 gaacttatgt ttattggcat ttgccttaat atgagtagaa tcaatataaa ggttgtcttc
     1261 cgtaacgaag ccagctttta cagcttgttc tagaatatga gagaaaatgt cttcaaataa
     1321 agttgtttct ctgaatcttc gaacatagtt tttaccaaaa gtagagaaat gcgggatagg
     1381 atcctcgaat gaataaccta aaaaccaacg ataagcaaca tttgtttcaa cgtctttaat
     1441 agtctgtctc atcgaacgga taccaaataa atattggata aatactaatt taattaagac
     1501 cactggatca atactgggac gaccatgggt cgaatacgta gattctacaa tgggataaat
     1561 gaaatcaaac tgaatcgcag catcaatctt tcgaactaag tgatctttag gtacaagatc
     1621 atcaattgtt aacatagcaa attgatttct accttcaatc gattgttttc tcatcattag
     1681 aatcactccc agaatagttt atacaatgag tataaaacaa aaaaactgca aacaacactc
     1741 ccaatttagg agtttgtcta cagtctgaga agaactaagt aactaccgtt taaaggtagt
     1801 tacttagttc ttctttgatt attatctaac tttattattc attccccacc ctttgcgcag
     1861 tgagtcttcg gtatcttgtt ttatctgact ggtagttctt attatttctc cacgatcatc
     1921 taattctgga acaattggat atttttctct gaacaagtct agttctttca ccacgtcatt
     1981 aaacaaatct tgcttatctt cttgcgtaag attctgttta atatactcat ctaaagcttt
     2041 tctaagcaat tcatctcttg tcatattagt ctcatacttt tctactaaaa ctctggaagt
     2101 cgcatttaac aaagtcgaca gttttaaagg atgcttggtg gaataaacct tatcaaactt
     2161 tctgattggt gcgccaactt tttttgttct tcctctactc tgtctttcaa cattggagga
     2221 agtttttatt tcttgaactt ggtgcacttc taattgctta ttttcagatg gtgtaattgc
     2281 ttgctgcttt ttctttgctt ctgcttcttt ttttattttg gcgagtcctt tattgttccc
     2341 aaaaatatca agtcctttga tgtcagccaa ttactccacc tccgcatacc aattctcgtg
     2401 ttcttccaat tctttcaaaa tactgataaa taattcgtgg cttttgcgat cccattgttt
     2461 taagagacct ttctttgtct tctccatcgt aatacctgat ccgtcgtatc gttttaagcg
     2521 cgcatttttg agtacaacat ttttaatcaa atgttcccca taaatctctt gggcttgttt
     2581 atacatttcc tgatctactg cgtcaccgct ttccaacatg aaaggaataa caccagcaac
     2641 atataaattt gtatcaaatt ctttcacaaa aaagtccata tactcaagat attgcatcgc
     2701 atttctcatc gattttactt gggtctgtaa gacgacgatt gcccagtccg caatgtacat
     2761 cgcggattta ctatatacag aaatagaagg tgggacatct aaatagatga cgtcaaactc
     2821 atcaaataat ggttctagct tttctttcat atatttgaat tgggataatg aatcattata
     2881 ttggtctttg atccaatcat catacatttc aaaggacatg tctgctggaa gtatatagag
     2941 gttattgatt actttataga cagcctcttt aggtggaaga ttgtttatca acacgtcagt
     3001 cacagtattt tcgtaaacaa tcggttcacc ttctccgcct ccttcttcgt tagtgaagtt
     3061 catatgcgtt aagttcatca catcagaagt atttccttgc atatccaagt caattatcaa
     3121 ggttcttttt ccttcattaa ctgctgattc aaaccctaac atttgcgcgc acgtactttt
     3181 acctacgcca cctttaaaat taccgattac atatttcttc gctaccacgt tcctacctct
     3241 tttcttttag caatattcat ttgatactat tataacatat ttatttttag ttactaccaa
     3301 aaaaaagata tatatgtact accttataat taataaggaa aaggtagtac ataaataagg
     3361 aaaagctgaa atatattgat ttatagcttt cctcaaagta atgacgttat ttaatttatc
     3421 gagaagcaga gaatactcgc gactttagtc atgtagacga atcagatttt cgataaaaaa
     3481 gaagccgtcc ttcttaagaa cgacaacaaa tcactagatt aattttttca actgttcata
     3541 aaatttttca tgcgttccac ataacaatac tggaataacc ttgttttcta taatctcgta
     3601 agctactcta tatgttgtgc ccgcatattt aaacccacta gaccaaataa ctgataaatc
     3661 cccagttttt tgttcaccct tatatggatc aatcgcaatt tcatcataaa ttaaattcaa
     3721 aaatttttct ttcaatggct tctcttttag cttttttaaa tacttaatga catgttttga
     3781 aggaacgatt tcataagcca atttctgttg ccaactcctc tttagtcatt actttagaat
     3841 tagtaagtgt atcttcagca atatctctaa aggaagaatg cattttgttt tttctattct
     3901 taaattctac aagaatctca tctttatcgt agccttctgc aattatatca gacaaaatat
     3961 cttcgctaaa atcataaaat tctttttgtg gttcaacaaa ttcataaaga ataccattct
     4021 ctaccaactt tgcttcaact tcgactccat taggaacatt aaaatcttta ggtactgtca
     4081 acatgattga attgccttgt tttcttgttt ttgtaatcat ttaaatccct catttctaac
     4141 agtatgtaat tacttagtaa atacattata ccacgagata atgtatttac aagtaattac
     4201 tgtgactcgt aatgcgtcct tgctgaataa atttcaac
//
LOCUS       DACCKU010000148         4232 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.154, whole genome shotgun sequence.
ACCESSION   DACCKU010000148 DACCKU010000000
VERSION     DACCKU010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.154"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IVN13_002790"
     CDS             complement(37..1257)
                     /locus_tag="IVN13_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7013219.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IVN13_002791"
     CDS             complement(1277..1882)
                     /locus_tag="IVN13_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP7013220.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IVN13_002792"
     CDS             complement(1931..2863)
                     /locus_tag="IVN13_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP7013221.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IVN13_002793"
     CDS             complement(3284..4144)
                     /locus_tag="IVN13_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7013222.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1202 a          855 c          694 g         1481 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acccgtaccg catctgttgc cccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgagaatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aattggttaa ga
//
LOCUS       DACCKU010000149         4012 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.155, whole genome shotgun sequence.
ACCESSION   DACCKU010000149 DACCKU010000000
VERSION     DACCKU010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.155"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..363)
                     /locus_tag="IVN13_002794"
     CDS             complement(<1..363)
                     /locus_tag="IVN13_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7013223.1"
                     /translation="MKKWLFGFLGVALIVVCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIKMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDA"
     gene            complement(445..1611)
                     /locus_tag="IVN13_002795"
     CDS             complement(445..1611)
                     /locus_tag="IVN13_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP7013224.1"
                     /translation="MSHIQELVEKLINKMSAIFLLLLVSILYLIWTVGLNPPTILFIY
                     DNAAQLVTFFVGCLFLLNLYKVKRNDLFFIAAGILFYLFFSFFRSYRGDTFYGDLIVP
                     TIIAIILIVKWTKFTVIDRSLYLVVFYVFLLVTVYRVFTEIPVPEGQSIWNPSNKLSD
                     IWINTNTIGSSLMTLALLITGFASSFGKWYMRLLSLPALILAGATIWVCQSKAALYAL
                     VIFIILEILPKKVFRNTHLSFWMYAAVSVLALPISYFAAMSDKVHLFTGREEIWRKFY
                     ETLGEKTGQIFVGMKPFFFHRGNQILGNHNTYHALVNHYGLIGFVLSIILLLSFLWLL
                     VRKRELTNGQLTFLWAFLAIMCQSFMEETLTTISWVPIVFLMLAMATHQYKTTR"
     gene            complement(1617..2306)
                     /locus_tag="IVN13_002796"
     CDS             complement(1617..2306)
                     /locus_tag="IVN13_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP7013225.1"
                     /translation="MNKNNKTLFNPYVGVIIVFLLFSYFAYQGHQKDKIAEKVDGVTK
                     TIHIQEQDKTYQDNLTSLTDISKRQLITNQKQALEKDLKGEIAGKLVFSSTGFILPVF
                     QGANSDTLSLGVASTYYPDAEMGNGNYILAGYNVDTPSVLLSDMIDLSEGEEIDLIDA
                     HYKYQYEVSKRGKIYDYSQVVQSKENNSFLSLPKAGKKPLLTLLAFQANNMKKCDVIQ
                     CELVNVEKINK"
     gene            complement(2433..2627)
                     /locus_tag="IVN13_002797"
     CDS             complement(2433..2627)
                     /locus_tag="IVN13_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013226.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYISDMQITGKEKACLLGSMKNFE
                     QLRRTYVKIRSNCQLLLEVS"
     gene            complement(2737..2976)
                     /locus_tag="IVN13_002798"
     CDS             complement(2737..2976)
                     /locus_tag="IVN13_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013227.1"
                     /translation="MARPVLDAMDMDTLTKGRYVQLLARSYSPKDVFKALSKGTDMEK
                     ERLRKEFDYWRIYNGIKDLKPARPVVKRKKKITQE"
     gene            complement(3143..3484)
                     /locus_tag="IVN13_002799"
                     /pseudo
     CDS             complement(3143..3484)
                     /locus_tag="IVN13_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
BASE COUNT         1240 a          820 c          552 g         1400 t
ORIGIN      
        1 ggcatcttca taaaaataag tagccgctcc aagactcaaa gtgtattgat ttgccccttt
       61 aaaaattggc aatgctaaac caagtgcagg aatctgaatt tgtgcaaata cttgtttatt
      121 gacgcccatt ttgattgctt ctttttgtat ggacaacatt tgccccaaag acatatcatt
      181 attgacgttc gttgcatcct ccttataact ctttgcgtgt gcattgattt gatctacggg
      241 catctttttt tctatatttt gcttaaacac ctctccttca tgtttttggt agctaacata
      301 gccaaacaca ctgcaaacga cgattaaagc gactcctaaa aaaccaaata accatttttt
      361 catccatctg cctcctttca gacaaaacaa aaaacacttt ttcacaacga aaaagtgttt
      421 tctcttatta ctattctatt tcatttatct ggtggtttta tattggtgtg tagccattgc
      481 taacattaag aatacaattg gcacccaact gattgtcgtt aatgtttctt ccatgaacga
      541 ttgacacatg atcgctaaaa aagcccacaa gaatgttagt tgtccatttg tcagctcacg
      601 ttttcttacg agcaaccata agaaactaag caaaagaata atcgatagaa caaaaccaat
      661 caatccatag tgattgacga gtgcgtgata cgtattatga ttacctagaa tctgatttcc
      721 tctatggaaa aagaaaggtt tcatccctac aaatatttgt cctgtttttt cgcctaatgt
      781 ttcatagaat ttgcgccaaa tttcttctcg tcctgtaaat aagtgaactt tgtctgacat
      841 tgcagcaaaa taagaaatcg gtaaagctag aaccgaaact gctgcataca tccaaaagga
      901 caagtgtgta tttcgaaaaa ccttcttagg taaaatttct aagataatga agataacaag
      961 cgcatataat gctgctttcg attggcaaac ccaaatcgtt gccccagcaa gaatcaacgc
     1021 tggtaatgat agcaatcgca tgtaccattt tccaaatgaa ctagcaaatc ctgtgataag
     1081 aagggctaat gtcattaaag aactacctat cgtatttgta ttgatccaaa tatctgataa
     1141 tttgttcgat ggattccaaa tactttgtcc ttctggtact ggtatctcag taaatactcg
     1201 ataaaccgtt acgagtaaaa agacatagaa tactacaaga tacaagctac gatcaattac
     1261 agtaaatttc gtccatttta caatcaaaat aatagcaata attgtcggaa cgatcagatc
     1321 accatagaaa gtatctcctc ggtagcttct aaaaaaagaa aagaaaaggt aaaaaagaat
     1381 gcctgctgca atgaagaata gatcgtttct ctttaccttg tacagattca aaagaaacaa
     1441 acagcctacg aaaaacgtca ctaactgtgc agcattatca taaataaata atatcgtagg
     1501 aggatttaac cccaccgtcc atattaaata tagaatggat accaacagta acagaaaaat
     1561 ggcactcatt ttattgatca atttttctac aagttcttga atgtgactca cttcccttat
     1621 ttattgattt tttctacatt gactaattca cactggatca catcgcactt tttcatatta
     1681 tttgcttgaa aggctaacag tgtgagtagt ggttttttac ctgcttttgg caaggataaa
     1741 aaggaattgt tttccttaga ttgaactacc tggctgtaat cataaatttt tcctcttttg
     1801 gagacttcat actgatactt gtaatgagca tcgattagat caatttcttc tccttcagat
     1861 aaatcaatca tatcggataa taaaacgctt ggtgtatcta cgttataacc agcaagaata
     1921 taatttccat ttcccatttc agcatctgga taataagtag aagctactcc taaactaagc
     1981 gtatcgctat tcgctccttg aaaaacaggg agaataaatc ctgtagaaga aaaaacaagt
     2041 tttccagcaa tttcaccttt gagatccttt tctaatgcct gtttttgatt agtaataagt
     2101 tgtctcttag aaatatccgt taaactggtt aaattatctt gataggtttt atcctgttcc
     2161 tgaatatgga ttgtttttgt tacaccatct actttttcag cgattttgtc cttttgatga
     2221 ccttgataag caaaataact aaaaaggaga aaaacaatga ttacgcccac gtaaggatta
     2281 aataaagttt tattgttttt attcatctat ctaccttcta tatttgaatt aatttaccta
     2341 catctaacca agataaaccg cgttaaattt tactattctt tttattaaat aacaatgcca
     2401 catcataatt gatcattttc atatcataat cgtcaagaaa cttctaataa tagttggcaa
     2461 ttagatctta ttttcacata agttcgtctc aactgttcaa aatttttcat tgatcccaat
     2521 agacaggctt tctcctttcc tgttatctgc atgtcactga tatattgcat gacagataac
     2581 cttttaaggt gtttttgttt catcgttttt agtaactttt cttccatttc tttcgacact
     2641 tttttctatt ttcgatttct ttacaattca tgagttttcc taccttcctt gttttgataa
     2701 aaataaaaaa gcctatgatc acatacgctc tacacttcac tcttgggtta tctttttctt
     2761 tcttttaact actggccttg ctggtttcaa atccttgatt ccattataga ttctccaata
     2821 atcaaactct tttcgtaatc tttctttttc catatctgtt cctttggata aggctttaaa
     2881 tacatcttta ggtgaataac tacgagctaa cagctgtaca taccttcctt ttgtcaaagt
     2941 atccatatcc atcgcatcta aaactggtcg cgccatctaa tttgcctcct aaataaattc
     3001 tttactcgat gtctaatgat aatactagct ttttattatt cgtcgtaatt tctgcacatt
     3061 cttcttcatt attttctgcg attaacgcat aatagcatgg tgattttact tcgaaaaatt
     3121 tcatgatcta ttcctccaat ttttattctt ctttatccgc tacaaagtca atgatttcaa
     3181 cactgacaat ataatcactc aaatatgacc aggtatcttc tacagacaat tcgtctcctt
     3241 tgtcgttcgt aagcgtcatt ttaaaccact catcgccttc atcatcttca ggtagcaagc
     3301 cgaatccaat tggattatca gtcaatgttt aatcgtataa atcatcctcg tctaatgcta
     3361 aaacatctaa tcctttacaa tttccgcctc ttctaaacgt cacaaatcct ttgtaatccc
     3421 aacgttggaa agaaacctta atagtatgtg tgcccaatcg tatattcgga tcataatttt
     3481 tcatttgtca ttcctccatt acgtaaattt cattttctct atgttttata tcaatttttt
     3541 cactttattc aggacttgtt catattttat cgtttttcgt ttacttaaaa atacataaaa
     3601 aatttcgact cgtttatggc gtccgaaacg agaaacccta gtagacagcg gtaaatgctc
     3661 gattgatcca ataatcaacg tcctcgcaag tatctacatg tactgcagca cacgacacaa
     3721 tttcttgtgc gtcctcccca ttacgagaag acctactcct tattccatga gtttgcatta
     3781 aagtctagat cccttagtag ctgctcatga ttaatctaga cgctattcta acttgtctaa
     3841 cttctaaatt ccctcttgtt tactctgcgc ttattattta cacaaattca tttcaccact
     3901 atccaaacaa gcgcatttac tcgattgacc aattttttac gttaccaatc gttaaaacct
     3961 accccgtcac tccccgaagg gaattgttga tagatcaagt ctactcctta tt
//
LOCUS       DACCKU010000150         3981 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.156, whole genome shotgun sequence.
ACCESSION   DACCKU010000150 DACCKU010000000
VERSION     DACCKU010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.156"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1200
                     /locus_tag="IVN13_002800"
     CDS             <1..1200
                     /locus_tag="IVN13_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7013228.1"
                     /translation="TIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQ
                     AEWFGNIKGQSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLS
                     TNTLLLGMMGAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSD
                     GMINPLEIFATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGT
                     MKAEFKTYLNRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLE
                     LPGASQEKKNRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRG
                     VQQCQMYMAMTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVK
                     SCGLQVV"
     gene            1888..3765
                     /gene="ltrA"
                     /locus_tag="IVN13_002801"
     CDS             1888..3765
                     /gene="ltrA"
                     /locus_tag="IVN13_002801"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP7013229.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELTLSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     A"
BASE COUNT         1455 a          656 c          889 g          981 t
ORIGIN      
        1 acgatccaag aagtggatga acgggcgcag gaactgatcg aaaatctaaa agggatggat
       61 tatcgtgcgg tgacttatct gcataaaacc aaacgagatt ggcaatcgat gtttaccatg
      121 ttcgatgaac aagctgaatg gtttggaaat atcaaagggc aatcacttcc atcgttaaac
      181 attggcggag gattcccgtt ccaccaccaa acgctaaaag atccaggagg cacacaatat
      241 ggggaaacca tgacaggcgg cccatttgtc tggaatgcca cgtataaaaa tagtgtacgt
      301 ttatctacca ataccttgct tctaggaatg atgggagctg gtaaatcgac cgttcttaaa
      361 atgatcgcgg aagcccattt agctgcagga gactttattt ggggctttga aaagggaaaa
      421 gactttattc ctttcttaaa ggaatataac ggtattatgg tgcgtttaga cggatctgac
      481 gggatgatca atcctttaga gatctttgcc acacggacgt atgatgaagc atccagttta
      541 tatgacgatg gttcagtcaa agatttgaag atcaatgaag cagcgtccta tcaaacgcac
      601 ttagacaaag tagtctatca ggtacagtta gtgagtcctc agctaaaagg aacgatgaaa
      661 gcagaattca agacctactt gaatcgtttt tatgaagagt atggaacagt accacgcggt
      721 tttacttcct caaattcgcg ttcaaatact gatacgcaag tcacagggaa agatccagaa
      781 gcttatccga cgttcaaaga atttttagcc tttttaggac aactggaact accaggggct
      841 tcccaagaga aaaagaatcg gaaagaagaa atggagtcta tcgtggaatc cttatgtgag
      901 acttatggca tgatttttga tggtcatagt acgattcgac accttgacca acaacagttg
      961 gtcttttttg acattgactc tattacaggc ctagatcgag gggtacaaca atgtcagatg
     1021 tacatggcca tgacgttgat ttggaatcag gcattgattc aaggacgaag acaacgacgc
     1081 ttaattaaag aaaaacaaat ggaagaacaa gacttcaaac gcttcttggc gatgattgat
     1141 gagtgccata atggtgcgac ctgttctgca gtgaaaagct gtgggttgca agtagtctaa
     1201 aaactattgt aataactctt ataacgagaa gtcaaatgag tgggtaacgc cacaaagggc
     1261 ttccactgat actccgacat gcaacaagta gtttatgcag accaacgttg tgtgaagctc
     1321 ggtgaagtcg gctgaaggat accgtaaggt aaaagaaata gtgaatacca cgaaagttct
     1381 ttagaggtaa agggtgtatc cgataagtcg ggtgtccaaa aaaacttaat atggtgagta
     1441 tgtacaaacg tactgacgaa ctagcgaatg tactgtccga aaggcatagc tcctagaaat
     1501 gggagagaca gtgagcaaaa aatcactcat tgtgctaact tgtggttgag taaaagtcgt
     1561 tgttatgaaa gaccatgctg cgttaaaggc gtaggagaag ttagggggca aaagctagca
     1621 tctaagttaa ggcatgtgga tagatataag ggaacagggg aagccatcaa catggagagt
     1681 gcatactcga tgaagtggta gtaagaaata tgtgaatgac aaataactta ctttagtgat
     1741 ggtgaaagta gagtcacgac tgatgaaggt tctgtaatgg aactggagga acaggctcga
     1801 ttcgtctgta aaagtcatga aacccgttca accatgggat tctcgtatga ctaaaaaggt
     1861 cacgagccaa ttggagcgtg ataaaccatg acaaaagaaa caagtccaaa agataggaag
     1921 ttgcggcatg cagaatatta tggcatgacg gaaatcttcg ataagatata ctccaaaagt
     1981 ttgaagggag ataatttcaa gaatctaatg cagattatca tttctgataa caacattgtc
     2041 ctagcatatc gtaatatcaa gagaaatcac ggtagtgtga caagaggaga agatggagta
     2101 acgattaaag atattgaaaa tctcaaccgt aatgaattcg tagaaattgt taggaagcga
     2161 ttcgcacatt ataaccctcg gaaagtaagg cgtgtagaaa ttcctaaata taatggtaaa
     2221 ttaagaccgt tgggtattcc atcaatatgg gatagaatcg cgcagcaatg catcttacaa
     2281 gtattagaac caatatgtga agcaaaattc aataatcaca gctatggttt ccgcccaaat
     2341 agatcagcag aacacgcaat agcagattgt atggcccgaa tgaatcaaag tcacatggaa
     2401 tatgtggtag atgtagatat caaaggtttc ttcgacgaag tcaatcatac taaactcatg
     2461 agacaaatct ggtctatggg tatacgagac aaacagcttc tcttaatcat taggaaaatg
     2521 ttaagagcac ccgtagtact tccaaatggg aagttgatat acccaacaaa aggaacacca
     2581 caaggcggaa tacttagccc attacttgcg aacatcaatc tcaacgaatt tgactggtgg
     2641 atagcaaatc agtgggagga aaaagatgcc aaagagctaa aacatcattt taggaaaaat
     2701 ggagcttggg ataaaggtaa tatctatcac aagctcagaa agagtacaac tctaaaagaa
     2761 atatacctag tgagatacgc agatgatttt aaaattttct gtagaaatta taatgaagcg
     2821 aaaaaagttt tctatgcaac taaaatgtgg ttacaggagc gtctaaagtt accaatatct
     2881 gaagaaaagt caaaaatcac aaatctttca aaagaagaaa gtgaattttt aggctttacg
     2941 atgaaattag tgaccaaagg taacaaaaag gttgttaata cgcatatagg tacaaaagca
     3001 caaagcatga ttaaagtgaa actaaaaaaa caaatcaaac gaattcaaaa aagtcctaat
     3061 agcaaagata cctttatcta tattggagtc tataacagta tggttattgg gattcataat
     3121 tacttcaaaa ttgcgactca ttgtagtgta gcaatgcgaa aaattgcaag agaactcaga
     3181 ccaagttttg aaatacgact acgagaacaa ggaatctcaa aagaaggaca atacggaaag
     3241 tacgaaggat acaagcctta cctacggtca ggacaaatac gatatataag agggtttcca
     3301 atattaccag tagggtatat tcaacacaga catccgatgt gtaaaaaaag aaatattaat
     3361 aaatacaccg aagaaggtag ggaagccatt cataagaagc aacaaaccgt agaagaatgg
     3421 aagatatcgt ggttgtgtag caatccagtg cttagtaaga gagcaacgat tgaatttgcg
     3481 gacaatcgaa tttcaaaatt catcgctcaa aagggaagat gtgcagtaac aggtgaggag
     3541 cttaccttat ctgaaatgca ttgtcatcat attatccctt atcatgaaag caaaagtgat
     3601 agctatgaaa acctagtgat tgttactgag gaggttcatc gggtaataca cgctacacaa
     3661 agtgaaacga ttgaggaact tctaaaatat ctaaaattaa atccaaaaca aaaagaaaaa
     3721 ctcaatgagt tgagattaaa agttggtaac gaagaaatag cttaataaat atttgtagga
     3781 cgggaaaaca gaatgtcttt tatagacgag ggaacgccgt atgcggtgaa agtcgcacgt
     3841 acggtgtgga ctgggggaaa agacggagat aatatcaaac tcttacctat cagtatcgat
     3901 taatccagat ttcattgaaa cggtaaatta tgtcacaaac ttccaacgag aaatgcgtaa
     3961 agtagaagca atgactgttt t
//
LOCUS       DACCKU010000151         3956 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.157, whole genome shotgun sequence.
ACCESSION   DACCKU010000151 DACCKU010000000
VERSION     DACCKU010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.157"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..156
                     /locus_tag="IVN13_002802"
     CDS             <1..156
                     /locus_tag="IVN13_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP7013230.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..1452
                     /locus_tag="IVN13_002803"
     CDS             175..1452
                     /locus_tag="IVN13_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7013231.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1462..2118
                     /locus_tag="IVN13_002804"
     CDS             1462..2118
                     /locus_tag="IVN13_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7013232.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2118..3494
                     /locus_tag="IVN13_002805"
     CDS             2118..3494
                     /locus_tag="IVN13_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7013233.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3624..>3956
                     /locus_tag="IVN13_002806"
     CDS             3624..>3956
                     /locus_tag="IVN13_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7013234.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1594 a          423 c          693 g         1246 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaatta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caatttatat tcgagttaat attcatatcg
     1201 ataccaagta taatatcttc cttattttta ggaaatgtat tactaaaagt aattgtagat
     1261 ggattcatta actcagagga ttcaatgatt tccggtggaa gtttaataaa taatagtagt
     1321 tttatgtcaa atataacaac acttggacaa agttatttaa tattaataag tattattgtt
     1381 ttatcagttg tatttgcttc ttcattaatg ttaatcaaga agcctaaaga aatcttatca
     1441 aaaataagtt aggagaaaat aatggatata ttagaaataa agaatgtagc atatagttat
     1501 gcaaattcta aagaaaaagt tttgtcagga gtaaatcaaa aatttgaact tgggaagttt
     1561 tatgcgatag taggaaagtc aggaacagga aaatctacac ttctttcctt acttgctgga
     1621 cttgataaac ctcaaacagg aaaaatattg tttaagaatg aagatataca aaataaagga
     1681 tatagtaatc atagaaaaaa taatatatct ttagtatttc aaaactataa tttaatagat
     1741 tatttatcac caattgaaaa tattagatta gtaaataaat cagcagatga aagtatcttg
     1801 tttgaactag gtttagataa aaaacaaata aaaagaaatg ttatgaaatt atctggtgga
     1861 cagcaacaaa gagtagctat tgctagagca ttggtatcag atgctccaat aatactagct
     1921 gatgagccta ctggtaatct agacagtgtt actgctggag aaataattaa tatattaaag
     1981 acattagcta aagatagaaa taaatgtgta atagttgtaa cacatagtaa ggaagtagca
     2041 gattctgcgg atatcatttt agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg
     2101 aatttggagg ttgaataatg ataaaaaatg catttgctta tgtaactaga aaaagtttaa
     2161 aatcattaat tatcatatta gttattttat ctatgtcaac tttaagtctc attagtttat
     2221 ctattaaaga tgctacggac agagcttcaa aagaaacatt tgctaatata acaaatagtt
     2281 tttctatgga gataaataga caagtaaatc caggaacacc tagaggtggg ggaaatgtaa
     2341 aaggtgaaga tattaaaaag atatctcaaa cagatagtat agactcctat gtaaaaagaa
     2401 taaacagtgt tgcagattta gttgattatg atattattga aactcaagag actcttgcga
     2461 atcaatcacc tgaaagagca aagaatttta aaagaacagt tatgttaact ggggttaacg
     2521 actcaacaaa agaaacaaaa tttgtatcag aagcatataa attagtagaa ggaaagcatt
     2581 tagaaaatga agataaaaat aaaatcttaa tgcataaaga tttagctgaa aaaaataacc
     2641 ttaaagttgg agataagata aaaataaaat ctaatttatt tgatgctgat aatgagaaag
     2701 gtgcagatga aacagtagaa gtagaaatta aaggtctatt cgatgggcat aatagcggtg
     2761 tagtaagtgc agcacaagaa ctatacgaaa atacattaat cactgatatt catacagctg
     2821 ctaaagttta tggtaatacc gaagatacag ctgtatacca agatgcaaca ttctttgtaa
     2881 aaggtgataa gaatcttgat agtgtaataa aagatcttgg gaaattagat ataaattgga
     2941 gagaatataa tttgattaaa agttcatcaa attaccctgc attacaacaa tctatatcag
     3001 gtatttactc aatttcaaat aaattatttt ttggctcatt aatatttgca ggagttgtag
     3061 tttcattatt attattctta tggatgaatg ctagaaagaa agaaatagca gtattactat
     3121 cattaggtat atcaaaatta gaaatttttg gtcaattcct aattgagatg gtatttatat
     3181 caatccccgc attcgttggt tcctatttct tagctcaata tacagctgac aagcttggga
     3241 ataatatatt gaataaggtt actggtgata tagctaaaca aatagctaaa caatctgcat
     3301 ctagccaatt aggtggtggg gcagaagctg aaggatttaa caagacatta tcaagtttag
     3361 atatcaatgt attacctaaa ttcatgattt atgtagttat atttatgagt ttagtacttc
     3421 ttgtatcatt gattatttct tcattcaata tattaagaag gaatccaaaa gaattattaa
     3481 ttgacaataa ttaaaatttt cggtgctttt tagcaccaaa aatgagagtg taatataaac
     3541 cgtgtaagta accatcaatc ctaggcgggt ggttgcttca cggtttttat ttttctaaaa
     3601 taagatcaag aggtgattac acgatggcta gaaagaaaag aaatcctgat gctgagaagt
     3661 tagctgaatc cattttgaat acttatcaac ccgaatctgt agaggatatg caagatgttt
     3721 taaaagatgt gtttggtccc ttatttgaaa aaatgcttca aggagaattg aataaccatt
     3781 tagggtatga cgcccattct aaagagccaa agaaacatga caatcgacga aacggttatg
     3841 gcaataagac gcttaaaacg agctttggta aagtaaacat tgatgttccc agagaccggg
     3901 aggcttcttt tgaacctgag ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DACCKU010000152         3923 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.158, whole genome shotgun sequence.
ACCESSION   DACCKU010000152 DACCKU010000000
VERSION     DACCKU010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.158"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(573..1130)
                     /locus_tag="IVN13_002807"
     CDS             complement(573..1130)
                     /locus_tag="IVN13_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013235.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IVN13_002808"
     CDS             complement(1309..1920)
                     /locus_tag="IVN13_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013236.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IVN13_002809"
     CDS             complement(2033..2290)
                     /locus_tag="IVN13_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013237.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IVN13_002810"
     CDS             complement(2887..3165)
                     /locus_tag="IVN13_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013238.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IVN13_002811"
     CDS             complement(3199..3450)
                     /locus_tag="IVN13_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013239.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACCKU010000153         3887 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.159, whole genome shotgun sequence.
ACCESSION   DACCKU010000153 DACCKU010000000
VERSION     DACCKU010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.159"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            3..1085
                     /locus_tag="IVN13_002812"
     CDS             3..1085
                     /locus_tag="IVN13_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7013240.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
     gene            complement(1303..2445)
                     /locus_tag="IVN13_002813"
     CDS             complement(1303..2445)
                     /locus_tag="IVN13_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7013241.1"
                     /translation="MRKIANVYKDRKRGTWFFSATLGQDRFGKRIQVTRRGFKTQGEA
                     RRGLEELKQSFSDSQGIYLQGITFHDFYREYFFPWYKLGTVEKTYMKTDHILQRALDF
                     FGEMQVVKIRPIHIQEFQQFLSKIHVVKKGIEQPLSPNYIKQIFNKLRVVFKRAIVLE
                     IIEENPVDTIGKIKTQRPQVEFWTVDEFKKVYNSVYHGDFYEEFYKRLMRFIFVTGVR
                     SEELLALQWKDFDLINGRCSVNKSIYIRTRQDYEFAETKNTSSIRIISLDRQTVEDLK
                     TWKKTQQSIGKNIELVFSFDGLPPSPRTILSRIKKLAEIAGVKPIHMHGLRHSHVAFL
                     IEHNKNIYAISKRLGHASIKTTLDKYGHLYPETNQALANEITRFDI"
     gene            complement(2450..2635)
                     /locus_tag="IVN13_002814"
     CDS             complement(2450..2635)
                     /locus_tag="IVN13_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7013242.1"
                     /translation="MKTATRKDLINLGFSQYYANKIFKECKELLVEKGYFFYRNSKIK
                     RIPISILEEVLHISWEE"
     gene            complement(2989..3234)
                     /locus_tag="IVN13_002815"
     CDS             complement(2989..3234)
                     /locus_tag="IVN13_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013243.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMISQLEIKLIEAILKFKIN"
     gene            complement(3228..3629)
                     /locus_tag="IVN13_002816"
     CDS             complement(3228..3629)
                     /locus_tag="IVN13_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7013244.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
BASE COUNT         1289 a          695 c          586 g         1317 t
ORIGIN      
        1 atatcaatga tgatggctca agaaaaaaag tggagaaagt tggcggagac acccgaccag
       61 aggccgaagc tgctttacga aaagttttat cagatattga cgaaacagga caatactttt
      121 taggtacgga tactcgagta aaacaatacc ttgatttttg gatggaggaa tacgttaaac
      181 taaatctaaa atacaatacc tatgaaaact accgatttac catcaaaaat catataaacg
      241 gttatttagg aaagaaaaaa cttacggatc tctcccctgc tcttttacaa aatttcatca
      301 atgctgaatt taaaaagggt tactcgaaga aaacaatgac tattactcac tctgtcctta
      361 agaatgcgct gaatatggcg gtttatcctt ggggattaat caagcaaaat cctatgctgt
      421 atgtaaaaat accaaaatat gaagcacgac caacgactaa aaaagatcta aaaatcattt
      481 ctcttgagga ctttgatcat atgctagaaa ttactcctga aggacatcct ttctatattc
      541 ctttgaatat tggattttat acgggaatgc gcgttggcga agtttgtggt ctgacgtggg
      601 atgatgtcga tttttcaaat ggaacaatta ctgtagagaa acaaatggta aagaatgacg
      661 gcgcatgggt atatggtacg ccaaagacaa gcagttccaa tcgaacgatt tttattggac
      721 aaaccttgct agcaattctg aaaaaacata agaaacaaca attagaaaat cgaatgaagt
      781 atggaaagct ctacattgat tcaaatgcag tatgtacgaa ggaagacggt gagctagtta
      841 cgccaagtgt agtgaaatgg aatactagga ggatatcgaa tgcactctcc ctctctttta
      901 acttccattc tctcagacat actcatgcta cacttcttct cgaaaatggc gcaaaaatga
      961 aagaaatctc tgaacgattg ggccacagca gaatttcaat tacgatggat acttactcgc
     1021 atgtaacaga taagatgaga aatgaaacgg tcgatatcat ggaaaatctt agaaagaatt
     1081 cttgattttt gccaccgaaa aaaaccaccg gtggcaaacc ggtggcaaat ccatgtaaac
     1141 atggtttatt ttttacactg ttttcaaaca aacccttgct atttcttcgg ttcgtcctcg
     1201 tccattttcg ataccgacag ctagcttata acccaaggta tatcaacata aaaaaataaa
     1261 aataggctat gacagcagcg tgacagcaaa aaagatggaa agttaaatat caaacctcgt
     1321 aatttcattt gcgagtgctt gatttgtttc tggatataag tgtccatatt tgtccaaagt
     1381 agttttaata gaggcatgac ctagacgctt agatattgca tagatatttt tattatgttc
     1441 aattaaaaag gcaacatgcg agtgtcttaa tccatgcata tgaattggtt ttactcctgc
     1501 aatttcagct agttttttta ttctgcttaa aatagttctt ggagatggcg gtaatccatc
     1561 aaaagaaaaa accaattcta tatttttccc aattgattgt tgagtttttt tccatgtttt
     1621 taaatcttca acggtttgtc tgtctaacga aattatccga atacttgatg tattttttgt
     1681 ttcagcaaat tcataatctt gtcttgtacg gatgtaaatt gacttattta cgctacatct
     1741 gccatttatt aagtcaaagt ctttccattg taacgctaag agctcctcac tccttacacc
     1801 tgtaacaaaa ataaaacgca tcaaacgttt ataaaattct tcataaaaat ccccgtgata
     1861 aacactatta tatactttct taaattcatc aactgtccaa aattctactt gaggacgctg
     1921 agtttttatt ttacctatag tatctacagg attttcttca ataatttcaa gtacaattgc
     1981 ccttttaaaa actactctca acttattgaa tatctgttta atataatttg gtgataatgg
     2041 ttgctcaatg ccctttttta caacatggat tttcgataaa aactgttgaa attcctgtat
     2101 atgaattggt cgaatcttta caacctgcat ttctccaaaa aaatccaatg cacgttgtaa
     2161 aatatgatct gttttcatat atgttttttc tacagttccc aacttatacc aaggaaaaaa
     2221 atactctcga taaaaatcat gaaacgttat tccttgcaga taaattcctt gtgaatcaga
     2281 aaaactttgt tttaactctt cgagtcccct tctagcttct ccttgagttt tgaagcctct
     2341 tctggtcact tgaattcttt ttccaaagcg atcttgtcct aaagttgcag aaaaaaacca
     2401 agtgcctctt tttctgtctt tataaacatt cgcaattttc ctcattttac tattcctccc
     2461 aagaaatatg gagaacttct tctaaaattg aaataggaat tctttttatt tttgaattac
     2521 gataaaaaaa gtatcctttc tcaactaaaa gttctttaca ttccttgaag attttattag
     2581 catagtactg agaaaaacct agattaatca aatcttttcg agtagctgtt ttcattatag
     2641 caccttcctt actttttttc atttatttct atcagctgct tttgtcaaaa gcacatagga
     2701 atttcacctc tccccaagtt tacggcgagc cttacggaag tatcattatc ccaactttaa
     2761 atcatcgctt tttattgact ataaaaatta ttttagaata tttatcgctc cttctttaca
     2821 gcaagaaatc atggcgcaac taaaatatgc tccttaaagt gttaacgtac tgatagaatc
     2881 tatttaattt tcaatgaaca ataggttttc tacctaaaag aatctactaa ttccttaact
     2941 attagaagat tcttttaaac agaaaaagtg gaaaggggat ttttttaatt agttaatttt
     3001 aaattttaag atcgcttcta ttaatttaat ttctagctga cttatcatat attcatctac
     3061 ataggtaacg aattgaccta cctcgttaaa tccatttgtc aaacataact tagccatata
     3121 agaatgataa tgtttgagta tcatcttgat cgaaattgga tctccttcta gagcagataa
     3181 gataatggat agtggaacta ataggtactg ccgcttttta gatttatcta ccataggtca
     3241 taccccattt tcaattgttc aactgccttc tttcgttgat accagatacc acttgtgctc
     3301 atattgagaa ttttaccgat ttcagcatca ttaaaacctg caaaataata aagtaggata
     3361 atctttcttc ttatctccga taactgatca attgctgctg ataacttttg atctgaaatt
     3421 aatatttcca ttccaaataa gaaatagaca tcttgttcat caaaatgagt ttgggaatac
     3481 ctgccttcat ttgaatctgc caatggttcc tcattatcct ttatgttagc gtttcgtttt
     3541 cgaatagact tagcttcgtt tcgtaatact tttttacagt aggcatcaaa ttggtgttgt
     3601 atagatatgt cattgtaatg ttgttgcatg ataatatgct cctttttccc cctttccact
     3661 aatacaacaa tcagaaagcc cctttttttc gaaaaaacta gaaaaaaaac ataaaaatat
     3721 ttatcattct ttaaacagaa aatctttcta taaaaaacaa aaaaaaacga ttaggctagg
     3781 ctaatcgttt tttgaacatt cttaaaaaat ccaaattatt ttttataact gaaattgggt
     3841 attgatgacc atcgtttctg tagatagtaa gcaatcattt taggttt
//
LOCUS       DACCKU010000154         3736 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.160, whole genome shotgun sequence.
ACCESSION   DACCKU010000154 DACCKU010000000
VERSION     DACCKU010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.160"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVN13_002817"
     CDS             complement(89..1174)
                     /gene="xerS"
                     /locus_tag="IVN13_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP7013245.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3250..>3736)
                     /locus_tag="IVN13_002818"
     CDS             complement(3250..>3736)
                     /locus_tag="IVN13_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP7013246.1"
                     /translation="KEINRTYTKYILEFETIPEELKDKRVEEVDRTPAENLAYQVGWT
                     NLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKELEEILNRNIDDI
                     NMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTFRTKIRKWKRLA
                     L"
BASE COUNT         1213 a          605 c          577 g         1341 t
ORIGIN      
        1 taaaaatgat aatgaagctt gcctttaaag ttgggctttt atctaatttt agagacaggc
       61 ttcattatta tactattttt tactacattt acaaagcgtt caaagcatca attgtagctg
      121 cattgtctat atgagtatat aaagttgttg tatttgtact tgtatgtccc aattgttggg
      181 cagttaatac caatgaattt gtctgttgat agagtctagt agccaaagta tgacgtaatt
      241 tatgtggact gacttggact ctgaatcctt ttgaatattt tgctaccatc cgttcgattg
      301 caataccgga tatacgcttg gcttctcctt gataaattgc tagaaataaa gctttttcgt
      361 tttctgttac tttgtatcgt tctttccgaa tagctagata attagctaaa tattccattg
      421 caaatgctgc tatatttact gcgtctctct tatttccttt tctagttact accacattat
      481 tcttttctaa agataggtcc tctaaattaa tatttacaac ctcggagagt cttaatccag
      541 atcctaaaat caatgcattg atcgccaggt ctctttctac atctcgttga taatagatca
      601 aagctcgttt gctcaaagtt tttttatatt cattggcaat ataatctaga taattttgtg
      661 tatcttcgtc tagaaataat tttttctcga ttgcgtttgc acgggcagct aacgtagttt
      721 tatctgtgat agttgctact tgatccatag gattattttc taagtatggt tttcctaaag
      781 gattattgga acttttcgaa agatatttaa atagaacctt taatgctgtg atatttctat
      841 tgatagtaga agtagataga cctaagttat tattatcttt gagcttagtt tttgttgtct
      901 ctttttttac attttccttt gtgcgacgtt tcaaatgaga tttgtacaag tttagatcat
      961 aggcagttaa ctgtcttatc gaggtgattg aagtatcggg aacgatagac tcactcagaa
     1021 gccattcata aaagagctta taatttttta gatattctaa agtagttcga ggtgattgat
     1081 ctttatcctc acgatcttga acaaaatctt ttatataatc gggcatatga agtatgattg
     1141 tatctatttt ttgatatatt tctgtatttt tcatgttatt catcctttcg ataagtaggg
     1201 gagaggagta tctctataat tggactatac caattataga gtgttttgtc aattggtata
     1261 ctctgaaatt tattaattta tttcaaaaaa gcagataaat aggtatagat ttaataattt
     1321 aaaataaatg gtatacatga ataggaaagg aaacagaagg aggcaatctt aattttttaa
     1381 attgtctaaa ttatatagaa agaacaatct ttctatataa ttagacataa gagagactac
     1441 ggttgatttg atattaacaa gcgtatatga atgtatatac aaataaaaaa ggggagggag
     1501 aagttaaaga aaattcagag aagtggggca ctcagtagta ttaacaattt aaaaggaatt
     1561 tcttaaagaa aatgggttgc atcctggaga tacaattttt ttagataaag aaaaaattca
     1621 agcaacaatt caaaaaaatc ttaactgctg agattgatgt agcaatatct caatcatcaa
     1681 caaagtacga tcctgccttt caaaagctta ttgataaatg tttattcatt ataaaaataa
     1741 aagaaaaatt tatgtcagct tttttcatca aagtggtaat gataattcgt ctattaattg
     1801 tattttttag gaaatatggt ataattgttt taacgaaaag gtaattcatg atacaccaaa
     1861 aaccactagc tggcgggcta gtggttttct tttacgttct attcagatta acgggtaggc
     1921 ttactgtctt ttttggcaat aattttgcaa tcaagccatt tacagatgta atgcgcggat
     1981 acttctgcta agattgcaaa aaacatatcg aaaaggtaat tcataataca acctcctccc
     2041 cttgccataa taggccgggg tagcagacag cataaatatt ataccataac tgaaaatttc
     2101 attttagtac atttcaatat attgatgaac cagcacaata aaacatgcca tgactaacag
     2161 aaaaaaactt attaaaccag atataacttg taaacatcca actatataga aaacgtaaat
     2221 tttctatata gttggattaa gataacgact atttatttta tcatattttt actataccct
     2281 atacttttac ttggtatatt tatgaataat cgcaaattta cccctatact actagtttgt
     2341 aagttagatt aataaaaata ctaaagttat ttgttctttt attaaataca cctacaactt
     2401 ataaattgat ggtatttaga taacacgaaa gaataaaaat aacagctgat agtaaagttt
     2461 gttgacgatt gctactcaat cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact
     2521 tatgaaatga aagacctttt acatcttgca gaaatcggta ctgggattaa actaagcaat
     2581 aaaaactaat gttcccctca ttctttgaac ttctactatt tgatttattt attctgataa
     2641 atgacgattt caccttcagg atcttcaaat cccgtataga atttatttaa gatctcttca
     2701 gtatcagtaa taggataggc gataggccac ataccctttg atggttagaa taaactgctc
     2761 aaaatctgta tcgtatgttg gctttagcat attaacaaag gctatctcca aattataaac
     2821 cataagcgtt gaatcgttct taacaatttc aattcttcct aacccaaagt cagaagatat
     2881 aattaccgga cttgccttac ccttcatgtg ggttgttgag attccatatt taaagggaat
     2941 tgtcggtact atcggcggtc tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta
     3001 gtctcctaag tccctttgta gcggatatgg caccataatc tcattttatc gaagtgagtc
     3061 atggtttatc tgttggccaa ccaaaattga tacaatctaa cgatgcaccc tctacggcat
     3121 tctaggggag tgcacttgca ttcacccaat aaaaaccggc agttagcaaa gaacttatgt
     3181 agtcgtatgt tcttactgtt aacatacaag aggtctcctt tccacaattg cttagcagta
     3241 aacgggaatt tacaaagcta atctcttcca ttttcttatc tttgttctaa aagtcccaaa
     3301 tggagcaact gtatttacat gaataaattt atatacttcc catacagccg ttttggttgc
     3361 ctcgtctgcc cattttcttt tgtgtgcagt gaatagatct tcatcactca ttgaatcaat
     3421 catcatgtta atatcatcaa tattacgatt gagaatttcc tctaattctt tcaatggtag
     3481 atgtgcatag gtatttgtaa accacttata taactctcct aactgattcc atttaaattg
     3541 atcagaaggc gttttaacag ataatccatt tctttcatca ttttcccatt ttagaattaa
     3601 attcgtccaa ccaacttgat atgctaaatt ttcagcaggt gttctatcca cttcctcaac
     3661 tcttttatcc ttcaattctt caggaatagt ttcaaattct aaaatatatt tagtataagt
     3721 cctatttatt tctttt
//
LOCUS       DACCKU010000155         3550 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.161, whole genome shotgun sequence.
ACCESSION   DACCKU010000155 DACCKU010000000
VERSION     DACCKU010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.161"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(320..805)
                     /locus_tag="IVN13_002819"
     CDS             complement(320..805)
                     /locus_tag="IVN13_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP7013247.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            1285..1926
                     /locus_tag="IVN13_002820"
     CDS             1285..1926
                     /locus_tag="IVN13_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP7013248.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1945..2580
                     /locus_tag="IVN13_002821"
     CDS             1945..2580
                     /locus_tag="IVN13_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP7013249.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2598..3413
                     /locus_tag="IVN13_002822"
     CDS             2598..3413
                     /locus_tag="IVN13_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7013250.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         1164 a          578 c          751 g         1057 t
ORIGIN      
        1 actctcatct gtgctgggat attcttacaa aaatataaaa aagtttagtt agaatataat
       61 attttttgtt aaatatgact gaaatcaaaa gaataaaatt ccgatttata atcgaaaaaa
      121 gaagcatttt tatatatgct tcttttttcg tagaatcaat aaatatggat ttggctaact
      181 atggaagaat actcgtatta gttttcagcg ttcatactag ttgttacatt gtttgattgg
      241 ccaaaagaat ttctgatata ttttgtgcta aataaagaaa aagacgcttg aattgggaaa
      301 taagaaaact actctatcat tacaaatttt cataaactgg cagatgttct aagtaaacaa
      361 gtatcttttc aaagtcggaa aaatccataa tcaacgtagt ggtattgata tttggatgaa
      421 agcctacggt atcttgacga tcaatagcag catcgatcac gacttggatt tttttctctt
      481 tgtcgtgcat caatgctaat ggagtcactg tacctgctgg aacgcctagg aggtcaggaa
      541 gctgttcttc agaaacaaaa gataagcgct tttcttttaa ttgttcagct agttgtttta
      601 aatcggcgtg cttttcatca ggcaagatga caagatagat ttgttttcct tttcttgatt
      661 tcaataatag attttttact tgcggaccag gtagggaaaa agggacattt ttgactgacg
      721 taatcgcagg atgatcgact ttttgaaaag agatacccag tttttttagt agttcataag
      781 cttcttgttc ggttgcacga ctcatcgcgc atcaccctcc tttttttatt agtgtatcat
      841 aaaaaagagt tgacaaatga aaaataatca tgtaaattac acgtaaagtt aaaacaaatg
      901 acatcgaaaa gaagagtagc cgttaggtgt ttttagagag tctctggttg gtggaaagag
      961 gcagcagtta accgtgaaac acatcttgga gtcgattttc tgaagcgtat agtaggaaaa
     1021 tccggggagc ccgttacagc tgcggtcgtt gctagatgac cagataaggc tggcttttcc
     1081 gtgaggagtc ggtaaaaaaa ggtggaacca cgtgattaac gtcctttgat cttcggatca
     1141 aaggactttt tgttttcatc tgaacggcaa cgactgtgtg aggttttttc tgcaaggaaa
     1201 aaataaacag aggtggaacc acgtataata cgtcctcttg gcatttgcca agaggatttt
     1261 tttattttaa aggaggaaaa gaagatggag tatttgattg aagttatgcc gcaattatta
     1321 gtaggagcac tcacaacatt gaaattattt ttatttacct tgattggctc attaccatta
     1381 gggattgttt tagcattagg attgtctttg cattttaagc cgctacgcta cttgcttcaa
     1441 acatatgtat gggttatgcg gggaacaccg ctgttgttgc aactgatttt tattttttat
     1501 gggttgccga cgattgggat cgtttttgat cgtttcgaag cagcactcat tgcctttatc
     1561 ttaaactatg cagcttattt tgcagagatc tttcgtggag ggatacaatc gattcctcaa
     1621 ggtcaatatg aagcagcgca agttttgcaa ttaagtccct ttcaaacggt gacgaaaatc
     1681 gtcatcccac aagtgacaaa gattgtttta ccttctgtag ggaatgaagt tatcaacttg
     1741 gtcaaagatt cttccttgat ctatatatta ggtttaggtg acgtaatgcg tgctgggaaa
     1801 atcgcgatgg aacgcgacgc gactttactg cctttgctag gcgtggcaat catctatctt
     1861 acattgactg gtattctaac aatcgtaatg aaacaagttg aaggaaaatt aaattattac
     1921 cgctaactga taggaggaag aaatatgatt gaattgaaga aagtgaacaa agcatttggt
     1981 gaaaaaaaaa taatcaaaga tttgaatcta atcattccag atgggaaaac attagctatc
     2041 gtaggtcctt ccggtggcgg taaaaccacg cttttacgta tcttggctgg cttagaaagt
     2101 tctgacagcg gagaatttta tttagacggt gtaccgttca acccattcga gcttaaaatg
     2161 aaagaacaag ttgtaggagt cgtattccaa gacttccagc tatttccaca tctgaatatt
     2221 tttgaaaaca ttaccttagc accaaaaatg gtattgaaac agacaaaaga agaatacacc
     2281 aaaaaagcag aagaattagc ggcttcatta gggattagtg atttattgaa tatctatcct
     2341 tatcagcttt ctggcggaca aaaacagcgg gtagcgattg ccagagcatt ggctatgaat
     2401 ccacgggtat tggcttatga tgaaccaaca agtgctttag atcctgaatt acgccagcaa
     2461 gtcgagcgtt tgatcctatc attgaaagaa gatggggtaa cccaaattat tgtcacccat
     2521 gatttagtat ttgcagaaaa tgtcggtgat caattactaa aagtttcccc aattcattaa
     2581 gaaaggagaa gatagagatg aaaaaaacca gttttctatt cgctttgatc gcaggtcttc
     2641 ttctttttgc aggctgttcg aacaaaaaaa cttcagcaga taattgggcg actatcaacg
     2701 aagagaaaaa aatcgttata ggtttagacg atacatttgt tcctatggga tttagagata
     2761 aagacgggaa gctcacagga tttgatattg atttagccaa agcagtcttt gaacaatatg
     2821 ggattacggt agactttcag cccattgact ggtcaatgaa ggaatttgaa ctgaacaatg
     2881 gaacgatcga tttgatatgg aacggctatt ccaaaacgcc ggcaagagaa aagaaagtgc
     2941 aatttacaaa accttatatg gaaaacgatc aagtgctgat cacacctaaa gaatcaggaa
     3001 tcacaaactt tggacaaatg aataataaga gattaggtgc gcaaaatggg tcatctggtt
     3061 atgatgtatt tacgaaacaa ccagaagtat taaaagagat agtcaaaaac caagatgctg
     3121 ttttgtatga tagttttaat gaagcattga tcgatctgaa aagcagacgg gtcgatggtc
     3181 tgctgatgga taaagtatac gcagactatt atttgaaaca aagaaacgaa ctagaagact
     3241 tcaacataac aaaaggttct tatgagagtg aggattttgc agttggtgca cgcaagtcag
     3301 atagtacatt agtgaaaaaa atcaatgaag ccctagatca actagagaaa acaggtgaat
     3361 tccaaaaaat atccgataaa tggtttggcg aagatgttac gccgagaaat taacaaatag
     3421 gggactgtga caaaagacgt gtcacagtcc ctttttccga ataaacagtg ttctcctgcc
     3481 aactccttgt gactcggagc tgaatggcag gctcacaacc tcttggtaaa cggtgttcaa
     3541 aagccagttt
//
LOCUS       DACCKU010000156         3399 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.162, whole genome shotgun sequence.
ACCESSION   DACCKU010000156 DACCKU010000000
VERSION     DACCKU010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.162"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..277
                     /locus_tag="IVN13_002823"
     CDS             <1..277
                     /locus_tag="IVN13_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAP7013251.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="IVN13_002824"
     CDS             420..641
                     /locus_tag="IVN13_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7013252.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="IVN13_002825"
     CDS             657..1499
                     /locus_tag="IVN13_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013253.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="IVN13_002826"
     CDS             1562..3364
                     /locus_tag="IVN13_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAP7013254.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
BASE COUNT         1230 a          468 c          702 g          999 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcctatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaata
//
LOCUS       DACCKU010000157         3376 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.163, whole genome shotgun sequence.
ACCESSION   DACCKU010000157 DACCKU010000000
VERSION     DACCKU010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.163"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            254..580
                     /locus_tag="IVN13_002827"
     CDS             254..580
                     /locus_tag="IVN13_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013255.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(697..1041)
                     /locus_tag="IVN13_002828"
     CDS             complement(697..1041)
                     /locus_tag="IVN13_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013256.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(1117..2364)
                     /locus_tag="IVN13_002829"
     CDS             complement(1117..2364)
                     /locus_tag="IVN13_002829"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP7013257.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IVN13_002830"
     CDS             complement(2361..3167)
                     /gene="proB"
                     /locus_tag="IVN13_002830"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP7013258.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
BASE COUNT          987 a          666 c          671 g         1052 t
ORIGIN      
        1 ccgtttacca agaggttgtg agactgtcgt ttaactctga atcacaagga acaatttcaa
       61 aaaacagttt ctcatgtttg ttgaatattg tgacttatga ttgaggagtt ggcagaagaa
      121 caccatttat tcggaaaaag gggatgtgac acgatttttg tcacattccc tttttttctc
      181 aaaataatga aactggaaaa ttttcagtag gtaagctaaa attcatttaa gaaaaagaat
      241 ggggggataa tagatgctgg aagaatattt tcaggaagtc gggttgacac tcgttgaatt
      301 acccattgaa ttttcagatg atcggttgat ttatcgtggc tatatcaaag aattgccaat
      361 aatcaatgcg gaaggtgaaa ctagaaaagc gatgcagcaa aaaatagctg gtatgtatca
      421 ggtgtatcgg gaacagctac tggctgaatc agcggaagaa gaaaaagaaa aaacgatgaa
      481 cttgtccatg gatgagctgc ttcgttatta tgatggcgaa acatttgacg gattttcgct
      541 tcttaacgaa gaggaaacag atttaccagg cagctggtag gcgaacgccg tttatatgga
      601 aaaagaggct gtgacaaaat ctttgtcaca gcctctaaaa agtccggcag ttttttgccg
      661 atcttcaagg cactctactt atcgtacgga tattttttag cggaaagtcc gattcaagtg
      721 gtttttattc agctgatatt tataagataa gtgaaaaatc ccagcaatca gtagcgtaat
      781 cgtataagcg attgaatgat cgaaaaaaga atagtaattg attgtcgcta ataagcataa
      841 tgcaaccatg taactaatgt gcaagcgtgt aaccagccct ttttgcccga ttttgtcaaa
      901 atactgcgac catttttccc gttgactccc tatgcttcgt tttttgaatt gaaagaaaag
      961 ggataaaata ccgactccca gaaaagcagc gatattcaga gaaataaggt aaataaaaaa
     1021 aatcattaaa atttcaaaca tattgacacc tttctagttg gatagaacct ttacagaaaa
     1081 aaggcaaaag gcgggaataa tgtttcacac gaaacattat tcgcggattt gtccatcgcc
     1141 ataaatgata tattttgtgg aagttaattc atttaacccc attggaccac gggcatgcaa
     1201 cttttgagta gagataccaa tctctgcacc aaaaccaaat accgatccgt ctgtaaaacg
     1261 agtggaggca ttgacataga cagccgccgc gtccacttca tttaagaaac gttgactagc
     1321 cgcataacta tctgtcacga tcgtttctga atgtttagta ttatgccgat tgatatgtgc
     1381 aattgcttca tccaaagaat cgaccacttt gacagcaagg atataatcta gatattcagt
     1441 atcccaatct gtgtcttctg ctaaagcagc cgtctctaga tattctaacg cacgagcatc
     1501 cgcacgtaaa gagaccccgt gttctaccag ttctttttca atagctggca agaaaaaagg
     1561 tgcgatctca gaatggatca gcaatgtttc tgccgcgtta cagacagatg gacgggaagc
     1621 ttttgcattg acgatgatgt tcactgccat agataactgt gcttctttat cgatatatac
     1681 gtgattattt cctgtacctg tttcaatgat aggtaccgtt gcctgttctt tgactcgttg
     1741 gattaatttt gctccgcctc gaggaatcag cacatctaag tattctgtca atcgcatcaa
     1801 ctgtcctgca gtttcatgag aagtatcatc gacaaactgg atggcataag gagattcgcc
     1861 agcactgacc agtacttgct gcaataattg gactaataat tgattcgaga agaatgcttc
     1921 tttgccaccg cgaaggataa cggcattccc tgatttgaat gctagactag ctgcatctgt
     1981 cgtgacattt ggacgagatt cgtaaatgat gccggtcacg cctagtggca cgcgcttttt
     2041 gccgattctc aatccgtcgg tgttcgtcca catctcatct acttttccaa taggatcgtc
     2101 taacgaagca attttttcga tctctgtagc catggcattg attcgctcag gtgtcagctg
     2161 caagcggtct tgcatcgttt cactgatatt gttttcgact gctttttcta agtctttatg
     2221 atttgcttca atgatcgctt gttgattctt tttgatggtt tgtgccattt gaacaagtag
     2281 ctcatttttg cggtttgtag gcataagtgc tagttgttgc gcgctatctt ttgcctgttg
     2341 tcctaattgc atcaagtcag tcatttcaag tcctccttaa ataaagtacc tacttctttt
     2401 cctgcaagaa tatcaaaaat aattgccgga ttttttccat ttgctaaaat cattgcgctg
     2461 tttgcttcaa atatcctaga agcggctttt aatttactct gcataccgcc tgttccaaat
     2521 gtgctaccag caccacctgc ttgtgccaga atcttttcat cgattttatc gatatagcga
     2581 tacatctgag cctcgggatt tgtagtcgga ttatctgaat aaaacccgtc gatatcagaa
     2641 aggacgatca aaaggtctgc cgcgacaagt tttgccatga tggctgacag ctgatcatta
     2701 tcgccaaatt ttgtcagatg atctaattca tccactgcta cagtatcgtt ttcgttcacg
     2761 atagggatcg tgtccataat caaaagctgt tccaatgtat tcctcacatt tttacgactt
     2821 tcaggataat ctaccacgtc tcttgtcacg aggatctgtg ccgtctgttg attatataca
     2881 ctgaatcttt gattatagat attcatcagt tcggcttgac caacagccgc cgcagcttgc
     2941 tgttcaggaa tcaaagtcgg acgttctgac aaatgtaatt tatgcatacc aacgcctaca
     3001 gctcctgatg agactaagat cacttccttt ccccgatttc taagatcaga aaggacaaaa
     3061 gctaactgat cgattgccgc aagattgata tttccattgg gataaatcaa tgtacttgtt
     3121 cctactttga caataattcg ttttgcattt ttaatttctt tacgcatgtg acacctcaat
     3181 caaatatcca ctcattaatt tcgttctatt ctacttgttt tgacgagtaa agtcatgtaa
     3241 aaaatagttt ttttgtacaa cttgcagtcg tttggaaaaa gaacgaatta atggtttact
     3301 ttaagatagc agaatttctt gaagaatttg taaagaaagt cttttacagc ctcggatatg
     3361 ttagaatagg agtagt
//
LOCUS       DACCKU010000158         3369 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.164, whole genome shotgun sequence.
ACCESSION   DACCKU010000158 DACCKU010000000
VERSION     DACCKU010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.164"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(215..2212)
                     /gene="tkt"
                     /locus_tag="IVN13_002831"
     CDS             complement(215..2212)
                     /gene="tkt"
                     /locus_tag="IVN13_002831"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP7013259.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2314..2565)
                     /locus_tag="IVN13_002832"
     CDS             complement(2314..2565)
                     /locus_tag="IVN13_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP7013260.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2712..3335
                     /gene="lexA"
                     /locus_tag="IVN13_002833"
     CDS             2712..3335
                     /gene="lexA"
                     /locus_tag="IVN13_002833"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP7013261.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
BASE COUNT          929 a          732 c          625 g         1083 t
ORIGIN      
        1 tttgagttac gtaaatggaa agttttgtct cgcttaattt gacaaataat attctcaaga
       61 aacacaaact aatccgaaga attctttgtt ttgtcattta aatgtaacat agccgaaaac
      121 gaaaagtaat attgatttat tcttctaaca cataaagaac agctgtctcc aacttgcaga
      181 cagctgttct tatcagtcaa acaattgctt ggatctatga tagttgattg tacgtattca
      241 cgacattttc tactgtaaat ccaaattctt ctaaaatttt agtgccaggg gcagatgcac
      301 cgaaatgatc gattgtaatc gttgcgccct ctgttcctac gtagcgttcc catccaaatg
      361 atgcagcagc ttcgatagcc acacgtttct tcactgattt aggaagtaca ctttctttat
      421 attcagaaga ttgtttttca aagaggtcaa agcttggcat agaaacaaca gaaacatctt
      481 tgcctttttc agccaattct ttttgagctt tcacagccaa atcaacttca gaacctgttg
      541 caatcaaaat accttctggt gtttctcctt gtgatggaga aagaacatac gcgccttttt
      601 tcaccatttc atctgctact tcttttgtag aaggcaatac tggaaggttt tgacgactca
      661 atacaaggat agtaggcgca tctgttgtct ccatcgctac tttccaagca gcacgtgttt
      721 catttccgtc tgccggacga atcacttgaa caccaggcat gcagcgaaca gatgctagtt
      781 gttcaatcgg ttcgtgcgtt ggtccgtctt ctccgacagc aaccgagtca tgtgttaaga
      841 cgtaaacaac aggagtatgt tggattgctg ctaaacgaac tgctggtctt agataatcga
      901 caaagacaaa gaatgttcca ccgtagatac gagttccgcc atgaagttgg ataccattca
      961 tagccgaagc cattgcaaat tcgcggacac cgaaccagat gttgcgtcct tcataatgtt
     1021 caggagtgaa atctttatct gcagccacca ttgtattatt ggaacctgac aagtccgctg
     1081 aaccacccca gaagcttggt atagctttcg ataattcttg gatgacttct ttacttgaaa
     1141 cacggcttgc ttgactgctt ccaacttcat aagttggcaa ttctgcatcc cagttttctg
     1201 gtagtttgcc atcaaaagca tcttcaaatt gttgtgctaa ttcaggataa gcttttttat
     1261 aattggcaaa catttcattc catgcatctt cagctttttg tccttcttct atcatcgttt
     1321 gatggaagcg ggcagctact tcttctggaa cagtgaaatc tggatactcc caaccataaa
     1381 cttctttagc catcttgatg ccatcttcac caagaggagc accatgaaca gcagatgttc
     1441 cttctttagg agcaccatag ccgatgactg tttttacttc gatcaatgtt ggtttgtctg
     1501 tttcagcttt tgcttcttca atcgctttag agattgcttc taggtcattt ccgtctttca
     1561 ctaagatatg ttgccagcca tatgcttcat agcgagcacc aacgttttct gtgaaagctt
     1621 ttgaagttgg accgtctagt gaaatatcgt ttgaatcgta taaaacgatc aatttcccta
     1681 gtttcatatg acctgccatc gaacttgctt cttgagatac cccttccatc aagtctccgt
     1741 caccgcatat tgcatacgta tagtgatcca tcacattaaa gttttctttg ttatacgttg
     1801 ctgcaaggtg cgcttctgcc attgccatac cgacagccat tgcaatccct tgtcctaatg
     1861 gccctgtagt ggcttctaca ccatctgtat gatgtacttc aggatgtcct ggtgttttag
     1921 aatcccattg acggaattgt ttcaaatcat cgattgtcac ttgatagcca gctaaatgaa
     1981 gcaagctata caacattgct gacccatgtc ctgccgaaag aacgaaacgg tcgcggtcag
     2041 cccagttttt tgatgtcgtt ggatttactt tcaggtgttt tgtccataac gcatatgcca
     2101 taggtgctgc gcccatcggc aatccaggat gtcctgagtt tgctttttgg actgcttcaa
     2161 tacttaatgt acgaattgtg ttaacgccaa gttggtcaat tttgtcaaac aaaataaccc
     2221 ctcctattaa tttaggtttt ttttaccttt atttcttaca ctttcattct ataagatgtc
     2281 acacgaaaaa gcaatctttt tcagtatttt ttactaaaag ttgtcatttc ggttgtgtaa
     2341 gccttttttc ttttggattt cttttaattt ttccggggtc acgtcattgc cctcaggatc
     2401 gacgactttc attccttcga tatgatgacg cataccggaa cgaaaagctt ccaaatactc
     2461 tttgcgcaag ttgatttgtt cttgttcttc ggcttttgag agaccatttt ccttcttttt
     2521 acgtgcaagt tcattgatcc gctctatttt ttcaggtgac agcatgctca gccctccttt
     2581 tactctcctt catattatat aaaaactgaa aaaaaaacaa ctagataaac taaataaaat
     2641 acgaacattc gtttgattta gcgcttttta tatggtacta tttatttatc aagtgaaaga
     2701 aggtgtacct tgtggcccga caaactgaat caagacaaat cgaagtctta cgatatatcc
     2761 atgaacaagt aacccaaaaa ggatacccgc cgactgtccg tgaaatcggg gaagctgttc
     2821 agctttcttc tacttctact gttcacggtc atcttgcacg gcttgaaaaa aaagggttta
     2881 tccaaagaga tccgacgaaa cctcgtgcaa tcgagttgac aaaacaaggt ttagaaaaga
     2941 ttgggattcg tccaacaact attccagtac ttggcgtcgt aacagctgga gaacccattt
     3001 tagcagtaga ggatgcttca gacttctttc caatccctcc taatctttct tccgaagaag
     3061 gcagtctctt tatgctgaca attcgtggag atagtatgat caatgctggg atattggatg
     3121 gtgacaacgt gatcgttcgg aagcaagaaa ctgctcaaaa tggcgatatt gtcattgcaa
     3181 tgactgccga ggacgaagcg acttgcaaac gattctataa agaaaaaaac tttttccgtc
     3241 tgcagcctga aaacgacttg ctcgaaccaa tcatcttaga tcaagtatct atattaggac
     3301 gagtagtagg actttatcgt agtcatatct attaattgct actaattaaa agaaaacttt
     3361 ttaaaaaga
//
LOCUS       DACCKU010000159         3270 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.165, whole genome shotgun sequence.
ACCESSION   DACCKU010000159 DACCKU010000000
VERSION     DACCKU010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.165"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..584
                     /locus_tag="IVN13_002834"
     CDS             <1..584
                     /locus_tag="IVN13_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP7013262.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(661..1836)
                     /locus_tag="IVN13_002835"
     CDS             complement(661..1836)
                     /locus_tag="IVN13_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP7013263.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            1918..2298
                     /locus_tag="IVN13_002836"
     CDS             1918..2298
                     /locus_tag="IVN13_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP7013264.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2372..2734
                     /locus_tag="IVN13_002837"
     CDS             2372..2734
                     /locus_tag="IVN13_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013265.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1109 a          535 c          620 g         1006 t
ORIGIN      
        1 tggcattccc acaactagat ttaaaaagtg aaaaaggacc aaaagctgtt atcaaaacaa
       61 accgtggtga cattactgtt caattattcc ctgaactagc accgaagacg gttcaaaatt
      121 tcattgaact ttccaaaaaa ggttattatg atggcgtgat ttttcaccgc gttattcctg
      181 atttcatgat ccaaggcggc gatccaactg gaacaggaat gggtggcgaa agtatttatg
      241 gcgaaagttt tgaagatgaa ttcagtcgtg aactgttcaa ccttcgcgga gctttatcta
      301 tggcaaattc aggtccaaac acaaacggaa gccaattctt tattgtaaat aacacaaatg
      361 tgccagctaa tatgcttgga caactagaag gtgctggatt cccatcagaa atcattgaag
      421 cgtataaagg tggcggaact ccttggctag atttccgaca tacagtcttt ggacatgtac
      481 tagaagggat ggacacagta gatgaaatcg caaatgtaca acgtggtcca caagaccgac
      541 ctgtacatga tgtagtgatt gaaacaattg aaatttcaga atagtttggt atggtacgga
      601 gtaattatga aaaaggacga aacctagtaa ggtctcgtcc ttttattttg aataaatgat
      661 ttaatcgatt gtctctaaag catacttgat tcgattaagt ccttccagta aagtttcttc
      721 tggacaagca atattcaagc gcatatgccc atgaccggaa ggaccaaacg tgatgccagg
      781 atttaatacg acttttcctt tccgaacaag agtgttctcc aattcttggt cgtctgaaat
      841 ataggcggta aaatctaacc acattaagta agttccttca gggttcatta ttctcacttt
      901 cggaagatac tgttcaaaaa actgattaac tgtttgaaca tttttttcta gataaggaat
      961 cagttggctt aaccattctc cacccgtctc ataagccgct tgggttccaa tcagtccaaa
     1021 agtgttgatt tcgtgctgtt ggttcatcac taaatgtttt tcaaaatcag ctttcatctc
     1081 tggatttttg ataaatacca ttgaattttt gattgcagct agattgaaag tcttggttgc
     1141 ggaagtaaaa gcaactagta gatcatctaa attaggatca atcgttagca tggaagtaaa
     1201 tgtatgatcg aataaagtta aatcttgatg gatctcgtca ctaaacagaa tcacttgatg
     1261 ttttaaacac aattcgctta actgtttcaa ttcttctttg ctccaaacac gccctccagg
     1321 attgtgagga ttgcataaaa tcattgcttt tacattgttt tcttcgattt ttttctccat
     1381 gtcagacaag tccatgatga aatgattatt tttttctagc aaagagcttc gaacgatgtg
     1441 acgttgattt gtttctataa tagaggaaaa tggaggataa actgggtcat gaatcaaaat
     1501 agaatcgcct ggttttgtaa aagtttgtac tgctgcagct aaactggtta acacaccact
     1561 tgtaaaaacg atattttctt tcgtcaactc cacagcatgg tgttggcgtt cccaatgaat
     1621 gatagcctga tatagtttat cagacagagt ggcataacca aagattccat gtttgatgta
     1681 ttcactaaac gctttagcta ccccaccggg agacaagaag tccatatctg ctacccacaa
     1741 aggaagtaag tcatccattt gatatgtctc agcaattgcg tcccatttta cactttctgt
     1801 atcttttctt gaaatctgtt tatcaaattc gatcatctta agacctccag tcattttcgt
     1861 cgcttctttt tttgagctat ttacagtata atgataaatg cgaagagagg gaagtagatg
     1921 acttataaaa ttggacaaat aattgaagga aaagtgacag ggatacagcc ttatggcgca
     1981 ttcgtttctc ttgataacga gacgcaagga cttattcatg tttcagaagt acaatccggt
     2041 tatacgaaaa atatccattc attgttaaaa gtcggacagc cagtaacagt tcagatcatc
     2101 gacattgatg aatattccaa aaaaatcagt ttatctttaa gaacgttaga aaaaacaaat
     2161 ccagctgttc catatcgcag aaaaaggtat ttcacgaata aaaatcgtaa gatcggattt
     2221 accactattg cggagcagtt acctatttgg atagaagaag cattatctga actagaacgg
     2281 aaagaaaata agaaataaaa agtgtatttt ttctgaaaat gtgctagaat ttttatgtaa
     2341 gccatagaac aagttgatcg aggtggaaat tttggcaagc aaaagagttg ctggtacaat
     2401 tatgttgcat ttggaggatg gtactaaaaa atttttggtc cattcaattg atgacaagtt
     2461 ggaatttgcg ttagcggaac tttctgaagg aaagactggt ttagcgaata tacttaattt
     2521 cttaaaagaa atagttcata tcgatgttag caagattagt ttgatggaat tgacaaatac
     2581 acatattaaa gaggaaaatg ttccgttgtt tgtctttgaa actgaacaaa agaatcaaag
     2641 agaagaatta ggcgaaggat atatttggga agaaccaggt caaatgcgct ctgttcttgg
     2701 aacttacgaa gtagagggag tgcctttctt ctaaaataca gattaagcga acaaagaagt
     2761 ttgaattttt ttcaaacttc tttttttatt gtttggtcca aaaaatacaa tgaaatgttc
     2821 tgctaaagat agttttttag tgcattttat tttcaaaata tgttgaaaac ggttgtataa
     2881 cgaataataa taattgatcg aaaaatcatt tcacaaagta atgcttaatt tatagttgac
     2941 caaggaagaa atacatgata aatttaacag cgttgattct gaagtggatg ttatatcaca
     3001 tttctaaaaa atttaaaaaa tagttattga catgttacta acgacatgat aagataactg
     3061 agtcgttaaa aaagaaccac ttgcgaagct tgattctatc gaaaaaaact tcaaaaaagt
     3121 tcttgacaaa taacaaacga taagttatga taaatgagtt gctgaaagat ttcgaaaaac
     3181 gaaatcgaaa aaaactttta aaaaagagtt gacaatcgct tgtctctctg atatgatata
     3241 aaagttgcta cggcagccaa aaacgaatcg
//
LOCUS       DACCKU010000160         3070 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.167, whole genome shotgun sequence.
ACCESSION   DACCKU010000160 DACCKU010000000
VERSION     DACCKU010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.167"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1068
                     /locus_tag="IVN13_002838"
     CDS             <1..1068
                     /locus_tag="IVN13_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013266.1"
                     /translation="QQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRKM
                     METLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKGK
                     RRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYDR
                     HAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVNQ
                     MQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            1080..1415
                     /locus_tag="IVN13_002839"
     CDS             1080..1415
                     /locus_tag="IVN13_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013267.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            1562..1756
                     /locus_tag="IVN13_002840"
     CDS             1562..1756
                     /locus_tag="IVN13_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013268.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            1788..>3070
                     /locus_tag="IVN13_002841"
     CDS             1788..>3070
                     /locus_tag="IVN13_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013269.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     TPTDSVKEKETDHFYRVEFNENDPAKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKV
                     QNEIKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKG"
BASE COUNT         1250 a          477 c          632 g          711 t
ORIGIN      
        1 caacaaaaag gctcaatcat ttatcaagat gttatctctc acgacaattc gtatcttgaa
       61 aaaatgggct tgtacgatcc caaaacagat accttagatg aagaaggttt gaaagcagct
      121 ggaagaaaaa tgatggagac attattcgag aaagaacaat tgaatgaaac aggttactgg
      181 atggcaacga ttcaccgtaa cactaagcat atccatattc attttgcagt tgttgaaaaa
      241 gaaaatacgc gtaaaccgta tactgagatt gaaaatggtg tgagttatat cgtaccgaag
      301 gggaagagaa gacaagcaac attagacgca atgaaaacca attatgtaca tgagttagaa
      361 cgtttctctt atgaaaagga tcgaatgatc ggtatggaga aacgaaattt attgacacga
      421 aaaagtgatt tgcgtaacac cttaacaaaa gtagtaaaag aaccagcacg ttacgatcga
      481 cacgccatga acttattaaa tgaagtatat aaaaccctac ctgaaaaacg ttcggaatgg
      541 aattatggag atgaaaatcg aaccaagctt tcaccattta caaggggaaa acttgatgag
      601 ttaaccaaat atgttttaac aaaagaacct ggatatcaag aatatataca gatttcaaaa
      661 gcattgaaag aagaagccaa aagcctttat ggggaaagca aaagagaaac aaagaatgca
      721 gaaaaaaacg ccctctttga tttaaaaaag cgcacaggaa atgcgatttt aacagaactg
      781 aagagacagg atagtcaaat aaaaccatta atagaaagtg tcaatcagat gcaatctatt
      841 tacaatgaac gatcctttcg aacactcaaa cagttgccaa caaatgaata caagtatagt
      901 tatcaagaat tcgtaaagcg taggaaacaa ttccaacaaa gagttgtcac gcagcgaagc
      961 ttgcgtcaat taaatcgaac aattaatgca catgaagaaa aataccacgc aagtaaagca
     1021 tatgaagagc aacagcgaag aatccaaatg gaacaagaaa gacaatagga ggaagagaaa
     1081 tgaaacaata tttagatcaa tggaaagtca tagaaggatc actaaaagaa gaacgaatcg
     1141 aacaacttcc tgattgttta gaaaaagagc atttgtttca gataagcgaa atgttgagaa
     1201 acgaacagtt tgatccaaat caatttttag ttgttgagta cccagctact ggtgtgtatt
     1261 gttgtaatca tgtgaagggt gaaaaatatt ttattattca agaatacgaa ggtaagttag
     1321 cgccttatta tactacttgg gaaataaatg aagagggaat caataatttc ccatgtgaaa
     1381 gcattgaaga gtctatttct ctcacggaat gttaaataaa tagagacgta aaagagtcct
     1441 gaacgtgatt gtccaggact ctttttcaat gaattgtttt ccgccaattc actgagtggt
     1501 gcgattcaat tttattatat caaaagaaag tgagattcca aagtgaaggg aggaagaaga
     1561 aatgactcag acaacgattg gtgttgtggt tgaaaaaagc agggataatc tcgtttttgt
     1621 gacagaaatt caaacaggta gagcttttgt agtcacagac aaagctgcaa aagcgtatca
     1681 aaatggcaat attttaacgt taaatctgac tacgaagatg ttcgttgatg cagtagaaaa
     1741 ttatccattt gtttagacaa acgatatttt acaaggagga aacggacatg gcagaagaaa
     1801 aagaaccact agataatagt cgtttgggaa agtccaaaag aaaattagta cggttgcaaa
     1861 acgaattaaa taatcaattt caaaagctat ttgatcacca aaaattgaca aatgggcaac
     1921 caatgaatga caaacgaaat ggtcgttcat ggtttaacca gcaagaaaaa atagaaaata
     1981 aaatacaaag tttgagagaa gagatcaagc aacaagaaaa acaggtagaa aaacttgaaa
     2041 gacaagaaga gctaaaggaa atgggttata acaagtacgg tggcctggac atgacgatcg
     2101 aaaatattcc aagaatcaaa gaagaaatcg aacgctttga aaaaggtgaa tcgacttttt
     2161 cagctgccac gatccgaaaa taccaacgaa aattggaaac attagaacag ctgaaagaaa
     2221 aatcagagaa aggaaaagaa aatcttcttc ctgaagtaca agcgatcatt gacagcggac
     2281 gagtgacgca atggaaaaag aatccaacta tctattttct gaaaggctat cgaaaagtcg
     2341 ctttagaact caatgaaaac gggggatttg aggaatctaa aaaataccaa gccacgtcgg
     2401 aagaagaaaa ggcagctgtt cgtgaattac ttgaagaagc tacacctact gattctgtga
     2461 aggagaaaga aaccgatcat ttctatcgag tagaattcaa cgaaaatgat ccagcaaaag
     2521 ggttgaaaag ttatggtggg gagatcgtaa cactagaatt gatcgatcaa ctcaaaacac
     2581 tggataacca atacgccgca gaagcaggct attataaatt ctattttgaa gaagtgaaga
     2641 atggagaagt agtcgatcag atacgaatgg atctagggga tggcgtcaat agtaatcacc
     2701 ccatctattt agagttagct agattgtgcg aacaatcacc tgaaaaggaa caagaacaaa
     2761 cagtcaaaaa tgaacctatt tcaaaagtac aaaacgagat taaagagaca aaaacaaaac
     2821 aaaagggtta tcaaaaacga acggcagaag agattaagca agaagtcaaa tcactttccc
     2881 aacaggcgct tgatgcggtg aaaaagcata ctcattcacc tgaagacgtc aaagaacttt
     2941 tgaattttat gtctcgtttt ccagaacgat cctttagaaa tcaattattg atcgaggaac
     3001 aatttccagg cgcaacggct tgtttaggtc gtgccgcgtt gaaaaaagaa ggaatctata
     3061 tcaaaaaagg
//
LOCUS       DACCKU010000161         2992 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.170, whole genome shotgun sequence.
ACCESSION   DACCKU010000161 DACCKU010000000
VERSION     DACCKU010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.170"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(106..1053)
                     /locus_tag="IVN13_002842"
     CDS             complement(106..1053)
                     /locus_tag="IVN13_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP7013270.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            1299..1763
                     /locus_tag="IVN13_002843"
     CDS             1299..1763
                     /locus_tag="IVN13_002843"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP7013271.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(1825..2868)
                     /locus_tag="IVN13_002844"
     CDS             complement(1825..2868)
                     /locus_tag="IVN13_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP7013272.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT          990 a          593 c          571 g          838 t
ORIGIN      
        1 agaagctact tctggaacac catttattcg gaaaaaagga ctgtgacaag acttttgtca
       61 cagtcctttt tcatttaaaa aaatgtttac ttcatttgac gtatattatt ggtgaggctt
      121 caattcattt ggcttcactt tttctgtttc tcttatgata taaacaggac gctgtttcgt
      181 ttctaagaag attttcccaa tatatttccc gatgattcca agacataaca attggattcc
      241 accaatgaac aggaagatcg agaccatgga aggccatcct gatgtaggat caccaaacaa
      301 caatgcccgc aaaataatga acaaaattgc aataccagaa ccgacacaag acaaagcgcc
      361 gataaatgaa gcaatcgtta acggtgcatc cgagaagttc acgataccat caatcgagta
      421 gttgaataat ttccagaagg accaagatgt ttcgccagcc acacgttcgc ggttttcata
      481 agaaatatat tctgttttaa atccgaccca gctaaataat ccttttgaaa atcggttgta
      541 ttctgttaat tctaggacgg catccaccat ttgacgagtc atcaagcgga aatcacgagc
      601 gccatcgacc atttctgtat cgctgatttt attgatcaac tggtagaatt ttttcgcaaa
      661 gaaactgcgt atcgcaggct cgccttcacg agtcgaacgc cgggtgccga cacaatcgat
      721 atcagaagta gtgatccggt tgatcatctc aggaagcagt tcgggcggat cttgcagatc
      781 aacatccatc acggtcacta aatctcctgt agaagcttgc aatccagcgt acagagccgc
      841 ttctttccca aaatttctgg aaaatgagac aaagcggaca taaggataag ctgaggctag
      901 cttcttgatt gctgctaacg taccgtcttt tgatccgtcg tcaataaaca aatattcgaa
      961 ctcatgggca cctttgaatt tttcaacctc atcaaaaaaa agaggaacag ttttttcttc
     1021 attaaaacat gggacaacaa ttgatacttt catcgtttct atcctttcaa tcgtatgttt
     1081 atatatggtt tacattcaaa atttaataaa ccttaaataa cacctttaaa atttgatttt
     1141 acgtaaaaga acaggtattc tttttgtaaa ctaacgtatt tagtttacca tgtttcctcg
     1201 gtttatcaaa cttttaagaa caatttttat tagttggcaa gtagttttgg atagagaaaa
     1261 aggaggaagt tccagtctat tgactaggag caaacaaaat gaaaatacac acacctatcg
     1321 gctttttatg gttagatgca gatgaatccg gattgactgc tgtatctttt cagccaattt
     1381 cagaaagcga atcagcgaat cagcgaattt taacgcgcac acaagaacag tttgaagaat
     1441 attttgcggg aaaaagaaaa gaatttgatg ttcctttatc tataaagaga ggtacgcctt
     1501 ttcagcaaaa tgtctggaaa gctttgtcga ccattcctta tggagaaatc cgaacgtata
     1561 aagaagtagc cgttgctgtt gatagtccga aagctgtgcg agcgattggg caggcaaatc
     1621 gaaccaatcc attgccaatc gttattcctt gtcatcgagt gatcgggcaa aatggaaaac
     1681 tgacaggata tatgggaaac gcaatagatg gggttgaaat caaacgccaa ttgttgttgt
     1741 tagaaggata tctgcaaaat taaaaacagt tgtgattcgg acattgttcc atgcgagaat
     1801 cacaactgtt ttttcatgtt cattctaaac tttgatcagc caaataaata gtaaagccat
     1861 cactgtttgg agaagcccga cagctaatcc ataagataag aagcgtgtac cttctttcat
     1921 gaattttgca aagtctaaac gcagaccaat tgccgctaaa gcagtgatct caaaccagct
     1981 gctgataaaa tgtgccgatt ggtcaataac tacaggtaaa gaaaacaagc tgttgaccaa
     2041 acaaaagatc acaaacccta atacatacca aggaagcggt aattttcgtt ttttctgcgt
     2101 atcagttggc gtttcaaaca gagctggttg tttcttgtgc atgaattttt caaacagata
     2161 gacaacaaca acgagcatca taatacgtag aatcttaaat agcatggcga attcgacaac
     2221 agaattgtca agtaagcttg ccccagcgac tacttgacca acagattgga gtgtaccacc
     2281 caaaagtgca cttttttcaa tgagatttcc accaaacaac tgtaatccaa gaaaagggag
     2341 agtcaacatc atcacagtac ccagtagatt gactagtgtg atgatctgtc ctttttcttc
     2401 ctctttcgca tggatggaag gggcgattga agcaatagca gaagaaccac aaacagcgtt
     2461 tcctccagcc atcatcaaag aagaagtatc gctgaattgc agttttttac caatcaagta
     2521 agcaccgatg atagtgactg acatttgcag taacacaaaa acaattcctt ttacaccaat
     2581 ttgagcaatc gtttgagctg tgacagttgc acctagtaaa aaaacagaga attctaatag
     2641 tttgctttca gcaaattttg tgccgattgc tagaacaggc tgtttaaaaa aagtatttcc
     2701 taataaaata cccaataaaa tagcaatagt cgctccacct aactgtggca gataaatagc
     2761 aagaagcctg ctgaaggcag cgatggcaaa agaaagcagt aaaccgggta agatttcgcg
     2821 gaccgctgaa tcctttacag attttattgg atgtgacaat gatttcattt gatcggttcc
     2881 tttcgtcaat aaatactaaa agcatactcg aacatagtag cactaaacca gtattttgac
     2941 aaaagaacaa gaggtcgtga caaaagtctt gtacgacctc ttgtttcgaa ta
//
LOCUS       DACCKU010000162         2947 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.171, whole genome shotgun sequence.
ACCESSION   DACCKU010000162 DACCKU010000000
VERSION     DACCKU010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.171"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(449..865)
                     /locus_tag="IVN13_002845"
     CDS             complement(449..865)
                     /locus_tag="IVN13_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013273.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKI
                     EKQLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKR"
     gene            complement(867..1148)
                     /locus_tag="IVN13_002846"
     CDS             complement(867..1148)
                     /locus_tag="IVN13_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013274.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIEND
                     FDHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
     gene            1948..2922
                     /locus_tag="IVN13_002847"
     CDS             1948..2922
                     /locus_tag="IVN13_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7013275.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKF
                     QEGKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQ
                     LRRGKATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYK
                     YRELLKQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
BASE COUNT          913 a          438 c          603 g          993 t
ORIGIN      
        1 aaaaaaactt gctgatgaat tgggtgtatc gaaaaatacg gtgcgcaatc aactggtcaa
       61 ttcgggattt gaccttgctc agtataaaga aaaaaatgca gtggtcaatt gaccgaaatt
      121 tatgtctttg ggttttgggg ttttaaaacg aaaaaaaggg ggcgattttc gccccctttg
      181 ctctatgtga aaaatttgtt atacttattt tatccttaaa acataccaat ctttttctta
      241 tttagctgct cgtacgagca gcttttttta cgattatgaa ttcaaatcat gtatttataa
      301 tttttttgtt tttatctttt tttctgttat ttcttctgaa attcgttttt ttcattcaat
      361 tggttactat cttattgtaa atgttgctat taagatggtg atcctaccca aaaaataaac
      421 caggaattta aatctcctgg tttatttttt atctttttag aaaatttgtt gggtcgaaat
      481 ttttagaagt aaatgtttga ttggtttctt tttgacgatt ttttggttga tttcgtttgg
      541 agggattaag ttgtttttca attttttctt tcgtttcttg atacacttta gaatttgtgc
      601 caataagaat acctgtatct tttagatata aatcagcaaa ttctgcaatg atttcggaca
      661 tgatggaaat ttcttttccc atagcattta gtttggcttg gttgctggta gtcgtagtat
      721 tggtatcagg ttcataactc atacataaaa aacgaatggc ttccgagtaa ttgttgatat
      781 ctggatttct ttcgataatg gaatctaaaa cgtccacgtg actgggataa agggtaacgt
      841 gtttttgaac agaagttttt ctcatgttat cttcctccga taagtgtttc aatgagttgg
      901 ttgtttgtac gaacatattc attcagcgtt tcagtatttt tttcttgaat agcgataaat
      961 ttttcgagta ataaatcgaa ttgatctttc ttgtaatgca taagtgcgtg gtcaaaatcg
     1021 ttttcgatta ggacttttaa gtagcgattt cgactccatt ttttgccagt tttatttgtg
     1081 agttcttggc aacgtttgtc tatttttgca ataacctttg aatcgatctt ccgaactaaa
     1141 atgtccatag aaaaactcct ttcgcatttg tgtataattt atgtatatca tacgaatata
     1201 gtatttgtat agctgttttt tgctaaaata ctatacataa gctagccacg atggcgaaaa
     1261 agtgatatca aatttttttc tgagaaacgt gataggcaca gcctatctac ccactttttt
     1321 tagaaagtgg gtacacgttt ccttatgctc tttataaaat ggtgatgtgc gacttttttc
     1381 tgttcgtaaa aataaaaaaa gtttacgaaa atgaaactaa actgaacgat tatgacgggc
     1441 atgtaatcaa gatgaaatga ttttataagc gcgtattttg cgttctaaga cgttttagcg
     1501 ttcatttcgt ttagttatcg gcacaatcgt taaaacacgt aagagaagcg agaatggagc
     1561 ctgtaaggcg actggtagcg aaacggcgtg ttagattgtg caagccgaga actgaatgaa
     1621 atgaagagcg gaagcgtgaa ataatttcgg caggaggagg ctcaaggggt tcggggaatg
     1681 aaattccctg aggtcttttt atttttttaa gaggagtgct ataatcaaag aaaacgagaa
     1741 aaaagagcaa aataaaagcc gaacggtgtt ggaagcaccc ttcggctaat tacggaaact
     1801 actcacctag ttccgtaact caaattcatt atacgaatat ttttaggttt cttcaagcta
     1861 gagatttttg gatatggatt tttagagtta cgtgaactct ggaaattctt tccaaaaatc
     1921 tcttttttct ttgaggagga acaaaatttg agtaaacgtg gcacgatcat aaatgagtgg
     1981 gaaaaactta accgcatatc taacaggcgg ttaaatgcac aaactatgtg caccccctta
     2041 aaaatgcaat ggtcaattga ccgaattacc attgttggta cattaaggga aaatatttat
     2101 tatcacactc cccatgatgt gttgatttta gattttgaac agcttatgcg tctaaacgaa
     2161 ggtaacggat atttaaaagc ggttggaaat aatggctggc aattactaga ccaacatgag
     2221 gaaaatatag cttatattga gattcttaag tttcaagagg gaaaagggcg gattgacttt
     2281 aaccccaata aaattagcca atttctggcg ggctcgatga agaactttat ccatgattta
     2341 tttttagaac cacatttctc acgtgcagat attgcttgtg acattattga cgtgccagat
     2401 gatttcatta cccaatatcg ggtggttgat cctgtttctt tcaaaccaat ttacggtcgg
     2461 tctgggaaac tggaaacagc atattgggga agtcgtgcta gtgaacgaca gattcggatg
     2521 tataacaaaa aattggaaca agaaagaaaa cggaaagttg ttccgaaaga aatcgatact
     2581 tggtggcgac tagaattgca attgcgaagg gggaaagcaa ctgattggca tgcaatggtt
     2641 catgaaagtt tagatagctt tgcaagtcct cactatttgc cagaagatgt gaaagtaact
     2701 gacagaatga tgattactgc tcttataacg gaacatagtt actgggggca aattcataga
     2761 aattcaaaat ataaatatcg tgaattgtta aagcaagaaa gtcaaaatga tgagttgacg
     2821 aatcatttgc gagaaacatt ttcggagtcg gcagatgatt tgaaacgaga attagataca
     2881 tggcttcaag gacttgatgt gacggaagta ggtgctgaat gagtgattcg atagaaaaaa
     2941 tgaccct
//
LOCUS       DACCKU010000163         2944 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.172, whole genome shotgun sequence.
ACCESSION   DACCKU010000163 DACCKU010000000
VERSION     DACCKU010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.172"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            6..977
                     /locus_tag="IVN13_002848"
     CDS             6..977
                     /locus_tag="IVN13_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7013276.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="IVN13_002849"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="IVN13_002849"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7013277.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="IVN13_002850"
     CDS             2004..2612
                     /locus_tag="IVN13_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7013278.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          780 a          624 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctc
//
LOCUS       DACCKU010000164         2890 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.173, whole genome shotgun sequence.
ACCESSION   DACCKU010000164 DACCKU010000000
VERSION     DACCKU010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.173"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..466
                     /locus_tag="IVN13_002851"
     CDS             <1..466
                     /locus_tag="IVN13_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013279.1"
                     /translation="NILKDMANIVNVTVESLETINTNSGGAQWVINRPKANYWKKVYL
                     DSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGPKVHEELDFCFATDPIEKVKEV
                     KILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKKKCSYAYVKAIKAVVR"
     gene            463..774
                     /locus_tag="IVN13_002852"
     CDS             463..774
                     /locus_tag="IVN13_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013280.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            764..1117
                     /locus_tag="IVN13_002853"
     CDS             764..1117
                     /locus_tag="IVN13_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP7013281.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            1107..1508
                     /locus_tag="IVN13_002854"
     CDS             1107..1508
                     /locus_tag="IVN13_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013282.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            1501..1905
                     /locus_tag="IVN13_002855"
     CDS             1501..1905
                     /locus_tag="IVN13_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013283.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            1917..2525
                     /locus_tag="IVN13_002856"
     CDS             1917..2525
                     /locus_tag="IVN13_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP7013284.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            2545..>2890
                     /locus_tag="IVN13_002857"
     CDS             2545..>2890
                     /locus_tag="IVN13_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013285.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKE"
BASE COUNT         1070 a          425 c          577 g          818 t
ORIGIN      
        1 aaatattcta aaagatatgg caaacattgt aaatgttaca gtagaatctt tggaaactat
       61 aaacactaat tcaggaggcg cacagtgggt tattaaccgc cctaaagcga attattggaa
      121 aaaggtttat ctggattcta atcggctata tcgctacctt agagggcaaa aaacaaatat
      181 acaaatctgg acagccgaga tgtgggcaca gctttggaac atgatgtatt tcaatattgg
      241 tcctaaagtt cacgaggaat tagacttttg ttttgctact gatccaatag aaaaagttaa
      301 agaagtaaaa atcttacaca atgctggagt aacaacaaac gatgaagatt tatttttcaa
      361 agggagatac gtgacttcta cgccttttga tgaagattta tcatttgtaa acaagaaaaa
      421 atgctcttac gcatatgtta aagcaattaa ggcggtggtt agatgacgcc tgaacaagtg
      481 actgaagaat tgctaatagc tgtgaaggat aatatttacg ttacctggaa cgaagaagat
      541 gagtcaatta aaaagatgat agctaaaaat gctgtttatc ttcaaagtaa agtgagtaca
      601 acactttctt tttctcctga aagcttagaa tacggattgc taatcgaaag atgtagatac
      661 gactggaatc gtgctttaga tgagtttgaa caaaatttcg ctagtgagtt attaggtttc
      721 attcaacatt atgcgctaca agaatatatt gcaggtgatg tgaatggcga ataatcgtag
      781 actcgaagaa acattcaacg atggttggtt aaagattttg acgcaaacta ccaaaagaaa
      841 tgaactagga aaaaagattg gtgtagaaga tacagaaatc acttctttaa aatttagaaa
      901 tctttccatg agagatagtg atataacagc tatggatgcg atgggatcga aattaactaa
      961 gaaagtaaag actccatttc atccaatcgc caagaaattt aataaagatc aatattttat
     1021 cgtaatcgat agtatgcgtt acaacgttat ctatgccgat tacgataatt tttatatcta
     1081 tttttatctt gaaagtgtgg gtgaatatgg tgattgataa ttctaaagaa aaagaacgtt
     1141 taaataagca aatttctgct atcaaaactt ccttagaaga gcattttggc ctcaaactct
     1201 ttcaagactc cgtaggcgag gatgagctac ctgatgattt taattacttc attctcgaaa
     1261 caggagaaat agaaatgatc actgagccaa aatatagcgt gggtcaaaat ctatatctaa
     1321 ctttctattc agaaaataga gaagatttaa caggagattc actagatatt atttcattga
     1381 ttcaaaatcg ttcgattcgt tttcagagaa tggatcccaa tcatttaaaa ctagagaacc
     1441 aagatcgcta tatcgatcaa ttggtattta cgtttagacg attattgaag agtgattgtc
     1501 atggctaaaa atagttggga gctaaaaata aatggacatg atgaacttct tgtgcggatg
     1561 gaacgctatt caagcgagag cgaacgactg attaacgaag cattgaaatc aaaaggttcg
     1621 gctattgcag tggataggat tacagaaaaa attcctgttt ctgaagcaga tttaagaaga
     1681 gggcaccaac acgcaaaaaa tagtcgtcca cttaagactc aatacattaa tttgggtttc
     1741 atcattagac ctacaagaaa atttgagtat ttaaaatatc ctgatttggg gataggtact
     1801 tctaaaagaa atcagccgga cgaatttatg agaagaggat taggtcttgc acttgatcca
     1861 attacagaac ttctgattcg tcaattcgat aaattaaata aataggggga acaacaatgg
     1921 ctaaaacaaa aactgtagta acaacgttcg ataacgtaag tatcaaacga attgctttta
     1981 attttaagaa cgcaggaaat gcaatcgcaa cgaattgtaa tggacaatta gatggcgaaa
     2041 cagaaatgca aacggtggtt aaaaaatgtg gagcgacaga agtaaaatca aaatctaaac
     2101 caatcaatat gacggtaaca attactgcac atgtaccgat ggaagtttat cgacgtttca
     2161 atgggttgaa acaagatgga cgtattaaac caggcattta ctcttacggt cctgattccg
     2221 taggcgaaga tttctcactt gctgcagaga tcgtggatga cttcgaagaa aatagaaagt
     2281 tagttggtat gttagcatgc acttcgaata caggattaac attctctatt gaaaatggtg
     2341 cggatgaagt agctgcgtta gaactagaaa caaaagttat gcaagatgaa tttggtaaat
     2401 tttatcatga agcaattgtt gcagaacttg aagaagactt aacagatcaa tggatgacga
     2461 atctatctgc tgatgtgatt aaaaaaactt cagttgtgac aactacggcc actcaatcac
     2521 agtaaaaaaa acggaggtag cgaaatgaac gaagattact caaaaattga actaaacgat
     2581 ggaacaattt tgaatttaga acctaaactg aatatcaaga aattattgat gatcaataga
     2641 gattttaaca cagacgagtt tgcaaaaatg actgtgggaa aaggatccat ggatatttct
     2701 gttattcaag gtgcaaaggc tgtgtatatt gcttaccgcc aagcgaacat gactgattat
     2761 atttcattcg atgaatttat cgataaatgg gattttgata tggctactgc cagctatatt
     2821 tatcaattga tgatgttcaa acaagcacgc gatgcttatc aaaaagaatt tgaaaaagca
     2881 aataaggaaa
//
LOCUS       DACCKU010000165         2879 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.174, whole genome shotgun sequence.
ACCESSION   DACCKU010000165 DACCKU010000000
VERSION     DACCKU010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.174"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            782..1822
                     /locus_tag="IVN13_002858"
     CDS             782..1822
                     /locus_tag="IVN13_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP7013286.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            2647..>2879
                     /locus_tag="IVN13_002859"
     CDS             2647..>2879
                     /locus_tag="IVN13_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP7013287.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPT"
BASE COUNT         1077 a          372 c          449 g          981 t
ORIGIN      
        1 aagtatatat atttaaaaat ttaagattaa tttttttatg acttatacaa atttatctga
       61 tattctctta ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt
      121 atttaattgc aatttggtag tattttttta ttatcttatt cctagaacgg tagtacttaa
      181 tataaatata ggtagtatct aatttaagta ttaatggtag tatctaattt aacaggtagt
      241 atgtaactac tttaaagtaa ttattttcag attaacacag tagtatctta ttaaataagt
      301 taattttaat ggtagtacat aattattata tatatctgac attggtagta cttattgtat
      361 tttttagtag tatataatct attgttttct agcctaaagc cttgtcttat aagaataata
      421 taatatatac tactgaaata aggtagtata tatcactagt aatggtagta tgttttatat
      481 gcataaggta gttcttaatt tattttttgt tttgacacta gtagtactta attatcttta
      541 attttaaaac taaattttat tcatacgttc ttctggtagt atttaattat aaataatatt
      601 tatttaaagt agttcttaat gactttaaag gtagtacata attcatttta tttctgattt
      661 taggtagtat ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct
      721 attttcgaaa agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac
      781 aatgactgat tttaattttt ttaaggcgga tagagtttat aacgaattat tttatcaatt
      841 tccaaaagtc tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc
      901 ctatatgctt ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga
      961 aggtaatgtt tattttactt atacgacaaa tgaactatgt agcgtattaa actgccaaaa
     1021 acaaaaagcg atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca
     1081 gatgggattt aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt
     1141 aaaagtctca gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa
     1201 cgttgataaa tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga
     1261 atcccttggg gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga
     1321 gggtatgaaa atcataccag aacttaataa taatatatta gacactaata gacacaatat
     1381 agacactgaa aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat
     1441 gacaaacgac agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt
     1501 ttcttcaagt tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa
     1561 agctgaaata gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa
     1621 tgcagaacaa gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa
     1681 agtcgaaaat atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc
     1741 aatagctgct aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat
     1801 tacgaaagac tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa
     1861 cattaaaatt aaaaattttt tattattcta attatattac gaatattaca aaataatagc
     1921 tattttaata gaatttataa gttaaaatat tatgataaaa atcacaaacg tagcctttta
     1981 cgtcataagt gttaaaagaa ataattaatt tcaaatatag aaaagggaac gtatgtttgc
     2041 atactccttt tttttgagtt ataataatac gcgaagagag ataatttcat tatttaaaca
     2101 agctaaaaat cttgatatta cttcttttca ggcaatacga atattattcg cttacatttt
     2161 tttgtataaa tttaattgtc tgctatgcct gtgatgaatt gattttcgtt ctgcactttt
     2221 ggacgataaa tttggacaat tgaaaataca aaaaaaagaa aggcggtatg aactcggaag
     2281 gctggtaatc ttcgtcagcg ttcatacacg gcattaagat ttggccctta atgcgttaac
     2341 ctttcagtgt tacggtatta cccgcaacgg atttagtata acagatattc aaaagtgata
     2401 ccactttttt tggctgtgtt aaggttaaaa atacgtgata ttacgatgaa tctatgccta
     2461 agcataatgt tataaaaatg tccgtgtggc tctttgccgt atgacgaacg ctgaaattag
     2521 agggaacgca tgtttggatg cgttccctct tttttgtttg tcatgaacga aaatcaattc
     2581 tctatttttg aaaaataaca agaaaaactt gttaaaaata tttaaagaaa ttgggtggtt
     2641 tattttatgt catatacagg aattttgagt ctcaaagata tttgtcacta cggtaaacgg
     2701 tgtaccgcta cagagaaaat cacgaagaaa ctatcaactg gccaaagtaa aaccgttgtg
     2761 cagtgtaaga aatatataat ccagaaagac aaagtctcag aggaaatgat ttattacatt
     2821 gggaaacaaa agcaaataat tttaaaagat cctatcccct taaaagagct atatccaac
//
LOCUS       DACCKU010000166         2857 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.175, whole genome shotgun sequence.
ACCESSION   DACCKU010000166 DACCKU010000000
VERSION     DACCKU010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.175"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            562..843
                     /locus_tag="IVN13_002860"
     CDS             562..843
                     /locus_tag="IVN13_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013288.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLEKFIAIQTKNTQVLNDYVHTNNQLIETLIGGK"
     gene            845..1261
                     /locus_tag="IVN13_002861"
     CDS             845..1261
                     /locus_tag="IVN13_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013289.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKT
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(1422..1688)
                     /locus_tag="IVN13_002862"
     CDS             complement(1422..1688)
                     /locus_tag="IVN13_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013290.1"
                     /translation="MAFRFRCDRGGRKMTEKFPYEYYQNLEKLESAIQLVKLDLLDEN
                     LLKYKNITPERKGKLKKMQQLFDEEDGISDVIFDTWSEFHGEYN"
     gene            complement(1646..2620)
                     /locus_tag="IVN13_002863"
     CDS             complement(1646..2620)
                     /locus_tag="IVN13_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP7013291.1"
                     /translation="MGKKRTIVNEWEKLTAPSNRRLNVGESVKMIKMDWSIDRITIVG
                     TLNENIYYHTQNDVLILDFEQLMRVNEGNGHLKAVGNNGWQLVDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKKFIHDLFLEPHFSRADVACDLIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQEAKRKIVPKEVETWWRLELQ
                     LRRGKATDWYEMVQESLDSFASPHYFPEDVKTLDRVMIKGLLYDHSEWSTISRDLKYR
                     LRKLLKQESQNDELTNHLRETFEESADELKRELDTWLLGLDVTEEEEK"
BASE COUNT          926 a          624 c          397 g          910 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatcattt cacgcttccg ctcttcattt cattcagttc tcggctttca caatctaaca
      121 cgccgtttcg ctaccagtcg ccttacaggc tccattctcg cttctcttac gtgttttacc
      181 gattgtgccg acaactaaac gaaatgaacg ctaaaacgtc ttagaacgca aaatacacgc
      241 ttataaaatc atttcatctt gattatatgc tcatcataat cgttcaattt gacatcattt
      301 ccgtaaactt tttttatttt tataagcaga aaaaagtcgc acatcaacat ttttttaaag
      361 agcataagga aacgtgtacc cactttctaa aaaaagcggg cagataggct gtgcctatca
      421 cgtttctaag aaaaaaattt gatatcactt tttcgccatt gtgactagct tatgtatagt
      481 gtttcagcaa aaaacagcta tacaaatact atattcgtat gatatacata aattatacac
      541 aaatgcgaaa ggagtttttc tatggacatt ttagttcgga agattgattc aaaggttatt
      601 gcaaaaatag acaaacgttg ccaagaactc acaaataaaa ctggcaaaaa atggagtcga
      661 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
      721 aaagaccaat tcgatttatt actcgaaaaa tttatcgcta ttcagacaaa aaacactcaa
      781 gtactaaacg actatgttca tacaaataat caacttattg aaacacttat tggaggaaaa
      841 tagcatgaga aaaacttctg ttcaaaaaca cgttaccctt tatcccagtc acgtggacgt
      901 tttagattcc attatcgaaa gaaatccaga tatcaacaat tactcggaag ccattcgttt
      961 tttatgtatg agttatgaac ctgataccaa cacgactact accagcaatc aagccaaact
     1021 aaatgctatg ggaaaagaaa tttccatcat gtccgaaatc attgcagaat ttgctgattt
     1081 atatctaaaa gatacaggcg ttcttattgg cacaaattct aaagtgtatc aagaagtgaa
     1141 agaaaaaacc gaagctaaac taaatccgtc gaatcgaaat cgaccaaaga atcatcaaaa
     1201 agaaacgagt caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgcta
     1261 aaaaataaaa cgagaatttt tttatttctc gttttatttt ttaggaaagc cggtcttaca
     1321 gcaagatttg caagttaccg gtaacttgcg gaaaagaaga agccgctcac gcgacttctt
     1381 ctttttgcat tttctgaaag ctaccacaat tcctaacttc gctaattata ttctccatga
     1441 aattcgctcc atgtatcaaa aatcacatct gaaattccat cttcttcatc aaataattgc
     1501 tgcatttttt tcaatttacc ctttctctct ggcgtaatat ttttgtattt tagtaaattc
     1561 tcatccaaca aatccagctt gaccaactga atagcactct ccaatttttc tagattttga
     1621 taatactcgt atggaaattt ctcagtcatt tttcttcctc ctctgtcaca tctaaaccta
     1681 aaagccatgt atccaattct ctttttaatt cgtctgcaga ctcctcaaat gtttctcgta
     1741 aatgattcgt caattcatca ttttgacttt cttgcttcag caatttcctt agccgatatt
     1801 tcaagtcacg actaatagtt gaccactcag aatggtcata caacaacccc tttatcatca
     1861 ctctatccaa cgtttttaca tcttcaggaa aataatgcgg actagcaaaa ctatccaaac
     1921 tttcttgaac catctcatac caatcagtcg cttttccacg gcgcaattgt aactccaaac
     1981 gccaccacgt ctcgacttct ttaggtacaa tttttcgctt tgcttcctgt tccaacttct
     2041 tgttgtacat ccgaatttgg cgttcgctgg cacgactacc ccaataagcc gtttctagct
     2101 taccagagcg tccataaatt ggtttgaagg acacagggtc aactacacga tattgtgtaa
     2161 taaactcatc aggcacatca ataagatcgc aggcaacatc cgctcgtgaa aaatggggct
     2221 ctaaaaataa atcatgaata aattttttca tagagcttgc taaaaattga ctaattttat
     2281 tggggttaaa gtcaatccgc cctttaccct cttgccattt caaaatttcg atataggcaa
     2341 tattttcttc gtgttgatct accaattgcc aaccattatt cccaacagct tttaaatgac
     2401 cattaccttc gttcactcgc attaattgct caaaatctaa aatcaacaca tcattttgag
     2461 tgtgataata aatattttca ttcaaagttc cgacaatcgt aattcggtca atcgaccaat
     2521 ccatttttat catttttacg ctctcaccga catttaaccg cctgttagag ggggctgtta
     2581 atttttccca ctcattcaca attgttcttt ttttacccaa attttgttcc tcctcaaaga
     2641 aaaaaagaga tttttggaaa gaatttccag agttcacgta actctaaaaa tccacatcca
     2701 aaaatctcta gcttgaagaa acctaaaaat attcgtataa tgaacttgag ttacggaact
     2761 aggctagtag tttccgtaaa ggtcgagagt gttagcgcac tgctcgacta tttttttgct
     2821 cttttttctc gttttctttg attatagcac tcctctt
//
LOCUS       DACCKU010000167         2815 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.176, whole genome shotgun sequence.
ACCESSION   DACCKU010000167 DACCKU010000000
VERSION     DACCKU010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.176"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            155..640
                     /locus_tag="IVN13_002864"
     CDS             155..640
                     /locus_tag="IVN13_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP7013292.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            633..1316
                     /locus_tag="IVN13_002865"
     CDS             633..1316
                     /locus_tag="IVN13_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP7013293.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            complement(1439..2734)
                     /locus_tag="IVN13_002866"
     CDS             complement(1439..2734)
                     /locus_tag="IVN13_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP7013294.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
BASE COUNT          811 a          506 c          573 g          925 t
ORIGIN      
        1 ttttcttggc tgttcttatt atttgatgca ggacagcttt ttcacgacct ctgtaaacta
       61 taggaatctg ttaaatcaag ggagcagaac ttgcctttta cgctactttt ggtacaataa
      121 tgtatgatta aaaataaact ggagggtttc tatcttgaac ataggtaagc cgagaaaact
      181 aggaaaaaca ataaatgaaa tcgaggaagg cgactcgtta tccttaacag aatctattga
      241 agataaccag cttttgctgt atttgggatt gacgaatgat gcaaatcctt tgtatatcca
      301 acatgacttt gcacaaaaaa cagaatatca acagccaatc gttccatcag tgatgctgat
      361 ggggatcatc acaagtgcaa tttcaaagca tcttcctgga ccaggatcac atgttgtcaa
      421 tttttctgtc aattttattg aaccggtttt ccattatgaa acactgactt ttcagtttga
      481 ggtcataaaa gtagataaga tgaaggatgt ggtgacgatt tctgtagaag cggagaatac
      541 gaacgaagat cgtgtgttgg atgctgtcgt tatggtccaa ccgccacaag ttcctgaatt
      601 ggaaatagca atcgaaagtg aaggtgaatt gaatgaataa tccaagtaca tcaatgtcag
      661 gtatcaatcg tttttatgca aaagtttatg gatttttagg tatgggactt gcccttagtg
      721 cagtcacttc ttacttggct ctttgggtat ttcctttgca attagcaact tttatcaata
      781 attttccact aggctttgtg ggtctgtgga tcgttgaact gattttagta atggttctag
      841 gcgtaaaagc acaaaagaat ccgagcttag caatcggtgg atttattgcc tactctcttt
      901 tgaatggcat aacattagcg gttactcttg catactacga tattggaaca gtaacgcaag
      961 ctttcttgac agctactttg atgttcatgg ggatgtctgt tgtcggagta acaacaaaaa
     1021 gagatcttag cggggttgga agagcaggac aggcagcttt aatcggagtg atcattgcac
     1081 tagtcttgaa catcttcatt ttgagaagta gcgtagttga gctgttcatc tccctactgt
     1141 tagtcgtcat ctttgcaggg attacagctt atgataacca aagcatccgt aatatctatg
     1201 tccagacaaa cggaacagca catagtggaa ttgctgtatt catggcattg aagctgtatt
     1261 tggactttat caacttgttc ttggcgtttc tgagaatctt tggacggaac aactaaaaaa
     1321 gaaaaaaatt catcagttgg ggctataccg aattatggct ctttgtcaat aaggactgat
     1381 gagttgtgca aaatcaaatc tgagtcagaa tgaaccacat tctggctcag attttttgtt
     1441 atcttacatt gattaattga ttgatcaaaa agattctaat tctcatgttc tcaaatgatt
     1501 taaatccgta ggatactcgt ttcattgtct ttatgtgggt attcttcgct tctatttttc
     1561 cattggaata aggatagatc attgcgttgg tgatgccttc ttcataggtc agaaggtttt
     1621 gaagcttttc ccgaaagctg tcatctaacg tttcgggaag ttctgccaat aaggagaaaa
     1681 ataagtcagg gtctttgtct cgaaaggctt caactaattc atgaaaaaag ggatacgcct
     1741 cctttagggg cggagaaaac tcaagcaatc gatcaatcat cattgcttca gtaagaaatg
     1801 ggtattttgg tgctcggaaa cttttccatg ttttgtattc ataatggttg atgtttgcac
     1861 gattttttag caaaaagcgc cagttctttt tcagtttttc tgcctggctt ttctgtccgg
     1921 ctttacgaag ttcattcatt tcacggatac gcaactcatt gaacgcttga ttcatgtgtt
     1981 tgacaatatg aaaccgatca atcacgactt tcgcatttgg cagaacacgt ttggtgagct
     2041 ggaagtaggc ggcgttcatg tctgtcacca agaattctac ttcttctgga ttggtacaac
     2101 ctaagaaata gcttgttaat cgaggtaatt tacgcgtagg caaaacatct attaattttc
     2161 ctgtttcgcc atccgcgcaa ataaagctca tcttatcttc tatggaagca tgcgaacgaa
     2221 attcgtcaac catcaatact ctggggagga tcttcttaga ttgctttggt aaatagcttt
     2281 taaactcttt caatgtacga ataacggtgg tcaaagatac ctgacagctt ttcgcaataa
     2341 aagataaaga tactttttca gtcagtaaag aagcaatttt atatctaaca tgatttgcga
     2401 ttgaatgtct gggttggaca aaataacttt gagccgtcca atgggtgcga cagtttttac
     2461 aggtatagcg ttgctttttt aggcgcataa ccaaaggcat atgattgtat tgttcaaaac
     2521 ggacaatcgt ttcctttttt ccatttttca ctataattgc tttcccgttt ccatctacca
     2581 cagtagaacc acaacttcta caagcacgag gagtaggcga gagaacagca tcgacgacca
     2641 acgtcttttt cttctgaagg gtctcgtaag agacctctgt aatcatcaaa tctttctctg
     2701 ttattctcag catttttttg atagaatcat tcatatagcg tatcgtcctc tcagttgttt
     2761 attttgtggt gatttaatca tactagagaa cgatatgttt tttaatacct aaaat
//
LOCUS       DACCKU010000168         2749 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.177, whole genome shotgun sequence.
ACCESSION   DACCKU010000168 DACCKU010000000
VERSION     DACCKU010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.177"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            6..980
                     /locus_tag="IVN13_002867"
     CDS             6..980
                     /locus_tag="IVN13_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP7013295.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAIIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
     gene            complement(2287..2652)
                     /locus_tag="IVN13_002868"
     CDS             complement(2287..2652)
                     /locus_tag="IVN13_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013296.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          999 a          349 c          449 g          952 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtca ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaattat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgatga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgttggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaagcatttt ttcttgatgt gatgtccttg ccttttttgt
     1081 aatgttttta acttattgcc tttgcaaaat ttatttacct gttttctgga tcaacgatag
     1141 tgtatattat gctatccttg tttggatttt aggacgttta aagaggcact tttttacata
     1201 acttaatcca tagacctttt aagcatgctg tgagataata ataaagaaga gtttaaagcg
     1261 caccccaaac cacttcccca taagtgtatt acgctttaaa ctcttttata tttgaattca
     1321 ctaagaagca taccatatat ttgaatttac taagaagcat accatatatt tgaattcact
     1381 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1441 gtaattaatt agatgtttat tttataggac tgatactata gattacaaat agtatcgtat
     1501 ttcgcaatct taaaattatc ttcttactat gaataagata ataaaatttg tatttgtcct
     1561 ttttaggtaa cttatttaat ctatatacct ttctggatca acgaaagtat actttatata
     1621 gtcataacgc cgatggtaac tatgcactgt aacgataatg ctgctaaaat atctttttac
     1681 gaaaaacatc acaaggaaaa acttaactat ggtattatct aaacataatt ttttcggtat
     1741 ttaatattta attaggagga aatattttgc ttttttatag taattttatc ttaatagtag
     1801 cgatattatt acttctgaat atatggattt ttgataggag tagaaatgca agtataggtt
     1861 ttagaactaa acgaagtctg tcttctaaaa aaattgggta tactcacaaa ctatttttta
     1921 tggaggaatt gttttaatta gtttactttc ttcaacactt tattctttga atattataga
     1981 tgtttccacc tcaaattcaa tatctattat tggaatcatt attgctgcaa ttattacaca
     2041 actgttttta gtgtttggag agaagaaaag aagtaaaaaa tagaaaaact agtgatagtt
     2101 ttggtgatag tgatattaaa aatcagaaaa gatagaaaaa gcaggagata gataaaatgc
     2161 tgatatttca gcatttttta agagtataaa agagaaaaaa agtaataaat gtatacatga
     2221 tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta catatatggt
     2281 ttgtttttat ggatgccgag cttttttata agataaggct accgcccata acgacccaag
     2341 gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg gttttaaagg
     2401 atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag tgacagtaga
     2461 tataatattg gcaatgaata gagatataat acgcctgttc ctttgaaaat aatatccagc
     2521 aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac gaaaatcaag
     2581 cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt ttttccagca
     2641 agtttttatc atctttcatc accacctaac aagtgttatt attttgttag ttttattgta
     2701 aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaa
//
LOCUS       DACCKU010000169         2732 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.178, whole genome shotgun sequence.
ACCESSION   DACCKU010000169 DACCKU010000000
VERSION     DACCKU010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.178"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..335
                     /locus_tag="IVN13_002869"
     CDS             <1..335
                     /locus_tag="IVN13_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP7013297.1"
                     /translation="KLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKK
                     TGGLRYAPALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKRED
                     FNEAQKIR"
     gene            351..1112
                     /locus_tag="IVN13_002870"
     CDS             351..1112
                     /locus_tag="IVN13_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP7013298.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            1243..2577
                     /locus_tag="IVN13_002871"
     CDS             1243..2577
                     /locus_tag="IVN13_002871"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP7013299.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT          926 a          464 c          585 g          757 t
ORIGIN      
        1 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
       61 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
      121 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
      181 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
      241 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
      301 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
      361 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
      421 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
      481 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
      541 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
      601 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
      661 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
      721 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
      781 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
      841 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
      901 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
      961 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
     1021 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
     1081 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
     1141 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
     1201 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
     1261 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
     1321 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
     1381 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
     1441 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
     1501 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
     1561 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
     1621 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
     1681 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
     1741 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
     1801 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
     1861 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
     1921 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
     1981 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
     2041 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
     2101 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
     2161 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
     2221 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
     2281 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
     2341 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
     2401 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
     2461 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
     2521 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
     2581 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaaaaat cttgccaagc
     2641 cccctttttt gcaacttatg ataaacggtg ttcaaaagct tgcatctgcg gtaataagcc
     2701 caatcaaatc aaaaacatga gaagctgttt tt
//
LOCUS       DACCKU010000170         2716 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.179, whole genome shotgun sequence.
ACCESSION   DACCKU010000170 DACCKU010000000
VERSION     DACCKU010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.179"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1015..1305)
                     /locus_tag="IVN13_002872"
     CDS             complement(1015..1305)
                     /locus_tag="IVN13_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013300.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1513..2205)
                     /locus_tag="IVN13_002873"
     CDS             complement(1513..2205)
                     /locus_tag="IVN13_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP7013301.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          802 a          505 c          388 g         1021 t
ORIGIN      
        1 catacttgca aagtaatact acaattccta acataatcgt agaatttaat aatttccata
       61 gcataaagtg tttagaaaag aacattaaga atagttctat aataattctt gaactccatg
      121 tcttataatg ttctatcgaa gcacccaaat agccaagttc atctaatcta cttgcataaa
      181 ctacgtcatc cgctctcata gtcggcatat acatattaaa gagtaaaaag ataccccaaa
      241 tccctattag taacaagatc agttctttgt tattcgttat ttttctatta tgtttgttca
      301 tttttatact ccttcttata tttcctaata gagtataaca tagctttaag cattacttaa
      361 agataactta aaagttatct tttaaatact taattgttaa ataccttatt tgatgcaaaa
      421 gaaatctacg atagtcaaca aaaaaatttt cattatatcc agaaaaattt ttcttagtta
      481 aggacctatg gattgcagta atgcagaatt cgtcgaataa actgccaaag acatacaatc
      541 atattgcttt taaaattgac gaacaagata tagattcgtt tgtctcaaaa atccaaatgt
      601 taggtttaac tgtcgaacca ggacgttctc gtgtgaaagg cgaggcttct tcaatttact
      661 tttacgacta tgataatcac ctcttcgagt tacataccgg tactctccag gaacgtttaa
      721 aatcctacaa ttccactacc tgagactagt aatgactagt ctctttttat ggcctctgat
      781 ccccccattg agcgaatact aatagggctt cgaattgatc tgttcaatct ttaagcaata
      841 tccctcaaat gagtttggaa agtttagtga ttcatcacaa atacttaaat acatgtagtt
      901 atatatagat tgaggttctt gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc
      961 atccatcttt ccaaaacaag cggctaggac atttttgtct cagccgcttg ttttctagtt
     1021 tattctttca tcttgtcgga ccttttttct tgggtataga cttttctcat attctaccac
     1081 aattttgggt ttccctaaca catgatagga tctcacaaca aattgctttc tattattata
     1141 ataacctgta acgactaact tcatcccttc gtctacttca gctaggaagt tcaaactatg
     1201 ggaagcaatc aaacaattga ccccatttaa actgaaacgg atcaacggtc tggcactaac
     1261 ttgcaaagta cggatcttat ctactgttcc atattttttc tccataacct tcacctcaat
     1321 accattatac gaacatacat tccctttgta aagaagaaac tctgttttat ctttttgact
     1381 ttttgtctat tacctttctt aaaatcatat tggcacttcc tgcgaagatg aaggacatgt
     1441 actaaaaaaa agaggcagca gtatagatta ctgttgcttc tttactaatt taggtttact
     1501 tgttaagaaa gattatttac gcaaatgttc ttcaacaatt tgctgagatt tatcagctgt
     1561 atcttccact tcatcttttg ctttttctaa cttttgaata tattgtttca agcttgagtt
     1621 ttcagcagtt gcttcgtcta atttcttttg tgtatctgtt aactgagttt ctacatttgt
     1681 tttttcttgt gtcagtgttg cgatttgttg attcaaacca tttacagtat tttgtaattc
     1741 tgaaagtttt gcttgtgctt cttgtagtct tgctgtccaa tctgcattca gatgattgat
     1801 ttcatcattt ttttggttga tctcatctag tttgacattg atttcatttt gtttgctttg
     1861 tagttgtgca tccttgctga ctaattgact ttctaagttt tgtttttctg ctgccgattg
     1921 atttttttgg ttttctaatt catttacttt tgtttggtat tcttggacat ctgcctttga
     1981 atcttgcaat gtctgttctt tttgtgctag tcgagcggta agatctgtaa tcgcttgctc
     2041 tttatctgtc aatgtatgat caagctgtgt cagattctca ttgattgtat caatatctgt
     2101 ctctccaccc cacaaaataa tttcatttgc gtaccgcgtg cctgtgtagc caattagaca
     2161 tagaaatgca acgacaatcc caccagtaat aaatttcttt ttcatttgca tagctcctta
     2221 atccaatact tttacatgct aaaagtaagt gatctcaaac acaatgtcaa gcgtttttaa
     2281 attacatatg taattttaat ataaatacta tcaaatactc atttaatcct ccttttttat
     2341 ctatttaaaa aatctttttt atagagaaaa atgcaggtta atactttctt aaataaatat
     2401 ttatggtgaa caaaaacaaa aaagcaagtt ttattaaacg taaccctttt catttcatag
     2461 attactgtat ttattacaaa aaacgcggat ttgtcataaa ttgtcatgct tcttttacct
     2521 atgaaatatt ttcacgtgtt aaagtattcc tcgttgaaca caacaaataa aactttttaa
     2581 cgaataaagg agatttttca tgacatcact taaaacttta ctttttggaa caactttggc
     2641 tgccggcgct gcattcttca tgggaacgac tgctcatgcg gacgaagctt ataccgttca
     2701 atcaggtgat actcta
//
LOCUS       DACCKU010000171         2636 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.180, whole genome shotgun sequence.
ACCESSION   DACCKU010000171 DACCKU010000000
VERSION     DACCKU010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.180"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            5..862
                     /locus_tag="IVN13_002874"
     CDS             5..862
                     /locus_tag="IVN13_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP7013302.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            884..1393
                     /locus_tag="IVN13_002875"
     CDS             884..1393
                     /locus_tag="IVN13_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP7013303.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1430..1831
                     /locus_tag="IVN13_002876"
     CDS             1430..1831
                     /locus_tag="IVN13_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013304.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1943..2296
                     /locus_tag="IVN13_002877"
     CDS             1943..2296
                     /locus_tag="IVN13_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013305.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2330..>2636
                     /locus_tag="IVN13_002878"
     CDS             2330..>2636
                     /locus_tag="IVN13_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7013306.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     "
BASE COUNT          901 a          410 c          586 g          739 t
ORIGIN      
        1 ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca cgaaaaaaga
       61 tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact cttttgaacg
      121 ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac tttataaaga
      181 tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca ctcaattaag
      241 ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa gagcagaaga
      301 agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca cgaaagcggg
      361 attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg tccaatatat
      421 ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg cactttttcc
      481 ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa gacaagcttt
      541 cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga cagcgattga
      601 aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact atgtaaaagt
      661 agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg aaattcttga
      721 tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact tgttctatac
      781 gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat taattgtgct
      841 agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg tagtcaattt
      901 agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat ctcaaaaaag
      961 tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat ttatgagaga
     1021 tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta caccagagaa
     1081 agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt tgatcgcaaa
     1141 aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag aattgaatat
     1201 tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat ctgtttattt
     1261 aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg taaagaatat
     1321 ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat tgaacaagtt
     1381 tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa tgaagaagaa
     1441 cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc gccgtacaaa
     1501 aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta tgagaacaga
     1561 tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt atgttggaga
     1621 tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc atgcattagg
     1681 aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact ctatattatt
     1741 gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata acaaaaatcc
     1801 tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag catttgggag
     1861 ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa cttttattcg
     1921 aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg atccgagtgt
     1981 agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca gcgtgagatc
     2041 gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag atacaccgct
     2101 acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag ttgataagtt
     2161 gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc agttcttcta
     2221 tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca atcagattat
     2281 tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa tgttggaaat
     2341 catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg aagtaattgt
     2401 cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca tccaaacatt
     2461 cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca tgctagtatt
     2521 tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca atgaactgac
     2581 tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa tgttgt
//
LOCUS       DACCKU010000172         2582 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.181, whole genome shotgun sequence.
ACCESSION   DACCKU010000172 DACCKU010000000
VERSION     DACCKU010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.181"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IVN13_002879"
     CDS             <1..704
                     /locus_tag="IVN13_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7013307.1"
                     /translation="EETQGNVIVIDNDTFKQQHPNFDELAKLYEKDVVKHVTPYSNRM
                     TEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTI
                     ERYETMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSS
                     LETPSISPKETLERELNRKVSGKEIQPTLERIEQKMLQNQHQETPEFKAIQQKMESLQ
                     PPTPPIPKTPKLPGL"
     gene            1145..1462
                     /locus_tag="IVN13_002880"
     CDS             1145..1462
                     /locus_tag="IVN13_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013308.1"
                     /translation="MEKTKKLQLEDFTENEFYGTQEQQYLKAQVREELKEQGFIIDSS
                     FEGDFKTWIGVYARPKDKPTYLDPQNDKEAEEQEQYSINGFKQDFSEWFEWEIKNLKI
                     KEM"
     gene            1996..2493
                     /locus_tag="IVN13_002881"
     CDS             1996..2493
                     /locus_tag="IVN13_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP7013309.1"
                     /translation="MKYIKNVETLEELKKAYKKLALKLHPDCGGNEEEMKILNNEYDE
                     LFSKLKNTHKNKEGETYTKETTETPEQFKDIINKLFNLKMDGVAIEVVGTFIWLTGNT
                     KPYKDDIKALEFRYSPKKYAWYKAPSDYKKRSRKNYDMDTIRGMYGSQKIKEEKEEKK
                     YLQAN"
BASE COUNT         1064 a          446 c          441 g          631 t
ORIGIN      
        1 ttgaagaaac acaagggaat gttattgtca ttgataatga tacctttaaa caacagcacc
       61 ctaattttga tgaactagcg aaactttatg agaaagacgt agtaaaacac gtcacccctt
      121 attctaatcg catgacagaa gcgatcataa gccgtttgag cgatcaaggc tataatttag
      181 tgattgaagg tacaggacga acaacagacg ttcctattca aaccgcaaca atgcttcaag
      241 ccaaaggtta tgaaacaaaa atgtatgtta tggcagtacc taaaatcaac tcatatttag
      301 gaacaattga acgatatgaa actatgtatg cagatgatcc aatgacagcc agggcaacac
      361 caaaacaagc gcatgatatt gttgtcaaaa acttaccgac caatttagaa acccttcata
      421 aaacgggctt atttagcgat ataaggcttt acaatagaga aggagtgaaa ctctattcaa
      481 gcttagaaac gccttctatt agtccgaaag agaccttaga aagagaatta aatcgtaaag
      541 tatcagggaa agaaattcaa ccgactttgg agagaataga gcaaaaaatg cttcaaaatc
      601 aacaccaaga aacccctgaa tttaaagcaa ttcaacaaaa aatggaaagc ttgcagccac
      661 ctacaccacc aatacccaaa acacctaaac ttccaggact ttaaacctta aaaaaggaaa
      721 agagtagtta ccaaaaaacg gtaactactc tttttttata aaaacatttc ctcatgtttt
      781 aaaagctgat cgagaaataa agcacacaac ggtattcttt cttttttcag ttggtacatt
      841 ttgcagtctg taacgtgtca tttactgttt gttaccttaa catatccagt cgaaaacaca
      901 agggtatata aacgagcaag ctcgcccttg tgttttcttt gtgtctatgt tgcgttctcg
      961 tcacagcaaa cgacacgaac acactggcga aaatgaacca acaaaacgaa aaaagaaagg
     1021 agcgtacact gtaatttatt tctagctttt aaaacatgag gaaaacagct tcacttcctc
     1081 aaacgcctaa caaaaatggt tgtcaaaaaa ctaaaaatca aaaaaaagaa aagaggaaaa
     1141 gaaaatggaa aaaacaaaaa aattacaatt agaagatttt acagaaaatg agttttatgg
     1201 aacacaagaa cagcaatatc taaaagctca agtaagagaa gagctaaaag agcaaggctt
     1261 tatcatagat agcagttttg agggcgattt taaaacatgg ataggtgtat acgcaagacc
     1321 aaaagataaa ccgacctact tagaccctca aaacgataaa gaagcagaag aacaagagca
     1381 atattctatt aacggcttta aacaagattt tagcgaatgg tttgagtggg aaataaaaaa
     1441 tttgaaaatt aaagaaatgt aaaagaaaag cctagcaaaa aaagctaggt ttttctttta
     1501 cataaaacac caaaaagaaa aacctaagaa ggataaaata agttgttcca cttgcggtgc
     1561 agttgctcct cgttccaaac ccaacttatt tctttgcaaa gatatttata tttgactaac
     1621 acctttcctc gaatggtaaa agctaaccgt tcgttactct acctcaaata ctttcttttt
     1681 tcagttggta cattttgcag tctgtaacgt gtcatttact gtttgttacc ttaacatatc
     1741 cagtcgaaaa cacaagggta tataaacgag caagctcgcc cttgtgtttt ctttgtgtct
     1801 atgttgcgtt ctcgccacag caaacgaaac gaacacactg gcgaaaatga accacctaaa
     1861 cgaaaaaaga aaggagcaag atatgtagaa cactcacagc ttttaccatt cgaggaaaac
     1921 agcttcactt cctcaaacgc ctaacaaaaa tggttgtcaa aaagccaaaa atcaaaaaga
     1981 aaaggaatga taaaaatgaa atacattaaa aacgttgaaa cattggaaga attgaaaaaa
     2041 gcctacaaaa agttagcttt gaagttacac cccgattgtg gcgggaatga agaagaaatg
     2101 aaaattttga acaatgaata tgacgaactg tttagcaaac ttaaaaacac tcataagaat
     2161 aaagaaggtg aaacttacac caaagaaaca acagaaacac ccgaacagtt taaggatatt
     2221 attaataaat tgttcaatct aaaaatggac ggcgtagcaa tcgaagtagt aggaacattt
     2281 atatggctaa caggtaatac aaaaccttat aaggacgaca taaaagccct agaatttcgt
     2341 tattcaccaa aaaaatacgc atggtataaa gcaccaagcg attataaaaa acgtagtcga
     2401 aaaaattatg atatggacac aataagggga atgtatggaa gccaaaaaat taaagaagaa
     2461 aaagaagaaa aaaaatactt acaagccaat taaggcttgt aagtttatca gaaaggaaga
     2521 aaccattatg ttttataaaa taagaataat tgaaactttg gaaaaggaat ttgaaattga
     2581 ag
//
LOCUS       DACCKU010000173         2532 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.182, whole genome shotgun sequence.
ACCESSION   DACCKU010000173 DACCKU010000000
VERSION     DACCKU010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.182"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..254)
                     /locus_tag="IVN13_002882"
     CDS             complement(<1..254)
                     /locus_tag="IVN13_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7013310.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTS"
     gene            complement(366..1709)
                     /locus_tag="IVN13_002883"
     CDS             complement(366..1709)
                     /locus_tag="IVN13_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP7013311.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(1843..>2532)
                     /locus_tag="IVN13_002884"
     CDS             complement(1843..>2532)
                     /locus_tag="IVN13_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP7013312.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT          748 a          452 c          328 g         1004 t
ORIGIN      
        1 gaagtcgtac tactttcact tgatgaagtt gttgaagact cagcatttga agtcgtacta
       61 ctttcacttg ataaagttgt caaaagttca ctactctctg ctgttacatc ggaattactc
      121 atttcagtcg tctcggttct ttcttttgca attttgtatg ttgaattttc gcttgccaac
      181 acatggaaag gtgttgctat aaagagttgg gacattaata taactgctga tgcaagtttt
      241 aaagattttt tcatttgatt tacctctctt ttttctcttt aatagtgttt tctattaaaa
      301 attatgataa atattttcaa tgaattgagt aatacagtat atttcttgat ttcatttagt
      361 aacgattatg tttcaatgtt taatcctgtt actgccttta aaataaaatt atagggttgg
      421 ctaaaactag gatgaaataa ataatctaac attttcaatt tgtctaatgt aatttgttct
      481 tctatagcaa gagaaaacat tgataatagt ccagataaat cttcttgact acaaagttgt
      541 acacctacta ttctctttgt atttttttca aagctaattc ttaattttac acgactattt
      601 tttctcaaaa aaacaggtct aatgaaatct tctatttcta tataatctat agactcactt
      661 aataatcgtg ctgtttttaa attcaaacct gctgcgtaaa aattgtaatt aaatatagaa
      721 aagccatttg taaattggga accagaaggt gtaaaatctt gattgtcaat atgttgagtt
      781 gcaatataag ctgttcttag agcattagaa atagaaaaat caacactact tctattaatg
      841 gacgtaaaaa aagttgtagc acaatcccca atagcatata tatcttttat atttgtttgc
      901 tggtattcat tagttagata tgctccacta acatctctca agagttcctt tttgccaatt
      961 ggactattag atagaaatcc tatagcttgt acgaccatat ccatagagta ttgggtacca
     1021 cttgtagtga ctattttttc aatctttttt cttcctaaat attttttagg actttctgat
     1081 agacaaagat gtattccatt ttttgatagt atttcttcaa ctttatctga aaaactacgg
     1141 tcgtaataag tcgacaacac tctatctgca acatcgaaaa gatatacttc tttccctctt
     1201 tttcttatgg cttcagcaat ttcaactcca atatatcctg ctccaataat tcctactttt
     1261 ttaatagtat tatcatctaa agctttaaaa atctctttag catttggaaa attctttatc
     1321 gaaaaaatat tagctaaatt attccccggt acatctaaca taatttgctt tgaacctgta
     1381 gctagtataa gtttgtcata ttcttgggaa aatcttgtgc catctttttt tacacaaata
     1441 atctttttat tttgaatatc aattttgtca actgctgtct ccaaaaacaa cttttttatc
     1501 ttagaattta gttctcttct attttgataa aaaagatctt ctaaattgct aacttctctt
     1561 tttatgaaca acgatgtccc acattctaag taactaattt ctgatttttt atctatcaga
     1621 ataacttcat aatttgaatc acataagttt agaaatcgag ctgcagctag ccccgaatgg
     1681 tttgatccaa taattactat ttttttcact taatcacatt cttttccctt ctatgaaaag
     1741 tatcttcatt tttaactaac aacctatctc ccaaaataaa aaacaaaaag aggaagaaaa
     1801 agattcttcc tcttttcaga gaagatttaa gaaattgatt ttctattcat tctctttttt
     1861 tcgattttta taccctaata accctaaagt tcctaacagg attaaaccac cactaatagt
     1921 aaagatatta ctatcaatct ctcccgtctt aggataagct tttgaggact gcttttgagg
     1981 actgcttttg cctctatttt tgttttgctt gctaccttga ttgtcagtct tattatcttt
     2041 actaacggct ttttcactgt ctttttcgtt tgaagaaaca actacattag taggttcact
     2101 agtattacca gattcatcag ttgcagttac tatcactttt gttcctgcct ctaatttagg
     2161 aactttaact gtaaaattac catttttatc agctgtccca tggtattgac tcccgtttgg
     2221 taatgtaact gtcacctctg catttggttc agttttacca gtaacaattt ggtcaccctc
     2281 tgtaatagga tcaacttgag gtttattcgg tgctttttta tcttctacag aaatagtaac
     2341 ctctacttct tctttagatc catctggata tgttactact acaatccctt ttttgtttcc
     2401 agctgtacta acatctactt tctctttcca agtataagta gttccttttg gtagatcttc
     2461 tttgttctta attccctcag atgcgtctgg ctctttattt aagtctgtag tcactttttg
     2521 tccttcagga tt
//
LOCUS       DACCKU010000174         2523 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.183, whole genome shotgun sequence.
ACCESSION   DACCKU010000174 DACCKU010000000
VERSION     DACCKU010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.183"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..222
                     /locus_tag="IVN13_002885"
     CDS             <1..222
                     /locus_tag="IVN13_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013313.1"
                     /translation="LTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVEGKIKSNQTK
                     KYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            241..1833
                     /locus_tag="IVN13_002886"
     CDS             241..1833
                     /locus_tag="IVN13_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013314.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWHYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKSLKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIESMD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            1854..2189
                     /locus_tag="IVN13_002887"
     CDS             1854..2189
                     /locus_tag="IVN13_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013315.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            2335..>2523
                     /locus_tag="IVN13_002888"
     CDS             2335..>2523
                     /locus_tag="IVN13_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013316.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYPF"
BASE COUNT          998 a          390 c          519 g          616 t
ORIGIN      
        1 ttaaccgaaa tggtttcaga gtttgcggat cttcatttga aagatacagg aatactaatt
       61 ggtacaaatt caactgttta tcaagaagca gaagaacgtg tggaaggaaa aattaaatcc
      121 aatcaaacca aaaaatattc ttctaagtta aagaaaccag tgaatgaatc tgttcatacc
      181 agagtagatc catttgatcc aacgaagatt ctaaaaagat aaaagtagaa ggagaacgtg
      241 atgaataaat cgcctgcagt tattcttact tgtcaattta ccttgcccaa taaaaaatct
      301 ttttcaacat acattgatta tatgacaaga gaaaaagcac tggaagaaat ggaacgacgt
      361 acgcccgaag aagaacagga actgcaacga atcaagaatg ctttagagga tttttatatt
      421 cctgaaggag aaactttttc ggaaagtaag acggataaag aattcagtga aacgtcgaat
      481 gaagctcaat cgttgatgga acatggaatc aattttgaca agttagagga acaagatttt
      541 accaaatatc tttcttatat gacccgtcac tatgcgttag aacaaaagaa agaactgacg
      601 caaagggaac aaaaagaata ccaacggttg aaccaagcaa tgaaaaaata tcaaacagag
      661 ccacataaaa aagcttcagg aaatcttcct ggagtttttt ctatggcttc agacgaagtg
      721 aaaggaagtg atctgaagga gatcaaacaa atcttccaaa gagggcaaca aaaaggctca
      781 atcatttatc aagatgttgt ctctcacgac aattcgtatc ttgaaaaaat gggcttgtac
      841 gatcccaaaa cagatacctt agatgaagaa ggattaaaag cagctggaag aaaaatgatg
      901 gagacattat tcgagaaaga acaattgaat gaaacaggtt actggatggc aacgattcac
      961 cgtaacacta agcatatcca tattcatttt gcagttgttg aaaaagaaaa tacgcgtaaa
     1021 ccttacacgg aggttgaaaa tggtgtgagt tatatcgtac cgaaaggtaa gagaagacaa
     1081 gcaacattag acgcaatgaa gaccaattat gtacatgagt tagaacgttt ttcttatgaa
     1141 aaggatcgaa tgattggtat ggagaaacga aatttattga cgcggaaaag tgatttgcgt
     1201 aacaccttaa caaaagtggt aaaagagcca gcgcgttacg atcgacacgc aatgaactta
     1261 ttaaatgaag tatataaaac cctacctgac aaacgttcgg aatggcatta tggggatgaa
     1321 aaacgaacca aactttcacc atacacaaga gagaaactcg atgagttaac caaatatgtg
     1381 ttaacaaaag aacctgaata tcaagaatat atacagattt caaaatcatt gaaagaagaa
     1441 gcaaaaagtc tttatgggga aagcaagaga gaatcaaagg atgcagaaaa aaatgctctc
     1501 tttgatttga agaagcgcac aggaaatgcc attctaacag aactgaagaa acaggatagt
     1561 caaataaaac ctttaataga aagtatggat cagatgcaat ctatttacaa tgaacgatcc
     1621 tttcgaacgc ttaaacagtt accaacgaat gaatacaaat atagttacga agaattcatg
     1681 aagcgcagga aacaattcca acaaagagtt gtcacgcaac ggagtttgcg tcaattaaat
     1741 cgaacgatta atgcccatga agaaaaatac cacgcaagta aagcctacga agagcaacaa
     1801 cgaagaattc aaatggaaca agaaagacag taaagacagt aggaggaata gcaatgaaac
     1861 aatatttaga tcaatggaaa gttatagaag gatcattaag agaagaacga atcgaacaac
     1921 ttcccgattg tttagaaaaa gagcatttgt ttcagataag agaaatgttg agaaacgaac
     1981 agtttgatcc aaatcaattt ttagtcgttg agtactcagc tactggtgtg tactgttgta
     2041 atcatgtgaa gggtgaaaaa tattttatca ttcaagaata tgagggcaag ttagcgcctt
     2101 attatactac ttgggaaatg aatgaagagg ggatcaataa cttcccgtgt aaaagcattg
     2161 aagagtctat ttcactcaca gaatgttagt tgttaagtga ataaaagaag taaaagagtc
     2221 ctgaacgtaa ttgtccagga ctcttttttt caatgaattg ttttccgcca attcaatgag
     2281 tggtgcgatt tgcgactaca ttatatcaaa agaaagagaa gggaggaaga agaaatggct
     2341 tattcaacga ttggagttgt tgttgaaaaa agcagagaca atctcgtttt tgtgacagaa
     2401 attcaaacgg gcagagcttt tgtcgtcaca gacaaagcgg caaaagcgta tcaaagtggc
     2461 gatattttaa cgttaaatct gactacgaag acgttcgtcg atgcggcaga agattatcca
     2521 ttt
//
LOCUS       DACCKU010000175         2474 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.184, whole genome shotgun sequence.
ACCESSION   DACCKU010000175 DACCKU010000000
VERSION     DACCKU010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.184"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            960..1928
                     /locus_tag="IVN13_002889"
     CDS             960..1928
                     /locus_tag="IVN13_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP7013317.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            1945..2469
                     /locus_tag="IVN13_002890"
     CDS             1945..2469
                     /locus_tag="IVN13_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP7013318.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
BASE COUNT          945 a          420 c          427 g          682 t
ORIGIN      
        1 cactttagtg agttttgtag aaagtacctt taaaggtact ttcacattac gaacgatacg
       61 tccgtaggac gtactgaaag ggtgggattt atgagcgaac taatgcaaaa tttggctagc
      121 aaaaaaagtt aaaaaatata tgctaataat cgagcgaaat gaacactttt tcaagtgagc
      181 gaatccgcca tgacaaagct gaagcagttt atttcagctt tgtcatggcg tgtgcctaga
      241 cttttaaaaa agtcggcaca cgagcaaata tctttatcaa gctctaattt gcgttttaag
      301 acgtttgaaa cgtctaagta tataaatata taagagtagg tttaagagtg cttaaatcga
      361 attttagagg gcattcagac aactttaggg ttgttagggc ttgccctgac cgcctgtaag
      421 gcgctttaag aacaagataa gggatttata ctaagactgt cttaaaacgt ctcaaacgaa
      481 ggctcatagc ttctcaaaga gttccttatg aacattttta gcaaaggagc cggaagcgac
      541 tgcgcagtat aatgtgagag cagagcgatc acaccttttt aagcgactgt agggagtaag
      601 attcccagtt gagaaaaatg cgcgaatctg tttggagaaa aaagaagtgg ttttttaggc
      661 gggaaatttt gtccccctct tttataatct ctttttaaac ctctttttaa acctctttac
      721 cgtcggctat atccttactc tcccaaggga atgacgcttt tatctacatc gttttgtctg
      781 tttatctaca tcgttttgtc tgtttatcta catcgttttg tctgtttatc tacatcgttt
      841 tgtctgtttg tacattgaat tagtacatcg acgatgttaa tataagatgt agataaacag
      901 gcattttgta tttcataacg tgtatctctt gtgcgagtaa gaaaggagct attttaaata
      961 tgtctaatga aatcgtaaag tatcatcatg aattaaatac aattccgttg cgaaaattta
     1021 caccagtcga aatgaatttg ttcttttcta tcgtatcaag aatgcgtgat gtaggtgata
     1081 aaaaagtaca atttacattc gagcaattaa aagatttaag caattataaa gcaactgcaa
     1141 atgttcgatt catagatgat ttagaaacaa cgtatgacaa attaatggat ttacgttttg
     1201 gtagaagaag tgctgacggt ttgcaacgtg aacgttttgt tttatttaat caatttaaaa
     1261 tcgacggaaa atctgatatt ccttttgctg aaatacaagt tcatgaaaaa gccttaccct
     1321 tacttaataa tcttgaagaa tgggtgcgct attctctcca acaattcaat gaattagaaa
     1381 gcagttattc aaaaacaatg tttcgattac taaaacaata cagaaccaaa ggtttcgctt
     1441 atttttcaaa agaagacttt catgaattac ttgatattcc taaaagttac aaccaaggta
     1501 cgattgatca acgtgtgttc aatccaatac gacaagaact aaccgcaatc tttaaaggtt
     1561 taacaatcaa aaagaaatac ggaaaaggac ggggaaagcc tgtcattggc tatcaattta
     1621 catttaaacc tgagataaaa aaatcagacg actttaacaa acatgcccca acaaaagaag
     1681 aacaaaacaa agcattagaa ctagctaacc aacaagttca atctgaaaaa gcacctactt
     1741 cattacaaga aaaaatcaaa gaacaaaagc gaaaattgga acttttacaa cagttagaaa
     1801 agttagaaag agaacaaaat acccaaaata aagaaaataa tgcacaaaaa tcagagataa
     1861 attccgatat tccttcagaa ctaaaaaaag acttattaga caatcttcaa aacttattca
     1921 aagattgagg ggtttttaga ttttatgaca gaaaaaacaa tcaaagaact tgctgaagaa
     1981 ttaggagtta gtaaaacagc aattaacaaa aaagtttctg acgaaaatcg aaaactttgg
     2041 ttttcgaaaa tcggaaacaa gtttgtgatt aatgaaaccg gacaaaaggc tataaaatca
     2101 atgtttttta acgaaaacga aaactcaaat caacaactag ttggcgaaaa aacgaaaacc
     2161 gaaaaccaca aaaacgccaa ccaagttccc gaattagaag tcctttctct cctagaacaa
     2221 cagttaaatc aaaaagatat gcaactacaa gaaaaagacg agcaaataaa acaaatgcaa
     2281 aaattactcg accaccaaca acaactcacc ctgcaagcca accaacaaat cgagaaactg
     2341 caggaacaac tacagttgac ttatacggaa gaatcttcgg aaaataaatc aacgactctt
     2401 tctgaagaaa cagaatcgat agaaaaacaa tcaaaggcgg agaaaaaatg gtggcaattc
     2461 tggcaataaa acca
//
LOCUS       DACCKU010000176         2465 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.185, whole genome shotgun sequence.
ACCESSION   DACCKU010000176 DACCKU010000000
VERSION     DACCKU010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.185"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            669..998
                     /locus_tag="IVN13_002891"
     CDS             669..998
                     /locus_tag="IVN13_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013319.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            985..1326
                     /locus_tag="IVN13_002892"
     CDS             985..1326
                     /locus_tag="IVN13_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP7013320.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            1440..1679
                     /locus_tag="IVN13_002893"
     CDS             1440..1679
                     /locus_tag="IVN13_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013321.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            1746..1985
                     /locus_tag="IVN13_002894"
     CDS             1746..1985
                     /locus_tag="IVN13_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013322.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            2071..>2465
                     /locus_tag="IVN13_002895"
     CDS             2071..>2465
                     /locus_tag="IVN13_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7013323.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAG"
BASE COUNT          895 a          306 c          558 g          706 t
ORIGIN      
        1 aacggtgtac tgattggtcg aagaaaaaat ggagtcttgc gttgtaccgc aaaagggatg
       61 gggcgtttga tcagctgaat tgatcattct cataacttgt attttatcgt ttttcgcttg
      121 cttgaaaata ttgtcgagac ttctaaagga acttagaatc attagaaaaa taaggagtag
      181 acttgatcta tcaacaattc ccttcgggga gtgacggggt aggtttttac gattggtaac
      241 gtaaaaaatt ggtcaatcga gtaaatgccc ttgtttggat agtggtgaaa tgaatttgtg
      301 taaataatat gcacagagta aacaagaggg aatttagaag ttagacaagt tagaatagcg
      361 cctagattaa tcatgagcag ctactaagga atctaggctt tgacgcaaac tcatggaata
      421 aggagtaggt cttctcgtaa tggggaggac gcacaagaaa ttgtgtcgtg tgctgcagta
      481 catgtagata cttgcgagga cgttgattat tggatcaatc gagcatttac cgctgtctac
      541 tagggtttct cgtttcggac gccataaacg agtcgattta ttttatatat ttttgagtga
      601 acgaaaaacg ataaagatat tcgttatgaa gtaaaactaa tttagtagat agcaaaggtg
      661 gtagaggaat gattcaaaaa tttgcgaaaa tgagtaaaac tgcatggatt gaaaagcttg
      721 aaaatgactt gagtgtcatg cgattagctc caaagaaatt caaacgagag attgcagaaa
      781 aagaagcaat tttgtcggta ttgaatgcgg tagacgataa atgtccagta aaaattagtt
      841 tttcagatat agcaacaatt atcgaaggta tttattatgg tgaagttgat gaagagatat
      901 cttctacaaa agatttatta gattttttgg ataaaaacaa agataccttt atagtactaa
      961 ctgattatga ggaggttaca acaaatgaaa aattatgatc cgaatagccg atggggaaca
     1021 catacgatta aggtttcttt ccaacgttgg gattacaaag gatttgtgac gtttagaaga
     1081 ggcggaaatt gtaaaggatt agatgtttta gcattagacg aggatgattt atacgatcaa
     1141 aaattgactg ataatccaat tggattcggc ttgctacctg aagatgatca aggtaatgaa
     1201 tggtttaaaa tgacgcttat gaatgataat ggagacgaat tatctgttga agatacttgg
     1261 tcatatttga gtgattatat tgtcagcgtt gaaatcattg aatttgtagc ggataaagaa
     1321 gaataaaaac tggaggaata aattttacag ataaaggctt agaaggagtc acgacgatta
     1381 ataaaaaggt agtattacct taggcattga gtaaggaatt tatttaggag gaaaattaga
     1441 tggcgcgacc agttttagag gcgatggata tggatacttt gacaaaagga aggtatgtac
     1501 agctgttagc tcgtggttat tcacctaaag aagtattcaa atccctgtct aaaggaacag
     1561 atatggaaaa agaaagatta cgaaaagaat tcgattattg gagaacccat aatggaatca
     1621 aggatttgaa accagcacgg ccagtaatta aacgaaagaa aaagataacc caagactgaa
     1681 gtgtagagcg tatgtgatca taggcttttt tatttttgca aaaaacaagg aaggtaggaa
     1741 aactcatgaa ttatgaagaa atcgaaaaca gaaaaaaagt gtcgaaagaa atggaagaaa
     1801 agttgctaaa aacgatgaaa caaaaacacc ttaaaagatt atctgtcatg caatatatca
     1861 atgatatgca gataacagga aaagagaaag cttgtttgtt aggttcaatg aaaaactttg
     1921 aacagttgag acgaacttat gtgaaaaaaa gttctaattg tcaactattg ttagaagtct
     1981 catgacattc attaatagaa ttcattttta aaactttttc tatggaaatg agaaaaagat
     2041 tttttgtttt gtctgaaagg gggtagctgg atgagaaaat ggttatttgg ttttttagga
     2101 gtcgctttaa tcgtcttttg cagtgtgttt ggctatgtta gctaccaaaa acatgaagga
     2161 aaggtgttta agcaaaatat agaaaaaaag atgcccgtag atcaaatcaa tgaacatgca
     2221 aagagttata aggaggatgc aacgaacgtc aataatgata tgtctttggg gcaaatgttg
     2281 tccatacaaa aagaagcaat cgaaatgggt gtcaataaac aagcctttgc acaaattcag
     2341 atacctgcac ttggtttagc attgccaatt tttaaagggg caaatcaata cactttgagt
     2401 cttggagcgg ctacttattt ttatgaagat gccgaaatgg gcaaaggaaa ctatgtccta
     2461 gcagg
//
LOCUS       DACCKU010000177         2446 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.186, whole genome shotgun sequence.
ACCESSION   DACCKU010000177 DACCKU010000000
VERSION     DACCKU010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.186"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            48..2024
                     /locus_tag="IVN13_002896"
     CDS             48..2024
                     /locus_tag="IVN13_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7013324.1"
                     /translation="MAINRYVKKAGMIVGMLGIIAGCFQGYRAYAEDVTQKTPPERVN
                     ITVHKLMYDQSTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGELVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGEEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGAISAVVYFKKKA"
     gene            2037..>2446
                     /locus_tag="IVN13_002897"
     CDS             2037..>2446
                     /locus_tag="IVN13_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP7013325.1"
                     /translation="MIIRHPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSV"
BASE COUNT          880 a          392 c          536 g          638 t
ORIGIN      
        1 atttaatcaa cgcgagtcgt tgaaatatga aatgaggaga gtaaaaaatg gcaataaatc
       61 ggtatgtaaa aaaagctgga atgattgttg gaatgttagg aatcattgca ggctgctttc
      121 aggggtatcg agcttatgca gaagatgtca cgcaaaaaac gccaccagaa agggtcaata
      181 tcacggttca taaattaatg tacgatcaaa gtacgcaatt gaacgttgat attgacggaa
      241 tcaaaaatga tggttatacg cacgatcaat atcctacagg tgtaacgaaa tacaacaagg
      301 cagattatgg tgatgttgag tttacgttag gaaatattac ggatcaagta ttaccgactg
      361 aagattctga tctaaccaac gcgaaagtgg acgaaatcgt taaagatgta gaagacaagg
      421 gatctaacag tgagtacgtt aagaatgcaa ccaataaaat tacttcagct gtagatgaaa
      481 atggtgagat cacttttgct gatcaaccag cctatgtcaa ttccaaaggg aatgtatatg
      541 tggtttatga aagtaaatcc gctgctggat tagtcacaca aaaagcaaaa ccaatggttg
      601 tgattgcgcc aatgacagac aatacaggat caggattttt aaaagatatt catttgtatc
      661 caaagaacat tgtttctaag ttgagttttg aattaacgaa atttggtgac gatggcacag
      721 ctcaaagcaa acaaacacct ttaaaagggg caaaatttga gttatacaag ggcgaacctg
      781 gaaaaggaac aaaattagga gagcttgtat cggatgatca aggaaagctg actgcaactg
      841 atttgacgtt aggaaaatac tactttgtgg aagttccttc agaagttgta gtgggatcag
      901 atcaagaacc aacagcagac caatatttgt taggcgcaga cgctcgcaac gatgcgcaca
      961 ataagttaac ctttgaaatt acgaatgacg gcgtgacaag tgatttaaaa gcttcctacg
     1021 tgaactataa agcaccagtg atcgataaaa cagtgacaaa tggaacagga gaagaacatt
     1081 ctttccaaat tggtgacgca gtgaattatc aaggtacaat tcatatccct acagatatcg
     1141 caggtggaac agatgggatt acagtaaatg gtgtaaaaag tgaaacttct ccttacagtg
     1201 tatttaaatg gggcgacaca gcaggtaaag gactttctta tgttgctgaa aaagcagata
     1261 ttaaagtaac aaataatgat ggttcagtcg tattaaaaga aaatactgac tataaaatcg
     1321 aaaattctga tagtggtttt gtgatcgatt ttatcgtaaa taatggacaa gtaagtgata
     1381 cagttgcgaa tttacatggt caagatctaa aaatgactta taacatgttt gtgaatgata
     1441 gtgctgaaat cgcaaatcca ttaacaaata gcgtggattt cgtttataac aataatccat
     1501 ttaatcagga agaacaccac gaaaaaacga aagctgacgt tgtgacttat ggcgcaaaat
     1561 tcttaaaagt cgatagcggc ctatttggaa caggtatcaa agcaacaccg cttgaaggcg
     1621 cagaatttgc agctaagaat gcagaaggta agtactacgg tggacttact gatacagata
     1681 aagatggtgt caaagaagca gtttgggtag acgatgtagc aaatgcagca atcctaaaat
     1741 cagataaaga gggtcatttt gaaatcactg gtttaactga aggagattac tcactggaag
     1801 aaacaaaagc tcctgaaaac tatcaaaaat taacaaaaga gatctctttc aaagtagaca
     1861 aagattcata caaagaagaa aaccgaatca cgatcaaaaa taatcaaaaa gcttccgtac
     1921 caatgactgg tagcaacggt ttccaatctt acgtattgat ctcttgcttg ttgcttgggg
     1981 caggcgcaat ttctgcagtt gtttacttta agaagaaagc ctagaaggat cagctaatga
     2041 ttatcagaca ccctaagaaa aagcgaatca tgggtaaatg gatcattgct ttctggttac
     2101 tttcagctgt tggtgtctta cttttgatgc ctgctgaagc gagcgtggcg aaataccaac
     2161 aaaatcagca gattgccgca atcgatcgaa caggaacggc agcagaaacg gattcttctt
     2221 tggatgttgc taagattgag ttaggcgatc ctgttggtat tcttacaatt ccaagtattt
     2281 ctttaaaact accaatttat gacggaactt ccgacaaaat tttagagaat ggtgtaggga
     2341 ttacggaagg aactggcgat attacaggag gaaacggtaa aaatccttta atcgcagggc
     2401 acagtggact ttataaagat aatcttttcg atgatttgcc ttcagt
//
LOCUS       DACCKU010000178         2438 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.187, whole genome shotgun sequence.
ACCESSION   DACCKU010000178 DACCKU010000000
VERSION     DACCKU010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.187"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            48..2024
                     /locus_tag="IVN13_002898"
     CDS             48..2024
                     /locus_tag="IVN13_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP7013326.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            2039..>2438
                     /locus_tag="IVN13_002899"
     CDS             2039..>2438
                     /locus_tag="IVN13_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HAP7013327.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDD"
BASE COUNT          887 a          392 c          535 g          624 t
ORIGIN      
        1 ttttaatcaa cgcgagtcgt tgaaatatga aatgaggaga gtaaaaaatg gcaataaatc
       61 ggtatgtaaa aaaagctgga atgcttgttg gaatgttagg aatcattgca agctgctttc
      121 aggggtatca agtacacgca gaagatgtca cgcaaaaaac accaccagag aaggtcaata
      181 tcacggttca taaattaatg tacgatcaag gaacgcaatt gaatgttgat attgacggga
      241 tcaaaaacga tggttatacg cacgatcaat atcctacagg tgtaacgaaa tacaacaagg
      301 cagattatgg tgatgttgag tttacgttag gaaatattac ggatcaagta ttaccgagag
      361 aagattctga tctaaccaac gcgaaagtgg acgagattgt acaagatgta gaagacaagg
      421 gatctaacag tgagtacgtc aagaatgcaa ccaacaaaat tacttcagct gtagatgaaa
      481 atggtgagat cacttttact gatcaaccag cctatgtcaa ttccaaaggg aatgtatatg
      541 tggtttatga aagtaaatcc gctgctggat tagtcacaca aaaagcaaaa ccaatggttg
      601 tgattgcgcc aatgacagat aaaacaggat caggattttt aaaaaatatt catttgtatc
      661 ctaagaacat tgtttctaag ttgagttttg aattaacgaa atttggtgac gatggcgcag
      721 cccaaagcaa acaaaccctt ttaaaagggg caaaatttga gttatacaag ggcgaacctg
      781 gaaaaggaac aaaattgggt gatcttgtat cggatgatca aggaaagctg actgcgactg
      841 atttgacttt aggaaaatac tattttgtgg aagttccttc ggaagttgta gtgggatcag
      901 atcaagaacc aacagcagat caatatttat taggcgcaga cgctcgcaac gatgcgcata
      961 ataaattaac ctttgaaatt accaatgacg gcgtgacaag tgacttaaaa gcttcctacg
     1021 tgaactataa agcaccagtg atcgataaaa cagtgacaaa tggaacagga caagaacatt
     1081 ctttccaaat tggtgacgca gtgaattatc aaggaacgat tcatatccct acagatattg
     1141 caggtggaac agatgggatt acagtaaatg gtgtgaagag tgaaacttct ccttacagcg
     1201 tatttaagtg gggagataca gcaggacaag gactttctta tgtcgctgct aaagcaaata
     1261 tcaaagtaac aaataaagat ggatctgtgg tattaaaaga aaatacagat tataaaatcc
     1321 aaaattcaga aaatggcttt gtgatcgatt ttatcgtgaa taatggccaa gtaagtgata
     1381 ctgtagcaag tttacacgga caagatcttc aaatgactta taacatgtat gtaaatgaca
     1441 gcgcagctgt tgccaatcca ttaacaaata gcgtggattt cgtttataac aacaatccat
     1501 ttaatcagga agaacaccac gaaaaaacga aagctgacgt tgtgacttat ggtgcaaaat
     1561 tcttaaaagt cgatagcgga ctatttggaa caggaatcaa agcaacaccg cttgaaggcg
     1621 cagaatttgc tgctaaaaac gcagaaggaa aatattatgg tggattagtt gatacggata
     1681 aagacggtgt caaagaagca gtatgggtag acgatgtagc aaatgcagcg atcctaaaat
     1741 cagataaaga aggtcacttt gaaatcactg gtttaactga aggtgaatat tcactggaag
     1801 aaacaaaagc gcctgaaaac tatcaaaaat taacgaaaga gatctctttc aaagtagaca
     1861 aagattcata caaagaagaa aaccgaatca cgatcaaaaa caatcaaaaa gcttccgtac
     1921 caatgaccgg tagcaacggt tttcaaacgt atgtattaat ctcttgcttg ttgctaggag
     1981 caggggcact ttctgcagtt gtttacttta agaagaaagc ctagaaggat caaaaccaat
     2041 gattatcagg aagcctaaga aaaagcgaat catgggtaaa tggatcattg ctttctggtt
     2101 actttcagct gtaggtgtct tacttttgat gcctgctgaa gcgagcgtgg cgaaatacca
     2161 acaaaatcag cagattgccg caatcgatcg aacaggaacg gcagcagaaa cggattcttc
     2221 tttggatgtt gctaagattg agttaggcga tcctgttggt attcttacaa ttctaagtat
     2281 ttctttaaaa ctaccaattt atgacggaac gtccgacaaa attttagaaa atggtgtagg
     2341 tattacggaa ggaactggtg atattacagg aggaaacggt aaaaatcctt taatcgcagg
     2401 tcatagtgga ctttataaag ataatctttt tgatgatt
//
LOCUS       DACCKU010000179         2399 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.188, whole genome shotgun sequence.
ACCESSION   DACCKU010000179 DACCKU010000000
VERSION     DACCKU010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.188"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            208..2070
                     /locus_tag="IVN13_002900"
     CDS             208..2070
                     /locus_tag="IVN13_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7013328.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2070..>2399
                     /locus_tag="IVN13_002901"
     CDS             2070..>2399
                     /locus_tag="IVN13_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP7013329.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaaatttg aaaaagcgtt tgattattta tattacgact tagtgcaata aaaaacgctt
       61 tttaacagta catgtagtcg tctttataat agataaactc tgtaatatta gttaaaaagc
      121 tataagttgc acttagttaa gtgcaaacat ttcatattgt cgttagttta gaaaacgctt
      181 atattagcaa tgtaaggaga gatgataatg cagcagctaa caaaaagaga aaggcagctt
      241 ttgatttact tggtggaaat gtctgaaccg gtaacaatca aaaagttggc tgaaaaagaa
      301 aatgttagtg ttcggacgat aaaatacgat ctagatgata taagagagtg gctgaatgag
      361 cggaagcaag tcctatatag caaacgtagt cagggaattt ggatgaatat tagtagctct
      421 gatcgaatca aattaaaaag cgaattgatg gatgttaatc gttttgaaat atttgcagat
      481 caaagtctcc gaatcaaacg tttactaatt atgcttttag taacaaaaga agctattact
      541 ggaatgaaaa tggccaacgc attagatgtc agcaaagata cgatcatgaa tgatttagat
      601 gtgctggaaa ataattttct taaggaagga ttgaccttga atcgtcaggc gcgaaagggt
      661 ttttggatca taggagaaga acgtctagtt cgattaacga ttgaagagat tcttcaaaaa
      721 gagttcacgg attatgacat ttataaatta atgtcattgt tattgaatgg cggtgagact
      781 gaatactttg aaatgtattc tgctactgct acacctatcc aagaggtatt taatcaagta
      841 atcattcgaa tgcgacactt actggaattt gaaaatctag agaaactgaa ttacgcggaa
      901 ttactaaata ttctgattcg agtgacaatt gctaccgttc gcttacgcaa agaggctacg
      961 attggtcgtt atcaattggt tgctgaacaa gaattagaca aaaacgatct ttcttatcgc
     1021 gtcatgcagc aggtctttga ttattatcag ttgccactgt tcgaggatga atatcattat
     1081 atctatagtg atacattcga cacgattcac cagcaagacg tcatgaaact agttgagaat
     1141 gtgatttata aagtaagtaa agcaatcaac cattcttttt atcaagatcc acaattgttg
     1201 acgaatctgt atgctcattt atccatgcga ttatcgagaa agaaaaaatt tgtcaatgaa
     1261 tataatcctt ttaaagaaga tatcaagcag aaatacccgg aattatttga tgcgatcaag
     1321 caagcagttc gacaagaaat aagtgaacaa aatattctga tcaatgattc tttcgtggca
     1381 tatattgcct tacacttttt ggtttcctac gaaagagaag aagatctacg aagcgttaga
     1441 gccgtctaca tttgttcaac agggttgggt gtgactagtc tgatcaagca aaagatcaat
     1501 gaagaattaa ataatatcac tatcgtggca tttgcttcag tattgaatgc gcaagaaatg
     1561 attgctacca aagatcctga tttggtgatt agtatttttc caattaatgg catcgatcgt
     1621 cctttcatca aagtccatcc attacccact gaacaggact tgaaaaaaat tcaacaaata
     1681 ataaaaaaaa tacttgcttc ttccaatcag caggaaaggc ataacttgaa tcttagagaa
     1741 acgtccatca ataaagatac tttagaagac tttagcaagg aattgatttt gaaagcttat
     1801 acgatttatg aacgtttact tgtcattttg gaagcagaat tgatccaaga atatcgagaa
     1861 gcttttttgc ttcatgtatt cttaatggtt catcgtatca catttgatca acaatatatg
     1921 gcagaaggtc gtcaagtaga ccagattatt ttagacaaag gaacactggt aggaaaaatc
     1981 gaagcagttt ttgcagaaaa taatttagga atcaatcagt cagaaatcag tgcattattc
     2041 tcatatataa aagaaggagc gtcaagttag tgaaaatgtc agacgaggct ttaaaaatta
     2101 tcgaggaaag tcaaaatcgg gaaaagttaa aacaagtgat tgatctaatt gaagagcgat
     2161 tagtagaaca tgctattgta ccaaccgagt tgcagtggac cattctaatt aatcacttga
     2221 atgagatgct gaaacgttct caaagaaagg aaacgattcc cgtagtcgat cgggatttgt
     2281 tcaaagaagt atccaaagaa gccataacta tttctgatga aatagttaga agtatcggta
     2341 atttaaccga tgatgaaata tatgttttgt cgattcattt tgaaacagca aaaaataat
//
LOCUS       DACCKU010000180         2365 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.189, whole genome shotgun sequence.
ACCESSION   DACCKU010000180 DACCKU010000000
VERSION     DACCKU010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.189"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..62)
                     /locus_tag="IVN13_002902"
     CDS             complement(<1..62)
                     /locus_tag="IVN13_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP7013330.1"
                     /translation="MNHFKGKQFKKDVIIVAVGY"
     gene            complement(136..417)
                     /locus_tag="IVN13_002903"
                     /pseudo
     CDS             complement(136..417)
                     /locus_tag="IVN13_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            879..2261
                     /locus_tag="IVN13_002904"
                     /pseudo
     CDS             879..2261
                     /locus_tag="IVN13_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          862 a          404 c          392 g          707 t
ORIGIN      
        1 tagtaaccaa cagcgacaat aatgacgtct tttttgaatt gtttgccttt aaaatgattc
       61 attactctgc cctctctgtc ttttttctca attttacact aaaatagatt ttttggaaaa
      121 ctttgcaaca gaaccgaaat gagtatagta tgaattagta atttggtttt catctaagtt
      181 tagctttgat ttactaaaat tgatatctcg atacatgttc aatacatcta atacaaatcg
      241 agaatcttct ttcggaaatg gctcagaaag atcactccaa atttcatcat cataatgata
      301 ctcgtaacct tctctcaata tttcgatttt tctttcataa taagatctgt tttggtcctc
      361 aaatttagcc aataactcaa attgatttac tagtgacaat ctctgaaaac tgtccattaa
      421 atgtttcttc ctatcttgtt cattaacaaa aaattataag cccttacaac gaatattata
      481 tattaaattt aattgtaggt gaacacgaat tttataaata tttatggtgt taatgacaaa
      541 tgatatccga ctcctcttca tacgttctgg actatttgct aatagattaa aatttcagca
      601 tctatttctg accataaata aatgagctgc cgaaaagctg ccaaatcaga cttgaaaata
      661 aaggatcaaa aacttttaag tttctgaccc cgtaactcac acttcaaaat cttacttaaa
      721 aaggagtctg aaagaaaaaa agagtattca aaattttttt ctaactagct acatcacatc
      781 tatgcattga cactcaaaga accagaataa gtcatcagaa agcaaggggg tgcggacata
      841 atcctggacg aaaaaataaa cttaggagga atgtccgtgt gttttcccac aaagagcgtg
      901 taagggcaat tcagttattt ttaaaatacg attgctctta tgctgcaacc attcgagaat
      961 tagggtatcc gtcgattggt gcactacgga aatggtataa agagtatttg gaatccggag
     1021 agctccatca ggagcaccga aaaaaatcaa agtattctga agaacaaaaa cgaatagcgg
     1081 tgaatcatta ttttgaatat ggtcaatgtt atgcacgaac cattcgcatg cttggttatc
     1141 ctaatagaga gtccttgcgg cagcggtgtg aagaacttgc accaggagct agaaaactcc
     1201 gaaaaagtgc ggtaaagtta acacaagatc aaaaagaagc tgtgttgaaa aaattctata
     1261 aaccgcaaac taataggaaa aatttagccg aatctggagg aattagtaga gttactctat
     1321 atcaatggaa aaacacggtt cttggaaagg attttccatt gtgtatgact ttaaaaaatc
     1381 aggaaaaaca aaaagagcta cttttaaagg aagtagaaga cttacaaaaa caagtacacc
     1441 aattacagct cgaaaaagca ttactcgaag gagcggctga actgctaaaa aaagaaaaag
     1501 gcgtcaatct tctttgtcta tcaaatcaag aaaaaacgat attgattgat gccttacgaa
     1561 atcagttcac cttgaaagag cttcttcaac agctccagtt tcctaaaagc agctattttt
     1621 atcaaaaaca agcacttgag aaaccagata agtattacaa agaacgtcaa attaattatc
     1681 acgattttca atcataactt ctgtgcgtat ggttaccgta gaattcacca agctttaaaa
     1741 aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga ggaaaatcta
     1801 tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat ttctcctgca
     1861 caccccaatt tattaaatcg gaattttaaa gcaaagaagc caaatgaaaa atggttaaca
     1921 gatattactg aatttagaat tctcgcaggg aaaatctact tatcaccact tgtcgattgt
     1981 tacgatggtg ctattgttag ttggactatt ggaacaaagc caacttgctt cactctatgt
     2041 ctaaaaagag ttattcacca gataattcag cgtgcgaagg tttctttgga cgattaaaaa
     2101 acgagatgtt ttatcaacgt gactggaaga atacaacaat caatcagttt ataaacaaat
     2161 tggatgatta tattcattgg tataataatc aacgaattaa gttatcttta ggaaggctaa
     2221 gccctcttca atatcggaag aagcaaagat atatttgtta aaaaggtcca aaaaaaatat
     2281 ccgcaccccc ttttttttgt aaaaaactat tgcacaatac aagtagccta cggctatcga
     2341 aatttccata actttcgagc aagaa
//
LOCUS       DACCKU010000181         2358 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.190, whole genome shotgun sequence.
ACCESSION   DACCKU010000181 DACCKU010000000
VERSION     DACCKU010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.190"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..669)
                     /locus_tag="IVN13_002905"
     CDS             complement(<1..669)
                     /locus_tag="IVN13_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1062 family transposase"
                     /protein_id="HAP7013331.1"
                     /translation="MTYTHLTSNKLAMIEAYYNNHQSVAKTAVLLNRSRQTIHKVYQF
                     FKTGHNALDYFNQYKKNKTRCGRRPIVLSDKQTEYIQKRVVQGWTPDVIVGRAEFSIS
                     CSMRTLYRMFKQGVFEVTHLPMKGKRKANGHKETRGKQSFRRSLRDRGNDYSKFNQEF
                     GHLEGDTIVGKKHKSAVITLVERLSKVIITLQPEGRRAIDIENRLNQWMQSVPKHLFK
                     SMTFR"
     gene            complement(751..2205)
                     /locus_tag="IVN13_002906"
                     /pseudo
     CDS             complement(751..2205)
                     /locus_tag="IVN13_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311502.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
BASE COUNT          765 a          485 c          371 g          737 t
ORIGIN      
        1 gcgaaaagtc attgatttga atagatgctt aggtactgat tgcatccatt ggttcaaacg
       61 attttcaata tctatagctc gtctgccttc tggctgaaga gtgatgatta ctttagataa
      121 tcgctcaacg agggtaataa cagcactttt gtgcttttta cccacaatag tatccccttc
      181 aaggtggcca aattcttgat tgaatttaga ataatcattt cctctgtcac gaagtgatcg
      241 acgaaaagat tgttttcccc gagtttcttt gtgtccgttg gctttacgtt ttcctttcat
      301 aggtaggtga gtcacttcaa atacgccttg tttaaacata cgataaagtg tacgcataga
      361 acaagaaata gaaaactctg cacggccaac aatcacatca ggtgtccaac cttgaacaac
      421 cctcttttga atatattctg tttgtttatc tgataagaca ataggacgtc tgccacagcg
      481 agttttattc ttcttgtatt gattgaaata atctagagca ttgtgccctg ttttgaaaaa
      541 ttggtaaact ttatgaatcg tttgtctaga tctattcaat agtactgcgg ttttggctac
      601 agattgatga ttattataat acgcctctat cattgcaagt ttatttgatg taagatgggt
      661 ataggtcatt tgtcttcact ccttataatt cttttgcggg aactatcttg agtgtagcat
      721 aaatgacttt tttatttgtc tagcttaatt ttacaatcgg cgattgtcac ttgcaatatt
      781 tactggaatt tcgctcgtat cttcttttac ttcctcagtc tcttcatcaa tacctaaaat
      841 gaatgaagca ataaaaccga caacaaatgc aatcgcacaa ccaatcgctg cgtaaataaa
      901 atttgatagt cctttttccc caaagaaact tggtaacgca agaatgccag gagcaacttg
      961 agcatatcga ccgacaccca tgattccaat aaatagtccc gctgctccac cacctaccat
     1021 tgaagcaatt aacggctttt tgaatcgcat attaattccg tataaagctg gttcagtaat
     1081 tcccataaaa gcagacagac ctgaagaaac agctaattgt ttgacagaaa gttgttttgt
     1141 tctaaaggca actgctaagg cagcaccacc ctgagcaata ttggaaacca tcatacctgg
     1201 accagcaaca gtatcaaatc cagatgttac taacatatta ataccgattg gaattaaacc
     1261 gtaatgcata cctgtcatca caagtaatgg agtaaatgca cccacaagta atggaaccaa
     1321 ccaacttgca tacgtattaa caaattcaat aagtgaccct aacccattac ctaagaatgt
     1381 acccaaagga ccaatcacaa ttaaggtaat tggtgcaaca atcaacaatg ttaacagagg
     1441 cgtcataaat attcgaacag cattgggcat caatttatcg ataatcggct cgatatatga
     1501 cataaatcaa atagctaaaa taatcggaat aacagaagaa ccatagttag ctaacgtcac
     1561 aggtaatccg aataaagcta aggactcacc tgtttctcta gcacccgcaa tcattgctgt
     1621 aaaagtcgga tgtaacaaaa ttgctcctaa ggtaattgca atgtactgat tacatccgaa
     1681 tttttttgca gcagaactag ctaataagac cggtaaaaaa taaaaggctg catcaccaat
     1741 gaaatttaaa aattgatagg tttgcgattc tggtgtcatc cattccaaca tgactaatag
     1801 agctaataat gcttttaaca tccctgcacc tgttaatgcc ggaacaattg gaacaaaaat
     1861 acccgcaatt gtatctaaaa cttttgatac aatcccttta ttgtttgctt ctgaactact
     1921 ttctttagaa gcggaaaaat ctcctagttt atcgatttcg gcaaatacaa attttacatc
     1981 tccaccaata atgacttgaa attgaccacc ctgattaact acacctacaa cacctttaag
     2041 atttctaata gctgattcat ctgctttact gtcatctttt aaattaaatc gaagtcgtgt
     2101 tgcacaatgc gctaaactgc ttacattttc agaaccacca acatttgcta atatgtcttt
     2161 tcctaattct ttatacccca tgctactttc ctccttagag aacattttct cttttgataa
     2221 tttgaatata gcgtgctatt aatagataaa caatacattt gaaaccgttt tacacagcgt
     2281 tgcgaaattc attcagttta cgattatttt ttcgcaacac tgttccaaaa aaataaaatc
     2341 cacttactaa ctaatcgt
//
LOCUS       DACCKU010000182         2350 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.191, whole genome shotgun sequence.
ACCESSION   DACCKU010000182 DACCKU010000000
VERSION     DACCKU010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.191"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..544
                     /locus_tag="IVN13_002907"
     CDS             <1..544
                     /locus_tag="IVN13_002907"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7013332.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(1017..1589)
                     /locus_tag="IVN13_002908"
     CDS             complement(1017..1589)
                     /locus_tag="IVN13_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP7013333.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1605..2210)
                     /locus_tag="IVN13_002909"
     CDS             complement(1605..2210)
                     /locus_tag="IVN13_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP7013334.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          741 a          468 c          358 g          783 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaat gtgtccaaga tattgactca aatccagtaa tatcacaata
      601 ggaatgctgt ttattatatt agcagcagtg ctgattttta tgatagttat ttggttctta
      661 aatgaaagat ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca
      721 atagattatt tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg
      781 aacgatatgt gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc
      841 taatatagta agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca
      901 cattttttga gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag
      961 actcatatct agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa
     1021 ctttcttcaa ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct
     1081 tcaacttcct tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt
     1141 gtgattgttg ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt
     1201 tttccttctt gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac
     1261 gtccgtgtca ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg
     1321 atcgttacgt tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga
     1381 gcaaatcgat caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc
     1441 tgattgaatt ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc
     1501 ttctctacgc agttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt
     1561 gagacacgtg catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc
     1621 gtatactcag aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg
     1681 cgatcgtcaa ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt
     1741 atgggacttc gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca
     1801 actttttgca attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt
     1861 ccttctcgaa atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa
     1921 gaagtttgtg gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca
     1981 aatcgaaaat ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata
     2041 tccgaaaata gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca
     2101 tttatttttc cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc
     2161 ttagccaact caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc
     2221 cttaacttta tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt
     2281 tatgtgtcaa aaactattaa gtttctgacc cccgatagat ttttcaagtg tctctatttt
     2341 gcacagtcta
//
LOCUS       DACCKU010000183         2340 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.192, whole genome shotgun sequence.
ACCESSION   DACCKU010000183 DACCKU010000000
VERSION     DACCKU010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.192"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(365..1657)
                     /locus_tag="IVN13_002910"
     CDS             complement(365..1657)
                     /locus_tag="IVN13_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7013335.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1931..2191)
                     /locus_tag="IVN13_002911"
     CDS             complement(1931..2191)
                     /locus_tag="IVN13_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013336.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          781 a          441 c          355 g          763 t
ORIGIN      
        1 tatgggctac tctgtgatac ttattagcca ttaaactatt ttacccacat agcatatatt
       61 ttgaaatcgt tcattccgta aattcaacta aatatgccta aaaaaataaa aaatctaaga
      121 tagaataaga tttattcagt cttagatgga tttatagcaa ctttttcgta acaactttaa
      181 aaaatacgaa aagaaagagc tgttttactc tgactacgtt gctaaattgt cgtttaatca
      241 ttcatttaaa ccaaatcttt ttttatagaa ttcgttttaa tcctcctgaa tccaaaaact
      301 atgaaaaccg taattaaata gttaagagtt tttaaccacc ataattctct cggctatttt
      361 gaaatcagat aaattttgat cggacacata gttgaataaa cctaccgttg ttacggaagg
      421 taaatcgcat acttttcatt ctagaggaga aggtctcgtt gatcattaac tgattaagca
      481 ccatagccca gttttgaatt cgaccacctg accacttcgc gtgtaattct ttcgtgcgta
      541 aataaagtag ttttaggagt gcattctcat tagagaacgc tccttttttt gtgacttttc
      601 tgaagctgga gtggacactt tcaaccgcat tggtagtgta cataattttt cgaatggcac
      661 taccataatc aaatagttgt tcaacatgtg caaagttccg tttccagaca tctacagcac
      721 cagaataatg agaccaccga ttttgaaaac tgtcaaaagc agcatgtgca gcgtttagag
      781 aagaagcacc gtagaacttt ttcatatctc ggcagacttc cttatagtcc ttacttggaa
      841 tatagcgcaa tgcatttcga acaagatgaa caatacagcg ctgaacaatt accgatggaa
      901 agatcgcttt tgcgccttct tcgaggccag aaacaccgtc catcgaaatg aaaaagacat
      961 cttcgacacc acgtgctttc agttcatcaa atacttgcat ccagcgattt ttagattctg
     1021 tttgatttaa ccataatcct aaaatctcct tatttccttt gagatcatag ccaagaatgg
     1081 tgtatacagc atattctttg gcttcataat tttctcgtaa agtaacatac atacaatcaa
     1141 cgaatagaaa ggcataacac tttgctaggg ggcgggcttg ccattcttcc aattcaggaa
     1201 ggacagcgtc agtgatatct gaaatcattt catgggaaat atcaaagcca taaatagctt
     1261 cgacggttgc ggcaatatct cgttgactca ttcctcgtgc atacatggaa agaaccttcc
     1321 cttcgatgtc ggagacatct cgttttctct taggaattaa ctctggttca aaggaagctt
     1381 cccggtctct aggaacatca atagctactt caccaaaact ggttttaagc gttttagttc
     1441 catagccatt tcgacggtta tcgtgttcct taggctcttt agaatgggca tcataaccta
     1501 aatgattatt caattctcct tgaagcattt tttcaaaaag gggcccaaac acatctttca
     1561 aagcatcttg catgtcatcg acagattcag gttgataggc attcagaatg gattcagcta
     1621 acttttcggc atcaggattt cttttctttc tagccatcgt gtaatcacct cttgatctta
     1681 ttgtagaaaa agaaaaaccg cgaagcaacc acctgcctag gattaatggt tacttacacg
     1741 gtttatatta cactctcatc attaaaaaag tatctttcga tattactaaa caatgaaatt
     1801 atctatttga aatttaaatc tgataatata ttgagtgttc caatgagggc tttgatccta
     1861 ttatccatat ctttttcagt tttaatattg atacacatta taagaaaaag aaacttttgg
     1921 ttaatttatt tcatcccagt gcaaaatata aaaaagtaaa atagaccata ggcaatagat
     1981 taacaattaa tataatacaa gcaaatattc tattcattgt atttctgctt tcaaaaagtt
     2041 ctaatattga aagtataatt ccacaaatgc ctataataag tatgggaata agattagaaa
     2101 ataacacccc gagccaatct gtgaaaatta ctatatttac taatatccct attaacatac
     2161 ctatcatatt tattaaatca atttttttca ttagatattt ctcctttatt aaatctgaag
     2221 tacttatatt aaaataattc taaactattt acctcaaaaa gtgcaataaa ttatttctga
     2281 acaataaaat gatagtcaaa acaaaaaaat attgttaatc gttcagagcc atccgagagt
//
LOCUS       DACCKU010000184         2295 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.193, whole genome shotgun sequence.
ACCESSION   DACCKU010000184 DACCKU010000000
VERSION     DACCKU010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.193"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            94..323
                     /locus_tag="IVN13_002912"
                     /pseudo
     CDS             94..323
                     /locus_tag="IVN13_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            482..1417
                     /locus_tag="IVN13_002913"
     CDS             482..1417
                     /locus_tag="IVN13_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HAP7013337.1"
                     /translation="MSTLSCVMLLYLCIQINEIMPPHWQEKKKEFKEEIEIKSNDWIE
                     YTWLPLKRNSKRKEFWALQLWPIGKLFIYMASLMVTLIGYLVVVYGVAYYLLSEPKQI
                     AASNIFTAVMTLMCVMCFSIFSYLLVQFIRQNLTMSKLFKSLLDLLILGSMLTTLTDR
                     FEQSEKTVEGIKNFLTTIDQFINGYMYLFEVGFILTLFGCVFIKLIKRSIEKEMVIDL
                     IPTLSGYEYYVDFGKYEELIHTDIVHTSIHEVTVKGIRSTLKDGQETKTIDRRKEVYN
                     FYKVEASLRYGIGKLRLDGSNIQQNKPYVLGKVVI"
     gene            1461..1730
                     /locus_tag="IVN13_002914"
     CDS             1461..1730
                     /locus_tag="IVN13_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013338.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            2006..2281
                     /locus_tag="IVN13_002915"
     CDS             2006..2281
                     /locus_tag="IVN13_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013339.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHELMDHRKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIE"
BASE COUNT          850 a          298 c          454 g          693 t
ORIGIN      
        1 tgaaagatca agaagagagc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag
       61 aaaatattta gaagaatagg tggagtaaaa gaaatgaagc gagcgaactt ggtcggcaaa
      121 gatactttcg ctgagcagaa agtattgatt aaagcctatc tcaaagttgt aaaagctaaa
      181 gaaatatgtc aagaagtgaa ttatctaaca caacaatatc tgagttgaag aatggtagaa
      241 agagtttaga taccttaaaa ttcagatcat ttgaagctct atatatagct gctaaaaaat
      301 attttacagc tgataataaa taaaaaatta aggtgggttc tatgtgaaaa aaacgtatca
      361 aaagttttta tggattagct atatcgtcct atttatcgtt gggtttctcg tcagtgtgtc
      421 cgtgatcgtc ggaacgcttt ttcttttgtt ctctgacgaa gatctggtac ttaaggggtt
      481 aatgagtacg ttatcgtgtg tcatgttact ttatttatgt atacagatca acgaaattat
      541 gccccctcat tggcaagaaa agaagaaaga atttaaggaa gaaatcgaaa taaaatccaa
      601 tgactggatt gaatacacat ggcttcctct gaaaagaaat agcaaacgaa aggaattttg
      661 ggcattgcaa ttatggccaa taggaaaact ttttatttat atggcttcac ttatggttac
      721 tttaatcggt tatttggtgg tggtctatgg ggtggcctat tacttgttaa gtgagccaaa
      781 acagatcgcg gcgtcaaata ttttcacggc agtaatgaca ttaatgtgtg tcatgtgctt
      841 ttctattttt agttacttac tcgtccaatt tatcaggcaa aatttaacaa tgagtaaact
      901 tttcaaaagt ttattggact tacttatctt aggcagtatg ttaacgactc taacagatcg
      961 gttcgaacaa tctgaaaaaa cggtagaggg gataaagaac ttcttaacga cgattgatca
     1021 atttataaat ggatatatgt atttgtttga agtaggcttt attctcactt tgtttggttg
     1081 tgtatttata aaactcatta aaagatcaat cgaaaaagaa atggttattg atctaattcc
     1141 gacgctgagc ggctatgagt attacgttga ttttggaaag tatgaggagt tgatccatac
     1201 ggatatcgtt cacacgagta tccatgaagt aaccgttaag ggcatcagat ctactttaaa
     1261 agatggacag gagacgaaaa caatcgaccg aagaaaagag gtctacaatt tttataaagt
     1321 tgaagcaagt ctacgatatg gcattgggaa attgaggtta gatgggagta atattcaaca
     1381 aaacaaacca tatgttttag gaaaagtggt gatttagaac ttcctatgaa agatgaataa
     1441 ataaggagaa tcttttgaaa atgattcttc tttacagaat tgtgcttata gttggcacgg
     1501 agttaaaaat cttaaagaag gagcagttat tcaaagtgaa agaacatcat aatcagttac
     1561 tatgtattaa aacgggtacg atgaacattc aataccgaac agaatattta gaattaacga
     1621 atcaaactga ttttttgtcg attcgagagc aacataagca agaaaaatta atggctactt
     1681 atagccaatt gattgatgag tgggaacaat tcaaggaatc taaaacataa aattccattt
     1741 aaacacctta ttttttaaag agcataagga aacgtgtacc cactttcaaa ataaagtggg
     1801 tagataggct gtgcctatca cgtttctgaa aaaaataaat gaccccaaaa ttttcaattt
     1861 atgtgtaggt ttatgggtag caaaaatatg aatgtgggca gcaaaaagtt catttgtggg
     1921 tagtaaaatt caaatgtgtg tagcaatgtg tgtagctaca cacattgcta cacacgctat
     1981 aacatatagt gaaaaggaga tatttatgga agtaaaaata agagatattg atgaaaaaac
     2041 gattcaaaga attgaccgaa gatgtattga attaagtgaa cgcaccggga ctaaatggag
     2101 ccgaaatcga tacctaaaag ttttgattga aaatgatttt gaccatgaac ttatggatca
     2161 tagaaaagat caatttgatc ggttgttaga aaaatttgtg gatattcaaa cttataatac
     2221 gaaaatttta gaagaatata ttgcaacaaa taatcaatta atcgccttgt taattgaata
     2281 ataaaaatag tattg
//
LOCUS       DACCKU010000185         2211 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.194, whole genome shotgun sequence.
ACCESSION   DACCKU010000185 DACCKU010000000
VERSION     DACCKU010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.194"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVN13_002916"
     CDS             complement(434..1918)
                     /gene="guaB"
                     /locus_tag="IVN13_002916"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP7013340.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
BASE COUNT          589 a          479 c          404 g          739 t
ORIGIN      
        1 tttatcgaga ggttgtgaaa tcagcgaatt aacctgagta ttagagaaaa aaatggaaaa
       61 atagtccctc atattttacc gtttgttgaa ctaataccga gggtcagctg attgaacacc
      121 gtttagtgga gaggtcgtaa gcctaccccc cagctcctag ttacaaggac aaattcaaaa
      181 aacagctctt catattttat gaatgttgta cttgtgattc ggaggtggca agggaacact
      241 gtttattcaa aaagaggctg ggacagaagc gtttaactcc aagaaataag aagaaattca
      301 cgaaaattgc ttttcaaatt tttgtgaatt tcttcttatt tccgaaggag ttgcttctac
      361 ttccgccgtt tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg
      421 ctctttaatg tttttaagat tcaactgaat agtttggtgc ttcttttgtg atttgtacat
      481 catgagggtg tgattctttt aatccactgc cgctcatttg gataaattgc gcttcatcac
      541 gtaattgctg tagattggct gccccaacat aacccattcc tgaacgcaaa ccgccgatca
      601 tttggaaaat aatatctgca acacttcctt tataagcaac ccgaccttcg attccttctg
      661 gtactaattt atttgcttca ttgacactac cttggaagta acggtctttt gaaccttttt
      721 ccatcgcgcc taatgagccc atacctcggt atgttttgaa tcgacgtcct tggaagattt
      781 cgaattcacc tggtgattca tctgtccctg ccaacatact tcctagcatc actgcatgtc
      841 cgccggcagc taatgctttg acgatgtcac cagaatattt gatccctccg tctgcaataa
      901 tcgctttgcc atattgacga gcaactgatg ctgcatcata aatagctgtg agctgaggta
      961 cacctacacc ggctaccaca cgtgtcgtac aaatcgatcc tggtccgata ccaactttta
     1021 cgacatctac acctgcatca tataaagctt ttgtcgcttc agccgtcgca acgtttcctg
     1081 caatcaatgt tgcatctgca aaagttgaac ggatttcttg aatttttcgg atcacacctg
     1141 cactatgtcc atgagctgta tcgatgatga ttgcatctgc ccctgcttct aataaagcat
     1201 tcgcacgctc aaatgtatcg cttgttacac caacggctgc tgctacaagc aatcgaccat
     1261 gttcatcttt tgctgcattt ggaaattcga tcactttctc gatgtctttg atcgtgatca
     1321 atccgcttaa acgaccttcg ttatctacga ttggtaattt ttcgatttta tgtttttgca
     1381 aaatcttttc tgcatctttt agagaggtac ctactggggc agtcactaag ttgtcttttg
     1441 tcatcacatc agcaatcggc atgtgataat ctgttacaaa acgcatgtcg cggtttgtaa
     1501 ttatccctac caattttctg ttttcaagtg tttcaacgat tggcacacca ctgatacgat
     1561 atttgctcat caattcttct gcgtctgcta caagatgctg tggcgttaag aagaacggat
     1621 caatgattac gccactttca gaacgtttga cttttcttac ttcatccgct tgctggctga
     1681 tcgtcatgtt tttatggatc acacctagtc cgccttgtct tgccatagca atggccatct
     1741 tgctgtcggt aacagtatcc atacttgcac tgatgattgg aatattcaac ttaatatttt
     1801 tagctaattg tacgctcata tccacttcgt ttggtaacac gtgactttct gctggtatca
     1861 ataagacatc atcaaaagta aatccttttt tcgtgaactt tgtttcccag ttggacattt
     1921 ttagtaccac tcctctaatt tagtttatct attaaaataa ttgatttttt catttaagtc
     1981 aatgatctac ttggaatcaa gttagctttc tcatagaata ccaatataaa gcgtttacat
     2041 ctgtgtatat taaaagagat tgtaaaaaag ctgtcctgca tcaagtaata agaacagcca
     2101 atcaaaaaca gcttctcatg tttttgaatt ggttgggttt attaccgcag atgcaaactt
     2161 ttgaacaccg tttagtggag aggctgtgaa aaagctgctt agtttcgagt a
//
LOCUS       DACCKU010000186         2119 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.195, whole genome shotgun sequence.
ACCESSION   DACCKU010000186 DACCKU010000000
VERSION     DACCKU010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.195"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(501..574)
                     /locus_tag="IVN13_002917"
     tRNA            complement(501..574)
                     /locus_tag="IVN13_002917"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(538..540),aa:Ile,seq:cat)
     gene            complement(763..1782)
                     /locus_tag="IVN13_002918"
     CDS             complement(763..1782)
                     /locus_tag="IVN13_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP7013341.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1793..1999)
                     /locus_tag="IVN13_002919"
     CDS             complement(1793..1999)
                     /locus_tag="IVN13_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAP7013342.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          637 a          419 c          352 g          711 t
ORIGIN      
        1 aaaaaggtag cccggaacga attttatcac catacttgtg aaaggagata ttttttaagt
       61 tagtattaag attgtgtaat atgatgatat ctatatttta tagtatcagt gctataaaat
      121 caaaaataag tcactaatta actaccactc caattgtaag ccttttttct cacttttttt
      181 caaaaatatg gtatgccttt taatagcttc aaatataaaa gagtttaaag cgtaacacac
      241 ttatggggaa gtggcttggg gtgcgcttta aactcttctt tattatcatc tcacaattta
      301 acccaaatgt ctttctattt aaaaatcaaa gtaaaacttt tcaaatatac agaaatataa
      361 ctatgtgaaa catcctttca ttaatccata aaaggataca taaaaaagcc actcatttga
      421 gtggcaatga agaaaagctt tagcttgtat aatactcttc aaaaaattct aacacagaac
      481 gattcaaatg gctacgttaa tgtaccctgt aggactcgaa cctacgaccg gacggttatg
      541 agccgtctgc tctgaccaac tgagctaagg gtactggttg ttgccacata aagccataaa
      601 caatcaacca gtagaatgtg tggcaacaaa cctgttatcg catagcttgg agtgtgacta
      661 ttatgggtga tagtgaagat atgcgataac atcactattt tatcgaatga tttttatagt
      721 tgtcaatata gttatgtaat gctcctcaac gaggagctat ttttatcgtt taggaatatt
      781 taaataccaa cgtttgtcat ggaaatcttg cgcaccgcct ttagtgtttc cctctggatc
      841 attcgttgcc cgcatcatga catagacttt cttattaggg aagttacgca tgttaaaaga
      901 tacatgatag ccaacatttc cagaagtatt ataagcttga tttacatctg atctataaat
      961 tccatcagct cttactcgag ctaattcttt cccagtattg taatccataa tgaagatata
     1021 ctcgtattta tagttagcaa tgtgccatcc agctacatgc aaatttgcat tttcgatttc
     1081 tccaaactga tcaatgtggg cgtaattcgt tccatctgtc agtgtaggat tagctgcacc
     1141 agctcgtgtt ggatcaatga caggcttgtt ttcagaagtt gttggatttt catcggtaaa
     1201 tccatgagct aaatcatatg ctaatttttc tttacttacg cccatttcag aaagataacc
     1261 gtaaggatct gtatgatcgc cccagatgtt ttgtgttacc cataaatgcg atttgattcc
     1321 cggttggtta taaggagtgt ccaacgttaa tggaatacca tatttcattg ctgaatctct
     1381 agccaattca acgtatgcct tgtagttttt ctcaaacgtt gctttatcat gtgtgtgttg
     1441 taactcaatc tgcacaggac tgttggcatt agcatacgaa ccagcaccgt actgtacata
     1501 accaggttga ccgacttgat aaacaattcc gccgtctccc acaatataag cagtataagc
     1561 gctagtccat gaacgttgca tatactgcgc ttcattgcgt cctgttgctg tttcattagc
     1621 cgtttcatgc agtaaaatat actgattatt tgctacttgt gagctacctt catttgcgcc
     1681 caaattaaat tcattgttga tagtataggc aaatccgtta attggcaata aaaaaagagc
     1741 cgttaacagg ctcatcgcag taatagtaat tttctttttc atatctatcc tcctatttat
     1801 ctgatgaaat tttcttatac gttcgatttg atacacctaa aatactccct agaaatgctc
     1861 caaaacctgt aataatcatc accgttatat ctgtgtaccg ccagttaaca ccttttccga
     1921 caagaccgac aaaagtcgct aaagcgggga taactactat agcgatccat ttcaaaatat
     1981 caaaggttct attgttcata ctatccacct cccttcattg gttgggctac tttctttttc
     2041 aatcgcttgt tttcttctat caatatttct ctttctgaaa gcaaggcatc aatttgcccg
     2101 tccagttgtt caattttat
//
LOCUS       DACCKU010000187         2107 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.196, whole genome shotgun sequence.
ACCESSION   DACCKU010000187 DACCKU010000000
VERSION     DACCKU010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.196"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            269..664
                     /locus_tag="IVN13_002920"
     CDS             269..664
                     /locus_tag="IVN13_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013343.1"
                     /translation="MPKKRRKTIVIILTILAIGIGGKFYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIREVKVEEFKKNPVTGCYGALVTMTNNEGKSVYFDYFFSKQMNEISSY
                     GVENEEIQNEGVTTPKIRVIYSNGEAEEV"
     gene            661..915
                     /locus_tag="IVN13_002921"
     CDS             661..915
                     /locus_tag="IVN13_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013344.1"
                     /translation="MTVKDEEYRYISEHVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKDSLDGMQAMAAAPIVHSKLEKNFKNKKIPTNFRVLK"
     gene            958..1353
                     /locus_tag="IVN13_002922"
     CDS             958..1353
                     /locus_tag="IVN13_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013345.1"
                     /translation="MSRKIRKLSLAILLIVILGIGGKVYMDKREAQRQKDLLAVEKQS
                     VKVLKNTFADIKEVKIEKSARNEMTGAYRMFVTMINNHDESVYFTFGFWKERNELGDY
                     GVENKSIQKEGITVNKIRVIFSNNKEEYI"
     gene            1353..>2107
                     /locus_tag="IVN13_002923"
     CDS             1353..>2107
                     /locus_tag="IVN13_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAP7013346.1"
                     /translation="MMADKQYSVISEAVYWLDPKHRDYDHTYKENSIKKIDSYNFKIL
                     KIKDSLDGMQAMGVAPIKNGKVDLSQVVIAFAGTNLSDWKDLETDARSIMNLYGNRPG
                     ASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALYV
                     AAEKQWKNVGLNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEVR
                     ISMETAFLNLLASHSLTSWKFDENGKLIIPENE"
BASE COUNT          818 a          309 c          427 g          553 t
ORIGIN      
        1 tttgtcataa cccctttttt ttgtgacttt aaaaaaagag aaaatggcgg aaacaaacct
       61 tacgtccatc gtatacgaca caatctgtgc gtagatgcta cattttgttc actactatca
      121 aaattacttt aaaatgaaaa cgtgataaaa aagaaagtag aaaagtagaa aagtaactct
      181 aaaaaatcca aaaaaatcca ggctcgattg cgtacttatc ttaaaggaga aacaatccat
      241 catacataac ccgaaaggag acttctattt gcctaagaaa agacgaaaga ccatagtcat
      301 cattttgacc attcttgcta taggaatcgg aggaaaattt tacatggaca aaagagaagc
      361 acaaagacag aaagatttac tcgcagtaga aaaacaatcc gtcaaagttc tgaaaaatac
      421 ctttgcagat atcagggaag tgaaggtaga agaatttaaa aaaaaccctg ttactggatg
      481 ttatggtgca cttgtgacaa tgacaaataa tgaagggaaa tctgtttatt ttgattattt
      541 tttttcaaaa caaatgaatg aaattagcag ttatggtgta gaaaatgaag aaatacaaaa
      601 cgaaggggtt actactccta aaataagagt tatttattct aatggagagg cggaggaagt
      661 gtgacagtaa aagacgaaga gtatagatat ataagtgaac atgtttattg gctagatcca
      721 aaacatcctc aatatagtaa aagttacaaa gaaggcgcca ttaaaaactt tggagaaaca
      781 aaattccaaa tcctaaaaat caaagactca ttggatggca tgcaggcgat ggctgcagcg
      841 ccaatcgtcc atagcaaatt ggagaaaaat ttcaaaaata aaaaaattcc tacaaacttt
      901 cgcgtactta aataatgaag gtatcaatac ttacataaaa agaaaggagg cacgttcttg
      961 tctcggaaaa taagaaaact cagcttagcc attttattaa tcgtcatatt aggaatcgga
     1021 gggaaagtct atatggacaa aagagaagca caaagacaga aagatttact cgcagtagaa
     1081 aaacagtcag tcaaagtttt gaaaaatact tttgcggata tcaaggaagt gaagattgaa
     1141 aaatctgctc gaaatgaaat gacgggagca tatcgtatgt ttgtgacaat gataaataat
     1201 cacgatgagt ctgtttattt tacgtttgga ttttggaaag aacgaaatga attaggagat
     1261 tatggcgtag aaaataaaag tatacaaaaa gaggggatta cagtaaataa aatcagagta
     1321 attttttcta ataataagga ggaatatatt taatgatggc agacaaacaa tacagtgtta
     1381 ttagtgaggc tgtttactgg ctagatccta aacacagaga ctatgatcat acttataaag
     1441 aaaattctat taaaaaaatt gatagttata attttaaaat cctaaaaatc aaagactcat
     1501 tggacggcat gcaggcgatg ggtgtagcgc caatcaagaa cggtaaagtg gatctttctc
     1561 aagtagtgat tgcttttgca ggaacgaact taagtgattg gaaagatttg gaaacggatg
     1621 caaggagtat catgaatctc tatgggaata gaccaggagc aagtttttct gaaaaagcaa
     1681 caacaaacaa gattttatcc gttagtggat tgaaaacagc ggggcaaatc aattcagcga
     1741 ttgcatttgc taatgaagta aaaaaggaat atggagaagc tgctatttct actaccggcc
     1801 attcattagg tggattctta gcattatatg tagcagcaga gaaacaatgg aaaaatgtcg
     1861 gcttaaacgg gccggacccg tatgatctct tatcaccaga agcaaaaaaa tgggtggaag
     1921 aaaatcccgg tatgctcacc aattaccgaa atcacgccga ttttatcggt aattttggtg
     1981 gtaatggcac aggtgctgaa gttcggatca gtatggaaac agcattcttg aatctgcttg
     2041 cttcccacag tttaacctct tggaagtttg atgaaaatgg aaagctgatt attcctgaaa
     2101 atgaaaa
//
LOCUS       DACCKU010000188         2020 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.198, whole genome shotgun sequence.
ACCESSION   DACCKU010000188 DACCKU010000000
VERSION     DACCKU010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.198"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            101..571
                     /locus_tag="IVN13_002924"
     CDS             101..571
                     /locus_tag="IVN13_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP7013347.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            793..1896
                     /locus_tag="IVN13_002925"
     CDS             793..1896
                     /locus_tag="IVN13_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP7013348.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLRDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT          687 a          328 c          460 g          545 t
ORIGIN      
        1 agaatctctg aagaataaat cacgtgatcg tgacgataaa atacacttac gtcaggtgat
       61 aaaaaataca taagtagaaa actaagagga ggaacagaaa atgaaagaag ttcaattgat
      121 cagaaaaagc gaactatcag aaggtggttg caatgcatgc ggcgtagtag aagcaacaag
      181 ctatacatta aagcttggtg ccaacaaagc gataatctct gagttgacag taggcggtct
      241 tgtggacagt ctggcgcttg ctgaaggttt tattggagaa gatatttatg agatgttttc
      301 agaaatccgt cagttaaaaa aaggggaaaa ctgcatagag gttcatcatg agagcccgaa
      361 tgtgagattc aaacgtggtg ataatgagat gatcttcaat aatcatgtgt ccgatcacac
      421 cgagctatat ggaatcgtga atcaaatact aactgagctt tttggattgg gaccttatgc
      481 gttcaaggaa gaaaacggta atccaaaatt aaacgaagaa tggcaagaaa cgattgaaat
      541 acaaagaaac aacccgcact tatttcaata ggcatccaca gtatttcgta aaagggactt
      601 tgacacgact tttgtcaaaa tcccatcgtt ttttatctat ttgtgttcta aacatttctt
      661 tcagagtagt aacaactgga atgactgaaa tgtaaacaga ttatttgcct gtatcaatac
      721 ggaagagtaa tataaagtgt gtaaacgcta tcatattatt cattcaattt tttatagagg
      781 gaaggcattg aaatggaaaa aacgtatcaa gcaggtacaa atgaaggaat agtggatttt
      841 atcaatatgg aagatttaga aatagccgct tcacaagtga ttccagctgg tggttatggt
      901 tacatcagca gtggtgctgg cgacttattt acttatcaag aaaatgaacg ggcattcaat
      961 catcagctga ttattcctca tgtattaaga gatgtagaat tacccgatac aactacacat
     1021 tttgacaaag aaacgctgac tgcaccaatc atcatggcac cagttgctgc tcatggctta
     1081 gctcatgtga aggcggagaa agcttctgca aaaggtgtag cagattttgg gacgatctat
     1141 acagcaagtt cttacgcttc ttgtacatta gaagagataa gagaagcagg cggggagaaa
     1201 gcaccgcaat ggtttcaatt ttatatgagc aaagatgatg ggatcaattt ggatattctt
     1261 gaagtggcca agcgtaatgg agcaaaagca atcgtattga cagcggatgc aacagttggc
     1321 ggaaaccgtg aaacagaccg cagaaatggg tttaccttcc cactgccaat gccgatcgtt
     1381 caggcttatc aatcaggagt aggccaaacg atggatgctg tgtacaaatc ttccaaacag
     1441 aagcttagtc caaaagatgt agaatttatt gctgctcatt ctgatttgcc agtctatgtg
     1501 aaaggtgtcc agtcagaaga agatgtctat cgttcattag aatctggtgc tggcggaatc
     1561 tgggtatcta atcatggtgg ccgtcaacta gatggtggtc cagcagcgtt tgattcgttg
     1621 caatatgtgg cagaagcagt agataaacgt gtaccaatcg tgtttgacag cggtgtacga
     1681 cgcggacagc atgttttcaa agcaatcgca tcaggtgcag atcttgttgc tattggcaga
     1741 cccgttattt atggattgtc tttaggagga agtacaggag tccatcaagt gtttgatttc
     1801 tttaaaacag aattggaaat ggtcatgcaa ttagctggaa cacaaacagt agaagatatc
     1861 aaaaaaataa aattgcgtga gaatcgtttt atttaagaaa caaatgacga aaaaaatagg
     1921 agtgcggcag atgatgccgt actcctattt ttatagttca aggatgtcac taatagaata
     1981 atttgaaacg aaaaaataaa attgaaatat tttttcatac
//
LOCUS       DACCKU010000189         1991 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.199, whole genome shotgun sequence.
ACCESSION   DACCKU010000189 DACCKU010000000
VERSION     DACCKU010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.199"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(122..205)
                     /locus_tag="IVN13_002926"
     CDS             complement(122..205)
                     /locus_tag="IVN13_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP7013349.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            complement(226..337)
                     /locus_tag="IVN13_002927"
                     /pseudo
     CDS             complement(226..337)
                     /locus_tag="IVN13_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            complement(747..1541)
                     /locus_tag="IVN13_002928"
                     /note="aph(3')-IIIa"
     CDS             complement(747..1541)
                     /locus_tag="IVN13_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7013350.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            complement(1634..1885)
                     /locus_tag="IVN13_002929"
                     /pseudo
     CDS             complement(1634..1885)
                     /locus_tag="IVN13_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT          608 a          439 c          309 g          635 t
ORIGIN      
        1 atcactcctg aagtgattac atctataaac aaatacagaa gttaaacgat ttgtttgtaa
       61 ttttagttat ctgtttaaaa agtcataaga ttagtcactg gtaggaatta atctaacgta
      121 tttatttatc tgcgtaatca ctgtttttag tctgtttcaa aacagtagat gttttatcta
      181 cataacgcat ttggaatacc aacatgacga atccctcctt cttaattaca aatttttagc
      241 atctaattta acttcaattc ctattataca aaattttaag ataatgcact atcaacacac
      301 tcttaagttt gcttctaagt cttatttcca taactttagg gttaaccata cgcaagacca
      361 atcactctcg gacaatactc atgattttaa tgataaaatc catgttaaac ccatagataa
      421 gaaatacacc tgcaataacc gttacctgtt tgtgccaatt taaaaatgca ccactttttt
      481 ataattaaaa cggttgaaaa ctgtaccact aataactcac aatagagaga tgtcaccgtc
      541 aagttaaatg tacaaaataa cagcgaaatt tttaaatcta tttcttatcg atacaaattc
      601 ctcgtaggcg ctcgggaccc ctatctagcg aacttttaga aaagatataa aacatcagag
      661 tatggacagt tgcggatgta cttcagaaaa gattagatgt ctaaaaagct tgtagttaaa
      721 gctttttaga catctaaatc taggtactaa aacaattcat ccagtaaaat ataatatttt
      781 attttctccc aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct
      841 tccccgatat cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc
      901 ctgccgcttc tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg
      961 ctgtctccca ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa
     1021 aaatcataca gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc
     1081 acatcggcca gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta
     1141 ttcgtatagg gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac
     1201 agctcgataa tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca
     1261 tcggcctcac tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt
     1321 ggaacaggca gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag
     1381 gtggtccctt tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc
     1441 agcttatata ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg
     1501 atcagttttt tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt
     1561 ttctacagta tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac
     1621 tgttccttgc attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa
     1681 agttggcgta taacatagta tcgacggagc cgattttgaa accacaatta tgatagaatt
     1741 tacaagctat aaggttattg tcctgggttt caagcattag tccatgcaag tttttatgct
     1801 ttgcccattc tatagatata ttgataagcg cgctgcctat gccttgcccc ctgaaatcct
     1861 tacatacggc gatatcttct atataaaaga taacccgtgg tgctagttaa ttttttccta
     1921 gaggaaaatg tctaaacatg ttggtaaatc aacaaaaaaa cgcatatagt agtctcaaaa
     1981 ctcccaagaa a
//
LOCUS       DACCKU010000190         1984 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.200, whole genome shotgun sequence.
ACCESSION   DACCKU010000190 DACCKU010000000
VERSION     DACCKU010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.200"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..267
                     /locus_tag="IVN13_002930"
     CDS             <1..267
                     /locus_tag="IVN13_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory protein"
                     /protein_id="HAP7013351.1"
                     /translation="DYFLPDEYARNLYHGLHDQLEEKNEENMFYKIVQSFENETAKKL
                     LIQFYEEQATDRLQEYVEKLIKNEEISEMIRKRLLLEAEKAVKE"
     gene            264..>1984
                     /locus_tag="IVN13_002931"
     CDS             264..>1984
                     /locus_tag="IVN13_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013352.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTAE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTT"
BASE COUNT          693 a          285 c          420 g          586 t
ORIGIN      
        1 gattatttcc taccagatga atatgctaga aatctctatc atggactgca tgatcaatta
       61 gaagaaaaaa acgaagaaaa tatgttttat aaaatcgttc aatcttttga gaatgagacg
      121 gctaaaaaat tgttaattca attttacgaa gaacaagcaa cggatcgtct tcaagaatat
      181 gttgaaaagt tgatcaaaaa tgaagaaatt tctgaaatga ttaggaaacg gctgttattg
      241 gaagctgaaa aggcggtgaa agaatgatca ttggaggaat tttctctggc atagccgact
      301 ggtggtcagg tttattcgga gaaggggatg cagatcccaa aaccgtaacg aaagtcttac
      361 aagattatgg taattatctg cagtatcaag atgtattcag ttggatttgt aatacttttt
      421 tatggggatt gatcaaaggg ttatataatc tcaatacttt attagaaaaa acaatttatc
      481 aaagctttga tttaaaagac ttattgaacg ccgctggtgt taatgaacta tttcaaagtt
      541 tgattagtaa ggtattggct cttttttgtg tcgtcacatt aatttattta ggcttgaaat
      601 tttttatgag taaacaccca ccgaaagtca aaaatatctt ggttaattta tttatggcca
      661 tgatcttgat ttttggtggt agttctttaa tcgatgaagg gttaaatatc agtaaaaact
      721 tttatggaga cgtcactgca gcaaataaag cagataaaac gggttcacct gcttttcaaa
      781 ttattcaaaa taatgtgatt gatgtcagta cattactagc gactgacccg aataaagtag
      841 aatccttacc aacagataaa agaaatgctt taacagcgaa aaatttcaaa acagcgaata
      901 tcaatgggat tattacacct gatcaagcta agaaaatgtc tgaatcaagt gatattagta
      961 cagtacaaaa agatcggtta aaggcattac agtatcgact agagttagac gataatggga
     1021 aagaaatccc tgttcaaatt agtgatgaag gtttagcaaa atatgtgtat acgtcaggct
     1081 atcgtcgata cattagtagt ccagggatta ttttggctgg ggaatcttct ttgtcggtag
     1141 cctatttatt tattttgttt acaatcgtta cttgcattat tgagctagta tttaaaaaat
     1201 tctatttagt aattgccgcg tcaacggact ttgagacagg acaacggatg aaaagtgcga
     1261 tagaagatat ttctcaatcc ttcttgttat tggcgtttac gattttagaa ttacgaatct
     1321 atacattaat gctttcaggt atcggtgatt tgcatgcggt aggaaagatc aatggattct
     1381 tgtatgttgt tgctttaatt gtgctaacta ttgcgctatt caaaggctct caatcggtta
     1441 cgaagatttt cggtgttgat acgagtctga aaaacggcag caattctctt cttagcatgt
     1501 ttgccttagg aaatattgcg aaaaatgtgg gtagcggagc taaaaacctt gcaagtgctg
     1561 gtaagagtgg tttaagccat ttaaatgata ttcgtaagaa tggatttggc catcgaaaag
     1621 atattaccca tgataacggg gaagaacaat cagacgataa agctacaact gtacctaata
     1681 gtaagaagac agtttccgaa atgttcggga aagcgaaaaa tggtgtttct aaagccgcag
     1741 aaggtcttgg ttacgtcaat gaacgtgggt taaaaggaac agctgaagat cttacagaaa
     1801 aagccaaaga caccttagaa gagaaattag acggcacgag agctaaagaa tttgctgaag
     1861 ctgtgaagaa tccatccgaa gcactagaaa aacaaaaaga aaaagcttca gcagtcaaag
     1921 aaggactaaa agatgcgtat ggtgaaggac aagtcaatgc agcagtgaag gcaaatacaa
     1981 caag
//
LOCUS       DACCKU010000191         1970 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.202, whole genome shotgun sequence.
ACCESSION   DACCKU010000191 DACCKU010000000
VERSION     DACCKU010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.202"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..85
                     /locus_tag="IVN13_002932"
     CDS             <1..85
                     /locus_tag="IVN13_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP7013353.1"
                     /translation="QIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            102..1187
                     /gene="uxuA"
                     /locus_tag="IVN13_002933"
     CDS             102..1187
                     /gene="uxuA"
                     /locus_tag="IVN13_002933"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP7013354.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            1180..1893
                     /locus_tag="IVN13_002934"
     CDS             1180..1893
                     /locus_tag="IVN13_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP7013355.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT          692 a          288 c          460 g          530 t
ORIGIN      
        1 acaaattgtc aaacaaggaa aagataattt aactgtttgg catgcttcag cggaagaaaa
       61 aaaattagat gaggacgatt tttagaagga aggctgaaaa gatgaagtgg ggatttagat
      121 ggtatggtga aaaaggggat tcgatcccat taaaatatgt tcgccaaata ccgggaataa
      181 atgggataat tggaactttg ctgactaaaa tgcctggaga tgtttgggag atttcagaaa
      241 ttgaggagtt gaaagcatct gttgagagcc aaggactgga actgcttgga attgaaagtg
      301 tagcgatcca cgatgcaatc aaggcaggga cagatgaacg agattactat attgataact
      361 atatacgaac aatcaaaaac ttgtctcgat gtggggttcg attgatttgt tatagcttca
      421 aaccaatttt tggatgggcg aaaactgatt tgtttaatga aaacaacgat ggaagctatt
      481 ctcttttata cgatcagaaa atcgtcgatc agatggagcc aggagaaatg tatcaattga
      541 tacacagcca gtcaaaggga ttcaaattgc cgggttggga agaagaacgg ttgaaaaaat
      601 tcgctcagct ggaaaaactg tatgaaggaa tgaccgaaga agaattattt gaaaatctga
      661 cttatttttt agaaaaagtg attccagtgt gcgaaaaagc agatgtaaaa atggctatcc
      721 atccagatga tccgccatgg gaactttttg gttttcctag agtgacgaaa aatcttggcg
      781 acttgaagaa aattctaaat gcagtagatt ctccatataa tggactaacg ctgtgcacag
      841 gatctttagg ggcaaatccg gaaaacgatt tagttgaaat catccatgag gtaggttcgc
      901 gcatccattt tgtccacttc agaaacgtgg agtttttagg agaaagaaag ttcaaagaaa
      961 cggcacatcc ctctacagaa ggctcccttg atatgtatca tattatgaaa gcgttggtgc
     1021 aagaagggtt cgacggtcct atacgccctg atcatggtag aaccgtgtgg ggtgaagtgg
     1081 ctatgcctgg atatggcctg tatgatcgtg caatgggact cacttacatg caaggtctat
     1141 atgaggcgat aagtaaggaa aaaccactgg gagagcatta tgaataaaat caagctcttc
     1201 atctttgata tggatggcct tttattagaa actggacgtt tagcttatcg tgcttatgta
     1261 aaatcagcac aaaagtatga ttatgaaatg cgtaaggaag tctattattt actaactggc
     1321 caaacagaaa tggcgataag agaacaaatg ggcattttgt acggagaaga tgttccatat
     1381 atcaaatggc gagaagcaat caatcaatat aaagaaaaaa tcgtgaaaga agataagaga
     1441 gtatatacga aaaaaggagc agaagaaatt ttatcttttg caaaagaaag aggaatccat
     1501 acaatcgtgg cttcgtctaa tacacgggta aaagtagaaa tgtatttaaa aatggaaaat
     1561 ctatatgcct tgtttgatca aatcatttca ggggatgatg taaaaaaagg aaagccggaa
     1621 ccagagatct ttttgaaagc ttgttcaaaa atgaatattc ctccaagtga agcacttgta
     1681 tttgaagact cgattgcagg gatcgaagca gcaaggcgag cgggcatttt gtcttttctc
     1741 atcaaagatc atttgatagg gttgcccgat cataatggga agcataagtt gaaagtagat
     1801 gtaagtttat atgaatccaa taaagcagac tatgaatttt atgacctgca tcaagcaaaa
     1861 cgatttttag aaagtaaagg gttgtatctc taataaagag gactattttc tagtcctctt
     1921 tatttattgt cctgcacgac tgagggaaca gatgaaaaag aagtaatttt
//
LOCUS       DACCKU010000192         1910 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.203, whole genome shotgun sequence.
ACCESSION   DACCKU010000192 DACCKU010000000
VERSION     DACCKU010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.203"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            492..710
                     /locus_tag="IVN13_002935"
                     /pseudo
     CDS             492..710
                     /locus_tag="IVN13_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            970..1224
                     /locus_tag="IVN13_002936"
     CDS             970..1224
                     /locus_tag="IVN13_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP7013356.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            1225..1542
                     /locus_tag="IVN13_002937"
     CDS             1225..1542
                     /locus_tag="IVN13_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP7013357.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          661 a          300 c          355 g          594 t
ORIGIN      
        1 cattatatca ccaaacccca tcagcgtgca ggctgatggg gtttttttac gtttctatat
       61 tatagaatta acgggtaggc ttactgtctt ttttggcaat tttgcaatca agccatttgc
      121 agaggtaact gcacactaca tctgcaaatg gcttgattgc aaaaacaaaa tcaaaaaggt
      181 atttcattat atctcctcct ccccttgaca gattaggccg gggtagcaga cagcaaaact
      241 attataacat aactgaaata cctaaatgac atatacagat atgataattt atgtattgat
      301 ttttctaaca aaagcacata ttttaatata tactacctat tttaggtagt atatattttt
      361 agatacaata tcgatcagtt ttgaggaaat gaaattctag atattttttg ctaaatagag
      421 ttactttcaa tattttttaa cgtaattagg cagaagtcac tcatcctcta taggtttgtg
      481 atgaaattgc cattgatcca tttaagtaag agcaaaaaac tggaggttta tcaggtaatt
      541 ggtctaatgg atttaagtat gcggtcaccg tgtatagagt tgcttatgag attcaagaaa
      601 ataaagttat tccagtattg ctatatggaa cacataacaa tttttatgaa ttagccgaga
      661 acactcgcaa ctttattcgt gtagatgaat cggcttcggt tgagttataa ctttagctat
      721 atcaatcaac cgaccgacat atgttaaatc tatatataat aaaaatacct acttaaatgg
      781 atgttaacgg aagtgtatgg tgagtagttc actcgtattt tgatatcgtt tgtatatgtt
      841 ttcagcgttc taagtggctt attttgtcca ataataatat ggagtggaat cccttaagaa
      901 acaccgttgg caattgttgt tataacatgt tataatttat tcatcaaaaa ctaaaaggag
      961 tgtgataaca tgggaaaaga actaaaggtt agaaagatcg gcaatagcgt gggagtgatc
     1021 cttccaagct ctcttggatt aaaaagcgga gacactatcc aagccaaaca agaaggcaac
     1081 ttattcatat tagatactac tcaaatagca aaagaacacg atcgcaaatt gattgaagaa
     1141 agtttccaag actttgagaa aggtttaaca gtttcagaaa ttgaaatggt aaaagctttc
     1201 ggaaaatacg gctggagtga atagatgaaa gagtacacgc tagtttattc taaaagtttc
     1261 cagaccagcc ttgaaacgat aatagaagaa tgggaaaatg agcttgctct gtctccagcc
     1321 agcataaaaa gatttgtagg tgcgatccag aaggctttag aactaaccaa aaattttcca
     1381 actatgcatg aggaagtttc cgaaatctat ggaatggata tcccaacata tcggatcgtg
     1441 atcggcaaaa gctatgcctt attttatcgg atcgaccatg aaaaaaaaga gatacttgta
     1501 ggacgtattt tccaatctaa acaaatgaaa ctaggctttt aacaataatt aggcaaaagt
     1561 cgctcatcct ctacaggtgg gtgattaatg ccgctcgata tggttgttaa ccgtcggtta
     1621 ctcgaagatt gatatataag acttagtacc tttttatttt gctccatgtt atggccgaag
     1681 gtctaaagat acaatcatta gtctagagaa aaattaagaa ccgtaggaac tacggggatc
     1741 gcttggtgaa taagagacac tgctattagt gaaaaaataa gatatcaagt acgctctatt
     1801 cccaaaaatt tcgtgacttt actcatgaga ggttcaagta gtatattttt aatcaaaatt
     1861 gacgtaactt ttaataaata ctacctaaat aaggtagtat ttatttttgt
//
LOCUS       DACCKU010000193         1909 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.204, whole genome shotgun sequence.
ACCESSION   DACCKU010000193 DACCKU010000000
VERSION     DACCKU010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.204"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(325..1188)
                     /locus_tag="IVN13_002938"
     CDS             complement(325..1188)
                     /locus_tag="IVN13_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP7013358.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(1190..1462)
                     /locus_tag="IVN13_002939"
     CDS             complement(1190..1462)
                     /locus_tag="IVN13_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP7013359.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(1479..1694)
                     /locus_tag="IVN13_002940"
     CDS             complement(1479..1694)
                     /locus_tag="IVN13_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP7013360.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(1786..>1909)
                     /locus_tag="IVN13_002941"
     CDS             complement(1786..>1909)
                     /locus_tag="IVN13_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP7013361.1"
                     /translation="TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT          514 a          335 c          303 g          757 t
ORIGIN      
        1 ttacttatga gcaagtattg tctattttta atagttatct attatttaac gggaggaaat
       61 aattctatga gtcgcttttg taaatttgga aagttacacg ttactaaagg gaatgtagat
      121 aaattattag gtatagggca cctctaataa ctaccaatta attcacctca aaatgaaatt
      181 agaaaaaaac agcgaaattt ttaaatctat ttcttatcga tacaaattcc tcgtaggcgc
      241 tcgggacccc aacatgagga aatgttttta taaaaaaaga gtagttaccg ttttttggta
      301 actactcttt tcctttttta aggtttaaat acctggaagt ttaggtgttt tgggtattgg
      361 tggtgtaggt ggctgcaagc tttccaattt ttgttgaatt gctttaaatt caggtgtctc
      421 ttggtgttta tttagaacca ttttttgctc tattcgttct aaagtcggtt gaatttcttt
      481 ccctgatact ttacgattca attctttttc taaggtttct tttggactaa tagaaggtgt
      541 ttccaaactt gaatagagtt ttactccttc tctgttatat agccttatat cgctaaataa
      601 gcccgtttta tgaagggttt ctaaattggt cggtaagttt ttgacaacaa tatcatgcgc
      661 ttgttttggt gttgccctgg ctgtcattgg atcatctgca tacatggttt catatcgttc
      721 aattgttcct aaatatgagt tgattttagg tactgccatg acatacattt ttgtttcata
      781 acctttggct tgaagcattg ttgcggtttg aataggaacg tctgttgttc gtcctgtacc
      841 ttcgatcacc aaattatacc cttgatcgct caaacggctt atgatcgctt ctgtcatgcg
      901 attagaataa ggggtaacgt gttttactac gtctttttca taaagtttca ctagttcatc
      961 aaaattaggg tgctgttgtt taaaggtatc attatcaatg acaataacat tcccttgtgt
     1021 ttcttcaaaa attgctgatc gcaaactggt tttccctgac cctggttgcc caccaagtaa
     1081 aaaagcggtt ggcgattcaa ccgctttttt tccttgaatc aattcttcta aattatcatt
     1141 taagcgattc tcaaattgtt tgtcagtaaa attgactata tttgccatat taagccactt
     1201 tctctttatt caaaacttgt tttgagtaac gattcattga ccgccaatac tcatgaacgg
     1261 cttgtaattc ttctaaagaa taatcaaaaa gatttacacg ttttgcaagc tttaattggt
     1321 ttaataaatt gacttccaat aattgagagt catttttatt taattcatgg ttcaaaacat
     1381 agtttaatac tcgattataa acgcttgccg ataattcgtt aatgatctct atttcaaatg
     1441 ttttttcata cgtaactgcc atttcatttt cactcctttt aaagtttgtc aggtaaatat
     1501 ttccgtatat attcttctaa ggcttgatcc ataacttctt ttacgtttcc gccattcttt
     1561 gctgtttcga tttttatgat atggtgcaag tcagcacgaa cacgaaccgt cttatctccc
     1621 attatatctt tttttgcact gattggtgta tcatttcgtt ttgctttttg tgcgcctaaa
     1681 tttcccacaa tcactcactt ctttctattt cttcttactc ttattttatc atcaacaatc
     1741 acaaatcaca agtgatttgt gattaatcac ttgtgatttg tgattctatt ctttttcgtt
     1801 ttctaattga ataattcgct caagcatttc aaaaaatacg ttcttataca tggataaaac
     1861 ttttttgtca tagcctttat gctctgtaat gccgttttta gaccaggta
//
LOCUS       DACCKU010000194         1888 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.205, whole genome shotgun sequence.
ACCESSION   DACCKU010000194 DACCKU010000000
VERSION     DACCKU010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.205"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            6..398
                     /locus_tag="IVN13_002942"
     CDS             6..398
                     /locus_tag="IVN13_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7013362.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            399..1838
                     /locus_tag="IVN13_002943"
                     /note="aph(2'')-Ia"
     CDS             399..1838
                     /locus_tag="IVN13_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7013363.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          787 a          138 c          322 g          641 t
ORIGIN      
        1 agtttatgga agaagtttta attgatgata atatggtttt tgatattgat aatttaaaag
       61 gatttcttaa tgataccagt tcatttgggt ttatagctaa agaaaataat aaaattatag
      121 gatttgcata ttgctataca cttttaagac ctgatggaaa aacaatgttt tatttacact
      181 caataggaat gttacctaac tatcaagaca aaggttatgg ttcaaaatta ttatctttta
      241 ttaaggaata ttctaaagag attggttgtt ctgaaatgtt tttaataact gataaaggta
      301 atcctagagc ttgccatgta tatgaaaaat taggtggtaa aaatgattat aaagatgaaa
      361 tagtatatgt atatgattat gaaaaaggtg ataaataaat gaatatagtt gaaaatgaaa
      421 tatgtataag aactttaata gatgatgatt ttcctttgat gttaaaatgg ttaactgatg
      481 aaagagtatt agaattttat ggtggtagag ataaaaaata tacattagaa tcattaaaaa
      541 aacattatac agagccttgg gaagatgaag tttttagagt aattattgaa tataacaatg
      601 ttcctattgg atatggacaa atatataaaa tgtatgatga gttatatact gattatcatt
      661 atccaaaaac tgatgagata gtctatggta tggatcaatt tataggagag ccaaattatt
      721 ggagtaaagg aattggtaca agatatatta aattgatttt tgaatttttg aaaaaagaaa
      781 gaaatgctaa tgcagttatt ttagaccctc ataaaaataa tccaagagca ataagggcat
      841 accaaaaatc tggttttaga attattgaag atttgccaga acatgaatta cacgagggca
      901 aaaaagaaga ttgttattta atggaatata gatatgatga taatgccaca aatgttaagg
      961 caatgaaata tttaattgag cattactttg ataatttcaa agtagatagt attgaaataa
     1021 tcggtagtgg ttatgatagt gtggcatatt tagttaataa tgaatacatt tttaaaacaa
     1081 aatttagtac taataagaaa aaaggttatg caaaagaaaa agcaatatat aattttttaa
     1141 atacaaattt agaaactaat gtaaaaattc ctaatattga atattcgtat attagtgatg
     1201 aattatctat actaggttat aaagaaatta aaggaacttt tttaacacca gaaatttatt
     1261 ctactatgtc agaagaagaa caaaatttgt taaaacgaga tattgccagt tttttaagac
     1321 aaatgcacgg tttagattat acagatatta gtgaatgtac tattgataat aaacaaaatg
     1381 tattagaaga gtatatattg ttgcgtgaaa ctatttataa tgatttaact gatatagaaa
     1441 aagattatat agaaagtttt atggaaagac taaatgcaac aacagttttt gagggtaaaa
     1501 agtgtttatg ccataatgat tttagttgta atcatctatt gttagatggc aataatagat
     1561 taactggaat aattgatttt ggagattctg gaattataga tgaatattgt gattttatat
     1621 acttacttga agatagtgaa gaagaaatag gaacaaattt tggagaagat atattaagaa
     1681 tgtatggaaa tatagatatt gagaaagcaa aagaatatca agatatagtt gaagaatatt
     1741 atcctattga aactattgtt tatggaatta aaaatattaa acaggaattt atcgaaaatg
     1801 gtagaaaaga aatttataaa aggacttata aagattgatt atataatata tgaaaagcta
     1861 ttataaaaga cattagtatt aaatagtt
//
LOCUS       DACCKU010000195         1832 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.206, whole genome shotgun sequence.
ACCESSION   DACCKU010000195 DACCKU010000000
VERSION     DACCKU010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.206"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            32..862
                     /locus_tag="IVN13_002944"
     CDS             32..862
                     /locus_tag="IVN13_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP7013364.1"
                     /translation="MSLLIYTHVLSTFMSVIVIGFLFIGSLFSKRKEKKEMSDLILNM
                     IKAVGWTILLTAYVWYPMLQQVLHQKINRPFRTNLQERALNISDSLIGAVNNDLVTFT
                     MGTIGVVSLILPLFFLKKFTNKEKIIYFCAVTTWFLSTNLFPWEILQKTPIQIIQFPW
                     RILGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAATEANYSHKIQSYK
                     GRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDRWRMHLIKHIIPK
                     "
BASE COUNT          654 a          250 c          288 g          640 t
ORIGIN      
        1 aaagaactgt gtgattcctt tagctatcag tatgagtttg ctaatctata cccatgttct
       61 ctccactttt atgagtgtga ttgttattgg cttcttgttt atcggctctc ttttctctaa
      121 gcgaaaagag aaaaaagaaa tgtcggatct gatattaaat atgataaaag ctgttggttg
      181 gaccattctt ttaacggctt acgtttggta tccgatgtta caacaagtcc tacatcaaaa
      241 aattaatcgt ccttttcgca caaatcttca agaaagggca ttgaatattt ctgatagttt
      301 aatcggtgca gtaaataatg atttagttac gtttacaatg ggaacaattg gtgtggtttc
      361 attgattctt ccacttttct ttttaaaaaa atttaccaat aaagaaaaga ttatttattt
      421 ttgtgcagtg accacttggt ttttatcaac gaatcttttt ccatgggaaa ttttacaaaa
      481 aacacctatt caaatcattc aatttccgtg gagaatcttg ggttttcaag tgctttttgg
      541 ttcattaatc ttaaccatag tgtttttaaa gtggaaaaca agcaacaaaa aaagtatgtg
      601 gagccttgta ggaatagttc ttattatttt tactgtgact gcagcaacag aagcaaatta
      661 ttcgcataaa attcagtcgt ataaaggtcg tttagttatg accaaaaaag atgttacacg
      721 ttatacatcc tcaagaactg gaggcctata tgactatgca ccattagatg cattgaaata
      781 taaagatcat ttaaagaaac atgaagtaaa agttggtgat cgatggagaa tgcatctcat
      841 aaagcatata attccaaaat agtctataca gtagaagatg ccgatgagca aaatatcaca
      901 ttacctgtat tcaattattg gggaacagtt gtgaaagtaa atgggaaaaa gagcactata
      961 gaaaatgaca atggtttagt gtctattaaa ggtacgggaa aaagaactat agttgaggtt
     1021 tcatctacgt atccaactac gatgattcta tctttattaa ttagttcaat aacttttata
     1081 ttcttattta cacgttattt aataagatgc tgcggttaaa tgaaaataga ttagatagat
     1141 attataaata tgttgattct attttcatag caatggagaa aatacattag caatatataa
     1201 aagaaggtat caagagtcac agtgaattta ttccgaaaag tgaaaagagc aaatataaaa
     1261 tttaataaaa atatagaact aaataggaaa acgtagacgt aattttctac gttttccttt
     1321 tcatatatat taaatattac ggaaaattat ataaactaat ttagtgtttt gtttgtaatt
     1381 cctaattttg ctccactttt agataatttt taggtctatt taactcatca gacattctat
     1441 gctcattttt ctccaaattt gtttcaactc tttcgtactt aattttgcgt atttgaaaat
     1501 tcttctttta gagtactttt attacgatac tagtatgata tatatctata gcaaacagtt
     1561 aaaatcttcc taaaataagc gaatagcatt cctaaaacct ataaaatttt tagatgaagc
     1621 gacaagtgga caattaataa actacttgaa catgaaatta tggatcacct ttcaaattta
     1681 gaaaatggaa ttcttatact tattgcgcaa catttattga ttgcaaaatt atgtgacaaa
     1741 aagaactagt agaagaagga atgatggctt gataataaag tactacctat taaaggtagt
     1801 actttattta atttagtata aattaacata ct
//
LOCUS       DACCKU010000196         1774 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.207, whole genome shotgun sequence.
ACCESSION   DACCKU010000196 DACCKU010000000
VERSION     DACCKU010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.207"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            197..424
                     /locus_tag="IVN13_002945"
     CDS             197..424
                     /locus_tag="IVN13_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP7013365.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="IVN13_002946"
     CDS             547..873
                     /locus_tag="IVN13_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP7013366.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="IVN13_002947"
     CDS             941..1654
                     /locus_tag="IVN13_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP7013367.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          374 g          441 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt gttt
//
LOCUS       DACCKU010000197         1647 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.208, whole genome shotgun sequence.
ACCESSION   DACCKU010000197 DACCKU010000000
VERSION     DACCKU010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.208"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            342..579
                     /locus_tag="IVN13_002948"
                     /pseudo
     CDS             342..579
                     /locus_tag="IVN13_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(723..1499)
                     /locus_tag="IVN13_002949"
     CDS             complement(723..1499)
                     /locus_tag="IVN13_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP7013368.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
BASE COUNT          527 a          286 c          251 g          583 t
ORIGIN      
        1 ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt cgtgagcctg
       61 tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca gcttctcatc tttgatgaat
      121 atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc tattttttct
      181 gtaaccaaaa aacgatggaa cgttgttttg attgccctta atgaggacag tcggaacagg
      241 tttgtcattt ttatatttaa atggcacaaa tttttctaat tatttgtcat ttagcgctga
      301 aaatttttgc tatttatcta aaaaagtgat aactacttaa gatgaagcag aatatttacg
      361 ccatacggca tacaaccgcg aaaaatataa aaaaaagaaa acgattgaac gcgtttttgc
      421 agtcgcaaag gaaaagcatg gaatgcgcta catcaagtat cgagacttgg aaaacaatca
      481 ggtgtatacg ctgattgttt ttgctgcaat gaatctgaaa aagttgtcca cttggcagtt
      541 ggaacatatg tcttcatatc ttttattata caaaaatagc aaaagcacca gtctacactc
      601 ttagtaaaga ggaaactgat ccttttgtct acattcttaa agtacttaaa ctaagtactt
      661 tattttactc tttaggattg atacagtagc atctatccct ttattaataa tcttttattt
      721 ttttaaataa agatgttgtc tccatcttca ggcactaaaa ctttttcgga aattcctttt
      781 tctttattga aagactttaa ttcttctctt gacagtcccc aatggtttac agcttccata
      841 tgaacggcaa taattgtgga atctggtatt gctttatgca attcatataa atcgtctttc
      901 cccataataa gagattctcc aactaaaaaa tgattatctc cagcatttgc tactatgatt
      961 tctggattat actgatcaat agtttgttta acagcttcat accaaactgt atctcctgta
     1021 aagtatagtg tcttctcagt cgaatgtttg aaaacaatac cgcaaacatt acctacataa
     1081 ttcaagattt ctcctctgcc atgtttgcct tttgttttaa ccaattgtat agattcaaaa
     1141 actgtgtctt ctcgtagtac ctccacattt ttaaaaccat atagtcgaat ttcattcgca
     1201 tcattttcat cttgcacaaa taattttatt tctttaggta ggacctcttt cgctatatcg
     1261 tcccaatgat ctagatgtaa atgtgttaca ataacagcat caataccgtt gataatatct
     1321 tcgactggca ttggcaaatc aactagagga ttcatttgat cttttcttgg tgatggaaaa
     1381 ggacctttcg aaataaatgg tggaagtgtt cctttttccg ctaacattgg atcaattaaa
     1441 aattttttac ctccatagtc taagatagct gttgcattac gaatgtgatg tatattcata
     1501 aacgttgtct cctttttgtt gatatgaaat atttttagta taattcaatt ccatacaata
     1561 aataaaccga acaaaaaaag tcttttacta tttttactta atttatgaaa ggttgtttac
     1621 catgttagac caaacagata taataaa
//
LOCUS       DACCKU010000198         1599 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.209, whole genome shotgun sequence.
ACCESSION   DACCKU010000198 DACCKU010000000
VERSION     DACCKU010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.209"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            76..1395
                     /locus_tag="IVN13_002950"
     CDS             76..1395
                     /locus_tag="IVN13_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAP7013369.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            1367..1564
                     /locus_tag="IVN13_002951"
     CDS             1367..1564
                     /locus_tag="IVN13_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013370.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
BASE COUNT          573 a          244 c          360 g          422 t
ORIGIN      
        1 gttaagggct aaaaaggctg cactaccagt cttttagccc ttttcatttt gtttttgaac
       61 aagtaaaata ttactatgaa agcgaatatt ctcgaagcaa tcttttttga cgaacaccat
      121 aattgggata actttgttga gaaatacggg cgaaagattc ggctagtcgt caaacgggaa
      181 gtaaacaagt ttagatattg tggagatatt caaaaaggat accgactgtt tgtctgcgag
      241 ggatgtcatc aaacaaagtt tgttcccttg aaatgtcgtg gaaagttctg tccaacgtgt
      301 gcaacaggtg aaagccaaag gtggtcagcg gttgtggcga atgatatgtt taaagtgaca
      361 catcggcacg taatctttac gatcgatgag gggttacggg aaatctttat gatggaaaac
      421 tatcgagatt ccttactgaa aggactaatg gatgaagccg caaaaattat tcttgcctat
      481 tttgagaaaa gaaaggtgaa ggcaggtata gtagctacaa tccacacctt tggatcaaaa
      541 ctagaattta atccgcatgt tcatatggtt gttactatgg gtggcttaac aagagatgga
      601 agttgggaag aatacgatta tttacctttt tctatgttaa gaaaatactg gaaaaatgct
      661 gtattgaaac taattagacg aacgttaggt gagtgggaca agcaaagagt tcaatcaaga
      721 ttacaagctg cgtacaagaa caatggagaa gggttttacg taaatgctcc taaaaggagc
      781 aggacaaatc taaagtcact actccaatac atcagtcgtt atatgaagcg tggcccgatc
      841 gcattaagcc gaatcttaat gtatgatggc gacactgtca tgttcaacta catggacaaa
      901 cgaacgaata ctaaagagac gatgaccatg agtatagacg aattcatagc tgcactgatt
      961 cgccatattc aaaataagaa tttcaaaacg attcgtcgct atggaatcta tagtcgtaga
     1021 atcaaaacat taatgaaaaa ggtcatgaag gagtatcaga aacgagtaaa aaagctgttt
     1081 atcaatctga atgaggcctt aagaccaaaa tcctgggaag aaagaataac cgaagaattt
     1141 ggtgaaaatc ccttagaatg ttcttgttgc ggtgagtact atgaattcgt tgggatgagt
     1201 gtgcgcaaaa atggacggtt agttattaaa tatgcgaaag ataaccatgc aaagcggttt
     1261 atgcgagagg agaatcgaaa aattgaccaa aaagaattca ggcaacagta tgaagaagaa
     1321 aaagaaaaaa cttttaaagc aattcgctgg gattgggaac gccaacgtga aatttatatg
     1381 tctaagttgt ggtgaaaaag aatcaatccc aatggatgta gtaaaatttt tagatgatgc
     1441 agatattgca aatgatccga acaatcctcc ccaatttacc tgtgaaaaat gtgggaagga
     1501 gatgtatcca gaatattata gaaacgccct tggaatagag tttaagatca gtgatgttca
     1561 ataagaaggt taatcataca aaaagtccac cggagaatt
//
LOCUS       DACCKU010000199         1590 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.210, whole genome shotgun sequence.
ACCESSION   DACCKU010000199 DACCKU010000000
VERSION     DACCKU010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.210"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            90..941
                     /locus_tag="IVN13_002952"
     CDS             90..941
                     /locus_tag="IVN13_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013371.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            962..1174
                     /locus_tag="IVN13_002953"
     CDS             962..1174
                     /locus_tag="IVN13_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013372.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIE
                     YRTEYLELTNQTDFLLIRKQQEQEKN"
BASE COUNT          595 a          207 c          320 g          468 t
ORIGIN      
        1 tacttaaaaa attggttcat tgagcaacct atcgctgcta aagtagcaaa ggaaaacgtt
       61 aaaggaaaga atttagggaa gaaatcgaaa tgaaatcgga cgactggatt gaacacacct
      121 ggcttcctct gaaaagaaat agcaaacgaa agaaattttg ggtattgcaa ttatggccat
      181 taggaaaact ttgtagttac ggggcacgac ttattcttga tttaaccgtt tactcgattg
      241 gagtatttgg aacggcttat tacttgataa atgatcccaa taggattgca gcatcagaca
      301 tttttacagt gctaatgacg ttgatttttg tcatgctatt gtctattttt actcaattag
      361 tcatgcaatt taccaggcaa aaattaacag caggaaaact tttcaaaagc ctattcgatt
      421 taattgttgt aaatgcaatt ctagcgactt taataaaaca aattgaacaa tctggaagaa
      481 caacaaaagg agtgaaggat tttttattga cgtttattca ctttgtaaat ggacacatgt
      541 atttgtttga agtgggtatt attctctttt ttgtcgggta tgtatccacg aaacttatta
      601 aacgttcaat tgaaaaagaa attgaaattg acctgattcc gacacttctt ggttatgagt
      661 acaatgttga ttttggaaga tatgacgaat tgatcaatac ggatatcgtt cacacgagta
      721 tccatgaatc tactgttaag ggcatcagat ctattttaaa aaatggccag gaaatgaaag
      781 caatagatcg aagaaaagag atctacaatt tttatgaagt cgaagcaagc ctacgatatg
      841 gcatcggaaa attgtggtta gatggaagta atattcgtca aaacaaacca tacatttttt
      901 ggaacagtag tgatttagaa cttccaatga aagataaata attatggaga atcgtttgaa
      961 aatgattcct ctatacagga ttgtgcttat agttggcatg gagttaaaaa ccttaaagaa
     1021 ggagcagtta ttcaaagtga aagaacgtca taatcagtta ctatgtagta aaacgggtac
     1081 gatgaacatt gaataccgaa cagaatattt agaattaacg aatcaaactg attttttgtt
     1141 gattcgaaag caacaagagc aagaaaaaaa ttaatggcta cttatagcca attgattgat
     1201 gaatgggaac aattcaagga atctaaaatt ccagaacgtc tctaaaagcc tttatttgta
     1261 aagagcataa ggaaacgtgg gaacactttt taaaaaaagt gttccaatag gctgtgccta
     1321 ttacgtttct caaaaataaa tgtgggaaca aaatatttaa aaataggata gtgaaaggat
     1381 agtgaaggat agtaaaagga gggtagtagg atagcaaaaa ggataggctc cttttcgcta
     1441 tcctttttaa attggaggaa ttaatatgga agtaagaata agaaatgtgg atgagaaaat
     1501 cgtaagagaa attgatcgaa gatgtgacga acggttctgt tgcaaagttt taaatctact
     1561 atcaaataag gtagaataat agaaaaagat
//
LOCUS       DACCKU010000200         1585 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.211, whole genome shotgun sequence.
ACCESSION   DACCKU010000200 DACCKU010000000
VERSION     DACCKU010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.211"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..445)
                     /locus_tag="IVN13_002954"
     CDS             complement(<1..445)
                     /locus_tag="IVN13_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HAP7013373.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISG"
     gene            499..960
                     /locus_tag="IVN13_002955"
     CDS             499..960
                     /locus_tag="IVN13_002955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013374.1"
                     /translation="MKKLLYILLFSTFAIVALLLLFEHLKRVNLDGTLLEEQIAILNT
                     PKLLIVFFFMCMLSALIFMFYISTFWVYKEIKNKEFTDKDMIFMGFLVKILFIFSIVI
                     TLLFCLNETQFSVATTLISFIAIFSFIFSDNQKKSINGFLQKIKKKINGEP"
     gene            complement(1182..1481)
                     /locus_tag="IVN13_002956"
     CDS             complement(1182..1481)
                     /locus_tag="IVN13_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013375.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKGLSPSDVLKEAS"
BASE COUNT          456 a          299 c          233 g          597 t
ORIGIN      
        1 gtccgctaat tcgcatagta tctttaagca tggaaacatc ttttttaaca tctttcattt
       61 cttctaattg atagatcatc atgtcttcaa ttgtttgagg aacagtattc ttccgaataa
      121 catcttccat ttcgttgaaa gcttcaataa attttagttt gaaatttatt gctttacttc
      181 cagtgaatcc catagctagc aaggaaaatc cgtctctatt catgaaataa actctccgac
      241 ttcttccgta tgagtctggc tcgttccctt ccacaaacat ctgtccaaaa ttggacccat
      301 cttcaacatt ggccgaattt tcgaccgatc tttttattga ttcgattgct tctagcacat
      361 gcttgtgttt cttttcaaaa ctgtctgcga cttgtaagct cgtagttaca gcttctttat
      421 ttttcaaaat tactaattct tgcatttttt cttctctcct tttgatataa tttaggtaaa
      481 aaatggtggt gaaaatattt gaaaaaattg ctatatattt tacttttttc aacctttgca
      541 attgttgcat tattgttgct tttcgaacat cttaaaagag taaacttgga tggaactttg
      601 ttagaagagc aaattgcgat tctaaatact cccaaattac taattgtatt cttttttatg
      661 tgtatgttat ccgctctgat atttatgttc tatatatcaa ctttttgggt atacaaggag
      721 attaaaaata aagagtttac tgataaagat atgattttca tgggtttttt agtcaaaatt
      781 ctttttatat tttccatagt aatcacttta ttattttgtt taaatgaaac acaattttct
      841 gtagcgacca ctttgatttc atttattgct attttttcct ttatattttc cgataatcag
      901 aaaaaaagta ttaacggatt tctgcaaaaa ataaagaaaa aaatcaatgg agaaccctaa
      961 tataaaaatc aggaaaaaaa tattgattac tcctcttagg aagaccatta gtcagtccct
     1021 cccggctggc tttttcgctc tgtactcagc ttcatcaagc cccataaaaa tccagaccat
     1081 atatacgatt gtgccgatta atgcttgtct gcttccccaa agacctaaag cgtagatgat
     1141 gaatggtgtg ctgaatacta gcgctctgtt gaatttgccc attagcttgc ctccttcaaa
     1201 acatctgaag gcgataaacc ttttacacga tatttgtttg cgtctttcca ctttaaaaac
     1261 caaataaaag tatggtaatg aataaatgtg gttgagtgac ctggacgaag tattccttct
     1321 gcaaattcag gaattgtttc catttccttg cagtattcaa ctaaagtagt ttttgacatg
     1381 ccgtggaact tctccaagat taaactttgt cggtaccagt cgtctggttt tactgccttc
     1441 tcagcagctt ctatcagctc tgaaagtgtt ggttttttca cttattatcc ctcctaaaaa
     1501 ttatcgttcc atactcgatg tttaacattt cttgaagctc tgattttgta agaatgattt
     1561 tatcttccaa cgactttacc tccct
//
LOCUS       DACCKU010000201         1515 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.212, whole genome shotgun sequence.
ACCESSION   DACCKU010000201 DACCKU010000000
VERSION     DACCKU010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.212"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            287..694
                     /locus_tag="IVN13_002957"
     CDS             287..694
                     /locus_tag="IVN13_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013376.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IVN13_002958"
     CDS             708..1109
                     /locus_tag="IVN13_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013377.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IVN13_002959"
     CDS             1111..1482
                     /locus_tag="IVN13_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013378.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DACCKU010000202         1497 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.213, whole genome shotgun sequence.
ACCESSION   DACCKU010000202 DACCKU010000000
VERSION     DACCKU010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1497
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.213"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(1..308)
                     /locus_tag="IVN13_002960"
                     /pseudo
     CDS             complement(1..308)
                     /locus_tag="IVN13_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(562..1116)
                     /locus_tag="IVN13_002961"
     CDS             complement(562..1116)
                     /locus_tag="IVN13_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7013379.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          485 a          257 c          155 g          600 t
ORIGIN      
        1 caaacctata taatcatgaa tgctggcaaa aattttttta agtcaagttc taaatcttct
       61 tcgaaaacac atttaatttc tcctactgat aattgatact ctatattaca tgacttaatt
      121 agttttttag atcccccctc cagataggca tatgtttcaa taattatgtt ctttttattg
      181 cttggcaaat ttccgttatt tattgcataa attaaaaatg actgtctatt tagcccataa
      241 tctaatacaa cttcttcata ctcatatttc tttaaatatt taatatgaaa atatgctgaa
      301 ttaatcaaaa ctcgagtgtt actgttgtta tttattgttg ttctattgtg tataaaattg
      361 tataggttgt catattttct tggtttatta aaatcattct ctatataata atcattatca
      421 actacaacat tttgttctat ctctggtatt ctattgtaaa tgttactaaa cgttttaaat
      481 acttccgaaa attcagttat cttcattaat ctcaccttct aagttttcat tatacacaaa
      541 aaatatatcc taatatctca attagcctcc caatttaaaa ccacgtaacg atgcccctat
      601 ttcatcttca gttataccaa tgtacctttt tgtaattgct tgcgagctat gattaaaaat
      661 aaacattaag gtagcaatgt ctctagtttt cttgtagtag tgatacccaa aagtttttct
      721 taatgtatgt gttcctatat catctcttcc taatttttct gctatatctg catatatctg
      781 atagacacga tcccttgtta tatgtcgacc ttgttgccga cttggaaata accaatcatt
      841 ttcttcttta ccttcagtat ataataaaat ctcttctctc aaattatcta taaaaatcgg
      901 ttttcgctta cctgttttat gttcaacaat aataggctta cttttacctc gtatgtctcc
      961 aacttttaat cctataatat ctgacatacg taacccatta ttgattccaa acaaaaataa
     1021 aaaactatct ctttctggat tacttgttct tcttagccaa aactgaaaat cttgtatctc
     1081 ttcatttgtc cttaacggtt gtacattata gctcactatt gtttcccttc ttaaaaatat
     1141 tctgagttca tgattatata tatattttac cataatcatg aacttatata tatactctgt
     1201 taaaagtgaa gaaaatcaag gggtttagtt tatgatttta tatcatttca gtaactcaga
     1261 atattccgac ttttttgtac aaaaaaatta accttactta tatgaagatt ctaagctctt
     1321 atttaaattt tagacgggca gttatccgta gaagttctta gaatagctaa tttacataat
     1381 taaattatat gaaatttaaa tttgaacaat atactaagaa ctccccttac tataaattga
     1441 aataaattag catatgaata accgtgctac caagcttttc attaataaaa tattttt
//
LOCUS       DACCKU010000203         1456 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.214, whole genome shotgun sequence.
ACCESSION   DACCKU010000203 DACCKU010000000
VERSION     DACCKU010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.214"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            109..1041
                     /locus_tag="IVN13_002962"
     CDS             109..1041
                     /locus_tag="IVN13_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAP7013380.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1456
                     /locus_tag="IVN13_002963"
     CDS             1113..>1456
                     /locus_tag="IVN13_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013381.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          228 g          419 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaaga
//
LOCUS       DACCKU010000204         1455 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.215, whole genome shotgun sequence.
ACCESSION   DACCKU010000204 DACCKU010000000
VERSION     DACCKU010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.215"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            75..1415
                     /locus_tag="IVN13_002964"
     CDS             75..1415
                     /locus_tag="IVN13_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7013382.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          321 c          284 g          388 t
ORIGIN      
        1 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
       61 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
      121 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
      181 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
      241 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
      301 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
      361 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
      421 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
      481 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
      541 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
      601 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
      661 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
      721 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
      781 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
      841 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
      901 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
      961 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     1021 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     1081 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     1141 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     1201 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     1261 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     1321 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     1381 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     1441 ttgaatttct tcacc
//
LOCUS       DACCKU010000205         1424 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.216, whole genome shotgun sequence.
ACCESSION   DACCKU010000205 DACCKU010000000
VERSION     DACCKU010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.216"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IVN13_002965"
     CDS             complement(150..1337)
                     /locus_tag="IVN13_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP7013383.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACCKU010000206         1415 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.217, whole genome shotgun sequence.
ACCESSION   DACCKU010000206 DACCKU010000000
VERSION     DACCKU010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.217"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..401)
                     /locus_tag="IVN13_002966"
     CDS             complement(<1..401)
                     /locus_tag="IVN13_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP7013384.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSA"
     gene            complement(423..1016)
                     /locus_tag="IVN13_002967"
     CDS             complement(423..1016)
                     /locus_tag="IVN13_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013385.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(1017..1349)
                     /locus_tag="IVN13_002968"
     CDS             complement(1017..1349)
                     /locus_tag="IVN13_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013386.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
BASE COUNT          409 a          283 c          218 g          505 t
ORIGIN      
        1 tccgcacttt tatttcggtc agtcgctttt cgttcattat ctgatcgatc atttaattca
       61 tctaatgcgc ggttgatttg atcaatcact ttttgctttt catctgtgct aacatctagt
      121 gtggccactg tatgaggatt gttcatgaga ttggccaacc aaaaaagagt aggatttttt
      181 gtgtaatgat aaactgtcac acatcgtgaa taaccatttc cataggtcac atattcttca
      241 tcaaaattca acccaccttg tggcatcact tgttttaaga aatctaaatc ataacctttt
      301 gattttaaaa tctctatttc acgattacca agttgtttta atggatcaat caccttttga
      361 ttactttgcg ttgaaaaagc tgtttttatc acatctagca tgaatcattt cctccatctt
      421 ttttattcgt cattcatgtt gttcattcga taaagaatct taatcaattc ttttttgtag
      481 agtggtttta accctaaaat gccactagat cgcattaact gacgatttct agtttgtaaa
      541 tccttgattg tctttccttc aataacgaaa aagaagctta agtcaggacg ttcttgttct
      601 gcccgtttta attcctggat ctcctctact aatttctttt catattttct taatcgctgt
      661 agatttcttc ctagtgactg ttccatacga tttttatcta ataactgccg cttttgcacg
      721 atttgttccg ttagatccgt ctctgtggtc aaactaacta acttaattgt tggcacatac
      781 gtacgcatga cggtggtcaa ctgattcatt cgttctagtt tctcttgttc atttaatccc
      841 tccacgtcag tggaaacgac acgataataa cgctgaaaat gatcttctct agtaattaag
      901 actccctctt ctgtacagga tttatatgga aataagtcca agatagagcg ctcttctggt
      961 tttccaaact gcaaaatgga tttcttatca actgaatttt gtcctttgac tcgcatttat
     1021 tcatcctcct tatacgtacc atgataattt cggttaggat ctaacgcatg atagtggttc
     1081 ttatccgtaa agaaaataga ttcgataatc gcaaaaattt ggcgttgacc agggttatta
     1141 tagggtcgcc aaacaatcac taacgctatc gcaagccata atccataatt caagagtgca
     1201 aaaaagagtg gtaagtggac aagattattg actattttcg cccaaaaaaa gcagccacca
     1261 agcgttaata gatctaaaaa gaaaaaggcg aaaaacttcc attccccact aaatcttgga
     1321 ggatttttca tatgaatgta tttatccata atataaccat accccttata ttttgtattt
     1381 ttagaaataa aaaacactgt gatttctcac agcgt
//
LOCUS       DACCKU010000207         1388 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.218, whole genome shotgun sequence.
ACCESSION   DACCKU010000207 DACCKU010000000
VERSION     DACCKU010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.218"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(131..799)
                     /locus_tag="IVN13_002969"
     CDS             complement(131..799)
                     /locus_tag="IVN13_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP7013387.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            933..1220
                     /locus_tag="IVN13_002970"
     CDS             933..1220
                     /locus_tag="IVN13_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013388.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
BASE COUNT          517 a          227 c          228 g          416 t
ORIGIN      
        1 aacaaactat tttcgcagat cgtttgcaaa aatagtttat ttttttataa agaagatttt
       61 actgaataat atcattttag tttacataat gatatttctg taaattaaat aaagtagtga
      121 aaaataagaa tcagtgctct aactgttcaa gaggatcaag tggttcaaat gtttctgtag
      181 tctgttttgc ttccaattgg ggcaaaacag atccaatttc cagatacttc actttattca
      241 atgtataaga tagaatagaa cagctaagta atgtatacag aaaatgtgtg agatccaaat
      301 aaagcaagga gacaactaaa ataccaaaag taatcaagaa aagacttttg ccaatcttaa
      361 tatgaaaata tttttcaatg gcttgataaa tgatatctgt tccgcctgtg cttccgccga
      421 atcgaaagac gagaccgcaa ccaatgccag aaagaatacc tgtcatcaaa ctattgatca
      481 gtagactatc tacagcaaaa tgtatatgaa ggctctccca taaatgtaaa aagaaaacta
      541 gcgcaaatgt tccgacgatc gtccggagaa aagcgtgttt tccaaaaaga aacaatgaga
      601 taagcaataa aggtaaatta aaaagcagtg aagtgacaga aggagcgaat ccaaaaagat
      661 tatgcaaaag caaagcaata ccagtaaccc caccatctgc cagcttagat ggaatattga
      721 ttcctgtcac agctaatccg tagattcctg cgcctaaaat gattccgcac aattctgtaa
      781 ataaatttaa ttttttcatt tctagcctct tttcttttga aaattttcag caacttcttt
      841 attatactga cttatactct tacttcaaga ggaaaatgaa aaaaaattat ttttggctgt
      901 ttctttttat ttatcaaata aggagtggaa aaatgcgagc agcagactta ttgacaattc
      961 ttacaaaatc acccaaaaaa acagagtatc aaggagtttt tttggctcaa gaacaaaaac
     1021 tggtgcctgt gacaatggtc cagataaata aagcaaatca atttatcttt ttatttgaac
     1081 acggcaaagc accacttgct atgaaagaca tctttctttt tttgatgaag aaccgtgaga
     1141 aagctgttta ttatcagtca aaagaagaac tcattcctct ttatggagtg aaagagatag
     1201 aaaataaagt ggtgatataa gtaaaaataa gaaaacaaga cgtttatgat gtcaaaacaa
     1261 ccatttttta catttttggc aaaaaagtgt catgatacag tataatattc gttgaacagg
     1321 ccaataaagg tgttctcccc cacgagattt ctactttatt ggctgtttct ttttattaac
     1381 gccgattg
//
LOCUS       DACCKU010000208         1368 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.219, whole genome shotgun sequence.
ACCESSION   DACCKU010000208 DACCKU010000000
VERSION     DACCKU010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.219"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            48..1181
                     /locus_tag="IVN13_002971"
     CDS             48..1181
                     /locus_tag="IVN13_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP7013389.1"
                     /translation="MWVFGTSAGLITILITVFVTLFLILAAGSIDNSDSSSGGEAFTG
                     EYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLLPSVILSQYGYESAFGTSASARN
                     DLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPNSKAAFSYYGFMVATQSNFNACV
                     GNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSIINTNKLMEYDEFAIKHWREGGGT
                     GGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEFRPNGFHDGLDFGSVDHPGSEIH
                     AVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQEFANSTGNSRVKVGDQVKVGQVI
                     GIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLPFLNSSKK"
BASE COUNT          413 a          218 c          340 g          397 t
ORIGIN      
        1 gtttaatcaa acagtatgtt aagcgtcgtg caaagcggcg cttatttttg tgggtgtttg
       61 gtacttctgc aggtttgatt actatattga tcactgtgtt tgtgaccctt tttctaatac
      121 ttgcggcagg ttctattgat aattctgatt caagtagtgg tggcgaagct tttacaggag
      181 aatattcgga aggcttaccc atttataaag aaatcaaagg aagaggccct ttttctgatg
      241 agattgcgca atatgctgta ggtgcagcag tgaagtataa attattacca agtgtgatcc
      301 tttcgcaata cggttatgaa tcagcgtttg gtacgtcggc gtccgcaaga aatgatctca
      361 attattttgg gattacgtgg tttgatgggt gtctgtttcc taaaggaact gcgcgtggaa
      421 ttggtggcat tgaaggtggc tggtacatga agtttcccaa ctcaaaagct gccttctcct
      481 attacggctt tatggtagcc acacaatcaa attttaatgc ttgtgtcgga aataaaagcc
      541 ctggcgcaag cttattgatc ttaggacgag gagggtatgc cgccgcagga attactgaag
      601 acagtgccta ttataaaaat tgtatgtcta ttattaatac aaataaattg atggagtacg
      661 atgagtttgc gattaaacac tggagagaag gcggcggaac tggtggtacg attacaggaa
      721 gctggggcaa tccattccca ggaagtagct tagataaaaa ttcgttcagt ggaggacaac
      781 tttttgggaa aaatccagga ggagaattcc gacctaacgg gttccatgat ggactagact
      841 ttggttcagt cgatcatcca ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt
      901 acgttggcaa tcctggtatt tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg
      961 gcttgaatat ggtgtatcaa gaatttgcaa atagtacagg aaattctcga gtgaaagtag
     1021 gcgatcaagt gaaagttggt caagtaattg ggatacgaga tacagcacac ttgcatttag
     1081 gatttactcg aatggattgg cgtcaagcac aaggacatgc attcattgat gatggtacat
     1141 ggatcgatcc gttaccattt ttgaatagca gcaaaaaata gatcaggaga aaaaatatgg
     1201 aaaataaaac cttaaaaatc gtcttaacag ctgcaattag cgttagttta atcggcaata
     1261 ttgcgttgtt ttggcagctt aaagaagaac aagagacaaa tacctataaa attgaagaag
     1321 tggctaaaaa cgcgtctgat aagctcaaaa aaaattcttc tgaagcac
//
LOCUS       DACCKU010000209         1348 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.221, whole genome shotgun sequence.
ACCESSION   DACCKU010000209 DACCKU010000000
VERSION     DACCKU010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.221"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            123..1292
                     /locus_tag="IVN13_002972"
     CDS             123..1292
                     /locus_tag="IVN13_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7013390.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          484 a          218 c          240 g          406 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacacataat tcttgacgct acctttaa
//
LOCUS       DACCKU010000210         1320 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.222, whole genome shotgun sequence.
ACCESSION   DACCKU010000210 DACCKU010000000
VERSION     DACCKU010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.222"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..731
                     /locus_tag="IVN13_002973"
     CDS             <1..731
                     /locus_tag="IVN13_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013391.1"
                     /translation="EYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFY
                     IDSDVIFNKRINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTV
                     ESLETINTNSGGARWIINLPKANYWKKVYLDSNRLYRYLRGQKTNVQIWTAEMWAQLW
                     NMMYFNIGPKVHEELNFCFATDPIEKFKEVKILHNAGVTTNDEDLFFKGRYVTSTPFD
                     EDLSFVNKKKCSYAYAKAIKAVVR"
     gene            728..1039
                     /locus_tag="IVN13_002974"
     CDS             728..1039
                     /locus_tag="IVN13_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013392.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPDSLEYGLLIERCRYDWNRALGEFEQNFASELLGFIQHYALQEYIAGDGNG
                     E"
     gene            1029..>1320
                     /locus_tag="IVN13_002975"
     CDS             1029..>1320
                     /locus_tag="IVN13_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HAP7013393.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIY"
BASE COUNT          472 a          185 c          271 g          392 t
ORIGIN      
        1 aggaatatgg cgttgaagtt catgtgtacg atgatctgcg ggacgacaaa gagtatattc
       61 cttcgattaa accatattta tggtggaaat atttagaaga agatcattcg cgtgaggacg
      121 accgatattt ctatatcgat tcggatgtca ttttcaataa aagaattaat ttgcgcaaat
      181 tgccttctaa agatgatgtt tggtattgta gcgactgctg tagttatcta agtcttgatt
      241 atattagaag ctgtgaaaac ggagaaaata ttctaaaaga tatggcaaat attgtaaatg
      301 ttacagtaga atctttggaa actataaaca ctaattcagg aggcgcacgg tggattatta
      361 accttcctaa agcgaattat tggaaaaagg tttatctaga ttctaatcgg ctatatcgct
      421 accttagagg gcaaaaaaca aatgtacaga tttggacagc cgagatgtgg gcacagcttt
      481 ggaacatgat gtatttcaat attggtccta aagttcacga ggaattaaac ttttgttttg
      541 ctactgatcc aatagaaaaa tttaaagaag taaaaatctt gcataacgct ggagtaacaa
      601 caaatgatga agatttattt ttcaaaggga gatacgtgac ttccacgcct tttgatgaag
      661 atttatcatt tgtaaacaag aaaaaatgct cttacgcata tgctaaagca attaaggcgg
      721 tggttagatg acgcctgaac aagtgactga agaattgcta atagctgtga aggataatat
      781 ttacgttacc tggaacgaag aagatgaatc aattaaaaag atgatagcta aaaatgctgt
      841 ttatttacaa agtaaagtga gtacaactct ttctttttct cctgatagct tagaatacgg
      901 attgctaatc gaaagatgta gatacgactg gaatcgtgct ttaggtgagt ttgaacaaaa
      961 tttcgctagc gagttattag gtttcattca acattatgcg ctacaagaat atattgcagg
     1021 tgatgggaat ggcgaataat cgtagactcg aagaaacgtt caacgatggt tggttaaaga
     1081 ttttaacgca aaccaccaaa agaaatgaac taggaaaaaa gattggtgta gaagatacag
     1141 aaatcacttc tttaaaattt agaaatcttt ccatgagaga tagtgatata acagctatgg
     1201 atgcgatggg atcgaaatta actaagaaag taaagactcc atttcatcca atcgccaaga
     1261 aatttaataa agatcaatat tttatcgtaa tcgatagtat gcgttacaac gttatctatg
//
LOCUS       DACCKU010000211         1288 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.223, whole genome shotgun sequence.
ACCESSION   DACCKU010000211 DACCKU010000000
VERSION     DACCKU010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.223"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>1288
                     /locus_tag="IVN13_002976"
     CDS             <1..>1288
                     /locus_tag="IVN13_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7013394.1"
                     /translation="LADGQKGDNRLKTLKEIQQIYPQIPESKGSFPGYGGIPITHYKK
                     YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAERQSSMVVNDPKTELYVASKDE
                     LEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANAS
                     DNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYYPDPENEF
                     KQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFTLTKIAKMTS
                     QSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGKKFHEERVKVGYRGFCEINFDC
                     HLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKIVYRKKRGETHLPELEKEAVLT
                     LDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI"
BASE COUNT          469 a          215 c          250 g          354 t
ORIGIN      
        1 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
       61 tatccgcaaa taccagaatc aaagggaagt tttcctggtt atggcggaat accaattact
      121 cattataaaa aatactggta tattcagacg gatacagtaa atacttgtat tgttggtact
      181 tcccgctcag ggaaaggcca gatagaagtt ttagcaacca ttgacaattt gtcgcgtgca
      241 gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag aattatatgt tgctagtaaa
      301 gatgaactag aagctagagg gtatgatgtg tatgccttta atatcaccga tccactgcaa
      361 tcaatgagtg ataatccatt ggcactcatt gtgaaatact ggaagcgagg agacatagac
      421 accgcaacac agttaaccaa tacttttacg aatacgattt attatgatgc aaatgcttca
      481 gacaataaat actttaacga aaatgcgcag aaagctgtca atgcgcttat ttttgcgtta
      541 ttggaacaag cggatcaaac aggggattac tccaaattaa caccaaataa tctcgttgaa
      601 ctactttcag aaattggcgg ttttaattat tatccagatc ctgagaatga attcaagcag
      661 ctcaatgcct tagatgagtt catgaatcag ttaccccctg gaaatatagc aaaaaaacaa
      721 tatggggcaa caaaaattgg atctgataaa gcgaaaggga atatcttgtc tacggcaatt
      781 acaggattga atccatttac cctaacgaaa attgcgaaga tgacttctca atcgaccatt
      841 gattacaagt ccattggttt tccgaagtat ttggatctca aattacatga aagcctatta
      901 aatcaacgca ttgtcgtgga attttatcat aagggaaaga aatttcacga ggaacgagtc
      961 aaagttggct atcgtggttt ttgtgaaatc aactttgatt gtcatttaga gcagggagat
     1021 catgtttgct taaaatacaa tcatcaagga aaacaatatg ttgcttggta tgagttttct
     1081 caaaaagtat tgaaagacga agagggaaaa atcgtttatc gaaaaaaacg aggagaaact
     1141 catttgccag aactagaaaa agaagccgtt ctaacattag atgaggaaaa aagtaactta
     1201 ttagtagagt ctattcggat gcactatacc gataaaccaa tggctatttt tatgttaacg
     1261 cctgattatg atccatctaa ccatatca
//
LOCUS       DACCKU010000212         1276 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.224, whole genome shotgun sequence.
ACCESSION   DACCKU010000212 DACCKU010000000
VERSION     DACCKU010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.224"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            4..>1276
                     /locus_tag="IVN13_002977"
     CDS             4..>1276
                     /locus_tag="IVN13_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013395.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIY"
BASE COUNT          529 a          206 c          281 g          260 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctata
//
LOCUS       DACCKU010000213         1254 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.225, whole genome shotgun sequence.
ACCESSION   DACCKU010000213 DACCKU010000000
VERSION     DACCKU010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.225"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            153..695
                     /locus_tag="IVN13_002978"
     CDS             153..695
                     /locus_tag="IVN13_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013396.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            851..>1254
                     /locus_tag="IVN13_002979"
     CDS             851..>1254
                     /locus_tag="IVN13_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP7013397.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDGMATLFLTKIIRCCECCG"
BASE COUNT          506 a          142 c          242 g          364 t
ORIGIN      
        1 gttcttcgtg ttctcttaga cataaaaatt cctcctggta tgttttcttc tagtctacac
       61 accttaattt ttctgtctag ttaattgtag cctatccagt ttggaaaaat aaaaaattat
      121 atttcatcaa cgtttcataa aaggtgtgta gaatgttatt gaaagtagct ataaaatgct
      181 attgtgtaaa aaataaagga ggaataattt atgaaaaaat agtaccaaga ggagcatgcc
      241 tattacttgc attatgtttg agcttaagcg cagttgggtg tcaaaaaagt aatgaaaaca
      301 ataaaataaa agaaggacaa tccataagtt ctgaagagga aggcatgtca attaatgcaa
      361 atggcaaaaa tgaaactttt aagccatcag attatacatt ggaggcaaag aaagaatatg
      421 tttatgaata tttagggctt aaatttaaac tctcagacaa atttagaaat tatatagctg
      481 ataaaaagat agcgatgtta gatgatcaaa gtcctataga taaagaacta aagtatgcta
      541 ttttgacatt tgagaaaatg acagaagaac aaaagaatgc agttatagaa aagatgggcg
      601 atggatataa aaattggcaa aatgagcttg aaagaattgg gactattggt atatttgaaa
      661 aaaatacatc agaagaaaaa aatctaaaat tataaaatgt gatactcata ctaaaatagg
      721 agtttcaagt gatggaaaat acgattgcta tttaagcaca aatagcggag ctgaaagtaa
      781 tcttcttgat gaattaaaaa gaactgagat tcaaattata gataaaaaag aacgtcctaa
      841 aaatggtttt gtgctatcag aaaaaactga tttagaaaat acagaagcat ttaacaaaga
      901 atcggttaaa gatttacgta aactaccaac aaaggacata aatggcaagg attttacaag
      961 taaagatttt gagaagtatg atcttactat ggtaaatgta tttgcaactt ggtgtacagc
     1021 ttgtgtaaag gaaattcctg atttggttga ggttcagaat gaaatgaaaa gcaagggtgt
     1081 aaatatcgtt ggagttgtta cagatcctgt tgatgataac ggtgaaaaca aggaagcaat
     1141 tgaaaagtca aagttaatac atgaaaagac aaaggcttca tatcctttct taatgccaga
     1201 tggaatggca acactttttt tgacaaaaat tataaggtgt tgtgaatgct gcgg
//
LOCUS       DACCKU010000214         1232 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.226, whole genome shotgun sequence.
ACCESSION   DACCKU010000214 DACCKU010000000
VERSION     DACCKU010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.226"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            82..>1232
                     /locus_tag="IVN13_002980"
     CDS             82..>1232
                     /locus_tag="IVN13_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAP7013398.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNA"
BASE COUNT          407 a          235 c          239 g          351 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcacgac aaaataaaca
       61 actgagagga cgatatgcta tatgaatgat tctatcaaaa aaatgctgcg attaatagag
      121 aaagatttga tgattacaga ggtctcttac gagacctttc agaagaaaaa gacgttgatc
      181 gtcgatgctg ttttctcacc tgctccccat acctgtagaa attgtggttc tactgtggta
      241 gatggaaacg ggaaagtaat tgtagtgaaa aatgggaaga aggaaacgat tgtccgcttt
      301 gaacaataca atcacatgcc tttggttatg cgcttaaaaa agcagcgcta tacctgtaaa
      361 aactgtcgaa cccattggac ggctcaaagt tattttgttc aacccagaca ttcaatcgcg
      421 aatcatgtca gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaaatt gtcaggtatc tttgaccacc gttattcgta cgttgaaaga gtttaaaagc
      541 tacttaccaa agcaatctaa gaggattctc cccagagtat tgatggttga tgaatttcgt
      601 tcgcacgctt ccatagaaga taaaatgagc tttatttgcg cagatggcga aacaggacaa
      661 ttaattgatg tcttgcctac gcgtaaattg cctcgattaa caagctattt cttaggctgc
      721 gctaatcctg aagaagtaaa atttttagtc acagacatga acgccgccta cttccagctt
      781 accaaacgta ttctgccaaa tgcgaaagta gtgattgatc ggtttcatat tatcaaacac
      841 atgaatcaag cctttaatga gttgcgtatc cgtgaaatga atgaacttcg taaagtagga
      901 cagaaaagtc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagttttc gagcaccaaa atacccattt
     1021 cttacggaag cgatgatgat tgatcgattg cttggctttt ctacgtccct gaaggaggcg
     1081 tatccctatt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacggct ttcgggaaaa gctccaaaac
     1201 cttctgacct atgaagaagg catcaccaac gc
//
LOCUS       DACCKU010000215         1215 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.227, whole genome shotgun sequence.
ACCESSION   DACCKU010000215 DACCKU010000000
VERSION     DACCKU010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.227"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            414..947
                     /locus_tag="IVN13_002981"
     CDS             414..947
                     /locus_tag="IVN13_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP7013399.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          389 a          226 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttacc
//
LOCUS       DACCKU010000216         1201 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.228, whole genome shotgun sequence.
ACCESSION   DACCKU010000216 DACCKU010000000
VERSION     DACCKU010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.228"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..424)
                     /locus_tag="IVN13_002982"
     CDS             complement(<1..424)
                     /locus_tag="IVN13_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP7013400.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQI"
     gene            complement(533..>1201)
                     /locus_tag="IVN13_002983"
     CDS             complement(533..>1201)
                     /locus_tag="IVN13_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7013401.1"
                     /translation="LYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEHLFIPI
                     EGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNLNRKEN
                     RLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKITVYKN
                     VSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHELAAFVR
                     VSSI"
BASE COUNT          374 a          213 c          210 g          404 t
ORIGIN      
        1 gaatctgttt tgattttgtt ccatttgaaa tttctctcac tcgctttttt gtctcatcat
       61 caaataaaat gaaagggtct tctaaacgat cgataaatac ttccattgtt gttgttggta
      121 gatgataaga agtcatgaca ggcactgcac caaggtaaga cacagaaaca gctagaaggt
      181 atgtgtcaaa agcggaactc ttataaataa taactttgtc tccggatttc actccaagat
      241 gagccaattg ataagcccgt ttcaacacac tttcgcagca ttttttgtaa gttgtttcaa
      301 gtcctaattc tgggaaagct gaaagtggct catcaaagat aatcggggtc tcggaaaagt
      361 tagctgcagc ttcttgaaaa ttcgtaaata agttcaaagg ggaatacgtt atggtctcca
      421 tcattcacac tccattatct tttgttaagt aatatacaaa ttcaagataa tcgtaacata
      481 atcctttata taataacata gtttattgtg aaaaaaagat catacatttg ttttatatgg
      541 aagaaacccg aacgaatgcc gctaattcgt gataagaaaa atcaaccatc ttttcccaga
      601 tatcttgaaa agaatgggat gagaggtatc gattaccaca taaataattt accgcgtcag
      661 catcatctaa atatagttct gcataataac gatatgtttt tctgcatagt tctggcgata
      721 cgtttttata tactgtaatt ttgctcaaaa tttggcgaat agtccgagga gaaatttgct
      781 gatcatgtga ggaacgaaaa acaaaagaat tttcgtccgt attttgtgct gttaacttaa
      841 aatagttctc cagaaatggc atcgcttctt ttggtattgg tactttattc agtctgtttt
      901 cttttctatt cagattaaga atctgcttgg tcaaagatac atctttacgt ttaagttcta
      961 ctacttcatg gatctttaat ccgcaataca gaagaatggc agtaattgca aggttcctcg
     1021 gtctattctt ttcttcaaag ctagcttctc tgcttgtttg aaagccttca atcgggataa
     1081 acaaatgctc aaaatctttg aaaagttctg catcgatata tcgcggagct gctttggttg
     1141 tttccggtaa acttgtaccc aaaagagaaa tctcttcttc aatcagtcct ttttgataca
     1201 a
//
LOCUS       DACCKU010000217         1192 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.229, whole genome shotgun sequence.
ACCESSION   DACCKU010000217 DACCKU010000000
VERSION     DACCKU010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.229"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IVN13_002984"
     CDS             <1..1076
                     /locus_tag="IVN13_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7013402.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACCKU010000218         1168 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.230, whole genome shotgun sequence.
ACCESSION   DACCKU010000218 DACCKU010000000
VERSION     DACCKU010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.230"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..1163
                     /locus_tag="IVN13_002985"
     CDS             <1..1163
                     /locus_tag="IVN13_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013403.1"
                     /translation="QGKGTLLEQLESVQSTNWHELAQEDLLATFEERQQETDQEINEY
                     ATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVAKINNFTQTNQE
                     ALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTTTEVLMEKYEKI
                     QQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQDLAEQQMAIEKQ
                     RLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTPSPQ
                     RSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEEAQEQARKEYEQ
                     QLEELLNRTNQSTTESSMENSKESDPSKAKEGSSDSTVQSNTSESSESNEVK"
BASE COUNT          507 a          197 c          232 g          232 t
ORIGIN      
        1 cacagggtaa aggcacactt ttagaacagt tagagtctgt ccaatcaaca aattggcatg
       61 agcttgcaca agaagattta ttagccacct ttgaagaacg tcaacaagaa acagatcaag
      121 aaattaatga gtatgcgacg gaacagtcgt ctaattggga gaaagcaaaa gaggaactaa
      181 ataaagaaga acagcgagtg attgaagaag aagtacaaaa aattaaagag cgatacgaaa
      241 agaaacgcga caatgcgtat tccttgtgtg tagcaaagat caataatttt actcagacga
      301 atcaagaagc gttatcgaaa gaaaaagaaa acttgttaaa agaacgtatt aaagaacgaa
      361 aagaagaatt ttaccgaggt ttgcgtacag ataagctaaa gattagcaaa gaattaaatg
      421 aacaactgaa taagctatat caaacaacga ctgaagttct tatggaaaaa tacgaaaaga
      481 tccagcaaga attacaagtg caaattccaa aatggaaaaa agagtatgaa attcaacaac
      541 aagaattgaa agaacaagaa gaaaaacaac ggcaaaaaga aaaagagcgc gaacaattag
      601 aactaaaaaa acgtgaacag gatcttgcgg aacaacaaat ggcaatagaa aaacaacggt
      661 taaaccaaga aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa caaatggata
      721 cattgcgaca agctcagtta aattatctag cacaacctca accaacgcct tcagtagctg
      781 ctgttcaaac cccaatgaac aaccaaacaa atctaactcc ttcaccacag cgttccttta
      841 acggatggat ggtgggatgt attgcaagtt taagcttgct tctaggtgga ggaacagtat
      901 ttgcttttaa tcatatcaat caatcttcac aggctgattc tgtggccaca acgacgtcaa
      961 tcaaggaaga agcccaagaa caagcaagaa aagagtatga acaacaatta gaagaattat
     1021 tgaatcgtac caatcagtca acaacagaat caagtatgga aaatagcaaa gagagtgatc
     1081 ctagtaaggc taaagaagga tcaagcgatt caactgtaca atcaaacact tccgaaagtt
     1141 cagaatcaaa tgaggtgaaa taatgcgt
//
LOCUS       DACCKU010000219         1128 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.231, whole genome shotgun sequence.
ACCESSION   DACCKU010000219 DACCKU010000000
VERSION     DACCKU010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.231"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            93..323
                     /locus_tag="IVN13_002986"
     CDS             93..323
                     /locus_tag="IVN13_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013404.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            869..>1128
                     /locus_tag="IVN13_002987"
     CDS             869..>1128
                     /locus_tag="IVN13_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013405.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIG"
BASE COUNT          458 a          148 c          196 g          326 t
ORIGIN      
        1 tgaaagatca agaagagatc attcaaaaac ttcaagaaaa aattgatcgt atgacaaaag
       61 aaaagattta gaaggacagg tggagtgaaa aaatgaagcg aacaaacttg gctgacaaag
      121 atactttcgc tgagcagaaa gtattgatta aagcctatct caaagttgta aaagctaaag
      181 aaatatgtcg agaagtgaat ttatctaaca caacaatatc tgagttgaag aatggtcgaa
      241 agaatttaga tactttaaaa ttcagatcat ttgaagctct atatgtagct gctaaaaaat
      301 attttacagc tgataacaaa taaaaaacag gagtatttta tgtgaaaaaa gtatataaaa
      361 aatgcttatg aatcagctat atcaccttaa ccatttgtta atgagatggg agaaaacatt
      421 aaccacgatt ctaacgcaaa catcaagcac ataaaacatt taacactcct cctttctggc
      481 tgaagatttc aaccaaacag gaagacagcg tatcaattat agcatacact ttttcttaaa
      541 ttcatttttc atatgacaaa atatttcctt ctaatcgaca aagttagaag gattttttat
      601 tgtctaaata cacaaataac cttaattttt aaagagcata aggaaacgtg atatcacttt
      661 ctcacgaaag gtgttaggta ggctgtgcct accacgtttc taagaaagat aaatgatatc
      721 aaaattttgg atttgtgtgt agatttgtgg gtaggatgaa agcgaatgtg ggcagtaaaa
      781 taagcgtttg tgtgtagcaa aatcaaaaaa tgtgtaggga tatgtgtagc tacacatatc
      841 cctacacata ataaatttgg aggaaaatat ggacttatta attagaaata ttgaagaaaa
      901 gtatattaat aaaattgaca aacgttgtga agaacttacg gtcaagactg gcaaaaaatg
      961 gagtcgaaat caatacctaa aagttttgat cgaaaatgat tttgatcacg ctcttctgaa
     1021 ttataaaaaa gaccaatttg atcggttgtt agaaaaattt gtggatattc aaacttataa
     1081 tacgaaaact ttagaagaat atattgcaac aaataatcaa ttaattgg
//
LOCUS       DACCKU010000220         1057 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.232, whole genome shotgun sequence.
ACCESSION   DACCKU010000220 DACCKU010000000
VERSION     DACCKU010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.232"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            129..983
                     /locus_tag="IVN13_002988"
     CDS             129..983
                     /locus_tag="IVN13_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP7013406.1"
                     /translation="MEKEKLKILEELRRILNNKNEAIIILNNYFKGGVGKSKLSTMFA
                     YLTDKLNLKVLMIDKDLQATLTKDLAKTFKVELPRVNFYEGLKNGNLASSIIHLTDNL
                     DLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIIIDTVPTPSVYTNNAI
                     VASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFVPYLVDTDSATIKSN
                     LEELYKQHKEDNLVFQNIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERI
                     IQLENEKE"
BASE COUNT          409 a          153 c          174 g          321 t
ORIGIN      
        1 aagagggctt ttatatatta atcacaaatc acttatcaca aatcacaagt gattaatcac
       61 aaatcacttg tgatttgtga ttcttaatga tacaatatta ctatacaaaa aaagaatggg
      121 gcgtagttat ggagaaggaa aaactaaaaa tacttgaaga attaagacgt attttaaaca
      181 ataaaaatga agcaattatt atcttgaata attactttaa aggtggtgtt ggaaagtcca
      241 aattatcgac tatgtttgct tacttgacag acaaattgaa tttaaaagtt ttaatgatcg
      301 ataaggactt acaggcaaca ttgacaaaag acttagcaaa aacatttaag gtagaattgc
      361 cacgtgtcaa tttttatgaa ggactgaaaa atggaaactt ggcttcttct attattcatt
      421 tgactgataa tttagacttg attcctggca cgtttgattt gatgttactg ccaaaattaa
      481 ctcgctcatg gacgtttgaa aacgaaagta gattgcttgc tactctttta gcacctttaa
      541 aaagtgacta tgatctcatt attattgata ctgtaccaac gccaagcgtt tatacaaata
      601 atgcaatcgt ggcaagtgat tacgttatga tccctttaca agcagaagaa gaaagtacaa
      661 acaacattca aaactatatt tcctatttga ttgatttaca agaacaattt aaccctggac
      721 tagatatgat cggttttgtt ccttatttag ttgatacgga cagcgcaacg ataaaatcaa
      781 acctggaaga actgtacaag caacataaag aagataactt ggttttccaa aatattatca
      841 agcgaagtaa taaagtaagt acctggtcta aaaacggaat tacagaacac aaaggctatg
      901 acaaaaaagt tttatccatg tatgagaacg tattttttga aatgcttgag cgaattattc
      961 aattagaaaa cgaaaaagaa tagaatcaca aatcacaagt gattaatcac aaatcacaaa
     1021 tcacttgtga tttgtgattg ttgatgataa aataaga
//
LOCUS       DACCKU010000221         1050 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.233, whole genome shotgun sequence.
ACCESSION   DACCKU010000221 DACCKU010000000
VERSION     DACCKU010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.233"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..414
                     /locus_tag="IVN13_002989"
     CDS             <1..414
                     /locus_tag="IVN13_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013407.1"
                     /translation="PNDNGIEVGTEKSTKIGLPKTQNLPMKDSRLNQFKENDQSFVHQ
                     SPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIVTKEAAPKPINKVHNGYR
                     PANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            475..1017
                     /locus_tag="IVN13_002990"
     CDS             475..1017
                     /locus_tag="IVN13_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013408.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
BASE COUNT          430 a          151 c          191 g          278 t
ORIGIN      
        1 ccaaatgata atggcatcga agttggaact gaaaaatcaa cgaaaattgg tttgcctaag
       61 acacaaaatt taccaatgaa agatagtcgt ttgaatcaat tcaaagagaa tgatcaaagc
      121 tttgttcatc aatcacctgc aggtactgtt caaatgaaat caagtcgttt gcctgaaaat
      181 ggaaatctga caactgtttc ttacaattta gctgaaagtg gtgaaacaga gaatgcgtcc
      241 gttcaaaata ttgtgacaaa agaagctgca cctaagccaa tcaataaagt tcataatgga
      301 tacagaccag caaacgtagc atttactgca ccgacaaaaa taaatgtgac aagagaaact
      361 aaaaacagga acaatgaatt gaaagaacaa ctattgaagg atatcaaacc ataaaaggcc
      421 tatttaaggg cctttttctt tataaaacaa gtttaaaatg gaaaggagaa aattgtggag
      481 gatattttaa tagctacgtt tataactgca ctgggagcat ttttaacgat atttgtggtg
      541 actattaaag gatttaaaga tacggatagt ttacaaaaag atcatcagtc tattttagat
      601 ggtcaaaaaa cacataccga aagcattatt gtacaccttt cagaaggatt cacttatttg
      661 aaagatgcta tgcccacatt cacagtaaaa ttaacggaaa tatttcattc tgttgagcaa
      721 atatcgtcag aattgaaaga gcaaagacaa caaaaagatc gtcaacttgg ctcacttaat
      781 tttaggcaag aaacgataca agaatcattg gagaatgtaa atcgattaaa cgatgaaatg
      841 caacgagttc atttggaaaa tgaagaatta cgcgcacaaa attatgattt aattcaaaag
      901 aaaagaaatc tggaacaaaa aattgttgaa ttaaaagaag aaaatcgtca attgaattat
      961 caattacgac gaggaaaaaa tcgttctcgt gatttaggaa atgaattaga gttgtaatat
     1021 ttagaaaaac gctgtgagaa atcacagtgt
//
LOCUS       DACCKU010000222         1018 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.234, whole genome shotgun sequence.
ACCESSION   DACCKU010000222 DACCKU010000000
VERSION     DACCKU010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.234"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            579..854
                     /locus_tag="IVN13_002991"
     CDS             579..854
                     /locus_tag="IVN13_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013409.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
BASE COUNT          344 a          177 c          152 g          345 t
ORIGIN      
        1 ttgccgtacg tagcaccgta tagcttctta acgatctttt ttcggtagtc gtaaccgtca
       61 taggcattta atgtcgctgg caaggaatcg aaccttgcat ggttgccgaa gcattgacct
      121 agcacacatg cttagcgtct accctttccg ccacagtgac actataaaat tattcttggc
      181 tgctactatt ttttattttg cccattttta aatccaatca tatagacatt aagacagagc
      241 gcaaaaattg aaattattaa tggaatcatt tctcttcacc cacctttagt tatcgtgtga
      301 ataattaaaa aacaatagac agcaacaaaa taacattgct ttgataattt ggtataaacc
      361 actataaatt tcttttcttg caattatttt caatatatgc tagattatca accgatatag
      421 tcactgcctg tactagcgga aactagtgca ggttttttgt tctatttact caaaagttat
      481 tacgataaat taatattgtg aaaataaata ctaggcgtat aattttattt atcagcgagt
      541 ggtccgctga aatataaaac aaggatgtgc aagacaacat gtataaaccc tacatgatta
      601 gttatgatct taataaccca gggcaaaagt atgataaagt atttgaaata ataaaagaat
      661 ttggggcata tataaagcta caaaaatctt tttggttagt taaaactaat ttgaatccaa
      721 atcaaatgtg tgaaaaatta aatacagtac ttgataataa cgattcctta ttcatctgtg
      781 aactgcaaaa aaattatcaa ggtagagcta cagaggaaaa ttggaaattc attaacgaac
      841 atattttctc ttagtaagga ttagatattt tttctctgtt taaacaattg caaataccgt
      901 caatatctga attagaaaca cttacctttt gctccttgct actaccaata gcttggagca
      961 attcttgtat ttcttctgga gtgccttcta ctgataattt catttttctt ccctccaa
//
LOCUS       DACCKU010000223         1016 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.235, whole genome shotgun sequence.
ACCESSION   DACCKU010000223 DACCKU010000000
VERSION     DACCKU010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.235"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            114..326
                     /locus_tag="IVN13_002992"
     CDS             114..326
                     /locus_tag="IVN13_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013410.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            488..1006
                     /locus_tag="IVN13_002993"
     CDS             488..1006
                     /locus_tag="IVN13_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP7013411.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
BASE COUNT          379 a          129 c          165 g          343 t
ORIGIN      
        1 aaaagtgagt atctccattc ataaaataat caacatattt aaaaaatcta attagctttt
       61 ttagtcaatc taaagttctt tattctatga tataatgtta ctgtagaaag tttgtggcgg
      121 tttggcaaat ccaattgaag gagcgtggtg cctatgggct tattagctta tcggacgtac
      181 tctgtagaaa ggaaatgcct gatttgttct cattggatca aatcattgct attatcggag
      241 cagcagcagt agttattact gccgtagcga aagctagcga ttcgatttta gcttggttaa
      301 agttcaaaaa agaacaaaaa aaatagagcc gtctcaactt ttggcgaggt agacgagctc
      361 taaagcttta tttacatgcc atccgctttg aacggactac actgggcatg tgacagcatg
      421 ccctttcatt tatattatat ctcattcttt tatataatga aagtaaataa atcatggagg
      481 ataaaatatg gataaattta atagattaac attaataaat cagtttgaaa ttttaaaagc
      541 tttaaatcct aacgaagaaa aatactatgc tgaaaaaata gaaatactaa cagaaggtta
      601 cgaatatcac tatagtgaaa tatttgaaaa tatttctgat ccattgccag aagaagattc
      661 gcggtttgtt ctagacatat taaatatgta tagagatatt acttttagta aaaataaatt
      721 aaaagatata aattcaattg ataattatta tatacaattt aaaggttttg actttaatag
      781 ctctaccgaa tttaagttag cactttatgc acaatttttt attgaaaaat taaataggtt
      841 tcaagaaata gttgaagata gtgattttaa cacttttaat agccataaac cgatgagagg
      901 tacttatata aaatttctcg aaaattataa tgatttaaaa tctaccaata attatcaatt
      961 tggaaacttg tcttatgaga tgataagtaa agttctaagt ttgtgatgat aaatga
//
LOCUS       DACCKU010000224          955 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.236, whole genome shotgun sequence.
ACCESSION   DACCKU010000224 DACCKU010000000
VERSION     DACCKU010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.236"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..97
                     /locus_tag="IVN13_002994"
     CDS             <1..97
                     /locus_tag="IVN13_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP7013412.1"
                     /translation="SYRLYQCPTCGKKYTLSYGDYILIDEKGKKR"
     gene            97..348
                     /locus_tag="IVN13_002995"
     CDS             97..348
                     /locus_tag="IVN13_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013413.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            364..801
                     /locus_tag="IVN13_002996"
     CDS             364..801
                     /locus_tag="IVN13_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013414.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFIETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
BASE COUNT          384 a          149 c          185 g          237 t
ORIGIN      
        1 aagctatcgt ttgtatcaat gtccaacatg cgggaaaaaa tataccttgt cttatggcga
       61 ttacattttg attgatgaga aaggaaagaa acgctgatga aaacacaaca caaattattg
      121 actgtaaaag aagtcgttca aattttggtg gacaaagaag tcatttctga aaaagtagca
      181 gaagctttac ttattccgcg gtactggacg atagaaaccc ctttagtaga ggatgcaccg
      241 actatgctat ttgaagacct agtgaagatt gcaggatatg aactgcatta tcaagaaaac
      301 atggaaactt atgaaaacga atattggtta accaaattag agaactaaaa aggagaaaaa
      361 aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa aatggcacaa
      421 aaaatggagg atatgacgtt cctatacaac gaaacacgtg ttccaaagaa acattataaa
      481 gaaaaattgt cggtagctgt ggaagaaatg attgaatcag gtgtcgaaat gaatctcatt
      541 gcgacgtact atcgaacact ggaagaactg aaaaagcaaa atgcaaaatg gtttttccaa
      601 gccttactat gcttggaagt aggcgtcaaa ccaagcacaa tcaaaccttc tgaataccaa
      661 gctttagaac taacgtatgc gaaatttatc gaaacgaaaa aagccaagac tgtttcaagt
      721 gaatggttag attattttga aaatatcaat aagtatggcg catattacac catgaaaaag
      781 gaagacaacg aaaatgaata aggtgattta tcaactaaca acggattcca tatcaacgat
      841 ttgaaagaaa aaaaggtcaa taatcattta taccgaaaag tgagaacgtg tgaaataagg
      901 acaaaaaaag cctagtaact aagggaatgg ttcttatttc gcaccaatcg ctcca
//
LOCUS       DACCKU010000225          951 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.237, whole genome shotgun sequence.
ACCESSION   DACCKU010000225 DACCKU010000000
VERSION     DACCKU010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.237"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..782
                     /locus_tag="IVN13_002997"
     CDS             <1..782
                     /locus_tag="IVN13_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7013415.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(818..>951)
                     /locus_tag="IVN13_002998"
     CDS             complement(818..>951)
                     /locus_tag="IVN13_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7013416.1"
                     /translation="EKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT          386 a          135 c          120 g          310 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaattgaa gaaaacaaat
      481 taattaagaa taataaagat aaatacattt tcatctctaa aaatggtaac ccgatatcta
      541 ttcaagcata taatcaatct ttaagagcag tatccaaaga attaaacata gataaaaaag
      601 tttcttctca catgttaagg cattcccaca tttctctact tacagaatta ggaatcccat
      661 taaaagccat tatggatcgt gtaggacatg aagattcaaa aacaacatta aagatttata
      721 ctcatacaac taaaaatatg caaaatcaac tagtagaaaa actaggaaaa atagaaattt
      781 aaattgcccc tttttgaact gaaccccaaa agttgaacta tttaatggac tgtttccgat
      841 attctactgg agatagtcca tttaacttta gttttatcct tttgttatta taccacctaa
      901 tatactcatt taatttactt tgaaatatct caattgatcg gaatttttct c
//
LOCUS       DACCKU010000226          900 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.238, whole genome shotgun sequence.
ACCESSION   DACCKU010000226 DACCKU010000000
VERSION     DACCKU010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.238"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(238..651)
                     /locus_tag="IVN13_002999"
     CDS             complement(238..651)
                     /locus_tag="IVN13_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP7013417.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKAQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          310 a          117 c          107 g          366 t
ORIGIN      
        1 ctataattga tttatacgac tcatttttga gtcgtcagac tgcaaacaac actctaaatt
       61 taggagtttg tctacagtct gaataaattt taaatttatt gtccataatg ttattatgaa
      121 tttatagcat taagaaaaga gataagactt tacatttttt taggaatgta aagtcttatc
      181 tcttttctta attgtacaat tagtattatc tgcactactt tctaattaaa taaattttta
      241 gttattaact tgattaaaat cagagataat atcatttaat tcagtttgaa gggttgtaac
      301 gattaaatgg aaattttctt catctgtttt attatatggg gatgttttcg ttatatataa
      361 caacttttct ttttcaattt tcattaataa ctctgtcgaa gtctgtgctt tatcttgata
      421 attaaatgca gacataccac tttgtataat tgtaattatt gctgaagcaa ttgaaacaat
      481 caatagtaaa gacctgtgtt cggaagcctg atcaattaat attgggatac tagctgataa
      541 gactattttt ataatatttc cgataccaat tactctatta taaaatcgaa tttctttttt
      601 taatctatcg attgtagaat caatagattc gataaacgct ttttgatcca tcattttctc
      661 tccaatcatt ttattaaaat cattaacaaa aatattataa taggatttaa gtaagttcta
      721 actaatcaca cataaaaata aaaaaacatt tgtattttat caaagtttta ttatacttgt
      781 caatttgtgt acatcctaaa atataagcta tttgtttact tctgtagaat atatactttg
      841 ttgttagata agttgcacat gtttatagaa tctaaataat aaatttttat gtaccgcccc
//
LOCUS       DACCKU010000227          892 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.239, whole genome shotgun sequence.
ACCESSION   DACCKU010000227 DACCKU010000000
VERSION     DACCKU010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..892
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.239"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..661)
                     /locus_tag="IVN13_003000"
     CDS             complement(<1..661)
                     /locus_tag="IVN13_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7013418.1"
                     /translation="MSLINVSNLTFSYEGSYDNIFENVSFQIDTDWKLGFIGRNGRGK
                     TTFLNLLLGKYAYSGNISSTVKFEYFPYDVEDKSLYTIEVMKSICTEFMDWEIFREIS
                     LLDVQEDALYRPFNTLSNGEQTKVLLAALFLTASCFLLIDEPTNHLDIDARNVVQNYL
                     KRKKGFILVSHDRSLLDQCVDHILSINKTNIEIQKGNFTSWWENKTLQDNFELAENKK
                     LL"
BASE COUNT          299 a          167 c          118 g          308 t
ORIGIN      
        1 taaggagttt cttgttttct gccagttcaa aattatcttg taacgttttg ttctcccacc
       61 aagaagtaaa atttcccttt tggatttcga tattcgtttt attgatagat agtatatggt
      121 caacacattg atcaagtaag cttctatcat gagataccaa aataaacccc ttcttgcgtt
      181 tcaaatagtt ttgcactaca ttacgtgcat cgatgtcaag atggtttgta ggttcatcaa
      241 taagcaggaa acaactcgct gtaaggaata aagctgcaag aaggaccttc gtttgctcac
      301 catttgacaa tgtattaaac ggacgatata aagcatcttc ttgaacatca agcaatgata
      361 tttcacgaaa aatctcccaa tccataaatt ccgtacaaat actcttcatt acttcaattg
      421 tatatagact cttatcttcc acatcataag gaaaatactc aaacttaact gtagaactta
      481 tattgccgga atacgcatat ttgccaagca gtaaatttaa gaaagtagtt ttaccgcgtc
      541 cgtttcttcc aataaaaccg agtttccaat ctgtatctat ctgaaaactt acattttcaa
      601 aaatattgtc ataacttcct tcatatgaaa aagttagatt tgaaacattt attaaggaca
      661 tatatttctt cctcctttac attataaaaa ttttataagc aaagtcggtt ttcggcatta
      721 attactctat aatgttatcc aactctgcaa ggattttgtc atgaatgaca tagtatcatt
      781 gtctatcacg tttatcttcc tttcttttac aaatattaaa agccgcaaga aagtaaattc
      841 ttacggctca aaaacaaata ctattattac cttttgttat acataacata ag
//
LOCUS       DACCKU010000228          876 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.240, whole genome shotgun sequence.
ACCESSION   DACCKU010000228 DACCKU010000000
VERSION     DACCKU010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.240"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>876
                     /locus_tag="IVN13_003001"
     rRNA            <1..>876
                     /locus_tag="IVN13_003001"
                     /product="23S ribosomal RNA"
BASE COUNT          263 a          179 c          255 g          179 t
ORIGIN      
        1 tcagtcaaag agggtaaaaa ccccgtagac gaaatgtgga agacacctag gaggatcctg
       61 agtacggcgg aacacgagaa attccgtcgg aatccgggag gaccatctcc caaggctaaa
      121 tactccctag tgaccgatag tgaaccagta ccgtgaggga aaggtgaaaa gcaccccgga
      181 aggggagtga aatagaacct gaaaccgtgt gcctacaaca agtcaaagcc cgttaatggg
      241 tgatggcgtg ccttttgtag aatgaaccgg cgagttacga ttgcatgcga ggttaagttg
      301 aagagacgga gccgcagcga aagcgagtct gaatagggcg tttgagtatg tagtcgtaga
      361 cccgaaacca tgtgatctac ccatgtccag gttgaaggtg cggtaaaacg cactggagga
      421 ccgaacccac gtacgttgaa aagtgcgggg atgaggtgtg ggtagcggag aaattccaaa
      481 cgaacttgga gatagctggt tctctccgaa atagctttag ggctagcctc ggaattgaga
      541 atgatggagg tagagcactg tttggactag gggcccatct cgggttaccg aattcagata
      601 aactccgaat gccattcatt catatccggg agtcagactg tgagtgataa gatccatagt
      661 cgaaagggaa acagcccaga ccaccagcta aggtcccaaa atatatgtta agtggaaaag
      721 gatgtggggt tgcacagaca actaggatgt tggcttagaa gcagccacca tttaaagagt
      781 gcgtaatagc tcactagtcg agtgaccctg cgccgaaaat gtaccggggc taaacatatt
      841 accgaagctg tggagtacac ctttaggtgt attggt
//
LOCUS       DACCKU010000229          819 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.241, whole genome shotgun sequence.
ACCESSION   DACCKU010000229 DACCKU010000000
VERSION     DACCKU010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.241"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>819
                     /locus_tag="IVN13_003002"
     CDS             <1..>819
                     /locus_tag="IVN13_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAP7013419.1"
                     /translation="KLDDSPSYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRT
                     RFLCHDCGRTFSAKTDLVDEHHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTR
                     ILREATKHYQSRMNFLPTVLCMDEFKSMKSVSGSMSFICVDGTTNQLFTILEDRRLYK
                     LIQYFLRFPRKARLKVKYLVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQL
                     RVQIMNRFRTSHSENQKKYRRLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTE"
BASE COUNT          251 a          180 c          161 g          227 t
ORIGIN      
        1 aaattggatg actctccttc ctattgtgaa cactgtcgat cggttcgtat tatccgcaac
       61 ggttcctata cgacacgcac tcagatgttg aaagtcaaag aaaagttaac tattttagaa
      121 ttaaaacgta cccgttttct ctgccatgat tgtggaagga ccttttctgc gaaaacggac
      181 ttagttgatg aacatcatca actaacaaag gaactgaagc aggcaattct catggaactg
      241 tacgaaaatc aatcacgtaa actaatcgct aagaagtact tcgtctcgga tgggacggtg
      301 acacggatct tgcgtgaagc aacgaagcat taccagtcac gtatgaactt ccttccgacg
      361 gtgttgtgta tggatgagtt caaatcaatg aagtctgttt ctggttccat gagttttatt
      421 tgtgttgatg ggacaaccaa tcagctattt acgatccttg aagatcgcag actttacaaa
      481 ctaattcaat acttcctgcg ctttcctaga aaggcccgtt taaaggtcaa gtacctagtg
      541 atggacatga atgccagcta ttgtcagttg cttaaaacag tctttccaaa tgcggagatc
      601 gtaactgacc gctttcatat tgtccaacac atcaatcgca gcttcaatca attacgtgtc
      661 cagatcatga atcgttttcg aacctcacac tctgaaaatc agaagaagta ccgtcggctc
      721 aagcgatatt ggaaactctt actcaaagat tccacgacat gggagccact caaacggcat
      781 tatcatcgac tgtttaaacg accaattagt caaacagaa
//
LOCUS       DACCKU010000230          803 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.242, whole genome shotgun sequence.
ACCESSION   DACCKU010000230 DACCKU010000000
VERSION     DACCKU010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.242"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>803
                     /locus_tag="IVN13_003003"
     CDS             <1..>803
                     /locus_tag="IVN13_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HAP7013420.1"
                     /translation="KRQVIDANKHLYPISAMCRILGLSRQSYYYQSKPKKDESELEEV
                     VAEEFIRSRKAYGSRKIKKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTC
                     NEAKTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHK
                     DAVLVKKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNA
                     VAESTYKSLKVEFVYQYTFETLQQLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          286 a          141 c          153 g          223 t
ORIGIN      
        1 gaagagacaa gtaatcgatg cgaataagca tctttaccct atatcagcga tgtgcagaat
       61 attaggtcta tcacgtcagt cctattatta tcaatcaaaa ccaaagaaag acgaatcaga
      121 acttgaagaa gtagtcgctg aagaatttat ccgcagccga aaggcctacg gctcaagaaa
      181 aataaaaaaa gccttatcaa aacgaggcat tcagatcagc cgacgaaaaa ttagtagaat
      241 catgaaaaat agaggattaa aatcgagcta tactgttgct tattttaaag tacatcattc
      301 tacttgcaat gaagccaaaa cgacaaacgt attgaatcgt aaattcttaa gagacaaccc
      361 attagaagcg atcgtaacag acttgactta tgtacgagtc gggaaaaaat ggaattatgt
      421 ctgtttcatt ttggatctgt tcaatcgaga aattctcggc tattcttgtg gagaacataa
      481 agatgccgtt ctagtaaaaa aagcatttag ccgtatcaaa caacctctga cagaggttga
      541 gatttttcat actgatcgtg gaaaagagtt tgataaccaa gctattgatg aattattaac
      601 aacttttgac atcaatcgat cattgagtca taaaggctgt ccttttgata atgccgtagc
      661 tgaatcaact tataagtcgt tgaaagtaga atttgtctat caatacacat ttgaaacctt
      721 acaacaattg gatttggagt tatttgacta tgtcaattgg tggaaccacc ttcggttgca
      781 cggtacactt ggctacgaga cac
//
LOCUS       DACCKU010000231          801 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.243, whole genome shotgun sequence.
ACCESSION   DACCKU010000231 DACCKU010000000
VERSION     DACCKU010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.243"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            129..>801
                     /locus_tag="IVN13_003004"
     CDS             129..>801
                     /locus_tag="IVN13_003004"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7013421.1"
                     /translation="MSMNHFIKQLFTIKDSNIQIQSFEETFYKSRKSIVIEGTLIRTY
                     RRCPCCKDSTKQIVKNGKKISMILLNRSGNKRTYLRLKKQRYLCRACKKYFTARTYLV
                     TPFCFISKQIHYKILEELTERQSIKAIGKHCDVSVTTVQRTLSALSSDIRQNLEYLPR
                     CLLFDEFRSLSTWHGKFSFSCMDGETGKLFEILPSRRKKDLVAFFMRFSLKARLGVHY
                     IVTDMN"
BASE COUNT          240 a          153 c          147 g          261 t
ORIGIN      
        1 gctctataat ttctaggact gatggaaatt ttctatctgt cctagttttt ttgtaaaaaa
       61 acaaaaagac atacaaaatt tccggtagaa tagagtctcc tacaacatca ttctggggga
      121 attcttgtat gtccatgaat cattttatca aacaactctt tactattaaa gactcaaata
      181 tccaaattca atcctttgaa gaaacttttt ataaaagtag aaaaagtatc gttattgagg
      241 gaactctgat ccgtacctac agacgttgtc cgtgttgtaa agattctaca aagcaaatcg
      301 ttaaaaatgg caagaagatc tctatgattc tcctgaatcg tagcggtaat aaacggacct
      361 atcttcggtt gaagaaacag cgatacctct gccgagcatg taaaaaatat tttaccgctc
      421 ggacgtatct cgttacccca ttctgcttta tttctaagca gattcattac aagatcttag
      481 aagaattgac agagcgtcaa tctataaaag caattggtaa acattgcgat gtttctgtga
      541 caactgttca gcggacattg tccgccttgt catcagatat ccgtcagaat cttgagtatt
      601 tacctcggtg tctattattc gatgagtttc gttctctttc tacatggcac ggtaaatttt
      661 ctttttcttg tatggacggt gagaccggga aactgtttga gatcttacct agccgccgta
      721 aaaaagatct cgttgctttt ttcatgcgtt tttctctcaa agcgaggttg ggtgtacatt
      781 acatcgtaac ggatatgaac g
//
LOCUS       DACCKU010000232          797 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.244, whole genome shotgun sequence.
ACCESSION   DACCKU010000232 DACCKU010000000
VERSION     DACCKU010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.244"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>797
                     /locus_tag="IVN13_003005"
     CDS             <1..>797
                     /locus_tag="IVN13_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7013422.1"
                     /translation="LADGQKGDNRLKTLKEIQQIYPQIPESKGGFPGYGGIPITHYKK
                     YWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAERQSSMVVNDPKTELYVASKDE
                     LEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDIDTATQLTNTFTNTIYYDANAS
                     DNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNNLVELLSEIGGFNYQDPDNEFK
                     QLNALNEFMNQLPPGNIAKKQYGATKIGSDKAKGNILSTAITGLNPFT"
BASE COUNT          291 a          137 c          157 g          212 t
ORIGIN      
        1 ctagcagatg ggcaaaaagg agataaccga ttaaaaacgt taaaggaaat ccaacaaatt
       61 tatccgcaaa taccagaatc aaagggaggt tttcctggtt atggcggaat accaattact
      121 cattataaaa agtactggta tattcagacg gatacagtaa atacttgtat tgttggtact
      181 tcccgttcag ggaaaggaca gatagaagtt ttagcaacca ttgacaattt gtctcgtgca
      241 gaaagacaaa gttcaatggt tgttaatgat ccaaaaacag aattatatgt tgctagtaaa
      301 gatgaactag aagctagagg gtatgatgtg tatgcattta atatcaccga tccactgcaa
      361 tcaatgagtg ataatccatt ggcactcatc gtgaaatact ggaagcgagg agacatagac
      421 acagcgacac agttaaccaa tactttcacg aatacgattt attatgatgc caacgcttca
      481 gacaataaat actttaacga aaatgcgcaa aaagctgtca atgcgcttat ttttgcgtta
      541 ttggaacaag cggatcagac aggggattac tccaaattaa caccgaataa tctcgtcgaa
      601 cttctttctg agattggtgg ttttaattac caagatcctg ataatgaatt caagcagctc
      661 aatgctttaa atgaatttat gaatcagtta ccacctggaa acatagcaaa aaaacaatat
      721 ggggcaacaa aaattggatc tgataaagcg aaagggaata tcttgtctac ggcaattaca
      781 ggattgaatc catttac
//
LOCUS       DACCKU010000233          789 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.247, whole genome shotgun sequence.
ACCESSION   DACCKU010000233 DACCKU010000000
VERSION     DACCKU010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.247"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>789
                     /locus_tag="IVN13_003006"
     rRNA            <1..>789
                     /locus_tag="IVN13_003006"
                     /product="23S ribosomal RNA"
BASE COUNT          196 a          172 c          251 g          170 t
ORIGIN      
        1 catgtacagg ataggtagga gccgtagaaa tcggaacgct agtttcgatg gaggcgctgg
       61 tgggatacta cccctgcgtt atggccactc taacccgcac cactaatcgt ggtgggagac
      121 agtgtcagat gggcagtttg actggggcgg tcgcctccta aaaggtaacg gaggcgccca
      181 aaggttccct cagaatggtt ggaaatcatt cgaagagtgt aaaggcagaa gggagcttga
      241 ctgcgagacc aacaagtcga gcagggacga aagtcgggct tagtgatccg gtggttccgc
      301 atggaagggc catcgctcaa cggataaaag ctaccctggg gataacaggc ttatctcccc
      361 caagagtcca catcgacggg gaggtttggc acctcgatgt cggctcgtcg catcctgggg
      421 ctgtagtcgg tcccaagggt tgggctgttc gcccattaaa gcggcacgcg agctgggttc
      481 agaacgtcgt gagacagttc ggtccctatc cgtcgcgggc gttggaaatt tgagaggagc
      541 tgtccttagt acgagaggac cgggatggac ttaccgctgg tgtaccagtt gttctgccaa
      601 gggcattgct gggtagctat gtagggaagg gataaacgct gaaagcatct aagtgtgaag
      661 cccacctcaa gatgagattt cccatttctt taagaaagta agatccctga gagatgatca
      721 ggtagatagg tcaggagtgg aagtacagtg atgtatggag cggactgata ctaatcgatc
      781 gaggactta
//
LOCUS       DACCKU010000234          768 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.248, whole genome shotgun sequence.
ACCESSION   DACCKU010000234 DACCKU010000000
VERSION     DACCKU010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.248"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            27..>768
                     /locus_tag="IVN13_003007"
     CDS             27..>768
                     /locus_tag="IVN13_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP7013423.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADP"
BASE COUNT          268 a          134 c          144 g          222 t
ORIGIN      
        1 caaagaatta taaggagtga gtacaaatga cctataccca tcttacaacg gatgaacttg
       61 taataataga gtcttatttc aaaatgaatc aatctgttgc taaaactgcc cattgcttga
      121 atcgttcaag acaaacgatc cataaagtat atctgttctt caagcaagga aaatcagcct
      181 tagaatatta tcaacagtat aagaaaaaca aatcaaactg tggtagacgt ccgcttgttt
      241 tacccgagga acaatcagaa tatattcaaa gaaaggttgt tcaaggatgg acacccgatg
      301 tgattgttgg tcgtgcagcg tttcctatta gttgttctgc tcgtaccatt tatcgtatgt
      361 tcaaaaaggg gctatttgat tcttctgact taccgatgaa aggcaagcgt aaaccgaatg
      421 gacatcaaga aagacgtgga aaacaaactt tccgccgctc tattcatgaa cgtgaaaagg
      481 attatagcca attctcaaat gagtttggtc accttgaagg tgacactatc gtaggtctga
      541 aacataaaag tgctgtaatt accttagttg aacgattatc aaaagttatc atcacattga
      601 aaccgtgtgg tagacaagcg attgatattg aaaaaaaatt aaatcaatgg tttgaatctg
      661 taccgaaaaa cctattcaaa tccatcactt ttgattgtgg aaaggaattt tcaaattgga
      721 aacagatcag taatgtcaat gatattgcca tttatttcgc tgatccag
//
LOCUS       DACCKU010000235          749 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.249, whole genome shotgun sequence.
ACCESSION   DACCKU010000235 DACCKU010000000
VERSION     DACCKU010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.249"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..365
                     /locus_tag="IVN13_003008"
     CDS             <1..365
                     /locus_tag="IVN13_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013424.1"
                     /translation="RKTAEEFGQLAIDKSLSRNELTEKLKDVATQEVIDKLSPVDDDH
                     QHDDYGSYSFTLGDVQTYAQTSSVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVT
                     EKQVDGQWKVTDFSYFTR"
     gene            376..>749
                     /locus_tag="IVN13_003009"
     CDS             376..>749
                     /locus_tag="IVN13_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013425.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLA"
BASE COUNT          292 a          111 c          157 g          189 t
ORIGIN      
        1 aaagaaagac ggcagaagag ttcggtcaat tggccattga taaatcatta agcagaaatg
       61 aattaacgga aaaactaaaa gatgtcgcaa cccaagaagt gatcgacaaa ttaagtcctg
      121 tagacgatga tcaccaacat gatgattacg gaagttactc ttttacatta ggtgacgtgc
      181 aaacttacgc tcaaacaagc agtgtaaaag aggaacaaaa ttttgtcgta tttgttgatt
      241 atacaatcgg caatcctcaa ttcaaagatg tcaaaccaca aaaaataaaa ggtggattaa
      301 ctgtcactga aaaacaagta gatggtcaat ggaaagtcac agactttagt tatttcactc
      361 gttaggagga agaaaatgag taagaaaaaa ggagcaagac aacgttttaa aaaacgaaaa
      421 agttatctta gatggctggg gatctttatg attctcggct ttttgattct tgctagtccg
      481 atttacacac ataaaaaggt agacatacca gatgaagtaa gcacgaacgc atttttaaac
      541 acgaaggaac tgacgatggt ttcaaaagaa aaaggagcga accagcaatt agtttttgaa
      601 tttgcgatcg acgatgaaga tcagagctta ttaaaagaac tagctaatct taattatcgt
      661 gtagaagtaa agacagctaa aggggattat caagcaataa aagccaaagt gatcaaggtg
      721 tctgatgatt attttgtagt gaagttagc
//
LOCUS       DACCKU010000236          739 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.250, whole genome shotgun sequence.
ACCESSION   DACCKU010000236 DACCKU010000000
VERSION     DACCKU010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.250"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(266..466)
                     /locus_tag="IVN13_003010"
     CDS             complement(266..466)
                     /locus_tag="IVN13_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP7013426.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
BASE COUNT          265 a          118 c          126 g          230 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccatcaataa acagtcgaag agaaaatagg gaaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caatagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatcca acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgcttta
//
LOCUS       DACCKU010000237          731 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.251, whole genome shotgun sequence.
ACCESSION   DACCKU010000237 DACCKU010000000
VERSION     DACCKU010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.251"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            97..627
                     /locus_tag="IVN13_003011"
     CDS             97..627
                     /locus_tag="IVN13_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013427.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(622..>731)
                     /locus_tag="IVN13_003012"
     CDS             complement(622..>731)
                     /locus_tag="IVN13_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013428.1"
                     /translation="NIVKDLNEGVSISKIAKNYNVTRQTIYRIKKDLNL"
BASE COUNT          293 a           86 c          111 g          241 t
ORIGIN      
        1 ttctaacatc agttattatg ataattttga tgatgtcaat tatgatttac aggaaagata
       61 taatgattta atcgaagaat tagaggagag aggagaatga tttcagataa taaatgggaa
      121 ataaatctca aaagatttat ttcagaatat atatatacga ttgaaactaa atctagtttt
      181 tctcttaatg aagacagtga tcctctaaaa aaatacacta ttcaatgtaa gaaaaataaa
      241 gatgaaaaat tgttcgtaat aagaccagat cttatgaaga gtaataccca ctatataaaa
      301 gaaaaaccta aagattgtga ctacatcatc ataagtgaag caaataaaac agtatttctg
      361 atagaactta aaagctcagc tcagacttcc cctgcaaaag aaattagtga acaaattgat
      421 ggtggatata agtggttaca acatcttagt tttctaacaa aatatgattt tactgaattc
      481 agttataaaa ttattaaggt atgttgtaaa tttgaggcaa gacaagatcg taaaaatctt
      541 tttaaaccta atgaatatgg tatttataat atgaatggtc caaaattaga cttaagagag
      601 ttgtatagaa agtgtaacga tctataaatt caaatctttt ttaattctat atattgtttg
      661 tctagtaacg ttataattct ttgcaatctt tgaaatagag acgccctcat tcaagtcctt
      721 aacgatgttt t
//
LOCUS       DACCKU010000238          731 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.252, whole genome shotgun sequence.
ACCESSION   DACCKU010000238 DACCKU010000000
VERSION     DACCKU010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.252"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVN13_003013"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="IVN13_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP7013429.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="IVN13_003014"
     CDS             complement(481..675)
                     /locus_tag="IVN13_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013430.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DACCKU010000239          714 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.253, whole genome shotgun sequence.
ACCESSION   DACCKU010000239 DACCKU010000000
VERSION     DACCKU010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.253"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>714
                     /locus_tag="IVN13_003015"
     CDS             <1..>714
                     /locus_tag="IVN13_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HAP7013431.1"
                     /translation="KSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPYLMTDVLYIKV
                     REENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKERGLQGTELVI
                     SDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGIFKFTDINL
                     AREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLIERLNQEV
                     RRREKIIRIFPNQTSANRL"
BASE COUNT          260 a          141 c          124 g          189 t
ORIGIN      
        1 taaatccgtc tctaagtcct tcgtttctag cttaacagaa cagctagaac ctatggttaa
       61 cgagtggcag aatcgtttat tatcagaaaa aaattatcct tacttaatga ccgatgtact
      121 ctatataaaa gtacgagaag aaaatcgagt actctcaaaa agctgtcata tagcgattgg
      181 aataaccaaa gatggcgacc gtgaaattat cggcttcatg attcaaagtg gcgaaagcga
      241 agagacctgg acaacatttt ttgaatacct aaaagaacgc ggtttacaag gtacggaact
      301 cgttatttct gatgcgcaca aaggattagt ctctgccatt agaaaatcct tcaccaacgt
      361 aagttggcaa agatgccaag ttcacttcct aagaaatatc tttaccacca ttcctaaaaa
      421 aaattcaaaa tctttcagag aagctgttaa aggaattttt aagttcacag atattaactt
      481 agcgcgtgag gctaaaaatc gattgattca tgattatatc gatcaaccaa aatattcaaa
      541 agcttgcgca tcattggatg atggattcga agacgccttt caatataccg tacaaggaaa
      601 ttcccacaat cgactaaaga gtaccaatct aattgaacga ctgaatcaag aagtacgcag
      661 aagagaaaag attattcgca tcttccccaa tcaaacatca gccaatcgct taat
//
LOCUS       DACCKU010000240          690 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.254, whole genome shotgun sequence.
ACCESSION   DACCKU010000240 DACCKU010000000
VERSION     DACCKU010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.254"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..549
                     /gene="tnpB"
                     /locus_tag="IVN13_003016"
     CDS             <1..549
                     /gene="tnpB"
                     /locus_tag="IVN13_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP7013432.1"
                     /translation="IDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQ
                     KRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSW
                     SKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHD
                     RDINASINILTEGLRLHSMGLA"
BASE COUNT          256 a          111 c          132 g          191 t
ORIGIN      
        1 attgataata ataaattcac gtccaaaatg gaaaagaaac taaagcgtga acaacgcaaa
       61 ttgtctaggc gtgctttatt ggctaaaaat aaaggtacca atctatttga agctaaaaat
      121 tatcaaaaac aaaaacgcaa agtagctaga ttgcatgaaa aagtaatgaa tcaacgtact
      181 gattttctga ataagttgag tacagaaatg atcaaaaatc acgatattat ctgtattgaa
      241 gacttaaaca caaaaggtat gttgcgaaat cataaattag ctaaaagtat ttctgatgtt
      301 tcttggtcaa agtttgtgac taaattacaa tacaaggctg actggtatgg acgaaaaatc
      361 atcaaagtag ataaatggtt tccgtctagt cagatttgtt cagagtgcgg acataaagat
      421 ggcaagaaat ctcttgaaat tcgagaatgg acttgtcctg tttgtcatac tcatcatgat
      481 cgtgatatca atgctagtat caatattctg accgaaggtc taagactaca ttctatggga
      541 ttggcttaga tccaatcatc aagaaccgta ggaactacgg ggatagcttg gtaaataaga
      601 gacaccgctg ttagtaaaga aatacgctat caagtatgct ctattcccaa gaagctccca
      661 cttcaagcgt taagaaccgt caggtttagc
//
LOCUS       DACCKU010000241          669 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.255, whole genome shotgun sequence.
ACCESSION   DACCKU010000241 DACCKU010000000
VERSION     DACCKU010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.255"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..156)
                     /locus_tag="IVN13_003017"
     CDS             complement(<1..156)
                     /locus_tag="IVN13_003017"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013433.1"
                     /translation="MEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQP
                     VTAKKETV"
BASE COUNT          213 a          102 c          113 g          241 t
ORIGIN      
        1 tactgtttct ttttttgcag ttacaggttg tttattttcg gaaaaagcta atatagctaa
       61 gattgaaaca tccatctgga atttatcttt tttactgctt tgatcataaa agtctgcata
      121 ttcatcactg atattttttc taataaattc ttccatcata aaaatcacct caaaacgatt
      181 atacgaactt acgttcttgt tgtaaagcga tatttgaggg gcaaaaaagg ggcaaaaaac
      241 tcttactagt ccgttctagc ctaaaaaatt caaacattgc caacgttgct tttccttgat
      301 ttgatgggat ttttgtccag tgtggacaag tgaaaaacat ttgtcttgat gggcggtatg
      361 atgtaagaaa aagcaacttg cttatctaca actgatcatt agtgaaagac agataaaaag
      421 aggctggaac agaagcgttt aactccaaga aataagaaga aattcacgga aattgctttt
      481 caaatttttg tgaatttcag cttatttccg aaggagttgc ttctgcttcc gctgtttata
      541 cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt cccacgctct tttttgtgtg
      601 taattataat gaaaatagtt atttttaata gttataatat tttatctatt aaaagtaaat
      661 atccattca
//
LOCUS       DACCKU010000242          664 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.256, whole genome shotgun sequence.
ACCESSION   DACCKU010000242 DACCKU010000000
VERSION     DACCKU010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.256"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            15..>664
                     /locus_tag="IVN13_003018"
     CDS             15..>664
                     /locus_tag="IVN13_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982-like element ISEfm1 family transposase"
                     /protein_id="HAP7013434.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LKQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIK
                     IIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLK
                     IHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLAL"
BASE COUNT          234 a          120 c          112 g          198 t
ORIGIN      
        1 aatgaggact acatatgcgt tcatcaaaat atactgaaca ttatacagat acttttataa
       61 cattcaagga aattatgaga actgtttcta acatatacca caattgtgta ccagataaga
      121 ttaaaaaccg aagaaatact gatcaactga agcaacgcga tacagtgatt attgcttgtg
      181 ttatatgggg cataattaat ggctatacta gccaaagagc cacgtataga gcggtttgtt
      241 ccgtcttatt tcctaatggt gactttccta gtaggagtcg attcactcgc ttaagttcaa
      301 acttagctta cactatcaag attattcgat actttttcat caagaagctc accaaaggtg
      361 aactagtcgg aattatagat agttttccta gtccattatg taaaccagtt agaaatagac
      421 aagcaaaact attgaatcaa atagccaaag ttggctataa ctcaacaaaa aaatcttatt
      481 tttatggtct taaaattcat atgatcgtta ctaagactgg ctttccgatt acttactcaa
      541 tcacgaatcc aggtgtccat gacgtgaaag tgttagagac tttatcggaa gaagcgaatc
      601 ttcccaatat cctaggggac aaaggatata ttagccataa aatccatgaa aagcttgccc
      661 ttaa
//
LOCUS       DACCKU010000243          652 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.257, whole genome shotgun sequence.
ACCESSION   DACCKU010000243 DACCKU010000000
VERSION     DACCKU010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.257"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..647
                     /locus_tag="IVN13_003019"
     CDS             <1..647
                     /locus_tag="IVN13_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013435.1"
                     /translation="KWKKEYEIQQQELKEQEEKQRQEEKEREQLELKKREQHLAEQKV
                     AIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQANLTPS
                     PQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQEQARKEY
                     EQQLEELLNRTNQSTAESSMENSKESDPSKAKEGSSDSTVHSNTSESSESNEVK"
BASE COUNT          272 a          117 c          139 g          124 t
ORIGIN      
        1 caaaatggaa aaaagagtat gaaattcaac aacaagaatt gaaagaacaa gaagaaaaac
       61 aacgacaaga agaaaaagag cgcgaacaat tagaactaaa aaaacgtgag cagcatcttg
      121 cagaacaaaa agtggcgata gaaaaacaac ggttagagca agaaaaggaa aaagaagcag
      181 ctgctcgtaa ggaacgtcaa gaacaaatgg atacattgcg acaagcacag ttgaattatc
      241 tagcacaacc tcaaccagta gctgctgttc aaacgccagt gaacaaccaa gcaaatttaa
      301 ctccttcacc acagcgttcc tttaacggat ggatggtggg atgtattgca agtttaagct
      361 tgcttctagg tggaggaaca gtatttgctt ttaatcatat caatcaatct tcacaggcag
      421 attctgtagc cacaacggcg tcaatcaagg aagaagccca ggaacaagca agaaaagagt
      481 atgaacaaca attggaagaa ttattgaatc gtaccaatca gtcaacagca gagtcaagta
      541 tggaaaatag caaagagagt gatcctagta aggctaaaga aggatcaagc gattcaactg
      601 tacactcaaa cacttccgaa agttcagaat caaatgaggt gaaataatgc gt
//
LOCUS       DACCKU010000244          635 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.259, whole genome shotgun sequence.
ACCESSION   DACCKU010000244 DACCKU010000000
VERSION     DACCKU010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.259"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>635
                     /gene="erm"
                     /locus_tag="IVN13_003020"
     CDS             <1..>635
                     /gene="erm"
                     /locus_tag="IVN13_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HAP7013436.1"
                     /translation="SQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKIS
                     KQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQI
                     IKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPK
                     VNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA"
BASE COUNT          234 a          122 c          100 g          179 t
ORIGIN      
        1 attctcaaaa ctttttaacg agtgaaaaag tactcaacca aataataaaa caattgaatt
       61 taaaagaaac cgataccgtt tacgaaattg gaacaggtaa agggcattta acgacgaaac
      121 tggctaaaat aagtaaacag gtaacgtcta ttgaattaga cagtcatcta ttcaacttat
      181 cgtcagaaaa attaaaactg aatactcgtg tcactttaat tcaccaagat attctacagt
      241 ttcaattccc taacaaacag aggtataaaa ttgttgggaa tattccttac catttaagca
      301 cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc tgacatctat ctgattgttg
      361 aagaaggatt ctacaagcgt accttggata ttcaccgaac actagggttg ctcttgcaca
      421 ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga atgctttcat cctaaaccaa
      481 aagtaaacag tgtcttaata aaacttaccc gccataccac agatgttcca gataaatatt
      541 ggaagctata tacgtacttt gtttcaaaat gggtcaatcg agaatatcgt caactgttta
      601 ctaaaaatca gtttcatcaa gcaatgaaac acgcc
//
LOCUS       DACCKU010000245          616 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.260, whole genome shotgun sequence.
ACCESSION   DACCKU010000245 DACCKU010000000
VERSION     DACCKU010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.260"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            51..>616
                     /locus_tag="IVN13_003021"
     CDS             51..>616
                     /locus_tag="IVN13_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP7013437.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          223 a          103 c          120 g          170 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccac
//
LOCUS       DACCKU010000246          606 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.261, whole genome shotgun sequence.
ACCESSION   DACCKU010000246 DACCKU010000000
VERSION     DACCKU010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.261"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            166..372
                     /locus_tag="IVN13_003022"
     CDS             166..372
                     /locus_tag="IVN13_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013438.1"
                     /translation="MVKFPQFKLNPAASIQFQGHVFDVLAKDNIKKYKKGNIFHIDIQ
                     ISSNLEDIKKAFKIKTIGTAPNFK"
BASE COUNT          261 a           76 c           68 g          201 t
ORIGIN      
        1 gaattatatg tttttaatag tttagatgtt gaaataccta tgaaacagat gaaaaatatt
       61 gacatacata tactgccaag tgagtcgtgc caaatagaat attcaatcga atatttttac
      121 aaaggtaaaa aaataaaaaa atgtgacaag tgttttgaat cagctgtggt aaagtttcct
      181 caatttaaac taaatccagc tgcctcgata caatttcaag gacatgtatt tgatgtttta
      241 gctaaagata acataaaaaa atacaaaaaa ggtaatatat tccatattga tatacaaata
      301 tcttctaatt tagaagatat aaaaaaagcc tttaaaatta aaacaattgg cactgctcct
      361 aattttaaat aacaaaaaaa ttactagata atctaaaaac aggatggcat tatgagaaca
      421 ataactaaac ttcctatatc acatattttg taattagtga aaagcaaatt gactactatc
      481 ataagttaat gactctcatc ttaatattta aaatatattc atatttttct attttatcta
      541 aatgcgattt tcatatctaa taatagaaaa ataaacaatt aacaataaaa aagtcttcat
      601 ttattg
//
LOCUS       DACCKU010000247          588 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.262, whole genome shotgun sequence.
ACCESSION   DACCKU010000247 DACCKU010000000
VERSION     DACCKU010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.262"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..>588)
                     /locus_tag="IVN13_003023"
     rRNA            complement(<1..>588)
                     /locus_tag="IVN13_003023"
                     /product="16S ribosomal RNA"
BASE COUNT          116 a          195 c          124 g          153 t
ORIGIN      
        1 atcctgtttg ctccccacgc tttcgagcct cagcgtcagt tacagaccag agagccgcct
       61 tcgccactgg tgttcctcca tatatctacg catttcaccg ctacacatgg aattccactc
      121 tcctcttctg cactcaagtc tcccagtttc caatgaccct ccccggttga gccgggggct
      181 ttcacatcag acttaagaaa ccgcctgcgc tcgctttacg cccaataaat ccggacaacg
      241 cttgccacct acgtattacc gcggctgctg gcacgtagtt agccgtggct ttctggttag
      301 ataccgtcaa gggatgaaca gttactctca tccttgttct tctctaacaa cagagtttta
      361 cgatccgaaa accttcttca ctcacgcggc gttgctcggt cagactttcg tccattgccg
      421 aagattccct actgctgcct cccgtaggag tttgggccgt gtctcagtcc caatgtggcc
      481 gatcaccctc tcaggtcggc tatgcatcgt ggccttggtg agccgttacc tcaccaacta
      541 gctaatgcac cgcgggtcca tccatcagcg acacccgaaa gcgccttt
//
LOCUS       DACCKU010000248          585 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.263, whole genome shotgun sequence.
ACCESSION   DACCKU010000248 DACCKU010000000
VERSION     DACCKU010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.263"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>585
                     /locus_tag="IVN13_003024"
     CDS             <1..>585
                     /locus_tag="IVN13_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAP7013439.1"
                     /translation="KPDKYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTV
                     RKLMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGR
                     KIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQM
                     PQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVL"
BASE COUNT          253 a           79 c           98 g          155 t
ORIGIN      
        1 taagccagat aaatatagca agataaagca agaaattaca gcgattgtaa aagaatcaag
       61 aaactcttat ggttatcgta gagtcacttt agcgttaaag atgaaaggat atacaatcaa
      121 tcataaaaca gttagaaaat taatgtccca aatgggactt acctgtcaaa tccgaataaa
      181 gcgatataaa tcttataaag gaacagtagg aaaaattgcc aagaatgtgt taaaacgaaa
      241 tttttcagta gatacaccca ataaaaaatg ggtgacagac gtcactgaat tcaagataaa
      301 aggaagaaaa atctatctat ctcctatact tgatttattt aacggagaaa taattagtta
      361 tagtatatca actagtccaa catataaact gattgaagaa atgcttcaac aagcaattaa
      421 aaaaaaggga actgagggtt cactgattct acattcagat caaggatggc agtatcagat
      481 gccacaatat caaaaaaaat taaaagagaa taacattatt caaagcatgt ctagaaaagg
      541 aaattgctta gataattctg taatagaaaa tttttttggt gtgct
//
LOCUS       DACCKU010000249          572 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.264, whole genome shotgun sequence.
ACCESSION   DACCKU010000249 DACCKU010000000
VERSION     DACCKU010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.264"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            33..>572
                     /locus_tag="IVN13_003025"
     CDS             33..>572
                     /locus_tag="IVN13_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP7013440.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIY"
BASE COUNT          211 a          109 c           92 g          160 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa
      541 aacgtcaaac cgtatccaaa gaagcgattt at
//
LOCUS       DACCKU010000250          549 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.265, whole genome shotgun sequence.
ACCESSION   DACCKU010000250 DACCKU010000000
VERSION     DACCKU010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.265"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(76..165)
                     /locus_tag="IVN13_003026"
     tRNA            complement(76..165)
                     /locus_tag="IVN13_003026"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(127..129),aa:Ser,seq:tga)
     gene            complement(189..262)
                     /locus_tag="IVN13_003027"
     tRNA            complement(189..262)
                     /locus_tag="IVN13_003027"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(226..228),aa:Ile,seq:cat)
     gene            complement(276..349)
                     /locus_tag="IVN13_003028"
     tRNA            complement(276..349)
                     /locus_tag="IVN13_003028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(313..315),aa:Met,seq:cat)
     gene            complement(377..449)
                     /locus_tag="IVN13_003029"
     tRNA            complement(377..449)
                     /locus_tag="IVN13_003029"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(414..416),aa:Ala,seq:tgc)
     gene            complement(469..542)
                     /locus_tag="IVN13_003030"
     tRNA            complement(469..542)
                     /locus_tag="IVN13_003030"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(506..508),aa:Pro,seq:tgg)
BASE COUNT          144 a          155 c          130 g          120 t
ORIGIN      
        1 ggatttgaac ctacgacctt cgggttatga gcccgacgag ctaccagact gctccatccc
       61 gcgataatat gaaaaaggag gataagggat tcgaaccctt gcacggtttt acccgcctga
      121 cggttttcaa gaccgttccc ttcagccgga cttgggtaat cctccatatg aaacttacaa
      181 cgccacaatg gaccttgtag gactcgaacc tacgaccgga cggttatgag ccgtctgctc
      241 taaccaactg agctaaaggt ccaggctctt aaaaatcgcg gcggagggga tcgaaccccc
      301 gacctcccgg gtatgaaccg gacgctctag ccagctgagc tacaccgcga aaaagatatt
      361 cattatttaa atgaaatgga gcctagcggg atcgaaccgc tgacctcctg cgtgcaaagc
      421 aggcgctctc ccagctgagc taaggccccg tgtttaaatt tataattatc gggaagacag
      481 gattcgaacc tgcgacccct tggtcccaaa ccaagtgctc taccaagctg agctacttcc
      541 cgtttaatt
//
LOCUS       DACCKU010000251          543 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.266, whole genome shotgun sequence.
ACCESSION   DACCKU010000251 DACCKU010000000
VERSION     DACCKU010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.266"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
BASE COUNT          209 a           87 c           52 g          195 t
ORIGIN      
        1 atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg caaccaatag
       61 aaaaaaatac ttagaacggt agatcatcgc taataacaat gcattgtcca ccaaatggat
      121 tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag gcctctcccc
      181 ttctattata aaagaagata tatattattt cttaattacc tactacctta aaccaaaatt
      241 taagtactac ctaatttaag tagtacttaa ataatctaaa aaatagttat cccaattgtt
      301 atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa acactaccat
      361 atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc tcttaacttc
      421 aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc tagacaaact
      481 acaagcatgt agttttatct gttaattatg tactacctaa ataaattagt ttataattaa
      541 caa
//
LOCUS       DACCKU010000252          530 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.267, whole genome shotgun sequence.
ACCESSION   DACCKU010000252 DACCKU010000000
VERSION     DACCKU010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.267"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(7..92)
                     /locus_tag="IVN13_003031"
     tRNA            complement(7..92)
                     /locus_tag="IVN13_003031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Leu,seq:taa)
     gene            complement(109..180)
                     /locus_tag="IVN13_003032"
     tRNA            complement(109..180)
                     /locus_tag="IVN13_003032"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(146..148),aa:Gly,seq:gcc)
     gene            complement(192..264)
                     /locus_tag="IVN13_003033"
     tRNA            complement(192..264)
                     /locus_tag="IVN13_003033"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(229..231),aa:Thr,seq:tgt)
     gene            complement(282..363)
                     /locus_tag="IVN13_003034"
     tRNA            complement(282..363)
                     /locus_tag="IVN13_003034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(327..329),aa:Leu,seq:tag)
     gene            complement(371..443)
                     /locus_tag="IVN13_003035"
     tRNA            complement(371..443)
                     /locus_tag="IVN13_003035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(408..410),aa:Lys,seq:ttt)
     gene            complement(447..519)
                     /locus_tag="IVN13_003036"
     tRNA            complement(447..519)
                     /locus_tag="IVN13_003036"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(484..486),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          115 t
ORIGIN      
        1 ttgaaatgcc gaggaccgga atcgaaccgg tacggtgatc actcaccgca ggattttaag
       61 tcctgtgcgt ctgccagttc cgccaccccg gcgcgattgc tttggcaaag cggaaaacgg
      121 ggttcgaacc cgcgaccccc accttggcaa ggtggtgctc taccactgag ctatttccgc
      181 gtgtttaaag atgccggcta aaggacttga accctcgacc ctctgattac aaatcagatg
      241 ctctaccaac tgagctaagc cggcatgtaa aacttaaatt atgcgggtga agggacttga
      301 acccccacgc cgtaaggcgc tagatcctaa atctagtgcg tctgccaatt ccgccacacc
      361 cgcaaaaata tgagccgtac agggctcgaa cctgtgaccc tctgattaaa agtcagatgc
      421 tctaccaact gagctaacgg ctcatatgga ggttaacggg atcgaaccgc tgaccctctg
      481 cttgtaaggc agatgctctc ccagctgagc taaacctcca tggataactt
//
LOCUS       DACCKU010000253          515 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.268, whole genome shotgun sequence.
ACCESSION   DACCKU010000253 DACCKU010000000
VERSION     DACCKU010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.268"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>515
                     /locus_tag="IVN13_003037"
     CDS             <1..>515
                     /locus_tag="IVN13_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP7013441.1"
                     /translation="KLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRG
                     LGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQ
                     DILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLEC
                     LNNHIKVLKRNA"
BASE COUNT          177 a          108 c           98 g          132 t
ORIGIN      
        1 aagcttttcc cgaatgctca gttgatcatc gatcgtttcc acatcgtcca acatattggc
       61 agaactttct tgaatcatcg agtgaaagaa accacggtac gacttaaagg aaattcccac
      121 tctcaacgag ggttagggaa aaaattgaag cgctattgga aactcctaca aaaagaagaa
      181 gcaaagttaa actatgaaaa acgggtttgg cgtgccagtt ttaaagatta tttgactgaa
      241 agtgagattg ccgatcgact gttagctgct tgtcccaact tacgccaagg ctatcagtta
      301 taccaagaca ttctttatgc ggtaaagaaa agagatcagt cactatttga ggattgttta
      361 actagagaca ttagcggtct gccagaaacc tatacaacca ctttacgaac gtttaaaaag
      421 tttctgccac agatccaaaa tgcgcttcac tacagctatt caaacggacc attggaatgc
      481 ttaaacaacc acatcaaagt attaaaacgc aatgc
//
LOCUS       DACCKU010000254          509 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.269, whole genome shotgun sequence.
ACCESSION   DACCKU010000254 DACCKU010000000
VERSION     DACCKU010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.269"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IVN13_003038"
     CDS             complement(<1..132)
                     /locus_tag="IVN13_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP7013442.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACCKU010000255          505 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.270, whole genome shotgun sequence.
ACCESSION   DACCKU010000255 DACCKU010000000
VERSION     DACCKU010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.270"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>505
                     /locus_tag="IVN13_003039"
     rRNA            <1..>505
                     /locus_tag="IVN13_003039"
                     /product="23S ribosomal RNA"
BASE COUNT          154 a          113 c          144 g           94 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagac
//
LOCUS       DACCKU010000256          493 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.271, whole genome shotgun sequence.
ACCESSION   DACCKU010000256 DACCKU010000000
VERSION     DACCKU010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.271"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            56..>493
                     /locus_tag="IVN13_003040"
     CDS             56..>493
                     /locus_tag="IVN13_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP7013443.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRV"
BASE COUNT          151 a           98 c           93 g          151 t
ORIGIN      
        1 acaccctgaa atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc
       61 tcatactcag cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga
      121 aaattgttta actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct
      181 tctgtacaag ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg
      241 ttgggggacg acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct
      301 ggagctgaag aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac
      361 ctctgttgct gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg
      421 cttaaaagag aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc
      481 ggtctatcgt gtc
//
LOCUS       DACCKU010000257          484 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.272, whole genome shotgun sequence.
ACCESSION   DACCKU010000257 DACCKU010000000
VERSION     DACCKU010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.272"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..245
                     /locus_tag="IVN13_003041"
     CDS             <1..245
                     /locus_tag="IVN13_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP7013444.1"
                     /translation="FVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELK
                     DEENKKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            261..>484
                     /locus_tag="IVN13_003042"
     CDS             261..>484
                     /locus_tag="IVN13_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013445.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIV"
BASE COUNT          179 a           90 c           92 g          123 t
ORIGIN      
        1 actttgtcac ttacttagaa ccaaatccca atgaagatcg agtaacgcta atgacatgta
       61 cgcctaaggg gatcaacaca caccgtttcc tagtgtatgg gaagcgagta acctttacaa
      121 aatcagaatt aaaagatgaa gaaaataaga aacaaaaatt atcttggaaa tggcttttag
      181 gaagtactgt ttttctttca gtcatgatca ttggttcgct atttgtttat aaaaagaaaa
      241 aataaaggag ggtaaccaac atgagaaata gaaaatggct atttgtttgt gcagccgctc
      301 ttttttcttt cactgcctac acacagttga cgtttgcaga cagacaagaa agcacggata
      361 gcacagaaca aacaatccaa acgtcagata gcagcacgat tcaaagcagc gatgaaaccg
      421 tcgattcgtc aacagttgat tcttcagaag aacctaaaat tgctgaagac gatcaaatag
      481 tcaa
//
LOCUS       DACCKU010000258          479 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.273, whole genome shotgun sequence.
ACCESSION   DACCKU010000258 DACCKU010000000
VERSION     DACCKU010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.273"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>479
                     /locus_tag="IVN13_003043"
     CDS             <1..>479
                     /locus_tag="IVN13_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP7013446.1"
                     /translation="MRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDW
                     LFSMKKVTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNML
                     KREDEVFSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKND"
BASE COUNT          179 a           79 c           95 g          126 t
ORIGIN      
        1 gatgcgttct gcaggagatg ctatgaaaaa caagaaagtc gtctttatta tttctcctca
       61 gtggttcgtt aaaaatggtg taaaaactga ttactttaat acgtattatt ctgaacttca
      121 aacatacgac tggttgtttt caatgaagaa agtaacgcct gctgatcgct atttagctag
      181 aagattatta actttctcaa aagtaaaaga gaatgatacg ttgacggcga ttctgcaaac
      241 gatcaaaaaa gggaagctac ctttacctga atctttgaat cagctgcgca gccaatggaa
      301 catgctgaag agagaggatg aagtattcag caatattggg ttgaaagatc gacagcaaaa
      361 aatcgatcat gagtcaaaac gattaccaaa acaataccaa gaaacagaat tatccatttt
      421 agcaaatcaa attggagaac gagaaacgac gaataatcca ttcgggttga aaaacgatt
//
LOCUS       DACCKU010000259          476 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.274, whole genome shotgun sequence.
ACCESSION   DACCKU010000259 DACCKU010000000
VERSION     DACCKU010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.274"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>476
                     /locus_tag="IVN13_003044"
     CDS             <1..>476
                     /locus_tag="IVN13_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013447.1"
                     /translation="TSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGKKFHEE
                     RVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKEYVAWYEFSQRVLKDEEGNIVYRKK
                     RGETHLPELEKEAVLTLDEDKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHIIVS"
BASE COUNT          166 a           73 c           92 g          145 t
ORIGIN      
        1 acttctcaat cgaccattga ttacaagtcc attggttttc cgaagtattt ggatctcaaa
       61 ttacatgaaa gcctattaaa tcaacgcatt gtcgtggaat tttatcataa gggaaagaaa
      121 tttcacgagg aacgagtcaa agttggctat cgtggttttt gtgaaatcaa ctttgattgt
      181 catttagagc agggagatca tgtttgctta aaatacaatc atcaaggaaa agaatatgtt
      241 gcttggtatg agttttctca aagagtattg aaagacgaag agggaaatat cgtttatcga
      301 aaaaaacgag gagaaaccca tttgccagaa ttagaaaaag aagccgttct aacattagat
      361 gaggataaaa gtaacttatt agtagagtct attcggatgc actataccga taaaccaatg
      421 gctattttta tgctcacacc tgattatgat ccatctaacc atatcattgt tagtat
//
LOCUS       DACCKU010000260          460 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.275, whole genome shotgun sequence.
ACCESSION   DACCKU010000260 DACCKU010000000
VERSION     DACCKU010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.275"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..428)
                     /locus_tag="IVN13_003045"
     CDS             complement(<1..428)
                     /locus_tag="IVN13_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP7013448.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           91 c           59 g          178 t
ORIGIN      
        1 gctcttaaac gaataacttc ttcttcaaga gactcatctt taagtttttt aggaatgttt
       61 agcttggaat tcatactaat ttttctgccc cttttttggc tctcaagtga agaagcaccg
      121 ccttcttcat attgctctat ccatttactt agagttctat ttgaaccgat attaaatttt
      181 ttagcagttt cttggataga aagaccattt gtttccatat attctataac atcaagttta
      241 aattttgtag tgtagctttt gccacctcca accaagcctt cccaaccatg atagttataa
      301 atccttaccc aatgtctaac cagtgtacga tttatttgat atttatgtgc aagatatttg
      361 tagccgcctt cgttatttaa atagtctgaa actacttttt tcttaaaaac aaatgtatat
      421 ttccgcaaaa aaagcacccc ttttaattag atttctagtc
//
LOCUS       DACCKU010000261          456 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.276, whole genome shotgun sequence.
ACCESSION   DACCKU010000261 DACCKU010000000
VERSION     DACCKU010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.276"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            200..>456
                     /locus_tag="IVN13_003046"
     CDS             200..>456
                     /locus_tag="IVN13_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013449.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMG"
BASE COUNT          153 a           78 c           85 g          140 t
ORIGIN      
        1 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
       61 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
      121 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
      181 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
      241 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
      301 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
      361 tattgccgtc acaggtcact tcaataaacg aaatcagcta gttattgcta gcatgtatgt
      421 tagaaatccc gatcatttta cgcgatcgat gggcat
//
LOCUS       DACCKU010000262          450 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.277, whole genome shotgun sequence.
ACCESSION   DACCKU010000262 DACCKU010000000
VERSION     DACCKU010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.277"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..302)
                     /locus_tag="IVN13_003047"
     CDS             complement(<1..302)
                     /locus_tag="IVN13_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP7013450.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          140 a           79 c           78 g          153 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt cttcgagtta ccaacggtaa ccctcatacc gaacggcatt
      421 catcacccac ctatagagga tgggcgactt
//
LOCUS       DACCKU010000263          415 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.278, whole genome shotgun sequence.
ACCESSION   DACCKU010000263 DACCKU010000000
VERSION     DACCKU010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.278"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..390
                     /locus_tag="IVN13_003048"
     CDS             <1..390
                     /locus_tag="IVN13_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP7013451.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFD
                     TYNQKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          161 a           74 c           65 g          115 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
      301 tttgatacat acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc
      361 gatacattag tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatt
//
LOCUS       DACCKU010000264          415 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.279, whole genome shotgun sequence.
ACCESSION   DACCKU010000264 DACCKU010000000
VERSION     DACCKU010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.279"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..339)
                     /locus_tag="IVN13_003049"
     CDS             complement(<1..339)
                     /locus_tag="IVN13_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013452.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DACCKU010000265          405 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.280, whole genome shotgun sequence.
ACCESSION   DACCKU010000265 DACCKU010000000
VERSION     DACCKU010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.280"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
BASE COUNT          185 a           52 c           63 g          105 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaa
//
LOCUS       DACCKU010000266          405 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.281, whole genome shotgun sequence.
ACCESSION   DACCKU010000266 DACCKU010000000
VERSION     DACCKU010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.281"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..>405
                     /locus_tag="IVN13_003050"
     CDS             <1..>405
                     /locus_tag="IVN13_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HAP7013453.1"
                     /translation="KGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFN
                     SEFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKN
                     FFKSITFDCGKEFSNWKAISNQHDIDIYFADPG"
BASE COUNT          137 a           77 c           72 g          119 t
ORIGIN      
        1 taaaggaata tttgatattg acacactacc gatgaaaggt aaaagaaaac ccaatggcca
       61 tcaggaaaaa cggggaaaac aacagtatca gcgctcaatc catgatcgac ctgataatta
      121 tcctgatttc aattctgagt ttggtcacct tgaaggtgat acgatcgttg gcattcatca
      181 taaaagtgcc gtcattactt tagttgaaag attatctaaa gtcattatca cgattaaacc
      241 caacggccgt aaggcattag atattgaaac tgcccttaat caatggtttt ctcgcttccc
      301 taaaaacttc tttaaatcta ttacgtttga ctgtggaaaa gaattttcta actggaaagc
      361 cattagtaac caacatgata ttgatatata ttttgcggac cctgg
//
LOCUS       DACCKU010000267          393 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.282, whole genome shotgun sequence.
ACCESSION   DACCKU010000267 DACCKU010000000
VERSION     DACCKU010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.282"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            141..>393
                     /locus_tag="IVN13_003051"
     CDS             141..>393
                     /locus_tag="IVN13_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013454.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLL"
BASE COUNT          177 a           49 c           56 g          111 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa gcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tac
//
LOCUS       DACCKU010000268          361 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.denovo.285, whole genome shotgun sequence.
ACCESSION   DACCKU010000268 DACCKU010000000
VERSION     DACCKU010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.denovo.285"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..>361)
                     /locus_tag="IVN13_003052"
     CDS             complement(<1..>361)
                     /locus_tag="IVN13_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013455.1"
                     /translation="DKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRISQKIFYSGLAY
                     LPLNYVVTRIPEAFHRFEIIPGSLQGVNIFQLQEMPQLFHFDSTGFWNPLTPTTSLGT
                     RLVVSTICVVPAVLMGL"
BASE COUNT          125 a           62 c           62 g          112 t
ORIGIN      
        1 ttcaatccca taagtacagc tggaacaaca caaatcgtag aaacaactaa acgtgtgcct
       61 aaacttgttg ttggggtcaa aggattccaa aaaccagttg aatcaaaatg gaataactgc
      121 ggcatttctt gtaactgaaa aatattcact ccttgtaaac ttcctggaat aatctcaaat
      181 cgatgaaatg cttctggtat tcttgtaaca acatagttta aaggaagata agctaagcca
      241 ctataaaaaa tcttttgaga aatacgtttg tcatgttttt ctggtagctc ttctcctgaa
      301 ggagtatagc gatagaaatg agaaagatac ctattctttt ctaaattgaa tcgtttgtct
      361 t
//
LOCUS       DACCKU010000269        29817 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.027, whole genome shotgun sequence.
ACCESSION   DACCKU010000269 DACCKU010000000
VERSION     DACCKU010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.027"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..431
                     /locus_tag="IVN13_003053"
     CDS             <1..431
                     /locus_tag="IVN13_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7013456.1"
                     /translation="DFELPANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVET
                     LIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPE
                     KELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQVKAILI"
     gene            459..914
                     /locus_tag="IVN13_003054"
     CDS             459..914
                     /locus_tag="IVN13_003054"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7013457.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            911..1669
                     /locus_tag="IVN13_003055"
     CDS             911..1669
                     /locus_tag="IVN13_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7013458.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            2170..3369
                     /locus_tag="IVN13_003056"
     CDS             2170..3369
                     /locus_tag="IVN13_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013459.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            3391..3603
                     /locus_tag="IVN13_003057"
     CDS             3391..3603
                     /locus_tag="IVN13_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7013460.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3678..4154
                     /locus_tag="IVN13_003058"
     CDS             3678..4154
                     /locus_tag="IVN13_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7013461.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4151..5959
                     /locus_tag="IVN13_003059"
     CDS             4151..5959
                     /locus_tag="IVN13_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7013462.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            6036..6251
                     /locus_tag="IVN13_003060"
     CDS             6036..6251
                     /locus_tag="IVN13_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7013463.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            6343..7206
                     /locus_tag="IVN13_003061"
     CDS             6343..7206
                     /locus_tag="IVN13_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013464.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            7237..7779
                     /locus_tag="IVN13_003062"
     CDS             7237..7779
                     /locus_tag="IVN13_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7013465.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            7793..8215
                     /locus_tag="IVN13_003063"
     CDS             7793..8215
                     /locus_tag="IVN13_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7013466.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            8145..10544
                     /locus_tag="IVN13_003064"
     CDS             8145..10544
                     /locus_tag="IVN13_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7013467.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10576..12567
                     /locus_tag="IVN13_003065"
     CDS             10576..12567
                     /locus_tag="IVN13_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7013468.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12590..12841
                     /locus_tag="IVN13_003066"
     CDS             12590..12841
                     /locus_tag="IVN13_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7013469.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12831..14060
                     /locus_tag="IVN13_003067"
     CDS             12831..14060
                     /locus_tag="IVN13_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7013470.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            14057..16138
                     /gene="topB"
                     /locus_tag="IVN13_003068"
     CDS             14057..16138
                     /gene="topB"
                     /locus_tag="IVN13_003068"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7013471.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            16287..20207
                     /locus_tag="IVN13_003069"
     CDS             16287..20207
                     /locus_tag="IVN13_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7013472.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            20208..21152
                     /locus_tag="IVN13_003070"
     CDS             20208..21152
                     /locus_tag="IVN13_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013473.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            21156..21359
                     /locus_tag="IVN13_003071"
     CDS             21156..21359
                     /locus_tag="IVN13_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013474.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21659..21895)
                     /locus_tag="IVN13_003072"
     CDS             complement(21659..21895)
                     /locus_tag="IVN13_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013475.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(22078..22449)
                     /locus_tag="IVN13_003073"
     CDS             complement(22078..22449)
                     /locus_tag="IVN13_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013476.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(22446..23774)
                     /locus_tag="IVN13_003074"
     CDS             complement(22446..23774)
                     /locus_tag="IVN13_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7013477.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23735..24064)
                     /locus_tag="IVN13_003075"
     CDS             complement(23735..24064)
                     /locus_tag="IVN13_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7013478.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(24322..24693)
                     /locus_tag="IVN13_003076"
     CDS             complement(24322..24693)
                     /locus_tag="IVN13_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7013479.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            24838..25023
                     /locus_tag="IVN13_003077"
     CDS             24838..25023
                     /locus_tag="IVN13_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7013480.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25535..25753
                     /locus_tag="IVN13_003078"
     CDS             25535..25753
                     /locus_tag="IVN13_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013481.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            25966..26625
                     /locus_tag="IVN13_003079"
     CDS             25966..26625
                     /locus_tag="IVN13_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7013482.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26628..27971
                     /gene="vanS"
                     /locus_tag="IVN13_003080"
     CDS             26628..27971
                     /gene="vanS"
                     /locus_tag="IVN13_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7013483.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            28142..28949
                     /gene="vanY"
                     /locus_tag="IVN13_003081"
                     /pseudo
     CDS             28142..28949
                     /gene="vanY"
                     /locus_tag="IVN13_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
     gene            28967..29803
                     /gene="vanW"
                     /locus_tag="IVN13_003082"
                     /pseudo
     CDS             28967..29803
                     /gene="vanW"
                     /locus_tag="IVN13_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         7675 a         7670 c         7997 g         6475 t
ORIGIN      
        1 tagattttga attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga
       61 tggagcctgg gaaaatacag gaaatcccct acacggtctt taaccgaaca gataaatcag
      121 taacagttga gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg
      181 gagaatcaaa aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa
      241 cagtgggaga aataactgtt cctcctcaaa gtgcggtcac tgagacactt tcactgaaag
      301 cgccagaaaa agagctggct ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag
      361 agaacgtttc taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa
      421 tcttgatttg aaatagcgaa aaagagtctt ccccagattt gaaattaaaa aaagcttatg
      481 cggatcaaag cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt
      541 atatgttcgg cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc
      601 tttcaataaa aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc
      661 cattagaagg aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga
      721 aagaaaaacg ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat
      781 tgaatgaaaa ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga
      841 tacttgttgg agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa
      901 ggaaaaagaa atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt
      961 atgtacacaa agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc
     1021 tgtgaccaaa ccagatgctg caaatcctga agacgttgtt ccagttcctc ctgttgatcc
     1081 aaatgatcct gatactcctt ttacacctga cgaaccaaat cagggaacat ccggattact
     1141 gacgattgac caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac
     1201 agtatatgct gcattacaaa gcggaacgga cagtactgga gcacaaacac aagtacagaa
     1261 tttcgtacaa gtaacggata aaagcggaaa ttatgccggt tggaaattat cagtcaaacg
     1321 aacagaattt attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt
     1381 caaaaacgcc gtgattcgaa atagcgcaac taatagcagt ggaatgccat ctaccgtttc
     1441 tggaactggc gctgaaggaa tcgaaatccc agcagatcag gcagtagatc tagtaactgc
     1501 aggagcaaat gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg
     1561 gagacaatca gtggaattat ttgttcctgt atccaattat gctgtagaca actacgtttc
     1621 aacattcaca tggtcgttaa cagatgctcc tacagaagag gaagcgtaaa atcatatcaa
     1681 cgatttcagg gagtggagcc gattcaaggc ccactccctg aaatctgcta tgcttacgtc
     1741 tcagctctta ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc
     1801 ttttcaaaga tctttcgacc aacgagtaag gagtcaccgt tgaaaaattc taacacataa
     1861 tgagcagaat caattttctg caattgcgat aagttgatac agtaagattt taaaagtaaa
     1921 aattttaggt tatcgctgaa agccccggaa atacgggcat ttcgcaggat atgagaactc
     1981 aatttactac caatttacta ctttcggatt gagccgcata tatgctgaac catactgcta
     2041 atacccgcca ccccaaatac ggcacatcat aaattgaata cgacaccgta aacgcggcgt
     2101 tcctctttcc cgtcatggga gaacaggacg ccgctttttt atgcccaaat agagaaagga
     2161 ggcgacgcca tgctatttac ttccggccct gaccgggcgt ttgaacggct gatgcaggaa
     2221 aagcccggtt tcgatcacta cgacggcggc ggggcggacg cgccggagga ttgcggcacc
     2281 tgccgatttt accgcccgga ttggaaatat caattctgcc agtttgcaga gtgtccctat
     2341 cagccgggca agctgaccga ctatgcagcg gtcaaattca cagtgaaagg agcgaacaac
     2401 gctatggcag ttttccgtgt ggaaaagaac aaaggctata cggtgatgtc aaatcaccac
     2461 ctgcggaata agaacctgac cttaaaggcc aagggcctgc tgtcgcagat gttgtccttg
     2521 ccggaggatt gggactatac cctaaaagga ctgtcctata tcaaccggga aagcatcgac
     2581 gcgatcagga cagcggtatg ggagcttgaa aaagctggat atatccggcg tgagcagggc
     2641 cgtgacgcaa aaggcaaaat ggccgatatg gtctatacca tctacgaaca gccggtattg
     2701 gaaaacccgg tattggaaaa tccaacatcg gataacccgg tgttggaaaa tccgacatcg
     2761 gataatccgg cgtcggaaaa tccaacgcaa ttaaataaag aagtacaaag aactgactta
     2821 tcaaaaaaag aaaaaataat tacagatgga caaagtaccc attccattcc tatcccatcc
     2881 ccgacccctg cccctatgga gggcgaggca gcggctacgc cgccggaacg gaaaaggaca
     2941 ggaaaggatg aggctgtacg gatttatcgg gaaatcattt tggagaacat cgagtatgcc
     3001 tatctcattc aggacaggag cattgaccgt gaacagcttg acgaaatcgt agacctgatg
     3061 ctggaaaccg tctgcaccgc ccgaaagaca atccgtgtcg ccggggacga ctaccctgcc
     3121 gagctggtga aatccaagta catgaagctg accggcgaac atatccgctt tgtccttgac
     3181 tgtctgcggg agaacacaac gaaagtccgc aatatcaaac aatacctgcg ggctatgctg
     3241 ttcaacgccc ccagcaccat ttccaactac tacacggccc ttgtcgcgca cgacatggca
     3301 cagcccgatt ggggaaagcc taaatccggc ctgccggact attcctgcgg cgagggcgaa
     3361 agcctgtaac gagaaaagga ggacgaccct atgaagcagg gcgcattgat ttttgacgag
     3421 cgaacagacc gttacgacat ccgctttgat ctggccgact attacggcgg cctgcattgc
     3481 ggggagtgtt tcgacgtgat gatcggcggc aaatggaggc ccacccgcat tgaaatggcc
     3541 gaaaactggt atcttgtcgg catccgggcc gacgaccttt ccggcctgcg ggtgcggatt
     3601 tgacctatat ctacctgcgg cgtctggtgg ttccgcctta gacgccgctt ttgactttct
     3661 gacaaggagg gatgcgattg caggaagaag ttactcaaaa gaccattgcc ctgtccatga
     3721 aaacgggcaa gctcacggct caagtgctgc aagcggcgct gaaaaagtac ctgcaacacc
     3781 gggccaaggg caaaaacacg ctgcaccacg gccagcagag cctaaagcag cttaaaaagc
     3841 atggggctga tctctccaac attgagatca ccgaggccaa cattggagcg ttcaagccat
     3901 gcgccaagaa atacggcgtg gattttacct tacgaaaaga taagaccacc cagccgcccc
     3961 actacgttgt gattttcaag gccaaggatg cagataacct tgatctggcg tttcgggagt
     4021 ttaccgccaa gacgctttcc aaggagcagc ggccctctat ccgcaaggtt ttgtccgctg
     4081 tgaagcaaaa ggcggcggcc cagaccaagc agcgggccaa ggagaaaatc aaggaaaggg
     4141 ggctggaact gtgaagcctg acgtaaagaa attgctgatc ctgaacctcc cgtatctgat
     4201 ttttgtgtac ctgtttgcga aatgcgggga ggcgtaccgg ctggccgctg gtgctgacct
     4261 gtcggggaag ctcctgcatt ttgcggatgg ttttgcggcg gcttttgcca acccgctgcc
     4321 aagcctgcac ctgtttgacc tgtgtatcgg tatcgccggt gccgtgatcg tgcggctggt
     4381 ggtgtactgc aagagcaaga acgtgaagaa ataccgcaaa ggcatggagt atggcagcgc
     4441 ccgctggggg acggccaaag acatagcgcc ttacatcgac cccaagtttg aaaataatat
     4501 cctgctcacc caaacggaac ggcttaccat gaccggcagg cccaaagacc ccaagacagc
     4561 gcggaataaa aacgtgctgg tgatcggtgg cagcggttca gggaaaacga gatttttcgt
     4621 gaaacccaac ctgatgcaat gctttccgac cacggactat ccgacttcgt ttgtggtgac
     4681 agatccgaaa ggcactttgg ttctggaaac cggcaggatg ttccagcagg cgggctaccg
     4741 cattaaaatc ctgaatacca ttaacttttc caagtccatg aagtacaacc cctttgtcta
     4801 catccattcg gaaaaggacg tgctaaagct ggtaaatacc ctgatttcca acaccaaggg
     4861 cgagggggaa aagtcagcgg aggatttttg ggtgaagtcg gaacggttgt tttattcggc
     4921 cctgatcggc tatatctggt acgaggcccc agcggaggaa atgaacttct caacgctgct
     4981 ggaaatgata aacgccagtg aagcccgcga ggacgacccg gagtttcaaa gccccgtcga
     5041 tctcatgttt gagcgtttgg agcagaaaga ccccgaccat tttgcggtgc gccagtacaa
     5101 gaaattcctg ctgtccgcag gcaagacccg ttcttccatc ctgatttcat gcggtgccag
     5161 acttgcgccc tttgacatcc gggaggtacg cgagctgatg gaggacgacg aaatggagct
     5221 ggacaccatc ggggacgaaa agacggtgct gtatctcatt atgagcgata cggacacgac
     5281 ctttaatttc attctggcta tgctccaaag ccagctaatc aacctgcttt gtgaccgggc
     5341 tgatgatgtg tacggcgggc ggctgcctgt ccatgtacgc ctgattttgg atgaatttgc
     5401 caacattggc caaataccca atttcgataa actcattgcc accattcgaa gccgtgaaat
     5461 atcggcctct attattttgc agagccagtc gcagttaaaa gcgatttaca aggacgccgc
     5521 cgagatcatt tcggataact gcgatagtgt gcttttcctt agtgggcgcg gcaaaaatgc
     5581 caaggagatt tccgatgcgc tggggaaaga aacgattgac agtttcaaca ccagcgagaa
     5641 ccggggaagc caaacgtccc acggattgaa ctatcaaaaa ttaggaaagg agttgatgtc
     5701 ccaagacgaa attgccgtga tggacggcgg caagtgtatc ttgcagctac ggggtgtgcg
     5761 cccgttttta tcggagaaat tcgatattac caagcatccc cggtacaaat accttgccga
     5821 cgccgacaag aagaatacct ttgacgtgga cagatttttg actatcacgc gccggaaacg
     5881 gcagcaagtg gtaacgcagg atgaagtatt cgacctgtat gaaatcgacc tgtcggacga
     5941 ggacgccgcc gctgaataaa cagcggcttt tttcattcca acaacaaaat caaaaaatca
     6001 ttatgagccg gaaaccggca gaaagaggta aatttatggc attttttaat tccgcagtta
     6061 ctactttgca gactatcgtg atcggactgg gcggcgctct gtgcgtatgg ggcggcatca
     6121 atctgttgga gggatacggg caggacaatc cgggggcaaa atcgcagggt gtcaagcagc
     6181 ttgtcgctgg cggcggcgtg gccctgatcg gtgtcactct ggttcccctg ctgtccgggc
     6241 tgctgggctg atcctggcct ttgtccctgt ccaaaccctt aactaatcgc ctccgcgtcc
     6301 tcccggccag accgggaggg cgcgggaaag gaggtgctac ccgtgattag tgaaatcatt
     6361 gaagaatgga taaaaggcat cctcatggac ggtatcacgg gcaacctgtc cgggctgttc
     6421 gatacggtaa atgccaaagt cggagaaatc gccgccgacg tgggacgaac cccgcaggca
     6481 tggaacagcg gtattttcaa tatgctgcac agcctgtcgg aaaccgtgat cgtccccata
     6541 gccggggcca tactcgccct tgtcatgtgc tatgagctta tccagatgat aacggaaaag
     6601 aacaatatgc acgactttga cagctccatg ttcttccgct ggatattcaa atccgcgttt
     6661 gcaatcctga tcgtaacgaa cacatggaac atcgtcatgg gcgtattcga cgccacccaa
     6721 agcgtcgtca atcaaagcgc cggtgtgatt atcgggcaga catccattga ttttaacacc
     6781 ctgataccag accttgaaag ccaattggag gctatgagcg tgggcgctct tttggggctg
     6841 tggttccaaa cccttgttgt gggcctcacc atgaatatcc tttccatctg cattttcctt
     6901 gtgacctatg gccgcatggt ggaaatctat gttgtcacgt cgctggggcc tatccccctt
     6961 gccacaatgg gcaacgcgga atggcgcggc atgggccaag ggtatctgaa atccctgctg
     7021 gcgctgggct tccaagcctt tttaatcatg gttgtggtgg gtatctacgc ggtgctgata
     7081 cagaatatcg gttccgcttc cgacatatcc ggcgcaatct ggggctgtat gggctatacg
     7141 gtgctgctgt gcttctgcct gttcaagacc ggcagtatat caaaggccgt attcggggcg
     7201 cattgatccc aaaatccaag catacacagg aggaacttgt ttatttcaac gtacaacatc
     7261 atttacgctg acccgccgtg gcggtatgac tgcaaaaacg tacagggcgc agcggaaaat
     7321 cactattcca ccatgagcat tgacgagctg tgcgccctgc cggtggaacg gctggcgtcc
     7381 aaagactgcc tgctgttttt gtgggccaca ttccccatgc tgccggaagc tctgcggtta
     7441 atcaaagcgt ggggcttttc attcaagacc gtggcttttg tatggctgaa acagaaccgt
     7501 aaaagcctta catggtttta cgggctgggc cgctggacaa ggggaaacgc ggaaatctgc
     7561 ctgctggcaa cacacggcaa gccgaagcgt cgttctgcct ctgtccatca gtttattatc
     7621 agcccgattg aacaacacag caaaaagccg gatgtgaccc gtgaaaaaat cgtagagctg
     7681 gcgggcgatt tgccccgcgt ggagctgttt gccagacaga aaacccccgg ctgggacgtg
     7741 tgggggaacg aggtggaatg tgaccttatc ctgacgtgaa aggaggtgcc aaatggctta
     7801 tgtaaccgtc cccaaggact taacccatgt gaaaagcaag gttgtgtttg ggcttaccaa
     7861 acggcaactg atctgttttt ccggggcgct ggtgatcggc gtccccttat attttctcac
     7921 ccgtgactat cttaccaaca gcgcggcggc cctgctcatg gtatttgcca tgctgccggg
     7981 ctttttgctt gccatgtacg agcgtcacgg ccagcccctt gaagtggtga ttatgcagat
     8041 catccagtgc tgcttcatcc agccaaagga gcgtccctat cagaccgaca acacctacac
     8101 cgcccttgtg cggcaatacc agatggaaca ggaggtaaag gccattgtcc aaaaaggaaa
     8161 tcccaagaga aaccgaaaaa acaaagctga cccgcgccca gcgaaaagaa attgacgcgg
     8221 taatccgtaa atataagggc gacggcaggc cccacacggc gcagcagagc atcccctacg
     8281 aggtcatgta cccggacggc gtgtgccggg tatcgcccgg cgtcttttcc aagtgcattg
     8341 agtttgcgga catcagctat cagcttgccc agccggacac gcagacggct atctttgaaa
     8401 aactgtgcga cctgtataac tatgtggacg cctccattca tatccagttt tctttcctca
     8461 atcggaaagt tgacccggtg cagtacgcca agagctttga gatcgcgccg cagggggacg
     8521 acttcgacga catccgggcc gagtacaccg gcattctgca aaagcagctt gcaaacggca
     8581 acaacggcat ggtaaagacc aaatacctga cattcaccat cgaggccgaa agcgtgaaag
     8641 cggcgcgagc cagattgaag cgcatcggct ttgacctgtt aggctatttc aagagcatgg
     8701 gggccgtagc ccatgtgatg gacggctggg aacggctgaa cctgctgcat ggcgtgtacc
     8761 acccggacgg cgagatattc aattttgact ggaagtggct ggccccgtcc ggcctctcca
     8821 ccaaggattt tattgccccg tcctccctgt gcttcggcaa tgcaaagaca ttcggcatgg
     8881 gcggcaaata cggcgcggta agtttcctgc aaatcctgtc cccggagctg tcggacgata
     8941 tgctggccga cttcctcaat acggaaagcg gtgttttagt caatctccat gtgcaggcca
     9001 ttgaacagac caaggcgatc aagaccatca agcgcaaaat taccgacctt gacgctatga
     9061 agattgcgga gcagaaaaag gccgtccgca gcggttacga tatggacatt cttcccagcg
     9121 accttgccac ctacggcgag gacgccaaga agctgctgac aaagttgcag acccgcaatg
     9181 aacggctgtt ccagttgacg ttccttgtcc tcaacgtggc cgacaccaag caaaagctga
     9241 ataacgacgt gttccaagcc gcaggcgtgg cccaaaagca taactgtccc cttgtccggc
     9301 tggactacca gcaggaacag ggccttgcaa gcagcctgcc gctgggcgtc aatcagatca
     9361 agatacagcg cagccttacg acttccagcg tggccgtgtt cgtccccttt gtgacgcagg
     9421 agctttttca gggcggcgcg gccatgtatt acggcatcaa cgccaagtcc cgcaacatga
     9481 ttatgctgga caggaagcag gcccgttgcc ccaatgcctt gaagctcggc acccccggca
     9541 gcggcaaatc catgagctgt aaatccgaga tcgtcagtgt gttcttaacg acgcctgacg
     9601 atatttttat ttcagacccg gaggccgaat attaccccct tgtcaaacgg ctccacggac
     9661 aggtaatccg gctgtcgcct accagcaagg actttgtgaa cccgctggac atcaacctga
     9721 attattcgga ggacgataac ccgctggccc tgaaatccga ctttgtgctg tcgttctgtg
     9781 agcttgtcat gggcggcaag aacgggctgg aagccattga aaagaccgtg atagaccggg
     9841 ctgtgcgcgt gatctaccgg ccctatctgg cagacccccg cccggagaat atgccgatcc
     9901 tgtccgacct ccacaaagcg ttgttagatc agcatgtccc ggaggctgac cgggtggcgc
     9961 aggcccttga cctgtatgtg tccggctccc tgaacgtatt taaccaccgt accaacgtgg
    10021 acatcggcaa ccggcttgtg tcctttgaca ttaaggagct gggcaagcag ctcaaaaagc
    10081 tggggatgct cattgtccaa gaccagatat gggggcgcgt cacggccaac cgaagccaag
    10141 gaaaggccac atggtatttt gccgacgagt tccatttgct tttgaaagag gaacagacgg
    10201 cggcctattc tgcggagatt tggaagcggt ttaggaaatg gggcggcatt ccaacaggtg
    10261 ccacccagaa cgtcaaagac ctgctttcct ccccggaaat tgaaaatatc cttgaaaaca
    10321 gcgacttcat tacgctgctc aatcaggcgt ccggggatag aaaaatcctt gcggaacgct
    10381 tgaatttgtc caccgaacag cagaagtaca tcgacaattc cgaacccggc gagggccttt
    10441 tgatctttga aaacgtggtg ctgcccttta ccaaccccat cccgcacaac acccagcttt
    10501 acaagattat gacgacccgc ttaaacgagg tggcgggagt atgacccggc gacgccacaa
    10561 aggaggtgaa ccaccatgaa agagctgaaa gccaaggcga aagtgacgca ggccatgacc
    10621 cgcgacgggc tgacgctgga aaatcaggcc accggcgagg tggagaacat ttccagccgg
    10681 gaggccgaac aggatttgtc cgcgccgggg ccgggcggca ccgccgataa gctgctggaa
    10741 cgggccgacg ccatccatga cggccataag gccaagaaag ccgcgaagca ggccggggct
    10801 gtggccgccg agggggacgc tgccctgcac cggccatccg cccggctcca actgtcggag
    10861 gaagaacggg ccgacccgga tttacagccc tatatccgaa aatcggacaa acgggccgat
    10921 aagctggata aggcccgcgc cgccatcccc aagaagcgcg tcccggtcaa ggaaaccgtc
    10981 tttgacgagg caagcggcaa agcaaagacc gtcctgcact ttgacaaaca ggacaagggg
    11041 cctccctccc tgaaacccaa tccggcaggc cgtcctgttt ccgaggtttt tctattcggc
    11101 cacgggaaaa tccatgaagt cgagcatgaa aacgtgggtg tggagggcgg ccataagggg
    11161 gaggaacttg ccgagcggca ggccggaaaa gccatccgca gcaccatccg gcaccacagg
    11221 ctgaaaccct actgggacgc ggaaaaggcc gagcgcaggc tattgtctgc caacgcggag
    11281 tatttctacc agaaatcttt gcgggataac ccggagatcg cacaggcggc cagcaacccc
    11341 atttcccggt tctggcagaa acagcagatc aagcgccagt acgccaaggc cgcccggaca
    11401 gcgggccaga ccgcaggcgc ggcggcacag ggcgcggcca agacagccgg ggcctccacg
    11461 aaagcggcca aggccgcagc caaagaaagt gaacgggcgg cctcctttgt ggcccgccac
    11521 tggaaagggg cgctggccgt ccttgtgttg gggcttttga tgctgcttat catcggcggc
    11581 ttacagtcct gcaccgccat gttcggcagc gccggaagcg gcatcacggc gtcctcttac
    11641 ttttccgagg atagcgatat gttggccgcc gaggacgcct acgcggagct ggaagccgag
    11701 ctgcaagact atctggacaa ctacgaggcc acccatgact atgacgaata ccattttgac
    11761 cttgacgaga tcagccatga cccctatgtg ctgatttcca ttctgtcggc gctccatgac
    11821 gggatattta ccatcgggga ggtacagggt gatttgtcca tgctctttga caagcaatat
    11881 atcctgacag aaaacgtcgt ggtagaaacc cgttaccgca ccgaaaccga tacatggacg
    11941 gacgcggagg gcaacaccca tacagacacc taccaagtgc cgtatgacta ttacatctgc
    12001 tacgtcacgc tggaaaactt cgacctgtcc catgtgccag tctatatcat ggacgaggaa
    12061 acgctaagtt tctattccct gtatatgtcc acgctaggaa accggcctga tctgttttcc
    12121 ggcagccagt accccaacgc ctccaccctg acgccgccga cctactacga cgtacccccg
    12181 gaggctttgg aggatgccac ctttgccgcc atgctcaaag aggcggaaaa atatatcggt
    12241 tatccctatg tgtggggcgg cagctctccc agcaccagct ttgattgcag cggcttcatt
    12301 tcgtgggtaa tcaatcatag tggatggaac gtgggacggc tgggggcgca gggcctatat
    12361 aacatctgca cccctgtttc cagttcgcag gccaagcccg gcgaccttgt atttttcaag
    12421 ggaacctacg acacccccgg cgtgtcccac gtcggcctgt atgtgggcaa caatgtgatg
    12481 ctgcattgcg ggaacccgat ttcctacacg aaccttactt caaactactg gcaatcccat
    12541 ttttacagtt acgggcgttt accttaacgc cagaaaggag ctttcaatta tggcaaccaa
    12601 acttgaccgc atcgaaaagg acatccagaa aaccaagtct aagatcgcgg agtttcagaa
    12661 acagcttcgg gagctggaaa cgcagaaaac cgaacaggag aatttgcaga tcatccagct
    12721 tgtgcgcggc atgaacatga agccggagga atttgccgcg ttcttacgca gcggcgcaat
    12781 gcaggccgcc cctgccgcaa ccccttacca tgaacaggag gacaatgccc atgaagaata
    12841 agaccagacg gatttcccgt attctccccg cacttctggc cgtgatgctc tgcgtcacgg
    12901 cctttcccgt taccgccctt gcaggcggca atgacccggc accggccccg ctgccggagg
    12961 ctacaaccga acccgctacg ggcggtttgg agcctgaaac agacgaaacc ggcaccgcca
    13021 cggacgggac agaaaccgag gccgaaaaag ataccggccc tctgaccggc aaggacatca
    13081 ccgacctgtt ttccgcgctg ttcggttcca aggtgagcat tgccgccacg gacgacggca
    13141 tccagatcac caccggcacc gacaaaaaag aacctgaaca gaccggcacc gtcaccacca
    13201 acggcggcag gctgaatgtc cgcaccggcg cggggcttga caagaccgcg ttcacccagc
    13261 ttcccaatgg gacgacggtt gaggtagtcg gcacagacgg cgactggatt aaaattctct
    13321 tgccggagcg catcggctat gtccattccg actatatgac cgtcagcgaa aaggaagtag
    13381 cggctaccgg cgagggcggt ttttccctct ccattgaccc ggaggaaatt gcttctctgc
    13441 tggaactgtt caatggcggc ggggccagcg ccgccctgtc cccggacggg aacctgtcac
    13501 tgattgacga catcggcagc gccgcgaaaa atggcaagca gtttatcacc gtggaaacca
    13561 aaaacggcaa tgtgttttat ctgattattg accgggacga cgagggaaaa gaaactgtcc
    13621 atttcttaaa tcaggtggat gaagccgatc tgctgacgct gatggaggac ggggaaaccg
    13681 tcacccccgc tgctgtctgc tcctgtacca cgaaatgcaa ggccggggct gtgaacacga
    13741 attgcccggt atgtaaaacc aacctgaccg agtgcagcgg cccggagccg caggaacccc
    13801 agccggagga acccgaagcc ccggaggaag aacccaaggg cggcgcgggc ggcctgatcg
    13861 tgttcctgct ggtggcgctg gcgggcggcg gtgcggccct ttactatttc aagttcaaaa
    13921 agcccaaggc tgaaaccacc ggcagcgacg atttgggcga gtatgatttt ggggaggacg
    13981 aggatttgga ggacgaggaa cccgaaaccg agtttgaccc ggaagctgat tttgtgagtg
    14041 aacaggagga cgaggaatga catataaact tgtaatcgcg gaaaaaccca gcgtggcccg
    14101 cagcattgcg ggcgtgatcg gggccacgga gaaacacgac ggctatttgg aggggaacgg
    14161 ctatctggtg agctggtgca tcggacatct ggtgtccttt gccgacgccg gacgctatga
    14221 cgagcgtttc aaaaaatggc gctatgagga tttgcccatt ctcccggaaa catggcagta
    14281 catcgtcccc gacgacaaga aacagcagtt tgacgttctc tgttcgctga tgcagcgccc
    14341 cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc gagggggaat tgattttccg
    14401 tttcgtctac cagatggcgg gatgccaaaa gccctttgag cgcctttgga tttcctctat
    14461 ggaggacagt gctattaagg acggctttgc ccacctgaaa ccgggcggcg actatgatcc
    14521 gctgtatcaa tcggccctgt gccgggccaa ggccgactgg ctgatcggca ttaacgccac
    14581 ccggcttttt tccgtcctgt accacaagaa cctgactgtg ggccgcgtcc agacgccgac
    14641 cttaaaaatg ctggtggaca gggacgccaa gattaccgat ttcaagaaag agaaatatca
    14701 catcgtccat atcacggcgg gcggcgcgga cgcggtaagc agccgctttt ccgacgctgc
    14761 cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag gccgtttgcg tttccgtgac
    14821 gcgggagaaa aagacggaac agccgcccaa gctctatgac cttaccacct tgcagcggga
    14881 ggctaaccgg cttttcggct ataccgccaa gcagaccctt gactatgccc aggcactgta
    14941 tctaaagaag ctgctgacct atccgagaac ggatagtcaa tatcttaccg acgatatgct
    15001 gcccgccgtg gaaagccttg tgtccggcct gtgggagctg gtgccgtttg ccaagggcct
    15061 gaacctttcc ccgcagtttg accgcatcct gaacagcaaa aaggtgtccg accaccacgc
    15121 cattatcccc accgctgaat ttgtgaagca gggctttggc actctggccg agagtgaaaa
    15181 aaagctgctg tccctgatct gctgcaagct cttatgtgcc gtggccgggc cgcatatcta
    15241 tgaggccgtc accgccacat ttacctgtgc cgggcaggag tttaccgcca agggcaagac
    15301 cgtcctttcc cccggctgga aagagattga acgccggttc cgttcttcct taaagaccga
    15361 cgccgacgag gacgccgagg cggtgcggga gctgccggag ctttgcgagg gccagacctt
    15421 tgcagacgtg gccgcctctg tcaccgagca tttcaccacg ccgcccaagc cctacaccga
    15481 agatacgctt ttgtccgcta tggaacgggc cggggccgag gatatgccgg aggatgccga
    15541 gcgcaaggga ttaggcacac cggccacccg cgccgccatt ctggaaaagc tggtgcagat
    15601 gggctttgtc cagagaaaag gcaagcagct cattcccacc aaggacggca tcaatctggc
    15661 tgtggtgctg ccggaggcgc ttacttctcc ccagcttact gccgaatggg aaaaccggct
    15721 gaccgagatc gccaaggggc aggccgatcc tgacgaattt atggacggca tcgagacgca
    15781 ggcgcgggag ctggtgcaga cctattcctg tatcagtgag gaaaagcaga aattgtttca
    15841 ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt gaggctgtct atgagggcaa
    15901 gaaaaactac tactgcggaa accgggcctg ccagttcgtc atgtggaaga atgaccgatt
    15961 ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc gctgcgctgc tcaaaaccgg
    16021 caaagtcaag gtaaagggcc tctattccgt aaagaccggc aaaacctacg acgggacggt
    16081 gcttttggct gataccgggg gcaagtacgt caactaccgc attgaacaca ggagctaaag
    16141 gacagtaaca cagcaagcat agggagtgtg gctacactcc cggtaaaatc ccccggccgc
    16201 gtccctgccg ggccggggat ttttgcttgt tggggaagtc cccaaccccc tattttttga
    16261 aacagaacag agaaaggagt gaccgcgtga acgaagtatt ttctatccaa gtccataacc
    16321 ggcagttttt ggaacagggc aaacaaggcg tctggctgga tttgcccacc acaaccgaaa
    16381 cgctgcaagc cgccctgcgg gatgttggca ttactgccga caatccgcag gattttttta
    16441 tcaacgggta ttcctgcccg gaggacagac accttgccat tccctatgac atggtgctgg
    16501 ccgccgacgt ggacgagctg aatttccttg ccgcccggct gggccagctt gacgccgccg
    16561 agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg cggctttgag aatatcggcc
    16621 agatcatcga ctacaccgaa aatgtggatt actatgtgca tcttcccgac gttagggggg
    16681 atggacagtt gggcgactac tacctgaacc gttccggcat ggtggatatg ccggaggaat
    16741 ggaaagccgg gattgatacg gcgcagtttg gacgccacat tgcccagcag gaacaaggcg
    16801 tttttaccca gtacggctat ctggtgagaa gcggcgacga atggcagcgc gtccatgagg
    16861 gccagccggt gccggaggaa taccgggtga tgcactaccc ccagccggaa gctgaccggg
    16921 aggaaccgca ggcccagcag ccggaggcat ccgaaagccc cgacaaagcg ccgcccccgg
    16981 ttatcccgat catgctggac ggcatgaaca gcaaagaacg catgaaagtg attactgacc
    17041 gtctggaaac cggcgtacag gagctttttg aaagtagccg ctatatggct tacttatccg
    17101 ctatgtccaa gatgcacgat tacagtctga acaatacgct gctgatcgcc atgcagggcg
    17161 gttcgattgt ggcgggctat aacaggtggc aggatgaatt tgaccgccat gtgaagcggg
    17221 gcgagaaagg cattaagata ctggccccgg ccccgttcaa ggttaagcag cagatggaga
    17281 aaatcgaccc ggccacccag cagcccgtga tcggcgcgga cggcaagccc gtgaccgaag
    17341 aacaggaagt gacggttccg gcctttaagg tggtgacagt ctttgacatc agccagaccg
    17401 agggcaagga ttttcccatg ccctatatca gtgaacttac cggcgacgtg cagcagtatc
    17461 aggatttctt tgccgcgctg gaacggacat caccctatcc cataagcatt gagccgattt
    17521 cagacggggc caagggccgc tgcttctacg caaaacagcg cattgccatc aacgagggca
    17581 tgagcgaatt gcagacccta aagaccgcca tccatgagat tgcccatgcc aagctccacg
    17641 ccattgaccg ggaggccccc gccgaggaac aggcggcccg gccagaccgc agcacccgcg
    17701 aggtacaggc cgaaagcgtg gcctataccg tctgccagta ttacgggctg gacacgtcgg
    17761 actattcttt tggctatgtg gccgattgga gcagcggcag ggagttatcc gagctgaaag
    17821 cctcccttga aaccatccgg gccacggcca gcgaattgat aaccgagatc gacggccatt
    17881 ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca ggaggccgtc acggttgacg
    17941 ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga acaggccccg gcccttgatc
    18001 cggcagccga gccgcttgtg actgtgctat ggagcgaaag cccccagcta aaggacggcc
    18061 agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg tctggatgca gcatatccgg
    18121 cagaggcagg ctatgacaaa accgcgttcc gcattgactg tatctttcag ggcgagccgg
    18181 acagctacga ggggcggcag gattttggag atagggacgg ttccctgatc gaacatatcc
    18241 agagctacca ccaattttac gcgcaggacg aaaactggaa aaagcatgta atcgctacaa
    18301 ccggcataga gggctgggag caggacaagg cccatcggga tatgctgtta ggcgagtttg
    18361 tcccctatat gaagctgcat tgcagccttg cccaaatgga acagacggcg cagggccgct
    18421 tacagtccgg ggaaaccctg acgccggagg aaacggccta tttgggggcg cttctctctt
    18481 atgtgcagga gtgccgcccc atgctcaatc agggccagta tcagttgccg gagccgccga
    18541 aactggctga cttcgaccag agcttgcagg actacaaagc acaggtacag gccgagatcg
    18601 cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata cgcggcggct ggctatgaag
    18661 caccccaaca ggacaccttt tccatctacc agcttcgagg cggggaggaa acccgcgact
    18721 atcggtttga gccatacgag cgtttacagg ccgcagggct ggccgtggac agtgccaact
    18781 atgaccttgt ttatacggca ccgctggata aaagcacgtc tttggaggat atttaccgca
    18841 cattcaacat cgaccacccg gcagatttta ggggacacag cctttccgtt tccgacgtgg
    18901 tggtgctgca ccagaatgga caggacaccg cccactatgt agacagcttc ggctatatgg
    18961 aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc cgaactgtct acggagcaga
    19021 acgaaaacat gattgacggc attatcaaca atacgccgcc cgaaccgcag ccggaggaac
    19081 ccgaacagct atcttttgag gatgtggcag ttgcttccgg ccagacacag gaggaacccc
    19141 cggaggctgc gcccccgcaa acggctgtcc gctattatcc catcaatgag ggagccgccc
    19201 gccgcgccaa ggaagccatg agcttttctg actaccggcc cggcagcgcc acggcggaat
    19261 accggcacta tgtcgatcag gccgtggaaa ttgcggagcg ccagaaaaag cgtgtggagc
    19321 cagagtatca cgaaaagata gacagcctcc ttgacaccta cgcccgcaag ctggccgaga
    19381 acatgaataa gggctatgag attaccgccc gcgtcccctc tgtcctgatt gccgggccgt
    19441 ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc cagtgaccgc aacatggagg
    19501 aatttcagta cattcagggc ttgcttgaca aaatccgcag caccggcatg ggtggtatca
    19561 gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa actggaaaag ctggaagcgt
    19621 cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa gcatgggacg ctggacggat
    19681 gcccccatct gaccgaaaga ggcattgaaa acctgaaagc ggatatggcg tcgggctggc
    19741 actatgagaa aaaaccgttc cagtcctggc agctctccaa caataacgcc gagatacgcc
    19801 ggttaaaggg ccgcattgag gaattgaccc gccaaaagga ggcggcctat gtgggatggg
    19861 agtttgacgg cggcactgtg gagatcaacc gggtggccaa ccgcctgcaa atcttctttg
    19921 aggggaagcc ggatgctgct gtacgcgacg agctgaaaag caacggtttc cgctggtcgc
    19981 ccaaggccgg ggcatggcag cggcagttaa acgacaccac aatccgcgtc gctgaccgta
    20041 tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc cttacagctt gcggcccgca
    20101 gggagaaaga aaagccctct atccgggcgc agctcaacgc ggccaagacc gaacaggcca
    20161 agcagccacc ggcgcgggaa aaatcaaagg gattggaggt agagtaaatg cagaagtttg
    20221 aaaatgtgga cgttctggcc gcgctggaac agatcatgcg gcagaacacc gcgttttatc
    20281 aaagtgactt tggcattgac aaagacatta tcaggcggga tgccgccagc gatcaggccg
    20341 cagacaagac cctgctctgg atgtcccggc cctccgggac gtactgtttc cgggaacgtg
    20401 atgttttcct gaaagacacc cgacagtaca acacatggaa attttacggg gagcagaccc
    20461 gtgacaagat actggcctac gcggtggagc tgaccggcac acagggcggc actatccggg
    20521 gcaatctcta tgagctggac tatccgcagc atttccggca cgtcattgaa accgcccagc
    20581 cggtgagtgt gaaccggctc ttttatgagc atggcacccg tgacatcccg gagagccagc
    20641 gttttgacgg aagccctgac cgggtattgg gaaacttttt gtactatgag gcccagcccc
    20701 atgacccggc tgtgctgcag gaggcgctac ggacggagca gcacagccgg gggcgggccg
    20761 tccccggcga tttcaaagtc cacgtcaccg ctttgcatga cagccggatt gaaaccgagg
    20821 cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc taacagcccc aataaaacgc
    20881 atttcatggt ggagatctcc ccgcactttg cggcgattgc ttccaccaag gataatgacc
    20941 ggctctttgc catgctgccc tataaatccc tgtgttttac cgggatgaaa ggcaggcatg
    21001 gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt gagtattcgc aggccccggc
    21061 cctctatccg caagcagcta tccgacagca aacaggccgc cagcccaaaa aaggcggcgg
    21121 ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat gcactttacc gtggaggaag
    21181 aaaacctgat ctgtatgtac cacaatgcag accggcgcag aacgataaac aggctcaccg
    21241 ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc ccagcagacc ctttccaagc
    21301 tggaacgcat gacggatgcc gactatgacg gccagaagtt ccacttcaca gacgaataac
    21361 gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg cgtccggcgt cttttctttt
    21421 tgcctattcc tacgcattta gaacccggta ttgtcgggga taggatgaag tattcgcttg
    21481 cccctgcaaa catgcgggag aagccttgca gcgggcggtt ttcaatccct taaatgcgta
    21541 ggttgcagct ataattagac ccctgaaaca gatatagccg cgcagcgggt gtatctgttt
    21601 caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg gtgcagccct cttgccgtct
    21661 atctctgttc cagcgggaag ctcggcaatg cttccagcag tttcagggaa atgcgctgcc
    21721 gtatatcttc gtcaatgact ttcttctttt tgccgtttgc ctgtctgatc tccaccgtgg
    21781 aaagctcgtt gatgtagtca gcataatggg ccaacacttt ttccgtggcc cacctctcac
    21841 cggcaacggc ggctttgatc gtttcatagt ccagcagctt ttcttgtccg tccatcctga
    21901 tacccccgtc agactgattt tttctatgat accatattcg gaagcatgga accagagaaa
    21961 acggcgcttt tctgctggta aattctacgc atttagaaca gcacttcggc ggggatagga
    22021 taaaacaccc acataccccg ctgaccatgc cggaaaagcc ttgaaatata agggctttta
    22081 cagctctaag tgcgtaggtt tccagtatct tttccgctgg cgctcggcct ctttgcggcg
    22141 gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg ttagacttcg gcgtgaacgt
    22201 ggccccgcac tcacagcagc gtttcacttc gtcccggtct ttggtgatct cggcatacag
    22261 ggccgcatcc agcgggagga cagcggcctt aaaccagcgg cacagaagcg aataggaaat
    22321 gctctgtacg caaatacatt cttcgccgtc gtccagcaac aggcagttgc cgtcacaata
    22381 gttgcagcac tcatgcgtca gcttccgggc cttgcgatat tgagggtaat tcattcgtgg
    22441 aatactcata gctcttgcgt ccattccttt cccggcgtca tacgcaaaat gccgtccaca
    22501 ttctgcttga tgatgccgta ttcctgcatc tgctttttcg cttcgccgta cttctcatac
    22561 agccgttcct tttcctcggc caatcgcttg tactcggctt tcagggcgta cagatcgggg
    22621 agctttttca caccctgtcc ctttagggcc tttgccgcag cttcatacaa aatcaacgtg
    22681 ctttcatgct cacgccggaa ttttgcctta tccgctgcat tgcggtattc catagccacg
    22741 ggccagagct gcttgtaggt agaaatattc tttatcagca ccgccatatc cgcaagccgg
    22801 tgttccatag ctttgagggt agcggcggcc tcgtcgctgg cggcggtgat ctcggccact
    22861 ttgctttcca aatccgggta ggtgtctatc ccatgttcag tcaggaaatt caaagtcttt
    22921 gcggcctgct tcaaattgtg gagcttcgcc cacctctcat agcctgccga ctgctgggcc
    22981 ttaatgttgt tttccagatc aatccgcaaa gaaatcccct tgcgctcttt gggcgctctg
    23041 gtgataacgc ggcctttgat acgctctttg atggcttcct cggtgtaggc ttcccccaat
    23101 gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga aagaaataaa ctttcccggc
    23161 ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat catcaaaatc ttttgcctgc
    23221 gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc agctcgtccc tttccgctgg
    23281 gcgtcccact cggcatagct cttgccctta ctctggccgg gcttcaccac ggacaatccg
    23341 ttttccttgc acagcctgtc gctggtgcgc cggatatagg catagctctg tttgttggaa
    23401 atgtacttgc gctgatttac catatccacg gcacagaaaa taatgtgatt gtggacgtgg
    23461 cccttatcaa tatgggtagt cagcacatag ggatatttcc cttgcagcac ttcctcggca
    23521 agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt cgccgggggc aaaggattgt
    23581 atcaggtggt ggcccagatt attgcccttt tggtatgcct gcgccagcag catttcaaat
    23641 tcaatgtccg ctgtttcata agagcaggca aaggaggcca ccagcagctt gccgtcagtt
    23701 ttgtccgggt tctcgatgta gtcaagggct tttttcagcg ttgacttgat aggatgtatc
    23761 tttgtaactg ccatatctgc gccagcgccc cctttatatc ctcgatgtcc tgtgcgtaca
    23821 ccggccccat accgttcagc ctctttgcaa tctgattgac gttgacgccg atcttctgta
    23881 tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt gacgatatag ccgtcaatga
    23941 gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag cagcgccatt ttctgttcaa
    24001 tcatagcccg ttcctccggc gtcacaggga cgcgcagcac gatttttcgc tttctgtttg
    24061 ccatgcttca ccgtcctttc cattcagggg tttggggatt tccccaacaa gcaaaacgga
    24121 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
    24181 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
    24241 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
    24301 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
    24361 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
    24421 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
    24481 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
    24541 aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta ttttctctgc
    24601 cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg cgccgaaagt
    24661 ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta cattgtatat
    24721 ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat
    24781 tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg
    24841 gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac
    24901 tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt
    24961 cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa
    25021 tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt
    25081 ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat
    25141 tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat
    25201 catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt
    25261 atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg
    25321 gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg
    25381 tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta
    25441 actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc
    25501 ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt
    25561 acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc
    25621 cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg
    25681 gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat
    25741 cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta
    25801 ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa
    25861 gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg
    25921 aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt
    25981 ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca
    26041 ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac
    26101 acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg
    26161 cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac
    26221 gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa gccattcaag
    26281 atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag
    26341 gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt
    26401 acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa
    26461 ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagagt
    26521 gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc
    26581 atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg gaaagaaaag
    26641 ggattttcat taaggttttt tcctatacga tcattgtcct gctactgctt gtcggtgtaa
    26701 cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa ttacagcaaa
    26761 cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg cttgatattc
    26821 aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat attgaagata
    26881 aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt tttagacccg
    26941 actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa agcaaggcag
    27001 gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt gcgataatgg
    27061 ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag
    27121 ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc
    27181 gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc aggctgaaag
    27241 aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag gaaacacagc
    27301 gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct acaagcgttc
    27361 tgttggaggg aatgcttgaa aatatcggtg actacaaaga tcattctaag tatctgcgcg
    27421 aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg gagcttgtca
    27481 gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg cgcacggttg
    27541 ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag
    27601 atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag gcactatcca
    27661 atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg atatggagtg
    27721 agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac attgatgata
    27781 ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcga agcagaaaaa
    27841 gtgggcgaaa cggtttagga cttgccatcg tacaaaaaac gctggatgcc atgagcctcc
    27901 agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta ccgctcacat
    27961 caacattgta aatatttaaa acttaaatga ttttgaccga caggtataac cctgccggtc
    28021 tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag atgcgcttgc
    28081 cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg cattcgatca
    28141 aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata tgaaacaatc
    28201 cgtggaaaag cgggcttttc tatgtgcggt cattatttcg ttcacagtct gcacgctgtt
    28261 tttgggggtg gagactggct tccgtattgg aggcaacaca gataccgccc atccctgcaa
    28321 ctcatacagg cagcagcact gacgtagtgg agaatttgga ggaaaacgct cttgccaccg
    28381 ccaaagaaca gggagatgaa caggaatgga gcctgatttt agtgaacagg cagaacccca
    28441 tccccgcaca gtacgatgtg gagcttgagc aactatcaaa tggtgagcgg atagatattc
    28501 ggatttctcc ctatcttcaa gatttgtttg atgccgcaag aactgatgga gtttacccga
    28561 ttgtcgcatc cggataccga acaacagaaa aacagcaaga aattatggat gaaaaaattg
    28621 ccgaatataa ggcgaaaggc tacacctctg cacaggctaa agcggaagca gaaacttggg
    28681 tggccgtgcc gggaacgagc gagcatcagc ttggtcttgc tgtggatatc aatgcggacg
    28741 gaattcattc aacaggcaac gaggtttata gatggctgga tgaaaacagc tatcgttttg
    28801 gttttattcg tcgctaccca ccagacaaga cagagataac cggtgtgagc aacgagccgt
    28861 ggcattaccg atatgttggc atcgaagctg ccacagagat gtacaaccaa ggggtttgcc
    28921 ttgaggaata tttaaaacca gaaaaatgag aaaaggatgt tatgttatgg acagaaaaag
    28981 attgacacag cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt
    29041 ttatgcggga atgagatttg acggctgtcg ctatgcgcag acgatagggg aaaaatcgct
    29101 ttcccatttg ctctttgaaa cggattgcgc attatacaac cacaataccg gatttgacat
    29161 gatataccag gaaaacaagg tgttcaactt aaagctggca gcaaagacct taaacggctt
    29221 attgataaga ccgggggaaa ccttttcttt ctggtggctg gtacgccatg cggacaaaga
    29281 taccccctat aaagacggcc ttacggtgac caatggtaaa ctcaccacca tgtcgggcgg
    29341 cggtatgtgc cagatgagca atttactatt ttggatgttc ctgcatacgc cattgacaat
    29401 tatccagcgc agaggtcacg aagtaaagga gtttccagag ccaaacagtg acgagattaa
    29461 aggggtggat gcaaccattt cagagggctg gattgattta aaagtgcgaa acgataccga
    29521 ctgcacctac caaatatgag tgaccttaga tgatgagaaa atcatcggtc aggtgtccgc
    29581 cgacaaagag ccccaagcat tatacaaaat tacaaatggc agtatccagt atgtccgtga
    29641 aagtggcggg atttatgaat atgcccaggt taaacggatg caagttgcct taggtaccgg
    29701 ggaaataata gattgcaagc tgctttatac aaacaaatgc aaaatctgtt atcccctccc
    29761 ggaaagtgtg gatattcagt ctaatggtga taagtcaaga tgaaatttaa gattttc
//
LOCUS       DACCKU010000270        29817 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.028, whole genome shotgun sequence.
ACCESSION   DACCKU010000270 DACCKU010000000
VERSION     DACCKU010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 29817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.028"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..431
                     /locus_tag="IVN13_003083"
     CDS             <1..431
                     /locus_tag="IVN13_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP7013484.1"
                     /translation="DFELPANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVET
                     LIHSATTNSNGVIEYGESKSKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPE
                     KELAGVVAGGITFQEKKENVSNKSTVGVVNQFSQVKAILI"
     gene            459..914
                     /locus_tag="IVN13_003084"
     CDS             459..914
                     /locus_tag="IVN13_003084"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP7013485.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            911..1669
                     /locus_tag="IVN13_003085"
     CDS             911..1669
                     /locus_tag="IVN13_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP7013486.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            2170..3369
                     /locus_tag="IVN13_003086"
     CDS             2170..3369
                     /locus_tag="IVN13_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013487.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            3391..3603
                     /locus_tag="IVN13_003087"
     CDS             3391..3603
                     /locus_tag="IVN13_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP7013488.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            3678..4154
                     /locus_tag="IVN13_003088"
     CDS             3678..4154
                     /locus_tag="IVN13_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP7013489.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            4151..5959
                     /locus_tag="IVN13_003089"
     CDS             4151..5959
                     /locus_tag="IVN13_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP7013490.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            6036..6251
                     /locus_tag="IVN13_003090"
     CDS             6036..6251
                     /locus_tag="IVN13_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP7013491.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            6343..7206
                     /locus_tag="IVN13_003091"
     CDS             6343..7206
                     /locus_tag="IVN13_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013492.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            7237..7779
                     /locus_tag="IVN13_003092"
     CDS             7237..7779
                     /locus_tag="IVN13_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP7013493.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            7793..8215
                     /locus_tag="IVN13_003093"
     CDS             7793..8215
                     /locus_tag="IVN13_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7013494.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            8145..10544
                     /locus_tag="IVN13_003094"
     CDS             8145..10544
                     /locus_tag="IVN13_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP7013495.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            10576..12567
                     /locus_tag="IVN13_003095"
     CDS             10576..12567
                     /locus_tag="IVN13_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP7013496.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            12590..12841
                     /locus_tag="IVN13_003096"
     CDS             12590..12841
                     /locus_tag="IVN13_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP7013497.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            12831..14060
                     /locus_tag="IVN13_003097"
     CDS             12831..14060
                     /locus_tag="IVN13_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP7013498.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            14057..16138
                     /gene="topB"
                     /locus_tag="IVN13_003098"
     CDS             14057..16138
                     /gene="topB"
                     /locus_tag="IVN13_003098"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP7013499.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            16287..20207
                     /locus_tag="IVN13_003099"
     CDS             16287..20207
                     /locus_tag="IVN13_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP7013500.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            20208..21152
                     /locus_tag="IVN13_003100"
     CDS             20208..21152
                     /locus_tag="IVN13_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013501.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            21156..21359
                     /locus_tag="IVN13_003101"
     CDS             21156..21359
                     /locus_tag="IVN13_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013502.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(21659..21895)
                     /locus_tag="IVN13_003102"
     CDS             complement(21659..21895)
                     /locus_tag="IVN13_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013503.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(22078..22449)
                     /locus_tag="IVN13_003103"
     CDS             complement(22078..22449)
                     /locus_tag="IVN13_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013504.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(22446..23774)
                     /locus_tag="IVN13_003104"
     CDS             complement(22446..23774)
                     /locus_tag="IVN13_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP7013505.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(23735..24064)
                     /locus_tag="IVN13_003105"
     CDS             complement(23735..24064)
                     /locus_tag="IVN13_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP7013506.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(24322..24693)
                     /locus_tag="IVN13_003106"
     CDS             complement(24322..24693)
                     /locus_tag="IVN13_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7013507.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            24838..25023
                     /locus_tag="IVN13_003107"
     CDS             24838..25023
                     /locus_tag="IVN13_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP7013508.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            25535..25753
                     /locus_tag="IVN13_003108"
     CDS             25535..25753
                     /locus_tag="IVN13_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013509.1"
                     /translation="MRQERPEGVHTGPACVKSHTARCIRKKEAAHFAEAWGVPNKQNE
                     NAAGGQVVDQNIRKGYSDPLLASIIGMF"
     gene            25966..26625
                     /locus_tag="IVN13_003109"
     CDS             25966..26625
                     /locus_tag="IVN13_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance response
                     regulator transcription factor VanR"
                     /protein_id="HAP7013510.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDAEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            26628..27971
                     /gene="vanS"
                     /locus_tag="IVN13_003110"
     CDS             26628..27971
                     /gene="vanS"
                     /locus_tag="IVN13_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP7013511.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            28142..28949
                     /locus_tag="IVN13_003111"
                     /pseudo
     CDS             28142..28949
                     /locus_tag="IVN13_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-B"
     gene            28967..29803
                     /gene="vanW"
                     /locus_tag="IVN13_003112"
                     /pseudo
     CDS             28967..29803
                     /gene="vanW"
                     /locus_tag="IVN13_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT         7675 a         7670 c         7996 g         6476 t
ORIGIN      
        1 tagattttga attaccggct aatcagattg ataagaacaa aacatatata aatgtaagga
       61 tggagcctgg gaaaatacag gaaatcccct acacggtctt taaccgaaca gataaatcag
      121 taacagttga gacattgatc cactccgcta cgacgaattc aaacggtgtg atcgaatatg
      181 gagaatcaaa aagtaaattt gatgaaacat taagttatga tatgaaaaac ttagcaaaaa
      241 cagtgggaga aataactgtt cctcctcaaa gtgcggtcac tgagacactt tcactgaaag
      301 cgccagaaaa agagctggct ggtgtggtcg caggagggat cacgtttcaa gaaaaaaaag
      361 agaacgtttc taacaaaagt acagttggtg tcgtcaatca gttttctcaa gtgaaagcaa
      421 tcttgatttg aaatagcgaa aaagagtctt ccccagattt gaaattaaaa aaagcttatg
      481 cggatcaaag cgatggaaga aatgtgatta aggcgaatct tcaaaatcca aatgcttctt
      541 atatgttcgg cttgactaca tcggttgaaa tctacaaaaa agaaacaaaa gagccttacc
      601 tttcaataaa aaaagaaaaa atagatgtag cacccaatac aaattttgat ttacttgtcc
      661 cattagaagg aaaaaaatta gctccgggac attatcaagc ggttgtgaag ggtgcttgga
      721 aagaaaaacg ctggaatctc cataccgatt ttgaaataac tgatgatgca gcggaccaat
      781 tgaatgaaaa ggatattgat ttaaaaaatc agccagaaac atttgactgg cgatggctga
      841 tacttgttgg agtcattctt attggcttat ttgctggatt atttatttta aaagaaagaa
      901 ggaaaaagaa atgaaaaaaa caattgcatt aagtaccgtt ctagctggaa gtctattatt
      961 atgtacacaa agtgtttttg ctgagaataa gagctcagcc actacagaag gaacgatttc
     1021 tgtgaccaaa ccagatgctg caaatcctga agacgttgtt ccagttcctc ctgttgatcc
     1081 aaatgatcct gatactcctt ttacacctga cgaaccaaat cagggaacat ccggattact
     1141 gacgattgac caagctccag atcttgattt tggaacaatc aagttaggta cagcaaaaac
     1201 agtatatgct gcattacaaa gcggaacgga cagtactgga gcacaaacac aagtacagaa
     1261 tttcgtacaa gtaacggata aaagcggaaa ttatgccggt tggaaattat cagtcaaacg
     1321 aacagaattt attggtacaa catctgatac gaataaacta actggttcaa aggtgaaatt
     1381 caaaaacgcc gtgattcgaa atagcgcaac taatagcagt ggaatgccat ctaccgtttc
     1441 tggaactggc gctgaaggaa tcgaaatccc agcagatcag gcagtagatc tagtaactgc
     1501 aggagcaaat gaagggattg gcacatggat ttattcatta ggagagaatg tggaacaagg
     1561 gagacaatca gtggaattat ttgttcctgt atccaattat gctgtagaca actacgtttc
     1621 aacattcaca tggtcgttaa cagatgctcc tacagaagag gaagcgtaaa atcatatcaa
     1681 cgatttcagg gagtggagcc gattcaaggc ccactccctg aaatctgcta tgcttacgtc
     1741 tcagctctta ttcatcaact cgaagcagtt ttttggaagt catggaaacg ttcctttgtc
     1801 ttttcaaaga tctttcgacc aacgagtaag gagtcaccgt tgaaaaattc taacacataa
     1861 tgagcagaat caattttctg caattgcgat aagttgatac agtaagattt taaaagtaaa
     1921 aattttaggt tatcgctgaa agccccggaa atacgggcat ttcgcaggat atgagaactc
     1981 aatttactac caatttacta ctttcggatt gagccgcata tatgctgaac catactgcta
     2041 atacccgcca ccccaaatac ggcacatcat aaattgaata cgacaccgta aacgcggcgt
     2101 tcctctttcc cgtcatggga gaacaggacg ccgctttttt atgcccaaat agagaaagga
     2161 ggcgacgcca tgctatttac ttccggccct gaccgggcgt ttgaacggct gatgcaggaa
     2221 aagcccggtt tcgatcacta cgacggcggc ggggcggacg cgccggagga ttgcggcacc
     2281 tgccgatttt accgcccgga ttggaaatat caattctgcc agtttgcaga gtgtccctat
     2341 cagccgggca agctgaccga ctatgcagcg gtcaaattca cagtgaaagg agcgaacaac
     2401 gctatggcag ttttccgtgt ggaaaagaac aaaggctata cggtgatgtc aaatcaccac
     2461 ctgcggaata agaacctgac cttaaaggcc aagggcctgc tgtcgcagat gttgtccttg
     2521 ccggaggatt gggactatac cctaaaagga ctgtcctata tcaaccggga aagcatcgac
     2581 gcgatcagga cagcggtatg ggagcttgaa aaagctggat atatccggcg tgagcagggc
     2641 cgtgacgcaa aaggcaaaat ggccgatatg gtctatacca tctacgaaca gccggtattg
     2701 gaaaacccgg tattggaaaa tccaacatcg gataacccgg tgttggaaaa tccgacatcg
     2761 gataatccgg cgtcggaaaa tccaacgcaa ttaaataaag aagtacaaag aactgactta
     2821 tcaaaaaaag aaaaaataat tacagatgga caaagtaccc attccattcc tatcccatcc
     2881 ccgacccctg cccctatgga gggcgaggca gcggctacgc cgccggaacg gaaaaggaca
     2941 ggaaaggatg aggctgtacg gatttatcgg gaaatcattt tggagaacat cgagtatgcc
     3001 tatctcattc aggacaggag cattgaccgt gaacagcttg acgaaatcgt agacctgatg
     3061 ctggaaaccg tctgcaccgc ccgaaagaca atccgtgtcg ccggggacga ctaccctgcc
     3121 gagctggtga aatccaagta catgaagctg accggcgaac atatccgctt tgtccttgac
     3181 tgtctgcggg agaacacaac gaaagtccgc aatatcaaac aatacctgcg ggctatgctg
     3241 ttcaacgccc ccagcaccat ttccaactac tacacggccc ttgtcgcgca cgacatggca
     3301 cagcccgatt ggggaaagcc taaatccggc ctgccggact attcctgcgg cgagggcgaa
     3361 agcctgtaac gagaaaagga ggacgaccct atgaagcagg gcgcattgat ttttgacgag
     3421 cgaacagacc gttacgacat ccgctttgat ctggccgact attacggcgg cctgcattgc
     3481 ggggagtgtt tcgacgtgat gatcggcggc aaatggaggc ccacccgcat tgaaatggcc
     3541 gaaaactggt atcttgtcgg catccgggcc gacgaccttt ccggcctgcg ggtgcggatt
     3601 tgacctatat ctacctgcgg cgtctggtgg ttccgcctta gacgccgctt ttgactttct
     3661 gacaaggagg gatgcgattg caggaagaag ttactcaaaa gaccattgcc ctgtccatga
     3721 aaacgggcaa gctcacggct caagtgctgc aagcggcgct gaaaaagtac ctgcaacacc
     3781 gggccaaggg caaaaacacg ctgcaccacg gccagcagag cctaaagcag cttaaaaagc
     3841 atggggctga tctctccaac attgagatca ccgaggccaa cattggagcg ttcaagccat
     3901 gcgccaagaa atacggcgtg gattttacct tacgaaaaga taagaccacc cagccgcccc
     3961 actacgttgt gattttcaag gccaaggatg cagataacct tgatctggcg tttcgggagt
     4021 ttaccgccaa gacgctttcc aaggagcagc ggccctctat ccgcaaggtt ttgtccgctg
     4081 tgaagcaaaa ggcggcggcc cagaccaagc agcgggccaa ggagaaaatc aaggaaaggg
     4141 ggctggaact gtgaagcctg acgtaaagaa attgctgatc ctgaacctcc cgtatctgat
     4201 ttttgtgtac ctgtttgcga aatgcgggga ggcgtaccgg ctggccgctg gtgctgacct
     4261 gtcggggaag ctcctgcatt ttgcggatgg ttttgcggcg gcttttgcca acccgctgcc
     4321 aagcctgcac ctgtttgacc tgtgtatcgg tatcgccggt gccgtgatcg tgcggctggt
     4381 ggtgtactgc aagagcaaga acgtgaagaa ataccgcaaa ggcatggagt atggcagcgc
     4441 ccgctggggg acggccaaag acatagcgcc ttacatcgac cccaagtttg aaaataatat
     4501 cctgctcacc caaacggaac ggcttaccat gaccggcagg cccaaagacc ccaagacagc
     4561 gcggaataaa aacgtgctgg tgatcggtgg cagcggttca gggaaaacga gatttttcgt
     4621 gaaacccaac ctgatgcaat gctttccgac cacggactat ccgacttcgt ttgtggtgac
     4681 agatccgaaa ggcactttgg ttctggaaac cggcaggatg ttccagcagg cgggctaccg
     4741 cattaaaatc ctgaatacca ttaacttttc caagtccatg aagtacaacc cctttgtcta
     4801 catccattcg gaaaaggacg tgctaaagct ggtaaatacc ctgatttcca acaccaaggg
     4861 cgagggggaa aagtcagcgg aggatttttg ggtgaagtcg gaacggttgt tttattcggc
     4921 cctgatcggc tatatctggt acgaggcccc agcggaggaa atgaacttct caacgctgct
     4981 ggaaatgata aacgccagtg aagcccgcga ggacgacccg gagtttcaaa gccccgtcga
     5041 tctcatgttt gagcgtttgg agcagaaaga ccccgaccat tttgcggtgc gccagtacaa
     5101 gaaattcctg ctgtccgcag gcaagacccg ttcttccatc ctgatttcat gcggtgccag
     5161 acttgcgccc tttgacatcc gggaggtacg cgagctgatg gaggacgacg aaatggagct
     5221 ggacaccatc ggggacgaaa agacggtgct gtatctcatt atgagcgata cggacacgac
     5281 ctttaatttc attctggcta tgctccaaag ccagctaatc aacctgcttt gtgaccgggc
     5341 tgatgatgtg tacggcgggc ggctgcctgt ccatgtacgc ctgattttgg atgaatttgc
     5401 caacattggc caaataccca atttcgataa actcattgcc accattcgaa gccgtgaaat
     5461 atcggcctct attattttgc agagccagtc gcagttaaaa gcgatttaca aggacgccgc
     5521 cgagatcatt tcggataact gcgatagtgt gcttttcctt agtgggcgcg gcaaaaatgc
     5581 caaggagatt tccgatgcgc tggggaaaga aacgattgac agtttcaaca ccagcgagaa
     5641 ccggggaagc caaacgtccc acggattgaa ctatcaaaaa ttaggaaagg agttgatgtc
     5701 ccaagacgaa attgccgtga tggacggcgg caagtgtatc ttgcagctac ggggtgtgcg
     5761 cccgttttta tcggagaaat tcgatattac caagcatccc cggtacaaat accttgccga
     5821 cgccgacaag aagaatacct ttgacgtgga cagatttttg actatcacgc gccggaaacg
     5881 gcagcaagtg gtaacgcagg atgaagtatt cgacctgtat gaaatcgacc tgtcggacga
     5941 ggacgccgcc gctgaataaa cagcggcttt tttcattcca acaacaaaat caaaaaatca
     6001 ttatgagccg gaaaccggca gaaagaggta aatttatggc attttttaat tccgcagtta
     6061 ctactttgca gactatcgtg atcggactgg gcggcgctct gtgcgtatgg ggcggcatca
     6121 atctgttgga gggatacggg caggacaatc cgggggcaaa atcgcagggt gtcaagcagc
     6181 ttgtcgctgg cggcggcgtg gccctgatcg gtgtcactct ggttcccctg ctgtccgggc
     6241 tgctgggctg atcctggcct ttgtccctgt ccaaaccctt aactaatcgc ctccgcgtcc
     6301 tcccggccag accgggaggg cgcgggaaag gaggtgctac ccgtgattag tgaaatcatt
     6361 gaagaatgga taaaaggcat cctcatggac ggtatcacgg gcaacctgtc cgggctgttc
     6421 gatacggtaa atgccaaagt cggagaaatc gccgccgacg tgggacgaac cccgcaggca
     6481 tggaacagcg gtattttcaa tatgctgcac agcctgtcgg aaaccgtgat cgtccccata
     6541 gccggggcca tactcgccct tgtcatgtgc tatgagctta tccagatgat aacggaaaag
     6601 aacaatatgc acgactttga cagctccatg ttcttccgct ggatattcaa atccgcgttt
     6661 gcaatcctga tcgtaacgaa cacatggaac atcgtcatgg gcgtattcga cgccacccaa
     6721 agcgtcgtca atcaaagcgc cggtgtgatt atcgggcaga catccattga ttttaacacc
     6781 ctgataccag accttgaaag ccaattggag gctatgagcg tgggcgctct tttggggctg
     6841 tggttccaaa cccttgttgt gggcctcacc atgaatatcc tttccatctg cattttcctt
     6901 gtgacctatg gccgcatggt ggaaatctat gttgtcacgt cgctggggcc tatccccctt
     6961 gccacaatgg gcaacgcgga atggcgcggc atgggccaag ggtatctgaa atccctgctg
     7021 gcgctgggct tccaagcctt tttaatcatg gttgtggtgg gtatctacgc ggtgctgata
     7081 cagaatatcg gttccgcttc cgacatatcc ggcgcaatct ggggctgtat gggctatacg
     7141 gtgctgctgt gcttctgcct gttcaagacc ggcagtatat caaaggccgt attcggggcg
     7201 cattgatccc aaaatccaag catacacagg aggaacttgt ttatttcaac gtacaacatc
     7261 atttacgctg acccgccgtg gcggtatgac tgcaaaaacg tacagggcgc agcggaaaat
     7321 cactattcca ccatgagcat tgacgagctg tgcgccctgc cggtggaacg gctggcgtcc
     7381 aaagactgcc tgctgttttt gtgggccaca ttccccatgc tgccggaagc tctgcggtta
     7441 atcaaagcgt ggggcttttc attcaagacc gtggcttttg tatggctgaa acagaaccgt
     7501 aaaagcctta catggtttta cgggctgggc cgctggacaa ggggaaacgc ggaaatctgc
     7561 ctgctggcaa cacacggcaa gccgaagcgt cgttctgcct ctgtccatca gtttattatc
     7621 agcccgattg aacaacacag caaaaagccg gatgtgaccc gtgaaaaaat cgtagagctg
     7681 gcgggcgatt tgccccgcgt ggagctgttt gccagacaga aaacccccgg ctgggacgtg
     7741 tgggggaacg aggtggaatg tgaccttatc ctgacgtgaa aggaggtgcc aaatggctta
     7801 tgtaaccgtc cccaaggact taacccatgt gaaaagcaag gttgtgtttg ggcttaccaa
     7861 acggcaactg atctgttttt ccggggcgct ggtgatcggc gtccccttat attttctcac
     7921 ccgtgactat cttaccaaca gcgcggcggc cctgctcatg gtatttgcca tgctgccggg
     7981 ctttttgctt gccatgtacg agcgtcacgg ccagcccctt gaagtggtga ttatgcagat
     8041 catccagtgc tgcttcatcc agccaaagga gcgtccctat cagaccgaca acacctacac
     8101 cgcccttgtg cggcaatacc agatggaaca ggaggtaaag gccattgtcc aaaaaggaaa
     8161 tcccaagaga aaccgaaaaa acaaagctga cccgcgccca gcgaaaagaa attgacgcgg
     8221 taatccgtaa atataagggc gacggcaggc cccacacggc gcagcagagc atcccctacg
     8281 aggtcatgta cccggacggc gtgtgccggg tatcgcccgg cgtcttttcc aagtgcattg
     8341 agtttgcgga catcagctat cagcttgccc agccggacac gcagacggct atctttgaaa
     8401 aactgtgcga cctgtataac tatgtggacg cctccattca tatccagttt tctttcctca
     8461 atcggaaagt tgacccggtg cagtacgcca agagctttga gatcgcgccg cagggggacg
     8521 acttcgacga catccgggcc gagtacaccg gcattctgca aaagcagctt gcaaacggca
     8581 acaacggcat ggtaaagacc aaatacctga cattcaccat cgaggccgaa agcgtgaaag
     8641 cggcgcgagc cagattgaag cgcatcggct ttgacctgtt aggctatttc aagagcatgg
     8701 gggccgtagc ccatgtgatg gacggctggg aacggctgaa cctgctgcat ggcgtgtacc
     8761 acccggacgg cgagatattc aattttgact ggaagtggct ggccccgtcc ggcctctcca
     8821 ccaaggattt tattgccccg tcctccctgt gcttcggcaa tgcaaagaca ttcggcatgg
     8881 gcggcaaata cggcgcggta agtttcctgc aaatcctgtc cccggagctg tcggacgata
     8941 tgctggccga cttcctcaat acggaaagcg gtgttttagt caatctccat gtgcaggcca
     9001 ttgaacagac caaggcgatc aagaccatca agcgcaaaat taccgacctt gacgctatga
     9061 agattgcgga gcagaaaaag gccgtccgca gcggttacga tatggacatt cttcccagcg
     9121 accttgccac ctacggcgag gacgccaaga agctgctgac aaagttgcag acccgcaatg
     9181 aacggctgtt ccagttgacg ttccttgtcc tcaacgtggc cgacaccaag caaaagctga
     9241 ataacgacgt gttccaagcc gcaggcgtgg cccaaaagca taactgtccc cttgtccggc
     9301 tggactacca gcaggaacag ggccttgcaa gcagcctgcc gctgggcgtc aatcagatca
     9361 agatacagcg cagccttacg acttccagcg tggccgtgtt cgtccccttt gtgacgcagg
     9421 agctttttca gggcggcgcg gccatgtatt acggcatcaa cgccaagtcc cgcaacatga
     9481 ttatgctgga caggaagcag gcccgttgcc ccaatgcctt gaagctcggc acccccggca
     9541 gcggcaaatc catgagctgt aaatccgaga tcgtcagtgt gttcttaacg acgcctgacg
     9601 atatttttat ttcagacccg gaggccgaat attaccccct tgtcaaacgg ctccacggac
     9661 aggtaatccg gctgtcgcct accagcaagg actttgtgaa cccgctggac atcaacctga
     9721 attattcgga ggacgataac ccgctggccc tgaaatccga ctttgtgctg tcgttctgtg
     9781 agcttgtcat gggcggcaag aacgggctgg aagccattga aaagaccgtg atagaccggg
     9841 ctgtgcgcgt gatctaccgg ccctatctgg cagacccccg cccggagaat atgccgatcc
     9901 tgtccgacct ccacaaagcg ttgttagatc agcatgtccc ggaggctgac cgggtggcgc
     9961 aggcccttga cctgtatgtg tccggctccc tgaacgtatt taaccaccgt accaacgtgg
    10021 acatcggcaa ccggcttgtg tcctttgaca ttaaggagct gggcaagcag ctcaaaaagc
    10081 tggggatgct cattgtccaa gaccagatat gggggcgcgt cacggccaac cgaagccaag
    10141 gaaaggccac atggtatttt gccgacgagt tccatttgct tttgaaagag gaacagacgg
    10201 cggcctattc tgcggagatt tggaagcggt ttaggaaatg gggcggcatt ccaacaggtg
    10261 ccacccagaa cgtcaaagac ctgctttcct ccccggaaat tgaaaatatc cttgaaaaca
    10321 gcgacttcat tacgctgctc aatcaggcgt ccggggatag aaaaatcctt gcggaacgct
    10381 tgaatttgtc caccgaacag cagaagtaca tcgacaattc cgaacccggc gagggccttt
    10441 tgatctttga aaacgtggtg ctgcccttta ccaaccccat cccgcacaac acccagcttt
    10501 acaagattat gacgacccgc ttaaacgagg tggcgggagt atgacccggc gacgccacaa
    10561 aggaggtgaa ccaccatgaa agagctgaaa gccaaggcga aagtgacgca ggccatgacc
    10621 cgcgacgggc tgacgctgga aaatcaggcc accggcgagg tggagaacat ttccagccgg
    10681 gaggccgaac aggatttgtc cgcgccgggg ccgggcggca ccgccgataa gctgctggaa
    10741 cgggccgacg ccatccatga cggccataag gccaagaaag ccgcgaagca ggccggggct
    10801 gtggccgccg agggggacgc tgccctgcac cggccatccg cccggctcca actgtcggag
    10861 gaagaacggg ccgacccgga tttacagccc tatatccgaa aatcggacaa acgggccgat
    10921 aagctggata aggcccgcgc cgccatcccc aagaagcgcg tcccggtcaa ggaaaccgtc
    10981 tttgacgagg caagcggcaa agcaaagacc gtcctgcact ttgacaaaca ggacaagggg
    11041 cctccctccc tgaaacccaa tccggcaggc cgtcctgttt ccgaggtttt tctattcggc
    11101 cacgggaaaa tccatgaagt cgagcatgaa aacgtgggtg tggagggcgg ccataagggg
    11161 gaggaacttg ccgagcggca ggccggaaaa gccatccgca gcaccatccg gcaccacagg
    11221 ctgaaaccct actgggacgc ggaaaaggcc gagcgcaggc tattgtctgc caacgcggag
    11281 tatttctacc agaaatcttt gcgggataac ccggagatcg cacaggcggc cagcaacccc
    11341 atttcccggt tctggcagaa acagcagatc aagcgccagt acgccaaggc cgcccggaca
    11401 gcgggccaga ccgcaggcgc ggcggcacag ggcgcggcca agacagccgg ggcctccacg
    11461 aaagcggcca aggccgcagc caaagaaagt gaacgggcgg cctcctttgt ggcccgccac
    11521 tggaaagggg cgctggccgt ccttgtgttg gggcttttga tgctgcttat catcggcggc
    11581 ttacagtcct gcaccgccat gttcggcagc gccggaagcg gcatcacggc gtcctcttac
    11641 ttttccgagg atagcgatat gttggccgcc gaggacgcct acgcggagct ggaagccgag
    11701 ctgcaagact atctggacaa ctacgaggcc acccatgact atgacgaata ccattttgac
    11761 cttgacgaga tcagccatga cccctatgtg ctgatttcca ttctgtcggc gctccatgac
    11821 gggatattta ccatcgggga ggtacagggt gatttgtcca tgctctttga caagcaatat
    11881 atcctgacag aaaacgtcgt ggtagaaacc cgttaccgca ccgaaaccga tacatggacg
    11941 gacgcggagg gcaacaccca tacagacacc taccaagtgc cgtatgacta ttacatctgc
    12001 tacgtcacgc tggaaaactt cgacctgtcc catgtgccag tctatatcat ggacgaggaa
    12061 acgctaagtt tctattccct gtatatgtcc acgctaggaa accggcctga tctgttttcc
    12121 ggcagccagt accccaacgc ctccaccctg acgccgccga cctactacga cgtacccccg
    12181 gaggctttgg aggatgccac ctttgccgcc atgctcaaag aggcggaaaa atatatcggt
    12241 tatccctatg tgtggggcgg cagctctccc agcaccagct ttgattgcag cggcttcatt
    12301 tcgtgggtaa tcaatcatag tggatggaac gtgggacggc tgggggcgca gggcctatat
    12361 aacatctgca cccctgtttc cagttcgcag gccaagcccg gcgaccttgt atttttcaag
    12421 ggaacctacg acacccccgg cgtgtcccac gtcggcctgt atgtgggcaa caatgtgatg
    12481 ctgcattgcg ggaacccgat ttcctacacg aaccttactt caaactactg gcaatcccat
    12541 ttttacagtt acgggcgttt accttaacgc cagaaaggag ctttcaatta tggcaaccaa
    12601 acttgaccgc atcgaaaagg acatccagaa aaccaagtct aagatcgcgg agtttcagaa
    12661 acagcttcgg gagctggaaa cgcagaaaac cgaacaggag aatttgcaga tcatccagct
    12721 tgtgcgcggc atgaacatga agccggagga atttgccgcg ttcttacgca gcggcgcaat
    12781 gcaggccgcc cctgccgcaa ccccttacca tgaacaggag gacaatgccc atgaagaata
    12841 agaccagacg gatttcccgt attctccccg cacttctggc cgtgatgctc tgcgtcacgg
    12901 cctttcccgt taccgccctt gcaggcggca atgacccggc accggccccg ctgccggagg
    12961 ctacaaccga acccgctacg ggcggtttgg agcctgaaac agacgaaacc ggcaccgcca
    13021 cggacgggac agaaaccgag gccgaaaaag ataccggccc tctgaccggc aaggacatca
    13081 ccgacctgtt ttccgcgctg ttcggttcca aggtgagcat tgccgccacg gacgacggca
    13141 tccagatcac caccggcacc gacaaaaaag aacctgaaca gaccggcacc gtcaccacca
    13201 acggcggcag gctgaatgtc cgcaccggcg cggggcttga caagaccgcg ttcacccagc
    13261 ttcccaatgg gacgacggtt gaggtagtcg gcacagacgg cgactggatt aaaattctct
    13321 tgccggagcg catcggctat gtccattccg actatatgac cgtcagcgaa aaggaagtag
    13381 cggctaccgg cgagggcggt ttttccctct ccattgaccc ggaggaaatt gcttctctgc
    13441 tggaactgtt caatggcggc ggggccagcg ccgccctgtc cccggacggg aacctgtcac
    13501 tgattgacga catcggcagc gccgcgaaaa atggcaagca gtttatcacc gtggaaacca
    13561 aaaacggcaa tgtgttttat ctgattattg accgggacga cgagggaaaa gaaactgtcc
    13621 atttcttaaa tcaggtggat gaagccgatc tgctgacgct gatggaggac ggggaaaccg
    13681 tcacccccgc tgctgtctgc tcctgtacca cgaaatgcaa ggccggggct gtgaacacga
    13741 attgcccggt atgtaaaacc aacctgaccg agtgcagcgg cccggagccg caggaacccc
    13801 agccggagga acccgaagcc ccggaggaag aacccaaggg cggcgcgggc ggcctgatcg
    13861 tgttcctgct ggtggcgctg gcgggcggcg gtgcggccct ttactatttc aagttcaaaa
    13921 agcccaaggc tgaaaccacc ggcagcgacg atttgggcga gtatgatttt ggggaggacg
    13981 aggatttgga ggacgaggaa cccgaaaccg agtttgaccc ggaagctgat tttgtgagtg
    14041 aacaggagga cgaggaatga catataaact tgtaatcgcg gaaaaaccca gcgtggcccg
    14101 cagcattgcg ggcgtgatcg gggccacgga gaaacacgac ggctatttgg aggggaacgg
    14161 ctatctggtg agctggtgca tcggacatct ggtgtccttt gccgacgccg gacgctatga
    14221 cgagcgtttc aaaaaatggc gctatgagga tttgcccatt ctcccggaaa catggcagta
    14281 catcgtcccc gacgacaaga aacagcagtt tgacgttctc tgttcgctga tgcagcgccc
    14341 cgacgtgacc ggccttgtgt gcgccaccga cgccgggcgc gagggggaat tgattttccg
    14401 tttcgtctac cagatggcgg gatgccaaaa gccctttgag cgcctttgga tttcctctat
    14461 ggaggacagt gctattaagg acggctttgc ccacctgaaa ccgggcggcg actatgatcc
    14521 gctgtatcaa tcggccctgt gccgggccaa ggccgactgg ctgatcggca ttaacgccac
    14581 ccggcttttt tccgtcctgt accacaagaa cctgactgtg ggccgcgtcc agacgccgac
    14641 cttaaaaatg ctggtggaca gggacgccaa gattaccgat ttcaagaaag agaaatatca
    14701 catcgtccat atcacggcgg gcggcgcgga cgcggtaagc agccgctttt ccgacgctgc
    14761 cgaggcaaac accgtcaagg cggcttgtgc aaaagcgcag gccgtttgcg tttccgtgac
    14821 gcgggagaaa aagacggaac agccgcccaa gctctatgac cttaccacct tgcagcggga
    14881 ggctaaccgg cttttcggct ataccgccaa gcagaccctt gactatgccc aggcactgta
    14941 tctaaagaag ctgctgacct atccgagaac ggatagtcaa tatcttaccg acgatatgct
    15001 gcccgccgtg gaaagccttg tgtccggcct gtgggagctg gtgccgtttg ccaagggcct
    15061 gaacctttcc ccgcagtttg accgcatcct gaacagcaaa aaggtgtccg accaccacgc
    15121 cattatcccc accgctgaat ttgtgaagca gggctttggc actctggccg agagtgaaaa
    15181 aaagctgctg tccctgatct gctgcaagct cttatgtgcc gtggccgggc cgcatatcta
    15241 tgaggccgtc accgccacat ttacctgtgc cgggcaggag tttaccgcca agggcaagac
    15301 cgtcctttcc cccggctgga aagagattga acgccggttc cgttcttcct taaagaccga
    15361 cgccgacgag gacgccgagg cggtgcggga gctgccggag ctttgcgagg gccagacctt
    15421 tgcagacgtg gccgcctctg tcaccgagca tttcaccacg ccgcccaagc cctacaccga
    15481 agatacgctt ttgtccgcta tggaacgggc cggggccgag gatatgccgg aggatgccga
    15541 gcgcaaggga ttaggcacac cggccacccg cgccgccatt ctggaaaagc tggtgcagat
    15601 gggctttgtc cagagaaaag gcaagcagct cattcccacc aaggacggca tcaatctggc
    15661 tgtggtgctg ccggaggcgc ttacttctcc ccagcttact gccgaatggg aaaaccggct
    15721 gaccgagatc gccaaggggc aggccgatcc tgacgaattt atggacggca tcgagacgca
    15781 ggcgcgggag ctggtgcaga cctattcctg tatcagtgag gaaaagcaga aattgtttca
    15841 ggccgagcgt gttcctatcg ggacgtgtcc ccgctgtggt gaggctgtct atgagggcaa
    15901 gaaaaactac tactgcggaa accgggcctg ccagttcgtc atgtggaaga atgaccgatt
    15961 ttttgaggaa cgcaagaaag cgttcacccc gaagatcgcc gctgcgctgc tcaaaaccgg
    16021 caaagtcaag gtaaagggcc tctattccgt aaagaccggc aaaacctacg acgggacggt
    16081 gcttttggct gataccgggg gcaagtacgt caactaccgc attgaacaca ggagctaaag
    16141 gacagtaaca cagcaagcat agggagtgtg gctacactcc cggtaaaatc ccccggccgc
    16201 gtccctgccg ggccggggat ttttgcttgt tggggaagtc cccaaccccc tattttttga
    16261 aacagaacag agaaaggagt gaccgcgtga acgaagtatt ttctatccaa gtccataacc
    16321 ggcagttttt ggaacagggc aaacaaggcg tctggctgga tttgcccacc acaaccgaaa
    16381 cgctgcaagc cgccctgcgg gatgttggca ttactgccga caatccgcag gattttttta
    16441 tcaacgggta ttcctgcccg gaggacagac accttgccat tccctatgac atggtgctgg
    16501 ccgccgacgt ggacgagctg aatttccttg ccgcccggct gggccagctt gacgccgccg
    16561 agatcgcaga gctgaacgcc gccctgcaaa atccccgtgg cggctttgag aatatcggcc
    16621 agatcatcga ctacaccgaa aatgtggatt actatgtgca tcttcccgac gttagggggg
    16681 atggacagtt gggcgactac tacctgaacc gttccggcat ggtggatatg ccggaggaat
    16741 ggaaagccgg gattgatacg gcgcagtttg gacgccacat tgcccagcag gaacaaggcg
    16801 tttttaccca gtacggctat ctggtgagaa gcggcgacga atggcagcgc gtccatgagg
    16861 gccagccggt gccggaggaa taccgggtga tgcactaccc ccagccggaa gctgaccggg
    16921 aggaaccgca ggcccagcag ccggaggcat ccgaaagccc cgacaaagcg ccgcccccgg
    16981 ttatcccgat catgctggac ggcatgaaca gcaaagaacg catgaaagtg attactgacc
    17041 gtctggaaac cggcgtacag gagctttttg aaagtagccg ctatatggct tacttatccg
    17101 ctatgtccaa gatgcacgat tacagtctga acaatacgct gctgatcgcc atgcagggcg
    17161 gttcgattgt ggcgggctat aacaggtggc aggatgaatt tgaccgccat gtgaagcggg
    17221 gcgagaaagg cattaagata ctggccccgg ccccgttcaa ggttaagcag cagatggaga
    17281 aaatcgaccc ggccacccag cagcccgtga tcggcgcgga cggcaagccc gtgaccgaag
    17341 aacaggaagt gacggttccg gcctttaagg tggtgacagt ctttgacatc agccagaccg
    17401 agggcaagga ttttcccatg ccctatatca gtgaacttac cggcgacgtg cagcagtatc
    17461 aggatttctt tgccgcgctg gaacggacat caccctatcc cataagcatt gagccgattt
    17521 cagacggggc caagggccgc tgcttctacg caaaacagcg cattgccatc aacgagggca
    17581 tgagcgaatt gcagacccta aagaccgcca tccatgagat tgcccatgcc aagctccacg
    17641 ccattgaccg ggaggccccc gccgaggaac aggcggcccg gccagaccgc agcacccgcg
    17701 aggtacaggc cgaaagcgtg gcctataccg tctgccagta ttacgggctg gacacgtcgg
    17761 actattcttt tggctatgtg gccgattgga gcagcggcag ggagttatcc gagctgaaag
    17821 cctcccttga aaccatccgg gccacggcca gcgaattgat aaccgagatc gacggccatt
    17881 ttgcccagct tcagcaggag cgcgaggccc ggcaggaaca ggaggccgtc acggttgacg
    17941 ttaccccgga ggcagcggcc ccggcgcagg ccgaggaaga acaggccccg gcccttgatc
    18001 cggcagccga gccgcttgtg actgtgctat ggagcgaaag cccccagcta aaggacggcc
    18061 agcaaatgcc gttgcatgag gccgacgcgc tgtttgcccg tctggatgca gcatatccgg
    18121 cagaggcagg ctatgacaaa accgcgttcc gcattgactg tatctttcag ggcgagccgg
    18181 acagctacga ggggcggcag gattttggag atagggacgg ttccctgatc gaacatatcc
    18241 agagctacca ccaattttac gcgcaggacg aaaactggaa aaagcatgta atcgctacaa
    18301 ccggcataga gggctgggag caggacaagg cccatcggga tatgctgtta ggcgagtttg
    18361 tcccctatat gaagctgcat tgcagccttg cccaaatgga acagacggcg cagggccgct
    18421 tacagtccgg ggaaaccctg acgccggagg aaacggccta tttgggggcg cttctctctt
    18481 atgtgcagga gtgccgcccc atgctcaatc agggccagta tcagttgccg gagccgccga
    18541 aactggctga cttcgaccag agcttgcagg actacaaagc acaggtacag gccgagatcg
    18601 cgcaggaggc cgccgcagcc ggtatgaccg tggaggaata cgcggcggct ggctatgaag
    18661 caccccaaca ggacaccttt tccatctacc agcttcgagg cggggaggaa acccgcgact
    18721 atcggtttga gccatacgag cgtttacagg ccgcagggct ggccgtggac agtgccaact
    18781 atgaccttgt ttatacggca ccgctggata aaagcacgtc tttggaggat atttaccgca
    18841 cattcaacat cgaccacccg gcagatttta ggggacacag cctttccgtt tccgacgtgg
    18901 tggtgctgca ccagaatgga caggacaccg cccactatgt agacagcttc ggctatatgg
    18961 aagtgccgga gtttttgcgg gaaaatcccc tgcgtaccgc cgaactgtct acggagcaga
    19021 acgaaaacat gattgacggc attatcaaca atacgccgcc cgaaccgcag ccggaggaac
    19081 ccgaacagct atcttttgag gatgtggcag ttgcttccgg ccagacacag gaggaacccc
    19141 cggaggctgc gcccccgcaa acggctgtcc gctattatcc catcaatgag ggagccgccc
    19201 gccgcgccaa ggaagccatg agcttttctg actaccggcc cggcagcgcc acggcggaat
    19261 accggcacta tgtcgatcag gccgtggaaa ttgcggagcg ccagaaaaag cgtgtggagc
    19321 cagagtatca cgaaaagata gacagcctcc ttgacaccta cgcccgcaag ctggccgaga
    19381 acatgaataa gggctatgag attaccgccc gcgtcccctc tgtcctgatt gccgggccgt
    19441 ccaatttccc ggtacgggcg aaagaaaagc agaacgccgc cagtgaccgc aacatggagg
    19501 aatttcagta cattcagggc ttgcttgaca aaatccgcag caccggcatg ggtggtatca
    19561 gcgccgacga tccgcaggcc gtttccaagc tggaaaagaa actggaaaag ctggaagcgt
    19621 cgcaggagtt gatgaaggcc gtcaatgcct actaccgcaa gcatgggacg ctggacggat
    19681 gcccccatct gaccgaaaga ggcattgaaa acctgaaagc ggatatggcg tcgggctggc
    19741 actatgagaa aaaaccgttc cagtcctggc agctctccaa caataacgcc gagatacgcc
    19801 ggttaaaggg ccgcattgag gaattgaccc gccaaaagga ggcggcctat gtgggatggg
    19861 agtttgacgg cggcactgtg gagatcaacc gggtggccaa ccgcctgcaa atcttctttg
    19921 aggggaagcc ggatgctgct gtacgcgacg agctgaaaag caacggtttc cgctggtcgc
    19981 ccaaggccgg ggcatggcag cggcagttaa acgacaccac aatccgcgtc gctgaccgta
    20041 tcaagtgcat ccagcccctt tccggcgaaa agcccagcgc cttacagctt gcggcccgca
    20101 gggagaaaga aaagccctct atccgggcgc agctcaacgc ggccaagacc gaacaggcca
    20161 agcagccacc ggcgcgggaa aaatcaaagg gattggaggt agagtaaatg cagaagtttg
    20221 aaaatgtgga cgttctggcc gcgctggaac agatcatgcg gcagaacacc gcgttttatc
    20281 aaagtgactt tggcattgac aaagacatta tcaggcggga tgccgccagc gatcaggccg
    20341 cagacaagac cctgctctgg atgtcccggc cctccgggac gtactgtttc cgggaacgtg
    20401 atgttttcct gaaagacacc cgacagtaca acacatggaa attttacggg gagcagaccc
    20461 gtgacaagat actggcctac gcggtggagc tgaccggcac acagggcggc actatccggg
    20521 gcaatctcta tgagctggac tatccgcagc atttccggca cgtcattgaa accgcccagc
    20581 cggtgagtgt gaaccggctc ttttatgagc atggcacccg tgacatcccg gagagccagc
    20641 gttttgacgg aagccctgac cgggtattgg gaaacttttt gtactatgag gcccagcccc
    20701 atgacccggc tgtgctgcag gaggcgctac ggacggagca gcacagccgg gggcgggccg
    20761 tccccggcga tttcaaagtc cacgtcaccg ctttgcatga cagccggatt gaaaccgagg
    20821 cccggcgtat tgtggacagc ctgaaagatc tgtccgcgcc taacagcccc aataaaacgc
    20881 atttcatggt ggagatctcc ccgcactttg cggcgattgc ttccaccaag gataatgacc
    20941 ggctctttgc catgctgccc tataaatccc tgtgttttac cgggatgaaa ggcaggcatg
    21001 gcgtgtatgc cgtgattaac cgggacgagc gccgggatgt gagtattcgc aggccccggc
    21061 cctctatccg caagcagcta tccgacagca aacaggccgc cagcccaaaa aaggcggcgg
    21121 ccagaaccaa aaagaatgaa ttggaggtat aaggaatgat gcactttacc gtggaggaag
    21181 aaaacctgat ctgtatgtac cacaatgcag accggcgcag aacgataaac aggctcaccg
    21241 ccgtcatgcc ggttatggat ggggatatgc gggcgcaggc ccagcagacc ctttccaagc
    21301 tggaacgcat gacggatgcc gactatgacg gccagaagtt ccacttcaca gacgaataac
    21361 gaacagcaga cgccgggcgc ggcggccatg tggtcgcttg cgtccggcgt cttttctttt
    21421 tgcctattcc tacgcattta gaacccggta ttgtcgggga taggatgaag tattcgcttg
    21481 cccctgcaaa catgcgggag aagccttgca gcgggcggtt ttcaatccct taaatgcgta
    21541 ggttgcagct ataattagac ccctgaaaca gatatagccg cgcagcgggt gtatctgttt
    21601 caggggtgtg caggtgatag ccgcaaagcg gcgtaagagg gtgcagccct cttgccgtct
    21661 atctctgttc cagcgggaag ctcggcaatg cttccagcag tttcagggaa atgcgctgcc
    21721 gtatatcttc gtcaatgact ttcttctttt tgccgtttgc ctgtctgatc tccaccgtgg
    21781 aaagctcgtt gatgtagtca gcataatggg ccaacacttt ttccgtggcc cacctctcac
    21841 cggcaacggc ggctttgatc gtttcatagt ccagcagctt ttcttgtccg tccatcctga
    21901 tacccccgtc agactgattt tttctatgat accatattcg gaagcatgga accagagaaa
    21961 acggcgcttt tctgctggta aattctacgc atttagaaca gcacttcggc ggggatagga
    22021 taaaacaccc acataccccg ctgaccatgc cggaaaagcc ttgaaatata agggctttta
    22081 cagctctaag tgcgtaggtt tccagtatct tttccgctgg cgctcggcct ctttgcggcg
    22141 gcgcatccgt ttggagcagg ccgcgcagta tttggcccgg ttagacttcg gcgtgaacgt
    22201 ggccccgcac tcacagcagc gtttcacttc gtcccggtct ttggtgatct cggcatacag
    22261 ggccgcatcc agcgggagga cagcggcctt aaaccagcgg cacagaagcg aataggaaat
    22321 gctctgtacg caaatacatt cttcgccgtc gtccagcaac aggcagttgc cgtcacaata
    22381 gttgcagcac tcatgcgtca gcttccgggc cttgcgatat tgagggtaat tcattcgtgg
    22441 aatactcata gctcttgcgt ccattccttt cccggcgtca tacgcaaaat gccgtccaca
    22501 ttctgcttga tgatgccgta ttcctgcatc tgctttttcg cttcgccgta cttctcatac
    22561 agccgttcct tttcctcggc caatcgcttg tactcggctt tcagggcgta cagatcgggg
    22621 agctttttca caccctgtcc ctttagggcc tttgccgcag cttcatacaa aatcaacgtg
    22681 ctttcatgct cacgccggaa ttttgcctta tccgctgcat tgcggtattc catagccacg
    22741 ggccagagct gcttgtaggt agaaatattc tttatcagca ccgccatatc cgcaagccgg
    22801 tgttccatag ctttgagggt agcggcggcc tcgtcgctgg cggcggtgat ctcggccact
    22861 ttgctttcca aatccgggta ggtgtctatc ccatgttcag tcaggaaatt caaagtcttt
    22921 gcggcctgct tcaaattgtg gagcttcgcc cacctctcat agcctgccga ctgctgggcc
    22981 ttaatgttgt tttccagatc aatccgcaaa gaaatcccct tgcgctcttt gggcgctctg
    23041 gtgataacgc ggcctttgat acgctctttg atggcttcct cggtgtaggc ttcccccaat
    23101 gtcttgcagc gggtaaagcg ttcctgtccg ggagcgcgga aagaaataaa ctttcccggc
    23161 ttgatctcat agccctgcgc ctgcatgagc cgcaggaaat catcaaaatc ttttgcctgc
    23221 gggatagcgg cgtcaatggc tgctttgagc ttcgctttcc agctcgtccc tttccgctgg
    23281 gcgtcccact cggcatagct cttgccctta ctctggccgg gcttcaccac ggacaatccg
    23341 ttttccttgc acagcctgtc gctggtgcgc cggatatagg catagctctg tttgttggaa
    23401 atgtacttgc gctgatttac catatccacg gcacagaaaa taatgtgatt gtggacgtgg
    23461 cccttatcaa tatgggtagt cagcacatag ggatatttcc cttgcagcac ttcctcggca
    23521 agctgcttcc cgatctcatg ggcctgttcc ggcgtggctt cgccgggggc aaaggattgt
    23581 atcaggtggt ggcccagatt attgcccttt tggtatgcct gcgccagcag catttcaaat
    23641 tcaatgtccg ctgtttcata agagcaggca aaggaggcca ccagcagctt gccgtcagtt
    23701 ttgtccgggt tctcgatgta gtcaagggct tttttcagcg ttgacttgat aggatgtatc
    23761 tttgtaactg ccatatctgc gccagcgccc cctttatatc ctcgatgtcc tgtgcgtaca
    23821 ccggccccat accgttcagc ctctttgcaa tctgattgac gttgacgccg atcttctgta
    23881 tctcggcggt ctgcgcccgg atgtcgctgg tgtccatgtt gacgatatag ccgtcaatga
    23941 gcatcttccg ggcgtaggct gaaaagttct tcgtatgcag cagcgccatt ttctgttcaa
    24001 tcatagcccg ttcctccggc gtcacaggga cgcgcagcac gatttttcgc tttctgtttg
    24061 ccatgcttca ccgtcctttc cattcagggg tttggggatt tccccaacaa gcaaaacgga
    24121 cgcggcccgg cagggacggg gcaggccgtt tttccgggag tggctacact ccctatgctt
    24181 gctacgttac tgtttctcca ctaacaagac gtttccaaag ccccaaacta cccgcaaaat
    24241 agccctctgg aaagaaaaaa gtgcaaaaaa atagaagcta cgctgtgcag ctcccgctaa
    24301 aagtaaaaca gaggatgcca gttacacatc ctccgtttca ttcttcttcg gcttttcaat
    24361 tagtatggcg ctgtcaatcg cgccctcgat gatcggcagg tattcttcgg ggcagagttt
    24421 cagcttgtgg ctgacccttt gccgcagttc gctttcctcc cgcatgatct ccgggttaaa
    24481 gtagcgttcc acaggaagcc cgcaggcttt aataagctgt atgacaaccg gcagactggg
    24541 aatggccccg tcgttttcga tgttggcaag atagcgccag tcaatattta ttttctctgc
    24601 cattgctttg cgcgaaacgc gctttgcttg ccgggccgct ttcacatccg cgccgaaagt
    24661 ttcaaagccg ggacaatctt caatattcgc catatcgcac ctcccactta cattgtatat
    24721 ttcatactct gcccgtggaa tgttggtata tgcacctttc ttaaagttta tatttcacat
    24781 tttgtttctt ttattcttcg agcaaaagaa ttataatgta ccctgtggag gtgtactatg
    24841 gactacatga cgttgaaaga ggcaggcgag aaatggggtg tgcccccacg atggataaac
    24901 tactattgtg ctgcgggacg catccccggc gctgtaaaga tggcgacgat ctggctcatt
    24961 cccaaaactg cggaaaagcc gattgacagg cgtacaaaga aaggaaaggc ggttactgaa
    25021 tagtataatg aaaccggcgc attttggata tggttttgaa gagagagaaa gcatggtatt
    25081 ttgtcacgga aaaactataa gatgatagat ggcaggctgc tccagaccga taaaaagtat
    25141 tctcatctga aacagaaaga aaagatttac acatggatgt atgaagaagc gaaactttat
    25201 catgacagaa atggaaaatg cccgaaacaa agacgaggaa gctgctgctg tatcagccgt
    25261 atatggtaga attgaaaagg ccggcatctg gattctttat ggtgaagtct ccagacattg
    25321 gataactgct ggtctaatgc gcctttgagt atggggtgat tgattttaat accggggagg
    25381 tacggctttt tgaggaaaat attctgggat ttacatttgg cgattattac atttcctgta
    25441 actgcaagga cacggatgat gaagatatga ggaatataga cttgctgatt ttctactgtc
    25501 ttgaacggta ttaggactga atctgctgcg cggagtgcgg caagagcgac cggagggagt
    25561 acacacaggc cctgcctgtg tgaaaagcca taccgcaagg tgcattcgca aaaaagaggc
    25621 cgcgcatttt gctgaggctt ggggtgtccc caacaagcaa aacgagaacg ccgcaggcgg
    25681 gcaagtggtg gatcagaata tccgtaaagg atattctgat ccattgcttg ccagtattat
    25741 cgggatgttt tgacaaccgc tgtaatcgaa acatcccaaa cattaagaag gtgttaatta
    25801 ttattatctt gatttaattc gtttaaacca ataatataga tacatacacc atgaaaagaa
    25861 gcgagtatcc caatgacgga tgattaaaat ccgaaaagaa atcgctttga aactacaggg
    25921 aaactacaga ctgttatgtt atcttcttaa aatggaggga tttttatgtc gatacgaatt
    25981 ctacttgtcg aggatgatga tcatatctgt aatacagtaa gggcgtttct ggctgaggca
    26041 ggatatcagg tggatgcctg cacagatgga aatgaggcat acaccaagtt ttacgaaaac
    26101 acttatcaac tggttattct tgatattatg ctgcccggta tgaacgggca tgaacttttg
    26161 cgtgaatttc gtgcgaaaaa tgatactccc attctgatga tgacagccct gtcggatgac
    26221 gaaaaccaaa tccgggcgtt tgatgcagag gcagacgact atgtaacaaa gccattcaag
    26281 atgcagattt tactaaagcg ggtggaagcc ctgctgcggc gcagcggtgc gctggcaaag
    26341 gaaatccgtg tcggcaggct gacacttctg ccggaggatt tcacggtact ttgtgacggt
    26401 acagagctgc ccctgacacg aaaagaattt gaaatccttt tgctgctggt gcagaacaaa
    26461 ggcagaacct taacgcatga aatcattttg tcacgtatat ggggatatga ctttgagagt
    26521 gatggcagca cagtccacac tcatatcaaa aatttgcggg ccaagctgcc ggaaaatatc
    26581 atcaaaacca ttcgcggtgt aggataccga ttggaggaat cataataatg gaaagaaaag
    26641 ggattttcat taaggttttt tcctatacga tcattgtcct gctactgctt gtcggtgtaa
    26701 cagcaacact gtttgcacag caatttgtgt cttatttcag agtgatggaa ttacagcaaa
    26761 cagtaaaatc ctatcagcca ttggtggaac tgattcagaa tagcgatagg cttgatattc
    26821 aagaggtggc agggctgttt cactacaata accaatcctt tgagttttat attgaagata
    26881 aagagggaag cgtactctat gccacaccaa atgccaatac atcaaatagt tttagacccg
    26941 actttcttta tgtggtacat agagatgata atatttcgat tgttgctcaa agcaaggcag
    27001 gtgtgggatt gctttatcaa gggctgacaa ttcggggaat tgttatgatt gcgataatgg
    27061 ttgtattcag ccttttatgc gcgtatatct ttgcgcggca aatgacaacg ccgatcaaag
    27121 ccttagcgga cagtgcgaat aaaatggcaa acctgaaaga tgtaccgccg ccgctggagc
    27181 gaaaggatga gcttggcgca ctggctcatg atatgcattc catgtatgtc aggctgaaag
    27241 aaaccatcgc aaggctggag gatgaaatcg caagggaaca tgagttggag gaaacacagc
    27301 gatatttctt tgcggcagct tctcatgagc taaaaacgcc catcgcggct acaagcgttc
    27361 tgttggaggg aatgcttgaa aatatcggtg actacaaaga tcattctaag tatctgcgcg
    27421 aatgcatcaa aatgatggat aggcagggca aaatcatttc cgaaatactg gagcttgtca
    27481 gcctgaatga tgggagaatc gtacccatag ctgaaccgtt ggacataggg cgcacggttg
    27541 ccgagttgct gcccgatttt caaaccttgg cagaggcaaa caaccagcgg ttcgtcacag
    27601 atattccagc cgggcaaatt gtcctgtccg atccgaggct gctccaaaag gcactatcca
    27661 atgtcatatt gaatgcagtt cagaacacgc cgcagggagg cgaggtacgg atatggagtg
    27721 agcctggtgc tgaaaaatgc cgcctttttg ttttgaacat gggcgttcac attgatgata
    27781 ctgcgcttcc aaggctgttc accccattct atcgcattga tcaggcgcga agcagaaaaa
    27841 gtgggcgaaa cggtttagga cttgccatcg tacaaaaaac gctggatgcc atgagcctcc
    27901 agtatgcgct ggaaaacacc tcggatggcg ttttgttctg gctggattta ccgctcacat
    27961 caacattgta aatatttaaa acttaaatga ttttgaccga caggtataac cctgccggtc
    28021 tttttttgct gctacaggaa aactacagac tggctacagg gaaagtacag atgcgcttgc
    28081 cataataaca atcgtaccag ccacaaatcg tagttttatt gcaaaggagg cattcgatca
    28141 aatggaaaaa agcaactatc attccaatgc ggatcatcac aaacggcata tgaaacaatc
    28201 cgtggaaaag cgggcttttc tatgtgcgtt cattatttcg ttcacagtct gcacgctgtt
    28261 tttgggggtg gagactggct tccgtattgg aggcaacaca gataccgccc atccctgcaa
    28321 ctcatacagg cagcagcact gacgtagtgg agaatttgga ggaaaacgct cttgccaccg
    28381 ccaaagaaca gggagatgaa caggaatgga gcctgatttt agtgaacagg cagaacccca
    28441 tccccgcaca gtacgatgtg gagcttgagc aactatcaaa tggtgagcgg atagatattc
    28501 ggatttctcc ctatcttcaa gatttgtttg atgccgcaag aactgatgga gtttacccga
    28561 ttgtcgcatc cggataccga acaacagaaa aacagcaaga aattatggat gaaaaaattg
    28621 ccgaatataa ggcgaaaggc tacacctctg cacaggctaa agcggaagca gaaacttggg
    28681 tggccgtgcc gggaacgagc gagcatcagc ttggtcttgc tgtggatatc aatgcggacg
    28741 gaattcattc aacaggcaac gaggtttata gatggctgga tgaaaacagc tatcgttttg
    28801 gttttattcg tcgctaccca ccagacaaga cagagataac cggtgtgagc aacgagccgt
    28861 ggcattaccg atatgttggc atcgaagctg ccacagagat gtacaaccaa ggggtttgcc
    28921 ttgaggaata tttaaaacca gaaaaatgag aaaaggatgt tatgttatgg acagaaaaag
    28981 attgacacag cgtttcccgt tcctgcttcc aatgagacgg gcgcagagaa aaatgtgctt
    29041 ttatgcggga atgagatttg acggctgtcg ctatgcgcag acgatagggg aaaaatcgct
    29101 ttcccatttg ctctttgaaa cggattgcgc attatacaac cacaataccg gatttgacat
    29161 gatataccag gaaaacaagg tgttcaactt aaagctggca gcaaagacct taaacggctt
    29221 attgataaga ccgggggaaa ccttttcttt ctggtggctg gtacgccatg cggacaaaga
    29281 taccccctat aaagacggcc ttacggtgac caatggtaaa ctcaccacca tgtcgggcgg
    29341 cggtatgtgc cagatgagca atttactatt ttggatgttc ctgcatacgc cattgacaat
    29401 tatccagcgc agaggtcacg aagtaaagga gtttccagag ccaaacagtg acgagattaa
    29461 aggggtggat gcaaccattt cagagggctg gattgattta aaagtgcgaa acgataccga
    29521 ctgcacctac caaatatgag tgaccttaga tgatgagaaa atcatcggtc aggtgtccgc
    29581 cgacaaagag ccccaagcat tatacaaaat tacaaatggc agtatccagt atgtccgtga
    29641 aagtggcggg atttatgaat atgcccaggt taaacggatg caagttgcct taggtaccgg
    29701 ggaaataata gattgcaagc tgctttatac aaacaaatgc aaaatctgtt atcccctccc
    29761 ggaaagtgtg gatattcagt ctaatggtga taagtcaaga tgaaatttaa gattttc
//
LOCUS       DACCKU010000271        20314 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.054, whole genome shotgun sequence.
ACCESSION   DACCKU010000271 DACCKU010000000
VERSION     DACCKU010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 20314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.054"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            <1..401
                     /locus_tag="IVN13_003113"
     CDS             <1..401
                     /locus_tag="IVN13_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP7013512.1"
                     /translation="DLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVI
                     EGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYHGSAKSNIGIDNLIEVITNK
                     FYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            576..872
                     /locus_tag="IVN13_003114"
     CDS             576..872
                     /locus_tag="IVN13_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP7013513.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            879..2831
                     /locus_tag="IVN13_003115"
     CDS             879..2831
                     /locus_tag="IVN13_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP7013514.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2903..3400)
                     /gene="dfrG"
                     /locus_tag="IVN13_003116"
     CDS             complement(2903..3400)
                     /gene="dfrG"
                     /locus_tag="IVN13_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP7013515.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3762..4802
                     /locus_tag="IVN13_003117"
     CDS             3762..4802
                     /locus_tag="IVN13_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP7013516.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            4900..5088
                     /locus_tag="IVN13_003118"
     CDS             4900..5088
                     /locus_tag="IVN13_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP7013517.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5146..5499)
                     /locus_tag="IVN13_003119"
     CDS             complement(5146..5499)
                     /locus_tag="IVN13_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7013518.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            6030..6452
                     /locus_tag="IVN13_003120"
     CDS             6030..6452
                     /locus_tag="IVN13_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP7013519.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6449..6679
                     /locus_tag="IVN13_003121"
     CDS             6449..6679
                     /locus_tag="IVN13_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP7013520.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7177..7377
                     /locus_tag="IVN13_003122"
     CDS             7177..7377
                     /locus_tag="IVN13_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP7013521.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7404..8597
                     /locus_tag="IVN13_003123"
     CDS             7404..8597
                     /locus_tag="IVN13_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP7013522.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(8665..10230)
                     /gene="guaA"
                     /locus_tag="IVN13_003124"
     CDS             complement(8665..10230)
                     /gene="guaA"
                     /locus_tag="IVN13_003124"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP7013523.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10289..11212)
                     /locus_tag="IVN13_003125"
     CDS             complement(10289..11212)
                     /locus_tag="IVN13_003125"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP7013524.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11408..12013
                     /locus_tag="IVN13_003126"
     CDS             11408..12013
                     /locus_tag="IVN13_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7013525.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12321..13337
                     /locus_tag="IVN13_003127"
     CDS             12321..13337
                     /locus_tag="IVN13_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP7013526.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13478..14779
                     /locus_tag="IVN13_003128"
     CDS             13478..14779
                     /locus_tag="IVN13_003128"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP7013527.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            14825..15487
                     /gene="deoC"
                     /locus_tag="IVN13_003129"
     CDS             14825..15487
                     /gene="deoC"
                     /locus_tag="IVN13_003129"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP7013528.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15508..15906
                     /locus_tag="IVN13_003130"
     CDS             15508..15906
                     /locus_tag="IVN13_003130"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP7013529.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            15972..17054
                     /locus_tag="IVN13_003131"
     CDS             15972..17054
                     /locus_tag="IVN13_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP7013530.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17266..18828
                     /locus_tag="IVN13_003132"
     CDS             17266..18828
                     /locus_tag="IVN13_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP7013531.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            18821..19954
                     /locus_tag="IVN13_003133"
     CDS             18821..19954
                     /locus_tag="IVN13_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7013532.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            19954..>20314
                     /locus_tag="IVN13_003134"
     CDS             19954..>20314
                     /locus_tag="IVN13_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP7013533.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWY"
BASE COUNT         6685 a         3586 c         4156 g         5887 t
ORIGIN      
        1 ttgatttatc aacggtttat caggatatta aagagaaact ttctgccgaa attgtaatca
       61 aacagaaggt agaactgtat cctaatatgt gtgtgacgaa ctttaccgaa tctgaacaat
      121 gggatacggt aatagaggga aacgatgacc ttttagagaa atatatgtcc ggtaaatcat
      181 tagaagcatt ggaactcgaa caagaggaaa gcataagatt tcagaattgt tctctgttcc
      241 ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata gaagttatta
      301 ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga aatgttttca
      361 aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag ttttggagta
      421 caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt
      481 ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta
      541 tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt
      601 tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata
      661 agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat
      721 acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt
      781 tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg
      841 aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag
      901 ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag
      961 atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa
     1021 tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt
     1081 actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa
     1141 aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta
     1201 tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt
     1261 tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag
     1321 atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat
     1381 tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc
     1441 aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt
     1501 atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt
     1561 cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg
     1621 agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa
     1681 caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg
     1741 tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa
     1801 cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct
     1861 atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc
     1921 atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac
     1981 catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta
     2041 ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact
     2101 attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact
     2161 tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac
     2221 atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg
     2281 gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc
     2341 tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag
     2401 aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc
     2461 cattagccac taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc
     2521 tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct
     2581 ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg
     2641 gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta
     2701 caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt
     2761 tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt
     2821 atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt
     2881 tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat
     2941 agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt
     3001 cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga
     3061 tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa
     3121 aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac
     3181 aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg
     3241 ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat
     3301 ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca
     3361 ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa
     3421 ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt
     3481 tagttgaata agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat
     3541 tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat
     3601 gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa
     3661 aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat ttacgagatt
     3721 cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact tcaataaatg
     3781 gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg caaaatgagt
     3841 ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga aaaaagattg
     3901 aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa cagagagaaa
     3961 tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga tattacgtgg
     4021 attctacgac acatgaaatt atactttctt tcttagggaa agtacaaatg gaagtgatta
     4081 gtgcactgtt gcaagaaaag tatcatgtgg agatagaact aaaagagcct acagtcattt
     4141 atatggagag accgttaaaa aatgcagaat ataccattca catcgaagtg ccgccaaatc
     4201 ctttctgggc ttccattggt ttatctgtat caccgcttcc gttgggaagt ggaatgcagt
     4261 atgagagctc ggtttctctt ggatacttaa atcaatcatt tcaaaatgca gttatggaag
     4321 ggatacgcta tggttgcgaa caaggattat atggttggaa tgtgacggat tgtaaaatct
     4381 gttttaagta cggtttatac tatagccctg ttagtactcc agcagatttt cggatgctta
     4441 ctcctattgt actggagcaa gcctttagaa aagctggaac agaattgtta gagccatatc
     4501 ttagttttaa agtttatgca ccacaggaat atctttcacg ggcatataac gatgctccca
     4561 aatattgtgc aaatatcgta aatactcaac tgaaaaataa tgaggtcatt attattggag
     4621 aaattcctgc tcgatgtatt caagattatc gcaatgattt aacttttttt acaaatgggc
     4681 ttagtgtttg tttagcagag ctaaaaggat atcaggttac cactggcgaa cctgtttgcc
     4741 agacccgtcg tctaaatagt cggatagata aagtaagata tatgttcaat aaaataactt
     4801 agtgcgtttt atgttgttat ataaatatgg tttcttatta aataagatga aatattcttt
     4861 aatatagatt tgaattaaag tggaaaggag gagattgtta ttataaacta caagtggata
     4921 ttgtgtccta tttgtggaaa taaaacaaga ctacgaatac gagtggatac tatacttaaa
     4981 aatttccctt tatactgccc caaatgtaag aacgaaactt taattaatgt tcaaaaaatg
     5041 aatataataa caatcaaaga gccagacgcc aagacgcaga gccgataatt tgagaaatga
     5101 aactctcatc ttatcggctc tttttgttta tctgaatttt actgactagc cttcaatatt
     5161 tcttgcttca ttgatacctt ttgctagtgc ttccattaag gatagttctt tgtctgtaaa
     5221 gctatccatg tatttttcta cctgtaacct tcgagtgctt ttaatcaaat tatctgtagg
     5281 taagaaaaat tcatcaatag atacatggag taatgataca agatcataca gcacttgtgt
     5341 acttgggtgt tgtcctttgt tttctatatt tgttaaataa cgtgggtcaa tttcaatcat
     5401 tgttcccact tgttcacgag tgagaccccg tttcattcga gcctctttaa tcgctaaacc
     5461 aacggctcta aaatcgtatt tatcttcttt tttacgcata ctaaatcacc tctatacatt
     5521 ttactgttcc tatataacca taaacaggta tagaaaaaca tgttatatag tttatgagtt
     5581 cctatagaac actgtggaat ttattaatgc gatgaatcca atttcaaata gaaaggtcgc
     5641 tgattttatt tacctataca actttataaa atttagtcat actcctagaa agtagataaa
     5701 attggcgact taatggaggg aaaagtcgcc atgagaaaga taatctgtca tctagttctc
     5761 ttaattaagt tcttatcaaa aaaacttatc cacctgtact ggatagatat gaccatcaat
     5821 gtggttttgc agattacata gatagaaaaa tcaaagcata tgaacagaga aaacaatctg
     5881 tttatgtgct tttttggtca cttaataaat atcttttagg atttctcttc atttatgcaa
     5941 caaatccccc gttcgtgtcg gggtttagta tgaagggaga taaagaagca catgccactc
     6001 cctttcacga cagaaagggg gtgaaaagca tgaaaccatc ttcttttcaa gaaaccatag
     6061 aaaatcagtt tgactatatc tgcaaacaag ttattgaaga tgaacgaaaa gattacttca
     6121 aacatctaga cagactatca gcaaaagaag tctcgttttc tgatatagat aactaccttg
     6181 tcaactcttt ttctactgtt gatacgtacg tcactgattt ccaactattt aaattatttg
     6241 gattaagaat caatgttgaa agtgatttat taagtgaagc cctgcgtcat ctttcagaga
     6301 aaaaacggaa cattatttta cttcattact ttatggatat gagtgatgtg gagattgcgg
     6361 agttactaaa tttgaatcgt tcgactgttt atcgtcatcg aataagtgga ctagccatga
     6421 ttaaagaatt catgaaggag tgtaaagaat gaaaacagct tatccaaggg ttcccttccc
     6481 cctcattgta aaggcgacag atggcgacgt ggaagcaatt aatcagattg ttaaacatta
     6541 tcgagggtat acctcaaaac gttcattacg tcgcatgaca gacgaatatg gaaattctca
     6601 tatggttatt gatgaaaccc tacgtggcag aatggaaacg aaactcatct ctaagatttt
     6661 atcgttcgaa attaaatagt cccatttttt cgtggaagcg tgtcgaatcg tatccacgct
     6721 tcccgatttg ggaggtttgt tattccatca aagcatattg tgtgttcagt aggttttgat
     6781 aggctaacaa gccaaacgtt ctttgacaac caaataaagg caatcgaata cgtttcggta
     6841 agaaaagagc tgatgaacta acacgccatg acctacaaaa ttagtgagcg attcatgtta
     6901 gtaattcgaa agtaatcttt agcaggattg ctgccatgac tttcttatcg tgataatgat
     6961 actcccgtat agccatagtc cgagcgttag aagcgtcaca ggcaatgggt acggctacat
     7021 gagaaccatg cagaagtgaa attcctgtga gctttgctag agctgttcga ttgctaagat
     7081 attcttctct tgtcagtgta ttattataaa actatttgat ataattatag gtgaatattg
     7141 gagttttcct agcctataat tgggaaagga gtaataatga taacggtcac aaaaaaggat
     7201 ttagttgagc taggatatgg aacttctttt tcagcagaca tcataaaaaa agcaaaagaa
     7261 ttgatgatta caaaagggca tatctactat caatctcgaa agctagatag agtccctcgt
     7321 gaagctgttg aagaaattct aggaattcgg ttttcaaatg aaaacaggtc tgagtagttt
     7381 ttgcacagta aggagtaaaa actatggtta aagacccaat aaaaaaagca aagaatggaa
     7441 cctattattt tcgtgcaaat ctaggttatg attctaaagg gaaaaaaatt caaaaatatc
     7501 gtagtggatt cactactaaa aaagaagcaa gagaagaata ttcgaaactg ctattgatga
     7561 aaccagaaga attaagtgag aataaggata aaatgacgtt taaacattat atctttgaaa
     7621 ttttccttcc atggtataag acacaggtaa agctcagaac atatgaaaat cgcttaccaa
     7681 ctattaagaa gcatttctct tattttgata aaatggctgt ttccgatatt gagcctattg
     7741 atgtgcaaaa ttggcaacta aaattatcaa agaagtgtaa gtcttcttat gtaagagctg
     7801 ttcaaggact attttcagtt gctatggatc gagcaattgt ccttgggata accaccacaa
     7861 atccatctaa gattattggc aatgtgaaaa agcaaaagtc taaaattgaa ttttggacaa
     7921 aagaagaatt tgaaaaagta atctctctaa tttacaaaga agattactat caacactttt
     7981 tatttatctc tctgtggttt ttatttatga ctggaatgcg aattggtgaa gctactgcca
     8041 ttcaatggga agatattgat tttgactcag gagtactaac catcaacaaa acactctatt
     8101 acaagaatca atcgaattat agatttgttg aaccaaaaac aaaggcgagc gttcgtcata
     8161 ttgtattaga taaatgcaca ttaacttatt tatccgaatg gaaggaagta caacaaaatc
     8221 taattcagac agactttgta atgagctata acggtattcc tacccaaaaa catacactat
     8281 catatgctat tgaacgctat gctaagctgg ctggtatcca tcgaatcaga atccatgggt
     8341 tacggcattc tcatgcttcc ttgttaatca gtatgggaga aaatccactt atcattaaag
     8401 atcgtctagg acacgaagat attgagacga ctttaggaac gtatggacat ctttatccta
     8461 acagtaattt cgaggtagct aacaaattaa atggagccat ttcctttaaa gaagcaaaac
     8521 aaaattatga tacttcacca aagaatcaat ttactgtaga ctacttgcga aacaaagaaa
     8581 tgaaaaaagt gcaataaaag tgcagtaaaa agataagaag agccagaaac ccttggtata
     8641 agaggatttc tagctctcct acaactattc ccactccact gttgctggtg gtttagaagt
     8701 gatgtcatac acgatacggt tgacgtgggc tacttcgttt acgatacgta cagagatttt
     8761 ttgcaagact tcccatggga tacgggcaaa gtcagcggtc attccgtcga ttgaagttac
     8821 agcacggata ccgactgtgt agtcgtaagt acgtccgtca cccataacac cgacagagcg
     8881 gatccctgga agaacagtga aatattgcca gatatcacgg tcaagtccag cagctgcgat
     8941 ttcttcacgc aagatagcat cagattcgcg tacgatttct agcttgtctt cagtgatttc
     9001 gccaagaaca cgaataccaa gaccaggacc agggaatggc tgacgccaaa cgattgaatc
     9061 aggcatgcct aactgagtcc ctaattcacg gacttcgtct ttgaataatg tgtttaatgg
     9121 ttcgatcaat tcaaattgca tatcttctgg cagtccacct acattatggt gagatttgat
     9181 cgtctgtgct gtttctgtac cagattcgat aacgtcagta tacaacgttc cttgtgctag
     9241 gaaagagacg ccttcttcac cagcaagttt tgttgcttcg tcatcaaaaa catatacgaa
     9301 ttcattccca atgattttac gtttttgttc tgggtcagat acacctttca acttgtctaa
     9361 gaagcgatct cttgcatcga ctttgatgat gttcaagccg aattttccgc ctagactttc
     9421 cattacttgt tccgcttcgc ctttgcgtag cagaccgtgg tcaacaaaga tagatgtcaa
     9481 ttggtcaccg atcgcttttt gtaaaagaac cccaacgaca ctagaatcaa cgccacctga
     9541 aagaccaagc aagacttttt tgtcaccaac ttgttcacgg attttttcta cttgcatatc
     9601 gataaagtta tccattgtcc agttaccttc acagtgacaa acatcaaagg caaagtggcg
     9661 cagcaattcg attccgtatt cggaatgacg tacttctgca tggaattgga taccataaaa
     9721 attacgtgtt gtgtcttgga tagaagcaat cggacaatct ttgcttgttc cgactgtttc
     9781 aaagccttca ggaactttag ttaccaagtc accatggctc atccatactg tttgttgttt
     9841 tggtgtttct ttaaacaaga cagcttgatc gcttgttact tctaacaagg ctttcccata
     9901 ttcgcggttt ttagcaggct ctacggaacc gccaagtttg tacgtgatca attgcatacc
     9961 atagcaaatc cctaatacag gaatacctaa attgaagatc tcaggatcga tcccaaatgc
    10021 attctcatca tatacactgt ttggtccgcc agaaagaatg attccctttg gattcatcgc
    10081 ttttacttct tctgcagtaa tgcggtggct caataattcg gaaaagactc caagatcacg
    10141 aatacgtctt gtgatcaatt ggttatactg actaccgtag tctaatacga taatcttttc
    10201 aacgttcgtc atatcggcaa cgtttgtcaa atttattacc cctatccctt taaattttat
    10261 ctcaaaatag tcaaactatt ttaaaatgct aatattttcg catgaaaatc gaatcgatga
    10321 tatgattttc agttttatgg agaattatat cggctcttcc tcgtgtaggc aagatatatt
    10381 cctgtaaatt tttcaaattc acatttttcc atacatcttt tgccatttta aaggcttctt
    10441 cacgatctcc gatagcaaaa cgataataat aattctctgg attttggaaa gccgtatcca
    10501 gtaacgcacc aaaacgatcc aaataccatt tttcgatcag cacgggatcg gcatcgacat
    10561 agacagaaaa atcaaaaaag tcgctgacgt agatttgctg attcgcaggt aattgaagcg
    10621 tattgatccc ctcaacgatc aagatatcag gctgctggat caattcgtac ttgtctggaa
    10681 tgatatcata cacatcatgt gagtaaacag gtacttttat ctcttcctga ccagatttta
    10741 cacggttcaa gaaatcgatc agcatctcca tatcataact ttcaggaaat cctttacggt
    10801 ccatgattcc acgctctttt aatatacgat ttggataaag aaatccgtca gtcgtgatca
    10861 gctgaacgtt tcttcgtttg aaggtacgag acaaaatcgt ctgcagcagt ctggctgttg
    10921 tactttttcc aacggctaca cttccggcga tcccgataat aaaaggcggt acttctacgt
    10981 atttatgaag aaataaacct ttactcaaga acaaagattc aaattctttc atataaagat
    11041 aaatcagatg agcaagaggt acatagatat ctcgaacatc ctgcatggaa atgcggtcat
    11101 tcaaactttt gatattatct agttcatctt gtgtcaaagg tgcttgccca ttacgataaa
    11161 attctcgcca ttcttctctt gccacacggt agtagttcat tggatcattc atattagcct
    11221 cctgcgttct cccatcaatg gaactatttt aacacaaaat aaacgcaaac cccaccactt
    11281 aagagagatt gccgatcttt ttgtataatc tttataatag aaagaaacgg agtcttattc
    11341 ttgacttgcg cacggataca taaaaggata gactgttgtc atgaaagaaa tgagaaggat
    11401 ggaagacatg acaaatcatt actattctga acgcccagat acggcgcacg attttgaaca
    11461 atgggctttt gagttaaaag gcagaacctt tcaatttgta acagattctg gtgtattttc
    11521 acgtgaaaca gtggattatg gttcacgtgt cctgattgat gctttcgatt gggaagagtt
    11581 acctgatgaa ggaaagatct tagatgtggg atgcgggtat ggaccgattg ggttagcttt
    11641 ggcatttgcg acacagcggt ttgttgaaat ggtcgacatt aatgaacgag cggtaagtct
    11701 agcgcaagga aacgcaaagc gtaaccaaat cgaacatgtt gatatccatc aatcgaatat
    11761 ctatgaagca gttcatgaag aaacctatgc agctatcgta agcaatccgc cgatccgtgc
    11821 tggaaaaaaa gttgtacatg aaattttgac aggcgcatat cctcggttga agaagggcgg
    11881 tacactgaca attgtgatcc agaagaaaca aggagcgccg agtgcgcaaa agaaaatgga
    11941 agaaacattt ggtaatgcag aaatcgtcac aaaagataaa ggatattata tcatcaaaag
    12001 tgtgaaagaa taactagctg aaaaatagtg gagcgacgaa agagtggaac tgggcgtgca
    12061 tgtacgggta cgtatcagtt ctttttttgt tgagaagttt ttaaaaacaa agaactattt
    12121 tttcttcaaa aagaaatagt aggaacagag tcgttgtttt ttacgataaa ataaataaga
    12181 tacagaactt gtatagaagg ctgccaagtc ggtagataaa ggaggttttt atcaaaaaac
    12241 atactttcct tttttctcta ttcttttctt gggggttttg gtacactgat agggaattta
    12301 gatagaaggg agccaatagg atgaagctga ccatcagaga tatcgcagaa atggcaggcg
    12361 tatcagtaac aactgtctca cagattttga ataacaaagg aagcagattt agtgataaga
    12421 caagaaaaaa agtgttgtcg atcgtgaatg aacatcatta taagcccgat tatttcgctt
    12481 caaatgtgat caatcgacat tcaaagacca tcggaatgat cgtccctgat gtcactgatt
    12541 tcttcttctc caaactgatc gaaggtgtag agagctattt gaatcctttg ggctatgtga
    12601 tcatgttatg caattctcgt catagccaag aaaatgagat caagtattta caagagcttt
    12661 cccaccgctc agtggacgga atcttgctgg ccacaccaaa tattttacct gagaaatatg
    12721 cattagacag cggtttttat caaaaaatgc caatcatttt aatcgatcgc ggattgaatc
    12781 gtcgggataa cggacggctt atcgtaaagg aatatgaggg cgcctatcaa gctgtttcgc
    12841 tgtttattga aaatggccat acgaagatcg gcatgctgaa ggaaaccact ggatactatc
    12901 aattagaaga acgtttcaac ggatatcgtc atgcgctgaa agatcatggc ctgcctttta
    12961 atgggaaatt tgttgaacat ggagagttga ctgtccaagg tggatatact gcatcaaaaa
    13021 aattactcaa acataaagat atcacggcta ttttttgcgg gaatgatgcc atggcgatcg
    13081 gatgctatca ggcgatcgat gaattgggca aaaaaatccc agaagatatc tcagtcatag
    13141 gattcgatgg attaaagctg tcagaataca tgattccgca attgacgaca gtgcagcagc
    13201 caagttttga tattggattt tatgctgcac gtttcctgat cgatacgatc gaatttcctc
    13261 aaagaaaagt tccgaacaaa gtgttcgaaa caaaattaat tattcgtgag agcgtaaaag
    13321 tattgacaaa gggctaactc ggcggtatat tgcttatgta agcatttaca aaggctagtt
    13381 agtgcttttt ttatttttag taaaccagtt tactaaagtg gtttaccttt cctaaaaata
    13441 atgtataata ccgaatgaag acaaggagga tcaggcaatg cgaatggtgg atctgattga
    13501 aaaaaagagg gatggaaacg agctgtcgaa agaggaaatc gagtacatcg tcacgaacta
    13561 tacgaatgga aagattccag attatcaagt cagtgcatta ttgatggcaa ttttttatca
    13621 agacatgaca aatgaagaaa tcacgaactt aacattggcg attgctaatt ctggtgatgt
    13681 catcgatttg agttctcttg aaggaataaa ggtagataag cattcgacag gtggcgttgg
    13741 tgacacgacc acattgattt tagcccccct agtagctagt gtcggagtga cagtggctaa
    13801 aatgtctgga cgcggtctag gttacacagg tggtacacta gacaagctag aagcaattcc
    13861 cggatttcag atcgagcttt cggatgaaga atttgtacgc attgttaatg aaagtaaagt
    13921 agcggtaatt ggtcaatcag gaaacttggc accggcggac aaaaaattgt atgccttgcg
    13981 tgacgtcaca gcgaccgttg attctcttcc gctgattgcc agttcgatta tgagtaaaaa
    14041 aatcgcggca ggagcagatg caatcgtttt ggatgtgacc actggagacg gtgctttcat
    14101 gaagaacatc gaagatgcaa gacgtctggc aaagaccatg acgagcattg ggaaactggc
    14161 taaccgagaa acagtggcgg ttatctcaga tatgtcagaa cctcttggcg aagctatcgg
    14221 aaatagtcta gaagttgtcg aggcaatcga aacattacaa ggaaatggtc cagaagatct
    14281 agtagaaatg tgctacgcct taggaagcca aatggttgtt cttgctggca aagctaagac
    14341 aaccgatgaa gcaagaacgt tgcttcaaga agctctggaa tcaggtaaag cgttagcaaa
    14401 attcaaagaa atgatccaaa atcaaggggg agacccagcg atcgtggaac atccagaacg
    14461 aattttgact gcacgctaca caatggagct tcctgctaaa caaagcggag ttgtgtcaaa
    14521 aatcgtggca aatgaattag gaattgctgc aatgatgcta ggtgctgggc gtaaaaccaa
    14581 agaagaagat attgatcacg ctgttggatt gaaactgcat aaaaaaattg gtgacactgt
    14641 gacaaaaggt gagtcactat tgacgatcta tagtaacgac gaagaaatca cttctgtcat
    14701 tgaattatta tataaaaata ttgaaattgg tgaatcggca atgaagccaa cgttgattca
    14761 tgacatcatc acagaataaa ggaaaaaagc ggactttacc gttttaaaga aaaggggaaa
    14821 agaaatggaa ctaaaccgaa tgatcgacca tactattttg aaagcagacg catctaaaga
    14881 agctgtgatg caaattatcg aagaagcaaa aaaataccat ttttattctg tctgcatcaa
    14941 tccagcatgg gttgcgctag cgaaagaaca attacaaggt gagccagtag ccgtatgtac
    15001 agtaatcggg tttcctctag gagcaaacac acccgagaca aaagcatttg aaacaacaga
    15061 tgccatcaat aacggtgcag atgaagtcga tatggtcatc aatatcggtg ctttgaaatc
    15121 aaaggatgat caacaagtcc aaaaagatat cgaagcagtt gttgaagcag caaaagatcg
    15181 tgcgcttgtc aaagttatta ttgaaacatc gttattagat agagaagaaa tcatccgagc
    15241 ttgcgaaata gcaaaagctg caggcgctga ttttgtcaaa acatcaactg gtttctcaac
    15301 aggcggagca aaggtagagg atgtccgttt gatgcgtgaa actgtaggac cagaaatggg
    15361 tgtcaaagct tccggcggta tccataacga agaagaagca atggcaatga tcgaagctgg
    15421 tgctacaaga atcggaacaa gcgcaggagt agcaatcgta tctggttcaa ctggtgaagg
    15481 ctattaattt tttcaaagga gaagctcatg gcagaactca aacaagaatg gatcaacaca
    15541 gctattgaag cgttagataa agcctatgtt ccttattccc attttccggt aggcgcttgt
    15601 ctcgtaacgg aatcaggcaa gatttatcaa ggcatcaaca ttgagaatgc atcatttgga
    15661 ttaacgaatt gtgccgagcg cacagctttt ttcaaagcag tatctgaagg agaaaaaaca
    15721 tttacgcatc tagtcgtagc ggggcacacc cccgatccta tctctccttg tggtgcatgt
    15781 cgacaagtaa tggcagaatt ctgttcccct gatatgccgg ttacgctagt aggagacaat
    15841 ggtgtgacga aggcaacgac cgttcgtgca ctgctgcctt atgcatttac tgaaaaagat
    15901 ctataactca tgttcattca gtcagctgac tgttgaaaaa taaaacaatt attttaggag
    15961 gctttacaga aatgaaaaaa gcaaaattat ttggtttagg tgctgtcgca ctagcagcag
    16021 ggttattttt aggtgcatgc ggcaacaacg gctctactga ttcatccggt ggcaaaagct
    16081 catcagacac aacaactgca gcattgatca cagacacagg tggtgtagac gaccgttcat
    16141 tcaaccagtc agcttgggaa ggactagaaa aatgggggaa agatcaagga ctttcacgag
    16201 gaaatgatgg cttccaatat ttccaatctt caaatgaatc agattacatc ccaaatattg
    16261 accaagcatt gaatgctgga ttcaaaacga tttttggtat tggttataaa ctaaaaccag
    16321 cgatccaaga acaagcaaca aacaatacag gtacgaactt tgttattatc gatgatgtca
    16381 tcgatggatt agataacgtt gtatctgcaa ctttcaaaga caacgaagct tcttatcttg
    16441 caggtgttgc agcagcttat acgactgaaa caaacgtagt cggctttatc ggcggtgtaa
    16501 aaggtgaagt aatcgatcgt tttgatgctg gattcaaagc cggtgttgat gcaggtgcga
    16561 aagaattagg aaaagaaatc aaagtattga atcaatatgc tggtgacttc tctgctccag
    16621 ataaaggacg ttcaattgct caaggtatgt atgcacaaaa cgcagatatc atcttccatg
    16681 cttcaggcgg tacaggtaac ggtgtattcc aagaagcaaa atcattgaac gaatcaggcg
    16741 acaaaaaagt ttgggttatc ggtgttgacc gcgaccaatc agacgaaggc gaatacacat
    16801 taaacggcga aaagaaaaac ttcacattga catctacact gaaagcagtt ggaacagtag
    16861 tagaagattt ggcacaaaaa tcagcggacg gtaaattccc tggcggcgaa cacactgttt
    16921 atggattgaa agaagacggt gttggtctaa cagaaggaca actttctgac gaagcgaaaa
    16981 aagcagttga tgaagcaaaa gaaaaaatca tttcaggtga cgtaaaagtt ccagaaactc
    17041 ctgaagaaaa ctaaaaccat ttctatagcc ttgttagaca catgtttgac aaggctatgg
    17101 tttgattaga acaagaattg ttcatttatc caagctgcct tgtgacagtt tggataaggg
    17161 aacaactccc tgaagtaatg tttgtttcaa aagaagaacg ctcactaagc gtgaatctta
    17221 gtcaatcgtt gccctagata gaaatcgtta ggatgtgaaa aaaaggtggt tcaagaaaat
    17281 attgtcatag aaatgcagaa tattaccaaa gaatttggaa cattcaaggc aaatgacaac
    17341 atcaatttac aagtaaaggc aggagaaatt catgcgttgc ttggagaaaa cggtgctggc
    17401 aaatctacat tgatgaacgt gctttccgga ttattagagc cgacatcagg gaaaattttg
    17461 atgcgtggga aagaagtaaa gatcacaagt ccgaccaaag ccaaccaatt agggattggg
    17521 atggtccatc agcactttat gcttgttgat gcctttactg taacagaaaa catcgtgttg
    17581 ggaagcgagc ctagtcgcgc agggatgctt gaccataaaa aagcacgaaa agagatccaa
    17641 aaagtttctg aacagtatgg attgtcagtc aacccagatg cttacgttcg tgatatttca
    17701 gttggaatgg aacaacgggt agaaatttta aaaacacttt accgaggagc agatgtactg
    17761 atttttgatg agccgacagc tgtattgacc cctcaagaaa ttgatgaatt aatcgtgatc
    17821 atgaaggaat tagtcaaaga aggcaagtcg atcattttga ttacgcataa gttagatgaa
    17881 atcaaagcag tagctgaccg ctgtacagtt atccgccgtg gaaaaggaat cggtacagtc
    17941 aacgttaaag acgtaacctc acaacaatta gccgatatga tggttggaag aacggtttca
    18001 ttcaaaacga tgaaaaaaga agcgaagcct caagaagtcg ttttgtctat tgaaaatcta
    18061 gtggtgaaag aaaatcgtgg attagaagcc gtgaaaaact tgaacttaga ggttcgtgct
    18121 ggcgaagtac ttggtatcgc tggaatcgat ggaaacgggc agtcggagtt gatccaagct
    18181 ttgactggtt tgcgaaaggc agaaagcgga catatcaagc taaaagggga agacatcact
    18241 aataaaaaac cccgaaaaat cactgaacat ggtgtaggac acgtgccaga agaccgtcat
    18301 aaatacgggt tagtcctaga tatgacatta tctgaaaata tcgcactaca aacctatcat
    18361 caaaaacctt acagtaaaaa cggcatgctg aattattcag taatgaatga acatgccaga
    18421 gaattgatcg aagaatatga tgttcgaaca acgaatgaac ttgttcccgc aaaagcttta
    18481 tcaggcggaa atcagcaaaa agcaattatc gctcgtgaag tcgaccgaga tcctgatctg
    18541 ttgattgttg caaatccaac tcgtggactg gatgtaggtg cgatcgaatt tattcataaa
    18601 cgtctgatcg aacaaaggga caaatacaaa gcagtgttac tgatcagttt cgaattagaa
    18661 gaaattttaa atgtttcgga tcgtattgct gttatccatg aaggagaaat tgtcggtatc
    18721 gttgatccga aagaaacatc tgaaaatgaa ttaggtctat tgatggcggg ctatacctta
    18781 gaagaagcac gaaaagagtt agaaaagggg gctggcgcac atgaatgatc gcagcgaaaa
    18841 attccgtaat ttattagtgc ctgtactttc agttgtttta ggattgatct taggcgcgat
    18901 attgatggct gcttttggtt ttcagcccat ccaaggttat tcttctatgc tagatgcagc
    18961 ccttggttcg caaagaggga taggtgaaac gttaaggcaa gcaacgccgt tgatttttac
    19021 agcattaggt ttttctgtag ctaactcagc tgggtttttc aatattgggc tttctggaca
    19081 agcactttgc gggtggatcg tcagtgtctg ggtggcactt gcgtttccag atctgccaaa
    19141 agttgttttg cttccgttat gcgtgatctt aggtgcctta gctggtgcga tggcagcggc
    19201 tgttccaggt ttacttcgag cattttttgg aacaagtgaa gtgatcgtaa caatcatgat
    19261 gaactatatc ttattatacg taagcacatt tgctttacat gaagtgatgc ctgaaagcta
    19321 tcgctcaagt ttggactctt ccaatatgat cagtcaaaat gcctcactgc ggttaccatg
    19381 gttaacacaa gcttttggcg gctctcgcgt aaactcagga ttatttttag caatcatcgc
    19441 gcttattttc gtttggctgc tgatgaagaa aacgacttta ggatttgaaa tccgttctgt
    19501 tggattgaat ccttttgctt ctgagtatgc aggaatgagt agtaaacgta cgatcgtttt
    19561 gtcgatggtc atttctggcg ctttggcagg gcttggcggt gtggttgaag gattaggaac
    19621 gtatcaaaac ttctttgttc aaacgacttc gctatctatt ggatttgacg gaatggctgt
    19681 ttccttgctt ggttctggat cagcaatcgg tattctttta tcagcgatct tgttcagtat
    19741 tttgaagatc ggtggacttg gcatgcagac aggagcaggt gttccgtttg agatcgtcaa
    19801 tgtatcgatt gctttgatta tcttctttgt cgggatcaac ttcctgattc gtttcgtcat
    19861 ggctaaagtc ttccaaggga aaaaacaaga agagatcgtt gcgacaatag aatcgaaacc
    19921 aacgaatcaa aaccatgaag ggggcgaact ataatgtcga ctatcgaatt aattgccttg
    19981 atccttactt ctacactggt ttattcaaca ccgctaattc taacgtcact aggcggaact
    20041 ttttctgaac gaagcgggat cgtaaacgta gggctagagg gaatcatggt catgggtgct
    20101 tttagttcca tcgttttcaa cttgacgatg agtccatcac ttggttctat gacaccatgg
    20161 atcgctattt tagtgggtgg cgtcgtaggt atcctatttt ctctgattca cgcagtagca
    20221 acagtcagtt tgcgtgctga ccatattatc agcgggacag taattaatct gatggcacct
    20281 gctttaggtg ttttcttgat caaagcatgg tatg
//
LOCUS       DACCKU010000272         6775 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.122, whole genome shotgun sequence.
ACCESSION   DACCKU010000272 DACCKU010000000
VERSION     DACCKU010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.122"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            150..1265
                     /locus_tag="IVN13_003135"
     CDS             150..1265
                     /locus_tag="IVN13_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP7013534.1"
                     /translation="MQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEI
                     TNQLTDGQTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDL
                     YASVMIAQAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAA
                     FRKYPSYKESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNY
                     GSTLNNVIETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEG
                     IAWQAPNSGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRL
                     FNPNEKTGTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            1752..2216
                     /locus_tag="IVN13_003136"
     CDS             1752..2216
                     /locus_tag="IVN13_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP7013535.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2200..2829
                     /locus_tag="IVN13_003137"
     CDS             2200..2829
                     /locus_tag="IVN13_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP7013536.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            3183..4661
                     /gene="msr"
                     /locus_tag="IVN13_003138"
     CDS             3183..4661
                     /gene="msr"
                     /locus_tag="IVN13_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP7013537.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4708..5154
                     /locus_tag="IVN13_003139"
     CDS             4708..5154
                     /locus_tag="IVN13_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP7013538.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5353..5868)
                     /locus_tag="IVN13_003140"
     CDS             complement(5353..5868)
                     /locus_tag="IVN13_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP7013539.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            5985..6775
                     /locus_tag="IVN13_003141"
                     /pseudo
     CDS             5985..6775
                     /locus_tag="IVN13_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         2159 a         1175 c         1408 g         2033 t
ORIGIN      
        1 aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt gccaacacaa
       61 cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt tcttcagaaa
      121 caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca gaaaatacaa
      181 tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa gacagtacat
      241 cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa ttgacagatg
      301 ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac tatactggct
      361 atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat agtattgcct
      421 caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg attgctcaag
      481 ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat tacaatctat
      541 ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg gaatatgtga
      601 atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac aaagaatcat
      661 tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt tactactatt
      721 ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg ctaacaggtc
      781 gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa acctataatc
      841 tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg tataaccgtc
      901 atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg ccaactgtag
      961 gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct gtctaccgtt
     1021 tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag atcaactctt
     1081 taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt tccaagccaa
     1141 tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta
     1201 aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga ttttatggtt
     1261 attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag ccaaacaaaa
     1321 acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag tttttgaaca
     1381 ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt actgtttaag
     1441 catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt tcggaaattt
     1501 ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga taagaatcaa
     1561 taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc tacaaaaaat
     1621 ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct gattctttga
     1681 cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca attattagaa
     1741 aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc aaattagccg
     1801 atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact agatacgaga
     1861 tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc
     1921 agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt
     1981 cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc
     2041 agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg ctatccccgg
     2101 ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg aatacccaac
     2161 tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa aaatgatttt
     2221 tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata caaaatccca
     2281 cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc tgtcaatatg
     2341 caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg
     2401 atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat ttttggcgat
     2461 atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc agggaagatg
     2521 ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa gagtttttca
     2581 agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat gcaattcatg
     2641 ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga agcagatcag
     2701 ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat tttatcaata
     2761 ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa gatcactacg
     2821 ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga atcgtgtgtg
     2881 gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac
     2941 ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt catggatgga
     3001 gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga tttggtgatt
     3061 tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt tgcaaacaga
     3121 tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg agtgtaaaac
     3181 atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc aaccagctag
     3241 aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc ataggagaaa
     3301 atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt cccgatcatg
     3361 ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat ctcgctgaag
     3421 caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa gaaacttcag
     3481 agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt ctttcttctt
     3541 atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa agtattgatc
     3601 gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt catgatcgct
     3661 attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa atccgagagt
     3721 tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag actcagctac
     3781 gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc caagaaaaga
     3841 aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac
     3901 cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc caaaagaatg
     3961 cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg cagcagatga
     4021 aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat cccatcatgg
     4081 gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt gattttcagt
     4141 tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat
     4201 tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta agctttcaag
     4261 tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt tttgtcatga
     4321 gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta gggtttgctc
     4381 aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc cgtctgacga
     4441 ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg actaatttta
     4501 ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg ggaacgattt
     4561 tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt tatgaaataa
     4621 aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac gatgattttc
     4681 gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg aataattttt
     4741 acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt atggctgcat
     4801 caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt ggtggctttc
     4861 attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta
     4921 aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt tttaggaatg
     4981 gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga tcaattattc
     5041 tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc
     5101 atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga atgatggaac
     5161 actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga atgcagttct
     5221 agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct ttttcttttc
     5281 caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt ctagaaaggt
     5341 tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct gacaccattg
     5401 gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc caaattcttg
     5461 tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga ttttgttcag
     5521 ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc
     5581 atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct ctttgatcca
     5641 ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt gagagtgctc
     5701 gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg ttgtatagat
     5761 ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat acccagaacg
     5821 tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa aaatcctacc
     5881 tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca tgtgcatatt
     5941 tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa atattggaga
     6001 atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag
     6061 ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac ccaatcttaa
     6121 aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta tttactagtt
     6181 acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt cggatcggtt
     6241 tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc ttggcggatt
     6301 atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg ggaatgcttt
     6361 atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca cagaaaaagc
     6421 aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc tagtcggtgg
     6481 ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt tgatgttttg
     6541 cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga gaaaagtagg
     6601 cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg gactaatcgc
     6661 attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt tattgattgg
     6721 ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc tggct
//
LOCUS       DACCKU010000273         5951 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.131, whole genome shotgun sequence.
ACCESSION   DACCKU010000273 DACCKU010000000
VERSION     DACCKU010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 5951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.131"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            40..1461
                     /gene="lsa"
                     /locus_tag="IVN13_003142"
                     /pseudo
     CDS             40..1461
                     /gene="lsa"
                     /locus_tag="IVN13_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Lsa family ABC-F type ribosomal protection
                     protein"
     gene            1528..2055
                     /locus_tag="IVN13_003143"
     CDS             1528..2055
                     /locus_tag="IVN13_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP7013540.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            2266..3327
                     /locus_tag="IVN13_003144"
     CDS             2266..3327
                     /locus_tag="IVN13_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP7013541.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            3330..4226
                     /gene="murQ"
                     /locus_tag="IVN13_003145"
     CDS             3330..4226
                     /gene="murQ"
                     /locus_tag="IVN13_003145"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP7013542.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            4240..>5951
                     /locus_tag="IVN13_003146"
     CDS             4240..>5951
                     /locus_tag="IVN13_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP7013543.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIG"
BASE COUNT         1989 a         1051 c         1353 g         1558 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacctt ttgacacaca atggaaacta
       61 ggacttatcg gacgaaacgg tcgaggaaag acaactttac tgaatattct acaaaacaaa
      121 ctaccttatc aagggcaagt aatccatcag caagaatttg cctatttccc gcaacagaca
      181 aaagataaag aacgtttaac ctattacgtg ttaaatgata ttacggattt tgagatatgg
      241 gaaatcgaaa gagagctcca attgatgcaa acagatcctg aaatcttatg gagagaattc
      301 agcacactat cggggggaga gaagacaaaa gtcctactgg cacttttatt tgtggatgac
      361 actcatttcc cgttaatcga tgaaccaacg aatcatttgg atatctctgg tagaaaacaa
      421 gtagcggctt atttgaaaaa gaaaaaacaa ggcttcatcg tggtcagcca tgaccgggga
      481 tttatcgatg aagtagtgga ccatgtttta gcaatcgaaa aaagtcaact ggaactttat
      541 caagggaatt tctctatcta tgaagaacag aaaaaacttc gtgatgaatt tgaaatggct
      601 caaaatgaaa aattgaaaaa agaagtcagt aggctaaaga aaacagcagc tgaaaaagcc
      661 gaatggtctc gttcccgaga aggagataaa acaaagaaac aagtcggatt catcgatact
      721 gaatctagac gagtgaataa aggagcagtg ggtgctgatg ctgcacggac gatgaaacga
      781 tccaaagcaa tcgtgaatcg gatggagacc cagatcagcg agaaagaaaa actattaaaa
      841 gatatcgaat atatcgattc tttgacgatg aatagccaag cgtctcacca taagcgactt
      901 ttaagcgtag aagatcttca attagggtat gaaaatctgt tattcgagcc aattcatttt
      961 acaatcgagc ctcatcagcg ggtggcgatt tcaggtccta acggtgcagg aaagtcatcc
     1021 attatccatt atcttctggg ggcattcaac ggcaaggtta taggagaaaa ataccagcca
     1081 aaacatctga gcattagtta tgcaagccaa aattatgaag acaatcgagg aacgttggcg
     1141 gaatttgcag agaaaaacca agtagactac caagcatttt tgaacaacct ccgaaagctt
     1201 gggatggaaa gagatgtttt tcataacaag atcgagcaga tgagtatggg ccaacggaaa
     1261 aaagtggaat tggctaaatc tttatcacag ccagctgaac tatatacatg ggatgaacca
     1321 ttgaattatt tggatgtctt caatcaagaa caattagaac aactgatctt gaacgtgaaa
     1381 cctgccatgt tactagtgga acatgatcaa accttcctgg ataaagtatc tactgagatt
     1441 atttctcttg agagaatcta acccttgtaa tctcaagaac aacaaggtat ggttaagggg
     1501 aactttgcca acgaaggaga gaatcacatg ctttatgtaa cgagacacgg agaaacaact
     1561 tggaacgcac aaggattagt ttgcggacgg gcagatgtgc cgctgactga aaaaggacag
     1621 atgcaggcac agaaactagc agaaaaggta gtagatttgc ctgttccaat cacgaaaatc
     1681 atccattccc ctcttcaacg agcaagagat acagcacaag cagttgctga tcgtctcagc
     1741 ttgccgctga ccgttgatga acgattggta gaaatggatt ttggggatta tgatggactg
     1801 ccaagcaaag atgagaactt tcaaaaagca cgtttagcgt tcgctgtacg attcccaaat
     1861 ggggagtcag tcttagatgt gtatgcacgg atcgttcctt tattgaaaga atgcatcgaa
     1921 gacgaagaaa atgtctatct gttagtttgc cataatgcct tgataagagt gatcaatgct
     1981 tattttcatc cgatgcctaa tgaggggttc tttacattca tggtagataa tacagaattg
     2041 gtttcttatg agtaattatg tttttgtgtt atttaaataa aatatttcaa ggcactgctt
     2101 ttgcttagca gtgccttttt taggttatga caattctttt gataatataa actcaacctt
     2161 tagaaatgaa atttaattat tttaataaaa gtattgaaat tttgtttctt tagatatata
     2221 ctctagtcat agaaatgaag acggatacag aaaaagaggg ataatatgtt tggcttttca
     2281 atatttatga acgattcttt gtcagaagag aagaaacagt atatccgcca aatggcatcc
     2341 aatggctttg tcggtatttt cacttccatg catataccag aagatgatgt ttcggcatat
     2401 caaaagcgat tgatcgaact aggaagcgaa gcgaaaaaga ataaactaga attgatggtc
     2461 gatatttcag gagaggcatt ggaacgagcg ggtttttcat tagaagatct aacgcctttg
     2521 aaagcaattg gtgtaacagg tttaagaatg gattatcata tttccaatca gcagattgcc
     2581 gactggtcac atcaattgaa aatcagtttg aatgctagca cgatcacgcc taaagatatc
     2641 gatgaattga aagaagcaga agctgatttc tcacagattg aagcttggca taattattat
     2701 ccacgtcccg aaacagggct ggacaaagaa tggtatcaga aaaaaaatca atggctcaaa
     2761 attcaaggtc tccttgtcca aggttttgta ccaggagata cagagttgag aggcccacta
     2821 tatcaaggat tgccaacttt ggaagaacat cgagggatcc atcctcttgc aggcgctcta
     2881 gatctattag caagtaacac ggatattgtt tacatcggag atgcaggtct gtcagaaaac
     2941 gttcaggagc aatttgcttc tttccaaaaa gaacagacag tcttgctcca tacggaacct
     3001 gtagatgaag aattttacga atatatatta gggaagcata cgaatcggca agacgatgca
     3061 agagatgtca tacgaagcgt agatgcacgt tttcgagaga ttccgcctat cccagcaaga
     3121 aatacagcta cgcgtatgaa agggtctatt actttagata acgaaaagta tttgcgctat
     3181 atgggtgaga tccaattaac caaatatgat ttgccagcgg atgaaaaagt gaatgtagta
     3241 gctaaagtta tcaaagaaga gctaccattg atcaaccaaa tcaaagctgg tatgaactat
     3301 caatttataa gaaaagaagg aagataaaaa tgagcaaaat caatttagag aacctaacaa
     3361 cggaacgcag aaaccaagaa acttttggat tagatgagat gagtatttct caagccgttc
     3421 aactaatgaa caaagaagat cagaaagtgg ccgctgcggt tgaaaaagaa ctgacaagta
     3481 tcgaaccagt gattgctaac acgattgagt cctttaaaaa aggtggacgt ctgatttata
     3541 tgggagccgg aacaagtgga agactaggtg tgttagatgc tgctgaatgc gtaccgacat
     3601 tcggtgtaga accggaaatg gtcataggtc taattgctgg tggagaagaa gcaatgactg
     3661 tagctgtcga aggggccgaa gattcttatg agctagggca agaagatcta atcaagctga
     3721 atttgacaaa aaatgatata gttattggta tcgcagctag cggtcgtaca ccatatgtga
     3781 tcggtggatt agattacgca aaatcgatcg gtgctgtcac aggaacgatt tcttgcaata
     3841 aacaagccga aatcagtaaa cacgcggatt tcccaatcga agtcgatgca ggtccagaat
     3901 ttttgacagg ctctactcga ttgaagtcag gaacagcaca aaaacttatc ttgaatatga
     3961 tttctactat cagtatgatt ggtataggta aagtattcaa taacttgatg gtcgatgtca
     4021 aaccaacaaa tgaaaaacta atcgaacgct caaaacgaat catcatgcaa gcgacagaag
     4081 ctgattacga aacagctgaa aagtactttg aagaagcaga tcaaaatgtt aagctggcta
     4141 tcgtcatgat tttgacaaat agtgataaag aggaagctca agcaaaatta gctgaagcaa
     4201 acggatttgt aaaagggacc ttatgagggg gaaaagacca tggcaaaaaa cgaactgact
     4261 ttggaagaac tggcaaaaca aatttataaa ggggcaggcg gcatgggcaa tgtagaaagc
     4321 attgtccact gcatgactcg tgtgcgtatg aagatcatca atgatgatct tgtaaatgtc
     4381 tctgaattaa aagcgattcc aggagtgctt ggtgtagtag aagatgaaca gcttcaagtc
     4441 atcatcggac ctgggaaagt gaacaaagta gcacagcaaa tggttgattt ggcaggcgtg
     4501 aaattaggag aaaaactttc ttctgtaagt acagctacag caaattcatc agcatctgga
     4561 aaagaccgtg taaacgaacg ggcacaagaa atgaaagcgg cacaaaaatc gaaacaaaaa
     4621 ccatctaaaa tcaaatcggt attaaaagac atttctaata tctttgtgcc aatgattcct
     4681 gcatttgtcg gtactgggat cgtggcaggg attgccgcag ttttagcaaa cttagtcaca
     4741 gccggaacac ttgatgcagc aacatggcaa caatatatcg atgtgatgaa catcttgaaa
     4801 aacgggatgt ttagctacct agttatctat acaggtatca atgctgcaca agtttttggt
     4861 gcaacaccaa ctcttggtgg agttatcggg gccgttgtga tgctgacagg gatgaatccg
     4921 gaagcaccac tgaaaaactt atttactggt gaagctcttg cagctggaca aggtgggatc
     4981 ctaggtgtca tcttcgctgt ttggctattg tcaatcgttg agaaaaaact gcataaagta
     5041 gtacctgatg cggtagatat catcgttaca ccaactcttt cattgattgc catcggtctg
     5101 attgaaattt tcttcatcat gcctttagca ggtttcattt ctgatggcat ggtcggtggg
     5161 atcaactggg tacttggcgt tggtggagca tttgccggct tcgttctagg cacattattc
     5221 ttgccgatgg ttatgttcgg actgcatcaa attttgactc caatccatgt gcaaatgatt
     5281 gatgaaactg gtcgtacgct attgcttcca attcttgcga tggctggtgc aggacaagtg
     5341 ggtgcagcat tggcattatg gattcgctgc aaaaaagata aagaattaac agaaatgatc
     5401 aaaggagctt taccagtcgg gatcctagga atcggcgaac cattgattta cggagtaaca
     5461 ttacctctag gacgcccatt tattactgct tgtatcggtg gaggtatcgg cggggcagtg
     5521 atcggaatga tcggaaatgt cggggctatc gctattggac cttcaggagc cgctttgatc
     5581 ccattgatct ctgacggtaa atggttaggc tacatccttg gattattggc agcttatgct
     5641 ggtggcttcg ttgctacatt cttcttcggt attcctaaag accgtttaga aaaagaagaa
     5701 ttagcgactg aaacagccac agtaaatgaa gtgcctgaaa aagtagtacc tcaaacttca
     5761 gctgaaagtg tgactatcct ctcatcagtt gctgacggaa cagttgtttc tttagataaa
     5821 gcaagtgatc cagtttttgc acaaaagatg atgggtgaag gctactttgt agaaccagaa
     5881 aacggacata tctattctcc agttactggg gtagtcagct ctgtcttccc aacgaaacac
     5941 gctattggga t
//
LOCUS       DACCKU010000274         4290 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.152, whole genome shotgun sequence.
ACCESSION   DACCKU010000274 DACCKU010000000
VERSION     DACCKU010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.152"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            243..2129
                     /locus_tag="IVN13_003147"
     CDS             243..2129
                     /locus_tag="IVN13_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP7013544.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            2141..3088
                     /locus_tag="IVN13_003148"
     CDS             2141..3088
                     /locus_tag="IVN13_003148"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP7013545.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            3108..3635
                     /locus_tag="IVN13_003149"
     CDS             3108..3635
                     /locus_tag="IVN13_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP7013546.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(3698..>4290)
                     /locus_tag="IVN13_003150"
     CDS             complement(3698..>4290)
                     /locus_tag="IVN13_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP7013547.1"
                     /translation="HGIGTGLSIAGLVVLLVKGAHLHSSLHIVSYAIYGSMMILLFLT
                     STLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCLLSIRGWLGWTLFVLIWLFAIL
                     GIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPLYHSLGMTGVILMVAGGLSYTI
                     GAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
BASE COUNT         1538 a          754 c          903 g         1095 t
ORIGIN      
        1 atacgatgaa caaaccatct gataactttt ctttccgatt tgcgtttcga gcaaagtcaa
       61 aaaaaacgag tccaataaaa taaaaaggta aaaattttca agttttataa aaaacaaaac
      121 atatcagaac aggatcgaat cggtcctgtt ctttttttga gaacacaaaa agaacaaata
      181 ccaaaaaaat tttcctgaag ttcactattt ttatgataca atatccaaga taaggaggga
      241 acatgttgaa agaattaaaa gtaactgacc tcaaaaaaac ttatggtgaa aaagatttgt
      301 ttgaccagat ttcttttttg gtccacgaaa aagaccgtat cgggttgatt gggacgaacg
      361 ggacgggtaa aacaagtctc ttaaatatta ttgccggtat cgatagcagc gacggtgatc
      421 ggcaaacagt gttttatcca actgattacc gtattggtta tctttctcaa gcagctgaat
      481 tttccgatga attaactgtt ctccaagcag tctttcaagg aaacagcccc ttgatccaaa
      541 ctgttcgagc atatgaggaa gcattgatcg aactagggga aaacggagaa gaccctgatg
      601 tgcaaaaacg ttatgctaaa gcagaagaac aaatgaataa agaagatgct tggactacag
      661 ataccaacgc aaagattatt ttgcaaaaat taggaatcag tgaattagat aaaaaaatca
      721 gtactttatc aggtggtcag aaaaaacgag ttagtctggc gcaagtgttg attgatgaac
      781 ccgatttact attactagac gaaccaacaa accatttaga ttatgaagcg atcgagtggt
      841 tggaaaacta tttgaaacaa taccgtggcg cactattgat ggttacccac gatcgttatt
      901 ttctagatag agtagcaaac cgaatcttcg aactttcatt tggaaaatta tacgagtata
      961 aaggaaatta tgaagcttac gtcttagaaa aagcagaacg tgatcgtgta gaagtagaac
     1021 aagaagaaaa gcgcaagcgg ctttataaac aggaattagc ttggatgagg gctggcgtac
     1081 aggcacgtgg caccaagcag caagcacgga tcaaccgttt cgaagatcta aaggagaact
     1141 tgtaccaagt aaaccaagaa gatgatttag agctgaacct agcgacacaa cgtttaggga
     1201 aaaaagtact ggaaatcaaa gatggcagtt accgtataaa tgaccaaaca ctgctagaac
     1261 atttagattt attgattcaa tctcgggaaa gactaggtat tactggtaaa aatggtgcgg
     1321 gaaaatcgac attgcttaat attctagctg gcagaattcc cctggatagc ggtacaatgt
     1381 ctatcggtga aacggttcat cttgcgtatt atacacaaga aaatgaagaa atggcaccag
     1441 ataaacggat gattgcttac ttacaagaag ctgctgaaga agcaaaaaca gcagatggca
     1501 gtcaaatcgg agtagcagaa ttacttgaac gatttttatt tccaagattt atgcatggga
     1561 cactgatagg caaactttca ggcggagaaa aacgccggtt atttttgttg aaactgctga
     1621 ttcagcaacc taatgtttta ttattggatg aaccgaccaa tgatttggac atagcaacat
     1681 tgacgatttt ggaagattat tttagaagtt ttccaggtgc tgtgatcact gtttcccatg
     1741 accgttattt cttggacaag gtagcagata agttattagt attccaagga aacggaaaac
     1801 aagaacttta ttacggaaac atgtccagtt atttactgaa gcaaaaagag acccagcagc
     1861 ctgctgaaaa agcgaaacca aaaatacaga gcaaggaacc tgctgaaaag aagaaattat
     1921 cttacatgga acaaaaagaa tgggaaacga ttgaagatga gattgcagaa cttgaagaaa
     1981 aaatctcatt gctccaagaa gagatgaatc atcaaggaga taatttcact cgattacaag
     2041 aattgcaaaa cgacgtatct gaaacagaag cacaacttga agaaaaaatg gcacggtggg
     2101 aatacttgag tgaatgggta gaagattagg aggaaaaagg atggaacaag cgtatcttga
     2161 cttaggaaga aaattgttgg aagaagggca tcgtaaagaa gacagaacag gcacaggcac
     2221 attaagctta ttcggatacc aaatgcggtt tgatctgcaa aaagggtttc ctttgttaac
     2281 aacgaaacga gtaccttttg gattgatcaa aagcgaattg ctttggtttt taaaaggaga
     2341 tacgaatatc cgctatctat tacaaaataa aaaccatatt tgggatgaat gggcatttga
     2401 acgctacatc aaaagtccag actataatgg accagatatg actgattttg gccatagatg
     2461 tttgacagat cctgaattca atgaagaata taaaaagcag agcaagcttt tttgcgagaa
     2521 gatcctaacc gatgattcct ttgctgaaac atacggcgat cttggacaca tctacggcta
     2581 tcaatggcgt cactgggaaa caaaagacgg tggctttatc gatcaaatca aacaagtgat
     2641 cgaagcaatc aaaaagactc ctgattctag aagattgatc gtttctgcat ggaatccgga
     2701 agatgttcct agcatggctt tgcctccttg tcatacgatg tttcaatttt atgttcaaga
     2761 gggacggcta agctgccaat tgtatcaacg cagtggggat gtgtttctag gcgttccgtt
     2821 caacattgca agttatgcgt tgctgactca cctaattgca catgaaacag gtttagcggt
     2881 tggcgaattt gttcacacat taggagatgc tcatctttat atcaatcata tcgaacaaat
     2941 gaaagaacaa ttgagccgag aaatacgcgc atttccgact ttagttttga atcaggaaaa
     3001 gcagtcagta tttgattttg agatggaaga tatccaatta gaaggatatg acccacatcc
     3061 ggcgatcaaa gctcctatcg ctgtttagaa tcagaaagga tgagaaaatg ttagctgcaa
     3121 tttgggcaca ggatgaaaac ggattgattg gaaaagaaga ccgtcttcct tggcgtttgc
     3181 caaatgatct gaaattcttc aaacaaatga cagaagcaaa tacactagtg atgggacgaa
     3241 aaacattcga agggatgggt agccgtccgt tgccgaatcg tcagacgatt gttttgacga
     3301 gagattcttc ctataaagca gaaggcgtgc atgtcatgca tagtgtggaa gacgtgcttg
     3361 ctctggaaaa agaaacagac ggtatctttt tcatcgctgg agggtcagct gtttatcaag
     3421 aatttctgcc attttgtacg atattatacc gtacagtcat ccatcatgta ttcgatggag
     3481 atgcttattt tccacctgtt gactggtctg attggtcact gatcaatact agtcaaggag
     3541 aaatggatga aaaaaatcgt tatccgcatc aatttgaaac gtatcagcgt aatgagaatc
     3601 ctgtaaaaag cgggacaggc gaattgacga tataaaaaag ctatggatag actagctgtt
     3661 atttagagct gtcccatcca tagctttatt ttttttatta agtaaagaat aaaacagaga
     3721 agtacataaa acctgcacct aacataacaa acaaatgcca aacaacatgc atatagcgga
     3781 tagaattcaa gctataaaag aatgcgccta tcgtgtaaga aagcccgcct gctaccatca
     3841 aaataacacc agtcattcca agagagtgat aaagaggaac agcagccacg acacagagcc
     3901 accccataaa aatatagatg atcgtagata cttttgatac tctctcccgt ttatgcaacg
     3961 tcaacgcttt atatataatg ccaagaatcg caaacaacca gattaataca aataaagtcc
     4021 aacctagcca tcctcggata ctcagcaaac aaaaaggagt ataacttcct gcaatcaaaa
     4081 gaaaaatcga actatgatcg aatacttgga acaccttttt tgcttttgta aaaatcaatg
     4141 aatgaaacaa agtagacgtt aaaaataaaa gaatcatcat cgatccatag atagcataag
     4201 agacaatatg gagggaggaa tgcaagtggg caccttttac taaaagaacc actagtcccg
     4261 cgatactcaa tccagtccca attccatggg
//
LOCUS       DACCKU010000275         3081 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.166, whole genome shotgun sequence.
ACCESSION   DACCKU010000275 DACCKU010000000
VERSION     DACCKU010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.166"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            40..867
                     /locus_tag="IVN13_003151"
                     /pseudo
     CDS             40..867
                     /locus_tag="IVN13_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294513.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
     gene            921..1724
                     /gene="lnu(B)"
                     /locus_tag="IVN13_003152"
     CDS             921..1724
                     /gene="lnu(B)"
                     /locus_tag="IVN13_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lincosamide nucleotidyltransferase Lnu(B)"
                     /protein_id="HAP7013548.1"
                     /translation="MLKQKELIANVKNLTESDERITACMMYGSFTKGEGDQYSDIEFY
                     IFLKDSITSNFDSSNWLFDVAPYLMLYKNEYGTEVVIFDNLIRGEFHFLSEKDMNIIP
                     SFKDSGYIPDTKAMLIYDETGQLENYLSEISGARPNRLTEENANFLLCNFSNLWLMGI
                     NVLKRGEYARSLELLSQLQKNTLQLIRMAEKNADNWLNMSKNLEKEISLENYKKFAKT
                     TARLDKVELFEAYKNSLLLVMDLQSHLIEQYNLKVTHDILERLLNYISE"
     gene            2412..3081
                     /locus_tag="IVN13_003153"
                     /pseudo
     CDS             2412..3081
                     /locus_tag="IVN13_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT         1074 a          455 c          619 g          933 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaacccc ttaaagaaat aggaaggttg
       61 tcttatgcag caaaacgtag ttcaaactgg tcaaataaag tagaaaaaag taaatatgga
      121 acaacaaatt ctggttcaaa actggataag ggttatgttg gacataaggc tgcaaaagcg
      181 atgaaacgtg ccaaaaatat tgagtcaaga catcaggaag ccgttttaca aaaatcagaa
      241 ctgctccaca acattgaaca atatgatgac ttaaaaattt caccacttga atttcacaaa
      301 gagtgcttaa tagaagcgaa tgatttatca ttgtcttatg gagataaaga agtatgcagt
      361 aatcttaatt tcagagtcaa tattggtgat agagttgcca ttatcggaaa aaatgggagt
      421 ggtaagtcta gtatcctaaa attgattaat ggagatgata ttaaatttac cggaaatttt
      481 atgctagcaa gtggactaaa aatttcttat atttcgcaag atacttcata tttaaaaggt
      541 aatctatctg aatttgccta taataataag atcgatgaaa ctctatttaa aacgattctt
      601 cgtaaactgg attttaatag agagcagttt gataagaaca tggtggattt tagtgctggt
      661 cagaaaaaga aagtactaat tgctaaaagc ctttgtgaaa gtgcacattt gtatatatgg
      721 gatgagccat tgaactatat tgatattttt tcacgtatcc aaattgaaaa aatgattttg
      781 gaatattgtc ctacactatt gtttgtggag catgatgatg ctttttgcaa taacatttgt
      841 acgaaaaata ttaatttagg tttgtagaga ttttgagttg ccacaataac taaaagatat
      901 ttacatgaaa gggtgaagaa atgttaaaac aaaaagaatt aattgcaaac gttaagaatc
      961 ttactgagtc agatgaacga attacagctt gtatgatgta tggatcgttt accaaaggag
     1021 aaggtgacca atactctgat atagagttct atatattttt gaaagatagt ataacctcga
     1081 actttgattc atccaactgg ttgtttgacg tagctccgta cttgatgctt tataaaaatg
     1141 agtacggaac agaggtagtt atttttgata atcttatacg tggggaattt catttccttt
     1201 ctgaaaaaga tatgaacata atcccctcgt ttaaagattc aggttatatt cctgatacga
     1261 aggctatgct tatttacgat gaaacagggc aattagaaaa ttatttatca gagataagtg
     1321 gtgcaagacc aaatagactt actgaagaaa atgctaattt tttgttgtgt aatttctcta
     1381 atctatggtt gatgggaatc aacgttctaa aaagaggaga atatgctcgt tcattagaac
     1441 tcttatcaca acttcaaaaa aatacactac aacttatacg tatggcagaa aaaaatgctg
     1501 ataattggct aaacatgagt aaaaaccttg aaaaagaaat tagccttgaa aattataaaa
     1561 aatttgcaaa gaccactgct cgattagata aggtagaatt atttgaagcc tataaaaatt
     1621 ctttgctatt agttatggat ttgcaaagtc accttattga acaatacaac ttaaaagtta
     1681 cacatgacat tttagaaaga ttgttgaatt acattagtga ataggagagt aacgttattc
     1741 acagtattgg gtactgaaaa ttaacagcat acctcagttt ttcaaaaaac agcgaagcct
     1801 atggatatgt tcccgtctac cgatagtgca aaaagtgtcg tggatatgtt tccgagaacg
     1861 gacatacaca aatgacattc attctgtcct ctcaatccat gtggaagcag tatgtctgtt
     1921 aacaagaaaa taattattgt tcgtcctgac gagttgttag actcgaaaag gataaacatc
     1981 atttttagaa ccggtagccg atttttggct agcggttttt tgggctcttt ggcaaatagt
     2041 ggtgattgaa tctcgcgcta tcctatggaa aaccgccttg tgtgaggggc tgcgagaccg
     2101 cttcgatcct agaattgctg aagcacgaat ctagcgtcac tcacatttca tcttttgacg
     2161 tagctgagac aaggtgtttt tcttcgatcc gctagtggtt aatctctttt tggtgcaaat
     2221 atggtttaga atttctttgc atggcttaag ggagtggaga atcggatatt agtaggattt
     2281 acatccacag ataaaaatag gcttgatttt aacagcgaaa ctaaaatacc ctacttaaat
     2341 gaagtgatag attagatgtt gggttgttag gagatgctta aaaatcctat gcaatcaata
     2401 gtttttagtg tttgttgact tgatccctaa atcgcctgag tactaaacgt tgagtgtgtc
     2461 tgcttactgt cactagccag taagtgagac tgtagttaat ctctcatttt ccccagtatt
     2521 atgttgaaaa tagccatgaa gcgattattt ctaaagagtt atttttgcag gctcaagaag
     2581 aaatttatcg gagaagtaat atctacacag gagaaggcaa gaacaaaaga atttatagta
     2641 gcaagtacgc tttaagtgcc atcaccttct gtggatattg tggcgatatt tataggagaa
     2701 cctattggaa tattcatggc agaaaagaat ttgtctggcg atgcgtgact agaatcgagc
     2761 agggtcccga ggtatgtaag aaccgaaccg taaaagaaga tgaactctat ggtgcggtaa
     2821 tgaccgcaat taataagcta cttgctggtg gcaacaatat gataaagaca ctggaagaaa
     2881 atattcatgc tgtgattgga gaaaccacag aataccaaat ttcagagatt aataccctac
     2941 tagatgaaaa acaaaaagaa ctaatcaagc tggccaataa aggtcaagat tatgaatacc
     3001 tggtagatga gatcgatgag atgcgagata agcgtcagac tcttttagta gaagatgcct
     3061 ccctcagtgg cgagaacgag c
//
LOCUS       DACCKU010000276         3026 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.168, whole genome shotgun sequence.
ACCESSION   DACCKU010000276 DACCKU010000000
VERSION     DACCKU010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.168"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            47..1018
                     /locus_tag="IVN13_003154"
     CDS             47..1018
                     /locus_tag="IVN13_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP7013549.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="IVN13_003155"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="IVN13_003155"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP7013550.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="IVN13_003156"
     CDS             2045..2653
                     /locus_tag="IVN13_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP7013551.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          814 a          635 c          828 g          749 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa accgctgttg ggcagacctt
     2821 tcccacgccc attttgtatt ttgctggcgg cggttttgag aaatcaaggc cgccgttctt
     2881 ttctggcagc aaaaggtgtg gcggcccata ggaggatgcc gctatgctgt tttgctattg
     2941 ccatgctcta atccacttcc accactcaaa aaagccttgc ccctccatga actgaacccc
     3001 aaaagttgaa ctatttaatg gactgt
//
LOCUS       DACCKU010000277         2995 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.169, whole genome shotgun sequence.
ACCESSION   DACCKU010000277 DACCKU010000000
VERSION     DACCKU010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.169"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            3..188
                     /locus_tag="IVN13_003157"
     CDS             3..188
                     /locus_tag="IVN13_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005611413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP7013552.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGDEIKISNE"
     gene            203..1072
                     /locus_tag="IVN13_003158"
     CDS             203..1072
                     /locus_tag="IVN13_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HAP7013553.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARSTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="IVN13_003159"
     CDS             1053..1787
                     /locus_tag="IVN13_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008766909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP7013554.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="IVN13_003160"
     CDS             1820..2728
                     /locus_tag="IVN13_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HAP7013555.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..2973
                     /locus_tag="IVN13_003161"
     CDS             2725..2973
                     /locus_tag="IVN13_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin acetyltransferase"
                     /protein_id="HAP7013556.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLLTRGAS"
BASE COUNT         1047 a          421 c          662 g          865 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttta
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agatgaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcattgt attagggggc tcacgtgcaa gaagcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc ttttaacccg tggtgctagt taattttaat atcctaaaga atatc
//
LOCUS       DACCKU010000278         2040 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.197, whole genome shotgun sequence.
ACCESSION   DACCKU010000278 DACCKU010000000
VERSION     DACCKU010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.197"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            complement(<1..874)
                     /locus_tag="IVN13_003162"
     CDS             complement(<1..874)
                     /locus_tag="IVN13_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP7013557.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLF"
     gene            1427..1975
                     /gene="aac(6')"
                     /locus_tag="IVN13_003163"
     CDS             1427..1975
                     /gene="aac(6')"
                     /locus_tag="IVN13_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7013558.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          666 a          366 c          399 g          609 t
ORIGIN      
        1 caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg
       61 ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg
      121 ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga
      181 tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg
      241 cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc
      301 tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata
      361 aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat
      421 caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt
      481 cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg
      541 tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac
      601 caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa
      661 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
      721 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
      781 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
      841 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
      901 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
      961 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
     1021 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
     1081 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
     1141 gattattata tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa
     1201 agataatcta tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga
     1261 tctttcctat tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa
     1321 taatgaaaga atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt
     1381 ttttcaaaag gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga
     1441 atttgaccgt aataatccag tattgaaaga tcagctttct gatttactga gactgacttg
     1501 gccggaagaa tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg
     1561 aatcgcggta gcagcggtag accaagatga gttagtagga tttattggtg caatccctca
     1621 atacggtatc acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa
     1681 ccaaataggt actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat
     1741 cacgatttat ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct
     1801 gtatgagcat acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga
     1861 attctatgaa aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga
     1921 caaaccggat atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa
     1981 agaggttgtg acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt
//
LOCUS       DACCKU010000279         1970 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.201, whole genome shotgun sequence.
ACCESSION   DACCKU010000279 DACCKU010000000
VERSION     DACCKU010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.201"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            47..439
                     /locus_tag="IVN13_003164"
     CDS             47..439
                     /locus_tag="IVN13_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP7013559.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            440..1879
                     /locus_tag="IVN13_003165"
                     /note="aph(2'')-Ia"
     CDS             440..1879
                     /locus_tag="IVN13_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAP7013560.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          819 a          149 c          337 g          665 t
ORIGIN      
        1 aacattgtat agcattttac acaggagtct ggacttgact gagtttatgg aagaagtttt
       61 aattgatgat aatatggttt ttgatattga taatttaaaa ggatttctta atgataccag
      121 ttcatttggg tttatagcta aagaaaataa taaaattata ggatttgcat attgctatac
      181 acttttaaga cctgatggaa aaacaatgtt ttatttacac tcaataggaa tgttacctaa
      241 ctatcaagac aaaggttatg gttcaaaatt attatctttt attaaggaat attctaaaga
      301 gattggttgt tctgaaatgt ttttaataac tgataaaggt aatcctagag cttgccatgt
      361 atatgaaaaa ttaggtggta aaaatgatta taaagatgaa atagtatatg tatatgatta
      421 tgaaaaaggt gataaataaa tgaatatagt tgaaaatgaa atatgtataa gaactttaat
      481 agatgatgat tttcctttga tgttaaaatg gttaactgat gaaagagtat tagaatttta
      541 tggtggtaga gataaaaaat atacattaga atcattaaaa aaacattata cagagccttg
      601 ggaagatgaa gtttttagag taattattga atataacaat gttcctattg gatatggaca
      661 aatatataaa atgtatgatg agttatatac tgattatcat tatccaaaaa ctgatgagat
      721 agtctatggt atggatcaat ttataggaga gccaaattat tggagtaaag gaattggtac
      781 aagatatatt aaattgattt ttgaattttt gaaaaaagaa agaaatgcta atgcagttat
      841 tttagaccct cataaaaata atccaagagc aataagggca taccaaaaat ctggttttag
      901 aattattgaa gatttgccag aacatgaatt acacgagggc aaaaaagaag attgttattt
      961 aatggaatat agatatgatg ataatgccac aaatgttaag gcaatgaaat atttaattga
     1021 gcattacttt gataatttca aagtagatag tattgaaata atcggtagtg gttatgatag
     1081 tgtggcatat ttagttaata atgaatacat ttttaaaaca aaatttagta ctaataagaa
     1141 aaaaggttat gcaaaagaaa aagcaatata taatttttta aatacaaatt tagaaactaa
     1201 tgtaaaaatt cctaatattg aatattcgta tattagtgat gaattatcta tactaggtta
     1261 taaagaaatt aaaggaactt ttttaacacc agaaatttat tctactatgt cagaagaaga
     1321 acaaaatttg ttaaaacgag atattgccag ttttttaaga caaatgcacg gtttagatta
     1381 tacagatatt agtgaatgta ctattgataa taaacaaaat gtattagaag agtatatatt
     1441 gttgcgtgaa actatttata atgatttaac tgatatagaa aaagattata tagaaagttt
     1501 tatggaaaga ctaaatgcaa caacagtttt tgagggtaaa aagtgtttat gccataatga
     1561 ttttagttgt aatcatctat tgttagatgg caataataga ttaactggaa taattgattt
     1621 tggagattct ggaattatag atgaatattg tgattttata tacttacttg aagatagtga
     1681 agaagaaata ggaacaaatt ttggagaaga tatattaaga atgtatggaa atatagatat
     1741 tgagaaagca aaagaatatc aagatatagt tgaagaatat tatcctattg aaactattgt
     1801 ttatggaatt aaaaatatta aacaggaatt tatcgaaaat ggtagaaaag aaatttataa
     1861 aaggacttat aaagattgat tatataatat atgaaaagct attataaaag acattagtat
     1921 taaatagttt aaaaaaattc aaaatctttc agagaagctg ttaaaggaat
//
LOCUS       DACCKU010000280         1349 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.220, whole genome shotgun sequence.
ACCESSION   DACCKU010000280 DACCKU010000000
VERSION     DACCKU010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.220"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IVN13_003166"
                     /pseudo
     CDS             46..297
                     /locus_tag="IVN13_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IVN13_003167"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IVN13_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP7013561.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
LOCUS       DACCKU010000281          793 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.245, whole genome shotgun sequence.
ACCESSION   DACCKU010000281 DACCKU010000000
VERSION     DACCKU010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.245"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            3..740
                     /gene="erm(B)"
                     /locus_tag="IVN13_003168"
     CDS             3..740
                     /gene="erm(B)"
                     /locus_tag="IVN13_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7013562.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTITYEQVLSIFNSYLLFNGRK"
BASE COUNT          297 a          138 c          125 g          233 t
ORIGIN      
        1 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
       61 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      121 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      181 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa
      241 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      301 atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccgtgcgt
      361 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      421 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      481 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      541 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      601 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      661 taaacaattt aagtaccatt acttatgagc aagtattgtc tatttttaat agttatctat
      721 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt
      781 actaaaggga atg
//
LOCUS       DACCKU010000282          793 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.246, whole genome shotgun sequence.
ACCESSION   DACCKU010000282 DACCKU010000000
VERSION     DACCKU010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..793
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.246"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            3..740
                     /gene="erm(B)"
                     /locus_tag="IVN13_003169"
     CDS             3..740
                     /gene="erm(B)"
                     /locus_tag="IVN13_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP7013563.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          296 a          138 c          126 g          233 t
ORIGIN      
        1 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc
       61 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta
      121 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag
      181 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa
      241 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga
      301 atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccgtgcgt
      361 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa
      421 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg
      481 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca
      541 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc
      601 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag
      661 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat
      721 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt
      781 actaaaggga atg
//
LOCUS       DACCKU010000283          651 bp    DNA     linear   BCT 10-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate RPH_EFM_188
            SAMN12916640-rid10367873.guided.258, whole genome shotgun sequence.
ACCESSION   DACCKU010000283 DACCKU010000000
VERSION     DACCKU010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12916640
            Sequence Read Archive: SRR10233759
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Enterococcaceae; Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (25-AUG-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 25-AUG-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000820314.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000820314.1
            Genome Coverage       :: 50x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 08/25/2020 17:56:10
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,170
            CDSs (total)                      :: 3,105
            Genes (coding)                    :: 3,035
            CDSs (with protein)               :: 3,035
            Genes (RNA)                       :: 65
            rRNAs                             :: 2, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 70
            CDSs (without protein)            :: 70
            Pseudo Genes (ambiguous residues) :: 0 of 70
            Pseudo Genes (frameshifted)       :: 36 of 70
            Pseudo Genes (incomplete)         :: 29 of 70
            Pseudo Genes (internal stop)      :: 19 of 70
            Pseudo Genes (multiple problems)  :: 12 of 70
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12916640-rid10367873.guided.258"
                     /isolate="RPH_EFM_188"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /geo_loc_name="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2013"
                     /collected_by="Murdoch University"
     gene            38..586
                     /gene="aac(6')"
                     /locus_tag="IVN13_003170"
     CDS             38..586
                     /gene="aac(6')"
                     /locus_tag="IVN13_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP7013564.1"
                     /translation="MVISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          235 a          104 c          151 g          161 t
ORIGIN      
        1 ggactgttag aataagcaag aacaaggggg aagacgtatg gtaatcagtg aatttgaccg
       61 taataatcca gtattgaaag atcagctttc tgatttactg agactgactt ggccggaaga
      121 atatggagac agctcggcag aagaagtaga agaaatgatg aatccagaac gaatcgcggt
      181 agcagcggta gaccaagatg agttagtagg atttattggt gcaatccctc aatacggtat
      241 cacaggttgg gaattgcatc cattagttgt agaaagctcc cgacgaaaga accaaatagg
      301 tactcgatta gtcaattact tagaaaaaga agtagcttcc agaggaggaa tcacgattta
      361 tttaggtacg gatgatttag accatggaac aacgttaagt caaacggacc tgtatgagca
      421 tacatttgat aaagtggctt ccatccagaa ccttcgtgaa catccgtatg aattctatga
      481 aaaattaggt tataaaatcg taggtgtctt accaaatgca aatggctggg acaaaccgga
      541 tatttggatg gcaaaaacga ttattcctcg accaaattct caataataaa aagaggttgt
      601 gacagaagta atcagcttca agaaataaga aggaatttac gaaaattact t
//
